CN106414767A - 生物分子的测序装置、系统及方法 - Google Patents

生物分子的测序装置、系统及方法 Download PDF

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Publication number
CN106414767A
CN106414767A CN201580011297.4A CN201580011297A CN106414767A CN 106414767 A CN106414767 A CN 106414767A CN 201580011297 A CN201580011297 A CN 201580011297A CN 106414767 A CN106414767 A CN 106414767A
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China
Prior art keywords
biomolecule
nanochannel
nano
gap electrode
electrode
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Pending
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CN201580011297.4A
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English (en)
Chinese (zh)
Inventor
川合知二
谷口正辉
大城敬人
马克·弗洛伊德·欧德涵姆
埃里克·斯科特·诺德曼
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University of Osaka NUC
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Quantum Biosystems Inc
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Publication of CN106414767A publication Critical patent/CN106414767A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/157—Nanotubes or nanorods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201580011297.4A 2014-01-24 2015-01-23 生物分子的测序装置、系统及方法 Pending CN106414767A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014011430 2014-01-24
JP2014-011430 2014-01-24
PCT/JP2015/052601 WO2015111760A1 (en) 2014-01-24 2015-01-23 Devices, systems and methods for sequencing biomolecules

Publications (1)

Publication Number Publication Date
CN106414767A true CN106414767A (zh) 2017-02-15

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CN201580011297.4A Pending CN106414767A (zh) 2014-01-24 2015-01-23 生物分子的测序装置、系统及方法

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US (1) US20160377591A1 (enExample)
EP (1) EP3097412A1 (enExample)
JP (1) JP6636455B2 (enExample)
KR (1) KR20160130380A (enExample)
CN (1) CN106414767A (enExample)
WO (1) WO2015111760A1 (enExample)

Cited By (3)

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WO2019000158A1 (zh) * 2017-06-26 2019-01-03 武汉科技大学 一种基于隧道识别技术的纳米检测装置及方法
CN111278561A (zh) * 2017-05-16 2020-06-12 威斯康星校友研究基金会 用于整体图案化分子结构的方法和系统
CN113390940A (zh) * 2021-06-03 2021-09-14 浙江大学 一种集成纳米孔的分子隧穿检测装置

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WO2011108540A1 (ja) 2010-03-03 2011-09-09 国立大学法人大阪大学 ヌクレオチドを識別する方法および装置、ならびにポリヌクレオチドのヌクレオチド配列を決定する方法および装置
CN106104274B (zh) 2013-09-18 2018-05-22 量子生物有限公司 生物分子测序装置、系统和方法
JP2015077652A (ja) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 ナノギャップ電極およびその製造方法
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
CA3000942A1 (en) * 2015-10-08 2017-04-13 Quantum Biosystems Inc. Devices, systems and methods for nucleic acid sequencing
CN115595360A (zh) 2016-04-27 2023-01-13 因美纳剑桥有限公司（Gb） 用于生物分子的测量和测序的系统和方法
JP2019525766A (ja) * 2016-08-02 2019-09-12 国立大学法人大阪大学 ナノ電極対の作製及び較正のためのデバイス及び方法
WO2019068034A2 (en) 2017-09-29 2019-04-04 Biothlon, Inc. NANOPORA DEVICE AND METHOD FOR MANUFACTURING THE SAME
WO2019072743A1 (en) 2017-10-13 2019-04-18 Analog Devices Global Unlimited Company DESIGN AND MANUFACTURE OF SENSORS IN NANOGAP
JP2021038923A (ja) * 2017-11-02 2021-03-11 株式会社Ｓｃｒｅｅｎホールディングス 基板
US11067561B2 (en) 2017-12-31 2021-07-20 Palogen, Inc. Nanopore device and methods of electrical array addressing and sensing
JP7372683B2 (ja) * 2018-11-06 2023-11-01 国立大学法人大阪大学 電極基板および電極基板の製造方法
US20200326325A1 (en) * 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
US11779918B2 (en) 2019-12-05 2023-10-10 International Business Machines Corporation 3D nanochannel interleaved devices
JP7780783B2 (ja) * 2021-03-04 2025-12-05 国立大学法人大阪大学 デバイス、トンネル電流測定装置、核酸配列読取装置、トンネル電流測定方法、および、核酸配列読取方法
US20250321204A1 (en) 2024-04-15 2025-10-16 Robert Bosch Gmbh Sensor unit of a nucleic acid analysis system

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CN103502795A (zh) * 2011-03-09 2014-01-08 阿比奥尼克公司 选择性功能化的纳米流体生物传感器中的生物分子的快速定量及其方法

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CN103502795A (zh) * 2011-03-09 2014-01-08 阿比奥尼克公司 选择性功能化的纳米流体生物传感器中的生物分子的快速定量及其方法

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111278561A (zh) * 2017-05-16 2020-06-12 威斯康星校友研究基金会 用于整体图案化分子结构的方法和系统
WO2019000158A1 (zh) * 2017-06-26 2019-01-03 武汉科技大学 一种基于隧道识别技术的纳米检测装置及方法
CN113390940A (zh) * 2021-06-03 2021-09-14 浙江大学 一种集成纳米孔的分子隧穿检测装置
CN113390940B (zh) * 2021-06-03 2022-10-21 浙江大学 一种集成纳米孔的分子隧穿检测装置

Also Published As

Publication number Publication date
JP6636455B2 (ja) 2020-01-29
KR20160130380A (ko) 2016-11-11
WO2015111760A1 (en) 2015-07-30
JP2017509899A (ja) 2017-04-06
US20160377591A1 (en) 2016-12-29
EP3097412A1 (en) 2016-11-30

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