JP6636455B2 - 生体分子配列決定装置、システムおよび方法 - Google Patents

生体分子配列決定装置、システムおよび方法 Download PDF

Info

Publication number
JP6636455B2
JP6636455B2 JP2016565939A JP2016565939A JP6636455B2 JP 6636455 B2 JP6636455 B2 JP 6636455B2 JP 2016565939 A JP2016565939 A JP 2016565939A JP 2016565939 A JP2016565939 A JP 2016565939A JP 6636455 B2 JP6636455 B2 JP 6636455B2
Authority
JP
Japan
Prior art keywords
nanochannel
biomolecule
electrodes
nanogap
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016565939A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017509899A (ja
JP2017509899A5 (enExample
Inventor
川合　知二
知二 川合
正輝 谷口
正輝 谷口
敬人 大城
敬人 大城
フロイド オールダム マーク
フロイド オールダム マーク
スコット ノードマン エリック
スコット ノードマン エリック
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Osaka NUC
Original Assignee
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osaka University NUC filed Critical Osaka University NUC
Publication of JP2017509899A publication Critical patent/JP2017509899A/ja
Publication of JP2017509899A5 publication Critical patent/JP2017509899A5/ja
Application granted granted Critical
Publication of JP6636455B2 publication Critical patent/JP6636455B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/157—Nanotubes or nanorods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2016565939A 2014-01-24 2015-01-23 生体分子配列決定装置、システムおよび方法 Active JP6636455B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014011430 2014-01-24
JP2014011430 2014-01-24
PCT/JP2015/052601 WO2015111760A1 (en) 2014-01-24 2015-01-23 Devices, systems and methods for sequencing biomolecules

Publications (3)

Publication Number Publication Date
JP2017509899A JP2017509899A (ja) 2017-04-06
JP2017509899A5 JP2017509899A5 (enExample) 2019-07-11
JP6636455B2 true JP6636455B2 (ja) 2020-01-29

Family

ID=52469259

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016565939A Active JP6636455B2 (ja) 2014-01-24 2015-01-23 生体分子配列決定装置、システムおよび方法

Country Status (6)

Country Link
US (1) US20160377591A1 (enExample)
EP (1) EP3097412A1 (enExample)
JP (1) JP6636455B2 (enExample)
KR (1) KR20160130380A (enExample)
CN (1) CN106414767A (enExample)
WO (1) WO2015111760A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9194838B2 (en) 2010-03-03 2015-11-24 Osaka University Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
EP3578987A1 (en) 2013-09-18 2019-12-11 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
JP2015077652A (ja) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 ナノギャップ電極およびその製造方法
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
KR20180072717A (ko) * 2015-10-08 2018-06-29 퀀텀 바이오시스템즈 가부시키가이샤 핵산 시퀀싱을 위한 장치, 시스템 및 방법
ES2877193T3 (es) 2016-04-27 2021-11-16 Quantum Biosystems Inc Sistemas y métodos para la medición y secuenciación de biomoléculas
WO2018025887A1 (en) * 2016-08-02 2018-02-08 Quantum Biosystems Inc. Devices and methods for creation and calibration of a nanoelectrode pair
US11213825B2 (en) * 2017-05-16 2022-01-04 Wisconsin Alumni Research Foundation System and method for en masse patterning of molecule structures
WO2019000158A1 (zh) * 2017-06-26 2019-01-03 武汉科技大学 一种基于隧道识别技术的纳米检测装置及方法
CN111194408B (zh) 2017-09-29 2022-12-02 帕洛根公司 纳米孔设备及其制造方法
US11740226B2 (en) 2017-10-13 2023-08-29 Analog Devices International Unlimited Company Designs and fabrication of nanogap sensors
JP2021038923A (ja) * 2017-11-02 2021-03-11 株式会社Ｓｃｒｅｅｎホールディングス 基板
US11067561B2 (en) 2017-12-31 2021-07-20 Palogen, Inc. Nanopore device and methods of electrical array addressing and sensing
JP7372683B2 (ja) * 2018-11-06 2023-11-01 国立大学法人大阪大学 電極基板および電極基板の製造方法
US20200326325A1 (en) * 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
US11779918B2 (en) 2019-12-05 2023-10-10 International Business Machines Corporation 3D nanochannel interleaved devices
JP7780783B2 (ja) * 2021-03-04 2025-12-05 国立大学法人大阪大学 デバイス、トンネル電流測定装置、核酸配列読取装置、トンネル電流測定方法、および、核酸配列読取方法
CN113390940B (zh) * 2021-06-03 2022-10-21 浙江大学 一种集成纳米孔的分子隧穿检测装置
US20250321204A1 (en) 2024-04-15 2025-10-16 Robert Bosch Gmbh Sensor unit of a nucleic acid analysis system

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
IL148088A0 (en) * 1999-08-13 2002-09-12 Us Genomics Inc Methods and apparatuses for stretching polymers
WO2001063273A2 (en) * 2000-02-22 2001-08-30 California Institute Of Technology Development of a gel-free molecular sieve based on self-assembled nano-arrays
US8232582B2 (en) * 2000-04-24 2012-07-31 Life Technologies Corporation Ultra-fast nucleic acid sequencing device and a method for making and using the same
CN103203256B (zh) * 2006-07-19 2015-09-23 博纳基因技术有限公司 纳米口装置阵列:它们的制备以及在大分子分析中的应用
US8969090B2 (en) * 2010-01-04 2015-03-03 Life Technologies Corporation DNA sequencing methods and detectors and systems for carrying out the same
US8986524B2 (en) * 2011-01-28 2015-03-24 International Business Machines Corporation DNA sequence using multiple metal layer structure with different organic coatings forming different transient bondings to DNA
PT2684027T (pt) * 2011-03-09 2020-01-27 Abionic Sa Rápida quantificação de biomoléculas num biossensor nanofluídico seletivamente funcionalizado e seu método
JP2013090576A (ja) * 2011-10-24 2013-05-16 Hitachi Ltd 核酸分析デバイス及びそれを用いた核酸分析装置

Also Published As

Publication number Publication date
KR20160130380A (ko) 2016-11-11
EP3097412A1 (en) 2016-11-30
JP2017509899A (ja) 2017-04-06
WO2015111760A1 (en) 2015-07-30
US20160377591A1 (en) 2016-12-29
CN106414767A (zh) 2017-02-15

Similar Documents

Publication Publication Date Title
JP6636455B2 (ja) 生体分子配列決定装置、システムおよび方法
US10557167B2 (en) Biomolecule sequencing devices, systems and methods
US10413903B2 (en) Devices, systems and methods for linearization of polymers
US20190242846A1 (en) Devices and methods for creation and calibration of a nanoelectrode pair
US20190071720A1 (en) Devices, systems and methods for nucleic acid sequencing
JP2019164142A (ja) ナノギャップ電極およびその製造方法
US8003319B2 (en) Systems and methods for controlling position of charged polymer inside nanopore
US20170306396A1 (en) Electrodes for biomolecular sequencing device, and biomolecular sequencing device, method, and program
JP2017517009A (ja) 異種材料を含むナノギャップ電極
JP6761035B2 (ja) 生体分子の分析方法および分析装置

Legal Events

Date Code Title Description
AA64 Notification of invalidation of claim of internal priority (with term)

Free format text: JAPANESE INTERMEDIATE CODE: A241764

Effective date: 20161220

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170130

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180122

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20180122

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190205

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20190401

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20190405

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20190605

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20191122

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20191218

R150 Certificate of patent or registration of utility model

Ref document number: 6636455

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250