KR20150002018A - Composition comprising Panax ginseng polysaccharide and Green tea polysaccharide for improving skin trouble - Google Patents

Abstract

In the present invention, provided is a composition for improving troubles on skin, comprising 0.001-10 wt% of each polysaccharide of ginseng and of green tea for the total weight percentage of the composition as active ingredients. When the polysaccharide of ginseng and of green tea are used together, the polysaccharide of ginseng and of green tea has better effects of blocking ultra-violet rays than the polysaccharide of ginseng and of green tea is separately used.

Description

[0001] The present invention relates to a composition for improving skin trouble containing a ginseng polysaccharide and a green tea polysaccharide,

More particularly, the present invention relates to a composition containing ginseng polysaccharide and green tea polysaccharide and having excellent effect of alleviating inflammation and allergy, improving atopy, and reducing pores.

Skin is a body part that is always exposed directly to the external environment. It functions as a barrier to protect the internal organs from external stimuli, inhibit moisture evaporation, and block the penetration of foreign substances from the outside. However, skin is exposed to toxic chemicals, ozone depletion, ultraviolet irradiation dose, stress, and the like, thereby decreasing the activity of keratinocytes and fibroblasts of the skin, decreasing collagen synthesis ability and elastin synthesis ability, And the ability to defend against infectious agents.

In recent years, especially skin inflammation related to atopy and acne are increasing trends. Accordingly, there is a desperate need to develop a substance that alleviates skin irritation and irritation caused by various irritating agents, reduces skin irritation caused by detergents and synthetic surfactants contained in cosmetics, thereby alleviating skin troubles.

The term atopy refers to chronic eczematous dermatitis, which leads to severe itching and irritation and irritation in the sense of improper or unusual. These atopic dermatitis is experienced by 10-20% of all human beings during infancy, but disappears mostly when older. As these atrophies begin to develop, they become more pruritious as time goes by and they cause irritation and irritation. They cause scratching, scratching, scarring and inflammation. The inflammation is amplified again, do. During this process, the skin is severely damaged to a degree that it is difficult to cure, and it becomes dirty and causes secondary infection by viruses or bacteria.

Acne is caused by a variety of factors, including diet, genetic factors, the environment, stress and hormone imbalance, but ultimately, the secretion of sebum from the skin does not escape from the skin and builds up in the skin. The main cause of this sebaceous gland disease is that accumulated sebum blocks the pores and causes the formation of Propionibacterium acnes ) were found to proliferate. That is, as the secretion of sebum becomes stronger due to the hormone change, the secreted sebum blocks the pores and creates an anaerobic condition in which the bacteria can grow in the pores. During this proliferation of the acne bacterium, a toxic substance that stimulates the skin is secreted, causing inflammation, resulting in the breakdown of the sebaceous glands, the appearance of pus or red rash, and secondary damage to skin health .

Various methods have been attempted to alleviate such skin troubles. Among them, the method of removing a substance causing skin irritation has a limitation because a functional raw material itself acts as a stimulating source in many cases. Accordingly, many studies have been made to neutralize the acidity by adding strong alkalis such as NaOH or KOH to the stimulant-inducing functional materials to mitigate the irritation, but such a prescription has a problem of deteriorating the efficacy of the existing raw materials. Recently, based on a study on the method of inhibiting cell-mediated immunity, neuropeptide antagonists including substances P (substance P), bradykinin antagonists and COX inhibitors have been proposed as substances capable of reducing skin inflammation and stimulation have.

As for the cosmetic for relieving irritation, a composition for alleviating skin irritation caused by ultraviolet rays (Patent Publication No. 2000-0058730), a composition for external application for skin containing raspberry leaf or fruit extract (Patent Publication No. 2003-0015654) A composition containing phytosterol (Japanese Patent Application Laid-Open No. 2002-0063987) and the like, but did not show a clear effect.

Accordingly, the present inventors have found that when a ginseng polysaccharide derived from ginseng and a green tea polysaccharide derived from green tea are simultaneously used, the synergistic effect effectively alleviates inflammation and allergy, improves atopy, reduces pores It was confirmed that the effect was excellent, and the present invention was completed.

Accordingly, it is an object of the present invention to provide a composition excellent in inflammation relief, atopy improvement, and pore reduction effect by using a ginseng polysaccharide and a green tea polysaccharide simultaneously.

In order to accomplish the above object, the present invention provides a composition for external application for skin or a pharmaceutical composition comprising the ginseng polysaccharide and the green tea polysaccharide as an active ingredient.

The composition of the present invention exhibits an effect of alleviating inflammation and improving atopy by containing a ginseng polysaccharide and a green tea polysaccharide. Therefore, the composition containing the ginseng polysaccharide and the green tea polysaccharide can be usefully used for inflammation, allergy reduction, atopic improvement, sebum inhibition and reduction of pores.

The composition of the present invention contains a ginseng polysaccharide and a green tea polysaccharide as an effective ingredient, thereby providing an excellent skin trouble improving effect.

Hereinafter, the present invention will be described more specifically.

The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. In addition, arabinose, rhamnose, glucose and And galactose constitute side chains. The ginseng used in the present invention is a plant belonging to the genus Ogushi and ginseng. In the present invention, ginseng can be used without limitation in its kind and root length, and both dried and not dried ginseng can be used. In the present invention, ginseng can be used without limitation in the site, and specifically, roots of ginseng can be used.

The green tea polysaccharide used in the present invention is derived from green tea and is an acidic polysaccharide group, unlike a polysaccharide found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis. In the present invention, green tea can be used regardless of the type and the number of days of cultivation, and both dried and non-dried green tea can be used. In the present invention, the green tea can be used without limitation in the area, and specifically, the leaves of green tea can be used.

The ginseng polysaccharide and the green tea polysaccharide used in the present invention can be produced by a method known in the art, and the method is not particularly limited. Specifically, a green tea polysaccharide or a ginseng polysaccharide can be obtained by preparing a ginseng root or green tea leaf extract with water at 38 to 42 캜, concentrating it, removing the impurities with a filter, adding ethanol dropwise thereto and drying with hot air .

The composition of the present invention may contain the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.005 to 9.5% 0.09 to 7.5 wt.%, 0.1 to 6.5 wt.%, 0.3 to 6 wt.%, 0.5 to 5.5 wt.%, Or 0.7 By weight to 5% by weight.

In the present invention, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. In order to maximize the synergistic effect of the combination of the two components, preferably, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1 .

The skin trouble of the present invention encompasses cases where the skin is not in a normal state due to various causes. Specifically, it includes, but is not limited to, acne, atopy, rash or allergy, and includes swollen or crusted skin.

The composition for improving skin trouble according to the present invention may have anti-inflammatory or anti-allergic ability. The composition can alleviate skin irritation and alleviate inflammation, and thus can be used for anti-inflammation.

The composition for improving skin troubles according to the present invention can alleviate allergy or inhibit the mechanism of the body which is a cause of allergy, thereby treating or alleviating allergy. Specifically, the composition can inhibit or inhibit the production of prostaglandin itself, or inhibit or inhibit the activity of prostaglandins. The composition may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces prostaglandins. The composition may inhibit or inhibit the production of interleukin itself or may inhibit or inhibit the activity of interleukin. The composition may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. Specifically, the interleukin may include interleukin-6 or interleukin-8, but is not limited thereto. Therefore, the composition of the present invention may inhibit or inhibit the expression of prostaglandin or interleukin, thereby resulting in an anti-allergic effect.

The composition for improving skin trouble according to the present invention can treat or improve atopy. The composition can enhance the skin barrier function and induce the differentiation of dermal keratinocytes. Therefore, the composition can be used for the treatment of atopic dermatitis or atopic dermatitis which is caused by abnormal skin barrier and the like.

The composition for improving skin trouble according to the present invention may have antibacterial activity. Specifically, the composition exhibits excellent antibacterial activity against acne bacteria. Therefore, the composition can be effectively used in the treatment and alleviation of acne.

The composition for improving skin trouble according to the present invention means to alleviate skin troubles or skin diseases caused by various reasons, to improve the condition, or to remove or inhibit various reasons causing skin troubles or skin diseases, Or can improve the skin condition causing it, which is useful for improving the skin condition.

Also, the composition for improving skin trouble according to the present invention can be used as a composition for shrinking pores and controlling sebum, and it can suppress the excess sebum secreted when applied to the skin, reduce active oxygen and promote collagen synthesis, Is excellent.

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Comparative Example 1] Production of ginseng polysaccharide

1 kg of dried white ginseng was pulverized with a blender, and then 10 L of purified water was added thereto, followed by stirring at 38 to 42 ° C in hot water for 24 hours, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 65 g of ginseng polysaccharide.

[Comparative Example 2] Production of green tea polysaccharide

1 kg of first processed green tea leaves were pulverized with a blender, 10 l of purified water was added thereto, and the mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise and the mixture was hot-air dried to obtain 72 g of a green tea polysaccharide.

[Example 1] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 2 were mixed at a weight ratio of 1: 3.

[Example 2] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 2 were mixed at a weight ratio of 1: 1.

[Example 3] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 2 were mixed at a weight ratio of 3: 1.

 [Test Example 1] Inflammation improving effect

Before the experiment, the skin keratinocyte (NHEK, available from Lonza) was dispensed into a 96-well plate at 5 × 10 ⁴ cells / well and incubated at 37 ° C in a 5% CO ₂ incubator incubator) for 24 hours. Twenty-four hours later, the cells were washed twice with PBS and replaced with serum free keratinocyte basement media (KBM). Each of the wells was treated with a mixture of the ginseng polysaccharide and the green tea polysaccharide of Comparative Examples 1 and 2 and Examples 1 to 3 at the respective concentrations shown in Table 1 and reacted for 30 minutes. PGSA (10 μg / ml) Lt; / RTI > Here, peptidoglycan from S. aureus (PGSA ) is a peptidoglycan extracted from Staphylococcus aureus , which is a major constituent of the cell wall of Gram-positive (+) bacteria, and the cell membrane components of bacteria are known to cause inflammation, Especially in staphylococcus, about 90% of atopic patients are reported to cause secondary infection by this bacterium. After culturing in a 5% CO ₂ incubator at 37 ° C for 24 hours, the culture was taken and ELISA for interleukin-8 (IL-8) was performed. The results are shown in Table 1 below. The ELISA used the experimental method of BD science.

Test substance IL-8 secretion (pg / ml) Untreated Control (Control) 755.44 PGSA (10 [mu] g / ml) 4215.46 Comparative Example 1 (25 ppm) + PGSA (10 μg / ml) 3905.21 Comparative Example 1 (50 ppm) + PGSA (10 占 퐂 / ml) 3422.58 Comparative Example 2 (25 ppm) + PGSA (10 占 퐂 / ml) 2975.94 Comparative Example 2 (50 ppm) + PGSA (10 占 퐂 / ml) 2483.67 Example 1 (25 ppm) + PGSA (10 占 퐂 / ml) 2535.90 Example 1 (50 ppm) + PGSA (10 占 퐂 / ml) 1950.34 Example 2 (25 ppm) + PGSA (10 占 퐂 / ml) 2656.13 Example 2 (50 ppm) + PGSA (10 占 퐂 / ml) 2194.05 Example 3 (25 ppm) + PGSA (10 占 퐂 / ml) 2698.27 Example 3 (50 ppm) + PGSA (10 占 퐂 / ml) 2202.48

As shown in Table 1, it was confirmed that the composition of the present invention significantly reduced or suppressed the secretion of IL-8 increased by PGSA and LPS. Particularly, it was confirmed that the example had remarkably high IL-8 secretion inhibiting effect than the comparative example using each of the ginseng polysaccharide or the green tea polysaccharide. Thus, it can be seen that the composition of the present invention significantly reduces the secretion of IL-8 by PGSA, thereby providing excellent anti-inflammatory effects.

[Test Example 2] Evaluation of itching relief

At the end of the experiment, keratinocytes (cell line name: HaCaT, available from ATCC) were dispensed in a 96 well plate at ⁴ x ¹⁰ cells / well and incubated at 37 ° C in a 5% CO ₂ incubator And cultured for 24 hours. Twenty-four hours later, a 96-well plate was washed twice with HBSS (Hanks Balanced Salt solution) buffer, and the reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM probenecid) . After incubation at 37 ° C in a 5% CO ₂ incubator for 30 minutes and at room temperature for 30 minutes, the cells were washed twice with HBSS buffer, and the cells (Comparative Examples 1 to 2 and Examples 1 to 3) Lt; / RTI >

After reaction for 10 minutes to process the 2U / ml trypsin (Trypsin) or 5μM PAR-2 activation peptide (SLIGKV) and was measured in Ca ^{2 +} concentration in the cell 80 seconds. Measurement of intracellular Ca ²⁺ concentration was performed using FlexStation 3 (Molecular Device, USA). A mixture of the ginseng polysaccharide and the green tea polysaccharide of Comparative Examples 1 to 2 and Examples 1 to 3 was measured for 80 seconds after treatment with 2 U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) The difference between the minimum value and the maximum value was obtained and compared with the difference between the minimum value and the maximum value at the time of treatment with 2 U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) to determine the intracellular influx of calcium ions The inhibition rate (%) is shown in Table 2 below.

Test substance concentration Inhibition rate of intracellular influx of calcium ions (%) Trypsin (2 U / ml) PAR-2 active peptide (5 [mu] M) Comparative Example 1 0.1% 14 17 0.5% 16 19 Comparative Example 2 0.1% 19 22 0.5% 22 26 Example 1 0.05% 25 28 0.1% 28 32 0.5% 34 38 Example 2 0.05% 24 26 0.1% 27 31 0.5% 33 37 Example 3 0.05% 22 25 0.1% 26 31 0.5% 33 36

As shown in Table 2, the composition of the present invention reduces inflow of intracellular calcium ions by trypsin or PAR-2 activating peptide (SLIGKV) and increases intracellular calcium ions remarkably Respectively. Particularly, the composition of the present invention has remarkably higher inhibitory effect on the influx of calcium ions than the comparative example using only the ginseng polysaccharide or the green tea polysaccharide. Thus, it can be seen that the composition of the present invention effectively inhibits the itching-inducing PAR-2 activity, thereby providing a superior antinociceptive effect.

[Test Example 3] Test for antimicrobial activity against acne bacteria

Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 4 were prepared according to the ingredients and the contents (% by weight) shown in Table 3 below. Specifically, Formulation Examples 1 to 3 were prepared by mixing a mixture of ginseng polysaccharide and green tea polysaccharide, Comparative Formulation Example 3 contained no active ingredient for acne skin improvement, Comparative Formulation Example 4 was prepared as a standard for antibacterial activity (Erythromycin), which is widely used as a treatment for acne as a standard substance.

The manufacturing methods of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 4 are as follows. The components of the phase A in Table 3 were completely dissolved and the components of the phase B were completely dissolved in a separate melting tank, and the phase B was added to the phase A to be mixed and solubilized. The components of the C phase were added thereto in accordance with the mixing ratios shown in Table 3, followed by mixing and homogenization, followed by filtration to prepare the compositions.

The unit of the content ratio of the following ingredients is% by weight.

Composition statement (% by weight) Formulation Example 1 Formulation Example 2 Formulation Example 3 compare
Formulation Example 1 compare
Formulation Example 2 compare
Formulation Example 3 compare
Formulation Example 4 A Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 B ethanol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 PEG-60 hydrogenated castor oil 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Perfume 0.04 0.04 0.04 0.04 0.04 0.04 0.04 C Example 1 5.0 - - - - - - Example 2 - 5.0 - - - - - Example 3 - - 5.0 - - - - Comparative Example 1 - - - 5.0 - - - Comparative Example 2 - - - - 5.0 - - Erythromycin - - - - - - 5.0

The antibacterial activity against each of the compositions prepared in the formulations of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 4 was tested against propionibacterium acnes (ATCC 6919: medium-BHI broth), which is a cause of acne .

The test method for the antimicrobial activity against acne bacteria is as follows.

(1) Preparation of test strain

Propionibacterium acnes were cultured in an anaerobic culture inoculated on BHI broth.

(2) Dilution solution preparation

0.15 ml of the above test solution was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5), and the mixture was used as a dilute solution.

(3) Preparation of sample

Each of the compositions prepared from Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 4 was used as a sample.

(4) Antimicrobial activity test

1) In a 96-well 96-well plate, place the sample in line 1 and add 200 μl of diluted solution.

2) Thoroughly mix the mixture of line 1, and then take 100 μl of the mixture, place it in the second row, mix well, and then repeat the double dilution by adding 100 μl to the third row.

3) After culturing the cells at 32 ° C for 24 hours and 48 hours, determine whether the bacteria are proliferating to the extent of suspension, and determine the minimum concentration without bacterial growth as the MIC (Minimum Inhibitory Concentration) value. If the mixture is opaque and it is difficult to judge the growth of the microorganism, check with a microscope.

The results of the antimicrobial activity test against acne bacteria are shown in Table 4 below. The MIC was expressed in terms of the concentration of the active ingredient contained in the formulation.

Item pH MIC (ppm) Formulation Example 1 5.7 > 65 ppm Formulation Example 2 5.7 > 66 ppm Formulation Example 3 5.7 > 68 ppm Comparative Formulation Example 1 5.7 > 155 ppm Comparative Formulation Example 2 5.7 > 100 ppm Comparative Formulation Example 3 5.7 Maximum concentration (no antimicrobial activity) Comparative Formulation Example 4 5.7 > 82 ppm

The lower the ppm concentration in the MIC, the more effective the antimicrobial activity against acne bacteria. In the case of Formulation Examples 1 to 3 of the present invention, it was confirmed that they have very excellent antibacterial activity because they have a significantly lower ppm concentration than Comparative Formulation Example 4 using erythromycin, which is a known acne treatment agent.

[Test Example 4] Test for improving atopy symptoms in human body

Cream containing ginseng polysaccharide and green tea polysaccharide was prepared with the composition shown in Table 5 below.

Ingredients (% by weight) Formulation Example 4 Formulation Example 5 Formulation Example 6 compare
Formulation Example 5 compare
Formulation Example 6 compare
Formulation Example 7 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Example 1 1.0 - - - - - Example 2 - 1.0 - - - - Example 3 - - 1.0 - - - Comparative Example 1 - - - - 1.0 - Comparative Example 2 - - - - - 1.0 Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 Polyglyceryl-10 < / RTI > pentastearate &
Behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / capric triglyceride 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

The creams of Formulations 4 to 6 and Comparative Formulations 5 to 7 prepared in the composition of Table 5 were applied to the panels to evaluate the degree of improvement of atopic dermatitis. For each of the 90 panelists who thought that there was a symptom of dermatitis showing similar symptoms, each composition was divided into 9 groups of 10 each, and the composition of Table 5 was administered twice daily for 8 weeks at a temperature of 24 to 26 캜 and a humidity of 75% And applied to the face. At the end of the test, subjects were asked to fill out a questionnaire and conducted a subjective efficacy evaluation. In the questionnaire, the symptoms of atopic dermatitis were divided into canopy, dryness, keratinization, dandruff, erythema, swelling, skin cracking, This was averaged to evaluate the improvement effect of atopic symptoms on an individual basis. The results are shown in Table 6.

Application group (number of respondents - persons) Significant improvement Improving No change worse Formulation Example 4 3 5 2 0 Formulation Example 5 3 6 One 0 Formulation Example 6 3 5 2 0 Comparative Formulation Example 5 0 5 5 0 Comparative Formulation Example 6 0 7 3 0 Comparative Formulation Example 7 One 8 One 0

As a result, it was confirmed that Formulation Examples 4 to 6 containing the composition of the present invention had more excellent effects for improving symptoms of atopic dermatitis than Comparative Formulation Example 5. Furthermore, it was confirmed that the formulation containing the composition of the present invention had a superior atopic dermatitis-improving effect than Comparative Formulations 6 to 7 containing Comparative Examples 1 and 2.

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Soft capsule

A soft capsule was prepared by mixing 150 mg of the ginseng polysaccharide of Comparative Example 1, 150 mg of the green tea polysaccharide of Comparative Example 2, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 6 mg of lecithin and filling them with 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

150 mg of the ginseng polysaccharide of Comparative Example 1, 150 mg of the green tea polysaccharide of Comparative Example 2, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added to form granules. And the tablet was tableted using a tablet machine.

[Formulation Example 3] Granules

150 mg of the ginseng polysaccharide of Comparative Example 1, 150 mg of the green tea polysaccharide of Comparative Example 2, 100 mg of glucose, 50 mg of the red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C to form granules And filled in the next bubble. The final weight of the contents was 1 g.

[Formulation Example 4] Softening lotion (skin lotion)

The softening longevity was prepared by a conventional method according to the composition shown in Table 7 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.1 The green tea polysaccharide of Comparative Example 2 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Nutritional lotion (milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 8 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.5 The green tea polysaccharide of Comparative Example 2 0.5 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] Nourishing cream

Nutritive creams were prepared according to the compositions listed in Table 9 below in a conventional manner.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 1.0 The green tea polysaccharide of Comparative Example 2 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 7] Massage cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 10 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 1.5 The green tea polysaccharide of Comparative Example 2 1.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 8] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 11 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.1 The green tea polysaccharide of Comparative Example 2 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 9] ointment

Ointments were prepared in a conventional manner according to the compositions shown in Table 12 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.1 The green tea polysaccharide of Comparative Example 2 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A composition for external application for skin for improving skin trouble containing ginseng polysaccharide and green tea polysaccharide as an active ingredient. [2] The composition for external application for skin according to claim 1, wherein the ginseng polysaccharide and the green tea polysaccharide are contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. A pharmaceutical composition for improving skin trouble containing ginseng polysaccharide and green tea polysaccharide as an active ingredient. [Claim 5] The pharmaceutical composition according to claim 4, wherein the ginseng polysaccharide and the green tea polysaccharide are contained in an amount of 0.001-10 wt% based on the total weight of the composition. [Claim 5] The pharmaceutical composition according to claim 4, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1.