KR20130109974A - 식물체에서 ｄｈａ 및 다른 ｌｃ-ｐｕｆａ의 생산 - Google Patents

식물체에서 ｄｈａ 및 다른 ｌｃ-ｐｕｆａ의 생산 Download PDF

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KR20130109974A
KR20130109974A KR1020127033006A KR20127033006A KR20130109974A KR 20130109974 A KR20130109974 A KR 20130109974A KR 1020127033006 A KR1020127033006 A KR 1020127033006A KR 20127033006 A KR20127033006 A KR 20127033006A KR 20130109974 A KR20130109974 A KR 20130109974A
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acid sequence
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cell
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테렌스 에이. 월시
앤 오언스 메를로
다니엘 가쇼테
폴 고든 로슬러
스코트 베번
제리 엠. 쿠너
제임스 지. 메츠
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다우 아그로사이언시즈 엘엘씨
디에스엠 아이피 어셋츠 비.브이.
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Abstract

본 발명은, 숙주 생물체에서 PUFA의 생산을 허용 및/또는 개선시키는 다중불포화 지방산(PUFA) 신타제 시스템 및 하나 이상의 보조 단백질(예: PPTase 및/또는 ACoAS)로 유전자 변형된 재조합 숙주 생물체(예: 식물)를 제공한다. 본 발명은 또한 이러한 생물체로부터 수득한 생성물(예: 오일 및/또는 종자) 뿐만 아니라 이러한 생물체를 제조하고 사용하는 방법(PUFA를 수득하기 위해)에 관한 것이다.

Description

식물체에서 ＤＨＡ 및 다른 ＬＣ-ＰＵＦＡ의 생산{PRODUCTION OF DHA AND OTHER LC-PUFAS IN PLANTS}
본 발명은, 숙주 생물체에서 PUFA의 생산을 허용 및/또는 개선시키는 다중불포화 지방산(PUFA) 신타제 시스템 및 하나 이상의 보조 단백질로 유전자 변형된 재조합 숙주 생물체(예: 식물체)를 제공한다. 본 발명은 또한 이러한 생물체로부터 수득한 생성물(예: 오일 및/또는 종자) 뿐만 아니라 이러한 생물체를 제조하고 사용하는 방법(PUFA를 수득하기 위해)에 관한 것이다.
다중불포화 지방산(PUFA)은 영양 분야, 약제 분야, 산업 분야 및 기타 목적에 유용한 것으로 간주된다. 그러나, 천연 공급원(예: 어유(fish oils)) 및 화학 합성으로부터 PUFA의 현재 공급은 장기간의 상업적 요구를 위해 충분하지 않다.
식물(예: 오일 종자 작물)로부터 유래된 식물성 오일은 비교적 저렴하고, 어유와 관련된 오염 문제를 갖지 않는다. 그러나, 상업적으로 개발된 식물 및 식물유에서 발견된 PUFA는 더욱 포화된 또는 보다 장쇄의 PUFA를 통상 포함하지 않고, 리놀레산(델타 9 및 12 위치에서, 2개의 이중결합을 갖는 18개 탄소 -- 18:2 델타 9,12) 및 리놀렌산(18:3 델타 9,12,15) 등의 지방산을 통상 유일하게 포함한다.
식물체에 의해 내생적으로 생산된 지방산의 변형에 의한 식물체에서 더욱 불포화된 또는 보다 장쇄의 PUFA의 생산은 기재되어 있다. 예를 들면, 지방산 신장효소(elongase) 및/또는 탈포화효소(desaturase)를 코딩하는 다양한 개개 유전자를 사용한 식물체의 유전자 변형은 에이코사펜타엔산(EPA) 등의 보다 장쇄 및 더욱 불포화된 PUFA를 상당한 수준으로 함유하지만 혼합된 보다 단쇄 및 덜 불포화된 PUFA를 또한 상당한 수준으로 함유하는 잎 또는 종자를 생성하는 것으로 기재되어 있다[참조: Qi et al., Nature Biotech. 22:739 (2004); WO 04/071467; Abbadi et al., Plant Cell 16:1 (2004); Napier and Sayanova, Proceedings of the Nutrition Society 64:387-393 (2005); Robert et al., Functional Plant Biology 32:473-479 (2005); 미국 특허출원 공개번호 2004/0172682].
브라시카(Brassica) 속은 세계의 가장 중요한 지방종자 작물 중의 하나인 캐놀라를 포함하고, 대부분의 지방종자 작물은 적당한 지형에서 재배했다. 캐놀라는 전통적으로, 에루스산 및 글루코시놀레이트가 제거되거나 통상적인 육종을 통해 현저히 감소된, 브라시카 나푸스(Brassica napus)(브라시카 라파(Brassica rapa)와 브라시카 올레라세아(Brassica oleracea)의 종간 교잡의 결과로서 유래된 종)으로서 특성화되어 있다. 대부분의 캐놀라 오일은 인간 소비를 위해 생산된 식물유 형태이다. 또한, 산업 분야에서는 캐놀라 오일의 사용을 위한 시장이 성장하고 있다.
캐놀라 종자의 특정 품종으로부터 유래된 식용유 및 산업유의 품질은, 지방산 불포화의 유형 및 양이 식이 및 산업 분야 둘 다에 대한 관련성을 갖기 때문에, 이의 구성 지방산에 의해 결정된다. 통상의 캐놀라 오일은 약 60% 올레산(C18:1), 약 20%의 리놀레산(C18:2) 및 약 10%의 리놀렌산(18:3)을 함유한다. 통상적 캐놀라에 전형적인 다중불포화 리놀렌산의 수준은 당해 오일이 용이하게 산화되기 때문에 바람직하지 않고, 산화 속도는 산소의 존재, 광 및 열에의 노출, 및 오일 중의 천연 또는 첨가된 항산화제 및 산화촉진제의 존재를 포함하는 몇몇 인자에 의해 영향을 받는다. 산화는 반복된 프라잉(frying)(유도된 산화) 또는 장기간 저장(자동-산화)의 결과로서 불쾌한 냄새 및 악취를 유발한다. 산화는 또한 캐놀라 오일의 윤활 및 점성 특성을 변화시킬 수 있다.
통상의 캐놀라 오일과 비교하여 다중불포화 지방산의 수준 감소 및 모노불포화 올레산의 수준 증가를 나타내는 오일은 보다 높은 산화 안정성과 관련된다. 산화에 대한 개개 지방산의 감수성은 이들의 불포화도에 의존한다. 따라서, 3개의 탄소-탄소 이중결합을 보유하는 리놀렌산의 산화 속도는 단지 하나의 이중결합을 갖는 올레산 산화 속도의 25배이고, 2개의 이중결합을 갖는 리놀레산 산화 속도의 2배이다. 리놀레산 및 리놀렌산은 또한, 이들이 용이하게 과산화물을 형성하기 때문에, 냄새 및 향기에 대해 대부분의 영향을 갖는다. 높은 올레산 오일(≥70% 올레산)은 저장, 프라잉 및 정련(refining) 동안 산화에 덜 감수성이고, 발연 없이 보다 고온으로 가열하여 조리유로서 더욱 적합할 수 있다. 종자 오일(seed oil) 중의 지방산 프로파일이 올레산(C18:1) 70%(중량 기준) 초과 및 리놀렌산(C18:3) 3.5%(중량 기준) 미만을 갖는 상업적으로 판매된 캐놀라 품종의 예는 다우 아그로사이언시스 엘엘씨(Dow AgroSciences LLC)사(Indianapolis, IN)에서 판매된 NexeraTM 품종이고, 당해 품종은, 딥 프라잉(deep frying), 소테(sauteing), 베이킹, 스프레잉을 포함하는 다수의 분야 및 샐러드 드레싱에서 레스토랑 및 외식 산업에 의해 현재 사용된 "오메가-9 오일", 비수소화된, 높은 올레산, 낮은 리놀렌산 오일을 생산한다.
당해 기술분야에서는, 식물체, 식물 종자 또는 식물 오일에서 이러한 PUFA가 풍부한 지질(예: 트리아실글리세롤(TAG) 및 인지질(PL))의 양 뿐만 아니라 식물체, 식물 종자 또는 식물 오일에서 보다 장쇄 또는 더욱 불포화된 PUFA의 양(예: 상업적 양)을 효율적 및 효과적으로 생산하기 위한 비교적 저렴한 방법이 요구되고 있다. 본원에 기재된 다중불포화 지방산(PUFA) 신타제 및 하나 이상의 보조 단백질로 유전자 변형된 재조합 숙주 생물체를 제공함으로써 숙주 생물체(예: 식물체)에서 PUFA 생산을 제공하고 개선시키는 시스템은 당해 기술분야에서 상기 접근법에 대한 중요한 대안이다.
본 발명은, (i) 적어도 하나의 PUFA를 생산하는 다중불포화 지방산(PUFA) 신타제 시스템(예: 조류 PUFA 신타제 시스템)을 인코딩하는 핵산 서열 및 (ii) 포스포판테테이닐 보조인자를 PUFA 신타제 시스템(예: 조류 PUFA 신타제 시스템) ACP 도메인으로 전달하는 포스포판테테이닐 트랜스퍼라제(PPTase)를 코딩하는 핵산 서열을 포함하는, 유전자 변형된 식물체(예: 브라시카), 이의 후대, 종자, 세포, 조직, 또는 부분에 관한 것이다. 일부 양태에서, 유전자 변형된 식물, 이의 후대, 종자, 세포, 조직 또는 부분은 경제적으로 중요한 브라시카 종[예: 브라시카 나푸스(Brassica napus) 또는 브라시카 준세아(Brassica juncea)로부터 유래한다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 1의 아미노산 서열과 적어도 60% 내지 99% 동일한 아미노산 서열을 포함하거나, 서열번호 1의 아미노산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 6의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함하건, 서열번호 6의 핵산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 2의 아미노산 서열과 적어도 60% 내지 99% 동일한 아미노산 서열을 포함하거나, 서열번호 2의 아미노산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템을 코딩하는 핵산 서열은 서열번호 7의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함하거나, 서열번호 7의 핵산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 3의 아미노산 서열과 적어도 60% 내지 99% 동일한 아미노산 서열을 포함하거나, 서열번호 3의 아미노산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 8의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함하거나, 서열번호 8의 핵산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 1, 2 또는 3의 아미노산 서열 또는 이의 임의의 조합을 포함한다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 6, 7 또는 8의 핵산 서열 또는 이의 임의의 조합을 포함한다.
일부 양태에서, PPTase는 서열번호 5와 적어도 60% 내지 99% 동일한 아미노산 서열을 포함하거나, 서열번호 5의 아미노산 서열을 포함한다. 일부 양태에서, PPTase를 인코딩하는 핵산 서열은 서열번호 10의 핵산 서열과 적어도 60% 내지 99% 동일하거나, 서열번호 10의 핵산 서열을 포함한다.
일부 양태에서, 핵산 서열(i) 및 (ii)는 단일 재조합 발현 벡터에 함유되어 있다. 일부 양태에서, 핵산 서열(i) 및 (ii)는 종자 특이적 프로모터에 작동적으로 연결되어 있다. 일부 양태에서, 핵산 서열(i) 및 (ii)는 PvDlec2, PvPhaseolin, LfKCS3 및 FAE 1로 이루어진 그룹으로부터 선택된 프로모터에 작동적으로 연결되어 있다.
일부 양태에서, 유전자 변형된 식물(예: 캐놀라 오일을 생산하는 브라시카 종), 이의 후대, 종자, 세포, 조직 또는 부분은 (iii) 장쇄 PUFA 유리 지방산(PFFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신타제(ACoAS)를 인코딩하는 핵산 서열을 추가로 포함한다. 일부 양태에서, ACoAS는 서열번호 4와 적어도 60% 내지 99% 동일한 아미노산 서열을 포함하거나, 서열번호 4의 아미노산 서열을 포함한다. 일부 양태에서, ACoAS는 서열번호 9의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함하거나, 서열번호 9의 핵산 서열을 포함한다. 일부 양태에서, ACoAS를 인코딩하는 핵산 서열은 서열번호 34의 핵산 서열을 포함한다. 일부 양태에서, 핵산 서열(i), (ii) 및/또는 (iii)은 단일 재조합 발현 벡터에 함유되어 있다.일부 양태에서, 핵산 서열(i), (ii) 및/또는 (iii)은 종자 특이적 프로모터에 작동적으로 연결되어 있다. 일부 양태에서, 핵산 서열(i), (ii) 및/또는 (iii)은 PvDlec2, LfKCS3 및 FAE 1로 이루어진 그룹으로부터 선택된 프로모터에 작동적으로 연결되어 있다.
일부 양태에서, 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분은 아세틸 CoA 카복실라제(ACCase)를 인코딩하는 핵산 서열 및/또는 타입 2 디아실글리세롤 아실트랜스퍼라제(DGAT2)를 인코딩하는 핵산 서열을 추가로 포함한다.
본 발명은 서열번호 6 내지 10 및 서열번호 34로부터 선택된 핵산 서열, 재조합 발현 벡터 pDAB7361, 재조합 발현 벡터 pDAB7362, 재조합 발현 벡터 pDAB7363, 재조합 발현 벡터 pDAB7365, 재조합 발현 벡터 pDAB7368, 재조합 발현 벡터 pDAB7369, 재조합 발현 벡터 pDAB7370, 재조합 발현 벡터 pDAB100518, 재조합 발현 벡터 pDAB101476, 재조합 발현 벡터 pDAB9166, 재조합 발현 벡터 pDAB9167, 재조합 발현 벡터 pDAB7379, 재조합 발현 벡터 pDAB7380, 재조합 발현 벡터 pDAB9323, 재조합 발현 벡터 pDAB9330, 재조합 발현 벡터 pDAB9337, 재조합 발현 벡터 pDAB9338, 재조합 발현 벡터 pDAB9344, 재조합 발현 벡터 pDAB9396, 재조합 발현 벡터 pDAB101412, 재조합 발현 벡터 pDAB7733, 재조합 발현 벡터 pDAB7734, 재조합 발현 벡터 pDAB101493, 재조합 발현 벡터 pDAB109507, 재조합 발현 벡터 pDAB109508, 재조합 발현 벡터 pDAB109509, 재조합 발현 벡터 pDAB9151, 재조합 발현 벡터 pDAB108207, 재조합 발현 벡터 pDAB108208, 재조합 발현 벡터 pDAB108209, 재조합 발현 벡터 pDAB9159, 재조합 발현 벡터 pDAB9147, 재조합 발현 벡터 pDAB108224 또는 재조합 발현 벡터 pDAB108225를 포함하는 분리된 핵산 분자에 관한 것이다.
일부 양태에서, 유전자 변형된 식물, 이의 후대, 종자, 세포, 조직 또는 부분으로부터 수득된 종자 오일은 검출가능한 양의 DHA(도코사헥사엔산(C22:6, n-3)) 및/또는 EPA(에이코사펜타엔산(C20:5, n-3))를 포함한다. 일부 양태에서, 종자 오일은 0.01% 내지 15% DHA, 0.05% 내지 10% DHA, 또는 0.05% 내지 5% DHA를 포함한다. 일부 양태에서, 종자 오일은 0.01% 내지 5% EPA, 0.05% 내지 5% EPA, 또는 0.05% 내지 1% EPA를 포함한다. 다른 양태에서, 종자 오일에서 발견된 검출가능한 양의 DHA 및/또는 EPA는 또한 유전자 변형된 식물로부터 수득된 낟알 및/또는 굵게 빻은 가루에서 발견된다. 일부 양태에서, 검출가능한 양의 DHA 및/또는 EPA는 70중량% 이상의 올레산(C18:1) 및/또는 4중량% 이하의 리놀렌산(C18:3)을 포함하는 지방산 함량을 갖는 브라시카 종의 종자 오일에서 발견된다.
본 발명은 본원에 기재된 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분으로부터 수득된 오일 또는 종자에 관한 것이다. 본 발명은 본원에 기재된 유전자 변형된 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 오일을 포함하는 식료품에 관한 것이다. 본 발명은 또한 본원에 기재된 유전자 변형된 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 오일, 또는 본원에 기재된 유전자 변형된 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 종자를 포함하는 기능성 식품에 관한 것이다. 본 발명은 본원에 기재된 유전자 변형된 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 오일을 포함하는 약품에 관한 것이다.
본 발명은, 본원에 기재된 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분으로부터 또는 본원에 기재된 유전자 변형된 식물(예: 브라시카)의 종자, 이의 후대, 세포, 조직 또는 부분으로부터 오일을 회수하는 단계를 포함하는, 적어도 하나의 LC-PUFA를 포함하는 오일의 생산 방법에 관한 것이다. 본 발명은 또한, 본원에 기재된 유전자 변형된 식물체(예: 브라시카), 이의 후대, 세포, 조직 또는 부분을 성장시키는 단계를 포함하는, 적어도 하나의 LC-PUFA를 포함하는 오일의 생산 방법에 관한 것이다. 본 발명은 또한 본원에 기재된 유전자 변형된 식물(예: 브라시카)의 종자, 이의 후대, 세포, 조직 또는 부분으로부터 오일을 회수하는 단계를 포함하는, 종자 오일에서 적어도 하나의 LC-PUFA를 생산하는 방법에 관한 것이다.
본 발명은, 본원에 기재된 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분을 성장시키는 포함하는, 종자 오일에서 적어도 하나의 PUFA를 생산하는 방법에 관한 것이다. 본 발명은 또한, 본원에 기재된 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분, 본원에 기재된 오일, 본원에 기재된 종자, 본원에 기재된 식료품, 본원에 기재된 기능성 식품, 또는 본원에 기재된 약품을 개체에게 제공하는 단계를 포함하는, 적어도 하나의 PUFA를 함유하는 보충 또는 치료 생성물을 개체에게 제공하는 방법에 관한 것이다. 일부 양태에서, 이러한 양태에 함유된 PUFA는 DHA 및/또는 EPA이다.
본 발명은 또한, 식물 또는 식물 세포를, (i) 적어도 하나의 다중불포화 지방산(PUFA)을 생산하는 PUFA 신타제 시스템(예: 조류 PUFA 신타제 시스템)을 인코딩하는 핵산 서열; 및 (ii) 포스포판테테이닐 보조인자를 PUFA 신타제 시스템(예: 조류 PUFA 신타제 시스템) ACP 도메인으로 전달하는 포스포판테테이닐 트랜스퍼라제(PPTase)를 인코딩하는 핵산 서열로 형질전환시킴을 포함하는, 본원에 기재된 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분을 생산하는 방법에 관한 것이다. 일부 양태에서, 상기 방법은 식물 또는 식물 세포를 (iii) 장쇄 PUFA 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신타제(ACoAS)를 인코딩하는 핵산 서열로 형질전환시킴을 추가로 포함한다.
본 발명의 다양한 양태는, 본 발명의 일부를 형성하는, 하기 상세한 설명, 도면 및 첨부 서열 기재로부터 보다 충분히 이해될 수 있다.
도 1은 PUFA OrfA의 9개 반복 도메인 각각을 인코딩하는 재설계된 DNA 서열의 클러스털(Clustal) W(벡터 NTI에 정렬)를 도시한다.
도 2는 pDAB7361의 플라스미드 맵을 나타낸다.
도 3은 pDAB7362의 플라스미드 맵을 나타낸다.
도 4는 pDAB7363의 플라스미드 맵을 나타낸다.
도 5는 캐놀라 이벤트 5197[14]-032.002로부터 T1 종자의 DHA 함량의 단일 종자 분석을 나타낸다.
도 6은 Orf A, Orf B 및 Orf C 특이적 항혈청으로 탐침된 캐놀라 이벤트 5197[14]-032.002로부터의 후기(>30 DAP) 발육 T1 종자의 추출물의 SDS-PAGE 웨스턴 블롯의 결과를 나타낸다.
도 7a는 DHA 생성 캐놀라 이벤트 5197[14]-032.002.Sx002로부터의 수분(pollination) 후 15, 20, 25, 30, 35 및 42일에서 수집된 발육 T2 종자 시료의 지질 함량을 나타낸다.
도 7b는 웨스턴 블롯에 의해 DHA 생성 캐놀라 이벤트 5197[14]-032.002.Sx002로부터의 추출물에 OrfA, OrfB 및 OrfC 폴리펩티드의 존재를 나타낸다.
도 8은 캐놀라 이벤트 5197[14]-032.002의 온실 성장 T1 식물체로부터 호모접합성 T2 식물체의 LC-PUFA 함량을 나타낸다.
도 9는 6개 호모접합성 라인으로부터 단일 T2 종자 분석물의 LC-PUFA의 요약을 나타낸다.
도 10은 2개의 T1 라인과 비형질전환된 오메가-9 넥세라(Nexera) 710의 역교잡으로부터 수득된 모(parent) 및 F1 하이브리드 종자의 DHA 함량을 나타낸다.
도 11은 캐놀라 이벤트 5197[13]-010.001로부터 유래된 60개 개개의 T1 식물체의 pat 유전자 카피 수를 나타낸다.
도 12는 수분 후 일수(DAP)로서 나타낸 6개 시점 각각에 대한 원(raw) 강도 값을 사용하여 눌(null) 비형질전환 오메가-9 넥세라 710 라인에서 목적하는 유전자의 발현 프로파일을 나타낸다.
도 13은 DAP로서 나타낸 6개 시점 각각에 대한 표준 강도 값을 사용하여 눌 비형질전환 오메가-9 넥세라 710 라인에서 목적하는 유전자의 발현 프로파일을 나타낸다.
도 14는 DAP로서 나타낸 6개 시점 각각에 대한 원 강도 값을 사용하여 호모접합성 이벤트 5197[14]-032.002 라인에서 목적하는 유전자의 발현 프로파일을 나타낸다.
도 15는 DAP로서 나타낸 6개 시점 각각에 대한 표준 강도 값을 사용하여 호모접합성 이벤트 5197[14]-032.002 라인에서 목적하는 유전자의 발현 프로파일을 나타낸다.
도 16은 박층 크로마토그래피(TLC)에 의해 측정한 성숙 유전자변형 캐놀라 종자에서 PUFA 신타제 활성을 나타낸다.
도 17은 공-발현된 HetI의 존재 또는 부재하에 이. 콜라이(E. coli)에서 발현된 OrfA, 및 캐놀라 이벤트 5197[14]-032.002에서 발현된 OrfA로부터 서로에 대한 참조 펩티드의 계산된 비율을 나타낸다.
도 18은 HetI의 존재 또는 부재하에 이. 콜라이에서 발현된 OrfA, 및 유전자변형 캐놀라 이벤트 5197[14]-032.002에서 발현된 OrfA로부터 6개 참조 펩티드 각각에 대한 apo2-9 펩티드의 계산된 비율을 나타낸다.
도 19는 pDAB7365의 플라스미드 맵을 나타낸다.
도 20은 pDAB7368의 플라스미드 맵을 나타낸다.
도 21은 pDAB7369의 플라스미드 맵을 나타낸다.
도 22는 pDAB7370의 플라스미드 맵을 나타낸다.
도 23은 pDAB100518의 플라스미드 맵을 나타낸다.
도 24는 pDAB101476의 플라스미드 맵을 나타낸다.
도 25는 pDAB101477의 플라스미드 맵을 나타낸다.
도 26은 pDAB9166의 플라스미드 맵을 나타낸다.
도 27은 pDAB9167의 플라스미드 맵을 나타낸다.
도 28은 pDAB7379의 플라스미드 맵을 나타낸다.
도 29는 pDAB7380의 플라스미드 맵을 나타낸다.
도 30은 pDAB9323의 플라스미드 맵을 나타낸다.
도 31은 pDAB9330의 플라스미드 맵을 나타낸다.
도 32는 pDAB9337의 플라스미드 맵을 나타낸다.
도 33은 pDAB9338의 플라스미드 맵을 나타낸다.
도 34는 pDAB9344의 플라스미드 맵을 나타낸다.
도 35는 pDAB9396의 플라스미드 맵을 나타낸다.
도 36은 pDAB101412의 플라스미드 맵을 나타낸다.
도 37은 pDAB7733의 플라스미드 맵을 나타낸다.
도 38은 pDAB7734의 플라스미드 맵을 나타낸다.
도 39는 pDAB101493의 플라스미드 맵을 나타낸다.
도 40은 pDAB109507의 플라스미드 맵을 나타낸다.
도 41은 pDAB109508의 플라스미드 맵을 나타낸다.
도 42는 pDAB109509의 플라스미드 맵을 나타낸다.
도 43은 pDAB9151의 플라스미드 맵을 나타낸다.
도 44는 pDAB108207의 플라스미드 맵을 나타낸다.
도 45는 pDAB108208의 플라스미드 맵을 나타낸다.
도 46은 pDAB108209의 플라스미드 맵을 나타낸다.
도 47은 pDAB9159의 플라스미드 맵을 나타낸다.
도 48은 pDAB9147의 플라스미드 맵을 나타낸다.
도 49는 pDAB108224의 플라스미드 맵을 나타낸다.
도 50은 pDAB108225의 플라스미드 맵을 나타낸다.
도 51은 pDAB101493, pDAB7362, pDAB7369, pDAB101412 또는 pDAB7380로 형질전환시킨 개개의 유전자변형 아라비돕시스(Arabidopsis) 이벤트로부터 T2 종자의 DHA 및 LC-PUFA 함량을 설명한다.
본원에 사용된 용어 "다중불포화 지방산(polyunsaturated fatty acid)" 또는 "PUFA"는 적어도 3개 이상의 이중결합, 4개 이상의 이중결합, 5개 이상의 이중결합, 6개 이상의 이중결합(여기서, 모든 이중결합은 cis 배열로 존재함)와 함께 적어도 16개 탄소, 적어도 18개 탄소, 적어도 20개 탄소, 또는 22개 이상의 탄소의 탄소쇄 길이를 갖는 지방산을 지칭한다.
본원에 사용된 용어 "장쇄 다중 불포화 지방산" 또는 "LC-PUFA"는 3개 이상의 이중결합, 4개 이상의 이중결합, 5개 이상의 이중결합, 6개 이상의 이중결합을 함유하는, 18개 이상의 탄소쇄 길이, 20개 이상의 탄소쇄 길이의 지방산을 지칭한다. 오메가-6 시리즈의 LC-PUFA는, 이로써 한정되는 것은 아니지만, 감마-리놀렌산(C18:3), 디-호모-감마-리놀렌산(C20:3n-6), 아라키돈산(C20:4n-6), 아드렌산(또는 도코사테트라엔산 또는 DTA라 불리움)(C22:4n-6) 및 도코사펜타엔산(C22:5n-6)을 포함한다. 오메가-3 시리즈의 LC-PUFA는, 이로써 한정되는 것은 아니지만, 알파-리놀렌산(C18:3), 에이코사트리엔산(C20:3n-3), 에이코산테트라엔산(C20:4n-3), 에이코산펜타엔산(C20:5n-3), 도코사펜타엔산(C22:5n-3) 및 도코사헥사엔산(C22:6n:3)을 포함한다. LC-PUFA는 또한 22개 초과의 탄소 및 4개 이상의 이중결합을 갖는 지방산을 포함하고, 이로써 한정되지는 않지만, C28:8(n-3)을 포함한다.
본원에 사용된 용어 "PUFA 신타제" 또는 "PUFA 신타제 시스템" 또는 "SzPUFA" 또는 "hSzThPUFA"는 다중불포화 지방산(PUFA) 및 특히 장쇄 PUFA(LC-PUFA) 뿐만 아니라 복합체 중의 이러한 효소의 임의의 도메인을 생산하는 효소 시스템을 지칭한다. 용어 PUFA 신타제는, 이로 한정되지 않지만, PUFA PKS 시스템 또는 PUFA 생산용 PKS-형 시스템을 포함한다.
본원에 사용된 용어 "포스포판테테이닐 트랜스퍼라제" 또는 "PPTase" 또는 "NoHetl"은 보조인자(예: 4-포스포판테테인)를 보조효소 A(Coa)로부터 PUFA 신타제 시스템에 존재하는 하나 이상의 ACP 도메인으로 전달함으로써 PUFA 신타제 시스템을 활성화시키는 효소를 지칭한다.
본원에 사용된 용어 "아실-CoA 합성효소" 또는 "ACoAS" 또는 "SzACS-2"는 장쇄 다중불포화 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 효소를 지칭한다.
본원에 사용된 용어 "식물"은, 이로 한정되지 않지만, 식물의 임의의 후대, 세포, 조직 또는 부분을 포함한다.
"기능식품(Nutraceutical)"은, 향상된 수준의 이러한 생리학적 활성 성분을 함유하도록 유전자 조작된 작물로부터 생산된 식품과 함께, 이러한 생성물이 보충된 가공 식품을 포함하여, 생리학적 잇점을 제공하거나 질병으로부터의 보호를 제공하는 식물로부터 분리, 정제, 농축 또는 생산된 생성물을 의미한다.
"기능성 식품"은, (a) 통상의 식이의 일부로서 소비되는 통상의 식품과 유사하거나 통상의 식품일 수 있고 (b) 비변형 식품에 전형적으로 존재하는 성분의 비율로 변형에 기반한 향상된 영양가 및/또는 특정한 식이 잇점을 갖는 식품을 의미한다.
용어 "폴리뉴클레오티드" 및 "핵산"은 단일 핵산 뿐만 아니라 복수 핵산, 핵산 분자 또는 단편, 변이체 또는 이의 유도체, 또는 작제물, 예를 들면, 메신저 RNA(mRNA) 또는 플라스미드 DNA(pDNA)를 포함하는 것으로 의도된다. 폴리뉴클레오티드 또는 핵산은, 비해독 5' 및 3' 서열 및 당해 인코딩 서열을 포함하는 전장 cDNA 서열의 뉴클레오티드 서열 또는 이의 단편을 함유할 수 있다. 폴리뉴클레오티드 또는 핵산은 임의의 폴리리보뉴클레오티드 또는 폴리데옥시리보뉴클레오티드로 구성될 수 있고, 이는 비변형 RNA 또는 DNA이거나 변형 RNA 또는 DNA일 수 있다. 예를 들면, 폴리뉴클레오티드 또는 핵산은 단일쇄 및 이중쇄 DNA, 단일쇄 및 이중쇄 영역의 혼합물인 DNA, 단일쇄 및 이중쇄 RNA, 단일쇄 및 이중쇄 영역의 혼합물인 RNA, 단일쇄가거나 보다 통상적으로는 이중쇄가거나 단일쇄 및 이중쇄 영역의 혼합물일 수 있는 DNA 및 RNA를 포함하는 하이브리드 분자로 구성될 수 있다. 이들 용어는 또한 폴리뉴클레오티드 또는 핵산의 화학적, 효소적 또는 대사적 변형된 형태를 포함한다.
폴리뉴클레오티드 또는 핵산 서열은, 이의 천연 환경으로부터 제거된 "분리된" 것으로 지칭될 수 있다. 예를 들면, 벡터에 함유된 디하이드록시-산 데하이드라타제 활성을 갖는 폴리펩티드 또는 폴리펩티드 단편을 인코딩하는 이종성 폴리뉴클레오티드 또는 핵산은 본 발명의 목적을 위해 분리된 것으로 간주된다. 분리된 폴리뉴클레오티드 또는 핵산의 추가의 예는 이종성 숙주 세포에 유지된 재조합 폴리뉴클레오티드 또는 용액 중의 정제된(부분적 또는 실질적) 폴리뉴클레오티드 또는 핵산을 포함한다. 본 발명에 따르는 분리된 폴리뉴클레오티드 또는 핵산은 추가로 합성에 의해 생산된 이러한 분자를 포함한다. DNA의 폴리머 형태의 분리된 폴리뉴클레오티드 또는 핵산은 cDNA, 게놈 DNA 또는 합성 DNA의 하나 이상의 세그먼트로 구성될 수 있다.
용어 "유전자"는, 코딩 서열을 선행(5' 비-코딩 서열) 및 후행(3' 비-코딩 서열)하는 조절 서열을 임의로 포함하는, 특이적 단백질로서 발현될 수 있는 핵산 또는 이의 단편을 지칭한다.
본원에 사용된 바와 같이, 용어 "코딩 영역"은 특이적 아미노산 서열을 코딩하는 DNA 서열을 지칭한다. "적합한 조절 서열"은 코딩 서열의 상류(5' 비-코딩 서열), 코딩 서열 내 또는 코딩 서열의 하류(3' 비-코딩 서열)에 위치되고 전사, RNA 프로세싱 또는 안정성, 또는 관련 코딩 서열의 해독에 영향을 미치는 뉴클레오티드 서열을 지칭한다. 조절 서열은 프로모터, 번역 리더(translation reader) 서열, 인트론, 폴리아데닐화 인지 서열, RNA 프로세싱 부위, 효과기 결합 부위 및 머리핀 구조(stem-loop structure)를 포함할 수 있다.
본원에 사용된 바와 같이, 용어 "폴리펩티드"는 복수의 "폴리펩티드" 및 이의 단편 뿐만 아니라 단일 "폴리펩티드"를 포함하는 것으로 의도되고, 아미드 결합(또한 펩티드 결합으로 공지됨)에 의해 선형 연결된 단량체(아미노산)로 구성된 분자를 지칭한다. 용어 "폴리펩티드"는 2개 이상의 아미노산의 임의의 쇄 또는 쇄들을 지칭하고, 생성물의 특정 길이를 지칭하지 않는다. 따라서, 2개 이상의 아미노산의 쇄 또는 쇄들을 지칭하기 위해 사용된 펩티드, 디펩티드, 트리펩티드, 올리고펩티드, 단백질, 아미노산 쇄 또는 임의의 기타 용어는 "폴리펩티드"의 정의에 포함되고, 용어 "폴리펩티드"는 이들 임의의 용어 대신에 또는 이와 상호교환적으로 사용될 수 있다. 폴리펩티드는 천연 생물학적 공급원으로부터 유래될 수 있거나 재조합 기술에 의해 생산될 수 있지만, 설계된 핵산 서열로부터 반드시 해독되는 것은 아니다. 이는 화학적 합성에 의한 것을 포함하는 임의의 방식으로 생성될 수 있다.
"분리된" 폴리펩티드 또는 이의 단편, 변이체 또는 유도체란 이의 자연 환경에 존재하지 않는 폴리펩티드를 의도한다. 어떠한 특정 수준의 정제는 요구되지 않는다. 예를 들면, 분리된 폴리펩티드는 이의 천연 또는 자연 환경으로부터 제거될 수 있다. 숙주 세포에서 발현된 재조합에 의해 생산된 폴리펩티드 및 단백질은 본 발명의 목적상 분리된 것으로 간주되는데, 이는 임의의 적합한 기술에 의해 분리, 분획을 위해, 또는 부분적 또는 실질적으로 정제된 천연 또는 재조합 폴리펩티드이기 때문이다.
본원에 사용된 바와 같이, "천연(native)"은, 존재하는 경우, 자체의 조절 서열과 함께 자연에서 발견된 폴리뉴클레오티드, 유전자 또는 폴리펩티드의 형태를 지칭한다.
본원에 사용된 바와 같이, "내생적"은 생물체의 자연 위치 또는 생물체의 게놈중 천연 형태의 폴리뉴클레오티드, 유전자 또는 폴리펩티드를 지칭한다. "내생적 폴리펩티드"는 생물체의 게놈 중에서 이의 자연 위치의 천연 폴리뉴클레오티드를 포함한다. "내생적 유전자"는 생물체의 게놈 중에 이의 자연 위치의 천연 유전자를 포함한다. "내생적 폴리펩티드"는 생물체의 자연 위치의 천연 폴리펩티드를 포함한다.
본원에 사용된 바와 같이, "이종성"은, 숙주 생물체에서는 통상 발견되지 않지만 숙주 생물체 내로 도입된 폴리뉴클레오티드, 유전자 또는 폴리펩티드를 지칭한다. "이종성 폴리뉴클레오티드"는, 상응하는 천연 폴리뉴클레오티드와는 상이한 형태로 공급 생물체 내로 재도입된 천연 코딩 영역 또는 이의 부분을 포함한다. "이종성 유전자"는, 상응하는 천연 유전자와는 상이한 형태로 공급 생물체 내로 재도입된 천연 코딩 영역 또는 이의 부분을 포함한다. 예를 들면, 이종성 유전자는, 천연 숙주 내로 재도입된 비천연 조절 영역을 포함하는 키메라 유전자의 일부인 천연 코딩 영역을 포함할 수 있다. "이종성 폴리펩티드"는, 상응하는 천연 폴리펩티드와는 상이한 형태로 공급 생물체 내로 재도입된 천연 폴리펩티드를 포함한다.
본원에 사용된 바와 같이, 용어 "변형(modification)"은, 당해 폴리펩티드의 감소된, 실질적으로 제거된 또는 제거된 활성을 생성하는 본원에 기재된 폴리펩티드의 변화 뿐만 아니라, 당해 폴리뉴클레오티드에 의해 인코딩된 폴리펩티드의 감소되거나, 실질적으로 제거된 또는 제거된 활성을 생성하는 본원에 기재된 폴리뉴클레오티드의 변화를 지칭한다. 이러한 변화는 당해 기술분야에 공지된 방법으로 이루어질 수 있고, 이는, 이로 한정되지 않지만, 결실, 돌연변이화(예: 자발적 돌연변이, 랜덤 돌연변이, 돌연변이유발 유전자에 의해 유발된 돌연변이, 또는 트랜스포손 돌연변이), 치환, 삽입, 하향-조절, 세포 위치의 변화, 폴리뉴클레오티드 또는 폴리펩티드 상태의 변화(예: 메틸화, 포스포릴화 또는 편재단백화(ubiquitination)), 보조인자의 제거, 안티센스 RNA/DNA의 도입, 간섭 RNA/DNA의 도입, 화학적 변형, 공유 변형, UV 또는 X선의 조사, 상동성 재조합, 유사분열 재조합, 프로모터 치환 방법 및/또는 이들의 조합을 포함한다. 뉴클레오티드 또는 아미노산 잔기가 변형될 수 있는지를 측정하는 지침은 특정 폴리뉴클레오티드 또는 폴리펩티드의 서열을 상동성 폴리뉴클레오티드 또는 폴리펩티드의 서열(예: 효모 또는 박테리아)과 비교하고 고도의 상동성(보존된 영역) 또는 컨센서스 서열의 영역에서 이루어진 변형의 수를 최대화함으로써 밝혀질 수 있다.
본원에 사용된 용어 "유도체"는 본 발명에 기재된 서열의 변형을 지칭한다. 이러한 변형의 실례는, 오일 종자 작물 종에서 본원에 기재된 코딩 서열의 기능을 보존, 약간 변경 또는 증가시키는 본원에 기재된 코딩 서열의 핵산 서열과 관련한 하나 이상의 염기의 치환, 삽입 및/또는 결실일 수 있다. 이러한 유도체는, 예를 들면, 서열 구조를 예측 및 최적화하는 컴퓨터 모델링 기술을 사용하여 당업자에 의해 용이하게 측정될 수 있다. 따라서, 용어 "유도체"는, 이들이 본 발명의 LC-PUFA의 생산에 사용하기 위한 개시된 기능성을 가질 수 있도록 본원에 개시된 코딩 서열과 실질적 서열 상동성을 갖는 핵산 서열을 포함한다.
본원에 사용된 바와 같이, 용어 "변이체"는, 예를 들면, 돌연변이 등의 재조합 DNA 기술을 사용하여 생성한 아미노산 삽입, 결실, 돌연변이 및 치환에 의해 본 발명의 구체적으로 기재된 폴리펩티드와는 상이한 폴리펩티드를 지칭한다. 아미노산 잔기가 목적하는 활성의 소실 없이 치환, 부가 또는 결실될 수 있는지를 결정하는 지침은, 특정 폴리펩티드의 서열을 상동성 폴리펩티드의 서열과 비교하고 고도의 상동성(보존된 영역)의 영역에서 이루어진 아미노산 서열 변화의 수를 최적화하거나 아미노산을 컨센서스 서열로 치환시킴으로써 밝혀질 수 있다.
대안적으로, 이들 동일 또는 유사한 폴리펩티드를 인코딩하는 재조합 폴리뉴클레오티드 변이체는 유전자 코드의 "중복성"을 사용함으로써 합성 또는 선별될 수 있다. 다양한 제한 부위를 생성하는 점적 변화(silent change) 등의 다양한 코돈 치환을 도입하여, 발현을 위한 플라스미드 또는 바이러스 벡터 내로의 클로닝을 최적화시킬 수 있다. 폴리뉴클레오티드 서열 내의 돌연변이는 폴리펩티드의 임의 부분의 특성을 변형시키기 위해 당해 폴리펩티드에 부가된 폴리펩티드 또는 다른 펩티드의 도메인에서 반영될 수 있다.
아미노산 "치환"은 하나의 아미노산을 유사한 구조적 및/또는 화학적 특성을 갖는 또 다른 아미노산으로 치환한 결과, 즉 보존적 아미노산 치환일 수 있거나, 하나의 아미노산을 상이한 구조적 및/또는 화학적 특성을 갖는 아미노산으로 치환한 결과, 즉 비보존적 아미노산 치환일 수 있다. "보존적" 아미노산 치환은 관련 잔기의 극성, 전하, 용해도, 소수성, 친수성 또는 양쪽성 성질의 유사성에 기초하여 이루어질 수 있다. 예를 들면, 비극성(소수성) 아미노산은 알라닌, 류신, 이소류신, 발린, 프롤린, 페닐알라닌, 트립토판 및 메티오닌을 포함하고; 극성 중성 아미노산은 글리신, 세린, 트레오닌, 시스테인, 티로신, 아스파라긴 및 글루타민을 포함하고; 양으로 하전된 (염기성) 아미노산은 아르기닌, 리신 및 히스티딘을 포함하고; 음으로 하전된 (산성) 아미노산은 아스파르트산 및 글루탐산을 포함한다. 또는, "비보존적" 아미노산 치환은 이들 임의의 아미노산의 극성, 전하, 용해도, 소수성, 친수성 또는 양쪽성 성질의 차이를 선별함으로써 이루어질 수 있다. "삽입" 또는 "결실"은 재조합 단백질에 의해 구조적 또는 기능적으로 허용되는 변이 범위 내에서 이루어질 수 있다. 허용된 변이는, 재조합 DNA 기술을 사용하여 폴리펩티드 분자에서 아미노산의 삽입, 결실 또는 치환을 시스템적으로 생기게 하고 생성 재조합 변이체를 활성에 대해 분석함으로써 실험적으로 측정할 수 있다.
용어 "프로모터"는 코딩 서열 또는 작용성 RNA의 발현을 조절할 수 있는 DNA 서열을 지칭한다. 일반적으로, 코딩 서열은 프로모터 서열의 3' 위치에 위치된다. 프로모터는 이들의 전체가 천연 유전자로부터 유래될 수 있거나, 자연에서 발견된 상이한 프로모터로부터 유래된 상이한 요소로 구성될 수 있거나, 심지어 합성 DNA 세그먼트를 포함할 수 있다. 당해 기술분야의 통상의 지식을 가진 자는, 상이한 프로모터가 상이한 조직 또는 세포 유형에서, 또는 상이한 발단 단계에서, 또는 상이한 환경적 또는 생리학적 조건에 반응하여 유전자의 발현을 유도할 수 있음을 이해할 것이다. 대부분의 대부분의 세포 유형에서 유전자를 발현시키는 프로모터는 통상 "구성 프로모터"로서 지칭된다. 추가로, 대부분의 경우에 조절 서열의 정확한 경계는 완전히 규정되지 않았기 때문에, 상이한 길이의 DNA 단편이 동일한 프로모터 활성을 가질 수 있는 것으로 인정된다.
용어 "작동적으로 연결된"은, 서열의 작용이 서로에 의해 영향을 받도록 단일 핵산 단편 상의 핵산 서열의 회합을 지칭한다. 예를 들면, 프로모터는, 당해 코딩 서열의 발현에 영향을 미칠 수 있는 경우, 코딩 서열과 작동적으로 연결된다(예를 들면, 코딩 서열은 당해 프로모터의 전사 조절하에 있다). 코딩 서열은 센스 또는 안티센스 배향으로 조절 서열에 작동적으로 연결될 수 있다.
본원에 사용된 용어 "발현"은 본 발명의 핵산 단편으로부터 유래된 센스(mRNA) 또는 안티센스 RNA의 전사 및 안정한 축적을 지칭한다. 발현은 또한 폴리펩티드에서 mRNA의 해독을 지칭할 수 있다.
본원에 사용된 용어 "과발현"은, 동일한 또는 관련 유전자의 내생적 발현보다 높은 발현을 지칭한다. 이종성 유전자는, 이의 발현이 필적하는 내생적 유전자의 발현보다 높은 경우에 과발현된다.
본원에 사용된 바와 같이, 용어 "형질전환"은, 유전적으로 안정한 유전을 발생시키는, 숙주 생물체 내로 핵산 또는 단편의 전달을 지칭한다. 형질전환된 핵산 단편을 함유하는 숙주 생물체는 "유전자변형" 또는 "재조합" 또는 "형질전환된" 생물체로서 지칭된다.
본원에 사용된 용어 "플라스미드" 또는 "벡터"는, 세포의 중심 대사 부분이 아니고 통상 환형 이중쇄 DNA 분자 형태로 존재하는 유전자를 종종 포함하는 염색체외 요소를 지칭한다. 이러한 요소는, 임의로 공급원으로부터 유래된, 단일쇄 또는 이중쇄 DNA 또는 RNA의 자가 복제 서열, 게놈 통합 서열, 파지 또는 뉴클레오티드 서열(선형 또는 환형)일 수 있고, 여기서 다수의 뉴클레오티드 서열은, 적절한 3' 비해독 서열을 따라 선별된 유전자 생성물에 대한 프로모터 단편 및 DNA 서열을 세포 내로 도입할 수 있는 독특한 구성으로 결합되거나 재조합된다.
본원에 사용된 바와 같이 용어 "코돈 축퇴(degeneracy)"는, 인코딩된 폴리펩티드의 아미노산 서열에 영향을 미치지 않고서, 뉴클레오티드 서열의 변이를 허용하는 유전자 코드의 성질을 지칭한다. 당업자는 소정 아미노산을 특성화하기 위해 뉴클레오티드 코돈의 사용에 있어서 특이적 숙주 세포에 의해 나타난 "코돈-바이어스"를 인식한다. 따라서, 숙주 세포에서 개선된 발현을 위해 유전자를 합성할 때, 이의 코돈 사용 빈도가 숙주 세포의 바람직한 코돈 사용 빈도에 접근하도록 유전자를 설계하는 것이 바람직하다.
다양한 숙주 세포의 형질전환을 위한 핵산 분자의 유전자 또는 코딩 영역을 지칭하는 경우의 용어 "코돈 최적화"는, DNA에 의해 인코딩된 폴리펩티드를 변화시키지 않고서, 숙주 생물체의 전형적인 코돈 사용을 반영하도록 핵산 분자의 유전자 또는 코딩 영역 중의 코돈의 변화를 지칭한다. 이러한 최적화는 적어도 하나, 하나 이상 또는 상당 수의 코돈을, 당해 생물체의 유전자에서 보다 빈번히 사용되는 하나 이상의 코돈으로 치환하는 것을 포함한다.
임의의 폴리펩티드 쇄의 아미노산을 인코딩하는 코돈을 포함하는 뉴클레오티드 서열의 편차는 유전자를 인코딩하는 서열의 변이를 허용한다. 각각의 코돈은 3개 뉴클레오티드로 구성되고 DNA를 포함하는 뉴클레오티드가 4개 특정 염기로 제한되기 때문에, 아미노산을 인코딩하는 가능한 뉴클레오티드 조합은 61개 존재한다(나머지 3개 코돈은 해독 종결 시그널을 인코딩한다). 어떠한 코돈을 인코딩하고 어떠한 아미노산을 복제하는지를 나타내는 "유전자 코드"는 표 1로서 본원에서 제공된다. 결과적으로, 다수의 아미노산은 하나 이상의 코돈에 의해 지정된다. 예를 들면, 아미노산 알라닌 및 프롤린은 4개의 트리플릿에 의해 인코딩되고, 세린 및 아르기닌은 6개의 트리플릿으로 인코딩되는 반면, 트립토판 및 메티오닌은 하나의 트리플릿으로 인코딩된다. 이러한 축퇴는, DNA에 의해 인코딩된 단백질의 단백질의 아미노산 서열을 변화시키지 않고서, DNA 염기 조성을 다양한 범위에 걸쳐 달라지게 한다.
표 1. 표준 유전자 코드
Figure pct00001
다수의 생물체는 성장하는 펩티드 쇄에서 특정 아미노산의 삽입용 코드에 대해 특정 코돈을 사용하기 위한 바이어스(bias)를 나타낸다. 생물체간의 코돈 사용에 있어서 차이, 코돈 선호 또는 코돈 바이어스, 유전자 코드의 축퇴에 의해 제공되고, 다수의 생물체 중에서 잘 증명되어 있다. 코돈 바이어스는 종종 메신저 RNA의 해독 효율과 관련되며, 이는 또한, 특히 해독되는 코돈의 특성 및 특정한 전이 RNA(tRNA) 분자의 능력에 의존하는 것으로 생각된다. 세포에서 선택된 tRNA의 우세는 일반적으로 펩티드 합성에 가장 빈번히 사용된 코돈의 반영이다. 따라서, 유전자는 코돈 최적화에 기반하여 소정 생성물에서 최적 유전자 발현을 위해 조정될 수 있다.
다양한 동물, 식물 및 미생물 종에 이용가능한 다수의 유전자 서열이 제공되면, 코돈 사용의 상대적 빈도를 계산할 수 있다. 코돈 사용 표는 용이하게 입수가능하고, 다수의 방식으로 적용될 수 있다[참조: Nakamura et al. Nucl. Acids Res. 28:292 (2000)]. 이러한 또는 유사한 표를 사용함으로써, 당해 기술분야의 통상의 지식을 가진 자는 당해 빈도를 임의의 소정 폴리펩티드 서열에 적용할 수 있고, 폴리펩티드를 인코딩하지만 소정 종에 최적인 코돈을 사용하는 코돈-최적화된 코딩 영역의 핵산 단편을 생산할 수 있다. 본 발명은 본원에 추가로 기재된 바와 같이 본 발명의 OrfA, OrfB, 키메라 OrfC, PPTase 및/또는 기타 보조 단백질의 코돈 최적화된 형태에 관한 것이다.
당해 기술분야에 공지된 용어 "동일성 퍼센트(%)"는, 서열을 비교함으로써 결정되는, 2개 이상의 폴리펩티드 서열 또는 2개 이상의 폴리뉴클레오티드 서열 사이의 관계이다. 당해 기술분야에서, "동일성"은 또한 폴리펩티드 또는 폴리뉴클레오티드 서열 사이의 서열 관련성의 정도를 의미하고, 이 경우에는 이러한 서열 줄(string) 사이의 합치에 의해 결정될 수 있다. "동일성" 및 "유사성"은 공지된 방법으로 용이하게 계산될 수 있고, 이로 한정되는 것은 아니지만, 문헌[참조: 1)　Computational Molecular Biology (Lesk, A. M., Ed.) Oxford University: NY (1988); 2)　Biocomputing: Informatics and Genome Projects (Smith, D. W., Ed.) Academic: NY (1993); 3)　Computer Analysis of Sequence Data, Part I (Griffin, A. M., and Griffin, H. G., Eds.) Humania: NJ (1994); 4)　Sequence Analysis in Molecular Biology (von Heinje, G., Ed.) Academic (1987); and 5)　Sequence Analysis Primer (Gribskov, M. and Devereux, J., Eds.) Stockton: NY (1991)]에 기재된 것들을 포함한다.
동일성을 결정하는 방법은 시험된 서열 사이의 최고 합치를 제공하도록 설계된다. 동일성 및 유사성을 결정하는 방법은 공적으로 이용가능한 컴퓨터 프로그램으로 분류된다. 서열 정렬 및 동일성 퍼센트 계산은, 예를 들면, 벡터 NTI® 쉬트(suite)(Invitrogen, Carsbad, CA)의 AlignX 프로그램 또는 라세르젠(LASERGENE) 생물정보 컴퓨팅 쉬트(DNASTAR Inc., Madison, WI)의 MegAlignTM 프로그램을 사용하여 수행할 수 있다. 당해 서열의 다중 정렬은 클러스털 V로 표지된 정렬 방법(문헌[참조: Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D.G. et al., Comput. Appl. Biosci., 8:189-191 (1992)]에 기재됨)에 상응하는 "클러스털 V 정렬 방법"을 포함하는 몇몇 다양한 알고리즘 변형을 포함하는 "클러스털 정렬 방법"을 사용하여 수행되며, 라세르젠(LASERGENE) 생물정보 컴퓨팅 쉬트(DNASTAR Inc.)의 MegAlignTM 프로그램에서 발견된다. 다중 정렬을 위해, 디폴트 값(default value)은 GAP PENALTY = 10 및 GAP LENGTH PENALTY = 10에 상응한다. 클러스털 방법을 사용한 단백질 서열의 쌍별 정렬 및 동일성 퍼센트 계산을 위한 디폴트 파라미터는 KTUPLE = 1, GAP PENALTY = 3, WINDOW = 5 및 DIAGONALS SAVED = 5이다. 핵산의 경우, 이들 파라미터는 KTUPLE = 2, GAP PENALTY =5, WINDOW = 4 및 DIAGONALS SAVED = 4이다. 클러스털 V 프로그램을 사용한 서열 정렬 후, 동일한 프로그램에서 "서열 거리" 표를 관찰함으로써 "동일성 퍼센트"를 수득할 수 있다. 추가로, "클러스털 W 정렬 방법"을 이용할 수 있고, 클러스털 W로 표지된 정렬 방법(문헌[참조: Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D.G. et al., Comput. Appl. Biosci. 8:189-191(1992)]에 기재됨)에 상응하고, 라세르젠 생물정보 컴퓨팅 쉬트(DNASTAR Inc.)의 MegAlignTM v6.1 프로그램에서 발견된다. 다중 정렬을 위한 디폴트 파라미터(GAP PENALTY=10, GAP LENGTH PENALTY=0.2, Delay Divergen Seqs(%)=30, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB). 클러스털 W 프로그램을 사용한 서열 정렬 후, 동일한 프로그램에서 "서열 거리" 표를 관찰함으로써 "동일성 퍼센트"를 수득할 수 있다.
다수 수준의 서열 동일성이, 이러한 폴리펩티드가 동일하거나 유사한 작용 또는 활성을 갖는 다른 종으로부터 폴리펩티드를 동정하는데 유용하다는 것은 당업자에게 잘 이해된다. 동일성 퍼센트의 유용한 예는, 이로써 한정되지 않지만, 60%, 65%, 70%, 75%, 80%, 85%, 90% 또는 95%를 포함하거나, 60% 내지 100%의 임의의 정수 퍼센트, 예를 들면, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 또는 99%가 본 발명을 기재하는데 유용할 수 있다. 적합한 핵산 단편은 상기 상동성을 가질 뿐만 아니라, 적어도 50개 아미노산, 적어도 100개 아미노산, 적어도 150개 아미노산, 적어도 200개 아미노산 및 적어도 250개 아미노산을 갖는 폴리펩티드를 통상 인코딩한다.
용어 "서열 분석 소프트웨어"는 뉴클레오티드 또는 아미노산 서열의 분석에 유용한 임의의 컴퓨터 알고리즘 또는 소프트웨어 프로그램을 지칭한다. "서열 분석 소프트웨어"는 상업적으로 이용가능하거나 독립적으로 개발할 수 있다. 전형적인 서열 분석 소프트웨어는, 이로써 한정되는 것은 아니지만, 1.) GCG 프로그램 쉬트(Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, WI); 2.)　BLASTP, BLASTN, BLASTX(Altschul et　al., J.　Mol. Biol., 215:403-410 (1990)); 3.)　DNASTAR(DNASTAR, Inc. Madison, WI); 4.) Sequencher(Gene Codes Corporation, Ann Arbor, MI); 및 5.)　스미스-워터맨(Smith-Waterman) 알고리즘을 도입한 FASTA 프로그램(참조: W. R. Pearson, Comput. Methods Genome Res ., [Proc. Int. Symp.] (1994), Meeting Date 1992, 111-20. Editor(s): Suhai, Sandor. Plenum: New York, NY)을 포함할 것이다. 본원과 관련하여, 서열 분석 소프트웨어가 분석에 사용되는 경우, 당해 분석 결과는, 달리 명시하지 않는 한, 참조된 프로그램의 "디폴트 값"에 기반하는 것으로 이해될 것이다. 본원에 사용된 바와 같이, "디폴트 값"은, 최초로 개시할 때, 당해 소프트웨어에 본래 로딩된 임의 세트의 값 또는 파라미터를 의미할 것이다.
본원에 사용된 표준 재조합 DNA 및 분자 클로닝 기술은 당해 기술분야에 공지되어 있고, 예를 들면, 문헌[참조: Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2000); and by Silhavy et al., Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1984); and by Ausubel et al., Current Protocols in Molecular Biology, published by Greene Publishing Assoc. and Wiley-Interscience (1987 to present)]에 기재되어 있다.
본원에 기재된 재조합 숙주의 유전자 조작은 표준 유전자 기술 및 스크리닝을 사용하여 수행할 수 있고, 유전자 조작에 적합한 임의의 숙주 세포에서 이루어질 수 있다. 일부 양태에서, 본원에 기재된 재조합 숙주 세포는 유전자 변형 및 재조합체 유전자 발현에 유용한 임의의 생물체 또는 미생물 숙주일 수 있다. 일부 양태에서, 재조합체 숙주는, 이로써 한정되는 것은 아니지만, 쌍자엽 식물 및 단자엽 식물 둘 다를 포함하는 고등 식물, 및 작물 식물 및 이들의 오일을 위해 사용된 식물을 포함하는 소비성 식물일 수 있다. 따라서, 임의의 식물 종 또는 식물 세포가 하기 추가로 기재된 바와 같이 선택될 수 있다.
본 발명의 오일은 브라시카 종의 종자 오일에서 DHA 및/또는 EPA를 생산하는 캐놀라 재배종으로부터 수득될 수 있고, 여기서 상기 오일은 70중량% 이상의 올레산(C18:1) 및/또는 4중량% 이하의 리놀렌산(C18:3)을 포함하는 지방산 함량을 갖는다. 이러한 오일은 심장에 유익하고, 요식업 및 소비자 팩키징된 상품 적용을 위한 증가된 안정성을 갖는다. 이러한 오일은 또한 수소화 필요성을 감소시키고, 식품 산업에서 사용된 대두, 팜 및 다수의 기타 오일과 비교하여 영양상 잇점을 제공한다. 이러한 오일의 산화 안정성은 당해 기술분야에 공지된 산화방지제 및 가공 첨가제의 첨가에 의해 추가로 증가될 수 있다.
본 발명의 오일은 또한 비요식업 또는 식이 공정 및 조성물에 사용될 수 있다. 일부 이들 용도는 산업, 화장 및 의약일 수 있다. 본 발명의 오일은 또한 본 발명의 오일이 적합한 임의의 적용에 사용될 수 있다. 일반적으로, 본 발명의 오일은, 예를 들면, 다양한 분야에서 무기질 오일, 에스테르, 지방산 또는 동물 지방, 예를 들면, 윤활제, 윤활 첨가제, 금속 작동 유체, 유압 유체 및 내화성 유압 유체를 대체하기 위해 사용될 수 있다. 본 발명의 오일은 또한 개질유를 생산하는 공정에서 재료로서 사용될 수 있다. 본 발명의 오일을 개질시키는 기술의 예는 분별화, 수소화, 오일의 올레산 또는 리놀렌산 함량의 변화, 및 당해 기술분야의 통상의 지식을 가진 자에게 공지된 기타 개질 기술을 포함한다.
본 발명의 오일에 대한 화장 용도의 예는 화장 조성물에서 연화제; 석유 젤리 대체; 비누의 구성 부분, 또는 비누 제조 공정에서의 재료; 경구 치료 용액의 구성 부분; 에이징(ageing) 처리 조성물의 구성 부분; 및 피부 및 모발 에어로졸 폼 제제의 구성 부분으로서의 용도를 포함한다.
추가로, 본 발명의 오일은 의학적 용도에 사용될 수 있다. 예를 들면, 본 발명의 오일은 감염에 대한 보호벽에서 사용될 수 있고, 오메가-9 지방산이 높은 오일은 이식 그래프트(graft) 생존을 향상시키기 위해 사용될 수 있다(미국 특허 제6,210,700호).
상기는 본 발명의 오일이 적합한 비-요리(non-culinary) 용도의 비제한적 예임을 이해해야 한다. 전술한 바와 같이, 본 발명의 오일 및 개질유는 당해 기술분야의 통상의 지식을 가진 자에게 공지된 모든 적용에서, 예를 들면, 무기유, 에스테르, 지방산 또는 동물 지방을 대체하기 위해 사용될 수 있다.
PUFA 신타제 시스템
진핵 생물체에서 장쇄 PUFA(LC-PUFA)를 합성하는 "표준" 또는 "전통적" 경로는 중간쇄 길이의 포화 또는 모노불포화된 지방산의 신장 및 불포화를 수반하고, 이는 기재되어 있다. PUFA 신타제 시스템을 통한 장쇄 PUFA를 합성하는 경로는 또한 기재되어 있고, "표준" 경로와 매우 상이하다. 구체적으로, PUFA 신타제는 탄소원으로서 말로닐-CoA를 사용하고, 임의의 상당량으로 중간체를 방출하지 않고서 최종 PUFA를 생산한다. 또한, PUFA 신타제에는, 적절한 cis 이중결합이 산소를 필요로 하지 않는 메카니즘을 사용하여 합성 동안 부가된다. 일부 양태에서, NADPH가 합성 사이클 동안 환원제로서 사용된다.
본 발명은, (내생적 또는 유전자 조작에 의해) PUFA 신타제 시스템을 발현시키기 위해 유전자 변형된 숙주 생물체(예: 식물)에 관한 것이다. 일부 양태에서, PUFA 신타제 시스템을 발현시키기 위해 유전자 변형된 생물체(여기서, 당해 생물체는 이러한 시스템을 자연적으로 (내생적, 유전자 변형 없이) 발현하지 않거나, 당해 생물체가 유전적으로 변형되는 적어도 특정 PUFA 신타제 또는 이의 부분을 자연적으로 발현하지 않는다)는 PUFA 신타제 또는 당해 생물체에 의해 내생적으로 발현되지 않는 또 다른 단백질을 사용한 당해 생물체의 변형과 관련하여 본원에서 "이종성" 숙주 생물체로 지칭될 수 있다. 본 발명의 유전자 변형은 PUFA 신타제 시스템을 내생적으로 발현시키는 숙주 생물체에서 PUFA 생산을 개선시키기 위해 사용될 수 있고, 여기서 생물체는 상이한 PUFA 신타제 또는 이의 부분으로 추가로 변형되지 않는다.
본 발명에 따르는 PUFA 신타제 시스템은, 선택된 사이클에서 트랜스-시스 이성화 및 에노일 환원 반응을 포함하는 비반복성 공정 뿐만 아니라 지방산 쇄의 반복성 공정 둘 다를 수행하기 위해 함께 작용할 수 있는 몇몇 다작용성 단백질을 포함할 수 있다(특히 해양 박테리아로부터 PUFA 신타제 시스템을 단일 작용 단백질을 포함할 수 있다). 이들 단백질은 또한 본원에서 코어 PUFA 신타제 효소 복합체 또는 코어 PUFA 신타제 시스템으로서 지칭될 수 있다. 이들 단백질에 함유된 도메인 및 모티프의 일반 작용은 당해 기술분야에 개별적으로 공지되어 있고, 해양 박테리아 및 진핵 생물체로부터 다양한 PUFA 신타제 시스템과 관련하여 상세히 기재되어 있다[참조: 미국특허 제6,140,486호; 미국특허 제6,566,583호; Metz et al., Science 293:290-293 (2001); 미국 특허출원 공개번호 2002/0194641; 미국 특허출원 공개번호 2004/0235127; 미국 특허출원 공개번호 2005/0100995, 및 WO 2006/135866]. 당해 도메인은, 상기 언급한 바와 같이, 단일 단백질(예: 당해 도메인 및 단백질은 동의어임)로서 또는 단일 단백질 중의 2개 이상(다중) 도메인 중의 하나로서 발견될 수 있다. 해양 박테리아 및 트라우스토키트리움(Thraustochytrium) 구성원으로부터 다양한 PUFA 신타제의 도메인 구축 및 이러한 PUFA 신타제를 포함하는 유전자 및 단백질의 구조적 및 작용적 특징은 문헌[참조: 미국특허 제6,140,486호; 미국특허 제6,566,583호; Metz et al., Science 293:290-293 (2001); 미국 특허출원 공개번호 2002/0194641; 미국 특허출원 공개번호 2004/0235127; 미국 특허출원 공개번호 2005/0100995 및 WO 2006/135866]에 기재되어 있다.
PUFA 신타제 활성을 갖는 폴리뉴클레오티드, 유전자 및 폴리펩티드의 다수의 예는 당해 기술분야에 공지되어 있고, 본원에 기재된 유전자 변형된 숙주에서 사용될 수 있다. 본 발명에 유용한 PUFA 신타제 단백질 또는 도메인은 박테리아 및 비박테리아 PUFA 신타제 둘 다를 포함할 수 있다. 비박테리아 PUFA 신타제는 진핵생물과 같이 박테리아가 아닌 생물체로부터 기원하거나 유래되는 시스템이다. 박테리아 PUFA 신타제는, 예를 들면, 미국 특허 공개공보 제2008/0050505호에 기재되어 있다. 다른 PKS 시스템(유형 I 반복 또는 표준, 유형 II 또는 유형 III) 또는 FAS 시스템으로부터의 PUFA 신타제 작용성 도메인 또는 단백질 뿐만 아니라 박테리아 PUFA 신타제 작용성 도메인과 함께 비박테리아 PUFA 신타제 작용성 도메인을 도입한 본 발명의 유전자 변형된 식물이 생산될 수 있다.
일부 양태에서, 본 발명의 PUFA 신타제 시스템은 3개 이상의 단백질 상에 통상 함유되는 적어도 하기 생물학적 활성 도메인: (a) 적어도 하나의 에노일-ACP 리덕타제(ER) 도메인; (b) 다중 아실 캐리어 단백질(ACP) 도메인(예: 적어도 1 내지 4개, 및 바람직하게는 적어도 5개의 ACP 도메인, 및 일부 양태에서 6, 7, 8, 9, 10개, 또는 10개까지의 ACP 도메인); (c) 적어도 2개의 β-케토아실-ACP 신타제(KS) 도메인; (d) 적어도 하나의 아실트랜스퍼라제(AT) 도메인; (e) 적어도 하나의 β-케토아실-ACP 리덕타제(KR) 도메인; (f) 적어도 2개의 FabA-형 β-하이드록시아실-ACP 데하이드라제(DH) 도메인; (g) 적어도 하나의 쇄 길이 인자(CLF) 도메인; (h) 적어도 하나의 말로닐-CoA:ACP 아실트랜스퍼라제(MAT) 도메인을 포함한다. 일부 양태에서, 본 발명에 따르는 PUFA 신타제 시스템은 또한 데하이드라타제(DH) 보존된 활성 부위 모티브를 함유하는 적어도 하나의 영역을 포함한다.
일부 양태에서, PUFA 신타제 시스템은 적어도 하기 생물학적 활성 도메인: (a) 적어도 하나의 에노일-ACP 리덕타제(ER) 도메인; (b) 적어도 5개의 아실 캐리어 단백질(ACP) 도메인; (c) 적어도 2개의 β-케토아실-ACP 신타제(KS) 도메인; (d) 적어도 하나의 아실트랜스퍼라제(AT) 도메인; (e) 적어도 하나의 β-케토아실-ACP 리덕타제(KR) 도메인; (f) 적어도 2개의 FabA-형 β-하이드록시아실-ACP 데하이드라제(DH) 도메인; (g) 적어도 하나의 쇄 길이 인자(CLF) 도메인; 및 (h) 적어도 하나의 말로닐-CoA:ACP 아실트랜스퍼라제(MAT) 도메인을 포함한다. 일부 양태에서, 본 발명에 따르는 PUFA 신타제 시스템은 또한, FabA-형 DH 도메인의 일부가 아닌 데하이드라타제(DH) 보존된 활성 부위 모티브를 함유하는 적어도 하나의 영역 또는 도메인을 포함한다. 이들 도메인 각각의 구조적 및 작용적 특징은 문헌[참조: 미국 특허출원 공개번호 2002/0194641; 미국 특허출원 공개번호 2004/0235127; 미국 특허출원 공개번호 2005/0100995; 미국 특허출원 공개번호 2007/0245431 및 WO 2006/135866]에 상세히 기재되어 있다.
코어 스키조키트리움 PUFA 신타제 시스템을 형성하는 3개의 개방 판독 프레임(open reading frame)이 있고, 이는 상기 기재되어 있다[참조: 미국 특허출원 공개번호 2007/0245431]. 각 개방 판독 프레임의 도메인 구조는 다음과 같다.
스키조키트리움 개방 판독 프레임 A(OrfA 또는 Pfa1): OrfA는 2910 아미노산 서열을 인코딩하는 8730 뉴클레오티드 서열(정지 코돈을 포함하지 않음)이다. OrfA 내에는 12개 도메인, (a) 하나의 β-케토 아실-ACP 신타제(KS) 도메인; (b) 하나의 말로닐-CoA:ACP 아실트랜스퍼라제(MAT) 도메인; (c) 9개의 아실 캐리어 단백질(ACP) 도메인; 및 (d) 하나의 케토리덕타제(KR) 도메인이 있다. 스키조키트리움 종 ATCC 20888, 및 ATCC 20888의 딸 균주(스키조키트리움 종 균주 N230D로 표시됨) 둘 다로부터 OrfA를 인코딩하는 게놈 DNA 클론(플라스미드)가 분리되고 서열분석되었다.
게놈 클론 pJK1126(pJK1126 OrfA 게놈 클론으로 표시됨, 스키조키트리움 ATCC 20888로부터 "OrfA" 유전자를 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, ATCC 수탁번호 PTA-7648로 지정되었다.
게놈 클론 pJK306(pJK306 OrfA 게놈 클론으로 표시됨, 스키조키트리움 종 N230D(pJK320와 2.2kB 중첩)로부터 OrfA 유전자의 5' 부분을 함유하는 이. 콜라이 플라스미드 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, ATCC 수탁번호 PTA-7641로 지정되었다.
게놈 클론 pJK320(pJK320 OrfA 게놈 클론으로 표시됨, 스키조키트리움 종 N230D(pJK306과 2.2kB 중첩)로부터 OrfA 유전자의 3' 부분을 함유하는 이. 콜라이 플라스미드 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, ATCC 수탁번호 PTA-7644로 지정되었다.
스키조키트리움 개방 판독 프레임 B(OrfB or Pfa2): OrfB는 2059 아미노산 서열을 인코딩하는 6177 뉴클레오티드 서열(정지 코돈을 포함하지 않음)이다. OrfB에는 4개 도메인: (a) 하나의 케토 아실-ACP 신타제(KS) 도메인; (b) 하나의 쇄 길이 인자(CLF) 도메인; (c) 하나의 아실 트랜스퍼라제(AT) 도메인; 및 (d) 하나의 에노일 ACP-리덕타제(ER) 도메인이 있다. 스키조키트리움 종 ATCC 20888, 및 ATCC 20888의 딸 균주(스키조키트리움 종 균주 N230D로서 표시됨) 둘 다로부터 OrfB를 인코딩하는 게놈 DNA 클론(플라스미드)는 분리되고 서열분석되었다.
게놈 클론 pJK1129(pJK1129 OrfB 게놈 클론으로 표시됨, 스키토키트리움 종 ATCC 20888로부터 "OrfB" 유전자를 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, 수탁번호 PTA-7649로 지정되었다.
게놈 클론 pJK324(pJK324 OrfB 게놈 클론으로 표시됨, 스키조키트리움 종 N230D로부터 OrfB 유전자 서열을 함유하는 이. 콜라이 플라스미드 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, ATCC 수탁번호 PTA-7643로 지정되었다.
스키조키트리움 개방 판독 프레임 C(OrfC 또는 Pfa3): OrfC는 1502 아미노산 서열을 인코딩하는 4506 뉴클레오티드 서열(정지 코돈을 포함하지 않음)이다. OrfC에는 3개 도메인: (a) 2개의 FabA-형 하이드록시 아실-ACP 데하이드라제(DH) 도메인; 및 (b) 하나의 에노일 ACP-리덕타제(ER) 도메인이 있다. 스키조키트리움 종 ATCC 20888, 및 ATCC 20888의 딸 균주(스키조키트리움 종 균주 N230D로 표시됨)은 분리되고 서열분석되었다.
게놈 클론 pJK1131(pJK1131 OrfC 게놈 클론으로 표시됨, 스키조키트리움 ATCC 20888로부터 "OrfC" 유전자를 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, ATCC 수탁번호 PTA-7650로 지정되었다.
게놈 클론 pBR002(pBR002 OrfC 게놈 클론으로 표시됨, 스키조키트리움 종 N230D로부터 OrfC 유전자 서열을 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2006년 6월 8일자로 기탁되었고, ATCC 수탁번호 PTA-7642로 지정되었다.
또한, 상기한 코어 트라우토키트리움 PUFA 신타제를 형성하는 3개의 개방 판독 프레임이 있다. 각각의 개방 판독 프레임의 도메인 구조는 다음과 같다.
트라우스토키트리움 23B 개방 판독 프레임 A(OrfA): OrfA는 2811 아미노산 서열을 인코딩하는 8433 뉴클레오티드 서열(정지 코돈을 포함하지 않음)이다. 하기 도메인: (a) 하나의 β-케토아실-ACP 신타제(KS) 도메인; (b) 하나의 말로닐-CoA:ACP 아실트랜스퍼라제(MAT) 도메인; (c) 8개의 아실 캐리어 단백질(ACP) 도메인; 및 (d) 하나의 β-케토아실-ACP 리덕타제(KR) 도메인이 Th. 23B OrfA에 존재한다.
게놈 클론 Th23BOrfA_pBR812.1(Th23BOrfA_pBR812.1 게놈 클론으로 표시됨, 트라우스토키트리움 23B로부터 OrfA 유전자 서열을 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), University Boulevard, Manassas, Va. 20110-2209 USA]에 2007년 3월 1일자로 기탁되었고, ATCC 수탁번호 PTA-8232로 지정되었다. 게놈 클론 Th23BOrfA_pBR811(Th23BOrfA_pBR811 게놈 클론으로 표시됨, 트라우스토키트리움 23B로부터 OrfA 유전자 서열을 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2007년 3월 1일자로 기탁되었고, ATCC 수탁번호 PTA-8231로 지정되었다.
트라우스토키트리움 23B 개방 판독 프레임 B(OrfB): OrfB는 1935 아미노산 서열을 인코딩하는 5805 뉴클레오티드 서열(정지 코돈을 포함하지 않음)이다. 하기 도메인: (a) 하나의 β-케토아실-ACP 신타제(KS) 도메인; (b) 하나의 쇄 길이 인자(CLF) 도메인; (c) 하나의 아실트랜스퍼라제(AT) 도메인; 및 (d) 하나의 에노일-ACP 리덕타제(ER) 도메인이 Th. 23B OrfB에 존재한다. 게놈 클론 Th23BOrfB_pBR800(Th23BOrfB_pBR800 게놈 클론으로 표시됨, 트라우스토키트리움 23B로부터 OrfB 유전자 서열을 함유하는 이. 콜라이 플라스미드 벡터 형태)은 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2007년 3월 1일자로 기탁되었고, ATCC 수탁번호 PTA-8227로 지정되었다.
트라우키트리움 23B 개방 판독 프레임 C(OrfC): OrfC는 1470 아미노산 서열을 인코딩하는 4410 뉴클레오티드 서열(정지 코돈을 포함하지 않음)이다. 하기 도메인: (a) FabA 단백질(트랜스-2-데세노일-ACP의 합성 및 시스-데세노일-ACP에 대한 이 생성물의 가역적 이성화를 촉매하는 효소)에 대해 상동성을 갖는 2개의 FabA-형 β-하이드록시아실-ACP 데하이드라제(DH) 도메인; 및 (b) 스키조키트리움 OrfB의 ER 도메인에 대해 높은 상동성을 갖는 하나의 에노일-ACP 리덕타제(ER) 도메인이 Th. 23B OrfC에 존재한다. 게놈 클론 Th23BOrfC_pBR709A(Th23BOrfC_pBR709A 게놈 클론으로 표시됨, 트라우스토키트리움 23B로부터 OrfC 유전자 서열을 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2007년 3월 1일자로 기탁되었고, ATCC 수탁번호 PTA-8228로 지정되었다.
키메라 또는 하이브리드 PUFA 신타제 시스템: 일부 양태에서, PUFA 신타제 시스템은 본원에 기재된 임의의 것들로부터 선택된 도메인을 포함하고, 여기서 당해 도메인은 본원에 기재된 최소 요건에 부합하는 완전 PUFA 신타제 시스템을 형성하기 위해 조합된다(예: 혼합 및 합치됨). 일부 양태에서, 본 발명의 유전자 변형된 생물체는 또 다른 PUFA 신타제 시스템의 적어도 하나의 도메인 또는 이의 생물학적 활성 단편으로 추가로 변형시킬 수 있다. 일부 양태에서, PUFA 신타제 시스템의 임의의 도메인은 PUFA 신타제 시스템에서 당해 도메인의 작용을 변형 또는 향상시키기 위해(예: 당해 시스템에 의해 생산된 PUFA 타입 또는 이의 비율을 변형시키기 위해) 이들의 자연 구조로부터 변형될 수 있다. 키메라 PUFA 신타제 시스템을 생산하기 위한 도메인의 이러한 혼합은 본원에 참조된 특허 및 공보에 기재되어 있다.
일부 양태에서, PUFA 신타제 시스템은 스키조키트리움(Schizochytrium) PUFA 신타제 시스템을 포함하고, 여기서 스키조키트리움 PUFA 신타제 시스템의 OrfC는 트라우스토키트리움 23B의 OrfC로 대체된다. 일부 양태에서, 트라우스토키트리움 23B의 이러한 키메라 OrfC는 스키조키트리움 코돈 사용을 위해 최적화된 핵산 서열에 의해 인코딩된다. 이러한 키메라 OrfC의 비제한적인 예로서, 플라스미드 pThOrfC-synPS(pThOrfC-synPS로 표시됨, 스키조키트리움 또는 기타 이종성 숙주에서 발현시키기 위해 최적화된 "완전 스티치" 합성 트라우스토키트리움 23B PUFA PKS OrfC 코돈을 함유하는 이. 콜라이 플라스미드 벡터 형태)는 아메리칸 타입 컬쳐 컬렉션[American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 USA]에 2007년 3월 1일자로 기탁되었고, ATCC 수탁번호 PTA-8229로 지정되었다(참조: 미국 특허출원 공개번호 2008/0022422).
본 발명의 유전자 변형된 생물체에 사용될 수 있는 PUFA 신타제 유전자 및 폴리펩티드의 다른 예는, 이로써 한정되는 것은 아니지만, 본원에 추가로 기재된 방법에 의해 형성된 하기 코돈-최적화된 서열: 서열번호 1(SzPUFA OrfA v3 단백질); 서열번호 2(SzPUFA OrfB v3 단백질); 서열번호 3(hSzThPUFA OrfC v3 단백질); 서열번호 6(SzPUFA OrfA 유전자); 서열번호 7(SzPUFA OrfB v3 유전자); 및 서열번호 8(hSzThPUFA OrfC v3 유전자), 및 이러한 서열의 활성 변이체, 부분, 단편 또는 유도체를 포함하고, 여기서, 이러한 유전자는 PUFA 신타제 활성을 인코딩하거나 이러한 폴리펩티드 또는 단백질은 PUFA 신타제 활성을 갖는다. 본 발명은 하나 이상의 이러한 서열을 포함하거나 이들로 이루어진 분리된 폴리뉴클레오티드 또는 폴리펩티드를 포함한다.
본 발명의 유전자 변형된 생물체에 사용될 수 있는 PUFA 신타제 유전자 및 폴리펩티드의 다른 예는, 이로써 한정되는 것은 아니지만, 본원에 기재된 PUFA 신타제 또는 서열 중의 어느 하나와 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% 또는 100% 서열 동일성을 갖는 PUFA 신타제 유전자 또는 폴리펩티드를 포함한다. 유용한 범위는 임의의 이들 값(예를 들면, 60% 내지 99%, 65% 내지 95%, 70% 내지 95%, 75% 내지 95%, 80% 내지 95%, 85% 내지 95%, 또는 90% 내지 99%) 사이에서 선택될 수 있다. 본 발명의 유전자 변형된 생물체에서 사용될 수 있는 PUFA 신타제 유전자 및 폴리펩티드의 또 다른 예는, 이로써 한정되는 것은 아니지만, 본원에 기재된 PUFA 신타제 또는 서열의 임의의 하나의 활성 변이체, 부분, 유도체의 단편을 포함하고, 여기서 이러한 유전자는 PUFA 신타제 활성을 인코딩하거나 이러한 폴리펩티드는 PUFA 신타제 활성을 갖는다.
일부 양태에서, PUFA 신타제 시스템은 조류 PUFA 신타제일 수 있다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 1의 아미노산 서열과 적어도 60% 내지 90% 동일한 아미노산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 1의 아미노산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 6의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 6의 핵산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 2의 아미노산 서열과 적어도 80% 동일한 아미노산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 2의 아미노산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 7의 핵산 서열과 적어도 80% 동일한 핵산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 7의 핵산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 3의 아미노산 서열과 적어도 80% 동일한 아미노산 서열을 포함할 수 있다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 3의 아미노산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 8의 핵산 서열과 적어도 80% 동일한 핵산 서열을 포함한다. 일부 양태에서, PUFA 신타제 시스템을 인코딩하는 핵산 서열은 서열번호 8의 핵산 서열을 포함한다.
일부 양태에서, PUFA 신타제 시스템은 서열번호 1, 2 또는 3의 아미노산 서열 또는 이의 임의의 조합을 포함한다. 일부 양태에서, PUFA 신타제 시스템은 서열번호 6, 7 또는 8의 핵산 서열 또는 이의 임의의 조합을 포함한다.
일부 양태에서, 다른 PUFA 신타제 유전자의 서열 및/또는 폴리펩티드는 본원에 기재되고 당해 기술분야에서 이용가능한 서열을 사용하여 당업자에게 공지된 문헌 및 생물정보 데이터베이스에서 동정될 수 있다. 예를 들면, 이러한 서열은 공지된 PUFA 신타제 유전자 또는 폴리펩티드 서열과 함께 공중 이용가능한 데이터베이스의 BLAST 조사를 통해 동정될 수 있다. 이러한 방법에서, 동정은 단백질 중량 매트릭스의 GAP PENALTY=10, GAP LENGTH PENALTY=0.1 및 Gonnet 250 시리즈의 디폴트 파라미터를 사용하는 클러스털 W 정렬 방법에 기반할 수 있다.
추가로, 본원에 기재되거나 당해 기술분야에 공지된 PUFA 신타제 유전자 또는 폴리펩티드 서열은 자연에서 다른 PUFA 신타제 동족체를 동정하는데 사용될 수 있다. 예를 들면, 본원에 기재된 PUFA 신타제 핵산 단편 각각은 상동성 단백질을 인코딩하는 유전자를 분리하는데 사용될 수 있다. 서열 의존성 프로토콜을 사용한 상동성 유전자의 분리는 당해 기술분야에 공지되어 있다. 서열 의존성 프로토콜의 예는, 이로써 한정되는 것은 아니지만, (1) 핵산 하이브리드화 방법; (2) 핵산 증폭 기술의 다양한 사용에 의해 예시되는 DNA 및 RNA 증폭 방법[참조: 폴리머라제 연쇄 반응(PCR), Mullis et 　 al., U.S. Patent　No. 4,683,202; 리가제 연쇄 반응(LCR), Tabor, S. et 　 al., Proc . Acad . Sci . USA 82:1074 (1985); 또는 본쇄 치환 증폭(SDA), Walker et 　 al., Proc . Natl . Acad . Sci . U.S.A., 89:392 (1992)]; 및 (3) 라이브러리 구성 및 상보성에 의한 스크리닝 방법을 포함한다.
이들 모든 방법은 표적 단백질을 인코딩하는 공지되거나 동정된 서열을 사용하는 당해 기술분야의 통상의 지식을 가진 자에 의해 용이하게 실시될 수 있다. 일부 양태에서, 표적 PUFA 신타제 코딩 서열을 둘러싸는 DNA 서열은 또한 일부 변형 공정에 유용하고, 공중 이용가능한 데이터베이스에서 당해 기술분야의 통상의 지식을 가진 자에 의해 용이하게 발견될 수 있다. 유전자 돌연변이를 생성하는 방법은 당해 기술분야에서 통상적이고 공지되어 있으며, 돌연변이체를 생성하는 활동에 적용될 수 있다.
포스포판테티에닐 트랜스퍼라제
포스포판테티에닐 트랜스퍼라제(PPTase)는 지방산 합성, 폴리케타이드 합성 및 비리보좀 펩티드 합성으로 특징지어지는 효소 부류이다. 특히, PUFA 신타제 효소에 존재하는 ACP 도메인은 조효소 A로부터 아실 캐리어 단백질(ACP)로의 보조인자(4-포스포판테테인)의 부착에 의한 활성화를 필요로 한다. 이러한 보조인자의 부착은 PPTase에 의해 수행된다. 숙주 생물체의 내생적 PPTase는 PUFA 신타제 ACP 도메인을 활성화시킬 수 없고, 따라서 당해 작용을 수행할 수 있는 PPTase를 제공할 필요가 있다. 다수의 PPTase의 서열은 공지되어 있고, 활성에 중요한 아미노산 잔기의 돌연변이 분석[참조: Mofid et al., Biochemistry 43:4128-36 (2004)] 뿐만 아니라 결정 구조가 측정되었다[참조: Reuter et al., EMBO J. 18:6823-31 (1999)].
기질로서 본원에 기재된 OrfA ACP 도메인을 인지하는 것으로 이미 입증된 이종성 PPTase의 한 가지 예는 노스톡 종(Nostoc sp .) PPC 7120(이전에 아나바에나 종(Anabaena sp .) PPC 7120으로 불리움)의 Het I 단백질이다. Het I은 당해 생물체의 이형세포에 존재하는 당-지질 층의 성분인 장쇄 하이드록시-지방산의 합성에 관여하는 것으로 공지된 노스톡 중의 유전자 클러스터에 존재한다[참조: Black and Wolk, J. Bacteriol . 176:2282-2292 (1994); Campbell et al., Arch . Microbiol . 167:251-258 (1997)]. Het I은 당해 클러스터에 존재하는 단백질(Hgl E)의 ACP 도메인를 활성화시키는 것 같다. Hgl E의 2개 ACP 도메인은 스키조키트리움 Orf A 및 기타 PUFA 신타제에서 발견된 ACP 도메인과 고도의 서열 상동성을 갖는다.
일부 양태에서, PUFA 신타제는 적어도 하나의 4'-포스포판테테이닐 트랜스퍼라제(PPTase) 도메인을 포함하는 것으로 간주될 수 있거나, 이러한 도메인은 PUFA 신타제에 대한 보조 도메인 또는 단백질인 것으로 간주될 수 있다. PPTase의 구조적 및 작용적 특성은, 예를 들면, 문헌[참조: 미국 특허출원 공개번호 2002/0194641; 미국 특허출원 공개번호 2004/0235127; 및 미국 특허출원 공개번호 2005/0100995]에 상세히 기재되어 있다.
PPTase 활성을 갖는 유전자 및 폴리펩티드의 다수의 예는 당해 기술분야에 공지되어 있고, 이들이 사용된 특정 PUFA 신타제의 ACP 도메인을 활성화시킬 수 있는 경우, 본 발명의 유전자 변형된 생물체에 사용될 수 있다. 본 발명의 유전자 변형된 생물체에 사용될 수 있는 유전자 및 폴리펩티드의 예는, 이로써 한정되는 것은 아니지만, 본원에 추가로 기재된 하기 코돈-최적화된 서열: 서열번호 5(NoHetI v3 단백질) 및 서열번호 10 (NoHetI v3 유전자)를 포함한다.
본 발명의 유전자 변형된 생물체에 사용될 수 있는 PPTase 유전자 및 폴리펩티드의 다른 예는, 이로써 한정되는 것은 아니지만, 본원에 기재된 PPTase 또는 서열 중의 어느 하나와 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% 또는 100% 서열 동일성을 갖는 PPTase 유전자 또는 폴리펩티드를 포함한다. 유용한 범위는 이들 임의의 값 사이(예를 들면, 60% 내지 99%, 65% 내지 95%, 70% 내지 95%, 75% 내지 95%, 80% 내지 95%, 85% 내지 95%, 90% 내지 99%)에서 선택될 수 있다. 본 발명의 유전자 변형된 생물체에 사용될 수 있는 PPTase 유전자의 다른 예는, 이로써 한정되는 것은 아니지만, 이러한 유전자가 PPTase 활성을 인코딩하거나 이러한 폴리펩티드가 PPTase 활성을 갖는, 본원에 기재된 PPTase 서열 중의 어느 하나의 활성 변이체, 단편, 부분 또는 유도체를 포함한다.
일부 양태에서, PPTase는 조류 PPTase일 수 있다. 일부 양태에서, PPTase는 서열번호 5의 아미노산 서열과 적어도 60% 내지 99% 동일한 아미노산 서열을 포함할 수 있다. 일부 양태에서, PPTase는 서열번호 5의 아미노산 서열을 포함할 수 있다. 일부 양태에서, 조류 PPTase를 인코딩하는 핵산 서열은 서열번호 10의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함할 수 있다. 일부 양태에서, 조류 PPTase를 인코딩하는 핵산 서열은 서열번호 10의 핵산 서열을 포함할 수 있다.
본 발명의 일부 양태에서, PPTase는 이종성 숙주에서 PPTase의 생산 및/또는 축적을 위해 제공될 수 있다.
일부 양태에서, PPTase를 인코딩하는 유전자 및/또는 폴리펩티드는 또 다른 PPTase 유전자 및/또는 폴리펩티드 서열을 동정하는데 사용될 수 있고/있거나, 다른 세포에서 PPTase 동족체를 동정하는데 사용될 수 있다. 이러한 PPTase 코딩 서열은, 예를 들면, 문헌 및/또는 당해 기술분야의 통상의 지식을 가진 자에게 공지된 생물정보 데이터베이스에 동정될 수 있다. 예를 들면, 생물정보를 사용하여 또 다른 세포 유형에서 PPTase 코딩 서열의 동정은 본원에 기재된 것들과 같이 공지된 PPTase 코딩 DNA 및 폴리펩티드 서열과 함께 공중 이용가능한 데이터베이스의 BLAST(상기 기재된 바와 같음) 조사를 통해 달성할 수 있다. 동정은 단백질 중량 매트릭스의 GAP PENALTY=10, GAP LENGTH PENALTY=0.1 및 Gonnet 250 시리즈의 디폴트 값을 사용하는 블러스탈 W 정렬 방법에 기반한다.
일부 양태에서, 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 부분은 단일 재조합 발현 벡터에 핵산 서열(i) 및 (ii)를 함유한다.
아실- CoA 신타제
본 발명은 장쇄 PUFA 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신세타제(ACoAS) 단백질을 제공한다. PUFA 신타제에 의한 PUFA의 내생적 생산자(스키조키트리움)는 이의 PUFA 신타제의 유리 지방산 생성물을 아실-CoA로 전환시킬 수 있는 하나 이상의 ACoAS를 보유한다. 이는 고도의 PUFA가 이러한 생물체에서 이들 분획을 축적한다는 사실을 입증한다. 따라서, PUFA 신타제를 내생적으로 함유하는 다른 생물체(예: 다른 트라우스토키트리드) 또는 PUFA FFA를 아실-CoAs로 전환시킬 수 있는 다른 생물체(예: 탈라시오시라 슈도나나(Thalassiosira pseudonana) 또는 크립트헤코디니움 코흐니(Crypthecodinium cohnii)) 뿐만 아니라 스키조키트리움은 이종성 숙주에서 발현된 PUFA 신타제의 생성물을 허용 또는 축적시키는데 유용한 효소를 인코딩하는 유전자 공급원을 나타낼 수 있다. 다른 ACoAS 서열은 미국 특허출원 공개번호 2007/0245431에 기재되어 있다.
AcoAS 활성을 갖는 유전자 및 폴리펩티드의 다수의 예는 당해 기술분야에 공지되어 있고, 본 발명의 유전자 변형된 생물체에서 사용될 수 있다. 본 발명의 유전자 변형된 생물체에서 사용될 수 있는 유전자 및 폴리펩티드의 예는, 이로써 한정되는 것은 아니지만, 본원에 추가로 기재된 하기 코돈-최적화된 서열: 서열번호 4(SzACS-2 v3 단백질) 및 서열번호 9(hSzThACS-2 v3 유전자)를 포함할 수 있다.
본 발명의 유전자 변형된 생물체에 사용될 수 있는 ACoAS 유전자 및 폴리펩티드의 다른 예는, 이로써 한정되는 것은 아니지만, 본원에 기재된 ACoAS 또는 서열 중의 어느 하나와 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% 또는 100% 서열 동일성을 갖는 ACoAS 유전자 또는 폴리펩티드를 포함한다. 유용한 범위는 이들 임의의 값 사이(예를 들면, 60% 내지 99%, 65% 내지 95%, 70% 내지 95%, 75% 내지 95%, 80% 내지 95%, 85% 내지 95%, 90% 내지 99%)일 수 있다. 본 발명의 유전자 변형된 생물체에 사용될 수 있는 ACoAS 유전자 및 폴리펩티드의 다른 예는, 이로써 한정되는 것은 아니지만, 유전자가 ACoAS 활성을 인코딩하거나 이러한 폴리펩티드가 ACoAS 활성을 갖는, 본원에 기재된 ACoAS 서열의 어느 하나의 활성 변이체, 단편, 부분 또는 유도체를 포함한다.
일부 양태에서, ACoAS는 조류 ACoAS일 수 있다. 일부 양태에서, ACoAS는 서열번호 4의 아미노산 서열과 적어도 60% 내지 99% 동일한 아미노산 서열을 포함한다. 일부 양태에서, ACoAS는 서열번호 4의 아미노산 서열을 포함할 수 있다. 일부 양태에서, 조류 ACoAS를 인코딩하는 핵산 서열은 서열번호 9의 핵산 서열과 적어도 60% 내지 99% 동일한 핵산 서열을 포함할 수 있다. 일부 양태에서, 조류 ACoAS를 인코딩하는 핵산 서열은 서열번호 9의 핵산 서열을 포함할 수 있다. 일부 양태에서, ACoAS를 인코딩하는 핵산 서열은 서열번호 34의 핵산 서열을 포함한다.
본 발명의 일부 양태에서, ACoAS는 내생적 숙주에서 ACoAS의 개선된 생산 및/또는 축적 뿐만 아니라 이종성 숙주에서 ACoAS의 생산 및/또는 축적을 위해 제공될 수 있다.
일부 양태에서, ACoAS를 인코딩하는 유전자 및/또는 폴리펩티드는 또 다른 ACoAS 유전자 및/또는 폴리펩티드 서열을 동정하는데 사용될 수 있고/있거나, 다른 세포에서 ACoAS 동족체를 동정하는데 사용될 수 있다. 이러한 ACoAS 코딩 서열은, 예를 들면, 당해 기술분야의 통상의 지식을 가진 자에게 공지된 문헌 및/또는 생물정보 데이터베이스에서 동정될 수 있다. 예들 들면, 생물정보를 사용하여 또 다른 세포 유형에서 ACoAS 코딩 서열의 동정은, 본원에 기재된 것들과 같이, 공지된 ACoAS 코딩 DNA 및 폴리펩티드 서열과 함께 공중 이용가능한 데이터베이스의 BLAST(상기 기재한 바와 같음) 조사를 통해 달성될 수 있다. 동정은 단백질 중량 매트릭스의 GAP PENALTY=10, GAP LENGTH PENALTY-0.1 및 Gonnet 250 시리즈의 디폴트 파라미터를 사용하는 클러스털 W 정렬 방법에 기반한다.
일부 양태에서, 유전자 변형된 식물(예: 브라시카), 이의 후대, 세포, 조직 또는 일부는 단일 재조합 발현 벡터에 함유된 핵산 서열(i), (ii) 또는 (iii) 또는 이의 임의의 조합을 포함한다. 일부 양태에서, 핵산 서열(i), (ii) 또는 (iii) 또는 이의 임의의 조합은 하나 이상의 종자 특이적 프로모터의 조절하에 있고/있거나 단일 재조합 발현 벡터에 함유되어 있다.
유전자 변형된 생물체의 제조방법
하나 이상의 목적하는 다중 불포화 지방산을 상당한 고수율로 생산하기 위해, 생물체(예: 식물)은 PUFA 신타제를 식물 내로 도입하기 위해 유전자 변형될 수 있다. 본 발명은 또한 이러한 유전자 변형의 효능을 개선 또는 향상시키고 특히 PUFA 신타제(예: PUFA)의 생산 및/또는 축적을 개선 또는 향상시키는 방법에 관한 것이다.
유전자 변형된 생물체(이로써 한정되지 않지만, 식물 포함)에서 유전자 발현 방법은 당해 기술분야에 공지되어 있다. 일부 양태에서, 발현되는 PUFA 신타제 유전자에 대한 코딩 영역은 하기에 기재된 바와 같은 표적 숙주 세포에 대해 코돈 최적화될 수 있다. 재조합 숙주 세포(이로써 한정되지 않지만, 식물 세포 포함)에서 유전자의 발현은 목적하는 코딩 서열 및/또는 전사 터미네이터에 작동적으로 연결된 프로모터를 필요로 할 수 있다. 다수의 프로모터가 유전자용 벡터의 작제에 사용될 수 있고, 여기에는, 이로써 한정되지 않지만, 종자 특이적 프로모터(예: PvDlec2, LfKCS3 및 FAE 1)가 포함된다. 본 발명에 사용될 수 있는 프로모터의 다른 비제한적 예는 WO 1992/18634에 기재된 아실 캐리어 단백질 프로모터; 문헌[참조: Slightom et al. (Proc . Natl . Acad . Sci . U.S.A. 80:1897-1901; 1983); Sengupta-Gopalan et al. (Proc . Nat . Acad . Sci . 82:3320-3324; 1985); van der Geest et al. (Plant Mol . Biol . 33:553-557; 1997), and Bustos et al. (EMBO J. 10:1469-1479; 1991)]에 개시된 파세올루스 불라기스(Phaseolus vulgaris) 베타-파세올린 프로모터 및 절단된 버젼을 포함한다.
본 발명의 일부 양태에서, 재조합 벡터는 선택된 핵산 서열을 조작하고 숙주 세포 내로 이러한 핵산 서열을 도입하는 도구로서 사용되는 조작된(예: 인공 생산된) 핵산 분자이다. 따라서, 재조합 벡터는, 예를 들면, 선택된 핵산 서열을 숙주 세포에서 발현 및/또는 전달하여 재조합 세포를 형성함으로써 선택된 핵산 서열을 클로닝, 서열분석 및/또는 달리는 조작하는데 사용하기에 적합하다. 이러한 벡터는 통상 이종성 핵산 서열, 즉 클로닝 또는 전달되는 핵산 서열과 인접하여 자연적으로 발견되지 않는 핵산 서열을 함유하지만, 당해 벡터는 또한 본 발명의 핵산 분자에 인접하여 자연적으로 발견되거나 본 발명의 핵산 분자의 발현에 유용한 조절 핵산 서열(예: 프로모터, 비해독된 영역)을 함유할 수 있다. 당해 벡터는 원핵생물 또는 진핵생물의 RNA 또는 DNA일 수 있고, 통상 플라스미드이다. 당해 벡터는 염색체외(extrachromosomal) 요소(예: 플라스미드)로서 유지될 수 있거나, 재조합 생물체(예: 미생물 또는 식물)의 염색체 내로 통합될 수 있다. 전체 벡터는 숙주 세포 내에 또는 특정한 조건하에 당해 위치에 잔류할 수 있고, 플라스미드 DNA를 결실시켜 본 발명의 핵산 분자의 뒤에 잔류시킬 수 있다. 통합된 핵산 분자는 염색체 프로모터 조절하에, 천연 또는 플라스미드 프로모터 조절하에 또는 몇몇 프로모터 조절의 조합하에 존재할 수 있다. 핵산 분자의 단일 또는 다중 카피는 염색체 내로 통합될 수 있다. 본 발명의 재조합 벡터는 적어도 하나의 선별가능한 마커를 함유할 수 있다.
일부 양태에서, 본 발명의 재조합 핵산 분자에 사용된 재조합 벡터는 발현 벡터이다. 이러한 양태에서, 생산되는 생성물(예: PUFA 신타제)를 인코딩하는 핵산 서열은 재조합 핵산 분자를 생산하기 위해 재조합 벡터 내로 삽입된다. 생산되는 단백질을 인코딩하는 핵산 서열은, 재조합 숙주 세포 내에서 핵산 서열의 전사 및 해독이 가능한 벡터에서 핵산 서열을 조절 서열과 작동적으로 연결시키는 방식으로 당해 벡터 내로 삽입된다.
다양한 숙주 생물체 및 세포의 형질전환에 유용한 벡터는 통상적이고, 문헌에 개시되어 있다. 전형적으로, 당해 벡터는 목적하는 숙주에서 자가 복제 또는 염색체 통합을 가능하게 하는 선별가능한 마커 및 서열을 함유한다. 또한, 적합한 벡터는 전사 개시 조절 및 전사 종결 조절 영역을 포함하는 프로모터 영역을 포함할 수 있고, 코딩 영역 사이에 DNA 단편을 삽입하여, 삽입된 코딩 영역의 발현을 제공할 수 있다. 조절 영역 둘 다는 형질전환된 숙주 세포에 상동성인 유전자로부터 유래될 수 있지만, 이러한 조절 영역은 또한 생산 숙주로서 선택된 특이적 종에서 기원하지 않는 유전자로부터 유래될 수도 있다.
본 발명은, 단독으로 또는 본원에 기재된 임의의 하나 이상의 방법과 조합[예를 들면, 코돈 최적화, 세포기관 표적화, 말로닐 CoA의 PUFA 신타제 경쟁의 향상(예: FAS의 억제에 의함) 및/또는 하나 이상의 아실트랜스퍼라제 또는 관련 효소의 발현 중의 임의의 1개, 2개, 3개 또는 4개의 조합]하여 사용되는 본원에 기재된 PUFA 신타제 및 외인성 PPTase를 사용하는, 본원에 기재되고 예시된 하나 이상의 아실-CoA 신세타제의 발현을 포함하여 이종성 숙주에서 PUFA 생산 및/또는 축적을 증가시킨다.
본 발명의 일부 양태는 PUFA 신타제 효소(PPTase) 및/또는 하나 이상의 보조 단백질의 발현의 표적화 및/또는 하나 이상의 숙주 생물체에 대한 표적화 유전자 변형에 관한 것이다. 예를 들면, 일부 양태에서, PUFA 신타제 시스템 및 PPTase의 발현은 식물 색소체에 대해 표적화시킬 수 있다. 일부 양태에서, PUFA 신타제 및 PPTase의 발현은 시토졸에 대해 표적화된다. 일부 양태에서, PUFA 신타제 및 PPTase의 발현은 식물의 색소체 및 시토졸 둘 다에 대해 표적화된다. 임의의 이들 양태에서, 다른 표적이 색소체 또는 시토졸에 대해 유도될 수 있다.
일부 양태에서, 아실-CoA 신세타제를 시토졸에서 발현시켜 DHA 및/또는 기타 LC-PUFA 유리 지방산을 아실-CoAs로 전환시키고, 이는 또한 아실트랜스퍼라제에 의해 이용할 수 있다.
한 가지 예시적 색소체 표적화 서열은 브라시카 나푸스(Brassica napus) 아실-ACP 티오에스테라제로부터 유래되고, 미국 특허출원 공개공보 2007/0245431에 기재되어 있다. 다양한 기타 색소체 표적화 서열은 당해 기술분야에 공지되어 있고, 이종성 숙주가 식물 또는 식물 세포이고 색소체에 대한 표적화가 요구되는 양태에서 사용될 수 있다.
본 발명은, 단독으로 또는 본원에 기재된 하나 이상의 방법과 조합[예를 들면, 코돈 최적화, 말로닐 CoA의 PUFA 신타제 경쟁의 향상(예: FAS의 억제에 의함), 하나 이상의 아실-CoA 신세타제의 발현, 및/또는 하나 이상의 아실트랜스퍼라제 또는 관련 효소의 발현 중의 임의의 1개, 2개, 3개 또는 4개의 조합]하여 사용되는 본원에 기재된 PUFA 신타제의 발현 및 외인성 PPTase를 사용하는 생물체 표적화(예: 식물에서 색소체 또는 엽록체에 대해)의 사용을 포함하여 이종성 숙주에서 PUFA 생산 및/또는 축적을 증가시킨다.
색소체 또는 엽록체에 대한 유전자 생성물의 표적화는 다양한 단백질의 아미노산 종결 말단에서 발견되고 성숙 단백질을 제공하는 도입 동안 분리되는 시그널 서열에 의해 조절된다[참조: 엽록체 표적화와 관련하여, Comai et al., J. Biol . Chem. 263:15104-15109 (1988)]. 이들 시그널 서열을 이종성 유전자 생성물과 융합시켜 엽록체 내로 이종성 생성물의 도입을 수행한다[참조: van den Broeck et al. Nature 313:358-363 (1985)]. 적절한 시그널 서열을 인코딩하는 DNA는, 엽록소 국지화되는 것으로 공지된, RUBISCO 단백질, CAB 단백질, EPSP 신타제 효소, GS2 단백질 및 다수의 기타 단백질을 인코딩하는 cDNA로부터 분리될 수 있다.
본 발명의 일부 양태에서, 본 발명에 사용된 단백질의 국지화는, 예를 들면, 색소체 또는 엽록체에 대한 아세포 구획으로 유도된다. 단백질은 이들의 아미노-말단에 엽록체 통과 펩티드(CTP)를 포함시킴으로써 엽록체로 유도된다. 유사하게는, 단백질은 이들의 N-말단에 색소체 운반 또는 신호전달 펩티드를 포함시킴으로써 색소체로 유도된다.
전구체를 엽록체 도입 기구로 유도하는 아미노 말단 엽록체 표적화 펩티드를 함유하는 보다 긴 전구 단백질로서 합성된 자연 발생 엽록체 표적화 단백질은 당해 기술분야에 공지되어 있다. 엽록체 표적화 펩티드는 일반적으로 엽록체 생물체 내에 위치된 특이적 엔도프로테아제에 의해 분리되고, 따라서 당해 전구체로부터 엽록체 주위로 표적화 성분 및 바람직하게는 활성 효소를 방출한다. 식물 세포의 엽록체 또는 색소체에 대해 유전자 또는 유전자 생성물의 표적화를 유도하는데 적합한 펩티드 코딩 서열의 예는 당해 기술분야의 통상의 지식을 가진 자에게 공지된 페투니아 EPSPS CTP, 아라비돕시스 EPSPS CTP2 및 인트론 등을 포함한다. 이러한 표적화 서열은, 발현된 목적 단백질을, 목적하는 표현형 작용에 필요한 세포 프로세스가 농축되어 있는 세포 부분으로 전달함으로써, 발현된 목적 단백질을 가장 효과적으로 작용하는 세포 구조로 전달되도록 한다. 엽록체 표적화 펩티드의 구체적인 예는 당해 기술분야에 공지되어 있고, 아라비돕시스 탈리아나(Arabidopsis thaliana) 리불로스 비스포스페이트 카복실라제 소형 아단위 ats1A 운반 펩티드, 아라비돕시스 탈리아나 EPSPS 운반 펩티드, 및 제아 메이즈(Zea maize) 리불로스 비스포스페이트 카복실라제 소형 아단위 운반 펩티드를 포함한다.
최적화 운반 펩티드는, 예를 들면, 문헌[참조: van den Broeck et al., Nature, 313:358-363 (1985)]에 기재되어 있다. 원핵세포 및 진핵세포 시그널 서열은, 예를 들면, 문헌[참조: Michaelis et al., Ann. Rev. Microbiol. 36:425 (1982)]에 기재되어 있다. 본 발명에서 사용될 수 있는 운반 펩티드의 추가의 예는 엽록체 운반 펩티드를 포함하고, 예를 들면, 문헌[참조: Von Heijne et al., Plant Mol. Biol. Rep. 9:104-126 (1991); Mazur et al., Plant Physiol. 85:1110 (1987); Vorst et al., Gene 65:59 (1988). Chen & Jagendorf (J. Biol. Chem. 268:2363-2367 (1993)]에 기재된 것들은 이종성 변형유전자의 도입을 위한 엽록체 운반 펩티드를 기재하고 있다. 사용된 이러한 펩티드는 니코티아나 플룸바기니폴리아(Nicotiana plumbaginifolia)로부터의 rbsS 유전자의 운반 펩티드이다[참조: Poulsen et al. Mol. Gen. Genet. 205: 193-200 (1986)]. 본원에서 이종성 단백질을 엽록체로 국지화시키도록 작용하는 하나의 CTP는 바르시카 나푸스 아실-ACP 티오에스테라제로부터 유래된다.
유전자를 엽록체 또는 색소체로 국지화시키는 대체 수단은 엽록체 또는 색소체 형질전환을 포함한다. 엽록체 DNA만을 변화시켜, 본원에 포함된 분자를 도입한 재조합 식물을 생산할 수 있다. 엽록체에서 작용하는 프로모터는 당해 기술분야에 공지되어 있다[참조: Hanley-Bowden et al., Trends in Biochemical Sciences 12:67-70 (1987)]. 이종성 DNA가 삽입된 엽록체 함유 세포를 수득하는 방법 및 조성물은, 예를 들면, 문헌[참조: Daniell et al. (U.S. Patent No. 5,693,507) 및 Maliga et al. (U.S. Patent No. 5,451,513)]에 기재되어 있다.
방법의 조합
본 발명에 따라, 하나 이상의 표적 PUFA의 생산 및 축적을 위한 이종성 숙주의 생산에 있어서, 숙주에서 PUFA의 생산 및/또는 축적을 개선시키기 위해 본원에 기재된 하나 이상(임의의 조합)의 방법을 사용할 수 있다. 실제로, 다양한 방법의 조합은 부가적 또는 상승적일 수 있고 하나 이상의 이러한 방법의 부재와 비교하여 PUFA의 개선된 생산 및/또는 축적을 제공할 것으로 예상된다. 실제로, 실시예는 숙주 생물체에서 PUFA의 생산을 위한 예시적 방법을 제공한다.
본원에 기재된 변형의 이들 조합 또는 기타 변형 또는 변형의 조합을 사용한 임의의 식물 또는 식물 세포는 본 발명에 포함된다. 일부 양태에서, 이러한 식물을 추가로 유전자 변형시켜, 숙주에 의한 PUFA(또는 PUFA 신타제의 기타 생활성 생성물)의 생산 및/또는 축적을 개선시키기 위해 본원에 기재된 보조 단백질(예: ACoAS, GPAT, LPAAT, DAGAT 또는 아세틸 CoA 카복실라제(ACCase)]를 발현시킨다. 추가로, 본원에 기재된 임의의 변형 또는 변형의 조합을 사용하는 임의의 숙주 세포 또는 생물체는, 표적 PUFA를 포함하는 종자 또는 오일을 포함하는 이러한 세포 또는 생물체로부터 유도된 임의의 생성물과 같이, 본 발명에 포함된다.
일부 양태에서, 본 발명에 따라 유전자 변형시키기 위한 식물(예: 식물 숙주 세포)은, 이로써 한정되는 것은 아니지만, 쌍자엽 및 단자엽 식물 및 특히 소비성 식물을 포함하는 임의의 고등 식물을 포함하고, 작물 식물 및 특히 이들의 오일을 위해 사용된 식물을 포함한다. 이러한 식물은, 이로써 한정되는 것은 아니지만, 캐놀라, 대두, 평지씨, 아마인, 옥수수, 홍화, 해바라기 및 담배를 포함한다. 따라서, 임의의 식물 종 또는 식물 세포가 선택될 수 있다. 당해 양태에서, 본원에 사용된 식물 세포 또는 이로부터 성장하거나 유도된 식물은, 이로써 한정되는 것은 아니지만, 캐놀라(Brassica napus); 유채(oilseed rape)(Brassica napus); 인디안 머스타드(Brassica juncea); 에티오피안 머스타드(Brassica carinata); 순무(Brassica rapa); 양배추(Brassica oleracea); 대두(Glycine max); 아마인/아마(Linum usitatissimum); 옥수수(corn)(Zea mays); 홍화(Carthamus tinctorius); 해바라기(Helianthus annuus); 담배(Nicotiana tabacum); 아라비돕시스 탈리아나(Arabidopsis thaliana), 브라질 너트(Betholettia excelsa); 아주까리콩(Ricinus communis); 코코넛(Cocus nucifera); 고수풀(Coriandrum sativum); 목면(Gossypium spp.); 땅콩(Arachis hypogaea); 조조바(Simmondsia chinensis); 오일 팜(Elaeis guineeis); 올리브(Olea eurpaea); 벼(Oryza sativa); 호박(squash)(Cucurbita maxima); 보리(Hordeum vulgare); 밀(Triticum aestivum); 및 좀개구리밥(Lemnaceae sp.)을 포함한다. 일부 양태에서, 식물 종 내의 유전자 배경은 달라질 수 있다.
본원에 사용된 바와 같이 "식물 부분"은 식물의 임의의 부분을 포함하고, 이로써 한정되는 것은 아니지만, 종자(성숙 종자 및 미성숙 종자 포함), 화분, 배아, 꽃, 과실, 발아, 잎, 뿌리, 줄기, 외식편 등을 포함한다. 일부 양태에서, 유전자 변형된 식물은, 목적하는 결과가 달성되도록(예: 증가되거나 변형된 PUFA 신타제 및/또는 PUFA 신타제를 사용한 목적 생성물의 생산 및/또는 축적), 이의 정상(예: 야생형 또는 자연 발생) 형태로부터 변형(예: 돌연변이 또는 변화)된 게놈을 갖는다. 일부 양태에서, 식물의 유전자 변형은 고전적 균주 개발 및/또는 분자 유전자 기술을 사용할 달성할 수 있다. 목적하는 아미노산 서열을 인코딩하는 재조합 핵산 분자가 식물의 게놈 내로 도입된 유전자변형 식물을 생산하는 방법은 당해 기술분야에 공지되어 있다. 일부 양태에서, 본 발명에 따라 유전자 변형시키기 위한 식물은 사람을 포함하여 동물에 의한 소비에 적합한 식물이다.
특히 목적하는 특질, 예를 들면, 내병성, 식물 형질전환 용이성, 오일 함량 또는 프로파일 등에 대해 최적화된 이들 식물로부터의 식물 라인이 생산, 선별 또는 동정될 수 있다. 일부 양태에서, 식물 라인은 식물 육종, 또는 판매자 지원 육종 및 경작 등의 방법을 통해 선별될 수 있다. 일부 양태에서, 식물 세포 배양물이 사용될 수 있고, 이는 예를 들면, 통상의 농업 실시를 사용하여 분화된 식물로 성장하지 않고 경작되지 않지만, 액체 배지에서 유지된다.
일부 양태에서, 식물은 오일 종자 식물일 수 있고, 여기서 오일 종자 및/또는 오일 종자 중의 오일은 PUFA 신타제에 의해 생산된 PUFA를 함유한다. 일부 양태에서, 이러한 오일은, PUFA 신타제의 생성물인, 검출가능한 양의 적어도 하나의 표적 또는 주요 PUFA를 함유한다. 일부 양태에서, 이러한 오일은, 표적 또는 주요 PUFA 생성물이 아니고 야생형 식물에서 내생적 FAS 시스템에 의해 자연적으로 생산되지 않는 중간 또는 부생성물을 실질적으로 포함하지 않을 수 있다(예를 들면, 야생형 식물은 FAS 시스템을 통해 약간 보다 단쇄 또는 중쇄 PUFA, 예를 들면, 18개 탄소 PUFA를 생산하지만, PUFA 신타제 시스템을 사용한 유전자 변형의 결과로서 식물에서 생산된 새로운 또는 추가의 지방산이 존재할 수 있다).
유전자 변형 식물의 생산과 관련하여, 식물의 유전자 조작법은 당해 기술분야에 공지되어 있다. 예를 들면, 식물 형질전환을 위한 다수의 방법이 개발되었고, 이는 단자엽 식물 뿐만 아니라 쌍자엽 식물에 대한 생물학적 및 물리적 형질전환 프로토콜을 포함한다[참조: Goto-Fumiyuki et al., Nature Biotech 17:282-286 (1999); Miki et al., Methods in Plant Molecular Biology and Biotechnology, Glick, B. R. and Thompson, J. E. Eds., CRC Press, Inc., Boca Raton, pp. 67-88 (1993)]. 또한, 식물 세포 또는 조직 형질전환 및 식물의 재생을 위한 벡터 및 시험관내 배양 방법은 이용가능하다[참조예: Gruber et al., Methods in Plant Molecular Biology and Biotechnology, Glick, B. R. and Thompson, J. E. Eds., CRC Press, Inc., Boca Raton, pp. 89-119 (1993)].
본 발명은, 본원에 기재된 이러한 서열의 변형 또는 돌연변이를 포함하는 분리된 핵산 분자 뿐만 아니라 서열번호 6 내지 10으로부터 선택된 핵산 서열을 포함하는 분리된 핵산 분자에 관한 것이다. 본 발명은, 본원에 기재된 이러한 서열의 변형 또는 돌연변이를 포함하는 분리된 폴리펩티드 뿐만 아니라 서열번호 1 내지 5로부터 선택된 아미노산 서열을 포함하는 분리된 폴리펩티드에 관한 것이다.
본 발명은 재조합 발현 벡터 pDAB7361을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7362을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7363을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7365를 포함한다. 본 발명은 재조합 발현 벡터 pDAB7368을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7369를 포함한다. 본 발명은 재조합 발현 벡터 pDAB7370을 포함한다. 본 발명은 재조합 발현 벡터 pDAB100518을 포함한다. 본 발명은 재조합 발현 벡터 pDAB101476을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9166을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9167을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7379을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7380을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9323을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9330을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9337을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9338을 포함한다. 본 발명은 재조합 발현 벡터 pDAB9344를 포함한다. 본 발명은 재조합 발현 벡터 pDAB9396을 포함한다. 본 발명은 재조합 발현 벡터 pDAB101412를 포함한다. 본 발명은 재조합 발현 벡터 pDAB7733을 포함한다. 본 발명은 재조합 발현 벡터 pDAB7734를 포함한다. 본 발명은 재조합 발현 벡터 pDAB101493을 포함한다. 본 발명은 재조합 발현 벡터 pDAB109507을 포함한다. 본 발명은 재조합 발현 벡터 pDAB109508을 포함한다. 본 발명은 재조합 발현 벡터 pDAB109509를 포함한다. 본 발명은 재조합 발현 벡터 pDAB9151을 포함한다. 본 발명은 재조합 발현 벡터 pDAB108207을 포함한다. 본 발명은 재조합 발현 벡터 pDAB108208을 포함한다. 본 발명은 재조합 발현 벡터 pDAB108209를 포함한다. 본 발명은 재조합 발현 벡터 pDAB9159를 포함한다. 본 발명은 재조합 발현 벡터 pDAB9147을 포함한다. 본 발명은 재조합 발현 벡터 pDAB108224를 포함한다. 본 발명은 재조합 발현 벡터 pDAB108225를 포함한다.
본원에 사용된 바와 같이 용어 "형질감염"은 외인성 핵산 분자(예: 재조합 핵산 분자)가 세포 내로 삽입될 수 있는 임의의 방법을 지칭하기 위해 사용된다. 용어 "형질전환"은, 이러한 용어가 조류, 박테리아 및 효모 등의 미생물 세포 내로 또는 식물 세포 내로 핵산 분자의 도입을 지칭하기 위해 사용되는 경우, 용어 "형질감염"과 상호 교대로 사용될 수 있다. 미생물 및 식물 시스템에서, 용어 "형질전환"은 당해 미생물 또는 식물에 의한 외인성 핵산의 인지에 기인하는 유전형질 변화를 기재하기 위해 사용되며, 실질적으로 용어 "형질감염"과 동의어이다. 일부 양태에서, 형질감염 기술은, 이로써 한정되는 것은 아니지만, 형질전환, 입자 충격, 확산, 활성 수송, 배스 초음파처리, 전기천공, 미세주입, 리포펙션, 흡착, 감염 및 원형질 융합을 포함한다.
발현 벡터를 식물로 도입하는 광범위하게 사용된 방법은 아그로박테리움(Agrobacterium)의 자연 형질전환 시스템에 기반한다[참조: Horsch et al., Science 227:1229 (1985)]. 아그로박테리움 투메파시엔스(A. tumefaciens) 및 아그로박테리움 리조게네스(A. rhizogenes)는 식물 세포의 유전자 형질전환에 유용한 것으로 공지된 식물 병원성 토양 박테리아이다. 각각 에이. 투메파시엔스 및 에이. 리조게네스의 Ti 및 Ri 플라스미드는 식물의 유전자 형질전환에 관여하는 유전자를 포함한다[참조: Kado, C. I., Crit. Rev. Plant. Sci. 10:1 (1991)]. 아그로박테리움 벡터 시스템 및 아그로박테리움 매개된 유전자 전이의 기재는 또한, 예를 들면, 문헌[참조: Gruber et al., supra, Miki et al., supra, Moloney et al., Plant Cell Reports 8:238 (1989), 및 미국 특허 제4,940,838호 및 제5,464,763호]에 이용가능하다.
식물 형질전환을 위한 또 다른 공지된 방법은, DNA가 미립의 표면 상에 포함되는 미립 매개된 형질전환이다. 당해 방법에서, 발현 벡터는 식물 세포 벽 및 막을 투과하기에 충분한 속도로 미립을 가속시키는 생물학적 장치에 의해 식물 조직 내로 도입된다[참조: Sanford et al., Part. Sci. Technol. 5:27 (1987), Sanford, J. C., Trends Biotech. 6:299 (1988), Sanford, J. C., Physiol. Plant 79:206 (1990), Klein et al., Biotechnology 10:268 (1992)].
DNA를 식물로 물리적 전달하기 위한 또 다른 방법은 표적 세포의 초음파처리이다[참조: Zhang et al., Bio/Technology 9:996 (1991)]. 또한, 리포좀 또는 구형체 융합은 발현 벡터를 식물 내로 도입하기 위해 사용되어 왔다[참조: Deshayes et al., EMBO J., 4:2731 (1985), Christou et al., Proc Natl. Acad. Sci. USA 84:3962 (1987)]. CaCl2 침전, 폴리비닐 알콜 또는 폴리-L-오르니틴을 사용한 DNA의 원형질체로의 직접 흡수가 또한 보고되어 있다[참조: Hain et al., Mol. Gen. Genet. 199:161 (1985) and Draper et al., Plant Cell Physiol. 23:451 (1982)]. 원형질체 및 전체 세포 및 조직의 전기천공도 또한 기재되어 있다[참조: Donn et al., Abstracts of VIIth International Congress on Plant Cell and Tissue Culture IAPTC, A2-38, p. 53 (1990); D'Halluin et al., Plant Cell 4:1495-1505 (1992) and Spencer et al., Plant Mol. Biol. 24:51-61 (1994)]. 추가로, 실리콘 카바이드 단결정[참조: Kaepler et al., 1990, Plant Cell Reports] 및, 예를 들면, 침지 방법[참조: Clough and Bent, Plant J. 16:735-743 (1998)]를 사용한 식물내 형질전환도 사용될 수 있다. 정확한 식물 형질전환 방법은 형질전환을 위해 선택된 식물 종 및 선택된 식물 세포 유형에 다소 의존하여 달라질 수 있다(예를 들면, 배축 및 자엽 또는 배아 조직 등의 실생(seedling) 유도된 세포 유형).
유전자 작제물을 식물 세포 내로 도입한 후, 식물 세포는 성장시킬 수 있고, 분화 조직, 예를 들면, 발아 및 뿌리가 출아되면 성숙 식물이 생성될 수 있다. 일부 양태에서, 복수의 식물이 생성될 수 있다. 식물을 재생시키는 방법은 당해 기술분야의 통상의 지식을 가진 자에게 공지되어 있고, 예를 들면, 문헌[참조: Plant Cell and Tissue Culture, 1994, Vasil and Thorpe Eds. Kluwer Academic Publishers and in: Plant Cell Culture Protocols (Methods in Molecular Biology 111, 1999 Hall Eds Humana Press)]에서 발견할 수 있다.
일부 양태에서, 본원에 기재된 변형된 식물은 발효 배지에서 배양하거나 토양 등의 적합한 배지에서 성장시킬 수 있다. 일부 양태에서, 고등 식물에 적합한 성장 배지는 식물용의 임의의 성장 배지를 포함할 수 있고, 이는, 이로써 한정되지 않지만, 토양, 모래, 뿌리 성장을 지지하는 임의의 기타 미립자 배지(예: 버미큘라이트, 퍼라이트 등) 또는 수경 재배 뿐만 아니라 고등 식물의 성장을 최적화하는 적합한 광, 물 및 영양 보충물을 포함한다.
재조합 DNA 기술의 사용은, 예를 들면, 숙주 세포에서 핵산 분자의 카피 수를 조작함으로써 형질감염된 핵산 분자의 발현의 조절, 이들 핵산 분자가 전사되는 효율, 생성 전사체가 해독되는 효율, 및 해독후 변형 효율을 개선시킬 수 있음이 당해 기술분야의 통상의 지식을 가진 자에게 이해될 것이다. 추가로, 프로모터 서열을 유전자 조작하여, 천연 프로모터와 비교하여 발현 수준을 개선시킬 수 있다. 핵산 분자의 발현을 조절하는데 유용한 재조합 기술은, 이로써 한정되는 것은 아니지만, 하나 이상의 숙주 세포 염색체 내로 핵산 분자의 통합, 플라스미드에 벡터 안정성 서열의 부가, 전사 조절 시그널의 치환 또는 변형(예: 프로모터, 오퍼레이터, 인핸서), 해독 조절 시그널의 치환 또는 변형(예: 리보솜 결합 부위, 샤인-달가노 서열), 숙주 세포의 코돈 사용에 대응하도록 핵산 분자의 변형, 및 전사체를 불활성화시키는 서열의 결실을 포함한다.
일부 양태에서, 식물은 약제, 향미제, 영양제, 기능성 식품 성분 또는 화장품용 활성제로서 사용된 화합물을 생산하는 식물, 또는 이들 화합물/활성제를 생산하도록 유전자 조작된 식물을 포함할 수 있다.
본 발명의 이들 모든 양태는 본원에 기재된 임의의 유전자 변형된 생물체 및 이러한 생물체를 생산 및 사용하는 방법의 논의에 적용한다.
유전자 변형된 생물체로부터의 생성물
일부 양태에서, 본 발명의 유전자 변형된 생물체는 하나 이상의 다중불포화 지방산(이로써 한정되는 것은 아니지만, EPA(C20:5, n-3), DHA(C22:6, n-3), DPA(C22:5, n-6 또는 n-3), ARA(C20:4, n-6), GLA(C18:3, n-6), ALA(C18:3, n-3) 및/또는 SDA(C18:4, n-3)를 포함함), 및 일부 양태에서, 하나 이상의 보다 장쇄 PUFA(이로써 한정되는 것은 아니지만, EPA(C20:5, n-3), DHA(C22:6, n-3), DPA(C22:5, n-6 또는 n-3) 또는 DTA(C22:4, n-6)를 포함함) 또는 이들의 조합을 생산한다. 일부 양태에서, 본 발명의 유전자 변형된 식물은 하나 이상의 다중불포화 지방산(이로써 한정되는 것은 아니지만, EPA(C20:5, n-3), DHA(C22:6, n-3), 및/또는 DPA(C22:5, n-6 또는 n-3) 또는 이들의 조합을 포함함)을 생산한다.
일부 양태에서, 유전자 변형된 생물체는 본원에 기재된 바와 같이 PUFA 시낱제 시스템 및 PPTase를 재조합에 의해 발현하도록 유전자 변형된 식물이다. 일부 양태에서, 이러한 식물을 추가로 유전자 변형시켜, 숙주에 의한 PUFA(또는 PUFA 신타제의 기타 생활성 생성물)의 생산 및/또는 축적의 개선을 위해 본원에 기재된 바와 같은 보조 단백질(예: ACoAS, GPAT, LPAAT, DAGAT 또는 ACCase)을 발현시킨다.
본 발명의 일부 양태는 목적하는 수의 이중결합과 함께 목적하는 쇄 길이의 다중불포화 지방산의 생산, 및 광범위하게는 이들 PUFA를 포함하는 본원에 기재된 유전자 변형된 식물로부터 수득한 오일(예: 이러한 식물의 오일 또는 종자로부터 수득됨)을 포함한다. 본 발명에 의해 생산될 수 있는 PUFA의 예는, 이로써 한정되는 것은 아니지만, DHA(도코사헥사엔산(C22:6, n-3)), ARA(에이코사테트라엔산 또는 아라키돈산(C20:4, n-6)), DPA(도코사펜타엔산(C22:5, n-6 or n-3)) 및 EPA(에이코사펜타엔산(C20:5, n-3)) 및 이들의 임의의 조합을 포함한다. 본 발명은 PUFA를 생산하는 PUFA 신타제 시스템의 사용을 통해 본 발명자들의 유전자 변형된 식물의 개발에 의해 하나 이상의 목적하는(표적 또는 주요) PUFA가 풍부한 상업적으로 유용한 지질을 생산하게 한다.
일부 양태에서, 특정 생물체로부터 유래된 소정의 PUFA 신타제 시스템은 특정 PUFA(들)을 생산할 것이고, 이에 의해 특정 생물체로부터 PUFA 신타제 시스템의 선별은 특정한 표적 또는 주요 PUFA를 생산할 것이다. 일부 양태에서, PUFA의 비율은 특정 PUFA 신타제 시스템의 선택, 및 당해 시스템이 발현되는 특정 조건에 대응하는 방법에 따라 달라질 수 있다. 예를 들면, 트라우스토키트리움 23B(ATCC No. 20892)로부터 PUFA 신타제의 사용은 또한 표적 또는 주요 PUFA로서 DHA 및 DPAn-6을 생산할 것이지만, 트라우스토키트리움 23B의 경우에는 DHA 대 DPAn-6의 비율이 약 10:1(및 약 8:1 내지 약 40:1의 범위일 수 있음)이고, 스키조키트리움에서는 당해 비가 통상 약 2.5:1이다. 일부 양태에서, 소정의 PUFA 신타제는 상이한 PUFA 신타제로부터의 단백질 및 도메인을 상호 혼합함으로써 변형될 수 있거나, 표적 PUFA 생성물 및/또는 비율을 변화시키기 위해 소정 PUFA 신타제의 도메인 또는 단백질을 변형시킬 수 있다.
일부 양태에서, PUFA를 생산하는 효소 시스템의 "중간 생성물" 또는 "부생성물(side product)"은 임의의 생성물 및 특히, 당해 시스템의 표적 또는 주요 PUFA(들)의 생산 결과로서 효소 시스템에 의해 생산되지만 주요 또는 표적 PUFA(들)가 아닌 지방산 생성물을 지칭한다. 일부 양태에서, 중간 및 부생성물은 야생형 식물 또는 지시된 유전자 변형을 위한 수용체로서 사용된 모 식물에 의해 자연적으로 생산되는 비표적 지방산을 포함할 수 있지만, 이들은 야생형 식물 또는 지시된 유전자 변형을 위한 수용체로서 사용된 모 식물에 의해 생산된 수준과 비교하여 유전자 변형의 결과로서 보다 큰 수준으로 생산되기 때문에 이제 중간 또는 부생성물로서 분류된다. 일부 양태에서, 하나의 효소 시스템의 주요 또는 표적 PUFA는 주요 또는 표적 생성물이 상이한 PUFA인 상이한 효소 시스템의 중간생성물일 수 있다. 예를 들면, EPA를 생산하기 위해 표준 경로를 사용하는 경우, GLA, DGLA 및 SDA 등의 지방산은 상당량으로 중간 생성물로서 생산된다(예: 미국 특허출원 공개번호 2004/0172682). 유사하게는, 및 미국 특허출원 공개번호 2004/0172682에 설명된 바와 같이, DHA를 생산하기 위해 표준 경로를 사용하는 경우, 상술한 지방산 이외에, ETA 및 EPA(그 중에서도 상기 제1 예에서 표적 PUFA)가 상당량으로 생산될 수 있고, 표적 PUFA 자체보다 전체 지방산 생성물과 관련하여 현저히 큰 양으로 존재할 수 있다.
일부 양태에서, 하나 이상의 다중불포화 지방산을 현저히 높은 수율로 생산하기 위해, 식물을 유전자 조작하여 PUFA 신타제 시스템을 식물 내로 도입할 수 있다. 식물은 PUFA 신타제를 내생적으로 함유하는 것으로 공지되지 않고, 따라서 본 발명은 독특한 지방산 생산능을 갖는 식물을 생산할 기회를 나타낸다. 본 발명은 동일한 식물에서 하나 이상의 PUFA를 생산하기 위해 유전자 조작된 식물을 제공하고, 이는, 이로써 한정되지 않지만, 이들의 임의의 조합을 포함하여, EPA, DHA, DPA (n3 또는 n6), ARA, GLA, SDA 등을 포함한다. 본 발명은 다수의 "고가 오일(designer oil)" 중의 어느 하나를 다양한 비율 및 형태로 생성하는 능력을 제공한다. 일부 양태에서, 본원에 기재된 특정 해양 생물체로부터 PUFA 신타제 시스템의 사용은 PUFA 생산 범위를 확장할 수 있고, 대부분의 작물 식물의 성장에 사용된 온도 범위에서 이러한 PUFA를 성공적으로 생산할 수 있다.
일부 양태에서, PUFA를 합성하는 시스템의 중간 또는 부생성물을 "실질적으로 포함하지 않는" 또는 실질량으로 존재하는 중간 또는 부생성물을 "갖지 않는"은, PUFA를 생산하는 효소 시스템의 도입 또는 존재의 결과로서 유전자 변형된 식물(및/또는 식물 부분 및/또는 종자 오일 분획)에서 생산되는 임의의 중간 또는 부생성물 지방산(비표적 PUFA)(예를 들면, 야생형 식물 또는 지시된 유전자 변형에 대한 수용체로서 사용된 모 식물에 의해 생산되지 않음)은 당해 식물에 의해 생산된 총 지방산의 약 10중량% 미만, 보다 바람직하게는 약 9중량% 미만, 보다 바람직하게는 8중량% 미만, 보다 바람직하게는 약 7중량% 미만, 보다 바람직하게는 약 6중량% 미만, 보다 바람직하게는 약 5중량% 미만, 보다 바람직하게는 약 4중량% 미만, 보다 바람직하게는 약 3중량% 미만, 보다 바라직하게는 약 2중량% 미만, 및 보다 바람직하게는 식물에 의해 생산된 총 지방산의 약 1중량% 미만, 보다 바람직하게는 식물에 의해 생산된 총 지방산의 약 0.5중량% 미만인 양으로 존재할 수 있다.
일부 양태에서, 본 발명의 유전자 변형된 식물 또는 본 발명의 유전자 변형된 식물로부터 수득된 오일 또는 종자는 검출가능한 양의 DHA(도코사헥사엔산(C22:6, n-3)) 또는 EPA(에이코사펜타엔산(C20:5, n-3))을 포함한다. 일부 양태에서, 본 발명의 유전자 변형된 식물 또는 본 발명의 유전자 변형된 식물로부터 수득된 오일 또는 종자는 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5% 또는 15% DHA를 포함한다. 유용한 범위는 임의의 이들 값 사이, 예를 들면, 0.01-15%, 0.05-10% 및 1-5% DHA에서 선택될 수 있다.
일부 양태에서, 본 발명의 유전자 변형된 식물 또는 본 발명의 유전자 변형된 식물로부터 수득된 오일 또는 종자는 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5% 또는 10% EPA를 포함한다. 유용한 범위는 임의의 이들 값 사이, 예를 들면, 0.01-10%, 0.05-5% 및 0.1-5% EPA에서 선택될 수 있다.
일부 양태에서, PUFA 신타제 시스템의 표적 생성물이 장쇄 PUFA, 예를 들면, DHA, DPA(n-6 또는 n-3) 또는 EPA인 경우, 이러한 PUFA 신타제 시스템으로 유전자 변형된 식물의 총 지질에 실질량으로 존재하지 않는 중간 생성물 및 부생성물은, 이로써 한정되는 것은 아니지만, 감마-리놀렌산(GLA; 18:3, n-6); 스테아리돈산(STA 또는 SDA; 18:4, n-3); 디호모-감마-리놀렌산(DGLA 또는 HGLA; 20:3, n-6), 아라키돈산(ARA, C20:4, n-6); 에이코산트리엔산(ETA; 20:3, n-9) 및 다양한 기타 중간 또는 부생성물, 예를 들면, 20:0; 20:1(△5); 20:1(△11); 20:2(△8,11); 20:2(△11,14); 20:3(△5,11,14); 20:3(△11,14,17); 메드산(20:3; △5,8,11); 또는 20:4(△5,1,14,17)을 포함할 수 있다.
본 발명에 따르는 식물의 유전자 변형은 식물에 의한 하나 이상의 PUFA를 생산할 수 있다. 일부 양태에서, 당해 식물에 의해 생산된 PUFA 프로파일 및 PUFA 비율은 PUFA 신타제가 유래된 생물체에 의해 생산된 PUFA 프로파일 또는 PUFA 비율과 반드시 동일한 것은 아니다.
일부 양태에서, 본 발명의 유전자 변형된 식물을 조작하여, PUFA 신타제의 활성에 의해 PUFA를 생산할 수 있다. 일부 양태에서, PUFA는 식물로부터 화합물을 추출하는 정제 공정을 통해 회수할 수 있다. 일부 양태에서, PUFA는 식물을 수거하여 회수할 수 있다. 일부 양태에서, PUFA는 식물로부터(예: 오일 종자로부터) 또는 식물 종자로부터 오일을 수거하여 회수할 수 있다. 일부 양태에서, 식물은 또한 자연 상태로 소비되거나, 소비가능한 제품으로 추가로 가공될 수 있다.
일부 양태에서, 본 발명의 유전자 변형된 식물은 하나 이상의 다중불포화 지방산을 생산할 수 있다. 일부 양태에서, 당해 식물은 적어도 하나의 PUFA(표적 PUFA)를 생산할 수 있고(예: 이의 성숙 종자에서, 오일 종자 식물인 경우, 또는 오일 종자 식물의 종자의 오일에서), 여기서 PUFA를 축적하는 식물 중의 전체 지방산 프로파일 또는 식물의 부분(예: 성숙 종자, 당해 식물이 오일 종자 식물인 경우, 또는 오일 종자 식물의 종자의 오일)은 검출가능한 양의 이러한 PUFA 또는 PUFA들을 포함한다. 일부 양태에서, 표적 PUFA는 적어도 20개 탄소 PUFA이고, 적어도 3개의 이중결합, 보다 바람직하게는 적어도 4개의 이중결합, 보다 바람직하게는 적어도 5개의 이중결합을 포함한다. 일부 양태에서, 표적 PUFA는 식물에 의해 자연적으로 생산되지 않는 PUFA일 수 있다. 일부 양태에서, PUFA를 축적하는 식물 또는 식물 부분(식물의 종자 오일 포함)에서 총 지방산 프로파일은 총 지방산중 적어도 0.1중량%의 표적 PUFA(들), 적어도 0.2중량%, 적어도 0.3중량%, 적어도 0.4중량%, 적어도 0.5중량%, 적어도 1중량%, 적어도 1.5중량%, 적어도 2중량%, 적어도 2.5중량%, 적어도 3중량%, 적어도 3.5중량%, 적어도 4중량%, 적어도 4.5중량%, 적어도 5중량%, 적어도 5.5중량%, 적어도 10중량%, 적어도 15중량%, 적어도 20중량%, 적어도 25중량%, 적어도 30중량%, 적어도 35중량%, 적어도 40중량%, 적어도 45중량%, 적어도 50중량%, 적어도 55중량%, 적어도 60중량%, 적어도 65중량%, 적어도 70중량%, 적어도 75중량%, 식물에 의해 생산된 총 지방산중 75중량% 이상의 적어도 하나의 다중불포화 지방산(표적 PUFA 또는 PUFA들), 또는 0.1중량% 내지 75중량% 사이의 임의의 퍼센트, 또는 75중량% 초과(100% 이하 또는 100%)의 표적 PUFA(들)(0.1% 증가량으로)을 포함한다.
본원에 일반적으로 사용된 바와 같이, PUFA 생산의 퍼센트 양은, 달리 명시되지 않는 한, 생물체(식물)에 의해 생산된 총 지방산의 중량 기준이다. 일부 양태에서, 식물에 의해 생산된 총 지방산은 지방산 메틸 에스테르(FAME) 제제의 기체 크로마토그래피(GC) 분석에 의해 측정한 중량%로서 나타내지만, 총 지방산의 측정은 이 방법으로 한정되지 않는다.
일부 양태에서, 본 발명의 식물(및/또는 식물 부분 또는 종자 오일 분획) 중의 총 지방산은 식물에 의해 생산된 총 지방산중 10중량% 미만, 9중량% 미만, 8중량% 미만, 7중량% 미만, 6중량% 미만, 5중량% 미만, 4중량% 미만, 3중량% 미만, 2중량% 미만, 1중량% 미만의, 하기 하나 이상으로부터 선택된 지방산: 감마-리놀렌산(GLA; 18:3, n-6); 스테아리돈산(STA 또는 SDA; 18:4, n-3); 디호모-감마-리놀렌산(DGLA 또는 HGLA; 20:3, n-6), 아라키돈산(ARA, C20:4, n-6); 에이코산트리엔산(ETA; 20:3, n-9) 및 다양한 기타 지방산, 예를 들면, 20:0; 20:1(△5); 20:1(△11); 20:2(△8,11); 20:2(△11,14); 20:3(△5,11,14); 20:3(△11,14,17); 메드산(20:3; △5,8,11); 또는 20:4(△5,1,14,17).
본 발명은 본원에 기재된 식물에 의해 생산된 임의의 종자 뿐만 아니라 본 발명의 식물 또는 종자에 의해 생산된 임의의 오일을 포함한다. 본 발명은 또한 본원에 기재된 식물, 종자 또는 오일을 사용하여 생산된 임의의 생성물을 포함한다.
본 발명의 유전자 변형된 생물체와 관련된 용도 및 생성물
본 발명은 상기 상세히 기재된 본 발명의 유전자 변형된 생물체(예: 식물)을 성장 또는 배양함으로써 PUFA를 생산하는 방법을 포함한다. 일부 양태에서, 이러한 방법은, 예를 들면, 적합한 환경(예: 토양)에서, 본원에서 상기 기재된 바와 같은 유전자 변형을 갖는 식물을 본 발명에 따라 성장시키는 단계를 포함한다.
본 발명은, 본 발명의 유전자 변형된 식물 또는 본 발명의 유전자 변형된 식물의 종자로부터 오일을 회수하는 단계를 포함하는, 적어도 하나의 PUFA를 포함하는 오일을 생산하는 방법을 포함한다. 본 발명은, 본 발명의 유전자 변형된 식물을 성장시키는 단계를 포함하는, 적어도 하나의 PUFA를 포함하는 오일을 생산하는 방법을 포함한다. 본 발명은, 본 발명의 유전자 변형된 식물의 종자로부터 오일을 회수하는 단계를 포함하는, 종자 오일에서 적어도 하나의 PUFA를 생산하는 방법을 포함한다. 본 발명은, 본 발명의 유전자 변형된 식물을 성장시키는 단계를 포함하는, 종자 오일에서 적어도 하나의 PUFA를 생산하는 방법을 포함한다.
본 발명은, 본 발명의 유전자 변형된 식물, 본 발명의 오일, 본 발명의 종자, 본 발명의 식료품, 본 발명의 기능성 식품 또는 본 발명의 약품을 개체에게 제공하는 단계를 포함하는, 적어도 하나의 PUFA를 함유하는 보충 또는 치료 생성물을 개체에게 제공하는 방법을 포함한다. 본 발명은 또한, 식물 또는 식물 세포를 (i) 적어도 하나의 다중불포화 지방산(PUFA)를 생산하는 조류 PUFA 신타제 시스템을 인코딩하는 핵산 서열 및 (ii) 포스포판테테이닐 보조인자를 조류 PUFA 신타제 시스템 ACP 도메인으로 전달하는 포스포판테테이닐 트랜스퍼라제(PPTase)를 인코딩하는 핵산 서열로 형질전환시키는 단계를 포함하는, 본 발명의 유전자 변형된 식물을 생산하는 방법을 포함한다. 일부 양태에서, 당해 방법은 식물 또는 식물 세포를 (iii) 장쇄 PUFA 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신세타제(ACoAS)를 인코딩하는 핵산 서열로 형질전환시킴을 추가로 포함한다.
일부 양태에서, 이러한 방법의 PUFA는 DHA 또는 EPA이다.
본 발명은 추가로, 본원에 기재된 생물체에 의해 생산된 임의의 오일 뿐만 아니라 본원에 기재된 임의의 생물체 또는 이의 부분(예: 식물, 식물 부분(예: 오일 종자) 또는 이의 제제 또는 분획)을 포함한다. 본 발명은 또한 본원에 기재된 생물체, 이의 부분 또는 오일을 사용하여 생산된 임의의 생성물을 포함한다.
본 발명은 본원에 기재된 본 발명에 따르는 유전자 변형된 생물체에 의해 생산된 생물체, 이의 부분 또는 오일(예: 본원에 기재된 바와 같이 유전자 변형된 식물)을 생성물에 첨가하는 단계를 포함하는, 적어도 하나의 지방산을 함유하는 생성물을 변형시키는 방법에 관한 것이다. 이러한 방법에 의해 생산되거나 본원에 기재된 생물체로부터 임의의 생물체, 이의 부분 또는 오일을 일반적으로 함유하는 임의의 생성물이 또한 본 발명에 의해 포함된다.
일부 양태에서, 당해 생성물은 식품, 식이 보충물, 약제 제형, 사람화 동물 밀크, 이유식, 영양 또는 기능성 식품으로 이루어진 그룹으로부터 선택된다. 적합한 약제학적 제형은, 이로써 한정되는 것은 아니지만, 소염 제형, 화학치료제, 활성 부형제, 골다공증 약물, 항우울제, 항경련제, 항-헬리코박터 필로리 약물, 신경퇴행성 질환 치료용 약물, 퇴화성 간 질환 치료용 약물, 항생제 및 콜레스테롤 저하 제형을 포함한다. 일부 양태에서, 당해 생성물은 만성 염증, 급성 염증, 위장 질환, 암, 악액질, 심장 재협착, 신경퇴행성 질환, 퇴행성 간 질환, 혈액 지질 질환, 골다공증, 골관절염, 자가면역 질환, 전자간증, 조산, 연령 관련 황반병, 폐 질환 및 퍼옥시좀 장애로 이루어진 그룹으로부터 선택된 증상을 치료하는데 사용된다.
일부 양태에서, 당해 생성물은 식료품 또는 기능성 식료품이다. 적합한 식료품은, 이로써 한정되는 것은 아니지만, 미세 제과품, 브레드 및 롤, 조식 시리얼, 가공 및 비가공 치즈, 조미료(케?, 마요네즈 등), 유제품(밀크, 요거트), 푸딩 및 젤라틴 디저트, 탄산 음료, 차, 분말형 음료 혼합물, 가공 어류 제품, 과일계 드링크, 츄잉 검, 경질 과자, 동결 유제품, 가공육 제품, 너트 및 너트계 스프레드, 파스타, 가공 가금 제품, 육즙 및 소스, 포테이토 칩 및 기타 칩 또는 크리스프(crips), 초콜렛 및 기타 과자류, 수프 및 수프 혼합물, 대두계 생성물(예: 밀크, 음료, 크림, 분말 크림), 식물유계 스프레드 및 식물계 음료를 포함한다.
본 발명의 일부 양태에서, 당해 생성물은 동물을 위한 사료 또는 밀(meal) 조성물 또는 사료 또는 밀 조성물용 첨가제이다. 용어 동물은 사람을 포함하는 모든 동물을 포함한다. 동물의 비제한적 예는 비반추동물(예: 돼지, 가금 또는 어류) 및 반추동물(예: 소, 양 및 말)이다. 용어 사료 및 사료 조성물은 동물에 의한 섭취에 적합하거나 이를 위해 의도된 임의의 화합물, 제제, 혼합물 또는 조성물을 의미한다.
일부 양태에서, 유전자 변형된 식물, 종자 또는 오일(예: 캐놀라)는 통상의 오일과 비교하여 감소된 수준의 다중불포화 지방산 및 증가된 수준의 일불포화된 올레산을 포함한다. 이러한 식물, 종자 또는 오일은, 예를 들면, 보다 높은 산화 안정성을 나타낼 수 있다. 일부 양태에서, 유전자 변형된 식물, 종자 또는 오일은 고도의 올레산 오일 배경(예: 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% 또는 99% 올레산)을 포함한다. 이러한 식물, 종자 또는 오일은, 예를 들면, 저장, 프라잉 및/또는 정련 동안 산화에 대해 덜 감수성일 수 있고/있거나 발연 없이 보다 고온으로 가열될 수 있고, 이에 의해 쿠킹 오일로서 보다 적합하게 한다. 일부 양태에서, 유전자 변형된 식물, 종자 또는 오일은 본원에 기재된 소정량의 DHA 및 고도의 올레산 오일 배경(예: 70% 이상의 양, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% 및 99% 올레산 및 이의 임의의 범위 포함)을 포함한다. 일부 양태에서, 유전자 변형된 식물, 종자 또는 오일은 본원에 기재된 소정량의 DHA 및 낮은 리놀렌산 배경(예: 10% 이하의 양, 9.5%, 9%, 8.5%, 8%, 7.5%, 7%, 6.5%, 6%, 5.5%, 5%, 4.5%, 4%, 3.5%, 3%, 2.5%, 2%, 1.5%, 1%, 0.05%, 0.02% 또는 0.01% 리놀렌산 및 이의 임의의 범위 포함)을 포함한다. 일부 양태에서, 유전자 변형된 식물, 종자 또는 오일은 본원에 기재된 소정량의 DHA 및 높은 올레산 오일 배경(예: 70% 이상의 양으로 존재, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% 및 99% 올레산 및 이의 임의의 범위 포함), 및 낮은 리놀렌산 배경(예: 10% 이하의 양, 9.5%, 9%, 8.5%, 8%, 7.5%, 7%, 6.5%, 6%, 5.5%, 5%, 4.5%, 4%, 3.5%, 3%, 2.5%, 2%, 1.5%, 1%, 0.05%, 0.02% 또는 0.01% 리놀렌산 및 이의 임의의 범위 포함)을 포함한다. 일부 양태에서, 이러한 유전자 변형된 식물, 종자 또는 오일(예: 캐놀라)는 본원에 기재된 생성물 내로 도입될 수 있다.
본 발명의 추가적인 목적, 잇점 및 신규한 특징은, 제한하는 것으로 의도되지 않는 하기 실시예를 참조함으로써 당해 기술분야의 통상의 지식을 가진 자에게 명백해질 것이다.
실시예
실시예 1
PUFA 신타제 OrfA , PUFA 신타제 OrfB , PUFA 신타제 OrfC , 아실- CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 의 코돈 최적화
스키조키트리움 종 ATCC_20888(GenBank ID: AF378327, GI:158518688)로부터의 PUFA OrfA, 스키조키트리움 종 ATCC_20888(GenBank ID: AF378328, GI:158518690)로부터의 PUFA OrfB, 스키조키트리움 종 ATCC_20888 및 트라우스토키트리움(미국 특허출원 공개번호 2008/0022422)으로부터의 키메라 PUFA OrfC("키메라 OrfC" 또는 "하이브리드 OrfC"), 스키조키트리움 종 ATCC_20888(미국 특허출원 공개번호 2007/0245431)로부터의 아실-CoA 신세타제, 노스톡 종 PCC 7120(GenBank ID: P37695, GI:20141367) 코딩 영역으로부터의 4' 포스포판테테이닐 트랜스퍼라제 HetI을 인코딩하는 DNA 서열의 분석은, 최적 식물 발현에 유해할 수 있는 비-최적 코돈 조성을 함유하는 몇몇 서열 모티브의 존재를 나타냈다. PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 단백질을 인코딩하는 유전자의 설계는, 자연에서 보다 "식물-유사"하거나 당해 서열 변형이 해독을 방해하지 않거나 비-최적 코돈 조성을 통해 mRNA 안정성을 생성하지 않는 DNA 서열을 형성하기 위해 최적화되었다.
유전자 코드의 중복/축퇴에 의해 제공된 가소성(예: 일부 아미노산은 하나 이상의 코돈에 의해 명시됨)에 기인하여, 상이한 생물체 또는 생물체 부류에서 게놈의 진화는 동의(synonymous) 코돈의 상이한 사용을 발생시켰다. 이러한 "코돈 바이어스"는 단백질 코돈 영역의 평균 염기 조성에 반영된다. 예를 들면, G+C 함량이 비교적 낮은 게놈을 갖는 생물체는 동의 코돈의 제3 위치에서 A 또는 T를 갖는 보다 많은 코돈을 이용하고, G+C 함량이 보다 높은 생물체는 제3 위치에서 G 또는 C를 갖는 보다 많은 코돈을 이용한다. 추가로, mRNA 내에 "소수(minor)" 코돈의 존재는, 특히 소수 코돈에 상응하는 하전된 tRNA의 상대적 풍부도가 낮은 경우, 당해 mRNA의 절대 해독 속도를 감소시킬 수 있다. 이러한 이유의 확장은, 개개 소수 코돈에 의한 해독 속도의 감소가 복수의 소수 코돈에 적어도 부가적일 수 있다는 것이다. 따라서, 소수 코돈의 상대 함량이 높은 mRNA는 상응하게 낮은 해독 속도를 가질 것이다. 이러한 속도는 인코딩된 단백질의 상응하게 낮은 수준에 의해 반영될 것이다.
캐놀라(또는 기타 식물, 예를 들면, 벼, 담배, 옥수수, 목면 또는 대두)에서 발현시키기 위해 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 단백질을 인코딩하는 유전자 조작에 있어서, 캐놀라용의 코돈 이용은 공중 이용가능한 데이터베이스(표 2)에 의해 입수되었다.
Figure pct00002
아미노산에 대한 잔여 코돈 선택의 배열을 계량하기 위해, 각 코돈에 대한 중량 평균 표시는 하기 식을 사용하여 계산했다(표 2):
C1의 중량 평균(%) = 1/(%C1 + %C2 + %C3 + 등) ×%C1 ×100
여기서, C1은 당해 코돈이고, %C2, %C3 등은 표 2의 잔여 동의 코돈의 캐놀라에 대한 값(%)의 평균을 나타낸다(관련 코돈에 대한 평균 % 값은 컬럼 C 및 G로부터 취함).
각 코돈에 대한 중량 평균(%)은 표 2의 컬럼 D 및 H에 제공되어 있다.
식물 발현을 위한 코딩 영역을 설계함에 있어서, 당해 식물에 의해 선호된 주요 ("제1 선택") 코돈 뿐만 아니라, 다중 선택이 존재하는 경우, 선호 코돈의 제2, 제3, 제4 등의 선택을 결정했다. 이어서, PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI의 동일한 아미노산 서열을 실질적으로 인코딩하지만 식물(제1 선호, 제2 선호, 제3 선호 또는 제4 선호 등) 코돈의 치환에 의해 아미노산 서열 내의 각 위치에서 아미노산을 특정함으로써 원형(original) DNA 서열(PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI을 인코딩함)과는 상이한 새로운 DNA 서열을 설계했다.
이어서, 새로운 서열을 당해 서열에서의 변형에 의해 생성된 제한 효소 부위에 대해 분석했다. 이어서, 동정된 부위는 당해 코돈을 제1, 제2, 제3 또는 제4 선택 선호 코돈으로 대체함으로써 변형시켰다. 이어서, 당해 서열을 추가로 분석하고, 변형시켜 TA 또는 GC 이중선의 빈도를 감소시켰다.
이들 서열의 분석은, 새로운 DNA 서열이 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 단백질의 아미노산 서열을 실질적으로 인코딩했지만, 캐놀라 유전자에서 발견된 빈번히 사용된 코돈의 계량된 코돈 분포를 사용하여 캐놀라에서 최적 발현에 대해 각각 설계되었음을 나타냈다. 특히, 새로운 DNA는 식물(제1 선호, 제2 서호, 제3 선호 또는 제4 선호) 코돈의 치환에 의해 단백질 아미노산 서열 내의 각 위치에서 적절한 아미노산을 특정함으로써 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 시타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI을 인코딩하는 원형 DNA 서열과는 상이했다.
식물 최적화 DNA 서열의 설계는, 표 2로부터 구성된 캐놀라 코돈 바이러스 표, 컬럼 D 및 H를 사용하여 서열번호 1, 서열번호 2, 서열번호 3, 서열번호 4 및 서열번호 5의 단백질 서열의 역-해독에 의해 개시했다. 아실-CoA 신세타제(서열번호 4)의 단백질 서열은 원형 서열로부터 변화되었고, 여기서 제2 아미노산 알라닌은 단백질로부터 제거되었다. 이어서, 개시 서열은, 코돈 변화(전체 중량 평균 코돈 표시를 유지하면서)를 보상하여, 제한 효소 인지 부위를 제거하거나 부가하고 고도의 안정성 쇄내 제2 구조체를 제거하고 식물에서 조작된 유전자의 클로닝 조작 또는 발현에 유해할 수 있는 다른 서열을 제거함으로써 변형되었다. 이어서, DNA 서열은, 변형에 의해 생성될 수 있는 제한 효소 인지 부위에 대해 분석했다. 동정된 부위는 관련 코돈을 제1, 제2, 제3 또는 제4 선택 선호 코돈으로 대체하여 추가로 변형시켰다. 목적하는 유전자의 전사 또는 해독에 영향을 미칠 수 있는 당해 서열 내의 다른 부위는 엑손:인트론 접합부(5' 또는 3'), 폴리 A 부가 시그널, 또는 RNA 폴리머라제 종결 시그널을 포함한다. 변형된 서열은 추가로 분석하고 추가로 변형시켜, TA 또는 CG 이중선의 빈도를 감소시키고 TG 또는 CT 이중선의 빈도를 증가시켰다. 이들 이중선 이외에, [G+C] 또는 [A+T]의 약 6개 이상의 보존적 잔기를 갖는 서열 블록은 당해 서열의 전사 또는 해독에 영향을 미칠 수 있다. 따라서, 이들 서열 블록은 또한 제1 또는 제2 선택 코돈을 다른 선호 선택 코돈으로 대체하여 변형시켰다. 드물게 사용된 코돈은 유전자 설계에 상당한 정도로 포함되지 않고, 코돈 조성 자체와는 상이한 설계 표준을 적용하는데 필요한 경우에만 사용된다(예: 제한 효소 인지 부위의 부가 또는 결실).
PUFA 신타제 OrfA에 의해 인코딩된 단백질은 17 내지 19개 아미노산 크기 범위의 10개 반복 "프롤린-알라닌" 도메인을 포함한다. 프롤린-알라닌 반복체 사이에는 87개 아미노산을 포함하는 9개의 보다 긴 반복 서열 도메인이 산재되어 있다. 이들 반복체의 아미노산 서열은 4개 위치에서만 상이하고, 상이한 위치 각각에서 2개의 아미노산 선택만이 존재했다. 클러스털 W 컴퓨터 프로그램을 사용한 9개 반복체의 아미노산 서열의 분석은 100%의 상동성 값 및 95.4%의 동일성 값을 생성했다. DNA 수준에서, 9개 반복체를 인코딩하는 서열은 100% 상동성 및 89.7% 동일성이 있고, 이는 각 반복체를 인코딩하는 261개 염기에서 27개 위치에서만 상이하다(27개 변화 중의 23개는 "침묵" 차이이고, 여기서 동일한 아미노산에 대한 동의 코돈은 교환됨).
표준 유전자 설계 공정은 이러한 크기의 복수 반복체에 대한 새로운 코돈 바이어스된 DNA 서열의 개발을 용이하게 수용할 수 없는데, 이는 고도 관련된 DNA 서열의 생성을 방지하기 위해, 다른 8개 반복체 중의 동일한 위치에서 이루어진 코돈 선택으로 개개 반복체에서 모든 코돈 선택을 연속적으로 대조해야 하기 때문이다. 87개 잔기 반복체 각각의 경우, 동일한 아미노산 서열을 인코딩하기 위한 4.5 × 1043개 이상의 가능한 DNA 서열이 존재한다(당해 서열에서 각 아미노산에 대한 동의 코돈 수의 곱으로 계산됨). 따라서, 동일하게 인코딩하는 DNA 서열을 생성하는데 이용가능한 매우 큰 컴퓨팅 공간이 존재한다. 하기 프로토콜은, 각각의 개개 반복체에 대한 (인 실리코) 다중 서열 디자인을 생성한 다음, 모든 서열 버젼을 대량으로 비교하여 당해 반복체를 인코딩하는 고도 분화된 서열을 나타내는 세트를 동정하는데 사용된 방법을 기재한다.
단계 1: 분리된 서열로서 각 반복된 아미노산 도메인을 인코딩하는 천연 DNA 서열을 추출한다.
단계 2: 개개 반복된 DNA 서열을 분리 서열로서 유전자 설계 프로그램(예: OPTGENETM, Ocimum Biosolutions, Hyderabad, India)에 이입한다. 단계 3 내지 5는 각각의 서열에 대해 별도로 수행된다.
단계 3: 표준 유전자 코드를 사용하여 DNA 서열을 해독한다.
단계 4: 표준 유전자 코드 및 적절한 코돈 바이어스 테이블을 사용하여 해독된 단백질 서열을 역해독시킨다. 이러한 예에서, 530 브라시카 나푸스 단백질 코딩 영역으로부터 수집한 바이어스된 코돈 테이블을 사용했고, 각각 생성된 서열은 "nap"("나푸스"의 경우) + 버젼 번호로 코드 명명되었다. 따라서, 반복체 1에 대한 제1 역-해독된, 코돈 바이어스된 서열은 "rpt1 nap1"로서 명명되었다. 이러한 실례에서, 당해 공정은 10회 수행하여, 반복체 1의 단백질 서열을 인코딩하는 10개 DNA 서열 버젼을 생성했다.
단계 5: 10개 서열 버젼을 상응하는 수의 텍스트 파일로 이출한다.
단계 6: 다른 반복된 서열 도메인의 각각에 대해 단계 3 내지 5를 반복한다. 이 실례에서, 총 90개 "nap" 서열 버젼이 생성되었다(각 반복된 요소에 대해 10개).
단계 7: 90개 서열 파일을 클러스털 W 프로그램 Mega 3.1(메가소프트웨어에서 접속됨)에 이입하고, 투입으로서 모든 90개 서열을 사용하여 다중 서열 정렬을 수행한다. 이들 서열은 단백질 코딩 영역의 세그먼트이기 때문에, 정렬은 허용된 갭 없이 수행된다. 클러스털 W 정렬 후, 근린 결합 트리(Neighbor-Joining tree)를 조립하고 가시화하고, 당해 단백질에서 9개 반복된 도메인 각각에 대해 10개 코돈 최적화 서열 중의 하나를 육안으로 골라낸다. 각각의 선택된 서열 버젼은 가장 깊이 분지된 트리 부분으로부터 선택된다.
단계 8: 각각의 반복된 도메인에 대한 선택된 서열을, 각각의 특정 반복체에 적절한 위치에서, 전체 단백질을 인코딩하는 코돈 최적화 DNA 서열 내로 도입한다.
단계9: 별도로 설계된 분화된 반복 요소를 포함하여 전체 코돈 최적화 서열의 최종 분석을 수행하여, 목적하지 않는 모티브, 제한 효소 인지 부위 등의 부재를 보장한다.
새롭게 설계된, 캐놀라 최적화 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 OrfC, 아실-CoA 신타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI DNA 서열은 각각 서열번호 6, 서열번호 7, 서열번호 8, 서열번호 9 및 서열번호 10에 수록되어 있다. 이들 코돈 최적화 서열은 명세서 전체에 걸쳐 버젼 3(v3)으로 간주한다. 버젼 2(v2)로서 표지된 서열은 원형 비코돈 최적화 서열을 기재한다.
생성 DNA 서열은 고도의 코돈 다양성을 갖고, 목적하는 염기 조성은 전략적으로 위치된 제한 효소 인지 부위를 함유하며, 유전자의 전자 또는 생성물 mRNA의 해독을 간섭할 수 있는 서열을 결여한다. 표 3, 표 4, 표 5, 표 6 및 표 7은 원형 유전자에서 발견된 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 단백질에 대한 코딩 서열의 코돈 조성, 식물 최적화 버젼 및 표 2, 컬럼 D 및 H로부터 계산된 식물 최적화 서열에 대한 코돈 조성 권고치를 나타낸다.
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코딩 영역 서열의 코돈 최적화가 완료된 후, 추가의 뉴클레오티드 서열을 최적화 코딩 영역 서열에 부가했다. 클로닝을 촉진시키기 위한 제한 부위, 코작(Kozak) 서열 및 추가 정지 코돈을 식물 최적화 코딩 서열에 부가했다. 또한, 제2 시리즈의 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 포스포판테테이닐 트랜스퍼라제 HetI 코딩 서열을 설계했고, 이는 아라비돕시스 탈리아나(Arabidopsis thaliana) 리불로스 비스포스페이트 카복실라제 소형 쇄 1A(GenBank ID: NM_202369.2)로부터의 엽록체 표적화 서열을 함유했다. 당해 서열 서열번호 11을 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC 및 포스포판테테이닐 트랜스퍼라제 HetI에 대해 상기한 코딩 서열에 부가했다. 서열번호 6, 서열번호 7, 서열번호 8 및 서열번호 10으로부터의 최소 메티오닌을 제거하고, 엽록체 표적화 서열로 대체했다. 엽록체 표적화 서열을 함유하는 서열은 명세서 전체에 걸쳐 버젼 4(v4)로 간주한다.
제2 엽록체 통과 펩티드를 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 포스포판테테이닐 트랜스퍼라제 HetI 코딩 서열에 부가했다. 이들 코딩 서열은 아실-ACP-티오에스테라제(GenBank ID: X73849.1)로부터의 엽록체 표적화 서열을 함유하도록 설계했다. 이 서열(서열번호 38)을 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC 및 포스포판테테이닐 트랜스퍼라제 HetI에 대해 상기한 코딩 서열에 부가했다. 서열번호 6, 서열번호 7, 서열번호 8, 서열번호 9 및 서열번호 10으로부터 최초 메티오닌을 제거하고, 엽록체 표적화 서열로 대체했다. 엽록체 표적화 서열을 함유하는 서열은 명세서 전체에 걸쳐 버젼 5(v5)로 간주한다.
식물 최적화 DNA 서열이 종이 또는 인 실리코 상에서 설계되면, 설계된 서열과 서열이 정확하게 상응하도록 실제 DNA 분자를 실험실에서 합성할 수 있다. 이러한 합성 DNA 분자를 클로닝하고, 달리는 이들이 자연 또는 천연 공급원으로부터 유래되는 것과 같이 정확하게 조작할 수 있다. 상기한 부가 서열을 함유하는 서열번호 6, 서열번호 7, 서열번호 8, 서열번호 9 및 서열번호 10을 포함하는 DNA 단편의 합성은 상업적 공급업자에 의해 수행되었다(Geneart Ag, Regensburg, Germany). 이어서, 합성 DNA를 발현 벡터 내로 클로닝하고, 실시예 2, 3 및 4에 기재된 바와 같이 아그로박테리움 및 캐놀라에 형질전환시켰다.
PUFA 신타제 OrfA 코딩 서열의 코돈 최적화와 함께 이러한 방법을 사용하는 것은, 반복된 서열 불안정성을 방지하도록 충분히 분화된 반복 프롤린-알라닌 서열의 선별을 생성했다. 이들 서열은 근린 결합 트리의 가장 깊은 분지로부터 선택되었다(즉, 이러한 서열 세트에서 서로 가장 멀리 관련됨). 스미스-와세르만 구형 정렬을 모든 쌍 조합물에 대해 수행했고, 상동성 범위는 76-77%의 가능한 중앙값과 함께 74-81%였다.
Figure pct00008
9개의 반복된 도메인에 대해 선택된 9개의 새롭게 설계된 코딩 영역의 클러스털 W 정렬(Vector NTI, Invitrogen, Carlsbad, CA)은 도 1에 제시되어 있다. 전체적으로, 당해 서열은, 100% 상동성 및 89.7% 동일성을 갖는 원형 서열과 비교하여, 93.1% 상동성 및 61.7% 동일성을 갖는다. 보다 큰 서열 분화는 10 이상의 서열 반복을 사용하고 컴퓨터 프로그램 또는 수학적 알고리즘을 사용하여 이들 서열로부터 선별(육안으로 서열을 선택하는 대신에)함으로써 달성될 수 있다. 그럼에도 불구하고, 예시된 서열은 고도로 분화성이고, 뉴클레오티드를 함유하는 안정한 폴리-뉴클레오티드 단편을 생성했다.
실시예 2
pDAB7361 , pDAB7362 , pDAB7363 , 및 추가의 작제물을 위한 플라스미드 작제
pDAB7361 의 작제
pDAB7361 플라스미드(도 2; 서열번호 35)는 다중-부위 입구 재조합 L-R 반응(Invitrogen, Carlsbad, CA)을 사용하여 작제하였다. pDAB7361은 다음과 같이 3개의 PUFA 신타제 식물 전사 단위(PTU), 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 절단된 파세올루스 불가리스 피토헤마글루티닌-L(Phaseolus vulgaris phytohemagglutinin-L) 유전자 프로모터(PvDlec2 프로모터 v2; GenBank 수탁번호 X06336), 아라비돕시스 탈리아나(Arabidopsis thaliana) AT2S3 유전자 5' 비해독 영역(2S 5' UTR; GenBank 수탁번호 NM_118850), 스키조키트리움종 다중불포화 지방산 신타제 개방 판독 프레임 A(Sz PUFA OrfA v2) 및 아라비돕시스 탈리아나 2S 알부민 유전자 3' 비해독 영역 터미네이터 v1(At2S SSP 터미네이터 v1; GenBank 수탁번호 M22035)을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, 스키조키트리움 종 다중불포화 지방산 신타제 개방 판독 프레임 B(SzPUFA OrfB v3) 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, 스키조키트리움 종 및 트라우스토키트리움 종 다중불포화 지방산 신타제 개방 판독 프레임 C(hSzThPUFA OrfC v3) 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, 스키조키트리움 종 아실-CoA 신세타제 (SzACS-2 v3) 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, 노스톡 종(Nostoc sp .) 4' 포스포판테테이닐 트랜스퍼라제 HetI(NoHetI v3) 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7355, pDAB7335, pDAB7336, pDAB7339 및 pDAB7333을 재조합하여 pDAB7361을 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동[참조: Toro et al., PNAS 85( 22): 8558-8562; 1988] 및 T-스탠드 탑재 서열[T-DNA 탑재 A 및 T-DNA 탑재 B; Gardner et al., Science 231:725-727; 1986 및 WO 2001/025459 A1] 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: 카사바 정맥 모자이크 바이러스 프로모터[참조: CsVMV 프로모터 v2; Verdaguer et al., Plant Molecular Biology 31:1129-1139; 1996], 포스피노트리신 아세틸 트랜스퍼라제[참조: PAT v5; Wohlleben et al., 유전자 70:25-37; 1988] 및 아그로박테리움 투메파시엔스(Agrobacterium tumefaciens ) ORF1 3' 비해독 영역[참조: AtuORF1 3' UTR v4; Huang et al., J. Bacteriol . 172:1814-1822; 1990]을 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 5개의 PTU의 도입에 대해 시험하였다.
pDAB7362 의 작제
pDAB7362 플라스미드(도 3; 서열번호 36)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB7362는 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 서열 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzACS-2 v3 유전자 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7334, pDAB7335, pDAB7336, pDAB7339 및 pDAB7333을 재조합하여 pDAB7362를 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 5개의 PTU의 도입에 대해 시험하였다.
pDAB7363 의 작제
pDAB7363(도 4; 서열번호 37)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB7363은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU 및 하나의 포스포판테테이닐 트랜스퍼라제 PTU 서열을 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v4 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v4 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v4 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzACS-2 v3 유전자 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v4 및 At2S SSP 터미네이터 v1을 함유한다. 또한, 모든 PTU는 라벨 "v4"로 지시된 아라비돕시스 탈리아나 리불로스 비스포스페이트 카복실레이트 작은 쇄 1A 엽록체 표적화 서열을 함유한다.
플라스미드 pDAB7340, pDAB7341, pDAB7342, pDAB7344 및 pDAB7333을 재조합하여 pDAB7363을 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 5개의 PTU의 도입에 대해 시험하였다.
pDAB7365 의 작제
pDAB7365는 SzPUFA OrfA v2, SzPUFA OrfB v2, hSzThPUFA OrfC v2, SzACS-2 v2 및 NoHetI v2의 천연 비코돈 최적화 버젼을 함유하도록 작제한 이원 플라스미드이다. pDAB7365 플라스미드(도 19; 서열번호 39)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB7365는 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v2 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v2 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfC v2 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzACS-2 v2 유전자 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v2 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7355, pDAB7356, pDAB7357, pDAB7360 및 pDAB7333을 재조합하여 pDAB7365를 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v2, SzPUFA OrfB v2, SzPUFA OrfC v2, SzACS-2 v2, NoHetI v2. pDAB7333은, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3'TR v4를 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 6개의 PTU의 도입에 대해 시험하였다.
pDAB7368 의 작제
pDAB7368은 SzPUFA OrfA v2, SzPUFA OrfB v2, hSzThPUFA OrfC v2 및 NoHetI v2의 천연 비코돈 최적화 버젼을 함유하도록 작제한 이원 플라스미드이다. 이 작제물은 SzACS-2 코딩 서열을 함유하지 않는다. pDAB7368 플라스미드(도 20; 서열번호 40)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB7368은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v2 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v2 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfC v2 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v2 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7355, pDAB7356, pDAB7357, pDAB7359 및 pDAB7333을 재조합하여 pDAB7368을 형성하였다. 구체적으로, 상기한 4개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v2, SzPUFA OrfB v2, SzPUFA OrfC v2, NoHetI v2. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3'TR v4를 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 5개의 PTU의 도입에 대해 시험하였다.
pDAB7369 의 작제
pDAB7369는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3 및 NoHetI v3의 재조립된 코돈 최적화 버젼을 함유하도록 작제된 이원 플라스미드이다. 이 작제물은 SzACS-2 코딩 서열 PTU를 함유하지 않는다. pDAB7369 플라스미드(도 21; 서열번호 41)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB7369는 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7334, pDAB7335, pDAB7336, pDAB7338 및 pDAB7333을 재조합하여 pDAB7369를 형성하였다. 구체적으로, 상기한 4개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 5개의 PTU의 도입에 대해 시험하였다.
pDAB7370 의 작제
pDAB7370은 코딩 서열의 아미노 말단에 융합된 리블로스 비스포스페이트 카복실레이트 작은 쇄 1A(SSU-TP v1로서 표지됨)를 함유하는 SzPUFA OrfA v4, SzPUFA OrfB v4, hSzThPUFA OrfC v4 및 NoHetI v4의 재조립된 코돈 최적화 버젼을 함유하도록 작제된 이원 플라스미드이다. 이 작제물은 SzACS-2 코딩 서열 PTU을 함유하지 않는다. pDAB7370 플라스미드(도 22; 서열번호 42)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB7370은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v4 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v4 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v4 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v4 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7340, pDAB7341, pDAB7342, pDAB7343 및 pDAB7333을 재조합하여 pDAB7370을 형성하였다. 구체적으로, 상기한 4개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v4, SzPUFA OrfB v4, hSzThPUFA OrfC v4, NoHetI v4. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3'TR v4를 또한 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 5개의 PTU의 도입에 대해 시험하였다.
pDAB100518 의 작제
pDAB100518은 코딩 서열의 아미노 말단에 융합된 아실-ACP-티오에스테라제로부터의 엽록체 통과 펩티드(티오에스테라제 통과 펩티드로서 표지됨)를 함유하는 SzPUFA OrfA v5, SzPUFA OrfB v5, hSzThPUFA OrfC v5 및 NoHetI v5의 재조합된 코돈 최적화 버젼을 함유하도록 작제된 이원 플라스미드이다. 또한, 당해 플라스미드는 엽록체 통과 펩티드를 포함하지 않는 SzACS-2 v3 코딩 서열 PTU를 함유한다. pDAB100518 플라스미드(도 23; 서열번호 43)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB100518은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v5 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v5 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v5 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzACS-2 v3 유전자 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v5 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB100517, pDAB100514, pDAB100511, pDAB100515 및 pDAB7333을 재조합하여 pDAB100518을 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v5, SzPUFA OrfB v5, hSzThPUFA OrfC v5, SzACS-2 v3, NoHetI v5. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 또한 함유한다. 이어서, 6개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 6개의 PTU의 도입에 대해 시험하였다.
pDAB101476 의 작제
pDAB101476은 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3 및 NoHetI v3의 재조립된 코돈 최적화 버젼을 함유하도록 작제된 이원 플라스미드이다. SzACS-2 v2 유전자 서열은 천연 비코돈 최적화 버젼이다. pDAB101476 플라스미드(도 24; 서열번호 44)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB101476은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzACS-2 v2 유전자 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7334, pDAB7335, pDAB7336, pDAB101471 및 pDAB7333을 재조합하여 pDAB101476을 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, SzACS-2 v2, NoHetI v3. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3'UTR v4를 또한 함유한다. 이어서, 6개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 6개의 PTU의 도입에 대해 시험하였다.
pDAB101477 의 작제
pDAB101477은 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3 및 NoHetI v3의 재조립된 코돈 최적화 버젼을 함유하도록 작제된 이원 플라스미드이다. pDAB101477 플라스미드(도 25; 서열번호 45)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제하였다. pDAB101477은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzACS-2 v4 유전자 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7334, pDAB7335, pDAB7336, pDAB101472 및 pDAB7333을 재조합하여 pDAB101477을 형성하였다. 구체적으로, 상기한 5개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자의 순서는 다음과 같다: SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, SzACS-2 v4, NoHetI v3. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스탠드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3'UTR v4를 또한 함유한다. 이어서, 6개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석을 갖는 6개의 PTU의 도입에 대해 시험하였다.
실시예 3
플라스미드 pDAB7361 , pDAB7362 , pDAB7363 을 위한 아그로박테리움 균주 제조
pDAB7361, pDAB7362 및 pDAB7363 플라스미드를 표준 전기천공법을 사용하여 아그로박테리움 투메파시엔스로 형질전환시켰다. 구체적으로, 아그로박테리움 투메파시엔스 균주 Z707S[참조: Hepburn et al. J. Gen . Microbiol. 131:2961-2969 (1985)]는 pDAB7361, pDAB7362 또는 pDAB7363 플라스미드로 전기천공시켰다. 플라스미드를 함유한 아그로박테리움의 형질전환된 콜로니를 선택하고, 제한 효소 소화를 사용하여 확인하였다. pDAB7361, pDAB7362 또는 pDAB7363을 함유하는 아그로박테리움 균주를 -80℃에서 글리세롤 스톡으로서 저장하였다.
실시예 4
캐놀라의 아그로박테리움 매개된 형질전환
아그로박테리움 제조
pDAB7361, pDAB7362 또는 pDAB7363을 함유하는 아그로박테리움 균주의 글리세롤 스톡의 루프를 사용하여 스트렙토마이신(100mg/ml) 및 스펙티노마이신(50mg/ml)을 함유하는 YEP(박토 펩톤 20.0gm/L 및 효모 추출물 10.0gm/L) 플레이트를 스트리킹하고 28℃에서 2일 동안 인큐베이션시켰다. 이어서, 2일 스트리킹 플레이트의 루프를 멸균 500mL 배플(baffled) 플라스크(들) 속에서 스트렙토마이신(100mg/ml) 및 스펙티노마이신(50mg/ml)을 갖는 150mL 개질된 YEP 액체 속에서 접종시키고, 28℃에서 200rpm으로 진탕시켰다. 배양액을 M- 배지(LS 염, 3% 글루코스, 개질된 B5 비타민, 1μM 키네틴, 1μM 2,4-D, pH 5.8)에 재현탁시키고, 캐놀라 배축(hypocotyl)의 형질전환 전에 적합한 밀도(50 Klett 단위)로 희석시켰다.
캐놀라 형질전환
종자 발아: 캐놀라 종자(다양한 넥세라(Nexera) 710)를 10% 클로록스에서 10분 동안 표면 멸균시키고, 멸균 증류수로 3회 세정하였다(종자는 이 공정 동안 강 여과기에 함유시킨다). 종자를 피타트레이(phytatry)에 피타트레이당 25개 종자를 함유하는 1/2 MS 캐놀라 배지(1/2 MS, 2% 수크로즈, 0.8% 한천)에서 발아를 위해 심고, 25℃에서 광주기 16시간 명, 8시간 암으로 설정된 성장 섭생으로 퍼시벌 챔버에 위치시키고, 5일 동안 발아시켰다.
전처리: 5일째에, 약 3mm 배축 세그먼트를 무균 상태로 절개하여 뿌리 및 싹 부분을 버렸다(배축의 건조는 이들을 절개 공정 동안 10ml의 멸균 밀리Q 수에 위치시킴으로써 억제하였다). 배축 세그먼트를 22 내지 23℃에서 설정된 성장 섭생(광주기 16시간 명, 8시간 암)으로 퍼시벌 챔버에서 3일 전처리하기 위한 유합조직 유도 배지 MSK1D1(MS, 1mg/l 키네틴, 1mg/l 2,4-D, 3% 수크로즈, 0.7% Phytagar) 상의 멸균 여과지 상에 수평으로 위치시켰다.
아그로박테리움과 공동 배양: 아그로박테리윰 처리 전날, 적합한 항생제를 함유하는 YEP 배지의 플라스크를 인큐베이션시켰다. 배축 세그먼트를 여과지로부터 10ml 액체 M 배지를 함유하는 빈 100x25mm 페트리 접시로 이동시켜 배축 세그먼트가 건조되지 않도록 하였다. 이 단계에서 세그먼트를 퍼내어 이동시키기 위해 주걱을 사용하였다. 액체 M 배지를 피펫으로 제거하고, 40ml의 아그로박테리움 현탁액을 페트리 접시(40ml의 아그로박테리윰 용액을 갖는 500 세그먼트)에 첨가하였다. 당해 세그먼트를 30분 동안 페트리 접시의 주기적 와동으로 처리하여 체류된 배축이 아그로박테리움 용액에 침지되도록 하였다. 처리 기간 말기에, 아그로박테리움 용액을 폐기물 비이커 속에서 피펫팅하고, 오토클레이빙하여 버렸다(아그로박테리움 용액을 완전히 제거하여 아그로박테리움의 과성장을 억제하였다). 처리된 배축을 겸자를 사용하여 여과지와 함께 MSK1D1를 함유하는 원래 플레이트로 이동시켰다(세그먼트가 건조되지 않았음을 보장하기 위해 주의를 하였다). 대조군 세그먼트과 함께 배축 세그먼트를 감소된 빛의 세기하에 퍼시벌 챔버로 반환시키고(플레이트를 알루미늄 호일로 덮어서), 처리된 배축을 아그로박테리움으로 3일 동안 공동 배양하였다.
선택 배지 상의 유합조직 유도: 공동 배양 3일 후, 배축 세그먼트를 개별적으로 겸자를 사용하여 유합조직 유도 배지 MSK1D1H1(MS, 1mg/l 키네틴, 1mg/l 2,4-D, 0.5gm/l MES, 5mg/l AgNO3, 300mg/l 티멘틴, 200mg/l 카베니실린, 1mg/l Herbiace, 3% 수크로즈, 0.7% Phytagar) 상으로 이동시켰다. 배축 세그먼트를 배지 상에 고정시키지만, 배지에 매립시키지는 않았다.
선택 및 싹 재생: 유합조직 유도 배지 상에서 7일 후, 유합조직화 배축 세그먼트를 선택 MSB3Z1H1(MS, 3mg/l BAP, 1mg/l 제아틴, 0.5gm/l MES, 5mg/l AgNO3, 300mg/l 티멘틴, 200mg/l 카베니실린, 1mg/l Herbiace, 3% 수크로즈, 0.7% Phytagar)와 함께 싹 재생 배지 1로 이동시켰다. 14일 후, 싹과 함께 배축을 증기된 선택 MSB3Z1H3(MS, 3mg/l BAP, 1mg/l 제아틴, 0.5gm/l MES, 5mg/l AgNO3, 300mg/l 티멘틴, 200mg/l 카베니실린, 3mg/l HERBIACE, 3% 수크로스, 0.7% Phytagar)와 함께 재생 배지 2로 이동시켰다.
싹 신장: 14일 후, 싹을 갖는 세그먼트를 싹 신장 배지 MSMESH5(MS, 300mg/l 티멘틴, 5mg/l Herbiace, 2% 수크로스, 0.7% TC 한천)으로 이동시켰다. 이미 신장된 싹을 분리하고, MSMESH5로 이동시켰다. 14일 후, 제1 라운드에서 신장되지 않은 잔류하는 싹을 MSMESH5 상에 위치시키고, 동일한 조성의 새로운 선택 배지로 이동시켰다. 이 단계에서, 모든 잔류하는 배축 세그먼트를 버렸다.
2주 후, MSB3Z1H3 배지 상에서 신장된 싹을 분리하고 MSMESH5 배지로 이동시켰다. MSMESH5 상의 제1 라운드에서 신장되지 않은 잔류하는 싹을 분리하고, 동일 조성의 새로운 선택 배지로 이동시켰다. 이 단게에서, 모든 자륜하는 배축 세그먼트를 버렸다.
뿌리 유도: 14일 후, 싹을 뿌리 유도용 MSMEST 배지(MS, 0.5g/l MES, 300mg/l 티멘틴, 2% 수크로즈, 0.7% TC 한천)으로 이동시켰다. MSMEST 배지 상의 제1 이동에서 뿌리박히지 않은 싹을 뿌리박힌 식물이 수득될 때까지 MSMEST 배지 상의 제2 또는 제3 주기를 위해 이동시켰다. MSMEST 배지 상의 제1 이동에서 연장되거나 뿌리 박히지 않은 싹을 뿌리박힌 식물이 수득될 때까지 MSMEST 배지 상의 제2 또는 제3 주기를 위해 이동시켰다.
PCR 분석: 싹을 MSMESH5 배지 상에서 14일 이상 동안 배양한 후, PCR용 시료를 분리시켰다. 녹색 싹으로부터 잎 조직을 PAT 선택가능한 표지 유전자의 존재에 대해 PCR로 시험하였다. 모든 식물 황백화 싹을 버리고, PAT 분석에 적용하지 않았다. PCR 반응에 양성이었던 시료를 유지시키고, 싹을 MSMEST 배지 상에 정치시켜 뿌리를 신장시키고 발달시켰다. PCR 분석에 따라 음성이었던 싹을 버렸다.
MSMESH5 또는 MSMEST 상에서 뿌리박히고, PCR-양성이었던 식물을 토양에 이식용으로 제공하였다. 경화 후, T0 캐놀라 식물을 이식유전자 PTU 카세트 모두를 함유하는 이벤트에 대해 추가로 분석한 다음, 식물을 온실로 이동시키고, 성숙하게 성장시키고, 종자를 추가의 분석을 위해 수거하였다.
실시예 5
유전자변형 캐놀라 중의 코딩 영역의 카피 수 분석 및 검출
실시예 4로부터 선택된 T0 식물을 추가로 분석하여 각각의 변형유전자 PTU 발현 카세트가 함유된 식물을 동정하였다. 유입자 및 가수분해 프로브 분석을 수행하여 추정적으로 형질전환된 T0 식물의 시료를 초기에 스크리닝하여 PAT 발현 카세트가 함유된 이벤트를 동정하였다. 후속적인 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 유전자 발현 카세트의 PCR 분석을 완료하여 식물을 형질전환시키는데 사용된 이원 벡터로부터 각각 유전자 발현 카세트 PTU가 함유된 식물을 추가로 동정하였다. 모든 PTU를 함유하는 이벤트를 T1 식물로의 발전을 위해 선택하였다.
조직 시료를 96-웰 수집 플레이트에서 수집하고, 2일 동안 동결건조시켰다. 조직 침수연(Tissue maceration)은 클레코 조직 분쇄기 및 텅스텐 비드(Kleco, Visalia, CA)를 사용하여 수행하였다. 조직 침수연, 이어서 게놈 DNA를 제조업자의 제시된 프로토콜에 따라 DNeasy 96 플랜트 키트(Qiagen, Germantown, MD)를 사용하여 고처리량 포맷으로 분리시켰다.
gDNA는 Quant-IT Pico Green DNA 분석 키트(Molecular Probes, Invitrogen, Carlsbad, CA)에 의해 정량화하였다. 정량화된 gDNA를 Invader® 분석을 위해 10ng/㎕로, 가수분해 프로브 분석을 위해 2ng/㎕로 Biorobot3000 자동화 액체 취급기(Qiagen, Germantown, MD)를 사용하여 조정하였다.
통상적인 INVADER® 분석은 셋째 웨이브 기술(Madison, WI)에 의해 캐놀라 내에서 팻(pat) 분석용으로 개발되었다. gDNA 시료(7.5㎕의 10ng/㎕ gDNA)을 먼저 95℃에서 10분 동안 인큐베이션시켜 96-웰 플레이트 포맷에서 변성시킨 다음, 주위 온도로 냉각시켰다. 이어서, 7.5㎕의 마스터 믹스[팻 및 HMG 내부 참조 유전자용 3㎕의 프로브 믹스(Weng, 2005) Weng H. et al., (2005). J. AOAC Int . 88(2):577-84., 3.5㎕의 Cleavase XI FRET 믹스 및 1㎕의 Cleavase XI 효소/MgCl2 용액]를 각 웰에 첨가하고, 시료를 광유로 도포하였다. 플레이트를 밀봉시키고, 바이오라드 테트라드 써머사이클러(BioRad Tetrad thermocycler)에서 63℃에서 1시간 동안 배양하였다. 플레이트를 형광 플레이트 판독기 상에서 판독하기 전에 주위 온도로 냉각시켰다. 모든 플레이트는 1 카피, 2 카피 및 4 카피 표준 뿐만 아니라 야생형 대조군 시료 및 어떤 시료도 함유하지 않는 블랭크 웰을 함유한다.
판독치를 두 FAM(λ 485-528nm) 및 RED(λ 560-620nm) 채널에 대해 수집하고, 이들로부터 각 채널에 대해 0(즉, 배경) 이상의 중첩을 시료 원(raw) 시그널을 주형 없는 원 시그널로 나누어 각 시료에 대해 측정하였다. 이 데이터로부터, 표준 곡선을 작제하고, 최선의 적합도를 선형 회귀 분석으로 측정하였다. 이 적합도로부터 동정된 파라미터를 사용하여, 명백한 팻 카피 수를 각 시료에 대해 추산하였다.
TAQMAN® 분석과 유사한, 가수분해 프로브 분석에 의한 변형유전자 카피 수 측정을 LIGHTCYCLER® 480 시스템(Roche Applied Science, Indianapolis, IN)을 사용하여 실시간 PCR로 수행하였다. 분석은 LIGHTCYCLER® 프로브 디자인 소프트웨어 2.0을 사용하는 팻 및 내부 참조 유전자 HMG용으로 고안되었다. 증폭을 위해, LIGHTCYCLERR® 480 프로브스 마스터 믹스(Roche Applied Science, Indianapolis, IN)를 0.4μM의 각 프라이머 및 0.2μM의 각 프로브(표 8)를 함유하는 10μL 용적 복합 반응으로 1X 최종 농도에서 제조하였다. 2단계 증폭 반응을 형광 포착으로 60℃에서 35초 동안 확장하여 수행하였다. 모든 시료를 3회 수행하고, 평균 순환 임계(Ct) 값을 각 시료의 분석용으로 사용하였다.
실시간 PCR 데이터의 분석은 상대적 양 모듈을 사용하는 LIGHTCYCLER®소프트웨어 릴리스 1.5를 사용하여 수행하고, ΔΔCt 방법에 기초한다. 이를 위해, 단일 카피 구경측정기 및 공지된 2 카피 체크(check)로부터 gDNA의 시료를 각 작동에 포함시켰다(상기 유입자 분석용으로 사용된 것들과 동일함).
T0 식물 이벤트에 함유된 기타 유전자 발현 카세트의 존재는 개별적인 PCR 반응으로 검출되었다. 이러한 5개 PTU의 코딩 영역에 특이적인 프라이머 쌍(표 9)을 검출용으로 사용하였다.
Figure pct00009
PUFA 신타제 OrfA PCR 반응은 유전자 서열의 개방 판독 프레임을 증폭시키기 위한 2개의 개별적인 PCR 반응 및 상이한 조건(예: PCR 프라이머 및 순환 조건)을 필요로 한다. 모든 PCR 반응은 제조업자 지침당 EX-TAQ PCR 키트(TaKaRa Biotechnology Inc. Otsu, Shiga, Japan)를 사용하여 35회 순환으로 표 10에 기술된 조건을 사용하여 완료시켰다. PCR 생성물을 분해하고, TAE 아가로즈 겔 전기영동을 사용하여 동정하였다. 전체 길이 PTu의 존재를 지시하는 PCR 생성물에 대한 예상된 겔 단편 크기는 표 10에서 "예상된 크기" 컬럼에 기술된다.
Figure pct00010
총 197개 캐놀라 이벤트가 유입자 및 가수분해 프로브 실험으로부터 팻 양성으로서 동정되었다. 이러한 이벤트 중 15개는 식물을 형질전환시키는데 사용된 이원체 내에 함유된 모두 5개의 유전자 발현 카세트(PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI)에 대한 PCR 증폭자를 생성했다. 표 11은 도코사헥사엔산(DHA)의 생성으로부터 추가로 분석된 15개 이벤트를 제공한다. 이러한 T0 캐놀라 식물을 온실에서 성숙하게 성장시키고, 후속적으로 자체 수정시켰다. 성숙한 T1 종자를 수거하고, GC-FAME 분석을 통해 DHA에 대해 분석하였다.
Figure pct00011

실시예 6
유전자변형 캐놀라 종자 지질에서 DHA 의 검출
캐놀라 종자 시료(단일 종자 또는 벌크화 시료)을 트리아실글리세롤 내부 표준으로서 트리헵타데카노인((Nu-Chek prep)을 함유하는 헵탄 중에서 강철 볼 밀(steel ball mill)을 사용하여 균질화했다. 균질화 전에, 메탄올 중의 0.25M의 새로 제조된 나트륨 메톡사이드(Sigma-Aldrich, St. Louis, MO)의 용액을 첨가하였다. 추출은 40℃에서 일정하게 진탕시키면서 수행하였다. 회수는 메틸화 대체물 C17 지방산의 회수로 입증되었다. FAME(지방산 메틸 에스테르)의 추출을 3회 반복하고, 헵탄 층을 분석하기 전에 풀링하였다. 반응의 완료는 넷째 추출/유도체화에서 내생적 FAME의 존재를 체크하여 입증하였다. 생성되는 FAME를 SGE로부터 모세관 컬럼 BPX 70(15 m x 0.25 mm x 0.25μM)를 사용하는 GC-FID로 분석하였다. 각각의 FAME를 체류 시간에 의해 동정하고, 적합한 장쇄 다중불포화 지방산(Nu-Chek Prep, Elysian MN)을 첨가하면서 보정 표준으로서 Matreya LLC(Pleasant Gap, PA)로부터 평지씨 오일 참조 믹스의 주입으로 정량화하였다.
7개 이벤트로부터의 종자에 상응하는 FAME 추출물은 T1 종자의 GC-FAME 분석에 따르는 DHA 및 DPA (n-6)에 상응하는 피크를 함유하는 것으로 밝혀졌다(이하 표 12에서 표화됨). 표 12는 단일 종자에서 관찰된 DHA의 최대 함량과 같이, DHA-함유 종자의 수가 가변적이다(캐놀라 게놈에 삽입된 유전자변형 세트의 다양한 카피의 분리로부터 기대된 바와 같이)는 것을 나타낸다.
Figure pct00012
a. 검출가능한 DHA에 함유된 종자의 숫자/T1 벌크로부터 분석된 종자의 총 숫자
b. 모든 DHA-양성 종자의 평균 DHA 함량(총 지질%)
c. 모든 DHA-양성 종자의 평균 PUFA 함량(총 지질%)
d. DHAn-3/총 LC-PUFA(DHA+DPAn-6)의 평균 비율%
e. 단일 종자에서 관찰된 최고 DHA 함량
추가의 이벤트로부터의 개발 종자를 분석하고, DHA를 함유하는 것으로 밝혀졌지만, 성숙한 식물은 추가의 분석에 불충분한 T1 종자를 수득하였다. 장쇄 다중불포화 지방산(LC-PUFA) 피크 동일성은 질량 분광학 분석에 의해 확인하였고, 인증 표준(Nu-Chek Prep, Elysian MN)과 비교하였다.
하나의 이벤트(이벤트 5197[14]-032.002)로부터의 T1 종자의 DHA 함량에 대한 단일 종자 분석은 도 5에 도시한다. 단일 종자는 1% 이하의 DHA(총 FAME이 %로서)를 함유한다. DHA 수준은 DHA 생성 유전자를 함유하는 단일 서식지의 분리를 반영하는 3개의 부류(0, 약 0.4% 및 약 0.9% DHA)로 분리하는 것으로 나타난다.
이러한 데이터는 DHA가 플라스미드 pDAB7361, pDAB7362 및 pDAB7363으로 형질전환된 식물에서 생성되었음을 지시한다. pDAB7362 플라스미드는 파세올루스 불가리스 피토헤마글루티닌-L 유전자 프로모터로 구동되는 (PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI를 인코딩하는) 모두 5개 유전자의 식물 최적화 버젼을 함유한다. pDAB7361에서, PUFA 신타제 OrfA (SzOrfA v2)의 천연 유전자 서열은 식물 최적화 버젼(SzOrfA v3)을 대체한다. pDAB7363은 또한 아라비돕시스 탈리아나 리불로스 비스포스페이트 카복실라제 작은 쇄 1A 엽록체 통과 펩티드를 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC 및 4' 포스포판테테이닐 트랜스퍼라제 HetI의 N-말단에 첨가하여 이들 폴리펩티드를 색소체에서 표적화한다는 것 이외에는 pDAB7362와 유사하다.
실시예 7
캐놀라 종자에서 PUFA 신타제 단백질의 검출
PUFA 신타제 폴리펩티드는 웨스턴 블롯에 의해 성숙한 유전자변형 종자 시료에서 검출시켰다. 종자는 클레코 비드 비터(Kleco Bead Beater)(Garcia Machine, Visalia, CA) 중의 2개의 스테인레스 강철 비드로 무수 종자를 열분해시켜 분석용으로 제조하였다. 추출 완충제를 첨가하고(50mM 트리스, 10 mM EDTA, 2% SDS), 시료 튜브를 30분 동안 완만하게 진동시켰다. 시료를 3000rcf에서 30분 동안 원심분리했다. 상청액을 수집하여 분석용으로 사용하였다. 종자 추출물 중의 전체 가용성 단백질의 양은 로리 분석(Lowry assay)(BioRad, Hercules, CA)으로 측정하였다. 시료는 1.55mg/ml 총 가용성 단백질로 표준화하고, 레인당 총 가용성 단백질 20㎍의 표준화 적재량에 대해 40mM DTT와 함께 LDS 시료 완충제(Invitrogen, Carlsbad, CA) 중에서 제조하였다. 시료를 3-8% 트리스 아세테이트 겔(Invitrogen, Carlsbad, CA) 중에서 전기영동시키고, 니트로셀룰로즈막으로 이동시켰다. 블롯을 블록킹 완충제로 블록킹하고, 상이한 PUFA 신타제 OrfA, OrfB 및 OrfC 폴리펩티드에 대해 항체로 탐침했다. 스키조키트리움 PUFA 신타제 아단위 A(SzPUFS-A)의 A2 영역에 대해 지시된 래빗 항-A2-A 및 스키조키트리움 PUFA 신타제 아단위 B(SzPUFS-B)의 B3 영역에 대해 지시된 래빗 항-B3-A를 사용하였다. 영역 B3은 에노일 리덕타제(ER) 영역이다. 아단위 C에 또한 ER 영역이 존재하고, 따라서 이 항혈청은 웨스턴 블롯 상에서 아단위 B 및 C 모두 인지한다. 항-래빗 형광성 표지된 2차 항체(염소 항-래빗 AF 633 (Invitrogen, Carlsbad, CA))를 검출용으로 사용하였다. 블롯을 타이푼 트리오 플러스 형광성 영상화기(Typhoon Trio Plus fluorescent imager)(GE Healthcare, New Brunswick NJ) 상에서 시각화하였다.
이벤트 5197[14]-032.002로부터 T1 종자를 개발하는 최후 단계(>30 DAP)로부터의 추출물의 SDS-PAGE 웨스턴 블롯은 OrfA, OrfB 및 OrfC 특이적 항혈청으로 탐침될 경우 적합한 크기에서 밴드를 나타냈다(도 6). 이러한 밴드들은 또한 쿠마시에 블루에 의한 직접 염색으로 제시될 수 있다. OrfA, OrfB 및 OrfC는 또한 DHA 생성 이벤트 5197[13]-010.001, 5197[21]-052.001, 5197[21]-053.001 및 5217[6]-065.002로부터 종자 시료에서 검출되었다.
DHA 생성 캐놀라 이벤트 5197[14]-032.002.Sx002로부터 (DAP) 수분 후 15, 20, 25, 30, 35, 및 42일에 수집된 개발 T2 종자 시료 세트를 지질 함량(도 7a) 및 웨스턴 블롯에 의한 OrfA, OrfB 및 OrfC 폴리펩티드의 존재(도 7b)에 대해 분석하였다.
3개의 폴리펩티드 모두의 발현은 수분작용 후 30일 및 35일에 개발 종자에서 검출되었고, 수분 후 42일에 및 성숙된 종자에서 현저하게 검출되었다(도 7a 및 도 7b).
실시예 8
T 2 캐놀라 종자에서 DHA , DPA 및 EPA 수준
이벤트 5197[14]-032.002로부터 T1 종자를 온실에 심고, 잎 시료를 DNA 분석을 위해 4 내지 5엽 단계에서 96개 식물로부터 취해 각 T1 분리체 식물에서 유전자변형의 카피 수를 측정했다. 이는 상기한 프로토콜을 사용하여 팻 유전자의 가수분해 프로브 분석으로 수행하고, 3개의 별개의 분리체 부류: 21개 동형접합성, 45개 이형접합성 및 30개 눌 식물을 동정했다. 모든 동형접합성 및 31개 눌 식물을 온실에서 성숙기까지 성장시키고, 종자를 수거했다. 동형접합성 및 눌 식물로부터 식물당 평균 T2 종자 수율은 각각 7.36gm 및 8.61gm이었다.
이벤트 5197[14]-032.002의 온실-성장된 T1 식물로부터 T2 종자의 장쇄 다중불포화 지방산(LC-PUFA) 함량은 상기한 바와 같이 GC-FAMA 분석에 의해 8 내지 12개 종자의 대량 추출로 측정했다. 동형접합성 식물의 LC-PUFA 함량은 도 8에 제시되어 있다. 어떠한 LC-PUFA도 임의의 눌 분리체의 종자에서 검출되지 않았다. 20개의 유전자변형 라인은 대량 종자 분석에서 0.28% 내지 0.90% DHA를 생산했고, 하나의 라인은 어떠한 LC-PUFA도 생산하지 못했다. 또한, DHA 함유 종자는 0.09 내지 0.34% DPA(n-6)을 생산했다. 총 PUFA(DHA+DPA) 중의 DHA의 평균 비율은 77%였다.
0.7% 초과의 DHA를 생산하는 4개 라인으로부터 종자의 지방산 조성은 4개 눌 분리체 라인의 조성과 비교하여 표 13에 제시되어 있다.
Figure pct00013
이들 동형접합성 T1 식물(4, 35, 63, 96, 50 및 106)의 6개 라인으로부터 48 T2 종자의 단일 종자 분석을 수행했다. GC-FAME 프로파일의 상세 분석은, 추가 피크가 DHA 및 DPA를 함유하는 종자에 일정하게 존재함을 나타냈다. 이는 인증 표준(NU-Chek)과 비교하여 C20:5(n-3) EPA로 동정되었다. 체류 시간은 인증 EPA(C20:5(n-3))의 것과 합치했고, PolarisQ에 의한 GC-MS로 측정한 공칭 분자량은 동일했다.
6개 라인으로부터 단일 T2 종자의 LC-PUFA의 요약은 도 9에 제시되어 있다. DHA 함량이 1.6% 이하인 단일 종자가 발견되었다. 또한, EPA 함량이 0.27%인 식물이 동정되었다.
상반 교잡을 2개의 T1 라인과 비형질전환 넥세라710 사이에서 수행했다. 수득된 모 종자 및 F1 하이브리드 종자를 DHA 함량에 대해 분석했다(도 10). 도 10에서, 다이아몬드는 X 축 상에 기재된 각 범주에 대한 평균을 나타낸다. 수직 막대는 당해 범주에 대한 평균을 나타내고, 다이아몬드 극단 사이의 거리는 95% 신뢰 구간이다. F1 종자(0.29% 및 0.28%)에서 평균 DHA 수준은 유전자변형 모 종자가 축적하는 것(0.51% 및 0.47%)의 절반이다. 표현형과 접합자성 수준의 정량적 상관은 이 결과로부터 추정될 수 있다.
요약하면, 이들 데이터는 5개 유전자변형에 의해 제공된 DHA 형질이 유전가능하고 2대(second generation)에서 유지됨을 나타낸다.
실시예 9
캐놀라 이벤트-10 T2 종자에서 DHA 생산
캐놀라 이벤트 5197[13]-010.001(도 11에 제시된 팻 유전자의 2 카피를 함유)로부터의 60개 T1 종자를 온실에 심었다. 팻 유전자의 가수분해 프로브 분석은 팻 유전자의 0 내지 4개 카피에 상응하는 분리체의 5개 독특한 부류를 동정했다.
유전자변형 삽입체에 상응하는 2개 유전자좌는 서던 블롯 분석(유전자좌 A 및 B를 나타냄)에 의해 구별될 수 있다. 2개의 팻 카피를 함유하는 모든 식물로부터의 DNA를 서던 블롯으로 분석하여 이들의 유전자형(유전자좌 A 또는 위치 B에 대해 동형접합성, 또는 두 위치에 대해 이형접합성)을 결정할 수 있다. 4개의 단일 카피 및 2개의 눌 대조군 식물을 대조군으로서 또한 분석했다. 종자를 수거하고, LC-PUFA 함량에 대해 대량 종자 분석으로 분석했다(표 14).
Figure pct00014
이들 데이터는 이벤트 5197[13]-010.001 유전자좌 A에서 동형접합성인 식물이 LC-PUFA의 생산을 유도하는 반면 유전자좌 B 동형접합체는 유도하지 않음을 나타낸다. 추가로, 유전자좌 B는 3-카피 식물이 생산하는 것과 같이 4-카피 이중 동형접합체가 매우 낮은 수준의 DHA를 생산하기 때문에 LC-PUFA를 간섭한다. 유사하게는, 이형접합성 단일-카피 유전자좌 A 식물은 0.47% LC-PUFA를 생산하는 반면, 이형접합성 단일-카피 유전자좌 B는 매우 낮은 수준의 LC-PUFA(0.02%)를 생산한다.
유전자좌 A(및 유전자좌 B의 경우 눌)에서 동형접합성인 이벤트 5197[13]-010.001로부터 유래된 식물로부터 대량 T2 종자의 GC-FAME 분석에 의해 측정된 완전 지방 조성은 표 15에 제시되어 있다.
Figure pct00015

실시예 10
캐놀라에서 DHA 의 필드 생산
최고 수준의 DHA를 함유하는 5197[14]-032.002의 10개 동형접합성 라인으로부터 T2 종자를 풀링하여 60gm의 종자를 수득했다. 종자는 또한 네거티브 대조군으로 사용하기 위한 47gm의 종자를 수득하기 위해 10개 눌 분리체 라인으로부터 풀링했다. 종자를, 각 구획에서 8개 플롯(plot)에 변형유전자 함유 종자, 6개 플롯에 눌 분리체 종자 및 2개 플롯에 시판 대조군(넥세라 845CL)와 함께, 2009년 5월에 노쓰 다코다(North Dakota)에서 2개 구획에 심었다. 모든 유전자변형 식물 플롯 및 4개의 눌 분리체 플롯은 개화 동안 격리 케이지로 덮었다. 나머지 2개 눌 플롯 및 넥세라 845CL 플롯은 덮지 않은 상태로 유지했다. 플롯을 감싸고, 표준 실시에 따라 9월에 수확했다. 부위 1에서, 플롯 평균 0.95kg의 종자를 유전자변형 식물 및 0.99kg을 눌 식물로부터 수득했다. 부위 2에서, 플롯 평균은 유전자변형 식물에서 0.64kg이었고, 눌 식물에서 0.73kg이었다. 각 플롯으로부터 종자의 GC-FAME를 수행하여 필드 성장 종자에서 LC-PUFA의 수준을 측정했다(표 16).
Figure pct00016
표 16의 결과는 각 플롯으로부터 3개 시료의 분석을 나타낸다. 플롯 1 내지 11로부터의 종자는 다른 부위 플롯(평균 76.7% 18:1 및 2.9% 18:3)과 비교하여 보다 낮은 수준의 18:1(65.5%) 및 보다 높은 수준의 18:3(7.6%)를 함유했고, 따라서 통상의 캐놀라로 광범위하게 오염된 것으로 간주되었다. 이 플롯은 후속 분석으로부터 배제했다. 부위 1로부터 유전자변형 식물로부터 T3 종자의 10개 종자 벌크 분석에 의한 평균 DHA 함량은 0.19%이었고, 부위 2로부터의 함량은 0.26%였다. 최고 DHA 함량은 0.38%(0.03% EPA와 함께). n-3 LC-PUFA/총 PUFA의 평균 비율(%)은 73%였다.
필드 시험에 사용된 각 T2 라인의 시료를 또한 온실에서 성장시켰다. T3 온실 종자의 10개 종자 벌크 분석에 의한 평균 DHA 함량은 0.22%였고, 각각의 식물은 0.8% 이하의 DHA를 가졌다. 이는 필드에서 생산된 DHA의 양과 상관된다.
이들 데이터는 당해 PUFA 신타제 유전자 쉬트가 필드 조건하에 DHA의 생산을 유도할 수 있음을 나타낸다.
실시예 11
마이크로어레이 기술을 사용한 DHA 유전자 발현 분석
캐놀라 종자의 발달을 수분 후 15, 20, 25, 30, 35 및 42일(DAP)에서 유전자변형 동형접합성 이벤트 5197[14]-032.002 라인 및 비형질전환된 눌 식물로부터 수집했다. 단색 구형 유전자 발현 프로파일링 설계를 사용하여, 종자 발달 동안 규정된 시점 각각에 대해 비형질전화된 눌 라인에 대한 동형접합성 형질전환 라인으로 새롭게 도입된 유전자 각각의 발현 수준을 측정했다. 개개 60-머 올리고 배열의 3개의 동일한 기술적 복사체(Agilent Technoligies Inc., Santa Clara, CA)를 각 시료로부터 증폭된, Cy3 표지된 cRNA와 하이브리드화시켰다. 통상의 설계된 (eArray, Agilent Technologies Inc., Santa Clara, CA) 60-머 포괄 전사체 전체의 캐놀라 올리고뉴클레오티드 배열을 사용하여 상기한 하이브리드화를 수행했다. 이러한 배열은 공중 데이터 공급원으로부터 수득한 37,000개 이상의 상이한 캐놀라 전사체(Agilent Technologies Inc., Santa Clara, CA)를 함유한다. 각 전사체의 발현 수준을 효율적으로 측정하기 위해, 당해 배열에 존재하는 올리고는 각 표적이 예상된 표적 서열과 효율적으로 하이브리드화하도록 독특하고 특이적으로 설계했다. 하나 이상의 전사체와 이중쇄를 형성하는 올리고는 당해 배열로부터 제거했다. 각 올리고는 또한 마이크로어레이 처리 전체에 걸쳐 최적 성능에 필요한 화학적 및 물리적 특성을 충족시킨다. 또한, 새롭게 도입된 유전자 뿐만 아니라 목적하는 몇몇 기타 유전자를 나타내는 특이적 및 독특한 올리고는 또한 통상의 설계된 캐놀라 올리고 배열에 제시되어 있다. 60-머 올리고는 제조업자로부터 슈어-프린트(Sure-Print) 기술을 사용하여 동일 반응계에서 합성했다.
RNA 분리 및 정제
이벤트 5197[14-032.002 및 눌 식물 대조군으로부터의 종자를 발달시키는 시료를 동결시키고, 풀링하여 RNA 분리 및 정제용 출발 물질로서 사용했다. 풀링된 시료당 종자 조직 총 500mg을 막자사발 및 공이를 사용하여 액체 질소와 함께 연마하고, 연마된 조직 약 50mg을 RNA 추출용 RNeasy 키트(Qiagen, Valencia, CA)로부터 추출 완충제 RLT 450㎕에 재현탁시켰다. 시료를 단시간 와동시켜 조직을 파쇄한 다음, 추출 프로토콜을 계속했다. 총 RNA는 RNA 추출용 RNeasy 키트(Qiagen, Valencia, CA)로부터의 지침에 따라 정제했다. 이어서, 정제된 총 RNA는 NanoQuant(TECAN, Research Triangle Park, NC) 분광계를 사용하여 정량하고, 표준 1% 아가로즈 겔 전기영동에 의해 가시화했다.
표지화를 위해, 각 시료로부터 정제된 총 RNA 총 1.0㎍을 역전사시키고, 증폭시키고, 아길런트(Agilent; Santa Clara, CA) 단색 마이크로어레이 기반 유전자 발현 QuickAmp 표지화 프로토콜에 따라 Cy3-CTP로 표지했다. 각 캐놀라 배열은 관리하의 1300개 이상의 내부 스파이크를 함유하기 때문에, 키트 내의 단색 RNA 스파이크(Agilent, Santa Clara, CA)는 또한 제조업자의 지침에 따라 표지했다. 시료는 MMLV 역전사효소를 사용하여 역전사시키고, T7 RNA 폴리머라제를 사용하여 증폭시켰다. 증폭 후, cRNA는 Qiagen RNeasy 미니스핀 컬럼을 사용하여 정제하고, NanoQuant 분광계(TECAN, Research Triangle Park, NC)를 사용하여 정량했다. Cy3에 대한 특이적 활성은 하기 식에 의해 측정했다: (Cy3의 농도/(cRNA의 농도)*1000 = cRNA ㎍당 Cy3의 pmol. 하이브리드화를 위한 시료는 1.65 ㎍으로 표준화했고, 특이적 활성은 cRNA ㎍당 Cy3 9.0 pmol을 초과했다.
하이브리드화 , 스캐닝 및 특성 추출
올리고 유전자 발현 배열은 아길런트 테크놀로지(Santa Clara, CA) 유전자 발현 하이브리드화 키트 및 세정 완충제 키트를 사용하여 하이브리드화시켰다. 하이브리드화는 완전 자동화 TECAN HS4800 PRO(TECAN, Research Triangle Park, NC) 하이브리드화 스테이션에서 수행했다. 하이브리드화 혼합물을 65℃에서 주입하고, 17시간 동안 진탕과 함께 배양한 다음, 30초 동안 65℃에서 슬라이드 예비-하이브리드화 단계를 수행했다. 이어서, 슬라이드는 아길런트 GE 세정 #1을 사용하여 1분 동안 37℃에서 세정한 다음, 1분 동안 아길런트 GE 세정 #2로 30℃에서 2차 세정하고, 30℃에서 2분 30초 동안 질소 가스를 사용하여 최종 건조 단계를 수행했다. 슬라이드를 즉시 스캐닝하여, 시그널 강도에 대한 환경 산화물의 영향을 최소화했다.
배열은 SureScan 고해상도 기술(Agilent Technologies, Santa Clara, CA)와 함께 아길런트 G2565CA 마이크로어레이 스캐너를 사용하여 스캐닝했다. 각 배열을 스캐닝하는 프로토콜은 염색 채널, 스캔 영역 및 해상도, TIFF 화일 동적 범위, PMT 게인 및 최종 이미지 결과용 설정에 대한 파라미터를 규정한다. 당해 배열이 스캐닝되면, 그리드 피트의 배치 및 최적화, 스폿의 발견, 분리체의 블래그, 배경 바이어스, 에러 및 비율의 연산 및 품질 조절 계량의 계산을 위해 규정된 파라미터를 사용하여 특성 추출(FE) 프로토콜을 수행했다. 스캐닝 및 특성 추출 프로토콜을 완료한 후, 품질 조절 계량 리포트 및 모든 원 데이터를 함유하는 최종 화일(TXT)와 함께 Cy3 이미지를 함유하는 TIFF 화일을 생성한다. 이미지 화일(TIFF)을 사용하여, 슬라이드의 일반 품질, 우측 위치(4개 코너)에서 관리하의 스파이크의 존재 및 강도를 검사하고 또한 하이브리드화, 세정, 스캐닝 및 특성 추출 고정이 성공적이었는지를 확인한다. FE 품질 관리(QC) 리포트는, 아길런트 테크놀로지스(Santa Clara, CA)에 의해 제공 및 설계된 포지티브 및 네거티브(원핵생물 유전자 및 인공 서열) 관리하 스파이크에 기반한 데이터의 분산을 허용하는 편차 계수의 값을 제공한다. 이 리포트는 또한 데이터 분포, 균일성, 배경, 재생능, 감도 및 데이터의 일반 품질에 대한 정보를 제공했다. 모든 원 데이터를 함유하는 TXT 파일(file)을 추가 분석을 위해 GeneSpring(Agilent, Santa Clara, CA)에 업로드했다.
데이터 표준화 및 통계학적 분석
스캐닝 및 특성 추출 후, 원 데이터 파일을 GeneSring GX 버젼 10.0.2(Agilent Technologies, Santa Clara, CA)에 업로드하고, 각 배열 데이터 파일을 시료로서 한정하고 적절한 파라미터 값을 할당하는 프로젝트를 생성했다. 동일한 파라미터 값을 갖는 시료는 복사체로서 처리했다. 해석은 당해 시료가 실험 조건으로 어떻게 분리되고 데이터 가시화 및 분석에 어떻게 사용되었는지를 상술하기 위해 생성되었다. 상기 정의한 관리하 스파이크, 파라미터 및 해석에 기반한 시료에 대한 품질 관리는 분석 개시 전에 데이터의 품질을 보장하기 위해 수행했고, GeneSpring에 의한 품질 관리 계량 리포트를 생성했다.
데이터는 분류상 비생물학적 차이를 최소화하고 교차 비교용 배열을 표준화하기 위해 구형 백분위 이동 정규화 방법을 사용하여 표준화했다. 이러한 알고리즘은 시그널 강도를 밑이 2인 로그로 변환시키고, 이들을 증가하는 차수로 정렬하여, 75번째 백분위의 등급을 계산하고, 이 값을, 표준화 강도 값을 생성하는 로그 변환 시그널 강도 각각으로부터 뺐다. 데이터는 연구하의 모든 단일 시료에 존재로서 플래깅된(flagged) 속성을 선택하고 한계 또는 부재로서 플래깅된 속성을 제거하여 여과했다. 속성의 여과된 및 표준화된 리스트는 이원 ANOVA 방법을 사용하여 통계학적 분석용 투입으로 사용되었고, 여기서 p <0.05의 보정된 p-값 컷오프는 DAP 및 유전자형을 파라미터로서 한정한다. 새롭게 도입된 유전자 각각에 대한 발현 프로파일을 측정했다.
결과
표지된 및 증폭된 cRNA 뿐만 아니라 총 RNA의 농도에 대해 수득된 값은 최적이었다. 또한, 증폭후 농도에 대한 값, Cy3에 의한 표지 효율 및 일정한 및 신뢰가능한 결과에 필요한 특이적 활성은 우수했다. 스캐닝후 각 개개 배열에 대한 특성 추출 프로토콜에 의해 제공된 품질 관리(QC) 리포트는, 포지티브 및 네거티브 관리하 스파이크에 기반한 데이터의 분산을 측정하는데 사용된 편차 계수 값을 제공했다. 리포트로부터 수득된 모든 값은 데이터 분포, 균일성, 배경 및 감소의 최적 품질을 나타냈다. 이 연구 동안 사용된 시료에 대한 GeneSpring(Agilent Technologies, Santa Clara, CA) 품질 관리 계량 리포트는, 수득된 데이터의 재현성 및 신뢰성의 평가를 보조하는 유의적인 통계학적 값을 제공했다. 기술적으로 복사된 배열 그룹(시료당 3개)에 대한 보고된 값은 소정 범위내였고, 수득된 데이터가 신뢰가능함을 나타냈다(데이터는 도시하지 않음).
동형접합성(표 17)("DAP"는 수분 후 일수를 나타낸다) 및 눌(표 18) 라인에 대한 종자 발달 동안 한정된 6개 시점 각각에 대해 보고된 원 값은, 필요한 경우, 배경 뺄셈 및 곱셈 탈경향을 포함하여, 모든 특성 추출(FE) 처리 단계가 완료된 후에 잔류한 시그널 강도 값을 나타낸다. 한편, 동형접합성(표 19) 및 눌(표 20) 라인에 대한 표준화 값은, 기술적 편차를 설명하고 분류법상 비생물학적 차이를 최소화하고 교차 비교용 배열을 표준화하는 범용 백분위 이동 표준화 방법을 사용하여 처리했다.
Figure pct00017

Figure pct00018

Figure pct00019

Figure pct00020
종자 발달 동안 모든 시점에서 원 라인에 대해 수득된 표준화 값(도 12) 및 눌 라인에 대해 수득된 표준화(표 13) 값의 도식적 표시는, 이들 유전자가 오메가-9 넥세라 710 비형질전환된 라인에 존재하지 않고 따라서 현저한 발현이 검출되지 않음을 확증한다. 도 14(원) 및 도 15(표준화)에 제시된 이벤트 5197[14]-032.002 라인에서, 종자 발달이 진행됨에 따라 모든 유전자의 전사 축적을 증가시키는 일반적 경향을 관찰할 수 있다. 전사 축적의 초기 현저한 증가는 15 및 30 DAP 동안 발생하고, DAP 42에서 최대 수준에 도달한다. 도 14에 제시된 원 곡선은 당해 연구하에 유전자 각각에 대해 수득된 상대 하이브리드화 강도 값의 가시화를 제공하고, 도 15에 요약된 표준화 곡선은 최소화된 분류학상 비생물학적 변이 및 표준화 비교 교차 배열을 갖는 유전자 발현 프로파일의 일반 경향을 나타낸다.
실시예 12
대체 프로모터를 사용한 조류 PUFA 신타제 유전자 쉬트( Suite )
PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판토테이닐 트랜스퍼라제 HetI 단백질을 코딩하는 유전자(들)를 발현시키기 위한 추가의 전사 조절 요소의 사용은 캐놀라에서 DHA 함량을 추가로 증가시킬 수 있다. 발달시에 조기에 및 연장된 시간 동안 발현하는 전사 조절 요소의 동정 및 사용은 종자 발달의 초기 단계(예: 15 내지 25 DAP)에서 이종성 유전자의 전사를 촉진시킴으로써 캐놀라에서 DHA 수준을 증가시킬 수 있고 따라서 DHA 생산 시간을 연장시킬 수 있다. 이러한 전사 조절 영역의 예는, 이로써 한정되는 것은 아니지만, LfKCS3 프로모터(미국 특허 제7,253,337호) 및 FAE 1 프로모터(미국 특허 제6,784,342호) 및 ACP 프로모터(WO 1992/18634)를 포함한다. 이들 프로모터는 단독으로 또는 조합 사용되어, 하기 플라스미드에서 이미 기재된 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 발현 카세트의 발현을 유도한다; pDAB7361, pDAB7362, 및 pDAB7363. 플라스미드 내의 전사 조절 영역을 치환하는 방법은 당해 기술분야에 공지되어 있다. 예를 들면, PvDlec2 프로모터 v2를 포함하는 폴리뉴클레오티드 단편은 pDAB7361, pDAB7362 또는 pDAB7363(또는 선행 플라스미드는 pDAB7361, pDAB7362 또는 pDAB7363을 구성하기 위해 사용되었다)로부터 제거되고, LfKCS3 또는 FAE 1 프로모터 영역으로 대체되었다. 새롭게 작제된 플라스미드를 사용하여 캐놀라 식물을 안정하게 형질전환시킨다. 유전자변형 캐놀라 식물을 분리하고, 분자적으로 특성화한다. 수득된 LC-PUFA 축적을 측정하고, 0.01% 내지 15% DHA 또는 0.01% 내지 10% EPA를 생산하는 캐놀라 식물이 동정되었다.
pDAB9166 의 작제
pDAB9166 플라스미드(도 26; 서열번호: 46)은 다중-부위 게이트웨이 L-R 재조합 반응을 사용하여 작제했다. pDAB9166은 3개의 PUFA 신타제 PTU, 1개의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 LfKCS3 프로모터 v1, SzPUFA OrfA v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 LfKCS3 프로모터 v1, SzPUFA OrfB v3 및 AtuOrf23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 LfKCS3 프로모터 v1, hSzThPUFA OrfC v3 및 AtuORF23 3' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 LfKCS3 프로모터 v1, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB9161, pDAB9162, pDAB9163, pDAB101484 및 pDAB7333을 재조합하여 pDAB9166을 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3였다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB9167 의 작제
pDAB9167 플라스미드(도 27; 서열번호 47)은 다중-부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB9167는 3개의 PUFA 신타제 PTU, 1개의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 LfKCS3 프로모터 v1, SzPUFA OrfA v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 BoACP 프로모터 v1, BoACP 5' UTR v1, SzPUFA OrfB v3 및 AtuOrf23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 LfKCS3 프로모터 v1, hSzThPUFA OrfC v3 및 AtuORF23 3' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 BoACP 프로모터 v1, BoACP 5' UTR v1, NoHetI v3 및 AtuORF23 3' UTR v1를 함유한다.
플라스미드 pDAB9161, pDAB9165, pDAB9163, pDAB101485 및 pDAB7333를 재조합하여 pDAB9167를 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3였다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB7379 의 작제
pDAB7379는 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. SzACS-2 유전자 서열은 이 작제물에 포함되지 않는다. pDAB7379 플라스미드(도 28; 서열번호 48)은 다중-부위 입구 L-R 재조합 반응을 사용하여 작제했다.
pDAB7379는 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v3 및 AtuORF23 3' UTR v1를 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, hSzThPUFA OrfC v3 및 AtuORF23 3' UTR v1를 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB7371, pDAB7372, pDAB7373, pDAB7374 및 pDAB7333를 재조합하여 pDAB7379를 형성했다. 구체적으로, 상기한 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이었다. pDAB7333은 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB7380 의 작제
pDAB7380은 재구성된, 코돈 최적화 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. SzACS-2 유전자 서열은 이 작제물에 함유되지 않는다. 이 작제물에 사용된 파세올린의 버젼은 문헌[참조: Bustos et al ., 1989 (The Plant Cell, Vol. 1; 839-853)]에 기재된 바와 같이 실질적으로 변형시켰고, 여기서 프로모터의 5' 부분은 절단되었고, 파세올린 5' 비해독된 영역은 완전하게 잔류했다. pDAB7380 플라스미드(도 29; 서열번호 49)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다.
pDAB7380은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfA v3 및 AtuORF23 3' UTR v1를 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfB v3 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, hSzThPUFA OrfC v3 및 AtuORF23 3' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v5, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB7375, pDAB7376, pDAB7377, pDAB7378 및 pDAB7333을 재조합하여 pDAB7380을 형성했다. 구체적으로, 상기한 4개 PTU는, 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이었다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB9323 의 작제
pDAB9323은 천연, 비코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC, SzACS-2 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. pDAB9323 플라스미드(도 30; 서열번호 50)은 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다.
pDAB9323은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v2, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v2, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfC v2, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)을 함유한다. 아실-CoA 신세타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzACS-2 v2 유전자, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, NoHetI v2, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)을 함유한다.
플라스미드 pDAB9307, pDAB9311, pDAB9315, pDAB9322 민 pDAB7333을 재조합하여 pDAB9323을 형성했다. 구체적으로, 상기한 5개의 PTU는, 식물 형질전환 이원 pDAB7333의 T-스트랜드 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v2, SzPUFA OrfB v2, SzPUFA OrfC v2, NoHetI v2이었다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 6개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 6개 PTU의 도입에 대해 시험했다.
pDAB9330 의 작제
pDAB9330은 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC, SzACS-2 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. pDAB9330 플라스미드(도 31; 서열번호 51)은 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB9330은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, hSzThPUFA OrfC v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 아실-CoA 신세타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzACS-2 v3 유전자, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, NoHetI v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다.
플라스미드 pDAB9324, pDAB9325, pDAB9326, pDAB9329 및 pDAB7333를 재조합하여 pDAB9330를 형성했다. 구체적으로, 상기한 5개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, SzACS-2 v3, NoHetI v3이었다. pDAB7333은 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 6개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 6개 PTU의 도입에 대해 시험했다.
pDAB9337 의 작제
pDAB9337는, 이의 발현이 파세올린 프로모터에 의해 유도되는, 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. pDAB9337 플라스미드(도 32; 서열번호 52)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다.
pDAB9337은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, hSzThPUFA OrfC v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, NoHetI v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다.
플라스미드 pDAB9324, pDAB9325, pDAB9326, pDAB9328 및 pDAB7333를 재조합하여 pDAB9337을 형성한다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB9338 의 작제
pDAB9338은 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. 파세올린 프로모터를 사용하여 SzPUFA OrfA의 발현을 유도하고, PvDlec2 프로모터를 사용하여 기타 변형유전자를 유도한다. pDAB9338 플라스미드(도 33; 서열번호 53)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다.
pDAB9338은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1를 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB9324, pDAB7335, pDAB7336, pDAB7338 및 pDAB7333를 재조합하여 pDAB9338을 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB9344 의 작제
pDAB9344는, 모두가 코딩 서열의 아미노 말단에 융합되는 리불로스 비스포스페이트 소형 쇄 1A(SSU-TP v1으로 표지됨)를 함유하는, 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. 파세올린 프로모터를 사용하여 SzPUFA OrfA의 발현을 유도하고, PvDlec2 프로모터를 사용하여 기타 변형유전자를 유도한다.
pDAB9344 플라스미드(도 34; 서열번호 54)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB9344는 3개 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v4, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v4, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, hSzThPUFA OrfC v4, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, NoHetI v4, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)을 함유한다.
플라스미드 pDAB9343, pDAB9342, pDAB9340, pDAB9331 및 pDAB7333를 재조합하여 pDAB9344를 형성했다. 구체적으로, 상기한 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v4, SzPUFA OrfB v4, hSzThPUFA OrfC v4, NoHetI v4이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 6개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB9396 의 작제
pDAB9396은 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC, SzACS-2 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. 파세올린 프로모터를 사용하여 SzPUFA OrfA 및 SzPUFA OrfB의 발현을 유도한다. PvDlec2 프로모터를 사용하여 다른 변형유전자: hSzThPUFA OrfC, SzACS-2 및 NoHetI을 유도한다.
pDAB9396 플라스미드(도 35; 서열번호 55)는 다중 부위 L-R 재조합 반응을 사용하여 작제했다. pDAB9396은 3개 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1를 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 아실-CoA 신세타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzACS-2 v3 유전자, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB9324, pDAB7335, pDAB7336, pDAB7339 및 pDAB7333을 재조합하여 pDAB9338을 형성했다. 구체적으로, 상기한 PTU는 식물 형질전환 이원 pDAB733의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, SzACS-2 v3, NoHetI v3이다. pDAB7333은 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 6개 PTU의 도입에 대해 시험했다.
pDAB101412 의 작제
pDAB101412는 재구성된, 코돈 최적화된 버젼의 SzPUFA OrfA, SzPUFA OrfB, hSzThPUFA OrfC, SzACS-2 및 NoHetI을 함유하도록 작제한 이원 플라스미드이다. 이 작제물에 사용된 파세올린 프로모터의 버젼은 문헌[참조: Bustos et al ., 1989 (The Plant Cell, Vol. 1; 839-853)]에 기재된 바와 같이 실질적으로 변형시켰고, 여기서 프로모터의 5' 부분은 절단되었고, 파세올린 5' 비해독된 영역은 완전하게 잔류했다. 절단된 파세올린 프로모터 서열은 본원 전반에 걸쳐 버젼 4(v4), 버젼 5(v5) 및 버젼 6(v6)으로 동정되었다. pDAB101412 플라스미드(도 36; 서열번호 56)은 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다.
pDAB101412는 3개 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfA v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfB v3 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, hSzThPUFA OrfC v3 및 AtuORF23 3' UTR v1을 함유한다. 아실-CoA 신세타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, 2S 5' UTR, SzACS-2 v3 유전자 및 AtuORF23 5' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v5, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB7375, pDAB7376, pDAB7377, pDAB7398 및 pDAB7333을 재조합하여 pDAB101412를 형성했다. 구체적으로, 상기한 5개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, SzACS-2 v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 6개 PTU의 도입에 대해 시험했다.
종자 발달에서 조기에 발현시키는 프로모터를 사용한 캐놀라 형질전환
플라스미드를 사용하여, 상기한 프로토콜을 사용하여 캐놀라 식물을 안정하게 형질전환시킨다. 유전자변형 캐놀라 식물을 분리하고, 분자적으로 특성화한다. 대체 작제물의 사용은 보다 많은 양의 DHA 및 LC-PUFA를 함유하는 캐놀라 식물을 생성한다. 수득된 LC-PUFA 축적을 측정하고, 0.01% 내지 15% DHA 또는 0.01% 내지 15% LC-PUFA를 생산하는 캐놀라 식물을 동정한다.
실시예 13
캐놀라에서 조류 PUFA 신타제 유전자 쉬트에 의한 DGAT2 또는 ACCase 의 공-발현
캐놀라 식물 내의 오일 함량은 아세틸 CoA 카복실라제(ACCase) 또는 타입 2 디아실글리세롤 아실트랜스퍼라제(DGAT2)를 코딩 및 발현시키는 키메라 DNA 분자의 형질전환에 의해 추가로 변형시킨다. 이들 유전자는, PUFA 신타제 유전자를 함유하는 캐놀라 식물로 ACCase 또는 DGAT2 발현 카세트를 함유하는 캐놀라 식물을 육종하거나 ACCase 또는 DGAT2 및 PUFA 신타제 유전자를 함유하는 유전자 스택(stack)으로 캐놀라 식물을 형질전환시킴으로써, 상기한 조류 PUFA 신타제 유전자로 공-발현시킨다. ACCase 또는 DGAT2 코딩 서열의 발현에 필요한 조절 요소는 상기한 것들을 포함할 수 있다. 당해 기술분야에 공지된 추가의 조절 요소 발현 서열을 또한 사용할 수 있다. ACCase 및 DGAT2 발현 카세트는 상기한 형질전환 프로토콜을 사용하여 캐놀라로 형질전환시킨다. 형질전환은 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 발현 카세트와 조합된 ACCase 또는 DGAT2 발현 카세트의 분자 스택으로서; 또는 선택가능한 마커에 연결된 다음, PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 발현 카세트를 함유하는 캐놀라 식물과 가교된 독립적 ACCase 또는 DGAT2 발현 카세트로서 발생할 수 있다. 포지티브 형질전환체를 분리하고, 분자적으로 특성화한다. 비형질전환된 대조군 캐놀라 식물과 비교하여, 식물, 식물 종자 또는 식물 오일 농축물에서 LC-PUFA의 증가된 축적을 함유하는 캐놀라 식물을 동정한다. 이러한 증가는 1.2배 내지 20배 증가 범위일 수 있다.
세포질에서 ACCase의 과발현은 보다 높은 수준의 말로닐-CoA를 생산할 수 있다. 증가된 수준의 세포질 말로닐-CoA를 함유하는 캐놀라 식물 또는 종자는, 조류 PUFA 신타제 유전자가 존재하여 발현되는 경우, 후속적으로 보다 높은 수준의 장쇄 다중불포화 지방산(LC-PUFA)를 생산할 수 있다. 캐놀라 식물 내에서 발현되는 DGAT2 유전자는 상당량의 도코사헥사엔산(DHA) 및 에이코사펜타엔산(EPA)을 트리아실글리세롤 내로 우선적으로 도입할 수 있다. LC-PUFA에 대해 기질 선호를 갖는 DGAT2 유전자(예: WO 2009/085169)는 이들 지방산의 트리아실글리세롤(TAG) 내로의 도입을 증가시킬 수 있다. 이러한 DGAT 유전자는 LC-PUFA, 특히 DHA의 TAG 내로의 도입을 유도하고 식물 및 기타 생물체에서 TAG의 생산을 증가시키는데 유용하다.
실시예 14
식물에서 보다 높은 수준의 아실- CoA 신세타제 발현을 위한 천연 아실- CoA 신세타제 유전자 서열의 사용
스키조키트리움 종으로부터 대체 버젼의 아실-CoA 신세타제 유전자는 천연 유전자 서열을 변형시켜 과잉 개방 판독 프레임을 제거함으로써 생성했다. 이러한 버젼은 "SzACS-2 v4"로서 표지하고, 서열번호 34로서 수록했다. 당해 서열은 서비스 제공자, DNA 2.0(Menlo Park, CA)에 의해 합성했다. 코딩 서열은 프로모터 및 3' 비해독된 영역을 함유하는 식물 발현 카세트 내로 도입했고, 이는 이들 실시예에 기재되었다. 수득된 발현 카세트를 사용하여, "SzACS-2 v3", 서열번호 9로서 상기한 아실-CoA 신세타제 발현 카세트를 대체하고, 이를 PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC 및 4' 포스포판테테이닐 트랜스퍼라제 HetI 발현 카세트와 조합하여 pDAB7361, pDAB7362 및 pDAB7363을 작제했다. "SzACS-2 v4" 발현 카세트를 함유하는 새로운 플라스미드는 독특한 동정 표지를 제공했다. 새롭게 작제한 플라스미드를 사용하여 캐놀라 식물을 안정하게 형질전환시킬 수 있다. 유전자변형 캐놀라 식물을 분리하고, 분자적으로 특성화한다. 대체 버젼의 유전자, "SzACS-2 v4"는 보다 많은 양의 DHA 및 LC-PUFA를 함유하는 캐놀라 식물을 생성할 수 있다. 수득된 LC-PUFA 축적을 측정하고, 0.01% 내지 15% DHA 또는 0.01% 내지 10% EPA를 생산하는 캐놀라 식물을 동정한다.
실시예 15
성숙 유전자변형 캐놀라 종자에서 PUFA 신타제 활성
PUFA 신타제 활성은 아그로박테리움 벡터 pDAB 7361(이벤트 5197[14]-032)을 사용하여 생성한 식물로부터 성숙 T1 유전자변형 캐놀라 종자로부터의 추출물에서 검출되었다. 종자를 물에 3 내지 4시간 동안 침지시킨 다음, 종자 피막을 제거하고 추출 완충제(200 mM 포스페이트 pH 7.0, 1 mM EDTA, 1 mM DTT, 50 mM NaCl, 5% 글리세롤, 1% PVPP, 0.52 ㎍/mL 항단백독, 0.58 ㎍/mL 류펩틴, 0.83 ㎍/mL 펩스타틴 A, 12 ㎍/mL TLCK, 12 ㎍/mL TPCK, 및 6 ㎍/mL 대두 트립신 억제제)에서 드라이 아이스 상에서 연마하고, 4℃에서 10분 동안 원심분리했다. 지방 패드를 제거하고, 생성 펠렛을 보다 높은 이온 강도 완충제와 함께 배양한 다음, 재원심분리했다. 지방 패드 및 지질 층을 시료로부터 제거하고, 수성 상청액을, 50 mM 포스페이트 pH 7.2, 1 mM DTT, 10% 글리세롤 및 1 mM EDTA로 예비 평형된 제바 탈염 칼럼을 통해 통과시켰다. 비형질전환된 넥세라 710 종자를 네거티브 대조군으로서 나란히 처리했다. 두 종자 세트로부터의 시료는 HIP 추출 및 문헌[참조: Metz et al., Plant Physiol. Biochem . 47:6 (2009)]에 기재된 TLC 방법을 사용하여 분석했다(도 16). 분석 조건을 변형시켜, 2 mM NADH, NADH 재생 시스템(글루코즈 + 글루코즈 데하이드로게나제), 연속 진탕 및 최종 말로닐-CoA 농도의 100 μM(분석당 0.064 μCi/100μL)를 포함한다. 생성 상청액의 분석은 용적으로 표준화했고, FFA 제형은 60분 후에 검출될 수 있음을 나타냈다. 이는 넥세라 710 대조군에서 관찰되지 않았고, FFA 제형은 PUFA 신타제에 의해 DHA 제형으로부터 유래했음을 나타낸다.
실시예 16
공-발현된 HetI 에 의해 캐놀라에서 생산된 OrfA 의 판테테이닐화
OrfA는 9개 아실-캐리어 단백질 도메인을 함유하고, 이는 작용성이 되도록 포스포판테테이닐 트랜스퍼라제(PPTase)에 의해 포스포판테테인 그룹을 사용한 유도체화를 각각 필요로 한다. 유전자변형 캐놀라 종자에서 PPTase HetI에 의한 OrfA의 판테테이닐화 정도는 다양한 OrfA 시료로부터 판테테이닐화 부위를 함유하는 트립신 펩티드의 나노-액체 크로마토그래피-질량 분광학(나노LC-MS) 평가에 의해 평가했다.
재조합 홀로 및 아포 OrfA 폴리펩티드 표준은 HetI의 존재 또는 부재하에 공-발현에 의해 이. 콜라이에서 생산했다. HetI의 부재하에 OrfA의 발현은 비-작용성 단백질을 생성하는데, 이는 이. 콜라이로부터의 내인성 PPTase가 포스포판테테인 그룹을 부가할 수 없기 때문이다[참조: Hauvermale et al., Lipids 41:739-747; 2006]. 대조적으로, HetI에 의한 발현은 고도의 판테테이닐화를 갖는 OrfA 단백질을 제공한다. 이. 콜라이 발현된 OrfA를 추출하기 위해, 재조합 세포 배양물 0.5L로부터의 동결된 세포를 20 mL의 추출 완충제: 20 mM 트리스 pH 7.0, 1 mg/mL 리소자임, 1 mM EDTA, 1 mM PMSF, 1 mM DTT, 0.52 ㎍/mL 항단백독, 0.58 ㎍/mL 류펩틴, 0.83 ㎍/mL 펩스타틴 A, 12 ㎍/mL TLCK, 12 ㎍/mL TPCK, 6 ㎍/mL 대두 트립신 억제제에서 재현탁시켰다. 용해 후, 추출물은 DNase 및 4 mM MgCl2로 처리하고, 원심분리에 의해 정화하고, 상청액을 -80℃에서 동결시켰다.
식물 생산된 OrfA는 캐놀라 생산된 PUFA 신타제의 시험관내 분석을 위해 상기한 추출 방법을 사용하여 이벤트 5197[14]-032.002의 재수화된 성숙 캐놀라 종자로부터 분리했다. 이. 콜라이 표준 및 캐놀라 시료 둘 다로부터의 OrfA 단백질을 효소에 의해 소화시키고, 아길런트 QTOF 질량 분광계(모델 6530)에 의한 MS 분석과 함께 아길런트 칩큐브 나노크로마토그래피 유입을 사용하여 나노LC-MS에 의해 분석했다. QTOF를 프로그래밍하여 자동화 MS2 분석을 사용하여 크로마토그래피 동안 펩티드 서열 데이터를 생성했다. 당해 방법의 필수 특징은, 질량 분광계를 프로그래밍하여 전체-스캔 MS 스캔을 수행한 다음 3개의 가장 풍부한 이온의 자동화 MS2를 사용하여 MS2 서열 스펙트럼을 생성하는 것이다. 이온은 후속적으로 2회 발생 후에 MS2로부터 30초의 배제 기간 동안 배제했다. 내부 기준은 나노분무 동안 연속적으로 주입하여 QTOF의 내부 교정에 대한 기준 이온을 생성했다(m/z 299.29446 및 1221.99064에서). 보정 스톡의 이월로부터 통상 발견된 이온은 배제된 이온으로서 규정하여, 이들 이온의 허수 MS2 스캔을 방지했다. MS 스캔은 m/z 295-2400의 범위에 걸쳐 이월했다. MS2 스캔은 m/z 59-3000의 범위에 걸쳐 이월했다. 자동화 MS2를 수행하여 하기 순서로 하전 상태에 대한 선호를 제공했다: +2 > +3 > (> +3) > 미공지 > +1.
직렬 질량 스펙트럼은 마스코트 증류기(Matrix Science, London UK; 버젼 2.3.2)에 의해 추출했다. 하전 상태 디컨볼루션 및 탈동위체화는 수행하지 않았다. 모든 MS/MS 스펙트럼은 마스코트(Matrix Science, London, UK; 버젼 2.2.06) 및 X! 탄뎀(Tandem)(www.thegpm.org; 버젼 2007.01.01.1)을 사용하여 분석했다. 마스코트 및 X! 탄뎀은 트립신 소화 특이성을 가정하는 OrfA 단백질의 전체 서열을 함유하는 단백질 서열 데이터베이스를 검색하도록 설정했다. 마스코트 및 X! 탄뎀은 0.30 Da의 단편 이온 질량 내성 및 10.0 ppm의 모 이온 내성으로 검색했다. 메티오닌의 산화 및 세린의 포스포판테테인은 가변 변형태로서 마스코트 및 X! 탄뎀에서 상술되었다.
스캐폴드(버젼 Scaffold_2_05_02, Proteome Software Inc., Portland, OR)를 사용하여, MS/MS 기반 펩티드 및 단백질 동정을 확증했다. 펩티드 동정은, 이들이 펩티드 프로펫 알고리즘에 의해 상세된 바와 같이 85.0% 초과의 가능성에서 확립되는 경우에 수용되었다[참조: Keller et al., Anal . Chem. 74:5383-92 (2002)]. 단백질 동정은, 이들이 99.0% 이상의 가능성에서 확립되고 적어도 2개의 동정된 펩티드를 함유하는 경우에 수용되었다. 단백질 가능성은 프로테인 프로펫 알고리즘에 의해 지정되었다[참조: Nesvizhskii, Anal Chem. 75:4646-58 (2003)]. 유사한 펩티드를 함유하고 MS/MS 분석 단독에 기반하여 차별화될 수 없는 단백질은 최절약 원리를 충족시키도록 분류되었다. 데이터베이스 검색은 판테테이닐화 부위 1(서열번호 78 TGYETDMIEADMELETELGIDSIK) 및 판테테이닐화 부위 2-9(서열번호 77 TGYETDMIESDMELETELGIDSIK)의 아포(apo) 형태에 상응하는 트립신 펩티드를 동정했다. 판테테이닐화된 펩티드에 대한 직접 증거는 관찰되지 않았다.
캐놀라로부터 분리된 OrfA에서 부위 2-9의 판테테이닐화 정도를 평가하기 위해, 아포2-9 펩티드 양을 OrfA 분자의 다른 영역으로부터 동정된 6개의 상이한 기준 펩티드와 비교하여 측정했다(표 21).
Figure pct00021
이. 콜라이 유도된 단백질(HetI 부재)에서 기준 펩티드에 대한 아포2-9 펩티드의 내부 비율은 비판테테이닐화의 평가치로서 취했고, HetI으로 발현된 이. 콜라이 유도된 단백질에서 내부 비율은 고도의 판테테이닐화의 평가치로서 취했다. 이들 내부 비율은 기준 펩티드의 몰 풍부함이 OrfA 단백질 공급원에 상관없이 동등함을 가정한다(도 17). 6개 기준 펩티드 각각에 대한 아포2-9 펩티드의 비율을 계산하고 평균했다. (3개 비율은 6개 기준 펩티드에 대해 계산되었다). 또한, 서로에 대한 6개 기준 펩티드의 3개 비율을 계산(ref1/ref2, ref3/ref4 및 ref5/ref6)하여, 기준 펩티드가 3개 OrfA 시료 사이에 유의적으로 상이하지 않고(도 17) 존재하는 아포2-9 펩티드의 상대량을 계산하는데 적합할 수 있음을 입증했다.
기준 펩티드에 대한 계산된 비율과 대조적으로, 기준 펩티드 각각에 대한 아포2-9의 비율은, HetI의 부재하에 OrfA 표준과 비교하여, OrfA/HetI 및 캐놀라 시료 둘 다에서 아포2-9 펩티드의 수준이 현저히 낮았음을 나타냈다(도 18). 이들 결과의 가장 간단한 설명은 아포2-9 펩티드에 대한 판테테이닐화 부위가 포스포판테테이닐 그룹에 의해 실질적으로 점유되고, 이에 의해 아포2-9 펩티드의 몰 풍부함을 현저히 감소시킨다는 것이다. 이는 캐놀라 발현된 PPTase, HetI이 유전자변형 캐놀라 종자에서 OrfA를 작용적으로 활성화시킬 수 있고, 캐놀라 발현된 OrfA ACP 단위가 작용적으로 활성적임을 나타낸다.
실시예 17
추가 작제물
조절 요소의 복제를 감소시키기 위한 프로모터 다양성의 도입
유전자 침묵은 유전자변형 캐놀라 이벤트의 후대에서 관찰된 현상이다. 몇몇 조사 문헌은 문헌[참조: Waterhouse et al ., 2001 (Nature 411:834-842), Vaucheret and Fagard, 2001 (Trends in Genetics 17(1):29-35, and Okamoto and Hirochika, 2001 (Trends in Plant Sci. 6 (11): 527-534]의 것과 같은 전사 유전자 침묵(TGS) 및 전사후 유전자 침묵(PTGS)를 언급한다. 식물에서, 유전자 침묵은 변형유전자 폴리뉴클레오티드 서열의 복제(탠덤 반복 변형유전자 서열, 전환 반복 변형유전자 서열, 또는 염색체 내로의 다중 삽입)에 의해, 또는 표적 유전자 사열에 상동성인 서열이 감염 식물 바이러스 또는 아그로박테리움 투멘파시엔스의 T-DNA에 의해 운반되는 경우에 유발될 수 있다.
또한, 변형유전자 폴리뉴클레오티드 서열의 복제는 작제물 불안정성에 대한 유발원으로서 작용할 수 있다. 고도의 서열 유사성을 공유하는 다중 변형유전자 서열은 서로 다시 중첩할 수 있다. 재배열은 상동성 재조합을 통해 발생할 수 있고, 여기서 DNA의 간섭 서열은 분해된다. 그 결과, 반복된 변형유전자 폴리뉴클레오티드 서열 사이에 위치된 DNA의 단편이 분해된다.
플라스미드 벡터를 설계하는데 있어서 한 가지 전략은, 각 변형유전자의 고도 발현을 유도하는 다중, 독특한 종자 특이적 프로모터를 도입함으로써 작제물 내로 프로모터 다양성을 도입하는 것이다. 플라스미드 벡터 내로 프로모터 서열 다양성의 도입은 유전자 침묵을 감소시키고 플라스미드 안정성을 개선시킬 수 있다. 다중 종자 특이적 프로모터는 PvDlec2, 파세올린 및 Napin(미국 특허 제5,608,152호)를 포함한다. 이들 프로모터는 프로모터 활성, 예를 들면, 조직 특이성, 발현 수준, 발현 기간 등에서 상대적으로 필적한다.
pDAB7733 의 작제
pDAB7733 이원 플라스미드(도 37; 서열번호 57)은 다중-부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB7733은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfA v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 BnaNapinC 프로모터 v1, BnaNapinC 5' UTR, SzPUFA OrfB v3 및 BnaNapinC 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v5, PvPhas 5' UTR, NoHetI v3 및 AtuOrf23 3' UTR v1을 함유한다.
플라스미드 pDAB7375, pDAB7731, pDAB7336, pDAB7378 및 pDAB7333을 재조합하여 pDAB7733을 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB7734 의 작제
pDAB7734 이원 플라스미드(도 38; 서열번호 58)은 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB7734는 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfB v3 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 BnaNapinC 프로모터 v1, BnaNapinC 5' UTR, hSzThPUFA OrfC v3 및 BnaNapinC 3' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB7334, pDAB7376, pDAB7732, pDAB7338 및 pDAB7333을 재조합하여 pDAB7734를 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB101493 의 작제
pDAB101493 이원 플라스미드(도 39; 서열번호 59)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB101493은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, SzPUFA OrfB v3 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v5, PvPhas 5' UTR, NoHetI v3 및 AtuOrf23 3' UTR v1을 함유한다.
플라스미드 pDAB7334, pDAB7376, pDAB7336, pDAB7378 및 pDAB7333을 재조합하여 pDAB101493을 형성했다. 구체적으로, 상기한 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB109507 의 작제
pDAB109507 플라스미드(도 40; 서열번호 60)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB109507은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3 및 PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 BnaNapinC 프로모터 v1, BnaNapinC 5' UTR, SzPUFA OrfB v3 및 BnaNapinC 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 BoACP 프로모터/5' UTR v1, NoHetI v3 및 AtuOrf23 3' UTR v1을 함유한다.
플라스미드 pDAB9324, pDAB7731, pDAB7336, pDAB101485 및 pDAB7333을 재조합하여 pDAB109507을 형성했다. 구체적으로, 상기한 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 유소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB109508 의 작제
pDAB109508 플라스미드(도 41; 서열번호 61)은 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB109508은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfA v3 and 및 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 BnaNapinC 프로모터 v1, BnaNapinC 5' UTR, SzPUFA OrfB v3 및 BnaNapinC 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB9324, pDAB7731, pDAB7336, pDAB7338 및 pDAB7333을 재조합하여 pDAB109508을 형성했다. 구페적으로 상기한 4개 PTU은 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB109509 의 작제
pDAB109509 플라스미드(도 42; 서열번호 62)는 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB109509는 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 BoACP 프로모터/5' UTR v1, NoHetI v3 및 AtuOrf23 3' UTR v1을 함유한다.
플라스미드 pDAB7334, pDAB7335, pDAB7336, pDAB101485 및 pDAB7333를 재조합하여 pDAB109509를 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
긴 유전자 서열의 단편화를 감소시키기 위한 이원 작제물 PTU 의 순서 재배열
SzPUFA OrfA PTU를 이원 작제물의 3' 말단에 위치시켜, PTU 카세트의 순서가 분리된 유전자변형 이벤트에서 단편화 및 재배열을 감소시킬 수 있는지를 시험했다. SzPUFA OrfA는 9개 탠덤 아실 캐리어 단백질 반복체를 함유하는 거대 개방 판독 프레임(~8,700 b.p.)이다. 완성된 작제물의 제1 계열에서, SzPUFA OrfA PTU은 먼저 식물 염색체 내로 통합되도록 위치시켰다. 이어서, SzPUFA OrfA PTU는 이들의 분자 크기가 저하됨에 따라, 잔류하는 PUFA 합성 관련된 유전자 PTU를 수반했다. SzPUFA OrfA 코딩 영역의 분자 분석은 일부 유전자변형 캐놀라 및 아라비돕시스 탈리아나 이벤트가 단편화된 삽입체를 함유함을 나타냈다. 대체 작제물 설계가 기재되며, 여기서 PUFA 신타제 PTU의 순서는 하기 배열로 변경되었다: hSzThPUFA OrfC PTU, SzPUFA OrfB PTU, NoHetI PTU, SzPUFA OrfA PTU 및 PAT PTU. 이원 작제물 상에서 SzPUFA OrfA PTU의 위치 변경을 완성하여 분리된 유전자변형 이벤트에서 단편화 및 재배열을 감소시킨다.
pDAB9151 의 작제
pDAB9151 플라스미드(도 43; 서열번호 63)은 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB9151은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다. 최종 PUFA 신타제 PTU는 PvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB9148, pDAB7335, pDAB9149, pDAB9150 및 pDAB7333을 재조합하여 pDAB9151을 형성했다. 구체적으로, 상기한 4개 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 hSzThPUFA OrfC v3, SzPUFA OrfB v3, NoHetI v3, SzPUFA OrfA v3이다. pDAB7333는 또한, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
작제물 다양성을 도입하기 위한 이원 작제물 PTU 의 전사 방향의 변화
대체 작제물 설계는 PUFA 신타제 PTU 순서 및 유전자 발현 카세트의 전사 방향의 변화를 포함한다. 완성된 작제물의 제1 계열에서, 각 유전자 발현 카세트는 동일한 방향으로 위치시켰다("머리 대 꼬리", 여기서 하나의 유전자 발현 카세트의 프로모터는 제2 유전자 발현 카세트의 3' UTR에 인접하여 배치된다). 하기 작제물은 유전자 발현 카세트가 상이한 방향으로 위치되고 대체 프로모터를 사용하는 전략을 기재한다. 이들 실시예에서, 유전자 발현 카세트는 제2 유전자 발현 카세트에 대해 트랜스 방향으로 배치되어, 유전자 발현 카세트 둘 다의 프로모터가 서로에 대해 인접하여 유전자 조작된다. 이러한 배열은 "머리-대-머리" 배열로 기재된다. 다른 배열은, 하나의 유전자 발현 카세트가 제2 유전자 발현 카세트에 대해 트랜스 방향으로 배치되어, 유전자 발현 카세트 둘 다의 3' UTR이 서로에 대해 인접하여 유전자 조작되는 예에서 기재된다. 이러한 배열은 "꼬리-대-꼬리" 배열로서 기재된다. 이러한 설계의 강력한 전체 판독을 완화시키기 위해, 이방향 Orf 23/24 터미네이터를 이들 2개의 PTU 사이에 위치시켰다. 이들 배열은 변형유전자의 발현을 증가시키고, 이에 의해 고농도 및 함량의 LC-PUFA 및 DHA 지방산을 생성하는 것으로 제안된다.
pDAB108207 의 작제
pDAB108207 플라스미드(도 44; 서열번호 64)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108207은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v6, PvPhas 5' UTR, NoHetI v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다. 제2 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3, At2S SSP 터미네이터 v1 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 pvPhas 프로모터 v6, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR 및 PvPhas 3' MAR v2 (플라스미드 맵 상에 주석 없음) 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB7334, pDAB101489, pDAB108205, pDAB108206 및 pDAB7333을 재조합하여 pDAB108207을 형성했다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 꼬리-대-꼬리 배향으로 위치시키고; NoHetI v3 및 hSzThPUFA OrfC v3은 머리-대-머리 배향으로 위치시키고; hSzThPUFA OrfC v3 및 SzPUFA OrfB는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 꼬리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3, hSzThPUFA OrfC v3, SzPUFA OrfB v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB108208 의 작제
pDAB108208 플라스미드(도 45; 서열번호 65)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108208은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 PvPhas 프로모터 v4, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 pvPhas 프로모터 v5, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR, PvPhas 3' MAR v2 (플라스미드 맵 상에 주석 없음), 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB108200, pDAB101490, pDAB108201, pDAB108202 및 pDAB7333을 재조합하여 pDAB108208을 형성했다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 머리-대-머리 배향으로 위치시키고; NoHetI v3 및 hSzThPUFA OrfC v3은 꼬리-대-꼬리 배향으로 위치시키고; hSzThPUFA OrfC v3 및 SzPUFA OrfB는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 꼬리-대-꼬리 배향으로 위치시킨다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3, hSzThPUFA OrfC v3, SzPUFA OrfB v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB108209 의 작제
pDAB108209 플라스미드(도 46; 서열번호 66)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108209는 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v4, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 pvPhas 프로모터 v5, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR 및 PvPhas 3' MAR v2 (플라스미드 맵 상에 주석 없음), 및 랜덤 DNA 스페이서를 함유한다.
플라스미드 pDAB108200, pDAB108204, pDAB108201, pDAB108202 및 pDAB7333을 재조합하여 pDAB108209를 형성한다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 머리-대-머리 배향으로 위치시키고; NoHetI v3 및 hSzThPUFA OrfC v3은 꼬리-대-꼬리 배향으로 위치시키고; hSzThPUFA OrfC v3 및 SzPUFA OrfB는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 꼬리-대-꼬리 배향으로 위치시킨다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3, hSzThPUFA OrfC v3, SzPUFA OrfB v3이다. pDAB7333은, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
전사 간섭을 최소화하기 위한 3' UTR 의 배수화 및 스페이서 DNA 의 포함
전사 간섭은 다수의 유전자가 일렬로 적층되어 하류 유전자의 발현을 감소시킬 겨우에 발생할 수 있다. 이 현상은 다음 프로모터 전사 장치 속에서 3' UTR 및 터미네이터의 전사 전체 판독으로부터 일어난다. 전사 간섭을 최소화하기 위한 두 전략으로 이루어진 대체 작제 설계 및 전사 간섭이 기술된다. 제1 전략은 개별적인 DHA 유전자 발현 카세트 사이에 적층되어 다음 유전자 발현 카세트 속에서 전제 판독을 제한하는 두 개의 터미네이터/3' UTR의 사용을 배치한다. 제2 전략은 유전자 발현 카세트 사이에 스페이서 DNA의 약 1천 염기 쌍을 삽입하여 전사 간섭을 최소화한다.
pDAB108207 의 작제
pDAB108207 플라스미드(도 44; 서열번호 64)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108207은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 pvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR, PvPhas 3' MAR v2 (플라스미드 맵 상에 주석 없음), 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3, At2S SSP 터미네이터 v1 및 AtuORF23 3' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v6, PvPhas 5' UTR, NoHetI v3, PvPhas 3' UTR v1 및 PvPhas 3' MAR v2(플라스미드 맵 상에 주석 없음)를 함유한다.
플라스미드 pDAB7334, pDAB101489, pDAB108205, pDAB108206 및 pDAB7333을 재조합하여 pDAB108207을 형성했다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 꼬리-대-꼬리 배향으로 위치시키고,AtuORF23 3' UTR은 두 PTU 사이에 위치시키고; NoHetI v3 및 hSzThPUFA OrfC v3은 머리-대-머리 배향으로 위치시키고; hSzThPUFA OrfC v3 및 SzPUFA OrfB는 머리-대-꼬리 배향으로 위치시키고, AtuORF23 3' UTR은 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내의 두 PTU 사이에 위치시킨다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3, hSzThPUFA OrfC v3, SzPUFA OrfB v3이다. pDAB7333은, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB108208 의 작제
pDAB108208 플라스미드(도 45; 서열번호 65)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108208은 3개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 pvPhas 프로모터 v5, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR, PvPhas 3' MAR v2 (플라스미드 맵 상에 주석 없음) 및 AtuORF23 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v4, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB108200, pDAB101490, pDAB108201, pDAB108202 및 pDAB7333을 재조합하여 pDAB108208을 형성했다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 머리-대-머리 배향으로 위치시키고; NoHetI v3 및 hSzThPUFA OrfC v3은 꼬리-대-꼬리 배향으로 위치시키고, AtuORF23 3' UTR은 두 PTU 사이에 위치시키고; hSzThPUFA OrfC v3 및 SzPUFA OrfB는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 꼬리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3, hSzThPUFA OrfC v3, SzPUFA OrfB v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB108209 의 작제
pDAB108209 플라스미드(도 46; 서열번호 66)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB1082093개의 PUFA 신타제 PTU, 하나의 아실-CoA 신세타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 pvPhas 프로모터 v5, PvPhas 5' UTR, SzPUFA OrfB v3, PvPhas 3' UTR, PvPhas 3' MAR v2 (플라스미드 맵 상에 주석 없음), 및 랜덤 DNA 스페이서를 함유한다. 제3 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v4, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB108200, pDAB108204, pDAB108201, pDAB108202 및 pDAB7333을 재조합하여 pDAB108209를 형성했다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 머리-대-머리 배향으로 위치시키고; NoHetI v3 및 hSzThPUFA OrfC v3은 꼬리-대-꼬리 배향으로 위치시키고, 1천 염기 쌍 스페이서는 두 PTU 사이에 위치시키고; hSzThPUFA OrfC v3 및 SzPUFA OrfB는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 꼬리-대-꼬리 배향으로 위치시킨다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3, hSzThPUFA OrfC v3, SzPUFA OrfB v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
전사 전체 판독을 제한하기 위한 대체 3' UTR -터미네이터의 사용
제한된 수의 소유자 3' UTR-터미네이터에 기인하여, 아그로박테리움 ORF 23 3' UTR-터미네이터를 주로 사용하여 전사를 종결시킨다. 최근에, ZmLipase 3' UTR-터미네이터가 아라비돕시스 탈리아나에서 전사 전체 판독을 종결시키는데 더욱 효과적이다는 것이 제시되었다. 이와 같이, 작제물의 하나의 버젼은 이 3' UTR이 상류 유전자의 전사 전체 판독을 감소시켜 전사 간섭을 감소시킬 수 있는지의 여부를 시험하기 위해 ZmLipase 3' UTR-터미네이터를 pvDlec2 프로모터와 함께 사용한다.
pDAB9159 의 작제
pDAB9159 플라스미드(도 47; 서열번호 67)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB9159는 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 ZmLip 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 pvPhas 프로모터 v3, PvPhas 5' UTR, SzPUFA OrfB v3 및 ZmLip 3' UTR v1을 함유한다. 제3 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 ZmLip 3' UTR v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v3, PvPhas 5' UTR, NoHetI v3 및 ZmLip 3' UTR v1을 함유한다.
플라스미드 pDAB9152, pDAB9153, pDAB9154, pDAB9155 및 pDAB7333을 재조합하여 pDAB9159를 형성했다. 구체적으로, 상기한 4개의 PTU는 described above 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
pDAB9147 의 작제
pDAB9147 플라스미드(도 48; 서열번호 68)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB9147은 3개의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3, At2S SSP 터미네이터 v1 및 ZmLip 3' UTR v1을 함유한다. 제2 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제3 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, hSzThPUFA OrfC v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, NoHetI v3 및 At2S SSP 터미네이터 v1을 함유한다.
플라스미드 pDAB9146, pDAB7335, pDAB7336, pDAB7338 및 pDAB7333을 재조합하여 pDAB9147을 형성했다. 구체적으로, 상기한 4개의 PTU는 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-꼬리 배향으로 위치시켰다. 유전자 순서는 SzPUFA OrfA v3, SzPUFA OrfB v3, hSzThPUFA OrfC v3, NoHetI v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 5개 PTU의 도입에 대해 시험했다.
2개의 개별적 T- DNA 상에서 DHA 유전자의 전달
대체 작제 설계는 2개의 별도의 이원 벡터, 하나의 T-DNA 상에 PUFA 신타제 유전자의 서브-세트를 함유하는 제1 벡터 및 제2 T-DNA 상에 잔류하는 PUFA 신타제 유전자를 함유하는 제2 이원 벡터를 작제함으로 이루어진다. 이러한 이원 벡터를 개별적으로 사용하여 교잡된 식물을 형질전환시키고, 이에 의해 PUFA 신타제 유전자 발현 작제물 모두를 함유하는 후대를 생성한다. 유전자변형 식물을 생성하기 위한 대체 방법은 두 이원 벡터를 캐놀라 조직속에서 공동-형질전환시키고, 두 T-스트랜드를 함유하는 단일 식물을 선택하거나 선별하는 것이다.
pDAB108224 의 작제
pDAB108224 플라스미드(도 49; 서열번호 69)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108224는 하나의 PUFA 신타제 PTU, 하나의 포스포판테테이닐 트랜스퍼라제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfA v3 및 At2S SSP 터미네이터 v1을 함유한다. 포스포판테테이닐 트랜스퍼라제 PTU는 pvPhas 프로모터 v4, PvPhas 5' UTR, NoHetI v3 및 AtuORF23 3' UTR v1을 함유한다.
플라스미드 pDAB108216, pDAB108221 및 pDAB7333을 재조합하여 pDAB108224를 형성했다. 구체적으로, SzPUFA OrfA v3 및 NoHetI v3은 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-머리 배향으로 위치시킨다. 유전자 순서는 SzPUFA OrfA v3, NoHetI v3이다. pDAB7333은 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 3개 PTU의 도입에 대해 시험했다.
pDAB108225 의 작제
pDAB108225 플라스미드(도 50; 서열번호 70)를 다중 부위 입구 L-R 재조합 반응을 사용하여 작제했다. pDAB108225는 두 개의 PUFA 신타제 PTU 및 포스피노트리신 아세틸 트랜스퍼라제 PTU를 함유한다. 구체적으로, 제1 PUFA 신타제 PTU는 pvDlec2 프로모터 v2, 2S 5' UTR, SzPUFA OrfB v3 및 At2S SSP 터미네이터 v1을 함유한다. 제2 PUFA 신타제 PTU는 pvPhas 프로모터 v4, SzPUFA OrfB v3 및 Atu ORF23 3' UTR v1을 함유한다.
플라스미드 pDAB108217, pDAB108222 및 pDAB7333을 재조합하여 pDAB108225를 형성했다. 구체적으로, SzPUFA OrfB v3 및 hSzThPUFA OrfC v3을 식물 형질전환 이원 pDAB7333의 T-스트랜드 DNA 탑재 영역 내에 머리-대-머리 배향으로 위치시킨다. 유전자 순서는 SzPUFA OrfB v3, hSzThPUFA OrfC v3이다. pDAB7333은, 기타 조절 요소, 예를 들면, 과잉구동 및 T-스트랜드 탑재 서열(T-DNA 탑재 A 및 T-DNA 탑재 B) 이외에, 포스피노트리신 아세틸 트랜스퍼라제 PTU: CsVMV 프로모터 v2, PAT v5, AtuORF1 3' UTR v4를 함유한다. 이어서, 5개의 PTU를 함유하는 재조합 플라스미드를 분리하고, 제한 효소 소화 및 DNA 서열분석으로 3개 PTU의 도입에 대해 시험했다.
대체 설계를 함유하는 작제물을 사용한 캐놀라 형질전환
이들 플라스미드를 사용하여, 상기한 프로토콜을 사용하여 캐놀라 식물을 안정하게 형질전환시킨다. 유전자변형 캐놀라 식물을 분리하고, 분자적으로 특성화한다. 대체 작제물의 사용은 보다 다량의 DHA 및 LC-PUFA를 함유하는 캐놀라 식물을 생성한다. 수득된 LC-PUFA 축적을 측정하고, 0.01% 내지 15% DHA 또는 0.01% 내지 15% LC-PUFA를 함유하는 캐놀라 식물을 동정한다.
실시예 18
아라비돕시스 탈리아나의 형질전환에 사용된 대체 작제물 설계 및 LC -PUFA 및 DHA 의 후속 생산
아라비돕시스 탈리아나 식물을 pDAB101493, pDAB7362, pDAB7369, pDAB101412 또는 pDAB7380 이원 벡터를 함유하는 아그로박테리움 투멘파시엔스 균주로 형질전환시켰다. 문헌[참조: Clough and Bent (1998)]에 기재된 꽃 침지 형질전환 프로토콜을 형질전환에 사용했다[참조: Clough and Bent, "Floral dip: a simplified method for agrobacterium-mediated transformation of Arabidopsis thalia," Plant J., 16:735-743, 1998]. 형질전환된 아라비돕시스 식물을 수득하고, 변형유전자 존재의 분자 확인을 완성했다. 유전자변형 아라비돕시스 이벤트로부터 T1 식물을 온실에서 성숙기까지 성장시켰다. 이들 식물을 자가 수정하고, 생성 T2 종자를 성숙기에 수확했다. 단일 종자를 FAME GC-FID에 의해 분석하여 T2 아라비돕시스 종자에서 LC-PUFA 및 DHA 함량을 측정했다. 당해 조직은 상기 실시예에 기재된 FAME GC-FID 방법을 통해 분석했다. 아라비돕시스 식물의 T1 식물로부터의 단일 T2 종자는 0.00% 내지 0.95% DHA 및 0.00% 내지 1.50% 총 LC-PUFA를 함유했다. 개개 T1 식물로부터 각 T2 종자의 LC-PUFA 및 DHA 함량은 도 51에 제시되어 있다.
실시예 19
PUFA 신타제 유전자 세트를 사용한 "비-고 올레산" 캐놀라 품종( DH12075 )의 형질전환
브라시카 나푸스 품종 DH12075은 플라스미드 pDAB7362를 함유하는 아그로박테리움 투멘파시엔스를 사용하여 실질적으로 실시예 4에 기재된 바와 같이 배축 형질전환 방법에 의해 형질전환시켰다. 넥세라 710 유전자 배경과는 달리, DH12075는 "고올레산" 품종이 아니다. pat 유전자의 존재에 대해 포지티브인 T0 DH12075 식물을 회수하고, 실시예 5에 기재된 분자 분석 방법에 의해 DHA 유전자 세트의 5개 모두의 존재에 대해 분석했다(PUFA 신타제 OrfA, PUFA 신타제 OrfB, PUFA 신타제 키메라 OrfC, 아실-CoA 신세타제 및 4' 포스포판테테이닐 트랜스퍼라제 HetI). 이벤트 001-2009-006DH(이벤트 006)은 5개 모두의 DHA 유전자를 함유하는 T0 식물로서 동정되었다. 이를 성장 챔버에서 성숙기까지 성장시키고, T1 종자를 수확했다. 실시예 6에 기재된 방법에 의해 이벤트 006의 단일 T1 종자의 분석은 분석된 48개 종자 중의 31개가 0.19% 내지 0.86% DHA 사이의 수준으로 DHA를 함유함을 나타냈다. 113개 T1 종자를 심고, 성장 챔버에서 성장시키고, 잎 조직 시료를 실시예 4에 기재된 방법으로 분석하여 개개 식물의 접합자성을 측정했다. 23개 식물은 PAT 유전자에 대해 동형접합성인 것으로 qPCT 분석에 의해 측정되었고, 또한, 5개 DHA 유전자의 공분리를 나타냈고, 이는 단일 유전자좌의 존재를 나타낸다. 팻 및 OrfA 프로브를 사용한 이벤트 0006 T1 식물 조직의 서던 분석은 OrfA 유전자의 하나의 추가 카피가 존재함을 나타냈다. 동형접합성 식물을 성숙기까지 성장시키고, 종자를 수확했다. 이들 식물 각각으로부터 벌크 T2 종자 시료의 FAME 분석은 23개 동형접합성 T2 식물 중의 17개가 0.17 내지 0.72% 사이의 DHA 함량을 갖는 LC-PUFA를 생산했음을 나타냈다. 5개의 T2 종자 시료는 0.08% 내지 0.16%의 EPA를 함유했고, LP-PUFA 생산 이벤트의 총 LC-PUFA(DHA+EPA+DPA[n=6])는 0.33% 내지 1.35%였다. 표 22a는 이벤트 006의 2개 DHA 함유 벌크 T2 시료의 완전 지방산 프로파일을 나타낸다. 단일 종자 분석은 8개 동형접합성 T1 라인으로부터 48개 개개 T2 종자에 대해 수행했고, 이들 종자의 평균 DHA 함량은 표 23에 제시되어 있다. DHA 함량이 1.31% 이하인 단일 T2 종자가 검출되었다. 표 22b는 4개 DHA 함유 T2 종자의 완전 지방산 프로파일을 나타낸다. 이들 데이터는 DHA가 PUFA 신타제 유전자 세트에 의한 형질전환을 통해 올레산 함량이 72% 미만인 유전자 배경을 갖는 캐놀라에서 생산될 수 있음을 나타낸다.
Figure pct00022

Figure pct00023
본 발명의 상기 기재는 설명 및 서술을 목적으로 제공되었다. 더욱이, 상기 기재는 본 발명을 본원에 기재된 형태로 한정하는 것으로 의도되지 않는다.
본원에 기재된 모든 다양한 국면, 양태 및 선택사항은 임의의 및 모든 변형태로 조합될 수 있다.
본 명세서에서 언급된 모든 공개문헌, 특허 및 특허원은, 각각의 개개 공개문헌, 특허 또는 특허원이 구체적 및 개별적으로 본원에서 참조로서 도입되는 것과 동일한 정도로 본원에서 참조로서 도입된다.
<110> Dow AgroSciences LLC DSM IP Assets B.V. <120> Production of DHA and Other LC-PUFAs in Plants <130> IPA121130 <150> US 61/345,537 <151> 2010-05-17 <160> 78 <170> KopatentIn 2.0 <210> 1 <211> 2910 <212> PRT <213> Schizochytrium sp. ATCC_20888 <220> <223> SzPUFA OrfA v3 protein <300> <308> GenBank: AF378327 <309> 2007-10-22 <400> 1 Met Ala Ala Arg Leu Gln Glu Gln Lys Gly Gly Glu Met Asp Thr Arg 1 5 10 15 Ile Ala Ile Ile Gly Met Ser Ala Ile Leu Pro Cys Gly Thr Thr Val 20 25 30 Arg Glu Ser Trp Glu Thr Ile Arg Ala Gly Ile Asp Cys Leu Ser Asp 35 40 45 Leu Pro Glu Asp Arg Val Asp Val Thr Ala Tyr Phe Asp Pro Val Lys 50 55 60 Thr Thr Lys Asp Lys Ile Tyr Cys Lys Arg Gly Gly Phe Ile Pro Glu 65 70 75 80 Tyr Asp Phe Asp Ala Arg Glu Phe Gly Leu Asn Met Phe Gln Met Glu 85 90 95 Asp Ser Asp Ala Asn Gln Thr Ile Ser Leu Leu Lys Val Lys Glu Ala 100 105 110 Leu Gln Asp Ala Gly Ile Asp Ala Leu Gly Lys Glu Lys Lys Asn Ile 115 120 125 Gly Cys Val Leu Gly Ile Gly Gly Gly Gln Lys Ser Ser His Glu Phe 130 135 140 Tyr Ser Arg Leu Asn Tyr Val Val Val Glu Lys Val Leu Arg Lys Met 145 150 155 160 Gly Met Pro Glu Glu Asp Val Lys Val Ala Val Glu Lys Tyr Lys Ala 165 170 175 Asn Phe Pro Glu Trp Arg Leu Asp Ser Phe Pro Gly Phe Leu Gly Asn 180 185 190 Val Thr Ala Gly Arg Cys Thr Asn Thr Phe Asn Leu Asp Gly Met Asn 195 200 205 Cys Val Val Asp Ala Ala Cys Ala Ser Ser Leu Ile Ala Val Lys Val 210 215 220 Ala Ile Asp Glu Leu Leu Tyr Gly Asp Cys Asp Met Met Val Thr Gly 225 230 235 240 Ala Thr Cys Thr Asp Asn Ser Ile Gly Met Tyr Met Ala Phe Ser Lys 245 250 255 Thr Pro Val Phe Ser Thr Asp Pro Ser Val Arg Ala Tyr Asp Glu Lys 260 265 270 Thr Lys Gly Met Leu Ile Gly Glu Gly Ser Ala Met Leu Val Leu Lys 275 280 285 Arg Tyr Ala Asp Ala Val Arg Asp Gly Asp Glu Ile His Ala Val Ile 290 295 300 Arg Gly Cys Ala Ser Ser Ser Asp Gly Lys Ala Ala Gly Ile Tyr Thr 305 310 315 320 Pro Thr Ile Ser Gly Gln Glu Glu Ala Leu Arg Arg Ala Tyr Asn Arg 325 330 335 Ala Cys Val Asp Pro Ala Thr Val Thr Leu Val Glu Gly His Gly Thr 340 345 350 Gly Thr Pro Val Gly Asp Arg Ile Glu Leu Thr Ala Leu Arg Asn Leu 355 360 365 Phe Asp Lys Ala Tyr Gly Glu Gly Asn Thr Glu Lys Val Ala Val Gly 370 375 380 Ser Ile Lys Ser Ser Ile Gly His Leu Lys Ala Val Ala Gly Leu Ala 385 390 395 400 Gly Met Ile Lys Val Ile Met Ala Leu Lys His Lys Thr Leu Pro Gly 405 410 415 Thr Ile Asn Val Asp Asn Pro Pro Asn Leu Tyr Asp Asn Thr Pro Ile 420 425 430 Asn Glu Ser Ser Leu Tyr Ile Asn Thr Met Asn Arg Pro Trp Phe Pro 435 440 445 Pro Pro Gly Val Pro Arg Arg Ala Gly Ile Ser Ser Phe Gly Phe Gly 450 455 460 Gly Ala Asn Tyr His Ala Val Leu Glu Glu Ala Glu Pro Glu His Thr 465 470 475 480 Thr Ala Tyr Arg Leu Asn Lys Arg Pro Gln Pro Val Leu Met Met Ala 485 490 495 Ala Thr Pro Ala Ala Leu Gln Ser Leu Cys Glu Ala Gln Leu Lys Glu 500 505 510 Phe Glu Ala Ala Ile Lys Glu Asn Glu Thr Val Lys Asn Thr Ala Tyr 515 520 525 Ile Lys Cys Val Lys Phe Gly Glu Gln Phe Lys Phe Pro Gly Ser Ile 530 535 540 Pro Ala Thr Asn Ala Arg Leu Gly Phe Leu Val Lys Asp Ala Glu Asp 545 550 555 560 Ala Cys Ser Thr Leu Arg Ala Ile Cys Ala Gln Phe Ala Lys Asp Val 565 570 575 Thr Lys Glu Ala Trp Arg Leu Pro Arg Glu Gly Val Ser Phe Arg Ala 580 585 590 Lys Gly Ile Ala Thr Asn Gly Ala Val Ala Ala Leu Phe Ser Gly Gln 595 600 605 Gly Ala Gln Tyr Thr His Met Phe Ser Glu Val Ala Met Asn Trp Pro 610 615 620 Gln Phe Arg Gln Ser Ile Ala Ala Met Asp Ala Ala Gln Ser Lys Val 625 630 635 640 Ala Gly Ser Asp Lys Asp Phe Glu Arg Val Ser Gln Val Leu Tyr Pro 645 650 655 Arg Lys Pro Tyr Glu Arg Glu Pro Glu Gln Asp His Lys Lys Ile Ser 660 665 670 Leu Thr Ala Tyr Ser Gln Pro Ser Thr Leu Ala Cys Ala Leu Gly Ala 675 680 685 Phe Glu Ile Phe Lys Glu Ala Gly Phe Thr Pro Asp Phe Ala Ala Gly 690 695 700 His Ser Leu Gly Glu Phe Ala Ala Leu Tyr Ala Ala Gly Cys Val Asp 705 710 715 720 Arg Asp Glu Leu Phe Glu Leu Val Cys Arg Arg Ala Arg Ile Met Gly 725 730 735 Gly Lys Asp Ala Pro Ala Thr Pro Lys Gly Cys Met Ala Ala Val Ile 740 745 750 Gly Pro Asn Ala Glu Asn Ile Lys Val Gln Ala Ala Asn Val Trp Leu 755 760 765 Gly Asn Ser Asn Ser Pro Ser Gln Thr Val Ile Thr Gly Ser Val Glu 770 775 780 Gly Ile Gln Ala Glu Ser Ala Arg Leu Gln Lys Glu Gly Phe Arg Val 785 790 795 800 Val Pro Leu Ala Cys Glu Ser Ala Phe His Ser Pro Gln Met Glu Asn 805 810 815 Ala Ser Ser Ala Phe Lys Asp Val Ile Ser Lys Val Ser Phe Arg Thr 820 825 830 Pro Lys Ala Glu Thr Lys Leu Phe Ser Asn Val Ser Gly Glu Thr Tyr 835 840 845 Pro Thr Asp Ala Arg Glu Met Leu Thr Gln His Met Thr Ser Ser Val 850 855 860 Lys Phe Leu Thr Gln Val Arg Asn Met His Gln Ala Gly Ala Arg Ile 865 870 875 880 Phe Val Glu Phe Gly Pro Lys Gln Val Leu Ser Lys Leu Val Ser Glu 885 890 895 Thr Leu Lys Asp Asp Pro Ser Val Val Thr Val Ser Val Asn Pro Ala 900 905 910 Ser Gly Thr Asp Ser Asp Ile Gln Leu Arg Asp Ala Ala Val Gln Leu 915 920 925 Val Val Ala Gly Val Asn Leu Gln Gly Phe Asp Lys Trp Asp Ala Pro 930 935 940 Asp Ala Thr Arg Met Gln Ala Ile Lys Lys Lys Arg Thr Thr Leu Arg 945 950 955 960 Leu Ser Ala Ala Thr Tyr Val Ser Asp Lys Thr Lys Lys Val Arg Asp 965 970 975 Ala Ala Met Asn Asp Gly Arg Cys Val Thr Tyr Leu Lys Gly Ala Ala 980 985 990 Pro Leu Ile Lys Ala Pro Glu Pro Val Val Asp Glu Ala Ala Lys Arg 995 1000 1005 Glu Ala Glu Arg Leu Gln Lys Glu Leu Gln Asp Ala Gln Arg Gln 1010 1015 1020 Leu Asp Asp Ala Lys Arg Ala Ala Ala Glu Ala Asn Ser Lys Leu 1025 1030 1035 Ala Ala Ala Lys Glu Glu Ala Lys Thr Ala Ala Ala Ser Ala Lys 1040 1045 1050 Pro Ala Val Asp Thr Ala Val Val Glu Lys His Arg Ala Ile Leu 1055 1060 1065 Lys Ser Met Leu Ala Glu Leu Asp Gly Tyr Gly Ser Val Asp Ala 1070 1075 1080 Ser Ser Leu Gln Gln Gln Gln Gln Gln Gln Thr Ala Pro Ala Pro 1085 1090 1095 Val Lys Ala Ala Ala Pro Ala Ala Pro Val Ala Ser Ala Pro Ala 1100 1105 1110 Pro Ala Val Ser Asn Glu Leu Leu Glu Lys Ala Glu Thr Val Val 1115 1120 1125 Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp Met Ile 1130 1135 1140 Glu Ala Asp Met Glu Leu Glu Thr Glu Leu Gly Ile Asp Ser Ile 1145 1150 1155 Lys Arg Val Glu Ile Leu Ser Glu Val Gln Ala Met Leu Asn Val 1160 1165 1170 Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr Arg Thr Val Gly 1175 1180 1185 Glu Val Val Asn Ala Met Lys Ala Glu Ile Ala Gly Ser Ser Ala 1190 1195 1200 Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Lys Ala Ala Pro 1205 1210 1215 Ala Ala Ala Ala Pro Ala Val Ser Asn Glu Leu Leu Glu Lys Ala 1220 1225 1230 Glu Thr Val Val Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu 1235 1240 1245 Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr Glu Leu Gly 1250 1255 1260 Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Ser Glu Val Gln Ala 1265 1270 1275 Met Leu Asn Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr 1280 1285 1290 Arg Thr Val Gly Glu Val Val Asn Ala Met Lys Ala Glu Ile Ala 1295 1300 1305 Gly Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro Gly Pro Ala 1310 1315 1320 Ala Ala Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Val Ser Asn 1325 1330 1335 Glu Leu Leu Glu Lys Ala Glu Thr Val Val Met Glu Val Leu Ala 1340 1345 1350 Ala Lys Thr Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu 1355 1360 1365 Leu Glu Thr Glu Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile 1370 1375 1380 Leu Ser Glu Val Gln Ala Met Leu Asn Val Glu Ala Lys Asp Val 1385 1390 1395 Asp Ala Leu Ser Arg Thr Arg Thr Val Gly Glu Val Val Asp Ala 1400 1405 1410 Met Lys Ala Glu Ile Ala Gly Gly Ser Ala Pro Ala Pro Ala Ala 1415 1420 1425 Ala Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala Pro 1430 1435 1440 Ala Pro Ala Val Ser Ser Glu Leu Leu Glu Lys Ala Glu Thr Val 1445 1450 1455 Val Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp Met 1460 1465 1470 Ile Glu Ser Asp Met Glu Leu Glu Thr Glu Leu Gly Ile Asp Ser 1475 1480 1485 Ile Lys Arg Val Glu Ile Leu Ser Glu Val Gln Ala Met Leu Asn 1490 1495 1500 Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr Arg Thr Val 1505 1510 1515 Gly Glu Val Val Asp Ala Met Lys Ala Glu Ile Ala Gly Gly Ser 1520 1525 1530 Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala Ala 1535 1540 1545 Pro Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Pro Ala Val 1550 1555 1560 Ser Ser Glu Leu Leu Glu Lys Ala Glu Thr Val Val Met Glu Val 1565 1570 1575 Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp 1580 1585 1590 Met Glu Leu Glu Thr Glu Leu Gly Ile Asp Ser Ile Lys Arg Val 1595 1600 1605 Glu Ile Leu Ser Glu Val Gln Ala Met Leu Asn Val Glu Ala Lys 1610 1615 1620 Asp Val Asp Ala Leu Ser Arg Thr Arg Thr Val Gly Glu Val Val 1625 1630 1635 Asp Ala Met Lys Ala Glu Ile Ala Gly Ser Ser Ala Ser Ala Pro 1640 1645 1650 Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala 1655 1660 1665 Ala Ala Ala Pro Ala Val Ser Asn Glu Leu Leu Glu Lys Ala Glu 1670 1675 1680 Thr Val Val Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr 1685 1690 1695 Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr Glu Leu Gly Ile 1700 1705 1710 Asp Ser Ile Lys Arg Val Glu Ile Leu Ser Glu Val Gln Ala Met 1715 1720 1725 Leu Asn Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr Arg 1730 1735 1740 Thr Val Gly Glu Val Val Asp Ala Met Lys Ala Glu Ile Ala Gly 1745 1750 1755 Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala 1760 1765 1770 Ala Ala Pro Ala Val Ser Asn Glu Leu Leu Glu Lys Ala Glu Thr 1775 1780 1785 Val Val Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp 1790 1795 1800 Met Ile Glu Ser Asp Met Glu Leu Glu Thr Glu Leu Gly Ile Asp 1805 1810 1815 Ser Ile Lys Arg Val Glu Ile Leu Ser Glu Val Gln Ala Met Leu 1820 1825 1830 Asn Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr Arg Thr 1835 1840 1845 Val Gly Glu Val Val Asp Ala Met Lys Ala Glu Ile Ala Gly Ser 1850 1855 1860 Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala 1865 1870 1875 Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Val Ser Ser Glu Leu 1880 1885 1890 Leu Glu Lys Ala Glu Thr Val Val Met Glu Val Leu Ala Ala Lys 1895 1900 1905 Thr Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu 1910 1915 1920 Thr Glu Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Ser 1925 1930 1935 Glu Val Gln Ala Met Leu Asn Val Glu Ala Lys Asp Val Asp Ala 1940 1945 1950 Leu Ser Arg Thr Arg Thr Val Gly Glu Val Val Asp Ala Met Lys 1955 1960 1965 Ala Glu Ile Ala Gly Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala 1970 1975 1980 Pro Ala Pro Ala Ala Ala Ala Pro Ala Val Ser Asn Glu Leu Leu 1985 1990 1995 Glu Lys Ala Glu Thr Val Val Met Glu Val Leu Ala Ala Lys Thr 2000 2005 2010 Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr 2015 2020 2025 Glu Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Ser Glu 2030 2035 2040 Val Gln Ala Met Leu Asn Val Glu Ala Lys Asp Val Asp Ala Leu 2045 2050 2055 Ser Arg Thr Arg Thr Val Gly Glu Val Val Asp Ala Met Lys Ala 2060 2065 2070 Glu Ile Ala Gly Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro 2075 2080 2085 Ala Ser Ala Gly Ala Ala Pro Ala Val Lys Ile Asp Ser Val His 2090 2095 2100 Gly Ala Asp Cys Asp Asp Leu Ser Leu Met His Ala Lys Val Val 2105 2110 2115 Asp Ile Arg Arg Pro Asp Glu Leu Ile Leu Glu Arg Pro Glu Asn 2120 2125 2130 Arg Pro Val Leu Val Val Asp Asp Gly Ser Glu Leu Thr Leu Ala 2135 2140 2145 Leu Val Arg Val Leu Gly Ala Cys Ala Val Val Leu Thr Phe Glu 2150 2155 2160 Gly Leu Gln Leu Ala Gln Arg Ala Gly Ala Ala Ala Ile Arg His 2165 2170 2175 Val Leu Ala Lys Asp Leu Ser Ala Glu Ser Ala Glu Lys Ala Ile 2180 2185 2190 Lys Glu Ala Glu Gln Arg Phe Gly Ala Leu Gly Gly Phe Ile Ser 2195 2200 2205 Gln Gln Ala Glu Arg Phe Glu Pro Ala Glu Ile Leu Gly Phe Thr 2210 2215 2220 Leu Met Cys Ala Lys Phe Ala Lys Ala Ser Leu Cys Thr Ala Val 2225 2230 2235 Ala Gly Gly Arg Pro Ala Phe Ile Gly Val Ala Arg Leu Asp Gly 2240 2245 2250 Arg Leu Gly Phe Thr Ser Gln Gly Thr Ser Asp Ala Leu Lys Arg 2255 2260 2265 Ala Gln Arg Gly Ala Ile Phe Gly Leu Cys Lys Thr Ile Gly Leu 2270 2275 2280 Glu Trp Ser Glu Ser Asp Val Phe Ser Arg Gly Val Asp Ile Ala 2285 2290 2295 Gln Gly Met His Pro Glu Asp Ala Ala Val Ala Ile Val Arg Glu 2300 2305 2310 Met Ala Cys Ala Asp Ile Arg Ile Arg Glu Val Gly Ile Gly Ala 2315 2320 2325 Asn Gln Gln Arg Cys Thr Ile Arg Ala Ala Lys Leu Glu Thr Gly 2330 2335 2340 Asn Pro Gln Arg Gln Ile Ala Lys Asp Asp Val Leu Leu Val Ser 2345 2350 2355 Gly Gly Ala Arg Gly Ile Thr Pro Leu Cys Ile Arg Glu Ile Thr 2360 2365 2370 Arg Gln Ile Ala Gly Gly Lys Tyr Ile Leu Leu Gly Arg Ser Lys 2375 2380 2385 Val Ser Ala Ser Glu Pro Ala Trp Cys Ala Gly Ile Thr Asp Glu 2390 2395 2400 Lys Ala Val Gln Lys Ala Ala Thr Gln Glu Leu Lys Arg Ala Phe 2405 2410 2415 Ser Ala Gly Glu Gly Pro Lys Pro Thr Pro Arg Ala Val Thr Lys 2420 2425 2430 Leu Val Gly Ser Val Leu Gly Ala Arg Glu Val Arg Ser Ser Ile 2435 2440 2445 Ala Ala Ile Glu Ala Leu Gly Gly Lys Ala Ile Tyr Ser Ser Cys 2450 2455 2460 Asp Val Asn Ser Ala Ala Asp Val Ala Lys Ala Val Arg Asp Ala 2465 2470 2475 Glu Ser Gln Leu Gly Ala Arg Val Ser Gly Ile Val His Ala Ser 2480 2485 2490 Gly Val Leu Arg Asp Arg Leu Ile Glu Lys Lys Leu Pro Asp Glu 2495 2500 2505 Phe Asp Ala Val Phe Gly Thr Lys Val Thr Gly Leu Glu Asn Leu 2510 2515 2520 Leu Ala Ala Val Asp Arg Ala Asn Leu Lys His Met Val Leu Phe 2525 2530 2535 Ser Ser Leu Ala Gly Phe His Gly Asn Val Gly Gln Ser Asp Tyr 2540 2545 2550 Ala Met Ala Asn Glu Ala Leu Asn Lys Met Gly Leu Glu Leu Ala 2555 2560 2565 Lys Asp Val Ser Val Lys Ser Ile Cys Phe Gly Pro Trp Asp Gly 2570 2575 2580 Gly Met Val Thr Pro Gln Leu Lys Lys Gln Phe Gln Glu Met Gly 2585 2590 2595 Val Gln Ile Ile Pro Arg Glu Gly Gly Ala Asp Thr Val Ala Arg 2600 2605 2610 Ile Val Leu Gly Ser Ser Pro Ala Glu Ile Leu Val Gly Asn Trp 2615 2620 2625 Arg Thr Pro Ser Lys Lys Val Gly Ser Asp Thr Ile Thr Leu His 2630 2635 2640 Arg Lys Ile Ser Ala Lys Ser Asn Pro Phe Leu Glu Asp His Val 2645 2650 2655 Ile Gln Gly Arg Arg Val Leu Pro Met Thr Leu Ala Ile Gly Ser 2660 2665 2670 Leu Ala Glu Thr Cys Leu Gly Leu Phe Pro Gly Tyr Ser Leu Trp 2675 2680 2685 Ala Ile Asp Asp Ala Gln Leu Phe Lys Gly Val Thr Val Asp Gly 2690 2695 2700 Asp Val Asn Cys Glu Val Thr Leu Thr Pro Ser Thr Ala Pro Ser 2705 2710 2715 Gly Arg Val Asn Val Gln Ala Thr Leu Lys Thr Phe Ser Ser Gly 2720 2725 2730 Lys Leu Val Pro Ala Tyr Arg Ala Val Ile Val Leu Ser Asn Gln 2735 2740 2745 Gly Ala Pro Pro Ala Asn Ala Thr Met Gln Pro Pro Ser Leu Asp 2750 2755 2760 Ala Asp Pro Ala Leu Gln Gly Ser Val Tyr Asp Gly Lys Thr Leu 2765 2770 2775 Phe His Gly Pro Ala Phe Arg Gly Ile Asp Asp Val Leu Ser Cys 2780 2785 2790 Thr Lys Ser Gln Leu Val Ala Lys Cys Ser Ala Val Pro Gly Ser 2795 2800 2805 Asp Ala Ala Arg Gly Glu Phe Ala Thr Asp Thr Asp Ala His Asp 2810 2815 2820 Pro Phe Val Asn Asp Leu Ala Phe Gln Ala Met Leu Val Trp Val 2825 2830 2835 Arg Arg Thr Leu Gly Gln Ala Ala Leu Pro Asn Ser Ile Gln Arg 2840 2845 2850 Ile Val Gln His Arg Pro Val Pro Gln Asp Lys Pro Phe Tyr Ile 2855 2860 2865 Thr Leu Arg Ser Asn Gln Ser Gly Gly His Ser Gln His Lys His 2870 2875 2880 Ala Leu Gln Phe His Asn Glu Gln Gly Asp Leu Phe Ile Asp Val 2885 2890 2895 Gln Ala Ser Val Ile Ala Thr Asp Ser Leu Ala Phe 2900 2905 2910 <210> 2 <211> 2059 <212> PRT <213> Schizochytrium sp. ATCC_20888 <220> <223> SzPUFA OrfB v3 protein <300> <308> GenBank: AF378328 <309> 2007-10-22 <400> 2 Met Ala Ala Arg Asn Val Ser Ala Ala His Glu Met His Asp Glu Lys 1 5 10 15 Arg Ile Ala Val Val Gly Met Ala Val Gln Tyr Ala Gly Cys Lys Thr 20 25 30 Lys Asp Glu Phe Trp Glu Val Leu Met Asn Gly Lys Val Glu Ser Lys 35 40 45 Val Ile Ser Asp Lys Arg Leu Gly Ser Asn Tyr Arg Ala Glu His Tyr 50 55 60 Lys Ala Glu Arg Ser Lys Tyr Ala Asp Thr Phe Cys Asn Glu Thr Tyr 65 70 75 80 Gly Thr Leu Asp Glu Asn Glu Ile Asp Asn Glu His Glu Leu Leu Leu 85 90 95 Asn Leu Ala Lys Gln Ala Leu Ala Glu Thr Ser Val Lys Asp Ser Thr 100 105 110 Arg Cys Gly Ile Val Ser Gly Cys Leu Ser Phe Pro Met Asp Asn Leu 115 120 125 Gln Gly Glu Leu Leu Asn Val Tyr Gln Asn His Val Glu Lys Lys Leu 130 135 140 Gly Ala Arg Val Phe Lys Asp Ala Ser His Trp Ser Glu Arg Glu Gln 145 150 155 160 Ser Asn Lys Pro Glu Ala Gly Asp Arg Arg Ile Phe Met Asp Pro Ala 165 170 175 Ser Phe Val Ala Glu Glu Leu Asn Leu Gly Ala Leu His Tyr Ser Val 180 185 190 Asp Ala Ala Cys Ala Thr Ala Leu Tyr Val Leu Arg Leu Ala Gln Asp 195 200 205 His Leu Val Ser Gly Ala Ala Asp Val Met Leu Cys Gly Ala Thr Cys 210 215 220 Leu Pro Glu Pro Phe Phe Ile Leu Ser Gly Phe Ser Thr Phe Gln Ala 225 230 235 240 Met Pro Val Gly Thr Gly Gln Asn Val Ser Met Pro Leu His Lys Asp 245 250 255 Ser Gln Gly Leu Thr Pro Gly Glu Gly Gly Ser Ile Met Val Leu Lys 260 265 270 Arg Leu Asp Asp Ala Ile Arg Asp Gly Asp His Ile Tyr Gly Thr Leu 275 280 285 Leu Gly Ala Asn Val Ser Asn Ser Gly Thr Gly Leu Pro Leu Lys Pro 290 295 300 Leu Leu Pro Ser Glu Lys Lys Cys Leu Met Asp Thr Tyr Thr Arg Ile 305 310 315 320 Asn Val His Pro His Lys Ile Gln Tyr Val Glu Cys His Ala Thr Gly 325 330 335 Thr Pro Gln Gly Asp Arg Val Glu Ile Asp Ala Val Lys Ala Cys Phe 340 345 350 Glu Gly Lys Val Pro Arg Phe Gly Thr Thr Lys Gly Asn Phe Gly His 355 360 365 Thr Leu Val Ala Ala Gly Phe Ala Gly Met Cys Lys Val Leu Leu Ser 370 375 380 Met Lys His Gly Ile Ile Pro Pro Thr Pro Gly Ile Asp Asp Glu Thr 385 390 395 400 Lys Met Asp Pro Leu Val Val Ser Gly Glu Ala Ile Pro Trp Pro Glu 405 410 415 Thr Asn Gly Glu Pro Lys Arg Ala Gly Leu Ser Ala Phe Gly Phe Gly 420 425 430 Gly Thr Asn Ala His Ala Val Phe Glu Glu His Asp Pro Ser Asn Ala 435 440 445 Ala Cys Thr Gly His Asp Ser Ile Ser Ala Leu Ser Ala Arg Cys Gly 450 455 460 Gly Glu Ser Asn Met Arg Ile Ala Ile Thr Gly Met Asp Ala Thr Phe 465 470 475 480 Gly Ala Leu Lys Gly Leu Asp Ala Phe Glu Arg Ala Ile Tyr Thr Gly 485 490 495 Ala His Gly Ala Ile Pro Leu Pro Glu Lys Arg Trp Arg Phe Leu Gly 500 505 510 Lys Asp Lys Asp Phe Leu Asp Leu Cys Gly Val Lys Ala Thr Pro His 515 520 525 Gly Cys Tyr Ile Glu Asp Val Glu Val Asp Phe Gln Arg Leu Arg Thr 530 535 540 Pro Met Thr Pro Glu Asp Met Leu Leu Pro Gln Gln Leu Leu Ala Val 545 550 555 560 Thr Thr Ile Asp Arg Ala Ile Leu Asp Ser Gly Met Lys Lys Gly Gly 565 570 575 Asn Val Ala Val Phe Val Gly Leu Gly Thr Asp Leu Glu Leu Tyr Arg 580 585 590 His Arg Ala Arg Val Ala Leu Lys Glu Arg Val Arg Pro Glu Ala Ser 595 600 605 Lys Lys Leu Asn Asp Met Met Gln Tyr Ile Asn Asp Cys Gly Thr Ser 610 615 620 Thr Ser Tyr Thr Ser Tyr Ile Gly Asn Leu Val Ala Thr Arg Val Ser 625 630 635 640 Ser Gln Trp Gly Phe Thr Gly Pro Ser Phe Thr Ile Thr Glu Gly Asn 645 650 655 Asn Ser Val Tyr Arg Cys Ala Glu Leu Gly Lys Tyr Leu Leu Glu Thr 660 665 670 Gly Glu Val Asp Gly Val Val Val Ala Gly Val Asp Leu Cys Gly Ser 675 680 685 Ala Glu Asn Leu Tyr Val Lys Ser Arg Arg Phe Lys Val Ser Thr Ser 690 695 700 Asp Thr Pro Arg Ala Ser Phe Asp Ala Ala Ala Asp Gly Tyr Phe Val 705 710 715 720 Gly Glu Gly Cys Gly Ala Phe Val Leu Lys Arg Glu Thr Ser Cys Thr 725 730 735 Lys Asp Asp Arg Ile Tyr Ala Cys Met Asp Ala Ile Val Pro Gly Asn 740 745 750 Val Pro Ser Ala Cys Leu Arg Glu Ala Leu Asp Gln Ala Arg Val Lys 755 760 765 Pro Gly Asp Ile Glu Met Leu Glu Leu Ser Ala Asp Ser Ala Arg His 770 775 780 Leu Lys Asp Pro Ser Val Leu Pro Lys Glu Leu Thr Ala Glu Glu Glu 785 790 795 800 Ile Gly Gly Leu Gln Thr Ile Leu Arg Asp Asp Asp Lys Leu Pro Arg 805 810 815 Asn Val Ala Thr Gly Ser Val Lys Ala Thr Val Gly Asp Thr Gly Tyr 820 825 830 Ala Ser Gly Ala Ala Ser Leu Ile Lys Ala Ala Leu Cys Ile Tyr Asn 835 840 845 Arg Tyr Leu Pro Ser Asn Gly Asp Asp Trp Asp Glu Pro Ala Pro Glu 850 855 860 Ala Pro Trp Asp Ser Thr Leu Phe Ala Cys Gln Thr Ser Arg Ala Trp 865 870 875 880 Leu Lys Asn Pro Gly Glu Arg Arg Tyr Ala Ala Val Ser Gly Val Ser 885 890 895 Glu Thr Arg Ser Cys Tyr Ser Val Leu Leu Ser Glu Ala Glu Gly His 900 905 910 Tyr Glu Arg Glu Asn Arg Ile Ser Leu Asp Glu Glu Ala Pro Lys Leu 915 920 925 Ile Val Leu Arg Ala Asp Ser His Glu Glu Ile Leu Gly Arg Leu Asp 930 935 940 Lys Ile Arg Glu Arg Phe Leu Gln Pro Thr Gly Ala Ala Pro Arg Glu 945 950 955 960 Ser Glu Leu Lys Ala Gln Ala Arg Arg Ile Phe Leu Glu Leu Leu Gly 965 970 975 Glu Thr Leu Ala Gln Asp Ala Ala Ser Ser Gly Ser Gln Lys Pro Leu 980 985 990 Ala Leu Ser Leu Val Ser Thr Pro Ser Lys Leu Gln Arg Glu Val Glu 995 1000 1005 Leu Ala Ala Lys Gly Ile Pro Arg Cys Leu Lys Met Arg Arg Asp 1010 1015 1020 Trp Ser Ser Pro Ala Gly Ser Arg Tyr Ala Pro Glu Pro Leu Ala 1025 1030 1035 Ser Asp Arg Val Ala Phe Met Tyr Gly Glu Gly Arg Ser Pro Tyr 1040 1045 1050 Tyr Gly Ile Thr Gln Asp Ile His Arg Ile Trp Pro Glu Leu His 1055 1060 1065 Glu Val Ile Asn Glu Lys Thr Asn Arg Leu Trp Ala Glu Gly Asp 1070 1075 1080 Arg Trp Val Met Pro Arg Ala Ser Phe Lys Ser Glu Leu Glu Ser 1085 1090 1095 Gln Gln Gln Glu Phe Asp Arg Asn Met Ile Glu Met Phe Arg Leu 1100 1105 1110 Gly Ile Leu Thr Ser Ile Ala Phe Thr Asn Leu Ala Arg Asp Val 1115 1120 1125 Leu Asn Ile Thr Pro Lys Ala Ala Phe Gly Leu Ser Leu Gly Glu 1130 1135 1140 Ile Ser Met Ile Phe Ala Phe Ser Lys Lys Asn Gly Leu Ile Ser 1145 1150 1155 Asp Gln Leu Thr Lys Asp Leu Arg Glu Ser Asp Val Trp Asn Lys 1160 1165 1170 Ala Leu Ala Val Glu Phe Asn Ala Leu Arg Glu Ala Trp Gly Ile 1175 1180 1185 Pro Gln Ser Val Pro Lys Asp Glu Phe Trp Gln Gly Tyr Ile Val 1190 1195 1200 Arg Gly Thr Lys Gln Asp Ile Glu Ala Ala Ile Ala Pro Asp Ser 1205 1210 1215 Lys Tyr Val Arg Leu Thr Ile Ile Asn Asp Ala Asn Thr Ala Leu 1220 1225 1230 Ile Ser Gly Lys Pro Asp Ala Cys Lys Ala Ala Ile Ala Arg Leu 1235 1240 1245 Gly Gly Asn Ile Pro Ala Leu Pro Val Thr Gln Gly Met Cys Gly 1250 1255 1260 His Cys Pro Glu Val Gly Pro Tyr Thr Lys Asp Ile Ala Lys Ile 1265 1270 1275 His Ala Asn Leu Glu Phe Pro Val Val Asp Gly Leu Asp Leu Trp 1280 1285 1290 Thr Thr Ile Asn Gln Lys Arg Leu Val Pro Arg Ala Thr Gly Ala 1295 1300 1305 Lys Asp Glu Trp Ala Pro Ser Ser Phe Gly Glu Tyr Ala Gly Gln 1310 1315 1320 Leu Tyr Glu Lys Gln Ala Asn Phe Pro Gln Ile Val Glu Thr Ile 1325 1330 1335 Tyr Lys Gln Asn Tyr Asp Val Phe Val Glu Val Gly Pro Asn Asn 1340 1345 1350 His Arg Ser Thr Ala Val Arg Thr Thr Leu Gly Pro Gln Arg Asn 1355 1360 1365 His Leu Ala Gly Ala Ile Asp Lys Gln Asn Glu Asp Ala Trp Thr 1370 1375 1380 Thr Ile Val Lys Leu Val Ala Ser Leu Lys Ala His Leu Val Pro 1385 1390 1395 Gly Val Thr Ile Ser Pro Leu Tyr His Ser Lys Leu Val Ala Glu 1400 1405 1410 Ala Glu Ala Cys Tyr Ala Ala Leu Cys Lys Gly Glu Lys Pro Lys 1415 1420 1425 Lys Asn Lys Phe Val Arg Lys Ile Gln Leu Asn Gly Arg Phe Asn 1430 1435 1440 Ser Lys Ala Asp Pro Ile Ser Ser Ala Asp Leu Ala Ser Phe Pro 1445 1450 1455 Pro Ala Asp Pro Ala Ile Glu Ala Ala Ile Ser Ser Arg Ile Met 1460 1465 1470 Lys Pro Val Ala Pro Lys Phe Tyr Ala Arg Leu Asn Ile Asp Glu 1475 1480 1485 Gln Asp Glu Thr Arg Asp Pro Ile Leu Asn Lys Asp Asn Ala Pro 1490 1495 1500 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 1505 1510 1515 Pro Ser Pro Ala Pro Ser Ala Pro Val Gln Lys Lys Ala Ala Pro 1520 1525 1530 Ala Ala Glu Thr Lys Ala Val Ala Ser Ala Asp Ala Leu Arg Ser 1535 1540 1545 Ala Leu Leu Asp Leu Asp Ser Met Leu Ala Leu Ser Ser Ala Ser 1550 1555 1560 Ala Ser Gly Asn Leu Val Glu Thr Ala Pro Ser Asp Ala Ser Val 1565 1570 1575 Ile Val Pro Pro Cys Asn Ile Ala Asp Leu Gly Ser Arg Ala Phe 1580 1585 1590 Met Lys Thr Tyr Gly Val Ser Ala Pro Leu Tyr Thr Gly Ala Met 1595 1600 1605 Ala Lys Gly Ile Ala Ser Ala Asp Leu Val Ile Ala Ala Gly Arg 1610 1615 1620 Gln Gly Ile Leu Ala Ser Phe Gly Ala Gly Gly Leu Pro Met Gln 1625 1630 1635 Val Val Arg Glu Ser Ile Glu Lys Ile Gln Ala Ala Leu Pro Asn 1640 1645 1650 Gly Pro Tyr Ala Val Asn Leu Ile His Ser Pro Phe Asp Ser Asn 1655 1660 1665 Leu Glu Lys Gly Asn Val Asp Leu Phe Leu Glu Lys Gly Val Thr 1670 1675 1680 Phe Val Glu Ala Ser Ala Phe Met Thr Leu Thr Pro Gln Val Val 1685 1690 1695 Arg Tyr Arg Ala Ala Gly Leu Thr Arg Asn Ala Asp Gly Ser Val 1700 1705 1710 Asn Ile Arg Asn Arg Ile Ile Gly Lys Val Ser Arg Thr Glu Leu 1715 1720 1725 Ala Glu Met Phe Met Arg Pro Ala Pro Glu His Leu Leu Gln Lys 1730 1735 1740 Leu Ile Ala Ser Gly Glu Ile Asn Gln Glu Gln Ala Glu Leu Ala 1745 1750 1755 Arg Arg Val Pro Val Ala Asp Asp Ile Ala Val Glu Ala Asp Ser 1760 1765 1770 Gly Gly His Thr Asp Asn Arg Pro Ile His Val Ile Leu Pro Leu 1775 1780 1785 Ile Ile Asn Leu Arg Asp Arg Leu His Arg Glu Cys Gly Tyr Pro 1790 1795 1800 Ala Asn Leu Arg Val Arg Val Gly Ala Gly Gly Gly Ile Gly Cys 1805 1810 1815 Pro Gln Ala Ala Leu Ala Thr Phe Asn Met Gly Ala Ser Phe Ile 1820 1825 1830 Val Thr Gly Thr Val Asn Gln Val Ala Lys Gln Ser Gly Thr Cys 1835 1840 1845 Asp Asn Val Arg Lys Gln Leu Ala Lys Ala Thr Tyr Ser Asp Val 1850 1855 1860 Cys Met Ala Pro Ala Ala Asp Met Phe Glu Glu Gly Val Lys Leu 1865 1870 1875 Gln Val Leu Lys Lys Gly Thr Met Phe Pro Ser Arg Ala Asn Lys 1880 1885 1890 Leu Tyr Glu Leu Phe Cys Lys Tyr Asp Ser Phe Glu Ser Met Pro 1895 1900 1905 Pro Ala Glu Leu Ala Arg Val Glu Lys Arg Ile Phe Ser Arg Ala 1910 1915 1920 Leu Glu Glu Val Trp Asp Glu Thr Lys Asn Phe Tyr Ile Asn Arg 1925 1930 1935 Leu His Asn Pro Glu Lys Ile Gln Arg Ala Glu Arg Asp Pro Lys 1940 1945 1950 Leu Lys Met Ser Leu Cys Phe Arg Trp Tyr Leu Ser Leu Ala Ser 1955 1960 1965 Arg Trp Ala Asn Thr Gly Ala Ser Asp Arg Val Met Asp Tyr Gln 1970 1975 1980 Val Trp Cys Gly Pro Ala Ile Gly Ser Phe Asn Asp Phe Ile Lys 1985 1990 1995 Gly Thr Tyr Leu Asp Pro Ala Val Ala Asn Glu Tyr Pro Cys Val 2000 2005 2010 Val Gln Ile Asn Lys Gln Ile Leu Arg Gly Ala Cys Phe Leu Arg 2015 2020 2025 Arg Leu Glu Ile Leu Arg Asn Ala Arg Leu Ser Asp Gly Ala Ala 2030 2035 2040 Ala Leu Val Ala Ser Ile Asp Asp Thr Tyr Val Pro Ala Glu Lys 2045 2050 2055 Leu <210> 3 <211> 1493 <212> PRT <213> Artificial sequence <220> <223> Chimeric PUFA OrfC from Schizochytrium sp. ATCC_20888 and Thraustochytrium <220> <223> hSzThPUFA OrfC v3 protein <300> <302> CHIMERIC PUFA POLYKETIDE SYNTHASE SYSTEMS AND USES THEREOF <310> US 2008-0022422 A1 <311> 2007-05-16 <312> 2008-01-24 <400> 3 Met Ala Leu Arg Val Lys Thr Asn Lys Lys Pro Cys Trp Glu Met Thr 1 5 10 15 Lys Glu Glu Leu Thr Ser Gly Lys Thr Glu Val Phe Asn Tyr Glu Glu 20 25 30 Leu Leu Glu Phe Ala Glu Gly Asp Ile Ala Lys Val Phe Gly Pro Glu 35 40 45 Phe Ala Val Ile Asp Lys Tyr Pro Arg Arg Val Arg Leu Pro Ala Arg 50 55 60 Glu Tyr Leu Leu Val Thr Arg Val Thr Leu Met Asp Ala Glu Val Asn 65 70 75 80 Asn Tyr Arg Val Gly Ala Arg Met Val Thr Glu Tyr Asp Leu Pro Val 85 90 95 Asn Gly Glu Leu Ser Glu Gly Gly Asp Cys Pro Trp Ala Val Leu Val 100 105 110 Glu Ser Gly Gln Cys Asp Leu Met Leu Ile Ser Tyr Met Gly Ile Asp 115 120 125 Phe Gln Asn Gln Gly Asp Arg Val Tyr Arg Leu Leu Asn Thr Thr Leu 130 135 140 Thr Phe Tyr Gly Val Ala His Glu Gly Glu Thr Leu Glu Tyr Asp Ile 145 150 155 160 Arg Val Thr Gly Phe Ala Lys Arg Leu Asp Gly Gly Ile Ser Met Phe 165 170 175 Phe Phe Glu Tyr Asp Cys Tyr Val Asn Gly Arg Leu Leu Ile Glu Met 180 185 190 Arg Asp Gly Cys Ala Gly Phe Phe Thr Asn Glu Glu Leu Asp Ala Gly 195 200 205 Lys Gly Val Val Phe Thr Arg Gly Asp Leu Ala Ala Arg Ala Lys Ile 210 215 220 Pro Lys Gln Asp Val Ser Pro Tyr Ala Val Ala Pro Cys Leu His Lys 225 230 235 240 Thr Lys Leu Asn Glu Lys Glu Met Gln Thr Leu Val Asp Lys Asp Trp 245 250 255 Ala Ser Val Phe Gly Ser Lys Asn Gly Met Pro Glu Ile Asn Tyr Lys 260 265 270 Leu Cys Ala Arg Lys Met Leu Met Ile Asp Arg Val Thr Ser Ile Asp 275 280 285 His Lys Gly Gly Val Tyr Gly Leu Gly Gln Leu Val Gly Glu Lys Ile 290 295 300 Leu Glu Arg Asp His Trp Tyr Phe Pro Cys His Phe Val Lys Asp Gln 305 310 315 320 Val Met Ala Gly Ser Leu Val Ser Asp Gly Cys Ser Gln Met Leu Lys 325 330 335 Met Tyr Met Ile Trp Leu Gly Leu His Leu Thr Thr Gly Pro Phe Asp 340 345 350 Phe Arg Pro Val Asn Gly His Pro Asn Lys Val Arg Cys Arg Gly Gln 355 360 365 Ile Ser Pro His Lys Gly Lys Leu Val Tyr Val Met Glu Ile Lys Glu 370 375 380 Met Gly Phe Asp Glu Asp Asn Asp Pro Tyr Ala Ile Ala Asp Val Asn 385 390 395 400 Ile Ile Asp Val Asp Phe Glu Lys Gly Gln Asp Phe Ser Leu Asp Arg 405 410 415 Ile Ser Asp Tyr Gly Lys Gly Asp Leu Asn Lys Lys Ile Val Val Asp 420 425 430 Phe Lys Gly Ile Ala Leu Lys Met Gln Lys Arg Ser Thr Asn Lys Asn 435 440 445 Pro Ser Lys Val Gln Pro Val Phe Ala Asn Gly Ala Ala Thr Val Gly 450 455 460 Pro Glu Ala Ser Lys Ala Ser Ser Gly Ala Ser Ala Ser Ala Ser Ala 465 470 475 480 Ala Pro Ala Lys Pro Ala Phe Ser Ala Asp Val Leu Ala Pro Lys Pro 485 490 495 Val Ala Leu Pro Glu His Ile Leu Lys Gly Asp Ala Leu Ala Pro Lys 500 505 510 Glu Met Ser Trp His Pro Met Ser Lys Leu Pro Gly Asn Pro Thr Pro 515 520 525 Phe Phe Ser Pro Ser Ser Tyr Pro Pro Arg Ala Ile Cys Phe Ile Pro 530 535 540 Phe Pro Gly Asn Pro Leu Asp Asn Asn Cys Lys Ala Gly Glu Met Pro 545 550 555 560 Leu Asn Trp Tyr Asn Met Ser Glu Phe Met Cys Gly Lys Val Ser Asn 565 570 575 Cys Leu Gly Pro Glu Phe Ala Arg Phe Asp Lys Ser Asn Thr Ser Arg 580 585 590 Ser Pro Ala Phe Asp Leu Ala Leu Val Thr Arg Val Val Glu Val Thr 595 600 605 Asn Met Glu His Gly Lys Phe Leu Asn Val Asp Cys Asn Pro Ser Lys 610 615 620 Gly Thr Met Val Gly Glu Phe Asp Cys Pro Gln Asp Ala Trp Phe Phe 625 630 635 640 Asp Gly Ser Cys Asn Asp Gly His Met Pro Tyr Ser Ile Ile Met Glu 645 650 655 Ile Gly Leu Gln Thr Ser Gly Val Leu Thr Ser Val Leu Lys Ala Pro 660 665 670 Leu Thr Met Asp Lys Asp Asp Ile Leu Phe Arg Asn Leu Asp Ala Ser 675 680 685 Ala Glu Met Val Arg Pro Asp Val Asp Val Arg Gly Lys Thr Ile Arg 690 695 700 Asn Val Thr Lys Cys Thr Gly Tyr Ala Met Leu Gly Lys Met Gly Ile 705 710 715 720 His Arg Phe Thr Phe Glu Leu Ser Val Asp Gly Val Val Phe Tyr Lys 725 730 735 Gly Ser Thr Ser Phe Gly Trp Phe Thr Pro Glu Val Phe Ala Gln Gln 740 745 750 Ala Gly Leu Asp Asn Gly Lys Lys Thr Glu Pro Trp Cys Lys Thr Asn 755 760 765 Asn Thr Ser Val Arg Arg Val Glu Ile Ala Ser Ala Lys Gly Lys Glu 770 775 780 Gln Leu Thr Glu Lys Leu Pro Asp Ala Thr Asn Ala Gln Val Leu Arg 785 790 795 800 Arg Ser Glu Gln Cys Glu Tyr Leu Asp Tyr Leu Asn Ile Ala Pro Asp 805 810 815 Ser Gly Leu His Gly Lys Gly Tyr Ala His Gly His Lys Asp Val Asn 820 825 830 Pro Gln Asp Trp Phe Phe Ser Cys His Phe Trp Phe Asp Pro Val Met 835 840 845 Pro Gly Ser Leu Gly Ile Glu Ser Met Phe Gln Leu Ile Glu Ala Phe 850 855 860 Ala Val Asp Gln Asn Ile Pro Gly Glu Tyr Asn Val Ser Asn Pro Thr 865 870 875 880 Phe Ala His Ala Pro Gly Lys Thr Ala Trp Lys Tyr Arg Gly Gln Leu 885 890 895 Thr Pro Lys Asn Arg Ala Met Asp Cys Glu Val His Ile Val Ser Ile 900 905 910 Thr Ala Ser Pro Glu Asn Gly Gly Tyr Val Asp Ile Val Ala Asp Gly 915 920 925 Ala Leu Trp Val Asp Gly Leu Arg Val Tyr Glu Ala Lys Glu Leu Arg 930 935 940 Val Arg Val Val Ser Ala Lys Pro Gln Ala Ile Pro Asp Val Gln Gln 945 950 955 960 Gln Pro Pro Ser Ala Lys Ala Asp Pro Gly Lys Thr Gly Val Ala Leu 965 970 975 Ser Pro Thr Gln Leu Arg Asp Val Leu Leu Glu Val Asp Asn Pro Leu 980 985 990 Tyr Leu Gly Val Glu Asn Ser Asn Leu Val Gln Phe Glu Ser Lys Pro 995 1000 1005 Ala Thr Ser Ser Arg Ile Val Ser Ile Lys Pro Cys Ser Ile Ser 1010 1015 1020 Asp Leu Gly Asp Lys Ser Phe Met Glu Thr Tyr Asn Val Ser Ala 1025 1030 1035 Pro Leu Tyr Thr Gly Ala Met Ala Lys Gly Ile Ala Ser Ala Asp 1040 1045 1050 Leu Val Ile Ala Ala Gly Lys Arg Lys Ile Leu Gly Ser Phe Gly 1055 1060 1065 Ala Gly Gly Leu Pro Met His His Val Arg Ala Ala Leu Glu Lys 1070 1075 1080 Ile Gln Ala Ala Leu Pro Gln Gly Pro Tyr Ala Val Asn Leu Ile 1085 1090 1095 His Ser Pro Phe Asp Ser Asn Leu Glu Lys Gly Asn Val Asp Leu 1100 1105 1110 Phe Leu Glu Lys Gly Val Thr Val Val Glu Ala Ser Ala Phe Met 1115 1120 1125 Thr Leu Thr Pro Gln Val Val Arg Tyr Arg Ala Ala Gly Leu Ser 1130 1135 1140 Arg Asn Ala Asp Gly Ser Val Asn Ile Arg Asn Arg Ile Ile Gly 1145 1150 1155 Lys Val Ser Arg Thr Glu Leu Ala Glu Met Phe Ile Arg Pro Ala 1160 1165 1170 Pro Glu His Leu Leu Glu Lys Leu Ile Ala Ser Gly Glu Ile Thr 1175 1180 1185 Gln Glu Gln Ala Glu Leu Ala Arg Arg Val Pro Val Ala Asp Asp 1190 1195 1200 Ile Ala Val Glu Ala Asp Ser Gly Gly His Thr Asp Asn Arg Pro 1205 1210 1215 Ile His Val Ile Leu Pro Leu Ile Ile Asn Leu Arg Asn Arg Leu 1220 1225 1230 His Arg Glu Cys Gly Tyr Pro Ala His Leu Arg Val Arg Val Gly 1235 1240 1245 Ala Gly Gly Gly Val Gly Cys Pro Gln Ala Ala Ala Ala Ala Leu 1250 1255 1260 Thr Met Gly Ala Ala Phe Ile Val Thr Gly Thr Val Asn Gln Val 1265 1270 1275 Ala Lys Gln Ser Gly Thr Cys Asp Asn Val Arg Lys Gln Leu Ser 1280 1285 1290 Gln Ala Thr Tyr Ser Asp Ile Cys Met Ala Pro Ala Ala Asp Met 1295 1300 1305 Phe Glu Glu Gly Val Lys Leu Gln Val Leu Lys Lys Gly Thr Met 1310 1315 1320 Phe Pro Ser Arg Ala Asn Lys Leu Tyr Glu Leu Phe Cys Lys Tyr 1325 1330 1335 Asp Ser Phe Asp Ser Met Pro Pro Ala Glu Leu Glu Arg Ile Glu 1340 1345 1350 Lys Arg Ile Phe Lys Arg Ala Leu Gln Glu Val Trp Glu Glu Thr 1355 1360 1365 Lys Asp Phe Tyr Ile Asn Gly Leu Lys Asn Pro Glu Lys Ile Gln 1370 1375 1380 Arg Ala Glu His Asp Pro Lys Leu Lys Met Ser Leu Cys Phe Arg 1385 1390 1395 Trp Tyr Leu Gly Leu Ala Ser Arg Trp Ala Asn Met Gly Ala Pro 1400 1405 1410 Asp Arg Val Met Asp Tyr Gln Val Trp Cys Gly Pro Ala Ile Gly 1415 1420 1425 Ala Phe Asn Asp Phe Ile Lys Gly Thr Tyr Leu Asp Pro Ala Val 1430 1435 1440 Ser Asn Glu Tyr Pro Cys Val Val Gln Ile Asn Leu Gln Ile Leu 1445 1450 1455 Arg Gly Ala Cys Tyr Leu Arg Arg Leu Asn Ala Leu Arg Asn Asp 1460 1465 1470 Pro Arg Ile Asp Leu Glu Thr Glu Asp Ala Ala Phe Val Tyr Glu 1475 1480 1485 Pro Thr Asn Ala Leu 1490 <210> 4 <211> 780 <212> PRT <213> Schizochytrium sp. ATCC_20888 <220> <223> SzACS-2 v3 protein <300> <302> POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS <310> US 2007-0245431 A1 <311> 2007-03-15 <312> 2007-10-18 <400> 4 Met Pro Thr Pro Asp Ala Thr Ala Pro Leu Asn Lys Pro Ser Asp Tyr 1 5 10 15 Ala Val Tyr His Glu Glu Asp Gly Pro Phe Trp Thr Ala Asp Ser Ser 20 25 30 Gly Val Ser Arg Val Asn Phe Ser Glu Thr Gly Val Gly Ser Glu Gly 35 40 45 Val Ile Pro Ala Leu Thr Leu Ile Asp Val Phe Glu Arg Ala Val Lys 50 55 60 Arg Gly Gly Asn Arg Ile Ala Phe Arg Thr Glu Asn Met Pro Thr Leu 65 70 75 80 Arg Arg Gly Glu Glu Ala Pro Asp Ala Leu Pro Leu Lys Asp Trp Lys 85 90 95 Ser Trp Ser Trp Lys Gln Tyr Lys Ala Asp Val His Arg Ile Ala Arg 100 105 110 Ala Leu Met Asp Leu Gly Val Glu Gln His Asp Ala Val Ser Ile Phe 115 120 125 Gly Phe Asn Ser Pro Glu Trp Phe Leu Ser Ala Val Gly Ala Val His 130 135 140 Ala Gly Ala Lys Ile Ala Gly Ile Tyr Pro Ser Asp Thr Pro Ala Gln 145 150 155 160 Val Gln Tyr Lys Ala Phe His Ser Asp Thr Ala Val Ala Val Val Glu 165 170 175 Asn Glu Gln Cys Phe Lys Lys Phe Ala Glu Val Val Glu Asp Leu Pro 180 185 190 Tyr Leu Lys Ala Ile Val Cys Trp Asp Tyr Glu Ala Thr Asp Ile Thr 195 200 205 Arg Glu Asp Gly Ser Val Val Glu Val Leu Thr Phe Ala Glu Phe Leu 210 215 220 Lys Arg Gly Asp Thr Val Glu Ala Ala Ala Leu Asp Glu Arg Ile Ser 225 230 235 240 Lys Ile Glu Pro Thr Met Cys Ala Ala Leu Ile Tyr Thr Ser Gly Thr 245 250 255 Thr Gly Arg Pro Lys Ala Val Met Ile Ser His Asp Asn Leu Val Phe 260 265 270 Glu Ala Ser Ala Val Val Pro Asn Leu Gly Gly Ala Cys Thr Thr Thr 275 280 285 Ala Glu Glu Arg Ile Leu Ser Tyr Leu Pro Leu Ser His Val Ala Gly 290 295 300 Met Met Val Asp Ile Ile Ala Pro Ile Ile Ala Thr Ala Phe His Lys 305 310 315 320 Gly Arg Ile Cys Val Cys Phe Ala Arg Pro Tyr Asp Leu Arg Thr Gly 325 330 335 Thr Leu Gly Gln Arg Leu Asn Ala Val Glu Pro Thr Ile Phe Leu Gly 340 345 350 Val Pro Arg Val Trp Glu Lys Ile Gln Glu Lys Leu Met Ala Val Gly 355 360 365 Ala Lys Thr Thr Gly Leu Lys Lys Lys Leu Ser Thr Ala Ala Lys Lys 370 375 380 Arg Gly Leu Glu Phe Gln Glu Glu Gln Gln Ile Gly Arg Ser Gly Ala 385 390 395 400 Asn Pro Gly Phe Gly Pro Leu Gly Ile Tyr Lys Lys Leu Leu Gly Leu 405 410 415 Ile Lys Gly Lys Leu Gly Leu Thr Lys Cys Lys Phe Ala Phe Ala Gly 420 425 430 Ala Ala Pro Met Thr Arg Glu Thr Leu Gln Tyr Phe Gly Ala Leu Asn 435 440 445 Ile Asn Ile Asn Glu Val Tyr Gly Met Ser Glu Cys Ser Gly Ala Ala 450 455 460 Thr Trp Ser Thr Asp Lys Ala His Glu Trp Gly Thr Val Gly Tyr Glu 465 470 475 480 Met Pro Ser Cys Glu Val Arg Val Phe Lys Ile Ala Glu Asp Gly Thr 485 490 495 Lys Thr Glu Cys Pro Arg Ala Ala Asp Ile Met His Ala Thr Glu Glu 500 505 510 Glu Gln Gly Glu Val Cys Phe Arg Gly Arg Asn Ile Met Met Gly Tyr 515 520 525 Leu Ala Asn Pro Lys Leu Gly Asp Asp His Val Ala Glu Ile Glu Glu 530 535 540 Lys Asn Ala Ala Ala Ile Asp Ser Glu Gly Trp Leu His Ser Gly Asp 545 550 555 560 Lys Gly Ala Ile Ser Thr Arg Gly Met Leu Lys Ile Thr Gly Arg Tyr 565 570 575 Lys Glu Leu Ile Ile Gly Ala Gly Gly Glu Asn Val Ala Pro Val Pro 580 585 590 Ile Glu Asp Ala Ile Lys Ala Arg Met Pro Phe Val Ser Asn Ala Met 595 600 605 Met Val Gly Asp Lys Arg Lys Phe Met Ala Val Leu Leu Thr Leu Lys 610 615 620 Thr Val Gly Ala Thr Gly Glu Leu Pro Gly Thr Asn Lys Leu Met Gly 625 630 635 640 Ala Ala Ala Asp Tyr Gly Glu Thr Ile Glu Asp Ala Cys Asp Asn Glu 645 650 655 Ala Leu Ile Glu Glu Ile Thr Gln Gln Leu Lys Glu Ile Gly Asp Asp 660 665 670 Gly Asp Val Thr Pro Ser Asn Ala Ala Arg Ile Gln Lys Phe Thr Ile 675 680 685 Leu Pro Leu Asp Phe Ser Val Ser Thr Asp Glu Leu Thr Ala Thr Leu 690 695 700 Lys Leu Lys Arg Ser Val Val Ala Asp Lys Tyr Glu Asp Ile Ile Glu 705 710 715 720 Ala Phe Tyr Glu Ser Lys Ser Val Phe Val Pro Tyr Ser Thr Val Gly 725 730 735 Ala Tyr Ala Thr Gly Gly Pro Val Asp Asp Ser Val Val Asp Gly Ser 740 745 750 Phe Lys Gly Asp Phe Ser Met Ile Gly Asp Asp Asp Pro Asp Leu Gln 755 760 765 Asn Val Asp Val Leu Glu Ala Ile Asp Glu Asp Asn 770 775 780 <210> 5 <211> 237 <212> PRT <213> Nostoc sp. PCC 7120 <220> <223> NoHetI v3 protein <300> <308> GenBank: P37695 <309> 1994-10-01 <400> 5 Met Leu Gln His Thr Trp Leu Pro Lys Pro Pro Asn Leu Thr Leu Leu 1 5 10 15 Ser Asp Glu Val His Leu Trp Arg Ile Pro Leu Asp Gln Pro Glu Ser 20 25 30 Gln Leu Gln Asp Leu Ala Ala Thr Leu Ser Ser Asp Glu Leu Ala Arg 35 40 45 Ala Asn Arg Phe Tyr Phe Pro Glu His Arg Arg Arg Phe Thr Ala Gly 50 55 60 Arg Gly Ile Leu Arg Ser Ile Leu Gly Gly Tyr Leu Gly Val Glu Pro 65 70 75 80 Gly Gln Val Lys Phe Asp Tyr Glu Ser Arg Gly Lys Pro Ile Leu Gly 85 90 95 Asp Arg Phe Ala Glu Ser Gly Leu Leu Phe Asn Leu Ser His Ser Gln 100 105 110 Asn Leu Ala Leu Cys Ala Val Asn Tyr Thr Arg Gln Ile Gly Ile Asp 115 120 125 Leu Glu Tyr Leu Arg Pro Thr Ser Asp Leu Glu Ser Leu Ala Lys Arg 130 135 140 Phe Phe Leu Pro Arg Glu Tyr Glu Leu Leu Arg Ser Leu Pro Asp Glu 145 150 155 160 Gln Lys Gln Lys Ile Phe Phe Arg Tyr Trp Thr Cys Lys Glu Ala Tyr 165 170 175 Leu Lys Ala Thr Gly Asp Gly Ile Ala Lys Leu Glu Glu Ile Glu Ile 180 185 190 Ala Leu Thr Pro Thr Glu Pro Ala Lys Leu Gln Thr Ala Pro Ala Trp 195 200 205 Ser Leu Leu Glu Leu Val Pro Asp Asp Asn Cys Val Ala Ala Val Ala 210 215 220 Val Ala Gly Phe Gly Trp Gln Pro Lys Phe Trp His Tyr 225 230 235 <210> 6 <211> 8733 <212> DNA <213> Schizochytrium sp. ATCC_20888 <220> <223> SzPUFA OrfA v3 gene <300> <308> GenBank: AF378327 <309> 2007-10-22 <400> 6 atggcagcga ggctgcaaga gcagaaagga ggtgagatgg acacgaggat tgcgatcata 60 ggcatgagcg ccatacttcc ttgtggaacc acggtgagag agtcatggga gaccataagg 120 gctggcattg attgcctgtc tgatcttcct gaggatcgtg ttgatgtgac tgcgtacttt 180 gacccagtca agaccacaaa ggacaaaatc tactgcaaga gaggaggttt cattcctgag 240 tatgactttg atgcgcgtga gtttggtctc aacatgttcc agatggaaga ttctgatgcg 300 aatcagacca tctcacttct caaggtcaaa gaagcacttc aagatgctgg gattgatgca 360 cttggaaagg agaagaaaaa cataggatgc gtgcttggca ttggaggtgg acagaagagt 420 tcacatgagt tctacagcag actcaactat gtggttgtgg agaaagtctt gagaaagatg 480 gggatgccag aggaagatgt caaagtggct gtggagaagt acaaagcgaa cttcccagaa 540 tggagacttg actctttccc tggattcctt gggaatgtca ctgctggcag atgcaccaac 600 acattcaact tggatggcat gaactgcgtt gtggatgctg catgtgcaag ctccttgata 660 gcagtcaagg ttgcgattga tgaactgctc tatggtgact gcgacatgat ggtcactggt 720 gcaacctgca cagacaactc cattgggatg tacatggctt tctcaaagac acctgtgttc 780 agcacggacc caagtgtgag agcgtatgat gagaaaacga aagggatgct cattggtgag 840 ggatcagcca tgttggtgct caaacgttat gcagatgcag tgagggatgg tgatgagatt 900 catgctgtga ttcgtggttg tgcaagctca agtgatggca aagcagctgg catctacact 960 cccacaatca gtggtcaaga ggaagccttg agacgtgcat acaatcgtgc ctgcgttgac 1020 cctgccactg tcactttggt tgagggtcat gggactggca ctccagttgg agataggatt 1080 gagttgactg cgttgaggaa cttgtttgac aaagcgtatg gtgaagggaa cacagagaaa 1140 gttgcagttg gaagtatcaa gtccagtatt ggtcatttga aggctgttgc tggtcttgct 1200 ggaatgatca aggtcatcat ggctctcaaa cacaagactt tgcctggaac gatcaatgtg 1260 gacaatccac ccaatctcta tgacaacact cccatcaatg agtcatccct ctacatcaac 1320 acgatgaaca gaccctggtt ccctccacct ggtgttccga gacgtgctgg aatatccagc 1380 tttggctttg gaggtgccaa ctaccatgca gtgttggaag aggctgaacc agagcacacg 1440 acagcgtaca gactcaacaa acgtccacaa ccggtgttga tgatggctgc aactcctgct 1500 gcgttgcaga gcctttgtga agctcagctg aaagagtttg aagctgcgat caaggagaat 1560 gagacagtca agaatactgc ctacatcaag tgtgtcaagt ttggagaaca gttcaagttc 1620 cctgggagca tacctgcgac caatgcgaga ctgggattct tggtcaaaga tgcggaggat 1680 gcgtgctcca cgcttcgtgc tatatgtgct cagtttgcca aagatgtgac caaagaagcg 1740 tggaggcttc cgagagaagg agtttctttc cgtgccaaag gcattgccac caatggtgct 1800 gttgctgcac tcttctctgg acaaggtgct cagtacacac acatgttcag tgaagttgcg 1860 atgaactggc ctcagttcag acagtccatt gctgcaatgg acgctgcaca gtccaaggtt 1920 gctggaagtg acaaagactt tgaaagagtc tctcaagtcc tgtacccaag gaaaccgtac 1980 gaacgtgagc cagaacaaga tcacaagaaa atctcactca cagcatacag ccagccgtca 2040 accctggcat gtgctcttgg tgcctttgag attttcaagg aagctggatt cactccagac 2100 tttgcagctg gacacagctt gggtgagttt gctgcgctct atgcagctgg ttgtgttgac 2160 agagatgagt tgtttgaact tgtttgcaga agggcaagga tcatgggagg caaagatgca 2220 cctgccacac ccaaaggttg catggcagct gtcataggac ccaacgcaga gaacatcaag 2280 gttcaagctg ccaacgtctg gcttggaaac tcaaactctc cgtctcagac tgtcattact 2340 gggtctgttg aagggattca agctgaaagt gccagattgc agaaggaagg gttcagagtt 2400 gtgcctcttg cttgtgagtc tgccttccat tctccacaga tggaaaatgc cagctcagct 2460 ttcaaagatg tcatatccaa ggtttccttc cgtacaccca aagcagaaac aaagctcttc 2520 agcaatgttt ctggagagac ctatccgacg gatgccagag agatgctcac tcaacacatg 2580 acctcatctg tgaagttcct gacccaagtg aggaacatgc accaagctgg tgcgagaatc 2640 tttgttgagt ttggaccgaa acaagttctc agcaaacttg tttctgaaac cctcaaagat 2700 gacccgtctg ttgtcacagt gtctgtgaac ccagcttctg gcacggactc tgacatacag 2760 ttgagggatg ctgcagttca gctggttgtg gctggtgtca atcttcaagg gtttgacaag 2820 tgggatgctc ctgatgccac gaggatgcaa gccatcaaga aaaagagaac cacactcaga 2880 ctttcagctg ccacctatgt cagtgacaag accaagaaag tgagggatgc tgccatgaac 2940 gacggaagat gtgtcacata cctcaagggt gctgcaccac tcatcaaggc tcctgaacct 3000 gtggttgacg aagctgccaa gagggaggct gaaaggcttc agaaggagct tcaagacgct 3060 cagaggcagc ttgacgacgc caaacgtgca gctgcagaag ccaactccaa acttgctgct 3120 gccaaggagg aagccaagac tgctgcagct tcagccaagc ctgcagttga cactgctgtt 3180 gtggagaaac atcgtgccat cctcaagtcc atgctggctg agctggatgg ttatggaagt 3240 gttgatgctt cttcccttca gcaacagcaa cagcaacaga ctgctccggc gcctgttaaa 3300 gccgctgccc ctgccgctcc cgttgcaagt gctcctgctc ccgccgtgtc caacgagttg 3360 cttgagaaag ctgagacagt tgtgatggag gttctcgctg ccaagactgg atacgaaaca 3420 gacatgattg aggcggacat ggagcttgag actgagttgg gaattgacag catcaagagg 3480 gttgaaattc tttctgaagt ccaagctatg cttaacgttg aggcgaaaga tgttgatgct 3540 ctctctagga cgagaacagt gggtgaagtt gtcaacgcta tgaaggcaga aatcgctggt 3600 tcttccgctc cggcgccggc agctgccgca cctgcaccgg ctaaagcggc tccagctgca 3660 gctgcaccag ctgtttctaa tgagctgctc gaaaaggcag aaactgttgt gatggaagtc 3720 cttgctgcca aaactggcta cgaaactgac atgatagaat cagacatgga gcttgagacg 3780 gagcttggaa ttgattccat caagcgtgtg gaaatcctca gtgaagtcca agccatgctc 3840 aatgtggaag ctaaggatgt ggatgctctc tcaaggacca gaactgtcgg tgaggttgtc 3900 aatgccatga aggctgagat agctggaggc tccgctcctg cgccggccgc tgccgctcct 3960 ggtcccgctg ccgcggctcc agctccagct gcagccgctc cggccgtctc aaacgaactc 4020 cttgaaaaag ctgagaccgt cgtgatggag gtcttggcag ctaaaaccgg ctatgagact 4080 gacatgattg agtctgacat ggaattggag acagaacttg ggatagacag catcaaacgt 4140 gttgaaatcc tcagtgaggt ccaagctatg ctgaacgtgg aagcgaagga tgttgatgct 4200 ctgtccagaa cgaggaccgt tggtgaggtt gtggatgcca tgaaggctga aattgctgga 4260 gggtctgcgc ccgcgccagc tgcagccgct cctgcacccg cagccgcggc acctgctcca 4320 gctgcacctg cacctgccgt ttcttcagaa ctcttggaga aggctgagac ggttgtgatg 4380 gaggttttgg ctgcaaagac tggctatgag accgacatga ttgaatctga tatggaattg 4440 gagacagagc ttgggatcga ttccatcaaa agagtggaga tcctgagtga agtccaagcc 4500 atgctcaatg ttgaagctaa ggatgttgat gcactttcaa gaacaaggac agtgggtgag 4560 gttgtggatg ccatgaaggc ggagattgct ggagggagcg cgcctgcgcc agctgccgcg 4620 gcacctgcac cggcagccgc agctccagct ccagctgcac cagcaccggc cgcgcccgcg 4680 ccagctgttt catccgaact cctggaaaag gccgagacgg ttgtgatgga ggtgctcgca 4740 gctaagactg gttacgagac cgacatgatt gaaagtgaca tggagcttga aaccgagttg 4800 ggaatagata gcatcaaacg tgttgaaatc ttgtctgagg tccaagctat gttgaacgtg 4860 gaggcaaaag atgtcgatgc gctttcaaga accagaacag tcggtgaggt cgtggacgcc 4920 atgaaggctg aaattgccgg aagctctgct agtgcgccag ctgcagccgc acctgcacct 4980 gctgccgcgg cacctgctcc agctgcagcc gctcctgccg tcagcaatga gttgctggag 5040 aaagccgaaa cagttgtgat ggaagtcctt gctgcaaaaa ctggttacga aacggacatg 5100 attgagtcag acatggaatt ggagaccgaa cttggaatag attccatcaa gagagtggag 5160 atcctctctg aggtgcaagc catgctcaac gttgaggcta aagatgttga tgcactcagt 5220 aggacaagaa ctgtggggga agttgtcgat gcgatgaagg ctgagattgc tggaggctct 5280 gctcctgcgc cggcagctgc cgcacctgca cccgcagccg cggcaccagc tgtcagcaac 5340 gaattgcttg agaaggctga aacagttgtc atggaagtgc tggctgcaaa aaccggctat 5400 gagactgaca tgattgagtc tgacatggaa cttgagacgg aattgggcat tgacagcatc 5460 aagcgtgttg agatcctcag tgaagtccaa gcaatgctca atgtggaggc taaggatgtg 5520 gatgccctct caaggaccag aacagttggt gaggtcgttg atgcgatgaa ggcggagatc 5580 gctggaagtt ccgcgcctgc gccagctgca gccgctcctg cacccgcagc cgcggcaccg 5640 gctccagctg cagccgcacc ggccgtctcc tcagaactcc tggaaaaggc tgagacagtt 5700 gtgatggaag tccttgctgc gaaaactggc tatgagaccg acatgattga atctgacatg 5760 gaactggaga ccgagcttgg aattgactcc atcaaacgtg ttgaaatcct ctctgaggtt 5820 caagcgatgt tgaatgtgga ggccaaagat gttgatgctc tttccagaac aaggacggtg 5880 ggagaggtgg ttgatgccat gaaggctgag atagctgggg gttctgctcc tgcgccagct 5940 gcagccgctc ctgctcccgc tgccgcggca ccagctgtca gcaacgaact ccttgaaaaa 6000 gctgaaactg ttgtgatgga ggttttggct gcaaagactg gatacgagac tgacatgata 6060 gagtcagaca tggagttgga aacagagctt gggattgata gcatcaagcg tgtggaaatc 6120 ctttctgagg ttcaagccat gctgaacgtt gaggccaaag atgtcgatgc tttgtcaagg 6180 accagaacgg ttggagaagt ggtcgatgcc atgaaggctg agatagctgg tggatcagcc 6240 cctgctccgg ctgcagccgc tcctgcaagt gccggtgctg caccagctgt gaagattgat 6300 tcagttcatg gtgctgactg tgacgatttg tcactgatgc acgcaaaggt ggttgacatc 6360 agaaggccag atgaactgat tctggaaaga ccggagaacc gtccagttct tgtcgttgac 6420 gatggctcag agttgacact tgctcttgtg agggtgttgg gagcctgtgc agttgtcttg 6480 acctttgagg gattgcagtt ggctcagaga gctggtgcag ctgccattcg tcatgttctg 6540 gctaaggacc tttcagcaga atcagctgag aaggccatca aagaggctga gcaaagattt 6600 ggagcgttgg gaggcttcat aagccagcaa gcagaaaggt ttgaaccagc tgagatactg 6660 ggtttcacac tcatgtgtgc gaagtttgcc aaagcctctc tctgcactgc tgtggctgga 6720 ggtcgtcctg ccttcatcgg tgtggctagg cttgacggca gacttggatt cacctctcaa 6780 ggcacttctg acgcactcaa gagagcacag cgtggagcga tctttggact ctgcaagaca 6840 atagggttgg agtggtcaga atctgatgtg ttcagccgtg gtgtggacat tgctcaaggg 6900 atgcacccag aagatgctgc agttgccata gttagggaga tggcgtgtgc tgacatcaga 6960 atcagagagg ttgggattgg agcaaaccag caaagatgca caatcagagc tgccaagttg 7020 gagactggga accctcagag acagatagcg aaagacgatg tgcttctggt ttctggaggt 7080 gctcgtggga tcacacctct ttgcatccgt gagatcacgc gtcagattgc tggaggcaag 7140 tacattctgt tgggtcgtag caaagtttct gcgtctgaac cagcatggtg tgctggcatc 7200 actgatgaga aggctgtgca gaaagctgcc acccaagagt tgaagagggc cttcagtgct 7260 ggtgaaggac ccaaacccac tccaagggct gtcacgaaac ttgtgggatc tgttcttggt 7320 gctcgtgaag tgagaagctc cattgctgcc attgaagctc tgggtggcaa agcgatctac 7380 agctcttgtg atgtgaactc tgctgcagat gttgccaaag cagttaggga tgcagagtcc 7440 cagcttggtg ccagagttag tggaatcgtt cacgcttctg gtgtgctccg tgatcgtctc 7500 atagagaaga aactccctga cgagtttgat gctgtctttg ggaccaaagt cactgggttg 7560 gagaatcttc tggctgcagt tgatcgtgcc aacctcaaac acatggttct cttcagctcc 7620 cttgctggtt tccacggcaa tgttggtcag tctgactatg cgatggcaaa cgaagctctc 7680 aacaagatgg gtttggaact tgcgaaggat gtcagtgtca agtccatctg ctttggtccc 7740 tgggatggtg ggatggtgac acctcaactc aagaaacagt ttcaagaaat gggagttcag 7800 atcattcctc gtgaaggagg tgctgataca gttgcgagga ttgtgttggg atcaagtcct 7860 gctgagatcc ttgttggaaa ctggaggaca ccatccaaga aagttggaag tgacacgatc 7920 accctgcata gaaagatcag tgccaagtcc aatcccttct tggaggatca tgtcatacaa 7980 ggcagaaggg tgttgccaat gacccttgct attgggagcc ttgctgagac ctgccttgga 8040 ctcttccctg gctactctct ctgggccata gatgacgcac agctcttcaa aggtgtcact 8100 gttgatggag acgtcaactg tgaagtgacc ctcacaccgt ccactgctcc gagtgggagg 8160 gtgaatgtcc aagcgacgct caagacgttc tcaagtggaa aacttgttcc agcctacaga 8220 gctgtcatag tgttgtccaa ccaaggtgct ccacctgcca atgcaaccat gcagcctccg 8280 agccttgatg cagatcctgc tttgcaaggg tcagtctatg atggcaagac tctctttcat 8340 ggtcctgcgt tcagaggcat tgatgacgtt ctcagctgca ccaagagcca gcttgttgcc 8400 aagtgttctg ctgttcctgg atcagatgca gccagaggag agtttgcaac tgacactgat 8460 gcacatgatc cctttgtcaa tgatcttgcc tttcaagcaa tgttggtctg ggtgaggaga 8520 acgctgggac aagctgcact cccgaactcc attcagagga tagtccaaca cagaccggtc 8580 ccacaagaca aacccttcta catcaccctc agatccaatc agagtggtgg acatagccag 8640 cacaaacatg cacttcagtt ccacaacgaa caaggtgatc tcttcattga tgttcaagcc 8700 tctgtcattg cgacagactc acttgcgttc tga 8733 <210> 7 <211> 6180 <212> DNA <213> Schizochytrium sp. ATCC_20888 <220> <223> SzPUFA OrfB v3 gene <300> <308> GenBank: AF378328 <309> 2007-10-22 <400> 7 atggctgcaa ggaacgtgag tgcggctcat gagatgcatg acgagaagag gatagctgtt 60 gtgggcatgg ctgttcagta tgctggatgc aaaacgaaag acgagttctg ggaagttctc 120 atgaatggaa aagttgagag caaagtgatc agcgacaaac gtcttggttc aaattaccgt 180 gctgaacact acaaagctga aaggagcaag tatgctgaca ccttctgcaa tgagacatat 240 ggaactcttg atgagaacga aatagacaat gagcatgaac tccttttgaa ccttgccaaa 300 caagccttgg ctgaaacatc tgtcaaagat tcaacaagat gtggcatagt ttctggatgc 360 ctcagcttcc cgatggacaa cctccaaggt gaactcttga atgtgtacca gaaccatgtt 420 gagaagaaac ttggtgccag agttttcaaa gatgcctctc actggtcaga aagagaacag 480 tccaacaaac cagaggctgg tgacagaaga atcttcatgg accctgcttc ctttgttgct 540 gaggaactca atcttggcgc tctccactac tctgttgatg ctgcctgtgc cactgcgctc 600 tatgtgctca gactggcgca agatcacttg gtctctggtg cagctgatgt gatgctttgt 660 ggtgcaacat gcctgccgga gccgtttttc atactctctg ggttctcaac cttccaagcc 720 atgcctgttg gaacgggtca gaatgtgtcc atgccgctgc acaaagattc tcaaggattg 780 acccctggtg aaggaggttc catcatggtc ctcaaacgtt tggatgacgc aatcagagat 840 ggagatcaca tctatggaac acttttggga gccaatgtca gcaacagtgg cactggtctg 900 ccacttaaac cccttttgcc gtcagagaaa aagtgcctca tggacaccta cacacgtatc 960 aatgtgcatc cccacaagat tcagtatgtt gaatgccatg caactgggac tccacaaggt 1020 gatcgtgtgg aaattgatgc tgtgaaggct tgctttgaag gcaaagtccc acgttttgga 1080 accacaaaag ggaactttgg acacacattg gtggcagctg ggtttgctgg catgtgcaaa 1140 gtgcttctct ccatgaagca cgggatcatt ccacccactc cgggaataga tgacgaaacg 1200 aagatggacc ctctggttgt ctctggtgag gccattccct ggccagaaac gaatggagaa 1260 ccgaagcgtg ctggtctctc agcgtttgga tttggtggga ccaatgctca tgctgtcttt 1320 gaggaacatg acccaagcaa tgctgcatgc actggccatg actccatttc tgctctctct 1380 gcacgttgtg gtggtgagtc aaacatgaga atagcaatca ctgggatgga tgccactttt 1440 ggtgctttga aaggattgga tgcttttgaa agggccatct acactggtgc tcatggtgct 1500 ataccactcc cagaaaagag atggagattc ctggggaaag acaaggactt ccttgatctt 1560 tgtggagtca aagcaacacc gcatggctgt tacattgaag atgttgaagt tgatttccag 1620 aggctgagga ctccaatgac tcctgaagat atgctcttgc ctcaacagct tctggctgtc 1680 accacaattg atagggccat attggactct ggcatgaaga aaggtggaaa tgtggctgtc 1740 tttgtggggt tggggaccga tcttgaactc taccgtcacc gtgcaagggt tgctctcaag 1800 gagagagtga ggcctgaagc cagcaagaaa ctcaatgaca tgatgcagta catcaatgat 1860 tgtgggacaa gcacttctta caccagctac ataggcaact tggttgcaac aagggtcagt 1920 tcacaatggg gcttcacggg accttccttc acaatcacag aaggcaacaa ttctgtgtac 1980 agatgtgctg aacttggcaa atacctcttg gaaacgggag aggttgatgg ggttgtggtt 2040 gcgggtgttg atctctgtgg gagtgctgag aacctttacg tcaagtcaag gcgtttcaag 2100 gtgtctacat ctgacacccc gagagccagc tttgatgcag ctgctgatgg ttactttgtt 2160 ggagagggct gtggtgcatt tgtgctcaag cgtgagacaa gctgcaccaa ggatgacaga 2220 atctatgctt gcatggatgc catagttcct ggaaatgttc catctgcatg tttgagagag 2280 gcattggatc aagccagagt caagcctgga gacatcgaga tgttggagct ttctgctgat 2340 tcagcgaggc atttgaagga cccgtctgtt ctcccaaagg aactcactgc tgaggaagag 2400 attggcggac ttcagacgat tcttcgtgac gatgacaagc tcccaaggaa tgttgcaact 2460 ggaagtgtca aggccactgt tggtgacact ggttatgctt ctggtgctgc cagtttgatt 2520 aaggctgcgc tttgcatcta caaccgttac ctgcccagca atggagatga ctgggatgaa 2580 cctgcacctg aagcaccctg ggacagcaca ctctttgcgt gccagacctc aagagcttgg 2640 ttgaagaacc ctggtgagag acgttatgcg gctgtttccg gagtcagtga aaccagaagt 2700 tgctacagtg tgctgctttc tgaagcagag ggacactacg agagagagaa cagaatcagt 2760 ttggatgagg aagcacccaa gctcattgtg ttgagagcag actcccatga agagattctt 2820 ggaagattgg acaagataag ggaaaggttc ttgcagccca cgggtgctgc acccagagag 2880 tctgaattga aggcgcaagc ccgtaggatc tttcttgaac tccttggtga gacccttgcc 2940 caagatgcag cttcatctgg aagtcagaag ccccttgctc tcagcttggt ctccactcca 3000 tccaagctcc agagagaggt ggaacttgcg gcaaaaggca ttccgagatg tctcaagatg 3060 agacgtgatt ggagttcccc tgctggaagc agatatgcgc ctgagccttt ggccagtgac 3120 agagttgcct tcatgtatgg agaaggaagg agcccttact atggcatcac acaagacatt 3180 cataggattt ggcctgaact tcatgaggtc atcaatgaga aaacaaaccg tctctgggct 3240 gaaggtgaca gatgggtcat gccaagggcc agcttcaaga gtgagcttga gtcacaacag 3300 caagagtttg acagaaacat gattgaaatg ttccgtcttg gaatcctcac ctcaattgcc 3360 ttcacaaacc tggcgagaga tgttctcaac atcacgccca aagcagcctt tggactcagt 3420 ttgggagaga tttccatgat ctttgctttc tccaagaaaa acggtttgat aagcgatcag 3480 ttgacaaagg atttgagaga gtcagatgtc tggaacaaag ctctggctgt tgagttcaat 3540 gcgctcagag aagcctgggg cattccacag tctgtcccaa aagatgagtt ctggcaaggt 3600 tacatagtga ggggaacgaa acaagacata gaagcagcca tagcaccaga ctcaaagtat 3660 gtgaggttga ccatcatcaa tgatgccaac actgctctca tcagtggaaa gcctgatgca 3720 tgcaaagctg cgattgctcg tcttggtggc aacatccctg cgcttccagt cactcaaggc 3780 atgtgtgggc attgcccaga ggtgggacct tacaccaagg acatcgcaaa gatccacgcc 3840 aaccttgagt tccctgtggt tgatggtctt gatctctgga ctaccataaa ccagaagagg 3900 cttgtgccaa gagccacggg tgccaaagat gaatgggctc cttcatcctt tggggagtat 3960 gctggacagc tctatgagaa acaagccaac ttccctcaga tagttgaaac catctacaag 4020 cagaactatg atgtgtttgt tgaagttggg ccaaacaatc acagaagcac agcagtgaga 4080 accacgcttg gaccacagag aaaccacctt gctggtgcca tagacaagca gaacgaggat 4140 gcttggacga caatagtcaa gctcgtggct tcattgaagg ctcaccttgt tcctggagtg 4200 accatcagtc cgctctacca ctccaagctc gttgcagagg ctgaggcttg ttatgctgcg 4260 ctctgtaaag gtgagaaacc caagaaaaac aagtttgtga gaaagattca gttgaatggc 4320 agattcaaca gcaaggcaga ccccatctcc tcagcagacc ttgcatcctt cccacctgcg 4380 gaccctgcga ttgaagctgc catctcttca aggatcatga agcctgtggc tccgaaattc 4440 tatgcgcgtc tcaacattga tgagcaagat gagaccagag atccgatctt gaacaaggac 4500 aatgcgcctt ccagctccag ctcttccagc agttcctctt ccagcagttc atctccgtct 4560 cctgctcctt cagctccagt tcagaagaaa gctgccccag ctgcggagac aaaagctgtt 4620 gccagtgctg atgcacttcg tagtgccctg cttgatcttg actccatgct tgcgctcagc 4680 tctgcaagtg cttctggcaa ccttgttgag actgcgccat ctgatgccag tgtcattgtc 4740 cctccgtgca acattgctga tcttggcagc agagctttca tgaaaactta tggtgtttct 4800 gcgcctctgt acaccggtgc aatggccaag ggaattgcct ctgcggactt ggtgatcgca 4860 gctggaaggc aaggaatcct tgcgtcattt ggtgctggag gccttccaat gcaagttgtg 4920 cgtgagtcca ttgaaaagat tcaagctgcc ctgcccaatg gaccatatgc tgtcaacctc 4980 atccattctc cttttgacag caaccttgag aagggaaatg ttgatctctt cttggaaaag 5040 ggtgtcactt ttgttgaggc ttcagccttc atgacgttga caccgcaagt tgtcagatac 5100 agagcggctg ggttgacgag gaatgcagat gggtcagtca acataaggaa cagaatcatt 5160 gggaaagtct ccagaactga gctggctgaa atgttcatgc gtcctgcacc tgagcatctt 5220 ttgcagaagc tcattgcttc tggggagatc aatcaagaac aagctgagtt ggccagacgt 5280 gttcctgtgg ctgatgacat tgcggttgaa gctgattctg gtggacacac tgacaaccgt 5340 ccaatccatg tcattctgcc tctgattatc aacctgaggg ataggcttca cagagagtgt 5400 ggatacccag caaacctgag ggttcgtgtt ggtgctgggg gtggcattgg gtgtccccaa 5460 gctgcgctgg caactttcaa catgggtgcc tctttcattg tcactggaac agtgaaccaa 5520 gttgccaaac aatctgggac gtgtgacaat gtgaggaaac agcttgcgaa agcaacttac 5580 tcagatgttt gcatggcacc ggcagctgac atgtttgagg aaggtgtgaa acttcaagtt 5640 ttgaagaaag gaacaatgtt cccttcaaga gccaacaaac tctatgagtt gttctgcaag 5700 tatgattctt ttgagtccat gcctccagca gagcttgcga gggttgagaa aagaatcttc 5760 agtcgtgcat tggaagaggt ctgggatgag accaagaact tctacatcaa ccgtctccac 5820 aacccagaga agatacagag ggctgagagg gaccccaaac tgaagatgtc actgtgtttc 5880 agatggtatc tgtccctggc ttcaaggtgg gccaacactg gagcttctga cagagtcatg 5940 gactaccaag tttggtgtgg tcctgccatt ggttcattca atgatttcat caaaggaact 6000 taccttgatc cggcagtggc aaatgagtac ccgtgtgttg tgcagatcaa caaacagatt 6060 cttagaggag cgtgcttctt gaggcgtctt gaaatactga gaaatgcaag gctttcagat 6120 ggtgcagctg cacttgtggc gagcattgat gacacatatg tccctgctga gaagctgtga 6180 <210> 8 <211> 4482 <212> DNA <213> Artificial sequence <220> <223> Chimeric PUFA OrfC from Schizochytrium sp. ATCC_20888 and Thraustochytrium <220> <223> hSzThPUFA OrfC v3 gene <300> <302> CHIMERIC PUFA POLYKETIDE SYNTHASE SYSTEMS AND USES THEREOF <310> US 2008-0022422 A1 <311> 2007-05-16 <312> 2008-01-24 <400> 8 atggcgcttc gtgtcaagac caacaagaag ccatgttggg agatgaccaa ggaggaactg 60 acctctggca aaactgaggt gttcaactat gaggaactcc tggagtttgc agagggtgac 120 atagctaagg tttttggtcc ggagtttgct gtcatagaca agtaccccag aagggtgagg 180 ctgcctgctc gtgaatacct gcttgtgaca agggtcacac tcatggatgc cgaggtcaac 240 aactacagag ttggtgccag aatggtcact gagtatgatc ttccagtgaa tggcgaattg 300 tctgagggag gtgattgtcc ctgggcagtt ctggttgaga gtggacagtg cgatttgatg 360 ctgatatcat acatgggaat tgatttccag aaccaaggtg acagagttta ccgtctgctc 420 aacaccacgt tgacattcta tggggttgcc catgagggag agacccttga gtatgacatc 480 agagtgaccg gttttgccaa acgtttggat ggtggcatct ccatgttctt ctttgagtat 540 gactgctatg tcaatgggag gcttctcatt gagatgagag atggctgtgc tggcttcttc 600 acaaatgagg agcttgatgc gggaaagggt gtggtgttca caagagggga tttggctgcc 660 agagctaaga ttccaaagca agatgtttct ccctatgctg tcgctccctg ccttcacaaa 720 acaaaactca acgaaaagga gatgcagacc ttggttgaca aagattgggc ttccgttttt 780 ggctcaaaga atggcatgcc ggagatcaac tacaaactct gcgcacgtaa gatgctcatg 840 attgatagag tcaccagcat tgaccataag ggaggtgtgt acggtttggg tcagttggtt 900 ggtgagaaaa tcttggagag ggaccattgg tacttcccat gccattttgt taaggatcaa 960 gtcatggctg gatctcttgt cagcgacggt tgttcacaaa tgctcaagat gtacatgatc 1020 tggctcggtt tgcaccttac caccggacca tttgacttca gacccgtcaa cggacacccg 1080 aacaaagtta ggtgtagggg tcagatatct cctcataagg gaaagttggt ctacgtcatg 1140 gagatcaaag agatgggatt cgacgaggat aacgatccgt acgccattgc tgacgtcaac 1200 atcattgacg ttgatttcga aaagggtcaa gatttcagcc ttgatagaat cagcgactac 1260 ggaaaggggg atcttaacaa aaagatcgtc gtggacttca agggcattgc tctcaagatg 1320 cagaagagat ccacgaacaa gaacccctcc aaagttcagc ccgtttttgc taacggtgca 1380 gccactgtgg gaccggaagc gtccaaggcc agctctggtg ccagtgctag tgcttctgct 1440 gcccctgcga agcctgcttt ctcagcagat gttcttgcgc caaagccagt tgcacttcct 1500 gaacatatcc tcaagggtga cgctctcgct ccgaaagaaa tgtcctggca tcccatgtca 1560 aaacttcctg gcaacccgac gccgttcttc tcccctagtt cttaccctcc gagggcaatt 1620 tgcttcatcc ctttccctgg caacccactt gacaacaatt gcaaagctgg agaaatgcct 1680 ctgaactggt acaacatgtc agagttcatg tgtggaaaag ttagcaactg cttgggacca 1740 gagttcgcaa gatttgataa gtcaaatacg agcagaagcc ctgcttttga tttggctctt 1800 gtgactagag ttgttgaagt cacaaacatg gaacatggca agttcctgaa tgttgattgc 1860 aatcccagca aaggcacaat ggtgggggag tttgactgtc ctcaagatgc gtggttcttt 1920 gatggttctt gcaatgatgg gcacatgcct tattccatca tcatggaaat cggacttcag 1980 acatccggtg ttctcaccag tgtgttgaaa gcaccactga cgatggacaa agatgacatt 2040 ctctttagga accttgatgc aagtgctgaa atggtgaggc cagatgtgga tgtgaggggg 2100 aaaaccataa ggaatgtgac caagtgcact ggatatgcaa tgttgggaaa gatggggatt 2160 cacagattca cgtttgagtt gtctgttgat ggcgtggttt tctacaaagg aagcacttcc 2220 tttggatggt tcactccaga ggtgtttgct cagcaagctg gacttgacaa tgggaaaaag 2280 acggagcctt ggtgcaaaac taacaacacg agtgtgagaa gagttgaaat cgcatcagcc 2340 aaaggcaaag aacagctgac tgagaaactt ccagatgcaa ctaatgctca agttctcaga 2400 aggtcagaac agtgtgagta tttggattac ctcaacattg ctcctgattc tgggctgcat 2460 gggaaaggat atgcgcatgg acacaaagat gttaacccac aagactggtt cttctcttgt 2520 cacttttggt ttgatcctgt gatgcctgga tctttgggaa ttgagtcaat gttccagctc 2580 attgaggcat ttgcggtgga ccagaacata ccgggagaat acaatgtgag caatccgacc 2640 tttgcccatg cccctggaaa aacggcgtgg aagtaccgtg gtcaattgac accaaagaac 2700 cgtgcgatgg actgtgaggt gcacatagtt tcaatcacag cctctcctga aaatggaggc 2760 tacgttgaca tagtggcaga tggagcactt tgggttgatg gtctccgtgt gtatgaagcc 2820 aaagaactca gagttcgtgt tgtcagtgca aaacctcaag ccattccgga tgttcaacaa 2880 cagccaccca gcgcaaaagc ggaccctggg aaaactggag ttgcactcag tccgactcag 2940 ttgagagatg tcctgcttga agtggacaat ccattgtatc ttggtgttga aaactccaat 3000 ttggtgcagt ttgaatccaa acctgcaact tcttcaagga ttgtcagtat caaaccatgc 3060 tccataagtg atcttggtga caagtccttc atggaaacgt acaatgtgtc agcacctctg 3120 tacactggag caatggccaa gggaattgcg tctgcggacc ttgtcattgc tgctgggaag 3180 aggaagatat tgggttcttt tggagcgggt gggcttccaa tgcaccatgt gagggcagct 3240 ttggagaaga tacaagcggc tctgcctcaa ggaccatatg ctgtgaacct cattcattca 3300 ccttttgaca gcaacttgga aaaggggaat gttgatctct tcctcgaaaa gggcgttact 3360 gtggtggagg cttctgcatt catgaccctc actccccaag ttgttcgtta ccgtgcagcc 3420 ggactctcaa ggaacgctga tgggagtgtc aacatcagaa accgtatcat tggaaaagtg 3480 tccagaacag aactcgccga aatgttcatc agaccggctc cggaacacct ccttgagaaa 3540 ctcatagctt ctggtgagat aacacaagaa caagccgaac ttgcccgtcg tgttcctgtc 3600 gcagacgaca tcgctgttga agctgattct ggtggtcaca ctgacaaccg tcccatccac 3660 gtcatcttgc ctttgatcat caaccttagg aacagactgc acagagagtg tggttatcct 3720 gcgcacctta gagtcagagt tggagctgga ggcggtgttg gctgcccaca agctgctgcg 3780 gctgccctca caatgggagc tgccttcata gtgactggga ctgtcaatca agttgctaaa 3840 cagtccggga catgtgacaa cgtgagaaaa caactcagtc aagccaccta cagtgacatt 3900 tgcatggctc cagcagctga tatgtttgag gaaggtgtga aactccaagt tctcaagaag 3960 ggaacaatgt tcccatcaag agctaacaaa ttgtacgaac tcttttgcaa gtacgactct 4020 ttcgattcaa tgcctcctgc tgaacttgaa aggatcgaga agaggatctt caagagggca 4080 cttcaagagg tctgggaaga gaccaaggac ttttacatca atggtttgaa gaatccagag 4140 aagatacaga gagcggaaca tgaccccaaa ctcaagatgt ctttgtgttt cagatggtat 4200 cttggtcttg catcacgttg ggccaacatg ggtgctcctg atcgtgtcat ggactatcaa 4260 gtctggtgtg gtccagccat tggagccttc aatgacttca tcaaaggaac atatcttgat 4320 cctgctgtct ctaatgagta cccatgtgtt gtccagatca accttcagat tttgcgtggt 4380 gcatgttacc tgaggcgtct taatgctctg agaaatgatc cgaggattga tcttgagact 4440 gaagatgctg cctttgtcta tgagccaacg aatgcgcttt ga 4482 <210> 9 <211> 2343 <212> DNA <213> Schizochytrium sp. ATCC_20888 <220> <223> hSzThACS-2 v3 gene <300> <302> POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS <310> US 2007-0245431 A1 <311> 2007-03-15 <312> 2007-10-18 <400> 9 atgcccactc cagatgccac tgcgcctctc aacaagccgt cagactatgc tgtgtaccac 60 gaagaggatg gtcctttctg gactgctgat tcctctggtg tcagcagagt gaacttctct 120 gaaactggtg tgggatctga aggtgtcatt cctgcgctca cactgattga tgtgtttgag 180 cgtgcggtca aacgtggtgg caataggatt gcattcagaa cagagaacat gccaaccttg 240 aggcgtggag aagaggctcc tgatgctttg ccgctcaaag attggaagtc ctggtcttgg 300 aaacagtaca aagctgatgt tcacagaatt gcgagggctc tgatggattt gggagttgag 360 cagcacgatg cggtctccat ctttggcttc aactcaccag aatggttcct cagtgctgtc 420 ggtgcagttc atgctggtgc caagatcgct ggaatctacc cttcagacac tcctgctcaa 480 gtgcagtaca aagccttcca ctctgacact gctgttgcag ttgtggagaa cgaacagtgc 540 ttcaagaaat tcgcagaggt ggttgaggat ctcccttacc tcaaagccat tgtttgttgg 600 gattatgaag ccacagacat cacaagggaa gatggttcag tggttgaagt cctgaccttt 660 gctgagttcc tgaagagagg tgacactgtg gaagcagctg cacttgatga gagaatcagc 720 aagattgagc ccaccatgtg tgctgccctg atctacacca gtggcacaac tgggagaccc 780 aaagctgtca tgatctccca tgacaacctt gtctttgaag catctgctgt ggtcccgaac 840 ttgggagggg catgcacgac cactgctgag gaaaggattc tttcctacct tcctttgtct 900 catgttgctg gaatgatggt tgacatcatt gctccaatca tagcgacagc gttccacaaa 960 ggaaggatct gtgtttgttt tgccagaccg tatgatttga gaactggaac ccttgggcaa 1020 agactcaatg cagtggagcc aaccatattc cttggtgtgc cacgtgtgtg ggaaaagatc 1080 caagagaaat tgatggctgt tggtgccaaa accactggtc tcaagaagaa actcagcacg 1140 gcagccaaga aacgtgggct tgaatttcaa gaggaacaac agatagggag aagtggtgcc 1200 aaccctggct ttggaccgct tggaatctac aagaaacttt tgggcctcat caaaggaaag 1260 ttggggctga ccaaatgcaa gtttgccttt gctggcgcag ctccaatgac ccgtgaaacc 1320 cttcagtact ttggagcgct gaacatcaac atcaatgagg tctatggaat gtctgaatgc 1380 agtggggctg ccacctggtc aactgacaaa gctcatgagt ggggcactgt tggctatgag 1440 atgccctcct gtgaagtcag agtgttcaag attgcggagg atggcaccaa gacagagtgc 1500 ccaagagctg ctgacatcat gcatgccaca gaagaggaac aaggtgaagt ttgcttccgt 1560 ggaaggaaca tcatgatggg atacttggcc aacccgaaac ttggtgatga ccatgttgct 1620 gagattgaag agaagaatgc agctgccata gatagcgaag ggtggcttca ctctggtgac 1680 aagggagcaa tttccacaag gggaatgctc aagataacgg gcagatacaa ggagcttatc 1740 ataggtgctg gtggggagaa tgtggctcct gttcccattg aagatgccat caaggcgagg 1800 atgccttttg tttcaaatgc aatgatggtt ggagacaaga ggaagttcat ggctgtcctt 1860 ctgactttga aaacggttgg agcaactgga gagcttcctg gcacaaacaa gctcatggga 1920 gcagctgcgg actatggtga gaccattgag gatgcctgtg acaatgaggc gctcattgaa 1980 gagataacgc agcaactcaa agagataggt gacgatggag atgtcacgcc aagcaatgca 2040 gcccgtatac aaaagttcac gattttgcca ctcgacttca gtgtctccac agatgaactg 2100 acagcaacgt tgaaattgaa gagaagtgtg gttgcagaca agtatgagga catcatagag 2160 gctttctatg agagcaagtc tgtctttgtg ccttactcaa cagttggagc ttatgcaacg 2220 ggtggaccag ttgatgactc tgttgtggat ggatcattca aaggtgactt tagcatgatt 2280 ggggatgacg atccggatct tcagaatgtg gatgtccttg aagcaattga tgaggacaac 2340 tga 2343 <210> 10 <211> 714 <212> DNA <213> Nostoc sp. PCC 7120 <220> <223> NoHetI v3 gene <300> <308> GenBank: P37695 <309> 1994-10-01 <400> 10 atgcttcagc acacttggct tccgaagcct cccaatctga ccctcttgtc agatgaggtt 60 catctctgga ggattcctct tgaccagcct gagtcacaac ttcaagacct tgctgccacc 120 ctgagcagtg atgaattggc gagggcaaac agattctact ttccagaaca cagaaggcgt 180 ttcactgctg ggagaggcat cctcagatcc atcttgggtg gatacttggg agtggaaccg 240 ggtcaagtca agtttgatta tgagtcccgt gggaaaccga tccttggtga cagatttgct 300 gagagtggac tcctgttcaa cttgtctcac agccagaacc ttgccttgtg tgctgtcaac 360 tacacgcgtc aaataggcat tgatcttgaa tatctgcgtc caacatctga cttggagtct 420 cttgcaaaga ggttctttct cccaagagaa tatgaactct tgaggtcact ccctgatgag 480 cagaaacaga agattttctt tcgttactgg acttgcaaag aggcttatct caaagcaacg 540 ggagatggaa tagccaaact tgaagagatc gagatagcac tcaccccaac agaacctgcc 600 aagctccaaa cagctcctgc gtggtctctg ttggagcttg tgccagatga caattgtgtt 660 gcagctgtgg ctgttgcggg ttttggttgg cagcccaagt tctggcatta ctga 714 <210> 11 <211> 240 <212> DNA <213> Arabidopsis thaliana <220> <223> Chloroplast targeting sequence from ribulose bisphosphate carboxylase small chain 1A <400> 11 atggcttcct ctatgctctc ttccgctact atggttgcct ctccggctca ggccactatg 60 gtcgctcctt tcaacggact taagtcctcc gctgccttcc cagccacccg caaggctaac 120 aacgacatta cttccatcac aagcaacggc ggaagagtta actgcatgca ggtgtggcct 180 ccgattggaa agaagaagtt tgagactctc tcttaccttc ctgaccttac cgattccgaa 240 <210> 12 <211> 29 <212> DNA <213> Artificial sequence <220> <223> TQPATS primer <400> 12 acaagagtgg attgatgatc tagagaggt 29 <210> 13 <211> 29 <212> DNA <213> Artificial sequence <220> <223> TQPATA primer <400> 13 ctttgatgcc tatgtgacac gtaaacagt 29 <210> 14 <211> 29 <212> DNA <213> Artificial sequence <220> <223> TQPATFQ primer <400> 14 ggtgttgtgg ctggtattgc ttacgctgg 29 <210> 15 <211> 23 <212> DNA <213> Artificial sequence <220> <223> HMGF primer <400> 15 cctctctacc accgtctcac atg 23 <210> 16 <211> 20 <212> DNA <213> Artificial sequence <220> <223> HMGR primer <400> 16 gatctggccg gactgtttca 20 <210> 17 <211> 25 <212> DNA <213> Artificial sequence <220> <223> HMG-HEX primer <400> 17 cgctcctcag ctaccacctc aacca 25 <210> 18 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS480 primer <400> 18 cgagttcgga ctcaacatgt tcca 24 <210> 19 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS554 primer <400> 19 aaggttgacg ccagcgacaa cgag 24 <210> 20 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS547 primer <400> 20 aagtttggag ttggcttctg cagc 24 <210> 21 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS581 primer <400> 21 tgagtttggt ctcaacatgt tcca 24 <210> 22 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS556 primer <400> 22 gatgcacgcc aaggtggttg acat 24 <210> 23 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS481 primer <400> 23 taatgtagaa gggcttgtcc tgcg 24 <210> 24 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS550 primer <400> 24 gatgcacgca aaggtggttg acat 24 <210> 25 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS572 primer <400> 25 tgatgtagaa gggtttgtct tgtg 24 <210> 26 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS482 primer <400> 26 catgagatgc atgacgagaa gagg 24 <210> 27 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS483 <400> 27 tggcaacttg gttcactgtt ccag 24 <210> 28 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS488 primer <400> 28 cgcttcgtgt caagaccaac aaga 24 <210> 29 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS489 primer <400> 29 gcaccacgca aaatctgaag gttg 24 <210> 30 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS496 primer <400> 30 gaacttctct gaaactggtg tggg 24 <210> 31 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS497 <400> 31 tcacaggcat cctcaatggt ctca 24 <210> 32 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS500 primer <400> 32 tcagatgagg ttcatctctg gagg 24 <210> 33 <211> 24 <212> DNA <213> Artificial sequence <220> <223> MAS501 primer <400> 33 atctggcaca agctccaaca gaga 24 <210> 34 <211> 2346 <212> DNA <213> Artificial sequence <220> <223> SzACS-2 v4 <400> 34 atggctccca ctcccgacgc caccgcgcct ctgaacaagc cgagcgacta tgccgtctac 60 cacgaggaag acggcccctt ctggaccgcc gattccagcg gcgtctcgcg cgtgaacttt 120 agcgagaccg gcgtgggatc cgagggcgtc atccctgcgc tcacgctcat cgacgtcttc 180 gagagggccg tcaagcgcgg cggaaacagg atcgccttcc gcacggaaaa catgcccacg 240 ctccgccgcg gcgaagaggc cccggacgcg ctgccgctca aggactggaa gtcctggtcc 300 tggaaacagt acaaggccga cgtccaccgc atcgccaggg ctctcatgga cctcggcgtt 360 gagcagcacg acgccgtctc gatttttggc tttaactcgc ccgaatggtt tctcagtgcc 420 gtcggcgccg tgcacgcagg tgccaagatt gccggtattt acccctcaga cacgcccgcc 480 caggtccagt acaaggcctt ccacagtgac accgctgttg ccgttgtcga aaacgagcag 540 tgcttcaaga agttcgccga ggtcgtcgag gaccttcctt acctcaaggc cattgtttgc 600 tgggactatg aagccacaga catcacgcgc gaggacggct ccgtcgtcga ggtcctcacc 660 tttgccgagt tcctcaagcg cggcgacacc gtcgaggcgg ccgcccttga cgagcgcatc 720 tccaagatcg agcccaccat gtgcgctgcc cttatttaca ccagcggtac taccggccgc 780 cccaaggccg ttatgatttc gcacgacaac cttgttttcg aggccagcgc cgtcgtcccc 840 aacctcggag gagcctgtac gaccactgct gaggagcgca ttctctcgta cctgcctctc 900 tcgcacgtcg ctggtatgat ggttgatatt attgccccca tcattgccac cgccttccac 960 aagggccgca tctgcgtctg ctttgctcgc ccgtacgatt tgcgcaccgg cacgctgggc 1020 cagcgcctca acgccgtgga gcccaccatc ttccttggcg tgccccgtgt gtgggaaaag 1080 attcaggaaa agctcatggc cgtcggtgcc aaaaccaccg gcctcaagaa gaagctctct 1140 acggccgcca agaagcgtgg tcttgaattc caggaggagc agcaaatcgg ccgctccggt 1200 gccaaccctg gctttggccc ccttggcatc tacaagaagc tcctcggcct catcaagggc 1260 aagctgggcc tcaccaagtg caagtttgcc tttgctggtg ccgcgcccat gacccgtgag 1320 acccttcagt actttggcgc gctgaacatc aacattaacg aggtctacgg catgtcagag 1380 tgctccggtg ccgccacctg gtccacggac aaggcccacg agtggggcac tgttggctac 1440 gagatgccca gttgcgaggt ccgcgtcttc aagattgccg aggacggtac caagaccgag 1500 tgcccgcgcg ccgccgacat tatgcatgct accgaggagg agcagggcga agtttgcttc 1560 cgcggccgta acatcatgat gggctacctt gccaacccca agcttggcga cgaccacgtt 1620 gccgagatcg aggagaagaa cgctgccgct atcgactccg agggctggct ccacagtggt 1680 gataagggcg ccatttctac ccgcggcatg ctcaagatca cgggccgcta caaggagctc 1740 atcatcggcg ccggtggcga gaacgtggcg cccgtcccta ttgaggacgc catcaaggcg 1800 cgcatgcctt ttgtttccaa cgccatgatg gtcggagata agcgcaagtt catggctgtc 1860 ctccttaccc tcaagacggt tggcgccacg ggcgagcttc ccggtacgaa caagctcatg 1920 ggcgctgccg ccgactacgg tgagaccatc gaggacgcct gcgacaacga ggcgctcatt 1980 gaggagatca cgcagcagct caaggagatc ggtgatgatg gcgatgtcac gccctcgaac 2040 gcggctcgca tccaaaagtt caccattctc ccgctcgact tttccgtctc cacggacgag 2100 ctcacggcca cgctcaagct caagcgctcc gtggtcgcag acaagtacga agacatcatc 2160 gaggcctttt acgagtccaa gagcgttttt gtgccgtact cgaccgttgg cgcctacgcc 2220 acgggcggcc cggtcgacga ctccgttgtc gatggctcat tcaagggcga ctttagcatg 2280 attggcgacg atgatccgga tcttcaaaac gtcgatgtcc tcgaggcgat tgacgaggac 2340 aattaa 2346 <210> 35 <211> 33034 <212> DNA <213> Artificial sequence <220> <223> Plasmid pDAB7361 T-strand <400> 35 cagtcagcat catcacacca aaagttaggc ccgaatagtt tgaaattaga aagctcgcaa 60 ttgaggtcta caggccaaat tcgctcttag ccgtacaata ttactcaccg gatcctaacc 120 ggtttaatta aggcgcgcca tgcccgggca agcggccgca caagtttgta caaaaaagca 180 ggcttctaga cctagctagg tggagtcatc acgcagacta tctcagcatg tgcgtagcac 240 gcggccgcag cttatagtaa agattttttt gttaataaaa tgaaagttaa tatttatgga 300 tttatttgaa catatttaca agtttttgtt aataaattta tttacagaag tgttaaattt 360 gaaaatgtgt taaatgagag ttaatattta tggatataat attactttat ttaaaaataa 420 aatgaattta tattaaaata tgatttggta aaagatatta taaagaggaa cttggatgag 480 aaagacgacg atgggcaaaa gttgcatggc acaccgcttt tgttcagtaa catctgcact 540 gtggcgcctt tttcacactc accatgcatg ctgccacgtc agctttcaca ctatgacacc 600 actggccata cacgttgcca cctcagcttt ctcctcttct cactatgaca cgactggcca 660 tgcatgcatg catgctgcca cctcagctcc cgcctcttca cccgtgtctt tctctcatgt 720 cgcagtgctg cgtgccaacc tcttctctct ataaatagag agaagagaat gatggttaaa 780 ctcaaaccaa aacaagaaaa catacacaaa tagcaaaacg gatccacaac caaaaatggc 840 ggcccgtctg caggagcaaa agggaggcga gatggatacc cgcattgcca tcatcggcat 900 gtcggccatc ctcccctgcg gcacgaccgt gcgcgagtcg tgggagacca tccgcgccgg 960 catcgactgc ctgtcggatc tccccgagga ccgcgtcgac gtgacggcgt actttgaccc 1020 cgtcaagacc accaaggaca agatctactg caagcgcggt ggcttcattc ccgagtacga 1080 ctttgacgcc cgcgagttcg gactcaacat gttccagatg gaggactcgg acgcaaacca 1140 gaccatctcg cttctcaagg tcaaggaggc cctccaggac gccggcatcg acgccctcgg 1200 caaggaaaag aagaacatcg gctgcgtgct cggcattggc ggcggccaaa agtccagcca 1260 cgagttctac tcgcgcctta attatgttgt cgtggagaag gtcctccgca agatgggcat 1320 gcccgaggag gacgtcaagg tcgccgtcga aaagtacaag gccaacttcc ccgagtggcg 1380 cctcgactcc ttccctggct tcctcggcaa cgtcaccgcc ggtcgctgca ccaacacctt 1440 caacctcgac ggcatgaact gcgttgtcga cgccgcatgc gcctcgtccc tcatcgccgt 1500 caaggtcgcc atcgacgagc tgctctacgg tgactgcgac atgatggtca ccggtgccac 1560 ctgcacggat aactccatcg gcatgtacat ggccttctcc aagacccccg tgttctccac 1620 ggaccccagc gtgcgcgcct acgacgaaaa gacaaagggc atgctcatcg gcgagggctc 1680 cgccatgctc gtcctcaagc gctacgccga cgccgtccgc gacggcgatg agatccacgc 1740 tgttattcgc ggctgcgcct cctccagtga tggcaaggcc gccggcatct acacgcccac 1800 catttcgggc caggaggagg ccctccgccg cgcctacaac cgcgcctgtg tcgacccggc 1860 caccgtcact ctcgtcgagg gtcacggcac cggtactccc gttggcgacc gcatcgagct 1920 caccgccttg cgcaacctct ttgacaaggc ctacggcgag ggcaacaccg aaaaggtcgc 1980 tgtgggcagc atcaagtcca gcatcggcca tctcaaggcc gtcgccggtc tcgccggtat 2040 gatcaaggtc atcatggcgc tcaagcacaa gactctcccg ggcaccatca acgtcgacaa 2100 cccacccaac ctctacgaca acacgcccat caacgagtcc tcgctctaca ttaacaccat 2160 gaaccgcccc tggttcccgc cccctggtgt gccccgccgc gccggcattt cgagctttgg 2220 ctttggtggc gccaactacc acgccgtcct cgaggaggcc gagcccgagc acacgaccgc 2280 gtaccgcctc aacaagcgcc cgcagcccgt gctcatgatg gccgccacgc ccgcggccct 2340 ccagtcgctc tgcgaggccc agctcaagga gttcgaggcc gccatcaagg agaacgagac 2400 cgtcaagaac accgcctaca tcaagtgcgt caagttcggc gagcagttca aattccctgg 2460 ctccatcccg gccacaaacg cgcgcctcgg cttcctcgtc aaggatgctg aggatgcctg 2520 ctccaccctc cgtgccatct gcgcccaatt cgccaaggat gtcaccaagg aggcctggcg 2580 cctcccccgc gagggcgtca gcttccgcgc caagggcatc gccaccaacg gcgctgtcgc 2640 cgcgctcttc tccggccagg gcgcgcagta cacgcacatg tttagcgagg tggccatgaa 2700 ctggccccag ttccgccaga gcattgccgc catggacgcc gcccagtcca aggtcgctgg 2760 aagcgacaag gactttgagc gcgtctccca ggtcctctac ccgcgcaagc cgtacgagcg 2820 tgagcccgag caggaccaca agaagatctc cctcaccgcc tactcgcagc cctcgaccct 2880 ggcctgcgct ctcggtgcct ttgagatctt caaggaggcc ggcttcaccc cggactttgc 2940 cgccggccat tcgctcggtg agttcgccgc cctctacgcc gcgggctgcg tcgaccgcga 3000 cgagctcttt gagcttgtct gccgccgcgc ccgcatcatg ggcggcaagg acgcaccggc 3060 cacccccaag ggctgcatgg ccgccgtcat tggccccaac gccgagaaca tcaaggtcca 3120 ggccgccaac gtctggctcg gcaactccaa ctcgccttcg cagaccgtca tcaccggctc 3180 cgtcgaaggt atccaggccg agagcgcccg cctccagaag gagggcttcc gcgtcgtgcc 3240 tcttgcctgc gagagcgcct tccactcgcc ccagatggag aacgcctcgt cggccttcaa 3300 ggacgtcatc tccaaggtct ccttccgcac ccccaaggcc gagaccaagc tcttcagcaa 3360 cgtctctggc gagacctacc ccacggacgc ccgcgagatg cttacgcagc acatgaccag 3420 cagcgtcaag ttcctcaccc aggtccgcaa catgcaccag gccggtgcgc gcatctttgt 3480 cgagttcgga cccaagcagg tgctctccaa gcttgtctcc gagaccctca aggatgaccc 3540 ctcggttgtc accgtctctg tcaacccggc ctcgggcacg gattcggaca tccagctccg 3600 cgacgcggcc gtccagctcg ttgtcgctgg cgtcaacctt cagggctttg acaagtggga 3660 cgcccccgat gccacccgca tgcaggccat caagaagaag cgcactaccc tccgcctttc 3720 ggccgccacc tacgtctcgg acaagaccaa gaaggtccgc gacgccgcca tgaacgatgg 3780 ccgctgcgtc acctacctca agggcgccgc accgctcatc aaggccccgg agcccgttgt 3840 cgacgaggcc gccaagcgcg aggccgagcg tctccagaag gagcttcagg atgcccagcg 3900 ccagctcgac gacgccaagc gcgccgccgc cgaggccaac tccaagctcg ccgctgccaa 3960 ggaggaggcc aagaccgccg ctgcttcggc caagcccgca gttgacactg ctgttgtcga 4020 aaagcatcgt gccatcctca agtccatgct cgcggagctc gatggctacg gatcggtcga 4080 cgcttcttcc ctccagcagc agcagcagca gcagacggcc cccgccccgg tcaaggctgc 4140 tgcgcctgcc gcccccgttg cctcggcccc tgccccggct gtctcgaacg agcttcttga 4200 gaaggccgag actgtcgtca tggaggtcct cgccgccaag accggctacg agaccgacat 4260 gatcgaggct gacatggagc tcgagaccga gctcggcatt gactccatca agcgtgtcga 4320 gatcctctcc gaggtccagg ccatgctcaa tgtcgaggcc aaggatgtcg atgccctcag 4380 ccgcactcgc actgttggtg aggttgtcaa cgccatgaag gccgagatcg ctggcagctc 4440 tgccccggcg cctgctgccg ctgctccggc tccggccaag gctgcccctg ccgccgctgc 4500 gcctgctgtc tcgaacgagc ttctcgagaa ggccgagacc gtcgtcatgg aggtcctcgc 4560 cgccaagact ggctacgaga ctgacatgat cgagtccgac atggagctcg agactgagct 4620 cggcattgac tccatcaagc gtgtcgagat cctctccgag gttcaggcca tgctcaacgt 4680 cgaggccaag gacgtcgacg ctctcagccg cactcgcact gtgggtgagg tcgtcaacgc 4740 catgaaggct gagatcgctg gtggctctgc cccggcgcct gccgccgctg ccccaggtcc 4800 ggctgctgcc gcccctgcgc ctgccgccgc cgcccctgct gtctcgaacg agcttcttga 4860 gaaggccgag accgtcgtca tggaggtcct cgccgccaag actggctacg agactgacat 4920 gatcgagtcc gacatggagc tcgagaccga gctcggcatt gactccatca agcgtgtcga 4980 gattctctcc gaggtccagg ccatgctcaa cgtcgaggcc aaggacgtcg acgctctcag 5040 ccgcacccgc actgttggcg aggtcgtcga tgccatgaag gccgagatcg ctggtggctc 5100 tgccccggcg cctgccgccg ctgctcctgc tccggctgct gccgcccctg cgcctgccgc 5160 ccctgcgcct gctgtctcga gcgagcttct cgagaaggcc gagactgtcg tcatggaggt 5220 cctcgccgcc aagactggct acgagactga catgatcgag tccgacatgg agctcgagac 5280 cgagctcggc attgactcca tcaagcgtgt cgagattctc tccgaggtcc aggccatgct 5340 caacgtcgag gccaaggacg tcgacgctct cagccgcacc cgcactgttg gcgaggtcgt 5400 cgatgccatg aaggccgaga tcgctggtgg ctctgccccg gcgcctgccg ccgctgctcc 5460 tgctccggct gctgccgccc ctgcgcctgc cgcccctgcg cctgccgccc ctgcgcctgc 5520 tgtctcgagc gagcttctcg agaaggccga gactgtcgtc atggaggtcc tcgccgccaa 5580 gactggctac gagactgaca tgattgagtc cgacatggag ctcgagaccg agctcggcat 5640 tgactccatc aagcgtgtcg agattctctc cgaggttcag gccatgctca acgtcgaggc 5700 caaggacgtc gacgctctca gccgcactcg cactgttggt gaggtcgtcg atgccatgaa 5760 ggctgagatc gctggcagct ccgcctcggc gcctgccgcc gctgctcctg ctccggctgc 5820 tgccgctcct gcgcccgctg ccgccgcccc tgctgtctcg aacgagcttc tcgagaaagc 5880 cgagactgtc gtcatggagg tcctcgccgc caagactggc tacgagactg acatgatcga 5940 gtccgacatg gagctcgaga ctgagctcgg cattgactcc atcaagcgtg tcgagatcct 6000 ctccgaggtt caggccatgc tcaacgtcga ggccaaggac gtcgatgccc tcagccgcac 6060 ccgcactgtt ggcgaggttg tcgatgccat gaaggccgag atcgctggtg gctctgcccc 6120 ggcgcctgcc gccgctgccc ctgctccggc tgccgccgcc cctgctgtct cgaacgagct 6180 tctcgagaag gccgagactg tcgtcatgga ggtcctcgcc gccaagactg gctacgagac 6240 cgacatgatc gagtccgaca tggagctcga gaccgagctc ggcattgact ccatcaagcg 6300 tgtcgagatt ctctccgagg ttcaggccat gctcaacgtc gaggccaagg acgtcgatgc 6360 tctcagccgc actcgcactg ttggcgaggt cgtcgatgcc atgaaggctg agatcgccgg 6420 cagctccgcc ccggcgcctg ccgccgctgc tcctgctccg gctgctgccg ctcctgcgcc 6480 cgctgccgct gcccctgctg tctcgagcga gcttctcgag aaggccgaga ccgtcgtcat 6540 ggaggtcctc gccgccaaga ctggctacga gactgacatg attgagtccg acatggagct 6600 cgagactgag ctcggcattg actccatcaa gcgtgtcgag atcctctccg aggttcaggc 6660 catgctcaac gtcgaggcca aggacgtcga tgccctcagc cgcacccgca ctgttggcga 6720 ggttgtcgat gccatgaagg ccgagatcgc tggtggctct gccccggcgc ctgccgccgc 6780 tgcccctgct ccggctgccg ccgcccctgc tgtctcgaac gagcttcttg agaaggccga 6840 gaccgtcgtc atggaggtcc tcgccgccaa gactggctac gagaccgaca tgatcgagtc 6900 cgacatggag ctcgagaccg agctcggcat tgactccatc aagcgtgtcg agattctctc 6960 cgaggttcag gccatgctca acgtcgaggc caaggacgtc gacgctctca gccgcactcg 7020 cactgttggc gaggtcgtcg atgccatgaa ggctgagatc gctggtggct ctgccccggc 7080 gcctgccgcc gctgctcctg cctcggctgg cgccgcgcct gcggtcaaga ttgactcggt 7140 ccacggcgct gactgtgatg atctttccct gatgcacgcc aaggtggttg acatccgccg 7200 cccggacgag ctcatcctgg agcgccccga gaaccgcccc gttctcgttg tcgatgacgg 7260 cagcgagctc accctcgccc tggtccgcgt cctcggcgcc tgcgccgttg tcctgacctt 7320 tgagggtctc cagctcgctc agcgcgctgg tgccgctgcc atccgccacg tgctcgccaa 7380 ggatctttcc gcggagagcg ccgagaaggc catcaaggag gccgagcagc gctttggcgc 7440 tctcggcggc ttcatctcgc agcaggcgga gcgcttcgag cccgccgaaa tcctcggctt 7500 cacgctcatg tgcgccaagt tcgccaaggc ttccctctgc acggctgtgg ctggcggccg 7560 cccggccttt atcggtgtgg cgcgccttga cggccgcctc ggattcactt cgcagggcac 7620 ttctgacgcg ctcaagcgtg cccagcgtgg tgccatcttt ggcctctgca agaccatcgg 7680 cctcgagtgg tccgagtctg acgtcttttc ccgcggcgtg gacattgctc agggcatgca 7740 ccccgaggat gccgccgtgg cgattgtgcg cgagatggcg tgcgctgaca ttcgcattcg 7800 cgaggtcggc attggcgcaa accagcagcg ctgcacgatc cgtgccgcca agctcgagac 7860 cggcaacccg cagcgccaga tcgccaagga cgacgtgctg ctcgtttctg gcggcgctcg 7920 cggcatcacg cctctttgca tccgggagat cacgcgccag atcgcgggcg gcaagtacat 7980 tctgcttggc cgcagcaagg tctctgcgag cgaaccggca tggtgcgctg gcatcactga 8040 cgagaaggct gtgcaaaagg ctgctaccca ggagctcaag cgcgccttta gcgctggcga 8100 gggccccaag cccacgcccc gcgctgtcac taagcttgtg ggctctgttc ttggcgctcg 8160 cgaggtgcgc agctctattg ctgcgattga agcgctcggc ggcaaggcca tctactcgtc 8220 gtgcgacgtg aactctgccg ccgacgtggc caaggccgtg cgcgatgccg agtcccagct 8280 cggtgcccgc gtctcgggca tcgttcatgc ctcgggcgtg ctccgcgacc gtctcatcga 8340 gaagaagctc cccgacgagt tcgacgccgt ctttggcacc aaggtcaccg gtctcgagaa 8400 cctcctcgcc gccgtcgacc gcgccaacct caagcacatg gtcctcttca gctcgctcgc 8460 cggcttccac ggcaacgtcg gccagtctga ctacgccatg gccaacgagg cccttaacaa 8520 gatgggcctc gagctcgcca aggacgtctc ggtcaagtcg atctgcttcg gtccctggga 8580 cggtggcatg gtgacgccgc agctcaagaa gcagttccag gagatgggcg tgcagatcat 8640 cccccgcgag ggcggcgctg ataccgtggc gcgcatcgtg ctcggctcct cgccggctga 8700 gatccttgtc ggcaactggc gcaccccgtc caagaaggtc ggctcggaca ccatcaccct 8760 gcaccgcaag atttccgcca agtccaaccc cttcctcgag gaccacgtca tccagggccg 8820 ccgcgtgctg cccatgacgc tggccattgg ctcgctcgcg gagacctgcc tcggcctctt 8880 ccccggctac tcgctctggg ccattgacga cgcccagctc ttcaagggtg tcactgtcga 8940 cggcgacgtc aactgcgagg tgaccctcac cccgtcgacg gcgccctcgg gccgcgtcaa 9000 cgtccaggcc acgctcaaga ccttttccag cggcaagctg gtcccggcct accgcgccgt 9060 catcgtgctc tccaaccagg gcgcgccccc ggccaacgcc accatgcagc cgccctcgct 9120 cgatgccgat ccggcgctcc agggctccgt ctacgacggc aagaccctct tccacggccc 9180 ggccttccgc ggcatcgatg acgtgctctc gtgcaccaag agccagcttg tggccaagtg 9240 cagcgctgtc cccggctccg acgccgctcg cggcgagttt gccacggaca ctgacgccca 9300 tgaccccttc gtgaacgacc tggcctttca ggccatgctc gtctgggtgc gccgcacgct 9360 cggccaggct gcgctcccca actcgatcca gcgcatcgtc cagcaccgcc cggtcccgca 9420 ggacaagccc ttctacatta ccctccgctc caaccagtcg ggcggtcact cccagcacaa 9480 gcacgccctt cagttccaca acgagcaggg cgatctcttc attgatgtcc aggcttcggt 9540 catcgccacg gacagccttg ccttctaaat ctaaactagg ccatggtgac ttaaggtagt 9600 tagcttaatc acttaggtca cccagtccaa gcccggccta gcacgatgat catgagtgtt 9660 gctattgatg tatgttaata ctatacacag tcatcgtgtg tgttttataa aactgtaagc 9720 ttaggatgtt tgaggctaat gtaattagca ctacaccgta ataaatgaga ggtttacttt 9780 atgttttaat aatgttctta cccttgctgc tctctaaact gtaatggcgt acggttagct 9840 aaactaaagt aaaaatcgat tcgcttgagt aaaattagtg accgggaaag atcatcgact 9900 actactgctc agagatgtaa tcggtaatta agacttcaag ctcaataatt aattaggagt 9960 aaatctggct tcgaattttt aaattgtcaa cttccaaaga aggattggca agactcaatc 10020 tcaattgccc aacaaacata tggctcttta caaatttgga atctcttttt gatggcaaga 10080 acgtcgtaat tcctctgtcg atctcatgta attaccaaac tcatctactt attactttct 10140 ttatatgcac ttactgatgc ggccgcttaa ttaagatgag tgatactcag gactcaggac 10200 tcactctgct gatcactagt gctagcctcg aggtcgacac aactttgtat acaaaagttg 10260 tctagaccta ggctcagcaa ctcatccagt caagttcgag tcagtgagtt gggcggccgc 10320 agcttatagt aaagattttt ttgttaataa aatgaaagtt aatatttatg gatttatttg 10380 aacatattta caagtttttg ttaataaatt tatttacaga agtgttaaat ttgaaaatgt 10440 gttaaatgag agttaatatt tatggatata atattacttt atttaaaaat aaaatgaatt 10500 tatattaaaa tatgatttgg taaaagatat tataaagagg aacttggatg agaaagacga 10560 cgatgggcaa aagttgcatg gcacaccgct tttgttcagt aacatctgca ctgtggcgcc 10620 tttttcacac tcaccatgca tgctgccacg tcagctttca cactatgaca ccactggcca 10680 tacacgttgc cacctcagct ttctcctctt ctcactatga cacgactggc catgcatgca 10740 tgcatgctgc cacctcagct cccgcctctt cacccgtgtc tttctctcat gtcgcagtgc 10800 tgcgtgccaa cctcttctct ctataaatag agagaagaga atgatggtta aactcaaacc 10860 aaaacaagaa aacatacaca aatagcaaaa cggatccaca accaaaaatg gctgcaagga 10920 acgtgagtgc ggctcatgag atgcatgacg agaagaggat agctgttgtg ggcatggctg 10980 ttcagtatgc tggatgcaaa acgaaagacg agttctggga agttctcatg aatggaaaag 11040 ttgagagcaa agtgatcagc gacaaacgtc ttggttcaaa ttaccgtgct gaacactaca 11100 aagctgaaag gagcaagtat gctgacacct tctgcaatga gacatatgga actcttgatg 11160 agaacgaaat agacaatgag catgaactcc ttttgaacct tgccaaacaa gccttggctg 11220 aaacatctgt caaagattca acaagatgtg gcatagtttc tggatgcctc agcttcccga 11280 tggacaacct ccaaggtgaa ctcttgaatg tgtaccagaa ccatgttgag aagaaacttg 11340 gtgccagagt tttcaaagat gcctctcact ggtcagaaag agaacagtcc aacaaaccag 11400 aggctggtga cagaagaatc ttcatggacc ctgcttcctt tgttgctgag gaactcaatc 11460 ttggcgctct ccactactct gttgatgctg cctgtgccac tgcgctctat gtgctcagac 11520 tggcgcaaga tcacttggtc tctggtgcag ctgatgtgat gctttgtggt gcaacatgcc 11580 tgccggagcc gtttttcata ctctctgggt tctcaacctt ccaagccatg cctgttggaa 11640 cgggtcagaa tgtgtccatg ccgctgcaca aagattctca aggattgacc cctggtgaag 11700 gaggttccat catggtcctc aaacgtttgg atgacgcaat cagagatgga gatcacatct 11760 atggaacact tttgggagcc aatgtcagca acagtggcac tggtctgcca cttaaacccc 11820 ttttgccgtc agagaaaaag tgcctcatgg acacctacac acgtatcaat gtgcatcccc 11880 acaagattca gtatgttgaa tgccatgcaa ctgggactcc acaaggtgat cgtgtggaaa 11940 ttgatgctgt gaaggcttgc tttgaaggca aagtcccacg ttttggaacc acaaaaggga 12000 actttggaca cacattggtg gcagctgggt ttgctggcat gtgcaaagtg cttctctcca 12060 tgaagcacgg gatcattcca cccactccgg gaatagatga cgaaacgaag atggaccctc 12120 tggttgtctc tggtgaggcc attccctggc cagaaacgaa tggagaaccg aagcgtgctg 12180 gtctctcagc gtttggattt ggtgggacca atgctcatgc tgtctttgag gaacatgacc 12240 caagcaatgc tgcatgcact ggccatgact ccatttctgc tctctctgca cgttgtggtg 12300 gtgagtcaaa catgagaata gcaatcactg ggatggatgc cacttttggt gctttgaaag 12360 gattggatgc ttttgaaagg gccatctaca ctggtgctca tggtgctata ccactcccag 12420 aaaagagatg gagattcctg gggaaagaca aggacttcct tgatctttgt ggagtcaaag 12480 caacaccgca tggctgttac attgaagatg ttgaagttga tttccagagg ctgaggactc 12540 caatgactcc tgaagatatg ctcttgcctc aacagcttct ggctgtcacc acaattgata 12600 gggccatatt ggactctggc atgaagaaag gtggaaatgt ggctgtcttt gtggggttgg 12660 ggaccgatct tgaactctac cgtcaccgtg caagggttgc tctcaaggag agagtgaggc 12720 ctgaagccag caagaaactc aatgacatga tgcagtacat caatgattgt gggacaagca 12780 cttcttacac cagctacata ggcaacttgg ttgcaacaag ggtcagttca caatggggct 12840 tcacgggacc ttccttcaca atcacagaag gcaacaattc tgtgtacaga tgtgctgaac 12900 ttggcaaata cctcttggaa acgggagagg ttgatggggt tgtggttgcg ggtgttgatc 12960 tctgtgggag tgctgagaac ctttacgtca agtcaaggcg tttcaaggtg tctacatctg 13020 acaccccgag agccagcttt gatgcagctg ctgatggtta ctttgttgga gagggctgtg 13080 gtgcatttgt gctcaagcgt gagacaagct gcaccaagga tgacagaatc tatgcttgca 13140 tggatgccat agttcctgga aatgttccat ctgcatgttt gagagaggca ttggatcaag 13200 ccagagtcaa gcctggagac atcgagatgt tggagctttc tgctgattca gcgaggcatt 13260 tgaaggaccc gtctgttctc ccaaaggaac tcactgctga ggaagagatt ggcggacttc 13320 agacgattct tcgtgacgat gacaagctcc caaggaatgt tgcaactgga agtgtcaagg 13380 ccactgttgg tgacactggt tatgcttctg gtgctgccag tttgattaag gctgcgcttt 13440 gcatctacaa ccgttacctg cccagcaatg gagatgactg ggatgaacct gcacctgaag 13500 caccctggga cagcacactc tttgcgtgcc agacctcaag agcttggttg aagaaccctg 13560 gtgagagacg ttatgcggct gtttccggag tcagtgaaac cagaagttgc tacagtgtgc 13620 tgctttctga agcagaggga cactacgaga gagagaacag aatcagtttg gatgaggaag 13680 cacccaagct cattgtgttg agagcagact cccatgaaga gattcttgga agattggaca 13740 agataaggga aaggttcttg cagcccacgg gtgctgcacc cagagagtct gaattgaagg 13800 cgcaagcccg taggatcttt cttgaactcc ttggtgagac ccttgcccaa gatgcagctt 13860 catctggaag tcagaagccc cttgctctca gcttggtctc cactccatcc aagctccaga 13920 gagaggtgga acttgcggca aaaggcattc cgagatgtct caagatgaga cgtgattgga 13980 gttcccctgc tggaagcaga tatgcgcctg agcctttggc cagtgacaga gttgccttca 14040 tgtatggaga aggaaggagc ccttactatg gcatcacaca agacattcat aggatttggc 14100 ctgaacttca tgaggtcatc aatgagaaaa caaaccgtct ctgggctgaa ggtgacagat 14160 gggtcatgcc aagggccagc ttcaagagtg agcttgagtc acaacagcaa gagtttgaca 14220 gaaacatgat tgaaatgttc cgtcttggaa tcctcacctc aattgccttc acaaacctgg 14280 cgagagatgt tctcaacatc acgcccaaag cagcctttgg actcagtttg ggagagattt 14340 ccatgatctt tgctttctcc aagaaaaacg gtttgataag cgatcagttg acaaaggatt 14400 tgagagagtc agatgtctgg aacaaagctc tggctgttga gttcaatgcg ctcagagaag 14460 cctggggcat tccacagtct gtcccaaaag atgagttctg gcaaggttac atagtgaggg 14520 gaacgaaaca agacatagaa gcagccatag caccagactc aaagtatgtg aggttgacca 14580 tcatcaatga tgccaacact gctctcatca gtggaaagcc tgatgcatgc aaagctgcga 14640 ttgctcgtct tggtggcaac atccctgcgc ttccagtcac tcaaggcatg tgtgggcatt 14700 gcccagaggt gggaccttac accaaggaca tcgcaaagat ccacgccaac cttgagttcc 14760 ctgtggttga tggtcttgat ctctggacta ccataaacca gaagaggctt gtgccaagag 14820 ccacgggtgc caaagatgaa tgggctcctt catcctttgg ggagtatgct ggacagctct 14880 atgagaaaca agccaacttc cctcagatag ttgaaaccat ctacaagcag aactatgatg 14940 tgtttgttga agttgggcca aacaatcaca gaagcacagc agtgagaacc acgcttggac 15000 cacagagaaa ccaccttgct ggtgccatag acaagcagaa cgaggatgct tggacgacaa 15060 tagtcaagct cgtggcttca ttgaaggctc accttgttcc tggagtgacc atcagtccgc 15120 tctaccactc caagctcgtt gcagaggctg aggcttgtta tgctgcgctc tgtaaaggtg 15180 agaaacccaa gaaaaacaag tttgtgagaa agattcagtt gaatggcaga ttcaacagca 15240 aggcagaccc catctcctca gcagaccttg catccttccc acctgcggac cctgcgattg 15300 aagctgccat ctcttcaagg atcatgaagc ctgtggctcc gaaattctat gcgcgtctca 15360 acattgatga gcaagatgag accagagatc cgatcttgaa caaggacaat gcgccttcca 15420 gctccagctc ttccagcagt tcctcttcca gcagttcatc tccgtctcct gctccttcag 15480 ctccagttca gaagaaagct gccccagctg cggagacaaa agctgttgcc agtgctgatg 15540 cacttcgtag tgccctgctt gatcttgact ccatgcttgc gctcagctct gcaagtgctt 15600 ctggcaacct tgttgagact gcgccatctg atgccagtgt cattgtccct ccgtgcaaca 15660 ttgctgatct tggcagcaga gctttcatga aaacttatgg tgtttctgcg cctctgtaca 15720 ccggtgcaat ggccaaggga attgcctctg cggacttggt gatcgcagct ggaaggcaag 15780 gaatccttgc gtcatttggt gctggaggcc ttccaatgca agttgtgcgt gagtccattg 15840 aaaagattca agctgccctg cccaatggac catatgctgt caacctcatc cattctcctt 15900 ttgacagcaa ccttgagaag ggaaatgttg atctcttctt ggaaaagggt gtcacttttg 15960 ttgaggcttc agccttcatg acgttgacac cgcaagttgt cagatacaga gcggctgggt 16020 tgacgaggaa tgcagatggg tcagtcaaca taaggaacag aatcattggg aaagtctcca 16080 gaactgagct ggctgaaatg ttcatgcgtc ctgcacctga gcatcttttg cagaagctca 16140 ttgcttctgg ggagatcaat caagaacaag ctgagttggc cagacgtgtt cctgtggctg 16200 atgacattgc ggttgaagct gattctggtg gacacactga caaccgtcca atccatgtca 16260 ttctgcctct gattatcaac ctgagggata ggcttcacag agagtgtgga tacccagcaa 16320 acctgagggt tcgtgttggt gctgggggtg gcattgggtg tccccaagct gcgctggcaa 16380 ctttcaacat gggtgcctct ttcattgtca ctggaacagt gaaccaagtt gccaaacaat 16440 ctgggacgtg tgacaatgtg aggaaacagc ttgcgaaagc aacttactca gatgtttgca 16500 tggcaccggc agctgacatg tttgaggaag gtgtgaaact tcaagttttg aagaaaggaa 16560 caatgttccc ttcaagagcc aacaaactct atgagttgtt ctgcaagtat gattcttttg 16620 agtccatgcc tccagcagag cttgcgaggg ttgagaaaag aatcttcagt cgtgcattgg 16680 aagaggtctg ggatgagacc aagaacttct acatcaaccg tctccacaac ccagagaaga 16740 tacagagggc tgagagggac cccaaactga agatgtcact gtgtttcaga tggtatctgt 16800 ccctggcttc aaggtgggcc aacactggag cttctgacag agtcatggac taccaagttt 16860 ggtgtggtcc tgccattggt tcattcaatg atttcatcaa aggaacttac cttgatccgg 16920 cagtggcaaa tgagtacccg tgtgttgtgc agatcaacaa acagattctt agaggagcgt 16980 gcttcttgag gcgtcttgaa atactgagaa atgcaaggct ttcagatggt gcagctgcac 17040 ttgtggcgag cattgatgac acatatgtcc ctgctgagaa gctgtgagta gttagcttaa 17100 tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga 17160 tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg 17220 tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta 17280 ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa 17340 gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc 17400 tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg 17460 cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc 17520 ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta 17580 attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc 17640 acttactgat gcggccgctt aattaatctg acctgaccac tagacctgag tgagtacaga 17700 tgtgctacta gtgctagcct cgaggtcgac caacttttct atacaaagtt gtctagacct 17760 aggactaggc actgagtgac tcactgacct gactgagtga ctggcggccg cagcttatag 17820 taaagatttt tttgttaata aaatgaaagt taatatttat ggatttattt gaacatattt 17880 acaagttttt gttaataaat ttatttacag aagtgttaaa tttgaaaatg tgttaaatga 17940 gagttaatat ttatggatat aatattactt tatttaaaaa taaaatgaat ttatattaaa 18000 atatgatttg gtaaaagata ttataaagag gaacttggat gagaaagacg acgatgggca 18060 aaagttgcat ggcacaccgc ttttgttcag taacatctgc actgtggcgc ctttttcaca 18120 ctcaccatgc atgctgccac gtcagctttc acactatgac accactggcc atacacgttg 18180 ccacctcagc tttctcctct tctcactatg acacgactgg ccatgcatgc atgcatgctg 18240 ccacctcagc tcccgcctct tcacccgtgt ctttctctca tgtcgcagtg ctgcgtgcca 18300 acctcttctc tctataaata gagagaagag aatgatggtt aaactcaaac caaaacaaga 18360 aaacatacac aaatagcaaa acggatccac aaccaaaaat ggcgcttcgt gtcaagacca 18420 acaagaagcc atgttgggag atgaccaagg aggaactgac ctctggcaaa actgaggtgt 18480 tcaactatga ggaactcctg gagtttgcag agggtgacat agctaaggtt tttggtccgg 18540 agtttgctgt catagacaag taccccagaa gggtgaggct gcctgctcgt gaatacctgc 18600 ttgtgacaag ggtcacactc atggatgccg aggtcaacaa ctacagagtt ggtgccagaa 18660 tggtcactga gtatgatctt ccagtgaatg gcgaattgtc tgagggaggt gattgtccct 18720 gggcagttct ggttgagagt ggacagtgcg atttgatgct gatatcatac atgggaattg 18780 atttccagaa ccaaggtgac agagtttacc gtctgctcaa caccacgttg acattctatg 18840 gggttgccca tgagggagag acccttgagt atgacatcag agtgaccggt tttgccaaac 18900 gtttggatgg tggcatctcc atgttcttct ttgagtatga ctgctatgtc aatgggaggc 18960 ttctcattga gatgagagat ggctgtgctg gcttcttcac aaatgaggag cttgatgcgg 19020 gaaagggtgt ggtgttcaca agaggggatt tggctgccag agctaagatt ccaaagcaag 19080 atgtttctcc ctatgctgtc gctccctgcc ttcacaaaac aaaactcaac gaaaaggaga 19140 tgcagacctt ggttgacaaa gattgggctt ccgtttttgg ctcaaagaat ggcatgccgg 19200 agatcaacta caaactctgc gcacgtaaga tgctcatgat tgatagagtc accagcattg 19260 accataaggg aggtgtgtac ggtttgggtc agttggttgg tgagaaaatc ttggagaggg 19320 accattggta cttcccatgc cattttgtta aggatcaagt catggctgga tctcttgtca 19380 gcgacggttg ttcacaaatg ctcaagatgt acatgatctg gctcggtttg caccttacca 19440 ccggaccatt tgacttcaga cccgtcaacg gacacccgaa caaagttagg tgtaggggtc 19500 agatatctcc tcataaggga aagttggtct acgtcatgga gatcaaagag atgggattcg 19560 acgaggataa cgatccgtac gccattgctg acgtcaacat cattgacgtt gatttcgaaa 19620 agggtcaaga tttcagcctt gatagaatca gcgactacgg aaagggggat cttaacaaaa 19680 agatcgtcgt ggacttcaag ggcattgctc tcaagatgca gaagagatcc acgaacaaga 19740 acccctccaa agttcagccc gtttttgcta acggtgcagc cactgtggga ccggaagcgt 19800 ccaaggccag ctctggtgcc agtgctagtg cttctgctgc ccctgcgaag cctgctttct 19860 cagcagatgt tcttgcgcca aagccagttg cacttcctga acatatcctc aagggtgacg 19920 ctctcgctcc gaaagaaatg tcctggcatc ccatgtcaaa acttcctggc aacccgacgc 19980 cgttcttctc ccctagttct taccctccga gggcaatttg cttcatccct ttccctggca 20040 acccacttga caacaattgc aaagctggag aaatgcctct gaactggtac aacatgtcag 20100 agttcatgtg tggaaaagtt agcaactgct tgggaccaga gttcgcaaga tttgataagt 20160 caaatacgag cagaagccct gcttttgatt tggctcttgt gactagagtt gttgaagtca 20220 caaacatgga acatggcaag ttcctgaatg ttgattgcaa tcccagcaaa ggcacaatgg 20280 tgggggagtt tgactgtcct caagatgcgt ggttctttga tggttcttgc aatgatgggc 20340 acatgcctta ttccatcatc atggaaatcg gacttcagac atccggtgtt ctcaccagtg 20400 tgttgaaagc accactgacg atggacaaag atgacattct ctttaggaac cttgatgcaa 20460 gtgctgaaat ggtgaggcca gatgtggatg tgagggggaa aaccataagg aatgtgacca 20520 agtgcactgg atatgcaatg ttgggaaaga tggggattca cagattcacg tttgagttgt 20580 ctgttgatgg cgtggttttc tacaaaggaa gcacttcctt tggatggttc actccagagg 20640 tgtttgctca gcaagctgga cttgacaatg ggaaaaagac ggagccttgg tgcaaaacta 20700 acaacacgag tgtgagaaga gttgaaatcg catcagccaa aggcaaagaa cagctgactg 20760 agaaacttcc agatgcaact aatgctcaag ttctcagaag gtcagaacag tgtgagtatt 20820 tggattacct caacattgct cctgattctg ggctgcatgg gaaaggatat gcgcatggac 20880 acaaagatgt taacccacaa gactggttct tctcttgtca cttttggttt gatcctgtga 20940 tgcctggatc tttgggaatt gagtcaatgt tccagctcat tgaggcattt gcggtggacc 21000 agaacatacc gggagaatac aatgtgagca atccgacctt tgcccatgcc cctggaaaaa 21060 cggcgtggaa gtaccgtggt caattgacac caaagaaccg tgcgatggac tgtgaggtgc 21120 acatagtttc aatcacagcc tctcctgaaa atggaggcta cgttgacata gtggcagatg 21180 gagcactttg ggttgatggt ctccgtgtgt atgaagccaa agaactcaga gttcgtgttg 21240 tcagtgcaaa acctcaagcc attccggatg ttcaacaaca gccacccagc gcaaaagcgg 21300 accctgggaa aactggagtt gcactcagtc cgactcagtt gagagatgtc ctgcttgaag 21360 tggacaatcc attgtatctt ggtgttgaaa actccaattt ggtgcagttt gaatccaaac 21420 ctgcaacttc ttcaaggatt gtcagtatca aaccatgctc cataagtgat cttggtgaca 21480 agtccttcat ggaaacgtac aatgtgtcag cacctctgta cactggagca atggccaagg 21540 gaattgcgtc tgcggacctt gtcattgctg ctgggaagag gaagatattg ggttcttttg 21600 gagcgggtgg gcttccaatg caccatgtga gggcagcttt ggagaagata caagcggctc 21660 tgcctcaagg accatatgct gtgaacctca ttcattcacc ttttgacagc aacttggaaa 21720 aggggaatgt tgatctcttc ctcgaaaagg gcgttactgt ggtggaggct tctgcattca 21780 tgaccctcac tccccaagtt gttcgttacc gtgcagccgg actctcaagg aacgctgatg 21840 ggagtgtcaa catcagaaac cgtatcattg gaaaagtgtc cagaacagaa ctcgccgaaa 21900 tgttcatcag accggctccg gaacacctcc ttgagaaact catagcttct ggtgagataa 21960 cacaagaaca agccgaactt gcccgtcgtg ttcctgtcgc agacgacatc gctgttgaag 22020 ctgattctgg tggtcacact gacaaccgtc ccatccacgt catcttgcct ttgatcatca 22080 accttaggaa cagactgcac agagagtgtg gttatcctgc gcaccttaga gtcagagttg 22140 gagctggagg cggtgttggc tgcccacaag ctgctgcggc tgccctcaca atgggagctg 22200 ccttcatagt gactgggact gtcaatcaag ttgctaaaca gtccgggaca tgtgacaacg 22260 tgagaaaaca actcagtcaa gccacctaca gtgacatttg catggctcca gcagctgata 22320 tgtttgagga aggtgtgaaa ctccaagttc tcaagaaggg aacaatgttc ccatcaagag 22380 ctaacaaatt gtacgaactc ttttgcaagt acgactcttt cgattcaatg cctcctgctg 22440 aacttgaaag gatcgagaag aggatcttca agagggcact tcaagaggtc tgggaagaga 22500 ccaaggactt ttacatcaat ggtttgaaga atccagagaa gatacagaga gcggaacatg 22560 accccaaact caagatgtct ttgtgtttca gatggtatct tggtcttgca tcacgttggg 22620 ccaacatggg tgctcctgat cgtgtcatgg actatcaagt ctggtgtggt ccagccattg 22680 gagccttcaa tgacttcatc aaaggaacat atcttgatcc tgctgtctct aatgagtacc 22740 catgtgttgt ccagatcaac cttcagattt tgcgtggtgc atgttacctg aggcgtctta 22800 atgctctgag aaatgatccg aggattgatc ttgagactga agatgctgcc tttgtctatg 22860 agccaacgaa tgcgctttga gtagttagct taatcactta ggtcacccag tccaagcccg 22920 gcctagcacg atgatcatga gtgttgctat tgatgtatgt taatactata cacagtcatc 22980 gtgtgtgttt tataaaactg taagcttagg atgtttgagg ctaatgtaat tagcactaca 23040 ccgtaataaa tgagaggttt actttatgtt ttaataatgt tcttaccctt gctgctctct 23100 aaactgtaat ggcgtacggt tagctaaact aaagtaaaaa tcgattcgct tgagtaaaat 23160 tagtgaccgg gaaagatcat cgactactac tgctcagaga tgtaatcggt aattaagact 23220 tcaagctcaa taattaatta ggagtaaatc tggcttcgaa tttttaaatt gtcaacttcc 23280 aaagaaggat tggcaagact caatctcaat tgcccaacaa acatatggct ctttacaaat 23340 ttggaatctc tttttgatgg caagaacgtc gtaattcctc tgtcgatctc atgtaattac 23400 caaactcatc tacttattac tttctttata tgcacttact gatgcggccg cttaattaat 23460 cacgagtgga tgactgtcac gactgactca tgactgacta ctagtgctag cctcgaggtc 23520 gacacaactt tgtataataa agttgtctag acctaggcga gtcatgagtc gtgacatgac 23580 gtcatgctca tcagactgcg gccgcagctt atagtaaaga tttttttgtt aataaaatga 23640 aagttaatat ttatggattt atttgaacat atttacaagt ttttgttaat aaatttattt 23700 acagaagtgt taaatttgaa aatgtgttaa atgagagtta atatttatgg atataatatt 23760 actttattta aaaataaaat gaatttatat taaaatatga tttggtaaaa gatattataa 23820 agaggaactt ggatgagaaa gacgacgatg ggcaaaagtt gcatggcaca ccgcttttgt 23880 tcagtaacat ctgcactgtg gcgccttttt cacactcacc atgcatgctg ccacgtcagc 23940 tttcacacta tgacaccact ggccatacac gttgccacct cagctttctc ctcttctcac 24000 tatgacacga ctggccatgc atgcatgcat gctgccacct cagctcccgc ctcttcaccc 24060 gtgtctttct ctcatgtcgc agtgctgcgt gccaacctct tctctctata aatagagaga 24120 agagaatgat ggttaaactc aaaccaaaac aagaaaacat acacaaatag caaaacggat 24180 ccacaaccaa aaatgcccac tccagatgcc actgcgcctc tcaacaagcc gtcagactat 24240 gctgtgtacc acgaagagga tggtcctttc tggactgctg attcctctgg tgtcagcaga 24300 gtgaacttct ctgaaactgg tgtgggatct gaaggtgtca ttcctgcgct cacactgatt 24360 gatgtgtttg agcgtgcggt caaacgtggt ggcaatagga ttgcattcag aacagagaac 24420 atgccaacct tgaggcgtgg agaagaggct cctgatgctt tgccgctcaa agattggaag 24480 tcctggtctt ggaaacagta caaagctgat gttcacagaa ttgcgagggc tctgatggat 24540 ttgggagttg agcagcacga tgcggtctcc atctttggct tcaactcacc agaatggttc 24600 ctcagtgctg tcggtgcagt tcatgctggt gccaagatcg ctggaatcta cccttcagac 24660 actcctgctc aagtgcagta caaagccttc cactctgaca ctgctgttgc agttgtggag 24720 aacgaacagt gcttcaagaa attcgcagag gtggttgagg atctccctta cctcaaagcc 24780 attgtttgtt gggattatga agccacagac atcacaaggg aagatggttc agtggttgaa 24840 gtcctgacct ttgctgagtt cctgaagaga ggtgacactg tggaagcagc tgcacttgat 24900 gagagaatca gcaagattga gcccaccatg tgtgctgccc tgatctacac cagtggcaca 24960 actgggagac ccaaagctgt catgatctcc catgacaacc ttgtctttga agcatctgct 25020 gtggtcccga acttgggagg ggcatgcacg accactgctg aggaaaggat tctttcctac 25080 cttcctttgt ctcatgttgc tggaatgatg gttgacatca ttgctccaat catagcgaca 25140 gcgttccaca aaggaaggat ctgtgtttgt tttgccagac cgtatgattt gagaactgga 25200 acccttgggc aaagactcaa tgcagtggag ccaaccatat tccttggtgt gccacgtgtg 25260 tgggaaaaga tccaagagaa attgatggct gttggtgcca aaaccactgg tctcaagaag 25320 aaactcagca cggcagccaa gaaacgtggg cttgaatttc aagaggaaca acagataggg 25380 agaagtggtg ccaaccctgg ctttggaccg cttggaatct acaagaaact tttgggcctc 25440 atcaaaggaa agttggggct gaccaaatgc aagtttgcct ttgctggcgc agctccaatg 25500 acccgtgaaa cccttcagta ctttggagcg ctgaacatca acatcaatga ggtctatgga 25560 atgtctgaat gcagtggggc tgccacctgg tcaactgaca aagctcatga gtggggcact 25620 gttggctatg agatgccctc ctgtgaagtc agagtgttca agattgcgga ggatggcacc 25680 aagacagagt gcccaagagc tgctgacatc atgcatgcca cagaagagga acaaggtgaa 25740 gtttgcttcc gtggaaggaa catcatgatg ggatacttgg ccaacccgaa acttggtgat 25800 gaccatgttg ctgagattga agagaagaat gcagctgcca tagatagcga agggtggctt 25860 cactctggtg acaagggagc aatttccaca aggggaatgc tcaagataac gggcagatac 25920 aaggagctta tcataggtgc tggtggggag aatgtggctc ctgttcccat tgaagatgcc 25980 atcaaggcga ggatgccttt tgtttcaaat gcaatgatgg ttggagacaa gaggaagttc 26040 atggctgtcc ttctgacttt gaaaacggtt ggagcaactg gagagcttcc tggcacaaac 26100 aagctcatgg gagcagctgc ggactatggt gagaccattg aggatgcctg tgacaatgag 26160 gcgctcattg aagagataac gcagcaactc aaagagatag gtgacgatgg agatgtcacg 26220 ccaagcaatg cagcccgtat acaaaagttc acgattttgc cactcgactt cagtgtctcc 26280 acagatgaac tgacagcaac gttgaaattg aagagaagtg tggttgcaga caagtatgag 26340 gacatcatag aggctttcta tgagagcaag tctgtctttg tgccttactc aacagttgga 26400 gcttatgcaa cgggtggacc agttgatgac tctgttgtgg atggatcatt caaaggtgac 26460 tttagcatga ttggggatga cgatccggat cttcagaatg tggatgtcct tgaagcaatt 26520 gatgaggaca actgagtagt tagcttaatc acttaggtca cccagtccaa gcccggccta 26580 gcacgatgat catgagtgtt gctattgatg tatgttaata ctatacacag tcatcgtgtg 26640 tgttttataa aactgtaagc ttaggatgtt tgaggctaat gtaattagca ctacaccgta 26700 ataaatgaga ggtttacttt atgttttaat aatgttctta cccttgctgc tctctaaact 26760 gtaatggcgt acggttagct aaactaaagt aaaaatcgat tcgcttgagt aaaattagtg 26820 accgggaaag atcatcgact actactgctc agagatgtaa tcggtaatta agacttcaag 26880 ctcaataatt aattaggagt aaatctggct tcgaattttt aaattgtcaa cttccaaaga 26940 aggattggca agactcaatc tcaattgccc aacaaacata tggctcttta caaatttgga 27000 atctcttttt gatggcaaga acgtcgtaat tcctctgtcg atctcatgta attaccaaac 27060 tcatctactt attactttct ttatatgcac ttactgatgc ggccgcttaa ttaatctgat 27120 catggatgct acgtcacggc agtacaggac tatcactaga cctaggagct gtgctgggta 27180 actcacatga gtcactggtc acatcagcgg ccgcagctta tagtaaagat ttttttgtta 27240 ataaaatgaa agttaatatt tatggattta tttgaacata tttacaagtt tttgttaata 27300 aatttattta cagaagtgtt aaatttgaaa atgtgttaaa tgagagttaa tatttatgga 27360 tataatatta ctttatttaa aaataaaatg aatttatatt aaaatatgat ttggtaaaag 27420 atattataaa gaggaacttg gatgagaaag acgacgatgg gcaaaagttg catggcacac 27480 cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc acactcacca tgcatgctgc 27540 cacgtcagct ttcacactat gacaccactg gccatacacg ttgccacctc agctttctcc 27600 tcttctcact atgacacgac tggccatgca tgcatgcatg ctgccacctc agctcccgcc 27660 tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg ccaacctctt ctctctataa 27720 atagagagaa gagaatgatg gttaaactca aaccaaaaca agaaaacata cacaaatagc 27780 aaaacggatc cacaaccaaa aatgcttcag cacacttggc ttccgaagcc tcccaatctg 27840 accctcttgt cagatgaggt tcatctctgg aggattcctc ttgaccagcc tgagtcacaa 27900 cttcaagacc ttgctgccac cctgagcagt gatgaattgg cgagggcaaa cagattctac 27960 tttccagaac acagaaggcg tttcactgct gggagaggca tcctcagatc catcttgggt 28020 ggatacttgg gagtggaacc gggtcaagtc aagtttgatt atgagtcccg tgggaaaccg 28080 atccttggtg acagatttgc tgagagtgga ctcctgttca acttgtctca cagccagaac 28140 cttgccttgt gtgctgtcaa ctacacgcgt caaataggca ttgatcttga atatctgcgt 28200 ccaacatctg acttggagtc tcttgcaaag aggttctttc tcccaagaga atatgaactc 28260 ttgaggtcac tccctgatga gcagaaacag aagattttct ttcgttactg gacttgcaaa 28320 gaggcttatc tcaaagcaac gggagatgga atagccaaac ttgaagagat cgagatagca 28380 ctcaccccaa cagaacctgc caagctccaa acagctcctg cgtggtctct gttggagctt 28440 gtgccagatg acaattgtgt tgcagctgtg gctgttgcgg gttttggttg gcagcccaag 28500 ttctggcatt actgagtagt tagcttaatc acttaggtca cccagtccaa gcccggccta 28560 gcacgatgat catgagtgtt gctattgatg tatgttaata ctatacacag tcatcgtgtg 28620 tgttttataa aactgtaagc ttaggatgtt tgaggctaat gtaattagca ctacaccgta 28680 ataaatgaga ggtttacttt atgttttaat aatgttctta cccttgctgc tctctaaact 28740 gtaatggcgt acggttagct aaactaaagt aaaaatcgat tcgcttgagt aaaattagtg 28800 accgggaaag atcatcgact actactgctc agagatgtaa tcggtaatta agacttcaag 28860 ctcaataatt aattaggagt aaatctggct tcgaattttt aaattgtcaa cttccaaaga 28920 aggattggca agactcaatc tcaattgccc aacaaacata tggctcttta caaatttgga 28980 atctcttttt gatggcaaga acgtcgtaat tcctctgtcg atctcatgta attaccaaac 29040 tcatctactt attactttct ttatatgcac ttactgatgc ggccgcttaa ttaactactg 29100 tcactgaggc cgtagacgag tacggactga tctaactagt gctagcctcg aggtcgacac 29160 ccagctttct tgtacaaagt ggttgcggcc gcttaattaa atttaaattc aattaatgca 29220 atcttgattt tcaacaacga aggtaatggc gtaaaagaaa aaatgtatgt tattgtattg 29280 atctttcatg atgttgaagc gtgccataat atgatgatgt ataattaaaa tattaactgt 29340 cgcattttat tgaaatggca ctgttatttc aaccatatct ttgattctgt tacatgacac 29400 gactgcaaga agtaaataat agacgccgtt gttaaagaat tgctatcata tgtgcctaac 29460 tagagggaat ttgagcgtca gacctaatca aatattacaa aatatctcac tctgtcgcca 29520 gcaatggtgt aatcagcgca gacaaatggc gtaaagatcg cggaaaaacc tccccgagtg 29580 gcatgatagc tgcctctgta ttgctgattt agtcagcctt atttgactta agggtgccct 29640 cgttagtgac aaattgcttt caaggagaca gccatgcccc acactttgtt gaaaaacaaa 29700 ttgcctttgg ggagacggta aagccagttg ctcttcaata aggaatgtcg aggaggcaat 29760 gtaaccgcct ctggtagtac acttctctaa tccaaaaatc aatttgtatt caagataccg 29820 caaaaaactt atggtttaaa ccctgcagga ctagtccaga aggtaattat ccaagatgta 29880 gcatcaagaa tccaatgttt acgggaaaaa ctatggaagt attatgtaag ctcagcaaga 29940 agcagatcaa tatgcggcac atatgcaacc tatgttcaaa aatgaagaat gtacagatac 30000 aagatcctat actgccagaa tacgaagaag aatacgtaga aattgaaaaa gaagaaccag 30060 gcgaagaaaa gaatcttgaa gacgtaagca ctgacgacaa caatgaaaag aagaagataa 30120 ggtcggtgat tgtgaaagag acatagagga cacatgtaag gtggaaaatg taagggcgga 30180 aagtaacctt atcacaaagg aatcttatcc cccactactt atccttttat atttttccgt 30240 gtcatttttg cccttgagtt ttcctatata aggaaccaag ttcggcattt gtgaaaacaa 30300 gaaaaaattt ggtgtaagct attttctttg aagtactgag gatacaactt cagagaaatt 30360 tgtaagtttg taggtaccag atctggatcc caaaccatgt ctccggagag gagaccagtt 30420 gagattaggc cagctacagc agctgatatg gccgcggttt gtgatatcgt taaccattac 30480 attgagacgt ctacagtgaa ctttaggaca gagccacaaa caccacaaga gtggattgat 30540 gatctagaga ggttgcaaga tagataccct tggttggttg ctgaggttga gggtgttgtg 30600 gctggtattg cttacgctgg gccctggaag gctaggaacg cttacgattg gacagttgag 30660 agtactgttt acgtgtcaca taggcatcaa aggttgggcc taggatctac attgtacaca 30720 catttgctta agtctatgga ggcgcaaggt tttaagtctg tggttgctgt tataggcctt 30780 ccaaacgatc catctgttag gttgcatgag gctttgggat acacagcccg gggtacattg 30840 cgcgcagctg gatacaagca tggtggatgg catgatgttg gtttttggca aagggatttt 30900 gagttgccag ctcctccaag gccagttagg ccagttaccc aaatctgagt agttagctta 30960 atcacctaga gctcgatcgg cggcaatagc ttcttagcgc catcccgggt tgatcctatc 31020 tgtgttgaaa tagttgcggt gggcaaggct ctctttcaga aagacaggcg gccaaaggaa 31080 cccaaggtga ggtgggctat ggctctcagt tccttgtgga agcgcttggt ctaaggtgca 31140 gaggtgttag cgggatgaag caaaagtgtc cgattgtaac aagatatgtt gatcctacgt 31200 aaggatatta aagtatgtat tcatcactaa tataatcagt gtattccaat atgtactacg 31260 atttccaatg tctttattgt cgccgtatgt aatcggcgtc acaaaataat ccccggtgac 31320 tttcttttaa tccaggatga aataatatgt tattataatt tttgcgattt ggtccgttat 31380 aggaattgaa gtgtgcttga ggtcggtcgc caccactccc atttcataat tttacatgta 31440 tttgaaaaat aaaaatttat ggtattcaat ttaaacacgt atacttgtaa agaatgatat 31500 cttgaaagaa atatagttta aatatttatt gataaaataa caagtcaggt attatagtcc 31560 aagcaaaaac ataaatttat tgatgcaagt ttaaattcag aaatatttca ataactgatt 31620 atatcagctg gtacattgcc gtagatgaaa gactgagtgc gatattatgg tgtaatacat 31680 aggaattcgt ttaaacgatc tgcgtctaat tttcggtcca acttgcacag gaaagacgtc 31740 gaccgcggta gctcttgccc agcagactgg gcttccagtc ctttcgctcg atcgggtcca 31800 atgttgtcct cagctgtgaa ccggaagcgg acgaccaaca gtggaagaac tgaaaggaac 31860 gagccgtcta taccttgatg atcggcctct ggtgaagggt atcatcgcag ccaagcaagc 31920 tcatgaaagg ctgatggggg aggtgtataa ttatgaggcc cacggcgggc ttattcttta 31980 gggaggatct atctcgttgc tcaagtgcat ggcgcaaagc agttattgga gtgcggattt 32040 tcgttggcat attattcgcc acgagttagc agacgaagag accttcatga acgtggccaa 32100 ggccagagtt aagcagatgt tacgccctgc tgcaggcctt tctattatcc aatagttggt 32160 tgatctttgg aaagagcctc ggctgaggcc catactgaaa gagatcgatg gatatcgata 32220 tgccatgttg tttgctagcc agaaccagat cacatccgat atgctattgc agcttgacgc 32280 agatatggag gataagttga ttcatgggat cgctcaggag tagctcatcc atgcacgccg 32340 acaagaacag aaattccgtc gagttaacgc agccgcttac gacggattcg aaggtcatcc 32400 attcggaatg tattagtttg caccagctcc gcgtcacacc tgtcttcatt tgaataagat 32460 gttagcaatt gtttttagct ttgtcttgtt gtggcagggc ggcaagtgct tcagacatca 32520 ttctgttttc aaattttatg ctggagaaca gcttcttaat tcctttggaa ataatagact 32580 gcgtcttaaa attcagatgt ctggatatag atatgattgt aaaataacct atttaagtgt 32640 catttagaac ataagtttta tgaatgttct tccattttcg tcatcgaacg aataagagta 32700 aatacacctt ttttaacatt acaaataagt tcttatacgt tgtttataca ccgggaatca 32760 tttccattat tttcgcgcaa aagtcacgga tattcgtgaa agcgacataa actgcgaaat 32820 ttgcggggag tgtcttgagt ttgcctcgag gctagcgcat gcacatagac acacacatca 32880 tctcattgat gcttggtaat aattgtcatt agattgtttt tatgcataga tgcactcgaa 32940 atcagccaat tttagacaag tatcaaacgg atgtgacttc agtacattaa aaacgtccgc 33000 aatgtgttat taagttgtct aagcgtcaat ttga 33034 <210> 36 <211> 33003 <212> DNA <213> Artificial sequence <220> <223> Plasmid pDAB7362 T-strand <400> 36 cagtcagcat catcacacca aaagttaggc ccgaatagtt tgaaattaga aagctcgcaa 60 ttgaggtcta caggccaaat tcgctcttag ccgtacaata ttactcaccg gatcctaacc 120 ggtttaatta aggcgcgcca tgcccgggca agcggccgca caagtttgta caaaaaagca 180 ggcttctaga cctaggtgga gtcatcacgc agactatctc agcatgtgcg tagcacgcgg 240 ccgcagctta tagtaaagat ttttttgtta ataaaatgaa agttaatatt tatggattta 300 tttgaacata tttacaagtt tttgttaata aatttattta cagaagtgtt aaatttgaaa 360 atgtgttaaa tgagagttaa tatttatgga tataatatta ctttatttaa aaataaaatg 420 aatttatatt aaaatatgat ttggtaaaag atattataaa gaggaacttg gatgagaaag 480 acgacgatgg gcaaaagttg catggcacac cgcttttgtt cagtaacatc tgcactgtgg 540 cgcctttttc acactcacca tgcatgctgc cacgtcagct ttcacactat gacaccactg 600 gccatacacg ttgccacctc agctttctcc tcttctcact atgacacgac tggccatgca 660 tgcatgcatg ctgccacctc agctcccgcc tcttcacccg tgtctttctc tcatgtcgca 720 gtgctgcgtg ccaacctctt ctctctataa atagagagaa gagaatgatg gttaaactca 780 aaccaaaaca agaaaacata cacaaatagc aaaacggatc cacaaccaaa aatggcagcg 840 aggctgcaag agcagaaagg aggtgagatg gacacgagga ttgcgatcat aggcatgagc 900 gccatacttc cttgtggaac cacggtgaga gagtcatggg agaccataag ggctggcatt 960 gattgcctgt ctgatcttcc tgaggatcgt gttgatgtga ctgcgtactt tgacccagtc 1020 aagaccacaa aggacaaaat ctactgcaag agaggaggtt tcattcctga gtatgacttt 1080 gatgcgcgtg agtttggtct caacatgttc cagatggaag attctgatgc gaatcagacc 1140 atctcacttc tcaaggtcaa agaagcactt caagatgctg ggattgatgc acttggaaag 1200 gagaagaaaa acataggatg cgtgcttggc attggaggtg gacagaagag ttcacatgag 1260 ttctacagca gactcaacta tgtggttgtg gagaaagtct tgagaaagat ggggatgcca 1320 gaggaagatg tcaaagtggc tgtggagaag tacaaagcga acttcccaga atggagactt 1380 gactctttcc ctggattcct tgggaatgtc actgctggca gatgcaccaa cacattcaac 1440 ttggatggca tgaactgcgt tgtggatgct gcatgtgcaa gctccttgat agcagtcaag 1500 gttgcgattg atgaactgct ctatggtgac tgcgacatga tggtcactgg tgcaacctgc 1560 acagacaact ccattgggat gtacatggct ttctcaaaga cacctgtgtt cagcacggac 1620 ccaagtgtga gagcgtatga tgagaaaacg aaagggatgc tcattggtga gggatcagcc 1680 atgttggtgc tcaaacgtta tgcagatgca gtgagggatg gtgatgagat tcatgctgtg 1740 attcgtggtt gtgcaagctc aagtgatggc aaagcagctg gcatctacac tcccacaatc 1800 agtggtcaag aggaagcctt gagacgtgca tacaatcgtg cctgcgttga ccctgccact 1860 gtcactttgg ttgagggtca tgggactggc actccagttg gagataggat tgagttgact 1920 gcgttgagga acttgtttga caaagcgtat ggtgaaggga acacagagaa agttgcagtt 1980 ggaagtatca agtccagtat tggtcatttg aaggctgttg ctggtcttgc tggaatgatc 2040 aaggtcatca tggctctcaa acacaagact ttgcctggaa cgatcaatgt ggacaatcca 2100 cccaatctct atgacaacac tcccatcaat gagtcatccc tctacatcaa cacgatgaac 2160 agaccctggt tccctccacc tggtgttccg agacgtgctg gaatatccag ctttggcttt 2220 ggaggtgcca actaccatgc agtgttggaa gaggctgaac cagagcacac gacagcgtac 2280 agactcaaca aacgtccaca accggtgttg atgatggctg caactcctgc tgcgttgcag 2340 agcctttgtg aagctcagct gaaagagttt gaagctgcga tcaaggagaa tgagacagtc 2400 aagaatactg cctacatcaa gtgtgtcaag tttggagaac agttcaagtt ccctgggagc 2460 atacctgcga ccaatgcgag actgggattc ttggtcaaag atgcggagga tgcgtgctcc 2520 acgcttcgtg ctatatgtgc tcagtttgcc aaagatgtga ccaaagaagc gtggaggctt 2580 ccgagagaag gagtttcttt ccgtgccaaa ggcattgcca ccaatggtgc tgttgctgca 2640 ctcttctctg gacaaggtgc tcagtacaca cacatgttca gtgaagttgc gatgaactgg 2700 cctcagttca gacagtccat tgctgcaatg gacgctgcac agtccaaggt tgctggaagt 2760 gacaaagact ttgaaagagt ctctcaagtc ctgtacccaa ggaaaccgta cgaacgtgag 2820 ccagaacaag atcacaagaa aatctcactc acagcataca gccagccgtc aaccctggca 2880 tgtgctcttg gtgcctttga gattttcaag gaagctggat tcactccaga ctttgcagct 2940 ggacacagct tgggtgagtt tgctgcgctc tatgcagctg gttgtgttga cagagatgag 3000 ttgtttgaac ttgtttgcag aagggcaagg atcatgggag gcaaagatgc acctgccaca 3060 cccaaaggtt gcatggcagc tgtcatagga cccaacgcag agaacatcaa ggttcaagct 3120 gccaacgtct ggcttggaaa ctcaaactct ccgtctcaga ctgtcattac tgggtctgtt 3180 gaagggattc aagctgaaag tgccagattg cagaaggaag ggttcagagt tgtgcctctt 3240 gcttgtgagt ctgccttcca ttctccacag atggaaaatg ccagctcagc tttcaaagat 3300 gtcatatcca aggtttcctt ccgtacaccc aaagcagaaa caaagctctt cagcaatgtt 3360 tctggagaga cctatccgac ggatgccaga gagatgctca ctcaacacat gacctcatct 3420 gtgaagttcc tgacccaagt gaggaacatg caccaagctg gtgcgagaat ctttgttgag 3480 tttggaccga aacaagttct cagcaaactt gtttctgaaa ccctcaaaga tgacccgtct 3540 gttgtcacag tgtctgtgaa cccagcttct ggcacggact ctgacataca gttgagggat 3600 gctgcagttc agctggttgt ggctggtgtc aatcttcaag ggtttgacaa gtgggatgct 3660 cctgatgcca cgaggatgca agccatcaag aaaaagagaa ccacactcag actttcagct 3720 gccacctatg tcagtgacaa gaccaagaaa gtgagggatg ctgccatgaa cgacggaaga 3780 tgtgtcacat acctcaaggg tgctgcacca ctcatcaagg ctcctgaacc tgtggttgac 3840 gaagctgcca agagggaggc tgaaaggctt cagaaggagc ttcaagacgc tcagaggcag 3900 cttgacgacg ccaaacgtgc agctgcagaa gccaactcca aacttgctgc tgccaaggag 3960 gaagccaaga ctgctgcagc ttcagccaag cctgcagttg acactgctgt tgtggagaaa 4020 catcgtgcca tcctcaagtc catgctggct gagctggatg gttatggaag tgttgatgct 4080 tcttcccttc agcaacagca acagcaacag actgctccgg cgcctgttaa agccgctgcc 4140 cctgccgctc ccgttgcaag tgctcctgct cccgccgtgt ccaacgagtt gcttgagaaa 4200 gctgagacag ttgtgatgga ggttctcgct gccaagactg gatacgaaac agacatgatt 4260 gaggcggaca tggagcttga gactgagttg ggaattgaca gcatcaagag ggttgaaatt 4320 ctttctgaag tccaagctat gcttaacgtt gaggcgaaag atgttgatgc tctctctagg 4380 acgagaacag tgggtgaagt tgtcaacgct atgaaggcag aaatcgctgg ttcttccgct 4440 ccggcgccgg cagctgccgc acctgcaccg gctaaagcgg ctccagctgc agctgcacca 4500 gctgtttcta atgagctgct cgaaaaggca gaaactgttg tgatggaagt ccttgctgcc 4560 aaaactggct acgaaactga catgatagaa tcagacatgg agcttgagac ggagcttgga 4620 attgattcca tcaagcgtgt ggaaatcctc agtgaagtcc aagccatgct caatgtggaa 4680 gctaaggatg tggatgctct ctcaaggacc agaactgtcg gtgaggttgt caatgccatg 4740 aaggctgaga tagctggagg ctccgctcct gcgccggccg ctgccgctcc tggtcccgct 4800 gccgcggctc cagctccagc tgcagccgct ccggccgtct caaacgaact ccttgaaaaa 4860 gctgagaccg tcgtgatgga ggtcttggca gctaaaaccg gctatgagac tgacatgatt 4920 gagtctgaca tggaattgga gacagaactt gggatagaca gcatcaaacg tgttgaaatc 4980 ctcagtgagg tccaagctat gctgaacgtg gaagcgaagg atgttgatgc tctgtccaga 5040 acgaggaccg ttggtgaggt tgtggatgcc atgaaggctg aaattgctgg agggtctgcg 5100 cccgcgccag ctgcagccgc tcctgcaccc gcagccgcgg cacctgctcc agctgcacct 5160 gcacctgccg tttcttcaga actcttggag aaggctgaga cggttgtgat ggaggttttg 5220 gctgcaaaga ctggctatga gaccgacatg attgaatctg atatggaatt ggagacagag 5280 cttgggatcg attccatcaa aagagtggag atcctgagtg aagtccaagc catgctcaat 5340 gttgaagcta aggatgttga tgcactttca agaacaagga cagtgggtga ggttgtggat 5400 gccatgaagg cggagattgc tggagggagc gcgcctgcgc cagctgccgc ggcacctgca 5460 ccggcagccg cagctccagc tccagctgca ccagcaccgg ccgcgcccgc gccagctgtt 5520 tcatccgaac tcctggaaaa ggccgagacg gttgtgatgg aggtgctcgc agctaagact 5580 ggttacgaga ccgacatgat tgaaagtgac atggagcttg aaaccgagtt gggaatagat 5640 agcatcaaac gtgttgaaat cttgtctgag gtccaagcta tgttgaacgt ggaggcaaaa 5700 gatgtcgatg cgctttcaag aaccagaaca gtcggtgagg tcgtggacgc catgaaggct 5760 gaaattgccg gaagctctgc tagtgcgcca gctgcagccg cacctgcacc tgctgccgcg 5820 gcacctgctc cagctgcagc cgctcctgcc gtcagcaatg agttgctgga gaaagccgaa 5880 acagttgtga tggaagtcct tgctgcaaaa actggttacg aaacggacat gattgagtca 5940 gacatggaat tggagaccga acttggaata gattccatca agagagtgga gatcctctct 6000 gaggtgcaag ccatgctcaa cgttgaggct aaagatgttg atgcactcag taggacaaga 6060 actgtggggg aagttgtcga tgcgatgaag gctgagattg ctggaggctc tgctcctgcg 6120 ccggcagctg ccgcacctgc acccgcagcc gcggcaccag ctgtcagcaa cgaattgctt 6180 gagaaggctg aaacagttgt catggaagtg ctggctgcaa aaaccggcta tgagactgac 6240 atgattgagt ctgacatgga acttgagacg gaattgggca ttgacagcat caagcgtgtt 6300 gagatcctca gtgaagtcca agcaatgctc aatgtggagg ctaaggatgt ggatgccctc 6360 tcaaggacca gaacagttgg tgaggtcgtt gatgcgatga aggcggagat cgctggaagt 6420 tccgcgcctg cgccagctgc agccgctcct gcacccgcag ccgcggcacc ggctccagct 6480 gcagccgcac cggccgtctc ctcagaactc ctggaaaagg ctgagacagt tgtgatggaa 6540 gtccttgctg cgaaaactgg ctatgagacc gacatgattg aatctgacat ggaactggag 6600 accgagcttg gaattgactc catcaaacgt gttgaaatcc tctctgaggt tcaagcgatg 6660 ttgaatgtgg aggccaaaga tgttgatgct ctttccagaa caaggacggt gggagaggtg 6720 gttgatgcca tgaaggctga gatagctggg ggttctgctc ctgcgccagc tgcagccgct 6780 cctgctcccg ctgccgcggc accagctgtc agcaacgaac tccttgaaaa agctgaaact 6840 gttgtgatgg aggttttggc tgcaaagact ggatacgaga ctgacatgat agagtcagac 6900 atggagttgg aaacagagct tgggattgat agcatcaagc gtgtggaaat cctttctgag 6960 gttcaagcca tgctgaacgt tgaggccaaa gatgtcgatg ctttgtcaag gaccagaacg 7020 gttggagaag tggtcgatgc catgaaggct gagatagctg gtggatcagc ccctgctccg 7080 gctgcagccg ctcctgcaag tgccggtgct gcaccagctg tgaagattga ttcagttcat 7140 ggtgctgact gtgacgattt gtcactgatg cacgcaaagg tggttgacat cagaaggcca 7200 gatgaactga ttctggaaag accggagaac cgtccagttc ttgtcgttga cgatggctca 7260 gagttgacac ttgctcttgt gagggtgttg ggagcctgtg cagttgtctt gacctttgag 7320 ggattgcagt tggctcagag agctggtgca gctgccattc gtcatgttct ggctaaggac 7380 ctttcagcag aatcagctga gaaggccatc aaagaggctg agcaaagatt tggagcgttg 7440 ggaggcttca taagccagca agcagaaagg tttgaaccag ctgagatact gggtttcaca 7500 ctcatgtgtg cgaagtttgc caaagcctct ctctgcactg ctgtggctgg aggtcgtcct 7560 gccttcatcg gtgtggctag gcttgacggc agacttggat tcacctctca aggcacttct 7620 gacgcactca agagagcaca gcgtggagcg atctttggac tctgcaagac aatagggttg 7680 gagtggtcag aatctgatgt gttcagccgt ggtgtggaca ttgctcaagg gatgcaccca 7740 gaagatgctg cagttgccat agttagggag atggcgtgtg ctgacatcag aatcagagag 7800 gttgggattg gagcaaacca gcaaagatgc acaatcagag ctgccaagtt ggagactggg 7860 aaccctcaga gacagatagc gaaagacgat gtgcttctgg tttctggagg tgctcgtggg 7920 atcacacctc tttgcatccg tgagatcacg cgtcagattg ctggaggcaa gtacattctg 7980 ttgggtcgta gcaaagtttc tgcgtctgaa ccagcatggt gtgctggcat cactgatgag 8040 aaggctgtgc agaaagctgc cacccaagag ttgaagaggg ccttcagtgc tggtgaagga 8100 cccaaaccca ctccaagggc tgtcacgaaa cttgtgggat ctgttcttgg tgctcgtgaa 8160 gtgagaagct ccattgctgc cattgaagct ctgggtggca aagcgatcta cagctcttgt 8220 gatgtgaact ctgctgcaga tgttgccaaa gcagttaggg atgcagagtc ccagcttggt 8280 gccagagtta gtggaatcgt tcacgcttct ggtgtgctcc gtgatcgtct catagagaag 8340 aaactccctg acgagtttga tgctgtcttt gggaccaaag tcactgggtt ggagaatctt 8400 ctggctgcag ttgatcgtgc caacctcaaa cacatggttc tcttcagctc ccttgctggt 8460 ttccacggca atgttggtca gtctgactat gcgatggcaa acgaagctct caacaagatg 8520 ggtttggaac ttgcgaagga tgtcagtgtc aagtccatct gctttggtcc ctgggatggt 8580 gggatggtga cacctcaact caagaaacag tttcaagaaa tgggagttca gatcattcct 8640 cgtgaaggag gtgctgatac agttgcgagg attgtgttgg gatcaagtcc tgctgagatc 8700 cttgttggaa actggaggac accatccaag aaagttggaa gtgacacgat caccctgcat 8760 agaaagatca gtgccaagtc caatcccttc ttggaggatc atgtcataca aggcagaagg 8820 gtgttgccaa tgacccttgc tattgggagc cttgctgaga cctgccttgg actcttccct 8880 ggctactctc tctgggccat agatgacgca cagctcttca aaggtgtcac tgttgatgga 8940 gacgtcaact gtgaagtgac cctcacaccg tccactgctc cgagtgggag ggtgaatgtc 9000 caagcgacgc tcaagacgtt ctcaagtgga aaacttgttc cagcctacag agctgtcata 9060 gtgttgtcca accaaggtgc tccacctgcc aatgcaacca tgcagcctcc gagccttgat 9120 gcagatcctg ctttgcaagg gtcagtctat gatggcaaga ctctctttca tggtcctgcg 9180 ttcagaggca ttgatgacgt tctcagctgc accaagagcc agcttgttgc caagtgttct 9240 gctgttcctg gatcagatgc agccagagga gagtttgcaa ctgacactga tgcacatgat 9300 ccctttgtca atgatcttgc ctttcaagca atgttggtct gggtgaggag aacgctggga 9360 caagctgcac tcccgaactc cattcagagg atagtccaac acagaccggt cccacaagac 9420 aaacccttct acatcaccct cagatccaat cagagtggtg gacatagcca gcacaaacat 9480 gcacttcagt tccacaacga acaaggtgat ctcttcattg atgttcaagc ctctgtcatt 9540 gcgacagact cacttgcgtt ctgagtagtt agcttaatca cttaggtcac ccagtccaag 9600 cccggcctag cacgatgatc atgagtgttg ctattgatgt atgttaatac tatacacagt 9660 catcgtgtgt gttttataaa actgtaagct taggatgttt gaggctaatg taattagcac 9720 tacaccgtaa taaatgagag gtttacttta tgttttaata atgttcttac ccttgctgct 9780 ctctaaactg taatggcgta cggttagcta aactaaagta aaaatcgatt cgcttgagta 9840 aaattagtga ccgggaaaga tcatcgacta ctactgctca gagatgtaat cggtaattaa 9900 gacttcaagc tcaataatta attaggagta aatctggctt cgaattttta aattgtcaac 9960 ttccaaagaa ggattggcaa gactcaatct caattgccca acaaacatat ggctctttac 10020 aaatttggaa tctctttttg atggcaagaa cgtcgtaatt cctctgtcga tctcatgtaa 10080 ttaccaaact catctactta ttactttctt tatatgcact tactgatgcg gccgcttaat 10140 taagatgagt gatactcagg actcaggact cactctgctg atcactagtg ctagcctcga 10200 ggtcgacaca actttgtata caaaagttgt ctagacctag gctcagcaac tcatccagtc 10260 aagttcgagt cagtgagttg ggcggccgca gcttatagta aagatttttt tgttaataaa 10320 atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt taataaattt 10380 atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt atggatataa 10440 tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt aaaagatatt 10500 ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg cacaccgctt 10560 ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat gctgccacgt 10620 cagctttcac actatgacac cactggccat acacgttgcc acctcagctt tctcctcttc 10680 tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc ccgcctcttc 10740 acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc tataaataga 10800 gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa atagcaaaac 10860 ggatccacaa ccaaaaatgg ctgcaaggaa cgtgagtgcg gctcatgaga tgcatgacga 10920 gaagaggata gctgttgtgg gcatggctgt tcagtatgct ggatgcaaaa cgaaagacga 10980 gttctgggaa gttctcatga atggaaaagt tgagagcaaa gtgatcagcg acaaacgtct 11040 tggttcaaat taccgtgctg aacactacaa agctgaaagg agcaagtatg ctgacacctt 11100 ctgcaatgag acatatggaa ctcttgatga gaacgaaata gacaatgagc atgaactcct 11160 tttgaacctt gccaaacaag ccttggctga aacatctgtc aaagattcaa caagatgtgg 11220 catagtttct ggatgcctca gcttcccgat ggacaacctc caaggtgaac tcttgaatgt 11280 gtaccagaac catgttgaga agaaacttgg tgccagagtt ttcaaagatg cctctcactg 11340 gtcagaaaga gaacagtcca acaaaccaga ggctggtgac agaagaatct tcatggaccc 11400 tgcttccttt gttgctgagg aactcaatct tggcgctctc cactactctg ttgatgctgc 11460 ctgtgccact gcgctctatg tgctcagact ggcgcaagat cacttggtct ctggtgcagc 11520 tgatgtgatg ctttgtggtg caacatgcct gccggagccg tttttcatac tctctgggtt 11580 ctcaaccttc caagccatgc ctgttggaac gggtcagaat gtgtccatgc cgctgcacaa 11640 agattctcaa ggattgaccc ctggtgaagg aggttccatc atggtcctca aacgtttgga 11700 tgacgcaatc agagatggag atcacatcta tggaacactt ttgggagcca atgtcagcaa 11760 cagtggcact ggtctgccac ttaaacccct tttgccgtca gagaaaaagt gcctcatgga 11820 cacctacaca cgtatcaatg tgcatcccca caagattcag tatgttgaat gccatgcaac 11880 tgggactcca caaggtgatc gtgtggaaat tgatgctgtg aaggcttgct ttgaaggcaa 11940 agtcccacgt tttggaacca caaaagggaa ctttggacac acattggtgg cagctgggtt 12000 tgctggcatg tgcaaagtgc ttctctccat gaagcacggg atcattccac ccactccggg 12060 aatagatgac gaaacgaaga tggaccctct ggttgtctct ggtgaggcca ttccctggcc 12120 agaaacgaat ggagaaccga agcgtgctgg tctctcagcg tttggatttg gtgggaccaa 12180 tgctcatgct gtctttgagg aacatgaccc aagcaatgct gcatgcactg gccatgactc 12240 catttctgct ctctctgcac gttgtggtgg tgagtcaaac atgagaatag caatcactgg 12300 gatggatgcc acttttggtg ctttgaaagg attggatgct tttgaaaggg ccatctacac 12360 tggtgctcat ggtgctatac cactcccaga aaagagatgg agattcctgg ggaaagacaa 12420 ggacttcctt gatctttgtg gagtcaaagc aacaccgcat ggctgttaca ttgaagatgt 12480 tgaagttgat ttccagaggc tgaggactcc aatgactcct gaagatatgc tcttgcctca 12540 acagcttctg gctgtcacca caattgatag ggccatattg gactctggca tgaagaaagg 12600 tggaaatgtg gctgtctttg tggggttggg gaccgatctt gaactctacc gtcaccgtgc 12660 aagggttgct ctcaaggaga gagtgaggcc tgaagccagc aagaaactca atgacatgat 12720 gcagtacatc aatgattgtg ggacaagcac ttcttacacc agctacatag gcaacttggt 12780 tgcaacaagg gtcagttcac aatggggctt cacgggacct tccttcacaa tcacagaagg 12840 caacaattct gtgtacagat gtgctgaact tggcaaatac ctcttggaaa cgggagaggt 12900 tgatggggtt gtggttgcgg gtgttgatct ctgtgggagt gctgagaacc tttacgtcaa 12960 gtcaaggcgt ttcaaggtgt ctacatctga caccccgaga gccagctttg atgcagctgc 13020 tgatggttac tttgttggag agggctgtgg tgcatttgtg ctcaagcgtg agacaagctg 13080 caccaaggat gacagaatct atgcttgcat ggatgccata gttcctggaa atgttccatc 13140 tgcatgtttg agagaggcat tggatcaagc cagagtcaag cctggagaca tcgagatgtt 13200 ggagctttct gctgattcag cgaggcattt gaaggacccg tctgttctcc caaaggaact 13260 cactgctgag gaagagattg gcggacttca gacgattctt cgtgacgatg acaagctccc 13320 aaggaatgtt gcaactggaa gtgtcaaggc cactgttggt gacactggtt atgcttctgg 13380 tgctgccagt ttgattaagg ctgcgctttg catctacaac cgttacctgc ccagcaatgg 13440 agatgactgg gatgaacctg cacctgaagc accctgggac agcacactct ttgcgtgcca 13500 gacctcaaga gcttggttga agaaccctgg tgagagacgt tatgcggctg tttccggagt 13560 cagtgaaacc agaagttgct acagtgtgct gctttctgaa gcagagggac actacgagag 13620 agagaacaga atcagtttgg atgaggaagc acccaagctc attgtgttga gagcagactc 13680 ccatgaagag attcttggaa gattggacaa gataagggaa aggttcttgc agcccacggg 13740 tgctgcaccc agagagtctg aattgaaggc gcaagcccgt aggatctttc ttgaactcct 13800 tggtgagacc cttgcccaag atgcagcttc atctggaagt cagaagcccc ttgctctcag 13860 cttggtctcc actccatcca agctccagag agaggtggaa cttgcggcaa aaggcattcc 13920 gagatgtctc aagatgagac gtgattggag ttcccctgct ggaagcagat atgcgcctga 13980 gcctttggcc agtgacagag ttgccttcat gtatggagaa ggaaggagcc cttactatgg 14040 catcacacaa gacattcata ggatttggcc tgaacttcat gaggtcatca atgagaaaac 14100 aaaccgtctc tgggctgaag gtgacagatg ggtcatgcca agggccagct tcaagagtga 14160 gcttgagtca caacagcaag agtttgacag aaacatgatt gaaatgttcc gtcttggaat 14220 cctcacctca attgccttca caaacctggc gagagatgtt ctcaacatca cgcccaaagc 14280 agcctttgga ctcagtttgg gagagatttc catgatcttt gctttctcca agaaaaacgg 14340 tttgataagc gatcagttga caaaggattt gagagagtca gatgtctgga acaaagctct 14400 ggctgttgag ttcaatgcgc tcagagaagc ctggggcatt ccacagtctg tcccaaaaga 14460 tgagttctgg caaggttaca tagtgagggg aacgaaacaa gacatagaag cagccatagc 14520 accagactca aagtatgtga ggttgaccat catcaatgat gccaacactg ctctcatcag 14580 tggaaagcct gatgcatgca aagctgcgat tgctcgtctt ggtggcaaca tccctgcgct 14640 tccagtcact caaggcatgt gtgggcattg cccagaggtg ggaccttaca ccaaggacat 14700 cgcaaagatc cacgccaacc ttgagttccc tgtggttgat ggtcttgatc tctggactac 14760 cataaaccag aagaggcttg tgccaagagc cacgggtgcc aaagatgaat gggctccttc 14820 atcctttggg gagtatgctg gacagctcta tgagaaacaa gccaacttcc ctcagatagt 14880 tgaaaccatc tacaagcaga actatgatgt gtttgttgaa gttgggccaa acaatcacag 14940 aagcacagca gtgagaacca cgcttggacc acagagaaac caccttgctg gtgccataga 15000 caagcagaac gaggatgctt ggacgacaat agtcaagctc gtggcttcat tgaaggctca 15060 ccttgttcct ggagtgacca tcagtccgct ctaccactcc aagctcgttg cagaggctga 15120 ggcttgttat gctgcgctct gtaaaggtga gaaacccaag aaaaacaagt ttgtgagaaa 15180 gattcagttg aatggcagat tcaacagcaa ggcagacccc atctcctcag cagaccttgc 15240 atccttccca cctgcggacc ctgcgattga agctgccatc tcttcaagga tcatgaagcc 15300 tgtggctccg aaattctatg cgcgtctcaa cattgatgag caagatgaga ccagagatcc 15360 gatcttgaac aaggacaatg cgccttccag ctccagctct tccagcagtt cctcttccag 15420 cagttcatct ccgtctcctg ctccttcagc tccagttcag aagaaagctg ccccagctgc 15480 ggagacaaaa gctgttgcca gtgctgatgc acttcgtagt gccctgcttg atcttgactc 15540 catgcttgcg ctcagctctg caagtgcttc tggcaacctt gttgagactg cgccatctga 15600 tgccagtgtc attgtccctc cgtgcaacat tgctgatctt ggcagcagag ctttcatgaa 15660 aacttatggt gtttctgcgc ctctgtacac cggtgcaatg gccaagggaa ttgcctctgc 15720 ggacttggtg atcgcagctg gaaggcaagg aatccttgcg tcatttggtg ctggaggcct 15780 tccaatgcaa gttgtgcgtg agtccattga aaagattcaa gctgccctgc ccaatggacc 15840 atatgctgtc aacctcatcc attctccttt tgacagcaac cttgagaagg gaaatgttga 15900 tctcttcttg gaaaagggtg tcacttttgt tgaggcttca gccttcatga cgttgacacc 15960 gcaagttgtc agatacagag cggctgggtt gacgaggaat gcagatgggt cagtcaacat 16020 aaggaacaga atcattggga aagtctccag aactgagctg gctgaaatgt tcatgcgtcc 16080 tgcacctgag catcttttgc agaagctcat tgcttctggg gagatcaatc aagaacaagc 16140 tgagttggcc agacgtgttc ctgtggctga tgacattgcg gttgaagctg attctggtgg 16200 acacactgac aaccgtccaa tccatgtcat tctgcctctg attatcaacc tgagggatag 16260 gcttcacaga gagtgtggat acccagcaaa cctgagggtt cgtgttggtg ctgggggtgg 16320 cattgggtgt ccccaagctg cgctggcaac tttcaacatg ggtgcctctt tcattgtcac 16380 tggaacagtg aaccaagttg ccaaacaatc tgggacgtgt gacaatgtga ggaaacagct 16440 tgcgaaagca acttactcag atgtttgcat ggcaccggca gctgacatgt ttgaggaagg 16500 tgtgaaactt caagttttga agaaaggaac aatgttccct tcaagagcca acaaactcta 16560 tgagttgttc tgcaagtatg attcttttga gtccatgcct ccagcagagc ttgcgagggt 16620 tgagaaaaga atcttcagtc gtgcattgga agaggtctgg gatgagacca agaacttcta 16680 catcaaccgt ctccacaacc cagagaagat acagagggct gagagggacc ccaaactgaa 16740 gatgtcactg tgtttcagat ggtatctgtc cctggcttca aggtgggcca acactggagc 16800 ttctgacaga gtcatggact accaagtttg gtgtggtcct gccattggtt cattcaatga 16860 tttcatcaaa ggaacttacc ttgatccggc agtggcaaat gagtacccgt gtgttgtgca 16920 gatcaacaaa cagattctta gaggagcgtg cttcttgagg cgtcttgaaa tactgagaaa 16980 tgcaaggctt tcagatggtg cagctgcact tgtggcgagc attgatgaca catatgtccc 17040 tgctgagaag ctgtgagtag ttagcttaat cacttaggtc acccagtcca agcccggcct 17100 agcacgatga tcatgagtgt tgctattgat gtatgttaat actatacaca gtcatcgtgt 17160 gtgttttata aaactgtaag cttaggatgt ttgaggctaa tgtaattagc actacaccgt 17220 aataaatgag aggtttactt tatgttttaa taatgttctt acccttgctg ctctctaaac 17280 tgtaatggcg tacggttagc taaactaaag taaaaatcga ttcgcttgag taaaattagt 17340 gaccgggaaa gatcatcgac tactactgct cagagatgta atcggtaatt aagacttcaa 17400 gctcaataat taattaggag taaatctggc ttcgaatttt taaattgtca acttccaaag 17460 aaggattggc aagactcaat ctcaattgcc caacaaacat atggctcttt acaaatttgg 17520 aatctctttt tgatggcaag aacgtcgtaa ttcctctgtc gatctcatgt aattaccaaa 17580 ctcatctact tattactttc tttatatgca cttactgatg cggccgctta attaatctga 17640 cctgaccact agacctgagt gagtacagat gtgctactag tgctagcctc gaggtcgacc 17700 aacttttcta tacaaagttg tctagaccta ggactaggca ctgagtgact cactgacctg 17760 actgagtgac tggcggccgc agcttatagt aaagattttt ttgttaataa aatgaaagtt 17820 aatatttatg gatttatttg aacatattta caagtttttg ttaataaatt tatttacaga 17880 agtgttaaat ttgaaaatgt gttaaatgag agttaatatt tatggatata atattacttt 17940 atttaaaaat aaaatgaatt tatattaaaa tatgatttgg taaaagatat tataaagagg 18000 aacttggatg agaaagacga cgatgggcaa aagttgcatg gcacaccgct tttgttcagt 18060 aacatctgca ctgtggcgcc tttttcacac tcaccatgca tgctgccacg tcagctttca 18120 cactatgaca ccactggcca tacacgttgc cacctcagct ttctcctctt ctcactatga 18180 cacgactggc catgcatgca tgcatgctgc cacctcagct cccgcctctt cacccgtgtc 18240 tttctctcat gtcgcagtgc tgcgtgccaa cctcttctct ctataaatag agagaagaga 18300 atgatggtta aactcaaacc aaaacaagaa aacatacaca aatagcaaaa cggatccaca 18360 accaaaaatg gcgcttcgtg tcaagaccaa caagaagcca tgttgggaga tgaccaagga 18420 ggaactgacc tctggcaaaa ctgaggtgtt caactatgag gaactcctgg agtttgcaga 18480 gggtgacata gctaaggttt ttggtccgga gtttgctgtc atagacaagt accccagaag 18540 ggtgaggctg cctgctcgtg aatacctgct tgtgacaagg gtcacactca tggatgccga 18600 ggtcaacaac tacagagttg gtgccagaat ggtcactgag tatgatcttc cagtgaatgg 18660 cgaattgtct gagggaggtg attgtccctg ggcagttctg gttgagagtg gacagtgcga 18720 tttgatgctg atatcataca tgggaattga tttccagaac caaggtgaca gagtttaccg 18780 tctgctcaac accacgttga cattctatgg ggttgcccat gagggagaga cccttgagta 18840 tgacatcaga gtgaccggtt ttgccaaacg tttggatggt ggcatctcca tgttcttctt 18900 tgagtatgac tgctatgtca atgggaggct tctcattgag atgagagatg gctgtgctgg 18960 cttcttcaca aatgaggagc ttgatgcggg aaagggtgtg gtgttcacaa gaggggattt 19020 ggctgccaga gctaagattc caaagcaaga tgtttctccc tatgctgtcg ctccctgcct 19080 tcacaaaaca aaactcaacg aaaaggagat gcagaccttg gttgacaaag attgggcttc 19140 cgtttttggc tcaaagaatg gcatgccgga gatcaactac aaactctgcg cacgtaagat 19200 gctcatgatt gatagagtca ccagcattga ccataaggga ggtgtgtacg gtttgggtca 19260 gttggttggt gagaaaatct tggagaggga ccattggtac ttcccatgcc attttgttaa 19320 ggatcaagtc atggctggat ctcttgtcag cgacggttgt tcacaaatgc tcaagatgta 19380 catgatctgg ctcggtttgc accttaccac cggaccattt gacttcagac ccgtcaacgg 19440 acacccgaac aaagttaggt gtaggggtca gatatctcct cataagggaa agttggtcta 19500 cgtcatggag atcaaagaga tgggattcga cgaggataac gatccgtacg ccattgctga 19560 cgtcaacatc attgacgttg atttcgaaaa gggtcaagat ttcagccttg atagaatcag 19620 cgactacgga aagggggatc ttaacaaaaa gatcgtcgtg gacttcaagg gcattgctct 19680 caagatgcag aagagatcca cgaacaagaa cccctccaaa gttcagcccg tttttgctaa 19740 cggtgcagcc actgtgggac cggaagcgtc caaggccagc tctggtgcca gtgctagtgc 19800 ttctgctgcc cctgcgaagc ctgctttctc agcagatgtt cttgcgccaa agccagttgc 19860 acttcctgaa catatcctca agggtgacgc tctcgctccg aaagaaatgt cctggcatcc 19920 catgtcaaaa cttcctggca acccgacgcc gttcttctcc cctagttctt accctccgag 19980 ggcaatttgc ttcatccctt tccctggcaa cccacttgac aacaattgca aagctggaga 20040 aatgcctctg aactggtaca acatgtcaga gttcatgtgt ggaaaagtta gcaactgctt 20100 gggaccagag ttcgcaagat ttgataagtc aaatacgagc agaagccctg cttttgattt 20160 ggctcttgtg actagagttg ttgaagtcac aaacatggaa catggcaagt tcctgaatgt 20220 tgattgcaat cccagcaaag gcacaatggt gggggagttt gactgtcctc aagatgcgtg 20280 gttctttgat ggttcttgca atgatgggca catgccttat tccatcatca tggaaatcgg 20340 acttcagaca tccggtgttc tcaccagtgt gttgaaagca ccactgacga tggacaaaga 20400 tgacattctc tttaggaacc ttgatgcaag tgctgaaatg gtgaggccag atgtggatgt 20460 gagggggaaa accataagga atgtgaccaa gtgcactgga tatgcaatgt tgggaaagat 20520 ggggattcac agattcacgt ttgagttgtc tgttgatggc gtggttttct acaaaggaag 20580 cacttccttt ggatggttca ctccagaggt gtttgctcag caagctggac ttgacaatgg 20640 gaaaaagacg gagccttggt gcaaaactaa caacacgagt gtgagaagag ttgaaatcgc 20700 atcagccaaa ggcaaagaac agctgactga gaaacttcca gatgcaacta atgctcaagt 20760 tctcagaagg tcagaacagt gtgagtattt ggattacctc aacattgctc ctgattctgg 20820 gctgcatggg aaaggatatg cgcatggaca caaagatgtt aacccacaag actggttctt 20880 ctcttgtcac ttttggtttg atcctgtgat gcctggatct ttgggaattg agtcaatgtt 20940 ccagctcatt gaggcatttg cggtggacca gaacataccg ggagaataca atgtgagcaa 21000 tccgaccttt gcccatgccc ctggaaaaac ggcgtggaag taccgtggtc aattgacacc 21060 aaagaaccgt gcgatggact gtgaggtgca catagtttca atcacagcct ctcctgaaaa 21120 tggaggctac gttgacatag tggcagatgg agcactttgg gttgatggtc tccgtgtgta 21180 tgaagccaaa gaactcagag ttcgtgttgt cagtgcaaaa cctcaagcca ttccggatgt 21240 tcaacaacag ccacccagcg caaaagcgga ccctgggaaa actggagttg cactcagtcc 21300 gactcagttg agagatgtcc tgcttgaagt ggacaatcca ttgtatcttg gtgttgaaaa 21360 ctccaatttg gtgcagtttg aatccaaacc tgcaacttct tcaaggattg tcagtatcaa 21420 accatgctcc ataagtgatc ttggtgacaa gtccttcatg gaaacgtaca atgtgtcagc 21480 acctctgtac actggagcaa tggccaaggg aattgcgtct gcggaccttg tcattgctgc 21540 tgggaagagg aagatattgg gttcttttgg agcgggtggg cttccaatgc accatgtgag 21600 ggcagctttg gagaagatac aagcggctct gcctcaagga ccatatgctg tgaacctcat 21660 tcattcacct tttgacagca acttggaaaa ggggaatgtt gatctcttcc tcgaaaaggg 21720 cgttactgtg gtggaggctt ctgcattcat gaccctcact ccccaagttg ttcgttaccg 21780 tgcagccgga ctctcaagga acgctgatgg gagtgtcaac atcagaaacc gtatcattgg 21840 aaaagtgtcc agaacagaac tcgccgaaat gttcatcaga ccggctccgg aacacctcct 21900 tgagaaactc atagcttctg gtgagataac acaagaacaa gccgaacttg cccgtcgtgt 21960 tcctgtcgca gacgacatcg ctgttgaagc tgattctggt ggtcacactg acaaccgtcc 22020 catccacgtc atcttgcctt tgatcatcaa ccttaggaac agactgcaca gagagtgtgg 22080 ttatcctgcg caccttagag tcagagttgg agctggaggc ggtgttggct gcccacaagc 22140 tgctgcggct gccctcacaa tgggagctgc cttcatagtg actgggactg tcaatcaagt 22200 tgctaaacag tccgggacat gtgacaacgt gagaaaacaa ctcagtcaag ccacctacag 22260 tgacatttgc atggctccag cagctgatat gtttgaggaa ggtgtgaaac tccaagttct 22320 caagaaggga acaatgttcc catcaagagc taacaaattg tacgaactct tttgcaagta 22380 cgactctttc gattcaatgc ctcctgctga acttgaaagg atcgagaaga ggatcttcaa 22440 gagggcactt caagaggtct gggaagagac caaggacttt tacatcaatg gtttgaagaa 22500 tccagagaag atacagagag cggaacatga ccccaaactc aagatgtctt tgtgtttcag 22560 atggtatctt ggtcttgcat cacgttgggc caacatgggt gctcctgatc gtgtcatgga 22620 ctatcaagtc tggtgtggtc cagccattgg agccttcaat gacttcatca aaggaacata 22680 tcttgatcct gctgtctcta atgagtaccc atgtgttgtc cagatcaacc ttcagatttt 22740 gcgtggtgca tgttacctga ggcgtcttaa tgctctgaga aatgatccga ggattgatct 22800 tgagactgaa gatgctgcct ttgtctatga gccaacgaat gcgctttgag tagttagctt 22860 aatcacttag gtcacccagt ccaagcccgg cctagcacga tgatcatgag tgttgctatt 22920 gatgtatgtt aatactatac acagtcatcg tgtgtgtttt ataaaactgt aagcttagga 22980 tgtttgaggc taatgtaatt agcactacac cgtaataaat gagaggttta ctttatgttt 23040 taataatgtt cttacccttg ctgctctcta aactgtaatg gcgtacggtt agctaaacta 23100 aagtaaaaat cgattcgctt gagtaaaatt agtgaccggg aaagatcatc gactactact 23160 gctcagagat gtaatcggta attaagactt caagctcaat aattaattag gagtaaatct 23220 ggcttcgaat ttttaaattg tcaacttcca aagaaggatt ggcaagactc aatctcaatt 23280 gcccaacaaa catatggctc tttacaaatt tggaatctct ttttgatggc aagaacgtcg 23340 taattcctct gtcgatctca tgtaattacc aaactcatct acttattact ttctttatat 23400 gcacttactg atgcggccgc ttaattaatc acgagtggat gactgtcacg actgactcat 23460 gactgactac tagtgctagc ctcgaggtcg acacaacttt gtataataaa gttgtctaga 23520 cctaggcgag tcatgagtcg tgacatgacg tcatgctcat cagactgcgg ccgcagctta 23580 tagtaaagat ttttttgtta ataaaatgaa agttaatatt tatggattta tttgaacata 23640 tttacaagtt tttgttaata aatttattta cagaagtgtt aaatttgaaa atgtgttaaa 23700 tgagagttaa tatttatgga tataatatta ctttatttaa aaataaaatg aatttatatt 23760 aaaatatgat ttggtaaaag atattataaa gaggaacttg gatgagaaag acgacgatgg 23820 gcaaaagttg catggcacac cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc 23880 acactcacca tgcatgctgc cacgtcagct ttcacactat gacaccactg gccatacacg 23940 ttgccacctc agctttctcc tcttctcact atgacacgac tggccatgca tgcatgcatg 24000 ctgccacctc agctcccgcc tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg 24060 ccaacctctt ctctctataa atagagagaa gagaatgatg gttaaactca aaccaaaaca 24120 agaaaacata cacaaatagc aaaacggatc cacaaccaaa aatgcccact ccagatgcca 24180 ctgcgcctct caacaagccg tcagactatg ctgtgtacca cgaagaggat ggtcctttct 24240 ggactgctga ttcctctggt gtcagcagag tgaacttctc tgaaactggt gtgggatctg 24300 aaggtgtcat tcctgcgctc acactgattg atgtgtttga gcgtgcggtc aaacgtggtg 24360 gcaataggat tgcattcaga acagagaaca tgccaacctt gaggcgtgga gaagaggctc 24420 ctgatgcttt gccgctcaaa gattggaagt cctggtcttg gaaacagtac aaagctgatg 24480 ttcacagaat tgcgagggct ctgatggatt tgggagttga gcagcacgat gcggtctcca 24540 tctttggctt caactcacca gaatggttcc tcagtgctgt cggtgcagtt catgctggtg 24600 ccaagatcgc tggaatctac ccttcagaca ctcctgctca agtgcagtac aaagccttcc 24660 actctgacac tgctgttgca gttgtggaga acgaacagtg cttcaagaaa ttcgcagagg 24720 tggttgagga tctcccttac ctcaaagcca ttgtttgttg ggattatgaa gccacagaca 24780 tcacaaggga agatggttca gtggttgaag tcctgacctt tgctgagttc ctgaagagag 24840 gtgacactgt ggaagcagct gcacttgatg agagaatcag caagattgag cccaccatgt 24900 gtgctgccct gatctacacc agtggcacaa ctgggagacc caaagctgtc atgatctccc 24960 atgacaacct tgtctttgaa gcatctgctg tggtcccgaa cttgggaggg gcatgcacga 25020 ccactgctga ggaaaggatt ctttcctacc ttcctttgtc tcatgttgct ggaatgatgg 25080 ttgacatcat tgctccaatc atagcgacag cgttccacaa aggaaggatc tgtgtttgtt 25140 ttgccagacc gtatgatttg agaactggaa cccttgggca aagactcaat gcagtggagc 25200 caaccatatt ccttggtgtg ccacgtgtgt gggaaaagat ccaagagaaa ttgatggctg 25260 ttggtgccaa aaccactggt ctcaagaaga aactcagcac ggcagccaag aaacgtgggc 25320 ttgaatttca agaggaacaa cagataggga gaagtggtgc caaccctggc tttggaccgc 25380 ttggaatcta caagaaactt ttgggcctca tcaaaggaaa gttggggctg accaaatgca 25440 agtttgcctt tgctggcgca gctccaatga cccgtgaaac ccttcagtac tttggagcgc 25500 tgaacatcaa catcaatgag gtctatggaa tgtctgaatg cagtggggct gccacctggt 25560 caactgacaa agctcatgag tggggcactg ttggctatga gatgccctcc tgtgaagtca 25620 gagtgttcaa gattgcggag gatggcacca agacagagtg cccaagagct gctgacatca 25680 tgcatgccac agaagaggaa caaggtgaag tttgcttccg tggaaggaac atcatgatgg 25740 gatacttggc caacccgaaa cttggtgatg accatgttgc tgagattgaa gagaagaatg 25800 cagctgccat agatagcgaa gggtggcttc actctggtga caagggagca atttccacaa 25860 ggggaatgct caagataacg ggcagataca aggagcttat cataggtgct ggtggggaga 25920 atgtggctcc tgttcccatt gaagatgcca tcaaggcgag gatgcctttt gtttcaaatg 25980 caatgatggt tggagacaag aggaagttca tggctgtcct tctgactttg aaaacggttg 26040 gagcaactgg agagcttcct ggcacaaaca agctcatggg agcagctgcg gactatggtg 26100 agaccattga ggatgcctgt gacaatgagg cgctcattga agagataacg cagcaactca 26160 aagagatagg tgacgatgga gatgtcacgc caagcaatgc agcccgtata caaaagttca 26220 cgattttgcc actcgacttc agtgtctcca cagatgaact gacagcaacg ttgaaattga 26280 agagaagtgt ggttgcagac aagtatgagg acatcataga ggctttctat gagagcaagt 26340 ctgtctttgt gccttactca acagttggag cttatgcaac gggtggacca gttgatgact 26400 ctgttgtgga tggatcattc aaaggtgact ttagcatgat tggggatgac gatccggatc 26460 ttcagaatgt ggatgtcctt gaagcaattg atgaggacaa ctgagtagtt agcttaatca 26520 cttaggtcac ccagtccaag cccggcctag cacgatgatc atgagtgttg ctattgatgt 26580 atgttaatac tatacacagt catcgtgtgt gttttataaa actgtaagct taggatgttt 26640 gaggctaatg taattagcac tacaccgtaa taaatgagag gtttacttta tgttttaata 26700 atgttcttac ccttgctgct ctctaaactg taatggcgta cggttagcta aactaaagta 26760 aaaatcgatt cgcttgagta aaattagtga ccgggaaaga tcatcgacta ctactgctca 26820 gagatgtaat cggtaattaa gacttcaagc tcaataatta attaggagta aatctggctt 26880 cgaattttta aattgtcaac ttccaaagaa ggattggcaa gactcaatct caattgccca 26940 acaaacatat ggctctttac aaatttggaa tctctttttg atggcaagaa cgtcgtaatt 27000 cctctgtcga tctcatgtaa ttaccaaact catctactta ttactttctt tatatgcact 27060 tactgatgcg gccgcttaat taatctgatc atggatgcta cgtcacggca gtacaggact 27120 atcactagac ctaggagctg tgctgggtaa ctcacatgag tcactggtca catcagcggc 27180 cgcagcttat agtaaagatt tttttgttaa taaaatgaaa gttaatattt atggatttat 27240 ttgaacatat ttacaagttt ttgttaataa atttatttac agaagtgtta aatttgaaaa 27300 tgtgttaaat gagagttaat atttatggat ataatattac tttatttaaa aataaaatga 27360 atttatatta aaatatgatt tggtaaaaga tattataaag aggaacttgg atgagaaaga 27420 cgacgatggg caaaagttgc atggcacacc gcttttgttc agtaacatct gcactgtggc 27480 gcctttttca cactcaccat gcatgctgcc acgtcagctt tcacactatg acaccactgg 27540 ccatacacgt tgccacctca gctttctcct cttctcacta tgacacgact ggccatgcat 27600 gcatgcatgc tgccacctca gctcccgcct cttcacccgt gtctttctct catgtcgcag 27660 tgctgcgtgc caacctcttc tctctataaa tagagagaag agaatgatgg ttaaactcaa 27720 accaaaacaa gaaaacatac acaaatagca aaacggatcc acaaccaaaa atgcttcagc 27780 acacttggct tccgaagcct cccaatctga ccctcttgtc agatgaggtt catctctgga 27840 ggattcctct tgaccagcct gagtcacaac ttcaagacct tgctgccacc ctgagcagtg 27900 atgaattggc gagggcaaac agattctact ttccagaaca cagaaggcgt ttcactgctg 27960 ggagaggcat cctcagatcc atcttgggtg gatacttggg agtggaaccg ggtcaagtca 28020 agtttgatta tgagtcccgt gggaaaccga tccttggtga cagatttgct gagagtggac 28080 tcctgttcaa cttgtctcac agccagaacc ttgccttgtg tgctgtcaac tacacgcgtc 28140 aaataggcat tgatcttgaa tatctgcgtc caacatctga cttggagtct cttgcaaaga 28200 ggttctttct cccaagagaa tatgaactct tgaggtcact ccctgatgag cagaaacaga 28260 agattttctt tcgttactgg acttgcaaag aggcttatct caaagcaacg ggagatggaa 28320 tagccaaact tgaagagatc gagatagcac tcaccccaac agaacctgcc aagctccaaa 28380 cagctcctgc gtggtctctg ttggagcttg tgccagatga caattgtgtt gcagctgtgg 28440 ctgttgcggg ttttggttgg cagcccaagt tctggcatta ctgagtagtt agcttaatca 28500 cttaggtcac ccagtccaag cccggcctag cacgatgatc atgagtgttg ctattgatgt 28560 atgttaatac tatacacagt catcgtgtgt gttttataaa actgtaagct taggatgttt 28620 gaggctaatg taattagcac tacaccgtaa taaatgagag gtttacttta tgttttaata 28680 atgttcttac ccttgctgct ctctaaactg taatggcgta cggttagcta aactaaagta 28740 aaaatcgatt cgcttgagta aaattagtga ccgggaaaga tcatcgacta ctactgctca 28800 gagatgtaat cggtaattaa gacttcaagc tcaataatta attaggagta aatctggctt 28860 cgaattttta aattgtcaac ttccaaagaa ggattggcaa gactcaatct caattgccca 28920 acaaacatat ggctctttac aaatttggaa tctctttttg atggcaagaa cgtcgtaatt 28980 cctctgtcga tctcatgtaa ttaccaaact catctactta ttactttctt tatatgcact 29040 tactgatgcg gccgcttaat taactactgt cactgaggcc gtagacgagt acggactgat 29100 ctaactagtg ctagcctcga ggtcgacacc cagctttctt gtacaaagtg gttgcggccg 29160 cttaattaaa tttaaattca attaatgcaa tcttgatttt caacaacgaa ggtaatggcg 29220 taaaagaaaa aatgtatgtt attgtattga tctttcatga tgttgaagcg tgccataata 29280 tgatgatgta taattaaaat attaactgtc gcattttatt gaaatggcac tgttatttca 29340 accatatctt tgattctgtt acatgacacg actgcaagaa gtaaataata gacgccgttg 29400 ttaaagaatt gctatcatat gtgcctaact agagggaatt tgagcgtcag acctaatcaa 29460 atattacaaa atatctcact ctgtcgccag caatggtgta atcagcgcag acaaatggcg 29520 taaagatcgc ggaaaaacct ccccgagtgg catgatagct gcctctgtat tgctgattta 29580 gtcagcctta tttgacttaa gggtgccctc gttagtgaca aattgctttc aaggagacag 29640 ccatgcccca cactttgttg aaaaacaaat tgcctttggg gagacggtaa agccagttgc 29700 tcttcaataa ggaatgtcga ggaggcaatg taaccgcctc tggtagtaca cttctctaat 29760 ccaaaaatca atttgtattc aagataccgc aaaaaactta tggtttaaac cctgcaggac 29820 tagtccagaa ggtaattatc caagatgtag catcaagaat ccaatgttta cgggaaaaac 29880 tatggaagta ttatgtaagc tcagcaagaa gcagatcaat atgcggcaca tatgcaacct 29940 atgttcaaaa atgaagaatg tacagataca agatcctata ctgccagaat acgaagaaga 30000 atacgtagaa attgaaaaag aagaaccagg cgaagaaaag aatcttgaag acgtaagcac 30060 tgacgacaac aatgaaaaga agaagataag gtcggtgatt gtgaaagaga catagaggac 30120 acatgtaagg tggaaaatgt aagggcggaa agtaacctta tcacaaagga atcttatccc 30180 ccactactta tccttttata tttttccgtg tcatttttgc ccttgagttt tcctatataa 30240 ggaaccaagt tcggcatttg tgaaaacaag aaaaaatttg gtgtaagcta ttttctttga 30300 agtactgagg atacaacttc agagaaattt gtaagtttgt aggtaccaga tctggatccc 30360 aaaccatgtc tccggagagg agaccagttg agattaggcc agctacagca gctgatatgg 30420 ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc tacagtgaac tttaggacag 30480 agccacaaac accacaagag tggattgatg atctagagag gttgcaagat agataccctt 30540 ggttggttgc tgaggttgag ggtgttgtgg ctggtattgc ttacgctggg ccctggaagg 30600 ctaggaacgc ttacgattgg acagttgaga gtactgttta cgtgtcacat aggcatcaaa 30660 ggttgggcct aggatctaca ttgtacacac atttgcttaa gtctatggag gcgcaaggtt 30720 ttaagtctgt ggttgctgtt ataggccttc caaacgatcc atctgttagg ttgcatgagg 30780 ctttgggata cacagcccgg ggtacattgc gcgcagctgg atacaagcat ggtggatggc 30840 atgatgttgg tttttggcaa agggattttg agttgccagc tcctccaagg ccagttaggc 30900 cagttaccca aatctgagta gttagcttaa tcacctagag ctcgatcggc ggcaatagct 30960 tcttagcgcc atcccgggtt gatcctatct gtgttgaaat agttgcggtg ggcaaggctc 31020 tctttcagaa agacaggcgg ccaaaggaac ccaaggtgag gtgggctatg gctctcagtt 31080 ccttgtggaa gcgcttggtc taaggtgcag aggtgttagc gggatgaagc aaaagtgtcc 31140 gattgtaaca agatatgttg atcctacgta aggatattaa agtatgtatt catcactaat 31200 ataatcagtg tattccaata tgtactacga tttccaatgt ctttattgtc gccgtatgta 31260 atcggcgtca caaaataatc cccggtgact ttcttttaat ccaggatgaa ataatatgtt 31320 attataattt ttgcgatttg gtccgttata ggaattgaag tgtgcttgag gtcggtcgcc 31380 accactccca tttcataatt ttacatgtat ttgaaaaata aaaatttatg gtattcaatt 31440 taaacacgta tacttgtaaa gaatgatatc ttgaaagaaa tatagtttaa atatttattg 31500 ataaaataac aagtcaggta ttatagtcca agcaaaaaca taaatttatt gatgcaagtt 31560 taaattcaga aatatttcaa taactgatta tatcagctgg tacattgccg tagatgaaag 31620 actgagtgcg atattatggt gtaatacata ggaattcgtt taaacgatct gcgtctaatt 31680 ttcggtccaa cttgcacagg aaagacgtcg accgcggtag ctcttgccca gcagactggg 31740 cttccagtcc tttcgctcga tcgggtccaa tgttgtcctc agctgtgaac cggaagcgga 31800 cgaccaacag tggaagaact gaaaggaacg agccgtctat accttgatga tcggcctctg 31860 gtgaagggta tcatcgcagc caagcaagct catgaaaggc tgatggggga ggtgtataat 31920 tatgaggccc acggcgggct tattctttag ggaggatcta tctcgttgct caagtgcatg 31980 gcgcaaagca gttattggag tgcggatttt cgttggcata ttattcgcca cgagttagca 32040 gacgaagaga ccttcatgaa cgtggccaag gccagagtta agcagatgtt acgccctgct 32100 gcaggccttt ctattatcca atagttggtt gatctttgga aagagcctcg gctgaggccc 32160 atactgaaag agatcgatgg atatcgatat gccatgttgt ttgctagcca gaaccagatc 32220 acatccgata tgctattgca gcttgacgca gatatggagg ataagttgat tcatgggatc 32280 gctcaggagt agctcatcca tgcacgccga caagaacaga aattccgtcg agttaacgca 32340 gccgcttacg acggattcga aggtcatcca ttcggaatgt attagtttgc accagctccg 32400 cgtcacacct gtcttcattt gaataagatg ttagcaattg tttttagctt tgtcttgttg 32460 tggcagggcg gcaagtgctt cagacatcat tctgttttca aattttatgc tggagaacag 32520 cttcttaatt cctttggaaa taatagactg cgtcttaaaa ttcagatgtc tggatataga 32580 tatgattgta aaataaccta tttaagtgtc atttagaaca taagttttat gaatgttctt 32640 ccattttcgt catcgaacga ataagagtaa atacaccttt tttaacatta caaataagtt 32700 cttatacgtt gtttatacac cgggaatcat ttccattatt ttcgcgcaaa agtcacggat 32760 attcgtgaaa gcgacataaa ctgcgaaatt tgcggggagt gtcttgagtt tgcctcgagg 32820 ctagcgcatg cacatagaca cacacatcat ctcattgatg cttggtaata attgtcatta 32880 gattgttttt atgcatagat gcactcgaaa tcagccaatt ttagacaagt atcaaacgga 32940 tgtgacttca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt 33000 tga 33003 <210> 37 <211> 33951 <212> DNA <213> Artificial sequence <220> <223> Plasmid pDAB7363 T-strand <400> 37 cagtcagcat catcacacca aaagttaggc ccgaatagtt tgaaattaga aagctcgcaa 60 ttgaggtcta caggccaaat tcgctcttag ccgtacaata ttactcaccg gatcctaacc 120 ggtttaatta aggcgcgcca tgcccgggca agcggccgca caagtttgta caaaaaagca 180 ggcttctaga cctaggtgga gtcatcacgc agactatctc agcatgtgcg tagcacgcgg 240 ccgcagctta tagtaaagat ttttttgtta ataaaatgaa agttaatatt tatggattta 300 tttgaacata tttacaagtt tttgttaata aatttattta cagaagtgtt aaatttgaaa 360 atgtgttaaa tgagagttaa tatttatgga tataatatta ctttatttaa aaataaaatg 420 aatttatatt aaaatatgat ttggtaaaag atattataaa gaggaacttg gatgagaaag 480 acgacgatgg gcaaaagttg catggcacac cgcttttgtt cagtaacatc tgcactgtgg 540 cgcctttttc acactcacca tgcatgctgc cacgtcagct ttcacactat gacaccactg 600 gccatacacg ttgccacctc agctttctcc tcttctcact atgacacgac tggccatgca 660 tgcatgcatg ctgccacctc agctcccgcc tcttcacccg tgtctttctc tcatgtcgca 720 gtgctgcgtg ccaacctctt ctctctataa atagagagaa gagaatgatg gttaaactca 780 aaccaaaaca agaaaacata cacaaatagc aaaacggatc cacaaccaaa aatggcttcc 840 tctatgctct cttccgctac tatggttgcc tctccggctc aggccactat ggtcgctcct 900 ttcaacggac ttaagtcctc cgctgccttc ccagccaccc gcaaggctaa caacgacatt 960 acttccatca caagcaacgg cggaagagtt aactgcatgc aggtgtggcc tccgattgga 1020 aagaagaagt ttgagactct ctcttacctt cctgacctta ccgattccga agcagcgagg 1080 ctgcaagagc agaaaggagg tgagatggac acgaggattg cgatcatagg catgagcgcc 1140 atacttcctt gtggaaccac ggtgagagag tcatgggaga ccataagggc tggcattgat 1200 tgcctgtctg atcttcctga ggatcgtgtt gatgtgactg cgtactttga cccagtcaag 1260 accacaaagg acaaaatcta ctgcaagaga ggaggtttca ttcctgagta tgactttgat 1320 gcgcgtgagt ttggtctcaa catgttccag atggaagatt ctgatgcgaa tcagaccatc 1380 tcacttctca aggtcaaaga agcacttcaa gatgctggga ttgatgcact tggaaaggag 1440 aagaaaaaca taggatgcgt gcttggcatt ggaggtggac agaagagttc acatgagttc 1500 tacagcagac tcaactatgt ggttgtggag aaagtcttga gaaagatggg gatgccagag 1560 gaagatgtca aagtggctgt ggagaagtac aaagcgaact tcccagaatg gagacttgac 1620 tctttccctg gattccttgg gaatgtcact gctggcagat gcaccaacac attcaacttg 1680 gatggcatga actgcgttgt ggatgctgca tgtgcaagct ccttgatagc agtcaaggtt 1740 gcgattgatg aactgctcta tggtgactgc gacatgatgg tcactggtgc aacctgcaca 1800 gacaactcca ttgggatgta catggctttc tcaaagacac ctgtgttcag cacggaccca 1860 agtgtgagag cgtatgatga gaaaacgaaa gggatgctca ttggtgaggg atcagccatg 1920 ttggtgctca aacgttatgc agatgcagtg agggatggtg atgagattca tgctgtgatt 1980 cgtggttgtg caagctcaag tgatggcaaa gcagctggca tctacactcc cacaatcagt 2040 ggtcaagagg aagccttgag acgtgcatac aatcgtgcct gcgttgaccc tgccactgtc 2100 actttggttg agggtcatgg gactggcact ccagttggag ataggattga gttgactgcg 2160 ttgaggaact tgtttgacaa agcgtatggt gaagggaaca cagagaaagt tgcagttgga 2220 agtatcaagt ccagtattgg tcatttgaag gctgttgctg gtcttgctgg aatgatcaag 2280 gtcatcatgg ctctcaaaca caagactttg cctggaacga tcaatgtgga caatccaccc 2340 aatctctatg acaacactcc catcaatgag tcatccctct acatcaacac gatgaacaga 2400 ccctggttcc ctccacctgg tgttccgaga cgtgctggaa tatccagctt tggctttgga 2460 ggtgccaact accatgcagt gttggaagag gctgaaccag agcacacgac agcgtacaga 2520 ctcaacaaac gtccacaacc ggtgttgatg atggctgcaa ctcctgctgc gttgcagagc 2580 ctttgtgaag ctcagctgaa agagtttgaa gctgcgatca aggagaatga gacagtcaag 2640 aatactgcct acatcaagtg tgtcaagttt ggagaacagt tcaagttccc tgggagcata 2700 cctgcgacca atgcgagact gggattcttg gtcaaagatg cggaggatgc gtgctccacg 2760 cttcgtgcta tatgtgctca gtttgccaaa gatgtgacca aagaagcgtg gaggcttccg 2820 agagaaggag tttctttccg tgccaaaggc attgccacca atggtgctgt tgctgcactc 2880 ttctctggac aaggtgctca gtacacacac atgttcagtg aagttgcgat gaactggcct 2940 cagttcagac agtccattgc tgcaatggac gctgcacagt ccaaggttgc tggaagtgac 3000 aaagactttg aaagagtctc tcaagtcctg tacccaagga aaccgtacga acgtgagcca 3060 gaacaagatc acaagaaaat ctcactcaca gcatacagcc agccgtcaac cctggcatgt 3120 gctcttggtg cctttgagat tttcaaggaa gctggattca ctccagactt tgcagctgga 3180 cacagcttgg gtgagtttgc tgcgctctat gcagctggtt gtgttgacag agatgagttg 3240 tttgaacttg tttgcagaag ggcaaggatc atgggaggca aagatgcacc tgccacaccc 3300 aaaggttgca tggcagctgt cataggaccc aacgcagaga acatcaaggt tcaagctgcc 3360 aacgtctggc ttggaaactc aaactctccg tctcagactg tcattactgg gtctgttgaa 3420 gggattcaag ctgaaagtgc cagattgcag aaggaagggt tcagagttgt gcctcttgct 3480 tgtgagtctg ccttccattc tccacagatg gaaaatgcca gctcagcttt caaagatgtc 3540 atatccaagg tttccttccg tacacccaaa gcagaaacaa agctcttcag caatgtttct 3600 ggagagacct atccgacgga tgccagagag atgctcactc aacacatgac ctcatctgtg 3660 aagttcctga cccaagtgag gaacatgcac caagctggtg cgagaatctt tgttgagttt 3720 ggaccgaaac aagttctcag caaacttgtt tctgaaaccc tcaaagatga cccgtctgtt 3780 gtcacagtgt ctgtgaaccc agcttctggc acggactctg acatacagtt gagggatgct 3840 gcagttcagc tggttgtggc tggtgtcaat cttcaagggt ttgacaagtg ggatgctcct 3900 gatgccacga ggatgcaagc catcaagaaa aagagaacca cactcagact ttcagctgcc 3960 acctatgtca gtgacaagac caagaaagtg agggatgctg ccatgaacga cggaagatgt 4020 gtcacatacc tcaagggtgc tgcaccactc atcaaggctc ctgaacctgt ggttgacgaa 4080 gctgccaaga gggaggctga aaggcttcag aaggagcttc aagacgctca gaggcagctt 4140 gacgacgcca aacgtgcagc tgcagaagcc aactccaaac ttgctgctgc caaggaggaa 4200 gccaagactg ctgcagcttc agccaagcct gcagttgaca ctgctgttgt ggagaaacat 4260 cgtgccatcc tcaagtccat gctggctgag ctggatggtt atggaagtgt tgatgcttct 4320 tcccttcagc aacagcaaca gcaacagact gctccggcgc ctgttaaagc cgctgcccct 4380 gccgctcccg ttgcaagtgc tcctgctccc gccgtgtcca acgagttgct tgagaaagct 4440 gagacagttg tgatggaggt tctcgctgcc aagactggat acgaaacaga catgattgag 4500 gcggacatgg agcttgagac tgagttggga attgacagca tcaagagggt tgaaattctt 4560 tctgaagtcc aagctatgct taacgttgag gcgaaagatg ttgatgctct ctctaggacg 4620 agaacagtgg gtgaagttgt caacgctatg aaggcagaaa tcgctggttc ttccgctccg 4680 gcgccggcag ctgccgcacc tgcaccggct aaagcggctc cagctgcagc tgcaccagct 4740 gtttctaatg agctgctcga aaaggcagaa actgttgtga tggaagtcct tgctgccaaa 4800 actggctacg aaactgacat gatagaatca gacatggagc ttgagacgga gcttggaatt 4860 gattccatca agcgtgtgga aatcctcagt gaagtccaag ccatgctcaa tgtggaagct 4920 aaggatgtgg atgctctctc aaggaccaga actgtcggtg aggttgtcaa tgccatgaag 4980 gctgagatag ctggaggctc cgctcctgcg ccggccgctg ccgctcctgg tcccgctgcc 5040 gcggctccag ctccagctgc agccgctccg gccgtctcaa acgaactcct tgaaaaagct 5100 gagaccgtcg tgatggaggt cttggcagct aaaaccggct atgagactga catgattgag 5160 tctgacatgg aattggagac agaacttggg atagacagca tcaaacgtgt tgaaatcctc 5220 agtgaggtcc aagctatgct gaacgtggaa gcgaaggatg ttgatgctct gtccagaacg 5280 aggaccgttg gtgaggttgt ggatgccatg aaggctgaaa ttgctggagg gtctgcgccc 5340 gcgccagctg cagccgctcc tgcacccgca gccgcggcac ctgctccagc tgcacctgca 5400 cctgccgttt cttcagaact cttggagaag gctgagacgg ttgtgatgga ggttttggct 5460 gcaaagactg gctatgagac cgacatgatt gaatctgata tggaattgga gacagagctt 5520 gggatcgatt ccatcaaaag agtggagatc ctgagtgaag tccaagccat gctcaatgtt 5580 gaagctaagg atgttgatgc actttcaaga acaaggacag tgggtgaggt tgtggatgcc 5640 atgaaggcgg agattgctgg agggagcgcg cctgcgccag ctgccgcggc acctgcaccg 5700 gcagccgcag ctccagctcc agctgcacca gcaccggccg cgcccgcgcc agctgtttca 5760 tccgaactcc tggaaaaggc cgagacggtt gtgatggagg tgctcgcagc taagactggt 5820 tacgagaccg acatgattga aagtgacatg gagcttgaaa ccgagttggg aatagatagc 5880 atcaaacgtg ttgaaatctt gtctgaggtc caagctatgt tgaacgtgga ggcaaaagat 5940 gtcgatgcgc tttcaagaac cagaacagtc ggtgaggtcg tggacgccat gaaggctgaa 6000 attgccggaa gctctgctag tgcgccagct gcagccgcac ctgcacctgc tgccgcggca 6060 cctgctccag ctgcagccgc tcctgccgtc agcaatgagt tgctggagaa agccgaaaca 6120 gttgtgatgg aagtccttgc tgcaaaaact ggttacgaaa cggacatgat tgagtcagac 6180 atggaattgg agaccgaact tggaatagat tccatcaaga gagtggagat cctctctgag 6240 gtgcaagcca tgctcaacgt tgaggctaaa gatgttgatg cactcagtag gacaagaact 6300 gtgggggaag ttgtcgatgc gatgaaggct gagattgctg gaggctctgc tcctgcgccg 6360 gcagctgccg cacctgcacc cgcagccgcg gcaccagctg tcagcaacga attgcttgag 6420 aaggctgaaa cagttgtcat ggaagtgctg gctgcaaaaa ccggctatga gactgacatg 6480 attgagtctg acatggaact tgagacggaa ttgggcattg acagcatcaa gcgtgttgag 6540 atcctcagtg aagtccaagc aatgctcaat gtggaggcta aggatgtgga tgccctctca 6600 aggaccagaa cagttggtga ggtcgttgat gcgatgaagg cggagatcgc tggaagttcc 6660 gcgcctgcgc cagctgcagc cgctcctgca cccgcagccg cggcaccggc tccagctgca 6720 gccgcaccgg ccgtctcctc agaactcctg gaaaaggctg agacagttgt gatggaagtc 6780 cttgctgcga aaactggcta tgagaccgac atgattgaat ctgacatgga actggagacc 6840 gagcttggaa ttgactccat caaacgtgtt gaaatcctct ctgaggttca agcgatgttg 6900 aatgtggagg ccaaagatgt tgatgctctt tccagaacaa ggacggtggg agaggtggtt 6960 gatgccatga aggctgagat agctgggggt tctgctcctg cgccagctgc agccgctcct 7020 gctcccgctg ccgcggcacc agctgtcagc aacgaactcc ttgaaaaagc tgaaactgtt 7080 gtgatggagg ttttggctgc aaagactgga tacgagactg acatgataga gtcagacatg 7140 gagttggaaa cagagcttgg gattgatagc atcaagcgtg tggaaatcct ttctgaggtt 7200 caagccatgc tgaacgttga ggccaaagat gtcgatgctt tgtcaaggac cagaacggtt 7260 ggagaagtgg tcgatgccat gaaggctgag atagctggtg gatcagcccc tgctccggct 7320 gcagccgctc ctgcaagtgc cggtgctgca ccagctgtga agattgattc agttcatggt 7380 gctgactgtg acgatttgtc actgatgcac gcaaaggtgg ttgacatcag aaggccagat 7440 gaactgattc tggaaagacc ggagaaccgt ccagttcttg tcgttgacga tggctcagag 7500 ttgacacttg ctcttgtgag ggtgttggga gcctgtgcag ttgtcttgac ctttgaggga 7560 ttgcagttgg ctcagagagc tggtgcagct gccattcgtc atgttctggc taaggacctt 7620 tcagcagaat cagctgagaa ggccatcaaa gaggctgagc aaagatttgg agcgttggga 7680 ggcttcataa gccagcaagc agaaaggttt gaaccagctg agatactggg tttcacactc 7740 atgtgtgcga agtttgccaa agcctctctc tgcactgctg tggctggagg tcgtcctgcc 7800 ttcatcggtg tggctaggct tgacggcaga cttggattca cctctcaagg cacttctgac 7860 gcactcaaga gagcacagcg tggagcgatc tttggactct gcaagacaat agggttggag 7920 tggtcagaat ctgatgtgtt cagccgtggt gtggacattg ctcaagggat gcacccagaa 7980 gatgctgcag ttgccatagt tagggagatg gcgtgtgctg acatcagaat cagagaggtt 8040 gggattggag caaaccagca aagatgcaca atcagagctg ccaagttgga gactgggaac 8100 cctcagagac agatagcgaa agacgatgtg cttctggttt ctggaggtgc tcgtgggatc 8160 acacctcttt gcatccgtga gatcacgcgt cagattgctg gaggcaagta cattctgttg 8220 ggtcgtagca aagtttctgc gtctgaacca gcatggtgtg ctggcatcac tgatgagaag 8280 gctgtgcaga aagctgccac ccaagagttg aagagggcct tcagtgctgg tgaaggaccc 8340 aaacccactc caagggctgt cacgaaactt gtgggatctg ttcttggtgc tcgtgaagtg 8400 agaagctcca ttgctgccat tgaagctctg ggtggcaaag cgatctacag ctcttgtgat 8460 gtgaactctg ctgcagatgt tgccaaagca gttagggatg cagagtccca gcttggtgcc 8520 agagttagtg gaatcgttca cgcttctggt gtgctccgtg atcgtctcat agagaagaaa 8580 ctccctgacg agtttgatgc tgtctttggg accaaagtca ctgggttgga gaatcttctg 8640 gctgcagttg atcgtgccaa cctcaaacac atggttctct tcagctccct tgctggtttc 8700 cacggcaatg ttggtcagtc tgactatgcg atggcaaacg aagctctcaa caagatgggt 8760 ttggaacttg cgaaggatgt cagtgtcaag tccatctgct ttggtccctg ggatggtggg 8820 atggtgacac ctcaactcaa gaaacagttt caagaaatgg gagttcagat cattcctcgt 8880 gaaggaggtg ctgatacagt tgcgaggatt gtgttgggat caagtcctgc tgagatcctt 8940 gttggaaact ggaggacacc atccaagaaa gttggaagtg acacgatcac cctgcataga 9000 aagatcagtg ccaagtccaa tcccttcttg gaggatcatg tcatacaagg cagaagggtg 9060 ttgccaatga cccttgctat tgggagcctt gctgagacct gccttggact cttccctggc 9120 tactctctct gggccataga tgacgcacag ctcttcaaag gtgtcactgt tgatggagac 9180 gtcaactgtg aagtgaccct cacaccgtcc actgctccga gtgggagggt gaatgtccaa 9240 gcgacgctca agacgttctc aagtggaaaa cttgttccag cctacagagc tgtcatagtg 9300 ttgtccaacc aaggtgctcc acctgccaat gcaaccatgc agcctccgag ccttgatgca 9360 gatcctgctt tgcaagggtc agtctatgat ggcaagactc tctttcatgg tcctgcgttc 9420 agaggcattg atgacgttct cagctgcacc aagagccagc ttgttgccaa gtgttctgct 9480 gttcctggat cagatgcagc cagaggagag tttgcaactg acactgatgc acatgatccc 9540 tttgtcaatg atcttgcctt tcaagcaatg ttggtctggg tgaggagaac gctgggacaa 9600 gctgcactcc cgaactccat tcagaggata gtccaacaca gaccggtccc acaagacaaa 9660 cccttctaca tcaccctcag atccaatcag agtggtggac atagccagca caaacatgca 9720 cttcagttcc acaacgaaca aggtgatctc ttcattgatg ttcaagcctc tgtcattgcg 9780 acagactcac ttgcgttctg agtagttagc ttaatcactt aggtcaccca gtccaagccc 9840 ggcctagcac gatgatcatg agtgttgcta ttgatgtatg ttaatactat acacagtcat 9900 cgtgtgtgtt ttataaaact gtaagcttag gatgtttgag gctaatgtaa ttagcactac 9960 accgtaataa atgagaggtt tactttatgt tttaataatg ttcttaccct tgctgctctc 10020 taaactgtaa tggcgtacgg ttagctaaac taaagtaaaa atcgattcgc ttgagtaaaa 10080 ttagtgaccg ggaaagatca tcgactacta ctgctcagag atgtaatcgg taattaagac 10140 ttcaagctca ataattaatt aggagtaaat ctggcttcga atttttaaat tgtcaacttc 10200 caaagaagga ttggcaagac tcaatctcaa ttgcccaaca aacatatggc tctttacaaa 10260 tttggaatct ctttttgatg gcaagaacgt cgtaattcct ctgtcgatct catgtaatta 10320 ccaaactcat ctacttatta ctttctttat atgcacttac tgatgcggcc gcttaattaa 10380 gatgagtgat actcaggact caggactcac tctgctgatc actagtgcta gcctcgaggt 10440 cgacacaact ttgtatacaa aagttgtcta gacctaggct cagcaactca tccagtcaag 10500 ttcgagtcag tgagttgggc ggccgcagct tatagtaaag atttttttgt taataaaatg 10560 aaagttaata tttatggatt tatttgaaca tatttacaag tttttgttaa taaatttatt 10620 tacagaagtg ttaaatttga aaatgtgtta aatgagagtt aatatttatg gatataatat 10680 tactttattt aaaaataaaa tgaatttata ttaaaatatg atttggtaaa agatattata 10740 aagaggaact tggatgagaa agacgacgat gggcaaaagt tgcatggcac accgcttttg 10800 ttcagtaaca tctgcactgt ggcgcctttt tcacactcac catgcatgct gccacgtcag 10860 ctttcacact atgacaccac tggccataca cgttgccacc tcagctttct cctcttctca 10920 ctatgacacg actggccatg catgcatgca tgctgccacc tcagctcccg cctcttcacc 10980 cgtgtctttc tctcatgtcg cagtgctgcg tgccaacctc ttctctctat aaatagagag 11040 aagagaatga tggttaaact caaaccaaaa caagaaaaca tacacaaata gcaaaacgga 11100 tccacaacca aaaatggctt cctctatgct ctcttccgct actatggttg cctctccggc 11160 tcaggccact atggtcgctc ctttcaacgg acttaagtcc tccgctgcct tcccagccac 11220 ccgcaaggct aacaacgaca ttacttccat cacaagcaac ggcggaagag ttaactgcat 11280 gcaggtgtgg cctccgattg gaaagaagaa gtttgagact ctctcttacc ttcctgacct 11340 taccgattcc gaagctgcaa ggaacgtgag tgcggctcat gagatgcatg acgagaagag 11400 gatagctgtt gtgggcatgg ctgttcagta tgctggatgc aaaacgaaag acgagttctg 11460 ggaagttctc atgaatggaa aagttgagag caaagtgatc agcgacaaac gtcttggttc 11520 aaattaccgt gctgaacact acaaagctga aaggagcaag tatgctgaca ccttctgcaa 11580 tgagacatat ggaactcttg atgagaacga aatagacaat gagcatgaac tccttttgaa 11640 ccttgccaaa caagccttgg ctgaaacatc tgtcaaagat tcaacaagat gtggcatagt 11700 ttctggatgc ctcagcttcc cgatggacaa cctccaaggt gaactcttga atgtgtacca 11760 gaaccatgtt gagaagaaac ttggtgccag agttttcaaa gatgcctctc actggtcaga 11820 aagagaacag tccaacaaac cagaggctgg tgacagaaga atcttcatgg accctgcttc 11880 ctttgttgct gaggaactca atcttggcgc tctccactac tctgttgatg ctgcctgtgc 11940 cactgcgctc tatgtgctca gactggcgca agatcacttg gtctctggtg cagctgatgt 12000 gatgctttgt ggtgcaacat gcctgccgga gccgtttttc atactctctg ggttctcaac 12060 cttccaagcc atgcctgttg gaacgggtca gaatgtgtcc atgccgctgc acaaagattc 12120 tcaaggattg acccctggtg aaggaggttc catcatggtc ctcaaacgtt tggatgacgc 12180 aatcagagat ggagatcaca tctatggaac acttttggga gccaatgtca gcaacagtgg 12240 cactggtctg ccacttaaac cccttttgcc gtcagagaaa aagtgcctca tggacaccta 12300 cacacgtatc aatgtgcatc cccacaagat tcagtatgtt gaatgccatg caactgggac 12360 tccacaaggt gatcgtgtgg aaattgatgc tgtgaaggct tgctttgaag gcaaagtccc 12420 acgttttgga accacaaaag ggaactttgg acacacattg gtggcagctg ggtttgctgg 12480 catgtgcaaa gtgcttctct ccatgaagca cgggatcatt ccacccactc cgggaataga 12540 tgacgaaacg aagatggacc ctctggttgt ctctggtgag gccattccct ggccagaaac 12600 gaatggagaa ccgaagcgtg ctggtctctc agcgtttgga tttggtggga ccaatgctca 12660 tgctgtcttt gaggaacatg acccaagcaa tgctgcatgc actggccatg actccatttc 12720 tgctctctct gcacgttgtg gtggtgagtc aaacatgaga atagcaatca ctgggatgga 12780 tgccactttt ggtgctttga aaggattgga tgcttttgaa agggccatct acactggtgc 12840 tcatggtgct ataccactcc cagaaaagag atggagattc ctggggaaag acaaggactt 12900 ccttgatctt tgtggagtca aagcaacacc gcatggctgt tacattgaag atgttgaagt 12960 tgatttccag aggctgagga ctccaatgac tcctgaagat atgctcttgc ctcaacagct 13020 tctggctgtc accacaattg atagggccat attggactct ggcatgaaga aaggtggaaa 13080 tgtggctgtc tttgtggggt tggggaccga tcttgaactc taccgtcacc gtgcaagggt 13140 tgctctcaag gagagagtga ggcctgaagc cagcaagaaa ctcaatgaca tgatgcagta 13200 catcaatgat tgtgggacaa gcacttctta caccagctac ataggcaact tggttgcaac 13260 aagggtcagt tcacaatggg gcttcacggg accttccttc acaatcacag aaggcaacaa 13320 ttctgtgtac agatgtgctg aacttggcaa atacctcttg gaaacgggag aggttgatgg 13380 ggttgtggtt gcgggtgttg atctctgtgg gagtgctgag aacctttacg tcaagtcaag 13440 gcgtttcaag gtgtctacat ctgacacccc gagagccagc tttgatgcag ctgctgatgg 13500 ttactttgtt ggagagggct gtggtgcatt tgtgctcaag cgtgagacaa gctgcaccaa 13560 ggatgacaga atctatgctt gcatggatgc catagttcct ggaaatgttc catctgcatg 13620 tttgagagag gcattggatc aagccagagt caagcctgga gacatcgaga tgttggagct 13680 ttctgctgat tcagcgaggc atttgaagga cccgtctgtt ctcccaaagg aactcactgc 13740 tgaggaagag attggcggac ttcagacgat tcttcgtgac gatgacaagc tcccaaggaa 13800 tgttgcaact ggaagtgtca aggccactgt tggtgacact ggttatgctt ctggtgctgc 13860 cagtttgatt aaggctgcgc tttgcatcta caaccgttac ctgcccagca atggagatga 13920 ctgggatgaa cctgcacctg aagcaccctg ggacagcaca ctctttgcgt gccagacctc 13980 aagagcttgg ttgaagaacc ctggtgagag acgttatgcg gctgtttccg gagtcagtga 14040 aaccagaagt tgctacagtg tgctgctttc tgaagcagag ggacactacg agagagagaa 14100 cagaatcagt ttggatgagg aagcacccaa gctcattgtg ttgagagcag actcccatga 14160 agagattctt ggaagattgg acaagataag ggaaaggttc ttgcagccca cgggtgctgc 14220 acccagagag tctgaattga aggcgcaagc ccgtaggatc tttcttgaac tccttggtga 14280 gacccttgcc caagatgcag cttcatctgg aagtcagaag ccccttgctc tcagcttggt 14340 ctccactcca tccaagctcc agagagaggt ggaacttgcg gcaaaaggca ttccgagatg 14400 tctcaagatg agacgtgatt ggagttcccc tgctggaagc agatatgcgc ctgagccttt 14460 ggccagtgac agagttgcct tcatgtatgg agaaggaagg agcccttact atggcatcac 14520 acaagacatt cataggattt ggcctgaact tcatgaggtc atcaatgaga aaacaaaccg 14580 tctctgggct gaaggtgaca gatgggtcat gccaagggcc agcttcaaga gtgagcttga 14640 gtcacaacag caagagtttg acagaaacat gattgaaatg ttccgtcttg gaatcctcac 14700 ctcaattgcc ttcacaaacc tggcgagaga tgttctcaac atcacgccca aagcagcctt 14760 tggactcagt ttgggagaga tttccatgat ctttgctttc tccaagaaaa acggtttgat 14820 aagcgatcag ttgacaaagg atttgagaga gtcagatgtc tggaacaaag ctctggctgt 14880 tgagttcaat gcgctcagag aagcctgggg cattccacag tctgtcccaa aagatgagtt 14940 ctggcaaggt tacatagtga ggggaacgaa acaagacata gaagcagcca tagcaccaga 15000 ctcaaagtat gtgaggttga ccatcatcaa tgatgccaac actgctctca tcagtggaaa 15060 gcctgatgca tgcaaagctg cgattgctcg tcttggtggc aacatccctg cgcttccagt 15120 cactcaaggc atgtgtgggc attgcccaga ggtgggacct tacaccaagg acatcgcaaa 15180 gatccacgcc aaccttgagt tccctgtggt tgatggtctt gatctctgga ctaccataaa 15240 ccagaagagg cttgtgccaa gagccacggg tgccaaagat gaatgggctc cttcatcctt 15300 tggggagtat gctggacagc tctatgagaa acaagccaac ttccctcaga tagttgaaac 15360 catctacaag cagaactatg atgtgtttgt tgaagttggg ccaaacaatc acagaagcac 15420 agcagtgaga accacgcttg gaccacagag aaaccacctt gctggtgcca tagacaagca 15480 gaacgaggat gcttggacga caatagtcaa gctcgtggct tcattgaagg ctcaccttgt 15540 tcctggagtg accatcagtc cgctctacca ctccaagctc gttgcagagg ctgaggcttg 15600 ttatgctgcg ctctgtaaag gtgagaaacc caagaaaaac aagtttgtga gaaagattca 15660 gttgaatggc agattcaaca gcaaggcaga ccccatctcc tcagcagacc ttgcatcctt 15720 cccacctgcg gaccctgcga ttgaagctgc catctcttca aggatcatga agcctgtggc 15780 tccgaaattc tatgcgcgtc tcaacattga tgagcaagat gagaccagag atccgatctt 15840 gaacaaggac aatgcgcctt ccagctccag ctcttccagc agttcctctt ccagcagttc 15900 atctccgtct cctgctcctt cagctccagt tcagaagaaa gctgccccag ctgcggagac 15960 aaaagctgtt gccagtgctg atgcacttcg tagtgccctg cttgatcttg actccatgct 16020 tgcgctcagc tctgcaagtg cttctggcaa ccttgttgag actgcgccat ctgatgccag 16080 tgtcattgtc cctccgtgca acattgctga tcttggcagc agagctttca tgaaaactta 16140 tggtgtttct gcgcctctgt acaccggtgc aatggccaag ggaattgcct ctgcggactt 16200 ggtgatcgca gctggaaggc aaggaatcct tgcgtcattt ggtgctggag gccttccaat 16260 gcaagttgtg cgtgagtcca ttgaaaagat tcaagctgcc ctgcccaatg gaccatatgc 16320 tgtcaacctc atccattctc cttttgacag caaccttgag aagggaaatg ttgatctctt 16380 cttggaaaag ggtgtcactt ttgttgaggc ttcagccttc atgacgttga caccgcaagt 16440 tgtcagatac agagcggctg ggttgacgag gaatgcagat gggtcagtca acataaggaa 16500 cagaatcatt gggaaagtct ccagaactga gctggctgaa atgttcatgc gtcctgcacc 16560 tgagcatctt ttgcagaagc tcattgcttc tggggagatc aatcaagaac aagctgagtt 16620 ggccagacgt gttcctgtgg ctgatgacat tgcggttgaa gctgattctg gtggacacac 16680 tgacaaccgt ccaatccatg tcattctgcc tctgattatc aacctgaggg ataggcttca 16740 cagagagtgt ggatacccag caaacctgag ggttcgtgtt ggtgctgggg gtggcattgg 16800 gtgtccccaa gctgcgctgg caactttcaa catgggtgcc tctttcattg tcactggaac 16860 agtgaaccaa gttgccaaac aatctgggac gtgtgacaat gtgaggaaac agcttgcgaa 16920 agcaacttac tcagatgttt gcatggcacc ggcagctgac atgtttgagg aaggtgtgaa 16980 acttcaagtt ttgaagaaag gaacaatgtt cccttcaaga gccaacaaac tctatgagtt 17040 gttctgcaag tatgattctt ttgagtccat gcctccagca gagcttgcga gggttgagaa 17100 aagaatcttc agtcgtgcat tggaagaggt ctgggatgag accaagaact tctacatcaa 17160 ccgtctccac aacccagaga agatacagag ggctgagagg gaccccaaac tgaagatgtc 17220 actgtgtttc agatggtatc tgtccctggc ttcaaggtgg gccaacactg gagcttctga 17280 cagagtcatg gactaccaag tttggtgtgg tcctgccatt ggttcattca atgatttcat 17340 caaaggaact taccttgatc cggcagtggc aaatgagtac ccgtgtgttg tgcagatcaa 17400 caaacagatt cttagaggag cgtgcttctt gaggcgtctt gaaatactga gaaatgcaag 17460 gctttcagat ggtgcagctg cacttgtggc gagcattgat gacacatatg tccctgctga 17520 gaagctgtga gtagttagct taatcactta ggtcacccag tccaagcccg gcctagcacg 17580 atgatcatga gtgttgctat tgatgtatgt taatactata cacagtcatc gtgtgtgttt 17640 tataaaactg taagcttagg atgtttgagg ctaatgtaat tagcactaca ccgtaataaa 17700 tgagaggttt actttatgtt ttaataatgt tcttaccctt gctgctctct aaactgtaat 17760 ggcgtacggt tagctaaact aaagtaaaaa tcgattcgct tgagtaaaat tagtgaccgg 17820 gaaagatcat cgactactac tgctcagaga tgtaatcggt aattaagact tcaagctcaa 17880 taattaatta ggagtaaatc tggcttcgaa tttttaaatt gtcaacttcc aaagaaggat 17940 tggcaagact caatctcaat tgcccaacaa acatatggct ctttacaaat ttggaatctc 18000 tttttgatgg caagaacgtc gtaattcctc tgtcgatctc atgtaattac caaactcatc 18060 tacttattac tttctttata tgcacttact gatgcggccg cttaattaat ctgacctgac 18120 cactagacct gagtgagtac agatgtgcta ctagtgctag cctcgaggtc gaccaacttt 18180 tctatacaaa gttgtctaga cctaggacta ggcactgagt gactcactga cctgactgag 18240 tgactggcgg ccgcagctta tagtaaagat ttttttgtta ataaaatgaa agttaatatt 18300 tatggattta tttgaacata tttacaagtt tttgttaata aatttattta cagaagtgtt 18360 aaatttgaaa atgtgttaaa tgagagttaa tatttatgga tataatatta ctttatttaa 18420 aaataaaatg aatttatatt aaaatatgat ttggtaaaag atattataaa gaggaacttg 18480 gatgagaaag acgacgatgg gcaaaagttg catggcacac cgcttttgtt cagtaacatc 18540 tgcactgtgg cgcctttttc acactcacca tgcatgctgc cacgtcagct ttcacactat 18600 gacaccactg gccatacacg ttgccacctc agctttctcc tcttctcact atgacacgac 18660 tggccatgca tgcatgcatg ctgccacctc agctcccgcc tcttcacccg tgtctttctc 18720 tcatgtcgca gtgctgcgtg ccaacctctt ctctctataa atagagagaa gagaatgatg 18780 gttaaactca aaccaaaaca agaaaacata cacaaatagc aaaacggatc cacaaccaaa 18840 aatggcttcc tctatgctct cttccgctac tatggttgcc tctccggctc aggccactat 18900 ggtcgctcct ttcaacggac ttaagtcctc cgctgccttc ccagccaccc gcaaggctaa 18960 caacgacatt acttccatca caagcaacgg cggaagagtt aactgcatgc aggtgtggcc 19020 tccgattgga aagaagaagt ttgagactct ctcttacctt cctgacctta ccgattccga 19080 agcgcttcgt gtcaagacca acaagaagcc atgttgggag atgaccaagg aggaactgac 19140 ctctggcaaa actgaggtgt tcaactatga ggaactcctg gagtttgcag agggtgacat 19200 agctaaggtt tttggtccgg agtttgctgt catagacaag taccccagaa gggtgaggct 19260 gcctgctcgt gaatacctgc ttgtgacaag ggtcacactc atggatgccg aggtcaacaa 19320 ctacagagtt ggtgccagaa tggtcactga gtatgatctt ccagtgaatg gcgaattgtc 19380 tgagggaggt gattgtccct gggcagttct ggttgagagt ggacagtgcg atttgatgct 19440 gatatcatac atgggaattg atttccagaa ccaaggtgac agagtttacc gtctgctcaa 19500 caccacgttg acattctatg gggttgccca tgagggagag acccttgagt atgacatcag 19560 agtgaccggt tttgccaaac gtttggatgg tggcatctcc atgttcttct ttgagtatga 19620 ctgctatgtc aatgggaggc ttctcattga gatgagagat ggctgtgctg gcttcttcac 19680 aaatgaggag cttgatgcgg gaaagggtgt ggtgttcaca agaggggatt tggctgccag 19740 agctaagatt ccaaagcaag atgtttctcc ctatgctgtc gctccctgcc ttcacaaaac 19800 aaaactcaac gaaaaggaga tgcagacctt ggttgacaaa gattgggctt ccgtttttgg 19860 ctcaaagaat ggcatgccgg agatcaacta caaactctgc gcacgtaaga tgctcatgat 19920 tgatagagtc accagcattg accataaggg aggtgtgtac ggtttgggtc agttggttgg 19980 tgagaaaatc ttggagaggg accattggta cttcccatgc cattttgtta aggatcaagt 20040 catggctgga tctcttgtca gcgacggttg ttcacaaatg ctcaagatgt acatgatctg 20100 gctcggtttg caccttacca ccggaccatt tgacttcaga cccgtcaacg gacacccgaa 20160 caaagttagg tgtaggggtc agatatctcc tcataaggga aagttggtct acgtcatgga 20220 gatcaaagag atgggattcg acgaggataa cgatccgtac gccattgctg acgtcaacat 20280 cattgacgtt gatttcgaaa agggtcaaga tttcagcctt gatagaatca gcgactacgg 20340 aaagggggat cttaacaaaa agatcgtcgt ggacttcaag ggcattgctc tcaagatgca 20400 gaagagatcc acgaacaaga acccctccaa agttcagccc gtttttgcta acggtgcagc 20460 cactgtggga ccggaagcgt ccaaggccag ctctggtgcc agtgctagtg cttctgctgc 20520 ccctgcgaag cctgctttct cagcagatgt tcttgcgcca aagccagttg cacttcctga 20580 acatatcctc aagggtgacg ctctcgctcc gaaagaaatg tcctggcatc ccatgtcaaa 20640 acttcctggc aacccgacgc cgttcttctc ccctagttct taccctccga gggcaatttg 20700 cttcatccct ttccctggca acccacttga caacaattgc aaagctggag aaatgcctct 20760 gaactggtac aacatgtcag agttcatgtg tggaaaagtt agcaactgct tgggaccaga 20820 gttcgcaaga tttgataagt caaatacgag cagaagccct gcttttgatt tggctcttgt 20880 gactagagtt gttgaagtca caaacatgga acatggcaag ttcctgaatg ttgattgcaa 20940 tcccagcaaa ggcacaatgg tgggggagtt tgactgtcct caagatgcgt ggttctttga 21000 tggttcttgc aatgatgggc acatgcctta ttccatcatc atggaaatcg gacttcagac 21060 atccggtgtt ctcaccagtg tgttgaaagc accactgacg atggacaaag atgacattct 21120 ctttaggaac cttgatgcaa gtgctgaaat ggtgaggcca gatgtggatg tgagggggaa 21180 aaccataagg aatgtgacca agtgcactgg atatgcaatg ttgggaaaga tggggattca 21240 cagattcacg tttgagttgt ctgttgatgg cgtggttttc tacaaaggaa gcacttcctt 21300 tggatggttc actccagagg tgtttgctca gcaagctgga cttgacaatg ggaaaaagac 21360 ggagccttgg tgcaaaacta acaacacgag tgtgagaaga gttgaaatcg catcagccaa 21420 aggcaaagaa cagctgactg agaaacttcc agatgcaact aatgctcaag ttctcagaag 21480 gtcagaacag tgtgagtatt tggattacct caacattgct cctgattctg ggctgcatgg 21540 gaaaggatat gcgcatggac acaaagatgt taacccacaa gactggttct tctcttgtca 21600 cttttggttt gatcctgtga tgcctggatc tttgggaatt gagtcaatgt tccagctcat 21660 tgaggcattt gcggtggacc agaacatacc gggagaatac aatgtgagca atccgacctt 21720 tgcccatgcc cctggaaaaa cggcgtggaa gtaccgtggt caattgacac caaagaaccg 21780 tgcgatggac tgtgaggtgc acatagtttc aatcacagcc tctcctgaaa atggaggcta 21840 cgttgacata gtggcagatg gagcactttg ggttgatggt ctccgtgtgt atgaagccaa 21900 agaactcaga gttcgtgttg tcagtgcaaa acctcaagcc attccggatg ttcaacaaca 21960 gccacccagc gcaaaagcgg accctgggaa aactggagtt gcactcagtc cgactcagtt 22020 gagagatgtc ctgcttgaag tggacaatcc attgtatctt ggtgttgaaa actccaattt 22080 ggtgcagttt gaatccaaac ctgcaacttc ttcaaggatt gtcagtatca aaccatgctc 22140 cataagtgat cttggtgaca agtccttcat ggaaacgtac aatgtgtcag cacctctgta 22200 cactggagca atggccaagg gaattgcgtc tgcggacctt gtcattgctg ctgggaagag 22260 gaagatattg ggttcttttg gagcgggtgg gcttccaatg caccatgtga gggcagcttt 22320 ggagaagata caagcggctc tgcctcaagg accatatgct gtgaacctca ttcattcacc 22380 ttttgacagc aacttggaaa aggggaatgt tgatctcttc ctcgaaaagg gcgttactgt 22440 ggtggaggct tctgcattca tgaccctcac tccccaagtt gttcgttacc gtgcagccgg 22500 actctcaagg aacgctgatg ggagtgtcaa catcagaaac cgtatcattg gaaaagtgtc 22560 cagaacagaa ctcgccgaaa tgttcatcag accggctccg gaacacctcc ttgagaaact 22620 catagcttct ggtgagataa cacaagaaca agccgaactt gcccgtcgtg ttcctgtcgc 22680 agacgacatc gctgttgaag ctgattctgg tggtcacact gacaaccgtc ccatccacgt 22740 catcttgcct ttgatcatca accttaggaa cagactgcac agagagtgtg gttatcctgc 22800 gcaccttaga gtcagagttg gagctggagg cggtgttggc tgcccacaag ctgctgcggc 22860 tgccctcaca atgggagctg ccttcatagt gactgggact gtcaatcaag ttgctaaaca 22920 gtccgggaca tgtgacaacg tgagaaaaca actcagtcaa gccacctaca gtgacatttg 22980 catggctcca gcagctgata tgtttgagga aggtgtgaaa ctccaagttc tcaagaaggg 23040 aacaatgttc ccatcaagag ctaacaaatt gtacgaactc ttttgcaagt acgactcttt 23100 cgattcaatg cctcctgctg aacttgaaag gatcgagaag aggatcttca agagggcact 23160 tcaagaggtc tgggaagaga ccaaggactt ttacatcaat ggtttgaaga atccagagaa 23220 gatacagaga gcggaacatg accccaaact caagatgtct ttgtgtttca gatggtatct 23280 tggtcttgca tcacgttggg ccaacatggg tgctcctgat cgtgtcatgg actatcaagt 23340 ctggtgtggt ccagccattg gagccttcaa tgacttcatc aaaggaacat atcttgatcc 23400 tgctgtctct aatgagtacc catgtgttgt ccagatcaac cttcagattt tgcgtggtgc 23460 atgttacctg aggcgtctta atgctctgag aaatgatccg aggattgatc ttgagactga 23520 agatgctgcc tttgtctatg agccaacgaa tgcgctttga gtagttagct taatcactta 23580 ggtcacccag tccaagcccg gcctagcacg atgatcatga gtgttgctat tgatgtatgt 23640 taatactata cacagtcatc gtgtgtgttt tataaaactg taagcttagg atgtttgagg 23700 ctaatgtaat tagcactaca ccgtaataaa tgagaggttt actttatgtt ttaataatgt 23760 tcttaccctt gctgctctct aaactgtaat ggcgtacggt tagctaaact aaagtaaaaa 23820 tcgattcgct tgagtaaaat tagtgaccgg gaaagatcat cgactactac tgctcagaga 23880 tgtaatcggt aattaagact tcaagctcaa taattaatta ggagtaaatc tggcttcgaa 23940 tttttaaatt gtcaacttcc aaagaaggat tggcaagact caatctcaat tgcccaacaa 24000 acatatggct ctttacaaat ttggaatctc tttttgatgg caagaacgtc gtaattcctc 24060 tgtcgatctc atgtaattac caaactcatc tacttattac tttctttata tgcacttact 24120 gatgcggccg cttaattaat cacgagtgga tgactgtcac gactgactca tgactgacta 24180 ctagtgctag cctcgaggtc gacacaactt tgtataataa agttgtctag acctaggcga 24240 gtcatgagtc gtgacatgac gtcatgctca tcagactgcg gccgcagctt atagtaaaga 24300 tttttttgtt aataaaatga aagttaatat ttatggattt atttgaacat atttacaagt 24360 ttttgttaat aaatttattt acagaagtgt taaatttgaa aatgtgttaa atgagagtta 24420 atatttatgg atataatatt actttattta aaaataaaat gaatttatat taaaatatga 24480 tttggtaaaa gatattataa agaggaactt ggatgagaaa gacgacgatg ggcaaaagtt 24540 gcatggcaca ccgcttttgt tcagtaacat ctgcactgtg gcgccttttt cacactcacc 24600 atgcatgctg ccacgtcagc tttcacacta tgacaccact ggccatacac gttgccacct 24660 cagctttctc ctcttctcac tatgacacga ctggccatgc atgcatgcat gctgccacct 24720 cagctcccgc ctcttcaccc gtgtctttct ctcatgtcgc agtgctgcgt gccaacctct 24780 tctctctata aatagagaga agagaatgat ggttaaactc aaaccaaaac aagaaaacat 24840 acacaaatag caaaacggat ccacaaccaa aaatgcccac tccagatgcc actgcgcctc 24900 tcaacaagcc gtcagactat gctgtgtacc acgaagagga tggtcctttc tggactgctg 24960 attcctctgg tgtcagcaga gtgaacttct ctgaaactgg tgtgggatct gaaggtgtca 25020 ttcctgcgct cacactgatt gatgtgtttg agcgtgcggt caaacgtggt ggcaatagga 25080 ttgcattcag aacagagaac atgccaacct tgaggcgtgg agaagaggct cctgatgctt 25140 tgccgctcaa agattggaag tcctggtctt ggaaacagta caaagctgat gttcacagaa 25200 ttgcgagggc tctgatggat ttgggagttg agcagcacga tgcggtctcc atctttggct 25260 tcaactcacc agaatggttc ctcagtgctg tcggtgcagt tcatgctggt gccaagatcg 25320 ctggaatcta cccttcagac actcctgctc aagtgcagta caaagccttc cactctgaca 25380 ctgctgttgc agttgtggag aacgaacagt gcttcaagaa attcgcagag gtggttgagg 25440 atctccctta cctcaaagcc attgtttgtt gggattatga agccacagac atcacaaggg 25500 aagatggttc agtggttgaa gtcctgacct ttgctgagtt cctgaagaga ggtgacactg 25560 tggaagcagc tgcacttgat gagagaatca gcaagattga gcccaccatg tgtgctgccc 25620 tgatctacac cagtggcaca actgggagac ccaaagctgt catgatctcc catgacaacc 25680 ttgtctttga agcatctgct gtggtcccga acttgggagg ggcatgcacg accactgctg 25740 aggaaaggat tctttcctac cttcctttgt ctcatgttgc tggaatgatg gttgacatca 25800 ttgctccaat catagcgaca gcgttccaca aaggaaggat ctgtgtttgt tttgccagac 25860 cgtatgattt gagaactgga acccttgggc aaagactcaa tgcagtggag ccaaccatat 25920 tccttggtgt gccacgtgtg tgggaaaaga tccaagagaa attgatggct gttggtgcca 25980 aaaccactgg tctcaagaag aaactcagca cggcagccaa gaaacgtggg cttgaatttc 26040 aagaggaaca acagataggg agaagtggtg ccaaccctgg ctttggaccg cttggaatct 26100 acaagaaact tttgggcctc atcaaaggaa agttggggct gaccaaatgc aagtttgcct 26160 ttgctggcgc agctccaatg acccgtgaaa cccttcagta ctttggagcg ctgaacatca 26220 acatcaatga ggtctatgga atgtctgaat gcagtggggc tgccacctgg tcaactgaca 26280 aagctcatga gtggggcact gttggctatg agatgccctc ctgtgaagtc agagtgttca 26340 agattgcgga ggatggcacc aagacagagt gcccaagagc tgctgacatc atgcatgcca 26400 cagaagagga acaaggtgaa gtttgcttcc gtggaaggaa catcatgatg ggatacttgg 26460 ccaacccgaa acttggtgat gaccatgttg ctgagattga agagaagaat gcagctgcca 26520 tagatagcga agggtggctt cactctggtg acaagggagc aatttccaca aggggaatgc 26580 tcaagataac gggcagatac aaggagctta tcataggtgc tggtggggag aatgtggctc 26640 ctgttcccat tgaagatgcc atcaaggcga ggatgccttt tgtttcaaat gcaatgatgg 26700 ttggagacaa gaggaagttc atggctgtcc ttctgacttt gaaaacggtt ggagcaactg 26760 gagagcttcc tggcacaaac aagctcatgg gagcagctgc ggactatggt gagaccattg 26820 aggatgcctg tgacaatgag gcgctcattg aagagataac gcagcaactc aaagagatag 26880 gtgacgatgg agatgtcacg ccaagcaatg cagcccgtat acaaaagttc acgattttgc 26940 cactcgactt cagtgtctcc acagatgaac tgacagcaac gttgaaattg aagagaagtg 27000 tggttgcaga caagtatgag gacatcatag aggctttcta tgagagcaag tctgtctttg 27060 tgccttactc aacagttgga gcttatgcaa cgggtggacc agttgatgac tctgttgtgg 27120 atggatcatt caaaggtgac tttagcatga ttggggatga cgatccggat cttcagaatg 27180 tggatgtcct tgaagcaatt gatgaggaca actgagtagt tagcttaatc acttaggtca 27240 cccagtccaa gcccggccta gcacgatgat catgagtgtt gctattgatg tatgttaata 27300 ctatacacag tcatcgtgtg tgttttataa aactgtaagc ttaggatgtt tgaggctaat 27360 gtaattagca ctacaccgta ataaatgaga ggtttacttt atgttttaat aatgttctta 27420 cccttgctgc tctctaaact gtaatggcgt acggttagct aaactaaagt aaaaatcgat 27480 tcgcttgagt aaaattagtg accgggaaag atcatcgact actactgctc agagatgtaa 27540 tcggtaatta agacttcaag ctcaataatt aattaggagt aaatctggct tcgaattttt 27600 aaattgtcaa cttccaaaga aggattggca agactcaatc tcaattgccc aacaaacata 27660 tggctcttta caaatttgga atctcttttt gatggcaaga acgtcgtaat tcctctgtcg 27720 atctcatgta attaccaaac tcatctactt attactttct ttatatgcac ttactgatgc 27780 ggccgcttaa ttaatctgat catggatgct acgtcacggc agtacaggac tatcactaga 27840 cctaggagct gtgctgggta actcacatga gtcactggtc acatcagcgg ccgcagctta 27900 tagtaaagat ttttttgtta ataaaatgaa agttaatatt tatggattta tttgaacata 27960 tttacaagtt tttgttaata aatttattta cagaagtgtt aaatttgaaa atgtgttaaa 28020 tgagagttaa tatttatgga tataatatta ctttatttaa aaataaaatg aatttatatt 28080 aaaatatgat ttggtaaaag atattataaa gaggaacttg gatgagaaag acgacgatgg 28140 gcaaaagttg catggcacac cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc 28200 acactcacca tgcatgctgc cacgtcagct ttcacactat gacaccactg gccatacacg 28260 ttgccacctc agctttctcc tcttctcact atgacacgac tggccatgca tgcatgcatg 28320 ctgccacctc agctcccgcc tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg 28380 ccaacctctt ctctctataa atagagagaa gagaatgatg gttaaactca aaccaaaaca 28440 agaaaacata cacaaatagc aaaacggatc cacaaccaaa aatggcttcc tctatgctct 28500 cttccgctac tatggttgcc tctccggctc aggccactat ggtcgctcct ttcaacggac 28560 ttaagtcctc cgctgccttc ccagccaccc gcaaggctaa caacgacatt acttccatca 28620 caagcaacgg cggaagagtt aactgcatgc aggtgtggcc tccgattgga aagaagaagt 28680 ttgagactct ctcttacctt cctgacctta ccgattccga acttcagcac acttggcttc 28740 cgaagcctcc caatctgacc ctcttgtcag atgaggttca tctctggagg attcctcttg 28800 accagcctga gtcacaactt caagaccttg ctgccaccct gagcagtgat gaattggcga 28860 gggcaaacag attctacttt ccagaacaca gaaggcgttt cactgctggg agaggcatcc 28920 tcagatccat cttgggtgga tacttgggag tggaaccggg tcaagtcaag tttgattatg 28980 agtcccgtgg gaaaccgatc cttggtgaca gatttgctga gagtggactc ctgttcaact 29040 tgtctcacag ccagaacctt gccttgtgtg ctgtcaacta cacgcgtcaa ataggcattg 29100 atcttgaata tctgcgtcca acatctgact tggagtctct tgcaaagagg ttctttctcc 29160 caagagaata tgaactcttg aggtcactcc ctgatgagca gaaacagaag attttctttc 29220 gttactggac ttgcaaagag gcttatctca aagcaacggg agatggaata gccaaacttg 29280 aagagatcga gatagcactc accccaacag aacctgccaa gctccaaaca gctcctgcgt 29340 ggtctctgtt ggagcttgtg ccagatgaca attgtgttgc agctgtggct gttgcgggtt 29400 ttggttggca gcccaagttc tggcattact gagtagttag cttaatcact taggtcaccc 29460 agtccaagcc cggcctagca cgatgatcat gagtgttgct attgatgtat gttaatacta 29520 tacacagtca tcgtgtgtgt tttataaaac tgtaagctta ggatgtttga ggctaatgta 29580 attagcacta caccgtaata aatgagaggt ttactttatg ttttaataat gttcttaccc 29640 ttgctgctct ctaaactgta atggcgtacg gttagctaaa ctaaagtaaa aatcgattcg 29700 cttgagtaaa attagtgacc gggaaagatc atcgactact actgctcaga gatgtaatcg 29760 gtaattaaga cttcaagctc aataattaat taggagtaaa tctggcttcg aatttttaaa 29820 ttgtcaactt ccaaagaagg attggcaaga ctcaatctca attgcccaac aaacatatgg 29880 ctctttacaa atttggaatc tctttttgat ggcaagaacg tcgtaattcc tctgtcgatc 29940 tcatgtaatt accaaactca tctacttatt actttcttta tatgcactta ctgatgcggc 30000 cgcttaatta actactgtca ctgaggccgt agacgagtac ggactgatct aactagtgct 30060 agcctcgagg tcgacaccca gctttcttgt acaaagtggt tgcggccgct taattaaatt 30120 taaattcaat taatgcaatc ttgattttca acaacgaagg taatggcgta aaagaaaaaa 30180 tgtatgttat tgtattgatc tttcatgatg ttgaagcgtg ccataatatg atgatgtata 30240 attaaaatat taactgtcgc attttattga aatggcactg ttatttcaac catatctttg 30300 attctgttac atgacacgac tgcaagaagt aaataataga cgccgttgtt aaagaattgc 30360 tatcatatgt gcctaactag agggaatttg agcgtcagac ctaatcaaat attacaaaat 30420 atctcactct gtcgccagca atggtgtaat cagcgcagac aaatggcgta aagatcgcgg 30480 aaaaacctcc ccgagtggca tgatagctgc ctctgtattg ctgatttagt cagccttatt 30540 tgacttaagg gtgccctcgt tagtgacaaa ttgctttcaa ggagacagcc atgccccaca 30600 ctttgttgaa aaacaaattg cctttgggga gacggtaaag ccagttgctc ttcaataagg 30660 aatgtcgagg aggcaatgta accgcctctg gtagtacact tctctaatcc aaaaatcaat 30720 ttgtattcaa gataccgcaa aaaacttatg gtttaaaccc tgcaggacta gtccagaagg 30780 taattatcca agatgtagca tcaagaatcc aatgtttacg ggaaaaacta tggaagtatt 30840 atgtaagctc agcaagaagc agatcaatat gcggcacata tgcaacctat gttcaaaaat 30900 gaagaatgta cagatacaag atcctatact gccagaatac gaagaagaat acgtagaaat 30960 tgaaaaagaa gaaccaggcg aagaaaagaa tcttgaagac gtaagcactg acgacaacaa 31020 tgaaaagaag aagataaggt cggtgattgt gaaagagaca tagaggacac atgtaaggtg 31080 gaaaatgtaa gggcggaaag taaccttatc acaaaggaat cttatccccc actacttatc 31140 cttttatatt tttccgtgtc atttttgccc ttgagttttc ctatataagg aaccaagttc 31200 ggcatttgtg aaaacaagaa aaaatttggt gtaagctatt ttctttgaag tactgaggat 31260 acaacttcag agaaatttgt aagtttgtag gtaccagatc tggatcccaa accatgtctc 31320 cggagaggag accagttgag attaggccag ctacagcagc tgatatggcc gcggtttgtg 31380 atatcgttaa ccattacatt gagacgtcta cagtgaactt taggacagag ccacaaacac 31440 cacaagagtg gattgatgat ctagagaggt tgcaagatag atacccttgg ttggttgctg 31500 aggttgaggg tgttgtggct ggtattgctt acgctgggcc ctggaaggct aggaacgctt 31560 acgattggac agttgagagt actgtttacg tgtcacatag gcatcaaagg ttgggcctag 31620 gatctacatt gtacacacat ttgcttaagt ctatggaggc gcaaggtttt aagtctgtgg 31680 ttgctgttat aggccttcca aacgatccat ctgttaggtt gcatgaggct ttgggataca 31740 cagcccgggg tacattgcgc gcagctggat acaagcatgg tggatggcat gatgttggtt 31800 tttggcaaag ggattttgag ttgccagctc ctccaaggcc agttaggcca gttacccaaa 31860 tctgagtagt tagcttaatc acctagagct cgatcggcgg caatagcttc ttagcgccat 31920 cccgggttga tcctatctgt gttgaaatag ttgcggtggg caaggctctc tttcagaaag 31980 acaggcggcc aaaggaaccc aaggtgaggt gggctatggc tctcagttcc ttgtggaagc 32040 gcttggtcta aggtgcagag gtgttagcgg gatgaagcaa aagtgtccga ttgtaacaag 32100 atatgttgat cctacgtaag gatattaaag tatgtattca tcactaatat aatcagtgta 32160 ttccaatatg tactacgatt tccaatgtct ttattgtcgc cgtatgtaat cggcgtcaca 32220 aaataatccc cggtgacttt cttttaatcc aggatgaaat aatatgttat tataattttt 32280 gcgatttggt ccgttatagg aattgaagtg tgcttgaggt cggtcgccac cactcccatt 32340 tcataatttt acatgtattt gaaaaataaa aatttatggt attcaattta aacacgtata 32400 cttgtaaaga atgatatctt gaaagaaata tagtttaaat atttattgat aaaataacaa 32460 gtcaggtatt atagtccaag caaaaacata aatttattga tgcaagttta aattcagaaa 32520 tatttcaata actgattata tcagctggta cattgccgta gatgaaagac tgagtgcgat 32580 attatggtgt aatacatagg aattcgttta aacgatctgc gtctaatttt cggtccaact 32640 tgcacaggaa agacgtcgac cgcggtagct cttgcccagc agactgggct tccagtcctt 32700 tcgctcgatc gggtccaatg ttgtcctcag ctgtgaaccg gaagcggacg accaacagtg 32760 gaagaactga aaggaacgag ccgtctatac cttgatgatc ggcctctggt gaagggtatc 32820 atcgcagcca agcaagctca tgaaaggctg atgggggagg tgtataatta tgaggcccac 32880 ggcgggctta ttctttaggg aggatctatc tcgttgctca agtgcatggc gcaaagcagt 32940 tattggagtg cggattttcg ttggcatatt attcgccacg agttagcaga cgaagagacc 33000 ttcatgaacg tggccaaggc cagagttaag cagatgttac gccctgctgc aggcctttct 33060 attatccaat agttggttga tctttggaaa gagcctcggc tgaggcccat actgaaagag 33120 atcgatggat atcgatatgc catgttgttt gctagccaga accagatcac atccgatatg 33180 ctattgcagc ttgacgcaga tatggaggat aagttgattc atgggatcgc tcaggagtag 33240 ctcatccatg cacgccgaca agaacagaaa ttccgtcgag ttaacgcagc cgcttacgac 33300 ggattcgaag gtcatccatt cggaatgtat tagtttgcac cagctccgcg tcacacctgt 33360 cttcatttga ataagatgtt agcaattgtt tttagctttg tcttgttgtg gcagggcggc 33420 aagtgcttca gacatcattc tgttttcaaa ttttatgctg gagaacagct tcttaattcc 33480 tttggaaata atagactgcg tcttaaaatt cagatgtctg gatatagata tgattgtaaa 33540 ataacctatt taagtgtcat ttagaacata agttttatga atgttcttcc attttcgtca 33600 tcgaacgaat aagagtaaat acaccttttt taacattaca aataagttct tatacgttgt 33660 ttatacaccg ggaatcattt ccattatttt cgcgcaaaag tcacggatat tcgtgaaagc 33720 gacataaact gcgaaatttg cggggagtgt cttgagtttg cctcgaggct agcgcatgca 33780 catagacaca cacatcatct cattgatgct tggtaataat tgtcattaga ttgtttttat 33840 gcatagatgc actcgaaatc agccaatttt agacaagtat caaacggatg tgacttcagt 33900 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg a 33951 <210> 38 <211> 105 <212> DNA <213> Artificial sequence <220> <223> Acyl-ACP Thioesterase Transit Peptide <400> 38 atgcttaagc tctcttgtaa cgttaccaat catcttcaca cattctcatt ctttagcgat 60 tctagtctgt tcatccctgt caacagaagg actcttgctg tgtct 105 <210> 39 <211> 38811 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7365 <400> 39 cttgtacaaa gtggttgcgg ccgcttaatt aaatttaaat tcaattaatg caatcttgat 60 tttcaacaac gaaggtaatg gcgtaaaaga aaaaatgtat gttattgtat tgatctttca 120 tgatgttgaa gcgtgccata atatgatgat gtataattaa aatattaact gtcgcatttt 180 attgaaatgg cactgttatt tcaaccatat ctttgattct gttacatgac acgactgcaa 240 gaagtaaata atagacgccg ttgttaaaga attgctatca tatgtgccta actagaggga 300 atttgagcgt cagacctaat caaatattac aaaatatctc actctgtcgc cagcaatggt 360 gtaatcagcg cagacaaatg gcgtaaagat cgcggaaaaa cctccccgag tggcatgata 420 gctgcctctg tattgctgat ttagtcagcc ttatttgact taagggtgcc ctcgttagtg 480 acaaattgct ttcaaggaga cagccatgcc ccacactttg ttgaaaaaca aattgccttt 540 ggggagacgg taaagccagt tgctcttcaa taaggaatgt cgaggaggca atgtaaccgc 600 ctctggtagt acacttctct aatccaaaaa tcaatttgta ttcaagatac cgcaaaaaac 660 ttatggttta aaccctgcag gactagtcca gaaggtaatt atccaagatg tagcatcaag 720 aatccaatgt ttacgggaaa aactatggaa gtattatgta agctcagcaa gaagcagatc 780 aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat acaagatcct 840 atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc aggcgaagaa 900 aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat aaggtcggtg 960 attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg gaaagtaacc 1020 ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc gtgtcatttt 1080 tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac aagaaaaaat 1140 ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa tttgtaagtt 1200 tgtaggtacc agatctggat cccaaaccat gtctccggag aggagaccag ttgagattag 1260 gccagctaca gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac 1320 gtctacagtg aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga 1380 gaggttgcaa gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat 1440 tgcttacgct gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt 1500 ttacgtgtca cataggcatc aaaggttggg cctaggatct acattgtaca cacatttgct 1560 taagtctatg gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga 1620 tccatctgtt aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc 1680 tggatacaag catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc 1740 agctcctcca aggccagtta ggccagttac ccaaatctga gtagttagct taatcaccta 1800 gagctcgatc ggcggcaata gcttcttagc gccatcccgg gttgatccta tctgtgttga 1860 aatagttgcg gtgggcaagg ctctctttca gaaagacagg cggccaaagg aacccaaggt 1920 gaggtgggct atggctctca gttccttgtg gaagcgcttg gtctaaggtg cagaggtgtt 1980 agcgggatga agcaaaagtg tccgattgta acaagatatg ttgatcctac gtaaggatat 2040 taaagtatgt attcatcact aatataatca gtgtattcca atatgtacta cgatttccaa 2100 tgtctttatt gtcgccgtat gtaatcggcg tcacaaaata atccccggtg actttctttt 2160 aatccaggat gaaataatat gttattataa tttttgcgat ttggtccgtt ataggaattg 2220 aagtgtgctt gaggtcggtc gccaccactc ccatttcata attttacatg tatttgaaaa 2280 ataaaaattt atggtattca atttaaacac gtatacttgt aaagaatgat atcttgaaag 2340 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa 2400 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc 2460 tggtacattg ccgtagatga aagactgagt gcgatattat ggtgtaatac ataggaattc 2520 gtttaaacga tctgcgtcta attttcggtc caacttgcac aggaaagacg tcgaccgcgg 2580 tagctcttgc ccagcagact gggcttccag tcctttcgct cgatcgggtc caatgttgtc 2640 ctcagctgtg aaccggaagc ggacgaccaa cagtggaaga actgaaagga acgagccgtc 2700 tataccttga tgatcggcct ctggtgaagg gtatcatcgc agccaagcaa gctcatgaaa 2760 ggctgatggg ggaggtgtat aattatgagg cccacggcgg gcttattctt tagggaggat 2820 ctatctcgtt gctcaagtgc atggcgcaaa gcagttattg gagtgcggat tttcgttggc 2880 atattattcg ccacgagtta gcagacgaag agaccttcat gaacgtggcc aaggccagag 2940 ttaagcagat gttacgccct gctgcaggcc tttctattat ccaatagttg gttgatcttt 3000 ggaaagagcc tcggctgagg cccatactga aagagatcga tggatatcga tatgccatgt 3060 tgtttgctag ccagaaccag atcacatccg atatgctatt gcagcttgac gcagatatgg 3120 aggataagtt gattcatggg atcgctcagg agtagctcat ccatgcacgc cgacaagaac 3180 agaaattccg tcgagttaac gcagccgctt acgacggatt cgaaggtcat ccattcggaa 3240 tgtattagtt tgcaccagct ccgcgtcaca cctgtcttca tttgaataag atgttagcaa 3300 ttgtttttag ctttgtcttg ttgtggcagg gcggcaagtg cttcagacat cattctgttt 3360 tcaaatttta tgctggagaa cagcttctta attcctttgg aaataataga ctgcgtctta 3420 aaattcagat gtctggatat agatatgatt gtaaaataac ctatttaagt gtcatttaga 3480 acataagttt tatgaatgtt cttccatttt cgtcatcgaa cgaataagag taaatacacc 3540 ttttttaaca ttacaaataa gttcttatac gttgtttata caccgggaat catttccatt 3600 attttcgcgc aaaagtcacg gatattcgtg aaagcgacat aaactgcgaa atttgcgggg 3660 agtgtcttga gtttgcctcg aggctagcgc atgcacatag acacacacat catctcattg 3720 atgcttggta ataattgtca ttagattgtt tttatgcata gatgcactcg aaatcagcca 3780 attttagaca agtatcaaac ggatgtgact tcagtacatt aaaaacgtcc gcaatgtgtt 3840 attaagttgt ctaagcgtca atttgattta caattgaata tatcctgccc cagccagcca 3900 acagctcgat ttacaattga atatatcctg ccggccggcc cacgcgtgtc gaggaattct 3960 gatctggccc ccatttggac gtgaatgtag acacgtcgaa ataaagattt ccgaattaga 4020 ataatttgtt tattgctttc gcctataaat acgacggatc gtaatttgtc gttttatcaa 4080 aatgtacttt cattttataa taacgctgcg gacatctaca tttttgaatt gaaaaaaaat 4140 tggtaattac tctttctttt tctccatatt gaccatcata ctcattgctg atccatgtag 4200 atttcccgga catgaagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4260 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4320 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4380 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4440 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 4500 caggtacaat cgagccgacg ttcacgcgga acgaccaagc aagcttggct gccatttttg 4560 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 4620 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 4680 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 4740 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 4800 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 4860 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 4920 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 4980 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 5040 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 5100 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 5160 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 5220 gcggcgaggg caaccagccc ggtgagcgtc ggaaagggtc gacggatctt ttccgctgca 5280 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 5340 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 5400 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 5460 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc 5520 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc 5580 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg 5640 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg 5700 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc 5760 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat 5820 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc 5880 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg 5940 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc 6000 ggagctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 6060 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 6120 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 6180 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 6240 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 6300 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 6360 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 6420 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 6480 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 6540 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 6600 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 6660 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 6720 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 6780 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 6840 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 6900 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 6960 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 7020 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 7080 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 7140 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 7200 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 7260 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 7320 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 7380 tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 7440 ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 7500 gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc 7560 cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag 7620 aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac 7680 atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct 7740 cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc 7800 ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt 7860 ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc 7920 tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc 7980 gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga 8040 ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca 8100 ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg 8160 gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc 8220 gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg 8280 ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga 8340 gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt 8400 tccttactgg gctttctcag cccccgagcg cttagtggga atttgtaccc cttatcgaac 8460 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct 8520 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 8580 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 8640 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 8700 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 8760 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 8820 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 8880 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 8940 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 9000 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 9060 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 9120 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 9180 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac 9240 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag 9300 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 9360 tcgcggcgcg gcttaactca agcgttagag agctggggaa gactatgcgc gatctgttga 9420 aggtggttct aagcctcgtc ttgcgatggc atttcgatcc attcccattc cgcgctcaag 9480 atggcttccc ctcggcagtt catcagggct aaatcaatct agccgacttg tccggtgaaa 9540 tgggctgcac tccaacagaa acaatcaaac aaacatacac agcgacttat tcacacgagc 9600 tcaaattaca acggtatata tcctgccagt cagcatcatc acaccaaaag ttaggcccga 9660 atagtttgaa attagaaagc tcgcaattga ggtctacagg ccaaattcgc tcttagccgt 9720 acaatattac tcaccggatc ctaaccggtt taattaaggc gcgccatgcc cgggcaagcg 9780 gccgcacaag tttgtacaaa aaagcaggct tctagaccta gctaggtgga gtcatcacgc 9840 agactatctc agcatgtgcg tagcacgcgg ccgcagctta tagtaaagat ttttttgtta 9900 ataaaatgaa agttaatatt tatggattta tttgaacata tttacaagtt tttgttaata 9960 aatttattta cagaagtgtt aaatttgaaa atgtgttaaa tgagagttaa tatttatgga 10020 tataatatta ctttatttaa aaataaaatg aatttatatt aaaatatgat ttggtaaaag 10080 atattataaa gaggaacttg gatgagaaag acgacgatgg gcaaaagttg catggcacac 10140 cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc acactcacca tgcatgctgc 10200 cacgtcagct ttcacactat gacaccactg gccatacacg ttgccacctc agctttctcc 10260 tcttctcact atgacacgac tggccatgca tgcatgcatg ctgccacctc agctcccgcc 10320 tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg ccaacctctt ctctctataa 10380 atagagagaa gagaatgatg gttaaactca aaccaaaaca agaaaacata cacaaatagc 10440 aaaacggatc cacaaccaaa aatggcggcc cgtctgcagg agcaaaaggg aggcgagatg 10500 gatacccgca ttgccatcat cggcatgtcg gccatcctcc cctgcggcac gaccgtgcgc 10560 gagtcgtggg agaccatccg cgccggcatc gactgcctgt cggatctccc cgaggaccgc 10620 gtcgacgtga cggcgtactt tgaccccgtc aagaccacca aggacaagat ctactgcaag 10680 cgcggtggct tcattcccga gtacgacttt gacgcccgcg agttcggact caacatgttc 10740 cagatggagg actcggacgc aaaccagacc atctcgcttc tcaaggtcaa ggaggccctc 10800 caggacgccg gcatcgacgc cctcggcaag gaaaagaaga acatcggctg cgtgctcggc 10860 attggcggcg gccaaaagtc cagccacgag ttctactcgc gccttaatta tgttgtcgtg 10920 gagaaggtcc tccgcaagat gggcatgccc gaggaggacg tcaaggtcgc cgtcgaaaag 10980 tacaaggcca acttccccga gtggcgcctc gactccttcc ctggcttcct cggcaacgtc 11040 accgccggtc gctgcaccaa caccttcaac ctcgacggca tgaactgcgt tgtcgacgcc 11100 gcatgcgcct cgtccctcat cgccgtcaag gtcgccatcg acgagctgct ctacggtgac 11160 tgcgacatga tggtcaccgg tgccacctgc acggataact ccatcggcat gtacatggcc 11220 ttctccaaga cccccgtgtt ctccacggac cccagcgtgc gcgcctacga cgaaaagaca 11280 aagggcatgc tcatcggcga gggctccgcc atgctcgtcc tcaagcgcta cgccgacgcc 11340 gtccgcgacg gcgatgagat ccacgctgtt attcgcggct gcgcctcctc cagtgatggc 11400 aaggccgccg gcatctacac gcccaccatt tcgggccagg aggaggccct ccgccgcgcc 11460 tacaaccgcg cctgtgtcga cccggccacc gtcactctcg tcgagggtca cggcaccggt 11520 actcccgttg gcgaccgcat cgagctcacc gccttgcgca acctctttga caaggcctac 11580 ggcgagggca acaccgaaaa ggtcgctgtg ggcagcatca agtccagcat cggccatctc 11640 aaggccgtcg ccggtctcgc cggtatgatc aaggtcatca tggcgctcaa gcacaagact 11700 ctcccgggca ccatcaacgt cgacaaccca cccaacctct acgacaacac gcccatcaac 11760 gagtcctcgc tctacattaa caccatgaac cgcccctggt tcccgccccc tggtgtgccc 11820 cgccgcgccg gcatttcgag ctttggcttt ggtggcgcca actaccacgc cgtcctcgag 11880 gaggccgagc ccgagcacac gaccgcgtac cgcctcaaca agcgcccgca gcccgtgctc 11940 atgatggccg ccacgcccgc ggccctccag tcgctctgcg aggcccagct caaggagttc 12000 gaggccgcca tcaaggagaa cgagaccgtc aagaacaccg cctacatcaa gtgcgtcaag 12060 ttcggcgagc agttcaaatt ccctggctcc atcccggcca caaacgcgcg cctcggcttc 12120 ctcgtcaagg atgctgagga tgcctgctcc accctccgtg ccatctgcgc ccaattcgcc 12180 aaggatgtca ccaaggaggc ctggcgcctc ccccgcgagg gcgtcagctt ccgcgccaag 12240 ggcatcgcca ccaacggcgc tgtcgccgcg ctcttctccg gccagggcgc gcagtacacg 12300 cacatgttta gcgaggtggc catgaactgg ccccagttcc gccagagcat tgccgccatg 12360 gacgccgccc agtccaaggt cgctggaagc gacaaggact ttgagcgcgt ctcccaggtc 12420 ctctacccgc gcaagccgta cgagcgtgag cccgagcagg accacaagaa gatctccctc 12480 accgcctact cgcagccctc gaccctggcc tgcgctctcg gtgcctttga gatcttcaag 12540 gaggccggct tcaccccgga ctttgccgcc ggccattcgc tcggtgagtt cgccgccctc 12600 tacgccgcgg gctgcgtcga ccgcgacgag ctctttgagc ttgtctgccg ccgcgcccgc 12660 atcatgggcg gcaaggacgc accggccacc cccaagggct gcatggccgc cgtcattggc 12720 cccaacgccg agaacatcaa ggtccaggcc gccaacgtct ggctcggcaa ctccaactcg 12780 ccttcgcaga ccgtcatcac cggctccgtc gaaggtatcc aggccgagag cgcccgcctc 12840 cagaaggagg gcttccgcgt cgtgcctctt gcctgcgaga gcgccttcca ctcgccccag 12900 atggagaacg cctcgtcggc cttcaaggac gtcatctcca aggtctcctt ccgcaccccc 12960 aaggccgaga ccaagctctt cagcaacgtc tctggcgaga cctaccccac ggacgcccgc 13020 gagatgctta cgcagcacat gaccagcagc gtcaagttcc tcacccaggt ccgcaacatg 13080 caccaggccg gtgcgcgcat ctttgtcgag ttcggaccca agcaggtgct ctccaagctt 13140 gtctccgaga ccctcaagga tgacccctcg gttgtcaccg tctctgtcaa cccggcctcg 13200 ggcacggatt cggacatcca gctccgcgac gcggccgtcc agctcgttgt cgctggcgtc 13260 aaccttcagg gctttgacaa gtgggacgcc cccgatgcca cccgcatgca ggccatcaag 13320 aagaagcgca ctaccctccg cctttcggcc gccacctacg tctcggacaa gaccaagaag 13380 gtccgcgacg ccgccatgaa cgatggccgc tgcgtcacct acctcaaggg cgccgcaccg 13440 ctcatcaagg ccccggagcc cgttgtcgac gaggccgcca agcgcgaggc cgagcgtctc 13500 cagaaggagc ttcaggatgc ccagcgccag ctcgacgacg ccaagcgcgc cgccgccgag 13560 gccaactcca agctcgccgc tgccaaggag gaggccaaga ccgccgctgc ttcggccaag 13620 cccgcagttg acactgctgt tgtcgaaaag catcgtgcca tcctcaagtc catgctcgcg 13680 gagctcgatg gctacggatc ggtcgacgct tcttccctcc agcagcagca gcagcagcag 13740 acggcccccg ccccggtcaa ggctgctgcg cctgccgccc ccgttgcctc ggcccctgcc 13800 ccggctgtct cgaacgagct tcttgagaag gccgagactg tcgtcatgga ggtcctcgcc 13860 gccaagaccg gctacgagac cgacatgatc gaggctgaca tggagctcga gaccgagctc 13920 ggcattgact ccatcaagcg tgtcgagatc ctctccgagg tccaggccat gctcaatgtc 13980 gaggccaagg atgtcgatgc cctcagccgc actcgcactg ttggtgaggt tgtcaacgcc 14040 atgaaggccg agatcgctgg cagctctgcc ccggcgcctg ctgccgctgc tccggctccg 14100 gccaaggctg cccctgccgc cgctgcgcct gctgtctcga acgagcttct cgagaaggcc 14160 gagaccgtcg tcatggaggt cctcgccgcc aagactggct acgagactga catgatcgag 14220 tccgacatgg agctcgagac tgagctcggc attgactcca tcaagcgtgt cgagatcctc 14280 tccgaggttc aggccatgct caacgtcgag gccaaggacg tcgacgctct cagccgcact 14340 cgcactgtgg gtgaggtcgt caacgccatg aaggctgaga tcgctggtgg ctctgccccg 14400 gcgcctgccg ccgctgcccc aggtccggct gctgccgccc ctgcgcctgc cgccgccgcc 14460 cctgctgtct cgaacgagct tcttgagaag gccgagaccg tcgtcatgga ggtcctcgcc 14520 gccaagactg gctacgagac tgacatgatc gagtccgaca tggagctcga gaccgagctc 14580 ggcattgact ccatcaagcg tgtcgagatt ctctccgagg tccaggccat gctcaacgtc 14640 gaggccaagg acgtcgacgc tctcagccgc acccgcactg ttggcgaggt cgtcgatgcc 14700 atgaaggccg agatcgctgg tggctctgcc ccggcgcctg ccgccgctgc tcctgctccg 14760 gctgctgccg cccctgcgcc tgccgcccct gcgcctgctg tctcgagcga gcttctcgag 14820 aaggccgaga ctgtcgtcat ggaggtcctc gccgccaaga ctggctacga gactgacatg 14880 atcgagtccg acatggagct cgagaccgag ctcggcattg actccatcaa gcgtgtcgag 14940 attctctccg aggtccaggc catgctcaac gtcgaggcca aggacgtcga cgctctcagc 15000 cgcacccgca ctgttggcga ggtcgtcgat gccatgaagg ccgagatcgc tggtggctct 15060 gccccggcgc ctgccgccgc tgctcctgct ccggctgctg ccgcccctgc gcctgccgcc 15120 cctgcgcctg ccgcccctgc gcctgctgtc tcgagcgagc ttctcgagaa ggccgagact 15180 gtcgtcatgg aggtcctcgc cgccaagact ggctacgaga ctgacatgat tgagtccgac 15240 atggagctcg agaccgagct cggcattgac tccatcaagc gtgtcgagat tctctccgag 15300 gttcaggcca tgctcaacgt cgaggccaag gacgtcgacg ctctcagccg cactcgcact 15360 gttggtgagg tcgtcgatgc catgaaggct gagatcgctg gcagctccgc ctcggcgcct 15420 gccgccgctg ctcctgctcc ggctgctgcc gctcctgcgc ccgctgccgc cgcccctgct 15480 gtctcgaacg agcttctcga gaaagccgag actgtcgtca tggaggtcct cgccgccaag 15540 actggctacg agactgacat gatcgagtcc gacatggagc tcgagactga gctcggcatt 15600 gactccatca agcgtgtcga gatcctctcc gaggttcagg ccatgctcaa cgtcgaggcc 15660 aaggacgtcg atgccctcag ccgcacccgc actgttggcg aggttgtcga tgccatgaag 15720 gccgagatcg ctggtggctc tgccccggcg cctgccgccg ctgcccctgc tccggctgcc 15780 gccgcccctg ctgtctcgaa cgagcttctc gagaaggccg agactgtcgt catggaggtc 15840 ctcgccgcca agactggcta cgagaccgac atgatcgagt ccgacatgga gctcgagacc 15900 gagctcggca ttgactccat caagcgtgtc gagattctct ccgaggttca ggccatgctc 15960 aacgtcgagg ccaaggacgt cgatgctctc agccgcactc gcactgttgg cgaggtcgtc 16020 gatgccatga aggctgagat cgccggcagc tccgccccgg cgcctgccgc cgctgctcct 16080 gctccggctg ctgccgctcc tgcgcccgct gccgctgccc ctgctgtctc gagcgagctt 16140 ctcgagaagg ccgagaccgt cgtcatggag gtcctcgccg ccaagactgg ctacgagact 16200 gacatgattg agtccgacat ggagctcgag actgagctcg gcattgactc catcaagcgt 16260 gtcgagatcc tctccgaggt tcaggccatg ctcaacgtcg aggccaagga cgtcgatgcc 16320 ctcagccgca cccgcactgt tggcgaggtt gtcgatgcca tgaaggccga gatcgctggt 16380 ggctctgccc cggcgcctgc cgccgctgcc cctgctccgg ctgccgccgc ccctgctgtc 16440 tcgaacgagc ttcttgagaa ggccgagacc gtcgtcatgg aggtcctcgc cgccaagact 16500 ggctacgaga ccgacatgat cgagtccgac atggagctcg agaccgagct cggcattgac 16560 tccatcaagc gtgtcgagat tctctccgag gttcaggcca tgctcaacgt cgaggccaag 16620 gacgtcgacg ctctcagccg cactcgcact gttggcgagg tcgtcgatgc catgaaggct 16680 gagatcgctg gtggctctgc cccggcgcct gccgccgctg ctcctgcctc ggctggcgcc 16740 gcgcctgcgg tcaagattga ctcggtccac ggcgctgact gtgatgatct ttccctgatg 16800 cacgccaagg tggttgacat ccgccgcccg gacgagctca tcctggagcg ccccgagaac 16860 cgccccgttc tcgttgtcga tgacggcagc gagctcaccc tcgccctggt ccgcgtcctc 16920 ggcgcctgcg ccgttgtcct gacctttgag ggtctccagc tcgctcagcg cgctggtgcc 16980 gctgccatcc gccacgtgct cgccaaggat ctttccgcgg agagcgccga gaaggccatc 17040 aaggaggccg agcagcgctt tggcgctctc ggcggcttca tctcgcagca ggcggagcgc 17100 ttcgagcccg ccgaaatcct cggcttcacg ctcatgtgcg ccaagttcgc caaggcttcc 17160 ctctgcacgg ctgtggctgg cggccgcccg gcctttatcg gtgtggcgcg ccttgacggc 17220 cgcctcggat tcacttcgca gggcacttct gacgcgctca agcgtgccca gcgtggtgcc 17280 atctttggcc tctgcaagac catcggcctc gagtggtccg agtctgacgt cttttcccgc 17340 ggcgtggaca ttgctcaggg catgcacccc gaggatgccg ccgtggcgat tgtgcgcgag 17400 atggcgtgcg ctgacattcg cattcgcgag gtcggcattg gcgcaaacca gcagcgctgc 17460 acgatccgtg ccgccaagct cgagaccggc aacccgcagc gccagatcgc caaggacgac 17520 gtgctgctcg tttctggcgg cgctcgcggc atcacgcctc tttgcatccg ggagatcacg 17580 cgccagatcg cgggcggcaa gtacattctg cttggccgca gcaaggtctc tgcgagcgaa 17640 ccggcatggt gcgctggcat cactgacgag aaggctgtgc aaaaggctgc tacccaggag 17700 ctcaagcgcg cctttagcgc tggcgagggc cccaagccca cgccccgcgc tgtcactaag 17760 cttgtgggct ctgttcttgg cgctcgcgag gtgcgcagct ctattgctgc gattgaagcg 17820 ctcggcggca aggccatcta ctcgtcgtgc gacgtgaact ctgccgccga cgtggccaag 17880 gccgtgcgcg atgccgagtc ccagctcggt gcccgcgtct cgggcatcgt tcatgcctcg 17940 ggcgtgctcc gcgaccgtct catcgagaag aagctccccg acgagttcga cgccgtcttt 18000 ggcaccaagg tcaccggtct cgagaacctc ctcgccgccg tcgaccgcgc caacctcaag 18060 cacatggtcc tcttcagctc gctcgccggc ttccacggca acgtcggcca gtctgactac 18120 gccatggcca acgaggccct taacaagatg ggcctcgagc tcgccaagga cgtctcggtc 18180 aagtcgatct gcttcggtcc ctgggacggt ggcatggtga cgccgcagct caagaagcag 18240 ttccaggaga tgggcgtgca gatcatcccc cgcgagggcg gcgctgatac cgtggcgcgc 18300 atcgtgctcg gctcctcgcc ggctgagatc cttgtcggca actggcgcac cccgtccaag 18360 aaggtcggct cggacaccat caccctgcac cgcaagattt ccgccaagtc caaccccttc 18420 ctcgaggacc acgtcatcca gggccgccgc gtgctgccca tgacgctggc cattggctcg 18480 ctcgcggaga cctgcctcgg cctcttcccc ggctactcgc tctgggccat tgacgacgcc 18540 cagctcttca agggtgtcac tgtcgacggc gacgtcaact gcgaggtgac cctcaccccg 18600 tcgacggcgc cctcgggccg cgtcaacgtc caggccacgc tcaagacctt ttccagcggc 18660 aagctggtcc cggcctaccg cgccgtcatc gtgctctcca accagggcgc gcccccggcc 18720 aacgccacca tgcagccgcc ctcgctcgat gccgatccgg cgctccaggg ctccgtctac 18780 gacggcaaga ccctcttcca cggcccggcc ttccgcggca tcgatgacgt gctctcgtgc 18840 accaagagcc agcttgtggc caagtgcagc gctgtccccg gctccgacgc cgctcgcggc 18900 gagtttgcca cggacactga cgcccatgac cccttcgtga acgacctggc ctttcaggcc 18960 atgctcgtct gggtgcgccg cacgctcggc caggctgcgc tccccaactc gatccagcgc 19020 atcgtccagc accgcccggt cccgcaggac aagcccttct acattaccct ccgctccaac 19080 cagtcgggcg gtcactccca gcacaagcac gcccttcagt tccacaacga gcagggcgat 19140 ctcttcattg atgtccaggc ttcggtcatc gccacggaca gccttgcctt ctaaatctaa 19200 actaggccat ggtgacttaa ggtagttagc ttaatcactt aggtcaccca gtccaagccc 19260 ggcctagcac gatgatcatg agtgttgcta ttgatgtatg ttaatactat acacagtcat 19320 cgtgtgtgtt ttataaaact gtaagcttag gatgtttgag gctaatgtaa ttagcactac 19380 accgtaataa atgagaggtt tactttatgt tttaataatg ttcttaccct tgctgctctc 19440 taaactgtaa tggcgtacgg ttagctaaac taaagtaaaa atcgattcgc ttgagtaaaa 19500 ttagtgaccg ggaaagatca tcgactacta ctgctcagag atgtaatcgg taattaagac 19560 ttcaagctca ataattaatt aggagtaaat ctggcttcga atttttaaat tgtcaacttc 19620 caaagaagga ttggcaagac tcaatctcaa ttgcccaaca aacatatggc tctttacaaa 19680 tttggaatct ctttttgatg gcaagaacgt cgtaattcct ctgtcgatct catgtaatta 19740 ccaaactcat ctacttatta ctttctttat atgcacttac tgatgcggcc gcttaattaa 19800 gatgagtgat actcaggact caggactcac tctgctgatc actagtgcta gcctcgaggt 19860 cgacacaact ttgtatacaa aagttgtcta gacctagcta ggctcagcaa ctcatccagt 19920 caagttcgag tcagtgagtt gggcggccgc agcttatagt aaagattttt ttgttaataa 19980 aatgaaagtt aatatttatg gatttatttg aacatattta caagtttttg ttaataaatt 20040 tatttacaga agtgttaaat ttgaaaatgt gttaaatgag agttaatatt tatggatata 20100 atattacttt atttaaaaat aaaatgaatt tatattaaaa tatgatttgg taaaagatat 20160 tataaagagg aacttggatg agaaagacga cgatgggcaa aagttgcatg gcacaccgct 20220 tttgttcagt aacatctgca ctgtggcgcc tttttcacac tcaccatgca tgctgccacg 20280 tcagctttca cactatgaca ccactggcca tacacgttgc cacctcagct ttctcctctt 20340 ctcactatga cacgactggc catgcatgca tgcatgctgc cacctcagct cccgcctctt 20400 cacccgtgtc tttctctcat gtcgcagtgc tgcgtgccaa cctcttctct ctataaatag 20460 agagaagaga atgatggtta aactcaaacc aaaacaagaa aacatacaca aatagcaaaa 20520 cggatccaca accaaaaatg gccgctcgca acgtgtctgc agcgcatgag atgcacgatg 20580 aaaagcgcat cgccgtcgtc ggcatggccg tccagtacgc cggatgcaaa accaaggacg 20640 agttctggga ggtgctcatg aacggcaagg tcgagtccaa ggtgatcagc gacaaacgac 20700 tcggctccaa ctaccgcgcc gagcactaca aagcagagcg cagcaagtat gccgacacct 20760 tttgcaacga aacgtacggc acccttgacg agaacgagat cgacaacgag cacgaactcc 20820 tcctcaacct cgccaagcag gcactcgcag agacatccgt caaagactcg acacgctgcg 20880 gcatcgtcag cggctgcctc tcgttcccca tggacaacct ccagggtgaa ctcctcaacg 20940 tgtaccaaaa ccatgtcgag aaaaagctcg gggcccgcgt cttcaaggac gcctcccatt 21000 ggtccgaacg cgagcagtcc aacaaacccg aggccggtga ccgccgcatc ttcatggacc 21060 cggcctcctt cgtcgccgaa gaactcaacc tcggcgccct tcactactcc gtcgacgcag 21120 catgcgccac ggcgctctac gtgctccgcc tcgcgcagga tcatctcgtc tccggcgccg 21180 ccgacgtcat gctctgcggt gccacctgcc tgccggagcc ctttttcatc ctttcgggct 21240 tttccacctt ccaggccatg cccgtcggca cgggccagaa cgtgtccatg ccgctgcaca 21300 aggacagcca gggcctcacc ccgggtgagg gcggctccat catggtcctc aagcgtctcg 21360 atgatgccat ccgcgacggc gaccacatct acggcaccct tctcggcgcc aatgtcagca 21420 actccggcac aggtctgccc ctcaagcccc ttctccccag cgagaaaaag tgcctcatgg 21480 acacctacac gcgcattaac gtgcacccgc acaagattca gtacgtcgag tgccacgcca 21540 ccggcacgcc ccagggtgat cgtgtggaaa tcgacgccgt caaggcctgc tttgaaggca 21600 aggtcccccg tttcggtacc acaaagggca actttggaca caccctcgtc gcagccggct 21660 ttgccggtat gtgcaaggtc ctcctctcca tgaagcatgg catcatcccg cccaccccgg 21720 gtatcgatga cgagaccaag atggaccctc tcgtcgtctc cggtgaggcc atcccatggc 21780 cagagaccaa cggcgagccc aagcgcgccg gtctctcggc ctttggcttt ggtggcacca 21840 acgcccatgc cgtctttgag gagcatgacc cctccaacgc cgcctgcacg ggccacgact 21900 ccatttctgc gctctcggcc cgctgcggcg gtgaaagcaa catgcgcatc gccatcactg 21960 gtatggacgc cacctttggc gctctcaagg gactcgacgc cttcgagcgc gccatttaca 22020 ccggcgctca cggtgccatc ccactcccag aaaagcgctg gcgctttctc ggcaaggaca 22080 aggactttct tgacctctgc ggcgtcaagg ccaccccgca cggctgctac attgaagatg 22140 ttgaggtcga cttccagcgc ctccgcacgc ccatgacccc tgaagacatg ctcctccctc 22200 agcagcttct ggccgtcacc accattgacc gcgccatcct cgactcggga atgaaaaagg 22260 gtggcaatgt cgccgtcttt gtcggcctcg gcaccgacct cgagctctac cgtcaccgtg 22320 ctcgcgtcgc tctcaaggag cgcgtccgcc ctgaagcctc caagaagctc aatgacatga 22380 tgcagtacat taacgactgc ggcacatcca catcgtacac ctcgtacatt ggcaacctcg 22440 tcgccacgcg cgtctcgtcg cagtggggct tcacgggccc ctcctttacg atcaccgagg 22500 gcaacaactc cgtctaccgc tgcgccgagc tcggcaagta cctcctcgag accggcgagg 22560 tcgatggcgt cgtcgttgcg ggtgtcgatc tctgcggcag tgccgaaaac ctttacgtca 22620 agtctcgccg cttcaaggtg tccacctccg ataccccgcg cgccagcttt gacgccgccg 22680 ccgatggcta ctttgtcggc gagggctgcg gtgcctttgt gctcaagcgt gagactagct 22740 gcaccaagga cgaccgtatc tacgcttgca tggatgccat cgtccctggc aacgtcccta 22800 gcgcctgctt gcgcgaggcc ctcgaccagg cgcgcgtcaa gccgggcgat atcgagatgc 22860 tcgagctcag cgccgactcc gcccgccacc tcaaggaccc gtccgtcctg cccaaggagc 22920 tcactgccga ggaggaaatc ggcggccttc agacgatcct tcgtgacgat gacaagctcc 22980 cgcgcaacgt cgcaacgggc agtgtcaagg ccaccgtcgg tgacaccggt tatgcctctg 23040 gtgctgccag cctcatcaag gctgcgcttt gcatctacaa ccgctacctg cccagcaacg 23100 gcgacgactg ggatgaaccc gcccctgagg cgccctggga cagcaccctc tttgcgtgcc 23160 agacctcgcg cgcttggctc aagaaccctg gcgagcgtcg ctatgcggcc gtctcgggcg 23220 tctccgagac gcgctcgtgc tattccgtgc tcctctccga agccgagggc cactacgagc 23280 gcgagaaccg catctcgctc gacgaggagg cgcccaagct cattgtgctt cgcgccgact 23340 cccacgagga gatccttggt cgcctcgaca agatccgcga gcgcttcttg cagcccacgg 23400 gcgccgcccc gcgcgagtcc gagctcaagg cgcaggcccg ccgcatcttc ctcgagctcc 23460 tcggcgagac ccttgcccag gatgccgctt cttcaggctc gcaaaagccc ctcgctctca 23520 gcctcgtctc cacgccctcc aagctccagc gcgaggtcga gctcgcggcc aagggtatcc 23580 cgcgctgcct caagatgcgc cgcgattgga gctcccctgc tggcagccgc tacgcgcctg 23640 agccgctcgc cagcgaccgc gtcgccttca tgtacggcga aggtcgcagc ccttactacg 23700 gcatcaccca agacattcac cgcatttggc ccgaactcca cgaggtcatc aacgaaaaga 23760 cgaaccgtct ctgggccgaa ggcgaccgct gggtcatgcc gcgcgccagc ttcaagtcgg 23820 agctcgagag ccagcagcaa gagtttgatc gcaacatgat tgaaatgttc cgtcttggaa 23880 tcctcacctc aattgccttc accaatctgg cgcgcgacgt tctcaacatc acgcccaagg 23940 ccgcctttgg cctcagtctt ggcgagattt ccatgatttt tgccttttcc aagaagaacg 24000 gtctcatctc cgaccagctc accaaggatc ttcgcgagtc cgacgtgtgg aacaaggctc 24060 tggccgttga atttaatgcg ctgcgcgagg cctggggcat tccacagagt gtccccaagg 24120 acgagttctg gcaaggctac attgtgcgcg gcaccaagca ggatatcgag gcggccatcg 24180 ccccggacag caagtacgtg cgcctcacca tcatcaatga tgccaacacc gccctcatta 24240 gcggcaagcc cgacgcctgc aaggctgcga tcgcgcgtct cggtggcaac attcctgcgc 24300 ttcccgtgac ccagggcatg tgcggccact gccccgaggt gggaccttat accaaggata 24360 tcgccaagat ccatgccaac cttgagttcc ccgttgtcga cggccttgac ctctggacca 24420 caatcaacca gaagcgcctc gtgccacgcg ccacgggcgc caaggacgaa tgggcccctt 24480 cttcctttgg cgagtacgcc ggccagctct acgagaagca ggctaacttc ccccaaatcg 24540 tcgagaccat ttacaagcaa aactacgacg tctttgtcga ggttgggccc aacaaccacc 24600 gtagcaccgc agtgcgcacc acgcttggtc cccagcgcaa ccaccttgct ggcgccatcg 24660 acaagcagaa cgaggatgct tggacgacca tcgtcaagct tgtggcttcg ctcaaggccc 24720 accttgttcc tggcgtcacg atctcgccgc tgtaccactc caagcttgtg gcggaggctg 24780 aggcttgcta cgctgcgctc tgcaagggtg aaaagcccaa gaagaacaag tttgtgcgca 24840 agattcagct caacggtcgc ttcaacagca aggcggaccc catctcctcg gccgatcttg 24900 ccagctttcc gcctgcggac cctgccattg aagccgccat ctcgagccgc atcatgaagc 24960 cggttgctcc gaagttctac gcgcgtctca acattgacga gcaggacgag acccgtgatc 25020 cgatcctcaa caaggacaac gcgccgtctt ccagctctag ctcctcttcc agctcttcca 25080 gctcttccag cccgtcgcca gctccgtccg ccccagtgca aaagaaggct gctccggccg 25140 cggagaccaa ggctgttgct tcggctgacg cacttcgcag tgccctgctc gatctcgaca 25200 gtatgcttgc gctgagctct gccagtgcct ccggcaacct tgttgagact gcgcctagcg 25260 acgcctcggt cattgtgccg ccctgcaaca ttgcggatct cggcagccgc gccttcatga 25320 aaacgtacgg tgtttcggcg cctctgtaca cgggcgccat ggccaagggc attgcctctg 25380 cggacctcgt cattgccgcc ggccgccagg gcatccttgc gtcctttggc gccggcggac 25440 ttcccatgca ggttgtgcgt gagtccatcg aaaagattca ggccgccctg cccaatggcc 25500 cgtacgctgt caaccttatc cattctccct ttgacagcaa cctcgaaaag ggcaatgtcg 25560 atctcttcct cgagaagggt gtcacctttg tcgaggcctc ggcctttatg acgctcaccc 25620 cgcaggtcgt gcggtaccgc gcggctggcc tcacgcgcaa cgccgacggc tcggtcaaca 25680 tccgcaaccg tatcattggc aaggtctcgc gcaccgagct cgccgagatg ttcatgcgtc 25740 ctgcgcccga gcaccttctt cagaagctca ttgcttccgg cgagatcaac caggagcagg 25800 ccgagctcgc ccgccgtgtt cccgtcgctg acgacatcgc ggtcgaagct gactcgggtg 25860 gccacaccga caaccgcccc atccacgtca ttctgcccct catcatcaac cttcgcgacc 25920 gccttcaccg cgagtgcggc tacccggcca accttcgcgt ccgtgtgggc gccggcggtg 25980 gcattgggtg cccccaggcg gcgctggcca ccttcaacat gggtgcctcc tttattgtca 26040 ccggcaccgt gaaccaggtc gccaagcagt cgggcacgtg cgacaatgtg cgcaagcagc 26100 tcgcgaaggc cacttactcg gacgtatgca tggccccggc tgccgacatg ttcgaggaag 26160 gcgtcaagct tcaggtcctc aagaagggaa ccatgtttcc ctcgcgcgcc aacaagctct 26220 acgagctctt ttgcaagtac gactcgttcg agtccatgcc ccccgcagag cttgcgcgcg 26280 tcgagaagcg catcttcagc cgcgcgctcg aagaggtctg ggacgagacc aaaaactttt 26340 acattaaccg tcttcacaac ccggagaaga tccagcgcgc cgagcgcgac cccaagctca 26400 agatgtcgct gtgctttcgc tggtacctga gcctggcgag ccgctgggcc aacactggag 26460 cttccgatcg cgtcatggac taccaggtct ggtgcggtcc tgccattggt tccttcaacg 26520 atttcatcaa gggaacttac cttgatccgg ccgtcgcaaa cgagtacccg tgcgtcgttc 26580 agattaacaa gcagatcctt cgtggagcgt gcttcttgcg ccgtctcgaa attctgcgca 26640 acgcacgcct ttccgatggc gctgccgctc ttgtggccag catcgatgac acatacgtcc 26700 cggccgagaa gctgtaagcg gccgcattta aatggatcca ctaggccatg gtgacttaag 26760 gtagttagct taatcactta ggtcacccag tccaagcccg gcctagcacg atgatcatga 26820 gtgttgctat tgatgtatgt taatactata cacagtcatc gtgtgtgttt tataaaactg 26880 taagcttagg atgtttgagg ctaatgtaat tagcactaca ccgtaataaa tgagaggttt 26940 actttatgtt ttaataatgt tcttaccctt gctgctctct aaactgtaat ggcgtacggt 27000 tagctaaact aaagtaaaaa tcgattcgct tgagtaaaat tagtgaccgg gaaagatcat 27060 cgactactac tgctcagaga tgtaatcggt aattaagact tcaagctcaa taattaatta 27120 ggagtaaatc tggcttcgaa tttttaaatt gtcaacttcc aaagaaggat tggcaagact 27180 caatctcaat tgcccaacaa acatatggct ctttacaaat ttggaatctc tttttgatgg 27240 caagaacgtc gtaattcctc tgtcgatctc atgtaattac caaactcatc tacttattac 27300 tttctttata tgcacttact gatgcggccg cttaattaat ctgacctgac cactagacct 27360 gagtgagtac agatgtgcta ctagtgctag cctcgaggtc gaccaacttt tctatacaaa 27420 gttgtctaga cctagctagg actaggcact gagtgactca ctgacctgac tgagtgactg 27480 gcggccgcag cttatagtaa agattttttt gttaataaaa tgaaagttaa tatttatgga 27540 tttatttgaa catatttaca agtttttgtt aataaattta tttacagaag tgttaaattt 27600 gaaaatgtgt taaatgagag ttaatattta tggatataat attactttat ttaaaaataa 27660 aatgaattta tattaaaata tgatttggta aaagatatta taaagaggaa cttggatgag 27720 aaagacgacg atgggcaaaa gttgcatggc acaccgcttt tgttcagtaa catctgcact 27780 gtggcgcctt tttcacactc accatgcatg ctgccacgtc agctttcaca ctatgacacc 27840 actggccata cacgttgcca cctcagcttt ctcctcttct cactatgaca cgactggcca 27900 tgcatgcatg catgctgcca cctcagctcc cgcctcttca cccgtgtctt tctctcatgt 27960 cgcagtgctg cgtgccaacc tcttctctct ataaatagag agaagagaat gatggttaaa 28020 ctcaaaccaa aacaagaaaa catacacaaa tagcaaaacg gatccacaac caaaacatat 28080 ggcgctccgt gtcaagacga acaagaagcc atgctgggag atgaccaagg aggagctgac 28140 cagcggcaag accgaggtgt tcaactatga ggaactcctc gagttcgcag agggcgacat 28200 cgccaaggtc ttcggacccg agttcgccgt catcgacaag tacccgcgcc gcgtgcgcct 28260 gcccgcccgc gagtacctgc tcgtgacccg cgtcaccctc atggacgccg aggtcaacaa 28320 ctaccgcgtc ggcgcccgca tggtcaccga gtacgatctc cccgtcaacg gagagctctc 28380 cgagggcgga gactgcccct gggccgtcct ggtcgagagt ggccagtgcg atctcatgct 28440 catctcctac atgggcattg acttccagaa ccagggcgac cgcgtctacc gcctgctcaa 28500 caccacgctc accttttacg gcgtggccca cgagggcgag accctcgagt acgacattcg 28560 cgtcaccggc ttcgccaagc gtctcgacgg cggcatctcc atgttcttct tcgagtacga 28620 ctgctacgtc aacggccgcc tcctcatcga gatgcgcgat ggctgcgccg gcttcttcac 28680 caacgaggag ctcgacgccg gcaagggcgt cgtcttcacc cgcggcgacc tcgccgcccg 28740 cgccaagatc ccaaagcagg acgtctcccc ctacgccgtc gccccctgcc tccacaagac 28800 caagctcaac gaaaaggaga tgcagaccct cgtcgacaag gactgggcat ccgtctttgg 28860 ctccaagaac ggcatgccgg aaatcaacta caaactctgc gcgcgtaaga tgctcatgat 28920 tgaccgcgtc accagcattg accacaaggg cggtgtctac ggcctcggtc agctcgtcgg 28980 tgaaaagatc ctcgagcgcg accactggta ctttccctgc cactttgtca aggatcaggt 29040 catggccgga tccctcgtct ccgacggctg cagccagatg ctcaagatgt acatgatctg 29100 gctcggcctc cacctcacca ccggaccctt tgacttccgc ccggtcaacg gccaccccaa 29160 caaggtccgc tgccgcggcc aaatctcccc gcacaagggc aagctcgtct acgtcatgga 29220 gatcaaggag atgggcttcg acgaggacaa cgacccgtac gccattgccg acgtcaacat 29280 cattgatgtc gacttcgaaa agggccagga ctttagcctc gaccgcatca gcgactacgg 29340 caagggcgac ctcaacaaga agatcgtcgt cgactttaag ggcatcgctc tcaagatgca 29400 gaagcgctcc accaacaaga acccctccaa ggttcagccc gtctttgcca acggcgccgc 29460 cactgtcggc cccgaggcct ccaaggcttc ctccggcgcc agcgccagcg ccagcgccgc 29520 cccggccaag cctgccttca gcgccgatgt tcttgcgccc aagcccgttg cccttcccga 29580 gcacatcctc aagggcgacg ccctcgcccc caaggagatg tcctggcacc ccatggcccg 29640 catcccgggc aacccgacgc cctcttttgc gccctcggcc tacaagccgc gcaacatcgc 29700 ctttacgccc ttccccggca accccaacga taacgaccac accccgggca agatgccgct 29760 cacctggttc aacatggccg agttcatggc cggcaaggtc agcatgtgcc tcggccccga 29820 gttcgccaag ttcgacgact cgaacaccag ccgcagcccc gcttgggacc tcgctctcgt 29880 cacccgcgcc gtgtctgtgt ctgacctcaa gcacgtcaac taccgcaaca tcgacctcga 29940 cccctccaag ggtaccatgg tcggcgagtt cgactgcccc gcggacgcct ggttctacaa 30000 gggcgcctgc aacgatgccc acatgccgta ctcgatcctc atggagatcg ccctccagac 30060 ctcgggtgtg ctcacctcgg tgctcaaggc gcccctgacc atggagaagg acgacatcct 30120 cttccgcaac ctcgacgcca acgccgagtt cgtgcgcgcc gacctcgact accgcggcaa 30180 gactatccgc aacgtcacca agtgcactgg ctacagcatg ctcggcgaga tgggcgtcca 30240 ccgcttcacc tttgagctct acgtcgatga tgtgctcttt tacaagggct cgacctcgtt 30300 cggctggttc gtgcccgagg tctttgccgc ccaggccggc ctcgacaacg gccgcaagtc 30360 ggagccctgg ttcattgaga acaaggttcc ggcctcgcag gtctcctcct ttgacgtgcg 30420 ccccaacggc agcggccgca ccgccatctt cgccaacgcc cccagcggcg cccagctcaa 30480 ccgccgcacg gaccagggcc agtacctcga cgccgtcgac attgtctccg gcagcggcaa 30540 gaagagcctc ggctacgccc acggttccaa gacggtcaac ccgaacgact ggttcttctc 30600 gtgccacttt tggtttgact cggtcatgcc cggaagtctc ggtgtcgagt ccatgttcca 30660 gctcgtcgag gccatcgccg cccacgagga tctcgctggc aagcacggca ttgccaaccc 30720 cacctttgtg cacgccccgg gcaagatcag ctggaagtac cgcggccagc tcacgcccaa 30780 gagcaagaag atggactcgg aggtccacat cgtgtccgtg gacgcccacg acggcgttgt 30840 cgacctcgtc gccgacggct tcctctgggc cgacagcctc cgcgtctact cggtgagcaa 30900 cattcgcgtg cgcatcgcct ccggtgaggc ccctgccgcc gcctcctccg ccgcctctgt 30960 gggctcctcg gcttcgtccg tcgagcgcac gcgctcgagc cccgctgtcg cctccggccc 31020 ggcccagacc atcgacctca agcagctcaa gaccgagctc ctcgagctcg atgccccgct 31080 ctacctctcg caggacccga ccagcggcca gctcaagaag cacaccgacg tggcctccgg 31140 ccaggccacc atcgtgcagc cctgcacgct cggcgacctc ggtgaccgct ccttcatgga 31200 gacctacggc gtcgtcgccc cgctgtacac gggcgccatg gccaagggca ttgcctcggc 31260 ggacctcgtc atcgccgccg gcaagcgcaa gatcctcggc tcctttggcg ccggcggcct 31320 ccccatgcac cacgtgcgcg ccgccctcga gaagatccag gccgccctgc ctcagggccc 31380 ctacgccgtc aacctcatcc actcgccttt tgacagcaac ctcgagaagg gcaacgtcga 31440 tctcttcctc gagaagggcg tcactgtggt ggaggcctcg gcattcatga ccctcacccc 31500 gcaggtcgtg cgctaccgcg ccgccggcct ctcgcgcaac gccgacggtt cggtcaacat 31560 ccgcaaccgc atcatcggca aggtctcgcg caccgagctc gccgagatgt tcatccgccc 31620 ggccccggag cacctcctcg agaagctcat cgcctcgggc gagatcaccc aggagcaggc 31680 cgagctcgcg cgccgcgttc ccgtcgccga cgatatcgct gtcgaggctg actcgggcgg 31740 ccacaccgac aaccgcccca tccacgtcat cctcccgctc atcatcaacc tccgcaaccg 31800 cctgcaccgc gagtgcggct accccgcgca cctccgcgtc cgcgttggcg ccggcggtgg 31860 cgtcggctgc ccgcaggccg ccgccgccgc gctcaccatg ggcgccgcct tcatcgtcac 31920 cggcactgtc aaccaggtcg ccaagcagtc cggcacctgc gacaacgtgc gcaagcagct 31980 ctcgcaggcc acctactcgg atatctgcat ggccccggcc gccgacatgt tcgaggaggg 32040 cgtcaagctc caggtcctca agaagggaac catgttcccc tcgcgcgcca acaagctcta 32100 cgagctcttt tgcaagtacg actccttcga ctccatgcct cctgccgagc tcgagcgcat 32160 cgagaagcgt atcttcaagc gcgcactcca ggaggtctgg gaggagacca aggactttta 32220 cattaacggt ctcaagaacc cggagaagat ccagcgcgcc gagcacgacc ccaagctcaa 32280 gatgtcgctc tgcttccgct ggtaccttgg tcttgccagc cgctgggcca acatgggcgc 32340 cccggaccgc gtcatggact accaggtctg gtgtggcccg gccattggcg ccttcaacga 32400 cttcatcaag ggcacctacc tcgaccccgc tgtctccaac gagtacccct gtgtcgtcca 32460 gatcaacctg caaatcctcc gtggtgcctg ctacctgcgc cgtctcaacg ccctgcgcaa 32520 cgacccgcgc attgacctcg agaccgagga tgctgccttt gtctacgagc ccaccaacgc 32580 gctctaagct agcatttaaa tctaggccat ggtgacttaa ggtagttagc ttaatcactt 32640 aggtcaccca gtccaagccc ggcctagcac gatgatcatg agtgttgcta ttgatgtatg 32700 ttaatactat acacagtcat cgtgtgtgtt ttataaaact gtaagcttag gatgtttgag 32760 gctaatgtaa ttagcactac accgtaataa atgagaggtt tactttatgt tttaataatg 32820 ttcttaccct tgctgctctc taaactgtaa tggcgtacgg ttagctaaac taaagtaaaa 32880 atcgattcgc ttgagtaaaa ttagtgaccg ggaaagatca tcgactacta ctgctcagag 32940 atgtaatcgg taattaagac ttcaagctca ataattaatt aggagtaaat ctggcttcga 33000 atttttaaat tgtcaacttc caaagaagga ttggcaagac tcaatctcaa ttgcccaaca 33060 aacatatatg gctctttaca aatttggaat ctctttttga tggcaagaac gtcgtaattc 33120 ctctgtcgat ctcatgtaat taccaaactc atctacttat tactttcttt atatgcactt 33180 actgatgcgg ccgcttaatt aatcacgagt ggatgactgt cacgactgac tcatgactga 33240 ctactagtgc tagcctcgag gtcgacacaa ctttgtataa taaagttgtc tagacctagg 33300 cgagtcatga gtcgtgacat gacgtcatgc tcatcagact gcggccgcag cttatagtaa 33360 agattttttt gttaataaaa tgaaagttaa tatttatgga tttatttgaa catatttaca 33420 agtttttgtt aataaattta tttacagaag tgttaaattt gaaaatgtgt taaatgagag 33480 ttaatattta tggatataat attactttat ttaaaaataa aatgaattta tattaaaata 33540 tgatttggta aaagatatta taaagaggaa cttggatgag aaagacgacg atgggcaaaa 33600 gttgcatggc acaccgcttt tgttcagtaa catctgcact gtggcgcctt tttcacactc 33660 accatgcatg ctgccacgtc agctttcaca ctatgacacc actggccata cacgttgcca 33720 cctcagcttt ctcctcttct cactatgaca cgactggcca tgcatgcatg catgctgcca 33780 cctcagctcc cgcctcttca cccgtgtctt tctctcatgt cgcagtgctg cgtgccaacc 33840 tcttctctct ataaatagag agaagagaat gatggttaaa ctcaaaccaa aacaagaaaa 33900 catacacaaa tagcaaaacg gatccacaac caacctacca tggctcccac tcccgacgcc 33960 accgcgcctc tgaacaagcc gagcgactat gccgtctacc acgaggaaga cggccccttc 34020 tggaccgccg attccagcgg cgtctcgcgc gtgaacttta gcgagaccgg cgtgggatcc 34080 gagggcgtca tccctgcgct cacgctcatc gacgtcttcg agagggccgt caagcgcggc 34140 ggaaacagga tcgccttccg cacggaaaac atgcccacgc tccgccgcgg cgaagaggcc 34200 ccggacgcgc tgccgctcaa ggactggaag tcctggtcct ggaaacagta caaggccgac 34260 gtccaccgca tcgccagggc tctcatggac ctcggcgttg agcagcatga cgccgtctcc 34320 atttttggct ttaactcgcc cgaatggttt ctcagtgccg tcggcgccgt gcacgcaggt 34380 gccaagattg ccggcattta cccctcagac acgcccgccc aggtccagta caaggccttc 34440 cacagtgaca ccgctgttgc cgttgtcgaa aacgagcagt gcttcaagaa gttcgccgag 34500 gtcgtcgagg accttcctta cctcaaggcc attgtttgct gggactatga agccacagac 34560 atcacgcgcg aggacggctc cgtcgtcgag gtcctcacct ttgccgagtt cctcaagcgc 34620 ggcgacaccg tcgaggcggc cgcccttgac gagcgcatct ccaagatcga gcccaccatg 34680 tgcgctgccc ttatttacac cagcggtact accggccgcc ccaaggccgt tatgatttcg 34740 cacgacaacc ttgttttcga ggccagcgcc gtcgtcccca acctcggagg agcctgtacg 34800 accactgctg aggagcgcat tctctcgtac ctgcctctct cgcacgtcgc tggtatgatg 34860 gttgatatta ttgcccccat cattgccacc gccttccaca agggccgcat ctgcgtctgc 34920 tttgctcgcc cgtacgattt gcgcaccggc acgctgggcc agcgcctcaa cgccgtggag 34980 cccaccatct tccttggcgt gccccgtgtg tgggaaaaga ttcaggaaaa gctcatggcc 35040 gtcggtgcca aaaccaccgg cctcaagaag aagctctcta cggccgccaa gaagcgtggt 35100 cttgaattcc aggaggagca gcaaatcggc cgctccggtg ccaaccctgg ctttggcccc 35160 cttggcatct acaagaagct cctcggcctc atcaagggca agctgggcct caccaagtgc 35220 aagtttgcct ttgctggtgc cgcgcccatg acccgtgaga cccttcagta ctttggcgcg 35280 ctgaacatca acattaacga ggtctacggc atgtccgagt gctccggtgc cgccacctgg 35340 tccacggaca aggcccacga gtggggcact gttggctacg agatgcccag ttgcgaggtc 35400 cgcgtcttca agattgccga ggacggtacc aagaccgagt gcccgcgcgc cgccgacatt 35460 atgcatgcta ccgaggagga gcagggcgaa gtttgcttcc gcggccgtaa catcatgatg 35520 ggctaccttg ccaaccccaa gcttggcgac gaccacgttg ccgagatcga ggagaagaac 35580 gctgccgcta tcgactccga gggctggctc cacagtggtg ataagggcgc catttctacc 35640 cgcggcatgc tcaagatcac gggccgctac aaggagctca tcatcggcgc cggtggcgag 35700 aacgtggcgc ccgtccctat tgaggacgcc atcaaggcgc gcatgccttt tgtttccaac 35760 gccatgatgg tcggagataa gcgcaagttc atggctgtcc tccttaccct caagacggtt 35820 ggcgccacgg gcgagcttcc cggtacgaac aagctcatgg gcgctgccgc cgactatggt 35880 gagaccatcg aggacgcctg cgacaacgag gcgctcattg aggagatcac gcagcagctc 35940 aaggagatcg gtgatgatgg cgatgtcacg ccctcgaacg cggctcgcat ccaaaagttc 36000 accattctcc cgctcgactt ttccgtctcc acggacgagc tcacggccac gctcaagctc 36060 aagcgctccg tggtcgcaga caagtacgaa gacatcatcg aggcctttta cgagtccaag 36120 agcgtttttg tgccgtactc gaccgttggc gcctacgcca cgggcggccc ggtcgacgac 36180 tccgttgtcg atggctcctt caagggcgac tttagcatga ttggcgacga tgatccggat 36240 cttcaaaacg tcgatgtcct cgaggcgatt gacgaggaca attaaatttc cagtccaagc 36300 ccggcctagc acgatgatca tgagtgttgc tattgatgta tgttaatact atacacagtc 36360 atcgtgtgtg ttttataaaa ctgtaagctt aggatgtttg aggctaatgt aattagcact 36420 acaccgtaat aaatgagagg tttactttat gttttaataa tgttcttacc cttgctgctc 36480 tctaaactgt aatggcgtac ggttagctaa actaaagtaa aaatcgattc gcttgagtaa 36540 aattagtgac cgggaaagat catcgactac tactgctcag agatgtaatc ggtaattaag 36600 acttcaagct caataattaa ttaggagtaa atctggcttc gaatttttaa attgtcaact 36660 tccaaagaag gattggcaag actcaatctc aattgcccaa caaacatatg gctctttaca 36720 aatttggaat ctctttttga tggcaagaac gtcgtaattc ctctgtcgat ctcatgtaat 36780 taccaaactc atctacttat tactttcttt atatgcactt actgatgcgg ccgcttaatt 36840 aatctgatca tggatgctac gtcacggcag tacaggacta tcactagagt cactggtcac 36900 atcagcggcc gcagcttata gtaaagattt ttttgttaat aaaatgaaag ttaatattta 36960 tggatttatt tgaacatatt tacaagtttt tgttaataaa tttatttaca gaagtgttaa 37020 atttgaaaat gtgttaaatg agagttaata tttatggata taatattact ttatttaaaa 37080 ataaaatgaa tttatattaa aatatgattt ggtaaaagat attataaaga ggaacttgga 37140 tgagaaagac gacgatgggc aaaagttgca tggcacaccg cttttgttca gtaacatctg 37200 cactgtggcg cctttttcac actcaccatg catgctgcca cgtcagcttt cacactatga 37260 caccactggc catacacgtt gccacctcag ctttctcctc ttctcactat gacacgactg 37320 gccatgcatg catgcatgct gccacctcag ctcccgcctc ttcacccgtg tctttctctc 37380 atgtcgcagt gctgcgtgcc aacctcttct ctctataaat agagagaaga gaatgatggt 37440 taaactcaaa ccaaaacaag aaaacataca caaatagcaa aacggatcca caaccaaaaa 37500 tgttgcagca tacttggcta ccaaaacccc caaatttaac cttattgtca gatgaagttc 37560 atctctggcg cattcccctt gaccaaccag aatcacagct acaggattta gccgctacct 37620 tatctagtga cgaattagcc cgtgcaaaca gattttattt tcccgaacat cgccggcgtt 37680 ttactgctgg tcgtggtatt ctccgcagta tcttgggggg ctatttgggt gtggaaccag 37740 ggcaagttaa atttgattat gaatcccgtg gtaaaccaat attaggcgat cgctttgccg 37800 agagtggttt attatttaac ttgtcacact cccagaactt ggccttgtgt gcagtcaatt 37860 acacgcgcca aatcggcatc gatttagaat atctccgccc cacatctgat ttagaatccc 37920 ttgccaaaag gttcttttta ccgcgagaat atgaattatt gcgatcgcta cccgatgagc 37980 aaaaacaaaa aattttcttt cgttactgga cttgtaaaga ggcttatctt aaagcaacgg 38040 gtgacggcat cgctaaatta gaggaaattg aaatagcact aactcccaca gaaccagcta 38100 agttacagac agctccagcg tggagtctcc tagagctagt gccagatgat aattgtgttg 38160 ctgctgttgc cgtggcgggt tttggctggc agccaaaatt ctggcattat tgagtagtta 38220 gcttaatcac ttaggtcacc cagtccaagc ccggcctagc acgatgatca tgagtgttgc 38280 tattgatgta tgttaatact atacacagtc atcgtgtgtg ttttataaaa ctgtaagctt 38340 aggatgtttg aggctaatgt aattagcact acaccgtaat aaatgagagg tttactttat 38400 gttttaataa tgttcttacc cttgctgctc tctaaactgt aatggcgtac ggttagctaa 38460 actaaagtaa aaatcgattc gcttgagtaa aattagtgac cgggaaagat catcgactac 38520 tactgctcag agatgtaatc ggtaattaag acttcaagct caataattaa ttaggagtaa 38580 atctggcttc gaatttttaa attgtcaact tccaaagaag gattggcaag actcaatctc 38640 aattgcccaa caaacatatg gctctttaca aatttggaat ctctttttga tggcaagaac 38700 gtcgtaattc ctctgtcgat ctcatgtaat taccaaactc atctacttat tactttcttt 38760 atatgcactt actgatgcct agtgctagcc tcgaggtcga cacccagctt t 38811 <210> 40 <211> 35302 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7368 <400> 40 ttgtacaaag tggttgcggc cgcttaatta aatttaaatt caattaatgc aatcttgatt 60 ttcaacaacg aaggtaatgg cgtaaaagaa aaaatgtatg ttattgtatt gatctttcat 120 gatgttgaag cgtgccataa tatgatgatg tataattaaa atattaactg tcgcatttta 180 ttgaaatggc actgttattt caaccatatc tttgattctg ttacatgaca cgactgcaag 240 aagtaaataa tagacgccgt tgttaaagaa ttgctatcat atgtgcctaa ctagagggaa 300 tttgagcgtc agacctaatc aaatattaca aaatatctca ctctgtcgcc agcaatggtg 360 taatcagcgc agacaaatgg cgtaaagatc gcggaaaaac ctccccgagt ggcatgatag 420 ctgcctctgt attgctgatt tagtcagcct tatttgactt aagggtgccc tcgttagtga 480 caaattgctt tcaaggagac agccatgccc cacactttgt tgaaaaacaa attgcctttg 540 gggagacggt aaagccagtt gctcttcaat aaggaatgtc gaggaggcaa tgtaaccgcc 600 tctggtagta cacttctcta atccaaaaat caatttgtat tcaagatacc gcaaaaaact 660 tatggtttaa accctgcagg actagtccag aaggtaatta tccaagatgt agcatcaaga 720 atccaatgtt tacgggaaaa actatggaag tattatgtaa gctcagcaag aagcagatca 780 atatgcggca catatgcaac ctatgttcaa aaatgaagaa tgtacagata caagatccta 840 tactgccaga atacgaagaa gaatacgtag aaattgaaaa agaagaacca ggcgaagaaa 900 agaatcttga agacgtaagc actgacgaca acaatgaaaa gaagaagata aggtcggtga 960 ttgtgaaaga gacatagagg acacatgtaa ggtggaaaat gtaagggcgg aaagtaacct 1020 tatcacaaag gaatcttatc ccccactact tatcctttta tatttttccg tgtcattttt 1080 gcccttgagt tttcctatat aaggaaccaa gttcggcatt tgtgaaaaca agaaaaaatt 1140 tggtgtaagc tattttcttt gaagtactga ggatacaact tcagagaaat ttgtaagttt 1200 gtaggtacca gatctggatc ccaaaccatg tctccggaga ggagaccagt tgagattagg 1260 ccagctacag cagctgatat ggccgcggtt tgtgatatcg ttaaccatta cattgagacg 1320 tctacagtga actttaggac agagccacaa acaccacaag agtggattga tgatctagag 1380 aggttgcaag atagataccc ttggttggtt gctgaggttg agggtgttgt ggctggtatt 1440 gcttacgctg ggccctggaa ggctaggaac gcttacgatt ggacagttga gagtactgtt 1500 tacgtgtcac ataggcatca aaggttgggc ctaggatcta cattgtacac acatttgctt 1560 aagtctatgg aggcgcaagg ttttaagtct gtggttgctg ttataggcct tccaaacgat 1620 ccatctgtta ggttgcatga ggctttggga tacacagccc ggggtacatt gcgcgcagct 1680 ggatacaagc atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca 1740 gctcctccaa ggccagttag gccagttacc caaatctgag tagttagctt aatcacctag 1800 agctcgatcg gcggcaatag cttcttagcg ccatcccggg ttgatcctat ctgtgttgaa 1860 atagttgcgg tgggcaaggc tctctttcag aaagacaggc ggccaaagga acccaaggtg 1920 aggtgggcta tggctctcag ttccttgtgg aagcgcttgg tctaaggtgc agaggtgtta 1980 gcgggatgaa gcaaaagtgt ccgattgtaa caagatatgt tgatcctacg taaggatatt 2040 aaagtatgta ttcatcacta atataatcag tgtattccaa tatgtactac gatttccaat 2100 gtctttattg tcgccgtatg taatcggcgt cacaaaataa tccccggtga ctttctttta 2160 atccaggatg aaataatatg ttattataat ttttgcgatt tggtccgtta taggaattga 2220 agtgtgcttg aggtcggtcg ccaccactcc catttcataa ttttacatgt atttgaaaaa 2280 taaaaattta tggtattcaa tttaaacacg tatacttgta aagaatgata tcttgaaaga 2340 aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc caagcaaaaa 2400 cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat tatatcagct 2460 ggtacattgc cgtagatgaa agactgagtg cgatattatg gtgtaataca taggaattcg 2520 tttaaacgat ctgcgtctaa ttttcggtcc aacttgcaca ggaaagacgt cgaccgcggt 2580 agctcttgcc cagcagactg ggcttccagt cctttcgctc gatcgggtcc aatgttgtcc 2640 tcagctgtga accggaagcg gacgaccaac agtggaagaa ctgaaaggaa cgagccgtct 2700 ataccttgat gatcggcctc tggtgaaggg tatcatcgca gccaagcaag ctcatgaaag 2760 gctgatgggg gaggtgtata attatgaggc ccacggcggg cttattcttt agggaggatc 2820 tatctcgttg ctcaagtgca tggcgcaaag cagttattgg agtgcggatt ttcgttggca 2880 tattattcgc cacgagttag cagacgaaga gaccttcatg aacgtggcca aggccagagt 2940 taagcagatg ttacgccctg ctgcaggcct ttctattatc caatagttgg ttgatctttg 3000 gaaagagcct cggctgaggc ccatactgaa agagatcgat ggatatcgat atgccatgtt 3060 gtttgctagc cagaaccaga tcacatccga tatgctattg cagcttgacg cagatatgga 3120 ggataagttg attcatggga tcgctcagga gtagctcatc catgcacgcc gacaagaaca 3180 gaaattccgt cgagttaacg cagccgctta cgacggattc gaaggtcatc cattcggaat 3240 gtattagttt gcaccagctc cgcgtcacac ctgtcttcat ttgaataaga tgttagcaat 3300 tgtttttagc tttgtcttgt tgtggcaggg cggcaagtgc ttcagacatc attctgtttt 3360 caaattttat gctggagaac agcttcttaa ttcctttgga aataatagac tgcgtcttaa 3420 aattcagatg tctggatata gatatgattg taaaataacc tatttaagtg tcatttagaa 3480 cataagtttt atgaatgttc ttccattttc gtcatcgaac gaataagagt aaatacacct 3540 tttttaacat tacaaataag ttcttatacg ttgtttatac accgggaatc atttccatta 3600 ttttcgcgca aaagtcacgg atattcgtga aagcgacata aactgcgaaa tttgcgggga 3660 gtgtcttgag tttgcctcga ggctagcgca tgcacataga cacacacatc atctcattga 3720 tgcttggtaa taattgtcat tagattgttt ttatgcatag atgcactcga aatcagccaa 3780 ttttagacaa gtatcaaacg gatgtgactt cagtacatta aaaacgtccg caatgtgtta 3840 ttaagttgtc taagcgtcaa tttgatttac aattgaatat atcctgcccc agccagccaa 3900 cagctcgatt tacaattgaa tatatcctgc cggccggccc acgcgtgtcg aggaattctg 3960 atctggcccc catttggacg tgaatgtaga cacgtcgaaa taaagatttc cgaattagaa 4020 taatttgttt attgctttcg cctataaata cgacggatcg taatttgtcg ttttatcaaa 4080 atgtactttc attttataat aacgctgcgg acatctacat ttttgaattg aaaaaaaatt 4140 ggtaattact ctttcttttt ctccatattg accatcatac tcattgctga tccatgtaga 4200 tttcccggac atgaagccat ttacaattga atatatcctg ccgccgctgc cgctttgcac 4260 ccggtggagc ttgcatgttg gtttctacgc agaactgagc cggttaggca gataatttcc 4320 attgagaact gagccatgtg caccttcccc ccaacacggt gagcgacggg gcaacggagt 4380 gatccacatg ggacttttaa acatcatccg tcggatggcg ttgcgagaga agcagtcgat 4440 ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg atcgcaaagt atttgaacgc 4500 aggtacaatc gagccgacgt tcacgcggaa cgaccaagca agcttggctg ccatttttgg 4560 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc 4620 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca 4680 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga 4740 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg 4800 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa 4860 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca 4920 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt 4980 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct 5040 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc 5100 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg 5160 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 5220 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat 5280 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 5340 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 5400 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 5460 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 5520 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc 5580 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg 5640 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg 5700 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc 5760 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg 5820 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca 5880 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg 5940 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg 6000 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg 6060 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc 6120 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga 6180 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg 6240 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa 6300 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc 6360 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt 6420 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg 6480 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag 6540 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc 6600 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc 6660 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc 6720 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac 6780 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc 6840 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac 6900 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac 6960 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc 7020 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc 7080 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg 7140 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc 7200 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc 7260 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg 7320 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct 7380 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc 7440 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattg 7500 tgattaaggc tcagattcga cggcttggag cggccgacgt gcaggatttc cgcgagatcc 7560 gattgtcggc cctgaagaaa gctccagaga tgttcgggtc cgtttacgag cacgaggaga 7620 aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca 7680 tcgacggcga gatcattggg ctgtcggtct tcaaacagga ggacggcccc aaggacgctc 7740 acaaggcgca tctgtccggc gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg 7800 gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt gatgatcgtc cgacagattc 7860 caacgggaat ctggtggatg cgcatcttca tcctcggcgc acttaatatt tcgctattct 7920 ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg 7980 ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct gctaggtagc ccgatacgat 8040 tgatggcggt cctgggggct atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac 8100 caaacgcagc gctagatcct gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg 8160 cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt gcctctgctc acctttaccg 8220 cctggcaact ggcggccgga ggacttctgc tcgttccagt agctttagtg tttgatccgc 8280 caatcccgat gcctacagga accaatgttc tcggcctggc gtggctcggc ctgatcggag 8340 cgggtttaac ctacttcctt tggttccggg ggatctcgcg actcgaacct acagttgttt 8400 ccttactggg ctttctcagc ccccgagcgc ttagtgggaa tttgtacccc ttatcgaacc 8460 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt 8520 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 8580 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 8640 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 8700 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 8760 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 8820 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 8880 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 8940 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 9000 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 9060 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 9120 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 9180 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca 9240 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg 9300 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt 9360 cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa 9420 ggtggttcta agcctcgtct tgcgatggca tttcgatcca ttcccattcc gcgctcaaga 9480 tggcttcccc tcggcagttc atcagggcta aatcaatcta gccgacttgt ccggtgaaat 9540 gggctgcact ccaacagaaa caatcaaaca aacatacaca gcgacttatt cacacgagct 9600 caaattacaa cggtatatat cctgccagtc agcatcatca caccaaaagt taggcccgaa 9660 tagtttgaaa ttagaaagct cgcaattgag gtctacaggc caaattcgct cttagccgta 9720 caatattact caccggatcc taaccggttt aattaaggcg cgccatgccc gggcaagcgg 9780 ccgcacaagt ttgtacaaaa aagcaggctt ctagacctag ctaggtggag tcatcacgca 9840 gactatctca gcatgtgcgt agcacgcggc cgcagcttat agtaaagatt tttttgttaa 9900 taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt ttgttaataa 9960 atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat atttatggat 10020 ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt tggtaaaaga 10080 tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc atggcacacc 10140 gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat gcatgctgcc 10200 acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca gctttctcct 10260 cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca gctcccgcct 10320 cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc tctctataaa 10380 tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac acaaatagca 10440 aaacggatcc acaaccaaaa atggcggccc gtctgcagga gcaaaaggga ggcgagatgg 10500 atacccgcat tgccatcatc ggcatgtcgg ccatcctccc ctgcggcacg accgtgcgcg 10560 agtcgtggga gaccatccgc gccggcatcg actgcctgtc ggatctcccc gaggaccgcg 10620 tcgacgtgac ggcgtacttt gaccccgtca agaccaccaa ggacaagatc tactgcaagc 10680 gcggtggctt cattcccgag tacgactttg acgcccgcga gttcggactc aacatgttcc 10740 agatggagga ctcggacgca aaccagacca tctcgcttct caaggtcaag gaggccctcc 10800 aggacgccgg catcgacgcc ctcggcaagg aaaagaagaa catcggctgc gtgctcggca 10860 ttggcggcgg ccaaaagtcc agccacgagt tctactcgcg ccttaattat gttgtcgtgg 10920 agaaggtcct ccgcaagatg ggcatgcccg aggaggacgt caaggtcgcc gtcgaaaagt 10980 acaaggccaa cttccccgag tggcgcctcg actccttccc tggcttcctc ggcaacgtca 11040 ccgccggtcg ctgcaccaac accttcaacc tcgacggcat gaactgcgtt gtcgacgccg 11100 catgcgcctc gtccctcatc gccgtcaagg tcgccatcga cgagctgctc tacggtgact 11160 gcgacatgat ggtcaccggt gccacctgca cggataactc catcggcatg tacatggcct 11220 tctccaagac ccccgtgttc tccacggacc ccagcgtgcg cgcctacgac gaaaagacaa 11280 agggcatgct catcggcgag ggctccgcca tgctcgtcct caagcgctac gccgacgccg 11340 tccgcgacgg cgatgagatc cacgctgtta ttcgcggctg cgcctcctcc agtgatggca 11400 aggccgccgg catctacacg cccaccattt cgggccagga ggaggccctc cgccgcgcct 11460 acaaccgcgc ctgtgtcgac ccggccaccg tcactctcgt cgagggtcac ggcaccggta 11520 ctcccgttgg cgaccgcatc gagctcaccg ccttgcgcaa cctctttgac aaggcctacg 11580 gcgagggcaa caccgaaaag gtcgctgtgg gcagcatcaa gtccagcatc ggccatctca 11640 aggccgtcgc cggtctcgcc ggtatgatca aggtcatcat ggcgctcaag cacaagactc 11700 tcccgggcac catcaacgtc gacaacccac ccaacctcta cgacaacacg cccatcaacg 11760 agtcctcgct ctacattaac accatgaacc gcccctggtt cccgccccct ggtgtgcccc 11820 gccgcgccgg catttcgagc tttggctttg gtggcgccaa ctaccacgcc gtcctcgagg 11880 aggccgagcc cgagcacacg accgcgtacc gcctcaacaa gcgcccgcag cccgtgctca 11940 tgatggccgc cacgcccgcg gccctccagt cgctctgcga ggcccagctc aaggagttcg 12000 aggccgccat caaggagaac gagaccgtca agaacaccgc ctacatcaag tgcgtcaagt 12060 tcggcgagca gttcaaattc cctggctcca tcccggccac aaacgcgcgc ctcggcttcc 12120 tcgtcaagga tgctgaggat gcctgctcca ccctccgtgc catctgcgcc caattcgcca 12180 aggatgtcac caaggaggcc tggcgcctcc cccgcgaggg cgtcagcttc cgcgccaagg 12240 gcatcgccac caacggcgct gtcgccgcgc tcttctccgg ccagggcgcg cagtacacgc 12300 acatgtttag cgaggtggcc atgaactggc cccagttccg ccagagcatt gccgccatgg 12360 acgccgccca gtccaaggtc gctggaagcg acaaggactt tgagcgcgtc tcccaggtcc 12420 tctacccgcg caagccgtac gagcgtgagc ccgagcagga ccacaagaag atctccctca 12480 ccgcctactc gcagccctcg accctggcct gcgctctcgg tgcctttgag atcttcaagg 12540 aggccggctt caccccggac tttgccgccg gccattcgct cggtgagttc gccgccctct 12600 acgccgcggg ctgcgtcgac cgcgacgagc tctttgagct tgtctgccgc cgcgcccgca 12660 tcatgggcgg caaggacgca ccggccaccc ccaagggctg catggccgcc gtcattggcc 12720 ccaacgccga gaacatcaag gtccaggccg ccaacgtctg gctcggcaac tccaactcgc 12780 cttcgcagac cgtcatcacc ggctccgtcg aaggtatcca ggccgagagc gcccgcctcc 12840 agaaggaggg cttccgcgtc gtgcctcttg cctgcgagag cgccttccac tcgccccaga 12900 tggagaacgc ctcgtcggcc ttcaaggacg tcatctccaa ggtctccttc cgcaccccca 12960 aggccgagac caagctcttc agcaacgtct ctggcgagac ctaccccacg gacgcccgcg 13020 agatgcttac gcagcacatg accagcagcg tcaagttcct cacccaggtc cgcaacatgc 13080 accaggccgg tgcgcgcatc tttgtcgagt tcggacccaa gcaggtgctc tccaagcttg 13140 tctccgagac cctcaaggat gacccctcgg ttgtcaccgt ctctgtcaac ccggcctcgg 13200 gcacggattc ggacatccag ctccgcgacg cggccgtcca gctcgttgtc gctggcgtca 13260 accttcaggg ctttgacaag tgggacgccc ccgatgccac ccgcatgcag gccatcaaga 13320 agaagcgcac taccctccgc ctttcggccg ccacctacgt ctcggacaag accaagaagg 13380 tccgcgacgc cgccatgaac gatggccgct gcgtcaccta cctcaagggc gccgcaccgc 13440 tcatcaaggc cccggagccc gttgtcgacg aggccgccaa gcgcgaggcc gagcgtctcc 13500 agaaggagct tcaggatgcc cagcgccagc tcgacgacgc caagcgcgcc gccgccgagg 13560 ccaactccaa gctcgccgct gccaaggagg aggccaagac cgccgctgct tcggccaagc 13620 ccgcagttga cactgctgtt gtcgaaaagc atcgtgccat cctcaagtcc atgctcgcgg 13680 agctcgatgg ctacggatcg gtcgacgctt cttccctcca gcagcagcag cagcagcaga 13740 cggcccccgc cccggtcaag gctgctgcgc ctgccgcccc cgttgcctcg gcccctgccc 13800 cggctgtctc gaacgagctt cttgagaagg ccgagactgt cgtcatggag gtcctcgccg 13860 ccaagaccgg ctacgagacc gacatgatcg aggctgacat ggagctcgag accgagctcg 13920 gcattgactc catcaagcgt gtcgagatcc tctccgaggt ccaggccatg ctcaatgtcg 13980 aggccaagga tgtcgatgcc ctcagccgca ctcgcactgt tggtgaggtt gtcaacgcca 14040 tgaaggccga gatcgctggc agctctgccc cggcgcctgc tgccgctgct ccggctccgg 14100 ccaaggctgc ccctgccgcc gctgcgcctg ctgtctcgaa cgagcttctc gagaaggccg 14160 agaccgtcgt catggaggtc ctcgccgcca agactggcta cgagactgac atgatcgagt 14220 ccgacatgga gctcgagact gagctcggca ttgactccat caagcgtgtc gagatcctct 14280 ccgaggttca ggccatgctc aacgtcgagg ccaaggacgt cgacgctctc agccgcactc 14340 gcactgtggg tgaggtcgtc aacgccatga aggctgagat cgctggtggc tctgccccgg 14400 cgcctgccgc cgctgcccca ggtccggctg ctgccgcccc tgcgcctgcc gccgccgccc 14460 ctgctgtctc gaacgagctt cttgagaagg ccgagaccgt cgtcatggag gtcctcgccg 14520 ccaagactgg ctacgagact gacatgatcg agtccgacat ggagctcgag accgagctcg 14580 gcattgactc catcaagcgt gtcgagattc tctccgaggt ccaggccatg ctcaacgtcg 14640 aggccaagga cgtcgacgct ctcagccgca cccgcactgt tggcgaggtc gtcgatgcca 14700 tgaaggccga gatcgctggt ggctctgccc cggcgcctgc cgccgctgct cctgctccgg 14760 ctgctgccgc ccctgcgcct gccgcccctg cgcctgctgt ctcgagcgag cttctcgaga 14820 aggccgagac tgtcgtcatg gaggtcctcg ccgccaagac tggctacgag actgacatga 14880 tcgagtccga catggagctc gagaccgagc tcggcattga ctccatcaag cgtgtcgaga 14940 ttctctccga ggtccaggcc atgctcaacg tcgaggccaa ggacgtcgac gctctcagcc 15000 gcacccgcac tgttggcgag gtcgtcgatg ccatgaaggc cgagatcgct ggtggctctg 15060 ccccggcgcc tgccgccgct gctcctgctc cggctgctgc cgcccctgcg cctgccgccc 15120 ctgcgcctgc cgcccctgcg cctgctgtct cgagcgagct tctcgagaag gccgagactg 15180 tcgtcatgga ggtcctcgcc gccaagactg gctacgagac tgacatgatt gagtccgaca 15240 tggagctcga gaccgagctc ggcattgact ccatcaagcg tgtcgagatt ctctccgagg 15300 ttcaggccat gctcaacgtc gaggccaagg acgtcgacgc tctcagccgc actcgcactg 15360 ttggtgaggt cgtcgatgcc atgaaggctg agatcgctgg cagctccgcc tcggcgcctg 15420 ccgccgctgc tcctgctccg gctgctgccg ctcctgcgcc cgctgccgcc gcccctgctg 15480 tctcgaacga gcttctcgag aaagccgaga ctgtcgtcat ggaggtcctc gccgccaaga 15540 ctggctacga gactgacatg atcgagtccg acatggagct cgagactgag ctcggcattg 15600 actccatcaa gcgtgtcgag atcctctccg aggttcaggc catgctcaac gtcgaggcca 15660 aggacgtcga tgccctcagc cgcacccgca ctgttggcga ggttgtcgat gccatgaagg 15720 ccgagatcgc tggtggctct gccccggcgc ctgccgccgc tgcccctgct ccggctgccg 15780 ccgcccctgc tgtctcgaac gagcttctcg agaaggccga gactgtcgtc atggaggtcc 15840 tcgccgccaa gactggctac gagaccgaca tgatcgagtc cgacatggag ctcgagaccg 15900 agctcggcat tgactccatc aagcgtgtcg agattctctc cgaggttcag gccatgctca 15960 acgtcgaggc caaggacgtc gatgctctca gccgcactcg cactgttggc gaggtcgtcg 16020 atgccatgaa ggctgagatc gccggcagct ccgccccggc gcctgccgcc gctgctcctg 16080 ctccggctgc tgccgctcct gcgcccgctg ccgctgcccc tgctgtctcg agcgagcttc 16140 tcgagaaggc cgagaccgtc gtcatggagg tcctcgccgc caagactggc tacgagactg 16200 acatgattga gtccgacatg gagctcgaga ctgagctcgg cattgactcc atcaagcgtg 16260 tcgagatcct ctccgaggtt caggccatgc tcaacgtcga ggccaaggac gtcgatgccc 16320 tcagccgcac ccgcactgtt ggcgaggttg tcgatgccat gaaggccgag atcgctggtg 16380 gctctgcccc ggcgcctgcc gccgctgccc ctgctccggc tgccgccgcc cctgctgtct 16440 cgaacgagct tcttgagaag gccgagaccg tcgtcatgga ggtcctcgcc gccaagactg 16500 gctacgagac cgacatgatc gagtccgaca tggagctcga gaccgagctc ggcattgact 16560 ccatcaagcg tgtcgagatt ctctccgagg ttcaggccat gctcaacgtc gaggccaagg 16620 acgtcgacgc tctcagccgc actcgcactg ttggcgaggt cgtcgatgcc atgaaggctg 16680 agatcgctgg tggctctgcc ccggcgcctg ccgccgctgc tcctgcctcg gctggcgccg 16740 cgcctgcggt caagattgac tcggtccacg gcgctgactg tgatgatctt tccctgatgc 16800 acgccaaggt ggttgacatc cgccgcccgg acgagctcat cctggagcgc cccgagaacc 16860 gccccgttct cgttgtcgat gacggcagcg agctcaccct cgccctggtc cgcgtcctcg 16920 gcgcctgcgc cgttgtcctg acctttgagg gtctccagct cgctcagcgc gctggtgccg 16980 ctgccatccg ccacgtgctc gccaaggatc tttccgcgga gagcgccgag aaggccatca 17040 aggaggccga gcagcgcttt ggcgctctcg gcggcttcat ctcgcagcag gcggagcgct 17100 tcgagcccgc cgaaatcctc ggcttcacgc tcatgtgcgc caagttcgcc aaggcttccc 17160 tctgcacggc tgtggctggc ggccgcccgg cctttatcgg tgtggcgcgc cttgacggcc 17220 gcctcggatt cacttcgcag ggcacttctg acgcgctcaa gcgtgcccag cgtggtgcca 17280 tctttggcct ctgcaagacc atcggcctcg agtggtccga gtctgacgtc ttttcccgcg 17340 gcgtggacat tgctcagggc atgcaccccg aggatgccgc cgtggcgatt gtgcgcgaga 17400 tggcgtgcgc tgacattcgc attcgcgagg tcggcattgg cgcaaaccag cagcgctgca 17460 cgatccgtgc cgccaagctc gagaccggca acccgcagcg ccagatcgcc aaggacgacg 17520 tgctgctcgt ttctggcggc gctcgcggca tcacgcctct ttgcatccgg gagatcacgc 17580 gccagatcgc gggcggcaag tacattctgc ttggccgcag caaggtctct gcgagcgaac 17640 cggcatggtg cgctggcatc actgacgaga aggctgtgca aaaggctgct acccaggagc 17700 tcaagcgcgc ctttagcgct ggcgagggcc ccaagcccac gccccgcgct gtcactaagc 17760 ttgtgggctc tgttcttggc gctcgcgagg tgcgcagctc tattgctgcg attgaagcgc 17820 tcggcggcaa ggccatctac tcgtcgtgcg acgtgaactc tgccgccgac gtggccaagg 17880 ccgtgcgcga tgccgagtcc cagctcggtg cccgcgtctc gggcatcgtt catgcctcgg 17940 gcgtgctccg cgaccgtctc atcgagaaga agctccccga cgagttcgac gccgtctttg 18000 gcaccaaggt caccggtctc gagaacctcc tcgccgccgt cgaccgcgcc aacctcaagc 18060 acatggtcct cttcagctcg ctcgccggct tccacggcaa cgtcggccag tctgactacg 18120 ccatggccaa cgaggccctt aacaagatgg gcctcgagct cgccaaggac gtctcggtca 18180 agtcgatctg cttcggtccc tgggacggtg gcatggtgac gccgcagctc aagaagcagt 18240 tccaggagat gggcgtgcag atcatccccc gcgagggcgg cgctgatacc gtggcgcgca 18300 tcgtgctcgg ctcctcgccg gctgagatcc ttgtcggcaa ctggcgcacc ccgtccaaga 18360 aggtcggctc ggacaccatc accctgcacc gcaagatttc cgccaagtcc aaccccttcc 18420 tcgaggacca cgtcatccag ggccgccgcg tgctgcccat gacgctggcc attggctcgc 18480 tcgcggagac ctgcctcggc ctcttccccg gctactcgct ctgggccatt gacgacgccc 18540 agctcttcaa gggtgtcact gtcgacggcg acgtcaactg cgaggtgacc ctcaccccgt 18600 cgacggcgcc ctcgggccgc gtcaacgtcc aggccacgct caagaccttt tccagcggca 18660 agctggtccc ggcctaccgc gccgtcatcg tgctctccaa ccagggcgcg cccccggcca 18720 acgccaccat gcagccgccc tcgctcgatg ccgatccggc gctccagggc tccgtctacg 18780 acggcaagac cctcttccac ggcccggcct tccgcggcat cgatgacgtg ctctcgtgca 18840 ccaagagcca gcttgtggcc aagtgcagcg ctgtccccgg ctccgacgcc gctcgcggcg 18900 agtttgccac ggacactgac gcccatgacc ccttcgtgaa cgacctggcc tttcaggcca 18960 tgctcgtctg ggtgcgccgc acgctcggcc aggctgcgct ccccaactcg atccagcgca 19020 tcgtccagca ccgcccggtc ccgcaggaca agcccttcta cattaccctc cgctccaacc 19080 agtcgggcgg tcactcccag cacaagcacg cccttcagtt ccacaacgag cagggcgatc 19140 tcttcattga tgtccaggct tcggtcatcg ccacggacag ccttgccttc taaatctaaa 19200 ctaggccatg gtgacttaag gtagttagct taatcactta ggtcacccag tccaagcccg 19260 gcctagcacg atgatcatga gtgttgctat tgatgtatgt taatactata cacagtcatc 19320 gtgtgtgttt tataaaactg taagcttagg atgtttgagg ctaatgtaat tagcactaca 19380 ccgtaataaa tgagaggttt actttatgtt ttaataatgt tcttaccctt gctgctctct 19440 aaactgtaat ggcgtacggt tagctaaact aaagtaaaaa tcgattcgct tgagtaaaat 19500 tagtgaccgg gaaagatcat cgactactac tgctcagaga tgtaatcggt aattaagact 19560 tcaagctcaa taattaatta ggagtaaatc tggcttcgaa tttttaaatt gtcaacttcc 19620 aaagaaggat tggcaagact caatctcaat tgcccaacaa acatatggct ctttacaaat 19680 ttggaatctc tttttgatgg caagaacgtc gtaattcctc tgtcgatctc atgtaattac 19740 caaactcatc tacttattac tttctttata tgcacttact gatgcggccg cttaattaag 19800 atgagtgata ctcaggactc aggactcact ctgctgatca ctagtgctag cctcgaggtc 19860 gacacaactt tgtatacaaa agttgtctag acctagctag gctcagcaac tcatccagtc 19920 aagttcgagt cagtgagttg ggcggccgca gcttatagta aagatttttt tgttaataaa 19980 atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt taataaattt 20040 atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt atggatataa 20100 tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt aaaagatatt 20160 ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg cacaccgctt 20220 ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat gctgccacgt 20280 cagctttcac actatgacac cactggccat acacgttgcc acctcagctt tctcctcttc 20340 tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc ccgcctcttc 20400 acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc tataaataga 20460 gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa atagcaaaac 20520 ggatccacaa ccaaaaatgg ccgctcgcaa cgtgtctgca gcgcatgaga tgcacgatga 20580 aaagcgcatc gccgtcgtcg gcatggccgt ccagtacgcc ggatgcaaaa ccaaggacga 20640 gttctgggag gtgctcatga acggcaaggt cgagtccaag gtgatcagcg acaaacgact 20700 cggctccaac taccgcgccg agcactacaa agcagagcgc agcaagtatg ccgacacctt 20760 ttgcaacgaa acgtacggca cccttgacga gaacgagatc gacaacgagc acgaactcct 20820 cctcaacctc gccaagcagg cactcgcaga gacatccgtc aaagactcga cacgctgcgg 20880 catcgtcagc ggctgcctct cgttccccat ggacaacctc cagggtgaac tcctcaacgt 20940 gtaccaaaac catgtcgaga aaaagctcgg ggcccgcgtc ttcaaggacg cctcccattg 21000 gtccgaacgc gagcagtcca acaaacccga ggccggtgac cgccgcatct tcatggaccc 21060 ggcctccttc gtcgccgaag aactcaacct cggcgccctt cactactccg tcgacgcagc 21120 atgcgccacg gcgctctacg tgctccgcct cgcgcaggat catctcgtct ccggcgccgc 21180 cgacgtcatg ctctgcggtg ccacctgcct gccggagccc tttttcatcc tttcgggctt 21240 ttccaccttc caggccatgc ccgtcggcac gggccagaac gtgtccatgc cgctgcacaa 21300 ggacagccag ggcctcaccc cgggtgaggg cggctccatc atggtcctca agcgtctcga 21360 tgatgccatc cgcgacggcg accacatcta cggcaccctt ctcggcgcca atgtcagcaa 21420 ctccggcaca ggtctgcccc tcaagcccct tctccccagc gagaaaaagt gcctcatgga 21480 cacctacacg cgcattaacg tgcacccgca caagattcag tacgtcgagt gccacgccac 21540 cggcacgccc cagggtgatc gtgtggaaat cgacgccgtc aaggcctgct ttgaaggcaa 21600 ggtcccccgt ttcggtacca caaagggcaa ctttggacac accctcgtcg cagccggctt 21660 tgccggtatg tgcaaggtcc tcctctccat gaagcatggc atcatcccgc ccaccccggg 21720 tatcgatgac gagaccaaga tggaccctct cgtcgtctcc ggtgaggcca tcccatggcc 21780 agagaccaac ggcgagccca agcgcgccgg tctctcggcc tttggctttg gtggcaccaa 21840 cgcccatgcc gtctttgagg agcatgaccc ctccaacgcc gcctgcacgg gccacgactc 21900 catttctgcg ctctcggccc gctgcggcgg tgaaagcaac atgcgcatcg ccatcactgg 21960 tatggacgcc acctttggcg ctctcaaggg actcgacgcc ttcgagcgcg ccatttacac 22020 cggcgctcac ggtgccatcc cactcccaga aaagcgctgg cgctttctcg gcaaggacaa 22080 ggactttctt gacctctgcg gcgtcaaggc caccccgcac ggctgctaca ttgaagatgt 22140 tgaggtcgac ttccagcgcc tccgcacgcc catgacccct gaagacatgc tcctccctca 22200 gcagcttctg gccgtcacca ccattgaccg cgccatcctc gactcgggaa tgaaaaaggg 22260 tggcaatgtc gccgtctttg tcggcctcgg caccgacctc gagctctacc gtcaccgtgc 22320 tcgcgtcgct ctcaaggagc gcgtccgccc tgaagcctcc aagaagctca atgacatgat 22380 gcagtacatt aacgactgcg gcacatccac atcgtacacc tcgtacattg gcaacctcgt 22440 cgccacgcgc gtctcgtcgc agtggggctt cacgggcccc tcctttacga tcaccgaggg 22500 caacaactcc gtctaccgct gcgccgagct cggcaagtac ctcctcgaga ccggcgaggt 22560 cgatggcgtc gtcgttgcgg gtgtcgatct ctgcggcagt gccgaaaacc tttacgtcaa 22620 gtctcgccgc ttcaaggtgt ccacctccga taccccgcgc gccagctttg acgccgccgc 22680 cgatggctac tttgtcggcg agggctgcgg tgcctttgtg ctcaagcgtg agactagctg 22740 caccaaggac gaccgtatct acgcttgcat ggatgccatc gtccctggca acgtccctag 22800 cgcctgcttg cgcgaggccc tcgaccaggc gcgcgtcaag ccgggcgata tcgagatgct 22860 cgagctcagc gccgactccg cccgccacct caaggacccg tccgtcctgc ccaaggagct 22920 cactgccgag gaggaaatcg gcggccttca gacgatcctt cgtgacgatg acaagctccc 22980 gcgcaacgtc gcaacgggca gtgtcaaggc caccgtcggt gacaccggtt atgcctctgg 23040 tgctgccagc ctcatcaagg ctgcgctttg catctacaac cgctacctgc ccagcaacgg 23100 cgacgactgg gatgaacccg cccctgaggc gccctgggac agcaccctct ttgcgtgcca 23160 gacctcgcgc gcttggctca agaaccctgg cgagcgtcgc tatgcggccg tctcgggcgt 23220 ctccgagacg cgctcgtgct attccgtgct cctctccgaa gccgagggcc actacgagcg 23280 cgagaaccgc atctcgctcg acgaggaggc gcccaagctc attgtgcttc gcgccgactc 23340 ccacgaggag atccttggtc gcctcgacaa gatccgcgag cgcttcttgc agcccacggg 23400 cgccgccccg cgcgagtccg agctcaaggc gcaggcccgc cgcatcttcc tcgagctcct 23460 cggcgagacc cttgcccagg atgccgcttc ttcaggctcg caaaagcccc tcgctctcag 23520 cctcgtctcc acgccctcca agctccagcg cgaggtcgag ctcgcggcca agggtatccc 23580 gcgctgcctc aagatgcgcc gcgattggag ctcccctgct ggcagccgct acgcgcctga 23640 gccgctcgcc agcgaccgcg tcgccttcat gtacggcgaa ggtcgcagcc cttactacgg 23700 catcacccaa gacattcacc gcatttggcc cgaactccac gaggtcatca acgaaaagac 23760 gaaccgtctc tgggccgaag gcgaccgctg ggtcatgccg cgcgccagct tcaagtcgga 23820 gctcgagagc cagcagcaag agtttgatcg caacatgatt gaaatgttcc gtcttggaat 23880 cctcacctca attgccttca ccaatctggc gcgcgacgtt ctcaacatca cgcccaaggc 23940 cgcctttggc ctcagtcttg gcgagatttc catgattttt gccttttcca agaagaacgg 24000 tctcatctcc gaccagctca ccaaggatct tcgcgagtcc gacgtgtgga acaaggctct 24060 ggccgttgaa tttaatgcgc tgcgcgaggc ctggggcatt ccacagagtg tccccaagga 24120 cgagttctgg caaggctaca ttgtgcgcgg caccaagcag gatatcgagg cggccatcgc 24180 cccggacagc aagtacgtgc gcctcaccat catcaatgat gccaacaccg ccctcattag 24240 cggcaagccc gacgcctgca aggctgcgat cgcgcgtctc ggtggcaaca ttcctgcgct 24300 tcccgtgacc cagggcatgt gcggccactg ccccgaggtg ggaccttata ccaaggatat 24360 cgccaagatc catgccaacc ttgagttccc cgttgtcgac ggccttgacc tctggaccac 24420 aatcaaccag aagcgcctcg tgccacgcgc cacgggcgcc aaggacgaat gggccccttc 24480 ttcctttggc gagtacgccg gccagctcta cgagaagcag gctaacttcc cccaaatcgt 24540 cgagaccatt tacaagcaaa actacgacgt ctttgtcgag gttgggccca acaaccaccg 24600 tagcaccgca gtgcgcacca cgcttggtcc ccagcgcaac caccttgctg gcgccatcga 24660 caagcagaac gaggatgctt ggacgaccat cgtcaagctt gtggcttcgc tcaaggccca 24720 ccttgttcct ggcgtcacga tctcgccgct gtaccactcc aagcttgtgg cggaggctga 24780 ggcttgctac gctgcgctct gcaagggtga aaagcccaag aagaacaagt ttgtgcgcaa 24840 gattcagctc aacggtcgct tcaacagcaa ggcggacccc atctcctcgg ccgatcttgc 24900 cagctttccg cctgcggacc ctgccattga agccgccatc tcgagccgca tcatgaagcc 24960 ggttgctccg aagttctacg cgcgtctcaa cattgacgag caggacgaga cccgtgatcc 25020 gatcctcaac aaggacaacg cgccgtcttc cagctctagc tcctcttcca gctcttccag 25080 ctcttccagc ccgtcgccag ctccgtccgc cccagtgcaa aagaaggctg ctccggccgc 25140 ggagaccaag gctgttgctt cggctgacgc acttcgcagt gccctgctcg atctcgacag 25200 tatgcttgcg ctgagctctg ccagtgcctc cggcaacctt gttgagactg cgcctagcga 25260 cgcctcggtc attgtgccgc cctgcaacat tgcggatctc ggcagccgcg ccttcatgaa 25320 aacgtacggt gtttcggcgc ctctgtacac gggcgccatg gccaagggca ttgcctctgc 25380 ggacctcgtc attgccgccg gccgccaggg catccttgcg tcctttggcg ccggcggact 25440 tcccatgcag gttgtgcgtg agtccatcga aaagattcag gccgccctgc ccaatggccc 25500 gtacgctgtc aaccttatcc attctccctt tgacagcaac ctcgaaaagg gcaatgtcga 25560 tctcttcctc gagaagggtg tcacctttgt cgaggcctcg gcctttatga cgctcacccc 25620 gcaggtcgtg cggtaccgcg cggctggcct cacgcgcaac gccgacggct cggtcaacat 25680 ccgcaaccgt atcattggca aggtctcgcg caccgagctc gccgagatgt tcatgcgtcc 25740 tgcgcccgag caccttcttc agaagctcat tgcttccggc gagatcaacc aggagcaggc 25800 cgagctcgcc cgccgtgttc ccgtcgctga cgacatcgcg gtcgaagctg actcgggtgg 25860 ccacaccgac aaccgcccca tccacgtcat tctgcccctc atcatcaacc ttcgcgaccg 25920 ccttcaccgc gagtgcggct acccggccaa ccttcgcgtc cgtgtgggcg ccggcggtgg 25980 cattgggtgc ccccaggcgg cgctggccac cttcaacatg ggtgcctcct ttattgtcac 26040 cggcaccgtg aaccaggtcg ccaagcagtc gggcacgtgc gacaatgtgc gcaagcagct 26100 cgcgaaggcc acttactcgg acgtatgcat ggccccggct gccgacatgt tcgaggaagg 26160 cgtcaagctt caggtcctca agaagggaac catgtttccc tcgcgcgcca acaagctcta 26220 cgagctcttt tgcaagtacg actcgttcga gtccatgccc cccgcagagc ttgcgcgcgt 26280 cgagaagcgc atcttcagcc gcgcgctcga agaggtctgg gacgagacca aaaactttta 26340 cattaaccgt cttcacaacc cggagaagat ccagcgcgcc gagcgcgacc ccaagctcaa 26400 gatgtcgctg tgctttcgct ggtacctgag cctggcgagc cgctgggcca acactggagc 26460 ttccgatcgc gtcatggact accaggtctg gtgcggtcct gccattggtt ccttcaacga 26520 tttcatcaag ggaacttacc ttgatccggc cgtcgcaaac gagtacccgt gcgtcgttca 26580 gattaacaag cagatccttc gtggagcgtg cttcttgcgc cgtctcgaaa ttctgcgcaa 26640 cgcacgcctt tccgatggcg ctgccgctct tgtggccagc atcgatgaca catacgtccc 26700 ggccgagaag ctgtaagcgg ccgcatttaa atggatccac taggccatgg tgacttaagg 26760 tagttagctt aatcacttag gtcacccagt ccaagcccgg cctagcacga tgatcatgag 26820 tgttgctatt gatgtatgtt aatactatac acagtcatcg tgtgtgtttt ataaaactgt 26880 aagcttagga tgtttgaggc taatgtaatt agcactacac cgtaataaat gagaggttta 26940 ctttatgttt taataatgtt cttacccttg ctgctctcta aactgtaatg gcgtacggtt 27000 agctaaacta aagtaaaaat cgattcgctt gagtaaaatt agtgaccggg aaagatcatc 27060 gactactact gctcagagat gtaatcggta attaagactt caagctcaat aattaattag 27120 gagtaaatct ggcttcgaat ttttaaattg tcaacttcca aagaaggatt ggcaagactc 27180 aatctcaatt gcccaacaaa catatggctc tttacaaatt tggaatctct ttttgatggc 27240 aagaacgtcg taattcctct gtcgatctca tgtaattacc aaactcatct acttattact 27300 ttctttatat gcacttactg atgcggccgc ttaattaatc tgacctgacc actagacctg 27360 agtgagtaca gatgtgctac tagtgctagc ctcgaggtcg accaactttt ctatacaaag 27420 ttgtctagac ctagctagga ctaggcactg agtgactcac tgacctgact gagtgactgg 27480 cggccgcagc ttatagtaaa gatttttttg ttaataaaat gaaagttaat atttatggat 27540 ttatttgaac atatttacaa gtttttgtta ataaatttat ttacagaagt gttaaatttg 27600 aaaatgtgtt aaatgagagt taatatttat ggatataata ttactttatt taaaaataaa 27660 atgaatttat attaaaatat gatttggtaa aagatattat aaagaggaac ttggatgaga 27720 aagacgacga tgggcaaaag ttgcatggca caccgctttt gttcagtaac atctgcactg 27780 tggcgccttt ttcacactca ccatgcatgc tgccacgtca gctttcacac tatgacacca 27840 ctggccatac acgttgccac ctcagctttc tcctcttctc actatgacac gactggccat 27900 gcatgcatgc atgctgccac ctcagctccc gcctcttcac ccgtgtcttt ctctcatgtc 27960 gcagtgctgc gtgccaacct cttctctcta taaatagaga gaagagaatg atggttaaac 28020 tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg atccacaacc aaaacatatg 28080 gcgctccgtg tcaagacgaa caagaagcca tgctgggaga tgaccaagga ggagctgacc 28140 agcggcaaga ccgaggtgtt caactatgag gaactcctcg agttcgcaga gggcgacatc 28200 gccaaggtct tcggacccga gttcgccgtc atcgacaagt acccgcgccg cgtgcgcctg 28260 cccgcccgcg agtacctgct cgtgacccgc gtcaccctca tggacgccga ggtcaacaac 28320 taccgcgtcg gcgcccgcat ggtcaccgag tacgatctcc ccgtcaacgg agagctctcc 28380 gagggcggag actgcccctg ggccgtcctg gtcgagagtg gccagtgcga tctcatgctc 28440 atctcctaca tgggcattga cttccagaac cagggcgacc gcgtctaccg cctgctcaac 28500 accacgctca ccttttacgg cgtggcccac gagggcgaga ccctcgagta cgacattcgc 28560 gtcaccggct tcgccaagcg tctcgacggc ggcatctcca tgttcttctt cgagtacgac 28620 tgctacgtca acggccgcct cctcatcgag atgcgcgatg gctgcgccgg cttcttcacc 28680 aacgaggagc tcgacgccgg caagggcgtc gtcttcaccc gcggcgacct cgccgcccgc 28740 gccaagatcc caaagcagga cgtctccccc tacgccgtcg ccccctgcct ccacaagacc 28800 aagctcaacg aaaaggagat gcagaccctc gtcgacaagg actgggcatc cgtctttggc 28860 tccaagaacg gcatgccgga aatcaactac aaactctgcg cgcgtaagat gctcatgatt 28920 gaccgcgtca ccagcattga ccacaagggc ggtgtctacg gcctcggtca gctcgtcggt 28980 gaaaagatcc tcgagcgcga ccactggtac tttccctgcc actttgtcaa ggatcaggtc 29040 atggccggat ccctcgtctc cgacggctgc agccagatgc tcaagatgta catgatctgg 29100 ctcggcctcc acctcaccac cggacccttt gacttccgcc cggtcaacgg ccaccccaac 29160 aaggtccgct gccgcggcca aatctccccg cacaagggca agctcgtcta cgtcatggag 29220 atcaaggaga tgggcttcga cgaggacaac gacccgtacg ccattgccga cgtcaacatc 29280 attgatgtcg acttcgaaaa gggccaggac tttagcctcg accgcatcag cgactacggc 29340 aagggcgacc tcaacaagaa gatcgtcgtc gactttaagg gcatcgctct caagatgcag 29400 aagcgctcca ccaacaagaa cccctccaag gttcagcccg tctttgccaa cggcgccgcc 29460 actgtcggcc ccgaggcctc caaggcttcc tccggcgcca gcgccagcgc cagcgccgcc 29520 ccggccaagc ctgccttcag cgccgatgtt cttgcgccca agcccgttgc ccttcccgag 29580 cacatcctca agggcgacgc cctcgccccc aaggagatgt cctggcaccc catggcccgc 29640 atcccgggca acccgacgcc ctcttttgcg ccctcggcct acaagccgcg caacatcgcc 29700 tttacgccct tccccggcaa ccccaacgat aacgaccaca ccccgggcaa gatgccgctc 29760 acctggttca acatggccga gttcatggcc ggcaaggtca gcatgtgcct cggccccgag 29820 ttcgccaagt tcgacgactc gaacaccagc cgcagccccg cttgggacct cgctctcgtc 29880 acccgcgccg tgtctgtgtc tgacctcaag cacgtcaact accgcaacat cgacctcgac 29940 ccctccaagg gtaccatggt cggcgagttc gactgccccg cggacgcctg gttctacaag 30000 ggcgcctgca acgatgccca catgccgtac tcgatcctca tggagatcgc cctccagacc 30060 tcgggtgtgc tcacctcggt gctcaaggcg cccctgacca tggagaagga cgacatcctc 30120 ttccgcaacc tcgacgccaa cgccgagttc gtgcgcgccg acctcgacta ccgcggcaag 30180 actatccgca acgtcaccaa gtgcactggc tacagcatgc tcggcgagat gggcgtccac 30240 cgcttcacct ttgagctcta cgtcgatgat gtgctctttt acaagggctc gacctcgttc 30300 ggctggttcg tgcccgaggt ctttgccgcc caggccggcc tcgacaacgg ccgcaagtcg 30360 gagccctggt tcattgagaa caaggttccg gcctcgcagg tctcctcctt tgacgtgcgc 30420 cccaacggca gcggccgcac cgccatcttc gccaacgccc ccagcggcgc ccagctcaac 30480 cgccgcacgg accagggcca gtacctcgac gccgtcgaca ttgtctccgg cagcggcaag 30540 aagagcctcg gctacgccca cggttccaag acggtcaacc cgaacgactg gttcttctcg 30600 tgccactttt ggtttgactc ggtcatgccc ggaagtctcg gtgtcgagtc catgttccag 30660 ctcgtcgagg ccatcgccgc ccacgaggat ctcgctggca agcacggcat tgccaacccc 30720 acctttgtgc acgccccggg caagatcagc tggaagtacc gcggccagct cacgcccaag 30780 agcaagaaga tggactcgga ggtccacatc gtgtccgtgg acgcccacga cggcgttgtc 30840 gacctcgtcg ccgacggctt cctctgggcc gacagcctcc gcgtctactc ggtgagcaac 30900 attcgcgtgc gcatcgcctc cggtgaggcc cctgccgccg cctcctccgc cgcctctgtg 30960 ggctcctcgg cttcgtccgt cgagcgcacg cgctcgagcc ccgctgtcgc ctccggcccg 31020 gcccagacca tcgacctcaa gcagctcaag accgagctcc tcgagctcga tgccccgctc 31080 tacctctcgc aggacccgac cagcggccag ctcaagaagc acaccgacgt ggcctccggc 31140 caggccacca tcgtgcagcc ctgcacgctc ggcgacctcg gtgaccgctc cttcatggag 31200 acctacggcg tcgtcgcccc gctgtacacg ggcgccatgg ccaagggcat tgcctcggcg 31260 gacctcgtca tcgccgccgg caagcgcaag atcctcggct cctttggcgc cggcggcctc 31320 cccatgcacc acgtgcgcgc cgccctcgag aagatccagg ccgccctgcc tcagggcccc 31380 tacgccgtca acctcatcca ctcgcctttt gacagcaacc tcgagaaggg caacgtcgat 31440 ctcttcctcg agaagggcgt cactgtggtg gaggcctcgg cattcatgac cctcaccccg 31500 caggtcgtgc gctaccgcgc cgccggcctc tcgcgcaacg ccgacggttc ggtcaacatc 31560 cgcaaccgca tcatcggcaa ggtctcgcgc accgagctcg ccgagatgtt catccgcccg 31620 gccccggagc acctcctcga gaagctcatc gcctcgggcg agatcaccca ggagcaggcc 31680 gagctcgcgc gccgcgttcc cgtcgccgac gatatcgctg tcgaggctga ctcgggcggc 31740 cacaccgaca accgccccat ccacgtcatc ctcccgctca tcatcaacct ccgcaaccgc 31800 ctgcaccgcg agtgcggcta ccccgcgcac ctccgcgtcc gcgttggcgc cggcggtggc 31860 gtcggctgcc cgcaggccgc cgccgccgcg ctcaccatgg gcgccgcctt catcgtcacc 31920 ggcactgtca accaggtcgc caagcagtcc ggcacctgcg acaacgtgcg caagcagctc 31980 tcgcaggcca cctactcgga tatctgcatg gccccggccg ccgacatgtt cgaggagggc 32040 gtcaagctcc aggtcctcaa gaagggaacc atgttcccct cgcgcgccaa caagctctac 32100 gagctctttt gcaagtacga ctccttcgac tccatgcctc ctgccgagct cgagcgcatc 32160 gagaagcgta tcttcaagcg cgcactccag gaggtctggg aggagaccaa ggacttttac 32220 attaacggtc tcaagaaccc ggagaagatc cagcgcgccg agcacgaccc caagctcaag 32280 atgtcgctct gcttccgctg gtaccttggt cttgccagcc gctgggccaa catgggcgcc 32340 ccggaccgcg tcatggacta ccaggtctgg tgtggcccgg ccattggcgc cttcaacgac 32400 ttcatcaagg gcacctacct cgaccccgct gtctccaacg agtacccctg tgtcgtccag 32460 atcaacctgc aaatcctccg tggtgcctgc tacctgcgcc gtctcaacgc cctgcgcaac 32520 gacccgcgca ttgacctcga gaccgaggat gctgcctttg tctacgagcc caccaacgcg 32580 ctctaagcta gcatttaaat ctaggccatg gtgacttaag gtagttagct taatcactta 32640 ggtcacccag tccaagcccg gcctagcacg atgatcatga gtgttgctat tgatgtatgt 32700 taatactata cacagtcatc gtgtgtgttt tataaaactg taagcttagg atgtttgagg 32760 ctaatgtaat tagcactaca ccgtaataaa tgagaggttt actttatgtt ttaataatgt 32820 tcttaccctt gctgctctct aaactgtaat ggcgtacggt tagctaaact aaagtaaaaa 32880 tcgattcgct tgagtaaaat tagtgaccgg gaaagatcat cgactactac tgctcagaga 32940 tgtaatcggt aattaagact tcaagctcaa taattaatta ggagtaaatc tggcttcgaa 33000 tttttaaatt gtcaacttcc aaagaaggat tggcaagact caatctcaat tgcccaacaa 33060 acatatatgg ctctttacaa atttggaatc tctttttgat ggcaagaacg tcgtaattcc 33120 tctgtcgatc tcatgtaatt accaaactca tctacttatt actttcttta tatgcactta 33180 ctgatgcggc cgcttaatta atcacgagtg gatgactgtc acgactgact catgactgac 33240 tactagtgct agcctcgagg tcgacacaac tttgtataat aaagttgtct agacctagga 33300 gctgtgctgg gtaactcaca tgagtcactg gtcacatcag cggccgcagc ttatagtaaa 33360 gatttttttg ttaataaaat gaaagttaat atttatggat ttatttgaac atatttacaa 33420 gtttttgtta ataaatttat ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt 33480 taatatttat ggatataata ttactttatt taaaaataaa atgaatttat attaaaatat 33540 gatttggtaa aagatattat aaagaggaac ttggatgaga aagacgacga tgggcaaaag 33600 ttgcatggca caccgctttt gttcagtaac atctgcactg tggcgccttt ttcacactca 33660 ccatgcatgc tgccacgtca gctttcacac tatgacacca ctggccatac acgttgccac 33720 ctcagctttc tcctcttctc actatgacac gactggccat gcatgcatgc atgctgccac 33780 ctcagctccc gcctcttcac ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct 33840 cttctctcta taaatagaga gaagagaatg atggttaaac tcaaaccaaa acaagaaaac 33900 atacacaaat agcaaaacgg atccacaacc aaaaatgttg cagcatactt ggctaccaaa 33960 acccccaaat ttaaccttat tgtcagatga agttcatctc tggcgcattc cccttgacca 34020 accagaatca cagctacagg atttagccgc taccttatct agtgacgaat tagcccgtgc 34080 aaacagattt tattttcccg aacatcgccg gcgttttact gctggtcgtg gtattctccg 34140 cagtatcttg gggggctatt tgggtgtgga accagggcaa gttaaatttg attatgaatc 34200 ccgtggtaaa ccaatattag gcgatcgctt tgccgagagt ggtttattat ttaacttgtc 34260 acactcccag aacttggcct tgtgtgcagt caattacacg cgccaaatcg gcatcgattt 34320 agaatatctc cgccccacat ctgatttaga atcccttgcc aaaaggttct ttttaccgcg 34380 agaatatgaa ttattgcgat cgctacccga tgagcaaaaa caaaaaattt tctttcgtta 34440 ctggacttgt aaagaggctt atcttaaagc aacgggtgac ggcatcgcta aattagagga 34500 aattgaaata gcactaactc ccacagaacc agctaagtta cagacagctc cagcgtggag 34560 tctcctagag ctagtgccag atgataattg tgttgctgct gttgccgtgg cgggttttgg 34620 ctggcagcca aaattctggc attattgagt agttagctta atcacttagg tcacccagtc 34680 caagcccggc ctagcacgat gatcatgagt gttgctattg atgtatgtta atactataca 34740 cagtcatcgt gtgtgtttta taaaactgta agcttaggat gtttgaggct aatgtaatta 34800 gcactacacc gtaataaatg agaggtttac tttatgtttt aataatgttc ttacccttgc 34860 tgctctctaa actgtaatgg cgtacggtta gctaaactaa agtaaaaatc gattcgcttg 34920 agtaaaatta gtgaccggga aagatcatcg actactactg ctcagagatg taatcggtaa 34980 ttaagacttc aagctcaata attaattagg agtaaatctg gcttcgaatt tttaaattgt 35040 caacttccaa agaaggattg gcaagactca atctcaattg cccaacaaac atatggctct 35100 ttacaaattt ggaatctctt tttgatggca agaacgtcgt aattcctctg tcgatctcat 35160 gtaattacca aactcatcta cttattactt tctttatatg cacttactga tgcggccgct 35220 taattaacta ctgtcactga ggccgtagac gagtacggac tgatctaact agtgctagcc 35280 tcgaggtcga cacccagctt tc 35302 <210> 41 <211> 35154 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7369 <400> 41 ttgtacaaag tggttgcggc cgcttaatta aatttaaatt caattaatgc aatcttgatt 60 ttcaacaacg aaggtaatgg cgtaaaagaa aaaatgtatg ttattgtatt gatctttcat 120 gatgttgaag cgtgccataa tatgatgatg tataattaaa atattaactg tcgcatttta 180 ttgaaatggc actgttattt caaccatatc tttgattctg ttacatgaca cgactgcaag 240 aagtaaataa tagacgccgt tgttaaagaa ttgctatcat atgtgcctaa ctagagggaa 300 tttgagcgtc agacctaatc aaatattaca aaatatctca ctctgtcgcc agcaatggtg 360 taatcagcgc agacaaatgg cgtaaagatc gcggaaaaac ctccccgagt ggcatgatag 420 ctgcctctgt attgctgatt tagtcagcct tatttgactt aagggtgccc tcgttagtga 480 caaattgctt tcaaggagac agccatgccc cacactttgt tgaaaaacaa attgcctttg 540 gggagacggt aaagccagtt gctcttcaat aaggaatgtc gaggaggcaa tgtaaccgcc 600 tctggtagta cacttctcta atccaaaaat caatttgtat tcaagatacc gcaaaaaact 660 tatggtttaa accctgcagg actagtccag aaggtaatta tccaagatgt agcatcaaga 720 atccaatgtt tacgggaaaa actatggaag tattatgtaa gctcagcaag aagcagatca 780 atatgcggca catatgcaac ctatgttcaa aaatgaagaa tgtacagata caagatccta 840 tactgccaga atacgaagaa gaatacgtag aaattgaaaa agaagaacca ggcgaagaaa 900 agaatcttga agacgtaagc actgacgaca acaatgaaaa gaagaagata aggtcggtga 960 ttgtgaaaga gacatagagg acacatgtaa ggtggaaaat gtaagggcgg aaagtaacct 1020 tatcacaaag gaatcttatc ccccactact tatcctttta tatttttccg tgtcattttt 1080 gcccttgagt tttcctatat aaggaaccaa gttcggcatt tgtgaaaaca agaaaaaatt 1140 tggtgtaagc tattttcttt gaagtactga ggatacaact tcagagaaat ttgtaagttt 1200 gtaggtacca gatctggatc ccaaaccatg tctccggaga ggagaccagt tgagattagg 1260 ccagctacag cagctgatat ggccgcggtt tgtgatatcg ttaaccatta cattgagacg 1320 tctacagtga actttaggac agagccacaa acaccacaag agtggattga tgatctagag 1380 aggttgcaag atagataccc ttggttggtt gctgaggttg agggtgttgt ggctggtatt 1440 gcttacgctg ggccctggaa ggctaggaac gcttacgatt ggacagttga gagtactgtt 1500 tacgtgtcac ataggcatca aaggttgggc ctaggatcta cattgtacac acatttgctt 1560 aagtctatgg aggcgcaagg ttttaagtct gtggttgctg ttataggcct tccaaacgat 1620 ccatctgtta ggttgcatga ggctttggga tacacagccc ggggtacatt gcgcgcagct 1680 ggatacaagc atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca 1740 gctcctccaa ggccagttag gccagttacc caaatctgag tagttagctt aatcacctag 1800 agctcgatcg gcggcaatag cttcttagcg ccatcccggg ttgatcctat ctgtgttgaa 1860 atagttgcgg tgggcaaggc tctctttcag aaagacaggc ggccaaagga acccaaggtg 1920 aggtgggcta tggctctcag ttccttgtgg aagcgcttgg tctaaggtgc agaggtgtta 1980 gcgggatgaa gcaaaagtgt ccgattgtaa caagatatgt tgatcctacg taaggatatt 2040 aaagtatgta ttcatcacta atataatcag tgtattccaa tatgtactac gatttccaat 2100 gtctttattg tcgccgtatg taatcggcgt cacaaaataa tccccggtga ctttctttta 2160 atccaggatg aaataatatg ttattataat ttttgcgatt tggtccgtta taggaattga 2220 agtgtgcttg aggtcggtcg ccaccactcc catttcataa ttttacatgt atttgaaaaa 2280 taaaaattta tggtattcaa tttaaacacg tatacttgta aagaatgata tcttgaaaga 2340 aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc caagcaaaaa 2400 cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat tatatcagct 2460 ggtacattgc cgtagatgaa agactgagtg cgatattatg gtgtaataca taggaattcg 2520 tttaaacgat ctgcgtctaa ttttcggtcc aacttgcaca ggaaagacgt cgaccgcggt 2580 agctcttgcc cagcagactg ggcttccagt cctttcgctc gatcgggtcc aatgttgtcc 2640 tcagctgtga accggaagcg gacgaccaac agtggaagaa ctgaaaggaa cgagccgtct 2700 ataccttgat gatcggcctc tggtgaaggg tatcatcgca gccaagcaag ctcatgaaag 2760 gctgatgggg gaggtgtata attatgaggc ccacggcggg cttattcttt agggaggatc 2820 tatctcgttg ctcaagtgca tggcgcaaag cagttattgg agtgcggatt ttcgttggca 2880 tattattcgc cacgagttag cagacgaaga gaccttcatg aacgtggcca aggccagagt 2940 taagcagatg ttacgccctg ctgcaggcct ttctattatc caatagttgg ttgatctttg 3000 gaaagagcct cggctgaggc ccatactgaa agagatcgat ggatatcgat atgccatgtt 3060 gtttgctagc cagaaccaga tcacatccga tatgctattg cagcttgacg cagatatgga 3120 ggataagttg attcatggga tcgctcagga gtagctcatc catgcacgcc gacaagaaca 3180 gaaattccgt cgagttaacg cagccgctta cgacggattc gaaggtcatc cattcggaat 3240 gtattagttt gcaccagctc cgcgtcacac ctgtcttcat ttgaataaga tgttagcaat 3300 tgtttttagc tttgtcttgt tgtggcaggg cggcaagtgc ttcagacatc attctgtttt 3360 caaattttat gctggagaac agcttcttaa ttcctttgga aataatagac tgcgtcttaa 3420 aattcagatg tctggatata gatatgattg taaaataacc tatttaagtg tcatttagaa 3480 cataagtttt atgaatgttc ttccattttc gtcatcgaac gaataagagt aaatacacct 3540 tttttaacat tacaaataag ttcttatacg ttgtttatac accgggaatc atttccatta 3600 ttttcgcgca aaagtcacgg atattcgtga aagcgacata aactgcgaaa tttgcgggga 3660 gtgtcttgag tttgcctcga ggctagcgca tgcacataga cacacacatc atctcattga 3720 tgcttggtaa taattgtcat tagattgttt ttatgcatag atgcactcga aatcagccaa 3780 ttttagacaa gtatcaaacg gatgtgactt cagtacatta aaaacgtccg caatgtgtta 3840 ttaagttgtc taagcgtcaa tttgatttac aattgaatat atcctgcccc agccagccaa 3900 cagctcgatt tacaattgaa tatatcctgc cggccggccc acgcgtgtcg aggaattctg 3960 atctggcccc catttggacg tgaatgtaga cacgtcgaaa taaagatttc cgaattagaa 4020 taatttgttt attgctttcg cctataaata cgacggatcg taatttgtcg ttttatcaaa 4080 atgtactttc attttataat aacgctgcgg acatctacat ttttgaattg aaaaaaaatt 4140 ggtaattact ctttcttttt ctccatattg accatcatac tcattgctga tccatgtaga 4200 tttcccggac atgaagccat ttacaattga atatatcctg ccgccgctgc cgctttgcac 4260 ccggtggagc ttgcatgttg gtttctacgc agaactgagc cggttaggca gataatttcc 4320 attgagaact gagccatgtg caccttcccc ccaacacggt gagcgacggg gcaacggagt 4380 gatccacatg ggacttttaa acatcatccg tcggatggcg ttgcgagaga agcagtcgat 4440 ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg atcgcaaagt atttgaacgc 4500 aggtacaatc gagccgacgt tcacgcggaa cgaccaagca agcttggctg ccatttttgg 4560 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc 4620 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca 4680 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga 4740 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg 4800 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa 4860 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca 4920 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt 4980 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct 5040 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc 5100 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg 5160 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 5220 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat 5280 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 5340 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 5400 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 5460 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 5520 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc 5580 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg 5640 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg 5700 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc 5760 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg 5820 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca 5880 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg 5940 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg 6000 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg 6060 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc 6120 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga 6180 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg 6240 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa 6300 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc 6360 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt 6420 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg 6480 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag 6540 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc 6600 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc 6660 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc 6720 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac 6780 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc 6840 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac 6900 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac 6960 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc 7020 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc 7080 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg 7140 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc 7200 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc 7260 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg 7320 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct 7380 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc 7440 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattg 7500 tgattaaggc tcagattcga cggcttggag cggccgacgt gcaggatttc cgcgagatcc 7560 gattgtcggc cctgaagaaa gctccagaga tgttcgggtc cgtttacgag cacgaggaga 7620 aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca 7680 tcgacggcga gatcattggg ctgtcggtct tcaaacagga ggacggcccc aaggacgctc 7740 acaaggcgca tctgtccggc gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg 7800 gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt gatgatcgtc cgacagattc 7860 caacgggaat ctggtggatg cgcatcttca tcctcggcgc acttaatatt tcgctattct 7920 ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg 7980 ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct gctaggtagc ccgatacgat 8040 tgatggcggt cctgggggct atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac 8100 caaacgcagc gctagatcct gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg 8160 cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt gcctctgctc acctttaccg 8220 cctggcaact ggcggccgga ggacttctgc tcgttccagt agctttagtg tttgatccgc 8280 caatcccgat gcctacagga accaatgttc tcggcctggc gtggctcggc ctgatcggag 8340 cgggtttaac ctacttcctt tggttccggg ggatctcgcg actcgaacct acagttgttt 8400 ccttactggg ctttctcagc ccccgagcgc ttagtgggaa tttgtacccc ttatcgaacc 8460 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt 8520 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 8580 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 8640 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 8700 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 8760 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 8820 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 8880 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 8940 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 9000 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 9060 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 9120 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 9180 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca 9240 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg 9300 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt 9360 cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa 9420 ggtggttcta agcctcgtct tgcgatggca tttcgatcca ttcccattcc gcgctcaaga 9480 tggcttcccc tcggcagttc atcagggcta aatcaatcta gccgacttgt ccggtgaaat 9540 gggctgcact ccaacagaaa caatcaaaca aacatacaca gcgacttatt cacacgagct 9600 caaattacaa cggtatatat cctgccagtc agcatcatca caccaaaagt taggcccgaa 9660 tagtttgaaa ttagaaagct cgcaattgag gtctacaggc caaattcgct cttagccgta 9720 caatattact caccggatcc taaccggttt aattaaggcg cgccatgccc gggcaagcgg 9780 ccgcacaagt ttgtacaaaa aagcaggctt ctagacctag gtggagtcat cacgcagact 9840 atctcagcat gtgcgtagca cgcggccgca gcttatagta aagatttttt tgttaataaa 9900 atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt taataaattt 9960 atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt atggatataa 10020 tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt aaaagatatt 10080 ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg cacaccgctt 10140 ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat gctgccacgt 10200 cagctttcac actatgacac cactggccat acacgttgcc acctcagctt tctcctcttc 10260 tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc ccgcctcttc 10320 acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc tataaataga 10380 gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa atagcaaaac 10440 ggatccacaa ccaaaaatgg cagcgaggct gcaagagcag aaaggaggtg agatggacac 10500 gaggattgcg atcataggca tgagcgccat acttccttgt ggaaccacgg tgagagagtc 10560 atgggagacc ataagggctg gcattgattg cctgtctgat cttcctgagg atcgtgttga 10620 tgtgactgcg tactttgacc cagtcaagac cacaaaggac aaaatctact gcaagagagg 10680 aggtttcatt cctgagtatg actttgatgc gcgtgagttt ggtctcaaca tgttccagat 10740 ggaagattct gatgcgaatc agaccatctc acttctcaag gtcaaagaag cacttcaaga 10800 tgctgggatt gatgcacttg gaaaggagaa gaaaaacata ggatgcgtgc ttggcattgg 10860 aggtggacag aagagttcac atgagttcta cagcagactc aactatgtgg ttgtggagaa 10920 agtcttgaga aagatgggga tgccagagga agatgtcaaa gtggctgtgg agaagtacaa 10980 agcgaacttc ccagaatgga gacttgactc tttccctgga ttccttggga atgtcactgc 11040 tggcagatgc accaacacat tcaacttgga tggcatgaac tgcgttgtgg atgctgcatg 11100 tgcaagctcc ttgatagcag tcaaggttgc gattgatgaa ctgctctatg gtgactgcga 11160 catgatggtc actggtgcaa cctgcacaga caactccatt gggatgtaca tggctttctc 11220 aaagacacct gtgttcagca cggacccaag tgtgagagcg tatgatgaga aaacgaaagg 11280 gatgctcatt ggtgagggat cagccatgtt ggtgctcaaa cgttatgcag atgcagtgag 11340 ggatggtgat gagattcatg ctgtgattcg tggttgtgca agctcaagtg atggcaaagc 11400 agctggcatc tacactccca caatcagtgg tcaagaggaa gccttgagac gtgcatacaa 11460 tcgtgcctgc gttgaccctg ccactgtcac tttggttgag ggtcatggga ctggcactcc 11520 agttggagat aggattgagt tgactgcgtt gaggaacttg tttgacaaag cgtatggtga 11580 agggaacaca gagaaagttg cagttggaag tatcaagtcc agtattggtc atttgaaggc 11640 tgttgctggt cttgctggaa tgatcaaggt catcatggct ctcaaacaca agactttgcc 11700 tggaacgatc aatgtggaca atccacccaa tctctatgac aacactccca tcaatgagtc 11760 atccctctac atcaacacga tgaacagacc ctggttccct ccacctggtg ttccgagacg 11820 tgctggaata tccagctttg gctttggagg tgccaactac catgcagtgt tggaagaggc 11880 tgaaccagag cacacgacag cgtacagact caacaaacgt ccacaaccgg tgttgatgat 11940 ggctgcaact cctgctgcgt tgcagagcct ttgtgaagct cagctgaaag agtttgaagc 12000 tgcgatcaag gagaatgaga cagtcaagaa tactgcctac atcaagtgtg tcaagtttgg 12060 agaacagttc aagttccctg ggagcatacc tgcgaccaat gcgagactgg gattcttggt 12120 caaagatgcg gaggatgcgt gctccacgct tcgtgctata tgtgctcagt ttgccaaaga 12180 tgtgaccaaa gaagcgtgga ggcttccgag agaaggagtt tctttccgtg ccaaaggcat 12240 tgccaccaat ggtgctgttg ctgcactctt ctctggacaa ggtgctcagt acacacacat 12300 gttcagtgaa gttgcgatga actggcctca gttcagacag tccattgctg caatggacgc 12360 tgcacagtcc aaggttgctg gaagtgacaa agactttgaa agagtctctc aagtcctgta 12420 cccaaggaaa ccgtacgaac gtgagccaga acaagatcac aagaaaatct cactcacagc 12480 atacagccag ccgtcaaccc tggcatgtgc tcttggtgcc tttgagattt tcaaggaagc 12540 tggattcact ccagactttg cagctggaca cagcttgggt gagtttgctg cgctctatgc 12600 agctggttgt gttgacagag atgagttgtt tgaacttgtt tgcagaaggg caaggatcat 12660 gggaggcaaa gatgcacctg ccacacccaa aggttgcatg gcagctgtca taggacccaa 12720 cgcagagaac atcaaggttc aagctgccaa cgtctggctt ggaaactcaa actctccgtc 12780 tcagactgtc attactgggt ctgttgaagg gattcaagct gaaagtgcca gattgcagaa 12840 ggaagggttc agagttgtgc ctcttgcttg tgagtctgcc ttccattctc cacagatgga 12900 aaatgccagc tcagctttca aagatgtcat atccaaggtt tccttccgta cacccaaagc 12960 agaaacaaag ctcttcagca atgtttctgg agagacctat ccgacggatg ccagagagat 13020 gctcactcaa cacatgacct catctgtgaa gttcctgacc caagtgagga acatgcacca 13080 agctggtgcg agaatctttg ttgagtttgg accgaaacaa gttctcagca aacttgtttc 13140 tgaaaccctc aaagatgacc cgtctgttgt cacagtgtct gtgaacccag cttctggcac 13200 ggactctgac atacagttga gggatgctgc agttcagctg gttgtggctg gtgtcaatct 13260 tcaagggttt gacaagtggg atgctcctga tgccacgagg atgcaagcca tcaagaaaaa 13320 gagaaccaca ctcagacttt cagctgccac ctatgtcagt gacaagacca agaaagtgag 13380 ggatgctgcc atgaacgacg gaagatgtgt cacatacctc aagggtgctg caccactcat 13440 caaggctcct gaacctgtgg ttgacgaagc tgccaagagg gaggctgaaa ggcttcagaa 13500 ggagcttcaa gacgctcaga ggcagcttga cgacgccaaa cgtgcagctg cagaagccaa 13560 ctccaaactt gctgctgcca aggaggaagc caagactgct gcagcttcag ccaagcctgc 13620 agttgacact gctgttgtgg agaaacatcg tgccatcctc aagtccatgc tggctgagct 13680 ggatggttat ggaagtgttg atgcttcttc ccttcagcaa cagcaacagc aacagactgc 13740 tccggcgcct gttaaagccg ctgcccctgc cgctcccgtt gcaagtgctc ctgctcccgc 13800 cgtgtccaac gagttgcttg agaaagctga gacagttgtg atggaggttc tcgctgccaa 13860 gactggatac gaaacagaca tgattgaggc ggacatggag cttgagactg agttgggaat 13920 tgacagcatc aagagggttg aaattctttc tgaagtccaa gctatgctta acgttgaggc 13980 gaaagatgtt gatgctctct ctaggacgag aacagtgggt gaagttgtca acgctatgaa 14040 ggcagaaatc gctggttctt ccgctccggc gccggcagct gccgcacctg caccggctaa 14100 agcggctcca gctgcagctg caccagctgt ttctaatgag ctgctcgaaa aggcagaaac 14160 tgttgtgatg gaagtccttg ctgccaaaac tggctacgaa actgacatga tagaatcaga 14220 catggagctt gagacggagc ttggaattga ttccatcaag cgtgtggaaa tcctcagtga 14280 agtccaagcc atgctcaatg tggaagctaa ggatgtggat gctctctcaa ggaccagaac 14340 tgtcggtgag gttgtcaatg ccatgaaggc tgagatagct ggaggctccg ctcctgcgcc 14400 ggccgctgcc gctcctggtc ccgctgccgc ggctccagct ccagctgcag ccgctccggc 14460 cgtctcaaac gaactccttg aaaaagctga gaccgtcgtg atggaggtct tggcagctaa 14520 aaccggctat gagactgaca tgattgagtc tgacatggaa ttggagacag aacttgggat 14580 agacagcatc aaacgtgttg aaatcctcag tgaggtccaa gctatgctga acgtggaagc 14640 gaaggatgtt gatgctctgt ccagaacgag gaccgttggt gaggttgtgg atgccatgaa 14700 ggctgaaatt gctggagggt ctgcgcccgc gccagctgca gccgctcctg cacccgcagc 14760 cgcggcacct gctccagctg cacctgcacc tgccgtttct tcagaactct tggagaaggc 14820 tgagacggtt gtgatggagg ttttggctgc aaagactggc tatgagaccg acatgattga 14880 atctgatatg gaattggaga cagagcttgg gatcgattcc atcaaaagag tggagatcct 14940 gagtgaagtc caagccatgc tcaatgttga agctaaggat gttgatgcac tttcaagaac 15000 aaggacagtg ggtgaggttg tggatgccat gaaggcggag attgctggag ggagcgcgcc 15060 tgcgccagct gccgcggcac ctgcaccggc agccgcagct ccagctccag ctgcaccagc 15120 accggccgcg cccgcgccag ctgtttcatc cgaactcctg gaaaaggccg agacggttgt 15180 gatggaggtg ctcgcagcta agactggtta cgagaccgac atgattgaaa gtgacatgga 15240 gcttgaaacc gagttgggaa tagatagcat caaacgtgtt gaaatcttgt ctgaggtcca 15300 agctatgttg aacgtggagg caaaagatgt cgatgcgctt tcaagaacca gaacagtcgg 15360 tgaggtcgtg gacgccatga aggctgaaat tgccggaagc tctgctagtg cgccagctgc 15420 agccgcacct gcacctgctg ccgcggcacc tgctccagct gcagccgctc ctgccgtcag 15480 caatgagttg ctggagaaag ccgaaacagt tgtgatggaa gtccttgctg caaaaactgg 15540 ttacgaaacg gacatgattg agtcagacat ggaattggag accgaacttg gaatagattc 15600 catcaagaga gtggagatcc tctctgaggt gcaagccatg ctcaacgttg aggctaaaga 15660 tgttgatgca ctcagtagga caagaactgt gggggaagtt gtcgatgcga tgaaggctga 15720 gattgctgga ggctctgctc ctgcgccggc agctgccgca cctgcacccg cagccgcggc 15780 accagctgtc agcaacgaat tgcttgagaa ggctgaaaca gttgtcatgg aagtgctggc 15840 tgcaaaaacc ggctatgaga ctgacatgat tgagtctgac atggaacttg agacggaatt 15900 gggcattgac agcatcaagc gtgttgagat cctcagtgaa gtccaagcaa tgctcaatgt 15960 ggaggctaag gatgtggatg ccctctcaag gaccagaaca gttggtgagg tcgttgatgc 16020 gatgaaggcg gagatcgctg gaagttccgc gcctgcgcca gctgcagccg ctcctgcacc 16080 cgcagccgcg gcaccggctc cagctgcagc cgcaccggcc gtctcctcag aactcctgga 16140 aaaggctgag acagttgtga tggaagtcct tgctgcgaaa actggctatg agaccgacat 16200 gattgaatct gacatggaac tggagaccga gcttggaatt gactccatca aacgtgttga 16260 aatcctctct gaggttcaag cgatgttgaa tgtggaggcc aaagatgttg atgctctttc 16320 cagaacaagg acggtgggag aggtggttga tgccatgaag gctgagatag ctgggggttc 16380 tgctcctgcg ccagctgcag ccgctcctgc tcccgctgcc gcggcaccag ctgtcagcaa 16440 cgaactcctt gaaaaagctg aaactgttgt gatggaggtt ttggctgcaa agactggata 16500 cgagactgac atgatagagt cagacatgga gttggaaaca gagcttggga ttgatagcat 16560 caagcgtgtg gaaatccttt ctgaggttca agccatgctg aacgttgagg ccaaagatgt 16620 cgatgctttg tcaaggacca gaacggttgg agaagtggtc gatgccatga aggctgagat 16680 agctggtgga tcagcccctg ctccggctgc agccgctcct gcaagtgccg gtgctgcacc 16740 agctgtgaag attgattcag ttcatggtgc tgactgtgac gatttgtcac tgatgcacgc 16800 aaaggtggtt gacatcagaa ggccagatga actgattctg gaaagaccgg agaaccgtcc 16860 agttcttgtc gttgacgatg gctcagagtt gacacttgct cttgtgaggg tgttgggagc 16920 ctgtgcagtt gtcttgacct ttgagggatt gcagttggct cagagagctg gtgcagctgc 16980 cattcgtcat gttctggcta aggacctttc agcagaatca gctgagaagg ccatcaaaga 17040 ggctgagcaa agatttggag cgttgggagg cttcataagc cagcaagcag aaaggtttga 17100 accagctgag atactgggtt tcacactcat gtgtgcgaag tttgccaaag cctctctctg 17160 cactgctgtg gctggaggtc gtcctgcctt catcggtgtg gctaggcttg acggcagact 17220 tggattcacc tctcaaggca cttctgacgc actcaagaga gcacagcgtg gagcgatctt 17280 tggactctgc aagacaatag ggttggagtg gtcagaatct gatgtgttca gccgtggtgt 17340 ggacattgct caagggatgc acccagaaga tgctgcagtt gccatagtta gggagatggc 17400 gtgtgctgac atcagaatca gagaggttgg gattggagca aaccagcaaa gatgcacaat 17460 cagagctgcc aagttggaga ctgggaaccc tcagagacag atagcgaaag acgatgtgct 17520 tctggtttct ggaggtgctc gtgggatcac acctctttgc atccgtgaga tcacgcgtca 17580 gattgctgga ggcaagtaca ttctgttggg tcgtagcaaa gtttctgcgt ctgaaccagc 17640 atggtgtgct ggcatcactg atgagaaggc tgtgcagaaa gctgccaccc aagagttgaa 17700 gagggccttc agtgctggtg aaggacccaa acccactcca agggctgtca cgaaacttgt 17760 gggatctgtt cttggtgctc gtgaagtgag aagctccatt gctgccattg aagctctggg 17820 tggcaaagcg atctacagct cttgtgatgt gaactctgct gcagatgttg ccaaagcagt 17880 tagggatgca gagtcccagc ttggtgccag agttagtgga atcgttcacg cttctggtgt 17940 gctccgtgat cgtctcatag agaagaaact ccctgacgag tttgatgctg tctttgggac 18000 caaagtcact gggttggaga atcttctggc tgcagttgat cgtgccaacc tcaaacacat 18060 ggttctcttc agctcccttg ctggtttcca cggcaatgtt ggtcagtctg actatgcgat 18120 ggcaaacgaa gctctcaaca agatgggttt ggaacttgcg aaggatgtca gtgtcaagtc 18180 catctgcttt ggtccctggg atggtgggat ggtgacacct caactcaaga aacagtttca 18240 agaaatggga gttcagatca ttcctcgtga aggaggtgct gatacagttg cgaggattgt 18300 gttgggatca agtcctgctg agatccttgt tggaaactgg aggacaccat ccaagaaagt 18360 tggaagtgac acgatcaccc tgcatagaaa gatcagtgcc aagtccaatc ccttcttgga 18420 ggatcatgtc atacaaggca gaagggtgtt gccaatgacc cttgctattg ggagccttgc 18480 tgagacctgc cttggactct tccctggcta ctctctctgg gccatagatg acgcacagct 18540 cttcaaaggt gtcactgttg atggagacgt caactgtgaa gtgaccctca caccgtccac 18600 tgctccgagt gggagggtga atgtccaagc gacgctcaag acgttctcaa gtggaaaact 18660 tgttccagcc tacagagctg tcatagtgtt gtccaaccaa ggtgctccac ctgccaatgc 18720 aaccatgcag cctccgagcc ttgatgcaga tcctgctttg caagggtcag tctatgatgg 18780 caagactctc tttcatggtc ctgcgttcag aggcattgat gacgttctca gctgcaccaa 18840 gagccagctt gttgccaagt gttctgctgt tcctggatca gatgcagcca gaggagagtt 18900 tgcaactgac actgatgcac atgatccctt tgtcaatgat cttgcctttc aagcaatgtt 18960 ggtctgggtg aggagaacgc tgggacaagc tgcactcccg aactccattc agaggatagt 19020 ccaacacaga ccggtcccac aagacaaacc cttctacatc accctcagat ccaatcagag 19080 tggtggacat agccagcaca aacatgcact tcagttccac aacgaacaag gtgatctctt 19140 cattgatgtt caagcctctg tcattgcgac agactcactt gcgttctgag tagttagctt 19200 aatcacttag gtcacccagt ccaagcccgg cctagcacga tgatcatgag tgttgctatt 19260 gatgtatgtt aatactatac acagtcatcg tgtgtgtttt ataaaactgt aagcttagga 19320 tgtttgaggc taatgtaatt agcactacac cgtaataaat gagaggttta ctttatgttt 19380 taataatgtt cttacccttg ctgctctcta aactgtaatg gcgtacggtt agctaaacta 19440 aagtaaaaat cgattcgctt gagtaaaatt agtgaccggg aaagatcatc gactactact 19500 gctcagagat gtaatcggta attaagactt caagctcaat aattaattag gagtaaatct 19560 ggcttcgaat ttttaaattg tcaacttcca aagaaggatt ggcaagactc aatctcaatt 19620 gcccaacaaa catatggctc tttacaaatt tggaatctct ttttgatggc aagaacgtcg 19680 taattcctct gtcgatctca tgtaattacc aaactcatct acttattact ttctttatat 19740 gcacttactg atgcggccgc ttaattaaga tgagtgatac tcaggactca ggactcactc 19800 tgctgatcac tagtgctagc ctcgaggtcg acacaacttt gtatacaaaa gttgtctaga 19860 cctaggctca gcaactcatc cagtcaagtt cgagtcagtg agttgggcgg ccgcagctta 19920 tagtaaagat ttttttgtta ataaaatgaa agttaatatt tatggattta tttgaacata 19980 tttacaagtt tttgttaata aatttattta cagaagtgtt aaatttgaaa atgtgttaaa 20040 tgagagttaa tatttatgga tataatatta ctttatttaa aaataaaatg aatttatatt 20100 aaaatatgat ttggtaaaag atattataaa gaggaacttg gatgagaaag acgacgatgg 20160 gcaaaagttg catggcacac cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc 20220 acactcacca tgcatgctgc cacgtcagct ttcacactat gacaccactg gccatacacg 20280 ttgccacctc agctttctcc tcttctcact atgacacgac tggccatgca tgcatgcatg 20340 ctgccacctc agctcccgcc tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg 20400 ccaacctctt ctctctataa atagagagaa gagaatgatg gttaaactca aaccaaaaca 20460 agaaaacata cacaaatagc aaaacggatc cacaaccaaa aatggctgca aggaacgtga 20520 gtgcggctca tgagatgcat gacgagaaga ggatagctgt tgtgggcatg gctgttcagt 20580 atgctggatg caaaacgaaa gacgagttct gggaagttct catgaatgga aaagttgaga 20640 gcaaagtgat cagcgacaaa cgtcttggtt caaattaccg tgctgaacac tacaaagctg 20700 aaaggagcaa gtatgctgac accttctgca atgagacata tggaactctt gatgagaacg 20760 aaatagacaa tgagcatgaa ctccttttga accttgccaa acaagccttg gctgaaacat 20820 ctgtcaaaga ttcaacaaga tgtggcatag tttctggatg cctcagcttc ccgatggaca 20880 acctccaagg tgaactcttg aatgtgtacc agaaccatgt tgagaagaaa cttggtgcca 20940 gagttttcaa agatgcctct cactggtcag aaagagaaca gtccaacaaa ccagaggctg 21000 gtgacagaag aatcttcatg gaccctgctt cctttgttgc tgaggaactc aatcttggcg 21060 ctctccacta ctctgttgat gctgcctgtg ccactgcgct ctatgtgctc agactggcgc 21120 aagatcactt ggtctctggt gcagctgatg tgatgctttg tggtgcaaca tgcctgccgg 21180 agccgttttt catactctct gggttctcaa ccttccaagc catgcctgtt ggaacgggtc 21240 agaatgtgtc catgccgctg cacaaagatt ctcaaggatt gacccctggt gaaggaggtt 21300 ccatcatggt cctcaaacgt ttggatgacg caatcagaga tggagatcac atctatggaa 21360 cacttttggg agccaatgtc agcaacagtg gcactggtct gccacttaaa ccccttttgc 21420 cgtcagagaa aaagtgcctc atggacacct acacacgtat caatgtgcat ccccacaaga 21480 ttcagtatgt tgaatgccat gcaactggga ctccacaagg tgatcgtgtg gaaattgatg 21540 ctgtgaaggc ttgctttgaa ggcaaagtcc cacgttttgg aaccacaaaa gggaactttg 21600 gacacacatt ggtggcagct gggtttgctg gcatgtgcaa agtgcttctc tccatgaagc 21660 acgggatcat tccacccact ccgggaatag atgacgaaac gaagatggac cctctggttg 21720 tctctggtga ggccattccc tggccagaaa cgaatggaga accgaagcgt gctggtctct 21780 cagcgtttgg atttggtggg accaatgctc atgctgtctt tgaggaacat gacccaagca 21840 atgctgcatg cactggccat gactccattt ctgctctctc tgcacgttgt ggtggtgagt 21900 caaacatgag aatagcaatc actgggatgg atgccacttt tggtgctttg aaaggattgg 21960 atgcttttga aagggccatc tacactggtg ctcatggtgc tataccactc ccagaaaaga 22020 gatggagatt cctggggaaa gacaaggact tccttgatct ttgtggagtc aaagcaacac 22080 cgcatggctg ttacattgaa gatgttgaag ttgatttcca gaggctgagg actccaatga 22140 ctcctgaaga tatgctcttg cctcaacagc ttctggctgt caccacaatt gatagggcca 22200 tattggactc tggcatgaag aaaggtggaa atgtggctgt ctttgtgggg ttggggaccg 22260 atcttgaact ctaccgtcac cgtgcaaggg ttgctctcaa ggagagagtg aggcctgaag 22320 ccagcaagaa actcaatgac atgatgcagt acatcaatga ttgtgggaca agcacttctt 22380 acaccagcta cataggcaac ttggttgcaa caagggtcag ttcacaatgg ggcttcacgg 22440 gaccttcctt cacaatcaca gaaggcaaca attctgtgta cagatgtgct gaacttggca 22500 aatacctctt ggaaacggga gaggttgatg gggttgtggt tgcgggtgtt gatctctgtg 22560 ggagtgctga gaacctttac gtcaagtcaa ggcgtttcaa ggtgtctaca tctgacaccc 22620 cgagagccag ctttgatgca gctgctgatg gttactttgt tggagagggc tgtggtgcat 22680 ttgtgctcaa gcgtgagaca agctgcacca aggatgacag aatctatgct tgcatggatg 22740 ccatagttcc tggaaatgtt ccatctgcat gtttgagaga ggcattggat caagccagag 22800 tcaagcctgg agacatcgag atgttggagc tttctgctga ttcagcgagg catttgaagg 22860 acccgtctgt tctcccaaag gaactcactg ctgaggaaga gattggcgga cttcagacga 22920 ttcttcgtga cgatgacaag ctcccaagga atgttgcaac tggaagtgtc aaggccactg 22980 ttggtgacac tggttatgct tctggtgctg ccagtttgat taaggctgcg ctttgcatct 23040 acaaccgtta cctgcccagc aatggagatg actgggatga acctgcacct gaagcaccct 23100 gggacagcac actctttgcg tgccagacct caagagcttg gttgaagaac cctggtgaga 23160 gacgttatgc ggctgtttcc ggagtcagtg aaaccagaag ttgctacagt gtgctgcttt 23220 ctgaagcaga gggacactac gagagagaga acagaatcag tttggatgag gaagcaccca 23280 agctcattgt gttgagagca gactcccatg aagagattct tggaagattg gacaagataa 23340 gggaaaggtt cttgcagccc acgggtgctg cacccagaga gtctgaattg aaggcgcaag 23400 cccgtaggat ctttcttgaa ctccttggtg agacccttgc ccaagatgca gcttcatctg 23460 gaagtcagaa gccccttgct ctcagcttgg tctccactcc atccaagctc cagagagagg 23520 tggaacttgc ggcaaaaggc attccgagat gtctcaagat gagacgtgat tggagttccc 23580 ctgctggaag cagatatgcg cctgagcctt tggccagtga cagagttgcc ttcatgtatg 23640 gagaaggaag gagcccttac tatggcatca cacaagacat tcataggatt tggcctgaac 23700 ttcatgaggt catcaatgag aaaacaaacc gtctctgggc tgaaggtgac agatgggtca 23760 tgccaagggc cagcttcaag agtgagcttg agtcacaaca gcaagagttt gacagaaaca 23820 tgattgaaat gttccgtctt ggaatcctca cctcaattgc cttcacaaac ctggcgagag 23880 atgttctcaa catcacgccc aaagcagcct ttggactcag tttgggagag atttccatga 23940 tctttgcttt ctccaagaaa aacggtttga taagcgatca gttgacaaag gatttgagag 24000 agtcagatgt ctggaacaaa gctctggctg ttgagttcaa tgcgctcaga gaagcctggg 24060 gcattccaca gtctgtccca aaagatgagt tctggcaagg ttacatagtg aggggaacga 24120 aacaagacat agaagcagcc atagcaccag actcaaagta tgtgaggttg accatcatca 24180 atgatgccaa cactgctctc atcagtggaa agcctgatgc atgcaaagct gcgattgctc 24240 gtcttggtgg caacatccct gcgcttccag tcactcaagg catgtgtggg cattgcccag 24300 aggtgggacc ttacaccaag gacatcgcaa agatccacgc caaccttgag ttccctgtgg 24360 ttgatggtct tgatctctgg actaccataa accagaagag gcttgtgcca agagccacgg 24420 gtgccaaaga tgaatgggct ccttcatcct ttggggagta tgctggacag ctctatgaga 24480 aacaagccaa cttccctcag atagttgaaa ccatctacaa gcagaactat gatgtgtttg 24540 ttgaagttgg gccaaacaat cacagaagca cagcagtgag aaccacgctt ggaccacaga 24600 gaaaccacct tgctggtgcc atagacaagc agaacgagga tgcttggacg acaatagtca 24660 agctcgtggc ttcattgaag gctcaccttg ttcctggagt gaccatcagt ccgctctacc 24720 actccaagct cgttgcagag gctgaggctt gttatgctgc gctctgtaaa ggtgagaaac 24780 ccaagaaaaa caagtttgtg agaaagattc agttgaatgg cagattcaac agcaaggcag 24840 accccatctc ctcagcagac cttgcatcct tcccacctgc ggaccctgcg attgaagctg 24900 ccatctcttc aaggatcatg aagcctgtgg ctccgaaatt ctatgcgcgt ctcaacattg 24960 atgagcaaga tgagaccaga gatccgatct tgaacaagga caatgcgcct tccagctcca 25020 gctcttccag cagttcctct tccagcagtt catctccgtc tcctgctcct tcagctccag 25080 ttcagaagaa agctgcccca gctgcggaga caaaagctgt tgccagtgct gatgcacttc 25140 gtagtgccct gcttgatctt gactccatgc ttgcgctcag ctctgcaagt gcttctggca 25200 accttgttga gactgcgcca tctgatgcca gtgtcattgt ccctccgtgc aacattgctg 25260 atcttggcag cagagctttc atgaaaactt atggtgtttc tgcgcctctg tacaccggtg 25320 caatggccaa gggaattgcc tctgcggact tggtgatcgc agctggaagg caaggaatcc 25380 ttgcgtcatt tggtgctgga ggccttccaa tgcaagttgt gcgtgagtcc attgaaaaga 25440 ttcaagctgc cctgcccaat ggaccatatg ctgtcaacct catccattct ccttttgaca 25500 gcaaccttga gaagggaaat gttgatctct tcttggaaaa gggtgtcact tttgttgagg 25560 cttcagcctt catgacgttg acaccgcaag ttgtcagata cagagcggct gggttgacga 25620 ggaatgcaga tgggtcagtc aacataagga acagaatcat tgggaaagtc tccagaactg 25680 agctggctga aatgttcatg cgtcctgcac ctgagcatct tttgcagaag ctcattgctt 25740 ctggggagat caatcaagaa caagctgagt tggccagacg tgttcctgtg gctgatgaca 25800 ttgcggttga agctgattct ggtggacaca ctgacaaccg tccaatccat gtcattctgc 25860 ctctgattat caacctgagg gataggcttc acagagagtg tggataccca gcaaacctga 25920 gggttcgtgt tggtgctggg ggtggcattg ggtgtcccca agctgcgctg gcaactttca 25980 acatgggtgc ctctttcatt gtcactggaa cagtgaacca agttgccaaa caatctggga 26040 cgtgtgacaa tgtgaggaaa cagcttgcga aagcaactta ctcagatgtt tgcatggcac 26100 cggcagctga catgtttgag gaaggtgtga aacttcaagt tttgaagaaa ggaacaatgt 26160 tcccttcaag agccaacaaa ctctatgagt tgttctgcaa gtatgattct tttgagtcca 26220 tgcctccagc agagcttgcg agggttgaga aaagaatctt cagtcgtgca ttggaagagg 26280 tctgggatga gaccaagaac ttctacatca accgtctcca caacccagag aagatacaga 26340 gggctgagag ggaccccaaa ctgaagatgt cactgtgttt cagatggtat ctgtccctgg 26400 cttcaaggtg ggccaacact ggagcttctg acagagtcat ggactaccaa gtttggtgtg 26460 gtcctgccat tggttcattc aatgatttca tcaaaggaac ttaccttgat ccggcagtgg 26520 caaatgagta cccgtgtgtt gtgcagatca acaaacagat tcttagagga gcgtgcttct 26580 tgaggcgtct tgaaatactg agaaatgcaa ggctttcaga tggtgcagct gcacttgtgg 26640 cgagcattga tgacacatat gtccctgctg agaagctgtg agtagttagc ttaatcactt 26700 aggtcaccca gtccaagccc ggcctagcac gatgatcatg agtgttgcta ttgatgtatg 26760 ttaatactat acacagtcat cgtgtgtgtt ttataaaact gtaagcttag gatgtttgag 26820 gctaatgtaa ttagcactac accgtaataa atgagaggtt tactttatgt tttaataatg 26880 ttcttaccct tgctgctctc taaactgtaa tggcgtacgg ttagctaaac taaagtaaaa 26940 atcgattcgc ttgagtaaaa ttagtgaccg ggaaagatca tcgactacta ctgctcagag 27000 atgtaatcgg taattaagac ttcaagctca ataattaatt aggagtaaat ctggcttcga 27060 atttttaaat tgtcaacttc caaagaagga ttggcaagac tcaatctcaa ttgcccaaca 27120 aacatatggc tctttacaaa tttggaatct ctttttgatg gcaagaacgt cgtaattcct 27180 ctgtcgatct catgtaatta ccaaactcat ctacttatta ctttctttat atgcacttac 27240 tgatgcggcc gcttaattaa tctgacctga ccactagacc tgagtgagta cagatgtgct 27300 actagtgcta gcctcgaggt cgaccaactt ttctatacaa agttgtctag acctaggact 27360 aggcactgag tgactcactg acctgactga gtgactggcg gccgcagctt atagtaaaga 27420 tttttttgtt aataaaatga aagttaatat ttatggattt atttgaacat atttacaagt 27480 ttttgttaat aaatttattt acagaagtgt taaatttgaa aatgtgttaa atgagagtta 27540 atatttatgg atataatatt actttattta aaaataaaat gaatttatat taaaatatga 27600 tttggtaaaa gatattataa agaggaactt ggatgagaaa gacgacgatg ggcaaaagtt 27660 gcatggcaca ccgcttttgt tcagtaacat ctgcactgtg gcgccttttt cacactcacc 27720 atgcatgctg ccacgtcagc tttcacacta tgacaccact ggccatacac gttgccacct 27780 cagctttctc ctcttctcac tatgacacga ctggccatgc atgcatgcat gctgccacct 27840 cagctcccgc ctcttcaccc gtgtctttct ctcatgtcgc agtgctgcgt gccaacctct 27900 tctctctata aatagagaga agagaatgat ggttaaactc aaaccaaaac aagaaaacat 27960 acacaaatag caaaacggat ccacaaccaa aaatggcgct tcgtgtcaag accaacaaga 28020 agccatgttg ggagatgacc aaggaggaac tgacctctgg caaaactgag gtgttcaact 28080 atgaggaact cctggagttt gcagagggtg acatagctaa ggtttttggt ccggagtttg 28140 ctgtcataga caagtacccc agaagggtga ggctgcctgc tcgtgaatac ctgcttgtga 28200 caagggtcac actcatggat gccgaggtca acaactacag agttggtgcc agaatggtca 28260 ctgagtatga tcttccagtg aatggcgaat tgtctgaggg aggtgattgt ccctgggcag 28320 ttctggttga gagtggacag tgcgatttga tgctgatatc atacatggga attgatttcc 28380 agaaccaagg tgacagagtt taccgtctgc tcaacaccac gttgacattc tatggggttg 28440 cccatgaggg agagaccctt gagtatgaca tcagagtgac cggttttgcc aaacgtttgg 28500 atggtggcat ctccatgttc ttctttgagt atgactgcta tgtcaatggg aggcttctca 28560 ttgagatgag agatggctgt gctggcttct tcacaaatga ggagcttgat gcgggaaagg 28620 gtgtggtgtt cacaagaggg gatttggctg ccagagctaa gattccaaag caagatgttt 28680 ctccctatgc tgtcgctccc tgccttcaca aaacaaaact caacgaaaag gagatgcaga 28740 ccttggttga caaagattgg gcttccgttt ttggctcaaa gaatggcatg ccggagatca 28800 actacaaact ctgcgcacgt aagatgctca tgattgatag agtcaccagc attgaccata 28860 agggaggtgt gtacggtttg ggtcagttgg ttggtgagaa aatcttggag agggaccatt 28920 ggtacttccc atgccatttt gttaaggatc aagtcatggc tggatctctt gtcagcgacg 28980 gttgttcaca aatgctcaag atgtacatga tctggctcgg tttgcacctt accaccggac 29040 catttgactt cagacccgtc aacggacacc cgaacaaagt taggtgtagg ggtcagatat 29100 ctcctcataa gggaaagttg gtctacgtca tggagatcaa agagatggga ttcgacgagg 29160 ataacgatcc gtacgccatt gctgacgtca acatcattga cgttgatttc gaaaagggtc 29220 aagatttcag ccttgataga atcagcgact acggaaaggg ggatcttaac aaaaagatcg 29280 tcgtggactt caagggcatt gctctcaaga tgcagaagag atccacgaac aagaacccct 29340 ccaaagttca gcccgttttt gctaacggtg cagccactgt gggaccggaa gcgtccaagg 29400 ccagctctgg tgccagtgct agtgcttctg ctgcccctgc gaagcctgct ttctcagcag 29460 atgttcttgc gccaaagcca gttgcacttc ctgaacatat cctcaagggt gacgctctcg 29520 ctccgaaaga aatgtcctgg catcccatgt caaaacttcc tggcaacccg acgccgttct 29580 tctcccctag ttcttaccct ccgagggcaa tttgcttcat ccctttccct ggcaacccac 29640 ttgacaacaa ttgcaaagct ggagaaatgc ctctgaactg gtacaacatg tcagagttca 29700 tgtgtggaaa agttagcaac tgcttgggac cagagttcgc aagatttgat aagtcaaata 29760 cgagcagaag ccctgctttt gatttggctc ttgtgactag agttgttgaa gtcacaaaca 29820 tggaacatgg caagttcctg aatgttgatt gcaatcccag caaaggcaca atggtggggg 29880 agtttgactg tcctcaagat gcgtggttct ttgatggttc ttgcaatgat gggcacatgc 29940 cttattccat catcatggaa atcggacttc agacatccgg tgttctcacc agtgtgttga 30000 aagcaccact gacgatggac aaagatgaca ttctctttag gaaccttgat gcaagtgctg 30060 aaatggtgag gccagatgtg gatgtgaggg ggaaaaccat aaggaatgtg accaagtgca 30120 ctggatatgc aatgttggga aagatgggga ttcacagatt cacgtttgag ttgtctgttg 30180 atggcgtggt tttctacaaa ggaagcactt cctttggatg gttcactcca gaggtgtttg 30240 ctcagcaagc tggacttgac aatgggaaaa agacggagcc ttggtgcaaa actaacaaca 30300 cgagtgtgag aagagttgaa atcgcatcag ccaaaggcaa agaacagctg actgagaaac 30360 ttccagatgc aactaatgct caagttctca gaaggtcaga acagtgtgag tatttggatt 30420 acctcaacat tgctcctgat tctgggctgc atgggaaagg atatgcgcat ggacacaaag 30480 atgttaaccc acaagactgg ttcttctctt gtcacttttg gtttgatcct gtgatgcctg 30540 gatctttggg aattgagtca atgttccagc tcattgaggc atttgcggtg gaccagaaca 30600 taccgggaga atacaatgtg agcaatccga cctttgccca tgcccctgga aaaacggcgt 30660 ggaagtaccg tggtcaattg acaccaaaga accgtgcgat ggactgtgag gtgcacatag 30720 tttcaatcac agcctctcct gaaaatggag gctacgttga catagtggca gatggagcac 30780 tttgggttga tggtctccgt gtgtatgaag ccaaagaact cagagttcgt gttgtcagtg 30840 caaaacctca agccattccg gatgttcaac aacagccacc cagcgcaaaa gcggaccctg 30900 ggaaaactgg agttgcactc agtccgactc agttgagaga tgtcctgctt gaagtggaca 30960 atccattgta tcttggtgtt gaaaactcca atttggtgca gtttgaatcc aaacctgcaa 31020 cttcttcaag gattgtcagt atcaaaccat gctccataag tgatcttggt gacaagtcct 31080 tcatggaaac gtacaatgtg tcagcacctc tgtacactgg agcaatggcc aagggaattg 31140 cgtctgcgga ccttgtcatt gctgctggga agaggaagat attgggttct tttggagcgg 31200 gtgggcttcc aatgcaccat gtgagggcag ctttggagaa gatacaagcg gctctgcctc 31260 aaggaccata tgctgtgaac ctcattcatt caccttttga cagcaacttg gaaaagggga 31320 atgttgatct cttcctcgaa aagggcgtta ctgtggtgga ggcttctgca ttcatgaccc 31380 tcactcccca agttgttcgt taccgtgcag ccggactctc aaggaacgct gatgggagtg 31440 tcaacatcag aaaccgtatc attggaaaag tgtccagaac agaactcgcc gaaatgttca 31500 tcagaccggc tccggaacac ctccttgaga aactcatagc ttctggtgag ataacacaag 31560 aacaagccga acttgcccgt cgtgttcctg tcgcagacga catcgctgtt gaagctgatt 31620 ctggtggtca cactgacaac cgtcccatcc acgtcatctt gcctttgatc atcaacctta 31680 ggaacagact gcacagagag tgtggttatc ctgcgcacct tagagtcaga gttggagctg 31740 gaggcggtgt tggctgccca caagctgctg cggctgccct cacaatggga gctgccttca 31800 tagtgactgg gactgtcaat caagttgcta aacagtccgg gacatgtgac aacgtgagaa 31860 aacaactcag tcaagccacc tacagtgaca tttgcatggc tccagcagct gatatgtttg 31920 aggaaggtgt gaaactccaa gttctcaaga agggaacaat gttcccatca agagctaaca 31980 aattgtacga actcttttgc aagtacgact ctttcgattc aatgcctcct gctgaacttg 32040 aaaggatcga gaagaggatc ttcaagaggg cacttcaaga ggtctgggaa gagaccaagg 32100 acttttacat caatggtttg aagaatccag agaagataca gagagcggaa catgacccca 32160 aactcaagat gtctttgtgt ttcagatggt atcttggtct tgcatcacgt tgggccaaca 32220 tgggtgctcc tgatcgtgtc atggactatc aagtctggtg tggtccagcc attggagcct 32280 tcaatgactt catcaaagga acatatcttg atcctgctgt ctctaatgag tacccatgtg 32340 ttgtccagat caaccttcag attttgcgtg gtgcatgtta cctgaggcgt cttaatgctc 32400 tgagaaatga tccgaggatt gatcttgaga ctgaagatgc tgcctttgtc tatgagccaa 32460 cgaatgcgct ttgagtagtt agcttaatca cttaggtcac ccagtccaag cccggcctag 32520 cacgatgatc atgagtgttg ctattgatgt atgttaatac tatacacagt catcgtgtgt 32580 gttttataaa actgtaagct taggatgttt gaggctaatg taattagcac tacaccgtaa 32640 taaatgagag gtttacttta tgttttaata atgttcttac ccttgctgct ctctaaactg 32700 taatggcgta cggttagcta aactaaagta aaaatcgatt cgcttgagta aaattagtga 32760 ccgggaaaga tcatcgacta ctactgctca gagatgtaat cggtaattaa gacttcaagc 32820 tcaataatta attaggagta aatctggctt cgaattttta aattgtcaac ttccaaagaa 32880 ggattggcaa gactcaatct caattgccca acaaacatat ggctctttac aaatttggaa 32940 tctctttttg atggcaagaa cgtcgtaatt cctctgtcga tctcatgtaa ttaccaaact 33000 catctactta ttactttctt tatatgcact tactgatgcg gccgcttaat taatcacgag 33060 tggatgactg tcacgactga ctcatgactg actactagtg ctagcctcga ggtcgacaca 33120 actttgtata ataaagttgt ctagacctag gagctgtgct gggtaactca catgagtcac 33180 tggtcacatc agcggccgca gcttatagta aagatttttt tgttaataaa atgaaagtta 33240 atatttatgg atttatttga acatatttac aagtttttgt taataaattt atttacagaa 33300 gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt atggatataa tattacttta 33360 tttaaaaata aaatgaattt atattaaaat atgatttggt aaaagatatt ataaagagga 33420 acttggatga gaaagacgac gatgggcaaa agttgcatgg cacaccgctt ttgttcagta 33480 acatctgcac tgtggcgcct ttttcacact caccatgcat gctgccacgt cagctttcac 33540 actatgacac cactggccat acacgttgcc acctcagctt tctcctcttc tcactatgac 33600 acgactggcc atgcatgcat gcatgctgcc acctcagctc ccgcctcttc acccgtgtct 33660 ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc tataaataga gagaagagaa 33720 tgatggttaa actcaaacca aaacaagaaa acatacacaa atagcaaaac ggatccacaa 33780 ccaaaaatgc ttcagcacac ttggcttccg aagcctccca atctgaccct cttgtcagat 33840 gaggttcatc tctggaggat tcctcttgac cagcctgagt cacaacttca agaccttgct 33900 gccaccctga gcagtgatga attggcgagg gcaaacagat tctactttcc agaacacaga 33960 aggcgtttca ctgctgggag aggcatcctc agatccatct tgggtggata cttgggagtg 34020 gaaccgggtc aagtcaagtt tgattatgag tcccgtggga aaccgatcct tggtgacaga 34080 tttgctgaga gtggactcct gttcaacttg tctcacagcc agaaccttgc cttgtgtgct 34140 gtcaactaca cgcgtcaaat aggcattgat cttgaatatc tgcgtccaac atctgacttg 34200 gagtctcttg caaagaggtt ctttctccca agagaatatg aactcttgag gtcactccct 34260 gatgagcaga aacagaagat tttctttcgt tactggactt gcaaagaggc ttatctcaaa 34320 gcaacgggag atggaatagc caaacttgaa gagatcgaga tagcactcac cccaacagaa 34380 cctgccaagc tccaaacagc tcctgcgtgg tctctgttgg agcttgtgcc agatgacaat 34440 tgtgttgcag ctgtggctgt tgcgggtttt ggttggcagc ccaagttctg gcattactga 34500 gtagttagct taatcactta ggtcacccag tccaagcccg gcctagcacg atgatcatga 34560 gtgttgctat tgatgtatgt taatactata cacagtcatc gtgtgtgttt tataaaactg 34620 taagcttagg atgtttgagg ctaatgtaat tagcactaca ccgtaataaa tgagaggttt 34680 actttatgtt ttaataatgt tcttaccctt gctgctctct aaactgtaat ggcgtacggt 34740 tagctaaact aaagtaaaaa tcgattcgct tgagtaaaat tagtgaccgg gaaagatcat 34800 cgactactac tgctcagaga tgtaatcggt aattaagact tcaagctcaa taattaatta 34860 ggagtaaatc tggcttcgaa tttttaaatt gtcaacttcc aaagaaggat tggcaagact 34920 caatctcaat tgcccaacaa acatatggct ctttacaaat ttggaatctc tttttgatgg 34980 caagaacgtc gtaattcctc tgtcgatctc atgtaattac caaactcatc tacttattac 35040 tttctttata tgcacttact gatgcggccg cttaattaac tactgtcact gaggccgtag 35100 acgagtacgg actgatctaa ctagtgctag cctcgaggtc gacacccagc tttc 35154 <210> 42 <211> 36102 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7370 <400> 42 ttgtacaaag tggttgcggc cgcttaatta aatttaaatt caattaatgc aatcttgatt 60 ttcaacaacg aaggtaatgg cgtaaaagaa aaaatgtatg ttattgtatt gatctttcat 120 gatgttgaag cgtgccataa tatgatgatg tataattaaa atattaactg tcgcatttta 180 ttgaaatggc actgttattt caaccatatc tttgattctg ttacatgaca cgactgcaag 240 aagtaaataa tagacgccgt tgttaaagaa ttgctatcat atgtgcctaa ctagagggaa 300 tttgagcgtc agacctaatc aaatattaca aaatatctca ctctgtcgcc agcaatggtg 360 taatcagcgc agacaaatgg cgtaaagatc gcggaaaaac ctccccgagt ggcatgatag 420 ctgcctctgt attgctgatt tagtcagcct tatttgactt aagggtgccc tcgttagtga 480 caaattgctt tcaaggagac agccatgccc cacactttgt tgaaaaacaa attgcctttg 540 gggagacggt aaagccagtt gctcttcaat aaggaatgtc gaggaggcaa tgtaaccgcc 600 tctggtagta cacttctcta atccaaaaat caatttgtat tcaagatacc gcaaaaaact 660 tatggtttaa accctgcagg actagtccag aaggtaatta tccaagatgt agcatcaaga 720 atccaatgtt tacgggaaaa actatggaag tattatgtaa gctcagcaag aagcagatca 780 atatgcggca catatgcaac ctatgttcaa aaatgaagaa tgtacagata caagatccta 840 tactgccaga atacgaagaa gaatacgtag aaattgaaaa agaagaacca ggcgaagaaa 900 agaatcttga agacgtaagc actgacgaca acaatgaaaa gaagaagata aggtcggtga 960 ttgtgaaaga gacatagagg acacatgtaa ggtggaaaat gtaagggcgg aaagtaacct 1020 tatcacaaag gaatcttatc ccccactact tatcctttta tatttttccg tgtcattttt 1080 gcccttgagt tttcctatat aaggaaccaa gttcggcatt tgtgaaaaca agaaaaaatt 1140 tggtgtaagc tattttcttt gaagtactga ggatacaact tcagagaaat ttgtaagttt 1200 gtaggtacca gatctggatc ccaaaccatg tctccggaga ggagaccagt tgagattagg 1260 ccagctacag cagctgatat ggccgcggtt tgtgatatcg ttaaccatta cattgagacg 1320 tctacagtga actttaggac agagccacaa acaccacaag agtggattga tgatctagag 1380 aggttgcaag atagataccc ttggttggtt gctgaggttg agggtgttgt ggctggtatt 1440 gcttacgctg ggccctggaa ggctaggaac gcttacgatt ggacagttga gagtactgtt 1500 tacgtgtcac ataggcatca aaggttgggc ctaggatcta cattgtacac acatttgctt 1560 aagtctatgg aggcgcaagg ttttaagtct gtggttgctg ttataggcct tccaaacgat 1620 ccatctgtta ggttgcatga ggctttggga tacacagccc ggggtacatt gcgcgcagct 1680 ggatacaagc atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca 1740 gctcctccaa ggccagttag gccagttacc caaatctgag tagttagctt aatcacctag 1800 agctcgatcg gcggcaatag cttcttagcg ccatcccggg ttgatcctat ctgtgttgaa 1860 atagttgcgg tgggcaaggc tctctttcag aaagacaggc ggccaaagga acccaaggtg 1920 aggtgggcta tggctctcag ttccttgtgg aagcgcttgg tctaaggtgc agaggtgtta 1980 gcgggatgaa gcaaaagtgt ccgattgtaa caagatatgt tgatcctacg taaggatatt 2040 aaagtatgta ttcatcacta atataatcag tgtattccaa tatgtactac gatttccaat 2100 gtctttattg tcgccgtatg taatcggcgt cacaaaataa tccccggtga ctttctttta 2160 atccaggatg aaataatatg ttattataat ttttgcgatt tggtccgtta taggaattga 2220 agtgtgcttg aggtcggtcg ccaccactcc catttcataa ttttacatgt atttgaaaaa 2280 taaaaattta tggtattcaa tttaaacacg tatacttgta aagaatgata tcttgaaaga 2340 aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc caagcaaaaa 2400 cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat tatatcagct 2460 ggtacattgc cgtagatgaa agactgagtg cgatattatg gtgtaataca taggaattcg 2520 tttaaacgat ctgcgtctaa ttttcggtcc aacttgcaca ggaaagacgt cgaccgcggt 2580 agctcttgcc cagcagactg ggcttccagt cctttcgctc gatcgggtcc aatgttgtcc 2640 tcagctgtga accggaagcg gacgaccaac agtggaagaa ctgaaaggaa cgagccgtct 2700 ataccttgat gatcggcctc tggtgaaggg tatcatcgca gccaagcaag ctcatgaaag 2760 gctgatgggg gaggtgtata attatgaggc ccacggcggg cttattcttt agggaggatc 2820 tatctcgttg ctcaagtgca tggcgcaaag cagttattgg agtgcggatt ttcgttggca 2880 tattattcgc cacgagttag cagacgaaga gaccttcatg aacgtggcca aggccagagt 2940 taagcagatg ttacgccctg ctgcaggcct ttctattatc caatagttgg ttgatctttg 3000 gaaagagcct cggctgaggc ccatactgaa agagatcgat ggatatcgat atgccatgtt 3060 gtttgctagc cagaaccaga tcacatccga tatgctattg cagcttgacg cagatatgga 3120 ggataagttg attcatggga tcgctcagga gtagctcatc catgcacgcc gacaagaaca 3180 gaaattccgt cgagttaacg cagccgctta cgacggattc gaaggtcatc cattcggaat 3240 gtattagttt gcaccagctc cgcgtcacac ctgtcttcat ttgaataaga tgttagcaat 3300 tgtttttagc tttgtcttgt tgtggcaggg cggcaagtgc ttcagacatc attctgtttt 3360 caaattttat gctggagaac agcttcttaa ttcctttgga aataatagac tgcgtcttaa 3420 aattcagatg tctggatata gatatgattg taaaataacc tatttaagtg tcatttagaa 3480 cataagtttt atgaatgttc ttccattttc gtcatcgaac gaataagagt aaatacacct 3540 tttttaacat tacaaataag ttcttatacg ttgtttatac accgggaatc atttccatta 3600 ttttcgcgca aaagtcacgg atattcgtga aagcgacata aactgcgaaa tttgcgggga 3660 gtgtcttgag tttgcctcga ggctagcgca tgcacataga cacacacatc atctcattga 3720 tgcttggtaa taattgtcat tagattgttt ttatgcatag atgcactcga aatcagccaa 3780 ttttagacaa gtatcaaacg gatgtgactt cagtacatta aaaacgtccg caatgtgtta 3840 ttaagttgtc taagcgtcaa tttgatttac aattgaatat atcctgcccc agccagccaa 3900 cagctcgatt tacaattgaa tatatcctgc cggccggccc acgcgtgtcg aggaattctg 3960 atctggcccc catttggacg tgaatgtaga cacgtcgaaa taaagatttc cgaattagaa 4020 taatttgttt attgctttcg cctataaata cgacggatcg taatttgtcg ttttatcaaa 4080 atgtactttc attttataat aacgctgcgg acatctacat ttttgaattg aaaaaaaatt 4140 ggtaattact ctttcttttt ctccatattg accatcatac tcattgctga tccatgtaga 4200 tttcccggac atgaagccat ttacaattga atatatcctg ccgccgctgc cgctttgcac 4260 ccggtggagc ttgcatgttg gtttctacgc agaactgagc cggttaggca gataatttcc 4320 attgagaact gagccatgtg caccttcccc ccaacacggt gagcgacggg gcaacggagt 4380 gatccacatg ggacttttaa acatcatccg tcggatggcg ttgcgagaga agcagtcgat 4440 ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg atcgcaaagt atttgaacgc 4500 aggtacaatc gagccgacgt tcacgcggaa cgaccaagca agcttggctg ccatttttgg 4560 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc 4620 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca 4680 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga 4740 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg 4800 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa 4860 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca 4920 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt 4980 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct 5040 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc 5100 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg 5160 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 5220 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat 5280 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 5340 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 5400 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 5460 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 5520 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc 5580 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg 5640 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg 5700 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc 5760 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg 5820 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca 5880 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg 5940 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg 6000 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg 6060 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc 6120 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga 6180 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg 6240 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa 6300 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc 6360 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt 6420 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg 6480 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag 6540 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc 6600 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc 6660 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc 6720 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac 6780 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc 6840 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac 6900 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac 6960 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc 7020 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc 7080 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg 7140 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc 7200 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc 7260 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg 7320 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct 7380 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc 7440 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattg 7500 tgattaaggc tcagattcga cggcttggag cggccgacgt gcaggatttc cgcgagatcc 7560 gattgtcggc cctgaagaaa gctccagaga tgttcgggtc cgtttacgag cacgaggaga 7620 aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca 7680 tcgacggcga gatcattggg ctgtcggtct tcaaacagga ggacggcccc aaggacgctc 7740 acaaggcgca tctgtccggc gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg 7800 gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt gatgatcgtc cgacagattc 7860 caacgggaat ctggtggatg cgcatcttca tcctcggcgc acttaatatt tcgctattct 7920 ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg 7980 ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct gctaggtagc ccgatacgat 8040 tgatggcggt cctgggggct atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac 8100 caaacgcagc gctagatcct gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg 8160 cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt gcctctgctc acctttaccg 8220 cctggcaact ggcggccgga ggacttctgc tcgttccagt agctttagtg tttgatccgc 8280 caatcccgat gcctacagga accaatgttc tcggcctggc gtggctcggc ctgatcggag 8340 cgggtttaac ctacttcctt tggttccggg ggatctcgcg actcgaacct acagttgttt 8400 ccttactggg ctttctcagc ccccgagcgc ttagtgggaa tttgtacccc ttatcgaacc 8460 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt 8520 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 8580 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 8640 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 8700 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 8760 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 8820 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 8880 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 8940 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 9000 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 9060 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 9120 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 9180 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca 9240 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg 9300 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt 9360 cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa 9420 ggtggttcta agcctcgtct tgcgatggca tttcgatcca ttcccattcc gcgctcaaga 9480 tggcttcccc tcggcagttc atcagggcta aatcaatcta gccgacttgt ccggtgaaat 9540 gggctgcact ccaacagaaa caatcaaaca aacatacaca gcgacttatt cacacgagct 9600 caaattacaa cggtatatat cctgccagtc agcatcatca caccaaaagt taggcccgaa 9660 tagtttgaaa ttagaaagct cgcaattgag gtctacaggc caaattcgct cttagccgta 9720 caatattact caccggatcc taaccggttt aattaaggcg cgccatgccc gggcaagcgg 9780 ccgcacaagt ttgtacaaaa aagcaggctt ctagacctag gtggagtcat cacgcagact 9840 atctcagcat gtgcgtagca cgcggccgca gcttatagta aagatttttt tgttaataaa 9900 atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt taataaattt 9960 atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt atggatataa 10020 tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt aaaagatatt 10080 ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg cacaccgctt 10140 ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat gctgccacgt 10200 cagctttcac actatgacac cactggccat acacgttgcc acctcagctt tctcctcttc 10260 tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc ccgcctcttc 10320 acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc tataaataga 10380 gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa atagcaaaac 10440 ggatccacaa ccaaaaatgg cttcctctat gctctcttcc gctactatgg ttgcctctcc 10500 ggctcaggcc actatggtcg ctcctttcaa cggacttaag tcctccgctg ccttcccagc 10560 cacccgcaag gctaacaacg acattacttc catcacaagc aacggcggaa gagttaactg 10620 catgcaggtg tggcctccga ttggaaagaa gaagtttgag actctctctt accttcctga 10680 ccttaccgat tccgaagcag cgaggctgca agagcagaaa ggaggtgaga tggacacgag 10740 gattgcgatc ataggcatga gcgccatact tccttgtgga accacggtga gagagtcatg 10800 ggagaccata agggctggca ttgattgcct gtctgatctt cctgaggatc gtgttgatgt 10860 gactgcgtac tttgacccag tcaagaccac aaaggacaaa atctactgca agagaggagg 10920 tttcattcct gagtatgact ttgatgcgcg tgagtttggt ctcaacatgt tccagatgga 10980 agattctgat gcgaatcaga ccatctcact tctcaaggtc aaagaagcac ttcaagatgc 11040 tgggattgat gcacttggaa aggagaagaa aaacatagga tgcgtgcttg gcattggagg 11100 tggacagaag agttcacatg agttctacag cagactcaac tatgtggttg tggagaaagt 11160 cttgagaaag atggggatgc cagaggaaga tgtcaaagtg gctgtggaga agtacaaagc 11220 gaacttccca gaatggagac ttgactcttt ccctggattc cttgggaatg tcactgctgg 11280 cagatgcacc aacacattca acttggatgg catgaactgc gttgtggatg ctgcatgtgc 11340 aagctccttg atagcagtca aggttgcgat tgatgaactg ctctatggtg actgcgacat 11400 gatggtcact ggtgcaacct gcacagacaa ctccattggg atgtacatgg ctttctcaaa 11460 gacacctgtg ttcagcacgg acccaagtgt gagagcgtat gatgagaaaa cgaaagggat 11520 gctcattggt gagggatcag ccatgttggt gctcaaacgt tatgcagatg cagtgaggga 11580 tggtgatgag attcatgctg tgattcgtgg ttgtgcaagc tcaagtgatg gcaaagcagc 11640 tggcatctac actcccacaa tcagtggtca agaggaagcc ttgagacgtg catacaatcg 11700 tgcctgcgtt gaccctgcca ctgtcacttt ggttgagggt catgggactg gcactccagt 11760 tggagatagg attgagttga ctgcgttgag gaacttgttt gacaaagcgt atggtgaagg 11820 gaacacagag aaagttgcag ttggaagtat caagtccagt attggtcatt tgaaggctgt 11880 tgctggtctt gctggaatga tcaaggtcat catggctctc aaacacaaga ctttgcctgg 11940 aacgatcaat gtggacaatc cacccaatct ctatgacaac actcccatca atgagtcatc 12000 cctctacatc aacacgatga acagaccctg gttccctcca cctggtgttc cgagacgtgc 12060 tggaatatcc agctttggct ttggaggtgc caactaccat gcagtgttgg aagaggctga 12120 accagagcac acgacagcgt acagactcaa caaacgtcca caaccggtgt tgatgatggc 12180 tgcaactcct gctgcgttgc agagcctttg tgaagctcag ctgaaagagt ttgaagctgc 12240 gatcaaggag aatgagacag tcaagaatac tgcctacatc aagtgtgtca agtttggaga 12300 acagttcaag ttccctggga gcatacctgc gaccaatgcg agactgggat tcttggtcaa 12360 agatgcggag gatgcgtgct ccacgcttcg tgctatatgt gctcagtttg ccaaagatgt 12420 gaccaaagaa gcgtggaggc ttccgagaga aggagtttct ttccgtgcca aaggcattgc 12480 caccaatggt gctgttgctg cactcttctc tggacaaggt gctcagtaca cacacatgtt 12540 cagtgaagtt gcgatgaact ggcctcagtt cagacagtcc attgctgcaa tggacgctgc 12600 acagtccaag gttgctggaa gtgacaaaga ctttgaaaga gtctctcaag tcctgtaccc 12660 aaggaaaccg tacgaacgtg agccagaaca agatcacaag aaaatctcac tcacagcata 12720 cagccagccg tcaaccctgg catgtgctct tggtgccttt gagattttca aggaagctgg 12780 attcactcca gactttgcag ctggacacag cttgggtgag tttgctgcgc tctatgcagc 12840 tggttgtgtt gacagagatg agttgtttga acttgtttgc agaagggcaa ggatcatggg 12900 aggcaaagat gcacctgcca cacccaaagg ttgcatggca gctgtcatag gacccaacgc 12960 agagaacatc aaggttcaag ctgccaacgt ctggcttgga aactcaaact ctccgtctca 13020 gactgtcatt actgggtctg ttgaagggat tcaagctgaa agtgccagat tgcagaagga 13080 agggttcaga gttgtgcctc ttgcttgtga gtctgccttc cattctccac agatggaaaa 13140 tgccagctca gctttcaaag atgtcatatc caaggtttcc ttccgtacac ccaaagcaga 13200 aacaaagctc ttcagcaatg tttctggaga gacctatccg acggatgcca gagagatgct 13260 cactcaacac atgacctcat ctgtgaagtt cctgacccaa gtgaggaaca tgcaccaagc 13320 tggtgcgaga atctttgttg agtttggacc gaaacaagtt ctcagcaaac ttgtttctga 13380 aaccctcaaa gatgacccgt ctgttgtcac agtgtctgtg aacccagctt ctggcacgga 13440 ctctgacata cagttgaggg atgctgcagt tcagctggtt gtggctggtg tcaatcttca 13500 agggtttgac aagtgggatg ctcctgatgc cacgaggatg caagccatca agaaaaagag 13560 aaccacactc agactttcag ctgccaccta tgtcagtgac aagaccaaga aagtgaggga 13620 tgctgccatg aacgacggaa gatgtgtcac atacctcaag ggtgctgcac cactcatcaa 13680 ggctcctgaa cctgtggttg acgaagctgc caagagggag gctgaaaggc ttcagaagga 13740 gcttcaagac gctcagaggc agcttgacga cgccaaacgt gcagctgcag aagccaactc 13800 caaacttgct gctgccaagg aggaagccaa gactgctgca gcttcagcca agcctgcagt 13860 tgacactgct gttgtggaga aacatcgtgc catcctcaag tccatgctgg ctgagctgga 13920 tggttatgga agtgttgatg cttcttccct tcagcaacag caacagcaac agactgctcc 13980 ggcgcctgtt aaagccgctg cccctgccgc tcccgttgca agtgctcctg ctcccgccgt 14040 gtccaacgag ttgcttgaga aagctgagac agttgtgatg gaggttctcg ctgccaagac 14100 tggatacgaa acagacatga ttgaggcgga catggagctt gagactgagt tgggaattga 14160 cagcatcaag agggttgaaa ttctttctga agtccaagct atgcttaacg ttgaggcgaa 14220 agatgttgat gctctctcta ggacgagaac agtgggtgaa gttgtcaacg ctatgaaggc 14280 agaaatcgct ggttcttccg ctccggcgcc ggcagctgcc gcacctgcac cggctaaagc 14340 ggctccagct gcagctgcac cagctgtttc taatgagctg ctcgaaaagg cagaaactgt 14400 tgtgatggaa gtccttgctg ccaaaactgg ctacgaaact gacatgatag aatcagacat 14460 ggagcttgag acggagcttg gaattgattc catcaagcgt gtggaaatcc tcagtgaagt 14520 ccaagccatg ctcaatgtgg aagctaagga tgtggatgct ctctcaagga ccagaactgt 14580 cggtgaggtt gtcaatgcca tgaaggctga gatagctgga ggctccgctc ctgcgccggc 14640 cgctgccgct cctggtcccg ctgccgcggc tccagctcca gctgcagccg ctccggccgt 14700 ctcaaacgaa ctccttgaaa aagctgagac cgtcgtgatg gaggtcttgg cagctaaaac 14760 cggctatgag actgacatga ttgagtctga catggaattg gagacagaac ttgggataga 14820 cagcatcaaa cgtgttgaaa tcctcagtga ggtccaagct atgctgaacg tggaagcgaa 14880 ggatgttgat gctctgtcca gaacgaggac cgttggtgag gttgtggatg ccatgaaggc 14940 tgaaattgct ggagggtctg cgcccgcgcc agctgcagcc gctcctgcac ccgcagccgc 15000 ggcacctgct ccagctgcac ctgcacctgc cgtttcttca gaactcttgg agaaggctga 15060 gacggttgtg atggaggttt tggctgcaaa gactggctat gagaccgaca tgattgaatc 15120 tgatatggaa ttggagacag agcttgggat cgattccatc aaaagagtgg agatcctgag 15180 tgaagtccaa gccatgctca atgttgaagc taaggatgtt gatgcacttt caagaacaag 15240 gacagtgggt gaggttgtgg atgccatgaa ggcggagatt gctggaggga gcgcgcctgc 15300 gccagctgcc gcggcacctg caccggcagc cgcagctcca gctccagctg caccagcacc 15360 ggccgcgccc gcgccagctg tttcatccga actcctggaa aaggccgaga cggttgtgat 15420 ggaggtgctc gcagctaaga ctggttacga gaccgacatg attgaaagtg acatggagct 15480 tgaaaccgag ttgggaatag atagcatcaa acgtgttgaa atcttgtctg aggtccaagc 15540 tatgttgaac gtggaggcaa aagatgtcga tgcgctttca agaaccagaa cagtcggtga 15600 ggtcgtggac gccatgaagg ctgaaattgc cggaagctct gctagtgcgc cagctgcagc 15660 cgcacctgca cctgctgccg cggcacctgc tccagctgca gccgctcctg ccgtcagcaa 15720 tgagttgctg gagaaagccg aaacagttgt gatggaagtc cttgctgcaa aaactggtta 15780 cgaaacggac atgattgagt cagacatgga attggagacc gaacttggaa tagattccat 15840 caagagagtg gagatcctct ctgaggtgca agccatgctc aacgttgagg ctaaagatgt 15900 tgatgcactc agtaggacaa gaactgtggg ggaagttgtc gatgcgatga aggctgagat 15960 tgctggaggc tctgctcctg cgccggcagc tgccgcacct gcacccgcag ccgcggcacc 16020 agctgtcagc aacgaattgc ttgagaaggc tgaaacagtt gtcatggaag tgctggctgc 16080 aaaaaccggc tatgagactg acatgattga gtctgacatg gaacttgaga cggaattggg 16140 cattgacagc atcaagcgtg ttgagatcct cagtgaagtc caagcaatgc tcaatgtgga 16200 ggctaaggat gtggatgccc tctcaaggac cagaacagtt ggtgaggtcg ttgatgcgat 16260 gaaggcggag atcgctggaa gttccgcgcc tgcgccagct gcagccgctc ctgcacccgc 16320 agccgcggca ccggctccag ctgcagccgc accggccgtc tcctcagaac tcctggaaaa 16380 ggctgagaca gttgtgatgg aagtccttgc tgcgaaaact ggctatgaga ccgacatgat 16440 tgaatctgac atggaactgg agaccgagct tggaattgac tccatcaaac gtgttgaaat 16500 cctctctgag gttcaagcga tgttgaatgt ggaggccaaa gatgttgatg ctctttccag 16560 aacaaggacg gtgggagagg tggttgatgc catgaaggct gagatagctg ggggttctgc 16620 tcctgcgcca gctgcagccg ctcctgctcc cgctgccgcg gcaccagctg tcagcaacga 16680 actccttgaa aaagctgaaa ctgttgtgat ggaggttttg gctgcaaaga ctggatacga 16740 gactgacatg atagagtcag acatggagtt ggaaacagag cttgggattg atagcatcaa 16800 gcgtgtggaa atcctttctg aggttcaagc catgctgaac gttgaggcca aagatgtcga 16860 tgctttgtca aggaccagaa cggttggaga agtggtcgat gccatgaagg ctgagatagc 16920 tggtggatca gcccctgctc cggctgcagc cgctcctgca agtgccggtg ctgcaccagc 16980 tgtgaagatt gattcagttc atggtgctga ctgtgacgat ttgtcactga tgcacgcaaa 17040 ggtggttgac atcagaaggc cagatgaact gattctggaa agaccggaga accgtccagt 17100 tcttgtcgtt gacgatggct cagagttgac acttgctctt gtgagggtgt tgggagcctg 17160 tgcagttgtc ttgacctttg agggattgca gttggctcag agagctggtg cagctgccat 17220 tcgtcatgtt ctggctaagg acctttcagc agaatcagct gagaaggcca tcaaagaggc 17280 tgagcaaaga tttggagcgt tgggaggctt cataagccag caagcagaaa ggtttgaacc 17340 agctgagata ctgggtttca cactcatgtg tgcgaagttt gccaaagcct ctctctgcac 17400 tgctgtggct ggaggtcgtc ctgccttcat cggtgtggct aggcttgacg gcagacttgg 17460 attcacctct caaggcactt ctgacgcact caagagagca cagcgtggag cgatctttgg 17520 actctgcaag acaatagggt tggagtggtc agaatctgat gtgttcagcc gtggtgtgga 17580 cattgctcaa gggatgcacc cagaagatgc tgcagttgcc atagttaggg agatggcgtg 17640 tgctgacatc agaatcagag aggttgggat tggagcaaac cagcaaagat gcacaatcag 17700 agctgccaag ttggagactg ggaaccctca gagacagata gcgaaagacg atgtgcttct 17760 ggtttctgga ggtgctcgtg ggatcacacc tctttgcatc cgtgagatca cgcgtcagat 17820 tgctggaggc aagtacattc tgttgggtcg tagcaaagtt tctgcgtctg aaccagcatg 17880 gtgtgctggc atcactgatg agaaggctgt gcagaaagct gccacccaag agttgaagag 17940 ggccttcagt gctggtgaag gacccaaacc cactccaagg gctgtcacga aacttgtggg 18000 atctgttctt ggtgctcgtg aagtgagaag ctccattgct gccattgaag ctctgggtgg 18060 caaagcgatc tacagctctt gtgatgtgaa ctctgctgca gatgttgcca aagcagttag 18120 ggatgcagag tcccagcttg gtgccagagt tagtggaatc gttcacgctt ctggtgtgct 18180 ccgtgatcgt ctcatagaga agaaactccc tgacgagttt gatgctgtct ttgggaccaa 18240 agtcactggg ttggagaatc ttctggctgc agttgatcgt gccaacctca aacacatggt 18300 tctcttcagc tcccttgctg gtttccacgg caatgttggt cagtctgact atgcgatggc 18360 aaacgaagct ctcaacaaga tgggtttgga acttgcgaag gatgtcagtg tcaagtccat 18420 ctgctttggt ccctgggatg gtgggatggt gacacctcaa ctcaagaaac agtttcaaga 18480 aatgggagtt cagatcattc ctcgtgaagg aggtgctgat acagttgcga ggattgtgtt 18540 gggatcaagt cctgctgaga tccttgttgg aaactggagg acaccatcca agaaagttgg 18600 aagtgacacg atcaccctgc atagaaagat cagtgccaag tccaatccct tcttggagga 18660 tcatgtcata caaggcagaa gggtgttgcc aatgaccctt gctattggga gccttgctga 18720 gacctgcctt ggactcttcc ctggctactc tctctgggcc atagatgacg cacagctctt 18780 caaaggtgtc actgttgatg gagacgtcaa ctgtgaagtg accctcacac cgtccactgc 18840 tccgagtggg agggtgaatg tccaagcgac gctcaagacg ttctcaagtg gaaaacttgt 18900 tccagcctac agagctgtca tagtgttgtc caaccaaggt gctccacctg ccaatgcaac 18960 catgcagcct ccgagccttg atgcagatcc tgctttgcaa gggtcagtct atgatggcaa 19020 gactctcttt catggtcctg cgttcagagg cattgatgac gttctcagct gcaccaagag 19080 ccagcttgtt gccaagtgtt ctgctgttcc tggatcagat gcagccagag gagagtttgc 19140 aactgacact gatgcacatg atccctttgt caatgatctt gcctttcaag caatgttggt 19200 ctgggtgagg agaacgctgg gacaagctgc actcccgaac tccattcaga ggatagtcca 19260 acacagaccg gtcccacaag acaaaccctt ctacatcacc ctcagatcca atcagagtgg 19320 tggacatagc cagcacaaac atgcacttca gttccacaac gaacaaggtg atctcttcat 19380 tgatgttcaa gcctctgtca ttgcgacaga ctcacttgcg ttctgagtag ttagcttaat 19440 cacttaggtc acccagtcca agcccggcct agcacgatga tcatgagtgt tgctattgat 19500 gtatgttaat actatacaca gtcatcgtgt gtgttttata aaactgtaag cttaggatgt 19560 ttgaggctaa tgtaattagc actacaccgt aataaatgag aggtttactt tatgttttaa 19620 taatgttctt acccttgctg ctctctaaac tgtaatggcg tacggttagc taaactaaag 19680 taaaaatcga ttcgcttgag taaaattagt gaccgggaaa gatcatcgac tactactgct 19740 cagagatgta atcggtaatt aagacttcaa gctcaataat taattaggag taaatctggc 19800 ttcgaatttt taaattgtca acttccaaag aaggattggc aagactcaat ctcaattgcc 19860 caacaaacat atggctcttt acaaatttgg aatctctttt tgatggcaag aacgtcgtaa 19920 ttcctctgtc gatctcatgt aattaccaaa ctcatctact tattactttc tttatatgca 19980 cttactgatg cggccgctta attaagatga gtgatactca ggactcagga ctcactctgc 20040 tgatcactag tgctagcctc gaggtcgaca caactttgta tacaaaagtt gtctagacct 20100 aggctcagca actcatccag tcaagttcga gtcagtgagt tgggcggccg cagcttatag 20160 taaagatttt tttgttaata aaatgaaagt taatatttat ggatttattt gaacatattt 20220 acaagttttt gttaataaat ttatttacag aagtgttaaa tttgaaaatg tgttaaatga 20280 gagttaatat ttatggatat aatattactt tatttaaaaa taaaatgaat ttatattaaa 20340 atatgatttg gtaaaagata ttataaagag gaacttggat gagaaagacg acgatgggca 20400 aaagttgcat ggcacaccgc ttttgttcag taacatctgc actgtggcgc ctttttcaca 20460 ctcaccatgc atgctgccac gtcagctttc acactatgac accactggcc atacacgttg 20520 ccacctcagc tttctcctct tctcactatg acacgactgg ccatgcatgc atgcatgctg 20580 ccacctcagc tcccgcctct tcacccgtgt ctttctctca tgtcgcagtg ctgcgtgcca 20640 acctcttctc tctataaata gagagaagag aatgatggtt aaactcaaac caaaacaaga 20700 aaacatacac aaatagcaaa acggatccac aaccaaaaat ggcttcctct atgctctctt 20760 ccgctactat ggttgcctct ccggctcagg ccactatggt cgctcctttc aacggactta 20820 agtcctccgc tgccttccca gccacccgca aggctaacaa cgacattact tccatcacaa 20880 gcaacggcgg aagagttaac tgcatgcagg tgtggcctcc gattggaaag aagaagtttg 20940 agactctctc ttaccttcct gaccttaccg attccgaagc tgcaaggaac gtgagtgcgg 21000 ctcatgagat gcatgacgag aagaggatag ctgttgtggg catggctgtt cagtatgctg 21060 gatgcaaaac gaaagacgag ttctgggaag ttctcatgaa tggaaaagtt gagagcaaag 21120 tgatcagcga caaacgtctt ggttcaaatt accgtgctga acactacaaa gctgaaagga 21180 gcaagtatgc tgacaccttc tgcaatgaga catatggaac tcttgatgag aacgaaatag 21240 acaatgagca tgaactcctt ttgaaccttg ccaaacaagc cttggctgaa acatctgtca 21300 aagattcaac aagatgtggc atagtttctg gatgcctcag cttcccgatg gacaacctcc 21360 aaggtgaact cttgaatgtg taccagaacc atgttgagaa gaaacttggt gccagagttt 21420 tcaaagatgc ctctcactgg tcagaaagag aacagtccaa caaaccagag gctggtgaca 21480 gaagaatctt catggaccct gcttcctttg ttgctgagga actcaatctt ggcgctctcc 21540 actactctgt tgatgctgcc tgtgccactg cgctctatgt gctcagactg gcgcaagatc 21600 acttggtctc tggtgcagct gatgtgatgc tttgtggtgc aacatgcctg ccggagccgt 21660 ttttcatact ctctgggttc tcaaccttcc aagccatgcc tgttggaacg ggtcagaatg 21720 tgtccatgcc gctgcacaaa gattctcaag gattgacccc tggtgaagga ggttccatca 21780 tggtcctcaa acgtttggat gacgcaatca gagatggaga tcacatctat ggaacacttt 21840 tgggagccaa tgtcagcaac agtggcactg gtctgccact taaacccctt ttgccgtcag 21900 agaaaaagtg cctcatggac acctacacac gtatcaatgt gcatccccac aagattcagt 21960 atgttgaatg ccatgcaact gggactccac aaggtgatcg tgtggaaatt gatgctgtga 22020 aggcttgctt tgaaggcaaa gtcccacgtt ttggaaccac aaaagggaac tttggacaca 22080 cattggtggc agctgggttt gctggcatgt gcaaagtgct tctctccatg aagcacggga 22140 tcattccacc cactccggga atagatgacg aaacgaagat ggaccctctg gttgtctctg 22200 gtgaggccat tccctggcca gaaacgaatg gagaaccgaa gcgtgctggt ctctcagcgt 22260 ttggatttgg tgggaccaat gctcatgctg tctttgagga acatgaccca agcaatgctg 22320 catgcactgg ccatgactcc atttctgctc tctctgcacg ttgtggtggt gagtcaaaca 22380 tgagaatagc aatcactggg atggatgcca cttttggtgc tttgaaagga ttggatgctt 22440 ttgaaagggc catctacact ggtgctcatg gtgctatacc actcccagaa aagagatgga 22500 gattcctggg gaaagacaag gacttccttg atctttgtgg agtcaaagca acaccgcatg 22560 gctgttacat tgaagatgtt gaagttgatt tccagaggct gaggactcca atgactcctg 22620 aagatatgct cttgcctcaa cagcttctgg ctgtcaccac aattgatagg gccatattgg 22680 actctggcat gaagaaaggt ggaaatgtgg ctgtctttgt ggggttgggg accgatcttg 22740 aactctaccg tcaccgtgca agggttgctc tcaaggagag agtgaggcct gaagccagca 22800 agaaactcaa tgacatgatg cagtacatca atgattgtgg gacaagcact tcttacacca 22860 gctacatagg caacttggtt gcaacaaggg tcagttcaca atggggcttc acgggacctt 22920 ccttcacaat cacagaaggc aacaattctg tgtacagatg tgctgaactt ggcaaatacc 22980 tcttggaaac gggagaggtt gatggggttg tggttgcggg tgttgatctc tgtgggagtg 23040 ctgagaacct ttacgtcaag tcaaggcgtt tcaaggtgtc tacatctgac accccgagag 23100 ccagctttga tgcagctgct gatggttact ttgttggaga gggctgtggt gcatttgtgc 23160 tcaagcgtga gacaagctgc accaaggatg acagaatcta tgcttgcatg gatgccatag 23220 ttcctggaaa tgttccatct gcatgtttga gagaggcatt ggatcaagcc agagtcaagc 23280 ctggagacat cgagatgttg gagctttctg ctgattcagc gaggcatttg aaggacccgt 23340 ctgttctccc aaaggaactc actgctgagg aagagattgg cggacttcag acgattcttc 23400 gtgacgatga caagctccca aggaatgttg caactggaag tgtcaaggcc actgttggtg 23460 acactggtta tgcttctggt gctgccagtt tgattaaggc tgcgctttgc atctacaacc 23520 gttacctgcc cagcaatgga gatgactggg atgaacctgc acctgaagca ccctgggaca 23580 gcacactctt tgcgtgccag acctcaagag cttggttgaa gaaccctggt gagagacgtt 23640 atgcggctgt ttccggagtc agtgaaacca gaagttgcta cagtgtgctg ctttctgaag 23700 cagagggaca ctacgagaga gagaacagaa tcagtttgga tgaggaagca cccaagctca 23760 ttgtgttgag agcagactcc catgaagaga ttcttggaag attggacaag ataagggaaa 23820 ggttcttgca gcccacgggt gctgcaccca gagagtctga attgaaggcg caagcccgta 23880 ggatctttct tgaactcctt ggtgagaccc ttgcccaaga tgcagcttca tctggaagtc 23940 agaagcccct tgctctcagc ttggtctcca ctccatccaa gctccagaga gaggtggaac 24000 ttgcggcaaa aggcattccg agatgtctca agatgagacg tgattggagt tcccctgctg 24060 gaagcagata tgcgcctgag cctttggcca gtgacagagt tgccttcatg tatggagaag 24120 gaaggagccc ttactatggc atcacacaag acattcatag gatttggcct gaacttcatg 24180 aggtcatcaa tgagaaaaca aaccgtctct gggctgaagg tgacagatgg gtcatgccaa 24240 gggccagctt caagagtgag cttgagtcac aacagcaaga gtttgacaga aacatgattg 24300 aaatgttccg tcttggaatc ctcacctcaa ttgccttcac aaacctggcg agagatgttc 24360 tcaacatcac gcccaaagca gcctttggac tcagtttggg agagatttcc atgatctttg 24420 ctttctccaa gaaaaacggt ttgataagcg atcagttgac aaaggatttg agagagtcag 24480 atgtctggaa caaagctctg gctgttgagt tcaatgcgct cagagaagcc tggggcattc 24540 cacagtctgt cccaaaagat gagttctggc aaggttacat agtgagggga acgaaacaag 24600 acatagaagc agccatagca ccagactcaa agtatgtgag gttgaccatc atcaatgatg 24660 ccaacactgc tctcatcagt ggaaagcctg atgcatgcaa agctgcgatt gctcgtcttg 24720 gtggcaacat ccctgcgctt ccagtcactc aaggcatgtg tgggcattgc ccagaggtgg 24780 gaccttacac caaggacatc gcaaagatcc acgccaacct tgagttccct gtggttgatg 24840 gtcttgatct ctggactacc ataaaccaga agaggcttgt gccaagagcc acgggtgcca 24900 aagatgaatg ggctccttca tcctttgggg agtatgctgg acagctctat gagaaacaag 24960 ccaacttccc tcagatagtt gaaaccatct acaagcagaa ctatgatgtg tttgttgaag 25020 ttgggccaaa caatcacaga agcacagcag tgagaaccac gcttggacca cagagaaacc 25080 accttgctgg tgccatagac aagcagaacg aggatgcttg gacgacaata gtcaagctcg 25140 tggcttcatt gaaggctcac cttgttcctg gagtgaccat cagtccgctc taccactcca 25200 agctcgttgc agaggctgag gcttgttatg ctgcgctctg taaaggtgag aaacccaaga 25260 aaaacaagtt tgtgagaaag attcagttga atggcagatt caacagcaag gcagacccca 25320 tctcctcagc agaccttgca tccttcccac ctgcggaccc tgcgattgaa gctgccatct 25380 cttcaaggat catgaagcct gtggctccga aattctatgc gcgtctcaac attgatgagc 25440 aagatgagac cagagatccg atcttgaaca aggacaatgc gccttccagc tccagctctt 25500 ccagcagttc ctcttccagc agttcatctc cgtctcctgc tccttcagct ccagttcaga 25560 agaaagctgc cccagctgcg gagacaaaag ctgttgccag tgctgatgca cttcgtagtg 25620 ccctgcttga tcttgactcc atgcttgcgc tcagctctgc aagtgcttct ggcaaccttg 25680 ttgagactgc gccatctgat gccagtgtca ttgtccctcc gtgcaacatt gctgatcttg 25740 gcagcagagc tttcatgaaa acttatggtg tttctgcgcc tctgtacacc ggtgcaatgg 25800 ccaagggaat tgcctctgcg gacttggtga tcgcagctgg aaggcaagga atccttgcgt 25860 catttggtgc tggaggcctt ccaatgcaag ttgtgcgtga gtccattgaa aagattcaag 25920 ctgccctgcc caatggacca tatgctgtca acctcatcca ttctcctttt gacagcaacc 25980 ttgagaaggg aaatgttgat ctcttcttgg aaaagggtgt cacttttgtt gaggcttcag 26040 ccttcatgac gttgacaccg caagttgtca gatacagagc ggctgggttg acgaggaatg 26100 cagatgggtc agtcaacata aggaacagaa tcattgggaa agtctccaga actgagctgg 26160 ctgaaatgtt catgcgtcct gcacctgagc atcttttgca gaagctcatt gcttctgggg 26220 agatcaatca agaacaagct gagttggcca gacgtgttcc tgtggctgat gacattgcgg 26280 ttgaagctga ttctggtgga cacactgaca accgtccaat ccatgtcatt ctgcctctga 26340 ttatcaacct gagggatagg cttcacagag agtgtggata cccagcaaac ctgagggttc 26400 gtgttggtgc tgggggtggc attgggtgtc cccaagctgc gctggcaact ttcaacatgg 26460 gtgcctcttt cattgtcact ggaacagtga accaagttgc caaacaatct gggacgtgtg 26520 acaatgtgag gaaacagctt gcgaaagcaa cttactcaga tgtttgcatg gcaccggcag 26580 ctgacatgtt tgaggaaggt gtgaaacttc aagttttgaa gaaaggaaca atgttccctt 26640 caagagccaa caaactctat gagttgttct gcaagtatga ttcttttgag tccatgcctc 26700 cagcagagct tgcgagggtt gagaaaagaa tcttcagtcg tgcattggaa gaggtctggg 26760 atgagaccaa gaacttctac atcaaccgtc tccacaaccc agagaagata cagagggctg 26820 agagggaccc caaactgaag atgtcactgt gtttcagatg gtatctgtcc ctggcttcaa 26880 ggtgggccaa cactggagct tctgacagag tcatggacta ccaagtttgg tgtggtcctg 26940 ccattggttc attcaatgat ttcatcaaag gaacttacct tgatccggca gtggcaaatg 27000 agtacccgtg tgttgtgcag atcaacaaac agattcttag aggagcgtgc ttcttgaggc 27060 gtcttgaaat actgagaaat gcaaggcttt cagatggtgc agctgcactt gtggcgagca 27120 ttgatgacac atatgtccct gctgagaagc tgtgagtagt tagcttaatc acttaggtca 27180 cccagtccaa gcccggccta gcacgatgat catgagtgtt gctattgatg tatgttaata 27240 ctatacacag tcatcgtgtg tgttttataa aactgtaagc ttaggatgtt tgaggctaat 27300 gtaattagca ctacaccgta ataaatgaga ggtttacttt atgttttaat aatgttctta 27360 cccttgctgc tctctaaact gtaatggcgt acggttagct aaactaaagt aaaaatcgat 27420 tcgcttgagt aaaattagtg accgggaaag atcatcgact actactgctc agagatgtaa 27480 tcggtaatta agacttcaag ctcaataatt aattaggagt aaatctggct tcgaattttt 27540 aaattgtcaa cttccaaaga aggattggca agactcaatc tcaattgccc aacaaacata 27600 tggctcttta caaatttgga atctcttttt gatggcaaga acgtcgtaat tcctctgtcg 27660 atctcatgta attaccaaac tcatctactt attactttct ttatatgcac ttactgatgc 27720 ggccgcttaa ttaatctgac ctgaccacta gacctgagtg agtacagatg tgctactagt 27780 gctagcctcg aggtcgacca acttttctat acaaagttgt ctagacctag gactaggcac 27840 tgagtgactc actgacctga ctgagtgact ggcggccgca gcttatagta aagatttttt 27900 tgttaataaa atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt 27960 taataaattt atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt 28020 atggatataa tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt 28080 aaaagatatt ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg 28140 cacaccgctt ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat 28200 gctgccacgt cagctttcac actatgacac cactggccat acacgttgcc acctcagctt 28260 tctcctcttc tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc 28320 ccgcctcttc acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc 28380 tataaataga gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa 28440 atagcaaaac ggatccacaa ccaaaaatgg cttcctctat gctctcttcc gctactatgg 28500 ttgcctctcc ggctcaggcc actatggtcg ctcctttcaa cggacttaag tcctccgctg 28560 ccttcccagc cacccgcaag gctaacaacg acattacttc catcacaagc aacggcggaa 28620 gagttaactg catgcaggtg tggcctccga ttggaaagaa gaagtttgag actctctctt 28680 accttcctga ccttaccgat tccgaagcgc ttcgtgtcaa gaccaacaag aagccatgtt 28740 gggagatgac caaggaggaa ctgacctctg gcaaaactga ggtgttcaac tatgaggaac 28800 tcctggagtt tgcagagggt gacatagcta aggtttttgg tccggagttt gctgtcatag 28860 acaagtaccc cagaagggtg aggctgcctg ctcgtgaata cctgcttgtg acaagggtca 28920 cactcatgga tgccgaggtc aacaactaca gagttggtgc cagaatggtc actgagtatg 28980 atcttccagt gaatggcgaa ttgtctgagg gaggtgattg tccctgggca gttctggttg 29040 agagtggaca gtgcgatttg atgctgatat catacatggg aattgatttc cagaaccaag 29100 gtgacagagt ttaccgtctg ctcaacacca cgttgacatt ctatggggtt gcccatgagg 29160 gagagaccct tgagtatgac atcagagtga ccggttttgc caaacgtttg gatggtggca 29220 tctccatgtt cttctttgag tatgactgct atgtcaatgg gaggcttctc attgagatga 29280 gagatggctg tgctggcttc ttcacaaatg aggagcttga tgcgggaaag ggtgtggtgt 29340 tcacaagagg ggatttggct gccagagcta agattccaaa gcaagatgtt tctccctatg 29400 ctgtcgctcc ctgccttcac aaaacaaaac tcaacgaaaa ggagatgcag accttggttg 29460 acaaagattg ggcttccgtt tttggctcaa agaatggcat gccggagatc aactacaaac 29520 tctgcgcacg taagatgctc atgattgata gagtcaccag cattgaccat aagggaggtg 29580 tgtacggttt gggtcagttg gttggtgaga aaatcttgga gagggaccat tggtacttcc 29640 catgccattt tgttaaggat caagtcatgg ctggatctct tgtcagcgac ggttgttcac 29700 aaatgctcaa gatgtacatg atctggctcg gtttgcacct taccaccgga ccatttgact 29760 tcagacccgt caacggacac ccgaacaaag ttaggtgtag gggtcagata tctcctcata 29820 agggaaagtt ggtctacgtc atggagatca aagagatggg attcgacgag gataacgatc 29880 cgtacgccat tgctgacgtc aacatcattg acgttgattt cgaaaagggt caagatttca 29940 gccttgatag aatcagcgac tacggaaagg gggatcttaa caaaaagatc gtcgtggact 30000 tcaagggcat tgctctcaag atgcagaaga gatccacgaa caagaacccc tccaaagttc 30060 agcccgtttt tgctaacggt gcagccactg tgggaccgga agcgtccaag gccagctctg 30120 gtgccagtgc tagtgcttct gctgcccctg cgaagcctgc tttctcagca gatgttcttg 30180 cgccaaagcc agttgcactt cctgaacata tcctcaaggg tgacgctctc gctccgaaag 30240 aaatgtcctg gcatcccatg tcaaaacttc ctggcaaccc gacgccgttc ttctccccta 30300 gttcttaccc tccgagggca atttgcttca tccctttccc tggcaaccca cttgacaaca 30360 attgcaaagc tggagaaatg cctctgaact ggtacaacat gtcagagttc atgtgtggaa 30420 aagttagcaa ctgcttggga ccagagttcg caagatttga taagtcaaat acgagcagaa 30480 gccctgcttt tgatttggct cttgtgacta gagttgttga agtcacaaac atggaacatg 30540 gcaagttcct gaatgttgat tgcaatccca gcaaaggcac aatggtgggg gagtttgact 30600 gtcctcaaga tgcgtggttc tttgatggtt cttgcaatga tgggcacatg ccttattcca 30660 tcatcatgga aatcggactt cagacatccg gtgttctcac cagtgtgttg aaagcaccac 30720 tgacgatgga caaagatgac attctcttta ggaaccttga tgcaagtgct gaaatggtga 30780 ggccagatgt ggatgtgagg gggaaaacca taaggaatgt gaccaagtgc actggatatg 30840 caatgttggg aaagatgggg attcacagat tcacgtttga gttgtctgtt gatggcgtgg 30900 ttttctacaa aggaagcact tcctttggat ggttcactcc agaggtgttt gctcagcaag 30960 ctggacttga caatgggaaa aagacggagc cttggtgcaa aactaacaac acgagtgtga 31020 gaagagttga aatcgcatca gccaaaggca aagaacagct gactgagaaa cttccagatg 31080 caactaatgc tcaagttctc agaaggtcag aacagtgtga gtatttggat tacctcaaca 31140 ttgctcctga ttctgggctg catgggaaag gatatgcgca tggacacaaa gatgttaacc 31200 cacaagactg gttcttctct tgtcactttt ggtttgatcc tgtgatgcct ggatctttgg 31260 gaattgagtc aatgttccag ctcattgagg catttgcggt ggaccagaac ataccgggag 31320 aatacaatgt gagcaatccg acctttgccc atgcccctgg aaaaacggcg tggaagtacc 31380 gtggtcaatt gacaccaaag aaccgtgcga tggactgtga ggtgcacata gtttcaatca 31440 cagcctctcc tgaaaatgga ggctacgttg acatagtggc agatggagca ctttgggttg 31500 atggtctccg tgtgtatgaa gccaaagaac tcagagttcg tgttgtcagt gcaaaacctc 31560 aagccattcc ggatgttcaa caacagccac ccagcgcaaa agcggaccct gggaaaactg 31620 gagttgcact cagtccgact cagttgagag atgtcctgct tgaagtggac aatccattgt 31680 atcttggtgt tgaaaactcc aatttggtgc agtttgaatc caaacctgca acttcttcaa 31740 ggattgtcag tatcaaacca tgctccataa gtgatcttgg tgacaagtcc ttcatggaaa 31800 cgtacaatgt gtcagcacct ctgtacactg gagcaatggc caagggaatt gcgtctgcgg 31860 accttgtcat tgctgctggg aagaggaaga tattgggttc ttttggagcg ggtgggcttc 31920 caatgcacca tgtgagggca gctttggaga agatacaagc ggctctgcct caaggaccat 31980 atgctgtgaa cctcattcat tcaccttttg acagcaactt ggaaaagggg aatgttgatc 32040 tcttcctcga aaagggcgtt actgtggtgg aggcttctgc attcatgacc ctcactcccc 32100 aagttgttcg ttaccgtgca gccggactct caaggaacgc tgatgggagt gtcaacatca 32160 gaaaccgtat cattggaaaa gtgtccagaa cagaactcgc cgaaatgttc atcagaccgg 32220 ctccggaaca cctccttgag aaactcatag cttctggtga gataacacaa gaacaagccg 32280 aacttgcccg tcgtgttcct gtcgcagacg acatcgctgt tgaagctgat tctggtggtc 32340 acactgacaa ccgtcccatc cacgtcatct tgcctttgat catcaacctt aggaacagac 32400 tgcacagaga gtgtggttat cctgcgcacc ttagagtcag agttggagct ggaggcggtg 32460 ttggctgccc acaagctgct gcggctgccc tcacaatggg agctgccttc atagtgactg 32520 ggactgtcaa tcaagttgct aaacagtccg ggacatgtga caacgtgaga aaacaactca 32580 gtcaagccac ctacagtgac atttgcatgg ctccagcagc tgatatgttt gaggaaggtg 32640 tgaaactcca agttctcaag aagggaacaa tgttcccatc aagagctaac aaattgtacg 32700 aactcttttg caagtacgac tctttcgatt caatgcctcc tgctgaactt gaaaggatcg 32760 agaagaggat cttcaagagg gcacttcaag aggtctggga agagaccaag gacttttaca 32820 tcaatggttt gaagaatcca gagaagatac agagagcgga acatgacccc aaactcaaga 32880 tgtctttgtg tttcagatgg tatcttggtc ttgcatcacg ttgggccaac atgggtgctc 32940 ctgatcgtgt catggactat caagtctggt gtggtccagc cattggagcc ttcaatgact 33000 tcatcaaagg aacatatctt gatcctgctg tctctaatga gtacccatgt gttgtccaga 33060 tcaaccttca gattttgcgt ggtgcatgtt acctgaggcg tcttaatgct ctgagaaatg 33120 atccgaggat tgatcttgag actgaagatg ctgcctttgt ctatgagcca acgaatgcgc 33180 tttgagtagt tagcttaatc acttaggtca cccagtccaa gcccggccta gcacgatgat 33240 catgagtgtt gctattgatg tatgttaata ctatacacag tcatcgtgtg tgttttataa 33300 aactgtaagc ttaggatgtt tgaggctaat gtaattagca ctacaccgta ataaatgaga 33360 ggtttacttt atgttttaat aatgttctta cccttgctgc tctctaaact gtaatggcgt 33420 acggttagct aaactaaagt aaaaatcgat tcgcttgagt aaaattagtg accgggaaag 33480 atcatcgact actactgctc agagatgtaa tcggtaatta agacttcaag ctcaataatt 33540 aattaggagt aaatctggct tcgaattttt aaattgtcaa cttccaaaga aggattggca 33600 agactcaatc tcaattgccc aacaaacata tggctcttta caaatttgga atctcttttt 33660 gatggcaaga acgtcgtaat tcctctgtcg atctcatgta attaccaaac tcatctactt 33720 attactttct ttatatgcac ttactgatgc ggccgcttaa ttaatcacga gtggatgact 33780 gtcacgactg actcatgact gactactagt gctagcctcg aggtcgacac aactttgtat 33840 aataaagttg tctagaccta ggagctgtgc tgggtaactc acatgagtca ctggtcacat 33900 cagcggccgc agcttatagt aaagattttt ttgttaataa aatgaaagtt aatatttatg 33960 gatttatttg aacatattta caagtttttg ttaataaatt tatttacaga agtgttaaat 34020 ttgaaaatgt gttaaatgag agttaatatt tatggatata atattacttt atttaaaaat 34080 aaaatgaatt tatattaaaa tatgatttgg taaaagatat tataaagagg aacttggatg 34140 agaaagacga cgatgggcaa aagttgcatg gcacaccgct tttgttcagt aacatctgca 34200 ctgtggcgcc tttttcacac tcaccatgca tgctgccacg tcagctttca cactatgaca 34260 ccactggcca tacacgttgc cacctcagct ttctcctctt ctcactatga cacgactggc 34320 catgcatgca tgcatgctgc cacctcagct cccgcctctt cacccgtgtc tttctctcat 34380 gtcgcagtgc tgcgtgccaa cctcttctct ctataaatag agagaagaga atgatggtta 34440 aactcaaacc aaaacaagaa aacatacaca aatagcaaaa cggatccaca accaaaaatg 34500 gcttcctcta tgctctcttc cgctactatg gttgcctctc cggctcaggc cactatggtc 34560 gctcctttca acggacttaa gtcctccgct gccttcccag ccacccgcaa ggctaacaac 34620 gacattactt ccatcacaag caacggcgga agagttaact gcatgcaggt gtggcctccg 34680 attggaaaga agaagtttga gactctctct taccttcctg accttaccga ttccgaactt 34740 cagcacactt ggcttccgaa gcctcccaat ctgaccctct tgtcagatga ggttcatctc 34800 tggaggattc ctcttgacca gcctgagtca caacttcaag accttgctgc caccctgagc 34860 agtgatgaat tggcgagggc aaacagattc tactttccag aacacagaag gcgtttcact 34920 gctgggagag gcatcctcag atccatcttg ggtggatact tgggagtgga accgggtcaa 34980 gtcaagtttg attatgagtc ccgtgggaaa ccgatccttg gtgacagatt tgctgagagt 35040 ggactcctgt tcaacttgtc tcacagccag aaccttgcct tgtgtgctgt caactacacg 35100 cgtcaaatag gcattgatct tgaatatctg cgtccaacat ctgacttgga gtctcttgca 35160 aagaggttct ttctcccaag agaatatgaa ctcttgaggt cactccctga tgagcagaaa 35220 cagaagattt tctttcgtta ctggacttgc aaagaggctt atctcaaagc aacgggagat 35280 ggaatagcca aacttgaaga gatcgagata gcactcaccc caacagaacc tgccaagctc 35340 caaacagctc ctgcgtggtc tctgttggag cttgtgccag atgacaattg tgttgcagct 35400 gtggctgttg cgggttttgg ttggcagccc aagttctggc attactgagt agttagctta 35460 atcacttagg tcacccagtc caagcccggc ctagcacgat gatcatgagt gttgctattg 35520 atgtatgtta atactataca cagtcatcgt gtgtgtttta taaaactgta agcttaggat 35580 gtttgaggct aatgtaatta gcactacacc gtaataaatg agaggtttac tttatgtttt 35640 aataatgttc ttacccttgc tgctctctaa actgtaatgg cgtacggtta gctaaactaa 35700 agtaaaaatc gattcgcttg agtaaaatta gtgaccggga aagatcatcg actactactg 35760 ctcagagatg taatcggtaa ttaagacttc aagctcaata attaattagg agtaaatctg 35820 gcttcgaatt tttaaattgt caacttccaa agaaggattg gcaagactca atctcaattg 35880 cccaacaaac atatggctct ttacaaattt ggaatctctt tttgatggca agaacgtcgt 35940 aattcctctg tcgatctcat gtaattacca aactcatcta cttattactt tctttatatg 36000 cacttactga tgcggccgct taattaacta ctgtcactga ggccgtagac gagtacggac 36060 tgatctaact agtgctagcc tcgaggtcga cacccagctt tc 36102 <210> 43 <211> 39183 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB100518 <400> 43 ttgtacaaag tggttgcggc cgcttaatta aatttaaatt caattaatgc aatcttgatt 60 ttcaacaacg aaggtaatgg cgtaaaagaa aaaatgtatg ttattgtatt gatctttcat 120 gatgttgaag cgtgccataa tatgatgatg tataattaaa atattaactg tcgcatttta 180 ttgaaatggc actgttattt caaccatatc tttgattctg ttacatgaca cgactgcaag 240 aagtaaataa tagacgccgt tgttaaagaa ttgctatcat atgtgcctaa ctagagggaa 300 tttgagcgtc agacctaatc aaatattaca aaatatctca ctctgtcgcc agcaatggtg 360 taatcagcgc agacaaatgg cgtaaagatc gcggaaaaac ctccccgagt ggcatgatag 420 ctgcctctgt attgctgatt tagtcagcct tatttgactt aagggtgccc tcgttagtga 480 caaattgctt tcaaggagac agccatgccc cacactttgt tgaaaaacaa attgcctttg 540 gggagacggt aaagccagtt gctcttcaat aaggaatgtc gaggaggcaa tgtaaccgcc 600 tctggtagta cacttctcta atccaaaaat caatttgtat tcaagatacc gcaaaaaact 660 tatggtttaa accctgcagg actagtccag aaggtaatta tccaagatgt agcatcaaga 720 atccaatgtt tacgggaaaa actatggaag tattatgtaa gctcagcaag aagcagatca 780 atatgcggca catatgcaac ctatgttcaa aaatgaagaa tgtacagata caagatccta 840 tactgccaga atacgaagaa gaatacgtag aaattgaaaa agaagaacca ggcgaagaaa 900 agaatcttga agacgtaagc actgacgaca acaatgaaaa gaagaagata aggtcggtga 960 ttgtgaaaga gacatagagg acacatgtaa ggtggaaaat gtaagggcgg aaagtaacct 1020 tatcacaaag gaatcttatc ccccactact tatcctttta tatttttccg tgtcattttt 1080 gcccttgagt tttcctatat aaggaaccaa gttcggcatt tgtgaaaaca agaaaaaatt 1140 tggtgtaagc tattttcttt gaagtactga ggatacaact tcagagaaat ttgtaagttt 1200 gtaggtacca gatctggatc ccaaaccatg tctccggaga ggagaccagt tgagattagg 1260 ccagctacag cagctgatat ggccgcggtt tgtgatatcg ttaaccatta cattgagacg 1320 tctacagtga actttaggac agagccacaa acaccacaag agtggattga tgatctagag 1380 aggttgcaag atagataccc ttggttggtt gctgaggttg agggtgttgt ggctggtatt 1440 gcttacgctg ggccctggaa ggctaggaac gcttacgatt ggacagttga gagtactgtt 1500 tacgtgtcac ataggcatca aaggttgggc ctaggatcta cattgtacac acatttgctt 1560 aagtctatgg aggcgcaagg ttttaagtct gtggttgctg ttataggcct tccaaacgat 1620 ccatctgtta ggttgcatga ggctttggga tacacagccc ggggtacatt gcgcgcagct 1680 ggatacaagc atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca 1740 gctcctccaa ggccagttag gccagttacc caaatctgag tagttagctt aatcacctag 1800 agctcgatcg gcggcaatag cttcttagcg ccatcccggg ttgatcctat ctgtgttgaa 1860 atagttgcgg tgggcaaggc tctctttcag aaagacaggc ggccaaagga acccaaggtg 1920 aggtgggcta tggctctcag ttccttgtgg aagcgcttgg tctaaggtgc agaggtgtta 1980 gcgggatgaa gcaaaagtgt ccgattgtaa caagatatgt tgatcctacg taaggatatt 2040 aaagtatgta ttcatcacta atataatcag tgtattccaa tatgtactac gatttccaat 2100 gtctttattg tcgccgtatg taatcggcgt cacaaaataa tccccggtga ctttctttta 2160 atccaggatg aaataatatg ttattataat ttttgcgatt tggtccgtta taggaattga 2220 agtgtgcttg aggtcggtcg ccaccactcc catttcataa ttttacatgt atttgaaaaa 2280 taaaaattta tggtattcaa tttaaacacg tatacttgta aagaatgata tcttgaaaga 2340 aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc caagcaaaaa 2400 cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat tatatcagct 2460 ggtacattgc cgtagatgaa agactgagtg cgatattatg gtgtaataca taggaattcg 2520 tttaaacgat ctgcgtctaa ttttcggtcc aacttgcaca ggaaagacgt cgaccgcggt 2580 agctcttgcc cagcagactg ggcttccagt cctttcgctc gatcgggtcc aatgttgtcc 2640 tcagctgtga accggaagcg gacgaccaac agtggaagaa ctgaaaggaa cgagccgtct 2700 ataccttgat gatcggcctc tggtgaaggg tatcatcgca gccaagcaag ctcatgaaag 2760 gctgatgggg gaggtgtata attatgaggc ccacggcggg cttattcttt agggaggatc 2820 tatctcgttg ctcaagtgca tggcgcaaag cagttattgg agtgcggatt ttcgttggca 2880 tattattcgc cacgagttag cagacgaaga gaccttcatg aacgtggcca aggccagagt 2940 taagcagatg ttacgccctg ctgcaggcct ttctattatc caatagttgg ttgatctttg 3000 gaaagagcct cggctgaggc ccatactgaa agagatcgat ggatatcgat atgccatgtt 3060 gtttgctagc cagaaccaga tcacatccga tatgctattg cagcttgacg cagatatgga 3120 ggataagttg attcatggga tcgctcagga gtagctcatc catgcacgcc gacaagaaca 3180 gaaattccgt cgagttaacg cagccgctta cgacggattc gaaggtcatc cattcggaat 3240 gtattagttt gcaccagctc cgcgtcacac ctgtcttcat ttgaataaga tgttagcaat 3300 tgtttttagc tttgtcttgt tgtggcaggg cggcaagtgc ttcagacatc attctgtttt 3360 caaattttat gctggagaac agcttcttaa ttcctttgga aataatagac tgcgtcttaa 3420 aattcagatg tctggatata gatatgattg taaaataacc tatttaagtg tcatttagaa 3480 cataagtttt atgaatgttc ttccattttc gtcatcgaac gaataagagt aaatacacct 3540 tttttaacat tacaaataag ttcttatacg ttgtttatac accgggaatc atttccatta 3600 ttttcgcgca aaagtcacgg atattcgtga aagcgacata aactgcgaaa tttgcgggga 3660 gtgtcttgag tttgcctcga ggctagcgca tgcacataga cacacacatc atctcattga 3720 tgcttggtaa taattgtcat tagattgttt ttatgcatag atgcactcga aatcagccaa 3780 ttttagacaa gtatcaaacg gatgtgactt cagtacatta aaaacgtccg caatgtgtta 3840 ttaagttgtc taagcgtcaa tttgatttac aattgaatat atcctgcccc agccagccaa 3900 cagctcgatt tacaattgaa tatatcctgc cggccggccc acgcgtgtcg aggaattctg 3960 atctggcccc catttggacg tgaatgtaga cacgtcgaaa taaagatttc cgaattagaa 4020 taatttgttt attgctttcg cctataaata cgacggatcg taatttgtcg ttttatcaaa 4080 atgtactttc attttataat aacgctgcgg acatctacat ttttgaattg aaaaaaaatt 4140 ggtaattact ctttcttttt ctccatattg accatcatac tcattgctga tccatgtaga 4200 tttcccggac atgaagccat ttacaattga atatatcctg ccgccgctgc cgctttgcac 4260 ccggtggagc ttgcatgttg gtttctacgc agaactgagc cggttaggca gataatttcc 4320 attgagaact gagccatgtg caccttcccc ccaacacggt gagcgacggg gcaacggagt 4380 gatccacatg ggacttttaa acatcatccg tcggatggcg ttgcgagaga agcagtcgat 4440 ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg atcgcaaagt atttgaacgc 4500 aggtacaatc gagccgacgt tcacgcggaa cgaccaagca agcttggctg ccatttttgg 4560 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc 4620 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca 4680 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga 4740 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg 4800 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa 4860 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca 4920 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt 4980 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct 5040 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc 5100 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg 5160 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 5220 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat 5280 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 5340 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 5400 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 5460 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 5520 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc 5580 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg 5640 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg 5700 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc 5760 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg 5820 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca 5880 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg 5940 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg 6000 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg 6060 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc 6120 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga 6180 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg 6240 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa 6300 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc 6360 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt 6420 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg 6480 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag 6540 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc 6600 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc 6660 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc 6720 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac 6780 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc 6840 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac 6900 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac 6960 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc 7020 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc 7080 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg 7140 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc 7200 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc 7260 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg 7320 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct 7380 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc 7440 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattg 7500 tgattaaggc tcagattcga cggcttggag cggccgacgt gcaggatttc cgcgagatcc 7560 gattgtcggc cctgaagaaa gctccagaga tgttcgggtc cgtttacgag cacgaggaga 7620 aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca 7680 tcgacggcga gatcattggg ctgtcggtct tcaaacagga ggacggcccc aaggacgctc 7740 acaaggcgca tctgtccggc gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg 7800 gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt gatgatcgtc cgacagattc 7860 caacgggaat ctggtggatg cgcatcttca tcctcggcgc acttaatatt tcgctattct 7920 ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg 7980 ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct gctaggtagc ccgatacgat 8040 tgatggcggt cctgggggct atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac 8100 caaacgcagc gctagatcct gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg 8160 cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt gcctctgctc acctttaccg 8220 cctggcaact ggcggccgga ggacttctgc tcgttccagt agctttagtg tttgatccgc 8280 caatcccgat gcctacagga accaatgttc tcggcctggc gtggctcggc ctgatcggag 8340 cgggtttaac ctacttcctt tggttccggg ggatctcgcg actcgaacct acagttgttt 8400 ccttactggg ctttctcagc ccccgagcgc ttagtgggaa tttgtacccc ttatcgaacc 8460 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt 8520 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 8580 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 8640 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 8700 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 8760 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 8820 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 8880 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 8940 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 9000 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 9060 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 9120 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 9180 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca 9240 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg 9300 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt 9360 cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa 9420 ggtggttcta agcctcgtct tgcgatggca tttcgatcca ttcccattcc gcgctcaaga 9480 tggcttcccc tcggcagttc atcagggcta aatcaatcta gccgacttgt ccggtgaaat 9540 gggctgcact ccaacagaaa caatcaaaca aacatacaca gcgacttatt cacacgagct 9600 caaattacaa cggtatatat cctgccagtc agcatcatca caccaaaagt taggcccgaa 9660 tagtttgaaa ttagaaagct cgcaattgag gtctacaggc caaattcgct cttagccgta 9720 caatattact caccggatcc taaccggttt aattaaggcg cgccatgccc gggcaagcgg 9780 ccgcacaagt ttgtacaaaa aagcaggctt ctagacctag gtggagtcat cacgcagact 9840 atctcagcat gtgcgtagca cgcggccgca gcttatagta aagatttttt tgttaataaa 9900 atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt taataaattt 9960 atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt atggatataa 10020 tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt aaaagatatt 10080 ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg cacaccgctt 10140 ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat gctgccacgt 10200 cagctttcac actatgacac cactggccat acacgttgcc acctcagctt tctcctcttc 10260 tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc ccgcctcttc 10320 acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc tataaataga 10380 gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa atagcaaaac 10440 ggatccacaa ccaaaaatgc ttaagctctc ttgtaacgtt accaatcatc ttcacacatt 10500 ctcattcttt agcgattcta gtctgttcat ccctgtcaac agaaggactc ttgctgtgtc 10560 tatggcagcg aggctgcaag agcagaaagg aggtgagatg gacacgagga ttgcgatcat 10620 aggcatgagc gccatacttc cttgtggaac cacggtgaga gagtcatggg agaccataag 10680 ggctggcatt gattgcctgt ctgatcttcc tgaggatcgt gttgatgtga ctgcgtactt 10740 tgacccagtc aagaccacaa aggacaaaat ctactgcaag agaggaggtt tcattcctga 10800 gtatgacttt gatgcgcgtg agtttggtct caacatgttc cagatggaag attctgatgc 10860 gaatcagacc atctcacttc tcaaggtcaa agaagcactt caagatgctg ggattgatgc 10920 acttggaaag gagaagaaaa acataggatg cgtgcttggc attggaggtg gacagaagag 10980 ttcacatgag ttctacagca gactcaacta tgtggttgtg gagaaagtct tgagaaagat 11040 ggggatgcca gaggaagatg tcaaagtggc tgtggagaag tacaaagcga acttcccaga 11100 atggagactt gactctttcc ctggattcct tgggaatgtc actgctggca gatgcaccaa 11160 cacattcaac ttggatggca tgaactgcgt tgtggatgct gcatgtgcaa gctccttgat 11220 agcagtcaag gttgcgattg atgaactgct ctatggtgac tgcgacatga tggtcactgg 11280 tgcaacctgc acagacaact ccattgggat gtacatggct ttctcaaaga cacctgtgtt 11340 cagcacggac ccaagtgtga gagcgtatga tgagaaaacg aaagggatgc tcattggtga 11400 gggatcagcc atgttggtgc tcaaacgtta tgcagatgca gtgagggatg gtgatgagat 11460 tcatgctgtg attcgtggtt gtgcaagctc aagtgatggc aaagcagctg gcatctacac 11520 tcccacaatc agtggtcaag aggaagcctt gagacgtgca tacaatcgtg cctgcgttga 11580 ccctgccact gtcactttgg ttgagggtca tgggactggc actccagttg gagataggat 11640 tgagttgact gcgttgagga acttgtttga caaagcgtat ggtgaaggga acacagagaa 11700 agttgcagtt ggaagtatca agtccagtat tggtcatttg aaggctgttg ctggtcttgc 11760 tggaatgatc aaggtcatca tggctctcaa acacaagact ttgcctggaa cgatcaatgt 11820 ggacaatcca cccaatctct atgacaacac tcccatcaat gagtcatccc tctacatcaa 11880 cacgatgaac agaccctggt tccctccacc tggtgttccg agacgtgctg gaatatccag 11940 ctttggcttt ggaggtgcca actaccatgc agtgttggaa gaggctgaac cagagcacac 12000 gacagcgtac agactcaaca aacgtccaca accggtgttg atgatggctg caactcctgc 12060 tgcgttgcag agcctttgtg aagctcagct gaaagagttt gaagctgcga tcaaggagaa 12120 tgagacagtc aagaatactg cctacatcaa gtgtgtcaag tttggagaac agttcaagtt 12180 ccctgggagc atacctgcga ccaatgcgag actgggattc ttggtcaaag atgcggagga 12240 tgcgtgctcc acgcttcgtg ctatatgtgc tcagtttgcc aaagatgtga ccaaagaagc 12300 gtggaggctt ccgagagaag gagtttcttt ccgtgccaaa ggcattgcca ccaatggtgc 12360 tgttgctgca ctcttctctg gacaaggtgc tcagtacaca cacatgttca gtgaagttgc 12420 gatgaactgg cctcagttca gacagtccat tgctgcaatg gacgctgcac agtccaaggt 12480 tgctggaagt gacaaagact ttgaaagagt ctctcaagtc ctgtacccaa ggaaaccgta 12540 cgaacgtgag ccagaacaag atcacaagaa aatctcactc acagcataca gccagccgtc 12600 aaccctggca tgtgctcttg gtgcctttga gattttcaag gaagctggat tcactccaga 12660 ctttgcagct ggacacagct tgggtgagtt tgctgcgctc tatgcagctg gttgtgttga 12720 cagagatgag ttgtttgaac ttgtttgcag aagggcaagg atcatgggag gcaaagatgc 12780 acctgccaca cccaaaggtt gcatggcagc tgtcatagga cccaacgcag agaacatcaa 12840 ggttcaagct gccaacgtct ggcttggaaa ctcaaactct ccgtctcaga ctgtcattac 12900 tgggtctgtt gaagggattc aagctgaaag tgccagattg cagaaggaag ggttcagagt 12960 tgtgcctctt gcttgtgagt ctgccttcca ttctccacag atggaaaatg ccagctcagc 13020 tttcaaagat gtcatatcca aggtttcctt ccgtacaccc aaagcagaaa caaagctctt 13080 cagcaatgtt tctggagaga cctatccgac ggatgccaga gagatgctca ctcaacacat 13140 gacctcatct gtgaagttcc tgacccaagt gaggaacatg caccaagctg gtgcgagaat 13200 ctttgttgag tttggaccga aacaagttct cagcaaactt gtttctgaaa ccctcaaaga 13260 tgacccgtct gttgtcacag tgtctgtgaa cccagcttct ggcacggact ctgacataca 13320 gttgagggat gctgcagttc agctggttgt ggctggtgtc aatcttcaag ggtttgacaa 13380 gtgggatgct cctgatgcca cgaggatgca agccatcaag aaaaagagaa ccacactcag 13440 actttcagct gccacctatg tcagtgacaa gaccaagaaa gtgagggatg ctgccatgaa 13500 cgacggaaga tgtgtcacat acctcaaggg tgctgcacca ctcatcaagg ctcctgaacc 13560 tgtggttgac gaagctgcca agagggaggc tgaaaggctt cagaaggagc ttcaagacgc 13620 tcagaggcag cttgacgacg ccaaacgtgc agctgcagaa gccaactcca aacttgctgc 13680 tgccaaggag gaagccaaga ctgctgcagc ttcagccaag cctgcagttg acactgctgt 13740 tgtggagaaa catcgtgcca tcctcaagtc catgctggct gagctggatg gttatggaag 13800 tgttgatgct tcttcccttc agcaacagca acagcaacag actgctccgg cgcctgttaa 13860 agccgctgcc cctgccgctc ccgttgcaag tgctcctgct cccgccgtgt ccaacgagtt 13920 gcttgagaaa gctgagacag ttgtgatgga ggttctcgct gccaagactg gatacgaaac 13980 agacatgatt gaggcggaca tggagcttga gactgagttg ggaattgaca gcatcaagag 14040 ggttgaaatt ctttctgaag tccaagctat gcttaacgtt gaggcgaaag atgttgatgc 14100 tctctctagg acgagaacag tgggtgaagt tgtcaacgct atgaaggcag aaatcgctgg 14160 ttcttccgct ccggcgccgg cagctgccgc acctgcaccg gctaaagcgg ctccagctgc 14220 agctgcacca gctgtttcta atgagctgct cgaaaaggca gaaactgttg tgatggaagt 14280 ccttgctgcc aaaactggct acgaaactga catgatagaa tcagacatgg agcttgagac 14340 ggagcttgga attgattcca tcaagcgtgt ggaaatcctc agtgaagtcc aagccatgct 14400 caatgtggaa gctaaggatg tggatgctct ctcaaggacc agaactgtcg gtgaggttgt 14460 caatgccatg aaggctgaga tagctggagg ctccgctcct gcgccggccg ctgccgctcc 14520 tggtcccgct gccgcggctc cagctccagc tgcagccgct ccggccgtct caaacgaact 14580 ccttgaaaaa gctgagaccg tcgtgatgga ggtcttggca gctaaaaccg gctatgagac 14640 tgacatgatt gagtctgaca tggaattgga gacagaactt gggatagaca gcatcaaacg 14700 tgttgaaatc ctcagtgagg tccaagctat gctgaacgtg gaagcgaagg atgttgatgc 14760 tctgtccaga acgaggaccg ttggtgaggt tgtggatgcc atgaaggctg aaattgctgg 14820 agggtctgcg cccgcgccag ctgcagccgc tcctgcaccc gcagccgcgg cacctgctcc 14880 agctgcacct gcacctgccg tttcttcaga actcttggag aaggctgaga cggttgtgat 14940 ggaggttttg gctgcaaaga ctggctatga gaccgacatg attgaatctg atatggaatt 15000 ggagacagag cttgggatcg attccatcaa aagagtggag atcctgagtg aagtccaagc 15060 catgctcaat gttgaagcta aggatgttga tgcactttca agaacaagga cagtgggtga 15120 ggttgtggat gccatgaagg cggagattgc tggagggagc gcgcctgcgc cagctgccgc 15180 ggcacctgca ccggcagccg cagctccagc tccagctgca ccagcaccgg ccgcgcccgc 15240 gccagctgtt tcatccgaac tcctggaaaa ggccgagacg gttgtgatgg aggtgctcgc 15300 agctaagact ggttacgaga ccgacatgat tgaaagtgac atggagcttg aaaccgagtt 15360 gggaatagat agcatcaaac gtgttgaaat cttgtctgag gtccaagcta tgttgaacgt 15420 ggaggcaaaa gatgtcgatg cgctttcaag aaccagaaca gtcggtgagg tcgtggacgc 15480 catgaaggct gaaattgccg gaagctctgc tagtgcgcca gctgcagccg cacctgcacc 15540 tgctgccgcg gcacctgctc cagctgcagc cgctcctgcc gtcagcaatg agttgctgga 15600 gaaagccgaa acagttgtga tggaagtcct tgctgcaaaa actggttacg aaacggacat 15660 gattgagtca gacatggaat tggagaccga acttggaata gattccatca agagagtgga 15720 gatcctctct gaggtgcaag ccatgctcaa cgttgaggct aaagatgttg atgcactcag 15780 taggacaaga actgtggggg aagttgtcga tgcgatgaag gctgagattg ctggaggctc 15840 tgctcctgcg ccggcagctg ccgcacctgc acccgcagcc gcggcaccag ctgtcagcaa 15900 cgaattgctt gagaaggctg aaacagttgt catggaagtg ctggctgcaa aaaccggcta 15960 tgagactgac atgattgagt ctgacatgga acttgagacg gaattgggca ttgacagcat 16020 caagcgtgtt gagatcctca gtgaagtcca agcaatgctc aatgtggagg ctaaggatgt 16080 ggatgccctc tcaaggacca gaacagttgg tgaggtcgtt gatgcgatga aggcggagat 16140 cgctggaagt tccgcgcctg cgccagctgc agccgctcct gcacccgcag ccgcggcacc 16200 ggctccagct gcagccgcac cggccgtctc ctcagaactc ctggaaaagg ctgagacagt 16260 tgtgatggaa gtccttgctg cgaaaactgg ctatgagacc gacatgattg aatctgacat 16320 ggaactggag accgagcttg gaattgactc catcaaacgt gttgaaatcc tctctgaggt 16380 tcaagcgatg ttgaatgtgg aggccaaaga tgttgatgct ctttccagaa caaggacggt 16440 gggagaggtg gttgatgcca tgaaggctga gatagctggg ggttctgctc ctgcgccagc 16500 tgcagccgct cctgctcccg ctgccgcggc accagctgtc agcaacgaac tccttgaaaa 16560 agctgaaact gttgtgatgg aggttttggc tgcaaagact ggatacgaga ctgacatgat 16620 agagtcagac atggagttgg aaacagagct tgggattgat agcatcaagc gtgtggaaat 16680 cctttctgag gttcaagcca tgctgaacgt tgaggccaaa gatgtcgatg ctttgtcaag 16740 gaccagaacg gttggagaag tggtcgatgc catgaaggct gagatagctg gtggatcagc 16800 ccctgctccg gctgcagccg ctcctgcaag tgccggtgct gcaccagctg tgaagattga 16860 ttcagttcat ggtgctgact gtgacgattt gtcactgatg cacgcaaagg tggttgacat 16920 cagaaggcca gatgaactga ttctggaaag accggagaac cgtccagttc ttgtcgttga 16980 cgatggctca gagttgacac ttgctcttgt gagggtgttg ggagcctgtg cagttgtctt 17040 gacctttgag ggattgcagt tggctcagag agctggtgca gctgccattc gtcatgttct 17100 ggctaaggac ctttcagcag aatcagctga gaaggccatc aaagaggctg agcaaagatt 17160 tggagcgttg ggaggcttca taagccagca agcagaaagg tttgaaccag ctgagatact 17220 gggtttcaca ctcatgtgtg cgaagtttgc caaagcctct ctctgcactg ctgtggctgg 17280 aggtcgtcct gccttcatcg gtgtggctag gcttgacggc agacttggat tcacctctca 17340 aggcacttct gacgcactca agagagcaca gcgtggagcg atctttggac tctgcaagac 17400 aatagggttg gagtggtcag aatctgatgt gttcagccgt ggtgtggaca ttgctcaagg 17460 gatgcaccca gaagatgctg cagttgccat agttagggag atggcgtgtg ctgacatcag 17520 aatcagagag gttgggattg gagcaaacca gcaaagatgc acaatcagag ctgccaagtt 17580 ggagactggg aaccctcaga gacagatagc gaaagacgat gtgcttctgg tttctggagg 17640 tgctcgtggg atcacacctc tttgcatccg tgagatcacg cgtcagattg ctggaggcaa 17700 gtacattctg ttgggtcgta gcaaagtttc tgcgtctgaa ccagcatggt gtgctggcat 17760 cactgatgag aaggctgtgc agaaagctgc cacccaagag ttgaagaggg ccttcagtgc 17820 tggtgaagga cccaaaccca ctccaagggc tgtcacgaaa cttgtgggat ctgttcttgg 17880 tgctcgtgaa gtgagaagct ccattgctgc cattgaagct ctgggtggca aagcgatcta 17940 cagctcttgt gatgtgaact ctgctgcaga tgttgccaaa gcagttaggg atgcagagtc 18000 ccagcttggt gccagagtta gtggaatcgt tcacgcttct ggtgtgctcc gtgatcgtct 18060 catagagaag aaactccctg acgagtttga tgctgtcttt gggaccaaag tcactgggtt 18120 ggagaatctt ctggctgcag ttgatcgtgc caacctcaaa cacatggttc tcttcagctc 18180 ccttgctggt ttccacggca atgttggtca gtctgactat gcgatggcaa acgaagctct 18240 caacaagatg ggtttggaac ttgcgaagga tgtcagtgtc aagtccatct gctttggtcc 18300 ctgggatggt gggatggtga cacctcaact caagaaacag tttcaagaaa tgggagttca 18360 gatcattcct cgtgaaggag gtgctgatac agttgcgagg attgtgttgg gatcaagtcc 18420 tgctgagatc cttgttggaa actggaggac accatccaag aaagttggaa gtgacacgat 18480 caccctgcat agaaagatca gtgccaagtc caatcccttc ttggaggatc atgtcataca 18540 aggcagaagg gtgttgccaa tgacccttgc tattgggagc cttgctgaga cctgccttgg 18600 actcttccct ggctactctc tctgggccat agatgacgca cagctcttca aaggtgtcac 18660 tgttgatgga gacgtcaact gtgaagtgac cctcacaccg tccactgctc cgagtgggag 18720 ggtgaatgtc caagcgacgc tcaagacgtt ctcaagtgga aaacttgttc cagcctacag 18780 agctgtcata gtgttgtcca accaaggtgc tccacctgcc aatgcaacca tgcagcctcc 18840 gagccttgat gcagatcctg ctttgcaagg gtcagtctat gatggcaaga ctctctttca 18900 tggtcctgcg ttcagaggca ttgatgacgt tctcagctgc accaagagcc agcttgttgc 18960 caagtgttct gctgttcctg gatcagatgc agccagagga gagtttgcaa ctgacactga 19020 tgcacatgat ccctttgtca atgatcttgc ctttcaagca atgttggtct gggtgaggag 19080 aacgctggga caagctgcac tcccgaactc cattcagagg atagtccaac acagaccggt 19140 cccacaagac aaacccttct acatcaccct cagatccaat cagagtggtg gacatagcca 19200 gcacaaacat gcacttcagt tccacaacga acaaggtgat ctcttcattg atgttcaagc 19260 ctctgtcatt gcgacagact cacttgcgtt ctgagtagtt agcttaatca cttaggtcac 19320 ccagtccaag cccggcctag cacgatgatc atgagtgttg ctattgatgt atgttaatac 19380 tatacacagt catcgtgtgt gttttataaa actgtaagct taggatgttt gaggctaatg 19440 taattagcac tacaccgtaa taaatgagag gtttacttta tgttttaata atgttcttac 19500 ccttgctgct ctctaaactg taatggcgta cggttagcta aactaaagta aaaatcgatt 19560 cgcttgagta aaattagtga ccgggaaaga tcatcgacta ctactgctca gagatgtaat 19620 cggtaattaa gacttcaagc tcaataatta attaggagta aatctggctt cgaattttta 19680 aattgtcaac ttccaaagaa ggattggcaa gactcaatct caattgccca acaaacatat 19740 ggctctttac aaatttggaa tctctttttg atggcaagaa cgtcgtaatt cctctgtcga 19800 tctcatgtaa ttaccaaact catctactta ttactttctt tatatgcact tactgatgcg 19860 gccgcttaat taagatgagt gatactcagg actcaggact cactctgctg atcactagtg 19920 ctagcctcga ggtcgacaca actttgtata caaaagttgt ctagacctag gctcagcaac 19980 tcatccagtc aagttcgagt cagtgagttg ggcggccgca gcttatagta aagatttttt 20040 tgttaataaa atgaaagtta atatttatgg atttatttga acatatttac aagtttttgt 20100 taataaattt atttacagaa gtgttaaatt tgaaaatgtg ttaaatgaga gttaatattt 20160 atggatataa tattacttta tttaaaaata aaatgaattt atattaaaat atgatttggt 20220 aaaagatatt ataaagagga acttggatga gaaagacgac gatgggcaaa agttgcatgg 20280 cacaccgctt ttgttcagta acatctgcac tgtggcgcct ttttcacact caccatgcat 20340 gctgccacgt cagctttcac actatgacac cactggccat acacgttgcc acctcagctt 20400 tctcctcttc tcactatgac acgactggcc atgcatgcat gcatgctgcc acctcagctc 20460 ccgcctcttc acccgtgtct ttctctcatg tcgcagtgct gcgtgccaac ctcttctctc 20520 tataaataga gagaagagaa tgatggttaa actcaaacca aaacaagaaa acatacacaa 20580 atagcaaaac ggatccacaa ccaaaaatgc ttaagctctc ttgtaacgtt accaatcatc 20640 ttcacacatt ctcattcttt agcgattcta gtctgttcat ccctgtcaac agaaggactc 20700 ttgctgtgtc tatggctgca aggaacgtga gtgcggctca tgagatgcat gacgagaaga 20760 ggatagctgt tgtgggcatg gctgttcagt atgctggatg caaaacgaaa gacgagttct 20820 gggaagttct catgaatgga aaagttgaga gcaaagtgat cagcgacaaa cgtcttggtt 20880 caaattaccg tgctgaacac tacaaagctg aaaggagcaa gtatgctgac accttctgca 20940 atgagacata tggaactctt gatgagaacg aaatagacaa tgagcatgaa ctccttttga 21000 accttgccaa acaagccttg gctgaaacat ctgtcaaaga ttcaacaaga tgtggcatag 21060 tttctggatg cctcagcttc ccgatggaca acctccaagg tgaactcttg aatgtgtacc 21120 agaaccatgt tgagaagaaa cttggtgcca gagttttcaa agatgcctct cactggtcag 21180 aaagagaaca gtccaacaaa ccagaggctg gtgacagaag aatcttcatg gaccctgctt 21240 cctttgttgc tgaggaactc aatcttggcg ctctccacta ctctgttgat gctgcctgtg 21300 ccactgcgct ctatgtgctc agactggcgc aagatcactt ggtctctggt gcagctgatg 21360 tgatgctttg tggtgcaaca tgcctgccgg agccgttttt catactctct gggttctcaa 21420 ccttccaagc catgcctgtt ggaacgggtc agaatgtgtc catgccgctg cacaaagatt 21480 ctcaaggatt gacccctggt gaaggaggtt ccatcatggt cctcaaacgt ttggatgacg 21540 caatcagaga tggagatcac atctatggaa cacttttggg agccaatgtc agcaacagtg 21600 gcactggtct gccacttaaa ccccttttgc cgtcagagaa aaagtgcctc atggacacct 21660 acacacgtat caatgtgcat ccccacaaga ttcagtatgt tgaatgccat gcaactggga 21720 ctccacaagg tgatcgtgtg gaaattgatg ctgtgaaggc ttgctttgaa ggcaaagtcc 21780 cacgttttgg aaccacaaaa gggaactttg gacacacatt ggtggcagct gggtttgctg 21840 gcatgtgcaa agtgcttctc tccatgaagc acgggatcat tccacccact ccgggaatag 21900 atgacgaaac gaagatggac cctctggttg tctctggtga ggccattccc tggccagaaa 21960 cgaatggaga accgaagcgt gctggtctct cagcgtttgg atttggtggg accaatgctc 22020 atgctgtctt tgaggaacat gacccaagca atgctgcatg cactggccat gactccattt 22080 ctgctctctc tgcacgttgt ggtggtgagt caaacatgag aatagcaatc actgggatgg 22140 atgccacttt tggtgctttg aaaggattgg atgcttttga aagggccatc tacactggtg 22200 ctcatggtgc tataccactc ccagaaaaga gatggagatt cctggggaaa gacaaggact 22260 tccttgatct ttgtggagtc aaagcaacac cgcatggctg ttacattgaa gatgttgaag 22320 ttgatttcca gaggctgagg actccaatga ctcctgaaga tatgctcttg cctcaacagc 22380 ttctggctgt caccacaatt gatagggcca tattggactc tggcatgaag aaaggtggaa 22440 atgtggctgt ctttgtgggg ttggggaccg atcttgaact ctaccgtcac cgtgcaaggg 22500 ttgctctcaa ggagagagtg aggcctgaag ccagcaagaa actcaatgac atgatgcagt 22560 acatcaatga ttgtgggaca agcacttctt acaccagcta cataggcaac ttggttgcaa 22620 caagggtcag ttcacaatgg ggcttcacgg gaccttcctt cacaatcaca gaaggcaaca 22680 attctgtgta cagatgtgct gaacttggca aatacctctt ggaaacggga gaggttgatg 22740 gggttgtggt tgcgggtgtt gatctctgtg ggagtgctga gaacctttac gtcaagtcaa 22800 ggcgtttcaa ggtgtctaca tctgacaccc cgagagccag ctttgatgca gctgctgatg 22860 gttactttgt tggagagggc tgtggtgcat ttgtgctcaa gcgtgagaca agctgcacca 22920 aggatgacag aatctatgct tgcatggatg ccatagttcc tggaaatgtt ccatctgcat 22980 gtttgagaga ggcattggat caagccagag tcaagcctgg agacatcgag atgttggagc 23040 tttctgctga ttcagcgagg catttgaagg acccgtctgt tctcccaaag gaactcactg 23100 ctgaggaaga gattggcgga cttcagacga ttcttcgtga cgatgacaag ctcccaagga 23160 atgttgcaac tggaagtgtc aaggccactg ttggtgacac tggttatgct tctggtgctg 23220 ccagtttgat taaggctgcg ctttgcatct acaaccgtta cctgcccagc aatggagatg 23280 actgggatga acctgcacct gaagcaccct gggacagcac actctttgcg tgccagacct 23340 caagagcttg gttgaagaac cctggtgaga gacgttatgc ggctgtttcc ggagtcagtg 23400 aaaccagaag ttgctacagt gtgctgcttt ctgaagcaga gggacactac gagagagaga 23460 acagaatcag tttggatgag gaagcaccca agctcattgt gttgagagca gactcccatg 23520 aagagattct tggaagattg gacaagataa gggaaaggtt cttgcagccc acgggtgctg 23580 cacccagaga gtctgaattg aaggcgcaag cccgtaggat ctttcttgaa ctccttggtg 23640 agacccttgc ccaagatgca gcttcatctg gaagtcagaa gccccttgct ctcagcttgg 23700 tctccactcc atccaagctc cagagagagg tggaacttgc ggcaaaaggc attccgagat 23760 gtctcaagat gagacgtgat tggagttccc ctgctggaag cagatatgcg cctgagcctt 23820 tggccagtga cagagttgcc ttcatgtatg gagaaggaag gagcccttac tatggcatca 23880 cacaagacat tcataggatt tggcctgaac ttcatgaggt catcaatgag aaaacaaacc 23940 gtctctgggc tgaaggtgac agatgggtca tgccaagggc cagcttcaag agtgagcttg 24000 agtcacaaca gcaagagttt gacagaaaca tgattgaaat gttccgtctt ggaatcctca 24060 cctcaattgc cttcacaaac ctggcgagag atgttctcaa catcacgccc aaagcagcct 24120 ttggactcag tttgggagag atttccatga tctttgcttt ctccaagaaa aacggtttga 24180 taagcgatca gttgacaaag gatttgagag agtcagatgt ctggaacaaa gctctggctg 24240 ttgagttcaa tgcgctcaga gaagcctggg gcattccaca gtctgtccca aaagatgagt 24300 tctggcaagg ttacatagtg aggggaacga aacaagacat agaagcagcc atagcaccag 24360 actcaaagta tgtgaggttg accatcatca atgatgccaa cactgctctc atcagtggaa 24420 agcctgatgc atgcaaagct gcgattgctc gtcttggtgg caacatccct gcgcttccag 24480 tcactcaagg catgtgtggg cattgcccag aggtgggacc ttacaccaag gacatcgcaa 24540 agatccacgc caaccttgag ttccctgtgg ttgatggtct tgatctctgg actaccataa 24600 accagaagag gcttgtgcca agagccacgg gtgccaaaga tgaatgggct ccttcatcct 24660 ttggggagta tgctggacag ctctatgaga aacaagccaa cttccctcag atagttgaaa 24720 ccatctacaa gcagaactat gatgtgtttg ttgaagttgg gccaaacaat cacagaagca 24780 cagcagtgag aaccacgctt ggaccacaga gaaaccacct tgctggtgcc atagacaagc 24840 agaacgagga tgcttggacg acaatagtca agctcgtggc ttcattgaag gctcaccttg 24900 ttcctggagt gaccatcagt ccgctctacc actccaagct cgttgcagag gctgaggctt 24960 gttatgctgc gctctgtaaa ggtgagaaac ccaagaaaaa caagtttgtg agaaagattc 25020 agttgaatgg cagattcaac agcaaggcag accccatctc ctcagcagac cttgcatcct 25080 tcccacctgc ggaccctgcg attgaagctg ccatctcttc aaggatcatg aagcctgtgg 25140 ctccgaaatt ctatgcgcgt ctcaacattg atgagcaaga tgagaccaga gatccgatct 25200 tgaacaagga caatgcgcct tccagctcca gctcttccag cagttcctct tccagcagtt 25260 catctccgtc tcctgctcct tcagctccag ttcagaagaa agctgcccca gctgcggaga 25320 caaaagctgt tgccagtgct gatgcacttc gtagtgccct gcttgatctt gactccatgc 25380 ttgcgctcag ctctgcaagt gcttctggca accttgttga gactgcgcca tctgatgcca 25440 gtgtcattgt ccctccgtgc aacattgctg atcttggcag cagagctttc atgaaaactt 25500 atggtgtttc tgcgcctctg tacaccggtg caatggccaa gggaattgcc tctgcggact 25560 tggtgatcgc agctggaagg caaggaatcc ttgcgtcatt tggtgctgga ggccttccaa 25620 tgcaagttgt gcgtgagtcc attgaaaaga ttcaagctgc cctgcccaat ggaccatatg 25680 ctgtcaacct catccattct ccttttgaca gcaaccttga gaagggaaat gttgatctct 25740 tcttggaaaa gggtgtcact tttgttgagg cttcagcctt catgacgttg acaccgcaag 25800 ttgtcagata cagagcggct gggttgacga ggaatgcaga tgggtcagtc aacataagga 25860 acagaatcat tgggaaagtc tccagaactg agctggctga aatgttcatg cgtcctgcac 25920 ctgagcatct tttgcagaag ctcattgctt ctggggagat caatcaagaa caagctgagt 25980 tggccagacg tgttcctgtg gctgatgaca ttgcggttga agctgattct ggtggacaca 26040 ctgacaaccg tccaatccat gtcattctgc ctctgattat caacctgagg gataggcttc 26100 acagagagtg tggataccca gcaaacctga gggttcgtgt tggtgctggg ggtggcattg 26160 ggtgtcccca agctgcgctg gcaactttca acatgggtgc ctctttcatt gtcactggaa 26220 cagtgaacca agttgccaaa caatctggga cgtgtgacaa tgtgaggaaa cagcttgcga 26280 aagcaactta ctcagatgtt tgcatggcac cggcagctga catgtttgag gaaggtgtga 26340 aacttcaagt tttgaagaaa ggaacaatgt tcccttcaag agccaacaaa ctctatgagt 26400 tgttctgcaa gtatgattct tttgagtcca tgcctccagc agagcttgcg agggttgaga 26460 aaagaatctt cagtcgtgca ttggaagagg tctgggatga gaccaagaac ttctacatca 26520 accgtctcca caacccagag aagatacaga gggctgagag ggaccccaaa ctgaagatgt 26580 cactgtgttt cagatggtat ctgtccctgg cttcaaggtg ggccaacact ggagcttctg 26640 acagagtcat ggactaccaa gtttggtgtg gtcctgccat tggttcattc aatgatttca 26700 tcaaaggaac ttaccttgat ccggcagtgg caaatgagta cccgtgtgtt gtgcagatca 26760 acaaacagat tcttagagga gcgtgcttct tgaggcgtct tgaaatactg agaaatgcaa 26820 ggctttcaga tggtgcagct gcacttgtgg cgagcattga tgacacatat gtccctgctg 26880 agaagctgtg agtagttagc ttaatcactt aggtcaccca gtccaagccc ggcctagcac 26940 gatgatcatg agtgttgcta ttgatgtatg ttaatactat acacagtcat cgtgtgtgtt 27000 ttataaaact gtaagcttag gatgtttgag gctaatgtaa ttagcactac accgtaataa 27060 atgagaggtt tactttatgt tttaataatg ttcttaccct tgctgctctc taaactgtaa 27120 tggcgtacgg ttagctaaac taaagtaaaa atcgattcgc ttgagtaaaa ttagtgaccg 27180 ggaaagatca tcgactacta ctgctcagag atgtaatcgg taattaagac ttcaagctca 27240 ataattaatt aggagtaaat ctggcttcga atttttaaat tgtcaacttc caaagaagga 27300 ttggcaagac tcaatctcaa ttgcccaaca aacatatggc tctttacaaa tttggaatct 27360 ctttttgatg gcaagaacgt cgtaattcct ctgtcgatct catgtaatta ccaaactcat 27420 ctacttatta ctttctttat atgcacttac tgatgcggcc gcttaattaa tctgacctga 27480 ccactagacc tgagtgagta cagatgtgct actagtgcta gcctcgaggt cgaccaactt 27540 ttctatacaa agttgtctag acctaggact aggcactgag tgactcactg acctgactga 27600 gtgactggcg gccgcagctt atagtaaaga tttttttgtt aataaaatga aagttaatat 27660 ttatggattt atttgaacat atttacaagt ttttgttaat aaatttattt acagaagtgt 27720 taaatttgaa aatgtgttaa atgagagtta atatttatgg atataatatt actttattta 27780 aaaataaaat gaatttatat taaaatatga tttggtaaaa gatattataa agaggaactt 27840 ggatgagaaa gacgacgatg ggcaaaagtt gcatggcaca ccgcttttgt tcagtaacat 27900 ctgcactgtg gcgccttttt cacactcacc atgcatgctg ccacgtcagc tttcacacta 27960 tgacaccact ggccatacac gttgccacct cagctttctc ctcttctcac tatgacacga 28020 ctggccatgc atgcatgcat gctgccacct cagctcccgc ctcttcaccc gtgtctttct 28080 ctcatgtcgc agtgctgcgt gccaacctct tctctctata aatagagaga agagaatgat 28140 ggttaaactc aaaccaaaac aagaaaacat acacaaatag caaaacggat ccacaaccaa 28200 aaatgcttaa gctctcttgt aacgttacca atcatcttca cacattctca ttctttagcg 28260 attctagtct gttcatccct gtcaacagaa ggactcttgc tgtgtctatg gcgcttcgtg 28320 tcaagaccaa caagaagcca tgttgggaga tgaccaagga ggaactgacc tctggcaaaa 28380 ctgaggtgtt caactatgag gaactcctgg agtttgcaga gggtgacata gctaaggttt 28440 ttggtccgga gtttgctgtc atagacaagt accccagaag ggtgaggctg cctgctcgtg 28500 aatacctgct tgtgacaagg gtcacactca tggatgccga ggtcaacaac tacagagttg 28560 gtgccagaat ggtcactgag tatgatcttc cagtgaatgg cgaattgtct gagggaggtg 28620 attgtccctg ggcagttctg gttgagagtg gacagtgcga tttgatgctg atatcataca 28680 tgggaattga tttccagaac caaggtgaca gagtttaccg tctgctcaac accacgttga 28740 cattctatgg ggttgcccat gagggagaga cccttgagta tgacatcaga gtgaccggtt 28800 ttgccaaacg tttggatggt ggcatctcca tgttcttctt tgagtatgac tgctatgtca 28860 atgggaggct tctcattgag atgagagatg gctgtgctgg cttcttcaca aatgaggagc 28920 ttgatgcggg aaagggtgtg gtgttcacaa gaggggattt ggctgccaga gctaagattc 28980 caaagcaaga tgtttctccc tatgctgtcg ctccctgcct tcacaaaaca aaactcaacg 29040 aaaaggagat gcagaccttg gttgacaaag attgggcttc cgtttttggc tcaaagaatg 29100 gcatgccgga gatcaactac aaactctgcg cacgtaagat gctcatgatt gatagagtca 29160 ccagcattga ccataaggga ggtgtgtacg gtttgggtca gttggttggt gagaaaatct 29220 tggagaggga ccattggtac ttcccatgcc attttgttaa ggatcaagtc atggctggat 29280 ctcttgtcag cgacggttgt tcacaaatgc tcaagatgta catgatctgg ctcggtttgc 29340 accttaccac cggaccattt gacttcagac ccgtcaacgg acacccgaac aaagttaggt 29400 gtaggggtca gatatctcct cataagggaa agttggtcta cgtcatggag atcaaagaga 29460 tgggattcga cgaggataac gatccgtacg ccattgctga cgtcaacatc attgacgttg 29520 atttcgaaaa gggtcaagat ttcagccttg atagaatcag cgactacgga aagggggatc 29580 ttaacaaaaa gatcgtcgtg gacttcaagg gcattgctct caagatgcag aagagatcca 29640 cgaacaagaa cccctccaaa gttcagcccg tttttgctaa cggtgcagcc actgtgggac 29700 cggaagcgtc caaggccagc tctggtgcca gtgctagtgc ttctgctgcc cctgcgaagc 29760 ctgctttctc agcagatgtt cttgcgccaa agccagttgc acttcctgaa catatcctca 29820 agggtgacgc tctcgctccg aaagaaatgt cctggcatcc catgtcaaaa cttcctggca 29880 acccgacgcc gttcttctcc cctagttctt accctccgag ggcaatttgc ttcatccctt 29940 tccctggcaa cccacttgac aacaattgca aagctggaga aatgcctctg aactggtaca 30000 acatgtcaga gttcatgtgt ggaaaagtta gcaactgctt gggaccagag ttcgcaagat 30060 ttgataagtc aaatacgagc agaagccctg cttttgattt ggctcttgtg actagagttg 30120 ttgaagtcac aaacatggaa catggcaagt tcctgaatgt tgattgcaat cccagcaaag 30180 gcacaatggt gggggagttt gactgtcctc aagatgcgtg gttctttgat ggttcttgca 30240 atgatgggca catgccttat tccatcatca tggaaatcgg acttcagaca tccggtgttc 30300 tcaccagtgt gttgaaagca ccactgacga tggacaaaga tgacattctc tttaggaacc 30360 ttgatgcaag tgctgaaatg gtgaggccag atgtggatgt gagggggaaa accataagga 30420 atgtgaccaa gtgcactgga tatgcaatgt tgggaaagat ggggattcac agattcacgt 30480 ttgagttgtc tgttgatggc gtggttttct acaaaggaag cacttccttt ggatggttca 30540 ctccagaggt gtttgctcag caagctggac ttgacaatgg gaaaaagacg gagccttggt 30600 gcaaaactaa caacacgagt gtgagaagag ttgaaatcgc atcagccaaa ggcaaagaac 30660 agctgactga gaaacttcca gatgcaacta atgctcaagt tctcagaagg tcagaacagt 30720 gtgagtattt ggattacctc aacattgctc ctgattctgg gctgcatggg aaaggatatg 30780 cgcatggaca caaagatgtt aacccacaag actggttctt ctcttgtcac ttttggtttg 30840 atcctgtgat gcctggatct ttgggaattg agtcaatgtt ccagctcatt gaggcatttg 30900 cggtggacca gaacataccg ggagaataca atgtgagcaa tccgaccttt gcccatgccc 30960 ctggaaaaac ggcgtggaag taccgtggtc aattgacacc aaagaaccgt gcgatggact 31020 gtgaggtgca catagtttca atcacagcct ctcctgaaaa tggaggctac gttgacatag 31080 tggcagatgg agcactttgg gttgatggtc tccgtgtgta tgaagccaaa gaactcagag 31140 ttcgtgttgt cagtgcaaaa cctcaagcca ttccggatgt tcaacaacag ccacccagcg 31200 caaaagcgga ccctgggaaa actggagttg cactcagtcc gactcagttg agagatgtcc 31260 tgcttgaagt ggacaatcca ttgtatcttg gtgttgaaaa ctccaatttg gtgcagtttg 31320 aatccaaacc tgcaacttct tcaaggattg tcagtatcaa accatgctcc ataagtgatc 31380 ttggtgacaa gtccttcatg gaaacgtaca atgtgtcagc acctctgtac actggagcaa 31440 tggccaaggg aattgcgtct gcggaccttg tcattgctgc tgggaagagg aagatattgg 31500 gttcttttgg agcgggtggg cttccaatgc accatgtgag ggcagctttg gagaagatac 31560 aagcggctct gcctcaagga ccatatgctg tgaacctcat tcattcacct tttgacagca 31620 acttggaaaa ggggaatgtt gatctcttcc tcgaaaaggg cgttactgtg gtggaggctt 31680 ctgcattcat gaccctcact ccccaagttg ttcgttaccg tgcagccgga ctctcaagga 31740 acgctgatgg gagtgtcaac atcagaaacc gtatcattgg aaaagtgtcc agaacagaac 31800 tcgccgaaat gttcatcaga ccggctccgg aacacctcct tgagaaactc atagcttctg 31860 gtgagataac acaagaacaa gccgaacttg cccgtcgtgt tcctgtcgca gacgacatcg 31920 ctgttgaagc tgattctggt ggtcacactg acaaccgtcc catccacgtc atcttgcctt 31980 tgatcatcaa ccttaggaac agactgcaca gagagtgtgg ttatcctgcg caccttagag 32040 tcagagttgg agctggaggc ggtgttggct gcccacaagc tgctgcggct gccctcacaa 32100 tgggagctgc cttcatagtg actgggactg tcaatcaagt tgctaaacag tccgggacat 32160 gtgacaacgt gagaaaacaa ctcagtcaag ccacctacag tgacatttgc atggctccag 32220 cagctgatat gtttgaggaa ggtgtgaaac tccaagttct caagaaggga acaatgttcc 32280 catcaagagc taacaaattg tacgaactct tttgcaagta cgactctttc gattcaatgc 32340 ctcctgctga acttgaaagg atcgagaaga ggatcttcaa gagggcactt caagaggtct 32400 gggaagagac caaggacttt tacatcaatg gtttgaagaa tccagagaag atacagagag 32460 cggaacatga ccccaaactc aagatgtctt tgtgtttcag atggtatctt ggtcttgcat 32520 cacgttgggc caacatgggt gctcctgatc gtgtcatgga ctatcaagtc tggtgtggtc 32580 cagccattgg agccttcaat gacttcatca aaggaacata tcttgatcct gctgtctcta 32640 atgagtaccc atgtgttgtc cagatcaacc ttcagatttt gcgtggtgca tgttacctga 32700 ggcgtcttaa tgctctgaga aatgatccga ggattgatct tgagactgaa gatgctgcct 32760 ttgtctatga gccaacgaat gcgctttgag tagttagctt aatcacttag gtcacccagt 32820 ccaagcccgg cctagcacga tgatcatgag tgttgctatt gatgtatgtt aatactatac 32880 acagtcatcg tgtgtgtttt ataaaactgt aagcttagga tgtttgaggc taatgtaatt 32940 agcactacac cgtaataaat gagaggttta ctttatgttt taataatgtt cttacccttg 33000 ctgctctcta aactgtaatg gcgtacggtt agctaaacta aagtaaaaat cgattcgctt 33060 gagtaaaatt agtgaccggg aaagatcatc gactactact gctcagagat gtaatcggta 33120 attaagactt caagctcaat aattaattag gagtaaatct ggcttcgaat ttttaaattg 33180 tcaacttcca aagaaggatt ggcaagactc aatctcaatt gcccaacaaa catatggctc 33240 tttacaaatt tggaatctct ttttgatggc aagaacgtcg taattcctct gtcgatctca 33300 tgtaattacc aaactcatct acttattact ttctttatat gcacttactg atgcggccgc 33360 ttaattaatc acgagtggat gactgtcacg actgactcat gactgactac tagtgctagc 33420 ctcgaggtcg acacaacttt gtataataaa gttgtctaga cctaggcgag tcatgagtcg 33480 tgacatgacg tcatgctcat cagactgcgg ccgcagctta tagtaaagat ttttttgtta 33540 ataaaatgaa agttaatatt tatggattta tttgaacata tttacaagtt tttgttaata 33600 aatttattta cagaagtgtt aaatttgaaa atgtgttaaa tgagagttaa tatttatgga 33660 tataatatta ctttatttaa aaataaaatg aatttatatt aaaatatgat ttggtaaaag 33720 atattataaa gaggaacttg gatgagaaag acgacgatgg gcaaaagttg catggcacac 33780 cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc acactcacca tgcatgctgc 33840 cacgtcagct ttcacactat gacaccactg gccatacacg ttgccacctc agctttctcc 33900 tcttctcact atgacacgac tggccatgca tgcatgcatg ctgccacctc agctcccgcc 33960 tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg ccaacctctt ctctctataa 34020 atagagagaa gagaatgatg gttaaactca aaccaaaaca agaaaacata cacaaatagc 34080 aaaacggatc cacaaccaaa aatgcccact ccagatgcca ctgcgcctct caacaagccg 34140 tcagactatg ctgtgtacca cgaagaggat ggtcctttct ggactgctga ttcctctggt 34200 gtcagcagag tgaacttctc tgaaactggt gtgggatctg aaggtgtcat tcctgcgctc 34260 acactgattg atgtgtttga gcgtgcggtc aaacgtggtg gcaataggat tgcattcaga 34320 acagagaaca tgccaacctt gaggcgtgga gaagaggctc ctgatgcttt gccgctcaaa 34380 gattggaagt cctggtcttg gaaacagtac aaagctgatg ttcacagaat tgcgagggct 34440 ctgatggatt tgggagttga gcagcacgat gcggtctcca tctttggctt caactcacca 34500 gaatggttcc tcagtgctgt cggtgcagtt catgctggtg ccaagatcgc tggaatctac 34560 ccttcagaca ctcctgctca agtgcagtac aaagccttcc actctgacac tgctgttgca 34620 gttgtggaga acgaacagtg cttcaagaaa ttcgcagagg tggttgagga tctcccttac 34680 ctcaaagcca ttgtttgttg ggattatgaa gccacagaca tcacaaggga agatggttca 34740 gtggttgaag tcctgacctt tgctgagttc ctgaagagag gtgacactgt ggaagcagct 34800 gcacttgatg agagaatcag caagattgag cccaccatgt gtgctgccct gatctacacc 34860 agtggcacaa ctgggagacc caaagctgtc atgatctccc atgacaacct tgtctttgaa 34920 gcatctgctg tggtcccgaa cttgggaggg gcatgcacga ccactgctga ggaaaggatt 34980 ctttcctacc ttcctttgtc tcatgttgct ggaatgatgg ttgacatcat tgctccaatc 35040 atagcgacag cgttccacaa aggaaggatc tgtgtttgtt ttgccagacc gtatgatttg 35100 agaactggaa cccttgggca aagactcaat gcagtggagc caaccatatt ccttggtgtg 35160 ccacgtgtgt gggaaaagat ccaagagaaa ttgatggctg ttggtgccaa aaccactggt 35220 ctcaagaaga aactcagcac ggcagccaag aaacgtgggc ttgaatttca agaggaacaa 35280 cagataggga gaagtggtgc caaccctggc tttggaccgc ttggaatcta caagaaactt 35340 ttgggcctca tcaaaggaaa gttggggctg accaaatgca agtttgcctt tgctggcgca 35400 gctccaatga cccgtgaaac ccttcagtac tttggagcgc tgaacatcaa catcaatgag 35460 gtctatggaa tgtctgaatg cagtggggct gccacctggt caactgacaa agctcatgag 35520 tggggcactg ttggctatga gatgccctcc tgtgaagtca gagtgttcaa gattgcggag 35580 gatggcacca agacagagtg cccaagagct gctgacatca tgcatgccac agaagaggaa 35640 caaggtgaag tttgcttccg tggaaggaac atcatgatgg gatacttggc caacccgaaa 35700 cttggtgatg accatgttgc tgagattgaa gagaagaatg cagctgccat agatagcgaa 35760 gggtggcttc actctggtga caagggagca atttccacaa ggggaatgct caagataacg 35820 ggcagataca aggagcttat cataggtgct ggtggggaga atgtggctcc tgttcccatt 35880 gaagatgcca tcaaggcgag gatgcctttt gtttcaaatg caatgatggt tggagacaag 35940 aggaagttca tggctgtcct tctgactttg aaaacggttg gagcaactgg agagcttcct 36000 ggcacaaaca agctcatggg agcagctgcg gactatggtg agaccattga ggatgcctgt 36060 gacaatgagg cgctcattga agagataacg cagcaactca aagagatagg tgacgatgga 36120 gatgtcacgc caagcaatgc agcccgtata caaaagttca cgattttgcc actcgacttc 36180 agtgtctcca cagatgaact gacagcaacg ttgaaattga agagaagtgt ggttgcagac 36240 aagtatgagg acatcataga ggctttctat gagagcaagt ctgtctttgt gccttactca 36300 acagttggag cttatgcaac gggtggacca gttgatgact ctgttgtgga tggatcattc 36360 aaaggtgact ttagcatgat tggggatgac gatccggatc ttcagaatgt ggatgtcctt 36420 gaagcaattg atgaggacaa ctgagtagtt agcttaatca cttaggtcac ccagtccaag 36480 cccggcctag cacgatgatc atgagtgttg ctattgatgt atgttaatac tatacacagt 36540 catcgtgtgt gttttataaa actgtaagct taggatgttt gaggctaatg taattagcac 36600 tacaccgtaa taaatgagag gtttacttta tgttttaata atgttcttac ccttgctgct 36660 ctctaaactg taatggcgta cggttagcta aactaaagta aaaatcgatt cgcttgagta 36720 aaattagtga ccgggaaaga tcatcgacta ctactgctca gagatgtaat cggtaattaa 36780 gacttcaagc tcaataatta attaggagta aatctggctt cgaattttta aattgtcaac 36840 ttccaaagaa ggattggcaa gactcaatct caattgccca acaaacatat ggctctttac 36900 aaatttggaa tctctttttg atggcaagaa cgtcgtaatt cctctgtcga tctcatgtaa 36960 ttaccaaact catctactta ttactttctt tatatgcact tactgatgcg gccgcttaat 37020 taatctgatc atggatgcta cgtcacggca gtacaggact atcactagac ctaggagctg 37080 tgctgggtaa ctcacatgag tcactggtca catcagcggc cgcagcttat agtaaagatt 37140 tttttgttaa taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt 37200 ttgttaataa atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat 37260 atttatggat ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt 37320 tggtaaaaga tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc 37380 atggcacacc gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat 37440 gcatgctgcc acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca 37500 gctttctcct cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca 37560 gctcccgcct cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc 37620 tctctataaa tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac 37680 acaaatagca aaacggatcc acaaccaaaa atgcttaagc tctcttgtaa cgttaccaat 37740 catcttcaca cattctcatt ctttagcgat tctagtctgt tcatccctgt caacagaagg 37800 actcttgctg tgtctatgct tcagcacact tggcttccga agcctcccaa tctgaccctc 37860 ttgtcagatg aggttcatct ctggaggatt cctcttgacc agcctgagtc acaacttcaa 37920 gaccttgctg ccaccctgag cagtgatgaa ttggcgaggg caaacagatt ctactttcca 37980 gaacacagaa ggcgtttcac tgctgggaga ggcatcctca gatccatctt gggtggatac 38040 ttgggagtgg aaccgggtca agtcaagttt gattatgagt cccgtgggaa accgatcctt 38100 ggtgacagat ttgctgagag tggactcctg ttcaacttgt ctcacagcca gaaccttgcc 38160 ttgtgtgctg tcaactacac gcgtcaaata ggcattgatc ttgaatatct gcgtccaaca 38220 tctgacttgg agtctcttgc aaagaggttc tttctcccaa gagaatatga actcttgagg 38280 tcactccctg atgagcagaa acagaagatt ttctttcgtt actggacttg caaagaggct 38340 tatctcaaag caacgggaga tggaatagcc aaacttgaag agatcgagat agcactcacc 38400 ccaacagaac ctgccaagct ccaaacagct cctgcgtggt ctctgttgga gcttgtgcca 38460 gatgacaatt gtgttgcagc tgtggctgtt gcgggttttg gttggcagcc caagttctgg 38520 cattactgag tagttagctt aatcacttag gtcacccagt ccaagcccgg cctagcacga 38580 tgatcatgag tgttgctatt gatgtatgtt aatactatac acagtcatcg tgtgtgtttt 38640 ataaaactgt aagcttagga tgtttgaggc taatgtaatt agcactacac cgtaataaat 38700 gagaggttta ctttatgttt taataatgtt cttacccttg ctgctctcta aactgtaatg 38760 gcgtacggtt agctaaacta aagtaaaaat cgattcgctt gagtaaaatt agtgaccggg 38820 aaagatcatc gactactact gctcagagat gtaatcggta attaagactt caagctcaat 38880 aattaattag gagtaaatct ggcttcgaat ttttaaattg tcaacttcca aagaaggatt 38940 ggcaagactc aatctcaatt gcccaacaaa catatggctc tttacaaatt tggaatctct 39000 ttttgatggc aagaacgtcg taattcctct gtcgatctca tgtaattacc aaactcatct 39060 acttattact ttctttatat gcacttactg atgcggccgc ttaattaact actgtcactg 39120 aggccgtaga cgagtacgga ctgatctaac tagtgctagc ctcgaggtcg acacccagct 39180 ttc 39183 <210> 44 <211> 38742 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB101476 <400> 44 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga 900 ggattgcgat cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat 960 gggagaccat aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg 1020 tgactgcgta ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag 1080 gtttcattcc tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg 1140 aagattctga tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg 1200 ctgggattga tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag 1260 gtggacagaa gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag 1320 tcttgagaaa gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag 1380 cgaacttccc agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg 1440 gcagatgcac caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg 1500 caagctcctt gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca 1560 tgatggtcac tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa 1620 agacacctgt gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga 1680 tgctcattgg tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg 1740 atggtgatga gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag 1800 ctggcatcta cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc 1860 gtgcctgcgt tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag 1920 ttggagatag gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag 1980 ggaacacaga gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg 2040 ttgctggtct tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg 2100 gaacgatcaa tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat 2160 ccctctacat caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg 2220 ctggaatatc cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg 2280 aaccagagca cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg 2340 ctgcaactcc tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg 2400 cgatcaagga gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag 2460 aacagttcaa gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca 2520 aagatgcgga ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg 2580 tgaccaaaga agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg 2640 ccaccaatgg tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt 2700 tcagtgaagt tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg 2760 cacagtccaa ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc 2820 caaggaaacc gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat 2880 acagccagcc gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg 2940 gattcactcc agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag 3000 ctggttgtgt tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg 3060 gaggcaaaga tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg 3120 cagagaacat caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc 3180 agactgtcat tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg 3240 aagggttcag agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa 3300 atgccagctc agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag 3360 aaacaaagct cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc 3420 tcactcaaca catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag 3480 ctggtgcgag aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg 3540 aaaccctcaa agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg 3600 actctgacat acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc 3660 aagggtttga caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga 3720 gaaccacact cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg 3780 atgctgccat gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca 3840 aggctcctga acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg 3900 agcttcaaga cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact 3960 ccaaacttgc tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag 4020 ttgacactgc tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg 4080 atggttatgg aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc 4140 cggcgcctgt taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg 4200 tgtccaacga gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga 4260 ctggatacga aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg 4320 acagcatcaa gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga 4380 aagatgttga tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg 4440 cagaaatcgc tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag 4500 cggctccagc tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg 4560 ttgtgatgga agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca 4620 tggagcttga gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag 4680 tccaagccat gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg 4740 tcggtgaggt tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg 4800 ccgctgccgc tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg 4860 tctcaaacga actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa 4920 ccggctatga gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag 4980 acagcatcaa acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga 5040 aggatgttga tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg 5100 ctgaaattgc tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg 5160 cggcacctgc tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg 5220 agacggttgt gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat 5280 ctgatatgga attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga 5340 gtgaagtcca agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa 5400 ggacagtggg tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg 5460 cgccagctgc cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac 5520 cggccgcgcc cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga 5580 tggaggtgct cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc 5640 ttgaaaccga gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag 5700 ctatgttgaa cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg 5760 aggtcgtgga cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag 5820 ccgcacctgc acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca 5880 atgagttgct ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt 5940 acgaaacgga catgattgag tcagacatgg aattggagac cgaacttgga atagattcca 6000 tcaagagagt ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg 6060 ttgatgcact cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga 6120 ttgctggagg ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac 6180 cagctgtcag caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg 6240 caaaaaccgg ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg 6300 gcattgacag catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg 6360 aggctaagga tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga 6420 tgaaggcgga gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg 6480 cagccgcggc accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa 6540 aggctgagac agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga 6600 ttgaatctga catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa 6660 tcctctctga ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca 6720 gaacaaggac ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg 6780 ctcctgcgcc agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg 6840 aactccttga aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg 6900 agactgacat gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca 6960 agcgtgtgga aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg 7020 atgctttgtc aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag 7080 ctggtggatc agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag 7140 ctgtgaagat tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa 7200 aggtggttga catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag 7260 ttcttgtcgt tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct 7320 gtgcagttgt cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca 7380 ttcgtcatgt tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg 7440 ctgagcaaag atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac 7500 cagctgagat actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca 7560 ctgctgtggc tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg 7620 gattcacctc tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg 7680 gactctgcaa gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg 7740 acattgctca agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt 7800 gtgctgacat cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca 7860 gagctgccaa gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc 7920 tggtttctgg aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga 7980 ttgctggagg caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat 8040 ggtgtgctgg catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga 8100 gggccttcag tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg 8160 gatctgttct tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg 8220 gcaaagcgat ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta 8280 gggatgcaga gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc 8340 tccgtgatcg tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca 8400 aagtcactgg gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg 8460 ttctcttcag ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg 8520 caaacgaagc tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca 8580 tctgctttgg tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag 8640 aaatgggagt tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt 8700 tgggatcaag tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg 8760 gaagtgacac gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg 8820 atcatgtcat acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg 8880 agacctgcct tggactcttc cctggctact ctctctgggc catagatgac gcacagctct 8940 tcaaaggtgt cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg 9000 ctccgagtgg gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg 9060 ttccagccta cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa 9120 ccatgcagcc tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca 9180 agactctctt tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga 9240 gccagcttgt tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg 9300 caactgacac tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg 9360 tctgggtgag gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc 9420 aacacagacc ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg 9480 gtggacatag ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca 9540 ttgatgttca agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa 9600 tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga 9660 tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg 9720 tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta 9780 ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa 9840 gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc 9900 tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg 9960 cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc 10020 ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta 10080 attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc 10140 acttactgat gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctagacc 10260 taggctcagc aactcatcca gtcaagttcg agtcagtgag ttgggcggcc gcagcttata 10320 gtaaagattt ttttgttaat aaaatgaaag ttaatattta tggatttatt tgaacatatt 10380 tacaagtttt tgttaataaa tttatttaca gaagtgttaa atttgaaaat gtgttaaatg 10440 agagttaata tttatggata taatattact ttatttaaaa ataaaatgaa tttatattaa 10500 aatatgattt ggtaaaagat attataaaga ggaacttgga tgagaaagac gacgatgggc 10560 aaaagttgca tggcacaccg cttttgttca gtaacatctg cactgtggcg cctttttcac 10620 actcaccatg catgctgcca cgtcagcttt cacactatga caccactggc catacacgtt 10680 gccacctcag ctttctcctc ttctcactat gacacgactg gccatgcatg catgcatgct 10740 gccacctcag ctcccgcctc ttcacccgtg tctttctctc atgtcgcagt gctgcgtgcc 10800 aacctcttct ctctataaat agagagaaga gaatgatggt taaactcaaa ccaaaacaag 10860 aaaacataca caaatagcaa aacggatcca caaccaaaaa tggctgcaag gaacgtgagt 10920 gcggctcatg agatgcatga cgagaagagg atagctgttg tgggcatggc tgttcagtat 10980 gctggatgca aaacgaaaga cgagttctgg gaagttctca tgaatggaaa agttgagagc 11040 aaagtgatca gcgacaaacg tcttggttca aattaccgtg ctgaacacta caaagctgaa 11100 aggagcaagt atgctgacac cttctgcaat gagacatatg gaactcttga tgagaacgaa 11160 atagacaatg agcatgaact ccttttgaac cttgccaaac aagccttggc tgaaacatct 11220 gtcaaagatt caacaagatg tggcatagtt tctggatgcc tcagcttccc gatggacaac 11280 ctccaaggtg aactcttgaa tgtgtaccag aaccatgttg agaagaaact tggtgccaga 11340 gttttcaaag atgcctctca ctggtcagaa agagaacagt ccaacaaacc agaggctggt 11400 gacagaagaa tcttcatgga ccctgcttcc tttgttgctg aggaactcaa tcttggcgct 11460 ctccactact ctgttgatgc tgcctgtgcc actgcgctct atgtgctcag actggcgcaa 11520 gatcacttgg tctctggtgc agctgatgtg atgctttgtg gtgcaacatg cctgccggag 11580 ccgtttttca tactctctgg gttctcaacc ttccaagcca tgcctgttgg aacgggtcag 11640 aatgtgtcca tgccgctgca caaagattct caaggattga cccctggtga aggaggttcc 11700 atcatggtcc tcaaacgttt ggatgacgca atcagagatg gagatcacat ctatggaaca 11760 cttttgggag ccaatgtcag caacagtggc actggtctgc cacttaaacc ccttttgccg 11820 tcagagaaaa agtgcctcat ggacacctac acacgtatca atgtgcatcc ccacaagatt 11880 cagtatgttg aatgccatgc aactgggact ccacaaggtg atcgtgtgga aattgatgct 11940 gtgaaggctt gctttgaagg caaagtccca cgttttggaa ccacaaaagg gaactttgga 12000 cacacattgg tggcagctgg gtttgctggc atgtgcaaag tgcttctctc catgaagcac 12060 gggatcattc cacccactcc gggaatagat gacgaaacga agatggaccc tctggttgtc 12120 tctggtgagg ccattccctg gccagaaacg aatggagaac cgaagcgtgc tggtctctca 12180 gcgtttggat ttggtgggac caatgctcat gctgtctttg aggaacatga cccaagcaat 12240 gctgcatgca ctggccatga ctccatttct gctctctctg cacgttgtgg tggtgagtca 12300 aacatgagaa tagcaatcac tgggatggat gccacttttg gtgctttgaa aggattggat 12360 gcttttgaaa gggccatcta cactggtgct catggtgcta taccactccc agaaaagaga 12420 tggagattcc tggggaaaga caaggacttc cttgatcttt gtggagtcaa agcaacaccg 12480 catggctgtt acattgaaga tgttgaagtt gatttccaga ggctgaggac tccaatgact 12540 cctgaagata tgctcttgcc tcaacagctt ctggctgtca ccacaattga tagggccata 12600 ttggactctg gcatgaagaa aggtggaaat gtggctgtct ttgtggggtt ggggaccgat 12660 cttgaactct accgtcaccg tgcaagggtt gctctcaagg agagagtgag gcctgaagcc 12720 agcaagaaac tcaatgacat gatgcagtac atcaatgatt gtgggacaag cacttcttac 12780 accagctaca taggcaactt ggttgcaaca agggtcagtt cacaatgggg cttcacggga 12840 ccttccttca caatcacaga aggcaacaat tctgtgtaca gatgtgctga acttggcaaa 12900 tacctcttgg aaacgggaga ggttgatggg gttgtggttg cgggtgttga tctctgtggg 12960 agtgctgaga acctttacgt caagtcaagg cgtttcaagg tgtctacatc tgacaccccg 13020 agagccagct ttgatgcagc tgctgatggt tactttgttg gagagggctg tggtgcattt 13080 gtgctcaagc gtgagacaag ctgcaccaag gatgacagaa tctatgcttg catggatgcc 13140 atagttcctg gaaatgttcc atctgcatgt ttgagagagg cattggatca agccagagtc 13200 aagcctggag acatcgagat gttggagctt tctgctgatt cagcgaggca tttgaaggac 13260 ccgtctgttc tcccaaagga actcactgct gaggaagaga ttggcggact tcagacgatt 13320 cttcgtgacg atgacaagct cccaaggaat gttgcaactg gaagtgtcaa ggccactgtt 13380 ggtgacactg gttatgcttc tggtgctgcc agtttgatta aggctgcgct ttgcatctac 13440 aaccgttacc tgcccagcaa tggagatgac tgggatgaac ctgcacctga agcaccctgg 13500 gacagcacac tctttgcgtg ccagacctca agagcttggt tgaagaaccc tggtgagaga 13560 cgttatgcgg ctgtttccgg agtcagtgaa accagaagtt gctacagtgt gctgctttct 13620 gaagcagagg gacactacga gagagagaac agaatcagtt tggatgagga agcacccaag 13680 ctcattgtgt tgagagcaga ctcccatgaa gagattcttg gaagattgga caagataagg 13740 gaaaggttct tgcagcccac gggtgctgca cccagagagt ctgaattgaa ggcgcaagcc 13800 cgtaggatct ttcttgaact ccttggtgag acccttgccc aagatgcagc ttcatctgga 13860 agtcagaagc cccttgctct cagcttggtc tccactccat ccaagctcca gagagaggtg 13920 gaacttgcgg caaaaggcat tccgagatgt ctcaagatga gacgtgattg gagttcccct 13980 gctggaagca gatatgcgcc tgagcctttg gccagtgaca gagttgcctt catgtatgga 14040 gaaggaagga gcccttacta tggcatcaca caagacattc ataggatttg gcctgaactt 14100 catgaggtca tcaatgagaa aacaaaccgt ctctgggctg aaggtgacag atgggtcatg 14160 ccaagggcca gcttcaagag tgagcttgag tcacaacagc aagagtttga cagaaacatg 14220 attgaaatgt tccgtcttgg aatcctcacc tcaattgcct tcacaaacct ggcgagagat 14280 gttctcaaca tcacgcccaa agcagccttt ggactcagtt tgggagagat ttccatgatc 14340 tttgctttct ccaagaaaaa cggtttgata agcgatcagt tgacaaagga tttgagagag 14400 tcagatgtct ggaacaaagc tctggctgtt gagttcaatg cgctcagaga agcctggggc 14460 attccacagt ctgtcccaaa agatgagttc tggcaaggtt acatagtgag gggaacgaaa 14520 caagacatag aagcagccat agcaccagac tcaaagtatg tgaggttgac catcatcaat 14580 gatgccaaca ctgctctcat cagtggaaag cctgatgcat gcaaagctgc gattgctcgt 14640 cttggtggca acatccctgc gcttccagtc actcaaggca tgtgtgggca ttgcccagag 14700 gtgggacctt acaccaagga catcgcaaag atccacgcca accttgagtt ccctgtggtt 14760 gatggtcttg atctctggac taccataaac cagaagaggc ttgtgccaag agccacgggt 14820 gccaaagatg aatgggctcc ttcatccttt ggggagtatg ctggacagct ctatgagaaa 14880 caagccaact tccctcagat agttgaaacc atctacaagc agaactatga tgtgtttgtt 14940 gaagttgggc caaacaatca cagaagcaca gcagtgagaa ccacgcttgg accacagaga 15000 aaccaccttg ctggtgccat agacaagcag aacgaggatg cttggacgac aatagtcaag 15060 ctcgtggctt cattgaaggc tcaccttgtt cctggagtga ccatcagtcc gctctaccac 15120 tccaagctcg ttgcagaggc tgaggcttgt tatgctgcgc tctgtaaagg tgagaaaccc 15180 aagaaaaaca agtttgtgag aaagattcag ttgaatggca gattcaacag caaggcagac 15240 cccatctcct cagcagacct tgcatccttc ccacctgcgg accctgcgat tgaagctgcc 15300 atctcttcaa ggatcatgaa gcctgtggct ccgaaattct atgcgcgtct caacattgat 15360 gagcaagatg agaccagaga tccgatcttg aacaaggaca atgcgccttc cagctccagc 15420 tcttccagca gttcctcttc cagcagttca tctccgtctc ctgctccttc agctccagtt 15480 cagaagaaag ctgccccagc tgcggagaca aaagctgttg ccagtgctga tgcacttcgt 15540 agtgccctgc ttgatcttga ctccatgctt gcgctcagct ctgcaagtgc ttctggcaac 15600 cttgttgaga ctgcgccatc tgatgccagt gtcattgtcc ctccgtgcaa cattgctgat 15660 cttggcagca gagctttcat gaaaacttat ggtgtttctg cgcctctgta caccggtgca 15720 atggccaagg gaattgcctc tgcggacttg gtgatcgcag ctggaaggca aggaatcctt 15780 gcgtcatttg gtgctggagg ccttccaatg caagttgtgc gtgagtccat tgaaaagatt 15840 caagctgccc tgcccaatgg accatatgct gtcaacctca tccattctcc ttttgacagc 15900 aaccttgaga agggaaatgt tgatctcttc ttggaaaagg gtgtcacttt tgttgaggct 15960 tcagccttca tgacgttgac accgcaagtt gtcagataca gagcggctgg gttgacgagg 16020 aatgcagatg ggtcagtcaa cataaggaac agaatcattg ggaaagtctc cagaactgag 16080 ctggctgaaa tgttcatgcg tcctgcacct gagcatcttt tgcagaagct cattgcttct 16140 ggggagatca atcaagaaca agctgagttg gccagacgtg ttcctgtggc tgatgacatt 16200 gcggttgaag ctgattctgg tggacacact gacaaccgtc caatccatgt cattctgcct 16260 ctgattatca acctgaggga taggcttcac agagagtgtg gatacccagc aaacctgagg 16320 gttcgtgttg gtgctggggg tggcattggg tgtccccaag ctgcgctggc aactttcaac 16380 atgggtgcct ctttcattgt cactggaaca gtgaaccaag ttgccaaaca atctgggacg 16440 tgtgacaatg tgaggaaaca gcttgcgaaa gcaacttact cagatgtttg catggcaccg 16500 gcagctgaca tgtttgagga aggtgtgaaa cttcaagttt tgaagaaagg aacaatgttc 16560 ccttcaagag ccaacaaact ctatgagttg ttctgcaagt atgattcttt tgagtccatg 16620 cctccagcag agcttgcgag ggttgagaaa agaatcttca gtcgtgcatt ggaagaggtc 16680 tgggatgaga ccaagaactt ctacatcaac cgtctccaca acccagagaa gatacagagg 16740 gctgagaggg accccaaact gaagatgtca ctgtgtttca gatggtatct gtccctggct 16800 tcaaggtggg ccaacactgg agcttctgac agagtcatgg actaccaagt ttggtgtggt 16860 cctgccattg gttcattcaa tgatttcatc aaaggaactt accttgatcc ggcagtggca 16920 aatgagtacc cgtgtgttgt gcagatcaac aaacagattc ttagaggagc gtgcttcttg 16980 aggcgtcttg aaatactgag aaatgcaagg ctttcagatg gtgcagctgc acttgtggcg 17040 agcattgatg acacatatgt ccctgctgag aagctgtgag tagttagctt aatcacttag 17100 gtcacccagt ccaagcccgg cctagcacga tgatcatgag tgttgctatt gatgtatgtt 17160 aatactatac acagtcatcg tgtgtgtttt ataaaactgt aagcttagga tgtttgaggc 17220 taatgtaatt agcactacac cgtaataaat gagaggttta ctttatgttt taataatgtt 17280 cttacccttg ctgctctcta aactgtaatg gcgtacggtt agctaaacta aagtaaaaat 17340 cgattcgctt gagtaaaatt agtgaccggg aaagatcatc gactactact gctcagagat 17400 gtaatcggta attaagactt caagctcaat aattaattag gagtaaatct ggcttcgaat 17460 ttttaaattg tcaacttcca aagaaggatt ggcaagactc aatctcaatt gcccaacaaa 17520 catatggctc tttacaaatt tggaatctct ttttgatggc aagaacgtcg taattcctct 17580 gtcgatctca tgtaattacc aaactcatct acttattact ttctttatat gcacttactg 17640 atgcggccgc ttaattaatc tgacctgacc actagacctg agtgagtaca gatgtgctac 17700 tagtgctagc ctcgaggtcg accaactttt ctatacaaag ttgtctagac ctaggactag 17760 gcactgagtg actcactgac ctgactgagt gactggcggc cgcagcttat agtaaagatt 17820 tttttgttaa taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt 17880 ttgttaataa atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat 17940 atttatggat ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt 18000 tggtaaaaga tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc 18060 atggcacacc gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat 18120 gcatgctgcc acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca 18180 gctttctcct cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca 18240 gctcccgcct cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc 18300 tctctataaa tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac 18360 acaaatagca aaacggatcc acaaccaaaa atggcgcttc gtgtcaagac caacaagaag 18420 ccatgttggg agatgaccaa ggaggaactg acctctggca aaactgaggt gttcaactat 18480 gaggaactcc tggagtttgc agagggtgac atagctaagg tttttggtcc ggagtttgct 18540 gtcatagaca agtaccccag aagggtgagg ctgcctgctc gtgaatacct gcttgtgaca 18600 agggtcacac tcatggatgc cgaggtcaac aactacagag ttggtgccag aatggtcact 18660 gagtatgatc ttccagtgaa tggcgaattg tctgagggag gtgattgtcc ctgggcagtt 18720 ctggttgaga gtggacagtg cgatttgatg ctgatatcat acatgggaat tgatttccag 18780 aaccaaggtg acagagttta ccgtctgctc aacaccacgt tgacattcta tggggttgcc 18840 catgagggag agacccttga gtatgacatc agagtgaccg gttttgccaa acgtttggat 18900 ggtggcatct ccatgttctt ctttgagtat gactgctatg tcaatgggag gcttctcatt 18960 gagatgagag atggctgtgc tggcttcttc acaaatgagg agcttgatgc gggaaagggt 19020 gtggtgttca caagagggga tttggctgcc agagctaaga ttccaaagca agatgtttct 19080 ccctatgctg tcgctccctg ccttcacaaa acaaaactca acgaaaagga gatgcagacc 19140 ttggttgaca aagattgggc ttccgttttt ggctcaaaga atggcatgcc ggagatcaac 19200 tacaaactct gcgcacgtaa gatgctcatg attgatagag tcaccagcat tgaccataag 19260 ggaggtgtgt acggtttggg tcagttggtt ggtgagaaaa tcttggagag ggaccattgg 19320 tacttcccat gccattttgt taaggatcaa gtcatggctg gatctcttgt cagcgacggt 19380 tgttcacaaa tgctcaagat gtacatgatc tggctcggtt tgcaccttac caccggacca 19440 tttgacttca gacccgtcaa cggacacccg aacaaagtta ggtgtagggg tcagatatct 19500 cctcataagg gaaagttggt ctacgtcatg gagatcaaag agatgggatt cgacgaggat 19560 aacgatccgt acgccattgc tgacgtcaac atcattgacg ttgatttcga aaagggtcaa 19620 gatttcagcc ttgatagaat cagcgactac ggaaaggggg atcttaacaa aaagatcgtc 19680 gtggacttca agggcattgc tctcaagatg cagaagagat ccacgaacaa gaacccctcc 19740 aaagttcagc ccgtttttgc taacggtgca gccactgtgg gaccggaagc gtccaaggcc 19800 agctctggtg ccagtgctag tgcttctgct gcccctgcga agcctgcttt ctcagcagat 19860 gttcttgcgc caaagccagt tgcacttcct gaacatatcc tcaagggtga cgctctcgct 19920 ccgaaagaaa tgtcctggca tcccatgtca aaacttcctg gcaacccgac gccgttcttc 19980 tcccctagtt cttaccctcc gagggcaatt tgcttcatcc ctttccctgg caacccactt 20040 gacaacaatt gcaaagctgg agaaatgcct ctgaactggt acaacatgtc agagttcatg 20100 tgtggaaaag ttagcaactg cttgggacca gagttcgcaa gatttgataa gtcaaatacg 20160 agcagaagcc ctgcttttga tttggctctt gtgactagag ttgttgaagt cacaaacatg 20220 gaacatggca agttcctgaa tgttgattgc aatcccagca aaggcacaat ggtgggggag 20280 tttgactgtc ctcaagatgc gtggttcttt gatggttctt gcaatgatgg gcacatgcct 20340 tattccatca tcatggaaat cggacttcag acatccggtg ttctcaccag tgtgttgaaa 20400 gcaccactga cgatggacaa agatgacatt ctctttagga accttgatgc aagtgctgaa 20460 atggtgaggc cagatgtgga tgtgaggggg aaaaccataa ggaatgtgac caagtgcact 20520 ggatatgcaa tgttgggaaa gatggggatt cacagattca cgtttgagtt gtctgttgat 20580 ggcgtggttt tctacaaagg aagcacttcc tttggatggt tcactccaga ggtgtttgct 20640 cagcaagctg gacttgacaa tgggaaaaag acggagcctt ggtgcaaaac taacaacacg 20700 agtgtgagaa gagttgaaat cgcatcagcc aaaggcaaag aacagctgac tgagaaactt 20760 ccagatgcaa ctaatgctca agttctcaga aggtcagaac agtgtgagta tttggattac 20820 ctcaacattg ctcctgattc tgggctgcat gggaaaggat atgcgcatgg acacaaagat 20880 gttaacccac aagactggtt cttctcttgt cacttttggt ttgatcctgt gatgcctgga 20940 tctttgggaa ttgagtcaat gttccagctc attgaggcat ttgcggtgga ccagaacata 21000 ccgggagaat acaatgtgag caatccgacc tttgcccatg cccctggaaa aacggcgtgg 21060 aagtaccgtg gtcaattgac accaaagaac cgtgcgatgg actgtgaggt gcacatagtt 21120 tcaatcacag cctctcctga aaatggaggc tacgttgaca tagtggcaga tggagcactt 21180 tgggttgatg gtctccgtgt gtatgaagcc aaagaactca gagttcgtgt tgtcagtgca 21240 aaacctcaag ccattccgga tgttcaacaa cagccaccca gcgcaaaagc ggaccctggg 21300 aaaactggag ttgcactcag tccgactcag ttgagagatg tcctgcttga agtggacaat 21360 ccattgtatc ttggtgttga aaactccaat ttggtgcagt ttgaatccaa acctgcaact 21420 tcttcaagga ttgtcagtat caaaccatgc tccataagtg atcttggtga caagtccttc 21480 atggaaacgt acaatgtgtc agcacctctg tacactggag caatggccaa gggaattgcg 21540 tctgcggacc ttgtcattgc tgctgggaag aggaagatat tgggttcttt tggagcgggt 21600 gggcttccaa tgcaccatgt gagggcagct ttggagaaga tacaagcggc tctgcctcaa 21660 ggaccatatg ctgtgaacct cattcattca ccttttgaca gcaacttgga aaaggggaat 21720 gttgatctct tcctcgaaaa gggcgttact gtggtggagg cttctgcatt catgaccctc 21780 actccccaag ttgttcgtta ccgtgcagcc ggactctcaa ggaacgctga tgggagtgtc 21840 aacatcagaa accgtatcat tggaaaagtg tccagaacag aactcgccga aatgttcatc 21900 agaccggctc cggaacacct ccttgagaaa ctcatagctt ctggtgagat aacacaagaa 21960 caagccgaac ttgcccgtcg tgttcctgtc gcagacgaca tcgctgttga agctgattct 22020 ggtggtcaca ctgacaaccg tcccatccac gtcatcttgc ctttgatcat caaccttagg 22080 aacagactgc acagagagtg tggttatcct gcgcacctta gagtcagagt tggagctgga 22140 ggcggtgttg gctgcccaca agctgctgcg gctgccctca caatgggagc tgccttcata 22200 gtgactggga ctgtcaatca agttgctaaa cagtccggga catgtgacaa cgtgagaaaa 22260 caactcagtc aagccaccta cagtgacatt tgcatggctc cagcagctga tatgtttgag 22320 gaaggtgtga aactccaagt tctcaagaag ggaacaatgt tcccatcaag agctaacaaa 22380 ttgtacgaac tcttttgcaa gtacgactct ttcgattcaa tgcctcctgc tgaacttgaa 22440 aggatcgaga agaggatctt caagagggca cttcaagagg tctgggaaga gaccaaggac 22500 ttttacatca atggtttgaa gaatccagag aagatacaga gagcggaaca tgaccccaaa 22560 ctcaagatgt ctttgtgttt cagatggtat cttggtcttg catcacgttg ggccaacatg 22620 ggtgctcctg atcgtgtcat ggactatcaa gtctggtgtg gtccagccat tggagccttc 22680 aatgacttca tcaaaggaac atatcttgat cctgctgtct ctaatgagta cccatgtgtt 22740 gtccagatca accttcagat tttgcgtggt gcatgttacc tgaggcgtct taatgctctg 22800 agaaatgatc cgaggattga tcttgagact gaagatgctg cctttgtcta tgagccaacg 22860 aatgcgcttt gagtagttag cttaatcact taggtcaccc agtccaagcc cggcctagca 22920 cgatgatcat gagtgttgct attgatgtat gttaatacta tacacagtca tcgtgtgtgt 22980 tttataaaac tgtaagctta ggatgtttga ggctaatgta attagcacta caccgtaata 23040 aatgagaggt ttactttatg ttttaataat gttcttaccc ttgctgctct ctaaactgta 23100 atggcgtacg gttagctaaa ctaaagtaaa aatcgattcg cttgagtaaa attagtgacc 23160 gggaaagatc atcgactact actgctcaga gatgtaatcg gtaattaaga cttcaagctc 23220 aataattaat taggagtaaa tctggcttcg aatttttaaa ttgtcaactt ccaaagaagg 23280 attggcaaga ctcaatctca attgcccaac aaacatatgg ctctttacaa atttggaatc 23340 tctttttgat ggcaagaacg tcgtaattcc tctgtcgatc tcatgtaatt accaaactca 23400 tctacttatt actttcttta tatgcactta ctgatgcggc cgcttaatta atcacgagtg 23460 gatgactgtc acgactgact catgactgac tactagtgct agcctcgagg tcgacacaac 23520 tttgtataat aaagttgtct agacctaggc gagtcatgag tcgtgacatg acgtcatgct 23580 catcagactg cggccgcagc ttatagtaaa gatttttttg ttaataaaat gaaagttaat 23640 atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat ttacagaagt 23700 gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata ttactttatt 23760 taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat aaagaggaac 23820 ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt gttcagtaac 23880 atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca gctttcacac 23940 tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc actatgacac 24000 gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac ccgtgtcttt 24060 ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga gaagagaatg 24120 atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg atccacaacc 24180 aacctaccat ggctcccact cccgacgcca ccgcgcctct gaacaagccg agcgactatg 24240 ccgtctacca cgaggaagac ggccccttct ggaccgccga ttccagcggc gtctcgcgcg 24300 tgaactttag cgagaccggc gtgggatccg agggcgtcat ccctgcgctc acgctcatcg 24360 acgtcttcga gagggccgtc aagcgcggcg gaaacaggat cgccttccgc acggaaaaca 24420 tgcccacgct ccgccgcggc gaagaggccc cggacgcgct gccgctcaag gactggaagt 24480 cctggtcctg gaaacagtac aaggccgacg tccaccgcat cgccagggct ctcatggacc 24540 tcggcgttga gcagcatgac gccgtctcca tttttggctt taactcgccc gaatggtttc 24600 tcagtgccgt cggcgccgtg cacgcaggtg ccaagattgc cggcatttac ccctcagaca 24660 cgcccgccca ggtccagtac aaggccttcc acagtgacac cgctgttgcc gttgtcgaaa 24720 acgagcagtg cttcaagaag ttcgccgagg tcgtcgagga ccttccttac ctcaaggcca 24780 ttgtttgctg ggactatgaa gccacagaca tcacgcgcga ggacggctcc gtcgtcgagg 24840 tcctcacctt tgccgagttc ctcaagcgcg gcgacaccgt cgaggcggcc gcccttgacg 24900 agcgcatctc caagatcgag cccaccatgt gcgctgccct tatttacacc agcggtacta 24960 ccggccgccc caaggccgtt atgatttcgc acgacaacct tgttttcgag gccagcgccg 25020 tcgtccccaa cctcggagga gcctgtacga ccactgctga ggagcgcatt ctctcgtacc 25080 tgcctctctc gcacgtcgct ggtatgatgg ttgatattat tgcccccatc attgccaccg 25140 ccttccacaa gggccgcatc tgcgtctgct ttgctcgccc gtacgatttg cgcaccggca 25200 cgctgggcca gcgcctcaac gccgtggagc ccaccatctt ccttggcgtg ccccgtgtgt 25260 gggaaaagat tcaggaaaag ctcatggccg tcggtgccaa aaccaccggc ctcaagaaga 25320 agctctctac ggccgccaag aagcgtggtc ttgaattcca ggaggagcag caaatcggcc 25380 gctccggtgc caaccctggc tttggccccc ttggcatcta caagaagctc ctcggcctca 25440 tcaagggcaa gctgggcctc accaagtgca agtttgcctt tgctggtgcc gcgcccatga 25500 cccgtgagac ccttcagtac tttggcgcgc tgaacatcaa cattaacgag gtctacggca 25560 tgtccgagtg ctccggtgcc gccacctggt ccacggacaa ggcccacgag tggggcactg 25620 ttggctacga gatgcccagt tgcgaggtcc gcgtcttcaa gattgccgag gacggtacca 25680 agaccgagtg cccgcgcgcc gccgacatta tgcatgctac cgaggaggag cagggcgaag 25740 tttgcttccg cggccgtaac atcatgatgg gctaccttgc caaccccaag cttggcgacg 25800 accacgttgc cgagatcgag gagaagaacg ctgccgctat cgactccgag ggctggctcc 25860 acagtggtga taagggcgcc atttctaccc gcggcatgct caagatcacg ggccgctaca 25920 aggagctcat catcggcgcc ggtggcgaga acgtggcgcc cgtccctatt gaggacgcca 25980 tcaaggcgcg catgcctttt gtttccaacg ccatgatggt cggagataag cgcaagttca 26040 tggctgtcct ccttaccctc aagacggttg gcgccacggg cgagcttccc ggtacgaaca 26100 agctcatggg cgctgccgcc gactatggtg agaccatcga ggacgcctgc gacaacgagg 26160 cgctcattga ggagatcacg cagcagctca aggagatcgg tgatgatggc gatgtcacgc 26220 cctcgaacgc ggctcgcatc caaaagttca ccattctccc gctcgacttt tccgtctcca 26280 cggacgagct cacggccacg ctcaagctca agcgctccgt ggtcgcagac aagtacgaag 26340 acatcatcga ggccttttac gagtccaaga gcgtttttgt gccgtactcg accgttggcg 26400 cctacgccac gggcggcccg gtcgacgact ccgttgtcga tggctccttc aagggcgact 26460 ttagcatgat tggcgacgat gatccggatc ttcaaaacgt cgatgtcctc gaggcgattg 26520 acgaggacaa ttaaatttcc agtccaagcc cggcctagca cgatgatcat gagtgttgct 26580 attgatgtat gttaatacta tacacagtca tcgtgtgtgt tttataaaac tgtaagctta 26640 ggatgtttga ggctaatgta attagcacta caccgtaata aatgagaggt ttactttatg 26700 ttttaataat gttcttaccc ttgctgctct ctaaactgta atggcgtacg gttagctaaa 26760 ctaaagtaaa aatcgattcg cttgagtaaa attagtgacc gggaaagatc atcgactact 26820 actgctcaga gatgtaatcg gtaattaaga cttcaagctc aataattaat taggagtaaa 26880 tctggcttcg aatttttaaa ttgtcaactt ccaaagaagg attggcaaga ctcaatctca 26940 attgcccaac aaacatatgg ctctttacaa atttggaatc tctttttgat ggcaagaacg 27000 tcgtaattcc tctgtcgatc tcatgtaatt accaaactca tctacttatt actttcttta 27060 tatgcactta ctgatgcggc cgcttaatta atctgatcat ggatgctacg tcacggcagt 27120 acaggactat ccctaggagc tgtgctgggt aactcacatg agtcactggt cacatcagcg 27180 gccgcagctt atagtaaaga tttttttgtt aataaaatga aagttaatat ttatggattt 27240 atttgaacat atttacaagt ttttgttaat aaatttattt acagaagtgt taaatttgaa 27300 aatgtgttaa atgagagtta atatttatgg atataatatt actttattta aaaataaaat 27360 gaatttatat taaaatatga tttggtaaaa gatattataa agaggaactt ggatgagaaa 27420 gacgacgatg ggcaaaagtt gcatggcaca ccgcttttgt tcagtaacat ctgcactgtg 27480 gcgccttttt cacactcacc atgcatgctg ccacgtcagc tttcacacta tgacaccact 27540 ggccatacac gttgccacct cagctttctc ctcttctcac tatgacacga ctggccatgc 27600 atgcatgcat gctgccacct cagctcccgc ctcttcaccc gtgtctttct ctcatgtcgc 27660 agtgctgcgt gccaacctct tctctctata aatagagaga agagaatgat ggttaaactc 27720 aaaccaaaac aagaaaacat acacaaatag caaaacggat ccacaaccaa aaatgcttca 27780 gcacacttgg cttccgaagc ctcccaatct gaccctcttg tcagatgagg ttcatctctg 27840 gaggattcct cttgaccagc ctgagtcaca acttcaagac cttgctgcca ccctgagcag 27900 tgatgaattg gcgagggcaa acagattcta ctttccagaa cacagaaggc gtttcactgc 27960 tgggagaggc atcctcagat ccatcttggg tggatacttg ggagtggaac cgggtcaagt 28020 caagtttgat tatgagtccc gtgggaaacc gatccttggt gacagatttg ctgagagtgg 28080 actcctgttc aacttgtctc acagccagaa ccttgccttg tgtgctgtca actacacgcg 28140 tcaaataggc attgatcttg aatatctgcg tccaacatct gacttggagt ctcttgcaaa 28200 gaggttcttt ctcccaagag aatatgaact cttgaggtca ctccctgatg agcagaaaca 28260 gaagattttc tttcgttact ggacttgcaa agaggcttat ctcaaagcaa cgggagatgg 28320 aatagccaaa cttgaagaga tcgagatagc actcacccca acagaacctg ccaagctcca 28380 aacagctcct gcgtggtctc tgttggagct tgtgccagat gacaattgtg ttgcagctgt 28440 ggctgttgcg ggttttggtt ggcagcccaa gttctggcat tactgagtag ttagcttaat 28500 cacttaggtc acccagtcca agcccggcct agcacgatga tcatgagtgt tgctattgat 28560 gtatgttaat actatacaca gtcatcgtgt gtgttttata aaactgtaag cttaggatgt 28620 ttgaggctaa tgtaattagc actacaccgt aataaatgag aggtttactt tatgttttaa 28680 taatgttctt acccttgctg ctctctaaac tgtaatggcg tacggttagc taaactaaag 28740 taaaaatcga ttcgcttgag taaaattagt gaccgggaaa gatcatcgac tactactgct 28800 cagagatgta atcggtaatt aagacttcaa gctcaataat taattaggag taaatctggc 28860 ttcgaatttt taaattgtca acttccaaag aaggattggc aagactcaat ctcaattgcc 28920 caacaaacat atggctcttt acaaatttgg aatctctttt tgatggcaag aacgtcgtaa 28980 ttcctctgtc gatctcatgt aattaccaaa ctcatctact tattactttc tttatatgca 29040 cttactgatg cggccgctta attaactact gtcactgagg ccgtagacga gtacggactg 29100 atctaactag tgctagcctc gaggtcgaca cccagctttc ttgtacaaag tggttgcggc 29160 cgcttaatta aatttaaatt caattaatgc aatcttgatt ttcaacaacg aaggtaatgg 29220 cgtaaaagaa aaaatgtatg ttattgtatt gatctttcat gatgttgaag cgtgccataa 29280 tatgatgatg tataattaaa atattaactg tcgcatttta ttgaaatggc actgttattt 29340 caaccatatc tttgattctg ttacatgaca cgactgcaag aagtaaataa tagacgccgt 29400 tgttaaagaa ttgctatcat atgtgcctaa ctagagggaa tttgagcgtc agacctaatc 29460 aaatattaca aaatatctca ctctgtcgcc agcaatggtg taatcagcgc agacaaatgg 29520 cgtaaagatc gcggaaaaac ctccccgagt ggcatgatag ctgcctctgt attgctgatt 29580 tagtcagcct tatttgactt aagggtgccc tcgttagtga caaattgctt tcaaggagac 29640 agccatgccc cacactttgt tgaaaaacaa attgcctttg gggagacggt aaagccagtt 29700 gctcttcaat aaggaatgtc gaggaggcaa tgtaaccgcc tctggtagta cacttctcta 29760 atccaaaaat caatttgtat tcaagatacc gcaaaaaact tatggtttaa accctgcagg 29820 actagtccag aaggtaatta tccaagatgt agcatcaaga atccaatgtt tacgggaaaa 29880 actatggaag tattatgtaa gctcagcaag aagcagatca atatgcggca catatgcaac 29940 ctatgttcaa aaatgaagaa tgtacagata caagatccta tactgccaga atacgaagaa 30000 gaatacgtag aaattgaaaa agaagaacca ggcgaagaaa agaatcttga agacgtaagc 30060 actgacgaca acaatgaaaa gaagaagata aggtcggtga ttgtgaaaga gacatagagg 30120 acacatgtaa ggtggaaaat gtaagggcgg aaagtaacct tatcacaaag gaatcttatc 30180 ccccactact tatcctttta tatttttccg tgtcattttt gcccttgagt tttcctatat 30240 aaggaaccaa gttcggcatt tgtgaaaaca agaaaaaatt tggtgtaagc tattttcttt 30300 gaagtactga ggatacaact tcagagaaat ttgtaagttt gtaggtacca gatctggatc 30360 ccaaaccatg tctccggaga ggagaccagt tgagattagg ccagctacag cagctgatat 30420 ggccgcggtt tgtgatatcg ttaaccatta cattgagacg tctacagtga actttaggac 30480 agagccacaa acaccacaag agtggattga tgatctagag aggttgcaag atagataccc 30540 ttggttggtt gctgaggttg agggtgttgt ggctggtatt gcttacgctg ggccctggaa 30600 ggctaggaac gcttacgatt ggacagttga gagtactgtt tacgtgtcac ataggcatca 30660 aaggttgggc ctaggatcta cattgtacac acatttgctt aagtctatgg aggcgcaagg 30720 ttttaagtct gtggttgctg ttataggcct tccaaacgat ccatctgtta ggttgcatga 30780 ggctttggga tacacagccc ggggtacatt gcgcgcagct ggatacaagc atggtggatg 30840 gcatgatgtt ggtttttggc aaagggattt tgagttgcca gctcctccaa ggccagttag 30900 gccagttacc caaatctgag tagttagctt aatcacctag agctcgatcg gcggcaatag 30960 cttcttagcg ccatcccggg ttgatcctat ctgtgttgaa atagttgcgg tgggcaaggc 31020 tctctttcag aaagacaggc ggccaaagga acccaaggtg aggtgggcta tggctctcag 31080 ttccttgtgg aagcgcttgg tctaaggtgc agaggtgtta gcgggatgaa gcaaaagtgt 31140 ccgattgtaa caagatatgt tgatcctacg taaggatatt aaagtatgta ttcatcacta 31200 atataatcag tgtattccaa tatgtactac gatttccaat gtctttattg tcgccgtatg 31260 taatcggcgt cacaaaataa tccccggtga ctttctttta atccaggatg aaataatatg 31320 ttattataat ttttgcgatt tggtccgtta taggaattga agtgtgcttg aggtcggtcg 31380 ccaccactcc catttcataa ttttacatgt atttgaaaaa taaaaattta tggtattcaa 31440 tttaaacacg tatacttgta aagaatgata tcttgaaaga aatatagttt aaatatttat 31500 tgataaaata acaagtcagg tattatagtc caagcaaaaa cataaattta ttgatgcaag 31560 tttaaattca gaaatatttc aataactgat tatatcagct ggtacattgc cgtagatgaa 31620 agactgagtg cgatattatg gtgtaataca taggaattcg tttaaacgat ctgcgtctaa 31680 ttttcggtcc aacttgcaca ggaaagacgt cgaccgcggt agctcttgcc cagcagactg 31740 ggcttccagt cctttcgctc gatcgggtcc aatgttgtcc tcagctgtga accggaagcg 31800 gacgaccaac agtggaagaa ctgaaaggaa cgagccgtct ataccttgat gatcggcctc 31860 tggtgaaggg tatcatcgca gccaagcaag ctcatgaaag gctgatgggg gaggtgtata 31920 attatgaggc ccacggcggg cttattcttt agggaggatc tatctcgttg ctcaagtgca 31980 tggcgcaaag cagttattgg agtgcggatt ttcgttggca tattattcgc cacgagttag 32040 cagacgaaga gaccttcatg aacgtggcca aggccagagt taagcagatg ttacgccctg 32100 ctgcaggcct ttctattatc caatagttgg ttgatctttg gaaagagcct cggctgaggc 32160 ccatactgaa agagatcgat ggatatcgat atgccatgtt gtttgctagc cagaaccaga 32220 tcacatccga tatgctattg cagcttgacg cagatatgga ggataagttg attcatggga 32280 tcgctcagga gtagctcatc catgcacgcc gacaagaaca gaaattccgt cgagttaacg 32340 cagccgctta cgacggattc gaaggtcatc cattcggaat gtattagttt gcaccagctc 32400 cgcgtcacac ctgtcttcat ttgaataaga tgttagcaat tgtttttagc tttgtcttgt 32460 tgtggcaggg cggcaagtgc ttcagacatc attctgtttt caaattttat gctggagaac 32520 agcttcttaa ttcctttgga aataatagac tgcgtcttaa aattcagatg tctggatata 32580 gatatgattg taaaataacc tatttaagtg tcatttagaa cataagtttt atgaatgttc 32640 ttccattttc gtcatcgaac gaataagagt aaatacacct tttttaacat tacaaataag 32700 ttcttatacg ttgtttatac accgggaatc atttccatta ttttcgcgca aaagtcacgg 32760 atattcgtga aagcgacata aactgcgaaa tttgcgggga gtgtcttgag tttgcctcga 32820 ggctagcgca tgcacataga cacacacatc atctcattga tgcttggtaa taattgtcat 32880 tagattgttt ttatgcatag atgcactcga aatcagccaa ttttagacaa gtatcaaacg 32940 gatgtgactt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa 33000 tttgatttac aattgaatat atcctgcccc agccagccaa cagctcgatt tacaattgaa 33060 tatatcctgc cggccggccc acgcgtgtcg aggaattctg atctggcccc catttggacg 33120 tgaatgtaga cacgtcgaaa taaagatttc cgaattagaa taatttgttt attgctttcg 33180 cctataaata cgacggatcg taatttgtcg ttttatcaaa atgtactttc attttataat 33240 aacgctgcgg acatctacat ttttgaattg aaaaaaaatt ggtaattact ctttcttttt 33300 ctccatattg accatcatac tcattgctga tccatgtaga tttcccggac atgaagccat 33360 ttacaattga atatatcctg ccgccgctgc cgctttgcac ccggtggagc ttgcatgttg 33420 gtttctacgc agaactgagc cggttaggca gataatttcc attgagaact gagccatgtg 33480 caccttcccc ccaacacggt gagcgacggg gcaacggagt gatccacatg ggacttttaa 33540 acatcatccg tcggatggcg ttgcgagaga agcagtcgat ccgtgagatc agccgacgca 33600 ccgggcaggc gcgcaacacg atcgcaaagt atttgaacgc aggtacaatc gagccgacgt 33660 tcacgcggaa cgaccaagca agcttggctg ccatttttgg ggtgaggccg ttcgcggccg 33720 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa 33780 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa 33840 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa 33900 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc 33960 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct 34020 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt 34080 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct 34140 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg 34200 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt 34260 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt 34320 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg 34380 gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat aaccctgctt cggggtcatt 34440 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg 34500 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta 34560 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc 34620 aacgggaatc ctgctctgcg aggctggccg gctaccgccg gcgtaacaga tgagggcaag 34680 cggatggctg atgaaaccaa gccaaccagg aagggcagcc cacctatcaa ggtgtactgc 34740 cttccagacg aacgaagagc gattgaggaa aaggcggcgg cggccggcat gagcctgtcg 34800 gcctacctgc tggccgtcgg ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac 34860 gtccgcgagc tggcccgcat caatggcgac ctgggccgcc tgggcggcct gctgaaactc 34920 tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg 34980 gcgaagatcg aagagaagca ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg 35040 agggcagagc catgactttt ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa 35100 gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg gagctggtat tcgtgcaggg 35160 caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga ccgacttcat 35220 tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc aggaataagg 35280 gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga atcggacgtt 35340 tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg aggatgccga 35400 aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc 35460 gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc 35520 cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg 35580 tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga agcgaaaaac 35640 cgccggcgag gacctggcaa aacaggtcag cgaggccaag caggccgcgt tgctgaaaca 35700 cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc cgtggccgga 35760 cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa 35820 gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa 35880 gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt ggcagcaggt 35940 gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt tctacgagct 36000 ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg aggaatgcct 36060 gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc acctggaatc 36120 ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca 36180 ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca cgaaattcat 36240 atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact atttcagctc 36300 gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg gatcggattc 36360 cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag 36420 cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac gctagggcct 36480 tgtggggtca gttccggctg ggggttcagc agccagcgct ttactggcat ttcaggaaca 36540 agcgggcact gctcgacgca cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc 36600 tacgaactgc cgataaacag aggattaaaa ttgacaattg tgattaaggc tcagattcga 36660 cggcttggag cggccgacgt gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa 36720 gctccagaga tgttcgggtc cgtttacgag cacgaggaga aaaagcccat ggaggcgttc 36780 gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca tcgacggcga gatcattggg 36840 ctgtcggtct tcaaacagga ggacggcccc aaggacgctc acaaggcgca tctgtccggc 36900 gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg 36960 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg 37020 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg 37080 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc 37140 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct 37200 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct 37260 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc 37320 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga 37380 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga 37440 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt 37500 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc 37560 ccccgagcgc ttagtgggaa tttgtacccc ttatcgaacc gggagcacag gatgacgcct 37620 aacaattcat tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca 37680 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 37740 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 37800 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 37860 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 37920 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 37980 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 38040 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 38100 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 38160 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 38220 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 38280 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 38340 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc 38400 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg 38460 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa 38520 gcgttagaga gctggggaag actatgcgcg atctgttgaa ggtggttcta agcctcgtct 38580 tgcgatggca tttcgatcca ttcccattcc gcgctcaaga tggcttcccc tcggcagttc 38640 atcagggcta aatcaatcta gccgacttgt ccggtgaaat gggctgcact ccaacagaaa 38700 caatcaaaca aacatacaca gcgacttatt cacacgagct ca 38742 <210> 45 <211> 38760 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB101477 <400> 45 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga 900 ggattgcgat cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat 960 gggagaccat aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg 1020 tgactgcgta ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag 1080 gtttcattcc tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg 1140 aagattctga tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg 1200 ctgggattga tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag 1260 gtggacagaa gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag 1320 tcttgagaaa gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag 1380 cgaacttccc agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg 1440 gcagatgcac caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg 1500 caagctcctt gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca 1560 tgatggtcac tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa 1620 agacacctgt gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga 1680 tgctcattgg tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg 1740 atggtgatga gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag 1800 ctggcatcta cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc 1860 gtgcctgcgt tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag 1920 ttggagatag gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag 1980 ggaacacaga gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg 2040 ttgctggtct tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg 2100 gaacgatcaa tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat 2160 ccctctacat caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg 2220 ctggaatatc cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg 2280 aaccagagca cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg 2340 ctgcaactcc tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg 2400 cgatcaagga gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag 2460 aacagttcaa gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca 2520 aagatgcgga ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg 2580 tgaccaaaga agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg 2640 ccaccaatgg tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt 2700 tcagtgaagt tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg 2760 cacagtccaa ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc 2820 caaggaaacc gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat 2880 acagccagcc gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg 2940 gattcactcc agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag 3000 ctggttgtgt tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg 3060 gaggcaaaga tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg 3120 cagagaacat caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc 3180 agactgtcat tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg 3240 aagggttcag agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa 3300 atgccagctc agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag 3360 aaacaaagct cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc 3420 tcactcaaca catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag 3480 ctggtgcgag aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg 3540 aaaccctcaa agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg 3600 actctgacat acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc 3660 aagggtttga caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga 3720 gaaccacact cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg 3780 atgctgccat gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca 3840 aggctcctga acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg 3900 agcttcaaga cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact 3960 ccaaacttgc tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag 4020 ttgacactgc tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg 4080 atggttatgg aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc 4140 cggcgcctgt taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg 4200 tgtccaacga gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga 4260 ctggatacga aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg 4320 acagcatcaa gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga 4380 aagatgttga tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg 4440 cagaaatcgc tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag 4500 cggctccagc tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg 4560 ttgtgatgga agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca 4620 tggagcttga gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag 4680 tccaagccat gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg 4740 tcggtgaggt tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg 4800 ccgctgccgc tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg 4860 tctcaaacga actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa 4920 ccggctatga gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag 4980 acagcatcaa acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga 5040 aggatgttga tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg 5100 ctgaaattgc tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg 5160 cggcacctgc tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg 5220 agacggttgt gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat 5280 ctgatatgga attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga 5340 gtgaagtcca agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa 5400 ggacagtggg tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg 5460 cgccagctgc cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac 5520 cggccgcgcc cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga 5580 tggaggtgct cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc 5640 ttgaaaccga gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag 5700 ctatgttgaa cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg 5760 aggtcgtgga cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag 5820 ccgcacctgc acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca 5880 atgagttgct ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt 5940 acgaaacgga catgattgag tcagacatgg aattggagac cgaacttgga atagattcca 6000 tcaagagagt ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg 6060 ttgatgcact cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga 6120 ttgctggagg ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac 6180 cagctgtcag caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg 6240 caaaaaccgg ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg 6300 gcattgacag catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg 6360 aggctaagga tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga 6420 tgaaggcgga gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg 6480 cagccgcggc accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa 6540 aggctgagac agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga 6600 ttgaatctga catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa 6660 tcctctctga ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca 6720 gaacaaggac ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg 6780 ctcctgcgcc agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg 6840 aactccttga aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg 6900 agactgacat gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca 6960 agcgtgtgga aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg 7020 atgctttgtc aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag 7080 ctggtggatc agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag 7140 ctgtgaagat tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa 7200 aggtggttga catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag 7260 ttcttgtcgt tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct 7320 gtgcagttgt cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca 7380 ttcgtcatgt tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg 7440 ctgagcaaag atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac 7500 cagctgagat actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca 7560 ctgctgtggc tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg 7620 gattcacctc tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg 7680 gactctgcaa gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg 7740 acattgctca agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt 7800 gtgctgacat cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca 7860 gagctgccaa gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc 7920 tggtttctgg aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga 7980 ttgctggagg caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat 8040 ggtgtgctgg catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga 8100 gggccttcag tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg 8160 gatctgttct tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg 8220 gcaaagcgat ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta 8280 gggatgcaga gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc 8340 tccgtgatcg tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca 8400 aagtcactgg gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg 8460 ttctcttcag ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg 8520 caaacgaagc tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca 8580 tctgctttgg tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag 8640 aaatgggagt tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt 8700 tgggatcaag tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg 8760 gaagtgacac gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg 8820 atcatgtcat acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg 8880 agacctgcct tggactcttc cctggctact ctctctgggc catagatgac gcacagctct 8940 tcaaaggtgt cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg 9000 ctccgagtgg gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg 9060 ttccagccta cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa 9120 ccatgcagcc tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca 9180 agactctctt tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga 9240 gccagcttgt tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg 9300 caactgacac tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg 9360 tctgggtgag gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc 9420 aacacagacc ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg 9480 gtggacatag ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca 9540 ttgatgttca agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa 9600 tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga 9660 tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg 9720 tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta 9780 ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa 9840 gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc 9900 tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg 9960 cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc 10020 ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta 10080 attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc 10140 acttactgat gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctagacc 10260 taggctcagc aactcatcca gtcaagttcg agtcagtgag ttgggcggcc gcagcttata 10320 gtaaagattt ttttgttaat aaaatgaaag ttaatattta tggatttatt tgaacatatt 10380 tacaagtttt tgttaataaa tttatttaca gaagtgttaa atttgaaaat gtgttaaatg 10440 agagttaata tttatggata taatattact ttatttaaaa ataaaatgaa tttatattaa 10500 aatatgattt ggtaaaagat attataaaga ggaacttgga tgagaaagac gacgatgggc 10560 aaaagttgca tggcacaccg cttttgttca gtaacatctg cactgtggcg cctttttcac 10620 actcaccatg catgctgcca cgtcagcttt cacactatga caccactggc catacacgtt 10680 gccacctcag ctttctcctc ttctcactat gacacgactg gccatgcatg catgcatgct 10740 gccacctcag ctcccgcctc ttcacccgtg tctttctctc atgtcgcagt gctgcgtgcc 10800 aacctcttct ctctataaat agagagaaga gaatgatggt taaactcaaa ccaaaacaag 10860 aaaacataca caaatagcaa aacggatcca caaccaaaaa tggctgcaag gaacgtgagt 10920 gcggctcatg agatgcatga cgagaagagg atagctgttg tgggcatggc tgttcagtat 10980 gctggatgca aaacgaaaga cgagttctgg gaagttctca tgaatggaaa agttgagagc 11040 aaagtgatca gcgacaaacg tcttggttca aattaccgtg ctgaacacta caaagctgaa 11100 aggagcaagt atgctgacac cttctgcaat gagacatatg gaactcttga tgagaacgaa 11160 atagacaatg agcatgaact ccttttgaac cttgccaaac aagccttggc tgaaacatct 11220 gtcaaagatt caacaagatg tggcatagtt tctggatgcc tcagcttccc gatggacaac 11280 ctccaaggtg aactcttgaa tgtgtaccag aaccatgttg agaagaaact tggtgccaga 11340 gttttcaaag atgcctctca ctggtcagaa agagaacagt ccaacaaacc agaggctggt 11400 gacagaagaa tcttcatgga ccctgcttcc tttgttgctg aggaactcaa tcttggcgct 11460 ctccactact ctgttgatgc tgcctgtgcc actgcgctct atgtgctcag actggcgcaa 11520 gatcacttgg tctctggtgc agctgatgtg atgctttgtg gtgcaacatg cctgccggag 11580 ccgtttttca tactctctgg gttctcaacc ttccaagcca tgcctgttgg aacgggtcag 11640 aatgtgtcca tgccgctgca caaagattct caaggattga cccctggtga aggaggttcc 11700 atcatggtcc tcaaacgttt ggatgacgca atcagagatg gagatcacat ctatggaaca 11760 cttttgggag ccaatgtcag caacagtggc actggtctgc cacttaaacc ccttttgccg 11820 tcagagaaaa agtgcctcat ggacacctac acacgtatca atgtgcatcc ccacaagatt 11880 cagtatgttg aatgccatgc aactgggact ccacaaggtg atcgtgtgga aattgatgct 11940 gtgaaggctt gctttgaagg caaagtccca cgttttggaa ccacaaaagg gaactttgga 12000 cacacattgg tggcagctgg gtttgctggc atgtgcaaag tgcttctctc catgaagcac 12060 gggatcattc cacccactcc gggaatagat gacgaaacga agatggaccc tctggttgtc 12120 tctggtgagg ccattccctg gccagaaacg aatggagaac cgaagcgtgc tggtctctca 12180 gcgtttggat ttggtgggac caatgctcat gctgtctttg aggaacatga cccaagcaat 12240 gctgcatgca ctggccatga ctccatttct gctctctctg cacgttgtgg tggtgagtca 12300 aacatgagaa tagcaatcac tgggatggat gccacttttg gtgctttgaa aggattggat 12360 gcttttgaaa gggccatcta cactggtgct catggtgcta taccactccc agaaaagaga 12420 tggagattcc tggggaaaga caaggacttc cttgatcttt gtggagtcaa agcaacaccg 12480 catggctgtt acattgaaga tgttgaagtt gatttccaga ggctgaggac tccaatgact 12540 cctgaagata tgctcttgcc tcaacagctt ctggctgtca ccacaattga tagggccata 12600 ttggactctg gcatgaagaa aggtggaaat gtggctgtct ttgtggggtt ggggaccgat 12660 cttgaactct accgtcaccg tgcaagggtt gctctcaagg agagagtgag gcctgaagcc 12720 agcaagaaac tcaatgacat gatgcagtac atcaatgatt gtgggacaag cacttcttac 12780 accagctaca taggcaactt ggttgcaaca agggtcagtt cacaatgggg cttcacggga 12840 ccttccttca caatcacaga aggcaacaat tctgtgtaca gatgtgctga acttggcaaa 12900 tacctcttgg aaacgggaga ggttgatggg gttgtggttg cgggtgttga tctctgtggg 12960 agtgctgaga acctttacgt caagtcaagg cgtttcaagg tgtctacatc tgacaccccg 13020 agagccagct ttgatgcagc tgctgatggt tactttgttg gagagggctg tggtgcattt 13080 gtgctcaagc gtgagacaag ctgcaccaag gatgacagaa tctatgcttg catggatgcc 13140 atagttcctg gaaatgttcc atctgcatgt ttgagagagg cattggatca agccagagtc 13200 aagcctggag acatcgagat gttggagctt tctgctgatt cagcgaggca tttgaaggac 13260 ccgtctgttc tcccaaagga actcactgct gaggaagaga ttggcggact tcagacgatt 13320 cttcgtgacg atgacaagct cccaaggaat gttgcaactg gaagtgtcaa ggccactgtt 13380 ggtgacactg gttatgcttc tggtgctgcc agtttgatta aggctgcgct ttgcatctac 13440 aaccgttacc tgcccagcaa tggagatgac tgggatgaac ctgcacctga agcaccctgg 13500 gacagcacac tctttgcgtg ccagacctca agagcttggt tgaagaaccc tggtgagaga 13560 cgttatgcgg ctgtttccgg agtcagtgaa accagaagtt gctacagtgt gctgctttct 13620 gaagcagagg gacactacga gagagagaac agaatcagtt tggatgagga agcacccaag 13680 ctcattgtgt tgagagcaga ctcccatgaa gagattcttg gaagattgga caagataagg 13740 gaaaggttct tgcagcccac gggtgctgca cccagagagt ctgaattgaa ggcgcaagcc 13800 cgtaggatct ttcttgaact ccttggtgag acccttgccc aagatgcagc ttcatctgga 13860 agtcagaagc cccttgctct cagcttggtc tccactccat ccaagctcca gagagaggtg 13920 gaacttgcgg caaaaggcat tccgagatgt ctcaagatga gacgtgattg gagttcccct 13980 gctggaagca gatatgcgcc tgagcctttg gccagtgaca gagttgcctt catgtatgga 14040 gaaggaagga gcccttacta tggcatcaca caagacattc ataggatttg gcctgaactt 14100 catgaggtca tcaatgagaa aacaaaccgt ctctgggctg aaggtgacag atgggtcatg 14160 ccaagggcca gcttcaagag tgagcttgag tcacaacagc aagagtttga cagaaacatg 14220 attgaaatgt tccgtcttgg aatcctcacc tcaattgcct tcacaaacct ggcgagagat 14280 gttctcaaca tcacgcccaa agcagccttt ggactcagtt tgggagagat ttccatgatc 14340 tttgctttct ccaagaaaaa cggtttgata agcgatcagt tgacaaagga tttgagagag 14400 tcagatgtct ggaacaaagc tctggctgtt gagttcaatg cgctcagaga agcctggggc 14460 attccacagt ctgtcccaaa agatgagttc tggcaaggtt acatagtgag gggaacgaaa 14520 caagacatag aagcagccat agcaccagac tcaaagtatg tgaggttgac catcatcaat 14580 gatgccaaca ctgctctcat cagtggaaag cctgatgcat gcaaagctgc gattgctcgt 14640 cttggtggca acatccctgc gcttccagtc actcaaggca tgtgtgggca ttgcccagag 14700 gtgggacctt acaccaagga catcgcaaag atccacgcca accttgagtt ccctgtggtt 14760 gatggtcttg atctctggac taccataaac cagaagaggc ttgtgccaag agccacgggt 14820 gccaaagatg aatgggctcc ttcatccttt ggggagtatg ctggacagct ctatgagaaa 14880 caagccaact tccctcagat agttgaaacc atctacaagc agaactatga tgtgtttgtt 14940 gaagttgggc caaacaatca cagaagcaca gcagtgagaa ccacgcttgg accacagaga 15000 aaccaccttg ctggtgccat agacaagcag aacgaggatg cttggacgac aatagtcaag 15060 ctcgtggctt cattgaaggc tcaccttgtt cctggagtga ccatcagtcc gctctaccac 15120 tccaagctcg ttgcagaggc tgaggcttgt tatgctgcgc tctgtaaagg tgagaaaccc 15180 aagaaaaaca agtttgtgag aaagattcag ttgaatggca gattcaacag caaggcagac 15240 cccatctcct cagcagacct tgcatccttc ccacctgcgg accctgcgat tgaagctgcc 15300 atctcttcaa ggatcatgaa gcctgtggct ccgaaattct atgcgcgtct caacattgat 15360 gagcaagatg agaccagaga tccgatcttg aacaaggaca atgcgccttc cagctccagc 15420 tcttccagca gttcctcttc cagcagttca tctccgtctc ctgctccttc agctccagtt 15480 cagaagaaag ctgccccagc tgcggagaca aaagctgttg ccagtgctga tgcacttcgt 15540 agtgccctgc ttgatcttga ctccatgctt gcgctcagct ctgcaagtgc ttctggcaac 15600 cttgttgaga ctgcgccatc tgatgccagt gtcattgtcc ctccgtgcaa cattgctgat 15660 cttggcagca gagctttcat gaaaacttat ggtgtttctg cgcctctgta caccggtgca 15720 atggccaagg gaattgcctc tgcggacttg gtgatcgcag ctggaaggca aggaatcctt 15780 gcgtcatttg gtgctggagg ccttccaatg caagttgtgc gtgagtccat tgaaaagatt 15840 caagctgccc tgcccaatgg accatatgct gtcaacctca tccattctcc ttttgacagc 15900 aaccttgaga agggaaatgt tgatctcttc ttggaaaagg gtgtcacttt tgttgaggct 15960 tcagccttca tgacgttgac accgcaagtt gtcagataca gagcggctgg gttgacgagg 16020 aatgcagatg ggtcagtcaa cataaggaac agaatcattg ggaaagtctc cagaactgag 16080 ctggctgaaa tgttcatgcg tcctgcacct gagcatcttt tgcagaagct cattgcttct 16140 ggggagatca atcaagaaca agctgagttg gccagacgtg ttcctgtggc tgatgacatt 16200 gcggttgaag ctgattctgg tggacacact gacaaccgtc caatccatgt cattctgcct 16260 ctgattatca acctgaggga taggcttcac agagagtgtg gatacccagc aaacctgagg 16320 gttcgtgttg gtgctggggg tggcattggg tgtccccaag ctgcgctggc aactttcaac 16380 atgggtgcct ctttcattgt cactggaaca gtgaaccaag ttgccaaaca atctgggacg 16440 tgtgacaatg tgaggaaaca gcttgcgaaa gcaacttact cagatgtttg catggcaccg 16500 gcagctgaca tgtttgagga aggtgtgaaa cttcaagttt tgaagaaagg aacaatgttc 16560 ccttcaagag ccaacaaact ctatgagttg ttctgcaagt atgattcttt tgagtccatg 16620 cctccagcag agcttgcgag ggttgagaaa agaatcttca gtcgtgcatt ggaagaggtc 16680 tgggatgaga ccaagaactt ctacatcaac cgtctccaca acccagagaa gatacagagg 16740 gctgagaggg accccaaact gaagatgtca ctgtgtttca gatggtatct gtccctggct 16800 tcaaggtggg ccaacactgg agcttctgac agagtcatgg actaccaagt ttggtgtggt 16860 cctgccattg gttcattcaa tgatttcatc aaaggaactt accttgatcc ggcagtggca 16920 aatgagtacc cgtgtgttgt gcagatcaac aaacagattc ttagaggagc gtgcttcttg 16980 aggcgtcttg aaatactgag aaatgcaagg ctttcagatg gtgcagctgc acttgtggcg 17040 agcattgatg acacatatgt ccctgctgag aagctgtgag tagttagctt aatcacttag 17100 gtcacccagt ccaagcccgg cctagcacga tgatcatgag tgttgctatt gatgtatgtt 17160 aatactatac acagtcatcg tgtgtgtttt ataaaactgt aagcttagga tgtttgaggc 17220 taatgtaatt agcactacac cgtaataaat gagaggttta ctttatgttt taataatgtt 17280 cttacccttg ctgctctcta aactgtaatg gcgtacggtt agctaaacta aagtaaaaat 17340 cgattcgctt gagtaaaatt agtgaccggg aaagatcatc gactactact gctcagagat 17400 gtaatcggta attaagactt caagctcaat aattaattag gagtaaatct ggcttcgaat 17460 ttttaaattg tcaacttcca aagaaggatt ggcaagactc aatctcaatt gcccaacaaa 17520 catatggctc tttacaaatt tggaatctct ttttgatggc aagaacgtcg taattcctct 17580 gtcgatctca tgtaattacc aaactcatct acttattact ttctttatat gcacttactg 17640 atgcggccgc ttaattaatc tgacctgacc actagacctg agtgagtaca gatgtgctac 17700 tagtgctagc ctcgaggtcg accaactttt ctatacaaag ttgtctagac ctaggactag 17760 gcactgagtg actcactgac ctgactgagt gactggcggc cgcagcttat agtaaagatt 17820 tttttgttaa taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt 17880 ttgttaataa atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat 17940 atttatggat ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt 18000 tggtaaaaga tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc 18060 atggcacacc gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat 18120 gcatgctgcc acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca 18180 gctttctcct cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca 18240 gctcccgcct cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc 18300 tctctataaa tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac 18360 acaaatagca aaacggatcc acaaccaaaa atggcgcttc gtgtcaagac caacaagaag 18420 ccatgttggg agatgaccaa ggaggaactg acctctggca aaactgaggt gttcaactat 18480 gaggaactcc tggagtttgc agagggtgac atagctaagg tttttggtcc ggagtttgct 18540 gtcatagaca agtaccccag aagggtgagg ctgcctgctc gtgaatacct gcttgtgaca 18600 agggtcacac tcatggatgc cgaggtcaac aactacagag ttggtgccag aatggtcact 18660 gagtatgatc ttccagtgaa tggcgaattg tctgagggag gtgattgtcc ctgggcagtt 18720 ctggttgaga gtggacagtg cgatttgatg ctgatatcat acatgggaat tgatttccag 18780 aaccaaggtg acagagttta ccgtctgctc aacaccacgt tgacattcta tggggttgcc 18840 catgagggag agacccttga gtatgacatc agagtgaccg gttttgccaa acgtttggat 18900 ggtggcatct ccatgttctt ctttgagtat gactgctatg tcaatgggag gcttctcatt 18960 gagatgagag atggctgtgc tggcttcttc acaaatgagg agcttgatgc gggaaagggt 19020 gtggtgttca caagagggga tttggctgcc agagctaaga ttccaaagca agatgtttct 19080 ccctatgctg tcgctccctg ccttcacaaa acaaaactca acgaaaagga gatgcagacc 19140 ttggttgaca aagattgggc ttccgttttt ggctcaaaga atggcatgcc ggagatcaac 19200 tacaaactct gcgcacgtaa gatgctcatg attgatagag tcaccagcat tgaccataag 19260 ggaggtgtgt acggtttggg tcagttggtt ggtgagaaaa tcttggagag ggaccattgg 19320 tacttcccat gccattttgt taaggatcaa gtcatggctg gatctcttgt cagcgacggt 19380 tgttcacaaa tgctcaagat gtacatgatc tggctcggtt tgcaccttac caccggacca 19440 tttgacttca gacccgtcaa cggacacccg aacaaagtta ggtgtagggg tcagatatct 19500 cctcataagg gaaagttggt ctacgtcatg gagatcaaag agatgggatt cgacgaggat 19560 aacgatccgt acgccattgc tgacgtcaac atcattgacg ttgatttcga aaagggtcaa 19620 gatttcagcc ttgatagaat cagcgactac ggaaaggggg atcttaacaa aaagatcgtc 19680 gtggacttca agggcattgc tctcaagatg cagaagagat ccacgaacaa gaacccctcc 19740 aaagttcagc ccgtttttgc taacggtgca gccactgtgg gaccggaagc gtccaaggcc 19800 agctctggtg ccagtgctag tgcttctgct gcccctgcga agcctgcttt ctcagcagat 19860 gttcttgcgc caaagccagt tgcacttcct gaacatatcc tcaagggtga cgctctcgct 19920 ccgaaagaaa tgtcctggca tcccatgtca aaacttcctg gcaacccgac gccgttcttc 19980 tcccctagtt cttaccctcc gagggcaatt tgcttcatcc ctttccctgg caacccactt 20040 gacaacaatt gcaaagctgg agaaatgcct ctgaactggt acaacatgtc agagttcatg 20100 tgtggaaaag ttagcaactg cttgggacca gagttcgcaa gatttgataa gtcaaatacg 20160 agcagaagcc ctgcttttga tttggctctt gtgactagag ttgttgaagt cacaaacatg 20220 gaacatggca agttcctgaa tgttgattgc aatcccagca aaggcacaat ggtgggggag 20280 tttgactgtc ctcaagatgc gtggttcttt gatggttctt gcaatgatgg gcacatgcct 20340 tattccatca tcatggaaat cggacttcag acatccggtg ttctcaccag tgtgttgaaa 20400 gcaccactga cgatggacaa agatgacatt ctctttagga accttgatgc aagtgctgaa 20460 atggtgaggc cagatgtgga tgtgaggggg aaaaccataa ggaatgtgac caagtgcact 20520 ggatatgcaa tgttgggaaa gatggggatt cacagattca cgtttgagtt gtctgttgat 20580 ggcgtggttt tctacaaagg aagcacttcc tttggatggt tcactccaga ggtgtttgct 20640 cagcaagctg gacttgacaa tgggaaaaag acggagcctt ggtgcaaaac taacaacacg 20700 agtgtgagaa gagttgaaat cgcatcagcc aaaggcaaag aacagctgac tgagaaactt 20760 ccagatgcaa ctaatgctca agttctcaga aggtcagaac agtgtgagta tttggattac 20820 ctcaacattg ctcctgattc tgggctgcat gggaaaggat atgcgcatgg acacaaagat 20880 gttaacccac aagactggtt cttctcttgt cacttttggt ttgatcctgt gatgcctgga 20940 tctttgggaa ttgagtcaat gttccagctc attgaggcat ttgcggtgga ccagaacata 21000 ccgggagaat acaatgtgag caatccgacc tttgcccatg cccctggaaa aacggcgtgg 21060 aagtaccgtg gtcaattgac accaaagaac cgtgcgatgg actgtgaggt gcacatagtt 21120 tcaatcacag cctctcctga aaatggaggc tacgttgaca tagtggcaga tggagcactt 21180 tgggttgatg gtctccgtgt gtatgaagcc aaagaactca gagttcgtgt tgtcagtgca 21240 aaacctcaag ccattccgga tgttcaacaa cagccaccca gcgcaaaagc ggaccctggg 21300 aaaactggag ttgcactcag tccgactcag ttgagagatg tcctgcttga agtggacaat 21360 ccattgtatc ttggtgttga aaactccaat ttggtgcagt ttgaatccaa acctgcaact 21420 tcttcaagga ttgtcagtat caaaccatgc tccataagtg atcttggtga caagtccttc 21480 atggaaacgt acaatgtgtc agcacctctg tacactggag caatggccaa gggaattgcg 21540 tctgcggacc ttgtcattgc tgctgggaag aggaagatat tgggttcttt tggagcgggt 21600 gggcttccaa tgcaccatgt gagggcagct ttggagaaga tacaagcggc tctgcctcaa 21660 ggaccatatg ctgtgaacct cattcattca ccttttgaca gcaacttgga aaaggggaat 21720 gttgatctct tcctcgaaaa gggcgttact gtggtggagg cttctgcatt catgaccctc 21780 actccccaag ttgttcgtta ccgtgcagcc ggactctcaa ggaacgctga tgggagtgtc 21840 aacatcagaa accgtatcat tggaaaagtg tccagaacag aactcgccga aatgttcatc 21900 agaccggctc cggaacacct ccttgagaaa ctcatagctt ctggtgagat aacacaagaa 21960 caagccgaac ttgcccgtcg tgttcctgtc gcagacgaca tcgctgttga agctgattct 22020 ggtggtcaca ctgacaaccg tcccatccac gtcatcttgc ctttgatcat caaccttagg 22080 aacagactgc acagagagtg tggttatcct gcgcacctta gagtcagagt tggagctgga 22140 ggcggtgttg gctgcccaca agctgctgcg gctgccctca caatgggagc tgccttcata 22200 gtgactggga ctgtcaatca agttgctaaa cagtccggga catgtgacaa cgtgagaaaa 22260 caactcagtc aagccaccta cagtgacatt tgcatggctc cagcagctga tatgtttgag 22320 gaaggtgtga aactccaagt tctcaagaag ggaacaatgt tcccatcaag agctaacaaa 22380 ttgtacgaac tcttttgcaa gtacgactct ttcgattcaa tgcctcctgc tgaacttgaa 22440 aggatcgaga agaggatctt caagagggca cttcaagagg tctgggaaga gaccaaggac 22500 ttttacatca atggtttgaa gaatccagag aagatacaga gagcggaaca tgaccccaaa 22560 ctcaagatgt ctttgtgttt cagatggtat cttggtcttg catcacgttg ggccaacatg 22620 ggtgctcctg atcgtgtcat ggactatcaa gtctggtgtg gtccagccat tggagccttc 22680 aatgacttca tcaaaggaac atatcttgat cctgctgtct ctaatgagta cccatgtgtt 22740 gtccagatca accttcagat tttgcgtggt gcatgttacc tgaggcgtct taatgctctg 22800 agaaatgatc cgaggattga tcttgagact gaagatgctg cctttgtcta tgagccaacg 22860 aatgcgcttt gagtagttag cttaatcact taggtcaccc agtccaagcc cggcctagca 22920 cgatgatcat gagtgttgct attgatgtat gttaatacta tacacagtca tcgtgtgtgt 22980 tttataaaac tgtaagctta ggatgtttga ggctaatgta attagcacta caccgtaata 23040 aatgagaggt ttactttatg ttttaataat gttcttaccc ttgctgctct ctaaactgta 23100 atggcgtacg gttagctaaa ctaaagtaaa aatcgattcg cttgagtaaa attagtgacc 23160 gggaaagatc atcgactact actgctcaga gatgtaatcg gtaattaaga cttcaagctc 23220 aataattaat taggagtaaa tctggcttcg aatttttaaa ttgtcaactt ccaaagaagg 23280 attggcaaga ctcaatctca attgcccaac aaacatatgg ctctttacaa atttggaatc 23340 tctttttgat ggcaagaacg tcgtaattcc tctgtcgatc tcatgtaatt accaaactca 23400 tctacttatt actttcttta tatgcactta ctgatgcggc cgcttaatta atcacgagtg 23460 gatgactgtc acgactgact catgactgac tactagtgct agcctcgagg tcgacacaac 23520 tttgtataat aaagttgtct agacctaggc gagtcatgag tcgtgacatg acgtcatgct 23580 catcagactg cggccgcagc ttatagtaaa gatttttttg ttaataaaat gaaagttaat 23640 atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat ttacagaagt 23700 gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata ttactttatt 23760 taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat aaagaggaac 23820 ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt gttcagtaac 23880 atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca gctttcacac 23940 tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc actatgacac 24000 gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac ccgtgtcttt 24060 ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga gaagagaatg 24120 atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg atccacaacc 24180 aaaaatggct cccactcccg acgccaccgc gcctctgaac aagccgagcg actatgccgt 24240 ctaccacgag gaagacggcc ccttctggac cgccgattcc agcggcgtct cgcgcgtgaa 24300 ctttagcgag accggcgtgg gatccgaggg cgtcatccct gcgctcacgc tcatcgacgt 24360 cttcgagagg gccgtcaagc gcggcggaaa caggatcgcc ttccgcacgg aaaacatgcc 24420 cacgctccgc cgcggcgaag aggccccgga cgcgctgccg ctcaaggact ggaagtcctg 24480 gtcctggaaa cagtacaagg ccgacgtcca ccgcatcgcc agggctctca tggacctcgg 24540 cgttgagcag cacgacgccg tctcgatttt tggctttaac tcgcccgaat ggtttctcag 24600 tgccgtcggc gccgtgcacg caggtgccaa gattgccggt atttacccct cagacacgcc 24660 cgcccaggtc cagtacaagg ccttccacag tgacaccgct gttgccgttg tcgaaaacga 24720 gcagtgcttc aagaagttcg ccgaggtcgt cgaggacctt ccttacctca aggccattgt 24780 ttgctgggac tatgaagcca cagacatcac gcgcgaggac ggctccgtcg tcgaggtcct 24840 cacctttgcc gagttcctca agcgcggcga caccgtcgag gcggccgccc ttgacgagcg 24900 catctccaag atcgagccca ccatgtgcgc tgcccttatt tacaccagcg gtactaccgg 24960 ccgccccaag gccgttatga tttcgcacga caaccttgtt ttcgaggcca gcgccgtcgt 25020 ccccaacctc ggaggagcct gtacgaccac tgctgaggag cgcattctct cgtacctgcc 25080 tctctcgcac gtcgctggta tgatggttga tattattgcc cccatcattg ccaccgcctt 25140 ccacaagggc cgcatctgcg tctgctttgc tcgcccgtac gatttgcgca ccggcacgct 25200 gggccagcgc ctcaacgccg tggagcccac catcttcctt ggcgtgcccc gtgtgtggga 25260 aaagattcag gaaaagctca tggccgtcgg tgccaaaacc accggcctca agaagaagct 25320 ctctacggcc gccaagaagc gtggtcttga attccaggag gagcagcaaa tcggccgctc 25380 cggtgccaac cctggctttg gcccccttgg catctacaag aagctcctcg gcctcatcaa 25440 gggcaagctg ggcctcacca agtgcaagtt tgcctttgct ggtgccgcgc ccatgacccg 25500 tgagaccctt cagtactttg gcgcgctgaa catcaacatt aacgaggtct acggcatgtc 25560 agagtgctcc ggtgccgcca cctggtccac ggacaaggcc cacgagtggg gcactgttgg 25620 ctacgagatg cccagttgcg aggtccgcgt cttcaagatt gccgaggacg gtaccaagac 25680 cgagtgcccg cgcgccgccg acattatgca tgctaccgag gaggagcagg gcgaagtttg 25740 cttccgcggc cgtaacatca tgatgggcta ccttgccaac cccaagcttg gcgacgacca 25800 cgttgccgag atcgaggaga agaacgctgc cgctatcgac tccgagggct ggctccacag 25860 tggtgataag ggcgccattt ctacccgcgg catgctcaag atcacgggcc gctacaagga 25920 gctcatcatc ggcgccggtg gcgagaacgt ggcgcccgtc cctattgagg acgccatcaa 25980 ggcgcgcatg ccttttgttt ccaacgccat gatggtcgga gataagcgca agttcatggc 26040 tgtcctcctt accctcaaga cggttggcgc cacgggcgag cttcccggta cgaacaagct 26100 catgggcgct gccgccgact acggtgagac catcgaggac gcctgcgaca acgaggcgct 26160 cattgaggag atcacgcagc agctcaagga gatcggtgat gatggcgatg tcacgccctc 26220 gaacgcggct cgcatccaaa agttcaccat tctcccgctc gacttttccg tctccacgga 26280 cgagctcacg gccacgctca agctcaagcg ctccgtggtc gcagacaagt acgaagacat 26340 catcgaggcc ttttacgagt ccaagagcgt ttttgtgccg tactcgaccg ttggcgccta 26400 cgccacgggc ggcccggtcg acgactccgt tgtcgatggc tcattcaagg gcgactttag 26460 catgattggc gacgatgatc cggatcttca aaacgtcgat gtcctcgagg cgattgacga 26520 ggacaattaa gtagttagct taatcactta ggtcacccag tccaagcccg gcctagcacg 26580 atgatcatga gtgttgctat tgatgtatgt taatactata cacagtcatc gtgtgtgttt 26640 tataaaactg taagcttagg atgtttgagg ctaatgtaat tagcactaca ccgtaataaa 26700 tgagaggttt actttatgtt ttaataatgt tcttaccctt gctgctctct aaactgtaat 26760 ggcgtacggt tagctaaact aaagtaaaaa tcgattcgct tgagtaaaat tagtgaccgg 26820 gaaagatcat cgactactac tgctcagaga tgtaatcggt aattaagact tcaagctcaa 26880 taattaatta ggagtaaatc tggcttcgaa tttttaaatt gtcaacttcc aaagaaggat 26940 tggcaagact caatctcaat tgcccaacaa acatatggct ctttacaaat ttggaatctc 27000 tttttgatgg caagaacgtc gtaattcctc tgtcgatctc atgtaattac caaactcatc 27060 tacttattac tttctttata tgcacttact gatgcggccg cttaattaat ctgatcatgg 27120 atgctacgtc acggcagtac aggactatcc ctaggagctg tgctgggtaa ctcacatgag 27180 tcactggtca catcagcggc cgcagcttat agtaaagatt tttttgttaa taaaatgaaa 27240 gttaatattt atggatttat ttgaacatat ttacaagttt ttgttaataa atttatttac 27300 agaagtgtta aatttgaaaa tgtgttaaat gagagttaat atttatggat ataatattac 27360 tttatttaaa aataaaatga atttatatta aaatatgatt tggtaaaaga tattataaag 27420 aggaacttgg atgagaaaga cgacgatggg caaaagttgc atggcacacc gcttttgttc 27480 agtaacatct gcactgtggc gcctttttca cactcaccat gcatgctgcc acgtcagctt 27540 tcacactatg acaccactgg ccatacacgt tgccacctca gctttctcct cttctcacta 27600 tgacacgact ggccatgcat gcatgcatgc tgccacctca gctcccgcct cttcacccgt 27660 gtctttctct catgtcgcag tgctgcgtgc caacctcttc tctctataaa tagagagaag 27720 agaatgatgg ttaaactcaa accaaaacaa gaaaacatac acaaatagca aaacggatcc 27780 acaaccaaaa atgcttcagc acacttggct tccgaagcct cccaatctga ccctcttgtc 27840 agatgaggtt catctctgga ggattcctct tgaccagcct gagtcacaac ttcaagacct 27900 tgctgccacc ctgagcagtg atgaattggc gagggcaaac agattctact ttccagaaca 27960 cagaaggcgt ttcactgctg ggagaggcat cctcagatcc atcttgggtg gatacttggg 28020 agtggaaccg ggtcaagtca agtttgatta tgagtcccgt gggaaaccga tccttggtga 28080 cagatttgct gagagtggac tcctgttcaa cttgtctcac agccagaacc ttgccttgtg 28140 tgctgtcaac tacacgcgtc aaataggcat tgatcttgaa tatctgcgtc caacatctga 28200 cttggagtct cttgcaaaga ggttctttct cccaagagaa tatgaactct tgaggtcact 28260 ccctgatgag cagaaacaga agattttctt tcgttactgg acttgcaaag aggcttatct 28320 caaagcaacg ggagatggaa tagccaaact tgaagagatc gagatagcac tcaccccaac 28380 agaacctgcc aagctccaaa cagctcctgc gtggtctctg ttggagcttg tgccagatga 28440 caattgtgtt gcagctgtgg ctgttgcggg ttttggttgg cagcccaagt tctggcatta 28500 ctgagtagtt agcttaatca cttaggtcac ccagtccaag cccggcctag cacgatgatc 28560 atgagtgttg ctattgatgt atgttaatac tatacacagt catcgtgtgt gttttataaa 28620 actgtaagct taggatgttt gaggctaatg taattagcac tacaccgtaa taaatgagag 28680 gtttacttta tgttttaata atgttcttac ccttgctgct ctctaaactg taatggcgta 28740 cggttagcta aactaaagta aaaatcgatt cgcttgagta aaattagtga ccgggaaaga 28800 tcatcgacta ctactgctca gagatgtaat cggtaattaa gacttcaagc tcaataatta 28860 attaggagta aatctggctt cgaattttta aattgtcaac ttccaaagaa ggattggcaa 28920 gactcaatct caattgccca acaaacatat ggctctttac aaatttggaa tctctttttg 28980 atggcaagaa cgtcgtaatt cctctgtcga tctcatgtaa ttaccaaact catctactta 29040 ttactttctt tatatgcact tactgatgcg gccgcttaat taactactgt cactgaggcc 29100 gtagacgagt acggactgat ctaactagtg ctagcctcga ggtcgacacc cagctttctt 29160 gtacaaagtg gttgcggccg cttaattaaa tttaaattca attaatgcaa tcttgatttt 29220 caacaacgaa ggtaatggcg taaaagaaaa aatgtatgtt attgtattga tctttcatga 29280 tgttgaagcg tgccataata tgatgatgta taattaaaat attaactgtc gcattttatt 29340 gaaatggcac tgttatttca accatatctt tgattctgtt acatgacacg actgcaagaa 29400 gtaaataata gacgccgttg ttaaagaatt gctatcatat gtgcctaact agagggaatt 29460 tgagcgtcag acctaatcaa atattacaaa atatctcact ctgtcgccag caatggtgta 29520 atcagcgcag acaaatggcg taaagatcgc ggaaaaacct ccccgagtgg catgatagct 29580 gcctctgtat tgctgattta gtcagcctta tttgacttaa gggtgccctc gttagtgaca 29640 aattgctttc aaggagacag ccatgcccca cactttgttg aaaaacaaat tgcctttggg 29700 gagacggtaa agccagttgc tcttcaataa ggaatgtcga ggaggcaatg taaccgcctc 29760 tggtagtaca cttctctaat ccaaaaatca atttgtattc aagataccgc aaaaaactta 29820 tggtttaaac cctgcaggac tagtccagaa ggtaattatc caagatgtag catcaagaat 29880 ccaatgttta cgggaaaaac tatggaagta ttatgtaagc tcagcaagaa gcagatcaat 29940 atgcggcaca tatgcaacct atgttcaaaa atgaagaatg tacagataca agatcctata 30000 ctgccagaat acgaagaaga atacgtagaa attgaaaaag aagaaccagg cgaagaaaag 30060 aatcttgaag acgtaagcac tgacgacaac aatgaaaaga agaagataag gtcggtgatt 30120 gtgaaagaga catagaggac acatgtaagg tggaaaatgt aagggcggaa agtaacctta 30180 tcacaaagga atcttatccc ccactactta tccttttata tttttccgtg tcatttttgc 30240 ccttgagttt tcctatataa ggaaccaagt tcggcatttg tgaaaacaag aaaaaatttg 30300 gtgtaagcta ttttctttga agtactgagg atacaacttc agagaaattt gtaagtttgt 30360 aggtaccaga tctggatccc aaaccatgtc tccggagagg agaccagttg agattaggcc 30420 agctacagca gctgatatgg ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc 30480 tacagtgaac tttaggacag agccacaaac accacaagag tggattgatg atctagagag 30540 gttgcaagat agataccctt ggttggttgc tgaggttgag ggtgttgtgg ctggtattgc 30600 ttacgctggg ccctggaagg ctaggaacgc ttacgattgg acagttgaga gtactgttta 30660 cgtgtcacat aggcatcaaa ggttgggcct aggatctaca ttgtacacac atttgcttaa 30720 gtctatggag gcgcaaggtt ttaagtctgt ggttgctgtt ataggccttc caaacgatcc 30780 atctgttagg ttgcatgagg ctttgggata cacagcccgg ggtacattgc gcgcagctgg 30840 atacaagcat ggtggatggc atgatgttgg tttttggcaa agggattttg agttgccagc 30900 tcctccaagg ccagttaggc cagttaccca aatctgagta gttagcttaa tcacctagag 30960 ctcgatcggc ggcaatagct tcttagcgcc atcccgggtt gatcctatct gtgttgaaat 31020 agttgcggtg ggcaaggctc tctttcagaa agacaggcgg ccaaaggaac ccaaggtgag 31080 gtgggctatg gctctcagtt ccttgtggaa gcgcttggtc taaggtgcag aggtgttagc 31140 gggatgaagc aaaagtgtcc gattgtaaca agatatgttg atcctacgta aggatattaa 31200 agtatgtatt catcactaat ataatcagtg tattccaata tgtactacga tttccaatgt 31260 ctttattgtc gccgtatgta atcggcgtca caaaataatc cccggtgact ttcttttaat 31320 ccaggatgaa ataatatgtt attataattt ttgcgatttg gtccgttata ggaattgaag 31380 tgtgcttgag gtcggtcgcc accactccca tttcataatt ttacatgtat ttgaaaaata 31440 aaaatttatg gtattcaatt taaacacgta tacttgtaaa gaatgatatc ttgaaagaaa 31500 tatagtttaa atatttattg ataaaataac aagtcaggta ttatagtcca agcaaaaaca 31560 taaatttatt gatgcaagtt taaattcaga aatatttcaa taactgatta tatcagctgg 31620 tacattgccg tagatgaaag actgagtgcg atattatggt gtaatacata ggaattcgtt 31680 taaacgatct gcgtctaatt ttcggtccaa cttgcacagg aaagacgtcg accgcggtag 31740 ctcttgccca gcagactggg cttccagtcc tttcgctcga tcgggtccaa tgttgtcctc 31800 agctgtgaac cggaagcgga cgaccaacag tggaagaact gaaaggaacg agccgtctat 31860 accttgatga tcggcctctg gtgaagggta tcatcgcagc caagcaagct catgaaaggc 31920 tgatggggga ggtgtataat tatgaggccc acggcgggct tattctttag ggaggatcta 31980 tctcgttgct caagtgcatg gcgcaaagca gttattggag tgcggatttt cgttggcata 32040 ttattcgcca cgagttagca gacgaagaga ccttcatgaa cgtggccaag gccagagtta 32100 agcagatgtt acgccctgct gcaggccttt ctattatcca atagttggtt gatctttgga 32160 aagagcctcg gctgaggccc atactgaaag agatcgatgg atatcgatat gccatgttgt 32220 ttgctagcca gaaccagatc acatccgata tgctattgca gcttgacgca gatatggagg 32280 ataagttgat tcatgggatc gctcaggagt agctcatcca tgcacgccga caagaacaga 32340 aattccgtcg agttaacgca gccgcttacg acggattcga aggtcatcca ttcggaatgt 32400 attagtttgc accagctccg cgtcacacct gtcttcattt gaataagatg ttagcaattg 32460 tttttagctt tgtcttgttg tggcagggcg gcaagtgctt cagacatcat tctgttttca 32520 aattttatgc tggagaacag cttcttaatt cctttggaaa taatagactg cgtcttaaaa 32580 ttcagatgtc tggatataga tatgattgta aaataaccta tttaagtgtc atttagaaca 32640 taagttttat gaatgttctt ccattttcgt catcgaacga ataagagtaa atacaccttt 32700 tttaacatta caaataagtt cttatacgtt gtttatacac cgggaatcat ttccattatt 32760 ttcgcgcaaa agtcacggat attcgtgaaa gcgacataaa ctgcgaaatt tgcggggagt 32820 gtcttgagtt tgcctcgagg ctagcgcatg cacatagaca cacacatcat ctcattgatg 32880 cttggtaata attgtcatta gattgttttt atgcatagat gcactcgaaa tcagccaatt 32940 ttagacaagt atcaaacgga tgtgacttca gtacattaaa aacgtccgca atgtgttatt 33000 aagttgtcta agcgtcaatt tgatttacaa ttgaatatat cctgccccag ccagccaaca 33060 gctcgattta caattgaata tatcctgccg gccggcccac gcgtgtcgag gaattctgat 33120 ctggccccca tttggacgtg aatgtagaca cgtcgaaata aagatttccg aattagaata 33180 atttgtttat tgctttcgcc tataaatacg acggatcgta atttgtcgtt ttatcaaaat 33240 gtactttcat tttataataa cgctgcggac atctacattt ttgaattgaa aaaaaattgg 33300 taattactct ttctttttct ccatattgac catcatactc attgctgatc catgtagatt 33360 tcccggacat gaagccattt acaattgaat atatcctgcc gccgctgccg ctttgcaccc 33420 ggtggagctt gcatgttggt ttctacgcag aactgagccg gttaggcaga taatttccat 33480 tgagaactga gccatgtgca ccttcccccc aacacggtga gcgacggggc aacggagtga 33540 tccacatggg acttttaaac atcatccgtc ggatggcgtt gcgagagaag cagtcgatcc 33600 gtgagatcag ccgacgcacc gggcaggcgc gcaacacgat cgcaaagtat ttgaacgcag 33660 gtacaatcga gccgacgttc acgcggaacg accaagcaag cttggctgcc atttttgggg 33720 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg 33780 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg 33840 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca 33900 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg 33960 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt 34020 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg 34080 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt 34140 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca 34200 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg 34260 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca 34320 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg 34380 gcgagggcaa ccagcccggt gagcgtcgga aagggtcgac ggatcttttc cgctgcataa 34440 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat 34500 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg 34560 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat 34620 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc 34680 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca 34740 cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg 34800 gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc 34860 gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg 34920 ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc 34980 acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg 35040 atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg 35100 ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga 35160 gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt 35220 ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg 35280 gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg 35340 gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt 35400 ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac 35460 cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt 35520 gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct 35580 cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac 35640 gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca 35700 ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga 35760 tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct 35820 gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca 35880 cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga 35940 actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac 36000 cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac 36060 gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg 36120 cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa 36180 gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga 36240 ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat 36300 gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct 36360 catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg 36420 cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca 36480 ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag ccagcgcttt 36540 actggcattt caggaacaag cgggcactgc tcgacgcact tgcttcgctc agtatcgctc 36600 gggacgcacg gcgcgctcta cgaactgccg ataaacagag gattaaaatt gacaattgtg 36660 attaaggctc agattcgacg gcttggagcg gccgacgtgc aggatttccg cgagatccga 36720 ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg tttacgagca cgaggagaaa 36780 aagcccatgg aggcgttcgc tgaacggttg cgagatgccg tggcattcgg cgcctacatc 36840 gacggcgaga tcattgggct gtcggtcttc aaacaggagg acggccccaa ggacgctcac 36900 aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc gaggccgagg ggtcgccggt 36960 atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca 37020 acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg 37080 agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct 37140 gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg 37200 atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca 37260 aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg 37320 ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc 37380 tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca 37440 atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg 37500 ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc 37560 ttactgggct ttctcagccc ccgagcgctt agtgggaatt tgtacccctt atcgaaccgg 37620 gagcacagga tgacgcctaa caattcattc aagccgacac cgcttcgcgg cgcggcttaa 37680 ttcaggagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag 37740 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg 37800 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga 37860 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg 37920 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg 37980 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca 38040 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc 38100 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg 38160 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact 38220 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt 38280 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc 38340 gcctgccggc ccagtatcag cccgtcatac ttgaagctag gcaggcttat cttggacaag 38400 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg 38460 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg 38520 cggcgcggct taactcaagc gttagagagc tggggaagac tatgcgcgat ctgttgaagg 38580 tggttctaag cctcgtcttg cgatggcatt tcgatccatt cccattccgc gctcaagatg 38640 gcttcccctc ggcagttcat cagggctaaa tcaatctagc cgacttgtcc ggtgaaatgg 38700 gctgcactcc aacagaaaca atcaaacaaa catacacagc gacttattca cacgagctca 38760 <210> 46 <211> 34862 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9166 <400> 46 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcgaa ttcggaaatg ggccaagtga aatggaaata 300 gagcttcaat ccatttagtc ccactcaaaa tggtgctcga attatattta gttacgttcg 360 aatcagacaa ccaagtattt ggttaataaa aaccactcgc aacaaaggaa aaacaccaag 420 cgcgtgcgtc caacatccga cggaaggggg gtaatgtggt ccgaaaacct tacaaaaatc 480 tgacgtcatc tacccccgaa aacgttgaat cgtcaacggg ggtagttttc gaattatctt 540 ttttttaggg gcagttttat taatttgctc tagaaatttt atgattttaa ttaaaaaaag 600 aaaaagaata tttgtatatt tattttttat actctttttt tgtccaacta tttctcttat 660 tttggcaact ttaactagac tagtaactta tgtcaatgtg tatggatgca tgagagtgag 720 tatacacatg tctaaatgca tgccttatga aagcaacgca ccacaaaacg aagacccctt 780 tacaaataca tctcatccct tagtaccctc ttactactgt cccgacacaa actcaaaaca 840 aggatccaca accaaaaatg gcagcgaggc tgcaagagca gaaaggaggt gagatggaca 900 cgaggattgc gatcataggc atgagcgcca tacttccttg tggaaccacg gtgagagagt 960 catgggagac cataagggct ggcattgatt gcctgtctga tcttcctgag gatcgtgttg 1020 atgtgactgc gtactttgac ccagtcaaga ccacaaagga caaaatctac tgcaagagag 1080 gaggtttcat tcctgagtat gactttgatg cgcgtgagtt tggtctcaac atgttccaga 1140 tggaagattc tgatgcgaat cagaccatct cacttctcaa ggtcaaagaa gcacttcaag 1200 atgctgggat tgatgcactt ggaaaggaga agaaaaacat aggatgcgtg cttggcattg 1260 gaggtggaca gaagagttca catgagttct acagcagact caactatgtg gttgtggaga 1320 aagtcttgag aaagatgggg atgccagagg aagatgtcaa agtggctgtg gagaagtaca 1380 aagcgaactt cccagaatgg agacttgact ctttccctgg attccttggg aatgtcactg 1440 ctggcagatg caccaacaca ttcaacttgg atggcatgaa ctgcgttgtg gatgctgcat 1500 gtgcaagctc cttgatagca gtcaaggttg cgattgatga actgctctat ggtgactgcg 1560 acatgatggt cactggtgca acctgcacag acaactccat tgggatgtac atggctttct 1620 caaagacacc tgtgttcagc acggacccaa gtgtgagagc gtatgatgag aaaacgaaag 1680 ggatgctcat tggtgaggga tcagccatgt tggtgctcaa acgttatgca gatgcagtga 1740 gggatggtga tgagattcat gctgtgattc gtggttgtgc aagctcaagt gatggcaaag 1800 cagctggcat ctacactccc acaatcagtg gtcaagagga agccttgaga cgtgcataca 1860 atcgtgcctg cgttgaccct gccactgtca ctttggttga gggtcatggg actggcactc 1920 cagttggaga taggattgag ttgactgcgt tgaggaactt gtttgacaaa gcgtatggtg 1980 aagggaacac agagaaagtt gcagttggaa gtatcaagtc cagtattggt catttgaagg 2040 ctgttgctgg tcttgctgga atgatcaagg tcatcatggc tctcaaacac aagactttgc 2100 ctggaacgat caatgtggac aatccaccca atctctatga caacactccc atcaatgagt 2160 catccctcta catcaacacg atgaacagac cctggttccc tccacctggt gttccgagac 2220 gtgctggaat atccagcttt ggctttggag gtgccaacta ccatgcagtg ttggaagagg 2280 ctgaaccaga gcacacgaca gcgtacagac tcaacaaacg tccacaaccg gtgttgatga 2340 tggctgcaac tcctgctgcg ttgcagagcc tttgtgaagc tcagctgaaa gagtttgaag 2400 ctgcgatcaa ggagaatgag acagtcaaga atactgccta catcaagtgt gtcaagtttg 2460 gagaacagtt caagttccct gggagcatac ctgcgaccaa tgcgagactg ggattcttgg 2520 tcaaagatgc ggaggatgcg tgctccacgc ttcgtgctat atgtgctcag tttgccaaag 2580 atgtgaccaa agaagcgtgg aggcttccga gagaaggagt ttctttccgt gccaaaggca 2640 ttgccaccaa tggtgctgtt gctgcactct tctctggaca aggtgctcag tacacacaca 2700 tgttcagtga agttgcgatg aactggcctc agttcagaca gtccattgct gcaatggacg 2760 ctgcacagtc caaggttgct ggaagtgaca aagactttga aagagtctct caagtcctgt 2820 acccaaggaa accgtacgaa cgtgagccag aacaagatca caagaaaatc tcactcacag 2880 catacagcca gccgtcaacc ctggcatgtg ctcttggtgc ctttgagatt ttcaaggaag 2940 ctggattcac tccagacttt gcagctggac acagcttggg tgagtttgct gcgctctatg 3000 cagctggttg tgttgacaga gatgagttgt ttgaacttgt ttgcagaagg gcaaggatca 3060 tgggaggcaa agatgcacct gccacaccca aaggttgcat ggcagctgtc ataggaccca 3120 acgcagagaa catcaaggtt caagctgcca acgtctggct tggaaactca aactctccgt 3180 ctcagactgt cattactggg tctgttgaag ggattcaagc tgaaagtgcc agattgcaga 3240 aggaagggtt cagagttgtg cctcttgctt gtgagtctgc cttccattct ccacagatgg 3300 aaaatgccag ctcagctttc aaagatgtca tatccaaggt ttccttccgt acacccaaag 3360 cagaaacaaa gctcttcagc aatgtttctg gagagaccta tccgacggat gccagagaga 3420 tgctcactca acacatgacc tcatctgtga agttcctgac ccaagtgagg aacatgcacc 3480 aagctggtgc gagaatcttt gttgagtttg gaccgaaaca agttctcagc aaacttgttt 3540 ctgaaaccct caaagatgac ccgtctgttg tcacagtgtc tgtgaaccca gcttctggca 3600 cggactctga catacagttg agggatgctg cagttcagct ggttgtggct ggtgtcaatc 3660 ttcaagggtt tgacaagtgg gatgctcctg atgccacgag gatgcaagcc atcaagaaaa 3720 agagaaccac actcagactt tcagctgcca cctatgtcag tgacaagacc aagaaagtga 3780 gggatgctgc catgaacgac ggaagatgtg tcacatacct caagggtgct gcaccactca 3840 tcaaggctcc tgaacctgtg gttgacgaag ctgccaagag ggaggctgaa aggcttcaga 3900 aggagcttca agacgctcag aggcagcttg acgacgccaa acgtgcagct gcagaagcca 3960 actccaaact tgctgctgcc aaggaggaag ccaagactgc tgcagcttca gccaagcctg 4020 cagttgacac tgctgttgtg gagaaacatc gtgccatcct caagtccatg ctggctgagc 4080 tggatggtta tggaagtgtt gatgcttctt cccttcagca acagcaacag caacagactg 4140 ctccggcgcc tgttaaagcc gctgcccctg ccgctcccgt tgcaagtgct cctgctcccg 4200 ccgtgtccaa cgagttgctt gagaaagctg agacagttgt gatggaggtt ctcgctgcca 4260 agactggata cgaaacagac atgattgagg cggacatgga gcttgagact gagttgggaa 4320 ttgacagcat caagagggtt gaaattcttt ctgaagtcca agctatgctt aacgttgagg 4380 cgaaagatgt tgatgctctc tctaggacga gaacagtggg tgaagttgtc aacgctatga 4440 aggcagaaat cgctggttct tccgctccgg cgccggcagc tgccgcacct gcaccggcta 4500 aagcggctcc agctgcagct gcaccagctg tttctaatga gctgctcgaa aaggcagaaa 4560 ctgttgtgat ggaagtcctt gctgccaaaa ctggctacga aactgacatg atagaatcag 4620 acatggagct tgagacggag cttggaattg attccatcaa gcgtgtggaa atcctcagtg 4680 aagtccaagc catgctcaat gtggaagcta aggatgtgga tgctctctca aggaccagaa 4740 ctgtcggtga ggttgtcaat gccatgaagg ctgagatagc tggaggctcc gctcctgcgc 4800 cggccgctgc cgctcctggt cccgctgccg cggctccagc tccagctgca gccgctccgg 4860 ccgtctcaaa cgaactcctt gaaaaagctg agaccgtcgt gatggaggtc ttggcagcta 4920 aaaccggcta tgagactgac atgattgagt ctgacatgga attggagaca gaacttggga 4980 tagacagcat caaacgtgtt gaaatcctca gtgaggtcca agctatgctg aacgtggaag 5040 cgaaggatgt tgatgctctg tccagaacga ggaccgttgg tgaggttgtg gatgccatga 5100 aggctgaaat tgctggaggg tctgcgcccg cgccagctgc agccgctcct gcacccgcag 5160 ccgcggcacc tgctccagct gcacctgcac ctgccgtttc ttcagaactc ttggagaagg 5220 ctgagacggt tgtgatggag gttttggctg caaagactgg ctatgagacc gacatgattg 5280 aatctgatat ggaattggag acagagcttg ggatcgattc catcaaaaga gtggagatcc 5340 tgagtgaagt ccaagccatg ctcaatgttg aagctaagga tgttgatgca ctttcaagaa 5400 caaggacagt gggtgaggtt gtggatgcca tgaaggcgga gattgctgga gggagcgcgc 5460 ctgcgccagc tgccgcggca cctgcaccgg cagccgcagc tccagctcca gctgcaccag 5520 caccggccgc gcccgcgcca gctgtttcat ccgaactcct ggaaaaggcc gagacggttg 5580 tgatggaggt gctcgcagct aagactggtt acgagaccga catgattgaa agtgacatgg 5640 agcttgaaac cgagttggga atagatagca tcaaacgtgt tgaaatcttg tctgaggtcc 5700 aagctatgtt gaacgtggag gcaaaagatg tcgatgcgct ttcaagaacc agaacagtcg 5760 gtgaggtcgt ggacgccatg aaggctgaaa ttgccggaag ctctgctagt gcgccagctg 5820 cagccgcacc tgcacctgct gccgcggcac ctgctccagc tgcagccgct cctgccgtca 5880 gcaatgagtt gctggagaaa gccgaaacag ttgtgatgga agtccttgct gcaaaaactg 5940 gttacgaaac ggacatgatt gagtcagaca tggaattgga gaccgaactt ggaatagatt 6000 ccatcaagag agtggagatc ctctctgagg tgcaagccat gctcaacgtt gaggctaaag 6060 atgttgatgc actcagtagg acaagaactg tgggggaagt tgtcgatgcg atgaaggctg 6120 agattgctgg aggctctgct cctgcgccgg cagctgccgc acctgcaccc gcagccgcgg 6180 caccagctgt cagcaacgaa ttgcttgaga aggctgaaac agttgtcatg gaagtgctgg 6240 ctgcaaaaac cggctatgag actgacatga ttgagtctga catggaactt gagacggaat 6300 tgggcattga cagcatcaag cgtgttgaga tcctcagtga agtccaagca atgctcaatg 6360 tggaggctaa ggatgtggat gccctctcaa ggaccagaac agttggtgag gtcgttgatg 6420 cgatgaaggc ggagatcgct ggaagttccg cgcctgcgcc agctgcagcc gctcctgcac 6480 ccgcagccgc ggcaccggct ccagctgcag ccgcaccggc cgtctcctca gaactcctgg 6540 aaaaggctga gacagttgtg atggaagtcc ttgctgcgaa aactggctat gagaccgaca 6600 tgattgaatc tgacatggaa ctggagaccg agcttggaat tgactccatc aaacgtgttg 6660 aaatcctctc tgaggttcaa gcgatgttga atgtggaggc caaagatgtt gatgctcttt 6720 ccagaacaag gacggtggga gaggtggttg atgccatgaa ggctgagata gctgggggtt 6780 ctgctcctgc gccagctgca gccgctcctg ctcccgctgc cgcggcacca gctgtcagca 6840 acgaactcct tgaaaaagct gaaactgttg tgatggaggt tttggctgca aagactggat 6900 acgagactga catgatagag tcagacatgg agttggaaac agagcttggg attgatagca 6960 tcaagcgtgt ggaaatcctt tctgaggttc aagccatgct gaacgttgag gccaaagatg 7020 tcgatgcttt gtcaaggacc agaacggttg gagaagtggt cgatgccatg aaggctgaga 7080 tagctggtgg atcagcccct gctccggctg cagccgctcc tgcaagtgcc ggtgctgcac 7140 cagctgtgaa gattgattca gttcatggtg ctgactgtga cgatttgtca ctgatgcacg 7200 caaaggtggt tgacatcaga aggccagatg aactgattct ggaaagaccg gagaaccgtc 7260 cagttcttgt cgttgacgat ggctcagagt tgacacttgc tcttgtgagg gtgttgggag 7320 cctgtgcagt tgtcttgacc tttgagggat tgcagttggc tcagagagct ggtgcagctg 7380 ccattcgtca tgttctggct aaggaccttt cagcagaatc agctgagaag gccatcaaag 7440 aggctgagca aagatttgga gcgttgggag gcttcataag ccagcaagca gaaaggtttg 7500 aaccagctga gatactgggt ttcacactca tgtgtgcgaa gtttgccaaa gcctctctct 7560 gcactgctgt ggctggaggt cgtcctgcct tcatcggtgt ggctaggctt gacggcagac 7620 ttggattcac ctctcaaggc acttctgacg cactcaagag agcacagcgt ggagcgatct 7680 ttggactctg caagacaata gggttggagt ggtcagaatc tgatgtgttc agccgtggtg 7740 tggacattgc tcaagggatg cacccagaag atgctgcagt tgccatagtt agggagatgg 7800 cgtgtgctga catcagaatc agagaggttg ggattggagc aaaccagcaa agatgcacaa 7860 tcagagctgc caagttggag actgggaacc ctcagagaca gatagcgaaa gacgatgtgc 7920 ttctggtttc tggaggtgct cgtgggatca cacctctttg catccgtgag atcacgcgtc 7980 agattgctgg aggcaagtac attctgttgg gtcgtagcaa agtttctgcg tctgaaccag 8040 catggtgtgc tggcatcact gatgagaagg ctgtgcagaa agctgccacc caagagttga 8100 agagggcctt cagtgctggt gaaggaccca aacccactcc aagggctgtc acgaaacttg 8160 tgggatctgt tcttggtgct cgtgaagtga gaagctccat tgctgccatt gaagctctgg 8220 gtggcaaagc gatctacagc tcttgtgatg tgaactctgc tgcagatgtt gccaaagcag 8280 ttagggatgc agagtcccag cttggtgcca gagttagtgg aatcgttcac gcttctggtg 8340 tgctccgtga tcgtctcata gagaagaaac tccctgacga gtttgatgct gtctttggga 8400 ccaaagtcac tgggttggag aatcttctgg ctgcagttga tcgtgccaac ctcaaacaca 8460 tggttctctt cagctccctt gctggtttcc acggcaatgt tggtcagtct gactatgcga 8520 tggcaaacga agctctcaac aagatgggtt tggaacttgc gaaggatgtc agtgtcaagt 8580 ccatctgctt tggtccctgg gatggtggga tggtgacacc tcaactcaag aaacagtttc 8640 aagaaatggg agttcagatc attcctcgtg aaggaggtgc tgatacagtt gcgaggattg 8700 tgttgggatc aagtcctgct gagatccttg ttggaaactg gaggacacca tccaagaaag 8760 ttggaagtga cacgatcacc ctgcatagaa agatcagtgc caagtccaat cccttcttgg 8820 aggatcatgt catacaaggc agaagggtgt tgccaatgac ccttgctatt gggagccttg 8880 ctgagacctg ccttggactc ttccctggct actctctctg ggccatagat gacgcacagc 8940 tcttcaaagg tgtcactgtt gatggagacg tcaactgtga agtgaccctc acaccgtcca 9000 ctgctccgag tgggagggtg aatgtccaag cgacgctcaa gacgttctca agtggaaaac 9060 ttgttccagc ctacagagct gtcatagtgt tgtccaacca aggtgctcca cctgccaatg 9120 caaccatgca gcctccgagc cttgatgcag atcctgcttt gcaagggtca gtctatgatg 9180 gcaagactct ctttcatggt cctgcgttca gaggcattga tgacgttctc agctgcacca 9240 agagccagct tgttgccaag tgttctgctg ttcctggatc agatgcagcc agaggagagt 9300 ttgcaactga cactgatgca catgatccct ttgtcaatga tcttgccttt caagcaatgt 9360 tggtctgggt gaggagaacg ctgggacaag ctgcactccc gaactccatt cagaggatag 9420 tccaacacag accggtccca caagacaaac ccttctacat caccctcaga tccaatcaga 9480 gtggtggaca tagccagcac aaacatgcac ttcagttcca caacgaacaa ggtgatctct 9540 tcattgatgt tcaagcctct gtcattgcga cagactcact tgcgttctga gtagttagct 9600 taatcactta ggtcacccta tcaaaatcta tttagaaata cacaatattt tgttgcaggc 9660 ttgctggaga atcgatctgc tatcataaaa attacaaaaa aattttattt gcctcaatta 9720 ttttaggatt ggtattaagg acgcttaaat tatttgtcgg gtcactacgc atcattgtga 9780 ttgagaagat cagcgatacg aaatattcgt agtactatcg ataatttatt tgaaaattca 9840 taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata aagccacgca 9900 catttaggat attggccgag attactgaat attgagtaag atcacggaat ttctgacagg 9960 agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc catatgcagg 10020 agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca aggttgcggc 10080 cgcttaatta agatgagtga tactcaggac tcaggactca ctctgctgat cactagtgct 10140 agcctcgagg tcgacacaac tttgtataca aaagttgtct agacctaggc tcagcaactc 10200 atccagtcaa gttcgagtca gtgagttggg cggccgcgaa ttcggaaatg ggccaagtga 10260 aatggaaata gagcttcaat ccatttagtc ccactcaaaa tggtgctcga attatattta 10320 gttacgttcg aatcagacaa ccaagtattt ggttaataaa aaccactcgc aacaaaggaa 10380 aaacaccaag cgcgtgcgtc caacatccga cggaaggggg gtaatgtggt ccgaaaacct 10440 tacaaaaatc tgacgtcatc tacccccgaa aacgttgaat cgtcaacggg ggtagttttc 10500 gaattatctt ttttttaggg gcagttttat taatttgctc tagaaatttt atgattttaa 10560 ttaaaaaaag aaaaagaata tttgtatatt tattttttat actctttttt tgtccaacta 10620 tttctcttat tttggcaact ttaactagac tagtaactta tgtcaatgtg tatggatgca 10680 tgagagtgag tatacacatg tctaaatgca tgccttatga aagcaacgca ccacaaaacg 10740 aagacccctt tacaaataca tctcatccct tagtaccctc ttactactgt cccgacacaa 10800 actcaaaaca aggatccaca accaaaaatg gctgcaagga acgtgagtgc ggctcatgag 10860 atgcatgacg agaagaggat agctgttgtg ggcatggctg ttcagtatgc tggatgcaaa 10920 acgaaagacg agttctggga agttctcatg aatggaaaag ttgagagcaa agtgatcagc 10980 gacaaacgtc ttggttcaaa ttaccgtgct gaacactaca aagctgaaag gagcaagtat 11040 gctgacacct tctgcaatga gacatatgga actcttgatg agaacgaaat agacaatgag 11100 catgaactcc ttttgaacct tgccaaacaa gccttggctg aaacatctgt caaagattca 11160 acaagatgtg gcatagtttc tggatgcctc agcttcccga tggacaacct ccaaggtgaa 11220 ctcttgaatg tgtaccagaa ccatgttgag aagaaacttg gtgccagagt tttcaaagat 11280 gcctctcact ggtcagaaag agaacagtcc aacaaaccag aggctggtga cagaagaatc 11340 ttcatggacc ctgcttcctt tgttgctgag gaactcaatc ttggcgctct ccactactct 11400 gttgatgctg cctgtgccac tgcgctctat gtgctcagac tggcgcaaga tcacttggtc 11460 tctggtgcag ctgatgtgat gctttgtggt gcaacatgcc tgccggagcc gtttttcata 11520 ctctctgggt tctcaacctt ccaagccatg cctgttggaa cgggtcagaa tgtgtccatg 11580 ccgctgcaca aagattctca aggattgacc cctggtgaag gaggttccat catggtcctc 11640 aaacgtttgg atgacgcaat cagagatgga gatcacatct atggaacact tttgggagcc 11700 aatgtcagca acagtggcac tggtctgcca cttaaacccc ttttgccgtc agagaaaaag 11760 tgcctcatgg acacctacac acgtatcaat gtgcatcccc acaagattca gtatgttgaa 11820 tgccatgcaa ctgggactcc acaaggtgat cgtgtggaaa ttgatgctgt gaaggcttgc 11880 tttgaaggca aagtcccacg ttttggaacc acaaaaggga actttggaca cacattggtg 11940 gcagctgggt ttgctggcat gtgcaaagtg cttctctcca tgaagcacgg gatcattcca 12000 cccactccgg gaatagatga cgaaacgaag atggaccctc tggttgtctc tggtgaggcc 12060 attccctggc cagaaacgaa tggagaaccg aagcgtgctg gtctctcagc gtttggattt 12120 ggtgggacca atgctcatgc tgtctttgag gaacatgacc caagcaatgc tgcatgcact 12180 ggccatgact ccatttctgc tctctctgca cgttgtggtg gtgagtcaaa catgagaata 12240 gcaatcactg ggatggatgc cacttttggt gctttgaaag gattggatgc ttttgaaagg 12300 gccatctaca ctggtgctca tggtgctata ccactcccag aaaagagatg gagattcctg 12360 gggaaagaca aggacttcct tgatctttgt ggagtcaaag caacaccgca tggctgttac 12420 attgaagatg ttgaagttga tttccagagg ctgaggactc caatgactcc tgaagatatg 12480 ctcttgcctc aacagcttct ggctgtcacc acaattgata gggccatatt ggactctggc 12540 atgaagaaag gtggaaatgt ggctgtcttt gtggggttgg ggaccgatct tgaactctac 12600 cgtcaccgtg caagggttgc tctcaaggag agagtgaggc ctgaagccag caagaaactc 12660 aatgacatga tgcagtacat caatgattgt gggacaagca cttcttacac cagctacata 12720 ggcaacttgg ttgcaacaag ggtcagttca caatggggct tcacgggacc ttccttcaca 12780 atcacagaag gcaacaattc tgtgtacaga tgtgctgaac ttggcaaata cctcttggaa 12840 acgggagagg ttgatggggt tgtggttgcg ggtgttgatc tctgtgggag tgctgagaac 12900 ctttacgtca agtcaaggcg tttcaaggtg tctacatctg acaccccgag agccagcttt 12960 gatgcagctg ctgatggtta ctttgttgga gagggctgtg gtgcatttgt gctcaagcgt 13020 gagacaagct gcaccaagga tgacagaatc tatgcttgca tggatgccat agttcctgga 13080 aatgttccat ctgcatgttt gagagaggca ttggatcaag ccagagtcaa gcctggagac 13140 atcgagatgt tggagctttc tgctgattca gcgaggcatt tgaaggaccc gtctgttctc 13200 ccaaaggaac tcactgctga ggaagagatt ggcggacttc agacgattct tcgtgacgat 13260 gacaagctcc caaggaatgt tgcaactgga agtgtcaagg ccactgttgg tgacactggt 13320 tatgcttctg gtgctgccag tttgattaag gctgcgcttt gcatctacaa ccgttacctg 13380 cccagcaatg gagatgactg ggatgaacct gcacctgaag caccctggga cagcacactc 13440 tttgcgtgcc agacctcaag agcttggttg aagaaccctg gtgagagacg ttatgcggct 13500 gtttccggag tcagtgaaac cagaagttgc tacagtgtgc tgctttctga agcagaggga 13560 cactacgaga gagagaacag aatcagtttg gatgaggaag cacccaagct cattgtgttg 13620 agagcagact cccatgaaga gattcttgga agattggaca agataaggga aaggttcttg 13680 cagcccacgg gtgctgcacc cagagagtct gaattgaagg cgcaagcccg taggatcttt 13740 cttgaactcc ttggtgagac ccttgcccaa gatgcagctt catctggaag tcagaagccc 13800 cttgctctca gcttggtctc cactccatcc aagctccaga gagaggtgga acttgcggca 13860 aaaggcattc cgagatgtct caagatgaga cgtgattgga gttcccctgc tggaagcaga 13920 tatgcgcctg agcctttggc cagtgacaga gttgccttca tgtatggaga aggaaggagc 13980 ccttactatg gcatcacaca agacattcat aggatttggc ctgaacttca tgaggtcatc 14040 aatgagaaaa caaaccgtct ctgggctgaa ggtgacagat gggtcatgcc aagggccagc 14100 ttcaagagtg agcttgagtc acaacagcaa gagtttgaca gaaacatgat tgaaatgttc 14160 cgtcttggaa tcctcacctc aattgccttc acaaacctgg cgagagatgt tctcaacatc 14220 acgcccaaag cagcctttgg actcagtttg ggagagattt ccatgatctt tgctttctcc 14280 aagaaaaacg gtttgataag cgatcagttg acaaaggatt tgagagagtc agatgtctgg 14340 aacaaagctc tggctgttga gttcaatgcg ctcagagaag cctggggcat tccacagtct 14400 gtcccaaaag atgagttctg gcaaggttac atagtgaggg gaacgaaaca agacatagaa 14460 gcagccatag caccagactc aaagtatgtg aggttgacca tcatcaatga tgccaacact 14520 gctctcatca gtggaaagcc tgatgcatgc aaagctgcga ttgctcgtct tggtggcaac 14580 atccctgcgc ttccagtcac tcaaggcatg tgtgggcatt gcccagaggt gggaccttac 14640 accaaggaca tcgcaaagat ccacgccaac cttgagttcc ctgtggttga tggtcttgat 14700 ctctggacta ccataaacca gaagaggctt gtgccaagag ccacgggtgc caaagatgaa 14760 tgggctcctt catcctttgg ggagtatgct ggacagctct atgagaaaca agccaacttc 14820 cctcagatag ttgaaaccat ctacaagcag aactatgatg tgtttgttga agttgggcca 14880 aacaatcaca gaagcacagc agtgagaacc acgcttggac cacagagaaa ccaccttgct 14940 ggtgccatag acaagcagaa cgaggatgct tggacgacaa tagtcaagct cgtggcttca 15000 ttgaaggctc accttgttcc tggagtgacc atcagtccgc tctaccactc caagctcgtt 15060 gcagaggctg aggcttgtta tgctgcgctc tgtaaaggtg agaaacccaa gaaaaacaag 15120 tttgtgagaa agattcagtt gaatggcaga ttcaacagca aggcagaccc catctcctca 15180 gcagaccttg catccttccc acctgcggac cctgcgattg aagctgccat ctcttcaagg 15240 atcatgaagc ctgtggctcc gaaattctat gcgcgtctca acattgatga gcaagatgag 15300 accagagatc cgatcttgaa caaggacaat gcgccttcca gctccagctc ttccagcagt 15360 tcctcttcca gcagttcatc tccgtctcct gctccttcag ctccagttca gaagaaagct 15420 gccccagctg cggagacaaa agctgttgcc agtgctgatg cacttcgtag tgccctgctt 15480 gatcttgact ccatgcttgc gctcagctct gcaagtgctt ctggcaacct tgttgagact 15540 gcgccatctg atgccagtgt cattgtccct ccgtgcaaca ttgctgatct tggcagcaga 15600 gctttcatga aaacttatgg tgtttctgcg cctctgtaca ccggtgcaat ggccaaggga 15660 attgcctctg cggacttggt gatcgcagct ggaaggcaag gaatccttgc gtcatttggt 15720 gctggaggcc ttccaatgca agttgtgcgt gagtccattg aaaagattca agctgccctg 15780 cccaatggac catatgctgt caacctcatc cattctcctt ttgacagcaa ccttgagaag 15840 ggaaatgttg atctcttctt ggaaaagggt gtcacttttg ttgaggcttc agccttcatg 15900 acgttgacac cgcaagttgt cagatacaga gcggctgggt tgacgaggaa tgcagatggg 15960 tcagtcaaca taaggaacag aatcattggg aaagtctcca gaactgagct ggctgaaatg 16020 ttcatgcgtc ctgcacctga gcatcttttg cagaagctca ttgcttctgg ggagatcaat 16080 caagaacaag ctgagttggc cagacgtgtt cctgtggctg atgacattgc ggttgaagct 16140 gattctggtg gacacactga caaccgtcca atccatgtca ttctgcctct gattatcaac 16200 ctgagggata ggcttcacag agagtgtgga tacccagcaa acctgagggt tcgtgttggt 16260 gctgggggtg gcattgggtg tccccaagct gcgctggcaa ctttcaacat gggtgcctct 16320 ttcattgtca ctggaacagt gaaccaagtt gccaaacaat ctgggacgtg tgacaatgtg 16380 aggaaacagc ttgcgaaagc aacttactca gatgtttgca tggcaccggc agctgacatg 16440 tttgaggaag gtgtgaaact tcaagttttg aagaaaggaa caatgttccc ttcaagagcc 16500 aacaaactct atgagttgtt ctgcaagtat gattcttttg agtccatgcc tccagcagag 16560 cttgcgaggg ttgagaaaag aatcttcagt cgtgcattgg aagaggtctg ggatgagacc 16620 aagaacttct acatcaaccg tctccacaac ccagagaaga tacagagggc tgagagggac 16680 cccaaactga agatgtcact gtgtttcaga tggtatctgt ccctggcttc aaggtgggcc 16740 aacactggag cttctgacag agtcatggac taccaagttt ggtgtggtcc tgccattggt 16800 tcattcaatg atttcatcaa aggaacttac cttgatccgg cagtggcaaa tgagtacccg 16860 tgtgttgtgc agatcaacaa acagattctt agaggagcgt gcttcttgag gcgtcttgaa 16920 atactgagaa atgcaaggct ttcagatggt gcagctgcac ttgtggcgag cattgatgac 16980 acatatgtcc ctgctgagaa gctgtgagta gttagcttaa tcacttaggt caccctatca 17040 aaatctattt agaaatacac aatattttgt tgcaggcttg ctggagaatc gatctgctat 17100 cataaaaatt acaaaaaaat tttatttgcc tcaattattt taggattggt attaaggacg 17160 cttaaattat ttgtcgggtc actacgcatc attgtgattg agaagatcag cgatacgaaa 17220 tattcgtagt actatcgata atttatttga aaattcataa gaaaagcaaa cgttacatga 17280 attgatgaaa caatacaaag acagataaag ccacgcacat ttaggatatt ggccgagatt 17340 actgaatatt gagtaagatc acggaatttc tgacaggagc atgtcttcaa ttcagcccaa 17400 atggcagttg aaatactcaa accgccccat atgcaggagc ggatcattca ttgtttgttt 17460 ggttgccttt gccaacatgg gagtccaagg ttgcggccgc ttaattaatc tgacctgacc 17520 actagacctg agtgagtaca gatgtgctac tagtgctagc ctcgaggtcg accaactttt 17580 ctatacaaag ttgtctagac ctaggactag gcactgagtg actcactgac ctgactgagt 17640 gactggcggc cgcgcggccg cgaattcgga aatgggccaa gtgaaatgga aatagagctt 17700 caatccattt agtcccactc aaaatggtgc tcgaattata tttagttacg ttcgaatcag 17760 acaaccaagt atttggttaa taaaaaccac tcgcaacaaa ggaaaaacac caagcgcgtg 17820 cgtccaacat ccgacggaag gggggtaatg tggtccgaaa accttacaaa aatctgacgt 17880 catctacccc cgaaaacgtt gaatcgtcaa cgggggtagt tttcgaatta tctttttttt 17940 aggggcagtt ttattaattt gctctagaaa ttttatgatt ttaattaaaa aaagaaaaag 18000 aatatttgta tatttatttt ttatactctt tttttgtcca actatttctc ttattttggc 18060 aactttaact agactagtaa cttatgtcaa tgtgtatgga tgcatgagag tgagtataca 18120 catgtctaaa tgcatgcctt atgaaagcaa cgcaccacaa aacgaagacc cctttacaaa 18180 tacatctcat cccttagtac cctcttacta ctgtcccgac acaaactcaa aacaaggatc 18240 cacaaccaaa aatggcgctt cgtgtcaaga ccaacaagaa gccatgttgg gagatgacca 18300 aggaggaact gacctctggc aaaactgagg tgttcaacta tgaggaactc ctggagtttg 18360 cagagggtga catagctaag gtttttggtc cggagtttgc tgtcatagac aagtacccca 18420 gaagggtgag gctgcctgct cgtgaatacc tgcttgtgac aagggtcaca ctcatggatg 18480 ccgaggtcaa caactacaga gttggtgcca gaatggtcac tgagtatgat cttccagtga 18540 atggcgaatt gtctgaggga ggtgattgtc cctgggcagt tctggttgag agtggacagt 18600 gcgatttgat gctgatatca tacatgggaa ttgatttcca gaaccaaggt gacagagttt 18660 accgtctgct caacaccacg ttgacattct atggggttgc ccatgaggga gagacccttg 18720 agtatgacat cagagtgacc ggttttgcca aacgtttgga tggtggcatc tccatgttct 18780 tctttgagta tgactgctat gtcaatggga ggcttctcat tgagatgaga gatggctgtg 18840 ctggcttctt cacaaatgag gagcttgatg cgggaaaggg tgtggtgttc acaagagggg 18900 atttggctgc cagagctaag attccaaagc aagatgtttc tccctatgct gtcgctccct 18960 gccttcacaa aacaaaactc aacgaaaagg agatgcagac cttggttgac aaagattggg 19020 cttccgtttt tggctcaaag aatggcatgc cggagatcaa ctacaaactc tgcgcacgta 19080 agatgctcat gattgataga gtcaccagca ttgaccataa gggaggtgtg tacggtttgg 19140 gtcagttggt tggtgagaaa atcttggaga gggaccattg gtacttccca tgccattttg 19200 ttaaggatca agtcatggct ggatctcttg tcagcgacgg ttgttcacaa atgctcaaga 19260 tgtacatgat ctggctcggt ttgcacctta ccaccggacc atttgacttc agacccgtca 19320 acggacaccc gaacaaagtt aggtgtaggg gtcagatatc tcctcataag ggaaagttgg 19380 tctacgtcat ggagatcaaa gagatgggat tcgacgagga taacgatccg tacgccattg 19440 ctgacgtcaa catcattgac gttgatttcg aaaagggtca agatttcagc cttgatagaa 19500 tcagcgacta cggaaagggg gatcttaaca aaaagatcgt cgtggacttc aagggcattg 19560 ctctcaagat gcagaagaga tccacgaaca agaacccctc caaagttcag cccgtttttg 19620 ctaacggtgc agccactgtg ggaccggaag cgtccaaggc cagctctggt gccagtgcta 19680 gtgcttctgc tgcccctgcg aagcctgctt tctcagcaga tgttcttgcg ccaaagccag 19740 ttgcacttcc tgaacatatc ctcaagggtg acgctctcgc tccgaaagaa atgtcctggc 19800 atcccatgtc aaaacttcct ggcaacccga cgccgttctt ctcccctagt tcttaccctc 19860 cgagggcaat ttgcttcatc cctttccctg gcaacccact tgacaacaat tgcaaagctg 19920 gagaaatgcc tctgaactgg tacaacatgt cagagttcat gtgtggaaaa gttagcaact 19980 gcttgggacc agagttcgca agatttgata agtcaaatac gagcagaagc cctgcttttg 20040 atttggctct tgtgactaga gttgttgaag tcacaaacat ggaacatggc aagttcctga 20100 atgttgattg caatcccagc aaaggcacaa tggtggggga gtttgactgt cctcaagatg 20160 cgtggttctt tgatggttct tgcaatgatg ggcacatgcc ttattccatc atcatggaaa 20220 tcggacttca gacatccggt gttctcacca gtgtgttgaa agcaccactg acgatggaca 20280 aagatgacat tctctttagg aaccttgatg caagtgctga aatggtgagg ccagatgtgg 20340 atgtgagggg gaaaaccata aggaatgtga ccaagtgcac tggatatgca atgttgggaa 20400 agatggggat tcacagattc acgtttgagt tgtctgttga tggcgtggtt ttctacaaag 20460 gaagcacttc ctttggatgg ttcactccag aggtgtttgc tcagcaagct ggacttgaca 20520 atgggaaaaa gacggagcct tggtgcaaaa ctaacaacac gagtgtgaga agagttgaaa 20580 tcgcatcagc caaaggcaaa gaacagctga ctgagaaact tccagatgca actaatgctc 20640 aagttctcag aaggtcagaa cagtgtgagt atttggatta cctcaacatt gctcctgatt 20700 ctgggctgca tgggaaagga tatgcgcatg gacacaaaga tgttaaccca caagactggt 20760 tcttctcttg tcacttttgg tttgatcctg tgatgcctgg atctttggga attgagtcaa 20820 tgttccagct cattgaggca tttgcggtgg accagaacat accgggagaa tacaatgtga 20880 gcaatccgac ctttgcccat gcccctggaa aaacggcgtg gaagtaccgt ggtcaattga 20940 caccaaagaa ccgtgcgatg gactgtgagg tgcacatagt ttcaatcaca gcctctcctg 21000 aaaatggagg ctacgttgac atagtggcag atggagcact ttgggttgat ggtctccgtg 21060 tgtatgaagc caaagaactc agagttcgtg ttgtcagtgc aaaacctcaa gccattccgg 21120 atgttcaaca acagccaccc agcgcaaaag cggaccctgg gaaaactgga gttgcactca 21180 gtccgactca gttgagagat gtcctgcttg aagtggacaa tccattgtat cttggtgttg 21240 aaaactccaa tttggtgcag tttgaatcca aacctgcaac ttcttcaagg attgtcagta 21300 tcaaaccatg ctccataagt gatcttggtg acaagtcctt catggaaacg tacaatgtgt 21360 cagcacctct gtacactgga gcaatggcca agggaattgc gtctgcggac cttgtcattg 21420 ctgctgggaa gaggaagata ttgggttctt ttggagcggg tgggcttcca atgcaccatg 21480 tgagggcagc tttggagaag atacaagcgg ctctgcctca aggaccatat gctgtgaacc 21540 tcattcattc accttttgac agcaacttgg aaaaggggaa tgttgatctc ttcctcgaaa 21600 agggcgttac tgtggtggag gcttctgcat tcatgaccct cactccccaa gttgttcgtt 21660 accgtgcagc cggactctca aggaacgctg atgggagtgt caacatcaga aaccgtatca 21720 ttggaaaagt gtccagaaca gaactcgccg aaatgttcat cagaccggct ccggaacacc 21780 tccttgagaa actcatagct tctggtgaga taacacaaga acaagccgaa cttgcccgtc 21840 gtgttcctgt cgcagacgac atcgctgttg aagctgattc tggtggtcac actgacaacc 21900 gtcccatcca cgtcatcttg cctttgatca tcaaccttag gaacagactg cacagagagt 21960 gtggttatcc tgcgcacctt agagtcagag ttggagctgg aggcggtgtt ggctgcccac 22020 aagctgctgc ggctgccctc acaatgggag ctgccttcat agtgactggg actgtcaatc 22080 aagttgctaa acagtccggg acatgtgaca acgtgagaaa acaactcagt caagccacct 22140 acagtgacat ttgcatggct ccagcagctg atatgtttga ggaaggtgtg aaactccaag 22200 ttctcaagaa gggaacaatg ttcccatcaa gagctaacaa attgtacgaa ctcttttgca 22260 agtacgactc tttcgattca atgcctcctg ctgaacttga aaggatcgag aagaggatct 22320 tcaagagggc acttcaagag gtctgggaag agaccaagga cttttacatc aatggtttga 22380 agaatccaga gaagatacag agagcggaac atgaccccaa actcaagatg tctttgtgtt 22440 tcagatggta tcttggtctt gcatcacgtt gggccaacat gggtgctcct gatcgtgtca 22500 tggactatca agtctggtgt ggtccagcca ttggagcctt caatgacttc atcaaaggaa 22560 catatcttga tcctgctgtc tctaatgagt acccatgtgt tgtccagatc aaccttcaga 22620 ttttgcgtgg tgcatgttac ctgaggcgtc ttaatgctct gagaaatgat ccgaggattg 22680 atcttgagac tgaagatgct gcctttgtct atgagccaac gaatgcgctt tgagtagtta 22740 gcttaatcac ttaggtcacc ctatcaaaat ctatttagaa atacacaata ttttgttgca 22800 ggcttgctgg agaatcgatc tgctatcata aaaattacaa aaaaatttta tttgcctcaa 22860 ttattttagg attggtatta aggacgctta aattatttgt cgggtcacta cgcatcattg 22920 tgattgagaa gatcagcgat acgaaatatt cgtagtacta tcgataattt atttgaaaat 22980 tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag ataaagccac 23040 gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg aatttctgac 23100 aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg ccccatatgc 23160 aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt ccaaggttgc 23220 ggccgcttaa ttaatcacga gtggatgact gtcacgactg actcatgact gactactagt 23280 gctagcctcg aggtcgacac aactttgtat aataaagttg tctagaccta ggagctgtgc 23340 tgggtaactc acatgagtca ctggtcacat cagcggccgc gaattcggaa atgggccaag 23400 tgaaatggaa atagagcttc aatccattta gtcccactca aaatggtgct cgaattatat 23460 ttagttacgt tcgaatcaga caaccaagta tttggttaat aaaaaccact cgcaacaaag 23520 gaaaaacacc aagcgcgtgc gtccaacatc cgacggaagg ggggtaatgt ggtccgaaaa 23580 ccttacaaaa atctgacgtc atctaccccc gaaaacgttg aatcgtcaac gggggtagtt 23640 ttcgaattat ctttttttta ggggcagttt tattaatttg ctctagaaat tttatgattt 23700 taattaaaaa aagaaaaaga atatttgtat atttattttt tatactcttt ttttgtccaa 23760 ctatttctct tattttggca actttaacta gactagtaac ttatgtcaat gtgtatggat 23820 gcatgagagt gagtatacac atgtctaaat gcatgcctta tgaaagcaac gcaccacaaa 23880 acgaagaccc ctttacaaat acatctcatc ccttagtacc ctcttactac tgtcccgaca 23940 caaactcaaa acaaggatcc acaaccaaaa atgcttcagc acacttggct tccgaagcct 24000 cccaatctga ccctcttgtc agatgaggtt catctctgga ggattcctct tgaccagcct 24060 gagtcacaac ttcaagacct tgctgccacc ctgagcagtg atgaattggc gagggcaaac 24120 agattctact ttccagaaca cagaaggcgt ttcactgctg ggagaggcat cctcagatcc 24180 atcttgggtg gatacttggg agtggaaccg ggtcaagtca agtttgatta tgagtcccgt 24240 gggaaaccga tccttggtga cagatttgct gagagtggac tcctgttcaa cttgtctcac 24300 agccagaacc ttgccttgtg tgctgtcaac tacacgcgtc aaataggcat tgatcttgaa 24360 tatctgcgtc caacatctga cttggagtct cttgcaaaga ggttctttct cccaagagaa 24420 tatgaactct tgaggtcact ccctgatgag cagaaacaga agattttctt tcgttactgg 24480 acttgcaaag aggcttatct caaagcaacg ggagatggaa tagccaaact tgaagagatc 24540 gagatagcac tcaccccaac agaacctgcc aagctccaaa cagctcctgc gtggtctctg 24600 ttggagcttg tgccagatga caattgtgtt gcagctgtgg ctgttgcggg ttttggttgg 24660 cagcccaagt tctggcatta ctgagtagtt agcttaatca cttaggtcac cctatcaaaa 24720 tctatttaga aatacacaat attttgttgc aggcttgctg gagaatcgat ctgctatcat 24780 aaaaattaca aaaaaatttt atttgcctca attattttag gattggtatt aaggacgctt 24840 aaattatttg tcgggtcact acgcatcatt gtgattgaga agatcagcga tacgaaatat 24900 tcgtagtact atcgataatt tatttgaaaa ttcataagaa aagcaaacgt tacatgaatt 24960 gatgaaacaa tacaaagaca gataaagcca cgcacattta ggatattggc cgagattact 25020 gaatattgag taagatcacg gaatttctga caggagcatg tcttcaattc agcccaaatg 25080 gcagttgaaa tactcaaacc gccccatatg caggagcgga tcattcattg tttgtttggt 25140 tgcctttgcc aacatgggag tccaaggttg cggccgctta attaactact gtcactgagg 25200 ccgtagacga gtacggactg atctaactag tgctagcctc gaggtcgaca cccagctttc 25260 ttgtacaaag tggttgcggc cgcttaatta aatttaaatt caattaatgc aatcttgatt 25320 ttcaacaacg aaggtaatgg cgtaaaagaa aaaatgtatg ttattgtatt gatctttcat 25380 gatgttgaag cgtgccataa tatgatgatg tataattaaa atattaactg tcgcatttta 25440 ttgaaatggc actgttattt caaccatatc tttgattctg ttacatgaca cgactgcaag 25500 aagtaaataa tagacgccgt tgttaaagaa ttgctatcat atgtgcctaa ctagagggaa 25560 tttgagcgtc agacctaatc aaatattaca aaatatctca ctctgtcgcc agcaatggtg 25620 taatcagcgc agacaaatgg cgtaaagatc gcggaaaaac ctccccgagt ggcatgatag 25680 ctgcctctgt attgctgatt tagtcagcct tatttgactt aagggtgccc tcgttagtga 25740 caaattgctt tcaaggagac agccatgccc cacactttgt tgaaaaacaa attgcctttg 25800 gggagacggt aaagccagtt gctcttcaat aaggaatgtc gaggaggcaa tgtaaccgcc 25860 tctggtagta cacttctcta atccaaaaat caatttgtat tcaagatacc gcaaaaaact 25920 tatggtttaa accctgcagg actagtccag aaggtaatta tccaagatgt agcatcaaga 25980 atccaatgtt tacgggaaaa actatggaag tattatgtaa gctcagcaag aagcagatca 26040 atatgcggca catatgcaac ctatgttcaa aaatgaagaa tgtacagata caagatccta 26100 tactgccaga atacgaagaa gaatacgtag aaattgaaaa agaagaacca ggcgaagaaa 26160 agaatcttga agacgtaagc actgacgaca acaatgaaaa gaagaagata aggtcggtga 26220 ttgtgaaaga gacatagagg acacatgtaa ggtggaaaat gtaagggcgg aaagtaacct 26280 tatcacaaag gaatcttatc ccccactact tatcctttta tatttttccg tgtcattttt 26340 gcccttgagt tttcctatat aaggaaccaa gttcggcatt tgtgaaaaca agaaaaaatt 26400 tggtgtaagc tattttcttt gaagtactga ggatacaact tcagagaaat ttgtaagttt 26460 gtaggtacca gatctggatc ccaaaccatg tctccggaga ggagaccagt tgagattagg 26520 ccagctacag cagctgatat ggccgcggtt tgtgatatcg ttaaccatta cattgagacg 26580 tctacagtga actttaggac agagccacaa acaccacaag agtggattga tgatctagag 26640 aggttgcaag atagataccc ttggttggtt gctgaggttg agggtgttgt ggctggtatt 26700 gcttacgctg ggccctggaa ggctaggaac gcttacgatt ggacagttga gagtactgtt 26760 tacgtgtcac ataggcatca aaggttgggc ctaggatcta cattgtacac acatttgctt 26820 aagtctatgg aggcgcaagg ttttaagtct gtggttgctg ttataggcct tccaaacgat 26880 ccatctgtta ggttgcatga ggctttggga tacacagccc ggggtacatt gcgcgcagct 26940 ggatacaagc atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca 27000 gctcctccaa ggccagttag gccagttacc caaatctgag tagttagctt aatcacctag 27060 agctcgatcg gcggcaatag cttcttagcg ccatcccggg ttgatcctat ctgtgttgaa 27120 atagttgcgg tgggcaaggc tctctttcag aaagacaggc ggccaaagga acccaaggtg 27180 aggtgggcta tggctctcag ttccttgtgg aagcgcttgg tctaaggtgc agaggtgtta 27240 gcgggatgaa gcaaaagtgt ccgattgtaa caagatatgt tgatcctacg taaggatatt 27300 aaagtatgta ttcatcacta atataatcag tgtattccaa tatgtactac gatttccaat 27360 gtctttattg tcgccgtatg taatcggcgt cacaaaataa tccccggtga ctttctttta 27420 atccaggatg aaataatatg ttattataat ttttgcgatt tggtccgtta taggaattga 27480 agtgtgcttg aggtcggtcg ccaccactcc catttcataa ttttacatgt atttgaaaaa 27540 taaaaattta tggtattcaa tttaaacacg tatacttgta aagaatgata tcttgaaaga 27600 aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc caagcaaaaa 27660 cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat tatatcagct 27720 ggtacattgc cgtagatgaa agactgagtg cgatattatg gtgtaataca taggaattcg 27780 tttaaacgat ctgcgtctaa ttttcggtcc aacttgcaca ggaaagacgt cgaccgcggt 27840 agctcttgcc cagcagactg ggcttccagt cctttcgctc gatcgggtcc aatgttgtcc 27900 tcagctgtga accggaagcg gacgaccaac agtggaagaa ctgaaaggaa cgagccgtct 27960 ataccttgat gatcggcctc tggtgaaggg tatcatcgca gccaagcaag ctcatgaaag 28020 gctgatgggg gaggtgtata attatgaggc ccacggcggg cttattcttt agggaggatc 28080 tatctcgttg ctcaagtgca tggcgcaaag cagttattgg agtgcggatt ttcgttggca 28140 tattattcgc cacgagttag cagacgaaga gaccttcatg aacgtggcca aggccagagt 28200 taagcagatg ttacgccctg ctgcaggcct ttctattatc caatagttgg ttgatctttg 28260 gaaagagcct cggctgaggc ccatactgaa agagatcgat ggatatcgat atgccatgtt 28320 gtttgctagc cagaaccaga tcacatccga tatgctattg cagcttgacg cagatatgga 28380 ggataagttg attcatggga tcgctcagga gtagctcatc catgcacgcc gacaagaaca 28440 gaaattccgt cgagttaacg cagccgctta cgacggattc gaaggtcatc cattcggaat 28500 gtattagttt gcaccagctc cgcgtcacac ctgtcttcat ttgaataaga tgttagcaat 28560 tgtttttagc tttgtcttgt tgtggcaggg cggcaagtgc ttcagacatc attctgtttt 28620 caaattttat gctggagaac agcttcttaa ttcctttgga aataatagac tgcgtcttaa 28680 aattcagatg tctggatata gatatgattg taaaataacc tatttaagtg tcatttagaa 28740 cataagtttt atgaatgttc ttccattttc gtcatcgaac gaataagagt aaatacacct 28800 tttttaacat tacaaataag ttcttatacg ttgtttatac accgggaatc atttccatta 28860 ttttcgcgca aaagtcacgg atattcgtga aagcgacata aactgcgaaa tttgcgggga 28920 gtgtcttgag tttgcctcga ggctagcgca tgcacataga cacacacatc atctcattga 28980 tgcttggtaa taattgtcat tagattgttt ttatgcatag atgcactcga aatcagccaa 29040 ttttagacaa gtatcaaacg gatgtgactt cagtacatta aaaacgtccg caatgtgtta 29100 ttaagttgtc taagcgtcaa tttgatttac aattgaatat atcctgcccc agccagccaa 29160 cagctcgatt tacaattgaa tatatcctgc cggccggccc acgcgtgtcg aggaattctg 29220 atctggcccc catttggacg tgaatgtaga cacgtcgaaa taaagatttc cgaattagaa 29280 taatttgttt attgctttcg cctataaata cgacggatcg taatttgtcg ttttatcaaa 29340 atgtactttc attttataat aacgctgcgg acatctacat ttttgaattg aaaaaaaatt 29400 ggtaattact ctttcttttt ctccatattg accatcatac tcattgctga tccatgtaga 29460 tttcccggac atgaagccat ttacaattga atatatcctg ccgccgctgc cgctttgcac 29520 ccggtggagc ttgcatgttg gtttctacgc agaactgagc cggttaggca gataatttcc 29580 attgagaact gagccatgtg caccttcccc ccaacacggt gagcgacggg gcaacggagt 29640 gatccacatg ggacttttaa acatcatccg tcggatggcg ttgcgagaga agcagtcgat 29700 ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg atcgcaaagt atttgaacgc 29760 aggtacaatc gagccgacgt tcacgcggaa cgaccaagca agcttggctg ccatttttgg 29820 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc 29880 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca 29940 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga 30000 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg 30060 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa 30120 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca 30180 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt 30240 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct 30300 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc 30360 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg 30420 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 30480 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat 30540 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 30600 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 30660 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 30720 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 30780 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc 30840 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg 30900 cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac aaaatcacgg 30960 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc 31020 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg 31080 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca 31140 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg 31200 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg 31260 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg 31320 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc 31380 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga 31440 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg 31500 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa 31560 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc 31620 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt 31680 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg 31740 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag 31800 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc 31860 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc 31920 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc 31980 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac 32040 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc 32100 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac 32160 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac 32220 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc 32280 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc 32340 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg 32400 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc 32460 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc 32520 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg 32580 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct 32640 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc 32700 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattg 32760 tgattaaggc tcagattcga cggcttggag cggccgacgt gcaggatttc cgcgagatcc 32820 gattgtcggc cctgaagaaa gctccagaga tgttcgggtc cgtttacgag cacgaggaga 32880 aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc cgtggcattc ggcgcctaca 32940 tcgacggcga gatcattggg ctgtcggtct tcaaacagga ggacggcccc aaggacgctc 33000 acaaggcgca tctgtccggc gttttcgtgg agcccgaaca gcgaggccga ggggtcgccg 33060 gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt gatgatcgtc cgacagattc 33120 caacgggaat ctggtggatg cgcatcttca tcctcggcgc acttaatatt tcgctattct 33180 ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg 33240 ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct gctaggtagc ccgatacgat 33300 tgatggcggt cctgggggct atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac 33360 caaacgcagc gctagatcct gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg 33420 cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt gcctctgctc acctttaccg 33480 cctggcaact ggcggccgga ggacttctgc tcgttccagt agctttagtg tttgatccgc 33540 caatcccgat gcctacagga accaatgttc tcggcctggc gtggctcggc ctgatcggag 33600 cgggtttaac ctacttcctt tggttccggg ggatctcgcg actcgaacct acagttgttt 33660 ccttactggg ctttctcagc ccccgagcgc ttagtgggaa tttgtacccc ttatcgaacc 33720 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt 33780 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 33840 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 33900 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 33960 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 34020 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 34080 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 34140 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 34200 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 34260 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 34320 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 34380 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 34440 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca 34500 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg 34560 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt 34620 cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa 34680 ggtggttcta agcctcgtct tgcgatggca tttcgatcca ttcccattcc gcgctcaaga 34740 tggcttcccc tcggcagttc atcagggcta aatcaatcta gccgacttgt ccggtgaaat 34800 gggctgcact ccaacagaaa caatcaaaca aacatacaca gcgacttatt cacacgagct 34860 ca 34862 <210> 47 <211> 34404 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9167 <400> 47 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcgaa ttcggaaatg ggccaagtga aatggaaata 300 gagcttcaat ccatttagtc ccactcaaaa tggtgctcga attatattta gttacgttcg 360 aatcagacaa ccaagtattt ggttaataaa aaccactcgc aacaaaggaa aaacaccaag 420 cgcgtgcgtc caacatccga cggaaggggg gtaatgtggt ccgaaaacct tacaaaaatc 480 tgacgtcatc tacccccgaa aacgttgaat cgtcaacggg ggtagttttc gaattatctt 540 ttttttaggg gcagttttat taatttgctc tagaaatttt atgattttaa ttaaaaaaag 600 aaaaagaata tttgtatatt tattttttat actctttttt tgtccaacta tttctcttat 660 tttggcaact ttaactagac tagtaactta tgtcaatgtg tatggatgca tgagagtgag 720 tatacacatg tctaaatgca tgccttatga aagcaacgca ccacaaaacg aagacccctt 780 tacaaataca tctcatccct tagtaccctc ttactactgt cccgacacaa actcaaaaca 840 aggatccaca accaaaaatg gcagcgaggc tgcaagagca gaaaggaggt gagatggaca 900 cgaggattgc gatcataggc atgagcgcca tacttccttg tggaaccacg gtgagagagt 960 catgggagac cataagggct ggcattgatt gcctgtctga tcttcctgag gatcgtgttg 1020 atgtgactgc gtactttgac ccagtcaaga ccacaaagga caaaatctac tgcaagagag 1080 gaggtttcat tcctgagtat gactttgatg cgcgtgagtt tggtctcaac atgttccaga 1140 tggaagattc tgatgcgaat cagaccatct cacttctcaa ggtcaaagaa gcacttcaag 1200 atgctgggat tgatgcactt ggaaaggaga agaaaaacat aggatgcgtg cttggcattg 1260 gaggtggaca gaagagttca catgagttct acagcagact caactatgtg gttgtggaga 1320 aagtcttgag aaagatgggg atgccagagg aagatgtcaa agtggctgtg gagaagtaca 1380 aagcgaactt cccagaatgg agacttgact ctttccctgg attccttggg aatgtcactg 1440 ctggcagatg caccaacaca ttcaacttgg atggcatgaa ctgcgttgtg gatgctgcat 1500 gtgcaagctc cttgatagca gtcaaggttg cgattgatga actgctctat ggtgactgcg 1560 acatgatggt cactggtgca acctgcacag acaactccat tgggatgtac atggctttct 1620 caaagacacc tgtgttcagc acggacccaa gtgtgagagc gtatgatgag aaaacgaaag 1680 ggatgctcat tggtgaggga tcagccatgt tggtgctcaa acgttatgca gatgcagtga 1740 gggatggtga tgagattcat gctgtgattc gtggttgtgc aagctcaagt gatggcaaag 1800 cagctggcat ctacactccc acaatcagtg gtcaagagga agccttgaga cgtgcataca 1860 atcgtgcctg cgttgaccct gccactgtca ctttggttga gggtcatggg actggcactc 1920 cagttggaga taggattgag ttgactgcgt tgaggaactt gtttgacaaa gcgtatggtg 1980 aagggaacac agagaaagtt gcagttggaa gtatcaagtc cagtattggt catttgaagg 2040 ctgttgctgg tcttgctgga atgatcaagg tcatcatggc tctcaaacac aagactttgc 2100 ctggaacgat caatgtggac aatccaccca atctctatga caacactccc atcaatgagt 2160 catccctcta catcaacacg atgaacagac cctggttccc tccacctggt gttccgagac 2220 gtgctggaat atccagcttt ggctttggag gtgccaacta ccatgcagtg ttggaagagg 2280 ctgaaccaga gcacacgaca gcgtacagac tcaacaaacg tccacaaccg gtgttgatga 2340 tggctgcaac tcctgctgcg ttgcagagcc tttgtgaagc tcagctgaaa gagtttgaag 2400 ctgcgatcaa ggagaatgag acagtcaaga atactgccta catcaagtgt gtcaagtttg 2460 gagaacagtt caagttccct gggagcatac ctgcgaccaa tgcgagactg ggattcttgg 2520 tcaaagatgc ggaggatgcg tgctccacgc ttcgtgctat atgtgctcag tttgccaaag 2580 atgtgaccaa agaagcgtgg aggcttccga gagaaggagt ttctttccgt gccaaaggca 2640 ttgccaccaa tggtgctgtt gctgcactct tctctggaca aggtgctcag tacacacaca 2700 tgttcagtga agttgcgatg aactggcctc agttcagaca gtccattgct gcaatggacg 2760 ctgcacagtc caaggttgct ggaagtgaca aagactttga aagagtctct caagtcctgt 2820 acccaaggaa accgtacgaa cgtgagccag aacaagatca caagaaaatc tcactcacag 2880 catacagcca gccgtcaacc ctggcatgtg ctcttggtgc ctttgagatt ttcaaggaag 2940 ctggattcac tccagacttt gcagctggac acagcttggg tgagtttgct gcgctctatg 3000 cagctggttg tgttgacaga gatgagttgt ttgaacttgt ttgcagaagg gcaaggatca 3060 tgggaggcaa agatgcacct gccacaccca aaggttgcat ggcagctgtc ataggaccca 3120 acgcagagaa catcaaggtt caagctgcca acgtctggct tggaaactca aactctccgt 3180 ctcagactgt cattactggg tctgttgaag ggattcaagc tgaaagtgcc agattgcaga 3240 aggaagggtt cagagttgtg cctcttgctt gtgagtctgc cttccattct ccacagatgg 3300 aaaatgccag ctcagctttc aaagatgtca tatccaaggt ttccttccgt acacccaaag 3360 cagaaacaaa gctcttcagc aatgtttctg gagagaccta tccgacggat gccagagaga 3420 tgctcactca acacatgacc tcatctgtga agttcctgac ccaagtgagg aacatgcacc 3480 aagctggtgc gagaatcttt gttgagtttg gaccgaaaca agttctcagc aaacttgttt 3540 ctgaaaccct caaagatgac ccgtctgttg tcacagtgtc tgtgaaccca gcttctggca 3600 cggactctga catacagttg agggatgctg cagttcagct ggttgtggct ggtgtcaatc 3660 ttcaagggtt tgacaagtgg gatgctcctg atgccacgag gatgcaagcc atcaagaaaa 3720 agagaaccac actcagactt tcagctgcca cctatgtcag tgacaagacc aagaaagtga 3780 gggatgctgc catgaacgac ggaagatgtg tcacatacct caagggtgct gcaccactca 3840 tcaaggctcc tgaacctgtg gttgacgaag ctgccaagag ggaggctgaa aggcttcaga 3900 aggagcttca agacgctcag aggcagcttg acgacgccaa acgtgcagct gcagaagcca 3960 actccaaact tgctgctgcc aaggaggaag ccaagactgc tgcagcttca gccaagcctg 4020 cagttgacac tgctgttgtg gagaaacatc gtgccatcct caagtccatg ctggctgagc 4080 tggatggtta tggaagtgtt gatgcttctt cccttcagca acagcaacag caacagactg 4140 ctccggcgcc tgttaaagcc gctgcccctg ccgctcccgt tgcaagtgct cctgctcccg 4200 ccgtgtccaa cgagttgctt gagaaagctg agacagttgt gatggaggtt ctcgctgcca 4260 agactggata cgaaacagac atgattgagg cggacatgga gcttgagact gagttgggaa 4320 ttgacagcat caagagggtt gaaattcttt ctgaagtcca agctatgctt aacgttgagg 4380 cgaaagatgt tgatgctctc tctaggacga gaacagtggg tgaagttgtc aacgctatga 4440 aggcagaaat cgctggttct tccgctccgg cgccggcagc tgccgcacct gcaccggcta 4500 aagcggctcc agctgcagct gcaccagctg tttctaatga gctgctcgaa aaggcagaaa 4560 ctgttgtgat ggaagtcctt gctgccaaaa ctggctacga aactgacatg atagaatcag 4620 acatggagct tgagacggag cttggaattg attccatcaa gcgtgtggaa atcctcagtg 4680 aagtccaagc catgctcaat gtggaagcta aggatgtgga tgctctctca aggaccagaa 4740 ctgtcggtga ggttgtcaat gccatgaagg ctgagatagc tggaggctcc gctcctgcgc 4800 cggccgctgc cgctcctggt cccgctgccg cggctccagc tccagctgca gccgctccgg 4860 ccgtctcaaa cgaactcctt gaaaaagctg agaccgtcgt gatggaggtc ttggcagcta 4920 aaaccggcta tgagactgac atgattgagt ctgacatgga attggagaca gaacttggga 4980 tagacagcat caaacgtgtt gaaatcctca gtgaggtcca agctatgctg aacgtggaag 5040 cgaaggatgt tgatgctctg tccagaacga ggaccgttgg tgaggttgtg gatgccatga 5100 aggctgaaat tgctggaggg tctgcgcccg cgccagctgc agccgctcct gcacccgcag 5160 ccgcggcacc tgctccagct gcacctgcac ctgccgtttc ttcagaactc ttggagaagg 5220 ctgagacggt tgtgatggag gttttggctg caaagactgg ctatgagacc gacatgattg 5280 aatctgatat ggaattggag acagagcttg ggatcgattc catcaaaaga gtggagatcc 5340 tgagtgaagt ccaagccatg ctcaatgttg aagctaagga tgttgatgca ctttcaagaa 5400 caaggacagt gggtgaggtt gtggatgcca tgaaggcgga gattgctgga gggagcgcgc 5460 ctgcgccagc tgccgcggca cctgcaccgg cagccgcagc tccagctcca gctgcaccag 5520 caccggccgc gcccgcgcca gctgtttcat ccgaactcct ggaaaaggcc gagacggttg 5580 tgatggaggt gctcgcagct aagactggtt acgagaccga catgattgaa agtgacatgg 5640 agcttgaaac cgagttggga atagatagca tcaaacgtgt tgaaatcttg tctgaggtcc 5700 aagctatgtt gaacgtggag gcaaaagatg tcgatgcgct ttcaagaacc agaacagtcg 5760 gtgaggtcgt ggacgccatg aaggctgaaa ttgccggaag ctctgctagt gcgccagctg 5820 cagccgcacc tgcacctgct gccgcggcac ctgctccagc tgcagccgct cctgccgtca 5880 gcaatgagtt gctggagaaa gccgaaacag ttgtgatgga agtccttgct gcaaaaactg 5940 gttacgaaac ggacatgatt gagtcagaca tggaattgga gaccgaactt ggaatagatt 6000 ccatcaagag agtggagatc ctctctgagg tgcaagccat gctcaacgtt gaggctaaag 6060 atgttgatgc actcagtagg acaagaactg tgggggaagt tgtcgatgcg atgaaggctg 6120 agattgctgg aggctctgct cctgcgccgg cagctgccgc acctgcaccc gcagccgcgg 6180 caccagctgt cagcaacgaa ttgcttgaga aggctgaaac agttgtcatg gaagtgctgg 6240 ctgcaaaaac cggctatgag actgacatga ttgagtctga catggaactt gagacggaat 6300 tgggcattga cagcatcaag cgtgttgaga tcctcagtga agtccaagca atgctcaatg 6360 tggaggctaa ggatgtggat gccctctcaa ggaccagaac agttggtgag gtcgttgatg 6420 cgatgaaggc ggagatcgct ggaagttccg cgcctgcgcc agctgcagcc gctcctgcac 6480 ccgcagccgc ggcaccggct ccagctgcag ccgcaccggc cgtctcctca gaactcctgg 6540 aaaaggctga gacagttgtg atggaagtcc ttgctgcgaa aactggctat gagaccgaca 6600 tgattgaatc tgacatggaa ctggagaccg agcttggaat tgactccatc aaacgtgttg 6660 aaatcctctc tgaggttcaa gcgatgttga atgtggaggc caaagatgtt gatgctcttt 6720 ccagaacaag gacggtggga gaggtggttg atgccatgaa ggctgagata gctgggggtt 6780 ctgctcctgc gccagctgca gccgctcctg ctcccgctgc cgcggcacca gctgtcagca 6840 acgaactcct tgaaaaagct gaaactgttg tgatggaggt tttggctgca aagactggat 6900 acgagactga catgatagag tcagacatgg agttggaaac agagcttggg attgatagca 6960 tcaagcgtgt ggaaatcctt tctgaggttc aagccatgct gaacgttgag gccaaagatg 7020 tcgatgcttt gtcaaggacc agaacggttg gagaagtggt cgatgccatg aaggctgaga 7080 tagctggtgg atcagcccct gctccggctg cagccgctcc tgcaagtgcc ggtgctgcac 7140 cagctgtgaa gattgattca gttcatggtg ctgactgtga cgatttgtca ctgatgcacg 7200 caaaggtggt tgacatcaga aggccagatg aactgattct ggaaagaccg gagaaccgtc 7260 cagttcttgt cgttgacgat ggctcagagt tgacacttgc tcttgtgagg gtgttgggag 7320 cctgtgcagt tgtcttgacc tttgagggat tgcagttggc tcagagagct ggtgcagctg 7380 ccattcgtca tgttctggct aaggaccttt cagcagaatc agctgagaag gccatcaaag 7440 aggctgagca aagatttgga gcgttgggag gcttcataag ccagcaagca gaaaggtttg 7500 aaccagctga gatactgggt ttcacactca tgtgtgcgaa gtttgccaaa gcctctctct 7560 gcactgctgt ggctggaggt cgtcctgcct tcatcggtgt ggctaggctt gacggcagac 7620 ttggattcac ctctcaaggc acttctgacg cactcaagag agcacagcgt ggagcgatct 7680 ttggactctg caagacaata gggttggagt ggtcagaatc tgatgtgttc agccgtggtg 7740 tggacattgc tcaagggatg cacccagaag atgctgcagt tgccatagtt agggagatgg 7800 cgtgtgctga catcagaatc agagaggttg ggattggagc aaaccagcaa agatgcacaa 7860 tcagagctgc caagttggag actgggaacc ctcagagaca gatagcgaaa gacgatgtgc 7920 ttctggtttc tggaggtgct cgtgggatca cacctctttg catccgtgag atcacgcgtc 7980 agattgctgg aggcaagtac attctgttgg gtcgtagcaa agtttctgcg tctgaaccag 8040 catggtgtgc tggcatcact gatgagaagg ctgtgcagaa agctgccacc caagagttga 8100 agagggcctt cagtgctggt gaaggaccca aacccactcc aagggctgtc acgaaacttg 8160 tgggatctgt tcttggtgct cgtgaagtga gaagctccat tgctgccatt gaagctctgg 8220 gtggcaaagc gatctacagc tcttgtgatg tgaactctgc tgcagatgtt gccaaagcag 8280 ttagggatgc agagtcccag cttggtgcca gagttagtgg aatcgttcac gcttctggtg 8340 tgctccgtga tcgtctcata gagaagaaac tccctgacga gtttgatgct gtctttggga 8400 ccaaagtcac tgggttggag aatcttctgg ctgcagttga tcgtgccaac ctcaaacaca 8460 tggttctctt cagctccctt gctggtttcc acggcaatgt tggtcagtct gactatgcga 8520 tggcaaacga agctctcaac aagatgggtt tggaacttgc gaaggatgtc agtgtcaagt 8580 ccatctgctt tggtccctgg gatggtggga tggtgacacc tcaactcaag aaacagtttc 8640 aagaaatggg agttcagatc attcctcgtg aaggaggtgc tgatacagtt gcgaggattg 8700 tgttgggatc aagtcctgct gagatccttg ttggaaactg gaggacacca tccaagaaag 8760 ttggaagtga cacgatcacc ctgcatagaa agatcagtgc caagtccaat cccttcttgg 8820 aggatcatgt catacaaggc agaagggtgt tgccaatgac ccttgctatt gggagccttg 8880 ctgagacctg ccttggactc ttccctggct actctctctg ggccatagat gacgcacagc 8940 tcttcaaagg tgtcactgtt gatggagacg tcaactgtga agtgaccctc acaccgtcca 9000 ctgctccgag tgggagggtg aatgtccaag cgacgctcaa gacgttctca agtggaaaac 9060 ttgttccagc ctacagagct gtcatagtgt tgtccaacca aggtgctcca cctgccaatg 9120 caaccatgca gcctccgagc cttgatgcag atcctgcttt gcaagggtca gtctatgatg 9180 gcaagactct ctttcatggt cctgcgttca gaggcattga tgacgttctc agctgcacca 9240 agagccagct tgttgccaag tgttctgctg ttcctggatc agatgcagcc agaggagagt 9300 ttgcaactga cactgatgca catgatccct ttgtcaatga tcttgccttt caagcaatgt 9360 tggtctgggt gaggagaacg ctgggacaag ctgcactccc gaactccatt cagaggatag 9420 tccaacacag accggtccca caagacaaac ccttctacat caccctcaga tccaatcaga 9480 gtggtggaca tagccagcac aaacatgcac ttcagttcca caacgaacaa ggtgatctct 9540 tcattgatgt tcaagcctct gtcattgcga cagactcact tgcgttctga gtagttagct 9600 taatcactta ggtcacccta tcaaaatcta tttagaaata cacaatattt tgttgcaggc 9660 ttgctggaga atcgatctgc tatcataaaa attacaaaaa aattttattt gcctcaatta 9720 ttttaggatt ggtattaagg acgcttaaat tatttgtcgg gtcactacgc atcattgtga 9780 ttgagaagat cagcgatacg aaatattcgt agtactatcg ataatttatt tgaaaattca 9840 taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata aagccacgca 9900 catttaggat attggccgag attactgaat attgagtaag atcacggaat ttctgacagg 9960 agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc catatgcagg 10020 agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca aggttgcggc 10080 cgcttaatta agatgagtga tactcaggac tcaggactca ctctgctgat cactagtgct 10140 agcctcgagg tcgacacaac tttgtataca aaagttgtct agacctaggc tcagcaactc 10200 atccagtcaa gttcgagtca gtgagttggg cggccgcaga tctgattggt aagatatggg 10260 tactgtttgg tttatatgtt ttgactattc agtcactatg gcccccataa attttaattc 10320 ggctggtatg tctcggttaa gaccggtttg acatggttca tttcagttca attatgttaa 10380 tctggcacgt gatatgttta ccttcacacg aacattagta atgatgggct aatttaagac 10440 ttaacaggcc tagagaggcc catcttatta cgtaacggcg tcgtttagag tgcaccaagc 10500 ttataaatga aaacgagcta cctcgggaca tcacgctctt tgtacactcc gccatctctc 10560 tctctcgttg gagcagatct ctctcgtgaa tatcgacaat ggctgcaagg aacgtgagtg 10620 cggctcatga gatgcatgac gagaagagga tagctgttgt gggcatggct gttcagtatg 10680 ctggatgcaa aacgaaagac gagttctggg aagttctcat gaatggaaaa gttgagagca 10740 aagtgatcag cgacaaacgt cttggttcaa attaccgtgc tgaacactac aaagctgaaa 10800 ggagcaagta tgctgacacc ttctgcaatg agacatatgg aactcttgat gagaacgaaa 10860 tagacaatga gcatgaactc cttttgaacc ttgccaaaca agccttggct gaaacatctg 10920 tcaaagattc aacaagatgt ggcatagttt ctggatgcct cagcttcccg atggacaacc 10980 tccaaggtga actcttgaat gtgtaccaga accatgttga gaagaaactt ggtgccagag 11040 ttttcaaaga tgcctctcac tggtcagaaa gagaacagtc caacaaacca gaggctggtg 11100 acagaagaat cttcatggac cctgcttcct ttgttgctga ggaactcaat cttggcgctc 11160 tccactactc tgttgatgct gcctgtgcca ctgcgctcta tgtgctcaga ctggcgcaag 11220 atcacttggt ctctggtgca gctgatgtga tgctttgtgg tgcaacatgc ctgccggagc 11280 cgtttttcat actctctggg ttctcaacct tccaagccat gcctgttgga acgggtcaga 11340 atgtgtccat gccgctgcac aaagattctc aaggattgac ccctggtgaa ggaggttcca 11400 tcatggtcct caaacgtttg gatgacgcaa tcagagatgg agatcacatc tatggaacac 11460 ttttgggagc caatgtcagc aacagtggca ctggtctgcc acttaaaccc cttttgccgt 11520 cagagaaaaa gtgcctcatg gacacctaca cacgtatcaa tgtgcatccc cacaagattc 11580 agtatgttga atgccatgca actgggactc cacaaggtga tcgtgtggaa attgatgctg 11640 tgaaggcttg ctttgaaggc aaagtcccac gttttggaac cacaaaaggg aactttggac 11700 acacattggt ggcagctggg tttgctggca tgtgcaaagt gcttctctcc atgaagcacg 11760 ggatcattcc acccactccg ggaatagatg acgaaacgaa gatggaccct ctggttgtct 11820 ctggtgaggc cattccctgg ccagaaacga atggagaacc gaagcgtgct ggtctctcag 11880 cgtttggatt tggtgggacc aatgctcatg ctgtctttga ggaacatgac ccaagcaatg 11940 ctgcatgcac tggccatgac tccatttctg ctctctctgc acgttgtggt ggtgagtcaa 12000 acatgagaat agcaatcact gggatggatg ccacttttgg tgctttgaaa ggattggatg 12060 cttttgaaag ggccatctac actggtgctc atggtgctat accactccca gaaaagagat 12120 ggagattcct ggggaaagac aaggacttcc ttgatctttg tggagtcaaa gcaacaccgc 12180 atggctgtta cattgaagat gttgaagttg atttccagag gctgaggact ccaatgactc 12240 ctgaagatat gctcttgcct caacagcttc tggctgtcac cacaattgat agggccatat 12300 tggactctgg catgaagaaa ggtggaaatg tggctgtctt tgtggggttg gggaccgatc 12360 ttgaactcta ccgtcaccgt gcaagggttg ctctcaagga gagagtgagg cctgaagcca 12420 gcaagaaact caatgacatg atgcagtaca tcaatgattg tgggacaagc acttcttaca 12480 ccagctacat aggcaacttg gttgcaacaa gggtcagttc acaatggggc ttcacgggac 12540 cttccttcac aatcacagaa ggcaacaatt ctgtgtacag atgtgctgaa cttggcaaat 12600 acctcttgga aacgggagag gttgatgggg ttgtggttgc gggtgttgat ctctgtggga 12660 gtgctgagaa cctttacgtc aagtcaaggc gtttcaaggt gtctacatct gacaccccga 12720 gagccagctt tgatgcagct gctgatggtt actttgttgg agagggctgt ggtgcatttg 12780 tgctcaagcg tgagacaagc tgcaccaagg atgacagaat ctatgcttgc atggatgcca 12840 tagttcctgg aaatgttcca tctgcatgtt tgagagaggc attggatcaa gccagagtca 12900 agcctggaga catcgagatg ttggagcttt ctgctgattc agcgaggcat ttgaaggacc 12960 cgtctgttct cccaaaggaa ctcactgctg aggaagagat tggcggactt cagacgattc 13020 ttcgtgacga tgacaagctc ccaaggaatg ttgcaactgg aagtgtcaag gccactgttg 13080 gtgacactgg ttatgcttct ggtgctgcca gtttgattaa ggctgcgctt tgcatctaca 13140 accgttacct gcccagcaat ggagatgact gggatgaacc tgcacctgaa gcaccctggg 13200 acagcacact ctttgcgtgc cagacctcaa gagcttggtt gaagaaccct ggtgagagac 13260 gttatgcggc tgtttccgga gtcagtgaaa ccagaagttg ctacagtgtg ctgctttctg 13320 aagcagaggg acactacgag agagagaaca gaatcagttt ggatgaggaa gcacccaagc 13380 tcattgtgtt gagagcagac tcccatgaag agattcttgg aagattggac aagataaggg 13440 aaaggttctt gcagcccacg ggtgctgcac ccagagagtc tgaattgaag gcgcaagccc 13500 gtaggatctt tcttgaactc cttggtgaga cccttgccca agatgcagct tcatctggaa 13560 gtcagaagcc ccttgctctc agcttggtct ccactccatc caagctccag agagaggtgg 13620 aacttgcggc aaaaggcatt ccgagatgtc tcaagatgag acgtgattgg agttcccctg 13680 ctggaagcag atatgcgcct gagcctttgg ccagtgacag agttgccttc atgtatggag 13740 aaggaaggag cccttactat ggcatcacac aagacattca taggatttgg cctgaacttc 13800 atgaggtcat caatgagaaa acaaaccgtc tctgggctga aggtgacaga tgggtcatgc 13860 caagggccag cttcaagagt gagcttgagt cacaacagca agagtttgac agaaacatga 13920 ttgaaatgtt ccgtcttgga atcctcacct caattgcctt cacaaacctg gcgagagatg 13980 ttctcaacat cacgcccaaa gcagcctttg gactcagttt gggagagatt tccatgatct 14040 ttgctttctc caagaaaaac ggtttgataa gcgatcagtt gacaaaggat ttgagagagt 14100 cagatgtctg gaacaaagct ctggctgttg agttcaatgc gctcagagaa gcctggggca 14160 ttccacagtc tgtcccaaaa gatgagttct ggcaaggtta catagtgagg ggaacgaaac 14220 aagacataga agcagccata gcaccagact caaagtatgt gaggttgacc atcatcaatg 14280 atgccaacac tgctctcatc agtggaaagc ctgatgcatg caaagctgcg attgctcgtc 14340 ttggtggcaa catccctgcg cttccagtca ctcaaggcat gtgtgggcat tgcccagagg 14400 tgggacctta caccaaggac atcgcaaaga tccacgccaa ccttgagttc cctgtggttg 14460 atggtcttga tctctggact accataaacc agaagaggct tgtgccaaga gccacgggtg 14520 ccaaagatga atgggctcct tcatcctttg gggagtatgc tggacagctc tatgagaaac 14580 aagccaactt ccctcagata gttgaaacca tctacaagca gaactatgat gtgtttgttg 14640 aagttgggcc aaacaatcac agaagcacag cagtgagaac cacgcttgga ccacagagaa 14700 accaccttgc tggtgccata gacaagcaga acgaggatgc ttggacgaca atagtcaagc 14760 tcgtggcttc attgaaggct caccttgttc ctggagtgac catcagtccg ctctaccact 14820 ccaagctcgt tgcagaggct gaggcttgtt atgctgcgct ctgtaaaggt gagaaaccca 14880 agaaaaacaa gtttgtgaga aagattcagt tgaatggcag attcaacagc aaggcagacc 14940 ccatctcctc agcagacctt gcatccttcc cacctgcgga ccctgcgatt gaagctgcca 15000 tctcttcaag gatcatgaag cctgtggctc cgaaattcta tgcgcgtctc aacattgatg 15060 agcaagatga gaccagagat ccgatcttga acaaggacaa tgcgccttcc agctccagct 15120 cttccagcag ttcctcttcc agcagttcat ctccgtctcc tgctccttca gctccagttc 15180 agaagaaagc tgccccagct gcggagacaa aagctgttgc cagtgctgat gcacttcgta 15240 gtgccctgct tgatcttgac tccatgcttg cgctcagctc tgcaagtgct tctggcaacc 15300 ttgttgagac tgcgccatct gatgccagtg tcattgtccc tccgtgcaac attgctgatc 15360 ttggcagcag agctttcatg aaaacttatg gtgtttctgc gcctctgtac accggtgcaa 15420 tggccaaggg aattgcctct gcggacttgg tgatcgcagc tggaaggcaa ggaatccttg 15480 cgtcatttgg tgctggaggc cttccaatgc aagttgtgcg tgagtccatt gaaaagattc 15540 aagctgccct gcccaatgga ccatatgctg tcaacctcat ccattctcct tttgacagca 15600 accttgagaa gggaaatgtt gatctcttct tggaaaaggg tgtcactttt gttgaggctt 15660 cagccttcat gacgttgaca ccgcaagttg tcagatacag agcggctggg ttgacgagga 15720 atgcagatgg gtcagtcaac ataaggaaca gaatcattgg gaaagtctcc agaactgagc 15780 tggctgaaat gttcatgcgt cctgcacctg agcatctttt gcagaagctc attgcttctg 15840 gggagatcaa tcaagaacaa gctgagttgg ccagacgtgt tcctgtggct gatgacattg 15900 cggttgaagc tgattctggt ggacacactg acaaccgtcc aatccatgtc attctgcctc 15960 tgattatcaa cctgagggat aggcttcaca gagagtgtgg atacccagca aacctgaggg 16020 ttcgtgttgg tgctgggggt ggcattgggt gtccccaagc tgcgctggca actttcaaca 16080 tgggtgcctc tttcattgtc actggaacag tgaaccaagt tgccaaacaa tctgggacgt 16140 gtgacaatgt gaggaaacag cttgcgaaag caacttactc agatgtttgc atggcaccgg 16200 cagctgacat gtttgaggaa ggtgtgaaac ttcaagtttt gaagaaagga acaatgttcc 16260 cttcaagagc caacaaactc tatgagttgt tctgcaagta tgattctttt gagtccatgc 16320 ctccagcaga gcttgcgagg gttgagaaaa gaatcttcag tcgtgcattg gaagaggtct 16380 gggatgagac caagaacttc tacatcaacc gtctccacaa cccagagaag atacagaggg 16440 ctgagaggga ccccaaactg aagatgtcac tgtgtttcag atggtatctg tccctggctt 16500 caaggtgggc caacactgga gcttctgaca gagtcatgga ctaccaagtt tggtgtggtc 16560 ctgccattgg ttcattcaat gatttcatca aaggaactta ccttgatccg gcagtggcaa 16620 atgagtaccc gtgtgttgtg cagatcaaca aacagattct tagaggagcg tgcttcttga 16680 ggcgtcttga aatactgaga aatgcaaggc tttcagatgg tgcagctgca cttgtggcga 16740 gcattgatga cacatatgtc cctgctgaga agctgtgagt agttagctta atcacttagg 16800 tcaccctatc aaaatctatt tagaaataca caatattttg ttgcaggctt gctggagaat 16860 cgatctgcta tcataaaaat tacaaaaaaa ttttatttgc ctcaattatt ttaggattgg 16920 tattaaggac gcttaaatta tttgtcgggt cactacgcat cattgtgatt gagaagatca 16980 gcgatacgaa atattcgtag tactatcgat aatttatttg aaaattcata agaaaagcaa 17040 acgttacatg aattgatgaa acaatacaaa gacagataaa gccacgcaca tttaggatat 17100 tggccgagat tactgaatat tgagtaagat cacggaattt ctgacaggag catgtcttca 17160 attcagccca aatggcagtt gaaatactca aaccgcccca tatgcaggag cggatcattc 17220 attgtttgtt tggttgcctt tgccaacatg ggagtccaag gttgcggccg cttaattaat 17280 ctgacctgac cactagacct gagtgagtac agatgtgcta ctagtgctag cctcgaggtc 17340 gaccaacttt tctatacaaa gttgtctaga cctaggacta ggcactgagt gactcactga 17400 cctgactgag tgactggcgg ccgcgcggcc gcgaattcgg aaatgggcca agtgaaatgg 17460 aaatagagct tcaatccatt tagtcccact caaaatggtg ctcgaattat atttagttac 17520 gttcgaatca gacaaccaag tatttggtta ataaaaacca ctcgcaacaa aggaaaaaca 17580 ccaagcgcgt gcgtccaaca tccgacggaa ggggggtaat gtggtccgaa aaccttacaa 17640 aaatctgacg tcatctaccc ccgaaaacgt tgaatcgtca acgggggtag ttttcgaatt 17700 atcttttttt taggggcagt tttattaatt tgctctagaa attttatgat tttaattaaa 17760 aaaagaaaaa gaatatttgt atatttattt tttatactct ttttttgtcc aactatttct 17820 cttattttgg caactttaac tagactagta acttatgtca atgtgtatgg atgcatgaga 17880 gtgagtatac acatgtctaa atgcatgcct tatgaaagca acgcaccaca aaacgaagac 17940 ccctttacaa atacatctca tcccttagta ccctcttact actgtcccga cacaaactca 18000 aaacaaggat ccacaaccaa aaatggcgct tcgtgtcaag accaacaaga agccatgttg 18060 ggagatgacc aaggaggaac tgacctctgg caaaactgag gtgttcaact atgaggaact 18120 cctggagttt gcagagggtg acatagctaa ggtttttggt ccggagtttg ctgtcataga 18180 caagtacccc agaagggtga ggctgcctgc tcgtgaatac ctgcttgtga caagggtcac 18240 actcatggat gccgaggtca acaactacag agttggtgcc agaatggtca ctgagtatga 18300 tcttccagtg aatggcgaat tgtctgaggg aggtgattgt ccctgggcag ttctggttga 18360 gagtggacag tgcgatttga tgctgatatc atacatggga attgatttcc agaaccaagg 18420 tgacagagtt taccgtctgc tcaacaccac gttgacattc tatggggttg cccatgaggg 18480 agagaccctt gagtatgaca tcagagtgac cggttttgcc aaacgtttgg atggtggcat 18540 ctccatgttc ttctttgagt atgactgcta tgtcaatggg aggcttctca ttgagatgag 18600 agatggctgt gctggcttct tcacaaatga ggagcttgat gcgggaaagg gtgtggtgtt 18660 cacaagaggg gatttggctg ccagagctaa gattccaaag caagatgttt ctccctatgc 18720 tgtcgctccc tgccttcaca aaacaaaact caacgaaaag gagatgcaga ccttggttga 18780 caaagattgg gcttccgttt ttggctcaaa gaatggcatg ccggagatca actacaaact 18840 ctgcgcacgt aagatgctca tgattgatag agtcaccagc attgaccata agggaggtgt 18900 gtacggtttg ggtcagttgg ttggtgagaa aatcttggag agggaccatt ggtacttccc 18960 atgccatttt gttaaggatc aagtcatggc tggatctctt gtcagcgacg gttgttcaca 19020 aatgctcaag atgtacatga tctggctcgg tttgcacctt accaccggac catttgactt 19080 cagacccgtc aacggacacc cgaacaaagt taggtgtagg ggtcagatat ctcctcataa 19140 gggaaagttg gtctacgtca tggagatcaa agagatggga ttcgacgagg ataacgatcc 19200 gtacgccatt gctgacgtca acatcattga cgttgatttc gaaaagggtc aagatttcag 19260 ccttgataga atcagcgact acggaaaggg ggatcttaac aaaaagatcg tcgtggactt 19320 caagggcatt gctctcaaga tgcagaagag atccacgaac aagaacccct ccaaagttca 19380 gcccgttttt gctaacggtg cagccactgt gggaccggaa gcgtccaagg ccagctctgg 19440 tgccagtgct agtgcttctg ctgcccctgc gaagcctgct ttctcagcag atgttcttgc 19500 gccaaagcca gttgcacttc ctgaacatat cctcaagggt gacgctctcg ctccgaaaga 19560 aatgtcctgg catcccatgt caaaacttcc tggcaacccg acgccgttct tctcccctag 19620 ttcttaccct ccgagggcaa tttgcttcat ccctttccct ggcaacccac ttgacaacaa 19680 ttgcaaagct ggagaaatgc ctctgaactg gtacaacatg tcagagttca tgtgtggaaa 19740 agttagcaac tgcttgggac cagagttcgc aagatttgat aagtcaaata cgagcagaag 19800 ccctgctttt gatttggctc ttgtgactag agttgttgaa gtcacaaaca tggaacatgg 19860 caagttcctg aatgttgatt gcaatcccag caaaggcaca atggtggggg agtttgactg 19920 tcctcaagat gcgtggttct ttgatggttc ttgcaatgat gggcacatgc cttattccat 19980 catcatggaa atcggacttc agacatccgg tgttctcacc agtgtgttga aagcaccact 20040 gacgatggac aaagatgaca ttctctttag gaaccttgat gcaagtgctg aaatggtgag 20100 gccagatgtg gatgtgaggg ggaaaaccat aaggaatgtg accaagtgca ctggatatgc 20160 aatgttggga aagatgggga ttcacagatt cacgtttgag ttgtctgttg atggcgtggt 20220 tttctacaaa ggaagcactt cctttggatg gttcactcca gaggtgtttg ctcagcaagc 20280 tggacttgac aatgggaaaa agacggagcc ttggtgcaaa actaacaaca cgagtgtgag 20340 aagagttgaa atcgcatcag ccaaaggcaa agaacagctg actgagaaac ttccagatgc 20400 aactaatgct caagttctca gaaggtcaga acagtgtgag tatttggatt acctcaacat 20460 tgctcctgat tctgggctgc atgggaaagg atatgcgcat ggacacaaag atgttaaccc 20520 acaagactgg ttcttctctt gtcacttttg gtttgatcct gtgatgcctg gatctttggg 20580 aattgagtca atgttccagc tcattgaggc atttgcggtg gaccagaaca taccgggaga 20640 atacaatgtg agcaatccga cctttgccca tgcccctgga aaaacggcgt ggaagtaccg 20700 tggtcaattg acaccaaaga accgtgcgat ggactgtgag gtgcacatag tttcaatcac 20760 agcctctcct gaaaatggag gctacgttga catagtggca gatggagcac tttgggttga 20820 tggtctccgt gtgtatgaag ccaaagaact cagagttcgt gttgtcagtg caaaacctca 20880 agccattccg gatgttcaac aacagccacc cagcgcaaaa gcggaccctg ggaaaactgg 20940 agttgcactc agtccgactc agttgagaga tgtcctgctt gaagtggaca atccattgta 21000 tcttggtgtt gaaaactcca atttggtgca gtttgaatcc aaacctgcaa cttcttcaag 21060 gattgtcagt atcaaaccat gctccataag tgatcttggt gacaagtcct tcatggaaac 21120 gtacaatgtg tcagcacctc tgtacactgg agcaatggcc aagggaattg cgtctgcgga 21180 ccttgtcatt gctgctggga agaggaagat attgggttct tttggagcgg gtgggcttcc 21240 aatgcaccat gtgagggcag ctttggagaa gatacaagcg gctctgcctc aaggaccata 21300 tgctgtgaac ctcattcatt caccttttga cagcaacttg gaaaagggga atgttgatct 21360 cttcctcgaa aagggcgtta ctgtggtgga ggcttctgca ttcatgaccc tcactcccca 21420 agttgttcgt taccgtgcag ccggactctc aaggaacgct gatgggagtg tcaacatcag 21480 aaaccgtatc attggaaaag tgtccagaac agaactcgcc gaaatgttca tcagaccggc 21540 tccggaacac ctccttgaga aactcatagc ttctggtgag ataacacaag aacaagccga 21600 acttgcccgt cgtgttcctg tcgcagacga catcgctgtt gaagctgatt ctggtggtca 21660 cactgacaac cgtcccatcc acgtcatctt gcctttgatc atcaacctta ggaacagact 21720 gcacagagag tgtggttatc ctgcgcacct tagagtcaga gttggagctg gaggcggtgt 21780 tggctgccca caagctgctg cggctgccct cacaatggga gctgccttca tagtgactgg 21840 gactgtcaat caagttgcta aacagtccgg gacatgtgac aacgtgagaa aacaactcag 21900 tcaagccacc tacagtgaca tttgcatggc tccagcagct gatatgtttg aggaaggtgt 21960 gaaactccaa gttctcaaga agggaacaat gttcccatca agagctaaca aattgtacga 22020 actcttttgc aagtacgact ctttcgattc aatgcctcct gctgaacttg aaaggatcga 22080 gaagaggatc ttcaagaggg cacttcaaga ggtctgggaa gagaccaagg acttttacat 22140 caatggtttg aagaatccag agaagataca gagagcggaa catgacccca aactcaagat 22200 gtctttgtgt ttcagatggt atcttggtct tgcatcacgt tgggccaaca tgggtgctcc 22260 tgatcgtgtc atggactatc aagtctggtg tggtccagcc attggagcct tcaatgactt 22320 catcaaagga acatatcttg atcctgctgt ctctaatgag tacccatgtg ttgtccagat 22380 caaccttcag attttgcgtg gtgcatgtta cctgaggcgt cttaatgctc tgagaaatga 22440 tccgaggatt gatcttgaga ctgaagatgc tgcctttgtc tatgagccaa cgaatgcgct 22500 ttgagtagtt agcttaatca cttaggtcac cctatcaaaa tctatttaga aatacacaat 22560 attttgttgc aggcttgctg gagaatcgat ctgctatcat aaaaattaca aaaaaatttt 22620 atttgcctca attattttag gattggtatt aaggacgctt aaattatttg tcgggtcact 22680 acgcatcatt gtgattgaga agatcagcga tacgaaatat tcgtagtact atcgataatt 22740 tatttgaaaa ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca 22800 gataaagcca cgcacattta ggatattggc cgagattact gaatattgag taagatcacg 22860 gaatttctga caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc 22920 gccccatatg caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag 22980 tccaaggttg cggccgctta attaatcacg agtggatgac tgtcacgact gactcatgac 23040 tgactactag tgctagcctc gaggtcgaca caactttgta taataaagtt gtctagacct 23100 aggagctgtg ctgggtaact cacatgagtc actggtcaca tcagcggccg cagatctgat 23160 tggtaagata tgggtactgt ttggtttata tgttttgact attcagtcac tatggccccc 23220 ataaatttta attcggctgg tatgtctcgg ttaagaccgg tttgacatgg ttcatttcag 23280 ttcaattatg ttaatctggc acgtgatatg tttaccttca cacgaacatt agtaatgatg 23340 ggctaattta agacttaaca ggcctagaga ggcccatctt attacgtaac ggcgtcgttt 23400 agagtgcacc aagcttataa atgaaaacga gctacctcgg gacatcacgc tctttgtaca 23460 ctccgccatc tctctctctc gttggagcag atctctctcg tgaatatcga caatgcttca 23520 gcacacttgg cttccgaagc ctcccaatct gaccctcttg tcagatgagg ttcatctctg 23580 gaggattcct cttgaccagc ctgagtcaca acttcaagac cttgctgcca ccctgagcag 23640 tgatgaattg gcgagggcaa acagattcta ctttccagaa cacagaaggc gtttcactgc 23700 tgggagaggc atcctcagat ccatcttggg tggatacttg ggagtggaac cgggtcaagt 23760 caagtttgat tatgagtccc gtgggaaacc gatccttggt gacagatttg ctgagagtgg 23820 actcctgttc aacttgtctc acagccagaa ccttgccttg tgtgctgtca actacacgcg 23880 tcaaataggc attgatcttg aatatctgcg tccaacatct gacttggagt ctcttgcaaa 23940 gaggttcttt ctcccaagag aatatgaact cttgaggtca ctccctgatg agcagaaaca 24000 gaagattttc tttcgttact ggacttgcaa agaggcttat ctcaaagcaa cgggagatgg 24060 aatagccaaa cttgaagaga tcgagatagc actcacccca acagaacctg ccaagctcca 24120 aacagctcct gcgtggtctc tgttggagct tgtgccagat gacaattgtg ttgcagctgt 24180 ggctgttgcg ggttttggtt ggcagcccaa gttctggcat tactgagtag ttagcttaat 24240 cacttaggtc accctatcaa aatctattta gaaatacaca atattttgtt gcaggcttgc 24300 tggagaatcg atctgctatc ataaaaatta caaaaaaatt ttatttgcct caattatttt 24360 aggattggta ttaaggacgc ttaaattatt tgtcgggtca ctacgcatca ttgtgattga 24420 gaagatcagc gatacgaaat attcgtagta ctatcgataa tttatttgaa aattcataag 24480 aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga cagataaagc cacgcacatt 24540 taggatattg gccgagatta ctgaatattg agtaagatca cggaatttct gacaggagca 24600 tgtcttcaat tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg 24660 gatcattcat tgtttgtttg gttgcctttg ccaacatggg agtccaaggt tgcggccgct 24720 taattaacta ctgtcactga ggccgtagac gagtacggac tgatctaact agtgctagcc 24780 tcgaggtcga cacccagctt tcttgtacaa agtggttgcg gccgcttaat taaatttaaa 24840 ttcaattaat gcaatcttga ttttcaacaa cgaaggtaat ggcgtaaaag aaaaaatgta 24900 tgttattgta ttgatctttc atgatgttga agcgtgccat aatatgatga tgtataatta 24960 aaatattaac tgtcgcattt tattgaaatg gcactgttat ttcaaccata tctttgattc 25020 tgttacatga cacgactgca agaagtaaat aatagacgcc gttgttaaag aattgctatc 25080 atatgtgcct aactagaggg aatttgagcg tcagacctaa tcaaatatta caaaatatct 25140 cactctgtcg ccagcaatgg tgtaatcagc gcagacaaat ggcgtaaaga tcgcggaaaa 25200 acctccccga gtggcatgat agctgcctct gtattgctga tttagtcagc cttatttgac 25260 ttaagggtgc cctcgttagt gacaaattgc tttcaaggag acagccatgc cccacacttt 25320 gttgaaaaac aaattgcctt tggggagacg gtaaagccag ttgctcttca ataaggaatg 25380 tcgaggaggc aatgtaaccg cctctggtag tacacttctc taatccaaaa atcaatttgt 25440 attcaagata ccgcaaaaaa cttatggttt aaaccctgca ggactagtcc agaaggtaat 25500 tatccaagat gtagcatcaa gaatccaatg tttacgggaa aaactatgga agtattatgt 25560 aagctcagca agaagcagat caatatgcgg cacatatgca acctatgttc aaaaatgaag 25620 aatgtacaga tacaagatcc tatactgcca gaatacgaag aagaatacgt agaaattgaa 25680 aaagaagaac caggcgaaga aaagaatctt gaagacgtaa gcactgacga caacaatgaa 25740 aagaagaaga taaggtcggt gattgtgaaa gagacataga ggacacatgt aaggtggaaa 25800 atgtaagggc ggaaagtaac cttatcacaa aggaatctta tcccccacta cttatccttt 25860 tatatttttc cgtgtcattt ttgcccttga gttttcctat ataaggaacc aagttcggca 25920 tttgtgaaaa caagaaaaaa tttggtgtaa gctattttct ttgaagtact gaggatacaa 25980 cttcagagaa atttgtaagt ttgtaggtac cagatctgga tcccaaacca tgtctccgga 26040 gaggagacca gttgagatta ggccagctac agcagctgat atggccgcgg tttgtgatat 26100 cgttaaccat tacattgaga cgtctacagt gaactttagg acagagccac aaacaccaca 26160 agagtggatt gatgatctag agaggttgca agatagatac ccttggttgg ttgctgaggt 26220 tgagggtgtt gtggctggta ttgcttacgc tgggccctgg aaggctagga acgcttacga 26280 ttggacagtt gagagtactg tttacgtgtc acataggcat caaaggttgg gcctaggatc 26340 tacattgtac acacatttgc ttaagtctat ggaggcgcaa ggttttaagt ctgtggttgc 26400 tgttataggc cttccaaacg atccatctgt taggttgcat gaggctttgg gatacacagc 26460 ccggggtaca ttgcgcgcag ctggatacaa gcatggtgga tggcatgatg ttggtttttg 26520 gcaaagggat tttgagttgc cagctcctcc aaggccagtt aggccagtta cccaaatctg 26580 agtagttagc ttaatcacct agagctcgat cggcggcaat agcttcttag cgccatcccg 26640 ggttgatcct atctgtgttg aaatagttgc ggtgggcaag gctctctttc agaaagacag 26700 gcggccaaag gaacccaagg tgaggtgggc tatggctctc agttccttgt ggaagcgctt 26760 ggtctaaggt gcagaggtgt tagcgggatg aagcaaaagt gtccgattgt aacaagatat 26820 gttgatccta cgtaaggata ttaaagtatg tattcatcac taatataatc agtgtattcc 26880 aatatgtact acgatttcca atgtctttat tgtcgccgta tgtaatcggc gtcacaaaat 26940 aatccccggt gactttcttt taatccagga tgaaataata tgttattata atttttgcga 27000 tttggtccgt tataggaatt gaagtgtgct tgaggtcggt cgccaccact cccatttcat 27060 aattttacat gtatttgaaa aataaaaatt tatggtattc aatttaaaca cgtatacttg 27120 taaagaatga tatcttgaaa gaaatatagt ttaaatattt attgataaaa taacaagtca 27180 ggtattatag tccaagcaaa aacataaatt tattgatgca agtttaaatt cagaaatatt 27240 tcaataactg attatatcag ctggtacatt gccgtagatg aaagactgag tgcgatatta 27300 tggtgtaata cataggaatt cgtttaaacg atctgcgtct aattttcggt ccaacttgca 27360 caggaaagac gtcgaccgcg gtagctcttg cccagcagac tgggcttcca gtcctttcgc 27420 tcgatcgggt ccaatgttgt cctcagctgt gaaccggaag cggacgacca acagtggaag 27480 aactgaaagg aacgagccgt ctataccttg atgatcggcc tctggtgaag ggtatcatcg 27540 cagccaagca agctcatgaa aggctgatgg gggaggtgta taattatgag gcccacggcg 27600 ggcttattct ttagggagga tctatctcgt tgctcaagtg catggcgcaa agcagttatt 27660 ggagtgcgga ttttcgttgg catattattc gccacgagtt agcagacgaa gagaccttca 27720 tgaacgtggc caaggccaga gttaagcaga tgttacgccc tgctgcaggc ctttctatta 27780 tccaatagtt ggttgatctt tggaaagagc ctcggctgag gcccatactg aaagagatcg 27840 atggatatcg atatgccatg ttgtttgcta gccagaacca gatcacatcc gatatgctat 27900 tgcagcttga cgcagatatg gaggataagt tgattcatgg gatcgctcag gagtagctca 27960 tccatgcacg ccgacaagaa cagaaattcc gtcgagttaa cgcagccgct tacgacggat 28020 tcgaaggtca tccattcgga atgtattagt ttgcaccagc tccgcgtcac acctgtcttc 28080 atttgaataa gatgttagca attgttttta gctttgtctt gttgtggcag ggcggcaagt 28140 gcttcagaca tcattctgtt ttcaaatttt atgctggaga acagcttctt aattcctttg 28200 gaaataatag actgcgtctt aaaattcaga tgtctggata tagatatgat tgtaaaataa 28260 cctatttaag tgtcatttag aacataagtt ttatgaatgt tcttccattt tcgtcatcga 28320 acgaataaga gtaaatacac cttttttaac attacaaata agttcttata cgttgtttat 28380 acaccgggaa tcatttccat tattttcgcg caaaagtcac ggatattcgt gaaagcgaca 28440 taaactgcga aatttgcggg gagtgtcttg agtttgcctc gaggctagcg catgcacata 28500 gacacacaca tcatctcatt gatgcttggt aataattgtc attagattgt ttttatgcat 28560 agatgcactc gaaatcagcc aattttagac aagtatcaaa cggatgtgac ttcagtacat 28620 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgattt acaattgaat 28680 atatcctgcc ccagccagcc aacagctcga tttacaattg aatatatcct gccggccggc 28740 ccacgcgtgt cgaggaattc tgatctggcc cccatttgga cgtgaatgta gacacgtcga 28800 aataaagatt tccgaattag aataatttgt ttattgcttt cgcctataaa tacgacggat 28860 cgtaatttgt cgttttatca aaatgtactt tcattttata ataacgctgc ggacatctac 28920 atttttgaat tgaaaaaaaa ttggtaatta ctctttcttt ttctccatat tgaccatcat 28980 actcattgct gatccatgta gatttcccgg acatgaagcc atttacaatt gaatatatcc 29040 tgccgccgct gccgctttgc acccggtgga gcttgcatgt tggtttctac gcagaactga 29100 gccggttagg cagataattt ccattgagaa ctgagccatg tgcaccttcc ccccaacacg 29160 gtgagcgacg gggcaacgga gtgatccaca tgggactttt aaacatcatc cgtcggatgg 29220 cgttgcgaga gaagcagtcg atccgtgaga tcagccgacg caccgggcag gcgcgcaaca 29280 cgatcgcaaa gtatttgaac gcaggtacaa tcgagccgac gttcacgcgg aacgaccaag 29340 caagcttggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg 29400 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc 29460 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt 29520 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg 29580 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat 29640 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg 29700 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg 29760 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc 29820 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa 29880 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa 29940 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg 30000 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaagggt 30060 cgacggatct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 30120 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 30180 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 30240 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 30300 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc 30360 aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga 30420 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc 30480 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc 30540 atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg 30600 cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag 30660 caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt 30720 ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc 30780 catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc ggaataccaa 30840 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta 30900 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg 30960 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag 31020 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac 31080 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac 31140 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc 31200 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac 31260 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc 31320 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa 31380 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc 31440 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc 31500 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt 31560 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg 31620 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg 31680 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac 31740 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg 31800 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga 31860 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa 31920 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc 31980 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg 32040 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc 32100 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc 32160 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg 32220 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac 32280 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac 32340 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg 32400 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat 32460 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag 32520 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa 32580 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc 32640 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc 32700 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc 32760 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct 32820 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc 32880 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc 32940 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc 33000 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca 33060 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg 33120 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg 33180 cgactcgaac ctacagttgt ttccttactg ggctttctca gcccccgagc gcttagtggg 33240 aatttgtacc ccttatcgaa ccgggagcac aggatgacgc ctaacaattc attcaagccg 33300 acaccgcttc gcggcgcggc ttaattcagg agttaaacat catgagggaa gcggtgatcg 33360 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 33420 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg 33480 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 33540 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 33600 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 33660 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 33720 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 33780 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 33840 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 33900 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 33960 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 34020 ctaggcaggc ttatcttgga caagaagatc gcttggcctc gcgcgcagat cagttggaag 34080 aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa 34140 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga gagctgggga 34200 agactatgcg cgatctgttg aaggtggttc taagcctcgt cttgcgatgg catttcgatc 34260 cattcccatt ccgcgctcaa gatggcttcc cctcggcagt tcatcagggc taaatcaatc 34320 tagccgactt gtccggtgaa atgggctgca ctccaacaga aacaatcaaa caaacataca 34380 cagcgactta ttcacacgag ctca 34404 <210> 48 <211> 38762 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7379 <400> 48 cttgtacaaa gtggttgcgg ccgcttaatt aaatttaaat tcaattaatg caatcttgat 60 tttcaacaac gaaggtaatg gcgtaaaaga aaaaatgtat gttattgtat tgatctttca 120 tgatgttgaa gcgtgccata atatgatgat gtataattaa aatattaact gtcgcatttt 180 attgaaatgg cactgttatt tcaaccatat ctttgattct gttacatgac acgactgcaa 240 gaagtaaata atagacgccg ttgttaaaga attgctatca tatgtgccta actagaggga 300 atttgagcgt cagacctaat caaatattac aaaatatctc actctgtcgc cagcaatggt 360 gtaatcagcg cagacaaatg gcgtaaagat cgcggaaaaa cctccccgag tggcatgata 420 gctgcctctg tattgctgat ttagtcagcc ttatttgact taagggtgcc ctcgttagtg 480 acaaattgct ttcaaggaga cagccatgcc ccacactttg ttgaaaaaca aattgccttt 540 ggggagacgg taaagccagt tgctcttcaa taaggaatgt cgaggaggca atgtaaccgc 600 ctctggtagt acacttctct aatccaaaaa tcaatttgta ttcaagatac cgcaaaaaac 660 ttatggttta aaccctgcag gactagtcca gaaggtaatt atccaagatg tagcatcaag 720 aatccaatgt ttacgggaaa aactatggaa gtattatgta agctcagcaa gaagcagatc 780 aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat acaagatcct 840 atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc aggcgaagaa 900 aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat aaggtcggtg 960 attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg gaaagtaacc 1020 ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc gtgtcatttt 1080 tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac aagaaaaaat 1140 ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa tttgtaagtt 1200 tgtaggtacc agatctggat cccaaaccat gtctccggag aggagaccag ttgagattag 1260 gccagctaca gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac 1320 gtctacagtg aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga 1380 gaggttgcaa gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat 1440 tgcttacgct gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt 1500 ttacgtgtca cataggcatc aaaggttggg cctaggatct acattgtaca cacatttgct 1560 taagtctatg gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga 1620 tccatctgtt aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc 1680 tggatacaag catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc 1740 agctcctcca aggccagtta ggccagttac ccaaatctga gtagttagct taatcaccta 1800 gagctcgatc ggcggcaata gcttcttagc gccatcccgg gttgatccta tctgtgttga 1860 aatagttgcg gtgggcaagg ctctctttca gaaagacagg cggccaaagg aacccaaggt 1920 gaggtgggct atggctctca gttccttgtg gaagcgcttg gtctaaggtg cagaggtgtt 1980 agcgggatga agcaaaagtg tccgattgta acaagatatg ttgatcctac gtaaggatat 2040 taaagtatgt attcatcact aatataatca gtgtattcca atatgtacta cgatttccaa 2100 tgtctttatt gtcgccgtat gtaatcggcg tcacaaaata atccccggtg actttctttt 2160 aatccaggat gaaataatat gttattataa tttttgcgat ttggtccgtt ataggaattg 2220 aagtgtgctt gaggtcggtc gccaccactc ccatttcata attttacatg tatttgaaaa 2280 ataaaaattt atggtattca atttaaacac gtatacttgt aaagaatgat atcttgaaag 2340 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa 2400 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc 2460 tggtacattg ccgtagatga aagactgagt gcgatattat ggtgtaatac ataggaattc 2520 gtttaaacga tctgcgtcta attttcggtc caacttgcac aggaaagacg tcgaccgcgg 2580 tagctcttgc ccagcagact gggcttccag tcctttcgct cgatcgggtc caatgttgtc 2640 ctcagctgtg aaccggaagc ggacgaccaa cagtggaaga actgaaagga acgagccgtc 2700 tataccttga tgatcggcct ctggtgaagg gtatcatcgc agccaagcaa gctcatgaaa 2760 ggctgatggg ggaggtgtat aattatgagg cccacggcgg gcttattctt tagggaggat 2820 ctatctcgtt gctcaagtgc atggcgcaaa gcagttattg gagtgcggat tttcgttggc 2880 atattattcg ccacgagtta gcagacgaag agaccttcat gaacgtggcc aaggccagag 2940 ttaagcagat gttacgccct gctgcaggcc tttctattat ccaatagttg gttgatcttt 3000 ggaaagagcc tcggctgagg cccatactga aagagatcga tggatatcga tatgccatgt 3060 tgtttgctag ccagaaccag atcacatccg atatgctatt gcagcttgac gcagatatgg 3120 aggataagtt gattcatggg atcgctcagg agtagctcat ccatgcacgc cgacaagaac 3180 agaaattccg tcgagttaac gcagccgctt acgacggatt cgaaggtcat ccattcggaa 3240 tgtattagtt tgcaccagct ccgcgtcaca cctgtcttca tttgaataag atgttagcaa 3300 ttgtttttag ctttgtcttg ttgtggcagg gcggcaagtg cttcagacat cattctgttt 3360 tcaaatttta tgctggagaa cagcttctta attcctttgg aaataataga ctgcgtctta 3420 aaattcagat gtctggatat agatatgatt gtaaaataac ctatttaagt gtcatttaga 3480 acataagttt tatgaatgtt cttccatttt cgtcatcgaa cgaataagag taaatacacc 3540 ttttttaaca ttacaaataa gttcttatac gttgtttata caccgggaat catttccatt 3600 attttcgcgc aaaagtcacg gatattcgtg aaagcgacat aaactgcgaa atttgcgggg 3660 agtgtcttga gtttgcctcg aggctagcgc atgcacatag acacacacat catctcattg 3720 atgcttggta ataattgtca ttagattgtt tttatgcata gatgcactcg aaatcagcca 3780 attttagaca agtatcaaac ggatgtgact tcagtacatt aaaaacgtcc gcaatgtgtt 3840 attaagttgt ctaagcgtca atttgattta caattgaata tatcctgccc cagccagcca 3900 acagctcgat ttacaattga atatatcctg ccggccggcc cacgcgtgtc gaggaattct 3960 gatctggccc ccatttggac gtgaatgtag acacgtcgaa ataaagattt ccgaattaga 4020 ataatttgtt tattgctttc gcctataaat acgacggatc gtaatttgtc gttttatcaa 4080 aatgtacttt cattttataa taacgctgcg gacatctaca tttttgaatt gaaaaaaaat 4140 tggtaattac tctttctttt tctccatatt gaccatcata ctcattgctg atccatgtag 4200 atttcccgga catgaagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4260 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4320 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4380 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4440 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 4500 caggtacaat cgagccgacg ttcacgcgga acgaccaagc aagcttggct gccatttttg 4560 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 4620 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 4680 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 4740 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 4800 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 4860 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 4920 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 4980 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 5040 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 5100 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 5160 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 5220 gcggcgaggg caaccagccc ggtgagcgtc ggaaagggtc gacggatctt ttccgctgca 5280 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 5340 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 5400 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 5460 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc 5520 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc 5580 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg 5640 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg 5700 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc 5760 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat 5820 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc 5880 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg 5940 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc 6000 ggagctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 6060 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 6120 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 6180 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 6240 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 6300 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 6360 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 6420 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 6480 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 6540 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 6600 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 6660 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 6720 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 6780 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 6840 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 6900 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 6960 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 7020 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 7080 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 7140 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 7200 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 7260 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 7320 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 7380 tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 7440 ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 7500 gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc 7560 cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag 7620 aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac 7680 atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct 7740 cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc 7800 ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt 7860 ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc 7920 tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc 7980 gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga 8040 ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca 8100 ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg 8160 gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc 8220 gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg 8280 ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga 8340 gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt 8400 tccttactgg gctttctcag cccccgagcg cttagtggga atttgtaccc cttatcgaac 8460 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct 8520 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 8580 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 8640 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 8700 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 8760 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 8820 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 8880 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 8940 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 9000 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 9060 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 9120 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 9180 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac 9240 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag 9300 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 9360 tcgcggcgcg gcttaactca agcgttagag agctggggaa gactatgcgc gatctgttga 9420 aggtggttct aagcctcgtc ttgcgatggc atttcgatcc attcccattc cgcgctcaag 9480 atggcttccc ctcggcagtt catcagggct aaatcaatct agccgacttg tccggtgaaa 9540 tgggctgcac tccaacagaa acaatcaaac aaacatacac agcgacttat tcacacgagc 9600 tcaaattaca acggtatata tcctgccagt cagcatcatc acaccaaaag ttaggcccga 9660 atagtttgaa attagaaagc tcgcaattga ggtctacagg ccaaattcgc tcttagccgt 9720 acaatattac tcaccggatc ctaaccggtt taattaaggc gcgccatgcc cgggcaagcg 9780 gccgcacaag tttgtacaaa aaagcaggct tctagaccta ggtggagtca tcacgcagac 9840 tatctcagca tgtgcgtagc acgcggccgc ctcccagtat cattatagtg aaagttttgg 9900 ctctctcgcc ggtggttttt tacctctatt taaaggggtt ttccacctaa aaattctggt 9960 atcattctca ctttacttgt tactttaatt tctcataatc tttggttgaa attatcacgc 10020 ttccgcacac gatatcccta caaatttatt atttgttaaa cattttcaaa ccgcataaaa 10080 ttttatgaag tcccgtctat ctttaatgta gtctaacatt ttcatattga aatatataat 10140 ttacttaatt ttagcgttgg tagaaagcat aatgatttat tcttattctt cttcatataa 10200 atgtttaata tacaatataa acaaattctt taccttaaga aggatttccc attttatatt 10260 ttaaaaatat atttatcaaa tatttttcaa ccacgtaaat ctcataataa taagttgttt 10320 caaaagtaat aaaatttaac tccataattt ttttattcga ctgatcttaa agcaacaccc 10380 agtgacacaa ctagccattt ttttctttga ataaaaaaat ccaattatca ttgtattttt 10440 tttatacaat gaaaatttca ccaaacaatg atttgtggta tttctgaagc aagtcatgtt 10500 atgcaaaatt ctataattcc catttgacac tacggaagta actgaagatc tgcttttaca 10560 tgcgagacac atcttctaaa gtaattttaa taatagttac tatattcaag atttcatata 10620 tcaaatactc aatattactt ctaaaaaatt aattagatat aattaaaata ttactttttt 10680 aattttaagt ttaattgttg aatttgtgac tattgattta ttattctact atgtttaaat 10740 tgttttatag atagtttaaa gtaaatataa gtaatgtagt agagtgttag agtgttaccc 10800 taaaccataa actataagat ttatggtgga ctaattttca tatatttctt attgctttta 10860 ccttttcttg gtatgtaagt ccgtaactgg aattactgtg ggttgccatg acactctgtg 10920 gtcttttggt tcatgcatgg atcttgcgca agaaaaagac aaagaacaaa gaaaaaagac 10980 aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa ttagtcactg gctgatcaag 11040 atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa cacgtgctta acatgcactt 11100 taaatggctc acccatctca acccacacac aaacacattg cctttttctt catcatcacc 11160 acaaccacct gtatatattc attctcttcc gccacctcaa tttcttcact tcaacacacg 11220 tcaacctgca tatgcgtgtc atcccatgcc caaatctcca tgcatgttcc aaccaccttc 11280 tctcttatat aatacctata aatacctcta atatcactca cttctttcat catccatcca 11340 tccagagtac tactactcta ctactataat accccaaccc aactcatatt caatactact 11400 ctaggatcca caaccaaaaa tggcagcgag gctgcaagag cagaaaggag gtgagatgga 11460 cacgaggatt gcgatcatag gcatgagcgc catacttcct tgtggaacca cggtgagaga 11520 gtcatgggag accataaggg ctggcattga ttgcctgtct gatcttcctg aggatcgtgt 11580 tgatgtgact gcgtactttg acccagtcaa gaccacaaag gacaaaatct actgcaagag 11640 aggaggtttc attcctgagt atgactttga tgcgcgtgag tttggtctca acatgttcca 11700 gatggaagat tctgatgcga atcagaccat ctcacttctc aaggtcaaag aagcacttca 11760 agatgctggg attgatgcac ttggaaagga gaagaaaaac ataggatgcg tgcttggcat 11820 tggaggtgga cagaagagtt cacatgagtt ctacagcaga ctcaactatg tggttgtgga 11880 gaaagtcttg agaaagatgg ggatgccaga ggaagatgtc aaagtggctg tggagaagta 11940 caaagcgaac ttcccagaat ggagacttga ctctttccct ggattccttg ggaatgtcac 12000 tgctggcaga tgcaccaaca cattcaactt ggatggcatg aactgcgttg tggatgctgc 12060 atgtgcaagc tccttgatag cagtcaaggt tgcgattgat gaactgctct atggtgactg 12120 cgacatgatg gtcactggtg caacctgcac agacaactcc attgggatgt acatggcttt 12180 ctcaaagaca cctgtgttca gcacggaccc aagtgtgaga gcgtatgatg agaaaacgaa 12240 agggatgctc attggtgagg gatcagccat gttggtgctc aaacgttatg cagatgcagt 12300 gagggatggt gatgagattc atgctgtgat tcgtggttgt gcaagctcaa gtgatggcaa 12360 agcagctggc atctacactc ccacaatcag tggtcaagag gaagccttga gacgtgcata 12420 caatcgtgcc tgcgttgacc ctgccactgt cactttggtt gagggtcatg ggactggcac 12480 tccagttgga gataggattg agttgactgc gttgaggaac ttgtttgaca aagcgtatgg 12540 tgaagggaac acagagaaag ttgcagttgg aagtatcaag tccagtattg gtcatttgaa 12600 ggctgttgct ggtcttgctg gaatgatcaa ggtcatcatg gctctcaaac acaagacttt 12660 gcctggaacg atcaatgtgg acaatccacc caatctctat gacaacactc ccatcaatga 12720 gtcatccctc tacatcaaca cgatgaacag accctggttc cctccacctg gtgttccgag 12780 acgtgctgga atatccagct ttggctttgg aggtgccaac taccatgcag tgttggaaga 12840 ggctgaacca gagcacacga cagcgtacag actcaacaaa cgtccacaac cggtgttgat 12900 gatggctgca actcctgctg cgttgcagag cctttgtgaa gctcagctga aagagtttga 12960 agctgcgatc aaggagaatg agacagtcaa gaatactgcc tacatcaagt gtgtcaagtt 13020 tggagaacag ttcaagttcc ctgggagcat acctgcgacc aatgcgagac tgggattctt 13080 ggtcaaagat gcggaggatg cgtgctccac gcttcgtgct atatgtgctc agtttgccaa 13140 agatgtgacc aaagaagcgt ggaggcttcc gagagaagga gtttctttcc gtgccaaagg 13200 cattgccacc aatggtgctg ttgctgcact cttctctgga caaggtgctc agtacacaca 13260 catgttcagt gaagttgcga tgaactggcc tcagttcaga cagtccattg ctgcaatgga 13320 cgctgcacag tccaaggttg ctggaagtga caaagacttt gaaagagtct ctcaagtcct 13380 gtacccaagg aaaccgtacg aacgtgagcc agaacaagat cacaagaaaa tctcactcac 13440 agcatacagc cagccgtcaa ccctggcatg tgctcttggt gcctttgaga ttttcaagga 13500 agctggattc actccagact ttgcagctgg acacagcttg ggtgagtttg ctgcgctcta 13560 tgcagctggt tgtgttgaca gagatgagtt gtttgaactt gtttgcagaa gggcaaggat 13620 catgggaggc aaagatgcac ctgccacacc caaaggttgc atggcagctg tcataggacc 13680 caacgcagag aacatcaagg ttcaagctgc caacgtctgg cttggaaact caaactctcc 13740 gtctcagact gtcattactg ggtctgttga agggattcaa gctgaaagtg ccagattgca 13800 gaaggaaggg ttcagagttg tgcctcttgc ttgtgagtct gccttccatt ctccacagat 13860 ggaaaatgcc agctcagctt tcaaagatgt catatccaag gtttccttcc gtacacccaa 13920 agcagaaaca aagctcttca gcaatgtttc tggagagacc tatccgacgg atgccagaga 13980 gatgctcact caacacatga cctcatctgt gaagttcctg acccaagtga ggaacatgca 14040 ccaagctggt gcgagaatct ttgttgagtt tggaccgaaa caagttctca gcaaacttgt 14100 ttctgaaacc ctcaaagatg acccgtctgt tgtcacagtg tctgtgaacc cagcttctgg 14160 cacggactct gacatacagt tgagggatgc tgcagttcag ctggttgtgg ctggtgtcaa 14220 tcttcaaggg tttgacaagt gggatgctcc tgatgccacg aggatgcaag ccatcaagaa 14280 aaagagaacc acactcagac tttcagctgc cacctatgtc agtgacaaga ccaagaaagt 14340 gagggatgct gccatgaacg acggaagatg tgtcacatac ctcaagggtg ctgcaccact 14400 catcaaggct cctgaacctg tggttgacga agctgccaag agggaggctg aaaggcttca 14460 gaaggagctt caagacgctc agaggcagct tgacgacgcc aaacgtgcag ctgcagaagc 14520 caactccaaa cttgctgctg ccaaggagga agccaagact gctgcagctt cagccaagcc 14580 tgcagttgac actgctgttg tggagaaaca tcgtgccatc ctcaagtcca tgctggctga 14640 gctggatggt tatggaagtg ttgatgcttc ttcccttcag caacagcaac agcaacagac 14700 tgctccggcg cctgttaaag ccgctgcccc tgccgctccc gttgcaagtg ctcctgctcc 14760 cgccgtgtcc aacgagttgc ttgagaaagc tgagacagtt gtgatggagg ttctcgctgc 14820 caagactgga tacgaaacag acatgattga ggcggacatg gagcttgaga ctgagttggg 14880 aattgacagc atcaagaggg ttgaaattct ttctgaagtc caagctatgc ttaacgttga 14940 ggcgaaagat gttgatgctc tctctaggac gagaacagtg ggtgaagttg tcaacgctat 15000 gaaggcagaa atcgctggtt cttccgctcc ggcgccggca gctgccgcac ctgcaccggc 15060 taaagcggct ccagctgcag ctgcaccagc tgtttctaat gagctgctcg aaaaggcaga 15120 aactgttgtg atggaagtcc ttgctgccaa aactggctac gaaactgaca tgatagaatc 15180 agacatggag cttgagacgg agcttggaat tgattccatc aagcgtgtgg aaatcctcag 15240 tgaagtccaa gccatgctca atgtggaagc taaggatgtg gatgctctct caaggaccag 15300 aactgtcggt gaggttgtca atgccatgaa ggctgagata gctggaggct ccgctcctgc 15360 gccggccgct gccgctcctg gtcccgctgc cgcggctcca gctccagctg cagccgctcc 15420 ggccgtctca aacgaactcc ttgaaaaagc tgagaccgtc gtgatggagg tcttggcagc 15480 taaaaccggc tatgagactg acatgattga gtctgacatg gaattggaga cagaacttgg 15540 gatagacagc atcaaacgtg ttgaaatcct cagtgaggtc caagctatgc tgaacgtgga 15600 agcgaaggat gttgatgctc tgtccagaac gaggaccgtt ggtgaggttg tggatgccat 15660 gaaggctgaa attgctggag ggtctgcgcc cgcgccagct gcagccgctc ctgcacccgc 15720 agccgcggca cctgctccag ctgcacctgc acctgccgtt tcttcagaac tcttggagaa 15780 ggctgagacg gttgtgatgg aggttttggc tgcaaagact ggctatgaga ccgacatgat 15840 tgaatctgat atggaattgg agacagagct tgggatcgat tccatcaaaa gagtggagat 15900 cctgagtgaa gtccaagcca tgctcaatgt tgaagctaag gatgttgatg cactttcaag 15960 aacaaggaca gtgggtgagg ttgtggatgc catgaaggcg gagattgctg gagggagcgc 16020 gcctgcgcca gctgccgcgg cacctgcacc ggcagccgca gctccagctc cagctgcacc 16080 agcaccggcc gcgcccgcgc cagctgtttc atccgaactc ctggaaaagg ccgagacggt 16140 tgtgatggag gtgctcgcag ctaagactgg ttacgagacc gacatgattg aaagtgacat 16200 ggagcttgaa accgagttgg gaatagatag catcaaacgt gttgaaatct tgtctgaggt 16260 ccaagctatg ttgaacgtgg aggcaaaaga tgtcgatgcg ctttcaagaa ccagaacagt 16320 cggtgaggtc gtggacgcca tgaaggctga aattgccgga agctctgcta gtgcgccagc 16380 tgcagccgca cctgcacctg ctgccgcggc acctgctcca gctgcagccg ctcctgccgt 16440 cagcaatgag ttgctggaga aagccgaaac agttgtgatg gaagtccttg ctgcaaaaac 16500 tggttacgaa acggacatga ttgagtcaga catggaattg gagaccgaac ttggaataga 16560 ttccatcaag agagtggaga tcctctctga ggtgcaagcc atgctcaacg ttgaggctaa 16620 agatgttgat gcactcagta ggacaagaac tgtgggggaa gttgtcgatg cgatgaaggc 16680 tgagattgct ggaggctctg ctcctgcgcc ggcagctgcc gcacctgcac ccgcagccgc 16740 ggcaccagct gtcagcaacg aattgcttga gaaggctgaa acagttgtca tggaagtgct 16800 ggctgcaaaa accggctatg agactgacat gattgagtct gacatggaac ttgagacgga 16860 attgggcatt gacagcatca agcgtgttga gatcctcagt gaagtccaag caatgctcaa 16920 tgtggaggct aaggatgtgg atgccctctc aaggaccaga acagttggtg aggtcgttga 16980 tgcgatgaag gcggagatcg ctggaagttc cgcgcctgcg ccagctgcag ccgctcctgc 17040 acccgcagcc gcggcaccgg ctccagctgc agccgcaccg gccgtctcct cagaactcct 17100 ggaaaaggct gagacagttg tgatggaagt ccttgctgcg aaaactggct atgagaccga 17160 catgattgaa tctgacatgg aactggagac cgagcttgga attgactcca tcaaacgtgt 17220 tgaaatcctc tctgaggttc aagcgatgtt gaatgtggag gccaaagatg ttgatgctct 17280 ttccagaaca aggacggtgg gagaggtggt tgatgccatg aaggctgaga tagctggggg 17340 ttctgctcct gcgccagctg cagccgctcc tgctcccgct gccgcggcac cagctgtcag 17400 caacgaactc cttgaaaaag ctgaaactgt tgtgatggag gttttggctg caaagactgg 17460 atacgagact gacatgatag agtcagacat ggagttggaa acagagcttg ggattgatag 17520 catcaagcgt gtggaaatcc tttctgaggt tcaagccatg ctgaacgttg aggccaaaga 17580 tgtcgatgct ttgtcaagga ccagaacggt tggagaagtg gtcgatgcca tgaaggctga 17640 gatagctggt ggatcagccc ctgctccggc tgcagccgct cctgcaagtg ccggtgctgc 17700 accagctgtg aagattgatt cagttcatgg tgctgactgt gacgatttgt cactgatgca 17760 cgcaaaggtg gttgacatca gaaggccaga tgaactgatt ctggaaagac cggagaaccg 17820 tccagttctt gtcgttgacg atggctcaga gttgacactt gctcttgtga gggtgttggg 17880 agcctgtgca gttgtcttga cctttgaggg attgcagttg gctcagagag ctggtgcagc 17940 tgccattcgt catgttctgg ctaaggacct ttcagcagaa tcagctgaga aggccatcaa 18000 agaggctgag caaagatttg gagcgttggg aggcttcata agccagcaag cagaaaggtt 18060 tgaaccagct gagatactgg gtttcacact catgtgtgcg aagtttgcca aagcctctct 18120 ctgcactgct gtggctggag gtcgtcctgc cttcatcggt gtggctaggc ttgacggcag 18180 acttggattc acctctcaag gcacttctga cgcactcaag agagcacagc gtggagcgat 18240 ctttggactc tgcaagacaa tagggttgga gtggtcagaa tctgatgtgt tcagccgtgg 18300 tgtggacatt gctcaaggga tgcacccaga agatgctgca gttgccatag ttagggagat 18360 ggcgtgtgct gacatcagaa tcagagaggt tgggattgga gcaaaccagc aaagatgcac 18420 aatcagagct gccaagttgg agactgggaa ccctcagaga cagatagcga aagacgatgt 18480 gcttctggtt tctggaggtg ctcgtgggat cacacctctt tgcatccgtg agatcacgcg 18540 tcagattgct ggaggcaagt acattctgtt gggtcgtagc aaagtttctg cgtctgaacc 18600 agcatggtgt gctggcatca ctgatgagaa ggctgtgcag aaagctgcca cccaagagtt 18660 gaagagggcc ttcagtgctg gtgaaggacc caaacccact ccaagggctg tcacgaaact 18720 tgtgggatct gttcttggtg ctcgtgaagt gagaagctcc attgctgcca ttgaagctct 18780 gggtggcaaa gcgatctaca gctcttgtga tgtgaactct gctgcagatg ttgccaaagc 18840 agttagggat gcagagtccc agcttggtgc cagagttagt ggaatcgttc acgcttctgg 18900 tgtgctccgt gatcgtctca tagagaagaa actccctgac gagtttgatg ctgtctttgg 18960 gaccaaagtc actgggttgg agaatcttct ggctgcagtt gatcgtgcca acctcaaaca 19020 catggttctc ttcagctccc ttgctggttt ccacggcaat gttggtcagt ctgactatgc 19080 gatggcaaac gaagctctca acaagatggg tttggaactt gcgaaggatg tcagtgtcaa 19140 gtccatctgc tttggtccct gggatggtgg gatggtgaca cctcaactca agaaacagtt 19200 tcaagaaatg ggagttcaga tcattcctcg tgaaggaggt gctgatacag ttgcgaggat 19260 tgtgttggga tcaagtcctg ctgagatcct tgttggaaac tggaggacac catccaagaa 19320 agttggaagt gacacgatca ccctgcatag aaagatcagt gccaagtcca atcccttctt 19380 ggaggatcat gtcatacaag gcagaagggt gttgccaatg acccttgcta ttgggagcct 19440 tgctgagacc tgccttggac tcttccctgg ctactctctc tgggccatag atgacgcaca 19500 gctcttcaaa ggtgtcactg ttgatggaga cgtcaactgt gaagtgaccc tcacaccgtc 19560 cactgctccg agtgggaggg tgaatgtcca agcgacgctc aagacgttct caagtggaaa 19620 acttgttcca gcctacagag ctgtcatagt gttgtccaac caaggtgctc cacctgccaa 19680 tgcaaccatg cagcctccga gccttgatgc agatcctgct ttgcaagggt cagtctatga 19740 tggcaagact ctctttcatg gtcctgcgtt cagaggcatt gatgacgttc tcagctgcac 19800 caagagccag cttgttgcca agtgttctgc tgttcctgga tcagatgcag ccagaggaga 19860 gtttgcaact gacactgatg cacatgatcc ctttgtcaat gatcttgcct ttcaagcaat 19920 gttggtctgg gtgaggagaa cgctgggaca agctgcactc ccgaactcca ttcagaggat 19980 agtccaacac agaccggtcc cacaagacaa acccttctac atcaccctca gatccaatca 20040 gagtggtgga catagccagc acaaacatgc acttcagttc cacaacgaac aaggtgatct 20100 cttcattgat gttcaagcct ctgtcattgc gacagactca cttgcgttct gagtagttag 20160 cttaatcact taggtcacct cgagtatcaa aatctattta gaaatacaca atattttgtt 20220 gcaggcttgc tggagaatcg atctgctatc ataaaaatta caaaaaaatt ttatttgcct 20280 caattatttt aggattggta ttaaggacgc ttaaattatt tgtcgggtca ctacgcatca 20340 ttgtgattga gaagatcagc gatacgaaat attcgtagta ctatcgataa tttatttgaa 20400 aattcataag aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga cagataaagc 20460 cacgcacatt taggatattg gccgagatta ctgaatattg agtaagatca cggaatttct 20520 gacaggagca tgtcttcaat tcagcccaaa tggcagttga aatactcaaa ccgccccata 20580 tgcaggagcg gatcattcat tgtttgtttg gttgcctttg ccaacatggg agtccaaggt 20640 tggcgcgccg aattcgcggc cgcttaatta agatgagtga tactcaggac tcaggactca 20700 ctctgctgat cactagtgct agcctcgagg tcgacacaac tttgtataca aaagttgtct 20760 agacctaggc tcagcaactc atccagtcaa gttcgagtca gtgagttggg cggccgcctc 20820 ccagtatcat tatagtgaaa gttttggctc tctcgccggt ggttttttac ctctatttaa 20880 aggggttttc cacctaaaaa ttctggtatc attctcactt tacttgttac tttaatttct 20940 cataatcttt ggttgaaatt atcacgcttc cgcacacgat atccctacaa atttattatt 21000 tgttaaacat tttcaaaccg cataaaattt tatgaagtcc cgtctatctt taatgtagtc 21060 taacattttc atattgaaat atataattta cttaatttta gcgttggtag aaagcataat 21120 gatttattct tattcttctt catataaatg tttaatatac aatataaaca aattctttac 21180 cttaagaagg atttcccatt ttatatttta aaaatatatt tatcaaatat ttttcaacca 21240 cgtaaatctc ataataataa gttgtttcaa aagtaataaa atttaactcc ataatttttt 21300 tattcgactg atcttaaagc aacacccagt gacacaacta gccatttttt tctttgaata 21360 aaaaaatcca attatcattg tatttttttt atacaatgaa aatttcacca aacaatgatt 21420 tgtggtattt ctgaagcaag tcatgttatg caaaattcta taattcccat ttgacactac 21480 ggaagtaact gaagatctgc ttttacatgc gagacacatc ttctaaagta attttaataa 21540 tagttactat attcaagatt tcatatatca aatactcaat attacttcta aaaaattaat 21600 tagatataat taaaatatta cttttttaat tttaagttta attgttgaat ttgtgactat 21660 tgatttatta ttctactatg tttaaattgt tttatagata gtttaaagta aatataagta 21720 atgtagtaga gtgttagagt gttaccctaa accataaact ataagattta tggtggacta 21780 attttcatat atttcttatt gcttttacct tttcttggta tgtaagtccg taactggaat 21840 tactgtgggt tgccatgaca ctctgtggtc ttttggttca tgcatggatc ttgcgcaaga 21900 aaaagacaaa gaacaaagaa aaaagacaaa acagagagac aaaacgcaat cacacaacca 21960 actcaaatta gtcactggct gatcaagatc gccgcgtcca tgtatgtcta aatgccatgc 22020 aaagcaacac gtgcttaaca tgcactttaa atggctcacc catctcaacc cacacacaaa 22080 cacattgcct ttttcttcat catcaccaca accacctgta tatattcatt ctcttccgcc 22140 acctcaattt cttcacttca acacacgtca acctgcatat gcgtgtcatc ccatgcccaa 22200 atctccatgc atgttccaac caccttctct cttatataat acctataaat acctctaata 22260 tcactcactt ctttcatcat ccatccatcc agagtactac tactctacta ctataatacc 22320 ccaacccaac tcatattcaa tactactcta ggatccacaa ccaaaaatgg ctgcaaggaa 22380 cgtgagtgcg gctcatgaga tgcatgacga gaagaggata gctgttgtgg gcatggctgt 22440 tcagtatgct ggatgcaaaa cgaaagacga gttctgggaa gttctcatga atggaaaagt 22500 tgagagcaaa gtgatcagcg acaaacgtct tggttcaaat taccgtgctg aacactacaa 22560 agctgaaagg agcaagtatg ctgacacctt ctgcaatgag acatatggaa ctcttgatga 22620 gaacgaaata gacaatgagc atgaactcct tttgaacctt gccaaacaag ccttggctga 22680 aacatctgtc aaagattcaa caagatgtgg catagtttct ggatgcctca gcttcccgat 22740 ggacaacctc caaggtgaac tcttgaatgt gtaccagaac catgttgaga agaaacttgg 22800 tgccagagtt ttcaaagatg cctctcactg gtcagaaaga gaacagtcca acaaaccaga 22860 ggctggtgac agaagaatct tcatggaccc tgcttccttt gttgctgagg aactcaatct 22920 tggcgctctc cactactctg ttgatgctgc ctgtgccact gcgctctatg tgctcagact 22980 ggcgcaagat cacttggtct ctggtgcagc tgatgtgatg ctttgtggtg caacatgcct 23040 gccggagccg tttttcatac tctctgggtt ctcaaccttc caagccatgc ctgttggaac 23100 gggtcagaat gtgtccatgc cgctgcacaa agattctcaa ggattgaccc ctggtgaagg 23160 aggttccatc atggtcctca aacgtttgga tgacgcaatc agagatggag atcacatcta 23220 tggaacactt ttgggagcca atgtcagcaa cagtggcact ggtctgccac ttaaacccct 23280 tttgccgtca gagaaaaagt gcctcatgga cacctacaca cgtatcaatg tgcatcccca 23340 caagattcag tatgttgaat gccatgcaac tgggactcca caaggtgatc gtgtggaaat 23400 tgatgctgtg aaggcttgct ttgaaggcaa agtcccacgt tttggaacca caaaagggaa 23460 ctttggacac acattggtgg cagctgggtt tgctggcatg tgcaaagtgc ttctctccat 23520 gaagcacggg atcattccac ccactccggg aatagatgac gaaacgaaga tggaccctct 23580 ggttgtctct ggtgaggcca ttccctggcc agaaacgaat ggagaaccga agcgtgctgg 23640 tctctcagcg tttggatttg gtgggaccaa tgctcatgct gtctttgagg aacatgaccc 23700 aagcaatgct gcatgcactg gccatgactc catttctgct ctctctgcac gttgtggtgg 23760 tgagtcaaac atgagaatag caatcactgg gatggatgcc acttttggtg ctttgaaagg 23820 attggatgct tttgaaaggg ccatctacac tggtgctcat ggtgctatac cactcccaga 23880 aaagagatgg agattcctgg ggaaagacaa ggacttcctt gatctttgtg gagtcaaagc 23940 aacaccgcat ggctgttaca ttgaagatgt tgaagttgat ttccagaggc tgaggactcc 24000 aatgactcct gaagatatgc tcttgcctca acagcttctg gctgtcacca caattgatag 24060 ggccatattg gactctggca tgaagaaagg tggaaatgtg gctgtctttg tggggttggg 24120 gaccgatctt gaactctacc gtcaccgtgc aagggttgct ctcaaggaga gagtgaggcc 24180 tgaagccagc aagaaactca atgacatgat gcagtacatc aatgattgtg ggacaagcac 24240 ttcttacacc agctacatag gcaacttggt tgcaacaagg gtcagttcac aatggggctt 24300 cacgggacct tccttcacaa tcacagaagg caacaattct gtgtacagat gtgctgaact 24360 tggcaaatac ctcttggaaa cgggagaggt tgatggggtt gtggttgcgg gtgttgatct 24420 ctgtgggagt gctgagaacc tttacgtcaa gtcaaggcgt ttcaaggtgt ctacatctga 24480 caccccgaga gccagctttg atgcagctgc tgatggttac tttgttggag agggctgtgg 24540 tgcatttgtg ctcaagcgtg agacaagctg caccaaggat gacagaatct atgcttgcat 24600 ggatgccata gttcctggaa atgttccatc tgcatgtttg agagaggcat tggatcaagc 24660 cagagtcaag cctggagaca tcgagatgtt ggagctttct gctgattcag cgaggcattt 24720 gaaggacccg tctgttctcc caaaggaact cactgctgag gaagagattg gcggacttca 24780 gacgattctt cgtgacgatg acaagctccc aaggaatgtt gcaactggaa gtgtcaaggc 24840 cactgttggt gacactggtt atgcttctgg tgctgccagt ttgattaagg ctgcgctttg 24900 catctacaac cgttacctgc ccagcaatgg agatgactgg gatgaacctg cacctgaagc 24960 accctgggac agcacactct ttgcgtgcca gacctcaaga gcttggttga agaaccctgg 25020 tgagagacgt tatgcggctg tttccggagt cagtgaaacc agaagttgct acagtgtgct 25080 gctttctgaa gcagagggac actacgagag agagaacaga atcagtttgg atgaggaagc 25140 acccaagctc attgtgttga gagcagactc ccatgaagag attcttggaa gattggacaa 25200 gataagggaa aggttcttgc agcccacggg tgctgcaccc agagagtctg aattgaaggc 25260 gcaagcccgt aggatctttc ttgaactcct tggtgagacc cttgcccaag atgcagcttc 25320 atctggaagt cagaagcccc ttgctctcag cttggtctcc actccatcca agctccagag 25380 agaggtggaa cttgcggcaa aaggcattcc gagatgtctc aagatgagac gtgattggag 25440 ttcccctgct ggaagcagat atgcgcctga gcctttggcc agtgacagag ttgccttcat 25500 gtatggagaa ggaaggagcc cttactatgg catcacacaa gacattcata ggatttggcc 25560 tgaacttcat gaggtcatca atgagaaaac aaaccgtctc tgggctgaag gtgacagatg 25620 ggtcatgcca agggccagct tcaagagtga gcttgagtca caacagcaag agtttgacag 25680 aaacatgatt gaaatgttcc gtcttggaat cctcacctca attgccttca caaacctggc 25740 gagagatgtt ctcaacatca cgcccaaagc agcctttgga ctcagtttgg gagagatttc 25800 catgatcttt gctttctcca agaaaaacgg tttgataagc gatcagttga caaaggattt 25860 gagagagtca gatgtctgga acaaagctct ggctgttgag ttcaatgcgc tcagagaagc 25920 ctggggcatt ccacagtctg tcccaaaaga tgagttctgg caaggttaca tagtgagggg 25980 aacgaaacaa gacatagaag cagccatagc accagactca aagtatgtga ggttgaccat 26040 catcaatgat gccaacactg ctctcatcag tggaaagcct gatgcatgca aagctgcgat 26100 tgctcgtctt ggtggcaaca tccctgcgct tccagtcact caaggcatgt gtgggcattg 26160 cccagaggtg ggaccttaca ccaaggacat cgcaaagatc cacgccaacc ttgagttccc 26220 tgtggttgat ggtcttgatc tctggactac cataaaccag aagaggcttg tgccaagagc 26280 cacgggtgcc aaagatgaat gggctccttc atcctttggg gagtatgctg gacagctcta 26340 tgagaaacaa gccaacttcc ctcagatagt tgaaaccatc tacaagcaga actatgatgt 26400 gtttgttgaa gttgggccaa acaatcacag aagcacagca gtgagaacca cgcttggacc 26460 acagagaaac caccttgctg gtgccataga caagcagaac gaggatgctt ggacgacaat 26520 agtcaagctc gtggcttcat tgaaggctca ccttgttcct ggagtgacca tcagtccgct 26580 ctaccactcc aagctcgttg cagaggctga ggcttgttat gctgcgctct gtaaaggtga 26640 gaaacccaag aaaaacaagt ttgtgagaaa gattcagttg aatggcagat tcaacagcaa 26700 ggcagacccc atctcctcag cagaccttgc atccttccca cctgcggacc ctgcgattga 26760 agctgccatc tcttcaagga tcatgaagcc tgtggctccg aaattctatg cgcgtctcaa 26820 cattgatgag caagatgaga ccagagatcc gatcttgaac aaggacaatg cgccttccag 26880 ctccagctct tccagcagtt cctcttccag cagttcatct ccgtctcctg ctccttcagc 26940 tccagttcag aagaaagctg ccccagctgc ggagacaaaa gctgttgcca gtgctgatgc 27000 acttcgtagt gccctgcttg atcttgactc catgcttgcg ctcagctctg caagtgcttc 27060 tggcaacctt gttgagactg cgccatctga tgccagtgtc attgtccctc cgtgcaacat 27120 tgctgatctt ggcagcagag ctttcatgaa aacttatggt gtttctgcgc ctctgtacac 27180 cggtgcaatg gccaagggaa ttgcctctgc ggacttggtg atcgcagctg gaaggcaagg 27240 aatccttgcg tcatttggtg ctggaggcct tccaatgcaa gttgtgcgtg agtccattga 27300 aaagattcaa gctgccctgc ccaatggacc atatgctgtc aacctcatcc attctccttt 27360 tgacagcaac cttgagaagg gaaatgttga tctcttcttg gaaaagggtg tcacttttgt 27420 tgaggcttca gccttcatga cgttgacacc gcaagttgtc agatacagag cggctgggtt 27480 gacgaggaat gcagatgggt cagtcaacat aaggaacaga atcattggga aagtctccag 27540 aactgagctg gctgaaatgt tcatgcgtcc tgcacctgag catcttttgc agaagctcat 27600 tgcttctggg gagatcaatc aagaacaagc tgagttggcc agacgtgttc ctgtggctga 27660 tgacattgcg gttgaagctg attctggtgg acacactgac aaccgtccaa tccatgtcat 27720 tctgcctctg attatcaacc tgagggatag gcttcacaga gagtgtggat acccagcaaa 27780 cctgagggtt cgtgttggtg ctgggggtgg cattgggtgt ccccaagctg cgctggcaac 27840 tttcaacatg ggtgcctctt tcattgtcac tggaacagtg aaccaagttg ccaaacaatc 27900 tgggacgtgt gacaatgtga ggaaacagct tgcgaaagca acttactcag atgtttgcat 27960 ggcaccggca gctgacatgt ttgaggaagg tgtgaaactt caagttttga agaaaggaac 28020 aatgttccct tcaagagcca acaaactcta tgagttgttc tgcaagtatg attcttttga 28080 gtccatgcct ccagcagagc ttgcgagggt tgagaaaaga atcttcagtc gtgcattgga 28140 agaggtctgg gatgagacca agaacttcta catcaaccgt ctccacaacc cagagaagat 28200 acagagggct gagagggacc ccaaactgaa gatgtcactg tgtttcagat ggtatctgtc 28260 cctggcttca aggtgggcca acactggagc ttctgacaga gtcatggact accaagtttg 28320 gtgtggtcct gccattggtt cattcaatga tttcatcaaa ggaacttacc ttgatccggc 28380 agtggcaaat gagtacccgt gtgttgtgca gatcaacaaa cagattctta gaggagcgtg 28440 cttcttgagg cgtcttgaaa tactgagaaa tgcaaggctt tcagatggtg cagctgcact 28500 tgtggcgagc attgatgaca catatgtccc tgctgagaag ctgtgagtag ttagcttaat 28560 cacttaggtc acctcgagta tcaaaatcta tttagaaata cacaatattt tgttgcaggc 28620 ttgctggaga atcgatctgc tatcataaaa attacaaaaa aattttattt gcctcaatta 28680 ttttaggatt ggtattaagg acgcttaaat tatttgtcgg gtcactacgc atcattgtga 28740 ttgagaagat cagcgatacg aaatattcgt agtactatcg ataatttatt tgaaaattca 28800 taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata aagccacgca 28860 catttaggat attggccgag attactgaat attgagtaag atcacggaat ttctgacagg 28920 agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc catatgcagg 28980 agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca aggttggcgc 29040 gccgaattcg cggccgctta attaatctga cctgaccact agacctgagt gagtacagat 29100 gtgctactag tgctagcctc gaggtcgacc aacttttcta tacaaagttg tctagaccta 29160 ggactaggca ctgagtgact cactgacctg actgagtgac tggcggccgc ctcccagtat 29220 cattatagtg aaagttttgg ctctctcgcc ggtggttttt tacctctatt taaaggggtt 29280 ttccacctaa aaattctggt atcattctca ctttacttgt tactttaatt tctcataatc 29340 tttggttgaa attatcacgc ttccgcacac gatatcccta caaatttatt atttgttaaa 29400 cattttcaaa ccgcataaaa ttttatgaag tcccgtctat ctttaatgta gtctaacatt 29460 ttcatattga aatatataat ttacttaatt ttagcgttgg tagaaagcat aatgatttat 29520 tcttattctt cttcatataa atgtttaata tacaatataa acaaattctt taccttaaga 29580 aggatttccc attttatatt ttaaaaatat atttatcaaa tatttttcaa ccacgtaaat 29640 ctcataataa taagttgttt caaaagtaat aaaatttaac tccataattt ttttattcga 29700 ctgatcttaa agcaacaccc agtgacacaa ctagccattt ttttctttga ataaaaaaat 29760 ccaattatca ttgtattttt tttatacaat gaaaatttca ccaaacaatg atttgtggta 29820 tttctgaagc aagtcatgtt atgcaaaatt ctataattcc catttgacac tacggaagta 29880 actgaagatc tgcttttaca tgcgagacac atcttctaaa gtaattttaa taatagttac 29940 tatattcaag atttcatata tcaaatactc aatattactt ctaaaaaatt aattagatat 30000 aattaaaata ttactttttt aattttaagt ttaattgttg aatttgtgac tattgattta 30060 ttattctact atgtttaaat tgttttatag atagtttaaa gtaaatataa gtaatgtagt 30120 agagtgttag agtgttaccc taaaccataa actataagat ttatggtgga ctaattttca 30180 tatatttctt attgctttta ccttttcttg gtatgtaagt ccgtaactgg aattactgtg 30240 ggttgccatg acactctgtg gtcttttggt tcatgcatgg atcttgcgca agaaaaagac 30300 aaagaacaaa gaaaaaagac aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa 30360 ttagtcactg gctgatcaag atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa 30420 cacgtgctta acatgcactt taaatggctc acccatctca acccacacac aaacacattg 30480 cctttttctt catcatcacc acaaccacct gtatatattc attctcttcc gccacctcaa 30540 tttcttcact tcaacacacg tcaacctgca tatgcgtgtc atcccatgcc caaatctcca 30600 tgcatgttcc aaccaccttc tctcttatat aatacctata aatacctcta atatcactca 30660 cttctttcat catccatcca tccagagtac tactactcta ctactataat accccaaccc 30720 aactcatatt caatactact ctaggatcca caaccaaaaa tggcgcttcg tgtcaagacc 30780 aacaagaagc catgttggga gatgaccaag gaggaactga cctctggcaa aactgaggtg 30840 ttcaactatg aggaactcct ggagtttgca gagggtgaca tagctaaggt ttttggtccg 30900 gagtttgctg tcatagacaa gtaccccaga agggtgaggc tgcctgctcg tgaatacctg 30960 cttgtgacaa gggtcacact catggatgcc gaggtcaaca actacagagt tggtgccaga 31020 atggtcactg agtatgatct tccagtgaat ggcgaattgt ctgagggagg tgattgtccc 31080 tgggcagttc tggttgagag tggacagtgc gatttgatgc tgatatcata catgggaatt 31140 gatttccaga accaaggtga cagagtttac cgtctgctca acaccacgtt gacattctat 31200 ggggttgccc atgagggaga gacccttgag tatgacatca gagtgaccgg ttttgccaaa 31260 cgtttggatg gtggcatctc catgttcttc tttgagtatg actgctatgt caatgggagg 31320 cttctcattg agatgagaga tggctgtgct ggcttcttca caaatgagga gcttgatgcg 31380 ggaaagggtg tggtgttcac aagaggggat ttggctgcca gagctaagat tccaaagcaa 31440 gatgtttctc cctatgctgt cgctccctgc cttcacaaaa caaaactcaa cgaaaaggag 31500 atgcagacct tggttgacaa agattgggct tccgtttttg gctcaaagaa tggcatgccg 31560 gagatcaact acaaactctg cgcacgtaag atgctcatga ttgatagagt caccagcatt 31620 gaccataagg gaggtgtgta cggtttgggt cagttggttg gtgagaaaat cttggagagg 31680 gaccattggt acttcccatg ccattttgtt aaggatcaag tcatggctgg atctcttgtc 31740 agcgacggtt gttcacaaat gctcaagatg tacatgatct ggctcggttt gcaccttacc 31800 accggaccat ttgacttcag acccgtcaac ggacacccga acaaagttag gtgtaggggt 31860 cagatatctc ctcataaggg aaagttggtc tacgtcatgg agatcaaaga gatgggattc 31920 gacgaggata acgatccgta cgccattgct gacgtcaaca tcattgacgt tgatttcgaa 31980 aagggtcaag atttcagcct tgatagaatc agcgactacg gaaaggggga tcttaacaaa 32040 aagatcgtcg tggacttcaa gggcattgct ctcaagatgc agaagagatc cacgaacaag 32100 aacccctcca aagttcagcc cgtttttgct aacggtgcag ccactgtggg accggaagcg 32160 tccaaggcca gctctggtgc cagtgctagt gcttctgctg cccctgcgaa gcctgctttc 32220 tcagcagatg ttcttgcgcc aaagccagtt gcacttcctg aacatatcct caagggtgac 32280 gctctcgctc cgaaagaaat gtcctggcat cccatgtcaa aacttcctgg caacccgacg 32340 ccgttcttct cccctagttc ttaccctccg agggcaattt gcttcatccc tttccctggc 32400 aacccacttg acaacaattg caaagctgga gaaatgcctc tgaactggta caacatgtca 32460 gagttcatgt gtggaaaagt tagcaactgc ttgggaccag agttcgcaag atttgataag 32520 tcaaatacga gcagaagccc tgcttttgat ttggctcttg tgactagagt tgttgaagtc 32580 acaaacatgg aacatggcaa gttcctgaat gttgattgca atcccagcaa aggcacaatg 32640 gtgggggagt ttgactgtcc tcaagatgcg tggttctttg atggttcttg caatgatggg 32700 cacatgcctt attccatcat catggaaatc ggacttcaga catccggtgt tctcaccagt 32760 gtgttgaaag caccactgac gatggacaaa gatgacattc tctttaggaa ccttgatgca 32820 agtgctgaaa tggtgaggcc agatgtggat gtgaggggga aaaccataag gaatgtgacc 32880 aagtgcactg gatatgcaat gttgggaaag atggggattc acagattcac gtttgagttg 32940 tctgttgatg gcgtggtttt ctacaaagga agcacttcct ttggatggtt cactccagag 33000 gtgtttgctc agcaagctgg acttgacaat gggaaaaaga cggagccttg gtgcaaaact 33060 aacaacacga gtgtgagaag agttgaaatc gcatcagcca aaggcaaaga acagctgact 33120 gagaaacttc cagatgcaac taatgctcaa gttctcagaa ggtcagaaca gtgtgagtat 33180 ttggattacc tcaacattgc tcctgattct gggctgcatg ggaaaggata tgcgcatgga 33240 cacaaagatg ttaacccaca agactggttc ttctcttgtc acttttggtt tgatcctgtg 33300 atgcctggat ctttgggaat tgagtcaatg ttccagctca ttgaggcatt tgcggtggac 33360 cagaacatac cgggagaata caatgtgagc aatccgacct ttgcccatgc ccctggaaaa 33420 acggcgtgga agtaccgtgg tcaattgaca ccaaagaacc gtgcgatgga ctgtgaggtg 33480 cacatagttt caatcacagc ctctcctgaa aatggaggct acgttgacat agtggcagat 33540 ggagcacttt gggttgatgg tctccgtgtg tatgaagcca aagaactcag agttcgtgtt 33600 gtcagtgcaa aacctcaagc cattccggat gttcaacaac agccacccag cgcaaaagcg 33660 gaccctggga aaactggagt tgcactcagt ccgactcagt tgagagatgt cctgcttgaa 33720 gtggacaatc cattgtatct tggtgttgaa aactccaatt tggtgcagtt tgaatccaaa 33780 cctgcaactt cttcaaggat tgtcagtatc aaaccatgct ccataagtga tcttggtgac 33840 aagtccttca tggaaacgta caatgtgtca gcacctctgt acactggagc aatggccaag 33900 ggaattgcgt ctgcggacct tgtcattgct gctgggaaga ggaagatatt gggttctttt 33960 ggagcgggtg ggcttccaat gcaccatgtg agggcagctt tggagaagat acaagcggct 34020 ctgcctcaag gaccatatgc tgtgaacctc attcattcac cttttgacag caacttggaa 34080 aaggggaatg ttgatctctt cctcgaaaag ggcgttactg tggtggaggc ttctgcattc 34140 atgaccctca ctccccaagt tgttcgttac cgtgcagccg gactctcaag gaacgctgat 34200 gggagtgtca acatcagaaa ccgtatcatt ggaaaagtgt ccagaacaga actcgccgaa 34260 atgttcatca gaccggctcc ggaacacctc cttgagaaac tcatagcttc tggtgagata 34320 acacaagaac aagccgaact tgcccgtcgt gttcctgtcg cagacgacat cgctgttgaa 34380 gctgattctg gtggtcacac tgacaaccgt cccatccacg tcatcttgcc tttgatcatc 34440 aaccttagga acagactgca cagagagtgt ggttatcctg cgcaccttag agtcagagtt 34500 ggagctggag gcggtgttgg ctgcccacaa gctgctgcgg ctgccctcac aatgggagct 34560 gccttcatag tgactgggac tgtcaatcaa gttgctaaac agtccgggac atgtgacaac 34620 gtgagaaaac aactcagtca agccacctac agtgacattt gcatggctcc agcagctgat 34680 atgtttgagg aaggtgtgaa actccaagtt ctcaagaagg gaacaatgtt cccatcaaga 34740 gctaacaaat tgtacgaact cttttgcaag tacgactctt tcgattcaat gcctcctgct 34800 gaacttgaaa ggatcgagaa gaggatcttc aagagggcac ttcaagaggt ctgggaagag 34860 accaaggact tttacatcaa tggtttgaag aatccagaga agatacagag agcggaacat 34920 gaccccaaac tcaagatgtc tttgtgtttc agatggtatc ttggtcttgc atcacgttgg 34980 gccaacatgg gtgctcctga tcgtgtcatg gactatcaag tctggtgtgg tccagccatt 35040 ggagccttca atgacttcat caaaggaaca tatcttgatc ctgctgtctc taatgagtac 35100 ccatgtgttg tccagatcaa ccttcagatt ttgcgtggtg catgttacct gaggcgtctt 35160 aatgctctga gaaatgatcc gaggattgat cttgagactg aagatgctgc ctttgtctat 35220 gagccaacga atgcgctttg agtagttagc ttaatcactt aggtcacctc gagtatcaaa 35280 atctatttag aaatacacaa tattttgttg caggcttgct ggagaatcga tctgctatca 35340 taaaaattac aaaaaaattt tatttgcctc aattatttta ggattggtat taaggacgct 35400 taaattattt gtcgggtcac tacgcatcat tgtgattgag aagatcagcg atacgaaata 35460 ttcgtagtac tatcgataat ttatttgaaa attcataaga aaagcaaacg ttacatgaat 35520 tgatgaaaca atacaaagac agataaagcc acgcacattt aggatattgg ccgagattac 35580 tgaatattga gtaagatcac ggaatttctg acaggagcat gtcttcaatt cagcccaaat 35640 ggcagttgaa atactcaaac cgccccatat gcaggagcgg atcattcatt gtttgtttgg 35700 ttgcctttgc caacatggga gtccaaggtt ggcgcgccga attcgcggcc gcttaattaa 35760 tcacgagtgg atgactgtca cgactgactc atgactgact actagtgcta gcctcgaggt 35820 cgacacaact ttgtataata aagttgtcta gacctaggag ctgtgctggg taactcacat 35880 gagtcactgg tcacatcagc ggccgcctcc cagtatcatt atagtgaaag ttttggctct 35940 ctcgccggtg gttttttacc tctatttaaa ggggttttcc acctaaaaat tctggtatca 36000 ttctcacttt acttgttact ttaatttctc ataatctttg gttgaaatta tcacgcttcc 36060 gcacacgata tccctacaaa tttattattt gttaaacatt ttcaaaccgc ataaaatttt 36120 atgaagtccc gtctatcttt aatgtagtct aacattttca tattgaaata tataatttac 36180 ttaattttag cgttggtaga aagcataatg atttattctt attcttcttc atataaatgt 36240 ttaatataca atataaacaa attctttacc ttaagaagga tttcccattt tatattttaa 36300 aaatatattt atcaaatatt tttcaaccac gtaaatctca taataataag ttgtttcaaa 36360 agtaataaaa tttaactcca taattttttt attcgactga tcttaaagca acacccagtg 36420 acacaactag ccattttttt ctttgaataa aaaaatccaa ttatcattgt atttttttta 36480 tacaatgaaa atttcaccaa acaatgattt gtggtatttc tgaagcaagt catgttatgc 36540 aaaattctat aattcccatt tgacactacg gaagtaactg aagatctgct tttacatgcg 36600 agacacatct tctaaagtaa ttttaataat agttactata ttcaagattt catatatcaa 36660 atactcaata ttacttctaa aaaattaatt agatataatt aaaatattac ttttttaatt 36720 ttaagtttaa ttgttgaatt tgtgactatt gatttattat tctactatgt ttaaattgtt 36780 ttatagatag tttaaagtaa atataagtaa tgtagtagag tgttagagtg ttaccctaaa 36840 ccataaacta taagatttat ggtggactaa ttttcatata tttcttattg cttttacctt 36900 ttcttggtat gtaagtccgt aactggaatt actgtgggtt gccatgacac tctgtggtct 36960 tttggttcat gcatggatct tgcgcaagaa aaagacaaag aacaaagaaa aaagacaaaa 37020 cagagagaca aaacgcaatc acacaaccaa ctcaaattag tcactggctg atcaagatcg 37080 ccgcgtccat gtatgtctaa atgccatgca aagcaacacg tgcttaacat gcactttaaa 37140 tggctcaccc atctcaaccc acacacaaac acattgcctt tttcttcatc atcaccacaa 37200 ccacctgtat atattcattc tcttccgcca cctcaatttc ttcacttcaa cacacgtcaa 37260 cctgcatatg cgtgtcatcc catgcccaaa tctccatgca tgttccaacc accttctctc 37320 ttatataata cctataaata cctctaatat cactcacttc tttcatcatc catccatcca 37380 gagtactact actctactac tataataccc caacccaact catattcaat actactctag 37440 gatccacaac caaaaatgct tcagcacact tggcttccga agcctcccaa tctgaccctc 37500 ttgtcagatg aggttcatct ctggaggatt cctcttgacc agcctgagtc acaacttcaa 37560 gaccttgctg ccaccctgag cagtgatgaa ttggcgaggg caaacagatt ctactttcca 37620 gaacacagaa ggcgtttcac tgctgggaga ggcatcctca gatccatctt gggtggatac 37680 ttgggagtgg aaccgggtca agtcaagttt gattatgagt cccgtgggaa accgatcctt 37740 ggtgacagat ttgctgagag tggactcctg ttcaacttgt ctcacagcca gaaccttgcc 37800 ttgtgtgctg tcaactacac gcgtcaaata ggcattgatc ttgaatatct gcgtccaaca 37860 tctgacttgg agtctcttgc aaagaggttc tttctcccaa gagaatatga actcttgagg 37920 tcactccctg atgagcagaa acagaagatt ttctttcgtt actggacttg caaagaggct 37980 tatctcaaag caacgggaga tggaatagcc aaacttgaag agatcgagat agcactcacc 38040 ccaacagaac ctgccaagct ccaaacagct cctgcgtggt ctctgttgga gcttgtgcca 38100 gatgacaatt gtgttgcagc tgtggctgtt gcgggttttg gttggcagcc caagttctgg 38160 cattactgag tagttagctt aatcacttag gtcacctcga gtatcaaaat ctatttagaa 38220 atacacaata ttttgttgca ggcttgctgg agaatcgatc tgctatcata aaaattacaa 38280 aaaaatttta tttgcctcaa ttattttagg attggtatta aggacgctta aattatttgt 38340 cgggtcacta cgcatcattg tgattgagaa gatcagcgat acgaaatatt cgtagtacta 38400 tcgataattt atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat 38460 acaaagacag ataaagccac gcacatttag gatattggcc gagattactg aatattgagt 38520 aagatcacgg aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat 38580 actcaaaccg ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca 38640 acatgggagt ccaaggttgg cgcgccgaat tcgcggccgc ttaattaact actgtcactg 38700 aggccgtaga cgagtacgga ctgatctaac tagtgctagc ctcgaggtcg acacccagct 38760 tt 38762 <210> 49 <211> 35836 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7380 <400> 49 cttgtacaaa gtggttgcgg ccgcttaatt aaatttaaat tcaattaatg caatcttgat 60 tttcaacaac gaaggtaatg gcgtaaaaga aaaaatgtat gttattgtat tgatctttca 120 tgatgttgaa gcgtgccata atatgatgat gtataattaa aatattaact gtcgcatttt 180 attgaaatgg cactgttatt tcaaccatat ctttgattct gttacatgac acgactgcaa 240 gaagtaaata atagacgccg ttgttaaaga attgctatca tatgtgccta actagaggga 300 atttgagcgt cagacctaat caaatattac aaaatatctc actctgtcgc cagcaatggt 360 gtaatcagcg cagacaaatg gcgtaaagat cgcggaaaaa cctccccgag tggcatgata 420 gctgcctctg tattgctgat ttagtcagcc ttatttgact taagggtgcc ctcgttagtg 480 acaaattgct ttcaaggaga cagccatgcc ccacactttg ttgaaaaaca aattgccttt 540 ggggagacgg taaagccagt tgctcttcaa taaggaatgt cgaggaggca atgtaaccgc 600 ctctggtagt acacttctct aatccaaaaa tcaatttgta ttcaagatac cgcaaaaaac 660 ttatggttta aaccctgcag gactagtcca gaaggtaatt atccaagatg tagcatcaag 720 aatccaatgt ttacgggaaa aactatggaa gtattatgta agctcagcaa gaagcagatc 780 aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat acaagatcct 840 atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc aggcgaagaa 900 aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat aaggtcggtg 960 attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg gaaagtaacc 1020 ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc gtgtcatttt 1080 tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac aagaaaaaat 1140 ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa tttgtaagtt 1200 tgtaggtacc agatctggat cccaaaccat gtctccggag aggagaccag ttgagattag 1260 gccagctaca gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac 1320 gtctacagtg aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga 1380 gaggttgcaa gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat 1440 tgcttacgct gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt 1500 ttacgtgtca cataggcatc aaaggttggg cctaggatct acattgtaca cacatttgct 1560 taagtctatg gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga 1620 tccatctgtt aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc 1680 tggatacaag catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc 1740 agctcctcca aggccagtta ggccagttac ccaaatctga gtagttagct taatcaccta 1800 gagctcgatc ggcggcaata gcttcttagc gccatcccgg gttgatccta tctgtgttga 1860 aatagttgcg gtgggcaagg ctctctttca gaaagacagg cggccaaagg aacccaaggt 1920 gaggtgggct atggctctca gttccttgtg gaagcgcttg gtctaaggtg cagaggtgtt 1980 agcgggatga agcaaaagtg tccgattgta acaagatatg ttgatcctac gtaaggatat 2040 taaagtatgt attcatcact aatataatca gtgtattcca atatgtacta cgatttccaa 2100 tgtctttatt gtcgccgtat gtaatcggcg tcacaaaata atccccggtg actttctttt 2160 aatccaggat gaaataatat gttattataa tttttgcgat ttggtccgtt ataggaattg 2220 aagtgtgctt gaggtcggtc gccaccactc ccatttcata attttacatg tatttgaaaa 2280 ataaaaattt atggtattca atttaaacac gtatacttgt aaagaatgat atcttgaaag 2340 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa 2400 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc 2460 tggtacattg ccgtagatga aagactgagt gcgatattat ggtgtaatac ataggaattc 2520 gtttaaacga tctgcgtcta attttcggtc caacttgcac aggaaagacg tcgaccgcgg 2580 tagctcttgc ccagcagact gggcttccag tcctttcgct cgatcgggtc caatgttgtc 2640 ctcagctgtg aaccggaagc ggacgaccaa cagtggaaga actgaaagga acgagccgtc 2700 tataccttga tgatcggcct ctggtgaagg gtatcatcgc agccaagcaa gctcatgaaa 2760 ggctgatggg ggaggtgtat aattatgagg cccacggcgg gcttattctt tagggaggat 2820 ctatctcgtt gctcaagtgc atggcgcaaa gcagttattg gagtgcggat tttcgttggc 2880 atattattcg ccacgagtta gcagacgaag agaccttcat gaacgtggcc aaggccagag 2940 ttaagcagat gttacgccct gctgcaggcc tttctattat ccaatagttg gttgatcttt 3000 ggaaagagcc tcggctgagg cccatactga aagagatcga tggatatcga tatgccatgt 3060 tgtttgctag ccagaaccag atcacatccg atatgctatt gcagcttgac gcagatatgg 3120 aggataagtt gattcatggg atcgctcagg agtagctcat ccatgcacgc cgacaagaac 3180 agaaattccg tcgagttaac gcagccgctt acgacggatt cgaaggtcat ccattcggaa 3240 tgtattagtt tgcaccagct ccgcgtcaca cctgtcttca tttgaataag atgttagcaa 3300 ttgtttttag ctttgtcttg ttgtggcagg gcggcaagtg cttcagacat cattctgttt 3360 tcaaatttta tgctggagaa cagcttctta attcctttgg aaataataga ctgcgtctta 3420 aaattcagat gtctggatat agatatgatt gtaaaataac ctatttaagt gtcatttaga 3480 acataagttt tatgaatgtt cttccatttt cgtcatcgaa cgaataagag taaatacacc 3540 ttttttaaca ttacaaataa gttcttatac gttgtttata caccgggaat catttccatt 3600 attttcgcgc aaaagtcacg gatattcgtg aaagcgacat aaactgcgaa atttgcgggg 3660 agtgtcttga gtttgcctcg aggctagcgc atgcacatag acacacacat catctcattg 3720 atgcttggta ataattgtca ttagattgtt tttatgcata gatgcactcg aaatcagcca 3780 attttagaca agtatcaaac ggatgtgact tcagtacatt aaaaacgtcc gcaatgtgtt 3840 attaagttgt ctaagcgtca atttgattta caattgaata tatcctgccc cagccagcca 3900 acagctcgat ttacaattga atatatcctg ccggccggcc cacgcgtgtc gaggaattct 3960 gatctggccc ccatttggac gtgaatgtag acacgtcgaa ataaagattt ccgaattaga 4020 ataatttgtt tattgctttc gcctataaat acgacggatc gtaatttgtc gttttatcaa 4080 aatgtacttt cattttataa taacgctgcg gacatctaca tttttgaatt gaaaaaaaat 4140 tggtaattac tctttctttt tctccatatt gaccatcata ctcattgctg atccatgtag 4200 atttcccgga catgaagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4260 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4320 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4380 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4440 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 4500 caggtacaat cgagccgacg ttcacgcgga acgaccaagc aagcttggct gccatttttg 4560 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 4620 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 4680 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 4740 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 4800 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 4860 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 4920 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 4980 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 5040 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 5100 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 5160 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 5220 gcggcgaggg caaccagccc ggtgagcgtc ggaaagggtc gacggatctt ttccgctgca 5280 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 5340 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 5400 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 5460 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc 5520 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc 5580 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg 5640 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg 5700 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc 5760 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat 5820 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc 5880 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg 5940 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc 6000 ggagctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 6060 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 6120 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 6180 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 6240 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 6300 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 6360 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 6420 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 6480 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 6540 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 6600 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 6660 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 6720 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 6780 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 6840 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 6900 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 6960 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 7020 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 7080 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 7140 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 7200 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 7260 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 7320 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 7380 tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 7440 ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 7500 gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc 7560 cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag 7620 aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac 7680 atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct 7740 cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc 7800 ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt 7860 ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc 7920 tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc 7980 gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga 8040 ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca 8100 ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg 8160 gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc 8220 gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg 8280 ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga 8340 gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt 8400 tccttactgg gctttctcag cccccgagcg cttagtggga atttgtaccc cttatcgaac 8460 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct 8520 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 8580 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 8640 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 8700 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 8760 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 8820 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 8880 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 8940 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 9000 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 9060 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 9120 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 9180 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac 9240 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag 9300 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 9360 tcgcggcgcg gcttaactca agcgttagag agctggggaa gactatgcgc gatctgttga 9420 aggtggttct aagcctcgtc ttgcgatggc atttcgatcc attcccattc cgcgctcaag 9480 atggcttccc ctcggcagtt catcagggct aaatcaatct agccgacttg tccggtgaaa 9540 tgggctgcac tccaacagaa acaatcaaac aaacatacac agcgacttat tcacacgagc 9600 tcaaattaca acggtatata tcctgccagt cagcatcatc acaccaaaag ttaggcccga 9660 atagtttgaa attagaaagc tcgcaattga ggtctacagg ccaaattcgc tcttagccgt 9720 acaatattac tcaccggatc ctaaccggtt taattaaggc gcgccatgcc cgggcaagcg 9780 gccgcacaag tttgtacaaa aaagcaggct tctaggatct gcttttacat gcgagacaca 9840 tcttctaaag taattttaat aatagttact atattcaaga tttcatatat caaatactca 9900 atattacttc taaaaaatta attagatata attaaaatat tactttttta attttaagtt 9960 taattgttga atttgtgact attgatttat tattctacta tgtttaaatt gttttataga 10020 tagtttaaag taaatataag taatgtagta gagtgttaga gtgttaccct aaaccataaa 10080 ctataagatt tatggtggac taattttcat atatttctta ttgcttttac cttttcttgg 10140 tatgtaagtc cgtaactgga attactgtgg gttgccatga cactctgtgg tcttttggtt 10200 catgcatgga tcttgcgcaa gaaaaagaca aagaacaaag aaaaaagaca aaacagagag 10260 acaaaacgca atcacacaac caactcaaat tagtcactgg ctgatcaaga tcgccgcgtc 10320 catgtatgtc taaatgccat gcaaagcaac acgtgcttaa catgcacttt aaatggctca 10380 cccatctcaa cccacacaca aacacattgc ctttttcttc atcatcacca caaccacctg 10440 tatatattca ttctcttccg ccacctcaat ttcttcactt caacacacgt caacctgcat 10500 atgcgtgtca tcccatgccc aaatctccat gcatgttcca accaccttct ctcttatata 10560 atacctataa atacctctaa tatcactcac ttctttcatc atccatccat ccagagtact 10620 actactctac tactataata ccccaaccca actcatattc aatactactc taggatccac 10680 aaccaaaaat ggcagcgagg ctgcaagagc agaaaggagg tgagatggac acgaggattg 10740 cgatcatagg catgagcgcc atacttcctt gtggaaccac ggtgagagag tcatgggaga 10800 ccataagggc tggcattgat tgcctgtctg atcttcctga ggatcgtgtt gatgtgactg 10860 cgtactttga cccagtcaag accacaaagg acaaaatcta ctgcaagaga ggaggtttca 10920 ttcctgagta tgactttgat gcgcgtgagt ttggtctcaa catgttccag atggaagatt 10980 ctgatgcgaa tcagaccatc tcacttctca aggtcaaaga agcacttcaa gatgctggga 11040 ttgatgcact tggaaaggag aagaaaaaca taggatgcgt gcttggcatt ggaggtggac 11100 agaagagttc acatgagttc tacagcagac tcaactatgt ggttgtggag aaagtcttga 11160 gaaagatggg gatgccagag gaagatgtca aagtggctgt ggagaagtac aaagcgaact 11220 tcccagaatg gagacttgac tctttccctg gattccttgg gaatgtcact gctggcagat 11280 gcaccaacac attcaacttg gatggcatga actgcgttgt ggatgctgca tgtgcaagct 11340 ccttgatagc agtcaaggtt gcgattgatg aactgctcta tggtgactgc gacatgatgg 11400 tcactggtgc aacctgcaca gacaactcca ttgggatgta catggctttc tcaaagacac 11460 ctgtgttcag cacggaccca agtgtgagag cgtatgatga gaaaacgaaa gggatgctca 11520 ttggtgaggg atcagccatg ttggtgctca aacgttatgc agatgcagtg agggatggtg 11580 atgagattca tgctgtgatt cgtggttgtg caagctcaag tgatggcaaa gcagctggca 11640 tctacactcc cacaatcagt ggtcaagagg aagccttgag acgtgcatac aatcgtgcct 11700 gcgttgaccc tgccactgtc actttggttg agggtcatgg gactggcact ccagttggag 11760 ataggattga gttgactgcg ttgaggaact tgtttgacaa agcgtatggt gaagggaaca 11820 cagagaaagt tgcagttgga agtatcaagt ccagtattgg tcatttgaag gctgttgctg 11880 gtcttgctgg aatgatcaag gtcatcatgg ctctcaaaca caagactttg cctggaacga 11940 tcaatgtgga caatccaccc aatctctatg acaacactcc catcaatgag tcatccctct 12000 acatcaacac gatgaacaga ccctggttcc ctccacctgg tgttccgaga cgtgctggaa 12060 tatccagctt tggctttgga ggtgccaact accatgcagt gttggaagag gctgaaccag 12120 agcacacgac agcgtacaga ctcaacaaac gtccacaacc ggtgttgatg atggctgcaa 12180 ctcctgctgc gttgcagagc ctttgtgaag ctcagctgaa agagtttgaa gctgcgatca 12240 aggagaatga gacagtcaag aatactgcct acatcaagtg tgtcaagttt ggagaacagt 12300 tcaagttccc tgggagcata cctgcgacca atgcgagact gggattcttg gtcaaagatg 12360 cggaggatgc gtgctccacg cttcgtgcta tatgtgctca gtttgccaaa gatgtgacca 12420 aagaagcgtg gaggcttccg agagaaggag tttctttccg tgccaaaggc attgccacca 12480 atggtgctgt tgctgcactc ttctctggac aaggtgctca gtacacacac atgttcagtg 12540 aagttgcgat gaactggcct cagttcagac agtccattgc tgcaatggac gctgcacagt 12600 ccaaggttgc tggaagtgac aaagactttg aaagagtctc tcaagtcctg tacccaagga 12660 aaccgtacga acgtgagcca gaacaagatc acaagaaaat ctcactcaca gcatacagcc 12720 agccgtcaac cctggcatgt gctcttggtg cctttgagat tttcaaggaa gctggattca 12780 ctccagactt tgcagctgga cacagcttgg gtgagtttgc tgcgctctat gcagctggtt 12840 gtgttgacag agatgagttg tttgaacttg tttgcagaag ggcaaggatc atgggaggca 12900 aagatgcacc tgccacaccc aaaggttgca tggcagctgt cataggaccc aacgcagaga 12960 acatcaaggt tcaagctgcc aacgtctggc ttggaaactc aaactctccg tctcagactg 13020 tcattactgg gtctgttgaa gggattcaag ctgaaagtgc cagattgcag aaggaagggt 13080 tcagagttgt gcctcttgct tgtgagtctg ccttccattc tccacagatg gaaaatgcca 13140 gctcagcttt caaagatgtc atatccaagg tttccttccg tacacccaaa gcagaaacaa 13200 agctcttcag caatgtttct ggagagacct atccgacgga tgccagagag atgctcactc 13260 aacacatgac ctcatctgtg aagttcctga cccaagtgag gaacatgcac caagctggtg 13320 cgagaatctt tgttgagttt ggaccgaaac aagttctcag caaacttgtt tctgaaaccc 13380 tcaaagatga cccgtctgtt gtcacagtgt ctgtgaaccc agcttctggc acggactctg 13440 acatacagtt gagggatgct gcagttcagc tggttgtggc tggtgtcaat cttcaagggt 13500 ttgacaagtg ggatgctcct gatgccacga ggatgcaagc catcaagaaa aagagaacca 13560 cactcagact ttcagctgcc acctatgtca gtgacaagac caagaaagtg agggatgctg 13620 ccatgaacga cggaagatgt gtcacatacc tcaagggtgc tgcaccactc atcaaggctc 13680 ctgaacctgt ggttgacgaa gctgccaaga gggaggctga aaggcttcag aaggagcttc 13740 aagacgctca gaggcagctt gacgacgcca aacgtgcagc tgcagaagcc aactccaaac 13800 ttgctgctgc caaggaggaa gccaagactg ctgcagcttc agccaagcct gcagttgaca 13860 ctgctgttgt ggagaaacat cgtgccatcc tcaagtccat gctggctgag ctggatggtt 13920 atggaagtgt tgatgcttct tcccttcagc aacagcaaca gcaacagact gctccggcgc 13980 ctgttaaagc cgctgcccct gccgctcccg ttgcaagtgc tcctgctccc gccgtgtcca 14040 acgagttgct tgagaaagct gagacagttg tgatggaggt tctcgctgcc aagactggat 14100 acgaaacaga catgattgag gcggacatgg agcttgagac tgagttggga attgacagca 14160 tcaagagggt tgaaattctt tctgaagtcc aagctatgct taacgttgag gcgaaagatg 14220 ttgatgctct ctctaggacg agaacagtgg gtgaagttgt caacgctatg aaggcagaaa 14280 tcgctggttc ttccgctccg gcgccggcag ctgccgcacc tgcaccggct aaagcggctc 14340 cagctgcagc tgcaccagct gtttctaatg agctgctcga aaaggcagaa actgttgtga 14400 tggaagtcct tgctgccaaa actggctacg aaactgacat gatagaatca gacatggagc 14460 ttgagacgga gcttggaatt gattccatca agcgtgtgga aatcctcagt gaagtccaag 14520 ccatgctcaa tgtggaagct aaggatgtgg atgctctctc aaggaccaga actgtcggtg 14580 aggttgtcaa tgccatgaag gctgagatag ctggaggctc cgctcctgcg ccggccgctg 14640 ccgctcctgg tcccgctgcc gcggctccag ctccagctgc agccgctccg gccgtctcaa 14700 acgaactcct tgaaaaagct gagaccgtcg tgatggaggt cttggcagct aaaaccggct 14760 atgagactga catgattgag tctgacatgg aattggagac agaacttggg atagacagca 14820 tcaaacgtgt tgaaatcctc agtgaggtcc aagctatgct gaacgtggaa gcgaaggatg 14880 ttgatgctct gtccagaacg aggaccgttg gtgaggttgt ggatgccatg aaggctgaaa 14940 ttgctggagg gtctgcgccc gcgccagctg cagccgctcc tgcacccgca gccgcggcac 15000 ctgctccagc tgcacctgca cctgccgttt cttcagaact cttggagaag gctgagacgg 15060 ttgtgatgga ggttttggct gcaaagactg gctatgagac cgacatgatt gaatctgata 15120 tggaattgga gacagagctt gggatcgatt ccatcaaaag agtggagatc ctgagtgaag 15180 tccaagccat gctcaatgtt gaagctaagg atgttgatgc actttcaaga acaaggacag 15240 tgggtgaggt tgtggatgcc atgaaggcgg agattgctgg agggagcgcg cctgcgccag 15300 ctgccgcggc acctgcaccg gcagccgcag ctccagctcc agctgcacca gcaccggccg 15360 cgcccgcgcc agctgtttca tccgaactcc tggaaaaggc cgagacggtt gtgatggagg 15420 tgctcgcagc taagactggt tacgagaccg acatgattga aagtgacatg gagcttgaaa 15480 ccgagttggg aatagatagc atcaaacgtg ttgaaatctt gtctgaggtc caagctatgt 15540 tgaacgtgga ggcaaaagat gtcgatgcgc tttcaagaac cagaacagtc ggtgaggtcg 15600 tggacgccat gaaggctgaa attgccggaa gctctgctag tgcgccagct gcagccgcac 15660 ctgcacctgc tgccgcggca cctgctccag ctgcagccgc tcctgccgtc agcaatgagt 15720 tgctggagaa agccgaaaca gttgtgatgg aagtccttgc tgcaaaaact ggttacgaaa 15780 cggacatgat tgagtcagac atggaattgg agaccgaact tggaatagat tccatcaaga 15840 gagtggagat cctctctgag gtgcaagcca tgctcaacgt tgaggctaaa gatgttgatg 15900 cactcagtag gacaagaact gtgggggaag ttgtcgatgc gatgaaggct gagattgctg 15960 gaggctctgc tcctgcgccg gcagctgccg cacctgcacc cgcagccgcg gcaccagctg 16020 tcagcaacga attgcttgag aaggctgaaa cagttgtcat ggaagtgctg gctgcaaaaa 16080 ccggctatga gactgacatg attgagtctg acatggaact tgagacggaa ttgggcattg 16140 acagcatcaa gcgtgttgag atcctcagtg aagtccaagc aatgctcaat gtggaggcta 16200 aggatgtgga tgccctctca aggaccagaa cagttggtga ggtcgttgat gcgatgaagg 16260 cggagatcgc tggaagttcc gcgcctgcgc cagctgcagc cgctcctgca cccgcagccg 16320 cggcaccggc tccagctgca gccgcaccgg ccgtctcctc agaactcctg gaaaaggctg 16380 agacagttgt gatggaagtc cttgctgcga aaactggcta tgagaccgac atgattgaat 16440 ctgacatgga actggagacc gagcttggaa ttgactccat caaacgtgtt gaaatcctct 16500 ctgaggttca agcgatgttg aatgtggagg ccaaagatgt tgatgctctt tccagaacaa 16560 ggacggtggg agaggtggtt gatgccatga aggctgagat agctgggggt tctgctcctg 16620 cgccagctgc agccgctcct gctcccgctg ccgcggcacc agctgtcagc aacgaactcc 16680 ttgaaaaagc tgaaactgtt gtgatggagg ttttggctgc aaagactgga tacgagactg 16740 acatgataga gtcagacatg gagttggaaa cagagcttgg gattgatagc atcaagcgtg 16800 tggaaatcct ttctgaggtt caagccatgc tgaacgttga ggccaaagat gtcgatgctt 16860 tgtcaaggac cagaacggtt ggagaagtgg tcgatgccat gaaggctgag atagctggtg 16920 gatcagcccc tgctccggct gcagccgctc ctgcaagtgc cggtgctgca ccagctgtga 16980 agattgattc agttcatggt gctgactgtg acgatttgtc actgatgcac gcaaaggtgg 17040 ttgacatcag aaggccagat gaactgattc tggaaagacc ggagaaccgt ccagttcttg 17100 tcgttgacga tggctcagag ttgacacttg ctcttgtgag ggtgttggga gcctgtgcag 17160 ttgtcttgac ctttgaggga ttgcagttgg ctcagagagc tggtgcagct gccattcgtc 17220 atgttctggc taaggacctt tcagcagaat cagctgagaa ggccatcaaa gaggctgagc 17280 aaagatttgg agcgttggga ggcttcataa gccagcaagc agaaaggttt gaaccagctg 17340 agatactggg tttcacactc atgtgtgcga agtttgccaa agcctctctc tgcactgctg 17400 tggctggagg tcgtcctgcc ttcatcggtg tggctaggct tgacggcaga cttggattca 17460 cctctcaagg cacttctgac gcactcaaga gagcacagcg tggagcgatc tttggactct 17520 gcaagacaat agggttggag tggtcagaat ctgatgtgtt cagccgtggt gtggacattg 17580 ctcaagggat gcacccagaa gatgctgcag ttgccatagt tagggagatg gcgtgtgctg 17640 acatcagaat cagagaggtt gggattggag caaaccagca aagatgcaca atcagagctg 17700 ccaagttgga gactgggaac cctcagagac agatagcgaa agacgatgtg cttctggttt 17760 ctggaggtgc tcgtgggatc acacctcttt gcatccgtga gatcacgcgt cagattgctg 17820 gaggcaagta cattctgttg ggtcgtagca aagtttctgc gtctgaacca gcatggtgtg 17880 ctggcatcac tgatgagaag gctgtgcaga aagctgccac ccaagagttg aagagggcct 17940 tcagtgctgg tgaaggaccc aaacccactc caagggctgt cacgaaactt gtgggatctg 18000 ttcttggtgc tcgtgaagtg agaagctcca ttgctgccat tgaagctctg ggtggcaaag 18060 cgatctacag ctcttgtgat gtgaactctg ctgcagatgt tgccaaagca gttagggatg 18120 cagagtccca gcttggtgcc agagttagtg gaatcgttca cgcttctggt gtgctccgtg 18180 atcgtctcat agagaagaaa ctccctgacg agtttgatgc tgtctttggg accaaagtca 18240 ctgggttgga gaatcttctg gctgcagttg atcgtgccaa cctcaaacac atggttctct 18300 tcagctccct tgctggtttc cacggcaatg ttggtcagtc tgactatgcg atggcaaacg 18360 aagctctcaa caagatgggt ttggaacttg cgaaggatgt cagtgtcaag tccatctgct 18420 ttggtccctg ggatggtggg atggtgacac ctcaactcaa gaaacagttt caagaaatgg 18480 gagttcagat cattcctcgt gaaggaggtg ctgatacagt tgcgaggatt gtgttgggat 18540 caagtcctgc tgagatcctt gttggaaact ggaggacacc atccaagaaa gttggaagtg 18600 acacgatcac cctgcataga aagatcagtg ccaagtccaa tcccttcttg gaggatcatg 18660 tcatacaagg cagaagggtg ttgccaatga cccttgctat tgggagcctt gctgagacct 18720 gccttggact cttccctggc tactctctct gggccataga tgacgcacag ctcttcaaag 18780 gtgtcactgt tgatggagac gtcaactgtg aagtgaccct cacaccgtcc actgctccga 18840 gtgggagggt gaatgtccaa gcgacgctca agacgttctc aagtggaaaa cttgttccag 18900 cctacagagc tgtcatagtg ttgtccaacc aaggtgctcc acctgccaat gcaaccatgc 18960 agcctccgag ccttgatgca gatcctgctt tgcaagggtc agtctatgat ggcaagactc 19020 tctttcatgg tcctgcgttc agaggcattg atgacgttct cagctgcacc aagagccagc 19080 ttgttgccaa gtgttctgct gttcctggat cagatgcagc cagaggagag tttgcaactg 19140 acactgatgc acatgatccc tttgtcaatg atcttgcctt tcaagcaatg ttggtctggg 19200 tgaggagaac gctgggacaa gctgcactcc cgaactccat tcagaggata gtccaacaca 19260 gaccggtccc acaagacaaa cccttctaca tcaccctcag atccaatcag agtggtggac 19320 atagccagca caaacatgca cttcagttcc acaacgaaca aggtgatctc ttcattgatg 19380 ttcaagcctc tgtcattgcg acagactcac ttgcgttctg agtagttagc ttaatcactt 19440 aggtcacctc gagtatcaaa atctatttag aaatacacaa tattttgttg caggcttgct 19500 ggagaatcga tctgctatca taaaaattac aaaaaaattt tatttgcctc aattatttta 19560 ggattggtat taaggacgct taaattattt gtcgggtcac tacgcatcat tgtgattgag 19620 aagatcagcg atacgaaata ttcgtagtac tatcgataat ttatttgaaa attcataaga 19680 aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt 19740 aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat 19800 gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg 19860 atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaaggtt ggcgcgccga 19920 attcgcggcc gcttaattaa gatgagtgat actcaggact caggactcac tctgctgatc 19980 actagtgcta gcctcgaggt cgacacaact ttgtatacaa aagttgtcta ggatctgctt 20040 ttacatgcga gacacatctt ctaaagtaat tttaataata gttactatat tcaagatttc 20100 atatatcaaa tactcaatat tacttctaaa aaattaatta gatataatta aaatattact 20160 tttttaattt taagtttaat tgttgaattt gtgactattg atttattatt ctactatgtt 20220 taaattgttt tatagatagt ttaaagtaaa tataagtaat gtagtagagt gttagagtgt 20280 taccctaaac cataaactat aagatttatg gtggactaat tttcatatat ttcttattgc 20340 ttttaccttt tcttggtatg taagtccgta actggaatta ctgtgggttg ccatgacact 20400 ctgtggtctt ttggttcatg catggatctt gcgcaagaaa aagacaaaga acaaagaaaa 20460 aagacaaaac agagagacaa aacgcaatca cacaaccaac tcaaattagt cactggctga 20520 tcaagatcgc cgcgtccatg tatgtctaaa tgccatgcaa agcaacacgt gcttaacatg 20580 cactttaaat ggctcaccca tctcaaccca cacacaaaca cattgccttt ttcttcatca 20640 tcaccacaac cacctgtata tattcattct cttccgccac ctcaatttct tcacttcaac 20700 acacgtcaac ctgcatatgc gtgtcatccc atgcccaaat ctccatgcat gttccaacca 20760 ccttctctct tatataatac ctataaatac ctctaatatc actcacttct ttcatcatcc 20820 atccatccag agtactacta ctctactact ataatacccc aacccaactc atattcaata 20880 ctactctagg atccacaacc aaaaatggct gcaaggaacg tgagtgcggc tcatgagatg 20940 catgacgaga agaggatagc tgttgtgggc atggctgttc agtatgctgg atgcaaaacg 21000 aaagacgagt tctgggaagt tctcatgaat ggaaaagttg agagcaaagt gatcagcgac 21060 aaacgtcttg gttcaaatta ccgtgctgaa cactacaaag ctgaaaggag caagtatgct 21120 gacaccttct gcaatgagac atatggaact cttgatgaga acgaaataga caatgagcat 21180 gaactccttt tgaaccttgc caaacaagcc ttggctgaaa catctgtcaa agattcaaca 21240 agatgtggca tagtttctgg atgcctcagc ttcccgatgg acaacctcca aggtgaactc 21300 ttgaatgtgt accagaacca tgttgagaag aaacttggtg ccagagtttt caaagatgcc 21360 tctcactggt cagaaagaga acagtccaac aaaccagagg ctggtgacag aagaatcttc 21420 atggaccctg cttcctttgt tgctgaggaa ctcaatcttg gcgctctcca ctactctgtt 21480 gatgctgcct gtgccactgc gctctatgtg ctcagactgg cgcaagatca cttggtctct 21540 ggtgcagctg atgtgatgct ttgtggtgca acatgcctgc cggagccgtt tttcatactc 21600 tctgggttct caaccttcca agccatgcct gttggaacgg gtcagaatgt gtccatgccg 21660 ctgcacaaag attctcaagg attgacccct ggtgaaggag gttccatcat ggtcctcaaa 21720 cgtttggatg acgcaatcag agatggagat cacatctatg gaacactttt gggagccaat 21780 gtcagcaaca gtggcactgg tctgccactt aaaccccttt tgccgtcaga gaaaaagtgc 21840 ctcatggaca cctacacacg tatcaatgtg catccccaca agattcagta tgttgaatgc 21900 catgcaactg ggactccaca aggtgatcgt gtggaaattg atgctgtgaa ggcttgcttt 21960 gaaggcaaag tcccacgttt tggaaccaca aaagggaact ttggacacac attggtggca 22020 gctgggtttg ctggcatgtg caaagtgctt ctctccatga agcacgggat cattccaccc 22080 actccgggaa tagatgacga aacgaagatg gaccctctgg ttgtctctgg tgaggccatt 22140 ccctggccag aaacgaatgg agaaccgaag cgtgctggtc tctcagcgtt tggatttggt 22200 gggaccaatg ctcatgctgt ctttgaggaa catgacccaa gcaatgctgc atgcactggc 22260 catgactcca tttctgctct ctctgcacgt tgtggtggtg agtcaaacat gagaatagca 22320 atcactggga tggatgccac ttttggtgct ttgaaaggat tggatgcttt tgaaagggcc 22380 atctacactg gtgctcatgg tgctatacca ctcccagaaa agagatggag attcctgggg 22440 aaagacaagg acttccttga tctttgtgga gtcaaagcaa caccgcatgg ctgttacatt 22500 gaagatgttg aagttgattt ccagaggctg aggactccaa tgactcctga agatatgctc 22560 ttgcctcaac agcttctggc tgtcaccaca attgataggg ccatattgga ctctggcatg 22620 aagaaaggtg gaaatgtggc tgtctttgtg gggttgggga ccgatcttga actctaccgt 22680 caccgtgcaa gggttgctct caaggagaga gtgaggcctg aagccagcaa gaaactcaat 22740 gacatgatgc agtacatcaa tgattgtggg acaagcactt cttacaccag ctacataggc 22800 aacttggttg caacaagggt cagttcacaa tggggcttca cgggaccttc cttcacaatc 22860 acagaaggca acaattctgt gtacagatgt gctgaacttg gcaaatacct cttggaaacg 22920 ggagaggttg atggggttgt ggttgcgggt gttgatctct gtgggagtgc tgagaacctt 22980 tacgtcaagt caaggcgttt caaggtgtct acatctgaca ccccgagagc cagctttgat 23040 gcagctgctg atggttactt tgttggagag ggctgtggtg catttgtgct caagcgtgag 23100 acaagctgca ccaaggatga cagaatctat gcttgcatgg atgccatagt tcctggaaat 23160 gttccatctg catgtttgag agaggcattg gatcaagcca gagtcaagcc tggagacatc 23220 gagatgttgg agctttctgc tgattcagcg aggcatttga aggacccgtc tgttctccca 23280 aaggaactca ctgctgagga agagattggc ggacttcaga cgattcttcg tgacgatgac 23340 aagctcccaa ggaatgttgc aactggaagt gtcaaggcca ctgttggtga cactggttat 23400 gcttctggtg ctgccagttt gattaaggct gcgctttgca tctacaaccg ttacctgccc 23460 agcaatggag atgactggga tgaacctgca cctgaagcac cctgggacag cacactcttt 23520 gcgtgccaga cctcaagagc ttggttgaag aaccctggtg agagacgtta tgcggctgtt 23580 tccggagtca gtgaaaccag aagttgctac agtgtgctgc tttctgaagc agagggacac 23640 tacgagagag agaacagaat cagtttggat gaggaagcac ccaagctcat tgtgttgaga 23700 gcagactccc atgaagagat tcttggaaga ttggacaaga taagggaaag gttcttgcag 23760 cccacgggtg ctgcacccag agagtctgaa ttgaaggcgc aagcccgtag gatctttctt 23820 gaactccttg gtgagaccct tgcccaagat gcagcttcat ctggaagtca gaagcccctt 23880 gctctcagct tggtctccac tccatccaag ctccagagag aggtggaact tgcggcaaaa 23940 ggcattccga gatgtctcaa gatgagacgt gattggagtt cccctgctgg aagcagatat 24000 gcgcctgagc ctttggccag tgacagagtt gccttcatgt atggagaagg aaggagccct 24060 tactatggca tcacacaaga cattcatagg atttggcctg aacttcatga ggtcatcaat 24120 gagaaaacaa accgtctctg ggctgaaggt gacagatggg tcatgccaag ggccagcttc 24180 aagagtgagc ttgagtcaca acagcaagag tttgacagaa acatgattga aatgttccgt 24240 cttggaatcc tcacctcaat tgccttcaca aacctggcga gagatgttct caacatcacg 24300 cccaaagcag cctttggact cagtttggga gagatttcca tgatctttgc tttctccaag 24360 aaaaacggtt tgataagcga tcagttgaca aaggatttga gagagtcaga tgtctggaac 24420 aaagctctgg ctgttgagtt caatgcgctc agagaagcct ggggcattcc acagtctgtc 24480 ccaaaagatg agttctggca aggttacata gtgaggggaa cgaaacaaga catagaagca 24540 gccatagcac cagactcaaa gtatgtgagg ttgaccatca tcaatgatgc caacactgct 24600 ctcatcagtg gaaagcctga tgcatgcaaa gctgcgattg ctcgtcttgg tggcaacatc 24660 cctgcgcttc cagtcactca aggcatgtgt gggcattgcc cagaggtggg accttacacc 24720 aaggacatcg caaagatcca cgccaacctt gagttccctg tggttgatgg tcttgatctc 24780 tggactacca taaaccagaa gaggcttgtg ccaagagcca cgggtgccaa agatgaatgg 24840 gctccttcat cctttgggga gtatgctgga cagctctatg agaaacaagc caacttccct 24900 cagatagttg aaaccatcta caagcagaac tatgatgtgt ttgttgaagt tgggccaaac 24960 aatcacagaa gcacagcagt gagaaccacg cttggaccac agagaaacca ccttgctggt 25020 gccatagaca agcagaacga ggatgcttgg acgacaatag tcaagctcgt ggcttcattg 25080 aaggctcacc ttgttcctgg agtgaccatc agtccgctct accactccaa gctcgttgca 25140 gaggctgagg cttgttatgc tgcgctctgt aaaggtgaga aacccaagaa aaacaagttt 25200 gtgagaaaga ttcagttgaa tggcagattc aacagcaagg cagaccccat ctcctcagca 25260 gaccttgcat ccttcccacc tgcggaccct gcgattgaag ctgccatctc ttcaaggatc 25320 atgaagcctg tggctccgaa attctatgcg cgtctcaaca ttgatgagca agatgagacc 25380 agagatccga tcttgaacaa ggacaatgcg ccttccagct ccagctcttc cagcagttcc 25440 tcttccagca gttcatctcc gtctcctgct ccttcagctc cagttcagaa gaaagctgcc 25500 ccagctgcgg agacaaaagc tgttgccagt gctgatgcac ttcgtagtgc cctgcttgat 25560 cttgactcca tgcttgcgct cagctctgca agtgcttctg gcaaccttgt tgagactgcg 25620 ccatctgatg ccagtgtcat tgtccctccg tgcaacattg ctgatcttgg cagcagagct 25680 ttcatgaaaa cttatggtgt ttctgcgcct ctgtacaccg gtgcaatggc caagggaatt 25740 gcctctgcgg acttggtgat cgcagctgga aggcaaggaa tccttgcgtc atttggtgct 25800 ggaggccttc caatgcaagt tgtgcgtgag tccattgaaa agattcaagc tgccctgccc 25860 aatggaccat atgctgtcaa cctcatccat tctccttttg acagcaacct tgagaaggga 25920 aatgttgatc tcttcttgga aaagggtgtc acttttgttg aggcttcagc cttcatgacg 25980 ttgacaccgc aagttgtcag atacagagcg gctgggttga cgaggaatgc agatgggtca 26040 gtcaacataa ggaacagaat cattgggaaa gtctccagaa ctgagctggc tgaaatgttc 26100 atgcgtcctg cacctgagca tcttttgcag aagctcattg cttctgggga gatcaatcaa 26160 gaacaagctg agttggccag acgtgttcct gtggctgatg acattgcggt tgaagctgat 26220 tctggtggac acactgacaa ccgtccaatc catgtcattc tgcctctgat tatcaacctg 26280 agggataggc ttcacagaga gtgtggatac ccagcaaacc tgagggttcg tgttggtgct 26340 gggggtggca ttgggtgtcc ccaagctgcg ctggcaactt tcaacatggg tgcctctttc 26400 attgtcactg gaacagtgaa ccaagttgcc aaacaatctg ggacgtgtga caatgtgagg 26460 aaacagcttg cgaaagcaac ttactcagat gtttgcatgg caccggcagc tgacatgttt 26520 gaggaaggtg tgaaacttca agttttgaag aaaggaacaa tgttcccttc aagagccaac 26580 aaactctatg agttgttctg caagtatgat tcttttgagt ccatgcctcc agcagagctt 26640 gcgagggttg agaaaagaat cttcagtcgt gcattggaag aggtctggga tgagaccaag 26700 aacttctaca tcaaccgtct ccacaaccca gagaagatac agagggctga gagggacccc 26760 aaactgaaga tgtcactgtg tttcagatgg tatctgtccc tggcttcaag gtgggccaac 26820 actggagctt ctgacagagt catggactac caagtttggt gtggtcctgc cattggttca 26880 ttcaatgatt tcatcaaagg aacttacctt gatccggcag tggcaaatga gtacccgtgt 26940 gttgtgcaga tcaacaaaca gattcttaga ggagcgtgct tcttgaggcg tcttgaaata 27000 ctgagaaatg caaggctttc agatggtgca gctgcacttg tggcgagcat tgatgacaca 27060 tatgtccctg ctgagaagct gtgagtagtt agcttaatca cttaggtcac ctcgagtatc 27120 aaaatctatt tagaaataca caatattttg ttgcaggctt gctggagaat cgatctgcta 27180 tcataaaaat tacaaaaaaa ttttatttgc ctcaattatt ttaggattgg tattaaggac 27240 gcttaaatta tttgtcgggt cactacgcat cattgtgatt gagaagatca gcgatacgaa 27300 atattcgtag tactatcgat aatttatttg aaaattcata agaaaagcaa acgttacatg 27360 aattgatgaa acaatacaaa gacagataaa gccacgcaca tttaggatat tggccgagat 27420 tactgaatat tgagtaagat cacggaattt ctgacaggag catgtcttca attcagccca 27480 aatggcagtt gaaatactca aaccgcccca tatgcaggag cggatcattc attgtttgtt 27540 tggttgcctt tgccaacatg ggagtccaag gttggcgcgc cgaattcgcg gccgcttaat 27600 taatctgacc tgaccactag acctgagtga gtacagatgt gctactagtg ctagcctcga 27660 ggtcgaccaa cttttctata caaagttgtc taggatctgc ttttacatgc gagacacatc 27720 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 27780 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 27840 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 27900 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 27960 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 28020 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 28080 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 28140 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 28200 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 28260 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 28320 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 28380 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 28440 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 28500 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 28560 ccaaaaatgg cgcttcgtgt caagaccaac aagaagccat gttgggagat gaccaaggag 28620 gaactgacct ctggcaaaac tgaggtgttc aactatgagg aactcctgga gtttgcagag 28680 ggtgacatag ctaaggtttt tggtccggag tttgctgtca tagacaagta ccccagaagg 28740 gtgaggctgc ctgctcgtga atacctgctt gtgacaaggg tcacactcat ggatgccgag 28800 gtcaacaact acagagttgg tgccagaatg gtcactgagt atgatcttcc agtgaatggc 28860 gaattgtctg agggaggtga ttgtccctgg gcagttctgg ttgagagtgg acagtgcgat 28920 ttgatgctga tatcatacat gggaattgat ttccagaacc aaggtgacag agtttaccgt 28980 ctgctcaaca ccacgttgac attctatggg gttgcccatg agggagagac ccttgagtat 29040 gacatcagag tgaccggttt tgccaaacgt ttggatggtg gcatctccat gttcttcttt 29100 gagtatgact gctatgtcaa tgggaggctt ctcattgaga tgagagatgg ctgtgctggc 29160 ttcttcacaa atgaggagct tgatgcggga aagggtgtgg tgttcacaag aggggatttg 29220 gctgccagag ctaagattcc aaagcaagat gtttctccct atgctgtcgc tccctgcctt 29280 cacaaaacaa aactcaacga aaaggagatg cagaccttgg ttgacaaaga ttgggcttcc 29340 gtttttggct caaagaatgg catgccggag atcaactaca aactctgcgc acgtaagatg 29400 ctcatgattg atagagtcac cagcattgac cataagggag gtgtgtacgg tttgggtcag 29460 ttggttggtg agaaaatctt ggagagggac cattggtact tcccatgcca ttttgttaag 29520 gatcaagtca tggctggatc tcttgtcagc gacggttgtt cacaaatgct caagatgtac 29580 atgatctggc tcggtttgca ccttaccacc ggaccatttg acttcagacc cgtcaacgga 29640 cacccgaaca aagttaggtg taggggtcag atatctcctc ataagggaaa gttggtctac 29700 gtcatggaga tcaaagagat gggattcgac gaggataacg atccgtacgc cattgctgac 29760 gtcaacatca ttgacgttga tttcgaaaag ggtcaagatt tcagccttga tagaatcagc 29820 gactacggaa agggggatct taacaaaaag atcgtcgtgg acttcaaggg cattgctctc 29880 aagatgcaga agagatccac gaacaagaac ccctccaaag ttcagcccgt ttttgctaac 29940 ggtgcagcca ctgtgggacc ggaagcgtcc aaggccagct ctggtgccag tgctagtgct 30000 tctgctgccc ctgcgaagcc tgctttctca gcagatgttc ttgcgccaaa gccagttgca 30060 cttcctgaac atatcctcaa gggtgacgct ctcgctccga aagaaatgtc ctggcatccc 30120 atgtcaaaac ttcctggcaa cccgacgccg ttcttctccc ctagttctta ccctccgagg 30180 gcaatttgct tcatcccttt ccctggcaac ccacttgaca acaattgcaa agctggagaa 30240 atgcctctga actggtacaa catgtcagag ttcatgtgtg gaaaagttag caactgcttg 30300 ggaccagagt tcgcaagatt tgataagtca aatacgagca gaagccctgc ttttgatttg 30360 gctcttgtga ctagagttgt tgaagtcaca aacatggaac atggcaagtt cctgaatgtt 30420 gattgcaatc ccagcaaagg cacaatggtg ggggagtttg actgtcctca agatgcgtgg 30480 ttctttgatg gttcttgcaa tgatgggcac atgccttatt ccatcatcat ggaaatcgga 30540 cttcagacat ccggtgttct caccagtgtg ttgaaagcac cactgacgat ggacaaagat 30600 gacattctct ttaggaacct tgatgcaagt gctgaaatgg tgaggccaga tgtggatgtg 30660 agggggaaaa ccataaggaa tgtgaccaag tgcactggat atgcaatgtt gggaaagatg 30720 gggattcaca gattcacgtt tgagttgtct gttgatggcg tggttttcta caaaggaagc 30780 acttcctttg gatggttcac tccagaggtg tttgctcagc aagctggact tgacaatggg 30840 aaaaagacgg agccttggtg caaaactaac aacacgagtg tgagaagagt tgaaatcgca 30900 tcagccaaag gcaaagaaca gctgactgag aaacttccag atgcaactaa tgctcaagtt 30960 ctcagaaggt cagaacagtg tgagtatttg gattacctca acattgctcc tgattctggg 31020 ctgcatggga aaggatatgc gcatggacac aaagatgtta acccacaaga ctggttcttc 31080 tcttgtcact tttggtttga tcctgtgatg cctggatctt tgggaattga gtcaatgttc 31140 cagctcattg aggcatttgc ggtggaccag aacataccgg gagaatacaa tgtgagcaat 31200 ccgacctttg cccatgcccc tggaaaaacg gcgtggaagt accgtggtca attgacacca 31260 aagaaccgtg cgatggactg tgaggtgcac atagtttcaa tcacagcctc tcctgaaaat 31320 ggaggctacg ttgacatagt ggcagatgga gcactttggg ttgatggtct ccgtgtgtat 31380 gaagccaaag aactcagagt tcgtgttgtc agtgcaaaac ctcaagccat tccggatgtt 31440 caacaacagc cacccagcgc aaaagcggac cctgggaaaa ctggagttgc actcagtccg 31500 actcagttga gagatgtcct gcttgaagtg gacaatccat tgtatcttgg tgttgaaaac 31560 tccaatttgg tgcagtttga atccaaacct gcaacttctt caaggattgt cagtatcaaa 31620 ccatgctcca taagtgatct tggtgacaag tccttcatgg aaacgtacaa tgtgtcagca 31680 cctctgtaca ctggagcaat ggccaaggga attgcgtctg cggaccttgt cattgctgct 31740 gggaagagga agatattggg ttcttttgga gcgggtgggc ttccaatgca ccatgtgagg 31800 gcagctttgg agaagataca agcggctctg cctcaaggac catatgctgt gaacctcatt 31860 cattcacctt ttgacagcaa cttggaaaag gggaatgttg atctcttcct cgaaaagggc 31920 gttactgtgg tggaggcttc tgcattcatg accctcactc cccaagttgt tcgttaccgt 31980 gcagccggac tctcaaggaa cgctgatggg agtgtcaaca tcagaaaccg tatcattgga 32040 aaagtgtcca gaacagaact cgccgaaatg ttcatcagac cggctccgga acacctcctt 32100 gagaaactca tagcttctgg tgagataaca caagaacaag ccgaacttgc ccgtcgtgtt 32160 cctgtcgcag acgacatcgc tgttgaagct gattctggtg gtcacactga caaccgtccc 32220 atccacgtca tcttgccttt gatcatcaac cttaggaaca gactgcacag agagtgtggt 32280 tatcctgcgc accttagagt cagagttgga gctggaggcg gtgttggctg cccacaagct 32340 gctgcggctg ccctcacaat gggagctgcc ttcatagtga ctgggactgt caatcaagtt 32400 gctaaacagt ccgggacatg tgacaacgtg agaaaacaac tcagtcaagc cacctacagt 32460 gacatttgca tggctccagc agctgatatg tttgaggaag gtgtgaaact ccaagttctc 32520 aagaagggaa caatgttccc atcaagagct aacaaattgt acgaactctt ttgcaagtac 32580 gactctttcg attcaatgcc tcctgctgaa cttgaaagga tcgagaagag gatcttcaag 32640 agggcacttc aagaggtctg ggaagagacc aaggactttt acatcaatgg tttgaagaat 32700 ccagagaaga tacagagagc ggaacatgac cccaaactca agatgtcttt gtgtttcaga 32760 tggtatcttg gtcttgcatc acgttgggcc aacatgggtg ctcctgatcg tgtcatggac 32820 tatcaagtct ggtgtggtcc agccattgga gccttcaatg acttcatcaa aggaacatat 32880 cttgatcctg ctgtctctaa tgagtaccca tgtgttgtcc agatcaacct tcagattttg 32940 cgtggtgcat gttacctgag gcgtcttaat gctctgagaa atgatccgag gattgatctt 33000 gagactgaag atgctgcctt tgtctatgag ccaacgaatg cgctttgagt agttagctta 33060 atcacttagg tcacctcgag tatcaaaatc tatttagaaa tacacaatat tttgttgcag 33120 gcttgctgga gaatcgatct gctatcataa aaattacaaa aaaattttat ttgcctcaat 33180 tattttagga ttggtattaa ggacgcttaa attatttgtc gggtcactac gcatcattgt 33240 gattgagaag atcagcgata cgaaatattc gtagtactat cgataattta tttgaaaatt 33300 cataagaaaa gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg 33360 cacatttagg atattggccg agattactga atattgagta agatcacgga atttctgaca 33420 ggagcatgtc ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca 33480 ggagcggatc attcattgtt tgtttggttg cctttgccaa catgggagtc caaggttggc 33540 gcgccgaatt cgcggccgct taattaatca cgagtggatg actgtcacga ctgactcatg 33600 actgactact agtgctagcc tcgaggtcga cacaactttg tataataaag ttgtctagtc 33660 tgcttttaca tgcgagacac atcttctaaa gtaattttaa taatagttac tatattcaag 33720 atttcatata tcaaatactc aatattactt ctaaaaaatt aattagatat aattaaaata 33780 ttactttttt aattttaagt ttaattgttg aatttgtgac tattgattta ttattctact 33840 atgtttaaat tgttttatag atagtttaaa gtaaatataa gtaatgtagt agagtgttag 33900 agtgttaccc taaaccataa actataagat ttatggtgga ctaattttca tatatttctt 33960 attgctttta ccttttcttg gtatgtaagt ccgtaactgg aattactgtg ggttgccatg 34020 acactctgtg gtcttttggt tcatgcatgg atcttgcgca agaaaaagac aaagaacaaa 34080 gaaaaaagac aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa ttagtcactg 34140 gctgatcaag atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa cacgtgctta 34200 acatgcactt taaatggctc acccatctca acccacacac aaacacattg cctttttctt 34260 catcatcacc acaaccacct gtatatattc attctcttcc gccacctcaa tttcttcact 34320 tcaacacacg tcaacctgca tatgcgtgtc atcccatgcc caaatctcca tgcatgttcc 34380 aaccaccttc tctcttatat aatacctata aatacctcta atatcactca cttctttcat 34440 catccatcca tccagagtac tactactcta ctactataat accccaaccc aactcatatt 34500 caatactact ctaggatcca caaccaaaaa tgcttcagca cacttggctt ccgaagcctc 34560 ccaatctgac cctcttgtca gatgaggttc atctctggag gattcctctt gaccagcctg 34620 agtcacaact tcaagacctt gctgccaccc tgagcagtga tgaattggcg agggcaaaca 34680 gattctactt tccagaacac agaaggcgtt tcactgctgg gagaggcatc ctcagatcca 34740 tcttgggtgg atacttggga gtggaaccgg gtcaagtcaa gtttgattat gagtcccgtg 34800 ggaaaccgat ccttggtgac agatttgctg agagtggact cctgttcaac ttgtctcaca 34860 gccagaacct tgccttgtgt gctgtcaact acacgcgtca aataggcatt gatcttgaat 34920 atctgcgtcc aacatctgac ttggagtctc ttgcaaagag gttctttctc ccaagagaat 34980 atgaactctt gaggtcactc cctgatgagc agaaacagaa gattttcttt cgttactgga 35040 cttgcaaaga ggcttatctc aaagcaacgg gagatggaat agccaaactt gaagagatcg 35100 agatagcact caccccaaca gaacctgcca agctccaaac agctcctgcg tggtctctgt 35160 tggagcttgt gccagatgac aattgtgttg cagctgtggc tgttgcgggt tttggttggc 35220 agcccaagtt ctggcattac tgagtagtta gcttaatcac ttaggtcacc tcgagtatca 35280 aaatctattt agaaatacac aatattttgt tgcaggcttg ctggagaatc gatctgctat 35340 cataaaaatt acaaaaaaat tttatttgcc tcaattattt taggattggt attaaggacg 35400 cttaaattat ttgtcgggtc actacgcatc attgtgattg agaagatcag cgatacgaaa 35460 tattcgtagt actatcgata atttatttga aaattcataa gaaaagcaaa cgttacatga 35520 attgatgaaa caatacaaag acagataaag ccacgcacat ttaggatatt ggccgagatt 35580 actgaatatt gagtaagatc acggaatttc tgacaggagc atgtcttcaa ttcagcccaa 35640 atggcagttg aaatactcaa accgccccat atgcaggagc ggatcattca ttgtttgttt 35700 ggttgccttt gccaacatgg gagtccaagg ttggcgcgcc gaattcgcgg ccgcttaatt 35760 aactactgtc actgaggccg tagacgagta cggactgatc taactagtgc tagcctcgag 35820 gtcgacaccc agcttt 35836 <210> 50 <211> 47165 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9323 <400> 50 gtacaaaaaa gcaggcttct agacctagct aggtggagtc atcacgcaga ctatctcagc 60 atgtgcgtag cacgcggccg cctcccagta tcattatagt gaaagttttg gctctctcgc 120 cggtggtttt ttacctctat ttaaaggggt tttccaccta aaaattctgg tatcattctc 180 actttacttg ttactttaat ttctcataat ctttggttga aattatcacg cttccgcaca 240 cgatatccct acaaatttat tatttgttaa acattttcaa accgcataaa attttatgaa 300 gtcccgtcta tctttaatgt agtctaacat tttcatattg aaatatataa tttacttaat 360 tttagcgttg gtagaaagca taatgattta ttcttattct tcttcatata aatgtttaat 420 atacaatata aacaaattct ttaccttaag aaggatttcc cattttatat tttaaaaata 480 tatttatcaa atatttttca accacgtaaa tctcataata ataagttgtt tcaaaagtaa 540 taaaatttaa ctccataatt tttttattcg actgatctta aagcaacacc cagtgacaca 600 actagccatt tttttctttg aataaaaaaa tccaattatc attgtatttt ttttatacaa 660 tgaaaatttc accaaacaat gatttgtggt atttctgaag caagtcatgt tatgcaaaat 720 tctataattc ccatttgaca ctacggaagt aactgaagat ctgcttttac atgcgagaca 780 catcttctaa agtaatttta ataatagtta ctatattcaa gatttcatat atcaaatact 840 caatattact tctaaaaaat taattagata taattaaaat attacttttt taattttaag 900 tttaattgtt gaatttgtga ctattgattt attattctac tatgtttaaa ttgttttata 960 gatagtttaa agtaaatata agtaatgtag tagagtgtta gagtgttacc ctaaaccata 1020 aactataaga tttatggtgg actaattttc atatatttct tattgctttt accttttctt 1080 ggtatgtaag tccgtaactg gaattactgt gggttgccat gacactctgt ggtcttttgg 1140 ttcatgcatg gatcttgcgc aagaaaaaga caaagaacaa agaaaaaaga caaaacagag 1200 agacaaaacg caatcacaca accaactcaa attagtcact ggctgatcaa gatcgccgcg 1260 tccatgtatg tctaaatgcc atgcaaagca acacgtgctt aacatgcact ttaaatggct 1320 cacccatctc aacccacaca caaacacatt gcctttttct tcatcatcac cacaaccacc 1380 tgtatatatt cattctcttc cgccacctca atttcttcac ttcaacacac gtcaacctgc 1440 atatgcgtgt catcccatgc ccaaatctcc atgcatgttc caaccacctt ctctcttata 1500 taatacctat aaatacctct aatatcactc acttctttca tcatccatcc atccagagta 1560 ctactactct actactataa taccccaacc caactcatat tcaatactac tctaggatcc 1620 acaaccaaaa atggcggccc gtctgcagga gcaaaaggga ggcgagatgg atacccgcat 1680 tgccatcatc ggcatgtcgg ccatcctccc ctgcggcacg accgtgcgcg agtcgtggga 1740 gaccatccgc gccggcatcg actgcctgtc ggatctcccc gaggaccgcg tcgacgtgac 1800 ggcgtacttt gaccccgtca agaccaccaa ggacaagatc tactgcaagc gcggtggctt 1860 cattcccgag tacgactttg acgcccgcga gttcggactc aacatgttcc agatggagga 1920 ctcggacgca aaccagacca tctcgcttct caaggtcaag gaggccctcc aggacgccgg 1980 catcgacgcc ctcggcaagg aaaagaagaa catcggctgc gtgctcggca ttggcggcgg 2040 ccaaaagtcc agccacgagt tctactcgcg ccttaattat gttgtcgtgg agaaggtcct 2100 ccgcaagatg ggcatgcccg aggaggacgt caaggtcgcc gtcgaaaagt acaaggccaa 2160 cttccccgag tggcgcctcg actccttccc tggcttcctc ggcaacgtca ccgccggtcg 2220 ctgcaccaac accttcaacc tcgacggcat gaactgcgtt gtcgacgccg catgcgcctc 2280 gtccctcatc gccgtcaagg tcgccatcga cgagctgctc tacggtgact gcgacatgat 2340 ggtcaccggt gccacctgca cggataactc catcggcatg tacatggcct tctccaagac 2400 ccccgtgttc tccacggacc ccagcgtgcg cgcctacgac gaaaagacaa agggcatgct 2460 catcggcgag ggctccgcca tgctcgtcct caagcgctac gccgacgccg tccgcgacgg 2520 cgatgagatc cacgctgtta ttcgcggctg cgcctcctcc agtgatggca aggccgccgg 2580 catctacacg cccaccattt cgggccagga ggaggccctc cgccgcgcct acaaccgcgc 2640 ctgtgtcgac ccggccaccg tcactctcgt cgagggtcac ggcaccggta ctcccgttgg 2700 cgaccgcatc gagctcaccg ccttgcgcaa cctctttgac aaggcctacg gcgagggcaa 2760 caccgaaaag gtcgctgtgg gcagcatcaa gtccagcatc ggccatctca aggccgtcgc 2820 cggtctcgcc ggtatgatca aggtcatcat ggcgctcaag cacaagactc tcccgggcac 2880 catcaacgtc gacaacccac ccaacctcta cgacaacacg cccatcaacg agtcctcgct 2940 ctacattaac accatgaacc gcccctggtt cccgccccct ggtgtgcccc gccgcgccgg 3000 catttcgagc tttggctttg gtggcgccaa ctaccacgcc gtcctcgagg aggccgagcc 3060 cgagcacacg accgcgtacc gcctcaacaa gcgcccgcag cccgtgctca tgatggccgc 3120 cacgcccgcg gccctccagt cgctctgcga ggcccagctc aaggagttcg aggccgccat 3180 caaggagaac gagaccgtca agaacaccgc ctacatcaag tgcgtcaagt tcggcgagca 3240 gttcaaattc cctggctcca tcccggccac aaacgcgcgc ctcggcttcc tcgtcaagga 3300 tgctgaggat gcctgctcca ccctccgtgc catctgcgcc caattcgcca aggatgtcac 3360 caaggaggcc tggcgcctcc cccgcgaggg cgtcagcttc cgcgccaagg gcatcgccac 3420 caacggcgct gtcgccgcgc tcttctccgg ccagggcgcg cagtacacgc acatgtttag 3480 cgaggtggcc atgaactggc cccagttccg ccagagcatt gccgccatgg acgccgccca 3540 gtccaaggtc gctggaagcg acaaggactt tgagcgcgtc tcccaggtcc tctacccgcg 3600 caagccgtac gagcgtgagc ccgagcagga ccacaagaag atctccctca ccgcctactc 3660 gcagccctcg accctggcct gcgctctcgg tgcctttgag atcttcaagg aggccggctt 3720 caccccggac tttgccgccg gccattcgct cggtgagttc gccgccctct acgccgcggg 3780 ctgcgtcgac cgcgacgagc tctttgagct tgtctgccgc cgcgcccgca tcatgggcgg 3840 caaggacgca ccggccaccc ccaagggctg catggccgcc gtcattggcc ccaacgccga 3900 gaacatcaag gtccaggccg ccaacgtctg gctcggcaac tccaactcgc cttcgcagac 3960 cgtcatcacc ggctccgtcg aaggtatcca ggccgagagc gcccgcctcc agaaggaggg 4020 cttccgcgtc gtgcctcttg cctgcgagag cgccttccac tcgccccaga tggagaacgc 4080 ctcgtcggcc ttcaaggacg tcatctccaa ggtctccttc cgcaccccca aggccgagac 4140 caagctcttc agcaacgtct ctggcgagac ctaccccacg gacgcccgcg agatgcttac 4200 gcagcacatg accagcagcg tcaagttcct cacccaggtc cgcaacatgc accaggccgg 4260 tgcgcgcatc tttgtcgagt tcggacccaa gcaggtgctc tccaagcttg tctccgagac 4320 cctcaaggat gacccctcgg ttgtcaccgt ctctgtcaac ccggcctcgg gcacggattc 4380 ggacatccag ctccgcgacg cggccgtcca gctcgttgtc gctggcgtca accttcaggg 4440 ctttgacaag tgggacgccc ccgatgccac ccgcatgcag gccatcaaga agaagcgcac 4500 taccctccgc ctttcggccg ccacctacgt ctcggacaag accaagaagg tccgcgacgc 4560 cgccatgaac gatggccgct gcgtcaccta cctcaagggc gccgcaccgc tcatcaaggc 4620 cccggagccc gttgtcgacg aggccgccaa gcgcgaggcc gagcgtctcc agaaggagct 4680 tcaggatgcc cagcgccagc tcgacgacgc caagcgcgcc gccgccgagg ccaactccaa 4740 gctcgccgct gccaaggagg aggccaagac cgccgctgct tcggccaagc ccgcagttga 4800 cactgctgtt gtcgaaaagc atcgtgccat cctcaagtcc atgctcgcgg agctcgatgg 4860 ctacggatcg gtcgacgctt cttccctcca gcagcagcag cagcagcaga cggcccccgc 4920 cccggtcaag gctgctgcgc ctgccgcccc cgttgcctcg gcccctgccc cggctgtctc 4980 gaacgagctt cttgagaagg ccgagactgt cgtcatggag gtcctcgccg ccaagaccgg 5040 ctacgagacc gacatgatcg aggctgacat ggagctcgag accgagctcg gcattgactc 5100 catcaagcgt gtcgagatcc tctccgaggt ccaggccatg ctcaatgtcg aggccaagga 5160 tgtcgatgcc ctcagccgca ctcgcactgt tggtgaggtt gtcaacgcca tgaaggccga 5220 gatcgctggc agctctgccc cggcgcctgc tgccgctgct ccggctccgg ccaaggctgc 5280 ccctgccgcc gctgcgcctg ctgtctcgaa cgagcttctc gagaaggccg agaccgtcgt 5340 catggaggtc ctcgccgcca agactggcta cgagactgac atgatcgagt ccgacatgga 5400 gctcgagact gagctcggca ttgactccat caagcgtgtc gagatcctct ccgaggttca 5460 ggccatgctc aacgtcgagg ccaaggacgt cgacgctctc agccgcactc gcactgtggg 5520 tgaggtcgtc aacgccatga aggctgagat cgctggtggc tctgccccgg cgcctgccgc 5580 cgctgcccca ggtccggctg ctgccgcccc tgcgcctgcc gccgccgccc ctgctgtctc 5640 gaacgagctt cttgagaagg ccgagaccgt cgtcatggag gtcctcgccg ccaagactgg 5700 ctacgagact gacatgatcg agtccgacat ggagctcgag accgagctcg gcattgactc 5760 catcaagcgt gtcgagattc tctccgaggt ccaggccatg ctcaacgtcg aggccaagga 5820 cgtcgacgct ctcagccgca cccgcactgt tggcgaggtc gtcgatgcca tgaaggccga 5880 gatcgctggt ggctctgccc cggcgcctgc cgccgctgct cctgctccgg ctgctgccgc 5940 ccctgcgcct gccgcccctg cgcctgctgt ctcgagcgag cttctcgaga aggccgagac 6000 tgtcgtcatg gaggtcctcg ccgccaagac tggctacgag actgacatga tcgagtccga 6060 catggagctc gagaccgagc tcggcattga ctccatcaag cgtgtcgaga ttctctccga 6120 ggtccaggcc atgctcaacg tcgaggccaa ggacgtcgac gctctcagcc gcacccgcac 6180 tgttggcgag gtcgtcgatg ccatgaaggc cgagatcgct ggtggctctg ccccggcgcc 6240 tgccgccgct gctcctgctc cggctgctgc cgcccctgcg cctgccgccc ctgcgcctgc 6300 cgcccctgcg cctgctgtct cgagcgagct tctcgagaag gccgagactg tcgtcatgga 6360 ggtcctcgcc gccaagactg gctacgagac tgacatgatt gagtccgaca tggagctcga 6420 gaccgagctc ggcattgact ccatcaagcg tgtcgagatt ctctccgagg ttcaggccat 6480 gctcaacgtc gaggccaagg acgtcgacgc tctcagccgc actcgcactg ttggtgaggt 6540 cgtcgatgcc atgaaggctg agatcgctgg cagctccgcc tcggcgcctg ccgccgctgc 6600 tcctgctccg gctgctgccg ctcctgcgcc cgctgccgcc gcccctgctg tctcgaacga 6660 gcttctcgag aaagccgaga ctgtcgtcat ggaggtcctc gccgccaaga ctggctacga 6720 gactgacatg atcgagtccg acatggagct cgagactgag ctcggcattg actccatcaa 6780 gcgtgtcgag atcctctccg aggttcaggc catgctcaac gtcgaggcca aggacgtcga 6840 tgccctcagc cgcacccgca ctgttggcga ggttgtcgat gccatgaagg ccgagatcgc 6900 tggtggctct gccccggcgc ctgccgccgc tgcccctgct ccggctgccg ccgcccctgc 6960 tgtctcgaac gagcttctcg agaaggccga gactgtcgtc atggaggtcc tcgccgccaa 7020 gactggctac gagaccgaca tgatcgagtc cgacatggag ctcgagaccg agctcggcat 7080 tgactccatc aagcgtgtcg agattctctc cgaggttcag gccatgctca acgtcgaggc 7140 caaggacgtc gatgctctca gccgcactcg cactgttggc gaggtcgtcg atgccatgaa 7200 ggctgagatc gccggcagct ccgccccggc gcctgccgcc gctgctcctg ctccggctgc 7260 tgccgctcct gcgcccgctg ccgctgcccc tgctgtctcg agcgagcttc tcgagaaggc 7320 cgagaccgtc gtcatggagg tcctcgccgc caagactggc tacgagactg acatgattga 7380 gtccgacatg gagctcgaga ctgagctcgg cattgactcc atcaagcgtg tcgagatcct 7440 ctccgaggtt caggccatgc tcaacgtcga ggccaaggac gtcgatgccc tcagccgcac 7500 ccgcactgtt ggcgaggttg tcgatgccat gaaggccgag atcgctggtg gctctgcccc 7560 ggcgcctgcc gccgctgccc ctgctccggc tgccgccgcc cctgctgtct cgaacgagct 7620 tcttgagaag gccgagaccg tcgtcatgga ggtcctcgcc gccaagactg gctacgagac 7680 cgacatgatc gagtccgaca tggagctcga gaccgagctc ggcattgact ccatcaagcg 7740 tgtcgagatt ctctccgagg ttcaggccat gctcaacgtc gaggccaagg acgtcgacgc 7800 tctcagccgc actcgcactg ttggcgaggt cgtcgatgcc atgaaggctg agatcgctgg 7860 tggctctgcc ccggcgcctg ccgccgctgc tcctgcctcg gctggcgccg cgcctgcggt 7920 caagattgac tcggtccacg gcgctgactg tgatgatctt tccctgatgc acgccaaggt 7980 ggttgacatc cgccgcccgg acgagctcat cctggagcgc cccgagaacc gccccgttct 8040 cgttgtcgat gacggcagcg agctcaccct cgccctggtc cgcgtcctcg gcgcctgcgc 8100 cgttgtcctg acctttgagg gtctccagct cgctcagcgc gctggtgccg ctgccatccg 8160 ccacgtgctc gccaaggatc tttccgcgga gagcgccgag aaggccatca aggaggccga 8220 gcagcgcttt ggcgctctcg gcggcttcat ctcgcagcag gcggagcgct tcgagcccgc 8280 cgaaatcctc ggcttcacgc tcatgtgcgc caagttcgcc aaggcttccc tctgcacggc 8340 tgtggctggc ggccgcccgg cctttatcgg tgtggcgcgc cttgacggcc gcctcggatt 8400 cacttcgcag ggcacttctg acgcgctcaa gcgtgcccag cgtggtgcca tctttggcct 8460 ctgcaagacc atcggcctcg agtggtccga gtctgacgtc ttttcccgcg gcgtggacat 8520 tgctcagggc atgcaccccg aggatgccgc cgtggcgatt gtgcgcgaga tggcgtgcgc 8580 tgacattcgc attcgcgagg tcggcattgg cgcaaaccag cagcgctgca cgatccgtgc 8640 cgccaagctc gagaccggca acccgcagcg ccagatcgcc aaggacgacg tgctgctcgt 8700 ttctggcggc gctcgcggca tcacgcctct ttgcatccgg gagatcacgc gccagatcgc 8760 gggcggcaag tacattctgc ttggccgcag caaggtctct gcgagcgaac cggcatggtg 8820 cgctggcatc actgacgaga aggctgtgca aaaggctgct acccaggagc tcaagcgcgc 8880 ctttagcgct ggcgagggcc ccaagcccac gccccgcgct gtcactaagc ttgtgggctc 8940 tgttcttggc gctcgcgagg tgcgcagctc tattgctgcg attgaagcgc tcggcggcaa 9000 ggccatctac tcgtcgtgcg acgtgaactc tgccgccgac gtggccaagg ccgtgcgcga 9060 tgccgagtcc cagctcggtg cccgcgtctc gggcatcgtt catgcctcgg gcgtgctccg 9120 cgaccgtctc atcgagaaga agctccccga cgagttcgac gccgtctttg gcaccaaggt 9180 caccggtctc gagaacctcc tcgccgccgt cgaccgcgcc aacctcaagc acatggtcct 9240 cttcagctcg ctcgccggct tccacggcaa cgtcggccag tctgactacg ccatggccaa 9300 cgaggccctt aacaagatgg gcctcgagct cgccaaggac gtctcggtca agtcgatctg 9360 cttcggtccc tgggacggtg gcatggtgac gccgcagctc aagaagcagt tccaggagat 9420 gggcgtgcag atcatccccc gcgagggcgg cgctgatacc gtggcgcgca tcgtgctcgg 9480 ctcctcgccg gctgagatcc ttgtcggcaa ctggcgcacc ccgtccaaga aggtcggctc 9540 ggacaccatc accctgcacc gcaagatttc cgccaagtcc aaccccttcc tcgaggacca 9600 cgtcatccag ggccgccgcg tgctgcccat gacgctggcc attggctcgc tcgcggagac 9660 ctgcctcggc ctcttccccg gctactcgct ctgggccatt gacgacgccc agctcttcaa 9720 gggtgtcact gtcgacggcg acgtcaactg cgaggtgacc ctcaccccgt cgacggcgcc 9780 ctcgggccgc gtcaacgtcc aggccacgct caagaccttt tccagcggca agctggtccc 9840 ggcctaccgc gccgtcatcg tgctctccaa ccagggcgcg cccccggcca acgccaccat 9900 gcagccgccc tcgctcgatg ccgatccggc gctccagggc tccgtctacg acggcaagac 9960 cctcttccac ggcccggcct tccgcggcat cgatgacgtg ctctcgtgca ccaagagcca 10020 gcttgtggcc aagtgcagcg ctgtccccgg ctccgacgcc gctcgcggcg agtttgccac 10080 ggacactgac gcccatgacc ccttcgtgaa cgacctggcc tttcaggcca tgctcgtctg 10140 ggtgcgccgc acgctcggcc aggctgcgct ccccaactcg atccagcgca tcgtccagca 10200 ccgcccggtc ccgcaggaca agcccttcta cattaccctc cgctccaacc agtcgggcgg 10260 tcactcccag cacaagcacg cccttcagtt ccacaacgag cagggcgatc tcttcattga 10320 tgtccaggct tcggtcatcg ccacggacag ccttgccttc taaatctaaa ctaggccatg 10380 gtgacttaag gtagttagct taatcactta ggtcaccagt atgaactaaa atgcatgtag 10440 gtgtaagagc tcatggagag catggaatat tgtatccgac catgtaacag tataataact 10500 gagctccatc tcacttcttc tatgaataaa caaaggatgt tatgatatat taacactcta 10560 tctatgcacc ttattgttct atgataaatt tcctcttatt attataaatc atctgaatcg 10620 tgacggctta tggaatgctt caaatagtac aaaaacaaat gtgtactata agactttcta 10680 aacaattcta actttagcat tgtgaacgag acataagtgt taagaagaca taacaattat 10740 aatggaagaa gtttgtctcc atttatatat tatatattac ccacttatgt attatattag 10800 gatgttaagg agacataaca attataaaga gagaagtttg tatccattta tatattatat 10860 actacccatt tatatattat acttatccac ttatttaatg tctttataag gtttgatcca 10920 tgatatttct aatattttag ttgatatgta tatgaaaagg tactatttga actctcttac 10980 tctgtataaa ggttggatca tccttaaagt gggtctattt aattttattg cttcttacag 11040 ataaaaaaaa aattatgagt tggtttgata aaatattgaa ggatttaaaa taataataaa 11100 taataaataa catataatat atgtatataa atttattata atataacatt tatctataaa 11160 aaagtaaata ttgtcataaa tctatacaat cgtttagcct tgctggaacg aatctcaatt 11220 atttaaacga gagtaaacat atttgacttt ttggttattt aacaaattat tatttaacac 11280 tatatgaaat tttttttttt tatcagcaaa gaataaaatt aaattaagaa ggacaatggt 11340 gtcccaatcc ttatacaacc aacttccaca agaaagtcaa gtcagagaca acaaaaaaac 11400 aagcaaagga aattttttaa tttgagttgt cttgtttgct gcataattta tgcagtaaaa 11460 cactacacat aaccctttta gcagtagagc aatggttgac cgtgtgctta gcttctttta 11520 ttttattttt ttatcagcaa agaataaata aaataaaatg agacacttca gggatgtttc 11580 aacccttata caaaacccca aaaacaagtt tcctagcacc ctaccaacga attcgcggcc 11640 gcttaattaa gatgagtgat actcaggact caggactcac tctgctgatc actagtgcta 11700 gcctcgaggt cgacacaact ttgtatacaa aagttgtcta gacctaggct cagcaactca 11760 tccagtcaag ttcgagtcag tgagttgggc ggccgcctcc cagtatcatt atagtgaaag 11820 ttttggctct ctcgccggtg gttttttacc tctatttaaa ggggttttcc acctaaaaat 11880 tctggtatca ttctcacttt acttgttact ttaatttctc ataatctttg gttgaaatta 11940 tcacgcttcc gcacacgata tccctacaaa tttattattt gttaaacatt ttcaaaccgc 12000 ataaaatttt atgaagtccc gtctatcttt aatgtagtct aacattttca tattgaaata 12060 tataatttac ttaattttag cgttggtaga aagcataatg atttattctt attcttcttc 12120 atataaatgt ttaatataca atataaacaa attctttacc ttaagaagga tttcccattt 12180 tatattttaa aaatatattt atcaaatatt tttcaaccac gtaaatctca taataataag 12240 ttgtttcaaa agtaataaaa tttaactcca taattttttt attcgactga tcttaaagca 12300 acacccagtg acacaactag ccattttttt ctttgaataa aaaaatccaa ttatcattgt 12360 atttttttta tacaatgaaa atttcaccaa acaatgattt gtggtatttc tgaagcaagt 12420 catgttatgc aaaattctat aattcccatt tgacactacg gaagtaactg aagatctgct 12480 tttacatgcg agacacatct tctaaagtaa ttttaataat agttactata ttcaagattt 12540 catatatcaa atactcaata ttacttctaa aaaattaatt agatataatt aaaatattac 12600 ttttttaatt ttaagtttaa ttgttgaatt tgtgactatt gatttattat tctactatgt 12660 ttaaattgtt ttatagatag tttaaagtaa atataagtaa tgtagtagag tgttagagtg 12720 ttaccctaaa ccataaacta taagatttat ggtggactaa ttttcatata tttcttattg 12780 cttttacctt ttcttggtat gtaagtccgt aactggaatt actgtgggtt gccatgacac 12840 tctgtggtct tttggttcat gcatggatct tgcgcaagaa aaagacaaag aacaaagaaa 12900 aaagacaaaa cagagagaca aaacgcaatc acacaaccaa ctcaaattag tcactggctg 12960 atcaagatcg ccgcgtccat gtatgtctaa atgccatgca aagcaacacg tgcttaacat 13020 gcactttaaa tggctcaccc atctcaaccc acacacaaac acattgcctt tttcttcatc 13080 atcaccacaa ccacctgtat atattcattc tcttccgcca cctcaatttc ttcacttcaa 13140 cacacgtcaa cctgcatatg cgtgtcatcc catgcccaaa tctccatgca tgttccaacc 13200 accttctctc ttatataata cctataaata cctctaatat cactcacttc tttcatcatc 13260 catccatcca gagtactact actctactac tataataccc caacccaact catattcaat 13320 actactctag gatccggcca tctaggccgg atccacaacc aaaaatggcc gctcgcaacg 13380 tgtctgcagc gcatgagatg cacgatgaaa agcgcatcgc cgtcgtcggc atggccgtcc 13440 agtacgccgg atgcaaaacc aaggacgagt tctgggaggt gctcatgaac ggcaaggtcg 13500 agtccaaggt gatcagcgac aaacgactcg gctccaacta ccgcgccgag cactacaaag 13560 cagagcgcag caagtatgcc gacacctttt gcaacgaaac gtacggcacc cttgacgaga 13620 acgagatcga caacgagcac gaactcctcc tcaacctcgc caagcaggca ctcgcagaga 13680 catccgtcaa agactcgaca cgctgcggca tcgtcagcgg ctgcctctcg ttccccatgg 13740 acaacctcca gggtgaactc ctcaacgtgt accaaaacca tgtcgagaaa aagctcgggg 13800 cccgcgtctt caaggacgcc tcccattggt ccgaacgcga gcagtccaac aaacccgagg 13860 ccggtgaccg ccgcatcttc atggacccgg cctccttcgt cgccgaagaa ctcaacctcg 13920 gcgcccttca ctactccgtc gacgcagcat gcgccacggc gctctacgtg ctccgcctcg 13980 cgcaggatca tctcgtctcc ggcgccgccg acgtcatgct ctgcggtgcc acctgcctgc 14040 cggagccctt tttcatcctt tcgggctttt ccaccttcca ggccatgccc gtcggcacgg 14100 gccagaacgt gtccatgccg ctgcacaagg acagccaggg cctcaccccg ggtgagggcg 14160 gctccatcat ggtcctcaag cgtctcgatg atgccatccg cgacggcgac cacatctacg 14220 gcacccttct cggcgccaat gtcagcaact ccggcacagg tctgcccctc aagccccttc 14280 tccccagcga gaaaaagtgc ctcatggaca cctacacgcg cattaacgtg cacccgcaca 14340 agattcagta cgtcgagtgc cacgccaccg gcacgcccca gggtgatcgt gtggaaatcg 14400 acgccgtcaa ggcctgcttt gaaggcaagg tcccccgttt cggtaccaca aagggcaact 14460 ttggacacac cctcgtcgca gccggctttg ccggtatgtg caaggtcctc ctctccatga 14520 agcatggcat catcccgccc accccgggta tcgatgacga gaccaagatg gaccctctcg 14580 tcgtctccgg tgaggccatc ccatggccag agaccaacgg cgagcccaag cgcgccggtc 14640 tctcggcctt tggctttggt ggcaccaacg cccatgccgt ctttgaggag catgacccct 14700 ccaacgccgc ctgcacgggc cacgactcca tttctgcgct ctcggcccgc tgcggcggtg 14760 aaagcaacat gcgcatcgcc atcactggta tggacgccac ctttggcgct ctcaagggac 14820 tcgacgcctt cgagcgcgcc atttacaccg gcgctcacgg tgccatccca ctcccagaaa 14880 agcgctggcg ctttctcggc aaggacaagg actttcttga cctctgcggc gtcaaggcca 14940 ccccgcacgg ctgctacatt gaagatgttg aggtcgactt ccagcgcctc cgcacgccca 15000 tgacccctga agacatgctc ctccctcagc agcttctggc cgtcaccacc attgaccgcg 15060 ccatcctcga ctcgggaatg aaaaagggtg gcaatgtcgc cgtctttgtc ggcctcggca 15120 ccgacctcga gctctaccgt caccgtgctc gcgtcgctct caaggagcgc gtccgccctg 15180 aagcctccaa gaagctcaat gacatgatgc agtacattaa cgactgcggc acatccacat 15240 cgtacacctc gtacattggc aacctcgtcg ccacgcgcgt ctcgtcgcag tggggcttca 15300 cgggcccctc ctttacgatc accgagggca acaactccgt ctaccgctgc gccgagctcg 15360 gcaagtacct cctcgagacc ggcgaggtcg atggcgtcgt cgttgcgggt gtcgatctct 15420 gcggcagtgc cgaaaacctt tacgtcaagt ctcgccgctt caaggtgtcc acctccgata 15480 ccccgcgcgc cagctttgac gccgccgccg atggctactt tgtcggcgag ggctgcggtg 15540 cctttgtgct caagcgtgag actagctgca ccaaggacga ccgtatctac gcttgcatgg 15600 atgccatcgt ccctggcaac gtccctagcg cctgcttgcg cgaggccctc gaccaggcgc 15660 gcgtcaagcc gggcgatatc gagatgctcg agctcagcgc cgactccgcc cgccacctca 15720 aggacccgtc cgtcctgccc aaggagctca ctgccgagga ggaaatcggc ggccttcaga 15780 cgatccttcg tgacgatgac aagctcccgc gcaacgtcgc aacgggcagt gtcaaggcca 15840 ccgtcggtga caccggttat gcctctggtg ctgccagcct catcaaggct gcgctttgca 15900 tctacaaccg ctacctgccc agcaacggcg acgactggga tgaacccgcc cctgaggcgc 15960 cctgggacag caccctcttt gcgtgccaga cctcgcgcgc ttggctcaag aaccctggcg 16020 agcgtcgcta tgcggccgtc tcgggcgtct ccgagacgcg ctcgtgctat tccgtgctcc 16080 tctccgaagc cgagggccac tacgagcgcg agaaccgcat ctcgctcgac gaggaggcgc 16140 ccaagctcat tgtgcttcgc gccgactccc acgaggagat ccttggtcgc ctcgacaaga 16200 tccgcgagcg cttcttgcag cccacgggcg ccgccccgcg cgagtccgag ctcaaggcgc 16260 aggcccgccg catcttcctc gagctcctcg gcgagaccct tgcccaggat gccgcttctt 16320 caggctcgca aaagcccctc gctctcagcc tcgtctccac gccctccaag ctccagcgcg 16380 aggtcgagct cgcggccaag ggtatcccgc gctgcctcaa gatgcgccgc gattggagct 16440 cccctgctgg cagccgctac gcgcctgagc cgctcgccag cgaccgcgtc gccttcatgt 16500 acggcgaagg tcgcagccct tactacggca tcacccaaga cattcaccgc atttggcccg 16560 aactccacga ggtcatcaac gaaaagacga accgtctctg ggccgaaggc gaccgctggg 16620 tcatgccgcg cgccagcttc aagtcggagc tcgagagcca gcagcaagag tttgatcgca 16680 acatgattga aatgttccgt cttggaatcc tcacctcaat tgccttcacc aatctggcgc 16740 gcgacgttct caacatcacg cccaaggccg cctttggcct cagtcttggc gagatttcca 16800 tgatttttgc cttttccaag aagaacggtc tcatctccga ccagctcacc aaggatcttc 16860 gcgagtccga cgtgtggaac aaggctctgg ccgttgaatt taatgcgctg cgcgaggcct 16920 ggggcattcc acagagtgtc cccaaggacg agttctggca aggctacatt gtgcgcggca 16980 ccaagcagga tatcgaggcg gccatcgccc cggacagcaa gtacgtgcgc ctcaccatca 17040 tcaatgatgc caacaccgcc ctcattagcg gcaagcccga cgcctgcaag gctgcgatcg 17100 cgcgtctcgg tggcaacatt cctgcgcttc ccgtgaccca gggcatgtgc ggccactgcc 17160 ccgaggtggg accttatacc aaggatatcg ccaagatcca tgccaacctt gagttccccg 17220 ttgtcgacgg ccttgacctc tggaccacaa tcaaccagaa gcgcctcgtg ccacgcgcca 17280 cgggcgccaa ggacgaatgg gccccttctt cctttggcga gtacgccggc cagctctacg 17340 agaagcaggc taacttcccc caaatcgtcg agaccattta caagcaaaac tacgacgtct 17400 ttgtcgaggt tgggcccaac aaccaccgta gcaccgcagt gcgcaccacg cttggtcccc 17460 agcgcaacca ccttgctggc gccatcgaca agcagaacga ggatgcttgg acgaccatcg 17520 tcaagcttgt ggcttcgctc aaggcccacc ttgttcctgg cgtcacgatc tcgccgctgt 17580 accactccaa gcttgtggcg gaggctgagg cttgctacgc tgcgctctgc aagggtgaaa 17640 agcccaagaa gaacaagttt gtgcgcaaga ttcagctcaa cggtcgcttc aacagcaagg 17700 cggaccccat ctcctcggcc gatcttgcca gctttccgcc tgcggaccct gccattgaag 17760 ccgccatctc gagccgcatc atgaagccgg ttgctccgaa gttctacgcg cgtctcaaca 17820 ttgacgagca ggacgagacc cgtgatccga tcctcaacaa ggacaacgcg ccgtcttcca 17880 gctctagctc ctcttccagc tcttccagct cttccagccc gtcgccagct ccgtccgccc 17940 cagtgcaaaa gaaggctgct ccggccgcgg agaccaaggc tgttgcttcg gctgacgcac 18000 ttcgcagtgc cctgctcgat ctcgacagta tgcttgcgct gagctctgcc agtgcctccg 18060 gcaaccttgt tgagactgcg cctagcgacg cctcggtcat tgtgccgccc tgcaacattg 18120 cggatctcgg cagccgcgcc ttcatgaaaa cgtacggtgt ttcggcgcct ctgtacacgg 18180 gcgccatggc caagggcatt gcctctgcgg acctcgtcat tgccgccggc cgccagggca 18240 tccttgcgtc ctttggcgcc ggcggacttc ccatgcaggt tgtgcgtgag tccatcgaaa 18300 agattcaggc cgccctgccc aatggcccgt acgctgtcaa ccttatccat tctccctttg 18360 acagcaacct cgaaaagggc aatgtcgatc tcttcctcga gaagggtgtc acctttgtcg 18420 aggcctcggc ctttatgacg ctcaccccgc aggtcgtgcg gtaccgcgcg gctggcctca 18480 cgcgcaacgc cgacggctcg gtcaacatcc gcaaccgtat cattggcaag gtctcgcgca 18540 ccgagctcgc cgagatgttc atgcgtcctg cgcccgagca ccttcttcag aagctcattg 18600 cttccggcga gatcaaccag gagcaggccg agctcgcccg ccgtgttccc gtcgctgacg 18660 acatcgcggt cgaagctgac tcgggtggcc acaccgacaa ccgccccatc cacgtcattc 18720 tgcccctcat catcaacctt cgcgaccgcc ttcaccgcga gtgcggctac ccggccaacc 18780 ttcgcgtccg tgtgggcgcc ggcggtggca ttgggtgccc ccaggcggcg ctggccacct 18840 tcaacatggg tgcctccttt attgtcaccg gcaccgtgaa ccaggtcgcc aagcagtcgg 18900 gcacgtgcga caatgtgcgc aagcagctcg cgaaggccac ttactcggac gtatgcatgg 18960 ccccggctgc cgacatgttc gaggaaggcg tcaagcttca ggtcctcaag aagggaacca 19020 tgtttccctc gcgcgccaac aagctctacg agctcttttg caagtacgac tcgttcgagt 19080 ccatgccccc cgcagagctt gcgcgcgtcg agaagcgcat cttcagccgc gcgctcgaag 19140 aggtctggga cgagaccaaa aacttttaca ttaaccgtct tcacaacccg gagaagatcc 19200 agcgcgccga gcgcgacccc aagctcaaga tgtcgctgtg ctttcgctgg tacctgagcc 19260 tggcgagccg ctgggccaac actggagctt ccgatcgcgt catggactac caggtctggt 19320 gcggtcctgc cattggttcc ttcaacgatt tcatcaaggg aacttacctt gatccggccg 19380 tcgcaaacga gtacccgtgc gtcgttcaga ttaacaagca gatccttcgt ggagcgtgct 19440 tcttgcgccg tctcgaaatt ctgcgcaacg cacgcctttc cgatggcgct gccgctcttg 19500 tggccagcat cgatgacaca tacgtcccgg ccgagaagct gtgagtagtt agcttaatca 19560 cttaggtcac cggcctagta ggccggtcac cagtatgaac taaaatgcat gtaggtgtaa 19620 gagctcatgg agagcatgga atattgtatc cgaccatgta acagtataat aactgagctc 19680 catctcactt cttctatgaa taaacaaagg atgttatgat atattaacac tctatctatg 19740 caccttattg ttctatgata aatttcctct tattattata aatcatctga atcgtgacgg 19800 cttatggaat gcttcaaata gtacaaaaac aaatgtgtac tataagactt tctaaacaat 19860 tctaacttta gcattgtgaa cgagacataa gtgttaagaa gacataacaa ttataatgga 19920 agaagtttgt ctccatttat atattatata ttacccactt atgtattata ttaggatgtt 19980 aaggagacat aacaattata aagagagaag tttgtatcca tttatatatt atatactacc 20040 catttatata ttatacttat ccacttattt aatgtcttta taaggtttga tccatgatat 20100 ttctaatatt ttagttgata tgtatatgaa aaggtactat ttgaactctc ttactctgta 20160 taaaggttgg atcatcctta aagtgggtct atttaatttt attgcttctt acagataaaa 20220 aaaaaattat gagttggttt gataaaatat tgaaggattt aaaataataa taaataataa 20280 ataacatata atatatgtat ataaatttat tataatataa catttatcta taaaaaagta 20340 aatattgtca taaatctata caatcgttta gccttgctgg aacgaatctc aattatttaa 20400 acgagagtaa acatatttga ctttttggtt atttaacaaa ttattattta acactatatg 20460 aaattttttt tttttatcag caaagaataa aattaaatta agaaggacaa tggtgtccca 20520 atccttatac aaccaacttc cacaagaaag tcaagtcaga gacaacaaaa aaacaagcaa 20580 aggaaatttt ttaatttgag ttgtcttgtt tgctgcataa tttatgcagt aaaacactac 20640 acataaccct tttagcagta gagcaatggt tgaccgtgtg cttagcttct tttattttat 20700 ttttttatca gcaaagaata aataaaataa aatgagacac ttcagggatg tttcaaccct 20760 tatacaaaac cccaaaaaca agtttcctag caccctacca acgaattcgc ggccgcttaa 20820 ttaatctgac ctgaccacta gacctgagtg agtacagatg tgctactagt gctagcctcg 20880 aggtcgacca acttttctat acaaagttgt ctagacctag gactaggcac tgagtgactc 20940 actgacctga ctgagtgact ggcggccgcc tcccagtatc attatagtga aagttttggc 21000 tctctcgccg gtggtttttt acctctattt aaaggggttt tccacctaaa aattctggta 21060 tcattctcac tttacttgtt actttaattt ctcataatct ttggttgaaa ttatcacgct 21120 tccgcacacg atatccctac aaatttatta tttgttaaac attttcaaac cgcataaaat 21180 tttatgaagt cccgtctatc tttaatgtag tctaacattt tcatattgaa atatataatt 21240 tacttaattt tagcgttggt agaaagcata atgatttatt cttattcttc ttcatataaa 21300 tgtttaatat acaatataaa caaattcttt accttaagaa ggatttccca ttttatattt 21360 taaaaatata tttatcaaat atttttcaac cacgtaaatc tcataataat aagttgtttc 21420 aaaagtaata aaatttaact ccataatttt tttattcgac tgatcttaaa gcaacaccca 21480 gtgacacaac tagccatttt tttctttgaa taaaaaaatc caattatcat tgtatttttt 21540 ttatacaatg aaaatttcac caaacaatga tttgtggtat ttctgaagca agtcatgtta 21600 tgcaaaattc tataattccc atttgacact acggaagtaa ctgaagatct gcttttacat 21660 gcgagacaca tcttctaaag taattttaat aatagttact atattcaaga tttcatatat 21720 caaatactca atattacttc taaaaaatta attagatata attaaaatat tactttttta 21780 attttaagtt taattgttga atttgtgact attgatttat tattctacta tgtttaaatt 21840 gttttataga tagtttaaag taaatataag taatgtagta gagtgttaga gtgttaccct 21900 aaaccataaa ctataagatt tatggtggac taattttcat atatttctta ttgcttttac 21960 cttttcttgg tatgtaagtc cgtaactgga attactgtgg gttgccatga cactctgtgg 22020 tcttttggtt catgcatgga tcttgcgcaa gaaaaagaca aagaacaaag aaaaaagaca 22080 aaacagagag acaaaacgca atcacacaac caactcaaat tagtcactgg ctgatcaaga 22140 tcgccgcgtc catgtatgtc taaatgccat gcaaagcaac acgtgcttaa catgcacttt 22200 aaatggctca cccatctcaa cccacacaca aacacattgc ctttttcttc atcatcacca 22260 caaccacctg tatatattca ttctcttccg ccacctcaat ttcttcactt caacacacgt 22320 caacctgcat atgcgtgtca tcccatgccc aaatctccat gcatgttcca accaccttct 22380 ctcttatata atacctataa atacctctaa tatcactcac ttctttcatc atccatccat 22440 ccagagtact actactctac tactataata ccccaaccca actcatattc aatactactc 22500 taggatccgg ccatctaggc cggatccaca accaaaaatg gcgctccgtg tcaagacgaa 22560 caagaagcca tgctgggaga tgaccaagga ggagctgacc agcggcaaga ccgaggtgtt 22620 caactatgag gaactcctcg agttcgcaga gggcgacatc gccaaggtct tcggacccga 22680 gttcgccgtc atcgacaagt acccgcgccg cgtgcgcctg cccgcccgcg agtacctgct 22740 cgtgacccgc gtcaccctca tggacgccga ggtcaacaac taccgcgtcg gcgcccgcat 22800 ggtcaccgag tacgatctcc ccgtcaacgg agagctctcc gagggcggag actgcccctg 22860 ggccgtcctg gtcgagagtg gccagtgcga tctcatgctc atctcctaca tgggcattga 22920 cttccagaac cagggcgacc gcgtctaccg cctgctcaac accacgctca ccttttacgg 22980 cgtggcccac gagggcgaga ccctcgagta cgacattcgc gtcaccggct tcgccaagcg 23040 tctcgacggc ggcatctcca tgttcttctt cgagtacgac tgctacgtca acggccgcct 23100 cctcatcgag atgcgcgatg gctgcgccgg cttcttcacc aacgaggagc tcgacgccgg 23160 caagggcgtc gtcttcaccc gcggcgacct cgccgcccgc gccaagatcc caaagcagga 23220 cgtctccccc tacgccgtcg ccccctgcct ccacaagacc aagctcaacg aaaaggagat 23280 gcagaccctc gtcgacaagg actgggcatc cgtctttggc tccaagaacg gcatgccgga 23340 aatcaactac aaactctgcg cgcgtaagat gctcatgatt gaccgcgtca ccagcattga 23400 ccacaagggc ggtgtctacg gcctcggtca gctcgtcggt gaaaagatcc tcgagcgcga 23460 ccactggtac tttccctgcc actttgtcaa ggatcaggtc atggccggat ccctcgtctc 23520 cgacggctgc agccagatgc tcaagatgta catgatctgg ctcggcctcc acctcaccac 23580 cggacccttt gacttccgcc cggtcaacgg ccaccccaac aaggtccgct gccgcggcca 23640 aatctccccg cacaagggca agctcgtcta cgtcatggag atcaaggaga tgggcttcga 23700 cgaggacaac gacccgtacg ccattgccga cgtcaacatc attgatgtcg acttcgaaaa 23760 gggccaggac tttagcctcg accgcatcag cgactacggc aagggcgacc tcaacaagaa 23820 gatcgtcgtc gactttaagg gcatcgctct caagatgcag aagcgctcca ccaacaagaa 23880 cccctccaag gttcagcccg tctttgccaa cggcgccgcc actgtcggcc ccgaggcctc 23940 caaggcttcc tccggcgcca gcgccagcgc cagcgccgcc ccggccaagc ctgccttcag 24000 cgccgatgtt cttgcgccca agcccgttgc ccttcccgag cacatcctca agggcgacgc 24060 cctcgccccc aaggagatgt cctggcaccc catggcccgc atcccgggca acccgacgcc 24120 ctcttttgcg ccctcggcct acaagccgcg caacatcgcc tttacgccct tccccggcaa 24180 ccccaacgat aacgaccaca ccccgggcaa gatgccgctc acctggttca acatggccga 24240 gttcatggcc ggcaaggtca gcatgtgcct cggccccgag ttcgccaagt tcgacgactc 24300 gaacaccagc cgcagccccg cttgggacct cgctctcgtc acccgcgccg tgtctgtgtc 24360 tgacctcaag cacgtcaact accgcaacat cgacctcgac ccctccaagg gtaccatggt 24420 cggcgagttc gactgccccg cggacgcctg gttctacaag ggcgcctgca acgatgccca 24480 catgccgtac tcgatcctca tggagatcgc cctccagacc tcgggtgtgc tcacctcggt 24540 gctcaaggcg cccctgacca tggagaagga cgacatcctc ttccgcaacc tcgacgccaa 24600 cgccgagttc gtgcgcgccg acctcgacta ccgcggcaag actatccgca acgtcaccaa 24660 gtgcactggc tacagcatgc tcggcgagat gggcgtccac cgcttcacct ttgagctcta 24720 cgtcgatgat gtgctctttt acaagggctc gacctcgttc ggctggttcg tgcccgaggt 24780 ctttgccgcc caggccggcc tcgacaacgg ccgcaagtcg gagccctggt tcattgagaa 24840 caaggttccg gcctcgcagg tctcctcctt tgacgtgcgc cccaacggca gcggccgcac 24900 cgccatcttc gccaacgccc ccagcggcgc ccagctcaac cgccgcacgg accagggcca 24960 gtacctcgac gccgtcgaca ttgtctccgg cagcggcaag aagagcctcg gctacgccca 25020 cggttccaag acggtcaacc cgaacgactg gttcttctcg tgccactttt ggtttgactc 25080 ggtcatgccc ggaagtctcg gtgtcgagtc catgttccag ctcgtcgagg ccatcgccgc 25140 ccacgaggat ctcgctggca agcacggcat tgccaacccc acctttgtgc acgccccggg 25200 caagatcagc tggaagtacc gcggccagct cacgcccaag agcaagaaga tggactcgga 25260 ggtccacatc gtgtccgtgg acgcccacga cggcgttgtc gacctcgtcg ccgacggctt 25320 cctctgggcc gacagcctcc gcgtctactc ggtgagcaac attcgcgtgc gcatcgcctc 25380 cggtgaggcc cctgccgccg cctcctccgc cgcctctgtg ggctcctcgg cttcgtccgt 25440 cgagcgcacg cgctcgagcc ccgctgtcgc ctccggcccg gcccagacca tcgacctcaa 25500 gcagctcaag accgagctcc tcgagctcga tgccccgctc tacctctcgc aggacccgac 25560 cagcggccag ctcaagaagc acaccgacgt ggcctccggc caggccacca tcgtgcagcc 25620 ctgcacgctc ggcgacctcg gtgaccgctc cttcatggag acctacggcg tcgtcgcccc 25680 gctgtacacg ggcgccatgg ccaagggcat tgcctcggcg gacctcgtca tcgccgccgg 25740 caagcgcaag atcctcggct cctttggcgc cggcggcctc cccatgcacc acgtgcgcgc 25800 cgccctcgag aagatccagg ccgccctgcc tcagggcccc tacgccgtca acctcatcca 25860 ctcgcctttt gacagcaacc tcgagaaggg caacgtcgat ctcttcctcg agaagggcgt 25920 cactgtggtg gaggcctcgg cattcatgac cctcaccccg caggtcgtgc gctaccgcgc 25980 cgccggcctc tcgcgcaacg ccgacggttc ggtcaacatc cgcaaccgca tcatcggcaa 26040 ggtctcgcgc accgagctcg ccgagatgtt catccgcccg gccccggagc acctcctcga 26100 gaagctcatc gcctcgggcg agatcaccca ggagcaggcc gagctcgcgc gccgcgttcc 26160 cgtcgccgac gatatcgctg tcgaggctga ctcgggcggc cacaccgaca accgccccat 26220 ccacgtcatc ctcccgctca tcatcaacct ccgcaaccgc ctgcaccgcg agtgcggcta 26280 ccccgcgcac ctccgcgtcc gcgttggcgc cggcggtggc gtcggctgcc cgcaggccgc 26340 cgccgccgcg ctcaccatgg gcgccgcctt catcgtcacc ggcactgtca accaggtcgc 26400 caagcagtcc ggcacctgcg acaacgtgcg caagcagctc tcgcaggcca cctactcgga 26460 tatctgcatg gccccggccg ccgacatgtt cgaggagggc gtcaagctcc aggtcctcaa 26520 gaagggaacc atgttcccct cgcgcgccaa caagctctac gagctctttt gcaagtacga 26580 ctccttcgac tccatgcctc ctgccgagct cgagcgcatc gagaagcgta tcttcaagcg 26640 cgcactccag gaggtctggg aggagaccaa ggacttttac attaacggtc tcaagaaccc 26700 ggagaagatc cagcgcgccg agcacgaccc caagctcaag atgtcgctct gcttccgctg 26760 gtaccttggt cttgccagcc gctgggccaa catgggcgcc ccggaccgcg tcatggacta 26820 ccaggtctgg tgtggcccgg ccattggcgc cttcaacgac ttcatcaagg gcacctacct 26880 cgaccccgct gtctccaacg agtacccctg tgtcgtccag atcaacctgc aaatcctccg 26940 tggtgcctgc tacctgcgcc gtctcaacgc cctgcgcaac gacccgcgca ttgacctcga 27000 gaccgaggat gctgcctttg tctacgagcc caccaacgcg ctctaatgag tagttagctt 27060 aatcacttag gtcaccggcc tagtaggccg gtcaccagta tgaactaaaa tgcatgtagg 27120 tgtaagagct catggagagc atggaatatt gtatccgacc atgtaacagt ataataactg 27180 agctccatct cacttcttct atgaataaac aaaggatgtt atgatatatt aacactctat 27240 ctatgcacct tattgttcta tgataaattt cctcttatta ttataaatca tctgaatcgt 27300 gacggcttat ggaatgcttc aaatagtaca aaaacaaatg tgtactataa gactttctaa 27360 acaattctaa ctttagcatt gtgaacgaga cataagtgtt aagaagacat aacaattata 27420 atggaagaag tttgtctcca tttatatatt atatattacc cacttatgta ttatattagg 27480 atgttaagga gacataacaa ttataaagag agaagtttgt atccatttat atattatata 27540 ctacccattt atatattata cttatccact tatttaatgt ctttataagg tttgatccat 27600 gatatttcta atattttagt tgatatgtat atgaaaaggt actatttgaa ctctcttact 27660 ctgtataaag gttggatcat ccttaaagtg ggtctattta attttattgc ttcttacaga 27720 taaaaaaaaa attatgagtt ggtttgataa aatattgaag gatttaaaat aataataaat 27780 aataaataac atataatata tgtatataaa tttattataa tataacattt atctataaaa 27840 aagtaaatat tgtcataaat ctatacaatc gtttagcctt gctggaacga atctcaatta 27900 tttaaacgag agtaaacata tttgactttt tggttattta acaaattatt atttaacact 27960 atatgaaatt tttttttttt atcagcaaag aataaaatta aattaagaag gacaatggtg 28020 tcccaatcct tatacaacca acttccacaa gaaagtcaag tcagagacaa caaaaaaaca 28080 agcaaaggaa attttttaat ttgagttgtc ttgtttgctg cataatttat gcagtaaaac 28140 actacacata acccttttag cagtagagca atggttgacc gtgtgcttag cttcttttat 28200 tttatttttt tatcagcaaa gaataaataa aataaaatga gacacttcag ggatgtttca 28260 acccttatac aaaaccccaa aaacaagttt cctagcaccc taccaacgaa ttcgcggccg 28320 cttaattaat cacgagtgga tgactgtcac gactgactca tgactgacta ctagtgctag 28380 cctcgaggtc gacacaactt tgtataataa agttgtctag acctaggcga gtcatgagtc 28440 gtgacatgac gtcatgctca tcagactgcg gccgcctccc agtatcatta tagtgaaagt 28500 tttggctctc tcgccggtgg ttttttacct ctatttaaag gggttttcca cctaaaaatt 28560 ctggtatcat tctcacttta cttgttactt taatttctca taatctttgg ttgaaattat 28620 cacgcttccg cacacgatat ccctacaaat ttattatttg ttaaacattt tcaaaccgca 28680 taaaatttta tgaagtcccg tctatcttta atgtagtcta acattttcat attgaaatat 28740 ataatttact taattttagc gttggtagaa agcataatga tttattctta ttcttcttca 28800 tataaatgtt taatatacaa tataaacaaa ttctttacct taagaaggat ttcccatttt 28860 atattttaaa aatatattta tcaaatattt ttcaaccacg taaatctcat aataataagt 28920 tgtttcaaaa gtaataaaat ttaactccat aattttttta ttcgactgat cttaaagcaa 28980 cacccagtga cacaactagc catttttttc tttgaataaa aaaatccaat tatcattgta 29040 ttttttttat acaatgaaaa tttcaccaaa caatgatttg tggtatttct gaagcaagtc 29100 atgttatgca aaattctata attcccattt gacactacgg aagtaactga agatctgctt 29160 ttacatgcga gacacatctt ctaaagtaat tttaataata gttactatat tcaagatttc 29220 atatatcaaa tactcaatat tacttctaaa aaattaatta gatataatta aaatattact 29280 tttttaattt taagtttaat tgttgaattt gtgactattg atttattatt ctactatgtt 29340 taaattgttt tatagatagt ttaaagtaaa tataagtaat gtagtagagt gttagagtgt 29400 taccctaaac cataaactat aagatttatg gtggactaat tttcatatat ttcttattgc 29460 ttttaccttt tcttggtatg taagtccgta actggaatta ctgtgggttg ccatgacact 29520 ctgtggtctt ttggttcatg catggatctt gcgcaagaaa aagacaaaga acaaagaaaa 29580 aagacaaaac agagagacaa aacgcaatca cacaaccaac tcaaattagt cactggctga 29640 tcaagatcgc cgcgtccatg tatgtctaaa tgccatgcaa agcaacacgt gcttaacatg 29700 cactttaaat ggctcaccca tctcaaccca cacacaaaca cattgccttt ttcttcatca 29760 tcaccacaac cacctgtata tattcattct cttccgccac ctcaatttct tcacttcaac 29820 acacgtcaac ctgcatatgc gtgtcatccc atgcccaaat ctccatgcat gttccaacca 29880 ccttctctct tatataatac ctataaatac ctctaatatc actcacttct ttcatcatcc 29940 atccatccag agtactacta ctctactact ataatacccc aacccaactc atattcaata 30000 ctactctagg atccggccat ctaggccgga tccacaacca aaaatggctc ccactcccga 30060 cgccaccgcg cctctgaaca agccgagcga ctatgccgtc taccacgagg aagacggccc 30120 cttctggacc gccgattcca gcggcgtctc gcgcgtgaac tttagcgaga ccggcgtggg 30180 atccgagggc gtcatccctg cgctcacgct catcgacgtc ttcgagaggg ccgtcaagcg 30240 cggcggaaac aggatcgcct tccgcacgga aaacatgccc acgctccgcc gcggcgaaga 30300 ggccccggac gcgctgccgc tcaaggactg gaagtcctgg tcctggaaac agtacaaggc 30360 cgacgtccac cgcatcgcca gggctctcat ggacctcggc gttgagcagc atgacgccgt 30420 ctccattttt ggctttaact cgcccgaatg gtttctcagt gccgtcggcg ccgtgcacgc 30480 aggtgccaag attgccggca tttacccctc agacacgccc gcccaggtcc agtacaaggc 30540 cttccacagt gacaccgctg ttgccgttgt cgaaaacgag cagtgcttca agaagttcgc 30600 cgaggtcgtc gaggaccttc cttacctcaa ggccattgtt tgctgggact atgaagccac 30660 agacatcacg cgcgaggacg gctccgtcgt cgaggtcctc acctttgccg agttcctcaa 30720 gcgcggcgac accgtcgagg cggccgccct tgacgagcgc atctccaaga tcgagcccac 30780 catgtgcgct gcccttattt acaccagcgg tactaccggc cgccccaagg ccgttatgat 30840 ttcgcacgac aaccttgttt tcgaggccag cgccgtcgtc cccaacctcg gaggagcctg 30900 tacgaccact gctgaggagc gcattctctc gtacctgcct ctctcgcacg tcgctggtat 30960 gatggttgat attattgccc ccatcattgc caccgccttc cacaagggcc gcatctgcgt 31020 ctgctttgct cgcccgtacg atttgcgcac cggcacgctg ggccagcgcc tcaacgccgt 31080 ggagcccacc atcttccttg gcgtgccccg tgtgtgggaa aagattcagg aaaagctcat 31140 ggccgtcggt gccaaaacca ccggcctcaa gaagaagctc tctacggccg ccaagaagcg 31200 tggtcttgaa ttccaggagg agcagcaaat cggccgctcc ggtgccaacc ctggctttgg 31260 cccccttggc atctacaaga agctcctcgg cctcatcaag ggcaagctgg gcctcaccaa 31320 gtgcaagttt gcctttgctg gtgccgcgcc catgacccgt gagacccttc agtactttgg 31380 cgcgctgaac atcaacatta acgaggtcta cggcatgtcc gagtgctccg gtgccgccac 31440 ctggtccacg gacaaggccc acgagtgggg cactgttggc tacgagatgc ccagttgcga 31500 ggtccgcgtc ttcaagattg ccgaggacgg taccaagacc gagtgcccgc gcgccgccga 31560 cattatgcat gctaccgagg aggagcaggg cgaagtttgc ttccgcggcc gtaacatcat 31620 gatgggctac cttgccaacc ccaagcttgg cgacgaccac gttgccgaga tcgaggagaa 31680 gaacgctgcc gctatcgact ccgagggctg gctccacagt ggtgataagg gcgccatttc 31740 tacccgcggc atgctcaaga tcacgggccg ctacaaggag ctcatcatcg gcgccggtgg 31800 cgagaacgtg gcgcccgtcc ctattgagga cgccatcaag gcgcgcatgc cttttgtttc 31860 caacgccatg atggtcggag ataagcgcaa gttcatggct gtcctcctta ccctcaagac 31920 ggttggcgcc acgggcgagc ttcccggtac gaacaagctc atgggcgctg ccgccgacta 31980 tggtgagacc atcgaggacg cctgcgacaa cgaggcgctc attgaggaga tcacgcagca 32040 gctcaaggag atcggtgatg atggcgatgt cacgccctcg aacgcggctc gcatccaaaa 32100 gttcaccatt ctcccgctcg acttttccgt ctccacggac gagctcacgg ccacgctcaa 32160 gctcaagcgc tccgtggtcg cagacaagta cgaagacatc atcgaggcct tttacgagtc 32220 caagagcgtt tttgtgccgt actcgaccgt tggcgcctac gccacgggcg gcccggtcga 32280 cgactccgtt gtcgatggct ccttcaaggg cgactttagc atgattggcg acgatgatcc 32340 ggatcttcaa aacgtcgatg tcctcgaggc gattgacgag gacaattaaa tttgagtagt 32400 tagcttaatc acttaggtca ccggcctagt aggccggtca ccagtatgaa ctaaaatgca 32460 tgtaggtgta agagctcatg gagagcatgg aatattgtat ccgaccatgt aacagtataa 32520 taactgagct ccatctcact tcttctatga ataaacaaag gatgttatga tatattaaca 32580 ctctatctat gcaccttatt gttctatgat aaatttcctc ttattattat aaatcatctg 32640 aatcgtgacg gcttatggaa tgcttcaaat agtacaaaaa caaatgtgta ctataagact 32700 ttctaaacaa ttctaacttt agcattgtga acgagacata agtgttaaga agacataaca 32760 attataatgg aagaagtttg tctccattta tatattatat attacccact tatgtattat 32820 attaggatgt taaggagaca taacaattat aaagagagaa gtttgtatcc atttatatat 32880 tatatactac ccatttatat attatactta tccacttatt taatgtcttt ataaggtttg 32940 atccatgata tttctaatat tttagttgat atgtatatga aaaggtacta tttgaactct 33000 cttactctgt ataaaggttg gatcatcctt aaagtgggtc tatttaattt tattgcttct 33060 tacagataaa aaaaaaatta tgagttggtt tgataaaata ttgaaggatt taaaataata 33120 ataaataata aataacatat aatatatgta tataaattta ttataatata acatttatct 33180 ataaaaaagt aaatattgtc ataaatctat acaatcgttt agccttgctg gaacgaatct 33240 caattattta aacgagagta aacatatttg actttttggt tatttaacaa attattattt 33300 aacactatat gaaatttttt ttttttatca gcaaagaata aaattaaatt aagaaggaca 33360 atggtgtccc aatccttata caaccaactt ccacaagaaa gtcaagtcag agacaacaaa 33420 aaaacaagca aaggaaattt tttaatttga gttgtcttgt ttgctgcata atttatgcag 33480 taaaacacta cacataaccc ttttagcagt agagcaatgg ttgaccgtgt gcttagcttc 33540 ttttatttta tttttttatc agcaaagaat aaataaaata aaatgagaca cttcagggat 33600 gtttcaaccc ttatacaaaa ccccaaaaac aagtttccta gcaccctacc aacgaattcg 33660 cggccgctta attaatctga tcatggatgc tacgtcacgg cagtacagga ctatcactag 33720 acctaggagc tgtgctgggt aactcacatg agtcactggt cacatcagcg gccgcctccc 33780 agtatcatta tagtgaaagt tttggctctc tcgccggtgg ttttttacct ctatttaaag 33840 gggttttcca cctaaaaatt ctggtatcat tctcacttta cttgttactt taatttctca 33900 taatctttgg ttgaaattat cacgcttccg cacacgatat ccctacaaat ttattatttg 33960 ttaaacattt tcaaaccgca taaaatttta tgaagtcccg tctatcttta atgtagtcta 34020 acattttcat attgaaatat ataatttact taattttagc gttggtagaa agcataatga 34080 tttattctta ttcttcttca tataaatgtt taatatacaa tataaacaaa ttctttacct 34140 taagaaggat ttcccatttt atattttaaa aatatattta tcaaatattt ttcaaccacg 34200 taaatctcat aataataagt tgtttcaaaa gtaataaaat ttaactccat aattttttta 34260 ttcgactgat cttaaagcaa cacccagtga cacaactagc catttttttc tttgaataaa 34320 aaaatccaat tatcattgta ttttttttat acaatgaaaa tttcaccaaa caatgatttg 34380 tggtatttct gaagcaagtc atgttatgca aaattctata attcccattt gacactacgg 34440 aagtaactga agatctgctt ttacatgcga gacacatctt ctaaagtaat tttaataata 34500 gttactatat tcaagatttc atatatcaaa tactcaatat tacttctaaa aaattaatta 34560 gatataatta aaatattact tttttaattt taagtttaat tgttgaattt gtgactattg 34620 atttattatt ctactatgtt taaattgttt tatagatagt ttaaagtaaa tataagtaat 34680 gtagtagagt gttagagtgt taccctaaac cataaactat aagatttatg gtggactaat 34740 tttcatatat ttcttattgc ttttaccttt tcttggtatg taagtccgta actggaatta 34800 ctgtgggttg ccatgacact ctgtggtctt ttggttcatg catggatctt gcgcaagaaa 34860 aagacaaaga acaaagaaaa aagacaaaac agagagacaa aacgcaatca cacaaccaac 34920 tcaaattagt cactggctga tcaagatcgc cgcgtccatg tatgtctaaa tgccatgcaa 34980 agcaacacgt gcttaacatg cactttaaat ggctcaccca tctcaaccca cacacaaaca 35040 cattgccttt ttcttcatca tcaccacaac cacctgtata tattcattct cttccgccac 35100 ctcaatttct tcacttcaac acacgtcaac ctgcatatgc gtgtcatccc atgcccaaat 35160 ctccatgcat gttccaacca ccttctctct tatataatac ctataaatac ctctaatatc 35220 actcacttct ttcatcatcc atccatccag agtactacta ctctactact ataatacccc 35280 aacccaactc atattcaata ctactctagg atccacaacc aaaaatgttg cagcatactt 35340 ggctaccaaa acccccaaat ttaaccttat tgtcagatga agttcatctc tggcgcattc 35400 cccttgacca accagaatca cagctacagg atttagccgc taccttatct agtgacgaat 35460 tagcccgtgc aaacagattt tattttcccg aacatcgccg gcgttttact gctggtcgtg 35520 gtattctccg cagtatcttg gggggctatt tgggtgtgga accagggcaa gttaaatttg 35580 attatgaatc ccgtggtaaa ccaatattag gcgatcgctt tgccgagagt ggtttattat 35640 ttaacttgtc acactcccag aacttggcct tgtgtgcagt caattacacg cgccaaatcg 35700 gcatcgattt agaatatctc cgccccacat ctgatttaga atcccttgcc aaaaggttct 35760 ttttaccgcg agaatatgaa ttattgcgat cgctacccga tgagcaaaaa caaaaaattt 35820 tctttcgtta ctggacttgt aaagaggctt atcttaaagc aacgggtgac ggcatcgcta 35880 aattagagga aattgaaata gcactaactc ccacagaacc agctaagtta cagacagctc 35940 cagcgtggag tctcctagag ctagtgccag atgataattg tgttgctgct gttgccgtgg 36000 cgggttttgg ctggcagcca aaattctggc attattgagt agttagctta atcacttagg 36060 tcaccagtat gaactaaaat gcatgtaggt gtaagagctc atggagagca tggaatattg 36120 tatccgacca tgtaacagta taataactga gctccatctc acttcttcta tgaataaaca 36180 aaggatgtta tgatatatta acactctatc tatgcacctt attgttctat gataaatttc 36240 ctcttattat tataaatcat ctgaatcgtg acggcttatg gaatgcttca aatagtacaa 36300 aaacaaatgt gtactataag actttctaaa caattctaac tttagcattg tgaacgagac 36360 ataagtgtta agaagacata acaattataa tggaagaagt ttgtctccat ttatatatta 36420 tatattaccc acttatgtat tatattagga tgttaaggag acataacaat tataaagaga 36480 gaagtttgta tccatttata tattatatac tacccattta tatattatac ttatccactt 36540 atttaatgtc tttataaggt ttgatccatg atatttctaa tattttagtt gatatgtata 36600 tgaaaaggta ctatttgaac tctcttactc tgtataaagg ttggatcatc cttaaagtgg 36660 gtctatttaa ttttattgct tcttacagat aaaaaaaaaa ttatgagttg gtttgataaa 36720 atattgaagg atttaaaata ataataaata ataaataaca tataatatat gtatataaat 36780 ttattataat ataacattta tctataaaaa agtaaatatt gtcataaatc tatacaatcg 36840 tttagccttg ctggaacgaa tctcaattat ttaaacgaga gtaaacatat ttgacttttt 36900 ggttatttaa caaattatta tttaacacta tatgaaattt ttttttttta tcagcaaaga 36960 ataaaattaa attaagaagg acaatggtgt cccaatcctt atacaaccaa cttccacaag 37020 aaagtcaagt cagagacaac aaaaaaacaa gcaaaggaaa ttttttaatt tgagttgtct 37080 tgtttgctgc ataatttatg cagtaaaaca ctacacataa cccttttagc agtagagcaa 37140 tggttgaccg tgtgcttagc ttcttttatt ttattttttt atcagcaaag aataaataaa 37200 ataaaatgag acacttcagg gatgtttcaa cccttataca aaaccccaaa aacaagtttc 37260 ctagcaccct accaacgaat tcgcggccgc ttaattaact actgtcactg aggccgtaga 37320 cgagtacgga ctgatctaac tagtgctagc ctcgaggtcg acacccagct ttcttgtaca 37380 aagtggttgc ggccgcttaa ttaaatttaa attcaattaa tgcaatcttg attttcaaca 37440 acgaaggtaa tggcgtaaaa gaaaaaatgt atgttattgt attgatcttt catgatgttg 37500 aagcgtgcca taatatgatg atgtataatt aaaatattaa ctgtcgcatt ttattgaaat 37560 ggcactgtta tttcaaccat atctttgatt ctgttacatg acacgactgc aagaagtaaa 37620 taatagacgc cgttgttaaa gaattgctat catatgtgcc taactagagg gaatttgagc 37680 gtcagaccta atcaaatatt acaaaatatc tcactctgtc gccagcaatg gtgtaatcag 37740 cgcagacaaa tggcgtaaag atcgcggaaa aacctccccg agtggcatga tagctgcctc 37800 tgtattgctg atttagtcag ccttatttga cttaagggtg ccctcgttag tgacaaattg 37860 ctttcaagga gacagccatg ccccacactt tgttgaaaaa caaattgcct ttggggagac 37920 ggtaaagcca gttgctcttc aataaggaat gtcgaggagg caatgtaacc gcctctggta 37980 gtacacttct ctaatccaaa aatcaatttg tattcaagat accgcaaaaa acttatggtt 38040 taaaccctgc aggactagtc cagaaggtaa ttatccaaga tgtagcatca agaatccaat 38100 gtttacggga aaaactatgg aagtattatg taagctcagc aagaagcaga tcaatatgcg 38160 gcacatatgc aacctatgtt caaaaatgaa gaatgtacag atacaagatc ctatactgcc 38220 agaatacgaa gaagaatacg tagaaattga aaaagaagaa ccaggcgaag aaaagaatct 38280 tgaagacgta agcactgacg acaacaatga aaagaagaag ataaggtcgg tgattgtgaa 38340 agagacatag aggacacatg taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca 38400 aaggaatctt atcccccact acttatcctt ttatattttt ccgtgtcatt tttgcccttg 38460 agttttccta tataaggaac caagttcggc atttgtgaaa acaagaaaaa atttggtgta 38520 agctattttc tttgaagtac tgaggataca acttcagaga aatttgtaag tttgtaggta 38580 ccagatctgg atcccaaacc atgtctccgg agaggagacc agttgagatt aggccagcta 38640 cagcagctga tatggccgcg gtttgtgata tcgttaacca ttacattgag acgtctacag 38700 tgaactttag gacagagcca caaacaccac aagagtggat tgatgatcta gagaggttgc 38760 aagatagata cccttggttg gttgctgagg ttgagggtgt tgtggctggt attgcttacg 38820 ctgggccctg gaaggctagg aacgcttacg attggacagt tgagagtact gtttacgtgt 38880 cacataggca tcaaaggttg ggcctaggat ctacattgta cacacatttg cttaagtcta 38940 tggaggcgca aggttttaag tctgtggttg ctgttatagg ccttccaaac gatccatctg 39000 ttaggttgca tgaggctttg ggatacacag cccggggtac attgcgcgca gctggataca 39060 agcatggtgg atggcatgat gttggttttt ggcaaaggga ttttgagttg ccagctcctc 39120 caaggccagt taggccagtt acccaaatct gagtagttag cttaatcacc tagagctcga 39180 tcggcggcaa tagcttctta gcgccatccc gggttgatcc tatctgtgtt gaaatagttg 39240 cggtgggcaa ggctctcttt cagaaagaca ggcggccaaa ggaacccaag gtgaggtggg 39300 ctatggctct cagttccttg tggaagcgct tggtctaagg tgcagaggtg ttagcgggat 39360 gaagcaaaag tgtccgattg taacaagata tgttgatcct acgtaaggat attaaagtat 39420 gtattcatca ctaatataat cagtgtattc caatatgtac tacgatttcc aatgtcttta 39480 ttgtcgccgt atgtaatcgg cgtcacaaaa taatccccgg tgactttctt ttaatccagg 39540 atgaaataat atgttattat aatttttgcg atttggtccg ttataggaat tgaagtgtgc 39600 ttgaggtcgg tcgccaccac tcccatttca taattttaca tgtatttgaa aaataaaaat 39660 ttatggtatt caatttaaac acgtatactt gtaaagaatg atatcttgaa agaaatatag 39720 tttaaatatt tattgataaa ataacaagtc aggtattata gtccaagcaa aaacataaat 39780 ttattgatgc aagtttaaat tcagaaatat ttcaataact gattatatca gctggtacat 39840 tgccgtagat gaaagactga gtgcgatatt atggtgtaat acataggaat tcgtttaaac 39900 gatctgcgtc taattttcgg tccaacttgc acaggaaaga cgtcgaccgc ggtagctctt 39960 gcccagcaga ctgggcttcc agtcctttcg ctcgatcggg tccaatgttg tcctcagctg 40020 tgaaccggaa gcggacgacc aacagtggaa gaactgaaag gaacgagccg tctatacctt 40080 gatgatcggc ctctggtgaa gggtatcatc gcagccaagc aagctcatga aaggctgatg 40140 ggggaggtgt ataattatga ggcccacggc gggcttattc tttagggagg atctatctcg 40200 ttgctcaagt gcatggcgca aagcagttat tggagtgcgg attttcgttg gcatattatt 40260 cgccacgagt tagcagacga agagaccttc atgaacgtgg ccaaggccag agttaagcag 40320 atgttacgcc ctgctgcagg cctttctatt atccaatagt tggttgatct ttggaaagag 40380 cctcggctga ggcccatact gaaagagatc gatggatatc gatatgccat gttgtttgct 40440 agccagaacc agatcacatc cgatatgcta ttgcagcttg acgcagatat ggaggataag 40500 ttgattcatg ggatcgctca ggagtagctc atccatgcac gccgacaaga acagaaattc 40560 cgtcgagtta acgcagccgc ttacgacgga ttcgaaggtc atccattcgg aatgtattag 40620 tttgcaccag ctccgcgtca cacctgtctt catttgaata agatgttagc aattgttttt 40680 agctttgtct tgttgtggca gggcggcaag tgcttcagac atcattctgt tttcaaattt 40740 tatgctggag aacagcttct taattccttt ggaaataata gactgcgtct taaaattcag 40800 atgtctggat atagatatga ttgtaaaata acctatttaa gtgtcattta gaacataagt 40860 tttatgaatg ttcttccatt ttcgtcatcg aacgaataag agtaaataca ccttttttaa 40920 cattacaaat aagttcttat acgttgttta tacaccggga atcatttcca ttattttcgc 40980 gcaaaagtca cggatattcg tgaaagcgac ataaactgcg aaatttgcgg ggagtgtctt 41040 gagtttgcct cgaggctagc gcatgcacat agacacacac atcatctcat tgatgcttgg 41100 taataattgt cattagattg tttttatgca tagatgcact cgaaatcagc caattttaga 41160 caagtatcaa acggatgtga cttcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt 41220 gtctaagcgt caatttgatt tacaattgaa tatatcctgc cccagccagc caacagctcg 41280 atttacaatt gaatatatcc tgccggccgg cccacgcgtg tcgaggaatt ctgatctggc 41340 ccccatttgg acgtgaatgt agacacgtcg aaataaagat ttccgaatta gaataatttg 41400 tttattgctt tcgcctataa atacgacgga tcgtaatttg tcgttttatc aaaatgtact 41460 ttcattttat aataacgctg cggacatcta catttttgaa ttgaaaaaaa attggtaatt 41520 actctttctt tttctccata ttgaccatca tactcattgc tgatccatgt agatttcccg 41580 gacatgaagc catttacaat tgaatatatc ctgccgccgc tgccgctttg cacccggtgg 41640 agcttgcatg ttggtttcta cgcagaactg agccggttag gcagataatt tccattgaga 41700 actgagccat gtgcaccttc cccccaacac ggtgagcgac ggggcaacgg agtgatccac 41760 atgggacttt taaacatcat ccgtcggatg gcgttgcgag agaagcagtc gatccgtgag 41820 atcagccgac gcaccgggca ggcgcgcaac acgatcgcaa agtatttgaa cgcaggtaca 41880 atcgagccga cgttcacgcg gaacgaccaa gcaagcttgg ctgccatttt tggggtgagg 41940 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg 42000 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca 42060 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta 42120 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag 42180 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa 42240 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact 42300 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc 42360 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt 42420 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt 42480 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg 42540 cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag 42600 ggcaaccagc ccggtgagcg tcggaaaggg tcgacggatc ttttccgctg cataaccctg 42660 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg 42720 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag 42780 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca 42840 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac 42900 agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat 42960 caaggtgtac tgccttccag acgaacgaag agcgattgag gaaaaggcgg cggcggccgg 43020 catgagcctg tcggcctacc tgctggccgt cggccagggc tacaaaatca cgggcgtcgt 43080 ggactatgag cacgtccgcg agctggcccg catcaatggc gacctgggcc gcctgggcgg 43140 cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg cggttcggtg atgccacgat 43200 cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag cttggcaagg tcatgatggg 43260 cgtggtccgc ccgagggcag agccatgact tttttagccg ctaaaacggc cggggggtgc 43320 gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa gagcgacttc gcggagctgg 43380 tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg 43440 ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa 43500 atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa 43560 tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg 43620 ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc 43680 agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac 43740 tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac 43800 aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca 43860 agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg 43920 cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg 43980 cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca 44040 ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca 44100 acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg 44160 tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca 44220 cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg 44280 ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg 44340 ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa 44400 cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact 44460 acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg 44520 actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt 44580 gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag 44640 agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca 44700 aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagccagc gctttactgg 44760 catttcagga acaagcgggc actgctcgac gcacttgctt cgctcagtat cgctcgggac 44820 gcacggcgcg ctctacgaac tgccgataaa cagaggatta aaattgacaa ttgtgattaa 44880 ggctcagatt cgacggcttg gagcggccga cgtgcaggat ttccgcgaga tccgattgtc 44940 ggccctgaag aaagctccag agatgttcgg gtccgtttac gagcacgagg agaaaaagcc 45000 catggaggcg ttcgctgaac ggttgcgaga tgccgtggca ttcggcgcct acatcgacgg 45060 cgagatcatt gggctgtcgg tcttcaaaca ggaggacggc cccaaggacg ctcacaaggc 45120 gcatctgtcc ggcgttttcg tggagcccga acagcgaggc cgaggggtcg ccggtatgct 45180 gctgcgggcg ttgccggcgg gtttattgct cgtgatgatc gtccgacaga ttccaacggg 45240 aatctggtgg atgcgcatct tcatcctcgg cgcacttaat atttcgctat tctggagctt 45300 gttgtttatt tcggtctacc gcctgccggg cggggtcgcg gcgacggtag gcgctgtgca 45360 gccgctgatg gtcgtgttca tctctgccgc tctgctaggt agcccgatac gattgatggc 45420 ggtcctgggg gctatttgcg gaactgcggg cgtggcgctg ttggtgttga caccaaacgc 45480 agcgctagat cctgtcggcg tcgcagcggg cctggcgggg gcggtttcca tggcgttcgg 45540 aaccgtgctg acccgcaagt ggcaacctcc cgtgcctctg ctcaccttta ccgcctggca 45600 actggcggcc ggaggacttc tgctcgttcc agtagcttta gtgtttgatc cgccaatccc 45660 gatgcctaca ggaaccaatg ttctcggcct ggcgtggctc ggcctgatcg gagcgggttt 45720 aacctacttc ctttggttcc gggggatctc gcgactcgaa cctacagttg tttccttact 45780 gggctttctc agcccccgag cgcttagtgg gaatttgtac cccttatcga accgggagca 45840 caggatgacg cctaacaatt cattcaagcc gacaccgctt cgcggcgcgg cttaattcag 45900 gagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta 45960 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc 46020 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta 46080 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc 46140 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc 46200 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac 46260 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca 46320 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg 46380 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg 46440 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc 46500 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg 46560 ccggcccagt atcagcccgt catacttgaa gctaggcagg cttatcttgg acaagaagat 46620 cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc 46680 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg 46740 cggcttaact caagcgttag agagctgggg aagactatgc gcgatctgtt gaaggtggtt 46800 ctaagcctcg tcttgcgatg gcatttcgat ccattcccat tccgcgctca agatggcttc 46860 ccctcggcag ttcatcaggg ctaaatcaat ctagccgact tgtccggtga aatgggctgc 46920 actccaacag aaacaatcaa acaaacatac acagcgactt attcacacga gctcaaatta 46980 caacggtata tatcctgcca gtcagcatca tcacaccaaa agttaggccc gaatagtttg 47040 aaattagaaa gctcgcaatt gaggtctaca ggccaaattc gctcttagcc gtacaatatt 47100 actcaccgga tcctaaccgg tttaattaag gcgcgccatg cccgggcaag cggccgcaca 47160 agttt 47165 <210> 51 <211> 46978 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9330 <400> 51 gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat ctcagcatgt 60 gcgtagcacg cggccgcctc ccagtatcat tatagtgaaa gttttggctc tctcgccggt 120 ggttttttac ctctatttaa aggggttttc cacctaaaaa ttctggtatc attctcactt 180 tacttgttac tttaatttct cataatcttt ggttgaaatt atcacgcttc cgcacacgat 240 atccctacaa atttattatt tgttaaacat tttcaaaccg cataaaattt tatgaagtcc 300 cgtctatctt taatgtagtc taacattttc atattgaaat atataattta cttaatttta 360 gcgttggtag aaagcataat gatttattct tattcttctt catataaatg tttaatatac 420 aatataaaca aattctttac cttaagaagg atttcccatt ttatatttta aaaatatatt 480 tatcaaatat ttttcaacca cgtaaatctc ataataataa gttgtttcaa aagtaataaa 540 atttaactcc ataatttttt tattcgactg atcttaaagc aacacccagt gacacaacta 600 gccatttttt tctttgaata aaaaaatcca attatcattg tatttttttt atacaatgaa 660 aatttcacca aacaatgatt tgtggtattt ctgaagcaag tcatgttatg caaaattcta 720 taattcccat ttgacactac ggaagtaact gaagatctgc ttttacatgc gagacacatc 780 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 840 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 900 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 960 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 1020 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 1080 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 1140 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 1200 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 1260 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 1320 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 1380 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 1440 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 1500 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 1560 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 1620 ccaaaaatgg cagcgaggct gcaagagcag aaaggaggtg agatggacac gaggattgcg 1680 atcataggca tgagcgccat acttccttgt ggaaccacgg tgagagagtc atgggagacc 1740 ataagggctg gcattgattg cctgtctgat cttcctgagg atcgtgttga tgtgactgcg 1800 tactttgacc cagtcaagac cacaaaggac aaaatctact gcaagagagg aggtttcatt 1860 cctgagtatg actttgatgc gcgtgagttt ggtctcaaca tgttccagat ggaagattct 1920 gatgcgaatc agaccatctc acttctcaag gtcaaagaag cacttcaaga tgctgggatt 1980 gatgcacttg gaaaggagaa gaaaaacata ggatgcgtgc ttggcattgg aggtggacag 2040 aagagttcac atgagttcta cagcagactc aactatgtgg ttgtggagaa agtcttgaga 2100 aagatgggga tgccagagga agatgtcaaa gtggctgtgg agaagtacaa agcgaacttc 2160 ccagaatgga gacttgactc tttccctgga ttccttggga atgtcactgc tggcagatgc 2220 accaacacat tcaacttgga tggcatgaac tgcgttgtgg atgctgcatg tgcaagctcc 2280 ttgatagcag tcaaggttgc gattgatgaa ctgctctatg gtgactgcga catgatggtc 2340 actggtgcaa cctgcacaga caactccatt gggatgtaca tggctttctc aaagacacct 2400 gtgttcagca cggacccaag tgtgagagcg tatgatgaga aaacgaaagg gatgctcatt 2460 ggtgagggat cagccatgtt ggtgctcaaa cgttatgcag atgcagtgag ggatggtgat 2520 gagattcatg ctgtgattcg tggttgtgca agctcaagtg atggcaaagc agctggcatc 2580 tacactccca caatcagtgg tcaagaggaa gccttgagac gtgcatacaa tcgtgcctgc 2640 gttgaccctg ccactgtcac tttggttgag ggtcatggga ctggcactcc agttggagat 2700 aggattgagt tgactgcgtt gaggaacttg tttgacaaag cgtatggtga agggaacaca 2760 gagaaagttg cagttggaag tatcaagtcc agtattggtc atttgaaggc tgttgctggt 2820 cttgctggaa tgatcaaggt catcatggct ctcaaacaca agactttgcc tggaacgatc 2880 aatgtggaca atccacccaa tctctatgac aacactccca tcaatgagtc atccctctac 2940 atcaacacga tgaacagacc ctggttccct ccacctggtg ttccgagacg tgctggaata 3000 tccagctttg gctttggagg tgccaactac catgcagtgt tggaagaggc tgaaccagag 3060 cacacgacag cgtacagact caacaaacgt ccacaaccgg tgttgatgat ggctgcaact 3120 cctgctgcgt tgcagagcct ttgtgaagct cagctgaaag agtttgaagc tgcgatcaag 3180 gagaatgaga cagtcaagaa tactgcctac atcaagtgtg tcaagtttgg agaacagttc 3240 aagttccctg ggagcatacc tgcgaccaat gcgagactgg gattcttggt caaagatgcg 3300 gaggatgcgt gctccacgct tcgtgctata tgtgctcagt ttgccaaaga tgtgaccaaa 3360 gaagcgtgga ggcttccgag agaaggagtt tctttccgtg ccaaaggcat tgccaccaat 3420 ggtgctgttg ctgcactctt ctctggacaa ggtgctcagt acacacacat gttcagtgaa 3480 gttgcgatga actggcctca gttcagacag tccattgctg caatggacgc tgcacagtcc 3540 aaggttgctg gaagtgacaa agactttgaa agagtctctc aagtcctgta cccaaggaaa 3600 ccgtacgaac gtgagccaga acaagatcac aagaaaatct cactcacagc atacagccag 3660 ccgtcaaccc tggcatgtgc tcttggtgcc tttgagattt tcaaggaagc tggattcact 3720 ccagactttg cagctggaca cagcttgggt gagtttgctg cgctctatgc agctggttgt 3780 gttgacagag atgagttgtt tgaacttgtt tgcagaaggg caaggatcat gggaggcaaa 3840 gatgcacctg ccacacccaa aggttgcatg gcagctgtca taggacccaa cgcagagaac 3900 atcaaggttc aagctgccaa cgtctggctt ggaaactcaa actctccgtc tcagactgtc 3960 attactgggt ctgttgaagg gattcaagct gaaagtgcca gattgcagaa ggaagggttc 4020 agagttgtgc ctcttgcttg tgagtctgcc ttccattctc cacagatgga aaatgccagc 4080 tcagctttca aagatgtcat atccaaggtt tccttccgta cacccaaagc agaaacaaag 4140 ctcttcagca atgtttctgg agagacctat ccgacggatg ccagagagat gctcactcaa 4200 cacatgacct catctgtgaa gttcctgacc caagtgagga acatgcacca agctggtgcg 4260 agaatctttg ttgagtttgg accgaaacaa gttctcagca aacttgtttc tgaaaccctc 4320 aaagatgacc cgtctgttgt cacagtgtct gtgaacccag cttctggcac ggactctgac 4380 atacagttga gggatgctgc agttcagctg gttgtggctg gtgtcaatct tcaagggttt 4440 gacaagtggg atgctcctga tgccacgagg atgcaagcca tcaagaaaaa gagaaccaca 4500 ctcagacttt cagctgccac ctatgtcagt gacaagacca agaaagtgag ggatgctgcc 4560 atgaacgacg gaagatgtgt cacatacctc aagggtgctg caccactcat caaggctcct 4620 gaacctgtgg ttgacgaagc tgccaagagg gaggctgaaa ggcttcagaa ggagcttcaa 4680 gacgctcaga ggcagcttga cgacgccaaa cgtgcagctg cagaagccaa ctccaaactt 4740 gctgctgcca aggaggaagc caagactgct gcagcttcag ccaagcctgc agttgacact 4800 gctgttgtgg agaaacatcg tgccatcctc aagtccatgc tggctgagct ggatggttat 4860 ggaagtgttg atgcttcttc ccttcagcaa cagcaacagc aacagactgc tccggcgcct 4920 gttaaagccg ctgcccctgc cgctcccgtt gcaagtgctc ctgctcccgc cgtgtccaac 4980 gagttgcttg agaaagctga gacagttgtg atggaggttc tcgctgccaa gactggatac 5040 gaaacagaca tgattgaggc ggacatggag cttgagactg agttgggaat tgacagcatc 5100 aagagggttg aaattctttc tgaagtccaa gctatgctta acgttgaggc gaaagatgtt 5160 gatgctctct ctaggacgag aacagtgggt gaagttgtca acgctatgaa ggcagaaatc 5220 gctggttctt ccgctccggc gccggcagct gccgcacctg caccggctaa agcggctcca 5280 gctgcagctg caccagctgt ttctaatgag ctgctcgaaa aggcagaaac tgttgtgatg 5340 gaagtccttg ctgccaaaac tggctacgaa actgacatga tagaatcaga catggagctt 5400 gagacggagc ttggaattga ttccatcaag cgtgtggaaa tcctcagtga agtccaagcc 5460 atgctcaatg tggaagctaa ggatgtggat gctctctcaa ggaccagaac tgtcggtgag 5520 gttgtcaatg ccatgaaggc tgagatagct ggaggctccg ctcctgcgcc ggccgctgcc 5580 gctcctggtc ccgctgccgc ggctccagct ccagctgcag ccgctccggc cgtctcaaac 5640 gaactccttg aaaaagctga gaccgtcgtg atggaggtct tggcagctaa aaccggctat 5700 gagactgaca tgattgagtc tgacatggaa ttggagacag aacttgggat agacagcatc 5760 aaacgtgttg aaatcctcag tgaggtccaa gctatgctga acgtggaagc gaaggatgtt 5820 gatgctctgt ccagaacgag gaccgttggt gaggttgtgg atgccatgaa ggctgaaatt 5880 gctggagggt ctgcgcccgc gccagctgca gccgctcctg cacccgcagc cgcggcacct 5940 gctccagctg cacctgcacc tgccgtttct tcagaactct tggagaaggc tgagacggtt 6000 gtgatggagg ttttggctgc aaagactggc tatgagaccg acatgattga atctgatatg 6060 gaattggaga cagagcttgg gatcgattcc atcaaaagag tggagatcct gagtgaagtc 6120 caagccatgc tcaatgttga agctaaggat gttgatgcac tttcaagaac aaggacagtg 6180 ggtgaggttg tggatgccat gaaggcggag attgctggag ggagcgcgcc tgcgccagct 6240 gccgcggcac ctgcaccggc agccgcagct ccagctccag ctgcaccagc accggccgcg 6300 cccgcgccag ctgtttcatc cgaactcctg gaaaaggccg agacggttgt gatggaggtg 6360 ctcgcagcta agactggtta cgagaccgac atgattgaaa gtgacatgga gcttgaaacc 6420 gagttgggaa tagatagcat caaacgtgtt gaaatcttgt ctgaggtcca agctatgttg 6480 aacgtggagg caaaagatgt cgatgcgctt tcaagaacca gaacagtcgg tgaggtcgtg 6540 gacgccatga aggctgaaat tgccggaagc tctgctagtg cgccagctgc agccgcacct 6600 gcacctgctg ccgcggcacc tgctccagct gcagccgctc ctgccgtcag caatgagttg 6660 ctggagaaag ccgaaacagt tgtgatggaa gtccttgctg caaaaactgg ttacgaaacg 6720 gacatgattg agtcagacat ggaattggag accgaacttg gaatagattc catcaagaga 6780 gtggagatcc tctctgaggt gcaagccatg ctcaacgttg aggctaaaga tgttgatgca 6840 ctcagtagga caagaactgt gggggaagtt gtcgatgcga tgaaggctga gattgctgga 6900 ggctctgctc ctgcgccggc agctgccgca cctgcacccg cagccgcggc accagctgtc 6960 agcaacgaat tgcttgagaa ggctgaaaca gttgtcatgg aagtgctggc tgcaaaaacc 7020 ggctatgaga ctgacatgat tgagtctgac atggaacttg agacggaatt gggcattgac 7080 agcatcaagc gtgttgagat cctcagtgaa gtccaagcaa tgctcaatgt ggaggctaag 7140 gatgtggatg ccctctcaag gaccagaaca gttggtgagg tcgttgatgc gatgaaggcg 7200 gagatcgctg gaagttccgc gcctgcgcca gctgcagccg ctcctgcacc cgcagccgcg 7260 gcaccggctc cagctgcagc cgcaccggcc gtctcctcag aactcctgga aaaggctgag 7320 acagttgtga tggaagtcct tgctgcgaaa actggctatg agaccgacat gattgaatct 7380 gacatggaac tggagaccga gcttggaatt gactccatca aacgtgttga aatcctctct 7440 gaggttcaag cgatgttgaa tgtggaggcc aaagatgttg atgctctttc cagaacaagg 7500 acggtgggag aggtggttga tgccatgaag gctgagatag ctgggggttc tgctcctgcg 7560 ccagctgcag ccgctcctgc tcccgctgcc gcggcaccag ctgtcagcaa cgaactcctt 7620 gaaaaagctg aaactgttgt gatggaggtt ttggctgcaa agactggata cgagactgac 7680 atgatagagt cagacatgga gttggaaaca gagcttggga ttgatagcat caagcgtgtg 7740 gaaatccttt ctgaggttca agccatgctg aacgttgagg ccaaagatgt cgatgctttg 7800 tcaaggacca gaacggttgg agaagtggtc gatgccatga aggctgagat agctggtgga 7860 tcagcccctg ctccggctgc agccgctcct gcaagtgccg gtgctgcacc agctgtgaag 7920 attgattcag ttcatggtgc tgactgtgac gatttgtcac tgatgcacgc aaaggtggtt 7980 gacatcagaa ggccagatga actgattctg gaaagaccgg agaaccgtcc agttcttgtc 8040 gttgacgatg gctcagagtt gacacttgct cttgtgaggg tgttgggagc ctgtgcagtt 8100 gtcttgacct ttgagggatt gcagttggct cagagagctg gtgcagctgc cattcgtcat 8160 gttctggcta aggacctttc agcagaatca gctgagaagg ccatcaaaga ggctgagcaa 8220 agatttggag cgttgggagg cttcataagc cagcaagcag aaaggtttga accagctgag 8280 atactgggtt tcacactcat gtgtgcgaag tttgccaaag cctctctctg cactgctgtg 8340 gctggaggtc gtcctgcctt catcggtgtg gctaggcttg acggcagact tggattcacc 8400 tctcaaggca cttctgacgc actcaagaga gcacagcgtg gagcgatctt tggactctgc 8460 aagacaatag ggttggagtg gtcagaatct gatgtgttca gccgtggtgt ggacattgct 8520 caagggatgc acccagaaga tgctgcagtt gccatagtta gggagatggc gtgtgctgac 8580 atcagaatca gagaggttgg gattggagca aaccagcaaa gatgcacaat cagagctgcc 8640 aagttggaga ctgggaaccc tcagagacag atagcgaaag acgatgtgct tctggtttct 8700 ggaggtgctc gtgggatcac acctctttgc atccgtgaga tcacgcgtca gattgctgga 8760 ggcaagtaca ttctgttggg tcgtagcaaa gtttctgcgt ctgaaccagc atggtgtgct 8820 ggcatcactg atgagaaggc tgtgcagaaa gctgccaccc aagagttgaa gagggccttc 8880 agtgctggtg aaggacccaa acccactcca agggctgtca cgaaacttgt gggatctgtt 8940 cttggtgctc gtgaagtgag aagctccatt gctgccattg aagctctggg tggcaaagcg 9000 atctacagct cttgtgatgt gaactctgct gcagatgttg ccaaagcagt tagggatgca 9060 gagtcccagc ttggtgccag agttagtgga atcgttcacg cttctggtgt gctccgtgat 9120 cgtctcatag agaagaaact ccctgacgag tttgatgctg tctttgggac caaagtcact 9180 gggttggaga atcttctggc tgcagttgat cgtgccaacc tcaaacacat ggttctcttc 9240 agctcccttg ctggtttcca cggcaatgtt ggtcagtctg actatgcgat ggcaaacgaa 9300 gctctcaaca agatgggttt ggaacttgcg aaggatgtca gtgtcaagtc catctgcttt 9360 ggtccctggg atggtgggat ggtgacacct caactcaaga aacagtttca agaaatggga 9420 gttcagatca ttcctcgtga aggaggtgct gatacagttg cgaggattgt gttgggatca 9480 agtcctgctg agatccttgt tggaaactgg aggacaccat ccaagaaagt tggaagtgac 9540 acgatcaccc tgcatagaaa gatcagtgcc aagtccaatc ccttcttgga ggatcatgtc 9600 atacaaggca gaagggtgtt gccaatgacc cttgctattg ggagccttgc tgagacctgc 9660 cttggactct tccctggcta ctctctctgg gccatagatg acgcacagct cttcaaaggt 9720 gtcactgttg atggagacgt caactgtgaa gtgaccctca caccgtccac tgctccgagt 9780 gggagggtga atgtccaagc gacgctcaag acgttctcaa gtggaaaact tgttccagcc 9840 tacagagctg tcatagtgtt gtccaaccaa ggtgctccac ctgccaatgc aaccatgcag 9900 cctccgagcc ttgatgcaga tcctgctttg caagggtcag tctatgatgg caagactctc 9960 tttcatggtc ctgcgttcag aggcattgat gacgttctca gctgcaccaa gagccagctt 10020 gttgccaagt gttctgctgt tcctggatca gatgcagcca gaggagagtt tgcaactgac 10080 actgatgcac atgatccctt tgtcaatgat cttgcctttc aagcaatgtt ggtctgggtg 10140 aggagaacgc tgggacaagc tgcactcccg aactccattc agaggatagt ccaacacaga 10200 ccggtcccac aagacaaacc cttctacatc accctcagat ccaatcagag tggtggacat 10260 agccagcaca aacatgcact tcagttccac aacgaacaag gtgatctctt cattgatgtt 10320 caagcctctg tcattgcgac agactcactt gcgttctgag tagttagctt aatcacttag 10380 gtcaccagta tgaactaaaa tgcatgtagg tgtaagagct catggagagc atggaatatt 10440 gtatccgacc atgtaacagt ataataactg agctccatct cacttcttct atgaataaac 10500 aaaggatgtt atgatatatt aacactctat ctatgcacct tattgttcta tgataaattt 10560 cctcttatta ttataaatca tctgaatcgt gacggcttat ggaatgcttc aaatagtaca 10620 aaaacaaatg tgtactataa gactttctaa acaattctaa ctttagcatt gtgaacgaga 10680 cataagtgtt aagaagacat aacaattata atggaagaag tttgtctcca tttatatatt 10740 atatattacc cacttatgta ttatattagg atgttaagga gacataacaa ttataaagag 10800 agaagtttgt atccatttat atattatata ctacccattt atatattata cttatccact 10860 tatttaatgt ctttataagg tttgatccat gatatttcta atattttagt tgatatgtat 10920 atgaaaaggt actatttgaa ctctcttact ctgtataaag gttggatcat ccttaaagtg 10980 ggtctattta attttattgc ttcttacaga taaaaaaaaa attatgagtt ggtttgataa 11040 aatattgaag gatttaaaat aataataaat aataaataac atataatata tgtatataaa 11100 tttattataa tataacattt atctataaaa aagtaaatat tgtcataaat ctatacaatc 11160 gtttagcctt gctggaacga atctcaatta tttaaacgag agtaaacata tttgactttt 11220 tggttattta acaaattatt atttaacact atatgaaatt tttttttttt atcagcaaag 11280 aataaaatta aattaagaag gacaatggtg tcccaatcct tatacaacca acttccacaa 11340 gaaagtcaag tcagagacaa caaaaaaaca agcaaaggaa attttttaat ttgagttgtc 11400 ttgtttgctg cataatttat gcagtaaaac actacacata acccttttag cagtagagca 11460 atggttgacc gtgtgcttag cttcttttat tttatttttt tatcagcaaa gaataaataa 11520 aataaaatga gacacttcag ggatgtttca acccttatac aaaaccccaa aaacaagttt 11580 cctagcaccc taccaacgaa ttcgcggccg cttaattaag atgagtgata ctcaggactc 11640 aggactcact ctgctgatca ctagtgctag cctcgaggtc gacacaactt tgtatacaaa 11700 agttgtctag acctaggctc agcaactcat ccagtcaagt tcgagtcagt gagttgggcg 11760 gccgcctccc agtatcatta tagtgaaagt tttggctctc tcgccggtgg ttttttacct 11820 ctatttaaag gggttttcca cctaaaaatt ctggtatcat tctcacttta cttgttactt 11880 taatttctca taatctttgg ttgaaattat cacgcttccg cacacgatat ccctacaaat 11940 ttattatttg ttaaacattt tcaaaccgca taaaatttta tgaagtcccg tctatcttta 12000 atgtagtcta acattttcat attgaaatat ataatttact taattttagc gttggtagaa 12060 agcataatga tttattctta ttcttcttca tataaatgtt taatatacaa tataaacaaa 12120 ttctttacct taagaaggat ttcccatttt atattttaaa aatatattta tcaaatattt 12180 ttcaaccacg taaatctcat aataataagt tgtttcaaaa gtaataaaat ttaactccat 12240 aattttttta ttcgactgat cttaaagcaa cacccagtga cacaactagc catttttttc 12300 tttgaataaa aaaatccaat tatcattgta ttttttttat acaatgaaaa tttcaccaaa 12360 caatgatttg tggtatttct gaagcaagtc atgttatgca aaattctata attcccattt 12420 gacactacgg aagtaactga agatctgctt ttacatgcga gacacatctt ctaaagtaat 12480 tttaataata gttactatat tcaagatttc atatatcaaa tactcaatat tacttctaaa 12540 aaattaatta gatataatta aaatattact tttttaattt taagtttaat tgttgaattt 12600 gtgactattg atttattatt ctactatgtt taaattgttt tatagatagt ttaaagtaaa 12660 tataagtaat gtagtagagt gttagagtgt taccctaaac cataaactat aagatttatg 12720 gtggactaat tttcatatat ttcttattgc ttttaccttt tcttggtatg taagtccgta 12780 actggaatta ctgtgggttg ccatgacact ctgtggtctt ttggttcatg catggatctt 12840 gcgcaagaaa aagacaaaga acaaagaaaa aagacaaaac agagagacaa aacgcaatca 12900 cacaaccaac tcaaattagt cactggctga tcaagatcgc cgcgtccatg tatgtctaaa 12960 tgccatgcaa agcaacacgt gcttaacatg cactttaaat ggctcaccca tctcaaccca 13020 cacacaaaca cattgccttt ttcttcatca tcaccacaac cacctgtata tattcattct 13080 cttccgccac ctcaatttct tcacttcaac acacgtcaac ctgcatatgc gtgtcatccc 13140 atgcccaaat ctccatgcat gttccaacca ccttctctct tatataatac ctataaatac 13200 ctctaatatc actcacttct ttcatcatcc atccatccag agtactacta ctctactact 13260 ataatacccc aacccaactc atattcaata ctactctagg atccacaacc aaaaatggct 13320 gcaaggaacg tgagtgcggc tcatgagatg catgacgaga agaggatagc tgttgtgggc 13380 atggctgttc agtatgctgg atgcaaaacg aaagacgagt tctgggaagt tctcatgaat 13440 ggaaaagttg agagcaaagt gatcagcgac aaacgtcttg gttcaaatta ccgtgctgaa 13500 cactacaaag ctgaaaggag caagtatgct gacaccttct gcaatgagac atatggaact 13560 cttgatgaga acgaaataga caatgagcat gaactccttt tgaaccttgc caaacaagcc 13620 ttggctgaaa catctgtcaa agattcaaca agatgtggca tagtttctgg atgcctcagc 13680 ttcccgatgg acaacctcca aggtgaactc ttgaatgtgt accagaacca tgttgagaag 13740 aaacttggtg ccagagtttt caaagatgcc tctcactggt cagaaagaga acagtccaac 13800 aaaccagagg ctggtgacag aagaatcttc atggaccctg cttcctttgt tgctgaggaa 13860 ctcaatcttg gcgctctcca ctactctgtt gatgctgcct gtgccactgc gctctatgtg 13920 ctcagactgg cgcaagatca cttggtctct ggtgcagctg atgtgatgct ttgtggtgca 13980 acatgcctgc cggagccgtt tttcatactc tctgggttct caaccttcca agccatgcct 14040 gttggaacgg gtcagaatgt gtccatgccg ctgcacaaag attctcaagg attgacccct 14100 ggtgaaggag gttccatcat ggtcctcaaa cgtttggatg acgcaatcag agatggagat 14160 cacatctatg gaacactttt gggagccaat gtcagcaaca gtggcactgg tctgccactt 14220 aaaccccttt tgccgtcaga gaaaaagtgc ctcatggaca cctacacacg tatcaatgtg 14280 catccccaca agattcagta tgttgaatgc catgcaactg ggactccaca aggtgatcgt 14340 gtggaaattg atgctgtgaa ggcttgcttt gaaggcaaag tcccacgttt tggaaccaca 14400 aaagggaact ttggacacac attggtggca gctgggtttg ctggcatgtg caaagtgctt 14460 ctctccatga agcacgggat cattccaccc actccgggaa tagatgacga aacgaagatg 14520 gaccctctgg ttgtctctgg tgaggccatt ccctggccag aaacgaatgg agaaccgaag 14580 cgtgctggtc tctcagcgtt tggatttggt gggaccaatg ctcatgctgt ctttgaggaa 14640 catgacccaa gcaatgctgc atgcactggc catgactcca tttctgctct ctctgcacgt 14700 tgtggtggtg agtcaaacat gagaatagca atcactggga tggatgccac ttttggtgct 14760 ttgaaaggat tggatgcttt tgaaagggcc atctacactg gtgctcatgg tgctatacca 14820 ctcccagaaa agagatggag attcctgggg aaagacaagg acttccttga tctttgtgga 14880 gtcaaagcaa caccgcatgg ctgttacatt gaagatgttg aagttgattt ccagaggctg 14940 aggactccaa tgactcctga agatatgctc ttgcctcaac agcttctggc tgtcaccaca 15000 attgataggg ccatattgga ctctggcatg aagaaaggtg gaaatgtggc tgtctttgtg 15060 gggttgggga ccgatcttga actctaccgt caccgtgcaa gggttgctct caaggagaga 15120 gtgaggcctg aagccagcaa gaaactcaat gacatgatgc agtacatcaa tgattgtggg 15180 acaagcactt cttacaccag ctacataggc aacttggttg caacaagggt cagttcacaa 15240 tggggcttca cgggaccttc cttcacaatc acagaaggca acaattctgt gtacagatgt 15300 gctgaacttg gcaaatacct cttggaaacg ggagaggttg atggggttgt ggttgcgggt 15360 gttgatctct gtgggagtgc tgagaacctt tacgtcaagt caaggcgttt caaggtgtct 15420 acatctgaca ccccgagagc cagctttgat gcagctgctg atggttactt tgttggagag 15480 ggctgtggtg catttgtgct caagcgtgag acaagctgca ccaaggatga cagaatctat 15540 gcttgcatgg atgccatagt tcctggaaat gttccatctg catgtttgag agaggcattg 15600 gatcaagcca gagtcaagcc tggagacatc gagatgttgg agctttctgc tgattcagcg 15660 aggcatttga aggacccgtc tgttctccca aaggaactca ctgctgagga agagattggc 15720 ggacttcaga cgattcttcg tgacgatgac aagctcccaa ggaatgttgc aactggaagt 15780 gtcaaggcca ctgttggtga cactggttat gcttctggtg ctgccagttt gattaaggct 15840 gcgctttgca tctacaaccg ttacctgccc agcaatggag atgactggga tgaacctgca 15900 cctgaagcac cctgggacag cacactcttt gcgtgccaga cctcaagagc ttggttgaag 15960 aaccctggtg agagacgtta tgcggctgtt tccggagtca gtgaaaccag aagttgctac 16020 agtgtgctgc tttctgaagc agagggacac tacgagagag agaacagaat cagtttggat 16080 gaggaagcac ccaagctcat tgtgttgaga gcagactccc atgaagagat tcttggaaga 16140 ttggacaaga taagggaaag gttcttgcag cccacgggtg ctgcacccag agagtctgaa 16200 ttgaaggcgc aagcccgtag gatctttctt gaactccttg gtgagaccct tgcccaagat 16260 gcagcttcat ctggaagtca gaagcccctt gctctcagct tggtctccac tccatccaag 16320 ctccagagag aggtggaact tgcggcaaaa ggcattccga gatgtctcaa gatgagacgt 16380 gattggagtt cccctgctgg aagcagatat gcgcctgagc ctttggccag tgacagagtt 16440 gccttcatgt atggagaagg aaggagccct tactatggca tcacacaaga cattcatagg 16500 atttggcctg aacttcatga ggtcatcaat gagaaaacaa accgtctctg ggctgaaggt 16560 gacagatggg tcatgccaag ggccagcttc aagagtgagc ttgagtcaca acagcaagag 16620 tttgacagaa acatgattga aatgttccgt cttggaatcc tcacctcaat tgccttcaca 16680 aacctggcga gagatgttct caacatcacg cccaaagcag cctttggact cagtttggga 16740 gagatttcca tgatctttgc tttctccaag aaaaacggtt tgataagcga tcagttgaca 16800 aaggatttga gagagtcaga tgtctggaac aaagctctgg ctgttgagtt caatgcgctc 16860 agagaagcct ggggcattcc acagtctgtc ccaaaagatg agttctggca aggttacata 16920 gtgaggggaa cgaaacaaga catagaagca gccatagcac cagactcaaa gtatgtgagg 16980 ttgaccatca tcaatgatgc caacactgct ctcatcagtg gaaagcctga tgcatgcaaa 17040 gctgcgattg ctcgtcttgg tggcaacatc cctgcgcttc cagtcactca aggcatgtgt 17100 gggcattgcc cagaggtggg accttacacc aaggacatcg caaagatcca cgccaacctt 17160 gagttccctg tggttgatgg tcttgatctc tggactacca taaaccagaa gaggcttgtg 17220 ccaagagcca cgggtgccaa agatgaatgg gctccttcat cctttgggga gtatgctgga 17280 cagctctatg agaaacaagc caacttccct cagatagttg aaaccatcta caagcagaac 17340 tatgatgtgt ttgttgaagt tgggccaaac aatcacagaa gcacagcagt gagaaccacg 17400 cttggaccac agagaaacca ccttgctggt gccatagaca agcagaacga ggatgcttgg 17460 acgacaatag tcaagctcgt ggcttcattg aaggctcacc ttgttcctgg agtgaccatc 17520 agtccgctct accactccaa gctcgttgca gaggctgagg cttgttatgc tgcgctctgt 17580 aaaggtgaga aacccaagaa aaacaagttt gtgagaaaga ttcagttgaa tggcagattc 17640 aacagcaagg cagaccccat ctcctcagca gaccttgcat ccttcccacc tgcggaccct 17700 gcgattgaag ctgccatctc ttcaaggatc atgaagcctg tggctccgaa attctatgcg 17760 cgtctcaaca ttgatgagca agatgagacc agagatccga tcttgaacaa ggacaatgcg 17820 ccttccagct ccagctcttc cagcagttcc tcttccagca gttcatctcc gtctcctgct 17880 ccttcagctc cagttcagaa gaaagctgcc ccagctgcgg agacaaaagc tgttgccagt 17940 gctgatgcac ttcgtagtgc cctgcttgat cttgactcca tgcttgcgct cagctctgca 18000 agtgcttctg gcaaccttgt tgagactgcg ccatctgatg ccagtgtcat tgtccctccg 18060 tgcaacattg ctgatcttgg cagcagagct ttcatgaaaa cttatggtgt ttctgcgcct 18120 ctgtacaccg gtgcaatggc caagggaatt gcctctgcgg acttggtgat cgcagctgga 18180 aggcaaggaa tccttgcgtc atttggtgct ggaggccttc caatgcaagt tgtgcgtgag 18240 tccattgaaa agattcaagc tgccctgccc aatggaccat atgctgtcaa cctcatccat 18300 tctccttttg acagcaacct tgagaaggga aatgttgatc tcttcttgga aaagggtgtc 18360 acttttgttg aggcttcagc cttcatgacg ttgacaccgc aagttgtcag atacagagcg 18420 gctgggttga cgaggaatgc agatgggtca gtcaacataa ggaacagaat cattgggaaa 18480 gtctccagaa ctgagctggc tgaaatgttc atgcgtcctg cacctgagca tcttttgcag 18540 aagctcattg cttctgggga gatcaatcaa gaacaagctg agttggccag acgtgttcct 18600 gtggctgatg acattgcggt tgaagctgat tctggtggac acactgacaa ccgtccaatc 18660 catgtcattc tgcctctgat tatcaacctg agggataggc ttcacagaga gtgtggatac 18720 ccagcaaacc tgagggttcg tgttggtgct gggggtggca ttgggtgtcc ccaagctgcg 18780 ctggcaactt tcaacatggg tgcctctttc attgtcactg gaacagtgaa ccaagttgcc 18840 aaacaatctg ggacgtgtga caatgtgagg aaacagcttg cgaaagcaac ttactcagat 18900 gtttgcatgg caccggcagc tgacatgttt gaggaaggtg tgaaacttca agttttgaag 18960 aaaggaacaa tgttcccttc aagagccaac aaactctatg agttgttctg caagtatgat 19020 tcttttgagt ccatgcctcc agcagagctt gcgagggttg agaaaagaat cttcagtcgt 19080 gcattggaag aggtctggga tgagaccaag aacttctaca tcaaccgtct ccacaaccca 19140 gagaagatac agagggctga gagggacccc aaactgaaga tgtcactgtg tttcagatgg 19200 tatctgtccc tggcttcaag gtgggccaac actggagctt ctgacagagt catggactac 19260 caagtttggt gtggtcctgc cattggttca ttcaatgatt tcatcaaagg aacttacctt 19320 gatccggcag tggcaaatga gtacccgtgt gttgtgcaga tcaacaaaca gattcttaga 19380 ggagcgtgct tcttgaggcg tcttgaaata ctgagaaatg caaggctttc agatggtgca 19440 gctgcacttg tggcgagcat tgatgacaca tatgtccctg ctgagaagct gtgagtagtt 19500 agcttaatca cttaggtcac cagtatgaac taaaatgcat gtaggtgtaa gagctcatgg 19560 agagcatgga atattgtatc cgaccatgta acagtataat aactgagctc catctcactt 19620 cttctatgaa taaacaaagg atgttatgat atattaacac tctatctatg caccttattg 19680 ttctatgata aatttcctct tattattata aatcatctga atcgtgacgg cttatggaat 19740 gcttcaaata gtacaaaaac aaatgtgtac tataagactt tctaaacaat tctaacttta 19800 gcattgtgaa cgagacataa gtgttaagaa gacataacaa ttataatgga agaagtttgt 19860 ctccatttat atattatata ttacccactt atgtattata ttaggatgtt aaggagacat 19920 aacaattata aagagagaag tttgtatcca tttatatatt atatactacc catttatata 19980 ttatacttat ccacttattt aatgtcttta taaggtttga tccatgatat ttctaatatt 20040 ttagttgata tgtatatgaa aaggtactat ttgaactctc ttactctgta taaaggttgg 20100 atcatcctta aagtgggtct atttaatttt attgcttctt acagataaaa aaaaaattat 20160 gagttggttt gataaaatat tgaaggattt aaaataataa taaataataa ataacatata 20220 atatatgtat ataaatttat tataatataa catttatcta taaaaaagta aatattgtca 20280 taaatctata caatcgttta gccttgctgg aacgaatctc aattatttaa acgagagtaa 20340 acatatttga ctttttggtt atttaacaaa ttattattta acactatatg aaattttttt 20400 tttttatcag caaagaataa aattaaatta agaaggacaa tggtgtccca atccttatac 20460 aaccaacttc cacaagaaag tcaagtcaga gacaacaaaa aaacaagcaa aggaaatttt 20520 ttaatttgag ttgtcttgtt tgctgcataa tttatgcagt aaaacactac acataaccct 20580 tttagcagta gagcaatggt tgaccgtgtg cttagcttct tttattttat ttttttatca 20640 gcaaagaata aataaaataa aatgagacac ttcagggatg tttcaaccct tatacaaaac 20700 cccaaaaaca agtttcctag caccctacca acgaattcgc ggccgcttaa ttaatctgac 20760 ctgaccacta gacctgagtg agtacagatg tgctactagt gctagcctcg aggtcgacca 20820 acttttctat acaaagttgt ctagacctag gactaggcac tgagtgactc actgacctga 20880 ctgagtgact ggcggccgcc tcccagtatc attatagtga aagttttggc tctctcgccg 20940 gtggtttttt acctctattt aaaggggttt tccacctaaa aattctggta tcattctcac 21000 tttacttgtt actttaattt ctcataatct ttggttgaaa ttatcacgct tccgcacacg 21060 atatccctac aaatttatta tttgttaaac attttcaaac cgcataaaat tttatgaagt 21120 cccgtctatc tttaatgtag tctaacattt tcatattgaa atatataatt tacttaattt 21180 tagcgttggt agaaagcata atgatttatt cttattcttc ttcatataaa tgtttaatat 21240 acaatataaa caaattcttt accttaagaa ggatttccca ttttatattt taaaaatata 21300 tttatcaaat atttttcaac cacgtaaatc tcataataat aagttgtttc aaaagtaata 21360 aaatttaact ccataatttt tttattcgac tgatcttaaa gcaacaccca gtgacacaac 21420 tagccatttt tttctttgaa taaaaaaatc caattatcat tgtatttttt ttatacaatg 21480 aaaatttcac caaacaatga tttgtggtat ttctgaagca agtcatgtta tgcaaaattc 21540 tataattccc atttgacact acggaagtaa ctgaagatct gcttttacat gcgagacaca 21600 tcttctaaag taattttaat aatagttact atattcaaga tttcatatat caaatactca 21660 atattacttc taaaaaatta attagatata attaaaatat tactttttta attttaagtt 21720 taattgttga atttgtgact attgatttat tattctacta tgtttaaatt gttttataga 21780 tagtttaaag taaatataag taatgtagta gagtgttaga gtgttaccct aaaccataaa 21840 ctataagatt tatggtggac taattttcat atatttctta ttgcttttac cttttcttgg 21900 tatgtaagtc cgtaactgga attactgtgg gttgccatga cactctgtgg tcttttggtt 21960 catgcatgga tcttgcgcaa gaaaaagaca aagaacaaag aaaaaagaca aaacagagag 22020 acaaaacgca atcacacaac caactcaaat tagtcactgg ctgatcaaga tcgccgcgtc 22080 catgtatgtc taaatgccat gcaaagcaac acgtgcttaa catgcacttt aaatggctca 22140 cccatctcaa cccacacaca aacacattgc ctttttcttc atcatcacca caaccacctg 22200 tatatattca ttctcttccg ccacctcaat ttcttcactt caacacacgt caacctgcat 22260 atgcgtgtca tcccatgccc aaatctccat gcatgttcca accaccttct ctcttatata 22320 atacctataa atacctctaa tatcactcac ttctttcatc atccatccat ccagagtact 22380 actactctac tactataata ccccaaccca actcatattc aatactactc taggatccac 22440 aaccaaaaat ggcgcttcgt gtcaagacca acaagaagcc atgttgggag atgaccaagg 22500 aggaactgac ctctggcaaa actgaggtgt tcaactatga ggaactcctg gagtttgcag 22560 agggtgacat agctaaggtt tttggtccgg agtttgctgt catagacaag taccccagaa 22620 gggtgaggct gcctgctcgt gaatacctgc ttgtgacaag ggtcacactc atggatgccg 22680 aggtcaacaa ctacagagtt ggtgccagaa tggtcactga gtatgatctt ccagtgaatg 22740 gcgaattgtc tgagggaggt gattgtccct gggcagttct ggttgagagt ggacagtgcg 22800 atttgatgct gatatcatac atgggaattg atttccagaa ccaaggtgac agagtttacc 22860 gtctgctcaa caccacgttg acattctatg gggttgccca tgagggagag acccttgagt 22920 atgacatcag agtgaccggt tttgccaaac gtttggatgg tggcatctcc atgttcttct 22980 ttgagtatga ctgctatgtc aatgggaggc ttctcattga gatgagagat ggctgtgctg 23040 gcttcttcac aaatgaggag cttgatgcgg gaaagggtgt ggtgttcaca agaggggatt 23100 tggctgccag agctaagatt ccaaagcaag atgtttctcc ctatgctgtc gctccctgcc 23160 ttcacaaaac aaaactcaac gaaaaggaga tgcagacctt ggttgacaaa gattgggctt 23220 ccgtttttgg ctcaaagaat ggcatgccgg agatcaacta caaactctgc gcacgtaaga 23280 tgctcatgat tgatagagtc accagcattg accataaggg aggtgtgtac ggtttgggtc 23340 agttggttgg tgagaaaatc ttggagaggg accattggta cttcccatgc cattttgtta 23400 aggatcaagt catggctgga tctcttgtca gcgacggttg ttcacaaatg ctcaagatgt 23460 acatgatctg gctcggtttg caccttacca ccggaccatt tgacttcaga cccgtcaacg 23520 gacacccgaa caaagttagg tgtaggggtc agatatctcc tcataaggga aagttggtct 23580 acgtcatgga gatcaaagag atgggattcg acgaggataa cgatccgtac gccattgctg 23640 acgtcaacat cattgacgtt gatttcgaaa agggtcaaga tttcagcctt gatagaatca 23700 gcgactacgg aaagggggat cttaacaaaa agatcgtcgt ggacttcaag ggcattgctc 23760 tcaagatgca gaagagatcc acgaacaaga acccctccaa agttcagccc gtttttgcta 23820 acggtgcagc cactgtggga ccggaagcgt ccaaggccag ctctggtgcc agtgctagtg 23880 cttctgctgc ccctgcgaag cctgctttct cagcagatgt tcttgcgcca aagccagttg 23940 cacttcctga acatatcctc aagggtgacg ctctcgctcc gaaagaaatg tcctggcatc 24000 ccatgtcaaa acttcctggc aacccgacgc cgttcttctc ccctagttct taccctccga 24060 gggcaatttg cttcatccct ttccctggca acccacttga caacaattgc aaagctggag 24120 aaatgcctct gaactggtac aacatgtcag agttcatgtg tggaaaagtt agcaactgct 24180 tgggaccaga gttcgcaaga tttgataagt caaatacgag cagaagccct gcttttgatt 24240 tggctcttgt gactagagtt gttgaagtca caaacatgga acatggcaag ttcctgaatg 24300 ttgattgcaa tcccagcaaa ggcacaatgg tgggggagtt tgactgtcct caagatgcgt 24360 ggttctttga tggttcttgc aatgatgggc acatgcctta ttccatcatc atggaaatcg 24420 gacttcagac atccggtgtt ctcaccagtg tgttgaaagc accactgacg atggacaaag 24480 atgacattct ctttaggaac cttgatgcaa gtgctgaaat ggtgaggcca gatgtggatg 24540 tgagggggaa aaccataagg aatgtgacca agtgcactgg atatgcaatg ttgggaaaga 24600 tggggattca cagattcacg tttgagttgt ctgttgatgg cgtggttttc tacaaaggaa 24660 gcacttcctt tggatggttc actccagagg tgtttgctca gcaagctgga cttgacaatg 24720 ggaaaaagac ggagccttgg tgcaaaacta acaacacgag tgtgagaaga gttgaaatcg 24780 catcagccaa aggcaaagaa cagctgactg agaaacttcc agatgcaact aatgctcaag 24840 ttctcagaag gtcagaacag tgtgagtatt tggattacct caacattgct cctgattctg 24900 ggctgcatgg gaaaggatat gcgcatggac acaaagatgt taacccacaa gactggttct 24960 tctcttgtca cttttggttt gatcctgtga tgcctggatc tttgggaatt gagtcaatgt 25020 tccagctcat tgaggcattt gcggtggacc agaacatacc gggagaatac aatgtgagca 25080 atccgacctt tgcccatgcc cctggaaaaa cggcgtggaa gtaccgtggt caattgacac 25140 caaagaaccg tgcgatggac tgtgaggtgc acatagtttc aatcacagcc tctcctgaaa 25200 atggaggcta cgttgacata gtggcagatg gagcactttg ggttgatggt ctccgtgtgt 25260 atgaagccaa agaactcaga gttcgtgttg tcagtgcaaa acctcaagcc attccggatg 25320 ttcaacaaca gccacccagc gcaaaagcgg accctgggaa aactggagtt gcactcagtc 25380 cgactcagtt gagagatgtc ctgcttgaag tggacaatcc attgtatctt ggtgttgaaa 25440 actccaattt ggtgcagttt gaatccaaac ctgcaacttc ttcaaggatt gtcagtatca 25500 aaccatgctc cataagtgat cttggtgaca agtccttcat ggaaacgtac aatgtgtcag 25560 cacctctgta cactggagca atggccaagg gaattgcgtc tgcggacctt gtcattgctg 25620 ctgggaagag gaagatattg ggttcttttg gagcgggtgg gcttccaatg caccatgtga 25680 gggcagcttt ggagaagata caagcggctc tgcctcaagg accatatgct gtgaacctca 25740 ttcattcacc ttttgacagc aacttggaaa aggggaatgt tgatctcttc ctcgaaaagg 25800 gcgttactgt ggtggaggct tctgcattca tgaccctcac tccccaagtt gttcgttacc 25860 gtgcagccgg actctcaagg aacgctgatg ggagtgtcaa catcagaaac cgtatcattg 25920 gaaaagtgtc cagaacagaa ctcgccgaaa tgttcatcag accggctccg gaacacctcc 25980 ttgagaaact catagcttct ggtgagataa cacaagaaca agccgaactt gcccgtcgtg 26040 ttcctgtcgc agacgacatc gctgttgaag ctgattctgg tggtcacact gacaaccgtc 26100 ccatccacgt catcttgcct ttgatcatca accttaggaa cagactgcac agagagtgtg 26160 gttatcctgc gcaccttaga gtcagagttg gagctggagg cggtgttggc tgcccacaag 26220 ctgctgcggc tgccctcaca atgggagctg ccttcatagt gactgggact gtcaatcaag 26280 ttgctaaaca gtccgggaca tgtgacaacg tgagaaaaca actcagtcaa gccacctaca 26340 gtgacatttg catggctcca gcagctgata tgtttgagga aggtgtgaaa ctccaagttc 26400 tcaagaaggg aacaatgttc ccatcaagag ctaacaaatt gtacgaactc ttttgcaagt 26460 acgactcttt cgattcaatg cctcctgctg aacttgaaag gatcgagaag aggatcttca 26520 agagggcact tcaagaggtc tgggaagaga ccaaggactt ttacatcaat ggtttgaaga 26580 atccagagaa gatacagaga gcggaacatg accccaaact caagatgtct ttgtgtttca 26640 gatggtatct tggtcttgca tcacgttggg ccaacatggg tgctcctgat cgtgtcatgg 26700 actatcaagt ctggtgtggt ccagccattg gagccttcaa tgacttcatc aaaggaacat 26760 atcttgatcc tgctgtctct aatgagtacc catgtgttgt ccagatcaac cttcagattt 26820 tgcgtggtgc atgttacctg aggcgtctta atgctctgag aaatgatccg aggattgatc 26880 ttgagactga agatgctgcc tttgtctatg agccaacgaa tgcgctttga gtagttagct 26940 taatcactta ggtcaccagt atgaactaaa atgcatgtag gtgtaagagc tcatggagag 27000 catggaatat tgtatccgac catgtaacag tataataact gagctccatc tcacttcttc 27060 tatgaataaa caaaggatgt tatgatatat taacactcta tctatgcacc ttattgttct 27120 atgataaatt tcctcttatt attataaatc atctgaatcg tgacggctta tggaatgctt 27180 caaatagtac aaaaacaaat gtgtactata agactttcta aacaattcta actttagcat 27240 tgtgaacgag acataagtgt taagaagaca taacaattat aatggaagaa gtttgtctcc 27300 atttatatat tatatattac ccacttatgt attatattag gatgttaagg agacataaca 27360 attataaaga gagaagtttg tatccattta tatattatat actacccatt tatatattat 27420 acttatccac ttatttaatg tctttataag gtttgatcca tgatatttct aatattttag 27480 ttgatatgta tatgaaaagg tactatttga actctcttac tctgtataaa ggttggatca 27540 tccttaaagt gggtctattt aattttattg cttcttacag ataaaaaaaa aattatgagt 27600 tggtttgata aaatattgaa ggatttaaaa taataataaa taataaataa catataatat 27660 atgtatataa atttattata atataacatt tatctataaa aaagtaaata ttgtcataaa 27720 tctatacaat cgtttagcct tgctggaacg aatctcaatt atttaaacga gagtaaacat 27780 atttgacttt ttggttattt aacaaattat tatttaacac tatatgaaat tttttttttt 27840 tatcagcaaa gaataaaatt aaattaagaa ggacaatggt gtcccaatcc ttatacaacc 27900 aacttccaca agaaagtcaa gtcagagaca acaaaaaaac aagcaaagga aattttttaa 27960 tttgagttgt cttgtttgct gcataattta tgcagtaaaa cactacacat aaccctttta 28020 gcagtagagc aatggttgac cgtgtgctta gcttctttta ttttattttt ttatcagcaa 28080 agaataaata aaataaaatg agacacttca gggatgtttc aacccttata caaaacccca 28140 aaaacaagtt tcctagcacc ctaccaacga attcgcggcc gcttaattaa tcacgagtgg 28200 atgactgtca cgactgactc atgactgact actagtgcta gcctcgaggt cgacacaact 28260 ttgtataata aagttgtcta gacctaggcg agtcatgagt cgtgacatga cgtcatgctc 28320 atcagactgc ggccgcctcc cagtatcatt atagtgaaag ttttggctct ctcgccggtg 28380 gttttttacc tctatttaaa ggggttttcc acctaaaaat tctggtatca ttctcacttt 28440 acttgttact ttaatttctc ataatctttg gttgaaatta tcacgcttcc gcacacgata 28500 tccctacaaa tttattattt gttaaacatt ttcaaaccgc ataaaatttt atgaagtccc 28560 gtctatcttt aatgtagtct aacattttca tattgaaata tataatttac ttaattttag 28620 cgttggtaga aagcataatg atttattctt attcttcttc atataaatgt ttaatataca 28680 atataaacaa attctttacc ttaagaagga tttcccattt tatattttaa aaatatattt 28740 atcaaatatt tttcaaccac gtaaatctca taataataag ttgtttcaaa agtaataaaa 28800 tttaactcca taattttttt attcgactga tcttaaagca acacccagtg acacaactag 28860 ccattttttt ctttgaataa aaaaatccaa ttatcattgt atttttttta tacaatgaaa 28920 atttcaccaa acaatgattt gtggtatttc tgaagcaagt catgttatgc aaaattctat 28980 aattcccatt tgacactacg gaagtaactg aagatctgct tttacatgcg agacacatct 29040 tctaaagtaa ttttaataat agttactata ttcaagattt catatatcaa atactcaata 29100 ttacttctaa aaaattaatt agatataatt aaaatattac ttttttaatt ttaagtttaa 29160 ttgttgaatt tgtgactatt gatttattat tctactatgt ttaaattgtt ttatagatag 29220 tttaaagtaa atataagtaa tgtagtagag tgttagagtg ttaccctaaa ccataaacta 29280 taagatttat ggtggactaa ttttcatata tttcttattg cttttacctt ttcttggtat 29340 gtaagtccgt aactggaatt actgtgggtt gccatgacac tctgtggtct tttggttcat 29400 gcatggatct tgcgcaagaa aaagacaaag aacaaagaaa aaagacaaaa cagagagaca 29460 aaacgcaatc acacaaccaa ctcaaattag tcactggctg atcaagatcg ccgcgtccat 29520 gtatgtctaa atgccatgca aagcaacacg tgcttaacat gcactttaaa tggctcaccc 29580 atctcaaccc acacacaaac acattgcctt tttcttcatc atcaccacaa ccacctgtat 29640 atattcattc tcttccgcca cctcaatttc ttcacttcaa cacacgtcaa cctgcatatg 29700 cgtgtcatcc catgcccaaa tctccatgca tgttccaacc accttctctc ttatataata 29760 cctataaata cctctaatat cactcacttc tttcatcatc catccatcca gagtactact 29820 actctactac tataataccc caacccaact catattcaat actactctag gatccacaac 29880 caaaaatgcc cactccagat gccactgcgc ctctcaacaa gccgtcagac tatgctgtgt 29940 accacgaaga ggatggtcct ttctggactg ctgattcctc tggtgtcagc agagtgaact 30000 tctctgaaac tggtgtggga tctgaaggtg tcattcctgc gctcacactg attgatgtgt 30060 ttgagcgtgc ggtcaaacgt ggtggcaata ggattgcatt cagaacagag aacatgccaa 30120 ccttgaggcg tggagaagag gctcctgatg ctttgccgct caaagattgg aagtcctggt 30180 cttggaaaca gtacaaagct gatgttcaca gaattgcgag ggctctgatg gatttgggag 30240 ttgagcagca cgatgcggtc tccatctttg gcttcaactc accagaatgg ttcctcagtg 30300 ctgtcggtgc agttcatgct ggtgccaaga tcgctggaat ctacccttca gacactcctg 30360 ctcaagtgca gtacaaagcc ttccactctg acactgctgt tgcagttgtg gagaacgaac 30420 agtgcttcaa gaaattcgca gaggtggttg aggatctccc ttacctcaaa gccattgttt 30480 gttgggatta tgaagccaca gacatcacaa gggaagatgg ttcagtggtt gaagtcctga 30540 cctttgctga gttcctgaag agaggtgaca ctgtggaagc agctgcactt gatgagagaa 30600 tcagcaagat tgagcccacc atgtgtgctg ccctgatcta caccagtggc acaactggga 30660 gacccaaagc tgtcatgatc tcccatgaca accttgtctt tgaagcatct gctgtggtcc 30720 cgaacttggg aggggcatgc acgaccactg ctgaggaaag gattctttcc taccttcctt 30780 tgtctcatgt tgctggaatg atggttgaca tcattgctcc aatcatagcg acagcgttcc 30840 acaaaggaag gatctgtgtt tgttttgcca gaccgtatga tttgagaact ggaacccttg 30900 ggcaaagact caatgcagtg gagccaacca tattccttgg tgtgccacgt gtgtgggaaa 30960 agatccaaga gaaattgatg gctgttggtg ccaaaaccac tggtctcaag aagaaactca 31020 gcacggcagc caagaaacgt gggcttgaat ttcaagagga acaacagata gggagaagtg 31080 gtgccaaccc tggctttgga ccgcttggaa tctacaagaa acttttgggc ctcatcaaag 31140 gaaagttggg gctgaccaaa tgcaagtttg cctttgctgg cgcagctcca atgacccgtg 31200 aaacccttca gtactttgga gcgctgaaca tcaacatcaa tgaggtctat ggaatgtctg 31260 aatgcagtgg ggctgccacc tggtcaactg acaaagctca tgagtggggc actgttggct 31320 atgagatgcc ctcctgtgaa gtcagagtgt tcaagattgc ggaggatggc accaagacag 31380 agtgcccaag agctgctgac atcatgcatg ccacagaaga ggaacaaggt gaagtttgct 31440 tccgtggaag gaacatcatg atgggatact tggccaaccc gaaacttggt gatgaccatg 31500 ttgctgagat tgaagagaag aatgcagctg ccatagatag cgaagggtgg cttcactctg 31560 gtgacaaggg agcaatttcc acaaggggaa tgctcaagat aacgggcaga tacaaggagc 31620 ttatcatagg tgctggtggg gagaatgtgg ctcctgttcc cattgaagat gccatcaagg 31680 cgaggatgcc ttttgtttca aatgcaatga tggttggaga caagaggaag ttcatggctg 31740 tccttctgac tttgaaaacg gttggagcaa ctggagagct tcctggcaca aacaagctca 31800 tgggagcagc tgcggactat ggtgagacca ttgaggatgc ctgtgacaat gaggcgctca 31860 ttgaagagat aacgcagcaa ctcaaagaga taggtgacga tggagatgtc acgccaagca 31920 atgcagcccg tatacaaaag ttcacgattt tgccactcga cttcagtgtc tccacagatg 31980 aactgacagc aacgttgaaa ttgaagagaa gtgtggttgc agacaagtat gaggacatca 32040 tagaggcttt ctatgagagc aagtctgtct ttgtgcctta ctcaacagtt ggagcttatg 32100 caacgggtgg accagttgat gactctgttg tggatggatc attcaaaggt gactttagca 32160 tgattgggga tgacgatccg gatcttcaga atgtggatgt ccttgaagca attgatgagg 32220 acaactgagt agttagctta atcacttagg tcaccagtat gaactaaaat gcatgtaggt 32280 gtaagagctc atggagagca tggaatattg tatccgacca tgtaacagta taataactga 32340 gctccatctc acttcttcta tgaataaaca aaggatgtta tgatatatta acactctatc 32400 tatgcacctt attgttctat gataaatttc ctcttattat tataaatcat ctgaatcgtg 32460 acggcttatg gaatgcttca aatagtacaa aaacaaatgt gtactataag actttctaaa 32520 caattctaac tttagcattg tgaacgagac ataagtgtta agaagacata acaattataa 32580 tggaagaagt ttgtctccat ttatatatta tatattaccc acttatgtat tatattagga 32640 tgttaaggag acataacaat tataaagaga gaagtttgta tccatttata tattatatac 32700 tacccattta tatattatac ttatccactt atttaatgtc tttataaggt ttgatccatg 32760 atatttctaa tattttagtt gatatgtata tgaaaaggta ctatttgaac tctcttactc 32820 tgtataaagg ttggatcatc cttaaagtgg gtctatttaa ttttattgct tcttacagat 32880 aaaaaaaaaa ttatgagttg gtttgataaa atattgaagg atttaaaata ataataaata 32940 ataaataaca tataatatat gtatataaat ttattataat ataacattta tctataaaaa 33000 agtaaatatt gtcataaatc tatacaatcg tttagccttg ctggaacgaa tctcaattat 33060 ttaaacgaga gtaaacatat ttgacttttt ggttatttaa caaattatta tttaacacta 33120 tatgaaattt ttttttttta tcagcaaaga ataaaattaa attaagaagg acaatggtgt 33180 cccaatcctt atacaaccaa cttccacaag aaagtcaagt cagagacaac aaaaaaacaa 33240 gcaaaggaaa ttttttaatt tgagttgtct tgtttgctgc ataatttatg cagtaaaaca 33300 ctacacataa cccttttagc agtagagcaa tggttgaccg tgtgcttagc ttcttttatt 33360 ttattttttt atcagcaaag aataaataaa ataaaatgag acacttcagg gatgtttcaa 33420 cccttataca aaaccccaaa aacaagtttc ctagcaccct accaacgaat tcgcggccgc 33480 ttaattaatc tgatcatgga tgctacgtca cggcagtaca ggactatcac tagacctagg 33540 agctgtgctg ggtaactcac atgagtcact ggtcacatca gcggccgcct cccagtatca 33600 ttatagtgaa agttttggct ctctcgccgg tggtttttta cctctattta aaggggtttt 33660 ccacctaaaa attctggtat cattctcact ttacttgtta ctttaatttc tcataatctt 33720 tggttgaaat tatcacgctt ccgcacacga tatccctaca aatttattat ttgttaaaca 33780 ttttcaaacc gcataaaatt ttatgaagtc ccgtctatct ttaatgtagt ctaacatttt 33840 catattgaaa tatataattt acttaatttt agcgttggta gaaagcataa tgatttattc 33900 ttattcttct tcatataaat gtttaatata caatataaac aaattcttta ccttaagaag 33960 gatttcccat tttatatttt aaaaatatat ttatcaaata tttttcaacc acgtaaatct 34020 cataataata agttgtttca aaagtaataa aatttaactc cataattttt ttattcgact 34080 gatcttaaag caacacccag tgacacaact agccattttt ttctttgaat aaaaaaatcc 34140 aattatcatt gtattttttt tatacaatga aaatttcacc aaacaatgat ttgtggtatt 34200 tctgaagcaa gtcatgttat gcaaaattct ataattccca tttgacacta cggaagtaac 34260 tgaagatctg cttttacatg cgagacacat cttctaaagt aattttaata atagttacta 34320 tattcaagat ttcatatatc aaatactcaa tattacttct aaaaaattaa ttagatataa 34380 ttaaaatatt acttttttaa ttttaagttt aattgttgaa tttgtgacta ttgatttatt 34440 attctactat gtttaaattg ttttatagat agtttaaagt aaatataagt aatgtagtag 34500 agtgttagag tgttacccta aaccataaac tataagattt atggtggact aattttcata 34560 tatttcttat tgcttttacc ttttcttggt atgtaagtcc gtaactggaa ttactgtggg 34620 ttgccatgac actctgtggt cttttggttc atgcatggat cttgcgcaag aaaaagacaa 34680 agaacaaaga aaaaagacaa aacagagaga caaaacgcaa tcacacaacc aactcaaatt 34740 agtcactggc tgatcaagat cgccgcgtcc atgtatgtct aaatgccatg caaagcaaca 34800 cgtgcttaac atgcacttta aatggctcac ccatctcaac ccacacacaa acacattgcc 34860 tttttcttca tcatcaccac aaccacctgt atatattcat tctcttccgc cacctcaatt 34920 tcttcacttc aacacacgtc aacctgcata tgcgtgtcat cccatgccca aatctccatg 34980 catgttccaa ccaccttctc tcttatataa tacctataaa tacctctaat atcactcact 35040 tctttcatca tccatccatc cagagtacta ctactctact actataatac cccaacccaa 35100 ctcatattca atactactct aggatccaca accaaaaatg cttcagcaca cttggcttcc 35160 gaagcctccc aatctgaccc tcttgtcaga tgaggttcat ctctggagga ttcctcttga 35220 ccagcctgag tcacaacttc aagaccttgc tgccaccctg agcagtgatg aattggcgag 35280 ggcaaacaga ttctactttc cagaacacag aaggcgtttc actgctggga gaggcatcct 35340 cagatccatc ttgggtggat acttgggagt ggaaccgggt caagtcaagt ttgattatga 35400 gtcccgtggg aaaccgatcc ttggtgacag atttgctgag agtggactcc tgttcaactt 35460 gtctcacagc cagaaccttg ccttgtgtgc tgtcaactac acgcgtcaaa taggcattga 35520 tcttgaatat ctgcgtccaa catctgactt ggagtctctt gcaaagaggt tctttctccc 35580 aagagaatat gaactcttga ggtcactccc tgatgagcag aaacagaaga ttttctttcg 35640 ttactggact tgcaaagagg cttatctcaa agcaacggga gatggaatag ccaaacttga 35700 agagatcgag atagcactca ccccaacaga acctgccaag ctccaaacag ctcctgcgtg 35760 gtctctgttg gagcttgtgc cagatgacaa ttgtgttgca gctgtggctg ttgcgggttt 35820 tggttggcag cccaagttct ggcattactg agtagttagc ttaatcactt aggtcaccag 35880 tatgaactaa aatgcatgta ggtgtaagag ctcatggaga gcatggaata ttgtatccga 35940 ccatgtaaca gtataataac tgagctccat ctcacttctt ctatgaataa acaaaggatg 36000 ttatgatata ttaacactct atctatgcac cttattgttc tatgataaat ttcctcttat 36060 tattataaat catctgaatc gtgacggctt atggaatgct tcaaatagta caaaaacaaa 36120 tgtgtactat aagactttct aaacaattct aactttagca ttgtgaacga gacataagtg 36180 ttaagaagac ataacaatta taatggaaga agtttgtctc catttatata ttatatatta 36240 cccacttatg tattatatta ggatgttaag gagacataac aattataaag agagaagttt 36300 gtatccattt atatattata tactacccat ttatatatta tacttatcca cttatttaat 36360 gtctttataa ggtttgatcc atgatatttc taatatttta gttgatatgt atatgaaaag 36420 gtactatttg aactctctta ctctgtataa aggttggatc atccttaaag tgggtctatt 36480 taattttatt gcttcttaca gataaaaaaa aaattatgag ttggtttgat aaaatattga 36540 aggatttaaa ataataataa ataataaata acatataata tatgtatata aatttattat 36600 aatataacat ttatctataa aaaagtaaat attgtcataa atctatacaa tcgtttagcc 36660 ttgctggaac gaatctcaat tatttaaacg agagtaaaca tatttgactt tttggttatt 36720 taacaaatta ttatttaaca ctatatgaaa tttttttttt ttatcagcaa agaataaaat 36780 taaattaaga aggacaatgg tgtcccaatc cttatacaac caacttccac aagaaagtca 36840 agtcagagac aacaaaaaaa caagcaaagg aaatttttta atttgagttg tcttgtttgc 36900 tgcataattt atgcagtaaa acactacaca taaccctttt agcagtagag caatggttga 36960 ccgtgtgctt agcttctttt attttatttt tttatcagca aagaataaat aaaataaaat 37020 gagacacttc agggatgttt caacccttat acaaaacccc aaaaacaagt ttcctagcac 37080 cctaccaacg aattcgcggc cgcttaatta actactgtca ctgaggccgt agacgagtac 37140 ggactgatct aactagtgct agcctcgagg tcgacaccca gctttcttgt acaaagtggt 37200 tgcggccgct taattaaatt taaattcaat taatgcaatc ttgattttca acaacgaagg 37260 taatggcgta aaagaaaaaa tgtatgttat tgtattgatc tttcatgatg ttgaagcgtg 37320 ccataatatg atgatgtata attaaaatat taactgtcgc attttattga aatggcactg 37380 ttatttcaac catatctttg attctgttac atgacacgac tgcaagaagt aaataataga 37440 cgccgttgtt aaagaattgc tatcatatgt gcctaactag agggaatttg agcgtcagac 37500 ctaatcaaat attacaaaat atctcactct gtcgccagca atggtgtaat cagcgcagac 37560 aaatggcgta aagatcgcgg aaaaacctcc ccgagtggca tgatagctgc ctctgtattg 37620 ctgatttagt cagccttatt tgacttaagg gtgccctcgt tagtgacaaa ttgctttcaa 37680 ggagacagcc atgccccaca ctttgttgaa aaacaaattg cctttgggga gacggtaaag 37740 ccagttgctc ttcaataagg aatgtcgagg aggcaatgta accgcctctg gtagtacact 37800 tctctaatcc aaaaatcaat ttgtattcaa gataccgcaa aaaacttatg gtttaaaccc 37860 tgcaggacta gtccagaagg taattatcca agatgtagca tcaagaatcc aatgtttacg 37920 ggaaaaacta tggaagtatt atgtaagctc agcaagaagc agatcaatat gcggcacata 37980 tgcaacctat gttcaaaaat gaagaatgta cagatacaag atcctatact gccagaatac 38040 gaagaagaat acgtagaaat tgaaaaagaa gaaccaggcg aagaaaagaa tcttgaagac 38100 gtaagcactg acgacaacaa tgaaaagaag aagataaggt cggtgattgt gaaagagaca 38160 tagaggacac atgtaaggtg gaaaatgtaa gggcggaaag taaccttatc acaaaggaat 38220 cttatccccc actacttatc cttttatatt tttccgtgtc atttttgccc ttgagttttc 38280 ctatataagg aaccaagttc ggcatttgtg aaaacaagaa aaaatttggt gtaagctatt 38340 ttctttgaag tactgaggat acaacttcag agaaatttgt aagtttgtag gtaccagatc 38400 tggatcccaa accatgtctc cggagaggag accagttgag attaggccag ctacagcagc 38460 tgatatggcc gcggtttgtg atatcgttaa ccattacatt gagacgtcta cagtgaactt 38520 taggacagag ccacaaacac cacaagagtg gattgatgat ctagagaggt tgcaagatag 38580 atacccttgg ttggttgctg aggttgaggg tgttgtggct ggtattgctt acgctgggcc 38640 ctggaaggct aggaacgctt acgattggac agttgagagt actgtttacg tgtcacatag 38700 gcatcaaagg ttgggcctag gatctacatt gtacacacat ttgcttaagt ctatggaggc 38760 gcaaggtttt aagtctgtgg ttgctgttat aggccttcca aacgatccat ctgttaggtt 38820 gcatgaggct ttgggataca cagcccgggg tacattgcgc gcagctggat acaagcatgg 38880 tggatggcat gatgttggtt tttggcaaag ggattttgag ttgccagctc ctccaaggcc 38940 agttaggcca gttacccaaa tctgagtagt tagcttaatc acctagagct cgatcggcgg 39000 caatagcttc ttagcgccat cccgggttga tcctatctgt gttgaaatag ttgcggtggg 39060 caaggctctc tttcagaaag acaggcggcc aaaggaaccc aaggtgaggt gggctatggc 39120 tctcagttcc ttgtggaagc gcttggtcta aggtgcagag gtgttagcgg gatgaagcaa 39180 aagtgtccga ttgtaacaag atatgttgat cctacgtaag gatattaaag tatgtattca 39240 tcactaatat aatcagtgta ttccaatatg tactacgatt tccaatgtct ttattgtcgc 39300 cgtatgtaat cggcgtcaca aaataatccc cggtgacttt cttttaatcc aggatgaaat 39360 aatatgttat tataattttt gcgatttggt ccgttatagg aattgaagtg tgcttgaggt 39420 cggtcgccac cactcccatt tcataatttt acatgtattt gaaaaataaa aatttatggt 39480 attcaattta aacacgtata cttgtaaaga atgatatctt gaaagaaata tagtttaaat 39540 atttattgat aaaataacaa gtcaggtatt atagtccaag caaaaacata aatttattga 39600 tgcaagttta aattcagaaa tatttcaata actgattata tcagctggta cattgccgta 39660 gatgaaagac tgagtgcgat attatggtgt aatacatagg aattcgttta aacgatctgc 39720 gtctaatttt cggtccaact tgcacaggaa agacgtcgac cgcggtagct cttgcccagc 39780 agactgggct tccagtcctt tcgctcgatc gggtccaatg ttgtcctcag ctgtgaaccg 39840 gaagcggacg accaacagtg gaagaactga aaggaacgag ccgtctatac cttgatgatc 39900 ggcctctggt gaagggtatc atcgcagcca agcaagctca tgaaaggctg atgggggagg 39960 tgtataatta tgaggcccac ggcgggctta ttctttaggg aggatctatc tcgttgctca 40020 agtgcatggc gcaaagcagt tattggagtg cggattttcg ttggcatatt attcgccacg 40080 agttagcaga cgaagagacc ttcatgaacg tggccaaggc cagagttaag cagatgttac 40140 gccctgctgc aggcctttct attatccaat agttggttga tctttggaaa gagcctcggc 40200 tgaggcccat actgaaagag atcgatggat atcgatatgc catgttgttt gctagccaga 40260 accagatcac atccgatatg ctattgcagc ttgacgcaga tatggaggat aagttgattc 40320 atgggatcgc tcaggagtag ctcatccatg cacgccgaca agaacagaaa ttccgtcgag 40380 ttaacgcagc cgcttacgac ggattcgaag gtcatccatt cggaatgtat tagtttgcac 40440 cagctccgcg tcacacctgt cttcatttga ataagatgtt agcaattgtt tttagctttg 40500 tcttgttgtg gcagggcggc aagtgcttca gacatcattc tgttttcaaa ttttatgctg 40560 gagaacagct tcttaattcc tttggaaata atagactgcg tcttaaaatt cagatgtctg 40620 gatatagata tgattgtaaa ataacctatt taagtgtcat ttagaacata agttttatga 40680 atgttcttcc attttcgtca tcgaacgaat aagagtaaat acaccttttt taacattaca 40740 aataagttct tatacgttgt ttatacaccg ggaatcattt ccattatttt cgcgcaaaag 40800 tcacggatat tcgtgaaagc gacataaact gcgaaatttg cggggagtgt cttgagtttg 40860 cctcgaggct agcgcatgca catagacaca cacatcatct cattgatgct tggtaataat 40920 tgtcattaga ttgtttttat gcatagatgc actcgaaatc agccaatttt agacaagtat 40980 caaacggatg tgacttcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 41040 cgtcaatttg atttacaatt gaatatatcc tgccccagcc agccaacagc tcgatttaca 41100 attgaatata tcctgccggc cggcccacgc gtgtcgagga attctgatct ggcccccatt 41160 tggacgtgaa tgtagacacg tcgaaataaa gatttccgaa ttagaataat ttgtttattg 41220 ctttcgccta taaatacgac ggatcgtaat ttgtcgtttt atcaaaatgt actttcattt 41280 tataataacg ctgcggacat ctacattttt gaattgaaaa aaaattggta attactcttt 41340 ctttttctcc atattgacca tcatactcat tgctgatcca tgtagatttc ccggacatga 41400 agccatttac aattgaatat atcctgccgc cgctgccgct ttgcacccgg tggagcttgc 41460 atgttggttt ctacgcagaa ctgagccggt taggcagata atttccattg agaactgagc 41520 catgtgcacc ttccccccaa cacggtgagc gacggggcaa cggagtgatc cacatgggac 41580 ttttaaacat catccgtcgg atggcgttgc gagagaagca gtcgatccgt gagatcagcc 41640 gacgcaccgg gcaggcgcgc aacacgatcg caaagtattt gaacgcaggt acaatcgagc 41700 cgacgttcac gcggaacgac caagcaagct tggctgccat ttttggggtg aggccgttcg 41760 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 41820 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 41880 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 41940 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 42000 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 42060 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 42120 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 42180 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 42240 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 42300 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 42360 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 42420 agcccggtga gcgtcggaaa gggtcgacgg atcttttccg ctgcataacc ctgcttcggg 42480 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc 42540 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt 42600 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg 42660 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag 42720 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg 42780 tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc 42840 ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat 42900 gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg cggcctgctg 42960 aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac gatcctcgcc 43020 ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat gggcgtggtc 43080 cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg tgcgcgtgat 43140 tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc tggtattcgt 43200 gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga 43260 cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga 43320 ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg 43380 gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga 43440 tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt 43500 cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc 43560 ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg aacaggaggc 43620 ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg 43680 aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct 43740 gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg 43800 gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg 43860 caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga 43920 cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca 43980 gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta 44040 cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga 44100 atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct 44160 ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg 44220 ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa 44280 attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt 44340 cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc 44400 ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg 44460 aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta 44520 gggccttgtg gggtcagttc cggctggggg ttcagcagcc agcgctttac tggcatttca 44580 ggaacaagcg ggcactgctc gacgcacttg cttcgctcag tatcgctcgg gacgcacggc 44640 gcgctctacg aactgccgat aaacagagga ttaaaattga caattgtgat taaggctcag 44700 attcgacggc ttggagcggc cgacgtgcag gatttccgcg agatccgatt gtcggccctg 44760 aagaaagctc cagagatgtt cgggtccgtt tacgagcacg aggagaaaaa gcccatggag 44820 gcgttcgctg aacggttgcg agatgccgtg gcattcggcg cctacatcga cggcgagatc 44880 attgggctgt cggtcttcaa acaggaggac ggccccaagg acgctcacaa ggcgcatctg 44940 tccggcgttt tcgtggagcc cgaacagcga ggccgagggg tcgccggtat gctgctgcgg 45000 gcgttgccgg cgggtttatt gctcgtgatg atcgtccgac agattccaac gggaatctgg 45060 tggatgcgca tcttcatcct cggcgcactt aatatttcgc tattctggag cttgttgttt 45120 atttcggtct accgcctgcc gggcggggtc gcggcgacgg taggcgctgt gcagccgctg 45180 atggtcgtgt tcatctctgc cgctctgcta ggtagcccga tacgattgat ggcggtcctg 45240 ggggctattt gcggaactgc gggcgtggcg ctgttggtgt tgacaccaaa cgcagcgcta 45300 gatcctgtcg gcgtcgcagc gggcctggcg ggggcggttt ccatggcgtt cggaaccgtg 45360 ctgacccgca agtggcaacc tcccgtgcct ctgctcacct ttaccgcctg gcaactggcg 45420 gccggaggac ttctgctcgt tccagtagct ttagtgtttg atccgccaat cccgatgcct 45480 acaggaacca atgttctcgg cctggcgtgg ctcggcctga tcggagcggg tttaacctac 45540 ttcctttggt tccgggggat ctcgcgactc gaacctacag ttgtttcctt actgggcttt 45600 ctcagccccc gagcgcttag tgggaatttg taccccttat cgaaccggga gcacaggatg 45660 acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt caggagttaa 45720 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg 45780 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg 45840 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg 45900 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag 45960 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt 46020 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg 46080 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa 46140 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg 46200 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact 46260 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa 46320 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc 46380 agtatcagcc cgtcatactt gaagctaggc aggcttatct tggacaagaa gatcgcttgg 46440 cctcgcgcgc agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg 46500 tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 46560 actcaagcgt tagagagctg gggaagacta tgcgcgatct gttgaaggtg gttctaagcc 46620 tcgtcttgcg atggcatttc gatccattcc cattccgcgc tcaagatggc ttcccctcgg 46680 cagttcatca gggctaaatc aatctagccg acttgtccgg tgaaatgggc tgcactccaa 46740 cagaaacaat caaacaaaca tacacagcga cttattcaca cgagctcaaa ttacaacggt 46800 atatatcctg ccagtcagca tcatcacacc aaaagttagg cccgaatagt ttgaaattag 46860 aaagctcgca attgaggtct acaggccaaa ttcgctctta gccgtacaat attactcacc 46920 ggatcctaac cggtttaatt aaggcgcgcc atgcccgggc aagcggccgc acaagttt 46978 <210> 52 <211> 41726 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9337 <400> 52 gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat ctcagcatgt 60 gcgtagcacg cggccgcctc ccagtatcat tatagtgaaa gttttggctc tctcgccggt 120 ggttttttac ctctatttaa aggggttttc cacctaaaaa ttctggtatc attctcactt 180 tacttgttac tttaatttct cataatcttt ggttgaaatt atcacgcttc cgcacacgat 240 atccctacaa atttattatt tgttaaacat tttcaaaccg cataaaattt tatgaagtcc 300 cgtctatctt taatgtagtc taacattttc atattgaaat atataattta cttaatttta 360 gcgttggtag aaagcataat gatttattct tattcttctt catataaatg tttaatatac 420 aatataaaca aattctttac cttaagaagg atttcccatt ttatatttta aaaatatatt 480 tatcaaatat ttttcaacca cgtaaatctc ataataataa gttgtttcaa aagtaataaa 540 atttaactcc ataatttttt tattcgactg atcttaaagc aacacccagt gacacaacta 600 gccatttttt tctttgaata aaaaaatcca attatcattg tatttttttt atacaatgaa 660 aatttcacca aacaatgatt tgtggtattt ctgaagcaag tcatgttatg caaaattcta 720 taattcccat ttgacactac ggaagtaact gaagatctgc ttttacatgc gagacacatc 780 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 840 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 900 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 960 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 1020 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 1080 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 1140 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 1200 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 1260 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 1320 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 1380 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 1440 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 1500 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 1560 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 1620 ccaaaaatgg cagcgaggct gcaagagcag aaaggaggtg agatggacac gaggattgcg 1680 atcataggca tgagcgccat acttccttgt ggaaccacgg tgagagagtc atgggagacc 1740 ataagggctg gcattgattg cctgtctgat cttcctgagg atcgtgttga tgtgactgcg 1800 tactttgacc cagtcaagac cacaaaggac aaaatctact gcaagagagg aggtttcatt 1860 cctgagtatg actttgatgc gcgtgagttt ggtctcaaca tgttccagat ggaagattct 1920 gatgcgaatc agaccatctc acttctcaag gtcaaagaag cacttcaaga tgctgggatt 1980 gatgcacttg gaaaggagaa gaaaaacata ggatgcgtgc ttggcattgg aggtggacag 2040 aagagttcac atgagttcta cagcagactc aactatgtgg ttgtggagaa agtcttgaga 2100 aagatgggga tgccagagga agatgtcaaa gtggctgtgg agaagtacaa agcgaacttc 2160 ccagaatgga gacttgactc tttccctgga ttccttggga atgtcactgc tggcagatgc 2220 accaacacat tcaacttgga tggcatgaac tgcgttgtgg atgctgcatg tgcaagctcc 2280 ttgatagcag tcaaggttgc gattgatgaa ctgctctatg gtgactgcga catgatggtc 2340 actggtgcaa cctgcacaga caactccatt gggatgtaca tggctttctc aaagacacct 2400 gtgttcagca cggacccaag tgtgagagcg tatgatgaga aaacgaaagg gatgctcatt 2460 ggtgagggat cagccatgtt ggtgctcaaa cgttatgcag atgcagtgag ggatggtgat 2520 gagattcatg ctgtgattcg tggttgtgca agctcaagtg atggcaaagc agctggcatc 2580 tacactccca caatcagtgg tcaagaggaa gccttgagac gtgcatacaa tcgtgcctgc 2640 gttgaccctg ccactgtcac tttggttgag ggtcatggga ctggcactcc agttggagat 2700 aggattgagt tgactgcgtt gaggaacttg tttgacaaag cgtatggtga agggaacaca 2760 gagaaagttg cagttggaag tatcaagtcc agtattggtc atttgaaggc tgttgctggt 2820 cttgctggaa tgatcaaggt catcatggct ctcaaacaca agactttgcc tggaacgatc 2880 aatgtggaca atccacccaa tctctatgac aacactccca tcaatgagtc atccctctac 2940 atcaacacga tgaacagacc ctggttccct ccacctggtg ttccgagacg tgctggaata 3000 tccagctttg gctttggagg tgccaactac catgcagtgt tggaagaggc tgaaccagag 3060 cacacgacag cgtacagact caacaaacgt ccacaaccgg tgttgatgat ggctgcaact 3120 cctgctgcgt tgcagagcct ttgtgaagct cagctgaaag agtttgaagc tgcgatcaag 3180 gagaatgaga cagtcaagaa tactgcctac atcaagtgtg tcaagtttgg agaacagttc 3240 aagttccctg ggagcatacc tgcgaccaat gcgagactgg gattcttggt caaagatgcg 3300 gaggatgcgt gctccacgct tcgtgctata tgtgctcagt ttgccaaaga tgtgaccaaa 3360 gaagcgtgga ggcttccgag agaaggagtt tctttccgtg ccaaaggcat tgccaccaat 3420 ggtgctgttg ctgcactctt ctctggacaa ggtgctcagt acacacacat gttcagtgaa 3480 gttgcgatga actggcctca gttcagacag tccattgctg caatggacgc tgcacagtcc 3540 aaggttgctg gaagtgacaa agactttgaa agagtctctc aagtcctgta cccaaggaaa 3600 ccgtacgaac gtgagccaga acaagatcac aagaaaatct cactcacagc atacagccag 3660 ccgtcaaccc tggcatgtgc tcttggtgcc tttgagattt tcaaggaagc tggattcact 3720 ccagactttg cagctggaca cagcttgggt gagtttgctg cgctctatgc agctggttgt 3780 gttgacagag atgagttgtt tgaacttgtt tgcagaaggg caaggatcat gggaggcaaa 3840 gatgcacctg ccacacccaa aggttgcatg gcagctgtca taggacccaa cgcagagaac 3900 atcaaggttc aagctgccaa cgtctggctt ggaaactcaa actctccgtc tcagactgtc 3960 attactgggt ctgttgaagg gattcaagct gaaagtgcca gattgcagaa ggaagggttc 4020 agagttgtgc ctcttgcttg tgagtctgcc ttccattctc cacagatgga aaatgccagc 4080 tcagctttca aagatgtcat atccaaggtt tccttccgta cacccaaagc agaaacaaag 4140 ctcttcagca atgtttctgg agagacctat ccgacggatg ccagagagat gctcactcaa 4200 cacatgacct catctgtgaa gttcctgacc caagtgagga acatgcacca agctggtgcg 4260 agaatctttg ttgagtttgg accgaaacaa gttctcagca aacttgtttc tgaaaccctc 4320 aaagatgacc cgtctgttgt cacagtgtct gtgaacccag cttctggcac ggactctgac 4380 atacagttga gggatgctgc agttcagctg gttgtggctg gtgtcaatct tcaagggttt 4440 gacaagtggg atgctcctga tgccacgagg atgcaagcca tcaagaaaaa gagaaccaca 4500 ctcagacttt cagctgccac ctatgtcagt gacaagacca agaaagtgag ggatgctgcc 4560 atgaacgacg gaagatgtgt cacatacctc aagggtgctg caccactcat caaggctcct 4620 gaacctgtgg ttgacgaagc tgccaagagg gaggctgaaa ggcttcagaa ggagcttcaa 4680 gacgctcaga ggcagcttga cgacgccaaa cgtgcagctg cagaagccaa ctccaaactt 4740 gctgctgcca aggaggaagc caagactgct gcagcttcag ccaagcctgc agttgacact 4800 gctgttgtgg agaaacatcg tgccatcctc aagtccatgc tggctgagct ggatggttat 4860 ggaagtgttg atgcttcttc ccttcagcaa cagcaacagc aacagactgc tccggcgcct 4920 gttaaagccg ctgcccctgc cgctcccgtt gcaagtgctc ctgctcccgc cgtgtccaac 4980 gagttgcttg agaaagctga gacagttgtg atggaggttc tcgctgccaa gactggatac 5040 gaaacagaca tgattgaggc ggacatggag cttgagactg agttgggaat tgacagcatc 5100 aagagggttg aaattctttc tgaagtccaa gctatgctta acgttgaggc gaaagatgtt 5160 gatgctctct ctaggacgag aacagtgggt gaagttgtca acgctatgaa ggcagaaatc 5220 gctggttctt ccgctccggc gccggcagct gccgcacctg caccggctaa agcggctcca 5280 gctgcagctg caccagctgt ttctaatgag ctgctcgaaa aggcagaaac tgttgtgatg 5340 gaagtccttg ctgccaaaac tggctacgaa actgacatga tagaatcaga catggagctt 5400 gagacggagc ttggaattga ttccatcaag cgtgtggaaa tcctcagtga agtccaagcc 5460 atgctcaatg tggaagctaa ggatgtggat gctctctcaa ggaccagaac tgtcggtgag 5520 gttgtcaatg ccatgaaggc tgagatagct ggaggctccg ctcctgcgcc ggccgctgcc 5580 gctcctggtc ccgctgccgc ggctccagct ccagctgcag ccgctccggc cgtctcaaac 5640 gaactccttg aaaaagctga gaccgtcgtg atggaggtct tggcagctaa aaccggctat 5700 gagactgaca tgattgagtc tgacatggaa ttggagacag aacttgggat agacagcatc 5760 aaacgtgttg aaatcctcag tgaggtccaa gctatgctga acgtggaagc gaaggatgtt 5820 gatgctctgt ccagaacgag gaccgttggt gaggttgtgg atgccatgaa ggctgaaatt 5880 gctggagggt ctgcgcccgc gccagctgca gccgctcctg cacccgcagc cgcggcacct 5940 gctccagctg cacctgcacc tgccgtttct tcagaactct tggagaaggc tgagacggtt 6000 gtgatggagg ttttggctgc aaagactggc tatgagaccg acatgattga atctgatatg 6060 gaattggaga cagagcttgg gatcgattcc atcaaaagag tggagatcct gagtgaagtc 6120 caagccatgc tcaatgttga agctaaggat gttgatgcac tttcaagaac aaggacagtg 6180 ggtgaggttg tggatgccat gaaggcggag attgctggag ggagcgcgcc tgcgccagct 6240 gccgcggcac ctgcaccggc agccgcagct ccagctccag ctgcaccagc accggccgcg 6300 cccgcgccag ctgtttcatc cgaactcctg gaaaaggccg agacggttgt gatggaggtg 6360 ctcgcagcta agactggtta cgagaccgac atgattgaaa gtgacatgga gcttgaaacc 6420 gagttgggaa tagatagcat caaacgtgtt gaaatcttgt ctgaggtcca agctatgttg 6480 aacgtggagg caaaagatgt cgatgcgctt tcaagaacca gaacagtcgg tgaggtcgtg 6540 gacgccatga aggctgaaat tgccggaagc tctgctagtg cgccagctgc agccgcacct 6600 gcacctgctg ccgcggcacc tgctccagct gcagccgctc ctgccgtcag caatgagttg 6660 ctggagaaag ccgaaacagt tgtgatggaa gtccttgctg caaaaactgg ttacgaaacg 6720 gacatgattg agtcagacat ggaattggag accgaacttg gaatagattc catcaagaga 6780 gtggagatcc tctctgaggt gcaagccatg ctcaacgttg aggctaaaga tgttgatgca 6840 ctcagtagga caagaactgt gggggaagtt gtcgatgcga tgaaggctga gattgctgga 6900 ggctctgctc ctgcgccggc agctgccgca cctgcacccg cagccgcggc accagctgtc 6960 agcaacgaat tgcttgagaa ggctgaaaca gttgtcatgg aagtgctggc tgcaaaaacc 7020 ggctatgaga ctgacatgat tgagtctgac atggaacttg agacggaatt gggcattgac 7080 agcatcaagc gtgttgagat cctcagtgaa gtccaagcaa tgctcaatgt ggaggctaag 7140 gatgtggatg ccctctcaag gaccagaaca gttggtgagg tcgttgatgc gatgaaggcg 7200 gagatcgctg gaagttccgc gcctgcgcca gctgcagccg ctcctgcacc cgcagccgcg 7260 gcaccggctc cagctgcagc cgcaccggcc gtctcctcag aactcctgga aaaggctgag 7320 acagttgtga tggaagtcct tgctgcgaaa actggctatg agaccgacat gattgaatct 7380 gacatggaac tggagaccga gcttggaatt gactccatca aacgtgttga aatcctctct 7440 gaggttcaag cgatgttgaa tgtggaggcc aaagatgttg atgctctttc cagaacaagg 7500 acggtgggag aggtggttga tgccatgaag gctgagatag ctgggggttc tgctcctgcg 7560 ccagctgcag ccgctcctgc tcccgctgcc gcggcaccag ctgtcagcaa cgaactcctt 7620 gaaaaagctg aaactgttgt gatggaggtt ttggctgcaa agactggata cgagactgac 7680 atgatagagt cagacatgga gttggaaaca gagcttggga ttgatagcat caagcgtgtg 7740 gaaatccttt ctgaggttca agccatgctg aacgttgagg ccaaagatgt cgatgctttg 7800 tcaaggacca gaacggttgg agaagtggtc gatgccatga aggctgagat agctggtgga 7860 tcagcccctg ctccggctgc agccgctcct gcaagtgccg gtgctgcacc agctgtgaag 7920 attgattcag ttcatggtgc tgactgtgac gatttgtcac tgatgcacgc aaaggtggtt 7980 gacatcagaa ggccagatga actgattctg gaaagaccgg agaaccgtcc agttcttgtc 8040 gttgacgatg gctcagagtt gacacttgct cttgtgaggg tgttgggagc ctgtgcagtt 8100 gtcttgacct ttgagggatt gcagttggct cagagagctg gtgcagctgc cattcgtcat 8160 gttctggcta aggacctttc agcagaatca gctgagaagg ccatcaaaga ggctgagcaa 8220 agatttggag cgttgggagg cttcataagc cagcaagcag aaaggtttga accagctgag 8280 atactgggtt tcacactcat gtgtgcgaag tttgccaaag cctctctctg cactgctgtg 8340 gctggaggtc gtcctgcctt catcggtgtg gctaggcttg acggcagact tggattcacc 8400 tctcaaggca cttctgacgc actcaagaga gcacagcgtg gagcgatctt tggactctgc 8460 aagacaatag ggttggagtg gtcagaatct gatgtgttca gccgtggtgt ggacattgct 8520 caagggatgc acccagaaga tgctgcagtt gccatagtta gggagatggc gtgtgctgac 8580 atcagaatca gagaggttgg gattggagca aaccagcaaa gatgcacaat cagagctgcc 8640 aagttggaga ctgggaaccc tcagagacag atagcgaaag acgatgtgct tctggtttct 8700 ggaggtgctc gtgggatcac acctctttgc atccgtgaga tcacgcgtca gattgctgga 8760 ggcaagtaca ttctgttggg tcgtagcaaa gtttctgcgt ctgaaccagc atggtgtgct 8820 ggcatcactg atgagaaggc tgtgcagaaa gctgccaccc aagagttgaa gagggccttc 8880 agtgctggtg aaggacccaa acccactcca agggctgtca cgaaacttgt gggatctgtt 8940 cttggtgctc gtgaagtgag aagctccatt gctgccattg aagctctggg tggcaaagcg 9000 atctacagct cttgtgatgt gaactctgct gcagatgttg ccaaagcagt tagggatgca 9060 gagtcccagc ttggtgccag agttagtgga atcgttcacg cttctggtgt gctccgtgat 9120 cgtctcatag agaagaaact ccctgacgag tttgatgctg tctttgggac caaagtcact 9180 gggttggaga atcttctggc tgcagttgat cgtgccaacc tcaaacacat ggttctcttc 9240 agctcccttg ctggtttcca cggcaatgtt ggtcagtctg actatgcgat ggcaaacgaa 9300 gctctcaaca agatgggttt ggaacttgcg aaggatgtca gtgtcaagtc catctgcttt 9360 ggtccctggg atggtgggat ggtgacacct caactcaaga aacagtttca agaaatggga 9420 gttcagatca ttcctcgtga aggaggtgct gatacagttg cgaggattgt gttgggatca 9480 agtcctgctg agatccttgt tggaaactgg aggacaccat ccaagaaagt tggaagtgac 9540 acgatcaccc tgcatagaaa gatcagtgcc aagtccaatc ccttcttgga ggatcatgtc 9600 atacaaggca gaagggtgtt gccaatgacc cttgctattg ggagccttgc tgagacctgc 9660 cttggactct tccctggcta ctctctctgg gccatagatg acgcacagct cttcaaaggt 9720 gtcactgttg atggagacgt caactgtgaa gtgaccctca caccgtccac tgctccgagt 9780 gggagggtga atgtccaagc gacgctcaag acgttctcaa gtggaaaact tgttccagcc 9840 tacagagctg tcatagtgtt gtccaaccaa ggtgctccac ctgccaatgc aaccatgcag 9900 cctccgagcc ttgatgcaga tcctgctttg caagggtcag tctatgatgg caagactctc 9960 tttcatggtc ctgcgttcag aggcattgat gacgttctca gctgcaccaa gagccagctt 10020 gttgccaagt gttctgctgt tcctggatca gatgcagcca gaggagagtt tgcaactgac 10080 actgatgcac atgatccctt tgtcaatgat cttgcctttc aagcaatgtt ggtctgggtg 10140 aggagaacgc tgggacaagc tgcactcccg aactccattc agaggatagt ccaacacaga 10200 ccggtcccac aagacaaacc cttctacatc accctcagat ccaatcagag tggtggacat 10260 agccagcaca aacatgcact tcagttccac aacgaacaag gtgatctctt cattgatgtt 10320 caagcctctg tcattgcgac agactcactt gcgttctgag tagttagctt aatcacttag 10380 gtcaccagta tgaactaaaa tgcatgtagg tgtaagagct catggagagc atggaatatt 10440 gtatccgacc atgtaacagt ataataactg agctccatct cacttcttct atgaataaac 10500 aaaggatgtt atgatatatt aacactctat ctatgcacct tattgttcta tgataaattt 10560 cctcttatta ttataaatca tctgaatcgt gacggcttat ggaatgcttc aaatagtaca 10620 aaaacaaatg tgtactataa gactttctaa acaattctaa ctttagcatt gtgaacgaga 10680 cataagtgtt aagaagacat aacaattata atggaagaag tttgtctcca tttatatatt 10740 atatattacc cacttatgta ttatattagg atgttaagga gacataacaa ttataaagag 10800 agaagtttgt atccatttat atattatata ctacccattt atatattata cttatccact 10860 tatttaatgt ctttataagg tttgatccat gatatttcta atattttagt tgatatgtat 10920 atgaaaaggt actatttgaa ctctcttact ctgtataaag gttggatcat ccttaaagtg 10980 ggtctattta attttattgc ttcttacaga taaaaaaaaa attatgagtt ggtttgataa 11040 aatattgaag gatttaaaat aataataaat aataaataac atataatata tgtatataaa 11100 tttattataa tataacattt atctataaaa aagtaaatat tgtcataaat ctatacaatc 11160 gtttagcctt gctggaacga atctcaatta tttaaacgag agtaaacata tttgactttt 11220 tggttattta acaaattatt atttaacact atatgaaatt tttttttttt atcagcaaag 11280 aataaaatta aattaagaag gacaatggtg tcccaatcct tatacaacca acttccacaa 11340 gaaagtcaag tcagagacaa caaaaaaaca agcaaaggaa attttttaat ttgagttgtc 11400 ttgtttgctg cataatttat gcagtaaaac actacacata acccttttag cagtagagca 11460 atggttgacc gtgtgcttag cttcttttat tttatttttt tatcagcaaa gaataaataa 11520 aataaaatga gacacttcag ggatgtttca acccttatac aaaaccccaa aaacaagttt 11580 cctagcaccc taccaacgaa ttcgcggccg cttaattaag atgagtgata ctcaggactc 11640 aggactcact ctgctgatca ctagtgctag cctcgaggtc gacacaactt tgtatacaaa 11700 agttgtctag acctaggctc agcaactcat ccagtcaagt tcgagtcagt gagttgggcg 11760 gccgcctccc agtatcatta tagtgaaagt tttggctctc tcgccggtgg ttttttacct 11820 ctatttaaag gggttttcca cctaaaaatt ctggtatcat tctcacttta cttgttactt 11880 taatttctca taatctttgg ttgaaattat cacgcttccg cacacgatat ccctacaaat 11940 ttattatttg ttaaacattt tcaaaccgca taaaatttta tgaagtcccg tctatcttta 12000 atgtagtcta acattttcat attgaaatat ataatttact taattttagc gttggtagaa 12060 agcataatga tttattctta ttcttcttca tataaatgtt taatatacaa tataaacaaa 12120 ttctttacct taagaaggat ttcccatttt atattttaaa aatatattta tcaaatattt 12180 ttcaaccacg taaatctcat aataataagt tgtttcaaaa gtaataaaat ttaactccat 12240 aattttttta ttcgactgat cttaaagcaa cacccagtga cacaactagc catttttttc 12300 tttgaataaa aaaatccaat tatcattgta ttttttttat acaatgaaaa tttcaccaaa 12360 caatgatttg tggtatttct gaagcaagtc atgttatgca aaattctata attcccattt 12420 gacactacgg aagtaactga agatctgctt ttacatgcga gacacatctt ctaaagtaat 12480 tttaataata gttactatat tcaagatttc atatatcaaa tactcaatat tacttctaaa 12540 aaattaatta gatataatta aaatattact tttttaattt taagtttaat tgttgaattt 12600 gtgactattg atttattatt ctactatgtt taaattgttt tatagatagt ttaaagtaaa 12660 tataagtaat gtagtagagt gttagagtgt taccctaaac cataaactat aagatttatg 12720 gtggactaat tttcatatat ttcttattgc ttttaccttt tcttggtatg taagtccgta 12780 actggaatta ctgtgggttg ccatgacact ctgtggtctt ttggttcatg catggatctt 12840 gcgcaagaaa aagacaaaga acaaagaaaa aagacaaaac agagagacaa aacgcaatca 12900 cacaaccaac tcaaattagt cactggctga tcaagatcgc cgcgtccatg tatgtctaaa 12960 tgccatgcaa agcaacacgt gcttaacatg cactttaaat ggctcaccca tctcaaccca 13020 cacacaaaca cattgccttt ttcttcatca tcaccacaac cacctgtata tattcattct 13080 cttccgccac ctcaatttct tcacttcaac acacgtcaac ctgcatatgc gtgtcatccc 13140 atgcccaaat ctccatgcat gttccaacca ccttctctct tatataatac ctataaatac 13200 ctctaatatc actcacttct ttcatcatcc atccatccag agtactacta ctctactact 13260 ataatacccc aacccaactc atattcaata ctactctagg atccacaacc aaaaatggct 13320 gcaaggaacg tgagtgcggc tcatgagatg catgacgaga agaggatagc tgttgtgggc 13380 atggctgttc agtatgctgg atgcaaaacg aaagacgagt tctgggaagt tctcatgaat 13440 ggaaaagttg agagcaaagt gatcagcgac aaacgtcttg gttcaaatta ccgtgctgaa 13500 cactacaaag ctgaaaggag caagtatgct gacaccttct gcaatgagac atatggaact 13560 cttgatgaga acgaaataga caatgagcat gaactccttt tgaaccttgc caaacaagcc 13620 ttggctgaaa catctgtcaa agattcaaca agatgtggca tagtttctgg atgcctcagc 13680 ttcccgatgg acaacctcca aggtgaactc ttgaatgtgt accagaacca tgttgagaag 13740 aaacttggtg ccagagtttt caaagatgcc tctcactggt cagaaagaga acagtccaac 13800 aaaccagagg ctggtgacag aagaatcttc atggaccctg cttcctttgt tgctgaggaa 13860 ctcaatcttg gcgctctcca ctactctgtt gatgctgcct gtgccactgc gctctatgtg 13920 ctcagactgg cgcaagatca cttggtctct ggtgcagctg atgtgatgct ttgtggtgca 13980 acatgcctgc cggagccgtt tttcatactc tctgggttct caaccttcca agccatgcct 14040 gttggaacgg gtcagaatgt gtccatgccg ctgcacaaag attctcaagg attgacccct 14100 ggtgaaggag gttccatcat ggtcctcaaa cgtttggatg acgcaatcag agatggagat 14160 cacatctatg gaacactttt gggagccaat gtcagcaaca gtggcactgg tctgccactt 14220 aaaccccttt tgccgtcaga gaaaaagtgc ctcatggaca cctacacacg tatcaatgtg 14280 catccccaca agattcagta tgttgaatgc catgcaactg ggactccaca aggtgatcgt 14340 gtggaaattg atgctgtgaa ggcttgcttt gaaggcaaag tcccacgttt tggaaccaca 14400 aaagggaact ttggacacac attggtggca gctgggtttg ctggcatgtg caaagtgctt 14460 ctctccatga agcacgggat cattccaccc actccgggaa tagatgacga aacgaagatg 14520 gaccctctgg ttgtctctgg tgaggccatt ccctggccag aaacgaatgg agaaccgaag 14580 cgtgctggtc tctcagcgtt tggatttggt gggaccaatg ctcatgctgt ctttgaggaa 14640 catgacccaa gcaatgctgc atgcactggc catgactcca tttctgctct ctctgcacgt 14700 tgtggtggtg agtcaaacat gagaatagca atcactggga tggatgccac ttttggtgct 14760 ttgaaaggat tggatgcttt tgaaagggcc atctacactg gtgctcatgg tgctatacca 14820 ctcccagaaa agagatggag attcctgggg aaagacaagg acttccttga tctttgtgga 14880 gtcaaagcaa caccgcatgg ctgttacatt gaagatgttg aagttgattt ccagaggctg 14940 aggactccaa tgactcctga agatatgctc ttgcctcaac agcttctggc tgtcaccaca 15000 attgataggg ccatattgga ctctggcatg aagaaaggtg gaaatgtggc tgtctttgtg 15060 gggttgggga ccgatcttga actctaccgt caccgtgcaa gggttgctct caaggagaga 15120 gtgaggcctg aagccagcaa gaaactcaat gacatgatgc agtacatcaa tgattgtggg 15180 acaagcactt cttacaccag ctacataggc aacttggttg caacaagggt cagttcacaa 15240 tggggcttca cgggaccttc cttcacaatc acagaaggca acaattctgt gtacagatgt 15300 gctgaacttg gcaaatacct cttggaaacg ggagaggttg atggggttgt ggttgcgggt 15360 gttgatctct gtgggagtgc tgagaacctt tacgtcaagt caaggcgttt caaggtgtct 15420 acatctgaca ccccgagagc cagctttgat gcagctgctg atggttactt tgttggagag 15480 ggctgtggtg catttgtgct caagcgtgag acaagctgca ccaaggatga cagaatctat 15540 gcttgcatgg atgccatagt tcctggaaat gttccatctg catgtttgag agaggcattg 15600 gatcaagcca gagtcaagcc tggagacatc gagatgttgg agctttctgc tgattcagcg 15660 aggcatttga aggacccgtc tgttctccca aaggaactca ctgctgagga agagattggc 15720 ggacttcaga cgattcttcg tgacgatgac aagctcccaa ggaatgttgc aactggaagt 15780 gtcaaggcca ctgttggtga cactggttat gcttctggtg ctgccagttt gattaaggct 15840 gcgctttgca tctacaaccg ttacctgccc agcaatggag atgactggga tgaacctgca 15900 cctgaagcac cctgggacag cacactcttt gcgtgccaga cctcaagagc ttggttgaag 15960 aaccctggtg agagacgtta tgcggctgtt tccggagtca gtgaaaccag aagttgctac 16020 agtgtgctgc tttctgaagc agagggacac tacgagagag agaacagaat cagtttggat 16080 gaggaagcac ccaagctcat tgtgttgaga gcagactccc atgaagagat tcttggaaga 16140 ttggacaaga taagggaaag gttcttgcag cccacgggtg ctgcacccag agagtctgaa 16200 ttgaaggcgc aagcccgtag gatctttctt gaactccttg gtgagaccct tgcccaagat 16260 gcagcttcat ctggaagtca gaagcccctt gctctcagct tggtctccac tccatccaag 16320 ctccagagag aggtggaact tgcggcaaaa ggcattccga gatgtctcaa gatgagacgt 16380 gattggagtt cccctgctgg aagcagatat gcgcctgagc ctttggccag tgacagagtt 16440 gccttcatgt atggagaagg aaggagccct tactatggca tcacacaaga cattcatagg 16500 atttggcctg aacttcatga ggtcatcaat gagaaaacaa accgtctctg ggctgaaggt 16560 gacagatggg tcatgccaag ggccagcttc aagagtgagc ttgagtcaca acagcaagag 16620 tttgacagaa acatgattga aatgttccgt cttggaatcc tcacctcaat tgccttcaca 16680 aacctggcga gagatgttct caacatcacg cccaaagcag cctttggact cagtttggga 16740 gagatttcca tgatctttgc tttctccaag aaaaacggtt tgataagcga tcagttgaca 16800 aaggatttga gagagtcaga tgtctggaac aaagctctgg ctgttgagtt caatgcgctc 16860 agagaagcct ggggcattcc acagtctgtc ccaaaagatg agttctggca aggttacata 16920 gtgaggggaa cgaaacaaga catagaagca gccatagcac cagactcaaa gtatgtgagg 16980 ttgaccatca tcaatgatgc caacactgct ctcatcagtg gaaagcctga tgcatgcaaa 17040 gctgcgattg ctcgtcttgg tggcaacatc cctgcgcttc cagtcactca aggcatgtgt 17100 gggcattgcc cagaggtggg accttacacc aaggacatcg caaagatcca cgccaacctt 17160 gagttccctg tggttgatgg tcttgatctc tggactacca taaaccagaa gaggcttgtg 17220 ccaagagcca cgggtgccaa agatgaatgg gctccttcat cctttgggga gtatgctgga 17280 cagctctatg agaaacaagc caacttccct cagatagttg aaaccatcta caagcagaac 17340 tatgatgtgt ttgttgaagt tgggccaaac aatcacagaa gcacagcagt gagaaccacg 17400 cttggaccac agagaaacca ccttgctggt gccatagaca agcagaacga ggatgcttgg 17460 acgacaatag tcaagctcgt ggcttcattg aaggctcacc ttgttcctgg agtgaccatc 17520 agtccgctct accactccaa gctcgttgca gaggctgagg cttgttatgc tgcgctctgt 17580 aaaggtgaga aacccaagaa aaacaagttt gtgagaaaga ttcagttgaa tggcagattc 17640 aacagcaagg cagaccccat ctcctcagca gaccttgcat ccttcccacc tgcggaccct 17700 gcgattgaag ctgccatctc ttcaaggatc atgaagcctg tggctccgaa attctatgcg 17760 cgtctcaaca ttgatgagca agatgagacc agagatccga tcttgaacaa ggacaatgcg 17820 ccttccagct ccagctcttc cagcagttcc tcttccagca gttcatctcc gtctcctgct 17880 ccttcagctc cagttcagaa gaaagctgcc ccagctgcgg agacaaaagc tgttgccagt 17940 gctgatgcac ttcgtagtgc cctgcttgat cttgactcca tgcttgcgct cagctctgca 18000 agtgcttctg gcaaccttgt tgagactgcg ccatctgatg ccagtgtcat tgtccctccg 18060 tgcaacattg ctgatcttgg cagcagagct ttcatgaaaa cttatggtgt ttctgcgcct 18120 ctgtacaccg gtgcaatggc caagggaatt gcctctgcgg acttggtgat cgcagctgga 18180 aggcaaggaa tccttgcgtc atttggtgct ggaggccttc caatgcaagt tgtgcgtgag 18240 tccattgaaa agattcaagc tgccctgccc aatggaccat atgctgtcaa cctcatccat 18300 tctccttttg acagcaacct tgagaaggga aatgttgatc tcttcttgga aaagggtgtc 18360 acttttgttg aggcttcagc cttcatgacg ttgacaccgc aagttgtcag atacagagcg 18420 gctgggttga cgaggaatgc agatgggtca gtcaacataa ggaacagaat cattgggaaa 18480 gtctccagaa ctgagctggc tgaaatgttc atgcgtcctg cacctgagca tcttttgcag 18540 aagctcattg cttctgggga gatcaatcaa gaacaagctg agttggccag acgtgttcct 18600 gtggctgatg acattgcggt tgaagctgat tctggtggac acactgacaa ccgtccaatc 18660 catgtcattc tgcctctgat tatcaacctg agggataggc ttcacagaga gtgtggatac 18720 ccagcaaacc tgagggttcg tgttggtgct gggggtggca ttgggtgtcc ccaagctgcg 18780 ctggcaactt tcaacatggg tgcctctttc attgtcactg gaacagtgaa ccaagttgcc 18840 aaacaatctg ggacgtgtga caatgtgagg aaacagcttg cgaaagcaac ttactcagat 18900 gtttgcatgg caccggcagc tgacatgttt gaggaaggtg tgaaacttca agttttgaag 18960 aaaggaacaa tgttcccttc aagagccaac aaactctatg agttgttctg caagtatgat 19020 tcttttgagt ccatgcctcc agcagagctt gcgagggttg agaaaagaat cttcagtcgt 19080 gcattggaag aggtctggga tgagaccaag aacttctaca tcaaccgtct ccacaaccca 19140 gagaagatac agagggctga gagggacccc aaactgaaga tgtcactgtg tttcagatgg 19200 tatctgtccc tggcttcaag gtgggccaac actggagctt ctgacagagt catggactac 19260 caagtttggt gtggtcctgc cattggttca ttcaatgatt tcatcaaagg aacttacctt 19320 gatccggcag tggcaaatga gtacccgtgt gttgtgcaga tcaacaaaca gattcttaga 19380 ggagcgtgct tcttgaggcg tcttgaaata ctgagaaatg caaggctttc agatggtgca 19440 gctgcacttg tggcgagcat tgatgacaca tatgtccctg ctgagaagct gtgagtagtt 19500 agcttaatca cttaggtcac cagtatgaac taaaatgcat gtaggtgtaa gagctcatgg 19560 agagcatgga atattgtatc cgaccatgta acagtataat aactgagctc catctcactt 19620 cttctatgaa taaacaaagg atgttatgat atattaacac tctatctatg caccttattg 19680 ttctatgata aatttcctct tattattata aatcatctga atcgtgacgg cttatggaat 19740 gcttcaaata gtacaaaaac aaatgtgtac tataagactt tctaaacaat tctaacttta 19800 gcattgtgaa cgagacataa gtgttaagaa gacataacaa ttataatgga agaagtttgt 19860 ctccatttat atattatata ttacccactt atgtattata ttaggatgtt aaggagacat 19920 aacaattata aagagagaag tttgtatcca tttatatatt atatactacc catttatata 19980 ttatacttat ccacttattt aatgtcttta taaggtttga tccatgatat ttctaatatt 20040 ttagttgata tgtatatgaa aaggtactat ttgaactctc ttactctgta taaaggttgg 20100 atcatcctta aagtgggtct atttaatttt attgcttctt acagataaaa aaaaaattat 20160 gagttggttt gataaaatat tgaaggattt aaaataataa taaataataa ataacatata 20220 atatatgtat ataaatttat tataatataa catttatcta taaaaaagta aatattgtca 20280 taaatctata caatcgttta gccttgctgg aacgaatctc aattatttaa acgagagtaa 20340 acatatttga ctttttggtt atttaacaaa ttattattta acactatatg aaattttttt 20400 tttttatcag caaagaataa aattaaatta agaaggacaa tggtgtccca atccttatac 20460 aaccaacttc cacaagaaag tcaagtcaga gacaacaaaa aaacaagcaa aggaaatttt 20520 ttaatttgag ttgtcttgtt tgctgcataa tttatgcagt aaaacactac acataaccct 20580 tttagcagta gagcaatggt tgaccgtgtg cttagcttct tttattttat ttttttatca 20640 gcaaagaata aataaaataa aatgagacac ttcagggatg tttcaaccct tatacaaaac 20700 cccaaaaaca agtttcctag caccctacca acgaattcgc ggccgcttaa ttaatctgac 20760 ctgaccacta gacctgagtg agtacagatg tgctactagt gctagcctcg aggtcgacca 20820 acttttctat acaaagttgt ctagacctag gactaggcac tgagtgactc actgacctga 20880 ctgagtgact ggcggccgcc tcccagtatc attatagtga aagttttggc tctctcgccg 20940 gtggtttttt acctctattt aaaggggttt tccacctaaa aattctggta tcattctcac 21000 tttacttgtt actttaattt ctcataatct ttggttgaaa ttatcacgct tccgcacacg 21060 atatccctac aaatttatta tttgttaaac attttcaaac cgcataaaat tttatgaagt 21120 cccgtctatc tttaatgtag tctaacattt tcatattgaa atatataatt tacttaattt 21180 tagcgttggt agaaagcata atgatttatt cttattcttc ttcatataaa tgtttaatat 21240 acaatataaa caaattcttt accttaagaa ggatttccca ttttatattt taaaaatata 21300 tttatcaaat atttttcaac cacgtaaatc tcataataat aagttgtttc aaaagtaata 21360 aaatttaact ccataatttt tttattcgac tgatcttaaa gcaacaccca gtgacacaac 21420 tagccatttt tttctttgaa taaaaaaatc caattatcat tgtatttttt ttatacaatg 21480 aaaatttcac caaacaatga tttgtggtat ttctgaagca agtcatgtta tgcaaaattc 21540 tataattccc atttgacact acggaagtaa ctgaagatct gcttttacat gcgagacaca 21600 tcttctaaag taattttaat aatagttact atattcaaga tttcatatat caaatactca 21660 atattacttc taaaaaatta attagatata attaaaatat tactttttta attttaagtt 21720 taattgttga atttgtgact attgatttat tattctacta tgtttaaatt gttttataga 21780 tagtttaaag taaatataag taatgtagta gagtgttaga gtgttaccct aaaccataaa 21840 ctataagatt tatggtggac taattttcat atatttctta ttgcttttac cttttcttgg 21900 tatgtaagtc cgtaactgga attactgtgg gttgccatga cactctgtgg tcttttggtt 21960 catgcatgga tcttgcgcaa gaaaaagaca aagaacaaag aaaaaagaca aaacagagag 22020 acaaaacgca atcacacaac caactcaaat tagtcactgg ctgatcaaga tcgccgcgtc 22080 catgtatgtc taaatgccat gcaaagcaac acgtgcttaa catgcacttt aaatggctca 22140 cccatctcaa cccacacaca aacacattgc ctttttcttc atcatcacca caaccacctg 22200 tatatattca ttctcttccg ccacctcaat ttcttcactt caacacacgt caacctgcat 22260 atgcgtgtca tcccatgccc aaatctccat gcatgttcca accaccttct ctcttatata 22320 atacctataa atacctctaa tatcactcac ttctttcatc atccatccat ccagagtact 22380 actactctac tactataata ccccaaccca actcatattc aatactactc taggatccac 22440 aaccaaaaat ggcgcttcgt gtcaagacca acaagaagcc atgttgggag atgaccaagg 22500 aggaactgac ctctggcaaa actgaggtgt tcaactatga ggaactcctg gagtttgcag 22560 agggtgacat agctaaggtt tttggtccgg agtttgctgt catagacaag taccccagaa 22620 gggtgaggct gcctgctcgt gaatacctgc ttgtgacaag ggtcacactc atggatgccg 22680 aggtcaacaa ctacagagtt ggtgccagaa tggtcactga gtatgatctt ccagtgaatg 22740 gcgaattgtc tgagggaggt gattgtccct gggcagttct ggttgagagt ggacagtgcg 22800 atttgatgct gatatcatac atgggaattg atttccagaa ccaaggtgac agagtttacc 22860 gtctgctcaa caccacgttg acattctatg gggttgccca tgagggagag acccttgagt 22920 atgacatcag agtgaccggt tttgccaaac gtttggatgg tggcatctcc atgttcttct 22980 ttgagtatga ctgctatgtc aatgggaggc ttctcattga gatgagagat ggctgtgctg 23040 gcttcttcac aaatgaggag cttgatgcgg gaaagggtgt ggtgttcaca agaggggatt 23100 tggctgccag agctaagatt ccaaagcaag atgtttctcc ctatgctgtc gctccctgcc 23160 ttcacaaaac aaaactcaac gaaaaggaga tgcagacctt ggttgacaaa gattgggctt 23220 ccgtttttgg ctcaaagaat ggcatgccgg agatcaacta caaactctgc gcacgtaaga 23280 tgctcatgat tgatagagtc accagcattg accataaggg aggtgtgtac ggtttgggtc 23340 agttggttgg tgagaaaatc ttggagaggg accattggta cttcccatgc cattttgtta 23400 aggatcaagt catggctgga tctcttgtca gcgacggttg ttcacaaatg ctcaagatgt 23460 acatgatctg gctcggtttg caccttacca ccggaccatt tgacttcaga cccgtcaacg 23520 gacacccgaa caaagttagg tgtaggggtc agatatctcc tcataaggga aagttggtct 23580 acgtcatgga gatcaaagag atgggattcg acgaggataa cgatccgtac gccattgctg 23640 acgtcaacat cattgacgtt gatttcgaaa agggtcaaga tttcagcctt gatagaatca 23700 gcgactacgg aaagggggat cttaacaaaa agatcgtcgt ggacttcaag ggcattgctc 23760 tcaagatgca gaagagatcc acgaacaaga acccctccaa agttcagccc gtttttgcta 23820 acggtgcagc cactgtggga ccggaagcgt ccaaggccag ctctggtgcc agtgctagtg 23880 cttctgctgc ccctgcgaag cctgctttct cagcagatgt tcttgcgcca aagccagttg 23940 cacttcctga acatatcctc aagggtgacg ctctcgctcc gaaagaaatg tcctggcatc 24000 ccatgtcaaa acttcctggc aacccgacgc cgttcttctc ccctagttct taccctccga 24060 gggcaatttg cttcatccct ttccctggca acccacttga caacaattgc aaagctggag 24120 aaatgcctct gaactggtac aacatgtcag agttcatgtg tggaaaagtt agcaactgct 24180 tgggaccaga gttcgcaaga tttgataagt caaatacgag cagaagccct gcttttgatt 24240 tggctcttgt gactagagtt gttgaagtca caaacatgga acatggcaag ttcctgaatg 24300 ttgattgcaa tcccagcaaa ggcacaatgg tgggggagtt tgactgtcct caagatgcgt 24360 ggttctttga tggttcttgc aatgatgggc acatgcctta ttccatcatc atggaaatcg 24420 gacttcagac atccggtgtt ctcaccagtg tgttgaaagc accactgacg atggacaaag 24480 atgacattct ctttaggaac cttgatgcaa gtgctgaaat ggtgaggcca gatgtggatg 24540 tgagggggaa aaccataagg aatgtgacca agtgcactgg atatgcaatg ttgggaaaga 24600 tggggattca cagattcacg tttgagttgt ctgttgatgg cgtggttttc tacaaaggaa 24660 gcacttcctt tggatggttc actccagagg tgtttgctca gcaagctgga cttgacaatg 24720 ggaaaaagac ggagccttgg tgcaaaacta acaacacgag tgtgagaaga gttgaaatcg 24780 catcagccaa aggcaaagaa cagctgactg agaaacttcc agatgcaact aatgctcaag 24840 ttctcagaag gtcagaacag tgtgagtatt tggattacct caacattgct cctgattctg 24900 ggctgcatgg gaaaggatat gcgcatggac acaaagatgt taacccacaa gactggttct 24960 tctcttgtca cttttggttt gatcctgtga tgcctggatc tttgggaatt gagtcaatgt 25020 tccagctcat tgaggcattt gcggtggacc agaacatacc gggagaatac aatgtgagca 25080 atccgacctt tgcccatgcc cctggaaaaa cggcgtggaa gtaccgtggt caattgacac 25140 caaagaaccg tgcgatggac tgtgaggtgc acatagtttc aatcacagcc tctcctgaaa 25200 atggaggcta cgttgacata gtggcagatg gagcactttg ggttgatggt ctccgtgtgt 25260 atgaagccaa agaactcaga gttcgtgttg tcagtgcaaa acctcaagcc attccggatg 25320 ttcaacaaca gccacccagc gcaaaagcgg accctgggaa aactggagtt gcactcagtc 25380 cgactcagtt gagagatgtc ctgcttgaag tggacaatcc attgtatctt ggtgttgaaa 25440 actccaattt ggtgcagttt gaatccaaac ctgcaacttc ttcaaggatt gtcagtatca 25500 aaccatgctc cataagtgat cttggtgaca agtccttcat ggaaacgtac aatgtgtcag 25560 cacctctgta cactggagca atggccaagg gaattgcgtc tgcggacctt gtcattgctg 25620 ctgggaagag gaagatattg ggttcttttg gagcgggtgg gcttccaatg caccatgtga 25680 gggcagcttt ggagaagata caagcggctc tgcctcaagg accatatgct gtgaacctca 25740 ttcattcacc ttttgacagc aacttggaaa aggggaatgt tgatctcttc ctcgaaaagg 25800 gcgttactgt ggtggaggct tctgcattca tgaccctcac tccccaagtt gttcgttacc 25860 gtgcagccgg actctcaagg aacgctgatg ggagtgtcaa catcagaaac cgtatcattg 25920 gaaaagtgtc cagaacagaa ctcgccgaaa tgttcatcag accggctccg gaacacctcc 25980 ttgagaaact catagcttct ggtgagataa cacaagaaca agccgaactt gcccgtcgtg 26040 ttcctgtcgc agacgacatc gctgttgaag ctgattctgg tggtcacact gacaaccgtc 26100 ccatccacgt catcttgcct ttgatcatca accttaggaa cagactgcac agagagtgtg 26160 gttatcctgc gcaccttaga gtcagagttg gagctggagg cggtgttggc tgcccacaag 26220 ctgctgcggc tgccctcaca atgggagctg ccttcatagt gactgggact gtcaatcaag 26280 ttgctaaaca gtccgggaca tgtgacaacg tgagaaaaca actcagtcaa gccacctaca 26340 gtgacatttg catggctcca gcagctgata tgtttgagga aggtgtgaaa ctccaagttc 26400 tcaagaaggg aacaatgttc ccatcaagag ctaacaaatt gtacgaactc ttttgcaagt 26460 acgactcttt cgattcaatg cctcctgctg aacttgaaag gatcgagaag aggatcttca 26520 agagggcact tcaagaggtc tgggaagaga ccaaggactt ttacatcaat ggtttgaaga 26580 atccagagaa gatacagaga gcggaacatg accccaaact caagatgtct ttgtgtttca 26640 gatggtatct tggtcttgca tcacgttggg ccaacatggg tgctcctgat cgtgtcatgg 26700 actatcaagt ctggtgtggt ccagccattg gagccttcaa tgacttcatc aaaggaacat 26760 atcttgatcc tgctgtctct aatgagtacc catgtgttgt ccagatcaac cttcagattt 26820 tgcgtggtgc atgttacctg aggcgtctta atgctctgag aaatgatccg aggattgatc 26880 ttgagactga agatgctgcc tttgtctatg agccaacgaa tgcgctttga gtagttagct 26940 taatcactta ggtcaccagt atgaactaaa atgcatgtag gtgtaagagc tcatggagag 27000 catggaatat tgtatccgac catgtaacag tataataact gagctccatc tcacttcttc 27060 tatgaataaa caaaggatgt tatgatatat taacactcta tctatgcacc ttattgttct 27120 atgataaatt tcctcttatt attataaatc atctgaatcg tgacggctta tggaatgctt 27180 caaatagtac aaaaacaaat gtgtactata agactttcta aacaattcta actttagcat 27240 tgtgaacgag acataagtgt taagaagaca taacaattat aatggaagaa gtttgtctcc 27300 atttatatat tatatattac ccacttatgt attatattag gatgttaagg agacataaca 27360 attataaaga gagaagtttg tatccattta tatattatat actacccatt tatatattat 27420 acttatccac ttatttaatg tctttataag gtttgatcca tgatatttct aatattttag 27480 ttgatatgta tatgaaaagg tactatttga actctcttac tctgtataaa ggttggatca 27540 tccttaaagt gggtctattt aattttattg cttcttacag ataaaaaaaa aattatgagt 27600 tggtttgata aaatattgaa ggatttaaaa taataataaa taataaataa catataatat 27660 atgtatataa atttattata atataacatt tatctataaa aaagtaaata ttgtcataaa 27720 tctatacaat cgtttagcct tgctggaacg aatctcaatt atttaaacga gagtaaacat 27780 atttgacttt ttggttattt aacaaattat tatttaacac tatatgaaat tttttttttt 27840 tatcagcaaa gaataaaatt aaattaagaa ggacaatggt gtcccaatcc ttatacaacc 27900 aacttccaca agaaagtcaa gtcagagaca acaaaaaaac aagcaaagga aattttttaa 27960 tttgagttgt cttgtttgct gcataattta tgcagtaaaa cactacacat aaccctttta 28020 gcagtagagc aatggttgac cgtgtgctta gcttctttta ttttattttt ttatcagcaa 28080 agaataaata aaataaaatg agacacttca gggatgtttc aacccttata caaaacccca 28140 aaaacaagtt tcctagcacc ctaccaacga attcgcggcc gcttaattaa tcacgagtgg 28200 atgactgtca cgactgactc atgactgact actagtgcta gcctcgaggt cgacacaact 28260 ttgtataata aagttgtcta gacctaggag ctgtgctggg taactcacat gagtcactgg 28320 tcacatcagc ggccgcctcc cagtatcatt atagtgaaag ttttggctct ctcgccggtg 28380 gttttttacc tctatttaaa ggggttttcc acctaaaaat tctggtatca ttctcacttt 28440 acttgttact ttaatttctc ataatctttg gttgaaatta tcacgcttcc gcacacgata 28500 tccctacaaa tttattattt gttaaacatt ttcaaaccgc ataaaatttt atgaagtccc 28560 gtctatcttt aatgtagtct aacattttca tattgaaata tataatttac ttaattttag 28620 cgttggtaga aagcataatg atttattctt attcttcttc atataaatgt ttaatataca 28680 atataaacaa attctttacc ttaagaagga tttcccattt tatattttaa aaatatattt 28740 atcaaatatt tttcaaccac gtaaatctca taataataag ttgtttcaaa agtaataaaa 28800 tttaactcca taattttttt attcgactga tcttaaagca acacccagtg acacaactag 28860 ccattttttt ctttgaataa aaaaatccaa ttatcattgt atttttttta tacaatgaaa 28920 atttcaccaa acaatgattt gtggtatttc tgaagcaagt catgttatgc aaaattctat 28980 aattcccatt tgacactacg gaagtaactg aagatctgct tttacatgcg agacacatct 29040 tctaaagtaa ttttaataat agttactata ttcaagattt catatatcaa atactcaata 29100 ttacttctaa aaaattaatt agatataatt aaaatattac ttttttaatt ttaagtttaa 29160 ttgttgaatt tgtgactatt gatttattat tctactatgt ttaaattgtt ttatagatag 29220 tttaaagtaa atataagtaa tgtagtagag tgttagagtg ttaccctaaa ccataaacta 29280 taagatttat ggtggactaa ttttcatata tttcttattg cttttacctt ttcttggtat 29340 gtaagtccgt aactggaatt actgtgggtt gccatgacac tctgtggtct tttggttcat 29400 gcatggatct tgcgcaagaa aaagacaaag aacaaagaaa aaagacaaaa cagagagaca 29460 aaacgcaatc acacaaccaa ctcaaattag tcactggctg atcaagatcg ccgcgtccat 29520 gtatgtctaa atgccatgca aagcaacacg tgcttaacat gcactttaaa tggctcaccc 29580 atctcaaccc acacacaaac acattgcctt tttcttcatc atcaccacaa ccacctgtat 29640 atattcattc tcttccgcca cctcaatttc ttcacttcaa cacacgtcaa cctgcatatg 29700 cgtgtcatcc catgcccaaa tctccatgca tgttccaacc accttctctc ttatataata 29760 cctataaata cctctaatat cactcacttc tttcatcatc catccatcca gagtactact 29820 actctactac tataataccc caacccaact catattcaat actactctag gatccacaac 29880 caaaaatgct tcagcacact tggcttccga agcctcccaa tctgaccctc ttgtcagatg 29940 aggttcatct ctggaggatt cctcttgacc agcctgagtc acaacttcaa gaccttgctg 30000 ccaccctgag cagtgatgaa ttggcgaggg caaacagatt ctactttcca gaacacagaa 30060 ggcgtttcac tgctgggaga ggcatcctca gatccatctt gggtggatac ttgggagtgg 30120 aaccgggtca agtcaagttt gattatgagt cccgtgggaa accgatcctt ggtgacagat 30180 ttgctgagag tggactcctg ttcaacttgt ctcacagcca gaaccttgcc ttgtgtgctg 30240 tcaactacac gcgtcaaata ggcattgatc ttgaatatct gcgtccaaca tctgacttgg 30300 agtctcttgc aaagaggttc tttctcccaa gagaatatga actcttgagg tcactccctg 30360 atgagcagaa acagaagatt ttctttcgtt actggacttg caaagaggct tatctcaaag 30420 caacgggaga tggaatagcc aaacttgaag agatcgagat agcactcacc ccaacagaac 30480 ctgccaagct ccaaacagct cctgcgtggt ctctgttgga gcttgtgcca gatgacaatt 30540 gtgttgcagc tgtggctgtt gcgggttttg gttggcagcc caagttctgg cattactgag 30600 tagttagctt aatcacttag gtcaccagta tgaactaaaa tgcatgtagg tgtaagagct 30660 catggagagc atggaatatt gtatccgacc atgtaacagt ataataactg agctccatct 30720 cacttcttct atgaataaac aaaggatgtt atgatatatt aacactctat ctatgcacct 30780 tattgttcta tgataaattt cctcttatta ttataaatca tctgaatcgt gacggcttat 30840 ggaatgcttc aaatagtaca aaaacaaatg tgtactataa gactttctaa acaattctaa 30900 ctttagcatt gtgaacgaga cataagtgtt aagaagacat aacaattata atggaagaag 30960 tttgtctcca tttatatatt atatattacc cacttatgta ttatattagg atgttaagga 31020 gacataacaa ttataaagag agaagtttgt atccatttat atattatata ctacccattt 31080 atatattata cttatccact tatttaatgt ctttataagg tttgatccat gatatttcta 31140 atattttagt tgatatgtat atgaaaaggt actatttgaa ctctcttact ctgtataaag 31200 gttggatcat ccttaaagtg ggtctattta attttattgc ttcttacaga taaaaaaaaa 31260 attatgagtt ggtttgataa aatattgaag gatttaaaat aataataaat aataaataac 31320 atataatata tgtatataaa tttattataa tataacattt atctataaaa aagtaaatat 31380 tgtcataaat ctatacaatc gtttagcctt gctggaacga atctcaatta tttaaacgag 31440 agtaaacata tttgactttt tggttattta acaaattatt atttaacact atatgaaatt 31500 tttttttttt atcagcaaag aataaaatta aattaagaag gacaatggtg tcccaatcct 31560 tatacaacca acttccacaa gaaagtcaag tcagagacaa caaaaaaaca agcaaaggaa 31620 attttttaat ttgagttgtc ttgtttgctg cataatttat gcagtaaaac actacacata 31680 acccttttag cagtagagca atggttgacc gtgtgcttag cttcttttat tttatttttt 31740 tatcagcaaa gaataaataa aataaaatga gacacttcag ggatgtttca acccttatac 31800 aaaaccccaa aaacaagttt cctagcaccc taccaacgaa ttcgcggccg cttaattaac 31860 tactgtcact gaggccgtag acgagtacgg actgatctaa ctagtgctag cctcgaggtc 31920 gacacccagc tttcttgtac aaagtggttg cggccgctta attaaattta aattcaatta 31980 atgcaatctt gattttcaac aacgaaggta atggcgtaaa agaaaaaatg tatgttattg 32040 tattgatctt tcatgatgtt gaagcgtgcc ataatatgat gatgtataat taaaatatta 32100 actgtcgcat tttattgaaa tggcactgtt atttcaacca tatctttgat tctgttacat 32160 gacacgactg caagaagtaa ataatagacg ccgttgttaa agaattgcta tcatatgtgc 32220 ctaactagag ggaatttgag cgtcagacct aatcaaatat tacaaaatat ctcactctgt 32280 cgccagcaat ggtgtaatca gcgcagacaa atggcgtaaa gatcgcggaa aaacctcccc 32340 gagtggcatg atagctgcct ctgtattgct gatttagtca gccttatttg acttaagggt 32400 gccctcgtta gtgacaaatt gctttcaagg agacagccat gccccacact ttgttgaaaa 32460 acaaattgcc tttggggaga cggtaaagcc agttgctctt caataaggaa tgtcgaggag 32520 gcaatgtaac cgcctctggt agtacacttc tctaatccaa aaatcaattt gtattcaaga 32580 taccgcaaaa aacttatggt ttaaaccctg caggactagt ccagaaggta attatccaag 32640 atgtagcatc aagaatccaa tgtttacggg aaaaactatg gaagtattat gtaagctcag 32700 caagaagcag atcaatatgc ggcacatatg caacctatgt tcaaaaatga agaatgtaca 32760 gatacaagat cctatactgc cagaatacga agaagaatac gtagaaattg aaaaagaaga 32820 accaggcgaa gaaaagaatc ttgaagacgt aagcactgac gacaacaatg aaaagaagaa 32880 gataaggtcg gtgattgtga aagagacata gaggacacat gtaaggtgga aaatgtaagg 32940 gcggaaagta accttatcac aaaggaatct tatcccccac tacttatcct tttatatttt 33000 tccgtgtcat ttttgccctt gagttttcct atataaggaa ccaagttcgg catttgtgaa 33060 aacaagaaaa aatttggtgt aagctatttt ctttgaagta ctgaggatac aacttcagag 33120 aaatttgtaa gtttgtaggt accagatctg gatcccaaac catgtctccg gagaggagac 33180 cagttgagat taggccagct acagcagctg atatggccgc ggtttgtgat atcgttaacc 33240 attacattga gacgtctaca gtgaacttta ggacagagcc acaaacacca caagagtgga 33300 ttgatgatct agagaggttg caagatagat acccttggtt ggttgctgag gttgagggtg 33360 ttgtggctgg tattgcttac gctgggccct ggaaggctag gaacgcttac gattggacag 33420 ttgagagtac tgtttacgtg tcacataggc atcaaaggtt gggcctagga tctacattgt 33480 acacacattt gcttaagtct atggaggcgc aaggttttaa gtctgtggtt gctgttatag 33540 gccttccaaa cgatccatct gttaggttgc atgaggcttt gggatacaca gcccggggta 33600 cattgcgcgc agctggatac aagcatggtg gatggcatga tgttggtttt tggcaaaggg 33660 attttgagtt gccagctcct ccaaggccag ttaggccagt tacccaaatc tgagtagtta 33720 gcttaatcac ctagagctcg atcggcggca atagcttctt agcgccatcc cgggttgatc 33780 ctatctgtgt tgaaatagtt gcggtgggca aggctctctt tcagaaagac aggcggccaa 33840 aggaacccaa ggtgaggtgg gctatggctc tcagttcctt gtggaagcgc ttggtctaag 33900 gtgcagaggt gttagcggga tgaagcaaaa gtgtccgatt gtaacaagat atgttgatcc 33960 tacgtaagga tattaaagta tgtattcatc actaatataa tcagtgtatt ccaatatgta 34020 ctacgatttc caatgtcttt attgtcgccg tatgtaatcg gcgtcacaaa ataatccccg 34080 gtgactttct tttaatccag gatgaaataa tatgttatta taatttttgc gatttggtcc 34140 gttataggaa ttgaagtgtg cttgaggtcg gtcgccacca ctcccatttc ataattttac 34200 atgtatttga aaaataaaaa tttatggtat tcaatttaaa cacgtatact tgtaaagaat 34260 gatatcttga aagaaatata gtttaaatat ttattgataa aataacaagt caggtattat 34320 agtccaagca aaaacataaa tttattgatg caagtttaaa ttcagaaata tttcaataac 34380 tgattatatc agctggtaca ttgccgtaga tgaaagactg agtgcgatat tatggtgtaa 34440 tacataggaa ttcgtttaaa cgatctgcgt ctaattttcg gtccaacttg cacaggaaag 34500 acgtcgaccg cggtagctct tgcccagcag actgggcttc cagtcctttc gctcgatcgg 34560 gtccaatgtt gtcctcagct gtgaaccgga agcggacgac caacagtgga agaactgaaa 34620 ggaacgagcc gtctatacct tgatgatcgg cctctggtga agggtatcat cgcagccaag 34680 caagctcatg aaaggctgat gggggaggtg tataattatg aggcccacgg cgggcttatt 34740 ctttagggag gatctatctc gttgctcaag tgcatggcgc aaagcagtta ttggagtgcg 34800 gattttcgtt ggcatattat tcgccacgag ttagcagacg aagagacctt catgaacgtg 34860 gccaaggcca gagttaagca gatgttacgc cctgctgcag gcctttctat tatccaatag 34920 ttggttgatc tttggaaaga gcctcggctg aggcccatac tgaaagagat cgatggatat 34980 cgatatgcca tgttgtttgc tagccagaac cagatcacat ccgatatgct attgcagctt 35040 gacgcagata tggaggataa gttgattcat gggatcgctc aggagtagct catccatgca 35100 cgccgacaag aacagaaatt ccgtcgagtt aacgcagccg cttacgacgg attcgaaggt 35160 catccattcg gaatgtatta gtttgcacca gctccgcgtc acacctgtct tcatttgaat 35220 aagatgttag caattgtttt tagctttgtc ttgttgtggc agggcggcaa gtgcttcaga 35280 catcattctg ttttcaaatt ttatgctgga gaacagcttc ttaattcctt tggaaataat 35340 agactgcgtc ttaaaattca gatgtctgga tatagatatg attgtaaaat aacctattta 35400 agtgtcattt agaacataag ttttatgaat gttcttccat tttcgtcatc gaacgaataa 35460 gagtaaatac acctttttta acattacaaa taagttctta tacgttgttt atacaccggg 35520 aatcatttcc attattttcg cgcaaaagtc acggatattc gtgaaagcga cataaactgc 35580 gaaatttgcg gggagtgtct tgagtttgcc tcgaggctag cgcatgcaca tagacacaca 35640 catcatctca ttgatgcttg gtaataattg tcattagatt gtttttatgc atagatgcac 35700 tcgaaatcag ccaattttag acaagtatca aacggatgtg acttcagtac attaaaaacg 35760 tccgcaatgt gttattaagt tgtctaagcg tcaatttgat ttacaattga atatatcctg 35820 ccccagccag ccaacagctc gatttacaat tgaatatatc ctgccggccg gcccacgcgt 35880 gtcgaggaat tctgatctgg cccccatttg gacgtgaatg tagacacgtc gaaataaaga 35940 tttccgaatt agaataattt gtttattgct ttcgcctata aatacgacgg atcgtaattt 36000 gtcgttttat caaaatgtac tttcatttta taataacgct gcggacatct acatttttga 36060 attgaaaaaa aattggtaat tactctttct ttttctccat attgaccatc atactcattg 36120 ctgatccatg tagatttccc ggacatgaag ccatttacaa ttgaatatat cctgccgccg 36180 ctgccgcttt gcacccggtg gagcttgcat gttggtttct acgcagaact gagccggtta 36240 ggcagataat ttccattgag aactgagcca tgtgcacctt ccccccaaca cggtgagcga 36300 cggggcaacg gagtgatcca catgggactt ttaaacatca tccgtcggat ggcgttgcga 36360 gagaagcagt cgatccgtga gatcagccga cgcaccgggc aggcgcgcaa cacgatcgca 36420 aagtatttga acgcaggtac aatcgagccg acgttcacgc ggaacgacca agcaagcttg 36480 gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg 36540 aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg 36600 cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa 36660 agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct 36720 ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc 36780 actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa 36840 gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc 36900 gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa 36960 tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac 37020 gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg 37080 gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac 37140 ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtcggaaagg gtcgacggat 37200 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc 37260 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat 37320 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt 37380 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg 37440 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac 37500 caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga 37560 ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg 37620 ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg 37680 cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc 37740 gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga 37800 gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc 37860 gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga 37920 agagcgactt cgcggagctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga 37980 aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca 38040 ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg 38100 caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac 38160 tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc 38220 gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga 38280 tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg 38340 agcgttcgcg tcgtctcgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca 38400 cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg 38460 tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc 38520 agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca 38580 cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa 38640 acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc 38700 gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta 38760 tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca 38820 atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg 38880 gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg 38940 tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg 39000 tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc 39060 cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc 39120 tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc 39180 aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca 39240 atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt 39300 cagcagccag cgctttactg gcatttcagg aacaagcggg cactgctcga cgcacttgct 39360 tcgctcagta tcgctcggga cgcacggcgc gctctacgaa ctgccgataa acagaggatt 39420 aaaattgaca attgtgatta aggctcagat tcgacggctt ggagcggccg acgtgcagga 39480 tttccgcgag atccgattgt cggccctgaa gaaagctcca gagatgttcg ggtccgttta 39540 cgagcacgag gagaaaaagc ccatggaggc gttcgctgaa cggttgcgag atgccgtggc 39600 attcggcgcc tacatcgacg gcgagatcat tgggctgtcg gtcttcaaac aggaggacgg 39660 ccccaaggac gctcacaagg cgcatctgtc cggcgttttc gtggagcccg aacagcgagg 39720 ccgaggggtc gccggtatgc tgctgcgggc gttgccggcg ggtttattgc tcgtgatgat 39780 cgtccgacag attccaacgg gaatctggtg gatgcgcatc ttcatcctcg gcgcacttaa 39840 tatttcgcta ttctggagct tgttgtttat ttcggtctac cgcctgccgg gcggggtcgc 39900 ggcgacggta ggcgctgtgc agccgctgat ggtcgtgttc atctctgccg ctctgctagg 39960 tagcccgata cgattgatgg cggtcctggg ggctatttgc ggaactgcgg gcgtggcgct 40020 gttggtgttg acaccaaacg cagcgctaga tcctgtcggc gtcgcagcgg gcctggcggg 40080 ggcggtttcc atggcgttcg gaaccgtgct gacccgcaag tggcaacctc ccgtgcctct 40140 gctcaccttt accgcctggc aactggcggc cggaggactt ctgctcgttc cagtagcttt 40200 agtgtttgat ccgccaatcc cgatgcctac aggaaccaat gttctcggcc tggcgtggct 40260 cggcctgatc ggagcgggtt taacctactt cctttggttc cgggggatct cgcgactcga 40320 acctacagtt gtttccttac tgggctttct cagcccccga gcgcttagtg ggaatttgta 40380 ccccttatcg aaccgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct 40440 tcgcggcgcg gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta 40500 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 40560 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 40620 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 40680 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 40740 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 40800 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 40860 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 40920 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 40980 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 41040 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 41100 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag 41160 gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt 41220 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca 41280 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gagagctggg gaagactatg 41340 cgcgatctgt tgaaggtggt tctaagcctc gtcttgcgat ggcatttcga tccattccca 41400 ttccgcgctc aagatggctt cccctcggca gttcatcagg gctaaatcaa tctagccgac 41460 ttgtccggtg aaatgggctg cactccaaca gaaacaatca aacaaacata cacagcgact 41520 tattcacacg agctcaaatt acaacggtat atatcctgcc agtcagcatc atcacaccaa 41580 aagttaggcc cgaatagttt gaaattagaa agctcgcaat tgaggtctac aggccaaatt 41640 cgctcttagc cgtacaatat tactcaccgg atcctaaccg gtttaattaa ggcgcgccat 41700 gcccgggcaa gcggccgcac aagttt 41726 <210> 53 <211> 36797 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9338 <400> 53 cttgtacaaa gtggttgcgg ccgcttaatt aaatttaaat tcaattaatg caatcttgat 60 tttcaacaac gaaggtaatg gcgtaaaaga aaaaatgtat gttattgtat tgatctttca 120 tgatgttgaa gcgtgccata atatgatgat gtataattaa aatattaact gtcgcatttt 180 attgaaatgg cactgttatt tcaaccatat ctttgattct gttacatgac acgactgcaa 240 gaagtaaata atagacgccg ttgttaaaga attgctatca tatgtgccta actagaggga 300 atttgagcgt cagacctaat caaatattac aaaatatctc actctgtcgc cagcaatggt 360 gtaatcagcg cagacaaatg gcgtaaagat cgcggaaaaa cctccccgag tggcatgata 420 gctgcctctg tattgctgat ttagtcagcc ttatttgact taagggtgcc ctcgttagtg 480 acaaattgct ttcaaggaga cagccatgcc ccacactttg ttgaaaaaca aattgccttt 540 ggggagacgg taaagccagt tgctcttcaa taaggaatgt cgaggaggca atgtaaccgc 600 ctctggtagt acacttctct aatccaaaaa tcaatttgta ttcaagatac cgcaaaaaac 660 ttatggttta aaccctgcag gactagtcca gaaggtaatt atccaagatg tagcatcaag 720 aatccaatgt ttacgggaaa aactatggaa gtattatgta agctcagcaa gaagcagatc 780 aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat acaagatcct 840 atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc aggcgaagaa 900 aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat aaggtcggtg 960 attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg gaaagtaacc 1020 ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc gtgtcatttt 1080 tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac aagaaaaaat 1140 ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa tttgtaagtt 1200 tgtaggtacc agatctggat cccaaaccat gtctccggag aggagaccag ttgagattag 1260 gccagctaca gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac 1320 gtctacagtg aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga 1380 gaggttgcaa gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat 1440 tgcttacgct gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt 1500 ttacgtgtca cataggcatc aaaggttggg cctaggatct acattgtaca cacatttgct 1560 taagtctatg gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga 1620 tccatctgtt aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc 1680 tggatacaag catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc 1740 agctcctcca aggccagtta ggccagttac ccaaatctga gtagttagct taatcaccta 1800 gagctcgatc ggcggcaata gcttcttagc gccatcccgg gttgatccta tctgtgttga 1860 aatagttgcg gtgggcaagg ctctctttca gaaagacagg cggccaaagg aacccaaggt 1920 gaggtgggct atggctctca gttccttgtg gaagcgcttg gtctaaggtg cagaggtgtt 1980 agcgggatga agcaaaagtg tccgattgta acaagatatg ttgatcctac gtaaggatat 2040 taaagtatgt attcatcact aatataatca gtgtattcca atatgtacta cgatttccaa 2100 tgtctttatt gtcgccgtat gtaatcggcg tcacaaaata atccccggtg actttctttt 2160 aatccaggat gaaataatat gttattataa tttttgcgat ttggtccgtt ataggaattg 2220 aagtgtgctt gaggtcggtc gccaccactc ccatttcata attttacatg tatttgaaaa 2280 ataaaaattt atggtattca atttaaacac gtatacttgt aaagaatgat atcttgaaag 2340 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa 2400 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc 2460 tggtacattg ccgtagatga aagactgagt gcgatattat ggtgtaatac ataggaattc 2520 gtttaaacga tctgcgtcta attttcggtc caacttgcac aggaaagacg tcgaccgcgg 2580 tagctcttgc ccagcagact gggcttccag tcctttcgct cgatcgggtc caatgttgtc 2640 ctcagctgtg aaccggaagc ggacgaccaa cagtggaaga actgaaagga acgagccgtc 2700 tataccttga tgatcggcct ctggtgaagg gtatcatcgc agccaagcaa gctcatgaaa 2760 ggctgatggg ggaggtgtat aattatgagg cccacggcgg gcttattctt tagggaggat 2820 ctatctcgtt gctcaagtgc atggcgcaaa gcagttattg gagtgcggat tttcgttggc 2880 atattattcg ccacgagtta gcagacgaag agaccttcat gaacgtggcc aaggccagag 2940 ttaagcagat gttacgccct gctgcaggcc tttctattat ccaatagttg gttgatcttt 3000 ggaaagagcc tcggctgagg cccatactga aagagatcga tggatatcga tatgccatgt 3060 tgtttgctag ccagaaccag atcacatccg atatgctatt gcagcttgac gcagatatgg 3120 aggataagtt gattcatggg atcgctcagg agtagctcat ccatgcacgc cgacaagaac 3180 agaaattccg tcgagttaac gcagccgctt acgacggatt cgaaggtcat ccattcggaa 3240 tgtattagtt tgcaccagct ccgcgtcaca cctgtcttca tttgaataag atgttagcaa 3300 ttgtttttag ctttgtcttg ttgtggcagg gcggcaagtg cttcagacat cattctgttt 3360 tcaaatttta tgctggagaa cagcttctta attcctttgg aaataataga ctgcgtctta 3420 aaattcagat gtctggatat agatatgatt gtaaaataac ctatttaagt gtcatttaga 3480 acataagttt tatgaatgtt cttccatttt cgtcatcgaa cgaataagag taaatacacc 3540 ttttttaaca ttacaaataa gttcttatac gttgtttata caccgggaat catttccatt 3600 attttcgcgc aaaagtcacg gatattcgtg aaagcgacat aaactgcgaa atttgcgggg 3660 agtgtcttga gtttgcctcg aggctagcgc atgcacatag acacacacat catctcattg 3720 atgcttggta ataattgtca ttagattgtt tttatgcata gatgcactcg aaatcagcca 3780 attttagaca agtatcaaac ggatgtgact tcagtacatt aaaaacgtcc gcaatgtgtt 3840 attaagttgt ctaagcgtca atttgattta caattgaata tatcctgccc cagccagcca 3900 acagctcgat ttacaattga atatatcctg ccggccggcc cacgcgtgtc gaggaattct 3960 gatctggccc ccatttggac gtgaatgtag acacgtcgaa ataaagattt ccgaattaga 4020 ataatttgtt tattgctttc gcctataaat acgacggatc gtaatttgtc gttttatcaa 4080 aatgtacttt cattttataa taacgctgcg gacatctaca tttttgaatt gaaaaaaaat 4140 tggtaattac tctttctttt tctccatatt gaccatcata ctcattgctg atccatgtag 4200 atttcccgga catgaagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4260 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4320 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4380 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4440 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 4500 caggtacaat cgagccgacg ttcacgcgga acgaccaagc aagcttggct gccatttttg 4560 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 4620 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 4680 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 4740 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 4800 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 4860 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 4920 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 4980 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 5040 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 5100 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 5160 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 5220 gcggcgaggg caaccagccc ggtgagcgtc ggaaagggtc gacggatctt ttccgctgca 5280 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 5340 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 5400 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 5460 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc 5520 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc 5580 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg 5640 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg 5700 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc 5760 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat 5820 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc 5880 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg 5940 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc 6000 ggagctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 6060 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 6120 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 6180 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 6240 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 6300 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 6360 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 6420 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 6480 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 6540 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 6600 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 6660 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 6720 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 6780 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 6840 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 6900 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 6960 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 7020 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 7080 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 7140 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 7200 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 7260 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 7320 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 7380 tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 7440 ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 7500 gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc 7560 cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag 7620 aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac 7680 atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct 7740 cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc 7800 ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt 7860 ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc 7920 tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc 7980 gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga 8040 ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca 8100 ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg 8160 gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc 8220 gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg 8280 ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga 8340 gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt 8400 tccttactgg gctttctcag cccccgagcg cttagtggga atttgtaccc cttatcgaac 8460 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct 8520 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 8580 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 8640 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 8700 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 8760 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 8820 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 8880 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 8940 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 9000 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 9060 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 9120 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 9180 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac 9240 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag 9300 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 9360 tcgcggcgcg gcttaactca agcgttagag agctggggaa gactatgcgc gatctgttga 9420 aggtggttct aagcctcgtc ttgcgatggc atttcgatcc attcccattc cgcgctcaag 9480 atggcttccc ctcggcagtt catcagggct aaatcaatct agccgacttg tccggtgaaa 9540 tgggctgcac tccaacagaa acaatcaaac aaacatacac agcgacttat tcacacgagc 9600 tcaaattaca acggtatata tcctgccagt cagcatcatc acaccaaaag ttaggcccga 9660 atagtttgaa attagaaagc tcgcaattga ggtctacagg ccaaattcgc tcttagccgt 9720 acaatattac tcaccggatc ctaaccggtt taattaaggc gcgccatgcc cgggcaagcg 9780 gccgcacaag tttgtacaaa aaagcaggct tctagaccta ggtggagtca tcacgcagac 9840 tatctcagca tgtgcgtagc acgcggccgc ctcccagtat cattatagtg aaagttttgg 9900 ctctctcgcc ggtggttttt tacctctatt taaaggggtt ttccacctaa aaattctggt 9960 atcattctca ctttacttgt tactttaatt tctcataatc tttggttgaa attatcacgc 10020 ttccgcacac gatatcccta caaatttatt atttgttaaa cattttcaaa ccgcataaaa 10080 ttttatgaag tcccgtctat ctttaatgta gtctaacatt ttcatattga aatatataat 10140 ttacttaatt ttagcgttgg tagaaagcat aatgatttat tcttattctt cttcatataa 10200 atgtttaata tacaatataa acaaattctt taccttaaga aggatttccc attttatatt 10260 ttaaaaatat atttatcaaa tatttttcaa ccacgtaaat ctcataataa taagttgttt 10320 caaaagtaat aaaatttaac tccataattt ttttattcga ctgatcttaa agcaacaccc 10380 agtgacacaa ctagccattt ttttctttga ataaaaaaat ccaattatca ttgtattttt 10440 tttatacaat gaaaatttca ccaaacaatg atttgtggta tttctgaagc aagtcatgtt 10500 atgcaaaatt ctataattcc catttgacac tacggaagta actgaagatc tgcttttaca 10560 tgcgagacac atcttctaaa gtaattttaa taatagttac tatattcaag atttcatata 10620 tcaaatactc aatattactt ctaaaaaatt aattagatat aattaaaata ttactttttt 10680 aattttaagt ttaattgttg aatttgtgac tattgattta ttattctact atgtttaaat 10740 tgttttatag atagtttaaa gtaaatataa gtaatgtagt agagtgttag agtgttaccc 10800 taaaccataa actataagat ttatggtgga ctaattttca tatatttctt attgctttta 10860 ccttttcttg gtatgtaagt ccgtaactgg aattactgtg ggttgccatg acactctgtg 10920 gtcttttggt tcatgcatgg atcttgcgca agaaaaagac aaagaacaaa gaaaaaagac 10980 aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa ttagtcactg gctgatcaag 11040 atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa cacgtgctta acatgcactt 11100 taaatggctc acccatctca acccacacac aaacacattg cctttttctt catcatcacc 11160 acaaccacct gtatatattc attctcttcc gccacctcaa tttcttcact tcaacacacg 11220 tcaacctgca tatgcgtgtc atcccatgcc caaatctcca tgcatgttcc aaccaccttc 11280 tctcttatat aatacctata aatacctcta atatcactca cttctttcat catccatcca 11340 tccagagtac tactactcta ctactataat accccaaccc aactcatatt caatactact 11400 ctaggatcca caaccaaaaa tggcagcgag gctgcaagag cagaaaggag gtgagatgga 11460 cacgaggatt gcgatcatag gcatgagcgc catacttcct tgtggaacca cggtgagaga 11520 gtcatgggag accataaggg ctggcattga ttgcctgtct gatcttcctg aggatcgtgt 11580 tgatgtgact gcgtactttg acccagtcaa gaccacaaag gacaaaatct actgcaagag 11640 aggaggtttc attcctgagt atgactttga tgcgcgtgag tttggtctca acatgttcca 11700 gatggaagat tctgatgcga atcagaccat ctcacttctc aaggtcaaag aagcacttca 11760 agatgctggg attgatgcac ttggaaagga gaagaaaaac ataggatgcg tgcttggcat 11820 tggaggtgga cagaagagtt cacatgagtt ctacagcaga ctcaactatg tggttgtgga 11880 gaaagtcttg agaaagatgg ggatgccaga ggaagatgtc aaagtggctg tggagaagta 11940 caaagcgaac ttcccagaat ggagacttga ctctttccct ggattccttg ggaatgtcac 12000 tgctggcaga tgcaccaaca cattcaactt ggatggcatg aactgcgttg tggatgctgc 12060 atgtgcaagc tccttgatag cagtcaaggt tgcgattgat gaactgctct atggtgactg 12120 cgacatgatg gtcactggtg caacctgcac agacaactcc attgggatgt acatggcttt 12180 ctcaaagaca cctgtgttca gcacggaccc aagtgtgaga gcgtatgatg agaaaacgaa 12240 agggatgctc attggtgagg gatcagccat gttggtgctc aaacgttatg cagatgcagt 12300 gagggatggt gatgagattc atgctgtgat tcgtggttgt gcaagctcaa gtgatggcaa 12360 agcagctggc atctacactc ccacaatcag tggtcaagag gaagccttga gacgtgcata 12420 caatcgtgcc tgcgttgacc ctgccactgt cactttggtt gagggtcatg ggactggcac 12480 tccagttgga gataggattg agttgactgc gttgaggaac ttgtttgaca aagcgtatgg 12540 tgaagggaac acagagaaag ttgcagttgg aagtatcaag tccagtattg gtcatttgaa 12600 ggctgttgct ggtcttgctg gaatgatcaa ggtcatcatg gctctcaaac acaagacttt 12660 gcctggaacg atcaatgtgg acaatccacc caatctctat gacaacactc ccatcaatga 12720 gtcatccctc tacatcaaca cgatgaacag accctggttc cctccacctg gtgttccgag 12780 acgtgctgga atatccagct ttggctttgg aggtgccaac taccatgcag tgttggaaga 12840 ggctgaacca gagcacacga cagcgtacag actcaacaaa cgtccacaac cggtgttgat 12900 gatggctgca actcctgctg cgttgcagag cctttgtgaa gctcagctga aagagtttga 12960 agctgcgatc aaggagaatg agacagtcaa gaatactgcc tacatcaagt gtgtcaagtt 13020 tggagaacag ttcaagttcc ctgggagcat acctgcgacc aatgcgagac tgggattctt 13080 ggtcaaagat gcggaggatg cgtgctccac gcttcgtgct atatgtgctc agtttgccaa 13140 agatgtgacc aaagaagcgt ggaggcttcc gagagaagga gtttctttcc gtgccaaagg 13200 cattgccacc aatggtgctg ttgctgcact cttctctgga caaggtgctc agtacacaca 13260 catgttcagt gaagttgcga tgaactggcc tcagttcaga cagtccattg ctgcaatgga 13320 cgctgcacag tccaaggttg ctggaagtga caaagacttt gaaagagtct ctcaagtcct 13380 gtacccaagg aaaccgtacg aacgtgagcc agaacaagat cacaagaaaa tctcactcac 13440 agcatacagc cagccgtcaa ccctggcatg tgctcttggt gcctttgaga ttttcaagga 13500 agctggattc actccagact ttgcagctgg acacagcttg ggtgagtttg ctgcgctcta 13560 tgcagctggt tgtgttgaca gagatgagtt gtttgaactt gtttgcagaa gggcaaggat 13620 catgggaggc aaagatgcac ctgccacacc caaaggttgc atggcagctg tcataggacc 13680 caacgcagag aacatcaagg ttcaagctgc caacgtctgg cttggaaact caaactctcc 13740 gtctcagact gtcattactg ggtctgttga agggattcaa gctgaaagtg ccagattgca 13800 gaaggaaggg ttcagagttg tgcctcttgc ttgtgagtct gccttccatt ctccacagat 13860 ggaaaatgcc agctcagctt tcaaagatgt catatccaag gtttccttcc gtacacccaa 13920 agcagaaaca aagctcttca gcaatgtttc tggagagacc tatccgacgg atgccagaga 13980 gatgctcact caacacatga cctcatctgt gaagttcctg acccaagtga ggaacatgca 14040 ccaagctggt gcgagaatct ttgttgagtt tggaccgaaa caagttctca gcaaacttgt 14100 ttctgaaacc ctcaaagatg acccgtctgt tgtcacagtg tctgtgaacc cagcttctgg 14160 cacggactct gacatacagt tgagggatgc tgcagttcag ctggttgtgg ctggtgtcaa 14220 tcttcaaggg tttgacaagt gggatgctcc tgatgccacg aggatgcaag ccatcaagaa 14280 aaagagaacc acactcagac tttcagctgc cacctatgtc agtgacaaga ccaagaaagt 14340 gagggatgct gccatgaacg acggaagatg tgtcacatac ctcaagggtg ctgcaccact 14400 catcaaggct cctgaacctg tggttgacga agctgccaag agggaggctg aaaggcttca 14460 gaaggagctt caagacgctc agaggcagct tgacgacgcc aaacgtgcag ctgcagaagc 14520 caactccaaa cttgctgctg ccaaggagga agccaagact gctgcagctt cagccaagcc 14580 tgcagttgac actgctgttg tggagaaaca tcgtgccatc ctcaagtcca tgctggctga 14640 gctggatggt tatggaagtg ttgatgcttc ttcccttcag caacagcaac agcaacagac 14700 tgctccggcg cctgttaaag ccgctgcccc tgccgctccc gttgcaagtg ctcctgctcc 14760 cgccgtgtcc aacgagttgc ttgagaaagc tgagacagtt gtgatggagg ttctcgctgc 14820 caagactgga tacgaaacag acatgattga ggcggacatg gagcttgaga ctgagttggg 14880 aattgacagc atcaagaggg ttgaaattct ttctgaagtc caagctatgc ttaacgttga 14940 ggcgaaagat gttgatgctc tctctaggac gagaacagtg ggtgaagttg tcaacgctat 15000 gaaggcagaa atcgctggtt cttccgctcc ggcgccggca gctgccgcac ctgcaccggc 15060 taaagcggct ccagctgcag ctgcaccagc tgtttctaat gagctgctcg aaaaggcaga 15120 aactgttgtg atggaagtcc ttgctgccaa aactggctac gaaactgaca tgatagaatc 15180 agacatggag cttgagacgg agcttggaat tgattccatc aagcgtgtgg aaatcctcag 15240 tgaagtccaa gccatgctca atgtggaagc taaggatgtg gatgctctct caaggaccag 15300 aactgtcggt gaggttgtca atgccatgaa ggctgagata gctggaggct ccgctcctgc 15360 gccggccgct gccgctcctg gtcccgctgc cgcggctcca gctccagctg cagccgctcc 15420 ggccgtctca aacgaactcc ttgaaaaagc tgagaccgtc gtgatggagg tcttggcagc 15480 taaaaccggc tatgagactg acatgattga gtctgacatg gaattggaga cagaacttgg 15540 gatagacagc atcaaacgtg ttgaaatcct cagtgaggtc caagctatgc tgaacgtgga 15600 agcgaaggat gttgatgctc tgtccagaac gaggaccgtt ggtgaggttg tggatgccat 15660 gaaggctgaa attgctggag ggtctgcgcc cgcgccagct gcagccgctc ctgcacccgc 15720 agccgcggca cctgctccag ctgcacctgc acctgccgtt tcttcagaac tcttggagaa 15780 ggctgagacg gttgtgatgg aggttttggc tgcaaagact ggctatgaga ccgacatgat 15840 tgaatctgat atggaattgg agacagagct tgggatcgat tccatcaaaa gagtggagat 15900 cctgagtgaa gtccaagcca tgctcaatgt tgaagctaag gatgttgatg cactttcaag 15960 aacaaggaca gtgggtgagg ttgtggatgc catgaaggcg gagattgctg gagggagcgc 16020 gcctgcgcca gctgccgcgg cacctgcacc ggcagccgca gctccagctc cagctgcacc 16080 agcaccggcc gcgcccgcgc cagctgtttc atccgaactc ctggaaaagg ccgagacggt 16140 tgtgatggag gtgctcgcag ctaagactgg ttacgagacc gacatgattg aaagtgacat 16200 ggagcttgaa accgagttgg gaatagatag catcaaacgt gttgaaatct tgtctgaggt 16260 ccaagctatg ttgaacgtgg aggcaaaaga tgtcgatgcg ctttcaagaa ccagaacagt 16320 cggtgaggtc gtggacgcca tgaaggctga aattgccgga agctctgcta gtgcgccagc 16380 tgcagccgca cctgcacctg ctgccgcggc acctgctcca gctgcagccg ctcctgccgt 16440 cagcaatgag ttgctggaga aagccgaaac agttgtgatg gaagtccttg ctgcaaaaac 16500 tggttacgaa acggacatga ttgagtcaga catggaattg gagaccgaac ttggaataga 16560 ttccatcaag agagtggaga tcctctctga ggtgcaagcc atgctcaacg ttgaggctaa 16620 agatgttgat gcactcagta ggacaagaac tgtgggggaa gttgtcgatg cgatgaaggc 16680 tgagattgct ggaggctctg ctcctgcgcc ggcagctgcc gcacctgcac ccgcagccgc 16740 ggcaccagct gtcagcaacg aattgcttga gaaggctgaa acagttgtca tggaagtgct 16800 ggctgcaaaa accggctatg agactgacat gattgagtct gacatggaac ttgagacgga 16860 attgggcatt gacagcatca agcgtgttga gatcctcagt gaagtccaag caatgctcaa 16920 tgtggaggct aaggatgtgg atgccctctc aaggaccaga acagttggtg aggtcgttga 16980 tgcgatgaag gcggagatcg ctggaagttc cgcgcctgcg ccagctgcag ccgctcctgc 17040 acccgcagcc gcggcaccgg ctccagctgc agccgcaccg gccgtctcct cagaactcct 17100 ggaaaaggct gagacagttg tgatggaagt ccttgctgcg aaaactggct atgagaccga 17160 catgattgaa tctgacatgg aactggagac cgagcttgga attgactcca tcaaacgtgt 17220 tgaaatcctc tctgaggttc aagcgatgtt gaatgtggag gccaaagatg ttgatgctct 17280 ttccagaaca aggacggtgg gagaggtggt tgatgccatg aaggctgaga tagctggggg 17340 ttctgctcct gcgccagctg cagccgctcc tgctcccgct gccgcggcac cagctgtcag 17400 caacgaactc cttgaaaaag ctgaaactgt tgtgatggag gttttggctg caaagactgg 17460 atacgagact gacatgatag agtcagacat ggagttggaa acagagcttg ggattgatag 17520 catcaagcgt gtggaaatcc tttctgaggt tcaagccatg ctgaacgttg aggccaaaga 17580 tgtcgatgct ttgtcaagga ccagaacggt tggagaagtg gtcgatgcca tgaaggctga 17640 gatagctggt ggatcagccc ctgctccggc tgcagccgct cctgcaagtg ccggtgctgc 17700 accagctgtg aagattgatt cagttcatgg tgctgactgt gacgatttgt cactgatgca 17760 cgcaaaggtg gttgacatca gaaggccaga tgaactgatt ctggaaagac cggagaaccg 17820 tccagttctt gtcgttgacg atggctcaga gttgacactt gctcttgtga gggtgttggg 17880 agcctgtgca gttgtcttga cctttgaggg attgcagttg gctcagagag ctggtgcagc 17940 tgccattcgt catgttctgg ctaaggacct ttcagcagaa tcagctgaga aggccatcaa 18000 agaggctgag caaagatttg gagcgttggg aggcttcata agccagcaag cagaaaggtt 18060 tgaaccagct gagatactgg gtttcacact catgtgtgcg aagtttgcca aagcctctct 18120 ctgcactgct gtggctggag gtcgtcctgc cttcatcggt gtggctaggc ttgacggcag 18180 acttggattc acctctcaag gcacttctga cgcactcaag agagcacagc gtggagcgat 18240 ctttggactc tgcaagacaa tagggttgga gtggtcagaa tctgatgtgt tcagccgtgg 18300 tgtggacatt gctcaaggga tgcacccaga agatgctgca gttgccatag ttagggagat 18360 ggcgtgtgct gacatcagaa tcagagaggt tgggattgga gcaaaccagc aaagatgcac 18420 aatcagagct gccaagttgg agactgggaa ccctcagaga cagatagcga aagacgatgt 18480 gcttctggtt tctggaggtg ctcgtgggat cacacctctt tgcatccgtg agatcacgcg 18540 tcagattgct ggaggcaagt acattctgtt gggtcgtagc aaagtttctg cgtctgaacc 18600 agcatggtgt gctggcatca ctgatgagaa ggctgtgcag aaagctgcca cccaagagtt 18660 gaagagggcc ttcagtgctg gtgaaggacc caaacccact ccaagggctg tcacgaaact 18720 tgtgggatct gttcttggtg ctcgtgaagt gagaagctcc attgctgcca ttgaagctct 18780 gggtggcaaa gcgatctaca gctcttgtga tgtgaactct gctgcagatg ttgccaaagc 18840 agttagggat gcagagtccc agcttggtgc cagagttagt ggaatcgttc acgcttctgg 18900 tgtgctccgt gatcgtctca tagagaagaa actccctgac gagtttgatg ctgtctttgg 18960 gaccaaagtc actgggttgg agaatcttct ggctgcagtt gatcgtgcca acctcaaaca 19020 catggttctc ttcagctccc ttgctggttt ccacggcaat gttggtcagt ctgactatgc 19080 gatggcaaac gaagctctca acaagatggg tttggaactt gcgaaggatg tcagtgtcaa 19140 gtccatctgc tttggtccct gggatggtgg gatggtgaca cctcaactca agaaacagtt 19200 tcaagaaatg ggagttcaga tcattcctcg tgaaggaggt gctgatacag ttgcgaggat 19260 tgtgttggga tcaagtcctg ctgagatcct tgttggaaac tggaggacac catccaagaa 19320 agttggaagt gacacgatca ccctgcatag aaagatcagt gccaagtcca atcccttctt 19380 ggaggatcat gtcatacaag gcagaagggt gttgccaatg acccttgcta ttgggagcct 19440 tgctgagacc tgccttggac tcttccctgg ctactctctc tgggccatag atgacgcaca 19500 gctcttcaaa ggtgtcactg ttgatggaga cgtcaactgt gaagtgaccc tcacaccgtc 19560 cactgctccg agtgggaggg tgaatgtcca agcgacgctc aagacgttct caagtggaaa 19620 acttgttcca gcctacagag ctgtcatagt gttgtccaac caaggtgctc cacctgccaa 19680 tgcaaccatg cagcctccga gccttgatgc agatcctgct ttgcaagggt cagtctatga 19740 tggcaagact ctctttcatg gtcctgcgtt cagaggcatt gatgacgttc tcagctgcac 19800 caagagccag cttgttgcca agtgttctgc tgttcctgga tcagatgcag ccagaggaga 19860 gtttgcaact gacactgatg cacatgatcc ctttgtcaat gatcttgcct ttcaagcaat 19920 gttggtctgg gtgaggagaa cgctgggaca agctgcactc ccgaactcca ttcagaggat 19980 agtccaacac agaccggtcc cacaagacaa acccttctac atcaccctca gatccaatca 20040 gagtggtgga catagccagc acaaacatgc acttcagttc cacaacgaac aaggtgatct 20100 cttcattgat gttcaagcct ctgtcattgc gacagactca cttgcgttct gagtagttag 20160 cttaatcact taggtcacca gtatgaacta aaatgcatgt aggtgtaaga gctcatggag 20220 agcatggaat attgtatccg accatgtaac agtataataa ctgagctcca tctcacttct 20280 tctatgaata aacaaaggat gttatgatat attaacactc tatctatgca ccttattgtt 20340 ctatgataaa tttcctctta ttattataaa tcatctgaat cgtgacggct tatggaatgc 20400 ttcaaatagt acaaaaacaa atgtgtacta taagactttc taaacaattc taactttagc 20460 attgtgaacg agacataagt gttaagaaga cataacaatt ataatggaag aagtttgtct 20520 ccatttatat attatatatt acccacttat gtattatatt aggatgttaa ggagacataa 20580 caattataaa gagagaagtt tgtatccatt tatatattat atactaccca tttatatatt 20640 atacttatcc acttatttaa tgtctttata aggtttgatc catgatattt ctaatatttt 20700 agttgatatg tatatgaaaa ggtactattt gaactctctt actctgtata aaggttggat 20760 catccttaaa gtgggtctat ttaattttat tgcttcttac agataaaaaa aaaattatga 20820 gttggtttga taaaatattg aaggatttaa aataataata aataataaat aacatataat 20880 atatgtatat aaatttatta taatataaca tttatctata aaaaagtaaa tattgtcata 20940 aatctataca atcgtttagc cttgctggaa cgaatctcaa ttatttaaac gagagtaaac 21000 atatttgact ttttggttat ttaacaaatt attatttaac actatatgaa attttttttt 21060 tttatcagca aagaataaaa ttaaattaag aaggacaatg gtgtcccaat ccttatacaa 21120 ccaacttcca caagaaagtc aagtcagaga caacaaaaaa acaagcaaag gaaatttttt 21180 aatttgagtt gtcttgtttg ctgcataatt tatgcagtaa aacactacac ataacccttt 21240 tagcagtaga gcaatggttg accgtgtgct tagcttcttt tattttattt ttttatcagc 21300 aaagaataaa taaaataaaa tgagacactt cagggatgtt tcaaccctta tacaaaaccc 21360 caaaaacaag tttcctagca ccctaccaac gaattcgcgg ccgcttaatt aagatgagtg 21420 atactcagga ctcaggactc actctgctga tcactagtgc tagcctcgag gtcgacacaa 21480 ctttgtatac aaaagttgtc tagacctagg ctcagcaact catccagtca agttcgagtc 21540 agtgagttgg gcggccgcag cttatagtaa agattttttt gttaataaaa tgaaagttaa 21600 tatttatgga tttatttgaa catatttaca agtttttgtt aataaattta tttacagaag 21660 tgttaaattt gaaaatgtgt taaatgagag ttaatattta tggatataat attactttat 21720 ttaaaaataa aatgaattta tattaaaata tgatttggta aaagatatta taaagaggaa 21780 cttggatgag aaagacgacg atgggcaaaa gttgcatggc acaccgcttt tgttcagtaa 21840 catctgcact gtggcgcctt tttcacactc accatgcatg ctgccacgtc agctttcaca 21900 ctatgacacc actggccata cacgttgcca cctcagcttt ctcctcttct cactatgaca 21960 cgactggcca tgcatgcatg catgctgcca cctcagctcc cgcctcttca cccgtgtctt 22020 tctctcatgt cgcagtgctg cgtgccaacc tcttctctct ataaatagag agaagagaat 22080 gatggttaaa ctcaaaccaa aacaagaaaa catacacaaa tagcaaaacg gatccacaac 22140 caaaaatggc tgcaaggaac gtgagtgcgg ctcatgagat gcatgacgag aagaggatag 22200 ctgttgtggg catggctgtt cagtatgctg gatgcaaaac gaaagacgag ttctgggaag 22260 ttctcatgaa tggaaaagtt gagagcaaag tgatcagcga caaacgtctt ggttcaaatt 22320 accgtgctga acactacaaa gctgaaagga gcaagtatgc tgacaccttc tgcaatgaga 22380 catatggaac tcttgatgag aacgaaatag acaatgagca tgaactcctt ttgaaccttg 22440 ccaaacaagc cttggctgaa acatctgtca aagattcaac aagatgtggc atagtttctg 22500 gatgcctcag cttcccgatg gacaacctcc aaggtgaact cttgaatgtg taccagaacc 22560 atgttgagaa gaaacttggt gccagagttt tcaaagatgc ctctcactgg tcagaaagag 22620 aacagtccaa caaaccagag gctggtgaca gaagaatctt catggaccct gcttcctttg 22680 ttgctgagga actcaatctt ggcgctctcc actactctgt tgatgctgcc tgtgccactg 22740 cgctctatgt gctcagactg gcgcaagatc acttggtctc tggtgcagct gatgtgatgc 22800 tttgtggtgc aacatgcctg ccggagccgt ttttcatact ctctgggttc tcaaccttcc 22860 aagccatgcc tgttggaacg ggtcagaatg tgtccatgcc gctgcacaaa gattctcaag 22920 gattgacccc tggtgaagga ggttccatca tggtcctcaa acgtttggat gacgcaatca 22980 gagatggaga tcacatctat ggaacacttt tgggagccaa tgtcagcaac agtggcactg 23040 gtctgccact taaacccctt ttgccgtcag agaaaaagtg cctcatggac acctacacac 23100 gtatcaatgt gcatccccac aagattcagt atgttgaatg ccatgcaact gggactccac 23160 aaggtgatcg tgtggaaatt gatgctgtga aggcttgctt tgaaggcaaa gtcccacgtt 23220 ttggaaccac aaaagggaac tttggacaca cattggtggc agctgggttt gctggcatgt 23280 gcaaagtgct tctctccatg aagcacggga tcattccacc cactccggga atagatgacg 23340 aaacgaagat ggaccctctg gttgtctctg gtgaggccat tccctggcca gaaacgaatg 23400 gagaaccgaa gcgtgctggt ctctcagcgt ttggatttgg tgggaccaat gctcatgctg 23460 tctttgagga acatgaccca agcaatgctg catgcactgg ccatgactcc atttctgctc 23520 tctctgcacg ttgtggtggt gagtcaaaca tgagaatagc aatcactggg atggatgcca 23580 cttttggtgc tttgaaagga ttggatgctt ttgaaagggc catctacact ggtgctcatg 23640 gtgctatacc actcccagaa aagagatgga gattcctggg gaaagacaag gacttccttg 23700 atctttgtgg agtcaaagca acaccgcatg gctgttacat tgaagatgtt gaagttgatt 23760 tccagaggct gaggactcca atgactcctg aagatatgct cttgcctcaa cagcttctgg 23820 ctgtcaccac aattgatagg gccatattgg actctggcat gaagaaaggt ggaaatgtgg 23880 ctgtctttgt ggggttgggg accgatcttg aactctaccg tcaccgtgca agggttgctc 23940 tcaaggagag agtgaggcct gaagccagca agaaactcaa tgacatgatg cagtacatca 24000 atgattgtgg gacaagcact tcttacacca gctacatagg caacttggtt gcaacaaggg 24060 tcagttcaca atggggcttc acgggacctt ccttcacaat cacagaaggc aacaattctg 24120 tgtacagatg tgctgaactt ggcaaatacc tcttggaaac gggagaggtt gatggggttg 24180 tggttgcggg tgttgatctc tgtgggagtg ctgagaacct ttacgtcaag tcaaggcgtt 24240 tcaaggtgtc tacatctgac accccgagag ccagctttga tgcagctgct gatggttact 24300 ttgttggaga gggctgtggt gcatttgtgc tcaagcgtga gacaagctgc accaaggatg 24360 acagaatcta tgcttgcatg gatgccatag ttcctggaaa tgttccatct gcatgtttga 24420 gagaggcatt ggatcaagcc agagtcaagc ctggagacat cgagatgttg gagctttctg 24480 ctgattcagc gaggcatttg aaggacccgt ctgttctccc aaaggaactc actgctgagg 24540 aagagattgg cggacttcag acgattcttc gtgacgatga caagctccca aggaatgttg 24600 caactggaag tgtcaaggcc actgttggtg acactggtta tgcttctggt gctgccagtt 24660 tgattaaggc tgcgctttgc atctacaacc gttacctgcc cagcaatgga gatgactggg 24720 atgaacctgc acctgaagca ccctgggaca gcacactctt tgcgtgccag acctcaagag 24780 cttggttgaa gaaccctggt gagagacgtt atgcggctgt ttccggagtc agtgaaacca 24840 gaagttgcta cagtgtgctg ctttctgaag cagagggaca ctacgagaga gagaacagaa 24900 tcagtttgga tgaggaagca cccaagctca ttgtgttgag agcagactcc catgaagaga 24960 ttcttggaag attggacaag ataagggaaa ggttcttgca gcccacgggt gctgcaccca 25020 gagagtctga attgaaggcg caagcccgta ggatctttct tgaactcctt ggtgagaccc 25080 ttgcccaaga tgcagcttca tctggaagtc agaagcccct tgctctcagc ttggtctcca 25140 ctccatccaa gctccagaga gaggtggaac ttgcggcaaa aggcattccg agatgtctca 25200 agatgagacg tgattggagt tcccctgctg gaagcagata tgcgcctgag cctttggcca 25260 gtgacagagt tgccttcatg tatggagaag gaaggagccc ttactatggc atcacacaag 25320 acattcatag gatttggcct gaacttcatg aggtcatcaa tgagaaaaca aaccgtctct 25380 gggctgaagg tgacagatgg gtcatgccaa gggccagctt caagagtgag cttgagtcac 25440 aacagcaaga gtttgacaga aacatgattg aaatgttccg tcttggaatc ctcacctcaa 25500 ttgccttcac aaacctggcg agagatgttc tcaacatcac gcccaaagca gcctttggac 25560 tcagtttggg agagatttcc atgatctttg ctttctccaa gaaaaacggt ttgataagcg 25620 atcagttgac aaaggatttg agagagtcag atgtctggaa caaagctctg gctgttgagt 25680 tcaatgcgct cagagaagcc tggggcattc cacagtctgt cccaaaagat gagttctggc 25740 aaggttacat agtgagggga acgaaacaag acatagaagc agccatagca ccagactcaa 25800 agtatgtgag gttgaccatc atcaatgatg ccaacactgc tctcatcagt ggaaagcctg 25860 atgcatgcaa agctgcgatt gctcgtcttg gtggcaacat ccctgcgctt ccagtcactc 25920 aaggcatgtg tgggcattgc ccagaggtgg gaccttacac caaggacatc gcaaagatcc 25980 acgccaacct tgagttccct gtggttgatg gtcttgatct ctggactacc ataaaccaga 26040 agaggcttgt gccaagagcc acgggtgcca aagatgaatg ggctccttca tcctttgggg 26100 agtatgctgg acagctctat gagaaacaag ccaacttccc tcagatagtt gaaaccatct 26160 acaagcagaa ctatgatgtg tttgttgaag ttgggccaaa caatcacaga agcacagcag 26220 tgagaaccac gcttggacca cagagaaacc accttgctgg tgccatagac aagcagaacg 26280 aggatgcttg gacgacaata gtcaagctcg tggcttcatt gaaggctcac cttgttcctg 26340 gagtgaccat cagtccgctc taccactcca agctcgttgc agaggctgag gcttgttatg 26400 ctgcgctctg taaaggtgag aaacccaaga aaaacaagtt tgtgagaaag attcagttga 26460 atggcagatt caacagcaag gcagacccca tctcctcagc agaccttgca tccttcccac 26520 ctgcggaccc tgcgattgaa gctgccatct cttcaaggat catgaagcct gtggctccga 26580 aattctatgc gcgtctcaac attgatgagc aagatgagac cagagatccg atcttgaaca 26640 aggacaatgc gccttccagc tccagctctt ccagcagttc ctcttccagc agttcatctc 26700 cgtctcctgc tccttcagct ccagttcaga agaaagctgc cccagctgcg gagacaaaag 26760 ctgttgccag tgctgatgca cttcgtagtg ccctgcttga tcttgactcc atgcttgcgc 26820 tcagctctgc aagtgcttct ggcaaccttg ttgagactgc gccatctgat gccagtgtca 26880 ttgtccctcc gtgcaacatt gctgatcttg gcagcagagc tttcatgaaa acttatggtg 26940 tttctgcgcc tctgtacacc ggtgcaatgg ccaagggaat tgcctctgcg gacttggtga 27000 tcgcagctgg aaggcaagga atccttgcgt catttggtgc tggaggcctt ccaatgcaag 27060 ttgtgcgtga gtccattgaa aagattcaag ctgccctgcc caatggacca tatgctgtca 27120 acctcatcca ttctcctttt gacagcaacc ttgagaaggg aaatgttgat ctcttcttgg 27180 aaaagggtgt cacttttgtt gaggcttcag ccttcatgac gttgacaccg caagttgtca 27240 gatacagagc ggctgggttg acgaggaatg cagatgggtc agtcaacata aggaacagaa 27300 tcattgggaa agtctccaga actgagctgg ctgaaatgtt catgcgtcct gcacctgagc 27360 atcttttgca gaagctcatt gcttctgggg agatcaatca agaacaagct gagttggcca 27420 gacgtgttcc tgtggctgat gacattgcgg ttgaagctga ttctggtgga cacactgaca 27480 accgtccaat ccatgtcatt ctgcctctga ttatcaacct gagggatagg cttcacagag 27540 agtgtggata cccagcaaac ctgagggttc gtgttggtgc tgggggtggc attgggtgtc 27600 cccaagctgc gctggcaact ttcaacatgg gtgcctcttt cattgtcact ggaacagtga 27660 accaagttgc caaacaatct gggacgtgtg acaatgtgag gaaacagctt gcgaaagcaa 27720 cttactcaga tgtttgcatg gcaccggcag ctgacatgtt tgaggaaggt gtgaaacttc 27780 aagttttgaa gaaaggaaca atgttccctt caagagccaa caaactctat gagttgttct 27840 gcaagtatga ttcttttgag tccatgcctc cagcagagct tgcgagggtt gagaaaagaa 27900 tcttcagtcg tgcattggaa gaggtctggg atgagaccaa gaacttctac atcaaccgtc 27960 tccacaaccc agagaagata cagagggctg agagggaccc caaactgaag atgtcactgt 28020 gtttcagatg gtatctgtcc ctggcttcaa ggtgggccaa cactggagct tctgacagag 28080 tcatggacta ccaagtttgg tgtggtcctg ccattggttc attcaatgat ttcatcaaag 28140 gaacttacct tgatccggca gtggcaaatg agtacccgtg tgttgtgcag atcaacaaac 28200 agattcttag aggagcgtgc ttcttgaggc gtcttgaaat actgagaaat gcaaggcttt 28260 cagatggtgc agctgcactt gtggcgagca ttgatgacac atatgtccct gctgagaagc 28320 tgtgagtagt tagcttaatc acttaggtca cccagtccaa gcccggccta gcacgatgat 28380 catgagtgtt gctattgatg tatgttaata ctatacacag tcatcgtgtg tgttttataa 28440 aactgtaagc ttaggatgtt tgaggctaat gtaattagca ctacaccgta ataaatgaga 28500 ggtttacttt atgttttaat aatgttctta cccttgctgc tctctaaact gtaatggcgt 28560 acggttagct aaactaaagt aaaaatcgat tcgcttgagt aaaattagtg accgggaaag 28620 atcatcgact actactgctc agagatgtaa tcggtaatta agacttcaag ctcaataatt 28680 aattaggagt aaatctggct tcgaattttt aaattgtcaa cttccaaaga aggattggca 28740 agactcaatc tcaattgccc aacaaacata tggctcttta caaatttgga atctcttttt 28800 gatggcaaga acgtcgtaat tcctctgtcg atctcatgta attaccaaac tcatctactt 28860 attactttct ttatatgcac ttactgatgc ggccgcttaa ttaatctgac ctgaccacta 28920 gacctgagtg agtacagatg tgctactagt gctagcctcg aggtcgacca acttttctat 28980 acaaagttgt ctagacctag gactaggcac tgagtgactc actgacctga ctgagtgact 29040 ggcggccgca gcttatagta aagatttttt tgttaataaa atgaaagtta atatttatgg 29100 atttatttga acatatttac aagtttttgt taataaattt atttacagaa gtgttaaatt 29160 tgaaaatgtg ttaaatgaga gttaatattt atggatataa tattacttta tttaaaaata 29220 aaatgaattt atattaaaat atgatttggt aaaagatatt ataaagagga acttggatga 29280 gaaagacgac gatgggcaaa agttgcatgg cacaccgctt ttgttcagta acatctgcac 29340 tgtggcgcct ttttcacact caccatgcat gctgccacgt cagctttcac actatgacac 29400 cactggccat acacgttgcc acctcagctt tctcctcttc tcactatgac acgactggcc 29460 atgcatgcat gcatgctgcc acctcagctc ccgcctcttc acccgtgtct ttctctcatg 29520 tcgcagtgct gcgtgccaac ctcttctctc tataaataga gagaagagaa tgatggttaa 29580 actcaaacca aaacaagaaa acatacacaa atagcaaaac ggatccacaa ccaaaaatgg 29640 cgcttcgtgt caagaccaac aagaagccat gttgggagat gaccaaggag gaactgacct 29700 ctggcaaaac tgaggtgttc aactatgagg aactcctgga gtttgcagag ggtgacatag 29760 ctaaggtttt tggtccggag tttgctgtca tagacaagta ccccagaagg gtgaggctgc 29820 ctgctcgtga atacctgctt gtgacaaggg tcacactcat ggatgccgag gtcaacaact 29880 acagagttgg tgccagaatg gtcactgagt atgatcttcc agtgaatggc gaattgtctg 29940 agggaggtga ttgtccctgg gcagttctgg ttgagagtgg acagtgcgat ttgatgctga 30000 tatcatacat gggaattgat ttccagaacc aaggtgacag agtttaccgt ctgctcaaca 30060 ccacgttgac attctatggg gttgcccatg agggagagac ccttgagtat gacatcagag 30120 tgaccggttt tgccaaacgt ttggatggtg gcatctccat gttcttcttt gagtatgact 30180 gctatgtcaa tgggaggctt ctcattgaga tgagagatgg ctgtgctggc ttcttcacaa 30240 atgaggagct tgatgcggga aagggtgtgg tgttcacaag aggggatttg gctgccagag 30300 ctaagattcc aaagcaagat gtttctccct atgctgtcgc tccctgcctt cacaaaacaa 30360 aactcaacga aaaggagatg cagaccttgg ttgacaaaga ttgggcttcc gtttttggct 30420 caaagaatgg catgccggag atcaactaca aactctgcgc acgtaagatg ctcatgattg 30480 atagagtcac cagcattgac cataagggag gtgtgtacgg tttgggtcag ttggttggtg 30540 agaaaatctt ggagagggac cattggtact tcccatgcca ttttgttaag gatcaagtca 30600 tggctggatc tcttgtcagc gacggttgtt cacaaatgct caagatgtac atgatctggc 30660 tcggtttgca ccttaccacc ggaccatttg acttcagacc cgtcaacgga cacccgaaca 30720 aagttaggtg taggggtcag atatctcctc ataagggaaa gttggtctac gtcatggaga 30780 tcaaagagat gggattcgac gaggataacg atccgtacgc cattgctgac gtcaacatca 30840 ttgacgttga tttcgaaaag ggtcaagatt tcagccttga tagaatcagc gactacggaa 30900 agggggatct taacaaaaag atcgtcgtgg acttcaaggg cattgctctc aagatgcaga 30960 agagatccac gaacaagaac ccctccaaag ttcagcccgt ttttgctaac ggtgcagcca 31020 ctgtgggacc ggaagcgtcc aaggccagct ctggtgccag tgctagtgct tctgctgccc 31080 ctgcgaagcc tgctttctca gcagatgttc ttgcgccaaa gccagttgca cttcctgaac 31140 atatcctcaa gggtgacgct ctcgctccga aagaaatgtc ctggcatccc atgtcaaaac 31200 ttcctggcaa cccgacgccg ttcttctccc ctagttctta ccctccgagg gcaatttgct 31260 tcatcccttt ccctggcaac ccacttgaca acaattgcaa agctggagaa atgcctctga 31320 actggtacaa catgtcagag ttcatgtgtg gaaaagttag caactgcttg ggaccagagt 31380 tcgcaagatt tgataagtca aatacgagca gaagccctgc ttttgatttg gctcttgtga 31440 ctagagttgt tgaagtcaca aacatggaac atggcaagtt cctgaatgtt gattgcaatc 31500 ccagcaaagg cacaatggtg ggggagtttg actgtcctca agatgcgtgg ttctttgatg 31560 gttcttgcaa tgatgggcac atgccttatt ccatcatcat ggaaatcgga cttcagacat 31620 ccggtgttct caccagtgtg ttgaaagcac cactgacgat ggacaaagat gacattctct 31680 ttaggaacct tgatgcaagt gctgaaatgg tgaggccaga tgtggatgtg agggggaaaa 31740 ccataaggaa tgtgaccaag tgcactggat atgcaatgtt gggaaagatg gggattcaca 31800 gattcacgtt tgagttgtct gttgatggcg tggttttcta caaaggaagc acttcctttg 31860 gatggttcac tccagaggtg tttgctcagc aagctggact tgacaatggg aaaaagacgg 31920 agccttggtg caaaactaac aacacgagtg tgagaagagt tgaaatcgca tcagccaaag 31980 gcaaagaaca gctgactgag aaacttccag atgcaactaa tgctcaagtt ctcagaaggt 32040 cagaacagtg tgagtatttg gattacctca acattgctcc tgattctggg ctgcatggga 32100 aaggatatgc gcatggacac aaagatgtta acccacaaga ctggttcttc tcttgtcact 32160 tttggtttga tcctgtgatg cctggatctt tgggaattga gtcaatgttc cagctcattg 32220 aggcatttgc ggtggaccag aacataccgg gagaatacaa tgtgagcaat ccgacctttg 32280 cccatgcccc tggaaaaacg gcgtggaagt accgtggtca attgacacca aagaaccgtg 32340 cgatggactg tgaggtgcac atagtttcaa tcacagcctc tcctgaaaat ggaggctacg 32400 ttgacatagt ggcagatgga gcactttggg ttgatggtct ccgtgtgtat gaagccaaag 32460 aactcagagt tcgtgttgtc agtgcaaaac ctcaagccat tccggatgtt caacaacagc 32520 cacccagcgc aaaagcggac cctgggaaaa ctggagttgc actcagtccg actcagttga 32580 gagatgtcct gcttgaagtg gacaatccat tgtatcttgg tgttgaaaac tccaatttgg 32640 tgcagtttga atccaaacct gcaacttctt caaggattgt cagtatcaaa ccatgctcca 32700 taagtgatct tggtgacaag tccttcatgg aaacgtacaa tgtgtcagca cctctgtaca 32760 ctggagcaat ggccaaggga attgcgtctg cggaccttgt cattgctgct gggaagagga 32820 agatattggg ttcttttgga gcgggtgggc ttccaatgca ccatgtgagg gcagctttgg 32880 agaagataca agcggctctg cctcaaggac catatgctgt gaacctcatt cattcacctt 32940 ttgacagcaa cttggaaaag gggaatgttg atctcttcct cgaaaagggc gttactgtgg 33000 tggaggcttc tgcattcatg accctcactc cccaagttgt tcgttaccgt gcagccggac 33060 tctcaaggaa cgctgatggg agtgtcaaca tcagaaaccg tatcattgga aaagtgtcca 33120 gaacagaact cgccgaaatg ttcatcagac cggctccgga acacctcctt gagaaactca 33180 tagcttctgg tgagataaca caagaacaag ccgaacttgc ccgtcgtgtt cctgtcgcag 33240 acgacatcgc tgttgaagct gattctggtg gtcacactga caaccgtccc atccacgtca 33300 tcttgccttt gatcatcaac cttaggaaca gactgcacag agagtgtggt tatcctgcgc 33360 accttagagt cagagttgga gctggaggcg gtgttggctg cccacaagct gctgcggctg 33420 ccctcacaat gggagctgcc ttcatagtga ctgggactgt caatcaagtt gctaaacagt 33480 ccgggacatg tgacaacgtg agaaaacaac tcagtcaagc cacctacagt gacatttgca 33540 tggctccagc agctgatatg tttgaggaag gtgtgaaact ccaagttctc aagaagggaa 33600 caatgttccc atcaagagct aacaaattgt acgaactctt ttgcaagtac gactctttcg 33660 attcaatgcc tcctgctgaa cttgaaagga tcgagaagag gatcttcaag agggcacttc 33720 aagaggtctg ggaagagacc aaggactttt acatcaatgg tttgaagaat ccagagaaga 33780 tacagagagc ggaacatgac cccaaactca agatgtcttt gtgtttcaga tggtatcttg 33840 gtcttgcatc acgttgggcc aacatgggtg ctcctgatcg tgtcatggac tatcaagtct 33900 ggtgtggtcc agccattgga gccttcaatg acttcatcaa aggaacatat cttgatcctg 33960 ctgtctctaa tgagtaccca tgtgttgtcc agatcaacct tcagattttg cgtggtgcat 34020 gttacctgag gcgtcttaat gctctgagaa atgatccgag gattgatctt gagactgaag 34080 atgctgcctt tgtctatgag ccaacgaatg cgctttgagt agttagctta atcacttagg 34140 tcacccagtc caagcccggc ctagcacgat gatcatgagt gttgctattg atgtatgtta 34200 atactataca cagtcatcgt gtgtgtttta taaaactgta agcttaggat gtttgaggct 34260 aatgtaatta gcactacacc gtaataaatg agaggtttac tttatgtttt aataatgttc 34320 ttacccttgc tgctctctaa actgtaatgg cgtacggtta gctaaactaa agtaaaaatc 34380 gattcgcttg agtaaaatta gtgaccggga aagatcatcg actactactg ctcagagatg 34440 taatcggtaa ttaagacttc aagctcaata attaattagg agtaaatctg gcttcgaatt 34500 tttaaattgt caacttccaa agaaggattg gcaagactca atctcaattg cccaacaaac 34560 atatggctct ttacaaattt ggaatctctt tttgatggca agaacgtcgt aattcctctg 34620 tcgatctcat gtaattacca aactcatcta cttattactt tctttatatg cacttactga 34680 tgcggccgct taattaatca cgagtggatg actgtcacga ctgactcatg actgactact 34740 agtgctagcc tcgaggtcga cacaactttg tataataaag ttgtctagac ctaggagctg 34800 tgctgggtaa ctcacatgag tcactggtca catcagcggc cgcagcttat agtaaagatt 34860 tttttgttaa taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt 34920 ttgttaataa atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat 34980 atttatggat ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt 35040 tggtaaaaga tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc 35100 atggcacacc gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat 35160 gcatgctgcc acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca 35220 gctttctcct cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca 35280 gctcccgcct cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc 35340 tctctataaa tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac 35400 acaaatagca aaacggatcc acaaccaaaa atgcttcagc acacttggct tccgaagcct 35460 cccaatctga ccctcttgtc agatgaggtt catctctgga ggattcctct tgaccagcct 35520 gagtcacaac ttcaagacct tgctgccacc ctgagcagtg atgaattggc gagggcaaac 35580 agattctact ttccagaaca cagaaggcgt ttcactgctg ggagaggcat cctcagatcc 35640 atcttgggtg gatacttggg agtggaaccg ggtcaagtca agtttgatta tgagtcccgt 35700 gggaaaccga tccttggtga cagatttgct gagagtggac tcctgttcaa cttgtctcac 35760 agccagaacc ttgccttgtg tgctgtcaac tacacgcgtc aaataggcat tgatcttgaa 35820 tatctgcgtc caacatctga cttggagtct cttgcaaaga ggttctttct cccaagagaa 35880 tatgaactct tgaggtcact ccctgatgag cagaaacaga agattttctt tcgttactgg 35940 acttgcaaag aggcttatct caaagcaacg ggagatggaa tagccaaact tgaagagatc 36000 gagatagcac tcaccccaac agaacctgcc aagctccaaa cagctcctgc gtggtctctg 36060 ttggagcttg tgccagatga caattgtgtt gcagctgtgg ctgttgcggg ttttggttgg 36120 cagcccaagt tctggcatta ctgagtagtt agcttaatca cttaggtcac ccagtccaag 36180 cccggcctag cacgatgatc atgagtgttg ctattgatgt atgttaatac tatacacagt 36240 catcgtgtgt gttttataaa actgtaagct taggatgttt gaggctaatg taattagcac 36300 tacaccgtaa taaatgagag gtttacttta tgttttaata atgttcttac ccttgctgct 36360 ctctaaactg taatggcgta cggttagcta aactaaagta aaaatcgatt cgcttgagta 36420 aaattagtga ccgggaaaga tcatcgacta ctactgctca gagatgtaat cggtaattaa 36480 gacttcaagc tcaataatta attaggagta aatctggctt cgaattttta aattgtcaac 36540 ttccaaagaa ggattggcaa gactcaatct caattgccca acaaacatat ggctctttac 36600 aaatttggaa tctctttttg atggcaagaa cgtcgtaatt cctctgtcga tctcatgtaa 36660 ttaccaaact catctactta ttactttctt tatatgcact tactgatgcg gccgcttaat 36720 taactactgt cactgaggcc gtagacgagt acggactgat ctaactagtg ctagcctcga 36780 ggtcgacacc cagcttt 36797 <210> 54 <211> 42674 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9344 <400> 54 gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat ctcagcatgt 60 gcgtagcacg cggccgcctc ccagtatcat tatagtgaaa gttttggctc tctcgccggt 120 ggttttttac ctctatttaa aggggttttc cacctaaaaa ttctggtatc attctcactt 180 tacttgttac tttaatttct cataatcttt ggttgaaatt atcacgcttc cgcacacgat 240 atccctacaa atttattatt tgttaaacat tttcaaaccg cataaaattt tatgaagtcc 300 cgtctatctt taatgtagtc taacattttc atattgaaat atataattta cttaatttta 360 gcgttggtag aaagcataat gatttattct tattcttctt catataaatg tttaatatac 420 aatataaaca aattctttac cttaagaagg atttcccatt ttatatttta aaaatatatt 480 tatcaaatat ttttcaacca cgtaaatctc ataataataa gttgtttcaa aagtaataaa 540 atttaactcc ataatttttt tattcgactg atcttaaagc aacacccagt gacacaacta 600 gccatttttt tctttgaata aaaaaatcca attatcattg tatttttttt atacaatgaa 660 aatttcacca aacaatgatt tgtggtattt ctgaagcaag tcatgttatg caaaattcta 720 taattcccat ttgacactac ggaagtaact gaagatctgc ttttacatgc gagacacatc 780 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 840 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 900 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 960 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 1020 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 1080 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 1140 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 1200 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 1260 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 1320 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 1380 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 1440 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 1500 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 1560 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 1620 ccaaaaatgg cttcctctat gctctcttcc gctactatgg ttgcctctcc ggctcaggcc 1680 actatggtcg ctcctttcaa cggacttaag tcctccgctg ccttcccagc cacccgcaag 1740 gctaacaacg acattacttc catcacaagc aacggcggaa gagttaactg catgcaggtg 1800 tggcctccga ttggaaagaa gaagtttgag actctctctt accttcctga ccttaccgat 1860 tccgaagcag cgaggctgca agagcagaaa ggaggtgaga tggacacgag gattgcgatc 1920 ataggcatga gcgccatact tccttgtgga accacggtga gagagtcatg ggagaccata 1980 agggctggca ttgattgcct gtctgatctt cctgaggatc gtgttgatgt gactgcgtac 2040 tttgacccag tcaagaccac aaaggacaaa atctactgca agagaggagg tttcattcct 2100 gagtatgact ttgatgcgcg tgagtttggt ctcaacatgt tccagatgga agattctgat 2160 gcgaatcaga ccatctcact tctcaaggtc aaagaagcac ttcaagatgc tgggattgat 2220 gcacttggaa aggagaagaa aaacatagga tgcgtgcttg gcattggagg tggacagaag 2280 agttcacatg agttctacag cagactcaac tatgtggttg tggagaaagt cttgagaaag 2340 atggggatgc cagaggaaga tgtcaaagtg gctgtggaga agtacaaagc gaacttccca 2400 gaatggagac ttgactcttt ccctggattc cttgggaatg tcactgctgg cagatgcacc 2460 aacacattca acttggatgg catgaactgc gttgtggatg ctgcatgtgc aagctccttg 2520 atagcagtca aggttgcgat tgatgaactg ctctatggtg actgcgacat gatggtcact 2580 ggtgcaacct gcacagacaa ctccattggg atgtacatgg ctttctcaaa gacacctgtg 2640 ttcagcacgg acccaagtgt gagagcgtat gatgagaaaa cgaaagggat gctcattggt 2700 gagggatcag ccatgttggt gctcaaacgt tatgcagatg cagtgaggga tggtgatgag 2760 attcatgctg tgattcgtgg ttgtgcaagc tcaagtgatg gcaaagcagc tggcatctac 2820 actcccacaa tcagtggtca agaggaagcc ttgagacgtg catacaatcg tgcctgcgtt 2880 gaccctgcca ctgtcacttt ggttgagggt catgggactg gcactccagt tggagatagg 2940 attgagttga ctgcgttgag gaacttgttt gacaaagcgt atggtgaagg gaacacagag 3000 aaagttgcag ttggaagtat caagtccagt attggtcatt tgaaggctgt tgctggtctt 3060 gctggaatga tcaaggtcat catggctctc aaacacaaga ctttgcctgg aacgatcaat 3120 gtggacaatc cacccaatct ctatgacaac actcccatca atgagtcatc cctctacatc 3180 aacacgatga acagaccctg gttccctcca cctggtgttc cgagacgtgc tggaatatcc 3240 agctttggct ttggaggtgc caactaccat gcagtgttgg aagaggctga accagagcac 3300 acgacagcgt acagactcaa caaacgtcca caaccggtgt tgatgatggc tgcaactcct 3360 gctgcgttgc agagcctttg tgaagctcag ctgaaagagt ttgaagctgc gatcaaggag 3420 aatgagacag tcaagaatac tgcctacatc aagtgtgtca agtttggaga acagttcaag 3480 ttccctggga gcatacctgc gaccaatgcg agactgggat tcttggtcaa agatgcggag 3540 gatgcgtgct ccacgcttcg tgctatatgt gctcagtttg ccaaagatgt gaccaaagaa 3600 gcgtggaggc ttccgagaga aggagtttct ttccgtgcca aaggcattgc caccaatggt 3660 gctgttgctg cactcttctc tggacaaggt gctcagtaca cacacatgtt cagtgaagtt 3720 gcgatgaact ggcctcagtt cagacagtcc attgctgcaa tggacgctgc acagtccaag 3780 gttgctggaa gtgacaaaga ctttgaaaga gtctctcaag tcctgtaccc aaggaaaccg 3840 tacgaacgtg agccagaaca agatcacaag aaaatctcac tcacagcata cagccagccg 3900 tcaaccctgg catgtgctct tggtgccttt gagattttca aggaagctgg attcactcca 3960 gactttgcag ctggacacag cttgggtgag tttgctgcgc tctatgcagc tggttgtgtt 4020 gacagagatg agttgtttga acttgtttgc agaagggcaa ggatcatggg aggcaaagat 4080 gcacctgcca cacccaaagg ttgcatggca gctgtcatag gacccaacgc agagaacatc 4140 aaggttcaag ctgccaacgt ctggcttgga aactcaaact ctccgtctca gactgtcatt 4200 actgggtctg ttgaagggat tcaagctgaa agtgccagat tgcagaagga agggttcaga 4260 gttgtgcctc ttgcttgtga gtctgccttc cattctccac agatggaaaa tgccagctca 4320 gctttcaaag atgtcatatc caaggtttcc ttccgtacac ccaaagcaga aacaaagctc 4380 ttcagcaatg tttctggaga gacctatccg acggatgcca gagagatgct cactcaacac 4440 atgacctcat ctgtgaagtt cctgacccaa gtgaggaaca tgcaccaagc tggtgcgaga 4500 atctttgttg agtttggacc gaaacaagtt ctcagcaaac ttgtttctga aaccctcaaa 4560 gatgacccgt ctgttgtcac agtgtctgtg aacccagctt ctggcacgga ctctgacata 4620 cagttgaggg atgctgcagt tcagctggtt gtggctggtg tcaatcttca agggtttgac 4680 aagtgggatg ctcctgatgc cacgaggatg caagccatca agaaaaagag aaccacactc 4740 agactttcag ctgccaccta tgtcagtgac aagaccaaga aagtgaggga tgctgccatg 4800 aacgacggaa gatgtgtcac atacctcaag ggtgctgcac cactcatcaa ggctcctgaa 4860 cctgtggttg acgaagctgc caagagggag gctgaaaggc ttcagaagga gcttcaagac 4920 gctcagaggc agcttgacga cgccaaacgt gcagctgcag aagccaactc caaacttgct 4980 gctgccaagg aggaagccaa gactgctgca gcttcagcca agcctgcagt tgacactgct 5040 gttgtggaga aacatcgtgc catcctcaag tccatgctgg ctgagctgga tggttatgga 5100 agtgttgatg cttcttccct tcagcaacag caacagcaac agactgctcc ggcgcctgtt 5160 aaagccgctg cccctgccgc tcccgttgca agtgctcctg ctcccgccgt gtccaacgag 5220 ttgcttgaga aagctgagac agttgtgatg gaggttctcg ctgccaagac tggatacgaa 5280 acagacatga ttgaggcgga catggagctt gagactgagt tgggaattga cagcatcaag 5340 agggttgaaa ttctttctga agtccaagct atgcttaacg ttgaggcgaa agatgttgat 5400 gctctctcta ggacgagaac agtgggtgaa gttgtcaacg ctatgaaggc agaaatcgct 5460 ggttcttccg ctccggcgcc ggcagctgcc gcacctgcac cggctaaagc ggctccagct 5520 gcagctgcac cagctgtttc taatgagctg ctcgaaaagg cagaaactgt tgtgatggaa 5580 gtccttgctg ccaaaactgg ctacgaaact gacatgatag aatcagacat ggagcttgag 5640 acggagcttg gaattgattc catcaagcgt gtggaaatcc tcagtgaagt ccaagccatg 5700 ctcaatgtgg aagctaagga tgtggatgct ctctcaagga ccagaactgt cggtgaggtt 5760 gtcaatgcca tgaaggctga gatagctgga ggctccgctc ctgcgccggc cgctgccgct 5820 cctggtcccg ctgccgcggc tccagctcca gctgcagccg ctccggccgt ctcaaacgaa 5880 ctccttgaaa aagctgagac cgtcgtgatg gaggtcttgg cagctaaaac cggctatgag 5940 actgacatga ttgagtctga catggaattg gagacagaac ttgggataga cagcatcaaa 6000 cgtgttgaaa tcctcagtga ggtccaagct atgctgaacg tggaagcgaa ggatgttgat 6060 gctctgtcca gaacgaggac cgttggtgag gttgtggatg ccatgaaggc tgaaattgct 6120 ggagggtctg cgcccgcgcc agctgcagcc gctcctgcac ccgcagccgc ggcacctgct 6180 ccagctgcac ctgcacctgc cgtttcttca gaactcttgg agaaggctga gacggttgtg 6240 atggaggttt tggctgcaaa gactggctat gagaccgaca tgattgaatc tgatatggaa 6300 ttggagacag agcttgggat cgattccatc aaaagagtgg agatcctgag tgaagtccaa 6360 gccatgctca atgttgaagc taaggatgtt gatgcacttt caagaacaag gacagtgggt 6420 gaggttgtgg atgccatgaa ggcggagatt gctggaggga gcgcgcctgc gccagctgcc 6480 gcggcacctg caccggcagc cgcagctcca gctccagctg caccagcacc ggccgcgccc 6540 gcgccagctg tttcatccga actcctggaa aaggccgaga cggttgtgat ggaggtgctc 6600 gcagctaaga ctggttacga gaccgacatg attgaaagtg acatggagct tgaaaccgag 6660 ttgggaatag atagcatcaa acgtgttgaa atcttgtctg aggtccaagc tatgttgaac 6720 gtggaggcaa aagatgtcga tgcgctttca agaaccagaa cagtcggtga ggtcgtggac 6780 gccatgaagg ctgaaattgc cggaagctct gctagtgcgc cagctgcagc cgcacctgca 6840 cctgctgccg cggcacctgc tccagctgca gccgctcctg ccgtcagcaa tgagttgctg 6900 gagaaagccg aaacagttgt gatggaagtc cttgctgcaa aaactggtta cgaaacggac 6960 atgattgagt cagacatgga attggagacc gaacttggaa tagattccat caagagagtg 7020 gagatcctct ctgaggtgca agccatgctc aacgttgagg ctaaagatgt tgatgcactc 7080 agtaggacaa gaactgtggg ggaagttgtc gatgcgatga aggctgagat tgctggaggc 7140 tctgctcctg cgccggcagc tgccgcacct gcacccgcag ccgcggcacc agctgtcagc 7200 aacgaattgc ttgagaaggc tgaaacagtt gtcatggaag tgctggctgc aaaaaccggc 7260 tatgagactg acatgattga gtctgacatg gaacttgaga cggaattggg cattgacagc 7320 atcaagcgtg ttgagatcct cagtgaagtc caagcaatgc tcaatgtgga ggctaaggat 7380 gtggatgccc tctcaaggac cagaacagtt ggtgaggtcg ttgatgcgat gaaggcggag 7440 atcgctggaa gttccgcgcc tgcgccagct gcagccgctc ctgcacccgc agccgcggca 7500 ccggctccag ctgcagccgc accggccgtc tcctcagaac tcctggaaaa ggctgagaca 7560 gttgtgatgg aagtccttgc tgcgaaaact ggctatgaga ccgacatgat tgaatctgac 7620 atggaactgg agaccgagct tggaattgac tccatcaaac gtgttgaaat cctctctgag 7680 gttcaagcga tgttgaatgt ggaggccaaa gatgttgatg ctctttccag aacaaggacg 7740 gtgggagagg tggttgatgc catgaaggct gagatagctg ggggttctgc tcctgcgcca 7800 gctgcagccg ctcctgctcc cgctgccgcg gcaccagctg tcagcaacga actccttgaa 7860 aaagctgaaa ctgttgtgat ggaggttttg gctgcaaaga ctggatacga gactgacatg 7920 atagagtcag acatggagtt ggaaacagag cttgggattg atagcatcaa gcgtgtggaa 7980 atcctttctg aggttcaagc catgctgaac gttgaggcca aagatgtcga tgctttgtca 8040 aggaccagaa cggttggaga agtggtcgat gccatgaagg ctgagatagc tggtggatca 8100 gcccctgctc cggctgcagc cgctcctgca agtgccggtg ctgcaccagc tgtgaagatt 8160 gattcagttc atggtgctga ctgtgacgat ttgtcactga tgcacgcaaa ggtggttgac 8220 atcagaaggc cagatgaact gattctggaa agaccggaga accgtccagt tcttgtcgtt 8280 gacgatggct cagagttgac acttgctctt gtgagggtgt tgggagcctg tgcagttgtc 8340 ttgacctttg agggattgca gttggctcag agagctggtg cagctgccat tcgtcatgtt 8400 ctggctaagg acctttcagc agaatcagct gagaaggcca tcaaagaggc tgagcaaaga 8460 tttggagcgt tgggaggctt cataagccag caagcagaaa ggtttgaacc agctgagata 8520 ctgggtttca cactcatgtg tgcgaagttt gccaaagcct ctctctgcac tgctgtggct 8580 ggaggtcgtc ctgccttcat cggtgtggct aggcttgacg gcagacttgg attcacctct 8640 caaggcactt ctgacgcact caagagagca cagcgtggag cgatctttgg actctgcaag 8700 acaatagggt tggagtggtc agaatctgat gtgttcagcc gtggtgtgga cattgctcaa 8760 gggatgcacc cagaagatgc tgcagttgcc atagttaggg agatggcgtg tgctgacatc 8820 agaatcagag aggttgggat tggagcaaac cagcaaagat gcacaatcag agctgccaag 8880 ttggagactg ggaaccctca gagacagata gcgaaagacg atgtgcttct ggtttctgga 8940 ggtgctcgtg ggatcacacc tctttgcatc cgtgagatca cgcgtcagat tgctggaggc 9000 aagtacattc tgttgggtcg tagcaaagtt tctgcgtctg aaccagcatg gtgtgctggc 9060 atcactgatg agaaggctgt gcagaaagct gccacccaag agttgaagag ggccttcagt 9120 gctggtgaag gacccaaacc cactccaagg gctgtcacga aacttgtggg atctgttctt 9180 ggtgctcgtg aagtgagaag ctccattgct gccattgaag ctctgggtgg caaagcgatc 9240 tacagctctt gtgatgtgaa ctctgctgca gatgttgcca aagcagttag ggatgcagag 9300 tcccagcttg gtgccagagt tagtggaatc gttcacgctt ctggtgtgct ccgtgatcgt 9360 ctcatagaga agaaactccc tgacgagttt gatgctgtct ttgggaccaa agtcactggg 9420 ttggagaatc ttctggctgc agttgatcgt gccaacctca aacacatggt tctcttcagc 9480 tcccttgctg gtttccacgg caatgttggt cagtctgact atgcgatggc aaacgaagct 9540 ctcaacaaga tgggtttgga acttgcgaag gatgtcagtg tcaagtccat ctgctttggt 9600 ccctgggatg gtgggatggt gacacctcaa ctcaagaaac agtttcaaga aatgggagtt 9660 cagatcattc ctcgtgaagg aggtgctgat acagttgcga ggattgtgtt gggatcaagt 9720 cctgctgaga tccttgttgg aaactggagg acaccatcca agaaagttgg aagtgacacg 9780 atcaccctgc atagaaagat cagtgccaag tccaatccct tcttggagga tcatgtcata 9840 caaggcagaa gggtgttgcc aatgaccctt gctattggga gccttgctga gacctgcctt 9900 ggactcttcc ctggctactc tctctgggcc atagatgacg cacagctctt caaaggtgtc 9960 actgttgatg gagacgtcaa ctgtgaagtg accctcacac cgtccactgc tccgagtggg 10020 agggtgaatg tccaagcgac gctcaagacg ttctcaagtg gaaaacttgt tccagcctac 10080 agagctgtca tagtgttgtc caaccaaggt gctccacctg ccaatgcaac catgcagcct 10140 ccgagccttg atgcagatcc tgctttgcaa gggtcagtct atgatggcaa gactctcttt 10200 catggtcctg cgttcagagg cattgatgac gttctcagct gcaccaagag ccagcttgtt 10260 gccaagtgtt ctgctgttcc tggatcagat gcagccagag gagagtttgc aactgacact 10320 gatgcacatg atccctttgt caatgatctt gcctttcaag caatgttggt ctgggtgagg 10380 agaacgctgg gacaagctgc actcccgaac tccattcaga ggatagtcca acacagaccg 10440 gtcccacaag acaaaccctt ctacatcacc ctcagatcca atcagagtgg tggacatagc 10500 cagcacaaac atgcacttca gttccacaac gaacaaggtg atctcttcat tgatgttcaa 10560 gcctctgtca ttgcgacaga ctcacttgcg ttctgagtag ttagcttaat cacttaggtc 10620 accagtatga actaaaatgc atgtaggtgt aagagctcat ggagagcatg gaatattgta 10680 tccgaccatg taacagtata ataactgagc tccatctcac ttcttctatg aataaacaaa 10740 ggatgttatg atatattaac actctatcta tgcaccttat tgttctatga taaatttcct 10800 cttattatta taaatcatct gaatcgtgac ggcttatgga atgcttcaaa tagtacaaaa 10860 acaaatgtgt actataagac tttctaaaca attctaactt tagcattgtg aacgagacat 10920 aagtgttaag aagacataac aattataatg gaagaagttt gtctccattt atatattata 10980 tattacccac ttatgtatta tattaggatg ttaaggagac ataacaatta taaagagaga 11040 agtttgtatc catttatata ttatatacta cccatttata tattatactt atccacttat 11100 ttaatgtctt tataaggttt gatccatgat atttctaata ttttagttga tatgtatatg 11160 aaaaggtact atttgaactc tcttactctg tataaaggtt ggatcatcct taaagtgggt 11220 ctatttaatt ttattgcttc ttacagataa aaaaaaaatt atgagttggt ttgataaaat 11280 attgaaggat ttaaaataat aataaataat aaataacata taatatatgt atataaattt 11340 attataatat aacatttatc tataaaaaag taaatattgt cataaatcta tacaatcgtt 11400 tagccttgct ggaacgaatc tcaattattt aaacgagagt aaacatattt gactttttgg 11460 ttatttaaca aattattatt taacactata tgaaattttt tttttttatc agcaaagaat 11520 aaaattaaat taagaaggac aatggtgtcc caatccttat acaaccaact tccacaagaa 11580 agtcaagtca gagacaacaa aaaaacaagc aaaggaaatt ttttaatttg agttgtcttg 11640 tttgctgcat aatttatgca gtaaaacact acacataacc cttttagcag tagagcaatg 11700 gttgaccgtg tgcttagctt cttttatttt atttttttat cagcaaagaa taaataaaat 11760 aaaatgagac acttcaggga tgtttcaacc cttatacaaa accccaaaaa caagtttcct 11820 agcaccctac caacgaattc gcggccgctt aattaagatg agtgatactc aggactcagg 11880 actcactctg ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt 11940 tgtctagacc taggctcagc aactcatcca gtcaagttcg agtcagtgag ttgggcggcc 12000 gcctcccagt atcattatag tgaaagtttt ggctctctcg ccggtggttt tttacctcta 12060 tttaaagggg ttttccacct aaaaattctg gtatcattct cactttactt gttactttaa 12120 tttctcataa tctttggttg aaattatcac gcttccgcac acgatatccc tacaaattta 12180 ttatttgtta aacattttca aaccgcataa aattttatga agtcccgtct atctttaatg 12240 tagtctaaca ttttcatatt gaaatatata atttacttaa ttttagcgtt ggtagaaagc 12300 ataatgattt attcttattc ttcttcatat aaatgtttaa tatacaatat aaacaaattc 12360 tttaccttaa gaaggatttc ccattttata ttttaaaaat atatttatca aatatttttc 12420 aaccacgtaa atctcataat aataagttgt ttcaaaagta ataaaattta actccataat 12480 ttttttattc gactgatctt aaagcaacac ccagtgacac aactagccat ttttttcttt 12540 gaataaaaaa atccaattat cattgtattt tttttataca atgaaaattt caccaaacaa 12600 tgatttgtgg tatttctgaa gcaagtcatg ttatgcaaaa ttctataatt cccatttgac 12660 actacggaag taactgaaga tctgctttta catgcgagac acatcttcta aagtaatttt 12720 aataatagtt actatattca agatttcata tatcaaatac tcaatattac ttctaaaaaa 12780 ttaattagat ataattaaaa tattactttt ttaattttaa gtttaattgt tgaatttgtg 12840 actattgatt tattattcta ctatgtttaa attgttttat agatagttta aagtaaatat 12900 aagtaatgta gtagagtgtt agagtgttac cctaaaccat aaactataag atttatggtg 12960 gactaatttt catatatttc ttattgcttt taccttttct tggtatgtaa gtccgtaact 13020 ggaattactg tgggttgcca tgacactctg tggtcttttg gttcatgcat ggatcttgcg 13080 caagaaaaag acaaagaaca aagaaaaaag acaaaacaga gagacaaaac gcaatcacac 13140 aaccaactca aattagtcac tggctgatca agatcgccgc gtccatgtat gtctaaatgc 13200 catgcaaagc aacacgtgct taacatgcac tttaaatggc tcacccatct caacccacac 13260 acaaacacat tgcctttttc ttcatcatca ccacaaccac ctgtatatat tcattctctt 13320 ccgccacctc aatttcttca cttcaacaca cgtcaacctg catatgcgtg tcatcccatg 13380 cccaaatctc catgcatgtt ccaaccacct tctctcttat ataataccta taaatacctc 13440 taatatcact cacttctttc atcatccatc catccagagt actactactc tactactata 13500 ataccccaac ccaactcata ttcaatacta ctctaggatc cacaaccaaa aatggcttcc 13560 tctatgctct cttccgctac tatggttgcc tctccggctc aggccactat ggtcgctcct 13620 ttcaacggac ttaagtcctc cgctgccttc ccagccaccc gcaaggctaa caacgacatt 13680 acttccatca caagcaacgg cggaagagtt aactgcatgc aggtgtggcc tccgattgga 13740 aagaagaagt ttgagactct ctcttacctt cctgacctta ccgattccga agctgcaagg 13800 aacgtgagtg cggctcatga gatgcatgac gagaagagga tagctgttgt gggcatggct 13860 gttcagtatg ctggatgcaa aacgaaagac gagttctggg aagttctcat gaatggaaaa 13920 gttgagagca aagtgatcag cgacaaacgt cttggttcaa attaccgtgc tgaacactac 13980 aaagctgaaa ggagcaagta tgctgacacc ttctgcaatg agacatatgg aactcttgat 14040 gagaacgaaa tagacaatga gcatgaactc cttttgaacc ttgccaaaca agccttggct 14100 gaaacatctg tcaaagattc aacaagatgt ggcatagttt ctggatgcct cagcttcccg 14160 atggacaacc tccaaggtga actcttgaat gtgtaccaga accatgttga gaagaaactt 14220 ggtgccagag ttttcaaaga tgcctctcac tggtcagaaa gagaacagtc caacaaacca 14280 gaggctggtg acagaagaat cttcatggac cctgcttcct ttgttgctga ggaactcaat 14340 cttggcgctc tccactactc tgttgatgct gcctgtgcca ctgcgctcta tgtgctcaga 14400 ctggcgcaag atcacttggt ctctggtgca gctgatgtga tgctttgtgg tgcaacatgc 14460 ctgccggagc cgtttttcat actctctggg ttctcaacct tccaagccat gcctgttgga 14520 acgggtcaga atgtgtccat gccgctgcac aaagattctc aaggattgac ccctggtgaa 14580 ggaggttcca tcatggtcct caaacgtttg gatgacgcaa tcagagatgg agatcacatc 14640 tatggaacac ttttgggagc caatgtcagc aacagtggca ctggtctgcc acttaaaccc 14700 cttttgccgt cagagaaaaa gtgcctcatg gacacctaca cacgtatcaa tgtgcatccc 14760 cacaagattc agtatgttga atgccatgca actgggactc cacaaggtga tcgtgtggaa 14820 attgatgctg tgaaggcttg ctttgaaggc aaagtcccac gttttggaac cacaaaaggg 14880 aactttggac acacattggt ggcagctggg tttgctggca tgtgcaaagt gcttctctcc 14940 atgaagcacg ggatcattcc acccactccg ggaatagatg acgaaacgaa gatggaccct 15000 ctggttgtct ctggtgaggc cattccctgg ccagaaacga atggagaacc gaagcgtgct 15060 ggtctctcag cgtttggatt tggtgggacc aatgctcatg ctgtctttga ggaacatgac 15120 ccaagcaatg ctgcatgcac tggccatgac tccatttctg ctctctctgc acgttgtggt 15180 ggtgagtcaa acatgagaat agcaatcact gggatggatg ccacttttgg tgctttgaaa 15240 ggattggatg cttttgaaag ggccatctac actggtgctc atggtgctat accactccca 15300 gaaaagagat ggagattcct ggggaaagac aaggacttcc ttgatctttg tggagtcaaa 15360 gcaacaccgc atggctgtta cattgaagat gttgaagttg atttccagag gctgaggact 15420 ccaatgactc ctgaagatat gctcttgcct caacagcttc tggctgtcac cacaattgat 15480 agggccatat tggactctgg catgaagaaa ggtggaaatg tggctgtctt tgtggggttg 15540 gggaccgatc ttgaactcta ccgtcaccgt gcaagggttg ctctcaagga gagagtgagg 15600 cctgaagcca gcaagaaact caatgacatg atgcagtaca tcaatgattg tgggacaagc 15660 acttcttaca ccagctacat aggcaacttg gttgcaacaa gggtcagttc acaatggggc 15720 ttcacgggac cttccttcac aatcacagaa ggcaacaatt ctgtgtacag atgtgctgaa 15780 cttggcaaat acctcttgga aacgggagag gttgatgggg ttgtggttgc gggtgttgat 15840 ctctgtggga gtgctgagaa cctttacgtc aagtcaaggc gtttcaaggt gtctacatct 15900 gacaccccga gagccagctt tgatgcagct gctgatggtt actttgttgg agagggctgt 15960 ggtgcatttg tgctcaagcg tgagacaagc tgcaccaagg atgacagaat ctatgcttgc 16020 atggatgcca tagttcctgg aaatgttcca tctgcatgtt tgagagaggc attggatcaa 16080 gccagagtca agcctggaga catcgagatg ttggagcttt ctgctgattc agcgaggcat 16140 ttgaaggacc cgtctgttct cccaaaggaa ctcactgctg aggaagagat tggcggactt 16200 cagacgattc ttcgtgacga tgacaagctc ccaaggaatg ttgcaactgg aagtgtcaag 16260 gccactgttg gtgacactgg ttatgcttct ggtgctgcca gtttgattaa ggctgcgctt 16320 tgcatctaca accgttacct gcccagcaat ggagatgact gggatgaacc tgcacctgaa 16380 gcaccctggg acagcacact ctttgcgtgc cagacctcaa gagcttggtt gaagaaccct 16440 ggtgagagac gttatgcggc tgtttccgga gtcagtgaaa ccagaagttg ctacagtgtg 16500 ctgctttctg aagcagaggg acactacgag agagagaaca gaatcagttt ggatgaggaa 16560 gcacccaagc tcattgtgtt gagagcagac tcccatgaag agattcttgg aagattggac 16620 aagataaggg aaaggttctt gcagcccacg ggtgctgcac ccagagagtc tgaattgaag 16680 gcgcaagccc gtaggatctt tcttgaactc cttggtgaga cccttgccca agatgcagct 16740 tcatctggaa gtcagaagcc ccttgctctc agcttggtct ccactccatc caagctccag 16800 agagaggtgg aacttgcggc aaaaggcatt ccgagatgtc tcaagatgag acgtgattgg 16860 agttcccctg ctggaagcag atatgcgcct gagcctttgg ccagtgacag agttgccttc 16920 atgtatggag aaggaaggag cccttactat ggcatcacac aagacattca taggatttgg 16980 cctgaacttc atgaggtcat caatgagaaa acaaaccgtc tctgggctga aggtgacaga 17040 tgggtcatgc caagggccag cttcaagagt gagcttgagt cacaacagca agagtttgac 17100 agaaacatga ttgaaatgtt ccgtcttgga atcctcacct caattgcctt cacaaacctg 17160 gcgagagatg ttctcaacat cacgcccaaa gcagcctttg gactcagttt gggagagatt 17220 tccatgatct ttgctttctc caagaaaaac ggtttgataa gcgatcagtt gacaaaggat 17280 ttgagagagt cagatgtctg gaacaaagct ctggctgttg agttcaatgc gctcagagaa 17340 gcctggggca ttccacagtc tgtcccaaaa gatgagttct ggcaaggtta catagtgagg 17400 ggaacgaaac aagacataga agcagccata gcaccagact caaagtatgt gaggttgacc 17460 atcatcaatg atgccaacac tgctctcatc agtggaaagc ctgatgcatg caaagctgcg 17520 attgctcgtc ttggtggcaa catccctgcg cttccagtca ctcaaggcat gtgtgggcat 17580 tgcccagagg tgggacctta caccaaggac atcgcaaaga tccacgccaa ccttgagttc 17640 cctgtggttg atggtcttga tctctggact accataaacc agaagaggct tgtgccaaga 17700 gccacgggtg ccaaagatga atgggctcct tcatcctttg gggagtatgc tggacagctc 17760 tatgagaaac aagccaactt ccctcagata gttgaaacca tctacaagca gaactatgat 17820 gtgtttgttg aagttgggcc aaacaatcac agaagcacag cagtgagaac cacgcttgga 17880 ccacagagaa accaccttgc tggtgccata gacaagcaga acgaggatgc ttggacgaca 17940 atagtcaagc tcgtggcttc attgaaggct caccttgttc ctggagtgac catcagtccg 18000 ctctaccact ccaagctcgt tgcagaggct gaggcttgtt atgctgcgct ctgtaaaggt 18060 gagaaaccca agaaaaacaa gtttgtgaga aagattcagt tgaatggcag attcaacagc 18120 aaggcagacc ccatctcctc agcagacctt gcatccttcc cacctgcgga ccctgcgatt 18180 gaagctgcca tctcttcaag gatcatgaag cctgtggctc cgaaattcta tgcgcgtctc 18240 aacattgatg agcaagatga gaccagagat ccgatcttga acaaggacaa tgcgccttcc 18300 agctccagct cttccagcag ttcctcttcc agcagttcat ctccgtctcc tgctccttca 18360 gctccagttc agaagaaagc tgccccagct gcggagacaa aagctgttgc cagtgctgat 18420 gcacttcgta gtgccctgct tgatcttgac tccatgcttg cgctcagctc tgcaagtgct 18480 tctggcaacc ttgttgagac tgcgccatct gatgccagtg tcattgtccc tccgtgcaac 18540 attgctgatc ttggcagcag agctttcatg aaaacttatg gtgtttctgc gcctctgtac 18600 accggtgcaa tggccaaggg aattgcctct gcggacttgg tgatcgcagc tggaaggcaa 18660 ggaatccttg cgtcatttgg tgctggaggc cttccaatgc aagttgtgcg tgagtccatt 18720 gaaaagattc aagctgccct gcccaatgga ccatatgctg tcaacctcat ccattctcct 18780 tttgacagca accttgagaa gggaaatgtt gatctcttct tggaaaaggg tgtcactttt 18840 gttgaggctt cagccttcat gacgttgaca ccgcaagttg tcagatacag agcggctggg 18900 ttgacgagga atgcagatgg gtcagtcaac ataaggaaca gaatcattgg gaaagtctcc 18960 agaactgagc tggctgaaat gttcatgcgt cctgcacctg agcatctttt gcagaagctc 19020 attgcttctg gggagatcaa tcaagaacaa gctgagttgg ccagacgtgt tcctgtggct 19080 gatgacattg cggttgaagc tgattctggt ggacacactg acaaccgtcc aatccatgtc 19140 attctgcctc tgattatcaa cctgagggat aggcttcaca gagagtgtgg atacccagca 19200 aacctgaggg ttcgtgttgg tgctgggggt ggcattgggt gtccccaagc tgcgctggca 19260 actttcaaca tgggtgcctc tttcattgtc actggaacag tgaaccaagt tgccaaacaa 19320 tctgggacgt gtgacaatgt gaggaaacag cttgcgaaag caacttactc agatgtttgc 19380 atggcaccgg cagctgacat gtttgaggaa ggtgtgaaac ttcaagtttt gaagaaagga 19440 acaatgttcc cttcaagagc caacaaactc tatgagttgt tctgcaagta tgattctttt 19500 gagtccatgc ctccagcaga gcttgcgagg gttgagaaaa gaatcttcag tcgtgcattg 19560 gaagaggtct gggatgagac caagaacttc tacatcaacc gtctccacaa cccagagaag 19620 atacagaggg ctgagaggga ccccaaactg aagatgtcac tgtgtttcag atggtatctg 19680 tccctggctt caaggtgggc caacactgga gcttctgaca gagtcatgga ctaccaagtt 19740 tggtgtggtc ctgccattgg ttcattcaat gatttcatca aaggaactta ccttgatccg 19800 gcagtggcaa atgagtaccc gtgtgttgtg cagatcaaca aacagattct tagaggagcg 19860 tgcttcttga ggcgtcttga aatactgaga aatgcaaggc tttcagatgg tgcagctgca 19920 cttgtggcga gcattgatga cacatatgtc cctgctgaga agctgtgagt agttagctta 19980 atcacttagg tcaccagtat gaactaaaat gcatgtaggt gtaagagctc atggagagca 20040 tggaatattg tatccgacca tgtaacagta taataactga gctccatctc acttcttcta 20100 tgaataaaca aaggatgtta tgatatatta acactctatc tatgcacctt attgttctat 20160 gataaatttc ctcttattat tataaatcat ctgaatcgtg acggcttatg gaatgcttca 20220 aatagtacaa aaacaaatgt gtactataag actttctaaa caattctaac tttagcattg 20280 tgaacgagac ataagtgtta agaagacata acaattataa tggaagaagt ttgtctccat 20340 ttatatatta tatattaccc acttatgtat tatattagga tgttaaggag acataacaat 20400 tataaagaga gaagtttgta tccatttata tattatatac tacccattta tatattatac 20460 ttatccactt atttaatgtc tttataaggt ttgatccatg atatttctaa tattttagtt 20520 gatatgtata tgaaaaggta ctatttgaac tctcttactc tgtataaagg ttggatcatc 20580 cttaaagtgg gtctatttaa ttttattgct tcttacagat aaaaaaaaaa ttatgagttg 20640 gtttgataaa atattgaagg atttaaaata ataataaata ataaataaca tataatatat 20700 gtatataaat ttattataat ataacattta tctataaaaa agtaaatatt gtcataaatc 20760 tatacaatcg tttagccttg ctggaacgaa tctcaattat ttaaacgaga gtaaacatat 20820 ttgacttttt ggttatttaa caaattatta tttaacacta tatgaaattt ttttttttta 20880 tcagcaaaga ataaaattaa attaagaagg acaatggtgt cccaatcctt atacaaccaa 20940 cttccacaag aaagtcaagt cagagacaac aaaaaaacaa gcaaaggaaa ttttttaatt 21000 tgagttgtct tgtttgctgc ataatttatg cagtaaaaca ctacacataa cccttttagc 21060 agtagagcaa tggttgaccg tgtgcttagc ttcttttatt ttattttttt atcagcaaag 21120 aataaataaa ataaaatgag acacttcagg gatgtttcaa cccttataca aaaccccaaa 21180 aacaagtttc ctagcaccct accaacgaat tcgcggccgc ttaattaatc tgacctgacc 21240 actagacctg agtgagtaca gatgtgctac tagtgctagc ctcgaggtcg accaactttt 21300 ctatacaaag ttgtctagac ctaggactag gcactgagtg actcactgac ctgactgagt 21360 gactggcggc cgcctcccag tatcattata gtgaaagttt tggctctctc gccggtggtt 21420 ttttacctct atttaaaggg gttttccacc taaaaattct ggtatcattc tcactttact 21480 tgttacttta atttctcata atctttggtt gaaattatca cgcttccgca cacgatatcc 21540 ctacaaattt attatttgtt aaacattttc aaaccgcata aaattttatg aagtcccgtc 21600 tatctttaat gtagtctaac attttcatat tgaaatatat aatttactta attttagcgt 21660 tggtagaaag cataatgatt tattcttatt cttcttcata taaatgttta atatacaata 21720 taaacaaatt ctttacctta agaaggattt cccattttat attttaaaaa tatatttatc 21780 aaatattttt caaccacgta aatctcataa taataagttg tttcaaaagt aataaaattt 21840 aactccataa tttttttatt cgactgatct taaagcaaca cccagtgaca caactagcca 21900 tttttttctt tgaataaaaa aatccaatta tcattgtatt ttttttatac aatgaaaatt 21960 tcaccaaaca atgatttgtg gtatttctga agcaagtcat gttatgcaaa attctataat 22020 tcccatttga cactacggaa gtaactgaag atctgctttt acatgcgaga cacatcttct 22080 aaagtaattt taataatagt tactatattc aagatttcat atatcaaata ctcaatatta 22140 cttctaaaaa attaattaga tataattaaa atattacttt tttaatttta agtttaattg 22200 ttgaatttgt gactattgat ttattattct actatgttta aattgtttta tagatagttt 22260 aaagtaaata taagtaatgt agtagagtgt tagagtgtta ccctaaacca taaactataa 22320 gatttatggt ggactaattt tcatatattt cttattgctt ttaccttttc ttggtatgta 22380 agtccgtaac tggaattact gtgggttgcc atgacactct gtggtctttt ggttcatgca 22440 tggatcttgc gcaagaaaaa gacaaagaac aaagaaaaaa gacaaaacag agagacaaaa 22500 cgcaatcaca caaccaactc aaattagtca ctggctgatc aagatcgccg cgtccatgta 22560 tgtctaaatg ccatgcaaag caacacgtgc ttaacatgca ctttaaatgg ctcacccatc 22620 tcaacccaca cacaaacaca ttgccttttt cttcatcatc accacaacca cctgtatata 22680 ttcattctct tccgccacct caatttcttc acttcaacac acgtcaacct gcatatgcgt 22740 gtcatcccat gcccaaatct ccatgcatgt tccaaccacc ttctctctta tataatacct 22800 ataaatacct ctaatatcac tcacttcttt catcatccat ccatccagag tactactact 22860 ctactactat aataccccaa cccaactcat attcaatact actctaggat ccacaaccaa 22920 aaatggcttc ctctatgctc tcttccgcta ctatggttgc ctctccggct caggccacta 22980 tggtcgctcc tttcaacgga cttaagtcct ccgctgcctt cccagccacc cgcaaggcta 23040 acaacgacat tacttccatc acaagcaacg gcggaagagt taactgcatg caggtgtggc 23100 ctccgattgg aaagaagaag tttgagactc tctcttacct tcctgacctt accgattccg 23160 aagcgcttcg tgtcaagacc aacaagaagc catgttggga gatgaccaag gaggaactga 23220 cctctggcaa aactgaggtg ttcaactatg aggaactcct ggagtttgca gagggtgaca 23280 tagctaaggt ttttggtccg gagtttgctg tcatagacaa gtaccccaga agggtgaggc 23340 tgcctgctcg tgaatacctg cttgtgacaa gggtcacact catggatgcc gaggtcaaca 23400 actacagagt tggtgccaga atggtcactg agtatgatct tccagtgaat ggcgaattgt 23460 ctgagggagg tgattgtccc tgggcagttc tggttgagag tggacagtgc gatttgatgc 23520 tgatatcata catgggaatt gatttccaga accaaggtga cagagtttac cgtctgctca 23580 acaccacgtt gacattctat ggggttgccc atgagggaga gacccttgag tatgacatca 23640 gagtgaccgg ttttgccaaa cgtttggatg gtggcatctc catgttcttc tttgagtatg 23700 actgctatgt caatgggagg cttctcattg agatgagaga tggctgtgct ggcttcttca 23760 caaatgagga gcttgatgcg ggaaagggtg tggtgttcac aagaggggat ttggctgcca 23820 gagctaagat tccaaagcaa gatgtttctc cctatgctgt cgctccctgc cttcacaaaa 23880 caaaactcaa cgaaaaggag atgcagacct tggttgacaa agattgggct tccgtttttg 23940 gctcaaagaa tggcatgccg gagatcaact acaaactctg cgcacgtaag atgctcatga 24000 ttgatagagt caccagcatt gaccataagg gaggtgtgta cggtttgggt cagttggttg 24060 gtgagaaaat cttggagagg gaccattggt acttcccatg ccattttgtt aaggatcaag 24120 tcatggctgg atctcttgtc agcgacggtt gttcacaaat gctcaagatg tacatgatct 24180 ggctcggttt gcaccttacc accggaccat ttgacttcag acccgtcaac ggacacccga 24240 acaaagttag gtgtaggggt cagatatctc ctcataaggg aaagttggtc tacgtcatgg 24300 agatcaaaga gatgggattc gacgaggata acgatccgta cgccattgct gacgtcaaca 24360 tcattgacgt tgatttcgaa aagggtcaag atttcagcct tgatagaatc agcgactacg 24420 gaaaggggga tcttaacaaa aagatcgtcg tggacttcaa gggcattgct ctcaagatgc 24480 agaagagatc cacgaacaag aacccctcca aagttcagcc cgtttttgct aacggtgcag 24540 ccactgtggg accggaagcg tccaaggcca gctctggtgc cagtgctagt gcttctgctg 24600 cccctgcgaa gcctgctttc tcagcagatg ttcttgcgcc aaagccagtt gcacttcctg 24660 aacatatcct caagggtgac gctctcgctc cgaaagaaat gtcctggcat cccatgtcaa 24720 aacttcctgg caacccgacg ccgttcttct cccctagttc ttaccctccg agggcaattt 24780 gcttcatccc tttccctggc aacccacttg acaacaattg caaagctgga gaaatgcctc 24840 tgaactggta caacatgtca gagttcatgt gtggaaaagt tagcaactgc ttgggaccag 24900 agttcgcaag atttgataag tcaaatacga gcagaagccc tgcttttgat ttggctcttg 24960 tgactagagt tgttgaagtc acaaacatgg aacatggcaa gttcctgaat gttgattgca 25020 atcccagcaa aggcacaatg gtgggggagt ttgactgtcc tcaagatgcg tggttctttg 25080 atggttcttg caatgatggg cacatgcctt attccatcat catggaaatc ggacttcaga 25140 catccggtgt tctcaccagt gtgttgaaag caccactgac gatggacaaa gatgacattc 25200 tctttaggaa ccttgatgca agtgctgaaa tggtgaggcc agatgtggat gtgaggggga 25260 aaaccataag gaatgtgacc aagtgcactg gatatgcaat gttgggaaag atggggattc 25320 acagattcac gtttgagttg tctgttgatg gcgtggtttt ctacaaagga agcacttcct 25380 ttggatggtt cactccagag gtgtttgctc agcaagctgg acttgacaat gggaaaaaga 25440 cggagccttg gtgcaaaact aacaacacga gtgtgagaag agttgaaatc gcatcagcca 25500 aaggcaaaga acagctgact gagaaacttc cagatgcaac taatgctcaa gttctcagaa 25560 ggtcagaaca gtgtgagtat ttggattacc tcaacattgc tcctgattct gggctgcatg 25620 ggaaaggata tgcgcatgga cacaaagatg ttaacccaca agactggttc ttctcttgtc 25680 acttttggtt tgatcctgtg atgcctggat ctttgggaat tgagtcaatg ttccagctca 25740 ttgaggcatt tgcggtggac cagaacatac cgggagaata caatgtgagc aatccgacct 25800 ttgcccatgc ccctggaaaa acggcgtgga agtaccgtgg tcaattgaca ccaaagaacc 25860 gtgcgatgga ctgtgaggtg cacatagttt caatcacagc ctctcctgaa aatggaggct 25920 acgttgacat agtggcagat ggagcacttt gggttgatgg tctccgtgtg tatgaagcca 25980 aagaactcag agttcgtgtt gtcagtgcaa aacctcaagc cattccggat gttcaacaac 26040 agccacccag cgcaaaagcg gaccctggga aaactggagt tgcactcagt ccgactcagt 26100 tgagagatgt cctgcttgaa gtggacaatc cattgtatct tggtgttgaa aactccaatt 26160 tggtgcagtt tgaatccaaa cctgcaactt cttcaaggat tgtcagtatc aaaccatgct 26220 ccataagtga tcttggtgac aagtccttca tggaaacgta caatgtgtca gcacctctgt 26280 acactggagc aatggccaag ggaattgcgt ctgcggacct tgtcattgct gctgggaaga 26340 ggaagatatt gggttctttt ggagcgggtg ggcttccaat gcaccatgtg agggcagctt 26400 tggagaagat acaagcggct ctgcctcaag gaccatatgc tgtgaacctc attcattcac 26460 cttttgacag caacttggaa aaggggaatg ttgatctctt cctcgaaaag ggcgttactg 26520 tggtggaggc ttctgcattc atgaccctca ctccccaagt tgttcgttac cgtgcagccg 26580 gactctcaag gaacgctgat gggagtgtca acatcagaaa ccgtatcatt ggaaaagtgt 26640 ccagaacaga actcgccgaa atgttcatca gaccggctcc ggaacacctc cttgagaaac 26700 tcatagcttc tggtgagata acacaagaac aagccgaact tgcccgtcgt gttcctgtcg 26760 cagacgacat cgctgttgaa gctgattctg gtggtcacac tgacaaccgt cccatccacg 26820 tcatcttgcc tttgatcatc aaccttagga acagactgca cagagagtgt ggttatcctg 26880 cgcaccttag agtcagagtt ggagctggag gcggtgttgg ctgcccacaa gctgctgcgg 26940 ctgccctcac aatgggagct gccttcatag tgactgggac tgtcaatcaa gttgctaaac 27000 agtccgggac atgtgacaac gtgagaaaac aactcagtca agccacctac agtgacattt 27060 gcatggctcc agcagctgat atgtttgagg aaggtgtgaa actccaagtt ctcaagaagg 27120 gaacaatgtt cccatcaaga gctaacaaat tgtacgaact cttttgcaag tacgactctt 27180 tcgattcaat gcctcctgct gaacttgaaa ggatcgagaa gaggatcttc aagagggcac 27240 ttcaagaggt ctgggaagag accaaggact tttacatcaa tggtttgaag aatccagaga 27300 agatacagag agcggaacat gaccccaaac tcaagatgtc tttgtgtttc agatggtatc 27360 ttggtcttgc atcacgttgg gccaacatgg gtgctcctga tcgtgtcatg gactatcaag 27420 tctggtgtgg tccagccatt ggagccttca atgacttcat caaaggaaca tatcttgatc 27480 ctgctgtctc taatgagtac ccatgtgttg tccagatcaa ccttcagatt ttgcgtggtg 27540 catgttacct gaggcgtctt aatgctctga gaaatgatcc gaggattgat cttgagactg 27600 aagatgctgc ctttgtctat gagccaacga atgcgctttg agtagttagc ttaatcactt 27660 aggtcaccag tatgaactaa aatgcatgta ggtgtaagag ctcatggaga gcatggaata 27720 ttgtatccga ccatgtaaca gtataataac tgagctccat ctcacttctt ctatgaataa 27780 acaaaggatg ttatgatata ttaacactct atctatgcac cttattgttc tatgataaat 27840 ttcctcttat tattataaat catctgaatc gtgacggctt atggaatgct tcaaatagta 27900 caaaaacaaa tgtgtactat aagactttct aaacaattct aactttagca ttgtgaacga 27960 gacataagtg ttaagaagac ataacaatta taatggaaga agtttgtctc catttatata 28020 ttatatatta cccacttatg tattatatta ggatgttaag gagacataac aattataaag 28080 agagaagttt gtatccattt atatattata tactacccat ttatatatta tacttatcca 28140 cttatttaat gtctttataa ggtttgatcc atgatatttc taatatttta gttgatatgt 28200 atatgaaaag gtactatttg aactctctta ctctgtataa aggttggatc atccttaaag 28260 tgggtctatt taattttatt gcttcttaca gataaaaaaa aaattatgag ttggtttgat 28320 aaaatattga aggatttaaa ataataataa ataataaata acatataata tatgtatata 28380 aatttattat aatataacat ttatctataa aaaagtaaat attgtcataa atctatacaa 28440 tcgtttagcc ttgctggaac gaatctcaat tatttaaacg agagtaaaca tatttgactt 28500 tttggttatt taacaaatta ttatttaaca ctatatgaaa tttttttttt ttatcagcaa 28560 agaataaaat taaattaaga aggacaatgg tgtcccaatc cttatacaac caacttccac 28620 aagaaagtca agtcagagac aacaaaaaaa caagcaaagg aaatttttta atttgagttg 28680 tcttgtttgc tgcataattt atgcagtaaa acactacaca taaccctttt agcagtagag 28740 caatggttga ccgtgtgctt agcttctttt attttatttt tttatcagca aagaataaat 28800 aaaataaaat gagacacttc agggatgttt caacccttat acaaaacccc aaaaacaagt 28860 ttcctagcac cctaccaacg aattcgcggc cgcttaatta atcacgagtg gatgactgtc 28920 acgactgact catgactgac tactagtgct agcctcgagg tcgacacaac tttgtataat 28980 aaagttgtct agacctagga gctgtgctgg gtaactcaca tgagtcactg gtcacatcag 29040 cggccgcctc ccagtatcat tatagtgaaa gttttggctc tctcgccggt ggttttttac 29100 ctctatttaa aggggttttc cacctaaaaa ttctggtatc attctcactt tacttgttac 29160 tttaatttct cataatcttt ggttgaaatt atcacgcttc cgcacacgat atccctacaa 29220 atttattatt tgttaaacat tttcaaaccg cataaaattt tatgaagtcc cgtctatctt 29280 taatgtagtc taacattttc atattgaaat atataattta cttaatttta gcgttggtag 29340 aaagcataat gatttattct tattcttctt catataaatg tttaatatac aatataaaca 29400 aattctttac cttaagaagg atttcccatt ttatatttta aaaatatatt tatcaaatat 29460 ttttcaacca cgtaaatctc ataataataa gttgtttcaa aagtaataaa atttaactcc 29520 ataatttttt tattcgactg atcttaaagc aacacccagt gacacaacta gccatttttt 29580 tctttgaata aaaaaatcca attatcattg tatttttttt atacaatgaa aatttcacca 29640 aacaatgatt tgtggtattt ctgaagcaag tcatgttatg caaaattcta taattcccat 29700 ttgacactac ggaagtaact gaagatctgc ttttacatgc gagacacatc ttctaaagta 29760 attttaataa tagttactat attcaagatt tcatatatca aatactcaat attacttcta 29820 aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta attgttgaat 29880 ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata gtttaaagta 29940 aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact ataagattta 30000 tggtggacta attttcatat atttcttatt gcttttacct tttcttggta tgtaagtccg 30060 taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca tgcatggatc 30120 ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac aaaacgcaat 30180 cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca tgtatgtcta 30240 aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc catctcaacc 30300 cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta tatattcatt 30360 ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat gcgtgtcatc 30420 ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat acctataaat 30480 acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac tactctacta 30540 ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa ccaaaaatgg 30600 cttcctctat gctctcttcc gctactatgg ttgcctctcc ggctcaggcc actatggtcg 30660 ctcctttcaa cggacttaag tcctccgctg ccttcccagc cacccgcaag gctaacaacg 30720 acattacttc catcacaagc aacggcggaa gagttaactg catgcaggtg tggcctccga 30780 ttggaaagaa gaagtttgag actctctctt accttcctga ccttaccgat tccgaacttc 30840 agcacacttg gcttccgaag cctcccaatc tgaccctctt gtcagatgag gttcatctct 30900 ggaggattcc tcttgaccag cctgagtcac aacttcaaga ccttgctgcc accctgagca 30960 gtgatgaatt ggcgagggca aacagattct actttccaga acacagaagg cgtttcactg 31020 ctgggagagg catcctcaga tccatcttgg gtggatactt gggagtggaa ccgggtcaag 31080 tcaagtttga ttatgagtcc cgtgggaaac cgatccttgg tgacagattt gctgagagtg 31140 gactcctgtt caacttgtct cacagccaga accttgcctt gtgtgctgtc aactacacgc 31200 gtcaaatagg cattgatctt gaatatctgc gtccaacatc tgacttggag tctcttgcaa 31260 agaggttctt tctcccaaga gaatatgaac tcttgaggtc actccctgat gagcagaaac 31320 agaagatttt ctttcgttac tggacttgca aagaggctta tctcaaagca acgggagatg 31380 gaatagccaa acttgaagag atcgagatag cactcacccc aacagaacct gccaagctcc 31440 aaacagctcc tgcgtggtct ctgttggagc ttgtgccaga tgacaattgt gttgcagctg 31500 tggctgttgc gggttttggt tggcagccca agttctggca ttactgagta gttagcttaa 31560 tcacttaggt caccagtatg aactaaaatg catgtaggtg taagagctca tggagagcat 31620 ggaatattgt atccgaccat gtaacagtat aataactgag ctccatctca cttcttctat 31680 gaataaacaa aggatgttat gatatattaa cactctatct atgcacctta ttgttctatg 31740 ataaatttcc tcttattatt ataaatcatc tgaatcgtga cggcttatgg aatgcttcaa 31800 atagtacaaa aacaaatgtg tactataaga ctttctaaac aattctaact ttagcattgt 31860 gaacgagaca taagtgttaa gaagacataa caattataat ggaagaagtt tgtctccatt 31920 tatatattat atattaccca cttatgtatt atattaggat gttaaggaga cataacaatt 31980 ataaagagag aagtttgtat ccatttatat attatatact acccatttat atattatact 32040 tatccactta tttaatgtct ttataaggtt tgatccatga tatttctaat attttagttg 32100 atatgtatat gaaaaggtac tatttgaact ctcttactct gtataaaggt tggatcatcc 32160 ttaaagtggg tctatttaat tttattgctt cttacagata aaaaaaaaat tatgagttgg 32220 tttgataaaa tattgaagga tttaaaataa taataaataa taaataacat ataatatatg 32280 tatataaatt tattataata taacatttat ctataaaaaa gtaaatattg tcataaatct 32340 atacaatcgt ttagccttgc tggaacgaat ctcaattatt taaacgagag taaacatatt 32400 tgactttttg gttatttaac aaattattat ttaacactat atgaaatttt ttttttttat 32460 cagcaaagaa taaaattaaa ttaagaagga caatggtgtc ccaatcctta tacaaccaac 32520 ttccacaaga aagtcaagtc agagacaaca aaaaaacaag caaaggaaat tttttaattt 32580 gagttgtctt gtttgctgca taatttatgc agtaaaacac tacacataac ccttttagca 32640 gtagagcaat ggttgaccgt gtgcttagct tcttttattt tattttttta tcagcaaaga 32700 ataaataaaa taaaatgaga cacttcaggg atgtttcaac ccttatacaa aaccccaaaa 32760 acaagtttcc tagcacccta ccaacgaatt cgcggccgct taattaacta ctgtcactga 32820 ggccgtagac gagtacggac tgatctaact agtgctagcc tcgaggtcga cacccagctt 32880 tcttgtacaa agtggttgcg gccgcttaat taaatttaaa ttcaattaat gcaatcttga 32940 ttttcaacaa cgaaggtaat ggcgtaaaag aaaaaatgta tgttattgta ttgatctttc 33000 atgatgttga agcgtgccat aatatgatga tgtataatta aaatattaac tgtcgcattt 33060 tattgaaatg gcactgttat ttcaaccata tctttgattc tgttacatga cacgactgca 33120 agaagtaaat aatagacgcc gttgttaaag aattgctatc atatgtgcct aactagaggg 33180 aatttgagcg tcagacctaa tcaaatatta caaaatatct cactctgtcg ccagcaatgg 33240 tgtaatcagc gcagacaaat ggcgtaaaga tcgcggaaaa acctccccga gtggcatgat 33300 agctgcctct gtattgctga tttagtcagc cttatttgac ttaagggtgc cctcgttagt 33360 gacaaattgc tttcaaggag acagccatgc cccacacttt gttgaaaaac aaattgcctt 33420 tggggagacg gtaaagccag ttgctcttca ataaggaatg tcgaggaggc aatgtaaccg 33480 cctctggtag tacacttctc taatccaaaa atcaatttgt attcaagata ccgcaaaaaa 33540 cttatggttt aaaccctgca ggactagtcc agaaggtaat tatccaagat gtagcatcaa 33600 gaatccaatg tttacgggaa aaactatgga agtattatgt aagctcagca agaagcagat 33660 caatatgcgg cacatatgca acctatgttc aaaaatgaag aatgtacaga tacaagatcc 33720 tatactgcca gaatacgaag aagaatacgt agaaattgaa aaagaagaac caggcgaaga 33780 aaagaatctt gaagacgtaa gcactgacga caacaatgaa aagaagaaga taaggtcggt 33840 gattgtgaaa gagacataga ggacacatgt aaggtggaaa atgtaagggc ggaaagtaac 33900 cttatcacaa aggaatctta tcccccacta cttatccttt tatatttttc cgtgtcattt 33960 ttgcccttga gttttcctat ataaggaacc aagttcggca tttgtgaaaa caagaaaaaa 34020 tttggtgtaa gctattttct ttgaagtact gaggatacaa cttcagagaa atttgtaagt 34080 ttgtaggtac cagatctgga tcccaaacca tgtctccgga gaggagacca gttgagatta 34140 ggccagctac agcagctgat atggccgcgg tttgtgatat cgttaaccat tacattgaga 34200 cgtctacagt gaactttagg acagagccac aaacaccaca agagtggatt gatgatctag 34260 agaggttgca agatagatac ccttggttgg ttgctgaggt tgagggtgtt gtggctggta 34320 ttgcttacgc tgggccctgg aaggctagga acgcttacga ttggacagtt gagagtactg 34380 tttacgtgtc acataggcat caaaggttgg gcctaggatc tacattgtac acacatttgc 34440 ttaagtctat ggaggcgcaa ggttttaagt ctgtggttgc tgttataggc cttccaaacg 34500 atccatctgt taggttgcat gaggctttgg gatacacagc ccggggtaca ttgcgcgcag 34560 ctggatacaa gcatggtgga tggcatgatg ttggtttttg gcaaagggat tttgagttgc 34620 cagctcctcc aaggccagtt aggccagtta cccaaatctg agtagttagc ttaatcacct 34680 agagctcgat cggcggcaat agcttcttag cgccatcccg ggttgatcct atctgtgttg 34740 aaatagttgc ggtgggcaag gctctctttc agaaagacag gcggccaaag gaacccaagg 34800 tgaggtgggc tatggctctc agttccttgt ggaagcgctt ggtctaaggt gcagaggtgt 34860 tagcgggatg aagcaaaagt gtccgattgt aacaagatat gttgatccta cgtaaggata 34920 ttaaagtatg tattcatcac taatataatc agtgtattcc aatatgtact acgatttcca 34980 atgtctttat tgtcgccgta tgtaatcggc gtcacaaaat aatccccggt gactttcttt 35040 taatccagga tgaaataata tgttattata atttttgcga tttggtccgt tataggaatt 35100 gaagtgtgct tgaggtcggt cgccaccact cccatttcat aattttacat gtatttgaaa 35160 aataaaaatt tatggtattc aatttaaaca cgtatacttg taaagaatga tatcttgaaa 35220 gaaatatagt ttaaatattt attgataaaa taacaagtca ggtattatag tccaagcaaa 35280 aacataaatt tattgatgca agtttaaatt cagaaatatt tcaataactg attatatcag 35340 ctggtacatt gccgtagatg aaagactgag tgcgatatta tggtgtaata cataggaatt 35400 cgtttaaacg atctgcgtct aattttcggt ccaacttgca caggaaagac gtcgaccgcg 35460 gtagctcttg cccagcagac tgggcttcca gtcctttcgc tcgatcgggt ccaatgttgt 35520 cctcagctgt gaaccggaag cggacgacca acagtggaag aactgaaagg aacgagccgt 35580 ctataccttg atgatcggcc tctggtgaag ggtatcatcg cagccaagca agctcatgaa 35640 aggctgatgg gggaggtgta taattatgag gcccacggcg ggcttattct ttagggagga 35700 tctatctcgt tgctcaagtg catggcgcaa agcagttatt ggagtgcgga ttttcgttgg 35760 catattattc gccacgagtt agcagacgaa gagaccttca tgaacgtggc caaggccaga 35820 gttaagcaga tgttacgccc tgctgcaggc ctttctatta tccaatagtt ggttgatctt 35880 tggaaagagc ctcggctgag gcccatactg aaagagatcg atggatatcg atatgccatg 35940 ttgtttgcta gccagaacca gatcacatcc gatatgctat tgcagcttga cgcagatatg 36000 gaggataagt tgattcatgg gatcgctcag gagtagctca tccatgcacg ccgacaagaa 36060 cagaaattcc gtcgagttaa cgcagccgct tacgacggat tcgaaggtca tccattcgga 36120 atgtattagt ttgcaccagc tccgcgtcac acctgtcttc atttgaataa gatgttagca 36180 attgttttta gctttgtctt gttgtggcag ggcggcaagt gcttcagaca tcattctgtt 36240 ttcaaatttt atgctggaga acagcttctt aattcctttg gaaataatag actgcgtctt 36300 aaaattcaga tgtctggata tagatatgat tgtaaaataa cctatttaag tgtcatttag 36360 aacataagtt ttatgaatgt tcttccattt tcgtcatcga acgaataaga gtaaatacac 36420 cttttttaac attacaaata agttcttata cgttgtttat acaccgggaa tcatttccat 36480 tattttcgcg caaaagtcac ggatattcgt gaaagcgaca taaactgcga aatttgcggg 36540 gagtgtcttg agtttgcctc gaggctagcg catgcacata gacacacaca tcatctcatt 36600 gatgcttggt aataattgtc attagattgt ttttatgcat agatgcactc gaaatcagcc 36660 aattttagac aagtatcaaa cggatgtgac ttcagtacat taaaaacgtc cgcaatgtgt 36720 tattaagttg tctaagcgtc aatttgattt acaattgaat atatcctgcc ccagccagcc 36780 aacagctcga tttacaattg aatatatcct gccggccggc ccacgcgtgt cgaggaattc 36840 tgatctggcc cccatttgga cgtgaatgta gacacgtcga aataaagatt tccgaattag 36900 aataatttgt ttattgcttt cgcctataaa tacgacggat cgtaatttgt cgttttatca 36960 aaatgtactt tcattttata ataacgctgc ggacatctac atttttgaat tgaaaaaaaa 37020 ttggtaatta ctctttcttt ttctccatat tgaccatcat actcattgct gatccatgta 37080 gatttcccgg acatgaagcc atttacaatt gaatatatcc tgccgccgct gccgctttgc 37140 acccggtgga gcttgcatgt tggtttctac gcagaactga gccggttagg cagataattt 37200 ccattgagaa ctgagccatg tgcaccttcc ccccaacacg gtgagcgacg gggcaacgga 37260 gtgatccaca tgggactttt aaacatcatc cgtcggatgg cgttgcgaga gaagcagtcg 37320 atccgtgaga tcagccgacg caccgggcag gcgcgcaaca cgatcgcaaa gtatttgaac 37380 gcaggtacaa tcgagccgac gttcacgcgg aacgaccaag caagcttggc tgccattttt 37440 ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta 37500 gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca 37560 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa 37620 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc 37680 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc 37740 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg 37800 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc 37860 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc 37920 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca 37980 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct 38040 cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc 38100 agcggcgagg gcaaccagcc cggtgagcgt cggaaagggt cgacggatct tttccgctgc 38160 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg 38220 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca 38280 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc 38340 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc 38400 cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca ggaagggcag 38460 cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg aaaaggcggc 38520 ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct acaaaatcac 38580 gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg acctgggccg 38640 cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc ggttcggtga 38700 tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc ttggcaaggt 38760 catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc taaaacggcc 38820 ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag agcgacttcg 38880 cggagctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 38940 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 39000 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 39060 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 39120 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 39180 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 39240 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 39300 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 39360 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 39420 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 39480 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 39540 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 39600 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 39660 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 39720 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 39780 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 39840 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 39900 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 39960 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 40020 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 40080 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 40140 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 40200 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 40260 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 40320 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 40380 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 40440 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 40500 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 40560 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 40620 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 40680 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 40740 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 40800 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 40860 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 40920 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 40980 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 41040 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 41100 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 41160 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 41220 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 41280 ttccttactg ggctttctca gcccccgagc gcttagtggg aatttgtacc ccttatcgaa 41340 ccgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc 41400 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta 41460 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 41520 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 41580 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 41640 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 41700 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 41760 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 41820 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 41880 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 41940 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 42000 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 42060 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga 42120 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa 42180 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 42240 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg 42300 aaggtggttc taagcctcgt cttgcgatgg catttcgatc cattcccatt ccgcgctcaa 42360 gatggcttcc cctcggcagt tcatcagggc taaatcaatc tagccgactt gtccggtgaa 42420 atgggctgca ctccaacaga aacaatcaaa caaacataca cagcgactta ttcacacgag 42480 ctcaaattac aacggtatat atcctgccag tcagcatcat cacaccaaaa gttaggcccg 42540 aatagtttga aattagaaag ctcgcaattg aggtctacag gccaaattcg ctcttagccg 42600 tacaatatta ctcaccggat cctaaccggt ttaattaagg cgcgccatgc ccgggcaagc 42660 ggccgcacaa gttt 42674 <210> 55 <211> 40406 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9396 <400> 55 cttgtacaaa gtggttgcgg ccgcttaatt aaatttaaat tcaattaatg caatcttgat 60 tttcaacaac gaaggtaatg gcgtaaaaga aaaaatgtat gttattgtat tgatctttca 120 tgatgttgaa gcgtgccata atatgatgat gtataattaa aatattaact gtcgcatttt 180 attgaaatgg cactgttatt tcaaccatat ctttgattct gttacatgac acgactgcaa 240 gaagtaaata atagacgccg ttgttaaaga attgctatca tatgtgccta actagaggga 300 atttgagcgt cagacctaat caaatattac aaaatatctc actctgtcgc cagcaatggt 360 gtaatcagcg cagacaaatg gcgtaaagat cgcggaaaaa cctccccgag tggcatgata 420 gctgcctctg tattgctgat ttagtcagcc ttatttgact taagggtgcc ctcgttagtg 480 acaaattgct ttcaaggaga cagccatgcc ccacactttg ttgaaaaaca aattgccttt 540 ggggagacgg taaagccagt tgctcttcaa taaggaatgt cgaggaggca atgtaaccgc 600 ctctggtagt acacttctct aatccaaaaa tcaatttgta ttcaagatac cgcaaaaaac 660 ttatggttta aaccctgcag gactagtcca gaaggtaatt atccaagatg tagcatcaag 720 aatccaatgt ttacgggaaa aactatggaa gtattatgta agctcagcaa gaagcagatc 780 aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat acaagatcct 840 atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc aggcgaagaa 900 aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat aaggtcggtg 960 attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg gaaagtaacc 1020 ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc gtgtcatttt 1080 tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac aagaaaaaat 1140 ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa tttgtaagtt 1200 tgtaggtacc agatctggat cccaaaccat gtctccggag aggagaccag ttgagattag 1260 gccagctaca gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac 1320 gtctacagtg aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga 1380 gaggttgcaa gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat 1440 tgcttacgct gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt 1500 ttacgtgtca cataggcatc aaaggttggg cctaggatct acattgtaca cacatttgct 1560 taagtctatg gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga 1620 tccatctgtt aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc 1680 tggatacaag catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc 1740 agctcctcca aggccagtta ggccagttac ccaaatctga gtagttagct taatcaccta 1800 gagctcgatc ggcggcaata gcttcttagc gccatcccgg gttgatccta tctgtgttga 1860 aatagttgcg gtgggcaagg ctctctttca gaaagacagg cggccaaagg aacccaaggt 1920 gaggtgggct atggctctca gttccttgtg gaagcgcttg gtctaaggtg cagaggtgtt 1980 agcgggatga agcaaaagtg tccgattgta acaagatatg ttgatcctac gtaaggatat 2040 taaagtatgt attcatcact aatataatca gtgtattcca atatgtacta cgatttccaa 2100 tgtctttatt gtcgccgtat gtaatcggcg tcacaaaata atccccggtg actttctttt 2160 aatccaggat gaaataatat gttattataa tttttgcgat ttggtccgtt ataggaattg 2220 aagtgtgctt gaggtcggtc gccaccactc ccatttcata attttacatg tatttgaaaa 2280 ataaaaattt atggtattca atttaaacac gtatacttgt aaagaatgat atcttgaaag 2340 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa 2400 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc 2460 tggtacattg ccgtagatga aagactgagt gcgatattat ggtgtaatac ataggaattc 2520 gtttaaacga tctgcgtcta attttcggtc caacttgcac aggaaagacg tcgaccgcgg 2580 tagctcttgc ccagcagact gggcttccag tcctttcgct cgatcgggtc caatgttgtc 2640 ctcagctgtg aaccggaagc ggacgaccaa cagtggaaga actgaaagga acgagccgtc 2700 tataccttga tgatcggcct ctggtgaagg gtatcatcgc agccaagcaa gctcatgaaa 2760 ggctgatggg ggaggtgtat aattatgagg cccacggcgg gcttattctt tagggaggat 2820 ctatctcgtt gctcaagtgc atggcgcaaa gcagttattg gagtgcggat tttcgttggc 2880 atattattcg ccacgagtta gcagacgaag agaccttcat gaacgtggcc aaggccagag 2940 ttaagcagat gttacgccct gctgcaggcc tttctattat ccaatagttg gttgatcttt 3000 ggaaagagcc tcggctgagg cccatactga aagagatcga tggatatcga tatgccatgt 3060 tgtttgctag ccagaaccag atcacatccg atatgctatt gcagcttgac gcagatatgg 3120 aggataagtt gattcatggg atcgctcagg agtagctcat ccatgcacgc cgacaagaac 3180 agaaattccg tcgagttaac gcagccgctt acgacggatt cgaaggtcat ccattcggaa 3240 tgtattagtt tgcaccagct ccgcgtcaca cctgtcttca tttgaataag atgttagcaa 3300 ttgtttttag ctttgtcttg ttgtggcagg gcggcaagtg cttcagacat cattctgttt 3360 tcaaatttta tgctggagaa cagcttctta attcctttgg aaataataga ctgcgtctta 3420 aaattcagat gtctggatat agatatgatt gtaaaataac ctatttaagt gtcatttaga 3480 acataagttt tatgaatgtt cttccatttt cgtcatcgaa cgaataagag taaatacacc 3540 ttttttaaca ttacaaataa gttcttatac gttgtttata caccgggaat catttccatt 3600 attttcgcgc aaaagtcacg gatattcgtg aaagcgacat aaactgcgaa atttgcgggg 3660 agtgtcttga gtttgcctcg aggctagcgc atgcacatag acacacacat catctcattg 3720 atgcttggta ataattgtca ttagattgtt tttatgcata gatgcactcg aaatcagcca 3780 attttagaca agtatcaaac ggatgtgact tcagtacatt aaaaacgtcc gcaatgtgtt 3840 attaagttgt ctaagcgtca atttgattta caattgaata tatcctgccc cagccagcca 3900 acagctcgat ttacaattga atatatcctg ccggccggcc cacgcgtgtc gaggaattct 3960 gatctggccc ccatttggac gtgaatgtag acacgtcgaa ataaagattt ccgaattaga 4020 ataatttgtt tattgctttc gcctataaat acgacggatc gtaatttgtc gttttatcaa 4080 aatgtacttt cattttataa taacgctgcg gacatctaca tttttgaatt gaaaaaaaat 4140 tggtaattac tctttctttt tctccatatt gaccatcata ctcattgctg atccatgtag 4200 atttcccgga catgaagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4260 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4320 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4380 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4440 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 4500 caggtacaat cgagccgacg ttcacgcgga acgaccaagc aagcttggct gccatttttg 4560 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 4620 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 4680 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 4740 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 4800 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 4860 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 4920 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 4980 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 5040 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 5100 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 5160 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 5220 gcggcgaggg caaccagccc ggtgagcgtc ggaaagggtc gacggatctt ttccgctgca 5280 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 5340 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 5400 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 5460 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc 5520 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc 5580 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg 5640 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg 5700 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc 5760 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat 5820 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc 5880 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg 5940 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc 6000 ggagctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 6060 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 6120 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 6180 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 6240 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 6300 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 6360 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 6420 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 6480 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 6540 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 6600 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 6660 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 6720 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 6780 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 6840 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 6900 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 6960 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 7020 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 7080 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 7140 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 7200 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 7260 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 7320 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 7380 tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 7440 ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 7500 gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc 7560 cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag 7620 aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac 7680 atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct 7740 cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc 7800 ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt 7860 ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc 7920 tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc 7980 gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga 8040 ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca 8100 ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg 8160 gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc 8220 gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg 8280 ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga 8340 gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt 8400 tccttactgg gctttctcag cccccgagcg cttagtggga atttgtaccc cttatcgaac 8460 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct 8520 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 8580 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 8640 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 8700 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 8760 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 8820 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 8880 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 8940 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 9000 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 9060 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 9120 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 9180 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac 9240 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag 9300 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 9360 tcgcggcgcg gcttaactca agcgttagag agctggggaa gactatgcgc gatctgttga 9420 aggtggttct aagcctcgtc ttgcgatggc atttcgatcc attcccattc cgcgctcaag 9480 atggcttccc ctcggcagtt catcagggct aaatcaatct agccgacttg tccggtgaaa 9540 tgggctgcac tccaacagaa acaatcaaac aaacatacac agcgacttat tcacacgagc 9600 tcaaattaca acggtatata tcctgccagt cagcatcatc acaccaaaag ttaggcccga 9660 atagtttgaa attagaaagc tcgcaattga ggtctacagg ccaaattcgc tcttagccgt 9720 acaatattac tcaccggatc ctaaccggtt taattaaggc gcgccatgcc cgggcaagcg 9780 gccgcacaag tttgtacaaa aaagcaggct tctagaccta ggtggagtca tcacgcagac 9840 tatctcagca tgtgcgtagc acgcggccgc ctcccagtat cattatagtg aaagttttgg 9900 ctctctcgcc ggtggttttt tacctctatt taaaggggtt ttccacctaa aaattctggt 9960 atcattctca ctttacttgt tactttaatt tctcataatc tttggttgaa attatcacgc 10020 ttccgcacac gatatcccta caaatttatt atttgttaaa cattttcaaa ccgcataaaa 10080 ttttatgaag tcccgtctat ctttaatgta gtctaacatt ttcatattga aatatataat 10140 ttacttaatt ttagcgttgg tagaaagcat aatgatttat tcttattctt cttcatataa 10200 atgtttaata tacaatataa acaaattctt taccttaaga aggatttccc attttatatt 10260 ttaaaaatat atttatcaaa tatttttcaa ccacgtaaat ctcataataa taagttgttt 10320 caaaagtaat aaaatttaac tccataattt ttttattcga ctgatcttaa agcaacaccc 10380 agtgacacaa ctagccattt ttttctttga ataaaaaaat ccaattatca ttgtattttt 10440 tttatacaat gaaaatttca ccaaacaatg atttgtggta tttctgaagc aagtcatgtt 10500 atgcaaaatt ctataattcc catttgacac tacggaagta actgaagatc tgcttttaca 10560 tgcgagacac atcttctaaa gtaattttaa taatagttac tatattcaag atttcatata 10620 tcaaatactc aatattactt ctaaaaaatt aattagatat aattaaaata ttactttttt 10680 aattttaagt ttaattgttg aatttgtgac tattgattta ttattctact atgtttaaat 10740 tgttttatag atagtttaaa gtaaatataa gtaatgtagt agagtgttag agtgttaccc 10800 taaaccataa actataagat ttatggtgga ctaattttca tatatttctt attgctttta 10860 ccttttcttg gtatgtaagt ccgtaactgg aattactgtg ggttgccatg acactctgtg 10920 gtcttttggt tcatgcatgg atcttgcgca agaaaaagac aaagaacaaa gaaaaaagac 10980 aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa ttagtcactg gctgatcaag 11040 atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa cacgtgctta acatgcactt 11100 taaatggctc acccatctca acccacacac aaacacattg cctttttctt catcatcacc 11160 acaaccacct gtatatattc attctcttcc gccacctcaa tttcttcact tcaacacacg 11220 tcaacctgca tatgcgtgtc atcccatgcc caaatctcca tgcatgttcc aaccaccttc 11280 tctcttatat aatacctata aatacctcta atatcactca cttctttcat catccatcca 11340 tccagagtac tactactcta ctactataat accccaaccc aactcatatt caatactact 11400 ctaggatcca caaccaaaaa tggcagcgag gctgcaagag cagaaaggag gtgagatgga 11460 cacgaggatt gcgatcatag gcatgagcgc catacttcct tgtggaacca cggtgagaga 11520 gtcatgggag accataaggg ctggcattga ttgcctgtct gatcttcctg aggatcgtgt 11580 tgatgtgact gcgtactttg acccagtcaa gaccacaaag gacaaaatct actgcaagag 11640 aggaggtttc attcctgagt atgactttga tgcgcgtgag tttggtctca acatgttcca 11700 gatggaagat tctgatgcga atcagaccat ctcacttctc aaggtcaaag aagcacttca 11760 agatgctggg attgatgcac ttggaaagga gaagaaaaac ataggatgcg tgcttggcat 11820 tggaggtgga cagaagagtt cacatgagtt ctacagcaga ctcaactatg tggttgtgga 11880 gaaagtcttg agaaagatgg ggatgccaga ggaagatgtc aaagtggctg tggagaagta 11940 caaagcgaac ttcccagaat ggagacttga ctctttccct ggattccttg ggaatgtcac 12000 tgctggcaga tgcaccaaca cattcaactt ggatggcatg aactgcgttg tggatgctgc 12060 atgtgcaagc tccttgatag cagtcaaggt tgcgattgat gaactgctct atggtgactg 12120 cgacatgatg gtcactggtg caacctgcac agacaactcc attgggatgt acatggcttt 12180 ctcaaagaca cctgtgttca gcacggaccc aagtgtgaga gcgtatgatg agaaaacgaa 12240 agggatgctc attggtgagg gatcagccat gttggtgctc aaacgttatg cagatgcagt 12300 gagggatggt gatgagattc atgctgtgat tcgtggttgt gcaagctcaa gtgatggcaa 12360 agcagctggc atctacactc ccacaatcag tggtcaagag gaagccttga gacgtgcata 12420 caatcgtgcc tgcgttgacc ctgccactgt cactttggtt gagggtcatg ggactggcac 12480 tccagttgga gataggattg agttgactgc gttgaggaac ttgtttgaca aagcgtatgg 12540 tgaagggaac acagagaaag ttgcagttgg aagtatcaag tccagtattg gtcatttgaa 12600 ggctgttgct ggtcttgctg gaatgatcaa ggtcatcatg gctctcaaac acaagacttt 12660 gcctggaacg atcaatgtgg acaatccacc caatctctat gacaacactc ccatcaatga 12720 gtcatccctc tacatcaaca cgatgaacag accctggttc cctccacctg gtgttccgag 12780 acgtgctgga atatccagct ttggctttgg aggtgccaac taccatgcag tgttggaaga 12840 ggctgaacca gagcacacga cagcgtacag actcaacaaa cgtccacaac cggtgttgat 12900 gatggctgca actcctgctg cgttgcagag cctttgtgaa gctcagctga aagagtttga 12960 agctgcgatc aaggagaatg agacagtcaa gaatactgcc tacatcaagt gtgtcaagtt 13020 tggagaacag ttcaagttcc ctgggagcat acctgcgacc aatgcgagac tgggattctt 13080 ggtcaaagat gcggaggatg cgtgctccac gcttcgtgct atatgtgctc agtttgccaa 13140 agatgtgacc aaagaagcgt ggaggcttcc gagagaagga gtttctttcc gtgccaaagg 13200 cattgccacc aatggtgctg ttgctgcact cttctctgga caaggtgctc agtacacaca 13260 catgttcagt gaagttgcga tgaactggcc tcagttcaga cagtccattg ctgcaatgga 13320 cgctgcacag tccaaggttg ctggaagtga caaagacttt gaaagagtct ctcaagtcct 13380 gtacccaagg aaaccgtacg aacgtgagcc agaacaagat cacaagaaaa tctcactcac 13440 agcatacagc cagccgtcaa ccctggcatg tgctcttggt gcctttgaga ttttcaagga 13500 agctggattc actccagact ttgcagctgg acacagcttg ggtgagtttg ctgcgctcta 13560 tgcagctggt tgtgttgaca gagatgagtt gtttgaactt gtttgcagaa gggcaaggat 13620 catgggaggc aaagatgcac ctgccacacc caaaggttgc atggcagctg tcataggacc 13680 caacgcagag aacatcaagg ttcaagctgc caacgtctgg cttggaaact caaactctcc 13740 gtctcagact gtcattactg ggtctgttga agggattcaa gctgaaagtg ccagattgca 13800 gaaggaaggg ttcagagttg tgcctcttgc ttgtgagtct gccttccatt ctccacagat 13860 ggaaaatgcc agctcagctt tcaaagatgt catatccaag gtttccttcc gtacacccaa 13920 agcagaaaca aagctcttca gcaatgtttc tggagagacc tatccgacgg atgccagaga 13980 gatgctcact caacacatga cctcatctgt gaagttcctg acccaagtga ggaacatgca 14040 ccaagctggt gcgagaatct ttgttgagtt tggaccgaaa caagttctca gcaaacttgt 14100 ttctgaaacc ctcaaagatg acccgtctgt tgtcacagtg tctgtgaacc cagcttctgg 14160 cacggactct gacatacagt tgagggatgc tgcagttcag ctggttgtgg ctggtgtcaa 14220 tcttcaaggg tttgacaagt gggatgctcc tgatgccacg aggatgcaag ccatcaagaa 14280 aaagagaacc acactcagac tttcagctgc cacctatgtc agtgacaaga ccaagaaagt 14340 gagggatgct gccatgaacg acggaagatg tgtcacatac ctcaagggtg ctgcaccact 14400 catcaaggct cctgaacctg tggttgacga agctgccaag agggaggctg aaaggcttca 14460 gaaggagctt caagacgctc agaggcagct tgacgacgcc aaacgtgcag ctgcagaagc 14520 caactccaaa cttgctgctg ccaaggagga agccaagact gctgcagctt cagccaagcc 14580 tgcagttgac actgctgttg tggagaaaca tcgtgccatc ctcaagtcca tgctggctga 14640 gctggatggt tatggaagtg ttgatgcttc ttcccttcag caacagcaac agcaacagac 14700 tgctccggcg cctgttaaag ccgctgcccc tgccgctccc gttgcaagtg ctcctgctcc 14760 cgccgtgtcc aacgagttgc ttgagaaagc tgagacagtt gtgatggagg ttctcgctgc 14820 caagactgga tacgaaacag acatgattga ggcggacatg gagcttgaga ctgagttggg 14880 aattgacagc atcaagaggg ttgaaattct ttctgaagtc caagctatgc ttaacgttga 14940 ggcgaaagat gttgatgctc tctctaggac gagaacagtg ggtgaagttg tcaacgctat 15000 gaaggcagaa atcgctggtt cttccgctcc ggcgccggca gctgccgcac ctgcaccggc 15060 taaagcggct ccagctgcag ctgcaccagc tgtttctaat gagctgctcg aaaaggcaga 15120 aactgttgtg atggaagtcc ttgctgccaa aactggctac gaaactgaca tgatagaatc 15180 agacatggag cttgagacgg agcttggaat tgattccatc aagcgtgtgg aaatcctcag 15240 tgaagtccaa gccatgctca atgtggaagc taaggatgtg gatgctctct caaggaccag 15300 aactgtcggt gaggttgtca atgccatgaa ggctgagata gctggaggct ccgctcctgc 15360 gccggccgct gccgctcctg gtcccgctgc cgcggctcca gctccagctg cagccgctcc 15420 ggccgtctca aacgaactcc ttgaaaaagc tgagaccgtc gtgatggagg tcttggcagc 15480 taaaaccggc tatgagactg acatgattga gtctgacatg gaattggaga cagaacttgg 15540 gatagacagc atcaaacgtg ttgaaatcct cagtgaggtc caagctatgc tgaacgtgga 15600 agcgaaggat gttgatgctc tgtccagaac gaggaccgtt ggtgaggttg tggatgccat 15660 gaaggctgaa attgctggag ggtctgcgcc cgcgccagct gcagccgctc ctgcacccgc 15720 agccgcggca cctgctccag ctgcacctgc acctgccgtt tcttcagaac tcttggagaa 15780 ggctgagacg gttgtgatgg aggttttggc tgcaaagact ggctatgaga ccgacatgat 15840 tgaatctgat atggaattgg agacagagct tgggatcgat tccatcaaaa gagtggagat 15900 cctgagtgaa gtccaagcca tgctcaatgt tgaagctaag gatgttgatg cactttcaag 15960 aacaaggaca gtgggtgagg ttgtggatgc catgaaggcg gagattgctg gagggagcgc 16020 gcctgcgcca gctgccgcgg cacctgcacc ggcagccgca gctccagctc cagctgcacc 16080 agcaccggcc gcgcccgcgc cagctgtttc atccgaactc ctggaaaagg ccgagacggt 16140 tgtgatggag gtgctcgcag ctaagactgg ttacgagacc gacatgattg aaagtgacat 16200 ggagcttgaa accgagttgg gaatagatag catcaaacgt gttgaaatct tgtctgaggt 16260 ccaagctatg ttgaacgtgg aggcaaaaga tgtcgatgcg ctttcaagaa ccagaacagt 16320 cggtgaggtc gtggacgcca tgaaggctga aattgccgga agctctgcta gtgcgccagc 16380 tgcagccgca cctgcacctg ctgccgcggc acctgctcca gctgcagccg ctcctgccgt 16440 cagcaatgag ttgctggaga aagccgaaac agttgtgatg gaagtccttg ctgcaaaaac 16500 tggttacgaa acggacatga ttgagtcaga catggaattg gagaccgaac ttggaataga 16560 ttccatcaag agagtggaga tcctctctga ggtgcaagcc atgctcaacg ttgaggctaa 16620 agatgttgat gcactcagta ggacaagaac tgtgggggaa gttgtcgatg cgatgaaggc 16680 tgagattgct ggaggctctg ctcctgcgcc ggcagctgcc gcacctgcac ccgcagccgc 16740 ggcaccagct gtcagcaacg aattgcttga gaaggctgaa acagttgtca tggaagtgct 16800 ggctgcaaaa accggctatg agactgacat gattgagtct gacatggaac ttgagacgga 16860 attgggcatt gacagcatca agcgtgttga gatcctcagt gaagtccaag caatgctcaa 16920 tgtggaggct aaggatgtgg atgccctctc aaggaccaga acagttggtg aggtcgttga 16980 tgcgatgaag gcggagatcg ctggaagttc cgcgcctgcg ccagctgcag ccgctcctgc 17040 acccgcagcc gcggcaccgg ctccagctgc agccgcaccg gccgtctcct cagaactcct 17100 ggaaaaggct gagacagttg tgatggaagt ccttgctgcg aaaactggct atgagaccga 17160 catgattgaa tctgacatgg aactggagac cgagcttgga attgactcca tcaaacgtgt 17220 tgaaatcctc tctgaggttc aagcgatgtt gaatgtggag gccaaagatg ttgatgctct 17280 ttccagaaca aggacggtgg gagaggtggt tgatgccatg aaggctgaga tagctggggg 17340 ttctgctcct gcgccagctg cagccgctcc tgctcccgct gccgcggcac cagctgtcag 17400 caacgaactc cttgaaaaag ctgaaactgt tgtgatggag gttttggctg caaagactgg 17460 atacgagact gacatgatag agtcagacat ggagttggaa acagagcttg ggattgatag 17520 catcaagcgt gtggaaatcc tttctgaggt tcaagccatg ctgaacgttg aggccaaaga 17580 tgtcgatgct ttgtcaagga ccagaacggt tggagaagtg gtcgatgcca tgaaggctga 17640 gatagctggt ggatcagccc ctgctccggc tgcagccgct cctgcaagtg ccggtgctgc 17700 accagctgtg aagattgatt cagttcatgg tgctgactgt gacgatttgt cactgatgca 17760 cgcaaaggtg gttgacatca gaaggccaga tgaactgatt ctggaaagac cggagaaccg 17820 tccagttctt gtcgttgacg atggctcaga gttgacactt gctcttgtga gggtgttggg 17880 agcctgtgca gttgtcttga cctttgaggg attgcagttg gctcagagag ctggtgcagc 17940 tgccattcgt catgttctgg ctaaggacct ttcagcagaa tcagctgaga aggccatcaa 18000 agaggctgag caaagatttg gagcgttggg aggcttcata agccagcaag cagaaaggtt 18060 tgaaccagct gagatactgg gtttcacact catgtgtgcg aagtttgcca aagcctctct 18120 ctgcactgct gtggctggag gtcgtcctgc cttcatcggt gtggctaggc ttgacggcag 18180 acttggattc acctctcaag gcacttctga cgcactcaag agagcacagc gtggagcgat 18240 ctttggactc tgcaagacaa tagggttgga gtggtcagaa tctgatgtgt tcagccgtgg 18300 tgtggacatt gctcaaggga tgcacccaga agatgctgca gttgccatag ttagggagat 18360 ggcgtgtgct gacatcagaa tcagagaggt tgggattgga gcaaaccagc aaagatgcac 18420 aatcagagct gccaagttgg agactgggaa ccctcagaga cagatagcga aagacgatgt 18480 gcttctggtt tctggaggtg ctcgtgggat cacacctctt tgcatccgtg agatcacgcg 18540 tcagattgct ggaggcaagt acattctgtt gggtcgtagc aaagtttctg cgtctgaacc 18600 agcatggtgt gctggcatca ctgatgagaa ggctgtgcag aaagctgcca cccaagagtt 18660 gaagagggcc ttcagtgctg gtgaaggacc caaacccact ccaagggctg tcacgaaact 18720 tgtgggatct gttcttggtg ctcgtgaagt gagaagctcc attgctgcca ttgaagctct 18780 gggtggcaaa gcgatctaca gctcttgtga tgtgaactct gctgcagatg ttgccaaagc 18840 agttagggat gcagagtccc agcttggtgc cagagttagt ggaatcgttc acgcttctgg 18900 tgtgctccgt gatcgtctca tagagaagaa actccctgac gagtttgatg ctgtctttgg 18960 gaccaaagtc actgggttgg agaatcttct ggctgcagtt gatcgtgcca acctcaaaca 19020 catggttctc ttcagctccc ttgctggttt ccacggcaat gttggtcagt ctgactatgc 19080 gatggcaaac gaagctctca acaagatggg tttggaactt gcgaaggatg tcagtgtcaa 19140 gtccatctgc tttggtccct gggatggtgg gatggtgaca cctcaactca agaaacagtt 19200 tcaagaaatg ggagttcaga tcattcctcg tgaaggaggt gctgatacag ttgcgaggat 19260 tgtgttggga tcaagtcctg ctgagatcct tgttggaaac tggaggacac catccaagaa 19320 agttggaagt gacacgatca ccctgcatag aaagatcagt gccaagtcca atcccttctt 19380 ggaggatcat gtcatacaag gcagaagggt gttgccaatg acccttgcta ttgggagcct 19440 tgctgagacc tgccttggac tcttccctgg ctactctctc tgggccatag atgacgcaca 19500 gctcttcaaa ggtgtcactg ttgatggaga cgtcaactgt gaagtgaccc tcacaccgtc 19560 cactgctccg agtgggaggg tgaatgtcca agcgacgctc aagacgttct caagtggaaa 19620 acttgttcca gcctacagag ctgtcatagt gttgtccaac caaggtgctc cacctgccaa 19680 tgcaaccatg cagcctccga gccttgatgc agatcctgct ttgcaagggt cagtctatga 19740 tggcaagact ctctttcatg gtcctgcgtt cagaggcatt gatgacgttc tcagctgcac 19800 caagagccag cttgttgcca agtgttctgc tgttcctgga tcagatgcag ccagaggaga 19860 gtttgcaact gacactgatg cacatgatcc ctttgtcaat gatcttgcct ttcaagcaat 19920 gttggtctgg gtgaggagaa cgctgggaca agctgcactc ccgaactcca ttcagaggat 19980 agtccaacac agaccggtcc cacaagacaa acccttctac atcaccctca gatccaatca 20040 gagtggtgga catagccagc acaaacatgc acttcagttc cacaacgaac aaggtgatct 20100 cttcattgat gttcaagcct ctgtcattgc gacagactca cttgcgttct gagtagttag 20160 cttaatcact taggtcacca gtatgaacta aaatgcatgt aggtgtaaga gctcatggag 20220 agcatggaat attgtatccg accatgtaac agtataataa ctgagctcca tctcacttct 20280 tctatgaata aacaaaggat gttatgatat attaacactc tatctatgca ccttattgtt 20340 ctatgataaa tttcctctta ttattataaa tcatctgaat cgtgacggct tatggaatgc 20400 ttcaaatagt acaaaaacaa atgtgtacta taagactttc taaacaattc taactttagc 20460 attgtgaacg agacataagt gttaagaaga cataacaatt ataatggaag aagtttgtct 20520 ccatttatat attatatatt acccacttat gtattatatt aggatgttaa ggagacataa 20580 caattataaa gagagaagtt tgtatccatt tatatattat atactaccca tttatatatt 20640 atacttatcc acttatttaa tgtctttata aggtttgatc catgatattt ctaatatttt 20700 agttgatatg tatatgaaaa ggtactattt gaactctctt actctgtata aaggttggat 20760 catccttaaa gtgggtctat ttaattttat tgcttcttac agataaaaaa aaaattatga 20820 gttggtttga taaaatattg aaggatttaa aataataata aataataaat aacatataat 20880 atatgtatat aaatttatta taatataaca tttatctata aaaaagtaaa tattgtcata 20940 aatctataca atcgtttagc cttgctggaa cgaatctcaa ttatttaaac gagagtaaac 21000 atatttgact ttttggttat ttaacaaatt attatttaac actatatgaa attttttttt 21060 tttatcagca aagaataaaa ttaaattaag aaggacaatg gtgtcccaat ccttatacaa 21120 ccaacttcca caagaaagtc aagtcagaga caacaaaaaa acaagcaaag gaaatttttt 21180 aatttgagtt gtcttgtttg ctgcataatt tatgcagtaa aacactacac ataacccttt 21240 tagcagtaga gcaatggttg accgtgtgct tagcttcttt tattttattt ttttatcagc 21300 aaagaataaa taaaataaaa tgagacactt cagggatgtt tcaaccctta tacaaaaccc 21360 caaaaacaag tttcctagca ccctaccaac gaattcgcgg ccgcttaatt aagatgagtg 21420 atactcagga ctcaggactc actctgctga tcactagtgc tagcctcgag gtcgacacaa 21480 ctttgtatac aaaagttgtc tagacctagg ctcagcaact catccagtca agttcgagtc 21540 agtgagttgg gcggccgcag cttatagtaa agattttttt gttaataaaa tgaaagttaa 21600 tatttatgga tttatttgaa catatttaca agtttttgtt aataaattta tttacagaag 21660 tgttaaattt gaaaatgtgt taaatgagag ttaatattta tggatataat attactttat 21720 ttaaaaataa aatgaattta tattaaaata tgatttggta aaagatatta taaagaggaa 21780 cttggatgag aaagacgacg atgggcaaaa gttgcatggc acaccgcttt tgttcagtaa 21840 catctgcact gtggcgcctt tttcacactc accatgcatg ctgccacgtc agctttcaca 21900 ctatgacacc actggccata cacgttgcca cctcagcttt ctcctcttct cactatgaca 21960 cgactggcca tgcatgcatg catgctgcca cctcagctcc cgcctcttca cccgtgtctt 22020 tctctcatgt cgcagtgctg cgtgccaacc tcttctctct ataaatagag agaagagaat 22080 gatggttaaa ctcaaaccaa aacaagaaaa catacacaaa tagcaaaacg gatccacaac 22140 caaaaatggc tgcaaggaac gtgagtgcgg ctcatgagat gcatgacgag aagaggatag 22200 ctgttgtggg catggctgtt cagtatgctg gatgcaaaac gaaagacgag ttctgggaag 22260 ttctcatgaa tggaaaagtt gagagcaaag tgatcagcga caaacgtctt ggttcaaatt 22320 accgtgctga acactacaaa gctgaaagga gcaagtatgc tgacaccttc tgcaatgaga 22380 catatggaac tcttgatgag aacgaaatag acaatgagca tgaactcctt ttgaaccttg 22440 ccaaacaagc cttggctgaa acatctgtca aagattcaac aagatgtggc atagtttctg 22500 gatgcctcag cttcccgatg gacaacctcc aaggtgaact cttgaatgtg taccagaacc 22560 atgttgagaa gaaacttggt gccagagttt tcaaagatgc ctctcactgg tcagaaagag 22620 aacagtccaa caaaccagag gctggtgaca gaagaatctt catggaccct gcttcctttg 22680 ttgctgagga actcaatctt ggcgctctcc actactctgt tgatgctgcc tgtgccactg 22740 cgctctatgt gctcagactg gcgcaagatc acttggtctc tggtgcagct gatgtgatgc 22800 tttgtggtgc aacatgcctg ccggagccgt ttttcatact ctctgggttc tcaaccttcc 22860 aagccatgcc tgttggaacg ggtcagaatg tgtccatgcc gctgcacaaa gattctcaag 22920 gattgacccc tggtgaagga ggttccatca tggtcctcaa acgtttggat gacgcaatca 22980 gagatggaga tcacatctat ggaacacttt tgggagccaa tgtcagcaac agtggcactg 23040 gtctgccact taaacccctt ttgccgtcag agaaaaagtg cctcatggac acctacacac 23100 gtatcaatgt gcatccccac aagattcagt atgttgaatg ccatgcaact gggactccac 23160 aaggtgatcg tgtggaaatt gatgctgtga aggcttgctt tgaaggcaaa gtcccacgtt 23220 ttggaaccac aaaagggaac tttggacaca cattggtggc agctgggttt gctggcatgt 23280 gcaaagtgct tctctccatg aagcacggga tcattccacc cactccggga atagatgacg 23340 aaacgaagat ggaccctctg gttgtctctg gtgaggccat tccctggcca gaaacgaatg 23400 gagaaccgaa gcgtgctggt ctctcagcgt ttggatttgg tgggaccaat gctcatgctg 23460 tctttgagga acatgaccca agcaatgctg catgcactgg ccatgactcc atttctgctc 23520 tctctgcacg ttgtggtggt gagtcaaaca tgagaatagc aatcactggg atggatgcca 23580 cttttggtgc tttgaaagga ttggatgctt ttgaaagggc catctacact ggtgctcatg 23640 gtgctatacc actcccagaa aagagatgga gattcctggg gaaagacaag gacttccttg 23700 atctttgtgg agtcaaagca acaccgcatg gctgttacat tgaagatgtt gaagttgatt 23760 tccagaggct gaggactcca atgactcctg aagatatgct cttgcctcaa cagcttctgg 23820 ctgtcaccac aattgatagg gccatattgg actctggcat gaagaaaggt ggaaatgtgg 23880 ctgtctttgt ggggttgggg accgatcttg aactctaccg tcaccgtgca agggttgctc 23940 tcaaggagag agtgaggcct gaagccagca agaaactcaa tgacatgatg cagtacatca 24000 atgattgtgg gacaagcact tcttacacca gctacatagg caacttggtt gcaacaaggg 24060 tcagttcaca atggggcttc acgggacctt ccttcacaat cacagaaggc aacaattctg 24120 tgtacagatg tgctgaactt ggcaaatacc tcttggaaac gggagaggtt gatggggttg 24180 tggttgcggg tgttgatctc tgtgggagtg ctgagaacct ttacgtcaag tcaaggcgtt 24240 tcaaggtgtc tacatctgac accccgagag ccagctttga tgcagctgct gatggttact 24300 ttgttggaga gggctgtggt gcatttgtgc tcaagcgtga gacaagctgc accaaggatg 24360 acagaatcta tgcttgcatg gatgccatag ttcctggaaa tgttccatct gcatgtttga 24420 gagaggcatt ggatcaagcc agagtcaagc ctggagacat cgagatgttg gagctttctg 24480 ctgattcagc gaggcatttg aaggacccgt ctgttctccc aaaggaactc actgctgagg 24540 aagagattgg cggacttcag acgattcttc gtgacgatga caagctccca aggaatgttg 24600 caactggaag tgtcaaggcc actgttggtg acactggtta tgcttctggt gctgccagtt 24660 tgattaaggc tgcgctttgc atctacaacc gttacctgcc cagcaatgga gatgactggg 24720 atgaacctgc acctgaagca ccctgggaca gcacactctt tgcgtgccag acctcaagag 24780 cttggttgaa gaaccctggt gagagacgtt atgcggctgt ttccggagtc agtgaaacca 24840 gaagttgcta cagtgtgctg ctttctgaag cagagggaca ctacgagaga gagaacagaa 24900 tcagtttgga tgaggaagca cccaagctca ttgtgttgag agcagactcc catgaagaga 24960 ttcttggaag attggacaag ataagggaaa ggttcttgca gcccacgggt gctgcaccca 25020 gagagtctga attgaaggcg caagcccgta ggatctttct tgaactcctt ggtgagaccc 25080 ttgcccaaga tgcagcttca tctggaagtc agaagcccct tgctctcagc ttggtctcca 25140 ctccatccaa gctccagaga gaggtggaac ttgcggcaaa aggcattccg agatgtctca 25200 agatgagacg tgattggagt tcccctgctg gaagcagata tgcgcctgag cctttggcca 25260 gtgacagagt tgccttcatg tatggagaag gaaggagccc ttactatggc atcacacaag 25320 acattcatag gatttggcct gaacttcatg aggtcatcaa tgagaaaaca aaccgtctct 25380 gggctgaagg tgacagatgg gtcatgccaa gggccagctt caagagtgag cttgagtcac 25440 aacagcaaga gtttgacaga aacatgattg aaatgttccg tcttggaatc ctcacctcaa 25500 ttgccttcac aaacctggcg agagatgttc tcaacatcac gcccaaagca gcctttggac 25560 tcagtttggg agagatttcc atgatctttg ctttctccaa gaaaaacggt ttgataagcg 25620 atcagttgac aaaggatttg agagagtcag atgtctggaa caaagctctg gctgttgagt 25680 tcaatgcgct cagagaagcc tggggcattc cacagtctgt cccaaaagat gagttctggc 25740 aaggttacat agtgagggga acgaaacaag acatagaagc agccatagca ccagactcaa 25800 agtatgtgag gttgaccatc atcaatgatg ccaacactgc tctcatcagt ggaaagcctg 25860 atgcatgcaa agctgcgatt gctcgtcttg gtggcaacat ccctgcgctt ccagtcactc 25920 aaggcatgtg tgggcattgc ccagaggtgg gaccttacac caaggacatc gcaaagatcc 25980 acgccaacct tgagttccct gtggttgatg gtcttgatct ctggactacc ataaaccaga 26040 agaggcttgt gccaagagcc acgggtgcca aagatgaatg ggctccttca tcctttgggg 26100 agtatgctgg acagctctat gagaaacaag ccaacttccc tcagatagtt gaaaccatct 26160 acaagcagaa ctatgatgtg tttgttgaag ttgggccaaa caatcacaga agcacagcag 26220 tgagaaccac gcttggacca cagagaaacc accttgctgg tgccatagac aagcagaacg 26280 aggatgcttg gacgacaata gtcaagctcg tggcttcatt gaaggctcac cttgttcctg 26340 gagtgaccat cagtccgctc taccactcca agctcgttgc agaggctgag gcttgttatg 26400 ctgcgctctg taaaggtgag aaacccaaga aaaacaagtt tgtgagaaag attcagttga 26460 atggcagatt caacagcaag gcagacccca tctcctcagc agaccttgca tccttcccac 26520 ctgcggaccc tgcgattgaa gctgccatct cttcaaggat catgaagcct gtggctccga 26580 aattctatgc gcgtctcaac attgatgagc aagatgagac cagagatccg atcttgaaca 26640 aggacaatgc gccttccagc tccagctctt ccagcagttc ctcttccagc agttcatctc 26700 cgtctcctgc tccttcagct ccagttcaga agaaagctgc cccagctgcg gagacaaaag 26760 ctgttgccag tgctgatgca cttcgtagtg ccctgcttga tcttgactcc atgcttgcgc 26820 tcagctctgc aagtgcttct ggcaaccttg ttgagactgc gccatctgat gccagtgtca 26880 ttgtccctcc gtgcaacatt gctgatcttg gcagcagagc tttcatgaaa acttatggtg 26940 tttctgcgcc tctgtacacc ggtgcaatgg ccaagggaat tgcctctgcg gacttggtga 27000 tcgcagctgg aaggcaagga atccttgcgt catttggtgc tggaggcctt ccaatgcaag 27060 ttgtgcgtga gtccattgaa aagattcaag ctgccctgcc caatggacca tatgctgtca 27120 acctcatcca ttctcctttt gacagcaacc ttgagaaggg aaatgttgat ctcttcttgg 27180 aaaagggtgt cacttttgtt gaggcttcag ccttcatgac gttgacaccg caagttgtca 27240 gatacagagc ggctgggttg acgaggaatg cagatgggtc agtcaacata aggaacagaa 27300 tcattgggaa agtctccaga actgagctgg ctgaaatgtt catgcgtcct gcacctgagc 27360 atcttttgca gaagctcatt gcttctgggg agatcaatca agaacaagct gagttggcca 27420 gacgtgttcc tgtggctgat gacattgcgg ttgaagctga ttctggtgga cacactgaca 27480 accgtccaat ccatgtcatt ctgcctctga ttatcaacct gagggatagg cttcacagag 27540 agtgtggata cccagcaaac ctgagggttc gtgttggtgc tgggggtggc attgggtgtc 27600 cccaagctgc gctggcaact ttcaacatgg gtgcctcttt cattgtcact ggaacagtga 27660 accaagttgc caaacaatct gggacgtgtg acaatgtgag gaaacagctt gcgaaagcaa 27720 cttactcaga tgtttgcatg gcaccggcag ctgacatgtt tgaggaaggt gtgaaacttc 27780 aagttttgaa gaaaggaaca atgttccctt caagagccaa caaactctat gagttgttct 27840 gcaagtatga ttcttttgag tccatgcctc cagcagagct tgcgagggtt gagaaaagaa 27900 tcttcagtcg tgcattggaa gaggtctggg atgagaccaa gaacttctac atcaaccgtc 27960 tccacaaccc agagaagata cagagggctg agagggaccc caaactgaag atgtcactgt 28020 gtttcagatg gtatctgtcc ctggcttcaa ggtgggccaa cactggagct tctgacagag 28080 tcatggacta ccaagtttgg tgtggtcctg ccattggttc attcaatgat ttcatcaaag 28140 gaacttacct tgatccggca gtggcaaatg agtacccgtg tgttgtgcag atcaacaaac 28200 agattcttag aggagcgtgc ttcttgaggc gtcttgaaat actgagaaat gcaaggcttt 28260 cagatggtgc agctgcactt gtggcgagca ttgatgacac atatgtccct gctgagaagc 28320 tgtgagtagt tagcttaatc acttaggtca cccagtccaa gcccggccta gcacgatgat 28380 catgagtgtt gctattgatg tatgttaata ctatacacag tcatcgtgtg tgttttataa 28440 aactgtaagc ttaggatgtt tgaggctaat gtaattagca ctacaccgta ataaatgaga 28500 ggtttacttt atgttttaat aatgttctta cccttgctgc tctctaaact gtaatggcgt 28560 acggttagct aaactaaagt aaaaatcgat tcgcttgagt aaaattagtg accgggaaag 28620 atcatcgact actactgctc agagatgtaa tcggtaatta agacttcaag ctcaataatt 28680 aattaggagt aaatctggct tcgaattttt aaattgtcaa cttccaaaga aggattggca 28740 agactcaatc tcaattgccc aacaaacata tggctcttta caaatttgga atctcttttt 28800 gatggcaaga acgtcgtaat tcctctgtcg atctcatgta attaccaaac tcatctactt 28860 attactttct ttatatgcac ttactgatgc ggccgcttaa ttaatctgac ctgaccacta 28920 gacctgagtg agtacagatg tgctactagt gctagcctcg aggtcgacca acttttctat 28980 acaaagttgt ctagacctag gactaggcac tgagtgactc actgacctga ctgagtgact 29040 ggcggccgca gcttatagta aagatttttt tgttaataaa atgaaagtta atatttatgg 29100 atttatttga acatatttac aagtttttgt taataaattt atttacagaa gtgttaaatt 29160 tgaaaatgtg ttaaatgaga gttaatattt atggatataa tattacttta tttaaaaata 29220 aaatgaattt atattaaaat atgatttggt aaaagatatt ataaagagga acttggatga 29280 gaaagacgac gatgggcaaa agttgcatgg cacaccgctt ttgttcagta acatctgcac 29340 tgtggcgcct ttttcacact caccatgcat gctgccacgt cagctttcac actatgacac 29400 cactggccat acacgttgcc acctcagctt tctcctcttc tcactatgac acgactggcc 29460 atgcatgcat gcatgctgcc acctcagctc ccgcctcttc acccgtgtct ttctctcatg 29520 tcgcagtgct gcgtgccaac ctcttctctc tataaataga gagaagagaa tgatggttaa 29580 actcaaacca aaacaagaaa acatacacaa atagcaaaac ggatccacaa ccaaaaatgg 29640 cgcttcgtgt caagaccaac aagaagccat gttgggagat gaccaaggag gaactgacct 29700 ctggcaaaac tgaggtgttc aactatgagg aactcctgga gtttgcagag ggtgacatag 29760 ctaaggtttt tggtccggag tttgctgtca tagacaagta ccccagaagg gtgaggctgc 29820 ctgctcgtga atacctgctt gtgacaaggg tcacactcat ggatgccgag gtcaacaact 29880 acagagttgg tgccagaatg gtcactgagt atgatcttcc agtgaatggc gaattgtctg 29940 agggaggtga ttgtccctgg gcagttctgg ttgagagtgg acagtgcgat ttgatgctga 30000 tatcatacat gggaattgat ttccagaacc aaggtgacag agtttaccgt ctgctcaaca 30060 ccacgttgac attctatggg gttgcccatg agggagagac ccttgagtat gacatcagag 30120 tgaccggttt tgccaaacgt ttggatggtg gcatctccat gttcttcttt gagtatgact 30180 gctatgtcaa tgggaggctt ctcattgaga tgagagatgg ctgtgctggc ttcttcacaa 30240 atgaggagct tgatgcggga aagggtgtgg tgttcacaag aggggatttg gctgccagag 30300 ctaagattcc aaagcaagat gtttctccct atgctgtcgc tccctgcctt cacaaaacaa 30360 aactcaacga aaaggagatg cagaccttgg ttgacaaaga ttgggcttcc gtttttggct 30420 caaagaatgg catgccggag atcaactaca aactctgcgc acgtaagatg ctcatgattg 30480 atagagtcac cagcattgac cataagggag gtgtgtacgg tttgggtcag ttggttggtg 30540 agaaaatctt ggagagggac cattggtact tcccatgcca ttttgttaag gatcaagtca 30600 tggctggatc tcttgtcagc gacggttgtt cacaaatgct caagatgtac atgatctggc 30660 tcggtttgca ccttaccacc ggaccatttg acttcagacc cgtcaacgga cacccgaaca 30720 aagttaggtg taggggtcag atatctcctc ataagggaaa gttggtctac gtcatggaga 30780 tcaaagagat gggattcgac gaggataacg atccgtacgc cattgctgac gtcaacatca 30840 ttgacgttga tttcgaaaag ggtcaagatt tcagccttga tagaatcagc gactacggaa 30900 agggggatct taacaaaaag atcgtcgtgg acttcaaggg cattgctctc aagatgcaga 30960 agagatccac gaacaagaac ccctccaaag ttcagcccgt ttttgctaac ggtgcagcca 31020 ctgtgggacc ggaagcgtcc aaggccagct ctggtgccag tgctagtgct tctgctgccc 31080 ctgcgaagcc tgctttctca gcagatgttc ttgcgccaaa gccagttgca cttcctgaac 31140 atatcctcaa gggtgacgct ctcgctccga aagaaatgtc ctggcatccc atgtcaaaac 31200 ttcctggcaa cccgacgccg ttcttctccc ctagttctta ccctccgagg gcaatttgct 31260 tcatcccttt ccctggcaac ccacttgaca acaattgcaa agctggagaa atgcctctga 31320 actggtacaa catgtcagag ttcatgtgtg gaaaagttag caactgcttg ggaccagagt 31380 tcgcaagatt tgataagtca aatacgagca gaagccctgc ttttgatttg gctcttgtga 31440 ctagagttgt tgaagtcaca aacatggaac atggcaagtt cctgaatgtt gattgcaatc 31500 ccagcaaagg cacaatggtg ggggagtttg actgtcctca agatgcgtgg ttctttgatg 31560 gttcttgcaa tgatgggcac atgccttatt ccatcatcat ggaaatcgga cttcagacat 31620 ccggtgttct caccagtgtg ttgaaagcac cactgacgat ggacaaagat gacattctct 31680 ttaggaacct tgatgcaagt gctgaaatgg tgaggccaga tgtggatgtg agggggaaaa 31740 ccataaggaa tgtgaccaag tgcactggat atgcaatgtt gggaaagatg gggattcaca 31800 gattcacgtt tgagttgtct gttgatggcg tggttttcta caaaggaagc acttcctttg 31860 gatggttcac tccagaggtg tttgctcagc aagctggact tgacaatggg aaaaagacgg 31920 agccttggtg caaaactaac aacacgagtg tgagaagagt tgaaatcgca tcagccaaag 31980 gcaaagaaca gctgactgag aaacttccag atgcaactaa tgctcaagtt ctcagaaggt 32040 cagaacagtg tgagtatttg gattacctca acattgctcc tgattctggg ctgcatggga 32100 aaggatatgc gcatggacac aaagatgtta acccacaaga ctggttcttc tcttgtcact 32160 tttggtttga tcctgtgatg cctggatctt tgggaattga gtcaatgttc cagctcattg 32220 aggcatttgc ggtggaccag aacataccgg gagaatacaa tgtgagcaat ccgacctttg 32280 cccatgcccc tggaaaaacg gcgtggaagt accgtggtca attgacacca aagaaccgtg 32340 cgatggactg tgaggtgcac atagtttcaa tcacagcctc tcctgaaaat ggaggctacg 32400 ttgacatagt ggcagatgga gcactttggg ttgatggtct ccgtgtgtat gaagccaaag 32460 aactcagagt tcgtgttgtc agtgcaaaac ctcaagccat tccggatgtt caacaacagc 32520 cacccagcgc aaaagcggac cctgggaaaa ctggagttgc actcagtccg actcagttga 32580 gagatgtcct gcttgaagtg gacaatccat tgtatcttgg tgttgaaaac tccaatttgg 32640 tgcagtttga atccaaacct gcaacttctt caaggattgt cagtatcaaa ccatgctcca 32700 taagtgatct tggtgacaag tccttcatgg aaacgtacaa tgtgtcagca cctctgtaca 32760 ctggagcaat ggccaaggga attgcgtctg cggaccttgt cattgctgct gggaagagga 32820 agatattggg ttcttttgga gcgggtgggc ttccaatgca ccatgtgagg gcagctttgg 32880 agaagataca agcggctctg cctcaaggac catatgctgt gaacctcatt cattcacctt 32940 ttgacagcaa cttggaaaag gggaatgttg atctcttcct cgaaaagggc gttactgtgg 33000 tggaggcttc tgcattcatg accctcactc cccaagttgt tcgttaccgt gcagccggac 33060 tctcaaggaa cgctgatggg agtgtcaaca tcagaaaccg tatcattgga aaagtgtcca 33120 gaacagaact cgccgaaatg ttcatcagac cggctccgga acacctcctt gagaaactca 33180 tagcttctgg tgagataaca caagaacaag ccgaacttgc ccgtcgtgtt cctgtcgcag 33240 acgacatcgc tgttgaagct gattctggtg gtcacactga caaccgtccc atccacgtca 33300 tcttgccttt gatcatcaac cttaggaaca gactgcacag agagtgtggt tatcctgcgc 33360 accttagagt cagagttgga gctggaggcg gtgttggctg cccacaagct gctgcggctg 33420 ccctcacaat gggagctgcc ttcatagtga ctgggactgt caatcaagtt gctaaacagt 33480 ccgggacatg tgacaacgtg agaaaacaac tcagtcaagc cacctacagt gacatttgca 33540 tggctccagc agctgatatg tttgaggaag gtgtgaaact ccaagttctc aagaagggaa 33600 caatgttccc atcaagagct aacaaattgt acgaactctt ttgcaagtac gactctttcg 33660 attcaatgcc tcctgctgaa cttgaaagga tcgagaagag gatcttcaag agggcacttc 33720 aagaggtctg ggaagagacc aaggactttt acatcaatgg tttgaagaat ccagagaaga 33780 tacagagagc ggaacatgac cccaaactca agatgtcttt gtgtttcaga tggtatcttg 33840 gtcttgcatc acgttgggcc aacatgggtg ctcctgatcg tgtcatggac tatcaagtct 33900 ggtgtggtcc agccattgga gccttcaatg acttcatcaa aggaacatat cttgatcctg 33960 ctgtctctaa tgagtaccca tgtgttgtcc agatcaacct tcagattttg cgtggtgcat 34020 gttacctgag gcgtcttaat gctctgagaa atgatccgag gattgatctt gagactgaag 34080 atgctgcctt tgtctatgag ccaacgaatg cgctttgagt agttagctta atcacttagg 34140 tcacccagtc caagcccggc ctagcacgat gatcatgagt gttgctattg atgtatgtta 34200 atactataca cagtcatcgt gtgtgtttta taaaactgta agcttaggat gtttgaggct 34260 aatgtaatta gcactacacc gtaataaatg agaggtttac tttatgtttt aataatgttc 34320 ttacccttgc tgctctctaa actgtaatgg cgtacggtta gctaaactaa agtaaaaatc 34380 gattcgcttg agtaaaatta gtgaccggga aagatcatcg actactactg ctcagagatg 34440 taatcggtaa ttaagacttc aagctcaata attaattagg agtaaatctg gcttcgaatt 34500 tttaaattgt caacttccaa agaaggattg gcaagactca atctcaattg cccaacaaac 34560 atatggctct ttacaaattt ggaatctctt tttgatggca agaacgtcgt aattcctctg 34620 tcgatctcat gtaattacca aactcatcta cttattactt tctttatatg cacttactga 34680 tgcggccgct taattaatca cgagtggatg actgtcacga ctgactcatg actgactact 34740 agtgctagcc tcgaggtcga cacaactttg tataataaag ttgtctagac ctaggcgagt 34800 catgagtcgt gacatgacgt catgctcatc agactgcggc cgcagcttat agtaaagatt 34860 tttttgttaa taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt 34920 ttgttaataa atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat 34980 atttatggat ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt 35040 tggtaaaaga tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc 35100 atggcacacc gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat 35160 gcatgctgcc acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca 35220 gctttctcct cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca 35280 gctcccgcct cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc 35340 tctctataaa tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac 35400 acaaatagca aaacggatcc acaaccaaaa atgcccactc cagatgccac tgcgcctctc 35460 aacaagccgt cagactatgc tgtgtaccac gaagaggatg gtcctttctg gactgctgat 35520 tcctctggtg tcagcagagt gaacttctct gaaactggtg tgggatctga aggtgtcatt 35580 cctgcgctca cactgattga tgtgtttgag cgtgcggtca aacgtggtgg caataggatt 35640 gcattcagaa cagagaacat gccaaccttg aggcgtggag aagaggctcc tgatgctttg 35700 ccgctcaaag attggaagtc ctggtcttgg aaacagtaca aagctgatgt tcacagaatt 35760 gcgagggctc tgatggattt gggagttgag cagcacgatg cggtctccat ctttggcttc 35820 aactcaccag aatggttcct cagtgctgtc ggtgcagttc atgctggtgc caagatcgct 35880 ggaatctacc cttcagacac tcctgctcaa gtgcagtaca aagccttcca ctctgacact 35940 gctgttgcag ttgtggagaa cgaacagtgc ttcaagaaat tcgcagaggt ggttgaggat 36000 ctcccttacc tcaaagccat tgtttgttgg gattatgaag ccacagacat cacaagggaa 36060 gatggttcag tggttgaagt cctgaccttt gctgagttcc tgaagagagg tgacactgtg 36120 gaagcagctg cacttgatga gagaatcagc aagattgagc ccaccatgtg tgctgccctg 36180 atctacacca gtggcacaac tgggagaccc aaagctgtca tgatctccca tgacaacctt 36240 gtctttgaag catctgctgt ggtcccgaac ttgggagggg catgcacgac cactgctgag 36300 gaaaggattc tttcctacct tcctttgtct catgttgctg gaatgatggt tgacatcatt 36360 gctccaatca tagcgacagc gttccacaaa ggaaggatct gtgtttgttt tgccagaccg 36420 tatgatttga gaactggaac ccttgggcaa agactcaatg cagtggagcc aaccatattc 36480 cttggtgtgc cacgtgtgtg ggaaaagatc caagagaaat tgatggctgt tggtgccaaa 36540 accactggtc tcaagaagaa actcagcacg gcagccaaga aacgtgggct tgaatttcaa 36600 gaggaacaac agatagggag aagtggtgcc aaccctggct ttggaccgct tggaatctac 36660 aagaaacttt tgggcctcat caaaggaaag ttggggctga ccaaatgcaa gtttgccttt 36720 gctggcgcag ctccaatgac ccgtgaaacc cttcagtact ttggagcgct gaacatcaac 36780 atcaatgagg tctatggaat gtctgaatgc agtggggctg ccacctggtc aactgacaaa 36840 gctcatgagt ggggcactgt tggctatgag atgccctcct gtgaagtcag agtgttcaag 36900 attgcggagg atggcaccaa gacagagtgc ccaagagctg ctgacatcat gcatgccaca 36960 gaagaggaac aaggtgaagt ttgcttccgt ggaaggaaca tcatgatggg atacttggcc 37020 aacccgaaac ttggtgatga ccatgttgct gagattgaag agaagaatgc agctgccata 37080 gatagcgaag ggtggcttca ctctggtgac aagggagcaa tttccacaag gggaatgctc 37140 aagataacgg gcagatacaa ggagcttatc ataggtgctg gtggggagaa tgtggctcct 37200 gttcccattg aagatgccat caaggcgagg atgccttttg tttcaaatgc aatgatggtt 37260 ggagacaaga ggaagttcat ggctgtcctt ctgactttga aaacggttgg agcaactgga 37320 gagcttcctg gcacaaacaa gctcatggga gcagctgcgg actatggtga gaccattgag 37380 gatgcctgtg acaatgaggc gctcattgaa gagataacgc agcaactcaa agagataggt 37440 gacgatggag atgtcacgcc aagcaatgca gcccgtatac aaaagttcac gattttgcca 37500 ctcgacttca gtgtctccac agatgaactg acagcaacgt tgaaattgaa gagaagtgtg 37560 gttgcagaca agtatgagga catcatagag gctttctatg agagcaagtc tgtctttgtg 37620 ccttactcaa cagttggagc ttatgcaacg ggtggaccag ttgatgactc tgttgtggat 37680 ggatcattca aaggtgactt tagcatgatt ggggatgacg atccggatct tcagaatgtg 37740 gatgtccttg aagcaattga tgaggacaac tgagtagtta gcttaatcac ttaggtcacc 37800 cagtccaagc ccggcctagc acgatgatca tgagtgttgc tattgatgta tgttaatact 37860 atacacagtc atcgtgtgtg ttttataaaa ctgtaagctt aggatgtttg aggctaatgt 37920 aattagcact acaccgtaat aaatgagagg tttactttat gttttaataa tgttcttacc 37980 cttgctgctc tctaaactgt aatggcgtac ggttagctaa actaaagtaa aaatcgattc 38040 gcttgagtaa aattagtgac cgggaaagat catcgactac tactgctcag agatgtaatc 38100 ggtaattaag acttcaagct caataattaa ttaggagtaa atctggcttc gaatttttaa 38160 attgtcaact tccaaagaag gattggcaag actcaatctc aattgcccaa caaacatatg 38220 gctctttaca aatttggaat ctctttttga tggcaagaac gtcgtaattc ctctgtcgat 38280 ctcatgtaat taccaaactc atctacttat tactttcttt atatgcactt actgatgcgg 38340 ccgcttaatt aatctgatca tggatgctac gtcacggcag tacaggacta tcactagacc 38400 taggagctgt gctgggtaac tcacatgagt cactggtcac atcagcggcc gcagcttata 38460 gtaaagattt ttttgttaat aaaatgaaag ttaatattta tggatttatt tgaacatatt 38520 tacaagtttt tgttaataaa tttatttaca gaagtgttaa atttgaaaat gtgttaaatg 38580 agagttaata tttatggata taatattact ttatttaaaa ataaaatgaa tttatattaa 38640 aatatgattt ggtaaaagat attataaaga ggaacttgga tgagaaagac gacgatgggc 38700 aaaagttgca tggcacaccg cttttgttca gtaacatctg cactgtggcg cctttttcac 38760 actcaccatg catgctgcca cgtcagcttt cacactatga caccactggc catacacgtt 38820 gccacctcag ctttctcctc ttctcactat gacacgactg gccatgcatg catgcatgct 38880 gccacctcag ctcccgcctc ttcacccgtg tctttctctc atgtcgcagt gctgcgtgcc 38940 aacctcttct ctctataaat agagagaaga gaatgatggt taaactcaaa ccaaaacaag 39000 aaaacataca caaatagcaa aacggatcca caaccaaaaa tgcttcagca cacttggctt 39060 ccgaagcctc ccaatctgac cctcttgtca gatgaggttc atctctggag gattcctctt 39120 gaccagcctg agtcacaact tcaagacctt gctgccaccc tgagcagtga tgaattggcg 39180 agggcaaaca gattctactt tccagaacac agaaggcgtt tcactgctgg gagaggcatc 39240 ctcagatcca tcttgggtgg atacttggga gtggaaccgg gtcaagtcaa gtttgattat 39300 gagtcccgtg ggaaaccgat ccttggtgac agatttgctg agagtggact cctgttcaac 39360 ttgtctcaca gccagaacct tgccttgtgt gctgtcaact acacgcgtca aataggcatt 39420 gatcttgaat atctgcgtcc aacatctgac ttggagtctc ttgcaaagag gttctttctc 39480 ccaagagaat atgaactctt gaggtcactc cctgatgagc agaaacagaa gattttcttt 39540 cgttactgga cttgcaaaga ggcttatctc aaagcaacgg gagatggaat agccaaactt 39600 gaagagatcg agatagcact caccccaaca gaacctgcca agctccaaac agctcctgcg 39660 tggtctctgt tggagcttgt gccagatgac aattgtgttg cagctgtggc tgttgcgggt 39720 tttggttggc agcccaagtt ctggcattac tgagtagtta gcttaatcac ttaggtcacc 39780 cagtccaagc ccggcctagc acgatgatca tgagtgttgc tattgatgta tgttaatact 39840 atacacagtc atcgtgtgtg ttttataaaa ctgtaagctt aggatgtttg aggctaatgt 39900 aattagcact acaccgtaat aaatgagagg tttactttat gttttaataa tgttcttacc 39960 cttgctgctc tctaaactgt aatggcgtac ggttagctaa actaaagtaa aaatcgattc 40020 gcttgagtaa aattagtgac cgggaaagat catcgactac tactgctcag agatgtaatc 40080 ggtaattaag acttcaagct caataattaa ttaggagtaa atctggcttc gaatttttaa 40140 attgtcaact tccaaagaag gattggcaag actcaatctc aattgcccaa caaacatatg 40200 gctctttaca aatttggaat ctctttttga tggcaagaac gtcgtaattc ctctgtcgat 40260 ctcatgtaat taccaaactc atctacttat tactttcttt atatgcactt actgatgcgg 40320 ccgcttaatt aactactgtc actgaggccg tagacgagta cggactgatc taactagtgc 40380 tagcctcgag gtcgacaccc agcttt 40406 <210> 56 <211> 39630 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB101412 <400> 56 cttgtacaaa gtggttgcgg ccgcttaatt aaatttaaat tcaattaatg caatcttgat 60 tttcaacaac gaaggtaatg gcgtaaaaga aaaaatgtat gttattgtat tgatctttca 120 tgatgttgaa gcgtgccata atatgatgat gtataattaa aatattaact gtcgcatttt 180 attgaaatgg cactgttatt tcaaccatat ctttgattct gttacatgac acgactgcaa 240 gaagtaaata atagacgccg ttgttaaaga attgctatca tatgtgccta actagaggga 300 atttgagcgt cagacctaat caaatattac aaaatatctc actctgtcgc cagcaatggt 360 gtaatcagcg cagacaaatg gcgtaaagat cgcggaaaaa cctccccgag tggcatgata 420 gctgcctctg tattgctgat ttagtcagcc ttatttgact taagggtgcc ctcgttagtg 480 acaaattgct ttcaaggaga cagccatgcc ccacactttg ttgaaaaaca aattgccttt 540 ggggagacgg taaagccagt tgctcttcaa taaggaatgt cgaggaggca atgtaaccgc 600 ctctggtagt acacttctct aatccaaaaa tcaatttgta ttcaagatac cgcaaaaaac 660 ttatggttta aaccctgcag gactagtcca gaaggtaatt atccaagatg tagcatcaag 720 aatccaatgt ttacgggaaa aactatggaa gtattatgta agctcagcaa gaagcagatc 780 aatatgcggc acatatgcaa cctatgttca aaaatgaaga atgtacagat acaagatcct 840 atactgccag aatacgaaga agaatacgta gaaattgaaa aagaagaacc aggcgaagaa 900 aagaatcttg aagacgtaag cactgacgac aacaatgaaa agaagaagat aaggtcggtg 960 attgtgaaag agacatagag gacacatgta aggtggaaaa tgtaagggcg gaaagtaacc 1020 ttatcacaaa ggaatcttat cccccactac ttatcctttt atatttttcc gtgtcatttt 1080 tgcccttgag ttttcctata taaggaacca agttcggcat ttgtgaaaac aagaaaaaat 1140 ttggtgtaag ctattttctt tgaagtactg aggatacaac ttcagagaaa tttgtaagtt 1200 tgtaggtacc agatctggat cccaaaccat gtctccggag aggagaccag ttgagattag 1260 gccagctaca gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac 1320 gtctacagtg aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga 1380 gaggttgcaa gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat 1440 tgcttacgct gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt 1500 ttacgtgtca cataggcatc aaaggttggg cctaggatct acattgtaca cacatttgct 1560 taagtctatg gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga 1620 tccatctgtt aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc 1680 tggatacaag catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc 1740 agctcctcca aggccagtta ggccagttac ccaaatctga gtagttagct taatcaccta 1800 gagctcgatc ggcggcaata gcttcttagc gccatcccgg gttgatccta tctgtgttga 1860 aatagttgcg gtgggcaagg ctctctttca gaaagacagg cggccaaagg aacccaaggt 1920 gaggtgggct atggctctca gttccttgtg gaagcgcttg gtctaaggtg cagaggtgtt 1980 agcgggatga agcaaaagtg tccgattgta acaagatatg ttgatcctac gtaaggatat 2040 taaagtatgt attcatcact aatataatca gtgtattcca atatgtacta cgatttccaa 2100 tgtctttatt gtcgccgtat gtaatcggcg tcacaaaata atccccggtg actttctttt 2160 aatccaggat gaaataatat gttattataa tttttgcgat ttggtccgtt ataggaattg 2220 aagtgtgctt gaggtcggtc gccaccactc ccatttcata attttacatg tatttgaaaa 2280 ataaaaattt atggtattca atttaaacac gtatacttgt aaagaatgat atcttgaaag 2340 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa 2400 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc 2460 tggtacattg ccgtagatga aagactgagt gcgatattat ggtgtaatac ataggaattc 2520 gtttaaacga tctgcgtcta attttcggtc caacttgcac aggaaagacg tcgaccgcgg 2580 tagctcttgc ccagcagact gggcttccag tcctttcgct cgatcgggtc caatgttgtc 2640 ctcagctgtg aaccggaagc ggacgaccaa cagtggaaga actgaaagga acgagccgtc 2700 tataccttga tgatcggcct ctggtgaagg gtatcatcgc agccaagcaa gctcatgaaa 2760 ggctgatggg ggaggtgtat aattatgagg cccacggcgg gcttattctt tagggaggat 2820 ctatctcgtt gctcaagtgc atggcgcaaa gcagttattg gagtgcggat tttcgttggc 2880 atattattcg ccacgagtta gcagacgaag agaccttcat gaacgtggcc aaggccagag 2940 ttaagcagat gttacgccct gctgcaggcc tttctattat ccaatagttg gttgatcttt 3000 ggaaagagcc tcggctgagg cccatactga aagagatcga tggatatcga tatgccatgt 3060 tgtttgctag ccagaaccag atcacatccg atatgctatt gcagcttgac gcagatatgg 3120 aggataagtt gattcatggg atcgctcagg agtagctcat ccatgcacgc cgacaagaac 3180 agaaattccg tcgagttaac gcagccgctt acgacggatt cgaaggtcat ccattcggaa 3240 tgtattagtt tgcaccagct ccgcgtcaca cctgtcttca tttgaataag atgttagcaa 3300 ttgtttttag ctttgtcttg ttgtggcagg gcggcaagtg cttcagacat cattctgttt 3360 tcaaatttta tgctggagaa cagcttctta attcctttgg aaataataga ctgcgtctta 3420 aaattcagat gtctggatat agatatgatt gtaaaataac ctatttaagt gtcatttaga 3480 acataagttt tatgaatgtt cttccatttt cgtcatcgaa cgaataagag taaatacacc 3540 ttttttaaca ttacaaataa gttcttatac gttgtttata caccgggaat catttccatt 3600 attttcgcgc aaaagtcacg gatattcgtg aaagcgacat aaactgcgaa atttgcgggg 3660 agtgtcttga gtttgcctcg aggctagcgc atgcacatag acacacacat catctcattg 3720 atgcttggta ataattgtca ttagattgtt tttatgcata gatgcactcg aaatcagcca 3780 attttagaca agtatcaaac ggatgtgact tcagtacatt aaaaacgtcc gcaatgtgtt 3840 attaagttgt ctaagcgtca atttgattta caattgaata tatcctgccc cagccagcca 3900 acagctcgat ttacaattga atatatcctg ccggccggcc cacgcgtgtc gaggaattct 3960 gatctggccc ccatttggac gtgaatgtag acacgtcgaa ataaagattt ccgaattaga 4020 ataatttgtt tattgctttc gcctataaat acgacggatc gtaatttgtc gttttatcaa 4080 aatgtacttt cattttataa taacgctgcg gacatctaca tttttgaatt gaaaaaaaat 4140 tggtaattac tctttctttt tctccatatt gaccatcata ctcattgctg atccatgtag 4200 atttcccgga catgaagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4260 cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4320 cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4380 tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4440 tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 4500 caggtacaat cgagccgacg ttcacgcgga acgaccaagc aagcttggct gccatttttg 4560 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 4620 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 4680 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 4740 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 4800 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 4860 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 4920 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 4980 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 5040 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 5100 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 5160 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 5220 gcggcgaggg caaccagccc ggtgagcgtc ggaaagggtc gacggatctt ttccgctgca 5280 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 5340 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 5400 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 5460 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc 5520 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc 5580 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg 5640 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg 5700 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc 5760 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat 5820 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc 5880 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg 5940 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc 6000 ggagctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 6060 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 6120 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 6180 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 6240 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 6300 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 6360 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 6420 tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 6480 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 6540 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 6600 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 6660 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 6720 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 6780 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 6840 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 6900 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 6960 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 7020 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 7080 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 7140 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 7200 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 7260 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 7320 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 7380 tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 7440 ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 7500 gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc 7560 cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag 7620 aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac 7680 atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct 7740 cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc 7800 ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt 7860 ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc 7920 tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc 7980 gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga 8040 ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca 8100 ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg 8160 gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc 8220 gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg 8280 ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga 8340 gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt 8400 tccttactgg gctttctcag cccccgagcg cttagtggga atttgtaccc cttatcgaac 8460 cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct 8520 taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 8580 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 8640 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 8700 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 8760 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 8820 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 8880 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 8940 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 9000 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 9060 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 9120 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 9180 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac 9240 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag 9300 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct 9360 tcgcggcgcg gcttaactca agcgttagag agctggggaa gactatgcgc gatctgttga 9420 aggtggttct aagcctcgtc ttgcgatggc atttcgatcc attcccattc cgcgctcaag 9480 atggcttccc ctcggcagtt catcagggct aaatcaatct agccgacttg tccggtgaaa 9540 tgggctgcac tccaacagaa acaatcaaac aaacatacac agcgacttat tcacacgagc 9600 tcaaattaca acggtatata tcctgccagt cagcatcatc acaccaaaag ttaggcccga 9660 atagtttgaa attagaaagc tcgcaattga ggtctacagg ccaaattcgc tcttagccgt 9720 acaatattac tcaccggatc ctaaccggtt taattaaggc gcgccatgcc cgggcaagcg 9780 gccgcacaag tttgtacaaa aaagcaggct tctaggatct gcttttacat gcgagacaca 9840 tcttctaaag taattttaat aatagttact atattcaaga tttcatatat caaatactca 9900 atattacttc taaaaaatta attagatata attaaaatat tactttttta attttaagtt 9960 taattgttga atttgtgact attgatttat tattctacta tgtttaaatt gttttataga 10020 tagtttaaag taaatataag taatgtagta gagtgttaga gtgttaccct aaaccataaa 10080 ctataagatt tatggtggac taattttcat atatttctta ttgcttttac cttttcttgg 10140 tatgtaagtc cgtaactgga attactgtgg gttgccatga cactctgtgg tcttttggtt 10200 catgcatgga tcttgcgcaa gaaaaagaca aagaacaaag aaaaaagaca aaacagagag 10260 acaaaacgca atcacacaac caactcaaat tagtcactgg ctgatcaaga tcgccgcgtc 10320 catgtatgtc taaatgccat gcaaagcaac acgtgcttaa catgcacttt aaatggctca 10380 cccatctcaa cccacacaca aacacattgc ctttttcttc atcatcacca caaccacctg 10440 tatatattca ttctcttccg ccacctcaat ttcttcactt caacacacgt caacctgcat 10500 atgcgtgtca tcccatgccc aaatctccat gcatgttcca accaccttct ctcttatata 10560 atacctataa atacctctaa tatcactcac ttctttcatc atccatccat ccagagtact 10620 actactctac tactataata ccccaaccca actcatattc aatactactc taggatccac 10680 aaccaaaaat ggcagcgagg ctgcaagagc agaaaggagg tgagatggac acgaggattg 10740 cgatcatagg catgagcgcc atacttcctt gtggaaccac ggtgagagag tcatgggaga 10800 ccataagggc tggcattgat tgcctgtctg atcttcctga ggatcgtgtt gatgtgactg 10860 cgtactttga cccagtcaag accacaaagg acaaaatcta ctgcaagaga ggaggtttca 10920 ttcctgagta tgactttgat gcgcgtgagt ttggtctcaa catgttccag atggaagatt 10980 ctgatgcgaa tcagaccatc tcacttctca aggtcaaaga agcacttcaa gatgctggga 11040 ttgatgcact tggaaaggag aagaaaaaca taggatgcgt gcttggcatt ggaggtggac 11100 agaagagttc acatgagttc tacagcagac tcaactatgt ggttgtggag aaagtcttga 11160 gaaagatggg gatgccagag gaagatgtca aagtggctgt ggagaagtac aaagcgaact 11220 tcccagaatg gagacttgac tctttccctg gattccttgg gaatgtcact gctggcagat 11280 gcaccaacac attcaacttg gatggcatga actgcgttgt ggatgctgca tgtgcaagct 11340 ccttgatagc agtcaaggtt gcgattgatg aactgctcta tggtgactgc gacatgatgg 11400 tcactggtgc aacctgcaca gacaactcca ttgggatgta catggctttc tcaaagacac 11460 ctgtgttcag cacggaccca agtgtgagag cgtatgatga gaaaacgaaa gggatgctca 11520 ttggtgaggg atcagccatg ttggtgctca aacgttatgc agatgcagtg agggatggtg 11580 atgagattca tgctgtgatt cgtggttgtg caagctcaag tgatggcaaa gcagctggca 11640 tctacactcc cacaatcagt ggtcaagagg aagccttgag acgtgcatac aatcgtgcct 11700 gcgttgaccc tgccactgtc actttggttg agggtcatgg gactggcact ccagttggag 11760 ataggattga gttgactgcg ttgaggaact tgtttgacaa agcgtatggt gaagggaaca 11820 cagagaaagt tgcagttgga agtatcaagt ccagtattgg tcatttgaag gctgttgctg 11880 gtcttgctgg aatgatcaag gtcatcatgg ctctcaaaca caagactttg cctggaacga 11940 tcaatgtgga caatccaccc aatctctatg acaacactcc catcaatgag tcatccctct 12000 acatcaacac gatgaacaga ccctggttcc ctccacctgg tgttccgaga cgtgctggaa 12060 tatccagctt tggctttgga ggtgccaact accatgcagt gttggaagag gctgaaccag 12120 agcacacgac agcgtacaga ctcaacaaac gtccacaacc ggtgttgatg atggctgcaa 12180 ctcctgctgc gttgcagagc ctttgtgaag ctcagctgaa agagtttgaa gctgcgatca 12240 aggagaatga gacagtcaag aatactgcct acatcaagtg tgtcaagttt ggagaacagt 12300 tcaagttccc tgggagcata cctgcgacca atgcgagact gggattcttg gtcaaagatg 12360 cggaggatgc gtgctccacg cttcgtgcta tatgtgctca gtttgccaaa gatgtgacca 12420 aagaagcgtg gaggcttccg agagaaggag tttctttccg tgccaaaggc attgccacca 12480 atggtgctgt tgctgcactc ttctctggac aaggtgctca gtacacacac atgttcagtg 12540 aagttgcgat gaactggcct cagttcagac agtccattgc tgcaatggac gctgcacagt 12600 ccaaggttgc tggaagtgac aaagactttg aaagagtctc tcaagtcctg tacccaagga 12660 aaccgtacga acgtgagcca gaacaagatc acaagaaaat ctcactcaca gcatacagcc 12720 agccgtcaac cctggcatgt gctcttggtg cctttgagat tttcaaggaa gctggattca 12780 ctccagactt tgcagctgga cacagcttgg gtgagtttgc tgcgctctat gcagctggtt 12840 gtgttgacag agatgagttg tttgaacttg tttgcagaag ggcaaggatc atgggaggca 12900 aagatgcacc tgccacaccc aaaggttgca tggcagctgt cataggaccc aacgcagaga 12960 acatcaaggt tcaagctgcc aacgtctggc ttggaaactc aaactctccg tctcagactg 13020 tcattactgg gtctgttgaa gggattcaag ctgaaagtgc cagattgcag aaggaagggt 13080 tcagagttgt gcctcttgct tgtgagtctg ccttccattc tccacagatg gaaaatgcca 13140 gctcagcttt caaagatgtc atatccaagg tttccttccg tacacccaaa gcagaaacaa 13200 agctcttcag caatgtttct ggagagacct atccgacgga tgccagagag atgctcactc 13260 aacacatgac ctcatctgtg aagttcctga cccaagtgag gaacatgcac caagctggtg 13320 cgagaatctt tgttgagttt ggaccgaaac aagttctcag caaacttgtt tctgaaaccc 13380 tcaaagatga cccgtctgtt gtcacagtgt ctgtgaaccc agcttctggc acggactctg 13440 acatacagtt gagggatgct gcagttcagc tggttgtggc tggtgtcaat cttcaagggt 13500 ttgacaagtg ggatgctcct gatgccacga ggatgcaagc catcaagaaa aagagaacca 13560 cactcagact ttcagctgcc acctatgtca gtgacaagac caagaaagtg agggatgctg 13620 ccatgaacga cggaagatgt gtcacatacc tcaagggtgc tgcaccactc atcaaggctc 13680 ctgaacctgt ggttgacgaa gctgccaaga gggaggctga aaggcttcag aaggagcttc 13740 aagacgctca gaggcagctt gacgacgcca aacgtgcagc tgcagaagcc aactccaaac 13800 ttgctgctgc caaggaggaa gccaagactg ctgcagcttc agccaagcct gcagttgaca 13860 ctgctgttgt ggagaaacat cgtgccatcc tcaagtccat gctggctgag ctggatggtt 13920 atggaagtgt tgatgcttct tcccttcagc aacagcaaca gcaacagact gctccggcgc 13980 ctgttaaagc cgctgcccct gccgctcccg ttgcaagtgc tcctgctccc gccgtgtcca 14040 acgagttgct tgagaaagct gagacagttg tgatggaggt tctcgctgcc aagactggat 14100 acgaaacaga catgattgag gcggacatgg agcttgagac tgagttggga attgacagca 14160 tcaagagggt tgaaattctt tctgaagtcc aagctatgct taacgttgag gcgaaagatg 14220 ttgatgctct ctctaggacg agaacagtgg gtgaagttgt caacgctatg aaggcagaaa 14280 tcgctggttc ttccgctccg gcgccggcag ctgccgcacc tgcaccggct aaagcggctc 14340 cagctgcagc tgcaccagct gtttctaatg agctgctcga aaaggcagaa actgttgtga 14400 tggaagtcct tgctgccaaa actggctacg aaactgacat gatagaatca gacatggagc 14460 ttgagacgga gcttggaatt gattccatca agcgtgtgga aatcctcagt gaagtccaag 14520 ccatgctcaa tgtggaagct aaggatgtgg atgctctctc aaggaccaga actgtcggtg 14580 aggttgtcaa tgccatgaag gctgagatag ctggaggctc cgctcctgcg ccggccgctg 14640 ccgctcctgg tcccgctgcc gcggctccag ctccagctgc agccgctccg gccgtctcaa 14700 acgaactcct tgaaaaagct gagaccgtcg tgatggaggt cttggcagct aaaaccggct 14760 atgagactga catgattgag tctgacatgg aattggagac agaacttggg atagacagca 14820 tcaaacgtgt tgaaatcctc agtgaggtcc aagctatgct gaacgtggaa gcgaaggatg 14880 ttgatgctct gtccagaacg aggaccgttg gtgaggttgt ggatgccatg aaggctgaaa 14940 ttgctggagg gtctgcgccc gcgccagctg cagccgctcc tgcacccgca gccgcggcac 15000 ctgctccagc tgcacctgca cctgccgttt cttcagaact cttggagaag gctgagacgg 15060 ttgtgatgga ggttttggct gcaaagactg gctatgagac cgacatgatt gaatctgata 15120 tggaattgga gacagagctt gggatcgatt ccatcaaaag agtggagatc ctgagtgaag 15180 tccaagccat gctcaatgtt gaagctaagg atgttgatgc actttcaaga acaaggacag 15240 tgggtgaggt tgtggatgcc atgaaggcgg agattgctgg agggagcgcg cctgcgccag 15300 ctgccgcggc acctgcaccg gcagccgcag ctccagctcc agctgcacca gcaccggccg 15360 cgcccgcgcc agctgtttca tccgaactcc tggaaaaggc cgagacggtt gtgatggagg 15420 tgctcgcagc taagactggt tacgagaccg acatgattga aagtgacatg gagcttgaaa 15480 ccgagttggg aatagatagc atcaaacgtg ttgaaatctt gtctgaggtc caagctatgt 15540 tgaacgtgga ggcaaaagat gtcgatgcgc tttcaagaac cagaacagtc ggtgaggtcg 15600 tggacgccat gaaggctgaa attgccggaa gctctgctag tgcgccagct gcagccgcac 15660 ctgcacctgc tgccgcggca cctgctccag ctgcagccgc tcctgccgtc agcaatgagt 15720 tgctggagaa agccgaaaca gttgtgatgg aagtccttgc tgcaaaaact ggttacgaaa 15780 cggacatgat tgagtcagac atggaattgg agaccgaact tggaatagat tccatcaaga 15840 gagtggagat cctctctgag gtgcaagcca tgctcaacgt tgaggctaaa gatgttgatg 15900 cactcagtag gacaagaact gtgggggaag ttgtcgatgc gatgaaggct gagattgctg 15960 gaggctctgc tcctgcgccg gcagctgccg cacctgcacc cgcagccgcg gcaccagctg 16020 tcagcaacga attgcttgag aaggctgaaa cagttgtcat ggaagtgctg gctgcaaaaa 16080 ccggctatga gactgacatg attgagtctg acatggaact tgagacggaa ttgggcattg 16140 acagcatcaa gcgtgttgag atcctcagtg aagtccaagc aatgctcaat gtggaggcta 16200 aggatgtgga tgccctctca aggaccagaa cagttggtga ggtcgttgat gcgatgaagg 16260 cggagatcgc tggaagttcc gcgcctgcgc cagctgcagc cgctcctgca cccgcagccg 16320 cggcaccggc tccagctgca gccgcaccgg ccgtctcctc agaactcctg gaaaaggctg 16380 agacagttgt gatggaagtc cttgctgcga aaactggcta tgagaccgac atgattgaat 16440 ctgacatgga actggagacc gagcttggaa ttgactccat caaacgtgtt gaaatcctct 16500 ctgaggttca agcgatgttg aatgtggagg ccaaagatgt tgatgctctt tccagaacaa 16560 ggacggtggg agaggtggtt gatgccatga aggctgagat agctgggggt tctgctcctg 16620 cgccagctgc agccgctcct gctcccgctg ccgcggcacc agctgtcagc aacgaactcc 16680 ttgaaaaagc tgaaactgtt gtgatggagg ttttggctgc aaagactgga tacgagactg 16740 acatgataga gtcagacatg gagttggaaa cagagcttgg gattgatagc atcaagcgtg 16800 tggaaatcct ttctgaggtt caagccatgc tgaacgttga ggccaaagat gtcgatgctt 16860 tgtcaaggac cagaacggtt ggagaagtgg tcgatgccat gaaggctgag atagctggtg 16920 gatcagcccc tgctccggct gcagccgctc ctgcaagtgc cggtgctgca ccagctgtga 16980 agattgattc agttcatggt gctgactgtg acgatttgtc actgatgcac gcaaaggtgg 17040 ttgacatcag aaggccagat gaactgattc tggaaagacc ggagaaccgt ccagttcttg 17100 tcgttgacga tggctcagag ttgacacttg ctcttgtgag ggtgttggga gcctgtgcag 17160 ttgtcttgac ctttgaggga ttgcagttgg ctcagagagc tggtgcagct gccattcgtc 17220 atgttctggc taaggacctt tcagcagaat cagctgagaa ggccatcaaa gaggctgagc 17280 aaagatttgg agcgttggga ggcttcataa gccagcaagc agaaaggttt gaaccagctg 17340 agatactggg tttcacactc atgtgtgcga agtttgccaa agcctctctc tgcactgctg 17400 tggctggagg tcgtcctgcc ttcatcggtg tggctaggct tgacggcaga cttggattca 17460 cctctcaagg cacttctgac gcactcaaga gagcacagcg tggagcgatc tttggactct 17520 gcaagacaat agggttggag tggtcagaat ctgatgtgtt cagccgtggt gtggacattg 17580 ctcaagggat gcacccagaa gatgctgcag ttgccatagt tagggagatg gcgtgtgctg 17640 acatcagaat cagagaggtt gggattggag caaaccagca aagatgcaca atcagagctg 17700 ccaagttgga gactgggaac cctcagagac agatagcgaa agacgatgtg cttctggttt 17760 ctggaggtgc tcgtgggatc acacctcttt gcatccgtga gatcacgcgt cagattgctg 17820 gaggcaagta cattctgttg ggtcgtagca aagtttctgc gtctgaacca gcatggtgtg 17880 ctggcatcac tgatgagaag gctgtgcaga aagctgccac ccaagagttg aagagggcct 17940 tcagtgctgg tgaaggaccc aaacccactc caagggctgt cacgaaactt gtgggatctg 18000 ttcttggtgc tcgtgaagtg agaagctcca ttgctgccat tgaagctctg ggtggcaaag 18060 cgatctacag ctcttgtgat gtgaactctg ctgcagatgt tgccaaagca gttagggatg 18120 cagagtccca gcttggtgcc agagttagtg gaatcgttca cgcttctggt gtgctccgtg 18180 atcgtctcat agagaagaaa ctccctgacg agtttgatgc tgtctttggg accaaagtca 18240 ctgggttgga gaatcttctg gctgcagttg atcgtgccaa cctcaaacac atggttctct 18300 tcagctccct tgctggtttc cacggcaatg ttggtcagtc tgactatgcg atggcaaacg 18360 aagctctcaa caagatgggt ttggaacttg cgaaggatgt cagtgtcaag tccatctgct 18420 ttggtccctg ggatggtggg atggtgacac ctcaactcaa gaaacagttt caagaaatgg 18480 gagttcagat cattcctcgt gaaggaggtg ctgatacagt tgcgaggatt gtgttgggat 18540 caagtcctgc tgagatcctt gttggaaact ggaggacacc atccaagaaa gttggaagtg 18600 acacgatcac cctgcataga aagatcagtg ccaagtccaa tcccttcttg gaggatcatg 18660 tcatacaagg cagaagggtg ttgccaatga cccttgctat tgggagcctt gctgagacct 18720 gccttggact cttccctggc tactctctct gggccataga tgacgcacag ctcttcaaag 18780 gtgtcactgt tgatggagac gtcaactgtg aagtgaccct cacaccgtcc actgctccga 18840 gtgggagggt gaatgtccaa gcgacgctca agacgttctc aagtggaaaa cttgttccag 18900 cctacagagc tgtcatagtg ttgtccaacc aaggtgctcc acctgccaat gcaaccatgc 18960 agcctccgag ccttgatgca gatcctgctt tgcaagggtc agtctatgat ggcaagactc 19020 tctttcatgg tcctgcgttc agaggcattg atgacgttct cagctgcacc aagagccagc 19080 ttgttgccaa gtgttctgct gttcctggat cagatgcagc cagaggagag tttgcaactg 19140 acactgatgc acatgatccc tttgtcaatg atcttgcctt tcaagcaatg ttggtctggg 19200 tgaggagaac gctgggacaa gctgcactcc cgaactccat tcagaggata gtccaacaca 19260 gaccggtccc acaagacaaa cccttctaca tcaccctcag atccaatcag agtggtggac 19320 atagccagca caaacatgca cttcagttcc acaacgaaca aggtgatctc ttcattgatg 19380 ttcaagcctc tgtcattgcg acagactcac ttgcgttctg agtagttagc ttaatcactt 19440 aggtcacctc gagtatcaaa atctatttag aaatacacaa tattttgttg caggcttgct 19500 ggagaatcga tctgctatca taaaaattac aaaaaaattt tatttgcctc aattatttta 19560 ggattggtat taaggacgct taaattattt gtcgggtcac tacgcatcat tgtgattgag 19620 aagatcagcg atacgaaata ttcgtagtac tatcgataat ttatttgaaa attcataaga 19680 aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt 19740 aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat 19800 gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg 19860 atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaaggtt ggcgcgccga 19920 attcgcggcc gcttaattaa gatgagtgat actcaggact caggactcac tctgctgatc 19980 actagtgcta gcctcgaggt cgacacaact ttgtatacaa aagttgtcta ggatctgctt 20040 ttacatgcga gacacatctt ctaaagtaat tttaataata gttactatat tcaagatttc 20100 atatatcaaa tactcaatat tacttctaaa aaattaatta gatataatta aaatattact 20160 tttttaattt taagtttaat tgttgaattt gtgactattg atttattatt ctactatgtt 20220 taaattgttt tatagatagt ttaaagtaaa tataagtaat gtagtagagt gttagagtgt 20280 taccctaaac cataaactat aagatttatg gtggactaat tttcatatat ttcttattgc 20340 ttttaccttt tcttggtatg taagtccgta actggaatta ctgtgggttg ccatgacact 20400 ctgtggtctt ttggttcatg catggatctt gcgcaagaaa aagacaaaga acaaagaaaa 20460 aagacaaaac agagagacaa aacgcaatca cacaaccaac tcaaattagt cactggctga 20520 tcaagatcgc cgcgtccatg tatgtctaaa tgccatgcaa agcaacacgt gcttaacatg 20580 cactttaaat ggctcaccca tctcaaccca cacacaaaca cattgccttt ttcttcatca 20640 tcaccacaac cacctgtata tattcattct cttccgccac ctcaatttct tcacttcaac 20700 acacgtcaac ctgcatatgc gtgtcatccc atgcccaaat ctccatgcat gttccaacca 20760 ccttctctct tatataatac ctataaatac ctctaatatc actcacttct ttcatcatcc 20820 atccatccag agtactacta ctctactact ataatacccc aacccaactc atattcaata 20880 ctactctagg atccacaacc aaaaatggct gcaaggaacg tgagtgcggc tcatgagatg 20940 catgacgaga agaggatagc tgttgtgggc atggctgttc agtatgctgg atgcaaaacg 21000 aaagacgagt tctgggaagt tctcatgaat ggaaaagttg agagcaaagt gatcagcgac 21060 aaacgtcttg gttcaaatta ccgtgctgaa cactacaaag ctgaaaggag caagtatgct 21120 gacaccttct gcaatgagac atatggaact cttgatgaga acgaaataga caatgagcat 21180 gaactccttt tgaaccttgc caaacaagcc ttggctgaaa catctgtcaa agattcaaca 21240 agatgtggca tagtttctgg atgcctcagc ttcccgatgg acaacctcca aggtgaactc 21300 ttgaatgtgt accagaacca tgttgagaag aaacttggtg ccagagtttt caaagatgcc 21360 tctcactggt cagaaagaga acagtccaac aaaccagagg ctggtgacag aagaatcttc 21420 atggaccctg cttcctttgt tgctgaggaa ctcaatcttg gcgctctcca ctactctgtt 21480 gatgctgcct gtgccactgc gctctatgtg ctcagactgg cgcaagatca cttggtctct 21540 ggtgcagctg atgtgatgct ttgtggtgca acatgcctgc cggagccgtt tttcatactc 21600 tctgggttct caaccttcca agccatgcct gttggaacgg gtcagaatgt gtccatgccg 21660 ctgcacaaag attctcaagg attgacccct ggtgaaggag gttccatcat ggtcctcaaa 21720 cgtttggatg acgcaatcag agatggagat cacatctatg gaacactttt gggagccaat 21780 gtcagcaaca gtggcactgg tctgccactt aaaccccttt tgccgtcaga gaaaaagtgc 21840 ctcatggaca cctacacacg tatcaatgtg catccccaca agattcagta tgttgaatgc 21900 catgcaactg ggactccaca aggtgatcgt gtggaaattg atgctgtgaa ggcttgcttt 21960 gaaggcaaag tcccacgttt tggaaccaca aaagggaact ttggacacac attggtggca 22020 gctgggtttg ctggcatgtg caaagtgctt ctctccatga agcacgggat cattccaccc 22080 actccgggaa tagatgacga aacgaagatg gaccctctgg ttgtctctgg tgaggccatt 22140 ccctggccag aaacgaatgg agaaccgaag cgtgctggtc tctcagcgtt tggatttggt 22200 gggaccaatg ctcatgctgt ctttgaggaa catgacccaa gcaatgctgc atgcactggc 22260 catgactcca tttctgctct ctctgcacgt tgtggtggtg agtcaaacat gagaatagca 22320 atcactggga tggatgccac ttttggtgct ttgaaaggat tggatgcttt tgaaagggcc 22380 atctacactg gtgctcatgg tgctatacca ctcccagaaa agagatggag attcctgggg 22440 aaagacaagg acttccttga tctttgtgga gtcaaagcaa caccgcatgg ctgttacatt 22500 gaagatgttg aagttgattt ccagaggctg aggactccaa tgactcctga agatatgctc 22560 ttgcctcaac agcttctggc tgtcaccaca attgataggg ccatattgga ctctggcatg 22620 aagaaaggtg gaaatgtggc tgtctttgtg gggttgggga ccgatcttga actctaccgt 22680 caccgtgcaa gggttgctct caaggagaga gtgaggcctg aagccagcaa gaaactcaat 22740 gacatgatgc agtacatcaa tgattgtggg acaagcactt cttacaccag ctacataggc 22800 aacttggttg caacaagggt cagttcacaa tggggcttca cgggaccttc cttcacaatc 22860 acagaaggca acaattctgt gtacagatgt gctgaacttg gcaaatacct cttggaaacg 22920 ggagaggttg atggggttgt ggttgcgggt gttgatctct gtgggagtgc tgagaacctt 22980 tacgtcaagt caaggcgttt caaggtgtct acatctgaca ccccgagagc cagctttgat 23040 gcagctgctg atggttactt tgttggagag ggctgtggtg catttgtgct caagcgtgag 23100 acaagctgca ccaaggatga cagaatctat gcttgcatgg atgccatagt tcctggaaat 23160 gttccatctg catgtttgag agaggcattg gatcaagcca gagtcaagcc tggagacatc 23220 gagatgttgg agctttctgc tgattcagcg aggcatttga aggacccgtc tgttctccca 23280 aaggaactca ctgctgagga agagattggc ggacttcaga cgattcttcg tgacgatgac 23340 aagctcccaa ggaatgttgc aactggaagt gtcaaggcca ctgttggtga cactggttat 23400 gcttctggtg ctgccagttt gattaaggct gcgctttgca tctacaaccg ttacctgccc 23460 agcaatggag atgactggga tgaacctgca cctgaagcac cctgggacag cacactcttt 23520 gcgtgccaga cctcaagagc ttggttgaag aaccctggtg agagacgtta tgcggctgtt 23580 tccggagtca gtgaaaccag aagttgctac agtgtgctgc tttctgaagc agagggacac 23640 tacgagagag agaacagaat cagtttggat gaggaagcac ccaagctcat tgtgttgaga 23700 gcagactccc atgaagagat tcttggaaga ttggacaaga taagggaaag gttcttgcag 23760 cccacgggtg ctgcacccag agagtctgaa ttgaaggcgc aagcccgtag gatctttctt 23820 gaactccttg gtgagaccct tgcccaagat gcagcttcat ctggaagtca gaagcccctt 23880 gctctcagct tggtctccac tccatccaag ctccagagag aggtggaact tgcggcaaaa 23940 ggcattccga gatgtctcaa gatgagacgt gattggagtt cccctgctgg aagcagatat 24000 gcgcctgagc ctttggccag tgacagagtt gccttcatgt atggagaagg aaggagccct 24060 tactatggca tcacacaaga cattcatagg atttggcctg aacttcatga ggtcatcaat 24120 gagaaaacaa accgtctctg ggctgaaggt gacagatggg tcatgccaag ggccagcttc 24180 aagagtgagc ttgagtcaca acagcaagag tttgacagaa acatgattga aatgttccgt 24240 cttggaatcc tcacctcaat tgccttcaca aacctggcga gagatgttct caacatcacg 24300 cccaaagcag cctttggact cagtttggga gagatttcca tgatctttgc tttctccaag 24360 aaaaacggtt tgataagcga tcagttgaca aaggatttga gagagtcaga tgtctggaac 24420 aaagctctgg ctgttgagtt caatgcgctc agagaagcct ggggcattcc acagtctgtc 24480 ccaaaagatg agttctggca aggttacata gtgaggggaa cgaaacaaga catagaagca 24540 gccatagcac cagactcaaa gtatgtgagg ttgaccatca tcaatgatgc caacactgct 24600 ctcatcagtg gaaagcctga tgcatgcaaa gctgcgattg ctcgtcttgg tggcaacatc 24660 cctgcgcttc cagtcactca aggcatgtgt gggcattgcc cagaggtggg accttacacc 24720 aaggacatcg caaagatcca cgccaacctt gagttccctg tggttgatgg tcttgatctc 24780 tggactacca taaaccagaa gaggcttgtg ccaagagcca cgggtgccaa agatgaatgg 24840 gctccttcat cctttgggga gtatgctgga cagctctatg agaaacaagc caacttccct 24900 cagatagttg aaaccatcta caagcagaac tatgatgtgt ttgttgaagt tgggccaaac 24960 aatcacagaa gcacagcagt gagaaccacg cttggaccac agagaaacca ccttgctggt 25020 gccatagaca agcagaacga ggatgcttgg acgacaatag tcaagctcgt ggcttcattg 25080 aaggctcacc ttgttcctgg agtgaccatc agtccgctct accactccaa gctcgttgca 25140 gaggctgagg cttgttatgc tgcgctctgt aaaggtgaga aacccaagaa aaacaagttt 25200 gtgagaaaga ttcagttgaa tggcagattc aacagcaagg cagaccccat ctcctcagca 25260 gaccttgcat ccttcccacc tgcggaccct gcgattgaag ctgccatctc ttcaaggatc 25320 atgaagcctg tggctccgaa attctatgcg cgtctcaaca ttgatgagca agatgagacc 25380 agagatccga tcttgaacaa ggacaatgcg ccttccagct ccagctcttc cagcagttcc 25440 tcttccagca gttcatctcc gtctcctgct ccttcagctc cagttcagaa gaaagctgcc 25500 ccagctgcgg agacaaaagc tgttgccagt gctgatgcac ttcgtagtgc cctgcttgat 25560 cttgactcca tgcttgcgct cagctctgca agtgcttctg gcaaccttgt tgagactgcg 25620 ccatctgatg ccagtgtcat tgtccctccg tgcaacattg ctgatcttgg cagcagagct 25680 ttcatgaaaa cttatggtgt ttctgcgcct ctgtacaccg gtgcaatggc caagggaatt 25740 gcctctgcgg acttggtgat cgcagctgga aggcaaggaa tccttgcgtc atttggtgct 25800 ggaggccttc caatgcaagt tgtgcgtgag tccattgaaa agattcaagc tgccctgccc 25860 aatggaccat atgctgtcaa cctcatccat tctccttttg acagcaacct tgagaaggga 25920 aatgttgatc tcttcttgga aaagggtgtc acttttgttg aggcttcagc cttcatgacg 25980 ttgacaccgc aagttgtcag atacagagcg gctgggttga cgaggaatgc agatgggtca 26040 gtcaacataa ggaacagaat cattgggaaa gtctccagaa ctgagctggc tgaaatgttc 26100 atgcgtcctg cacctgagca tcttttgcag aagctcattg cttctgggga gatcaatcaa 26160 gaacaagctg agttggccag acgtgttcct gtggctgatg acattgcggt tgaagctgat 26220 tctggtggac acactgacaa ccgtccaatc catgtcattc tgcctctgat tatcaacctg 26280 agggataggc ttcacagaga gtgtggatac ccagcaaacc tgagggttcg tgttggtgct 26340 gggggtggca ttgggtgtcc ccaagctgcg ctggcaactt tcaacatggg tgcctctttc 26400 attgtcactg gaacagtgaa ccaagttgcc aaacaatctg ggacgtgtga caatgtgagg 26460 aaacagcttg cgaaagcaac ttactcagat gtttgcatgg caccggcagc tgacatgttt 26520 gaggaaggtg tgaaacttca agttttgaag aaaggaacaa tgttcccttc aagagccaac 26580 aaactctatg agttgttctg caagtatgat tcttttgagt ccatgcctcc agcagagctt 26640 gcgagggttg agaaaagaat cttcagtcgt gcattggaag aggtctggga tgagaccaag 26700 aacttctaca tcaaccgtct ccacaaccca gagaagatac agagggctga gagggacccc 26760 aaactgaaga tgtcactgtg tttcagatgg tatctgtccc tggcttcaag gtgggccaac 26820 actggagctt ctgacagagt catggactac caagtttggt gtggtcctgc cattggttca 26880 ttcaatgatt tcatcaaagg aacttacctt gatccggcag tggcaaatga gtacccgtgt 26940 gttgtgcaga tcaacaaaca gattcttaga ggagcgtgct tcttgaggcg tcttgaaata 27000 ctgagaaatg caaggctttc agatggtgca gctgcacttg tggcgagcat tgatgacaca 27060 tatgtccctg ctgagaagct gtgagtagtt agcttaatca cttaggtcac ctcgagtatc 27120 aaaatctatt tagaaataca caatattttg ttgcaggctt gctggagaat cgatctgcta 27180 tcataaaaat tacaaaaaaa ttttatttgc ctcaattatt ttaggattgg tattaaggac 27240 gcttaaatta tttgtcgggt cactacgcat cattgtgatt gagaagatca gcgatacgaa 27300 atattcgtag tactatcgat aatttatttg aaaattcata agaaaagcaa acgttacatg 27360 aattgatgaa acaatacaaa gacagataaa gccacgcaca tttaggatat tggccgagat 27420 tactgaatat tgagtaagat cacggaattt ctgacaggag catgtcttca attcagccca 27480 aatggcagtt gaaatactca aaccgcccca tatgcaggag cggatcattc attgtttgtt 27540 tggttgcctt tgccaacatg ggagtccaag gttggcgcgc cgaattcgcg gccgcttaat 27600 taatctgacc tgaccactag acctgagtga gtacagatgt gctactagtg ctagcctcga 27660 ggtcgaccaa cttttctata caaagttgtc taggatctgc ttttacatgc gagacacatc 27720 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 27780 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 27840 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 27900 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 27960 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 28020 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 28080 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 28140 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 28200 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 28260 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 28320 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 28380 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 28440 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 28500 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 28560 ccaaaaatgg cgcttcgtgt caagaccaac aagaagccat gttgggagat gaccaaggag 28620 gaactgacct ctggcaaaac tgaggtgttc aactatgagg aactcctgga gtttgcagag 28680 ggtgacatag ctaaggtttt tggtccggag tttgctgtca tagacaagta ccccagaagg 28740 gtgaggctgc ctgctcgtga atacctgctt gtgacaaggg tcacactcat ggatgccgag 28800 gtcaacaact acagagttgg tgccagaatg gtcactgagt atgatcttcc agtgaatggc 28860 gaattgtctg agggaggtga ttgtccctgg gcagttctgg ttgagagtgg acagtgcgat 28920 ttgatgctga tatcatacat gggaattgat ttccagaacc aaggtgacag agtttaccgt 28980 ctgctcaaca ccacgttgac attctatggg gttgcccatg agggagagac ccttgagtat 29040 gacatcagag tgaccggttt tgccaaacgt ttggatggtg gcatctccat gttcttcttt 29100 gagtatgact gctatgtcaa tgggaggctt ctcattgaga tgagagatgg ctgtgctggc 29160 ttcttcacaa atgaggagct tgatgcggga aagggtgtgg tgttcacaag aggggatttg 29220 gctgccagag ctaagattcc aaagcaagat gtttctccct atgctgtcgc tccctgcctt 29280 cacaaaacaa aactcaacga aaaggagatg cagaccttgg ttgacaaaga ttgggcttcc 29340 gtttttggct caaagaatgg catgccggag atcaactaca aactctgcgc acgtaagatg 29400 ctcatgattg atagagtcac cagcattgac cataagggag gtgtgtacgg tttgggtcag 29460 ttggttggtg agaaaatctt ggagagggac cattggtact tcccatgcca ttttgttaag 29520 gatcaagtca tggctggatc tcttgtcagc gacggttgtt cacaaatgct caagatgtac 29580 atgatctggc tcggtttgca ccttaccacc ggaccatttg acttcagacc cgtcaacgga 29640 cacccgaaca aagttaggtg taggggtcag atatctcctc ataagggaaa gttggtctac 29700 gtcatggaga tcaaagagat gggattcgac gaggataacg atccgtacgc cattgctgac 29760 gtcaacatca ttgacgttga tttcgaaaag ggtcaagatt tcagccttga tagaatcagc 29820 gactacggaa agggggatct taacaaaaag atcgtcgtgg acttcaaggg cattgctctc 29880 aagatgcaga agagatccac gaacaagaac ccctccaaag ttcagcccgt ttttgctaac 29940 ggtgcagcca ctgtgggacc ggaagcgtcc aaggccagct ctggtgccag tgctagtgct 30000 tctgctgccc ctgcgaagcc tgctttctca gcagatgttc ttgcgccaaa gccagttgca 30060 cttcctgaac atatcctcaa gggtgacgct ctcgctccga aagaaatgtc ctggcatccc 30120 atgtcaaaac ttcctggcaa cccgacgccg ttcttctccc ctagttctta ccctccgagg 30180 gcaatttgct tcatcccttt ccctggcaac ccacttgaca acaattgcaa agctggagaa 30240 atgcctctga actggtacaa catgtcagag ttcatgtgtg gaaaagttag caactgcttg 30300 ggaccagagt tcgcaagatt tgataagtca aatacgagca gaagccctgc ttttgatttg 30360 gctcttgtga ctagagttgt tgaagtcaca aacatggaac atggcaagtt cctgaatgtt 30420 gattgcaatc ccagcaaagg cacaatggtg ggggagtttg actgtcctca agatgcgtgg 30480 ttctttgatg gttcttgcaa tgatgggcac atgccttatt ccatcatcat ggaaatcgga 30540 cttcagacat ccggtgttct caccagtgtg ttgaaagcac cactgacgat ggacaaagat 30600 gacattctct ttaggaacct tgatgcaagt gctgaaatgg tgaggccaga tgtggatgtg 30660 agggggaaaa ccataaggaa tgtgaccaag tgcactggat atgcaatgtt gggaaagatg 30720 gggattcaca gattcacgtt tgagttgtct gttgatggcg tggttttcta caaaggaagc 30780 acttcctttg gatggttcac tccagaggtg tttgctcagc aagctggact tgacaatggg 30840 aaaaagacgg agccttggtg caaaactaac aacacgagtg tgagaagagt tgaaatcgca 30900 tcagccaaag gcaaagaaca gctgactgag aaacttccag atgcaactaa tgctcaagtt 30960 ctcagaaggt cagaacagtg tgagtatttg gattacctca acattgctcc tgattctggg 31020 ctgcatggga aaggatatgc gcatggacac aaagatgtta acccacaaga ctggttcttc 31080 tcttgtcact tttggtttga tcctgtgatg cctggatctt tgggaattga gtcaatgttc 31140 cagctcattg aggcatttgc ggtggaccag aacataccgg gagaatacaa tgtgagcaat 31200 ccgacctttg cccatgcccc tggaaaaacg gcgtggaagt accgtggtca attgacacca 31260 aagaaccgtg cgatggactg tgaggtgcac atagtttcaa tcacagcctc tcctgaaaat 31320 ggaggctacg ttgacatagt ggcagatgga gcactttggg ttgatggtct ccgtgtgtat 31380 gaagccaaag aactcagagt tcgtgttgtc agtgcaaaac ctcaagccat tccggatgtt 31440 caacaacagc cacccagcgc aaaagcggac cctgggaaaa ctggagttgc actcagtccg 31500 actcagttga gagatgtcct gcttgaagtg gacaatccat tgtatcttgg tgttgaaaac 31560 tccaatttgg tgcagtttga atccaaacct gcaacttctt caaggattgt cagtatcaaa 31620 ccatgctcca taagtgatct tggtgacaag tccttcatgg aaacgtacaa tgtgtcagca 31680 cctctgtaca ctggagcaat ggccaaggga attgcgtctg cggaccttgt cattgctgct 31740 gggaagagga agatattggg ttcttttgga gcgggtgggc ttccaatgca ccatgtgagg 31800 gcagctttgg agaagataca agcggctctg cctcaaggac catatgctgt gaacctcatt 31860 cattcacctt ttgacagcaa cttggaaaag gggaatgttg atctcttcct cgaaaagggc 31920 gttactgtgg tggaggcttc tgcattcatg accctcactc cccaagttgt tcgttaccgt 31980 gcagccggac tctcaaggaa cgctgatggg agtgtcaaca tcagaaaccg tatcattgga 32040 aaagtgtcca gaacagaact cgccgaaatg ttcatcagac cggctccgga acacctcctt 32100 gagaaactca tagcttctgg tgagataaca caagaacaag ccgaacttgc ccgtcgtgtt 32160 cctgtcgcag acgacatcgc tgttgaagct gattctggtg gtcacactga caaccgtccc 32220 atccacgtca tcttgccttt gatcatcaac cttaggaaca gactgcacag agagtgtggt 32280 tatcctgcgc accttagagt cagagttgga gctggaggcg gtgttggctg cccacaagct 32340 gctgcggctg ccctcacaat gggagctgcc ttcatagtga ctgggactgt caatcaagtt 32400 gctaaacagt ccgggacatg tgacaacgtg agaaaacaac tcagtcaagc cacctacagt 32460 gacatttgca tggctccagc agctgatatg tttgaggaag gtgtgaaact ccaagttctc 32520 aagaagggaa caatgttccc atcaagagct aacaaattgt acgaactctt ttgcaagtac 32580 gactctttcg attcaatgcc tcctgctgaa cttgaaagga tcgagaagag gatcttcaag 32640 agggcacttc aagaggtctg ggaagagacc aaggactttt acatcaatgg tttgaagaat 32700 ccagagaaga tacagagagc ggaacatgac cccaaactca agatgtcttt gtgtttcaga 32760 tggtatcttg gtcttgcatc acgttgggcc aacatgggtg ctcctgatcg tgtcatggac 32820 tatcaagtct ggtgtggtcc agccattgga gccttcaatg acttcatcaa aggaacatat 32880 cttgatcctg ctgtctctaa tgagtaccca tgtgttgtcc agatcaacct tcagattttg 32940 cgtggtgcat gttacctgag gcgtcttaat gctctgagaa atgatccgag gattgatctt 33000 gagactgaag atgctgcctt tgtctatgag ccaacgaatg cgctttgagt agttagctta 33060 atcacttagg tcacctcgag tatcaaaatc tatttagaaa tacacaatat tttgttgcag 33120 gcttgctgga gaatcgatct gctatcataa aaattacaaa aaaattttat ttgcctcaat 33180 tattttagga ttggtattaa ggacgcttaa attatttgtc gggtcactac gcatcattgt 33240 gattgagaag atcagcgata cgaaatattc gtagtactat cgataattta tttgaaaatt 33300 cataagaaaa gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg 33360 cacatttagg atattggccg agattactga atattgagta agatcacgga atttctgaca 33420 ggagcatgtc ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca 33480 ggagcggatc attcattgtt tgtttggttg cctttgccaa catgggagtc caaggttggc 33540 gcgccgaatt cgcggccgct taattaatca cgagtggatg actgtcacga ctgactcatg 33600 actgactact agtgctagcc tcgaggtcga cacaactttg tataataaag ttgtctagac 33660 ctaggatctg cttttacatg cgagacacat cttctaaagt aattttaata atagttacta 33720 tattcaagat ttcatatatc aaatactcaa tattacttct aaaaaattaa ttagatataa 33780 ttaaaatatt acttttttaa ttttaagttt aattgttgaa tttgtgacta ttgatttatt 33840 attctactat gtttaaattg ttttatagat agtttaaagt aaatataagt aatgtagtag 33900 agtgttagag tgttacccta aaccataaac tataagattt atggtggact aattttcata 33960 tatttcttat tgcttttacc ttttcttggt atgtaagtcc gtaactggaa ttactgtggg 34020 ttgccatgac actctgtggt cttttggttc atgcatggat cttgcgcaag aaaaagacaa 34080 agaacaaaga aaaaagacaa aacagagaga caaaacgcaa tcacacaacc aactcaaatt 34140 agtcactggc tgatcaagat cgccgcgtcc atgtatgtct aaatgccatg caaagcaaca 34200 cgtgcttaac atgcacttta aatggctcac ccatctcaac ccacacacaa acacattgcc 34260 tttttcttca tcatcaccac aaccacctgt atatattcat tctcttccgc cacctcaatt 34320 tcttcacttc aacacacgtc aacctgcata tgcgtgtcat cccatgccca aatctccatg 34380 catgttccaa ccaccttctc tcttatataa tacctataaa tacctctaat atcactcact 34440 tctttcatca tccatccatc cagagtacta ctactctact actataatac cccaacccaa 34500 ctcatattca atactactct aggatccaca accaaaaatg cccactccag atgccactgc 34560 gcctctcaac aagccgtcag actatgctgt gtaccacgaa gaggatggtc ctttctggac 34620 tgctgattcc tctggtgtca gcagagtgaa cttctctgaa actggtgtgg gatctgaagg 34680 tgtcattcct gcgctcacac tgattgatgt gtttgagcgt gcggtcaaac gtggtggcaa 34740 taggattgca ttcagaacag agaacatgcc aaccttgagg cgtggagaag aggctcctga 34800 tgctttgccg ctcaaagatt ggaagtcctg gtcttggaaa cagtacaaag ctgatgttca 34860 cagaattgcg agggctctga tggatttggg agttgagcag cacgatgcgg tctccatctt 34920 tggcttcaac tcaccagaat ggttcctcag tgctgtcggt gcagttcatg ctggtgccaa 34980 gatcgctgga atctaccctt cagacactcc tgctcaagtg cagtacaaag ccttccactc 35040 tgacactgct gttgcagttg tggagaacga acagtgcttc aagaaattcg cagaggtggt 35100 tgaggatctc ccttacctca aagccattgt ttgttgggat tatgaagcca cagacatcac 35160 aagggaagat ggttcagtgg ttgaagtcct gacctttgct gagttcctga agagaggtga 35220 cactgtggaa gcagctgcac ttgatgagag aatcagcaag attgagccca ccatgtgtgc 35280 tgccctgatc tacaccagtg gcacaactgg gagacccaaa gctgtcatga tctcccatga 35340 caaccttgtc tttgaagcat ctgctgtggt cccgaacttg ggaggggcat gcacgaccac 35400 tgctgaggaa aggattcttt cctaccttcc tttgtctcat gttgctggaa tgatggttga 35460 catcattgct ccaatcatag cgacagcgtt ccacaaagga aggatctgtg tttgttttgc 35520 cagaccgtat gatttgagaa ctggaaccct tgggcaaaga ctcaatgcag tggagccaac 35580 catattcctt ggtgtgccac gtgtgtggga aaagatccaa gagaaattga tggctgttgg 35640 tgccaaaacc actggtctca agaagaaact cagcacggca gccaagaaac gtgggcttga 35700 atttcaagag gaacaacaga tagggagaag tggtgccaac cctggctttg gaccgcttgg 35760 aatctacaag aaacttttgg gcctcatcaa aggaaagttg gggctgacca aatgcaagtt 35820 tgcctttgct ggcgcagctc caatgacccg tgaaaccctt cagtactttg gagcgctgaa 35880 catcaacatc aatgaggtct atggaatgtc tgaatgcagt ggggctgcca cctggtcaac 35940 tgacaaagct catgagtggg gcactgttgg ctatgagatg ccctcctgtg aagtcagagt 36000 gttcaagatt gcggaggatg gcaccaagac agagtgccca agagctgctg acatcatgca 36060 tgccacagaa gaggaacaag gtgaagtttg cttccgtgga aggaacatca tgatgggata 36120 cttggccaac ccgaaacttg gtgatgacca tgttgctgag attgaagaga agaatgcagc 36180 tgccatagat agcgaagggt ggcttcactc tggtgacaag ggagcaattt ccacaagggg 36240 aatgctcaag ataacgggca gatacaagga gcttatcata ggtgctggtg gggagaatgt 36300 ggctcctgtt cccattgaag atgccatcaa ggcgaggatg ccttttgttt caaatgcaat 36360 gatggttgga gacaagagga agttcatggc tgtccttctg actttgaaaa cggttggagc 36420 aactggagag cttcctggca caaacaagct catgggagca gctgcggact atggtgagac 36480 cattgaggat gcctgtgaca atgaggcgct cattgaagag ataacgcagc aactcaaaga 36540 gataggtgac gatggagatg tcacgccaag caatgcagcc cgtatacaaa agttcacgat 36600 tttgccactc gacttcagtg tctccacaga tgaactgaca gcaacgttga aattgaagag 36660 aagtgtggtt gcagacaagt atgaggacat catagaggct ttctatgaga gcaagtctgt 36720 ctttgtgcct tactcaacag ttggagctta tgcaacgggt ggaccagttg atgactctgt 36780 tgtggatgga tcattcaaag gtgactttag catgattggg gatgacgatc cggatcttca 36840 gaatgtggat gtccttgaag caattgatga ggacaactga gtagttagct taatcactta 36900 ggtcacctcg agtatcaaaa tctatttaga aatacacaat attttgttgc aggcttgctg 36960 gagaatcgat ctgctatcat aaaaattaca aaaaaatttt atttgcctca attattttag 37020 gattggtatt aaggacgctt aaattatttg tcgggtcact acgcatcatt gtgattgaga 37080 agatcagcga tacgaaatat tcgtagtact atcgataatt tatttgaaaa ttcataagaa 37140 aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca cgcacattta 37200 ggatattggc cgagattact gaatattgag taagatcacg gaatttctga caggagcatg 37260 tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg caggagcgga 37320 tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaaggttg gcgcgccgaa 37380 ttcgcggccg cttaattaat ctgatcatgg atgctacgtc acggcagtac aggactatca 37440 ctagacctag gatctgcttt tacatgcgag acacatcttc taaagtaatt ttaataatag 37500 ttactatatt caagatttca tatatcaaat actcaatatt acttctaaaa aattaattag 37560 atataattaa aatattactt ttttaatttt aagtttaatt gttgaatttg tgactattga 37620 tttattattc tactatgttt aaattgtttt atagatagtt taaagtaaat ataagtaatg 37680 tagtagagtg ttagagtgtt accctaaacc ataaactata agatttatgg tggactaatt 37740 ttcatatatt tcttattgct tttacctttt cttggtatgt aagtccgtaa ctggaattac 37800 tgtgggttgc catgacactc tgtggtcttt tggttcatgc atggatcttg cgcaagaaaa 37860 agacaaagaa caaagaaaaa agacaaaaca gagagacaaa acgcaatcac acaaccaact 37920 caaattagtc actggctgat caagatcgcc gcgtccatgt atgtctaaat gccatgcaaa 37980 gcaacacgtg cttaacatgc actttaaatg gctcacccat ctcaacccac acacaaacac 38040 attgcctttt tcttcatcat caccacaacc acctgtatat attcattctc ttccgccacc 38100 tcaatttctt cacttcaaca cacgtcaacc tgcatatgcg tgtcatccca tgcccaaatc 38160 tccatgcatg ttccaaccac cttctctctt atataatacc tataaatacc tctaatatca 38220 ctcacttctt tcatcatcca tccatccaga gtactactac tctactacta taatacccca 38280 acccaactca tattcaatac tactctagga tccacaacca aaaatgcttc agcacacttg 38340 gcttccgaag cctcccaatc tgaccctctt gtcagatgag gttcatctct ggaggattcc 38400 tcttgaccag cctgagtcac aacttcaaga ccttgctgcc accctgagca gtgatgaatt 38460 ggcgagggca aacagattct actttccaga acacagaagg cgtttcactg ctgggagagg 38520 catcctcaga tccatcttgg gtggatactt gggagtggaa ccgggtcaag tcaagtttga 38580 ttatgagtcc cgtgggaaac cgatccttgg tgacagattt gctgagagtg gactcctgtt 38640 caacttgtct cacagccaga accttgcctt gtgtgctgtc aactacacgc gtcaaatagg 38700 cattgatctt gaatatctgc gtccaacatc tgacttggag tctcttgcaa agaggttctt 38760 tctcccaaga gaatatgaac tcttgaggtc actccctgat gagcagaaac agaagatttt 38820 ctttcgttac tggacttgca aagaggctta tctcaaagca acgggagatg gaatagccaa 38880 acttgaagag atcgagatag cactcacccc aacagaacct gccaagctcc aaacagctcc 38940 tgcgtggtct ctgttggagc ttgtgccaga tgacaattgt gttgcagctg tggctgttgc 39000 gggttttggt tggcagccca agttctggca ttactgagta gttagcttaa tcacttaggt 39060 cacctcgagt atcaaaatct atttagaaat acacaatatt ttgttgcagg cttgctggag 39120 aatcgatctg ctatcataaa aattacaaaa aaattttatt tgcctcaatt attttaggat 39180 tggtattaag gacgcttaaa ttatttgtcg ggtcactacg catcattgtg attgagaaga 39240 tcagcgatac gaaatattcg tagtactatc gataatttat ttgaaaattc ataagaaaag 39300 caaacgttac atgaattgat gaaacaatac aaagacagat aaagccacgc acatttagga 39360 tattggccga gattactgaa tattgagtaa gatcacggaa tttctgacag gagcatgtct 39420 tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc ccatatgcag gagcggatca 39480 ttcattgttt gtttggttgc ctttgccaac atgggagtcc aaggttggcg cgccgaattc 39540 gcggccgctt aattaactac tgtcactgag gccgtagacg agtacggact gatctaacta 39600 gtgctagcct cgaggtcgac acccagcttt 39630 <210> 57 <211> 36936 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7733 <400> 57 gatctgcttt tacatgcgag acacatcttc taaagtaatt ttaataatag ttactatatt 60 caagatttca tatatcaaat actcaatatt acttctaaaa aattaattag atataattaa 120 aatattactt ttttaatttt aagtttaatt gttgaatttg tgactattga tttattattc 180 tactatgttt aaattgtttt atagatagtt taaagtaaat ataagtaatg tagtagagtg 240 ttagagtgtt accctaaacc ataaactata agatttatgg tggactaatt ttcatatatt 300 tcttattgct tttacctttt cttggtatgt aagtccgtaa ctggaattac tgtgggttgc 360 catgacactc tgtggtcttt tggttcatgc atggatcttg cgcaagaaaa agacaaagaa 420 caaagaaaaa agacaaaaca gagagacaaa acgcaatcac acaaccaact caaattagtc 480 actggctgat caagatcgcc gcgtccatgt atgtctaaat gccatgcaaa gcaacacgtg 540 cttaacatgc actttaaatg gctcacccat ctcaacccac acacaaacac attgcctttt 600 tcttcatcat caccacaacc acctgtatat attcattctc ttccgccacc tcaatttctt 660 cacttcaaca cacgtcaacc tgcatatgcg tgtcatccca tgcccaaatc tccatgcatg 720 ttccaaccac cttctctctt atataatacc tataaatacc tctaatatca ctcacttctt 780 tcatcatcca tccatccaga gtactactac tctactacta taatacccca acccaactca 840 tattcaatac tactctagga tccacaacca aaaatggcag cgaggctgca agagcagaaa 900 ggaggtgaga tggacacgag gattgcgatc ataggcatga gcgccatact tccttgtgga 960 accacggtga gagagtcatg ggagaccata agggctggca ttgattgcct gtctgatctt 1020 cctgaggatc gtgttgatgt gactgcgtac tttgacccag tcaagaccac aaaggacaaa 1080 atctactgca agagaggagg tttcattcct gagtatgact ttgatgcgcg tgagtttggt 1140 ctcaacatgt tccagatgga agattctgat gcgaatcaga ccatctcact tctcaaggtc 1200 aaagaagcac ttcaagatgc tgggattgat gcacttggaa aggagaagaa aaacatagga 1260 tgcgtgcttg gcattggagg tggacagaag agttcacatg agttctacag cagactcaac 1320 tatgtggttg tggagaaagt cttgagaaag atggggatgc cagaggaaga tgtcaaagtg 1380 gctgtggaga agtacaaagc gaacttccca gaatggagac ttgactcttt ccctggattc 1440 cttgggaatg tcactgctgg cagatgcacc aacacattca acttggatgg catgaactgc 1500 gttgtggatg ctgcatgtgc aagctccttg atagcagtca aggttgcgat tgatgaactg 1560 ctctatggtg actgcgacat gatggtcact ggtgcaacct gcacagacaa ctccattggg 1620 atgtacatgg ctttctcaaa gacacctgtg ttcagcacgg acccaagtgt gagagcgtat 1680 gatgagaaaa cgaaagggat gctcattggt gagggatcag ccatgttggt gctcaaacgt 1740 tatgcagatg cagtgaggga tggtgatgag attcatgctg tgattcgtgg ttgtgcaagc 1800 tcaagtgatg gcaaagcagc tggcatctac actcccacaa tcagtggtca agaggaagcc 1860 ttgagacgtg catacaatcg tgcctgcgtt gaccctgcca ctgtcacttt ggttgagggt 1920 catgggactg gcactccagt tggagatagg attgagttga ctgcgttgag gaacttgttt 1980 gacaaagcgt atggtgaagg gaacacagag aaagttgcag ttggaagtat caagtccagt 2040 attggtcatt tgaaggctgt tgctggtctt gctggaatga tcaaggtcat catggctctc 2100 aaacacaaga ctttgcctgg aacgatcaat gtggacaatc cacccaatct ctatgacaac 2160 actcccatca atgagtcatc cctctacatc aacacgatga acagaccctg gttccctcca 2220 cctggtgttc cgagacgtgc tggaatatcc agctttggct ttggaggtgc caactaccat 2280 gcagtgttgg aagaggctga accagagcac acgacagcgt acagactcaa caaacgtcca 2340 caaccggtgt tgatgatggc tgcaactcct gctgcgttgc agagcctttg tgaagctcag 2400 ctgaaagagt ttgaagctgc gatcaaggag aatgagacag tcaagaatac tgcctacatc 2460 aagtgtgtca agtttggaga acagttcaag ttccctggga gcatacctgc gaccaatgcg 2520 agactgggat tcttggtcaa agatgcggag gatgcgtgct ccacgcttcg tgctatatgt 2580 gctcagtttg ccaaagatgt gaccaaagaa gcgtggaggc ttccgagaga aggagtttct 2640 ttccgtgcca aaggcattgc caccaatggt gctgttgctg cactcttctc tggacaaggt 2700 gctcagtaca cacacatgtt cagtgaagtt gcgatgaact ggcctcagtt cagacagtcc 2760 attgctgcaa tggacgctgc acagtccaag gttgctggaa gtgacaaaga ctttgaaaga 2820 gtctctcaag tcctgtaccc aaggaaaccg tacgaacgtg agccagaaca agatcacaag 2880 aaaatctcac tcacagcata cagccagccg tcaaccctgg catgtgctct tggtgccttt 2940 gagattttca aggaagctgg attcactcca gactttgcag ctggacacag cttgggtgag 3000 tttgctgcgc tctatgcagc tggttgtgtt gacagagatg agttgtttga acttgtttgc 3060 agaagggcaa ggatcatggg aggcaaagat gcacctgcca cacccaaagg ttgcatggca 3120 gctgtcatag gacccaacgc agagaacatc aaggttcaag ctgccaacgt ctggcttgga 3180 aactcaaact ctccgtctca gactgtcatt actgggtctg ttgaagggat tcaagctgaa 3240 agtgccagat tgcagaagga agggttcaga gttgtgcctc ttgcttgtga gtctgccttc 3300 cattctccac agatggaaaa tgccagctca gctttcaaag atgtcatatc caaggtttcc 3360 ttccgtacac ccaaagcaga aacaaagctc ttcagcaatg tttctggaga gacctatccg 3420 acggatgcca gagagatgct cactcaacac atgacctcat ctgtgaagtt cctgacccaa 3480 gtgaggaaca tgcaccaagc tggtgcgaga atctttgttg agtttggacc gaaacaagtt 3540 ctcagcaaac ttgtttctga aaccctcaaa gatgacccgt ctgttgtcac agtgtctgtg 3600 aacccagctt ctggcacgga ctctgacata cagttgaggg atgctgcagt tcagctggtt 3660 gtggctggtg tcaatcttca agggtttgac aagtgggatg ctcctgatgc cacgaggatg 3720 caagccatca agaaaaagag aaccacactc agactttcag ctgccaccta tgtcagtgac 3780 aagaccaaga aagtgaggga tgctgccatg aacgacggaa gatgtgtcac atacctcaag 3840 ggtgctgcac cactcatcaa ggctcctgaa cctgtggttg acgaagctgc caagagggag 3900 gctgaaaggc ttcagaagga gcttcaagac gctcagaggc agcttgacga cgccaaacgt 3960 gcagctgcag aagccaactc caaacttgct gctgccaagg aggaagccaa gactgctgca 4020 gcttcagcca agcctgcagt tgacactgct gttgtggaga aacatcgtgc catcctcaag 4080 tccatgctgg ctgagctgga tggttatgga agtgttgatg cttcttccct tcagcaacag 4140 caacagcaac agactgctcc ggcgcctgtt aaagccgctg cccctgccgc tcccgttgca 4200 agtgctcctg ctcccgccgt gtccaacgag ttgcttgaga aagctgagac agttgtgatg 4260 gaggttctcg ctgccaagac tggatacgaa acagacatga ttgaggcgga catggagctt 4320 gagactgagt tgggaattga cagcatcaag agggttgaaa ttctttctga agtccaagct 4380 atgcttaacg ttgaggcgaa agatgttgat gctctctcta ggacgagaac agtgggtgaa 4440 gttgtcaacg ctatgaaggc agaaatcgct ggttcttccg ctccggcgcc ggcagctgcc 4500 gcacctgcac cggctaaagc ggctccagct gcagctgcac cagctgtttc taatgagctg 4560 ctcgaaaagg cagaaactgt tgtgatggaa gtccttgctg ccaaaactgg ctacgaaact 4620 gacatgatag aatcagacat ggagcttgag acggagcttg gaattgattc catcaagcgt 4680 gtggaaatcc tcagtgaagt ccaagccatg ctcaatgtgg aagctaagga tgtggatgct 4740 ctctcaagga ccagaactgt cggtgaggtt gtcaatgcca tgaaggctga gatagctgga 4800 ggctccgctc ctgcgccggc cgctgccgct cctggtcccg ctgccgcggc tccagctcca 4860 gctgcagccg ctccggccgt ctcaaacgaa ctccttgaaa aagctgagac cgtcgtgatg 4920 gaggtcttgg cagctaaaac cggctatgag actgacatga ttgagtctga catggaattg 4980 gagacagaac ttgggataga cagcatcaaa cgtgttgaaa tcctcagtga ggtccaagct 5040 atgctgaacg tggaagcgaa ggatgttgat gctctgtcca gaacgaggac cgttggtgag 5100 gttgtggatg ccatgaaggc tgaaattgct ggagggtctg cgcccgcgcc agctgcagcc 5160 gctcctgcac ccgcagccgc ggcacctgct ccagctgcac ctgcacctgc cgtttcttca 5220 gaactcttgg agaaggctga gacggttgtg atggaggttt tggctgcaaa gactggctat 5280 gagaccgaca tgattgaatc tgatatggaa ttggagacag agcttgggat cgattccatc 5340 aaaagagtgg agatcctgag tgaagtccaa gccatgctca atgttgaagc taaggatgtt 5400 gatgcacttt caagaacaag gacagtgggt gaggttgtgg atgccatgaa ggcggagatt 5460 gctggaggga gcgcgcctgc gccagctgcc gcggcacctg caccggcagc cgcagctcca 5520 gctccagctg caccagcacc ggccgcgccc gcgccagctg tttcatccga actcctggaa 5580 aaggccgaga cggttgtgat ggaggtgctc gcagctaaga ctggttacga gaccgacatg 5640 attgaaagtg acatggagct tgaaaccgag ttgggaatag atagcatcaa acgtgttgaa 5700 atcttgtctg aggtccaagc tatgttgaac gtggaggcaa aagatgtcga tgcgctttca 5760 agaaccagaa cagtcggtga ggtcgtggac gccatgaagg ctgaaattgc cggaagctct 5820 gctagtgcgc cagctgcagc cgcacctgca cctgctgccg cggcacctgc tccagctgca 5880 gccgctcctg ccgtcagcaa tgagttgctg gagaaagccg aaacagttgt gatggaagtc 5940 cttgctgcaa aaactggtta cgaaacggac atgattgagt cagacatgga attggagacc 6000 gaacttggaa tagattccat caagagagtg gagatcctct ctgaggtgca agccatgctc 6060 aacgttgagg ctaaagatgt tgatgcactc agtaggacaa gaactgtggg ggaagttgtc 6120 gatgcgatga aggctgagat tgctggaggc tctgctcctg cgccggcagc tgccgcacct 6180 gcacccgcag ccgcggcacc agctgtcagc aacgaattgc ttgagaaggc tgaaacagtt 6240 gtcatggaag tgctggctgc aaaaaccggc tatgagactg acatgattga gtctgacatg 6300 gaacttgaga cggaattggg cattgacagc atcaagcgtg ttgagatcct cagtgaagtc 6360 caagcaatgc tcaatgtgga ggctaaggat gtggatgccc tctcaaggac cagaacagtt 6420 ggtgaggtcg ttgatgcgat gaaggcggag atcgctggaa gttccgcgcc tgcgccagct 6480 gcagccgctc ctgcacccgc agccgcggca ccggctccag ctgcagccgc accggccgtc 6540 tcctcagaac tcctggaaaa ggctgagaca gttgtgatgg aagtccttgc tgcgaaaact 6600 ggctatgaga ccgacatgat tgaatctgac atggaactgg agaccgagct tggaattgac 6660 tccatcaaac gtgttgaaat cctctctgag gttcaagcga tgttgaatgt ggaggccaaa 6720 gatgttgatg ctctttccag aacaaggacg gtgggagagg tggttgatgc catgaaggct 6780 gagatagctg ggggttctgc tcctgcgcca gctgcagccg ctcctgctcc cgctgccgcg 6840 gcaccagctg tcagcaacga actccttgaa aaagctgaaa ctgttgtgat ggaggttttg 6900 gctgcaaaga ctggatacga gactgacatg atagagtcag acatggagtt ggaaacagag 6960 cttgggattg atagcatcaa gcgtgtggaa atcctttctg aggttcaagc catgctgaac 7020 gttgaggcca aagatgtcga tgctttgtca aggaccagaa cggttggaga agtggtcgat 7080 gccatgaagg ctgagatagc tggtggatca gcccctgctc cggctgcagc cgctcctgca 7140 agtgccggtg ctgcaccagc tgtgaagatt gattcagttc atggtgctga ctgtgacgat 7200 ttgtcactga tgcacgcaaa ggtggttgac atcagaaggc cagatgaact gattctggaa 7260 agaccggaga accgtccagt tcttgtcgtt gacgatggct cagagttgac acttgctctt 7320 gtgagggtgt tgggagcctg tgcagttgtc ttgacctttg agggattgca gttggctcag 7380 agagctggtg cagctgccat tcgtcatgtt ctggctaagg acctttcagc agaatcagct 7440 gagaaggcca tcaaagaggc tgagcaaaga tttggagcgt tgggaggctt cataagccag 7500 caagcagaaa ggtttgaacc agctgagata ctgggtttca cactcatgtg tgcgaagttt 7560 gccaaagcct ctctctgcac tgctgtggct ggaggtcgtc ctgccttcat cggtgtggct 7620 aggcttgacg gcagacttgg attcacctct caaggcactt ctgacgcact caagagagca 7680 cagcgtggag cgatctttgg actctgcaag acaatagggt tggagtggtc agaatctgat 7740 gtgttcagcc gtggtgtgga cattgctcaa gggatgcacc cagaagatgc tgcagttgcc 7800 atagttaggg agatggcgtg tgctgacatc agaatcagag aggttgggat tggagcaaac 7860 cagcaaagat gcacaatcag agctgccaag ttggagactg ggaaccctca gagacagata 7920 gcgaaagacg atgtgcttct ggtttctgga ggtgctcgtg ggatcacacc tctttgcatc 7980 cgtgagatca cgcgtcagat tgctggaggc aagtacattc tgttgggtcg tagcaaagtt 8040 tctgcgtctg aaccagcatg gtgtgctggc atcactgatg agaaggctgt gcagaaagct 8100 gccacccaag agttgaagag ggccttcagt gctggtgaag gacccaaacc cactccaagg 8160 gctgtcacga aacttgtggg atctgttctt ggtgctcgtg aagtgagaag ctccattgct 8220 gccattgaag ctctgggtgg caaagcgatc tacagctctt gtgatgtgaa ctctgctgca 8280 gatgttgcca aagcagttag ggatgcagag tcccagcttg gtgccagagt tagtggaatc 8340 gttcacgctt ctggtgtgct ccgtgatcgt ctcatagaga agaaactccc tgacgagttt 8400 gatgctgtct ttgggaccaa agtcactggg ttggagaatc ttctggctgc agttgatcgt 8460 gccaacctca aacacatggt tctcttcagc tcccttgctg gtttccacgg caatgttggt 8520 cagtctgact atgcgatggc aaacgaagct ctcaacaaga tgggtttgga acttgcgaag 8580 gatgtcagtg tcaagtccat ctgctttggt ccctgggatg gtgggatggt gacacctcaa 8640 ctcaagaaac agtttcaaga aatgggagtt cagatcattc ctcgtgaagg aggtgctgat 8700 acagttgcga ggattgtgtt gggatcaagt cctgctgaga tccttgttgg aaactggagg 8760 acaccatcca agaaagttgg aagtgacacg atcaccctgc atagaaagat cagtgccaag 8820 tccaatccct tcttggagga tcatgtcata caaggcagaa gggtgttgcc aatgaccctt 8880 gctattggga gccttgctga gacctgcctt ggactcttcc ctggctactc tctctgggcc 8940 atagatgacg cacagctctt caaaggtgtc actgttgatg gagacgtcaa ctgtgaagtg 9000 accctcacac cgtccactgc tccgagtggg agggtgaatg tccaagcgac gctcaagacg 9060 ttctcaagtg gaaaacttgt tccagcctac agagctgtca tagtgttgtc caaccaaggt 9120 gctccacctg ccaatgcaac catgcagcct ccgagccttg atgcagatcc tgctttgcaa 9180 gggtcagtct atgatggcaa gactctcttt catggtcctg cgttcagagg cattgatgac 9240 gttctcagct gcaccaagag ccagcttgtt gccaagtgtt ctgctgttcc tggatcagat 9300 gcagccagag gagagtttgc aactgacact gatgcacatg atccctttgt caatgatctt 9360 gcctttcaag caatgttggt ctgggtgagg agaacgctgg gacaagctgc actcccgaac 9420 tccattcaga ggatagtcca acacagaccg gtcccacaag acaaaccctt ctacatcacc 9480 ctcagatcca atcagagtgg tggacatagc cagcacaaac atgcacttca gttccacaac 9540 gaacaaggtg atctcttcat tgatgttcaa gcctctgtca ttgcgacaga ctcacttgcg 9600 ttctgagtag ttagcttaat cacttaggtc acctcgagta tcaaaatcta tttagaaata 9660 cacaatattt tgttgcaggc ttgctggaga atcgatctgc tatcataaaa attacaaaaa 9720 aattttattt gcctcaatta ttttaggatt ggtattaagg acgcttaaat tatttgtcgg 9780 gtcactacgc atcattgtga ttgagaagat cagcgatacg aaatattcgt agtactatcg 9840 ataatttatt tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca 9900 aagacagata aagccacgca catttaggat attggccgag attactgaat attgagtaag 9960 atcacggaat ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact 10020 caaaccgccc catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca 10080 tgggagtcca aggttggcgc gccgaattcg cggccgctta attaagatga gtgatactca 10140 ggactcagga ctcactctgc tgatcactag tgctagcctc gaggtcgaca caactttgta 10200 tacaaaagtt gtctagacct aggttaatta agcggccgca gcttatttct cttttcgata 10260 ctctaattga gccgtgcgct ctatctagac caattagaat tgatggagct ctaaaggttg 10320 ctggctgttt tcttgttcat atgattaact tctaaacttg tgtataaata ttctctgaaa 10380 gtgcttcttt tggcatatgt aggttgggca aaaacgagga agattgcttc tcaatttgga 10440 agatgatgaa cagccgaaga agaaaataag aataggcagt cctgctactc aatggatctc 10500 agtctataac ggtcgtcgtc ccatgaaaca gaggtaacac attttttgca tatacacttt 10560 gatagttcct cactaactgt gtaatctttt ggtagatatc actacaatgt tggagagaca 10620 aggctgcgcc agcatataca gaagggaaat gaagatggcc ttttgattag ctgtgtagca 10680 tcagcagcta atctctgggc tctcatcatg gatgctggaa ctggattcac ttctcaagtt 10740 tatgagttgt caccggtctt cctacacaag gtaataatca gttgaagcaa ttaagaatca 10800 atctgatttg tagtaaacta agaagaactt accttatgat ttccccgcag gactggatta 10860 tggaacaatg ggaaaagaac tactatataa gctccatagc gggttcagat aacgggagct 10920 ctttagttgt tatgtcaaaa ggttagtgtt tagtgaataa taaacttatt atcaaaaagt 10980 cttcattgac ttatttatat acttgttgtg aattggtagg aactacttat tctcagcagt 11040 catacaaagt gagtgactca tttccattca agtggataaa taagaaatgg aaagaagatt 11100 ttcatgtaac ctccatgaca actgctggta atcgttgggg tgtggtaatg tcgaggaact 11160 ctggcttctc tgatcaggta ggtttttgtc tcttattgtc tggtgttttt attttcccct 11220 gatagtctaa tatgataaac tctgcgttgt gaaaggtggt ggagcttgac tttttgtacc 11280 caagcgatgg gatacatagg aggtgggaga atgggtatag aataacatca atggcagcaa 11340 ctgcggatca agcagctttc atattaagca taccaaagcg taagatggtg gatgaaactc 11400 aagagactct ccgcaccacc gcctttccaa gtactcatgt caaggttggt ttctttagct 11460 ttgaacacag atttggatct ttttgttttg tttccatata cataggacct gagagctttt 11520 ggttgaattt tttttttttc aggacaaatg ggcgaagaat ctgtacattg catcaatatg 11580 ctatggcagg acagtgtgct gatgatacac acttaagcat catgtgttgt gttagaaagc 11640 cgaagacaat tggagcgagc ctcagggtcg tcataatacc aatcaaagac gtaaaaccag 11700 acgcagtctc tttggttgaa tgtgatgaaa gggatgtgtc ttggtatgta tgtacgagta 11760 acaaaagaga agatgcaatt gagtagtaga aagatttgag agctttttaa agcccttcaa 11820 gtgtgtgctt ttatcttatt gatatcatcc atttgcgttg tttaatgcgt ctttagatat 11880 gtttctgttt ctttctcagt gtctgaatat ctgataagtg caatgtgaga aagccacacc 11940 aaaccaaaat attcaaatct tatattttta ataatgtcga atcactcgga gttgccacct 12000 tctgtgccaa ttgtgctgaa tctatcacac taaaaaaaac atttcttcaa ggtaatgact 12060 tgtggactat gttctgaatt ctcattaagt ttttattttt tgaagtttaa gtttttacct 12120 tcttttttga aaaatatcgt tcataagatg tcacgccagg acatgagcta cacatcacat 12180 attagcatgc agatgcggac gatttgtcac tcacttcaaa cacctaaaag agcttctctc 12240 tcacagcaca cacacatatg catgcaatat ttacacgtga tcgccatgca aatctccatt 12300 ctcacctata aattagaggc tcggcttcac tttttactca aaccaaaact catcactaca 12360 agatccacaa ccaaaaatgg ctgcaaggaa cgtgagtgcg gctcatgaga tgcatgacga 12420 gaagaggata gctgttgtgg gcatggctgt tcagtatgct ggatgcaaaa cgaaagacga 12480 gttctgggaa gttctcatga atggaaaagt tgagagcaaa gtgatcagcg acaaacgtct 12540 tggttcaaat taccgtgctg aacactacaa agctgaaagg agcaagtatg ctgacacctt 12600 ctgcaatgag acatatggaa ctcttgatga gaacgaaata gacaatgagc atgaactcct 12660 tttgaacctt gccaaacaag ccttggctga aacatctgtc aaagattcaa caagatgtgg 12720 catagtttct ggatgcctca gcttcccgat ggacaacctc caaggtgaac tcttgaatgt 12780 gtaccagaac catgttgaga agaaacttgg tgccagagtt ttcaaagatg cctctcactg 12840 gtcagaaaga gaacagtcca acaaaccaga ggctggtgac agaagaatct tcatggaccc 12900 tgcttccttt gttgctgagg aactcaatct tggcgctctc cactactctg ttgatgctgc 12960 ctgtgccact gcgctctatg tgctcagact ggcgcaagat cacttggtct ctggtgcagc 13020 tgatgtgatg ctttgtggtg caacatgcct gccggagccg tttttcatac tctctgggtt 13080 ctcaaccttc caagccatgc ctgttggaac gggtcagaat gtgtccatgc cgctgcacaa 13140 agattctcaa ggattgaccc ctggtgaagg aggttccatc atggtcctca aacgtttgga 13200 tgacgcaatc agagatggag atcacatcta tggaacactt ttgggagcca atgtcagcaa 13260 cagtggcact ggtctgccac ttaaacccct tttgccgtca gagaaaaagt gcctcatgga 13320 cacctacaca cgtatcaatg tgcatcccca caagattcag tatgttgaat gccatgcaac 13380 tgggactcca caaggtgatc gtgtggaaat tgatgctgtg aaggcttgct ttgaaggcaa 13440 agtcccacgt tttggaacca caaaagggaa ctttggacac acattggtgg cagctgggtt 13500 tgctggcatg tgcaaagtgc ttctctccat gaagcacggg atcattccac ccactccggg 13560 aatagatgac gaaacgaaga tggaccctct ggttgtctct ggtgaggcca ttccctggcc 13620 agaaacgaat ggagaaccga agcgtgctgg tctctcagcg tttggatttg gtgggaccaa 13680 tgctcatgct gtctttgagg aacatgaccc aagcaatgct gcatgcactg gccatgactc 13740 catttctgct ctctctgcac gttgtggtgg tgagtcaaac atgagaatag caatcactgg 13800 gatggatgcc acttttggtg ctttgaaagg attggatgct tttgaaaggg ccatctacac 13860 tggtgctcat ggtgctatac cactcccaga aaagagatgg agattcctgg ggaaagacaa 13920 ggacttcctt gatctttgtg gagtcaaagc aacaccgcat ggctgttaca ttgaagatgt 13980 tgaagttgat ttccagaggc tgaggactcc aatgactcct gaagatatgc tcttgcctca 14040 acagcttctg gctgtcacca caattgatag ggccatattg gactctggca tgaagaaagg 14100 tggaaatgtg gctgtctttg tggggttggg gaccgatctt gaactctacc gtcaccgtgc 14160 aagggttgct ctcaaggaga gagtgaggcc tgaagccagc aagaaactca atgacatgat 14220 gcagtacatc aatgattgtg ggacaagcac ttcttacacc agctacatag gcaacttggt 14280 tgcaacaagg gtcagttcac aatggggctt cacgggacct tccttcacaa tcacagaagg 14340 caacaattct gtgtacagat gtgctgaact tggcaaatac ctcttggaaa cgggagaggt 14400 tgatggggtt gtggttgcgg gtgttgatct ctgtgggagt gctgagaacc tttacgtcaa 14460 gtcaaggcgt ttcaaggtgt ctacatctga caccccgaga gccagctttg atgcagctgc 14520 tgatggttac tttgttggag agggctgtgg tgcatttgtg ctcaagcgtg agacaagctg 14580 caccaaggat gacagaatct atgcttgcat ggatgccata gttcctggaa atgttccatc 14640 tgcatgtttg agagaggcat tggatcaagc cagagtcaag cctggagaca tcgagatgtt 14700 ggagctttct gctgattcag cgaggcattt gaaggacccg tctgttctcc caaaggaact 14760 cactgctgag gaagagattg gcggacttca gacgattctt cgtgacgatg acaagctccc 14820 aaggaatgtt gcaactggaa gtgtcaaggc cactgttggt gacactggtt atgcttctgg 14880 tgctgccagt ttgattaagg ctgcgctttg catctacaac cgttacctgc ccagcaatgg 14940 agatgactgg gatgaacctg cacctgaagc accctgggac agcacactct ttgcgtgcca 15000 gacctcaaga gcttggttga agaaccctgg tgagagacgt tatgcggctg tttccggagt 15060 cagtgaaacc agaagttgct acagtgtgct gctttctgaa gcagagggac actacgagag 15120 agagaacaga atcagtttgg atgaggaagc acccaagctc attgtgttga gagcagactc 15180 ccatgaagag attcttggaa gattggacaa gataagggaa aggttcttgc agcccacggg 15240 tgctgcaccc agagagtctg aattgaaggc gcaagcccgt aggatctttc ttgaactcct 15300 tggtgagacc cttgcccaag atgcagcttc atctggaagt cagaagcccc ttgctctcag 15360 cttggtctcc actccatcca agctccagag agaggtggaa cttgcggcaa aaggcattcc 15420 gagatgtctc aagatgagac gtgattggag ttcccctgct ggaagcagat atgcgcctga 15480 gcctttggcc agtgacagag ttgccttcat gtatggagaa ggaaggagcc cttactatgg 15540 catcacacaa gacattcata ggatttggcc tgaacttcat gaggtcatca atgagaaaac 15600 aaaccgtctc tgggctgaag gtgacagatg ggtcatgcca agggccagct tcaagagtga 15660 gcttgagtca caacagcaag agtttgacag aaacatgatt gaaatgttcc gtcttggaat 15720 cctcacctca attgccttca caaacctggc gagagatgtt ctcaacatca cgcccaaagc 15780 agcctttgga ctcagtttgg gagagatttc catgatcttt gctttctcca agaaaaacgg 15840 tttgataagc gatcagttga caaaggattt gagagagtca gatgtctgga acaaagctct 15900 ggctgttgag ttcaatgcgc tcagagaagc ctggggcatt ccacagtctg tcccaaaaga 15960 tgagttctgg caaggttaca tagtgagggg aacgaaacaa gacatagaag cagccatagc 16020 accagactca aagtatgtga ggttgaccat catcaatgat gccaacactg ctctcatcag 16080 tggaaagcct gatgcatgca aagctgcgat tgctcgtctt ggtggcaaca tccctgcgct 16140 tccagtcact caaggcatgt gtgggcattg cccagaggtg ggaccttaca ccaaggacat 16200 cgcaaagatc cacgccaacc ttgagttccc tgtggttgat ggtcttgatc tctggactac 16260 cataaaccag aagaggcttg tgccaagagc cacgggtgcc aaagatgaat gggctccttc 16320 atcctttggg gagtatgctg gacagctcta tgagaaacaa gccaacttcc ctcagatagt 16380 tgaaaccatc tacaagcaga actatgatgt gtttgttgaa gttgggccaa acaatcacag 16440 aagcacagca gtgagaacca cgcttggacc acagagaaac caccttgctg gtgccataga 16500 caagcagaac gaggatgctt ggacgacaat agtcaagctc gtggcttcat tgaaggctca 16560 ccttgttcct ggagtgacca tcagtccgct ctaccactcc aagctcgttg cagaggctga 16620 ggcttgttat gctgcgctct gtaaaggtga gaaacccaag aaaaacaagt ttgtgagaaa 16680 gattcagttg aatggcagat tcaacagcaa ggcagacccc atctcctcag cagaccttgc 16740 atccttccca cctgcggacc ctgcgattga agctgccatc tcttcaagga tcatgaagcc 16800 tgtggctccg aaattctatg cgcgtctcaa cattgatgag caagatgaga ccagagatcc 16860 gatcttgaac aaggacaatg cgccttccag ctccagctct tccagcagtt cctcttccag 16920 cagttcatct ccgtctcctg ctccttcagc tccagttcag aagaaagctg ccccagctgc 16980 ggagacaaaa gctgttgcca gtgctgatgc acttcgtagt gccctgcttg atcttgactc 17040 catgcttgcg ctcagctctg caagtgcttc tggcaacctt gttgagactg cgccatctga 17100 tgccagtgtc attgtccctc cgtgcaacat tgctgatctt ggcagcagag ctttcatgaa 17160 aacttatggt gtttctgcgc ctctgtacac cggtgcaatg gccaagggaa ttgcctctgc 17220 ggacttggtg atcgcagctg gaaggcaagg aatccttgcg tcatttggtg ctggaggcct 17280 tccaatgcaa gttgtgcgtg agtccattga aaagattcaa gctgccctgc ccaatggacc 17340 atatgctgtc aacctcatcc attctccttt tgacagcaac cttgagaagg gaaatgttga 17400 tctcttcttg gaaaagggtg tcacttttgt tgaggcttca gccttcatga cgttgacacc 17460 gcaagttgtc agatacagag cggctgggtt gacgaggaat gcagatgggt cagtcaacat 17520 aaggaacaga atcattggga aagtctccag aactgagctg gctgaaatgt tcatgcgtcc 17580 tgcacctgag catcttttgc agaagctcat tgcttctggg gagatcaatc aagaacaagc 17640 tgagttggcc agacgtgttc ctgtggctga tgacattgcg gttgaagctg attctggtgg 17700 acacactgac aaccgtccaa tccatgtcat tctgcctctg attatcaacc tgagggatag 17760 gcttcacaga gagtgtggat acccagcaaa cctgagggtt cgtgttggtg ctgggggtgg 17820 cattgggtgt ccccaagctg cgctggcaac tttcaacatg ggtgcctctt tcattgtcac 17880 tggaacagtg aaccaagttg ccaaacaatc tgggacgtgt gacaatgtga ggaaacagct 17940 tgcgaaagca acttactcag atgtttgcat ggcaccggca gctgacatgt ttgaggaagg 18000 tgtgaaactt caagttttga agaaaggaac aatgttccct tcaagagcca acaaactcta 18060 tgagttgttc tgcaagtatg attcttttga gtccatgcct ccagcagagc ttgcgagggt 18120 tgagaaaaga atcttcagtc gtgcattgga agaggtctgg gatgagacca agaacttcta 18180 catcaaccgt ctccacaacc cagagaagat acagagggct gagagggacc ccaaactgaa 18240 gatgtcactg tgtttcagat ggtatctgtc cctggcttca aggtgggcca acactggagc 18300 ttctgacaga gtcatggact accaagtttg gtgtggtcct gccattggtt cattcaatga 18360 tttcatcaaa ggaacttacc ttgatccggc agtggcaaat gagtacccgt gtgttgtgca 18420 gatcaacaaa cagattctta gaggagcgtg cttcttgagg cgtcttgaaa tactgagaaa 18480 tgcaaggctt tcagatggtg cagctgcact tgtggcgagc attgatgaca catatgtccc 18540 tgctgagaag ctgtgagtag ttagcttaat cacttaggtc accattccaa acgaaatatc 18600 ctcgagagtg tgtataccac ggtgatatga gtgtggttgt tgatgtatgt taacactaca 18660 tagtcatggt gtgtgttcca taaataatgt actaatgtaa taagaactac tccgtagacg 18720 gtaataaaag agaagttttt ttttttactc ttgctacttt cctataaagt gatgattaac 18780 aacagataca ccaaaaagaa aacaattaat ctatattcac aatgaagcag tactagtcta 18840 ttgaacatgt cagattttct ttttctagat gtctaatcaa gccttcaagg ctagtgataa 18900 aagatcatcc aatgggatcc aacaaagact caaatctggt tttgatcaga tacttcgaaa 18960 ctatttttgt atgcactaaa ttatgcaagt gttcttttat ttggtgaaga ctctttagaa 19020 gcaaagaacg ataagcagta ataaaaaaaa taaagttcag ttttaagatt tgttattgac 19080 ttagcggccg cttaattaaa ctagtgctag cctcgaggtc gaccaacttt tctatacaaa 19140 gttgtctaga cctaggacta ggcactgagt gactcactga cctgactgag tgactggcgg 19200 ccgcagctta tagtaaagat ttttttgtta ataaaatgaa agttaatatt tatggattta 19260 tttgaacata tttacaagtt tttgttaata aatttattta cagaagtgtt aaatttgaaa 19320 atgtgttaaa tgagagttaa tatttatgga tataatatta ctttatttaa aaataaaatg 19380 aatttatatt aaaatatgat ttggtaaaag atattataaa gaggaacttg gatgagaaag 19440 acgacgatgg gcaaaagttg catggcacac cgcttttgtt cagtaacatc tgcactgtgg 19500 cgcctttttc acactcacca tgcatgctgc cacgtcagct ttcacactat gacaccactg 19560 gccatacacg ttgccacctc agctttctcc tcttctcact atgacacgac tggccatgca 19620 tgcatgcatg ctgccacctc agctcccgcc tcttcacccg tgtctttctc tcatgtcgca 19680 gtgctgcgtg ccaacctctt ctctctataa atagagagaa gagaatgatg gttaaactca 19740 aaccaaaaca agaaaacata cacaaatagc aaaacggatc cacaaccaaa aatggcgctt 19800 cgtgtcaaga ccaacaagaa gccatgttgg gagatgacca aggaggaact gacctctggc 19860 aaaactgagg tgttcaacta tgaggaactc ctggagtttg cagagggtga catagctaag 19920 gtttttggtc cggagtttgc tgtcatagac aagtacccca gaagggtgag gctgcctgct 19980 cgtgaatacc tgcttgtgac aagggtcaca ctcatggatg ccgaggtcaa caactacaga 20040 gttggtgcca gaatggtcac tgagtatgat cttccagtga atggcgaatt gtctgaggga 20100 ggtgattgtc cctgggcagt tctggttgag agtggacagt gcgatttgat gctgatatca 20160 tacatgggaa ttgatttcca gaaccaaggt gacagagttt accgtctgct caacaccacg 20220 ttgacattct atggggttgc ccatgaggga gagacccttg agtatgacat cagagtgacc 20280 ggttttgcca aacgtttgga tggtggcatc tccatgttct tctttgagta tgactgctat 20340 gtcaatggga ggcttctcat tgagatgaga gatggctgtg ctggcttctt cacaaatgag 20400 gagcttgatg cgggaaaggg tgtggtgttc acaagagggg atttggctgc cagagctaag 20460 attccaaagc aagatgtttc tccctatgct gtcgctccct gccttcacaa aacaaaactc 20520 aacgaaaagg agatgcagac cttggttgac aaagattggg cttccgtttt tggctcaaag 20580 aatggcatgc cggagatcaa ctacaaactc tgcgcacgta agatgctcat gattgataga 20640 gtcaccagca ttgaccataa gggaggtgtg tacggtttgg gtcagttggt tggtgagaaa 20700 atcttggaga gggaccattg gtacttccca tgccattttg ttaaggatca agtcatggct 20760 ggatctcttg tcagcgacgg ttgttcacaa atgctcaaga tgtacatgat ctggctcggt 20820 ttgcacctta ccaccggacc atttgacttc agacccgtca acggacaccc gaacaaagtt 20880 aggtgtaggg gtcagatatc tcctcataag ggaaagttgg tctacgtcat ggagatcaaa 20940 gagatgggat tcgacgagga taacgatccg tacgccattg ctgacgtcaa catcattgac 21000 gttgatttcg aaaagggtca agatttcagc cttgatagaa tcagcgacta cggaaagggg 21060 gatcttaaca aaaagatcgt cgtggacttc aagggcattg ctctcaagat gcagaagaga 21120 tccacgaaca agaacccctc caaagttcag cccgtttttg ctaacggtgc agccactgtg 21180 ggaccggaag cgtccaaggc cagctctggt gccagtgcta gtgcttctgc tgcccctgcg 21240 aagcctgctt tctcagcaga tgttcttgcg ccaaagccag ttgcacttcc tgaacatatc 21300 ctcaagggtg acgctctcgc tccgaaagaa atgtcctggc atcccatgtc aaaacttcct 21360 ggcaacccga cgccgttctt ctcccctagt tcttaccctc cgagggcaat ttgcttcatc 21420 cctttccctg gcaacccact tgacaacaat tgcaaagctg gagaaatgcc tctgaactgg 21480 tacaacatgt cagagttcat gtgtggaaaa gttagcaact gcttgggacc agagttcgca 21540 agatttgata agtcaaatac gagcagaagc cctgcttttg atttggctct tgtgactaga 21600 gttgttgaag tcacaaacat ggaacatggc aagttcctga atgttgattg caatcccagc 21660 aaaggcacaa tggtggggga gtttgactgt cctcaagatg cgtggttctt tgatggttct 21720 tgcaatgatg ggcacatgcc ttattccatc atcatggaaa tcggacttca gacatccggt 21780 gttctcacca gtgtgttgaa agcaccactg acgatggaca aagatgacat tctctttagg 21840 aaccttgatg caagtgctga aatggtgagg ccagatgtgg atgtgagggg gaaaaccata 21900 aggaatgtga ccaagtgcac tggatatgca atgttgggaa agatggggat tcacagattc 21960 acgtttgagt tgtctgttga tggcgtggtt ttctacaaag gaagcacttc ctttggatgg 22020 ttcactccag aggtgtttgc tcagcaagct ggacttgaca atgggaaaaa gacggagcct 22080 tggtgcaaaa ctaacaacac gagtgtgaga agagttgaaa tcgcatcagc caaaggcaaa 22140 gaacagctga ctgagaaact tccagatgca actaatgctc aagttctcag aaggtcagaa 22200 cagtgtgagt atttggatta cctcaacatt gctcctgatt ctgggctgca tgggaaagga 22260 tatgcgcatg gacacaaaga tgttaaccca caagactggt tcttctcttg tcacttttgg 22320 tttgatcctg tgatgcctgg atctttggga attgagtcaa tgttccagct cattgaggca 22380 tttgcggtgg accagaacat accgggagaa tacaatgtga gcaatccgac ctttgcccat 22440 gcccctggaa aaacggcgtg gaagtaccgt ggtcaattga caccaaagaa ccgtgcgatg 22500 gactgtgagg tgcacatagt ttcaatcaca gcctctcctg aaaatggagg ctacgttgac 22560 atagtggcag atggagcact ttgggttgat ggtctccgtg tgtatgaagc caaagaactc 22620 agagttcgtg ttgtcagtgc aaaacctcaa gccattccgg atgttcaaca acagccaccc 22680 agcgcaaaag cggaccctgg gaaaactgga gttgcactca gtccgactca gttgagagat 22740 gtcctgcttg aagtggacaa tccattgtat cttggtgttg aaaactccaa tttggtgcag 22800 tttgaatcca aacctgcaac ttcttcaagg attgtcagta tcaaaccatg ctccataagt 22860 gatcttggtg acaagtcctt catggaaacg tacaatgtgt cagcacctct gtacactgga 22920 gcaatggcca agggaattgc gtctgcggac cttgtcattg ctgctgggaa gaggaagata 22980 ttgggttctt ttggagcggg tgggcttcca atgcaccatg tgagggcagc tttggagaag 23040 atacaagcgg ctctgcctca aggaccatat gctgtgaacc tcattcattc accttttgac 23100 agcaacttgg aaaaggggaa tgttgatctc ttcctcgaaa agggcgttac tgtggtggag 23160 gcttctgcat tcatgaccct cactccccaa gttgttcgtt accgtgcagc cggactctca 23220 aggaacgctg atgggagtgt caacatcaga aaccgtatca ttggaaaagt gtccagaaca 23280 gaactcgccg aaatgttcat cagaccggct ccggaacacc tccttgagaa actcatagct 23340 tctggtgaga taacacaaga acaagccgaa cttgcccgtc gtgttcctgt cgcagacgac 23400 atcgctgttg aagctgattc tggtggtcac actgacaacc gtcccatcca cgtcatcttg 23460 cctttgatca tcaaccttag gaacagactg cacagagagt gtggttatcc tgcgcacctt 23520 agagtcagag ttggagctgg aggcggtgtt ggctgcccac aagctgctgc ggctgccctc 23580 acaatgggag ctgccttcat agtgactggg actgtcaatc aagttgctaa acagtccggg 23640 acatgtgaca acgtgagaaa acaactcagt caagccacct acagtgacat ttgcatggct 23700 ccagcagctg atatgtttga ggaaggtgtg aaactccaag ttctcaagaa gggaacaatg 23760 ttcccatcaa gagctaacaa attgtacgaa ctcttttgca agtacgactc tttcgattca 23820 atgcctcctg ctgaacttga aaggatcgag aagaggatct tcaagagggc acttcaagag 23880 gtctgggaag agaccaagga cttttacatc aatggtttga agaatccaga gaagatacag 23940 agagcggaac atgaccccaa actcaagatg tctttgtgtt tcagatggta tcttggtctt 24000 gcatcacgtt gggccaacat gggtgctcct gatcgtgtca tggactatca agtctggtgt 24060 ggtccagcca ttggagcctt caatgacttc atcaaaggaa catatcttga tcctgctgtc 24120 tctaatgagt acccatgtgt tgtccagatc aaccttcaga ttttgcgtgg tgcatgttac 24180 ctgaggcgtc ttaatgctct gagaaatgat ccgaggattg atcttgagac tgaagatgct 24240 gcctttgtct atgagccaac gaatgcgctt tgagtagtta gcttaatcac ttaggtcacc 24300 cagtccaagc ccggcctagc acgatgatca tgagtgttgc tattgatgta tgttaatact 24360 atacacagtc atcgtgtgtg ttttataaaa ctgtaagctt aggatgtttg aggctaatgt 24420 aattagcact acaccgtaat aaatgagagg tttactttat gttttaataa tgttcttacc 24480 cttgctgctc tctaaactgt aatggcgtac ggttagctaa actaaagtaa aaatcgattc 24540 gcttgagtaa aattagtgac cgggaaagat catcgactac tactgctcag agatgtaatc 24600 ggtaattaag acttcaagct caataattaa ttaggagtaa atctggcttc gaatttttaa 24660 attgtcaact tccaaagaag gattggcaag actcaatctc aattgcccaa caaacatatg 24720 gctctttaca aatttggaat ctctttttga tggcaagaac gtcgtaattc ctctgtcgat 24780 ctcatgtaat taccaaactc atctacttat tactttcttt atatgcactt actgatgcgg 24840 ccgcttaatt aatcacgagt ggatgactgt cacgactgac tcatgactga ctactagtgc 24900 tagcctcgag gtcgacacaa ctttgtataa taaagttgtc tagtctgctt ttacatgcga 24960 gacacatctt ctaaagtaat tttaataata gttactatat tcaagatttc atatatcaaa 25020 tactcaatat tacttctaaa aaattaatta gatataatta aaatattact tttttaattt 25080 taagtttaat tgttgaattt gtgactattg atttattatt ctactatgtt taaattgttt 25140 tatagatagt ttaaagtaaa tataagtaat gtagtagagt gttagagtgt taccctaaac 25200 cataaactat aagatttatg gtggactaat tttcatatat ttcttattgc ttttaccttt 25260 tcttggtatg taagtccgta actggaatta ctgtgggttg ccatgacact ctgtggtctt 25320 ttggttcatg catggatctt gcgcaagaaa aagacaaaga acaaagaaaa aagacaaaac 25380 agagagacaa aacgcaatca cacaaccaac tcaaattagt cactggctga tcaagatcgc 25440 cgcgtccatg tatgtctaaa tgccatgcaa agcaacacgt gcttaacatg cactttaaat 25500 ggctcaccca tctcaaccca cacacaaaca cattgccttt ttcttcatca tcaccacaac 25560 cacctgtata tattcattct cttccgccac ctcaatttct tcacttcaac acacgtcaac 25620 ctgcatatgc gtgtcatccc atgcccaaat ctccatgcat gttccaacca ccttctctct 25680 tatataatac ctataaatac ctctaatatc actcacttct ttcatcatcc atccatccag 25740 agtactacta ctctactact ataatacccc aacccaactc atattcaata ctactctagg 25800 atccacaacc aaaaatgctt cagcacactt ggcttccgaa gcctcccaat ctgaccctct 25860 tgtcagatga ggttcatctc tggaggattc ctcttgacca gcctgagtca caacttcaag 25920 accttgctgc caccctgagc agtgatgaat tggcgagggc aaacagattc tactttccag 25980 aacacagaag gcgtttcact gctgggagag gcatcctcag atccatcttg ggtggatact 26040 tgggagtgga accgggtcaa gtcaagtttg attatgagtc ccgtgggaaa ccgatccttg 26100 gtgacagatt tgctgagagt ggactcctgt tcaacttgtc tcacagccag aaccttgcct 26160 tgtgtgctgt caactacacg cgtcaaatag gcattgatct tgaatatctg cgtccaacat 26220 ctgacttgga gtctcttgca aagaggttct ttctcccaag agaatatgaa ctcttgaggt 26280 cactccctga tgagcagaaa cagaagattt tctttcgtta ctggacttgc aaagaggctt 26340 atctcaaagc aacgggagat ggaatagcca aacttgaaga gatcgagata gcactcaccc 26400 caacagaacc tgccaagctc caaacagctc ctgcgtggtc tctgttggag cttgtgccag 26460 atgacaattg tgttgcagct gtggctgttg cgggttttgg ttggcagccc aagttctggc 26520 attactgagt agttagctta atcacttagg tcacctcgag tatcaaaatc tatttagaaa 26580 tacacaatat tttgttgcag gcttgctgga gaatcgatct gctatcataa aaattacaaa 26640 aaaattttat ttgcctcaat tattttagga ttggtattaa ggacgcttaa attatttgtc 26700 gggtcactac gcatcattgt gattgagaag atcagcgata cgaaatattc gtagtactat 26760 cgataattta tttgaaaatt cataagaaaa gcaaacgtta catgaattga tgaaacaata 26820 caaagacaga taaagccacg cacatttagg atattggccg agattactga atattgagta 26880 agatcacgga atttctgaca ggagcatgtc ttcaattcag cccaaatggc agttgaaata 26940 ctcaaaccgc cccatatgca ggagcggatc attcattgtt tgtttggttg cctttgccaa 27000 catgggagtc caaggttggc gcgccgaatt cgcggccgct taattaacta ctgtcactga 27060 ggccgtagac gagtacggac tgatctaact agtgctagcc tcgaggtcga cacccagctt 27120 tcttgtacaa agtggttgcg gccgcttaat taaatttaaa ttcaattaat gcaatcttga 27180 ttttcaacaa cgaaggtaat ggcgtaaaag aaaaaatgta tgttattgta ttgatctttc 27240 atgatgttga agcgtgccat aatatgatga tgtataatta aaatattaac tgtcgcattt 27300 tattgaaatg gcactgttat ttcaaccata tctttgattc tgttacatga cacgactgca 27360 agaagtaaat aatagacgcc gttgttaaag aattgctatc atatgtgcct aactagaggg 27420 aatttgagcg tcagacctaa tcaaatatta caaaatatct cactctgtcg ccagcaatgg 27480 tgtaatcagc gcagacaaat ggcgtaaaga tcgcggaaaa acctccccga gtggcatgat 27540 agctgcctct gtattgctga tttagtcagc cttatttgac ttaagggtgc cctcgttagt 27600 gacaaattgc tttcaaggag acagccatgc cccacacttt gttgaaaaac aaattgcctt 27660 tggggagacg gtaaagccag ttgctcttca ataaggaatg tcgaggaggc aatgtaaccg 27720 cctctggtag tacacttctc taatccaaaa atcaatttgt attcaagata ccgcaaaaaa 27780 cttatggttt aaaccctgca ggactagtcc agaaggtaat tatccaagat gtagcatcaa 27840 gaatccaatg tttacgggaa aaactatgga agtattatgt aagctcagca agaagcagat 27900 caatatgcgg cacatatgca acctatgttc aaaaatgaag aatgtacaga tacaagatcc 27960 tatactgcca gaatacgaag aagaatacgt agaaattgaa aaagaagaac caggcgaaga 28020 aaagaatctt gaagacgtaa gcactgacga caacaatgaa aagaagaaga taaggtcggt 28080 gattgtgaaa gagacataga ggacacatgt aaggtggaaa atgtaagggc ggaaagtaac 28140 cttatcacaa aggaatctta tcccccacta cttatccttt tatatttttc cgtgtcattt 28200 ttgcccttga gttttcctat ataaggaacc aagttcggca tttgtgaaaa caagaaaaaa 28260 tttggtgtaa gctattttct ttgaagtact gaggatacaa cttcagagaa atttgtaagt 28320 ttgtaggtac cagatctgga tcccaaacca tgtctccgga gaggagacca gttgagatta 28380 ggccagctac agcagctgat atggccgcgg tttgtgatat cgttaaccat tacattgaga 28440 cgtctacagt gaactttagg acagagccac aaacaccaca agagtggatt gatgatctag 28500 agaggttgca agatagatac ccttggttgg ttgctgaggt tgagggtgtt gtggctggta 28560 ttgcttacgc tgggccctgg aaggctagga acgcttacga ttggacagtt gagagtactg 28620 tttacgtgtc acataggcat caaaggttgg gcctaggatc tacattgtac acacatttgc 28680 ttaagtctat ggaggcgcaa ggttttaagt ctgtggttgc tgttataggc cttccaaacg 28740 atccatctgt taggttgcat gaggctttgg gatacacagc ccggggtaca ttgcgcgcag 28800 ctggatacaa gcatggtgga tggcatgatg ttggtttttg gcaaagggat tttgagttgc 28860 cagctcctcc aaggccagtt aggccagtta cccaaatctg agtagttagc ttaatcacct 28920 agagctcgat cggcggcaat agcttcttag cgccatcccg ggttgatcct atctgtgttg 28980 aaatagttgc ggtgggcaag gctctctttc agaaagacag gcggccaaag gaacccaagg 29040 tgaggtgggc tatggctctc agttccttgt ggaagcgctt ggtctaaggt gcagaggtgt 29100 tagcgggatg aagcaaaagt gtccgattgt aacaagatat gttgatccta cgtaaggata 29160 ttaaagtatg tattcatcac taatataatc agtgtattcc aatatgtact acgatttcca 29220 atgtctttat tgtcgccgta tgtaatcggc gtcacaaaat aatccccggt gactttcttt 29280 taatccagga tgaaataata tgttattata atttttgcga tttggtccgt tataggaatt 29340 gaagtgtgct tgaggtcggt cgccaccact cccatttcat aattttacat gtatttgaaa 29400 aataaaaatt tatggtattc aatttaaaca cgtatacttg taaagaatga tatcttgaaa 29460 gaaatatagt ttaaatattt attgataaaa taacaagtca ggtattatag tccaagcaaa 29520 aacataaatt tattgatgca agtttaaatt cagaaatatt tcaataactg attatatcag 29580 ctggtacatt gccgtagatg aaagactgag tgcgatatta tggtgtaata cataggaatt 29640 cgtttaaacg atctgcgtct aattttcggt ccaacttgca caggaaagac gtcgaccgcg 29700 gtagctcttg cccagcagac tgggcttcca gtcctttcgc tcgatcgggt ccaatgttgt 29760 cctcagctgt gaaccggaag cggacgacca acagtggaag aactgaaagg aacgagccgt 29820 ctataccttg atgatcggcc tctggtgaag ggtatcatcg cagccaagca agctcatgaa 29880 aggctgatgg gggaggtgta taattatgag gcccacggcg ggcttattct ttagggagga 29940 tctatctcgt tgctcaagtg catggcgcaa agcagttatt ggagtgcgga ttttcgttgg 30000 catattattc gccacgagtt agcagacgaa gagaccttca tgaacgtggc caaggccaga 30060 gttaagcaga tgttacgccc tgctgcaggc ctttctatta tccaatagtt ggttgatctt 30120 tggaaagagc ctcggctgag gcccatactg aaagagatcg atggatatcg atatgccatg 30180 ttgtttgcta gccagaacca gatcacatcc gatatgctat tgcagcttga cgcagatatg 30240 gaggataagt tgattcatgg gatcgctcag gagtagctca tccatgcacg ccgacaagaa 30300 cagaaattcc gtcgagttaa cgcagccgct tacgacggat tcgaaggtca tccattcgga 30360 atgtattagt ttgcaccagc tccgcgtcac acctgtcttc atttgaataa gatgttagca 30420 attgttttta gctttgtctt gttgtggcag ggcggcaagt gcttcagaca tcattctgtt 30480 ttcaaatttt atgctggaga acagcttctt aattcctttg gaaataatag actgcgtctt 30540 aaaattcaga tgtctggata tagatatgat tgtaaaataa cctatttaag tgtcatttag 30600 aacataagtt ttatgaatgt tcttccattt tcgtcatcga acgaataaga gtaaatacac 30660 cttttttaac attacaaata agttcttata cgttgtttat acaccgggaa tcatttccat 30720 tattttcgcg caaaagtcac ggatattcgt gaaagcgaca taaactgcga aatttgcggg 30780 gagtgtcttg agtttgcctc gaggctagcg catgcacata gacacacaca tcatctcatt 30840 gatgcttggt aataattgtc attagattgt ttttatgcat agatgcactc gaaatcagcc 30900 aattttagac aagtatcaaa cggatgtgac ttcagtacat taaaaacgtc cgcaatgtgt 30960 tattaagttg tctaagcgtc aatttgattt acaattgaat atatcctgcc ccagccagcc 31020 aacagctcga tttacaattg aatatatcct gccggccggc ccacgcgtgt cgaggaattc 31080 tgatctggcc cccatttgga cgtgaatgta gacacgtcga aataaagatt tccgaattag 31140 aataatttgt ttattgcttt cgcctataaa tacgacggat cgtaatttgt cgttttatca 31200 aaatgtactt tcattttata ataacgctgc ggacatctac atttttgaat tgaaaaaaaa 31260 ttggtaatta ctctttcttt ttctccatat tgaccatcat actcattgct gatccatgta 31320 gatttcccgg acatgaagcc atttacaatt gaatatatcc tgccgccgct gccgctttgc 31380 acccggtgga gcttgcatgt tggtttctac gcagaactga gccggttagg cagataattt 31440 ccattgagaa ctgagccatg tgcaccttcc ccccaacacg gtgagcgacg gggcaacgga 31500 gtgatccaca tgggactttt aaacatcatc cgtcggatgg cgttgcgaga gaagcagtcg 31560 atccgtgaga tcagccgacg caccgggcag gcgcgcaaca cgatcgcaaa gtatttgaac 31620 gcaggtacaa tcgagccgac gttcacgcgg aacgaccaag caagcttggc tgccattttt 31680 ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta 31740 gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca 31800 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa 31860 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc 31920 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc 31980 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg 32040 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc 32100 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc 32160 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca 32220 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct 32280 cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc 32340 agcggcgagg gcaaccagcc cggtgagcgt cggaaagggt cgacggatct tttccgctgc 32400 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg 32460 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca 32520 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc 32580 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc 32640 cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca ggaagggcag 32700 cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg aaaaggcggc 32760 ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct acaaaatcac 32820 gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg acctgggccg 32880 cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc ggttcggtga 32940 tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc ttggcaaggt 33000 catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc taaaacggcc 33060 ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag agcgacttcg 33120 cggagctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 33180 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 33240 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 33300 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 33360 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 33420 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 33480 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 33540 gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 33600 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 33660 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 33720 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 33780 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 33840 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 33900 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 33960 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 34020 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 34080 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 34140 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 34200 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 34260 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 34320 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 34380 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 34440 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 34500 ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 34560 gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 34620 tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 34680 ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 34740 gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 34800 catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 34860 tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 34920 cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 34980 tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 35040 ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 35100 cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 35160 attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 35220 accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 35280 ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 35340 cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 35400 gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 35460 agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 35520 ttccttactg ggctttctca gcccccgagc gcttagtggg aatttgtacc ccttatcgaa 35580 ccgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc 35640 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta 35700 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 35760 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 35820 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 35880 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 35940 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 36000 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 36060 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 36120 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 36180 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 36240 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 36300 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga 36360 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa 36420 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 36480 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg 36540 aaggtggttc taagcctcgt cttgcgatgg catttcgatc cattcccatt ccgcgctcaa 36600 gatggcttcc cctcggcagt tcatcagggc taaatcaatc tagccgactt gtccggtgaa 36660 atgggctgca ctccaacaga aacaatcaaa caaacataca cagcgactta ttcacacgag 36720 ctcaaattac aacggtatat atcctgccag tcagcatcat cacaccaaaa gttaggcccg 36780 aatagtttga aattagaaag ctcgcaattg aggtctacag gccaaattcg ctcttagccg 36840 tacaatatta ctcaccggat cctaaccggt ttaattaagg cgcgccatgc ccgggcaagc 36900 ggccgcacaa gtttgtacaa aaaagcaggc ttctag 36936 <210> 58 <211> 36767 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB7734 <400> 58 gatccacaac caaaaatggc agcgaggctg caagagcaga aaggaggtga gatggacacg 60 aggattgcga tcataggcat gagcgccata cttccttgtg gaaccacggt gagagagtca 120 tgggagacca taagggctgg cattgattgc ctgtctgatc ttcctgagga tcgtgttgat 180 gtgactgcgt actttgaccc agtcaagacc acaaaggaca aaatctactg caagagagga 240 ggtttcattc ctgagtatga ctttgatgcg cgtgagtttg gtctcaacat gttccagatg 300 gaagattctg atgcgaatca gaccatctca cttctcaagg tcaaagaagc acttcaagat 360 gctgggattg atgcacttgg aaaggagaag aaaaacatag gatgcgtgct tggcattgga 420 ggtggacaga agagttcaca tgagttctac agcagactca actatgtggt tgtggagaaa 480 gtcttgagaa agatggggat gccagaggaa gatgtcaaag tggctgtgga gaagtacaaa 540 gcgaacttcc cagaatggag acttgactct ttccctggat tccttgggaa tgtcactgct 600 ggcagatgca ccaacacatt caacttggat ggcatgaact gcgttgtgga tgctgcatgt 660 gcaagctcct tgatagcagt caaggttgcg attgatgaac tgctctatgg tgactgcgac 720 atgatggtca ctggtgcaac ctgcacagac aactccattg ggatgtacat ggctttctca 780 aagacacctg tgttcagcac ggacccaagt gtgagagcgt atgatgagaa aacgaaaggg 840 atgctcattg gtgagggatc agccatgttg gtgctcaaac gttatgcaga tgcagtgagg 900 gatggtgatg agattcatgc tgtgattcgt ggttgtgcaa gctcaagtga tggcaaagca 960 gctggcatct acactcccac aatcagtggt caagaggaag ccttgagacg tgcatacaat 1020 cgtgcctgcg ttgaccctgc cactgtcact ttggttgagg gtcatgggac tggcactcca 1080 gttggagata ggattgagtt gactgcgttg aggaacttgt ttgacaaagc gtatggtgaa 1140 gggaacacag agaaagttgc agttggaagt atcaagtcca gtattggtca tttgaaggct 1200 gttgctggtc ttgctggaat gatcaaggtc atcatggctc tcaaacacaa gactttgcct 1260 ggaacgatca atgtggacaa tccacccaat ctctatgaca acactcccat caatgagtca 1320 tccctctaca tcaacacgat gaacagaccc tggttccctc cacctggtgt tccgagacgt 1380 gctggaatat ccagctttgg ctttggaggt gccaactacc atgcagtgtt ggaagaggct 1440 gaaccagagc acacgacagc gtacagactc aacaaacgtc cacaaccggt gttgatgatg 1500 gctgcaactc ctgctgcgtt gcagagcctt tgtgaagctc agctgaaaga gtttgaagct 1560 gcgatcaagg agaatgagac agtcaagaat actgcctaca tcaagtgtgt caagtttgga 1620 gaacagttca agttccctgg gagcatacct gcgaccaatg cgagactggg attcttggtc 1680 aaagatgcgg aggatgcgtg ctccacgctt cgtgctatat gtgctcagtt tgccaaagat 1740 gtgaccaaag aagcgtggag gcttccgaga gaaggagttt ctttccgtgc caaaggcatt 1800 gccaccaatg gtgctgttgc tgcactcttc tctggacaag gtgctcagta cacacacatg 1860 ttcagtgaag ttgcgatgaa ctggcctcag ttcagacagt ccattgctgc aatggacgct 1920 gcacagtcca aggttgctgg aagtgacaaa gactttgaaa gagtctctca agtcctgtac 1980 ccaaggaaac cgtacgaacg tgagccagaa caagatcaca agaaaatctc actcacagca 2040 tacagccagc cgtcaaccct ggcatgtgct cttggtgcct ttgagatttt caaggaagct 2100 ggattcactc cagactttgc agctggacac agcttgggtg agtttgctgc gctctatgca 2160 gctggttgtg ttgacagaga tgagttgttt gaacttgttt gcagaagggc aaggatcatg 2220 ggaggcaaag atgcacctgc cacacccaaa ggttgcatgg cagctgtcat aggacccaac 2280 gcagagaaca tcaaggttca agctgccaac gtctggcttg gaaactcaaa ctctccgtct 2340 cagactgtca ttactgggtc tgttgaaggg attcaagctg aaagtgccag attgcagaag 2400 gaagggttca gagttgtgcc tcttgcttgt gagtctgcct tccattctcc acagatggaa 2460 aatgccagct cagctttcaa agatgtcata tccaaggttt ccttccgtac acccaaagca 2520 gaaacaaagc tcttcagcaa tgtttctgga gagacctatc cgacggatgc cagagagatg 2580 ctcactcaac acatgacctc atctgtgaag ttcctgaccc aagtgaggaa catgcaccaa 2640 gctggtgcga gaatctttgt tgagtttgga ccgaaacaag ttctcagcaa acttgtttct 2700 gaaaccctca aagatgaccc gtctgttgtc acagtgtctg tgaacccagc ttctggcacg 2760 gactctgaca tacagttgag ggatgctgca gttcagctgg ttgtggctgg tgtcaatctt 2820 caagggtttg acaagtggga tgctcctgat gccacgagga tgcaagccat caagaaaaag 2880 agaaccacac tcagactttc agctgccacc tatgtcagtg acaagaccaa gaaagtgagg 2940 gatgctgcca tgaacgacgg aagatgtgtc acatacctca agggtgctgc accactcatc 3000 aaggctcctg aacctgtggt tgacgaagct gccaagaggg aggctgaaag gcttcagaag 3060 gagcttcaag acgctcagag gcagcttgac gacgccaaac gtgcagctgc agaagccaac 3120 tccaaacttg ctgctgccaa ggaggaagcc aagactgctg cagcttcagc caagcctgca 3180 gttgacactg ctgttgtgga gaaacatcgt gccatcctca agtccatgct ggctgagctg 3240 gatggttatg gaagtgttga tgcttcttcc cttcagcaac agcaacagca acagactgct 3300 ccggcgcctg ttaaagccgc tgcccctgcc gctcccgttg caagtgctcc tgctcccgcc 3360 gtgtccaacg agttgcttga gaaagctgag acagttgtga tggaggttct cgctgccaag 3420 actggatacg aaacagacat gattgaggcg gacatggagc ttgagactga gttgggaatt 3480 gacagcatca agagggttga aattctttct gaagtccaag ctatgcttaa cgttgaggcg 3540 aaagatgttg atgctctctc taggacgaga acagtgggtg aagttgtcaa cgctatgaag 3600 gcagaaatcg ctggttcttc cgctccggcg ccggcagctg ccgcacctgc accggctaaa 3660 gcggctccag ctgcagctgc accagctgtt tctaatgagc tgctcgaaaa ggcagaaact 3720 gttgtgatgg aagtccttgc tgccaaaact ggctacgaaa ctgacatgat agaatcagac 3780 atggagcttg agacggagct tggaattgat tccatcaagc gtgtggaaat cctcagtgaa 3840 gtccaagcca tgctcaatgt ggaagctaag gatgtggatg ctctctcaag gaccagaact 3900 gtcggtgagg ttgtcaatgc catgaaggct gagatagctg gaggctccgc tcctgcgccg 3960 gccgctgccg ctcctggtcc cgctgccgcg gctccagctc cagctgcagc cgctccggcc 4020 gtctcaaacg aactccttga aaaagctgag accgtcgtga tggaggtctt ggcagctaaa 4080 accggctatg agactgacat gattgagtct gacatggaat tggagacaga acttgggata 4140 gacagcatca aacgtgttga aatcctcagt gaggtccaag ctatgctgaa cgtggaagcg 4200 aaggatgttg atgctctgtc cagaacgagg accgttggtg aggttgtgga tgccatgaag 4260 gctgaaattg ctggagggtc tgcgcccgcg ccagctgcag ccgctcctgc acccgcagcc 4320 gcggcacctg ctccagctgc acctgcacct gccgtttctt cagaactctt ggagaaggct 4380 gagacggttg tgatggaggt tttggctgca aagactggct atgagaccga catgattgaa 4440 tctgatatgg aattggagac agagcttggg atcgattcca tcaaaagagt ggagatcctg 4500 agtgaagtcc aagccatgct caatgttgaa gctaaggatg ttgatgcact ttcaagaaca 4560 aggacagtgg gtgaggttgt ggatgccatg aaggcggaga ttgctggagg gagcgcgcct 4620 gcgccagctg ccgcggcacc tgcaccggca gccgcagctc cagctccagc tgcaccagca 4680 ccggccgcgc ccgcgccagc tgtttcatcc gaactcctgg aaaaggccga gacggttgtg 4740 atggaggtgc tcgcagctaa gactggttac gagaccgaca tgattgaaag tgacatggag 4800 cttgaaaccg agttgggaat agatagcatc aaacgtgttg aaatcttgtc tgaggtccaa 4860 gctatgttga acgtggaggc aaaagatgtc gatgcgcttt caagaaccag aacagtcggt 4920 gaggtcgtgg acgccatgaa ggctgaaatt gccggaagct ctgctagtgc gccagctgca 4980 gccgcacctg cacctgctgc cgcggcacct gctccagctg cagccgctcc tgccgtcagc 5040 aatgagttgc tggagaaagc cgaaacagtt gtgatggaag tccttgctgc aaaaactggt 5100 tacgaaacgg acatgattga gtcagacatg gaattggaga ccgaacttgg aatagattcc 5160 atcaagagag tggagatcct ctctgaggtg caagccatgc tcaacgttga ggctaaagat 5220 gttgatgcac tcagtaggac aagaactgtg ggggaagttg tcgatgcgat gaaggctgag 5280 attgctggag gctctgctcc tgcgccggca gctgccgcac ctgcacccgc agccgcggca 5340 ccagctgtca gcaacgaatt gcttgagaag gctgaaacag ttgtcatgga agtgctggct 5400 gcaaaaaccg gctatgagac tgacatgatt gagtctgaca tggaacttga gacggaattg 5460 ggcattgaca gcatcaagcg tgttgagatc ctcagtgaag tccaagcaat gctcaatgtg 5520 gaggctaagg atgtggatgc cctctcaagg accagaacag ttggtgaggt cgttgatgcg 5580 atgaaggcgg agatcgctgg aagttccgcg cctgcgccag ctgcagccgc tcctgcaccc 5640 gcagccgcgg caccggctcc agctgcagcc gcaccggccg tctcctcaga actcctggaa 5700 aaggctgaga cagttgtgat ggaagtcctt gctgcgaaaa ctggctatga gaccgacatg 5760 attgaatctg acatggaact ggagaccgag cttggaattg actccatcaa acgtgttgaa 5820 atcctctctg aggttcaagc gatgttgaat gtggaggcca aagatgttga tgctctttcc 5880 agaacaagga cggtgggaga ggtggttgat gccatgaagg ctgagatagc tgggggttct 5940 gctcctgcgc cagctgcagc cgctcctgct cccgctgccg cggcaccagc tgtcagcaac 6000 gaactccttg aaaaagctga aactgttgtg atggaggttt tggctgcaaa gactggatac 6060 gagactgaca tgatagagtc agacatggag ttggaaacag agcttgggat tgatagcatc 6120 aagcgtgtgg aaatcctttc tgaggttcaa gccatgctga acgttgaggc caaagatgtc 6180 gatgctttgt caaggaccag aacggttgga gaagtggtcg atgccatgaa ggctgagata 6240 gctggtggat cagcccctgc tccggctgca gccgctcctg caagtgccgg tgctgcacca 6300 gctgtgaaga ttgattcagt tcatggtgct gactgtgacg atttgtcact gatgcacgca 6360 aaggtggttg acatcagaag gccagatgaa ctgattctgg aaagaccgga gaaccgtcca 6420 gttcttgtcg ttgacgatgg ctcagagttg acacttgctc ttgtgagggt gttgggagcc 6480 tgtgcagttg tcttgacctt tgagggattg cagttggctc agagagctgg tgcagctgcc 6540 attcgtcatg ttctggctaa ggacctttca gcagaatcag ctgagaaggc catcaaagag 6600 gctgagcaaa gatttggagc gttgggaggc ttcataagcc agcaagcaga aaggtttgaa 6660 ccagctgaga tactgggttt cacactcatg tgtgcgaagt ttgccaaagc ctctctctgc 6720 actgctgtgg ctggaggtcg tcctgccttc atcggtgtgg ctaggcttga cggcagactt 6780 ggattcacct ctcaaggcac ttctgacgca ctcaagagag cacagcgtgg agcgatcttt 6840 ggactctgca agacaatagg gttggagtgg tcagaatctg atgtgttcag ccgtggtgtg 6900 gacattgctc aagggatgca cccagaagat gctgcagttg ccatagttag ggagatggcg 6960 tgtgctgaca tcagaatcag agaggttggg attggagcaa accagcaaag atgcacaatc 7020 agagctgcca agttggagac tgggaaccct cagagacaga tagcgaaaga cgatgtgctt 7080 ctggtttctg gaggtgctcg tgggatcaca cctctttgca tccgtgagat cacgcgtcag 7140 attgctggag gcaagtacat tctgttgggt cgtagcaaag tttctgcgtc tgaaccagca 7200 tggtgtgctg gcatcactga tgagaaggct gtgcagaaag ctgccaccca agagttgaag 7260 agggccttca gtgctggtga aggacccaaa cccactccaa gggctgtcac gaaacttgtg 7320 ggatctgttc ttggtgctcg tgaagtgaga agctccattg ctgccattga agctctgggt 7380 ggcaaagcga tctacagctc ttgtgatgtg aactctgctg cagatgttgc caaagcagtt 7440 agggatgcag agtcccagct tggtgccaga gttagtggaa tcgttcacgc ttctggtgtg 7500 ctccgtgatc gtctcataga gaagaaactc cctgacgagt ttgatgctgt ctttgggacc 7560 aaagtcactg ggttggagaa tcttctggct gcagttgatc gtgccaacct caaacacatg 7620 gttctcttca gctcccttgc tggtttccac ggcaatgttg gtcagtctga ctatgcgatg 7680 gcaaacgaag ctctcaacaa gatgggtttg gaacttgcga aggatgtcag tgtcaagtcc 7740 atctgctttg gtccctggga tggtgggatg gtgacacctc aactcaagaa acagtttcaa 7800 gaaatgggag ttcagatcat tcctcgtgaa ggaggtgctg atacagttgc gaggattgtg 7860 ttgggatcaa gtcctgctga gatccttgtt ggaaactgga ggacaccatc caagaaagtt 7920 ggaagtgaca cgatcaccct gcatagaaag atcagtgcca agtccaatcc cttcttggag 7980 gatcatgtca tacaaggcag aagggtgttg ccaatgaccc ttgctattgg gagccttgct 8040 gagacctgcc ttggactctt ccctggctac tctctctggg ccatagatga cgcacagctc 8100 ttcaaaggtg tcactgttga tggagacgtc aactgtgaag tgaccctcac accgtccact 8160 gctccgagtg ggagggtgaa tgtccaagcg acgctcaaga cgttctcaag tggaaaactt 8220 gttccagcct acagagctgt catagtgttg tccaaccaag gtgctccacc tgccaatgca 8280 accatgcagc ctccgagcct tgatgcagat cctgctttgc aagggtcagt ctatgatggc 8340 aagactctct ttcatggtcc tgcgttcaga ggcattgatg acgttctcag ctgcaccaag 8400 agccagcttg ttgccaagtg ttctgctgtt cctggatcag atgcagccag aggagagttt 8460 gcaactgaca ctgatgcaca tgatcccttt gtcaatgatc ttgcctttca agcaatgttg 8520 gtctgggtga ggagaacgct gggacaagct gcactcccga actccattca gaggatagtc 8580 caacacagac cggtcccaca agacaaaccc ttctacatca ccctcagatc caatcagagt 8640 ggtggacata gccagcacaa acatgcactt cagttccaca acgaacaagg tgatctcttc 8700 attgatgttc aagcctctgt cattgcgaca gactcacttg cgttctgagt agttagctta 8760 atcacttagg tcacccagtc caagcccggc ctagcacgat gatcatgagt gttgctattg 8820 atgtatgtta atactataca cagtcatcgt gtgtgtttta taaaactgta agcttaggat 8880 gtttgaggct aatgtaatta gcactacacc gtaataaatg agaggtttac tttatgtttt 8940 aataatgttc ttacccttgc tgctctctaa actgtaatgg cgtacggtta gctaaactaa 9000 agtaaaaatc gattcgcttg agtaaaatta gtgaccggga aagatcatcg actactactg 9060 ctcagagatg taatcggtaa ttaagacttc aagctcaata attaattagg agtaaatctg 9120 gcttcgaatt tttaaattgt caacttccaa agaaggattg gcaagactca atctcaattg 9180 cccaacaaac atatggctct ttacaaattt ggaatctctt tttgatggca agaacgtcgt 9240 aattcctctg tcgatctcat gtaattacca aactcatcta cttattactt tctttatatg 9300 cacttactga tgcggccgct taattaagat gagtgatact caggactcag gactcactct 9360 gctgatcact agtgctagcc tcgaggtcga cacaactttg tatacaaaag ttgtctagga 9420 tctgctttta catgcgagac acatcttcta aagtaatttt aataatagtt actatattca 9480 agatttcata tatcaaatac tcaatattac ttctaaaaaa ttaattagat ataattaaaa 9540 tattactttt ttaattttaa gtttaattgt tgaatttgtg actattgatt tattattcta 9600 ctatgtttaa attgttttat agatagttta aagtaaatat aagtaatgta gtagagtgtt 9660 agagtgttac cctaaaccat aaactataag atttatggtg gactaatttt catatatttc 9720 ttattgcttt taccttttct tggtatgtaa gtccgtaact ggaattactg tgggttgcca 9780 tgacactctg tggtcttttg gttcatgcat ggatcttgcg caagaaaaag acaaagaaca 9840 aagaaaaaag acaaaacaga gagacaaaac gcaatcacac aaccaactca aattagtcac 9900 tggctgatca agatcgccgc gtccatgtat gtctaaatgc catgcaaagc aacacgtgct 9960 taacatgcac tttaaatggc tcacccatct caacccacac acaaacacat tgcctttttc 10020 ttcatcatca ccacaaccac ctgtatatat tcattctctt ccgccacctc aatttcttca 10080 cttcaacaca cgtcaacctg catatgcgtg tcatcccatg cccaaatctc catgcatgtt 10140 ccaaccacct tctctcttat ataataccta taaatacctc taatatcact cacttctttc 10200 atcatccatc catccagagt actactactc tactactata ataccccaac ccaactcata 10260 ttcaatacta ctctaggatc cacaaccaaa aatggctgca aggaacgtga gtgcggctca 10320 tgagatgcat gacgagaaga ggatagctgt tgtgggcatg gctgttcagt atgctggatg 10380 caaaacgaaa gacgagttct gggaagttct catgaatgga aaagttgaga gcaaagtgat 10440 cagcgacaaa cgtcttggtt caaattaccg tgctgaacac tacaaagctg aaaggagcaa 10500 gtatgctgac accttctgca atgagacata tggaactctt gatgagaacg aaatagacaa 10560 tgagcatgaa ctccttttga accttgccaa acaagccttg gctgaaacat ctgtcaaaga 10620 ttcaacaaga tgtggcatag tttctggatg cctcagcttc ccgatggaca acctccaagg 10680 tgaactcttg aatgtgtacc agaaccatgt tgagaagaaa cttggtgcca gagttttcaa 10740 agatgcctct cactggtcag aaagagaaca gtccaacaaa ccagaggctg gtgacagaag 10800 aatcttcatg gaccctgctt cctttgttgc tgaggaactc aatcttggcg ctctccacta 10860 ctctgttgat gctgcctgtg ccactgcgct ctatgtgctc agactggcgc aagatcactt 10920 ggtctctggt gcagctgatg tgatgctttg tggtgcaaca tgcctgccgg agccgttttt 10980 catactctct gggttctcaa ccttccaagc catgcctgtt ggaacgggtc agaatgtgtc 11040 catgccgctg cacaaagatt ctcaaggatt gacccctggt gaaggaggtt ccatcatggt 11100 cctcaaacgt ttggatgacg caatcagaga tggagatcac atctatggaa cacttttggg 11160 agccaatgtc agcaacagtg gcactggtct gccacttaaa ccccttttgc cgtcagagaa 11220 aaagtgcctc atggacacct acacacgtat caatgtgcat ccccacaaga ttcagtatgt 11280 tgaatgccat gcaactggga ctccacaagg tgatcgtgtg gaaattgatg ctgtgaaggc 11340 ttgctttgaa ggcaaagtcc cacgttttgg aaccacaaaa gggaactttg gacacacatt 11400 ggtggcagct gggtttgctg gcatgtgcaa agtgcttctc tccatgaagc acgggatcat 11460 tccacccact ccgggaatag atgacgaaac gaagatggac cctctggttg tctctggtga 11520 ggccattccc tggccagaaa cgaatggaga accgaagcgt gctggtctct cagcgtttgg 11580 atttggtggg accaatgctc atgctgtctt tgaggaacat gacccaagca atgctgcatg 11640 cactggccat gactccattt ctgctctctc tgcacgttgt ggtggtgagt caaacatgag 11700 aatagcaatc actgggatgg atgccacttt tggtgctttg aaaggattgg atgcttttga 11760 aagggccatc tacactggtg ctcatggtgc tataccactc ccagaaaaga gatggagatt 11820 cctggggaaa gacaaggact tccttgatct ttgtggagtc aaagcaacac cgcatggctg 11880 ttacattgaa gatgttgaag ttgatttcca gaggctgagg actccaatga ctcctgaaga 11940 tatgctcttg cctcaacagc ttctggctgt caccacaatt gatagggcca tattggactc 12000 tggcatgaag aaaggtggaa atgtggctgt ctttgtgggg ttggggaccg atcttgaact 12060 ctaccgtcac cgtgcaaggg ttgctctcaa ggagagagtg aggcctgaag ccagcaagaa 12120 actcaatgac atgatgcagt acatcaatga ttgtgggaca agcacttctt acaccagcta 12180 cataggcaac ttggttgcaa caagggtcag ttcacaatgg ggcttcacgg gaccttcctt 12240 cacaatcaca gaaggcaaca attctgtgta cagatgtgct gaacttggca aatacctctt 12300 ggaaacggga gaggttgatg gggttgtggt tgcgggtgtt gatctctgtg ggagtgctga 12360 gaacctttac gtcaagtcaa ggcgtttcaa ggtgtctaca tctgacaccc cgagagccag 12420 ctttgatgca gctgctgatg gttactttgt tggagagggc tgtggtgcat ttgtgctcaa 12480 gcgtgagaca agctgcacca aggatgacag aatctatgct tgcatggatg ccatagttcc 12540 tggaaatgtt ccatctgcat gtttgagaga ggcattggat caagccagag tcaagcctgg 12600 agacatcgag atgttggagc tttctgctga ttcagcgagg catttgaagg acccgtctgt 12660 tctcccaaag gaactcactg ctgaggaaga gattggcgga cttcagacga ttcttcgtga 12720 cgatgacaag ctcccaagga atgttgcaac tggaagtgtc aaggccactg ttggtgacac 12780 tggttatgct tctggtgctg ccagtttgat taaggctgcg ctttgcatct acaaccgtta 12840 cctgcccagc aatggagatg actgggatga acctgcacct gaagcaccct gggacagcac 12900 actctttgcg tgccagacct caagagcttg gttgaagaac cctggtgaga gacgttatgc 12960 ggctgtttcc ggagtcagtg aaaccagaag ttgctacagt gtgctgcttt ctgaagcaga 13020 gggacactac gagagagaga acagaatcag tttggatgag gaagcaccca agctcattgt 13080 gttgagagca gactcccatg aagagattct tggaagattg gacaagataa gggaaaggtt 13140 cttgcagccc acgggtgctg cacccagaga gtctgaattg aaggcgcaag cccgtaggat 13200 ctttcttgaa ctccttggtg agacccttgc ccaagatgca gcttcatctg gaagtcagaa 13260 gccccttgct ctcagcttgg tctccactcc atccaagctc cagagagagg tggaacttgc 13320 ggcaaaaggc attccgagat gtctcaagat gagacgtgat tggagttccc ctgctggaag 13380 cagatatgcg cctgagcctt tggccagtga cagagttgcc ttcatgtatg gagaaggaag 13440 gagcccttac tatggcatca cacaagacat tcataggatt tggcctgaac ttcatgaggt 13500 catcaatgag aaaacaaacc gtctctgggc tgaaggtgac agatgggtca tgccaagggc 13560 cagcttcaag agtgagcttg agtcacaaca gcaagagttt gacagaaaca tgattgaaat 13620 gttccgtctt ggaatcctca cctcaattgc cttcacaaac ctggcgagag atgttctcaa 13680 catcacgccc aaagcagcct ttggactcag tttgggagag atttccatga tctttgcttt 13740 ctccaagaaa aacggtttga taagcgatca gttgacaaag gatttgagag agtcagatgt 13800 ctggaacaaa gctctggctg ttgagttcaa tgcgctcaga gaagcctggg gcattccaca 13860 gtctgtccca aaagatgagt tctggcaagg ttacatagtg aggggaacga aacaagacat 13920 agaagcagcc atagcaccag actcaaagta tgtgaggttg accatcatca atgatgccaa 13980 cactgctctc atcagtggaa agcctgatgc atgcaaagct gcgattgctc gtcttggtgg 14040 caacatccct gcgcttccag tcactcaagg catgtgtggg cattgcccag aggtgggacc 14100 ttacaccaag gacatcgcaa agatccacgc caaccttgag ttccctgtgg ttgatggtct 14160 tgatctctgg actaccataa accagaagag gcttgtgcca agagccacgg gtgccaaaga 14220 tgaatgggct ccttcatcct ttggggagta tgctggacag ctctatgaga aacaagccaa 14280 cttccctcag atagttgaaa ccatctacaa gcagaactat gatgtgtttg ttgaagttgg 14340 gccaaacaat cacagaagca cagcagtgag aaccacgctt ggaccacaga gaaaccacct 14400 tgctggtgcc atagacaagc agaacgagga tgcttggacg acaatagtca agctcgtggc 14460 ttcattgaag gctcaccttg ttcctggagt gaccatcagt ccgctctacc actccaagct 14520 cgttgcagag gctgaggctt gttatgctgc gctctgtaaa ggtgagaaac ccaagaaaaa 14580 caagtttgtg agaaagattc agttgaatgg cagattcaac agcaaggcag accccatctc 14640 ctcagcagac cttgcatcct tcccacctgc ggaccctgcg attgaagctg ccatctcttc 14700 aaggatcatg aagcctgtgg ctccgaaatt ctatgcgcgt ctcaacattg atgagcaaga 14760 tgagaccaga gatccgatct tgaacaagga caatgcgcct tccagctcca gctcttccag 14820 cagttcctct tccagcagtt catctccgtc tcctgctcct tcagctccag ttcagaagaa 14880 agctgcccca gctgcggaga caaaagctgt tgccagtgct gatgcacttc gtagtgccct 14940 gcttgatctt gactccatgc ttgcgctcag ctctgcaagt gcttctggca accttgttga 15000 gactgcgcca tctgatgcca gtgtcattgt ccctccgtgc aacattgctg atcttggcag 15060 cagagctttc atgaaaactt atggtgtttc tgcgcctctg tacaccggtg caatggccaa 15120 gggaattgcc tctgcggact tggtgatcgc agctggaagg caaggaatcc ttgcgtcatt 15180 tggtgctgga ggccttccaa tgcaagttgt gcgtgagtcc attgaaaaga ttcaagctgc 15240 cctgcccaat ggaccatatg ctgtcaacct catccattct ccttttgaca gcaaccttga 15300 gaagggaaat gttgatctct tcttggaaaa gggtgtcact tttgttgagg cttcagcctt 15360 catgacgttg acaccgcaag ttgtcagata cagagcggct gggttgacga ggaatgcaga 15420 tgggtcagtc aacataagga acagaatcat tgggaaagtc tccagaactg agctggctga 15480 aatgttcatg cgtcctgcac ctgagcatct tttgcagaag ctcattgctt ctggggagat 15540 caatcaagaa caagctgagt tggccagacg tgttcctgtg gctgatgaca ttgcggttga 15600 agctgattct ggtggacaca ctgacaaccg tccaatccat gtcattctgc ctctgattat 15660 caacctgagg gataggcttc acagagagtg tggataccca gcaaacctga gggttcgtgt 15720 tggtgctggg ggtggcattg ggtgtcccca agctgcgctg gcaactttca acatgggtgc 15780 ctctttcatt gtcactggaa cagtgaacca agttgccaaa caatctggga cgtgtgacaa 15840 tgtgaggaaa cagcttgcga aagcaactta ctcagatgtt tgcatggcac cggcagctga 15900 catgtttgag gaaggtgtga aacttcaagt tttgaagaaa ggaacaatgt tcccttcaag 15960 agccaacaaa ctctatgagt tgttctgcaa gtatgattct tttgagtcca tgcctccagc 16020 agagcttgcg agggttgaga aaagaatctt cagtcgtgca ttggaagagg tctgggatga 16080 gaccaagaac ttctacatca accgtctcca caacccagag aagatacaga gggctgagag 16140 ggaccccaaa ctgaagatgt cactgtgttt cagatggtat ctgtccctgg cttcaaggtg 16200 ggccaacact ggagcttctg acagagtcat ggactaccaa gtttggtgtg gtcctgccat 16260 tggttcattc aatgatttca tcaaaggaac ttaccttgat ccggcagtgg caaatgagta 16320 cccgtgtgtt gtgcagatca acaaacagat tcttagagga gcgtgcttct tgaggcgtct 16380 tgaaatactg agaaatgcaa ggctttcaga tggtgcagct gcacttgtgg cgagcattga 16440 tgacacatat gtccctgctg agaagctgtg agtagttagc ttaatcactt aggtcacctc 16500 gagtatcaaa atctatttag aaatacacaa tattttgttg caggcttgct ggagaatcga 16560 tctgctatca taaaaattac aaaaaaattt tatttgcctc aattatttta ggattggtat 16620 taaggacgct taaattattt gtcgggtcac tacgcatcat tgtgattgag aagatcagcg 16680 atacgaaata ttcgtagtac tatcgataat ttatttgaaa attcataaga aaagcaaacg 16740 ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt aggatattgg 16800 ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat gtcttcaatt 16860 cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg atcattcatt 16920 gtttgtttgg ttgcctttgc caacatggga gtccaaggtt ggcgcgccga attcgcggcc 16980 gcttaattaa tctgacctga ccactagacc tgagtgagta cagatgtgct actagtgcta 17040 gcctcgaggt cgaccaactt ttctatacaa agttgtctag acctaggtta attaagcggc 17100 cgcagcttat ttctcttttc gatactctaa ttgagccgtg cgctctatct agaccaatta 17160 gaattgatgg agctctaaag gttgctggct gttttcttgt tcatatgatt aacttctaaa 17220 cttgtgtata aatattctct gaaagtgctt cttttggcat atgtaggttg ggcaaaaacg 17280 aggaagattg cttctcaatt tggaagatga tgaacagccg aagaagaaaa taagaatagg 17340 cagtcctgct actcaatgga tctcagtcta taacggtcgt cgtcccatga aacagaggta 17400 acacattttt tgcatataca ctttgatagt tcctcactaa ctgtgtaatc ttttggtaga 17460 tatcactaca atgttggaga gacaaggctg cgccagcata tacagaaggg aaatgaagat 17520 ggccttttga ttagctgtgt agcatcagca gctaatctct gggctctcat catggatgct 17580 ggaactggat tcacttctca agtttatgag ttgtcaccgg tcttcctaca caaggtaata 17640 atcagttgaa gcaattaaga atcaatctga tttgtagtaa actaagaaga acttacctta 17700 tgatttcccc gcaggactgg attatggaac aatgggaaaa gaactactat ataagctcca 17760 tagcgggttc agataacggg agctctttag ttgttatgtc aaaaggttag tgtttagtga 17820 ataataaact tattatcaaa aagtcttcat tgacttattt atatacttgt tgtgaattgg 17880 taggaactac ttattctcag cagtcataca aagtgagtga ctcatttcca ttcaagtgga 17940 taaataagaa atggaaagaa gattttcatg taacctccat gacaactgct ggtaatcgtt 18000 ggggtgtggt aatgtcgagg aactctggct tctctgatca ggtaggtttt tgtctcttat 18060 tgtctggtgt ttttattttc ccctgatagt ctaatatgat aaactctgcg ttgtgaaagg 18120 tggtggagct tgactttttg tacccaagcg atgggataca taggaggtgg gagaatgggt 18180 atagaataac atcaatggca gcaactgcgg atcaagcagc tttcatatta agcataccaa 18240 agcgtaagat ggtggatgaa actcaagaga ctctccgcac caccgccttt ccaagtactc 18300 atgtcaaggt tggtttcttt agctttgaac acagatttgg atctttttgt tttgtttcca 18360 tatacatagg acctgagagc ttttggttga attttttttt tttcaggaca aatgggcgaa 18420 gaatctgtac attgcatcaa tatgctatgg caggacagtg tgctgatgat acacacttaa 18480 gcatcatgtg ttgtgttaga aagccgaaga caattggagc gagcctcagg gtcgtcataa 18540 taccaatcaa agacgtaaaa ccagacgcag tctctttggt tgaatgtgat gaaagggatg 18600 tgtcttggta tgtatgtacg agtaacaaaa gagaagatgc aattgagtag tagaaagatt 18660 tgagagcttt ttaaagccct tcaagtgtgt gcttttatct tattgatatc atccatttgc 18720 gttgtttaat gcgtctttag atatgtttct gtttctttct cagtgtctga atatctgata 18780 agtgcaatgt gagaaagcca caccaaacca aaatattcaa atcttatatt tttaataatg 18840 tcgaatcact cggagttgcc accttctgtg ccaattgtgc tgaatctatc acactaaaaa 18900 aaacatttct tcaaggtaat gacttgtgga ctatgttctg aattctcatt aagtttttat 18960 tttttgaagt ttaagttttt accttctttt ttgaaaaata tcgttcataa gatgtcacgc 19020 caggacatga gctacacatc acatattagc atgcagatgc ggacgatttg tcactcactt 19080 caaacaccta aaagagcttc tctctcacag cacacacaca tatgcatgca atatttacac 19140 gtgatcgcca tgcaaatctc cattctcacc tataaattag aggctcggct tcacttttta 19200 ctcaaaccaa aactcatcac tacaagatcc acaaccaaaa atggcgcttc gtgtcaagac 19260 caacaagaag ccatgttggg agatgaccaa ggaggaactg acctctggca aaactgaggt 19320 gttcaactat gaggaactcc tggagtttgc agagggtgac atagctaagg tttttggtcc 19380 ggagtttgct gtcatagaca agtaccccag aagggtgagg ctgcctgctc gtgaatacct 19440 gcttgtgaca agggtcacac tcatggatgc cgaggtcaac aactacagag ttggtgccag 19500 aatggtcact gagtatgatc ttccagtgaa tggcgaattg tctgagggag gtgattgtcc 19560 ctgggcagtt ctggttgaga gtggacagtg cgatttgatg ctgatatcat acatgggaat 19620 tgatttccag aaccaaggtg acagagttta ccgtctgctc aacaccacgt tgacattcta 19680 tggggttgcc catgagggag agacccttga gtatgacatc agagtgaccg gttttgccaa 19740 acgtttggat ggtggcatct ccatgttctt ctttgagtat gactgctatg tcaatgggag 19800 gcttctcatt gagatgagag atggctgtgc tggcttcttc acaaatgagg agcttgatgc 19860 gggaaagggt gtggtgttca caagagggga tttggctgcc agagctaaga ttccaaagca 19920 agatgtttct ccctatgctg tcgctccctg ccttcacaaa acaaaactca acgaaaagga 19980 gatgcagacc ttggttgaca aagattgggc ttccgttttt ggctcaaaga atggcatgcc 20040 ggagatcaac tacaaactct gcgcacgtaa gatgctcatg attgatagag tcaccagcat 20100 tgaccataag ggaggtgtgt acggtttggg tcagttggtt ggtgagaaaa tcttggagag 20160 ggaccattgg tacttcccat gccattttgt taaggatcaa gtcatggctg gatctcttgt 20220 cagcgacggt tgttcacaaa tgctcaagat gtacatgatc tggctcggtt tgcaccttac 20280 caccggacca tttgacttca gacccgtcaa cggacacccg aacaaagtta ggtgtagggg 20340 tcagatatct cctcataagg gaaagttggt ctacgtcatg gagatcaaag agatgggatt 20400 cgacgaggat aacgatccgt acgccattgc tgacgtcaac atcattgacg ttgatttcga 20460 aaagggtcaa gatttcagcc ttgatagaat cagcgactac ggaaaggggg atcttaacaa 20520 aaagatcgtc gtggacttca agggcattgc tctcaagatg cagaagagat ccacgaacaa 20580 gaacccctcc aaagttcagc ccgtttttgc taacggtgca gccactgtgg gaccggaagc 20640 gtccaaggcc agctctggtg ccagtgctag tgcttctgct gcccctgcga agcctgcttt 20700 ctcagcagat gttcttgcgc caaagccagt tgcacttcct gaacatatcc tcaagggtga 20760 cgctctcgct ccgaaagaaa tgtcctggca tcccatgtca aaacttcctg gcaacccgac 20820 gccgttcttc tcccctagtt cttaccctcc gagggcaatt tgcttcatcc ctttccctgg 20880 caacccactt gacaacaatt gcaaagctgg agaaatgcct ctgaactggt acaacatgtc 20940 agagttcatg tgtggaaaag ttagcaactg cttgggacca gagttcgcaa gatttgataa 21000 gtcaaatacg agcagaagcc ctgcttttga tttggctctt gtgactagag ttgttgaagt 21060 cacaaacatg gaacatggca agttcctgaa tgttgattgc aatcccagca aaggcacaat 21120 ggtgggggag tttgactgtc ctcaagatgc gtggttcttt gatggttctt gcaatgatgg 21180 gcacatgcct tattccatca tcatggaaat cggacttcag acatccggtg ttctcaccag 21240 tgtgttgaaa gcaccactga cgatggacaa agatgacatt ctctttagga accttgatgc 21300 aagtgctgaa atggtgaggc cagatgtgga tgtgaggggg aaaaccataa ggaatgtgac 21360 caagtgcact ggatatgcaa tgttgggaaa gatggggatt cacagattca cgtttgagtt 21420 gtctgttgat ggcgtggttt tctacaaagg aagcacttcc tttggatggt tcactccaga 21480 ggtgtttgct cagcaagctg gacttgacaa tgggaaaaag acggagcctt ggtgcaaaac 21540 taacaacacg agtgtgagaa gagttgaaat cgcatcagcc aaaggcaaag aacagctgac 21600 tgagaaactt ccagatgcaa ctaatgctca agttctcaga aggtcagaac agtgtgagta 21660 tttggattac ctcaacattg ctcctgattc tgggctgcat gggaaaggat atgcgcatgg 21720 acacaaagat gttaacccac aagactggtt cttctcttgt cacttttggt ttgatcctgt 21780 gatgcctgga tctttgggaa ttgagtcaat gttccagctc attgaggcat ttgcggtgga 21840 ccagaacata ccgggagaat acaatgtgag caatccgacc tttgcccatg cccctggaaa 21900 aacggcgtgg aagtaccgtg gtcaattgac accaaagaac cgtgcgatgg actgtgaggt 21960 gcacatagtt tcaatcacag cctctcctga aaatggaggc tacgttgaca tagtggcaga 22020 tggagcactt tgggttgatg gtctccgtgt gtatgaagcc aaagaactca gagttcgtgt 22080 tgtcagtgca aaacctcaag ccattccgga tgttcaacaa cagccaccca gcgcaaaagc 22140 ggaccctggg aaaactggag ttgcactcag tccgactcag ttgagagatg tcctgcttga 22200 agtggacaat ccattgtatc ttggtgttga aaactccaat ttggtgcagt ttgaatccaa 22260 acctgcaact tcttcaagga ttgtcagtat caaaccatgc tccataagtg atcttggtga 22320 caagtccttc atggaaacgt acaatgtgtc agcacctctg tacactggag caatggccaa 22380 gggaattgcg tctgcggacc ttgtcattgc tgctgggaag aggaagatat tgggttcttt 22440 tggagcgggt gggcttccaa tgcaccatgt gagggcagct ttggagaaga tacaagcggc 22500 tctgcctcaa ggaccatatg ctgtgaacct cattcattca ccttttgaca gcaacttgga 22560 aaaggggaat gttgatctct tcctcgaaaa gggcgttact gtggtggagg cttctgcatt 22620 catgaccctc actccccaag ttgttcgtta ccgtgcagcc ggactctcaa ggaacgctga 22680 tgggagtgtc aacatcagaa accgtatcat tggaaaagtg tccagaacag aactcgccga 22740 aatgttcatc agaccggctc cggaacacct ccttgagaaa ctcatagctt ctggtgagat 22800 aacacaagaa caagccgaac ttgcccgtcg tgttcctgtc gcagacgaca tcgctgttga 22860 agctgattct ggtggtcaca ctgacaaccg tcccatccac gtcatcttgc ctttgatcat 22920 caaccttagg aacagactgc acagagagtg tggttatcct gcgcacctta gagtcagagt 22980 tggagctgga ggcggtgttg gctgcccaca agctgctgcg gctgccctca caatgggagc 23040 tgccttcata gtgactggga ctgtcaatca agttgctaaa cagtccggga catgtgacaa 23100 cgtgagaaaa caactcagtc aagccaccta cagtgacatt tgcatggctc cagcagctga 23160 tatgtttgag gaaggtgtga aactccaagt tctcaagaag ggaacaatgt tcccatcaag 23220 agctaacaaa ttgtacgaac tcttttgcaa gtacgactct ttcgattcaa tgcctcctgc 23280 tgaacttgaa aggatcgaga agaggatctt caagagggca cttcaagagg tctgggaaga 23340 gaccaaggac ttttacatca atggtttgaa gaatccagag aagatacaga gagcggaaca 23400 tgaccccaaa ctcaagatgt ctttgtgttt cagatggtat cttggtcttg catcacgttg 23460 ggccaacatg ggtgctcctg atcgtgtcat ggactatcaa gtctggtgtg gtccagccat 23520 tggagccttc aatgacttca tcaaaggaac atatcttgat cctgctgtct ctaatgagta 23580 cccatgtgtt gtccagatca accttcagat tttgcgtggt gcatgttacc tgaggcgtct 23640 taatgctctg agaaatgatc cgaggattga tcttgagact gaagatgctg cctttgtcta 23700 tgagccaacg aatgcgcttt gagtagttag cttaatcact taggtcacca ttccaaacga 23760 aatatcctcg agagtgtgta taccacggtg atatgagtgt ggttgttgat gtatgttaac 23820 actacatagt catggtgtgt gttccataaa taatgtacta atgtaataag aactactccg 23880 tagacggtaa taaaagagaa gttttttttt ttactcttgc tactttccta taaagtgatg 23940 attaacaaca gatacaccaa aaagaaaaca attaatctat attcacaatg aagcagtact 24000 agtctattga acatgtcaga ttttcttttt ctagatgtct aatcaagcct tcaaggctag 24060 tgataaaaga tcatccaatg ggatccaaca aagactcaaa tctggttttg atcagatact 24120 tcgaaactat ttttgtatgc actaaattat gcaagtgttc ttttatttgg tgaagactct 24180 ttagaagcaa agaacgataa gcagtaataa aaaaaataaa gttcagtttt aagatttgtt 24240 attgacttag cggccgctta attaaactag tgctagcctc gaggtcgaca caactttgta 24300 taataaagtt gtctagacct aggagctgtg ctgggtaact cacatgagtc actggtcaca 24360 tcagcggccg cagcttatag taaagatttt tttgttaata aaatgaaagt taatatttat 24420 ggatttattt gaacatattt acaagttttt gttaataaat ttatttacag aagtgttaaa 24480 tttgaaaatg tgttaaatga gagttaatat ttatggatat aatattactt tatttaaaaa 24540 taaaatgaat ttatattaaa atatgatttg gtaaaagata ttataaagag gaacttggat 24600 gagaaagacg acgatgggca aaagttgcat ggcacaccgc ttttgttcag taacatctgc 24660 actgtggcgc ctttttcaca ctcaccatgc atgctgccac gtcagctttc acactatgac 24720 accactggcc atacacgttg ccacctcagc tttctcctct tctcactatg acacgactgg 24780 ccatgcatgc atgcatgctg ccacctcagc tcccgcctct tcacccgtgt ctttctctca 24840 tgtcgcagtg ctgcgtgcca acctcttctc tctataaata gagagaagag aatgatggtt 24900 aaactcaaac caaaacaaga aaacatacac aaatagcaaa acggatccac aaccaaaaat 24960 gcttcagcac acttggcttc cgaagcctcc caatctgacc ctcttgtcag atgaggttca 25020 tctctggagg attcctcttg accagcctga gtcacaactt caagaccttg ctgccaccct 25080 gagcagtgat gaattggcga gggcaaacag attctacttt ccagaacaca gaaggcgttt 25140 cactgctggg agaggcatcc tcagatccat cttgggtgga tacttgggag tggaaccggg 25200 tcaagtcaag tttgattatg agtcccgtgg gaaaccgatc cttggtgaca gatttgctga 25260 gagtggactc ctgttcaact tgtctcacag ccagaacctt gccttgtgtg ctgtcaacta 25320 cacgcgtcaa ataggcattg atcttgaata tctgcgtcca acatctgact tggagtctct 25380 tgcaaagagg ttctttctcc caagagaata tgaactcttg aggtcactcc ctgatgagca 25440 gaaacagaag attttctttc gttactggac ttgcaaagag gcttatctca aagcaacggg 25500 agatggaata gccaaacttg aagagatcga gatagcactc accccaacag aacctgccaa 25560 gctccaaaca gctcctgcgt ggtctctgtt ggagcttgtg ccagatgaca attgtgttgc 25620 agctgtggct gttgcgggtt ttggttggca gcccaagttc tggcattact gagtagttag 25680 cttaatcact taggtcaccc agtccaagcc cggcctagca cgatgatcat gagtgttgct 25740 attgatgtat gttaatacta tacacagtca tcgtgtgtgt tttataaaac tgtaagctta 25800 ggatgtttga ggctaatgta attagcacta caccgtaata aatgagaggt ttactttatg 25860 ttttaataat gttcttaccc ttgctgctct ctaaactgta atggcgtacg gttagctaaa 25920 ctaaagtaaa aatcgattcg cttgagtaaa attagtgacc gggaaagatc atcgactact 25980 actgctcaga gatgtaatcg gtaattaaga cttcaagctc aataattaat taggagtaaa 26040 tctggcttcg aatttttaaa ttgtcaactt ccaaagaagg attggcaaga ctcaatctca 26100 attgcccaac aaacatatgg ctctttacaa atttggaatc tctttttgat ggcaagaacg 26160 tcgtaattcc tctgtcgatc tcatgtaatt accaaactca tctacttatt actttcttta 26220 tatgcactta ctgatgcggc cgcttaatta actactgtca ctgaggccgt agacgagtac 26280 ggactgatct aactagtgct agcctcgagg tcgacaccca gctttcttgt acaaagtggt 26340 tgcggccgct taattaaatt taaattcaat taatgcaatc ttgattttca acaacgaagg 26400 taatggcgta aaagaaaaaa tgtatgttat tgtattgatc tttcatgatg ttgaagcgtg 26460 ccataatatg atgatgtata attaaaatat taactgtcgc attttattga aatggcactg 26520 ttatttcaac catatctttg attctgttac atgacacgac tgcaagaagt aaataataga 26580 cgccgttgtt aaagaattgc tatcatatgt gcctaactag agggaatttg agcgtcagac 26640 ctaatcaaat attacaaaat atctcactct gtcgccagca atggtgtaat cagcgcagac 26700 aaatggcgta aagatcgcgg aaaaacctcc ccgagtggca tgatagctgc ctctgtattg 26760 ctgatttagt cagccttatt tgacttaagg gtgccctcgt tagtgacaaa ttgctttcaa 26820 ggagacagcc atgccccaca ctttgttgaa aaacaaattg cctttgggga gacggtaaag 26880 ccagttgctc ttcaataagg aatgtcgagg aggcaatgta accgcctctg gtagtacact 26940 tctctaatcc aaaaatcaat ttgtattcaa gataccgcaa aaaacttatg gtttaaaccc 27000 tgcaggacta gtccagaagg taattatcca agatgtagca tcaagaatcc aatgtttacg 27060 ggaaaaacta tggaagtatt atgtaagctc agcaagaagc agatcaatat gcggcacata 27120 tgcaacctat gttcaaaaat gaagaatgta cagatacaag atcctatact gccagaatac 27180 gaagaagaat acgtagaaat tgaaaaagaa gaaccaggcg aagaaaagaa tcttgaagac 27240 gtaagcactg acgacaacaa tgaaaagaag aagataaggt cggtgattgt gaaagagaca 27300 tagaggacac atgtaaggtg gaaaatgtaa gggcggaaag taaccttatc acaaaggaat 27360 cttatccccc actacttatc cttttatatt tttccgtgtc atttttgccc ttgagttttc 27420 ctatataagg aaccaagttc ggcatttgtg aaaacaagaa aaaatttggt gtaagctatt 27480 ttctttgaag tactgaggat acaacttcag agaaatttgt aagtttgtag gtaccagatc 27540 tggatcccaa accatgtctc cggagaggag accagttgag attaggccag ctacagcagc 27600 tgatatggcc gcggtttgtg atatcgttaa ccattacatt gagacgtcta cagtgaactt 27660 taggacagag ccacaaacac cacaagagtg gattgatgat ctagagaggt tgcaagatag 27720 atacccttgg ttggttgctg aggttgaggg tgttgtggct ggtattgctt acgctgggcc 27780 ctggaaggct aggaacgctt acgattggac agttgagagt actgtttacg tgtcacatag 27840 gcatcaaagg ttgggcctag gatctacatt gtacacacat ttgcttaagt ctatggaggc 27900 gcaaggtttt aagtctgtgg ttgctgttat aggccttcca aacgatccat ctgttaggtt 27960 gcatgaggct ttgggataca cagcccgggg tacattgcgc gcagctggat acaagcatgg 28020 tggatggcat gatgttggtt tttggcaaag ggattttgag ttgccagctc ctccaaggcc 28080 agttaggcca gttacccaaa tctgagtagt tagcttaatc acctagagct cgatcggcgg 28140 caatagcttc ttagcgccat cccgggttga tcctatctgt gttgaaatag ttgcggtggg 28200 caaggctctc tttcagaaag acaggcggcc aaaggaaccc aaggtgaggt gggctatggc 28260 tctcagttcc ttgtggaagc gcttggtcta aggtgcagag gtgttagcgg gatgaagcaa 28320 aagtgtccga ttgtaacaag atatgttgat cctacgtaag gatattaaag tatgtattca 28380 tcactaatat aatcagtgta ttccaatatg tactacgatt tccaatgtct ttattgtcgc 28440 cgtatgtaat cggcgtcaca aaataatccc cggtgacttt cttttaatcc aggatgaaat 28500 aatatgttat tataattttt gcgatttggt ccgttatagg aattgaagtg tgcttgaggt 28560 cggtcgccac cactcccatt tcataatttt acatgtattt gaaaaataaa aatttatggt 28620 attcaattta aacacgtata cttgtaaaga atgatatctt gaaagaaata tagtttaaat 28680 atttattgat aaaataacaa gtcaggtatt atagtccaag caaaaacata aatttattga 28740 tgcaagttta aattcagaaa tatttcaata actgattata tcagctggta cattgccgta 28800 gatgaaagac tgagtgcgat attatggtgt aatacatagg aattcgttta aacgatctgc 28860 gtctaatttt cggtccaact tgcacaggaa agacgtcgac cgcggtagct cttgcccagc 28920 agactgggct tccagtcctt tcgctcgatc gggtccaatg ttgtcctcag ctgtgaaccg 28980 gaagcggacg accaacagtg gaagaactga aaggaacgag ccgtctatac cttgatgatc 29040 ggcctctggt gaagggtatc atcgcagcca agcaagctca tgaaaggctg atgggggagg 29100 tgtataatta tgaggcccac ggcgggctta ttctttaggg aggatctatc tcgttgctca 29160 agtgcatggc gcaaagcagt tattggagtg cggattttcg ttggcatatt attcgccacg 29220 agttagcaga cgaagagacc ttcatgaacg tggccaaggc cagagttaag cagatgttac 29280 gccctgctgc aggcctttct attatccaat agttggttga tctttggaaa gagcctcggc 29340 tgaggcccat actgaaagag atcgatggat atcgatatgc catgttgttt gctagccaga 29400 accagatcac atccgatatg ctattgcagc ttgacgcaga tatggaggat aagttgattc 29460 atgggatcgc tcaggagtag ctcatccatg cacgccgaca agaacagaaa ttccgtcgag 29520 ttaacgcagc cgcttacgac ggattcgaag gtcatccatt cggaatgtat tagtttgcac 29580 cagctccgcg tcacacctgt cttcatttga ataagatgtt agcaattgtt tttagctttg 29640 tcttgttgtg gcagggcggc aagtgcttca gacatcattc tgttttcaaa ttttatgctg 29700 gagaacagct tcttaattcc tttggaaata atagactgcg tcttaaaatt cagatgtctg 29760 gatatagata tgattgtaaa ataacctatt taagtgtcat ttagaacata agttttatga 29820 atgttcttcc attttcgtca tcgaacgaat aagagtaaat acaccttttt taacattaca 29880 aataagttct tatacgttgt ttatacaccg ggaatcattt ccattatttt cgcgcaaaag 29940 tcacggatat tcgtgaaagc gacataaact gcgaaatttg cggggagtgt cttgagtttg 30000 cctcgaggct agcgcatgca catagacaca cacatcatct cattgatgct tggtaataat 30060 tgtcattaga ttgtttttat gcatagatgc actcgaaatc agccaatttt agacaagtat 30120 caaacggatg tgacttcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 30180 cgtcaatttg atttacaatt gaatatatcc tgccccagcc agccaacagc tcgatttaca 30240 attgaatata tcctgccggc cggcccacgc gtgtcgagga attctgatct ggcccccatt 30300 tggacgtgaa tgtagacacg tcgaaataaa gatttccgaa ttagaataat ttgtttattg 30360 ctttcgccta taaatacgac ggatcgtaat ttgtcgtttt atcaaaatgt actttcattt 30420 tataataacg ctgcggacat ctacattttt gaattgaaaa aaaattggta attactcttt 30480 ctttttctcc atattgacca tcatactcat tgctgatcca tgtagatttc ccggacatga 30540 agccatttac aattgaatat atcctgccgc cgctgccgct ttgcacccgg tggagcttgc 30600 atgttggttt ctacgcagaa ctgagccggt taggcagata atttccattg agaactgagc 30660 catgtgcacc ttccccccaa cacggtgagc gacggggcaa cggagtgatc cacatgggac 30720 ttttaaacat catccgtcgg atggcgttgc gagagaagca gtcgatccgt gagatcagcc 30780 gacgcaccgg gcaggcgcgc aacacgatcg caaagtattt gaacgcaggt acaatcgagc 30840 cgacgttcac gcggaacgac caagcaagct tggctgccat ttttggggtg aggccgttcg 30900 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 30960 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 31020 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 31080 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 31140 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 31200 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 31260 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 31320 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 31380 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 31440 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 31500 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 31560 agcccggtga gcgtcggaaa gggtcgacgg atcttttccg ctgcataacc ctgcttcggg 31620 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc 31680 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt 31740 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg 31800 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag 31860 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg 31920 tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc 31980 ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat 32040 gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg cggcctgctg 32100 aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac gatcctcgcc 32160 ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat gggcgtggtc 32220 cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg tgcgcgtgat 32280 tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc tggtattcgt 32340 gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga 32400 cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga 32460 ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg 32520 gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga 32580 tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt 32640 cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc 32700 ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg aacaggaggc 32760 ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg 32820 aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct 32880 gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg 32940 gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg 33000 caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga 33060 cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca 33120 gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta 33180 cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga 33240 atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct 33300 ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg 33360 ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa 33420 attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt 33480 cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc 33540 ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg 33600 aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta 33660 gggccttgtg gggtcagttc cggctggggg ttcagcagcc agcgctttac tggcatttca 33720 ggaacaagcg ggcactgctc gacgcacttg cttcgctcag tatcgctcgg gacgcacggc 33780 gcgctctacg aactgccgat aaacagagga ttaaaattga caattgtgat taaggctcag 33840 attcgacggc ttggagcggc cgacgtgcag gatttccgcg agatccgatt gtcggccctg 33900 aagaaagctc cagagatgtt cgggtccgtt tacgagcacg aggagaaaaa gcccatggag 33960 gcgttcgctg aacggttgcg agatgccgtg gcattcggcg cctacatcga cggcgagatc 34020 attgggctgt cggtcttcaa acaggaggac ggccccaagg acgctcacaa ggcgcatctg 34080 tccggcgttt tcgtggagcc cgaacagcga ggccgagggg tcgccggtat gctgctgcgg 34140 gcgttgccgg cgggtttatt gctcgtgatg atcgtccgac agattccaac gggaatctgg 34200 tggatgcgca tcttcatcct cggcgcactt aatatttcgc tattctggag cttgttgttt 34260 atttcggtct accgcctgcc gggcggggtc gcggcgacgg taggcgctgt gcagccgctg 34320 atggtcgtgt tcatctctgc cgctctgcta ggtagcccga tacgattgat ggcggtcctg 34380 ggggctattt gcggaactgc gggcgtggcg ctgttggtgt tgacaccaaa cgcagcgcta 34440 gatcctgtcg gcgtcgcagc gggcctggcg ggggcggttt ccatggcgtt cggaaccgtg 34500 ctgacccgca agtggcaacc tcccgtgcct ctgctcacct ttaccgcctg gcaactggcg 34560 gccggaggac ttctgctcgt tccagtagct ttagtgtttg atccgccaat cccgatgcct 34620 acaggaacca atgttctcgg cctggcgtgg ctcggcctga tcggagcggg tttaacctac 34680 ttcctttggt tccgggggat ctcgcgactc gaacctacag ttgtttcctt actgggcttt 34740 ctcagccccc gagcgcttag tgggaatttg taccccttat cgaaccggga gcacaggatg 34800 acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt caggagttaa 34860 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg 34920 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg 34980 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg 35040 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag 35100 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt 35160 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg 35220 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa 35280 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg 35340 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact 35400 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa 35460 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc 35520 agtatcagcc cgtcatactt gaagctaggc aggcttatct tggacaagaa gatcgcttgg 35580 cctcgcgcgc agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg 35640 tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta 35700 actcaagcgt tagagagctg gggaagacta tgcgcgatct gttgaaggtg gttctaagcc 35760 tcgtcttgcg atggcatttc gatccattcc cattccgcgc tcaagatggc ttcccctcgg 35820 cagttcatca gggctaaatc aatctagccg acttgtccgg tgaaatgggc tgcactccaa 35880 cagaaacaat caaacaaaca tacacagcga cttattcaca cgagctcaaa ttacaacggt 35940 atatatcctg ccagtcagca tcatcacacc aaaagttagg cccgaatagt ttgaaattag 36000 aaagctcgca attgaggtct acaggccaaa ttcgctctta gccgtacaat attactcacc 36060 ggatcctaac cggtttaatt aaggcgcgcc atgcccgggc aagcggccgc acaagtttgt 36120 acaaaaaagc aggcttctag acctaggtgg agtcatcacg cagactatct cagcatgtgc 36180 gtagcacgcg gccgcagctt atagtaaaga tttttttgtt aataaaatga aagttaatat 36240 ttatggattt atttgaacat atttacaagt ttttgttaat aaatttattt acagaagtgt 36300 taaatttgaa aatgtgttaa atgagagtta atatttatgg atataatatt actttattta 36360 aaaataaaat gaatttatat taaaatatga tttggtaaaa gatattataa agaggaactt 36420 ggatgagaaa gacgacgatg ggcaaaagtt gcatggcaca ccgcttttgt tcagtaacat 36480 ctgcactgtg gcgccttttt cacactcacc atgcatgctg ccacgtcagc tttcacacta 36540 tgacaccact ggccatacac gttgccacct cagctttctc ctcttctcac tatgacacga 36600 ctggccatgc atgcatgcat gctgccacct cagctcccgc ctcttcaccc gtgtctttct 36660 ctcatgtcgc agtgctgcgt gccaacctct tctctctata aatagagaga agagaatgat 36720 ggttaaactc aaaccaaaac aagaaaacat acacaaatag caaaacg 36767 <210> 59 <211> 35494 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB101493 <400> 59 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga 900 ggattgcgat cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat 960 gggagaccat aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg 1020 tgactgcgta ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag 1080 gtttcattcc tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg 1140 aagattctga tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg 1200 ctgggattga tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag 1260 gtggacagaa gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag 1320 tcttgagaaa gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag 1380 cgaacttccc agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg 1440 gcagatgcac caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg 1500 caagctcctt gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca 1560 tgatggtcac tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa 1620 agacacctgt gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga 1680 tgctcattgg tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg 1740 atggtgatga gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag 1800 ctggcatcta cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc 1860 gtgcctgcgt tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag 1920 ttggagatag gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag 1980 ggaacacaga gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg 2040 ttgctggtct tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg 2100 gaacgatcaa tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat 2160 ccctctacat caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg 2220 ctggaatatc cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg 2280 aaccagagca cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg 2340 ctgcaactcc tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg 2400 cgatcaagga gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag 2460 aacagttcaa gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca 2520 aagatgcgga ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg 2580 tgaccaaaga agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg 2640 ccaccaatgg tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt 2700 tcagtgaagt tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg 2760 cacagtccaa ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc 2820 caaggaaacc gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat 2880 acagccagcc gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg 2940 gattcactcc agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag 3000 ctggttgtgt tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg 3060 gaggcaaaga tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg 3120 cagagaacat caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc 3180 agactgtcat tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg 3240 aagggttcag agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa 3300 atgccagctc agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag 3360 aaacaaagct cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc 3420 tcactcaaca catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag 3480 ctggtgcgag aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg 3540 aaaccctcaa agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg 3600 actctgacat acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc 3660 aagggtttga caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga 3720 gaaccacact cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg 3780 atgctgccat gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca 3840 aggctcctga acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg 3900 agcttcaaga cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact 3960 ccaaacttgc tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag 4020 ttgacactgc tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg 4080 atggttatgg aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc 4140 cggcgcctgt taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg 4200 tgtccaacga gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga 4260 ctggatacga aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg 4320 acagcatcaa gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga 4380 aagatgttga tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg 4440 cagaaatcgc tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag 4500 cggctccagc tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg 4560 ttgtgatgga agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca 4620 tggagcttga gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag 4680 tccaagccat gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg 4740 tcggtgaggt tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg 4800 ccgctgccgc tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg 4860 tctcaaacga actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa 4920 ccggctatga gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag 4980 acagcatcaa acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga 5040 aggatgttga tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg 5100 ctgaaattgc tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg 5160 cggcacctgc tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg 5220 agacggttgt gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat 5280 ctgatatgga attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga 5340 gtgaagtcca agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa 5400 ggacagtggg tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg 5460 cgccagctgc cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac 5520 cggccgcgcc cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga 5580 tggaggtgct cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc 5640 ttgaaaccga gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag 5700 ctatgttgaa cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg 5760 aggtcgtgga cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag 5820 ccgcacctgc acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca 5880 atgagttgct ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt 5940 acgaaacgga catgattgag tcagacatgg aattggagac cgaacttgga atagattcca 6000 tcaagagagt ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg 6060 ttgatgcact cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga 6120 ttgctggagg ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac 6180 cagctgtcag caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg 6240 caaaaaccgg ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg 6300 gcattgacag catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg 6360 aggctaagga tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga 6420 tgaaggcgga gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg 6480 cagccgcggc accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa 6540 aggctgagac agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga 6600 ttgaatctga catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa 6660 tcctctctga ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca 6720 gaacaaggac ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg 6780 ctcctgcgcc agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg 6840 aactccttga aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg 6900 agactgacat gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca 6960 agcgtgtgga aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg 7020 atgctttgtc aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag 7080 ctggtggatc agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag 7140 ctgtgaagat tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa 7200 aggtggttga catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag 7260 ttcttgtcgt tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct 7320 gtgcagttgt cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca 7380 ttcgtcatgt tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg 7440 ctgagcaaag atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac 7500 cagctgagat actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca 7560 ctgctgtggc tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg 7620 gattcacctc tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg 7680 gactctgcaa gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg 7740 acattgctca agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt 7800 gtgctgacat cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca 7860 gagctgccaa gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc 7920 tggtttctgg aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga 7980 ttgctggagg caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat 8040 ggtgtgctgg catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga 8100 gggccttcag tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg 8160 gatctgttct tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg 8220 gcaaagcgat ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta 8280 gggatgcaga gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc 8340 tccgtgatcg tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca 8400 aagtcactgg gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg 8460 ttctcttcag ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg 8520 caaacgaagc tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca 8580 tctgctttgg tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag 8640 aaatgggagt tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt 8700 tgggatcaag tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg 8760 gaagtgacac gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg 8820 atcatgtcat acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg 8880 agacctgcct tggactcttc cctggctact ctctctgggc catagatgac gcacagctct 8940 tcaaaggtgt cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg 9000 ctccgagtgg gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg 9060 ttccagccta cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa 9120 ccatgcagcc tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca 9180 agactctctt tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga 9240 gccagcttgt tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg 9300 caactgacac tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg 9360 tctgggtgag gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc 9420 aacacagacc ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg 9480 gtggacatag ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca 9540 ttgatgttca agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa 9600 tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga 9660 tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg 9720 tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta 9780 ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa 9840 gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc 9900 tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg 9960 cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc 10020 ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta 10080 attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc 10140 acttactgat gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctaggat 10260 ctgcttttac atgcgagaca catcttctaa agtaatttta ataatagtta ctatattcaa 10320 gatttcatat atcaaatact caatattact tctaaaaaat taattagata taattaaaat 10380 attacttttt taattttaag tttaattgtt gaatttgtga ctattgattt attattctac 10440 tatgtttaaa ttgttttata gatagtttaa agtaaatata agtaatgtag tagagtgtta 10500 gagtgttacc ctaaaccata aactataaga tttatggtgg actaattttc atatatttct 10560 tattgctttt accttttctt ggtatgtaag tccgtaactg gaattactgt gggttgccat 10620 gacactctgt ggtcttttgg ttcatgcatg gatcttgcgc aagaaaaaga caaagaacaa 10680 agaaaaaaga caaaacagag agacaaaacg caatcacaca accaactcaa attagtcact 10740 ggctgatcaa gatcgccgcg tccatgtatg tctaaatgcc atgcaaagca acacgtgctt 10800 aacatgcact ttaaatggct cacccatctc aacccacaca caaacacatt gcctttttct 10860 tcatcatcac cacaaccacc tgtatatatt cattctcttc cgccacctca atttcttcac 10920 ttcaacacac gtcaacctgc atatgcgtgt catcccatgc ccaaatctcc atgcatgttc 10980 caaccacctt ctctcttata taatacctat aaatacctct aatatcactc acttctttca 11040 tcatccatcc atccagagta ctactactct actactataa taccccaacc caactcatat 11100 tcaatactac tctaggatcc acaaccaaaa atggctgcaa ggaacgtgag tgcggctcat 11160 gagatgcatg acgagaagag gatagctgtt gtgggcatgg ctgttcagta tgctggatgc 11220 aaaacgaaag acgagttctg ggaagttctc atgaatggaa aagttgagag caaagtgatc 11280 agcgacaaac gtcttggttc aaattaccgt gctgaacact acaaagctga aaggagcaag 11340 tatgctgaca ccttctgcaa tgagacatat ggaactcttg atgagaacga aatagacaat 11400 gagcatgaac tccttttgaa ccttgccaaa caagccttgg ctgaaacatc tgtcaaagat 11460 tcaacaagat gtggcatagt ttctggatgc ctcagcttcc cgatggacaa cctccaaggt 11520 gaactcttga atgtgtacca gaaccatgtt gagaagaaac ttggtgccag agttttcaaa 11580 gatgcctctc actggtcaga aagagaacag tccaacaaac cagaggctgg tgacagaaga 11640 atcttcatgg accctgcttc ctttgttgct gaggaactca atcttggcgc tctccactac 11700 tctgttgatg ctgcctgtgc cactgcgctc tatgtgctca gactggcgca agatcacttg 11760 gtctctggtg cagctgatgt gatgctttgt ggtgcaacat gcctgccgga gccgtttttc 11820 atactctctg ggttctcaac cttccaagcc atgcctgttg gaacgggtca gaatgtgtcc 11880 atgccgctgc acaaagattc tcaaggattg acccctggtg aaggaggttc catcatggtc 11940 ctcaaacgtt tggatgacgc aatcagagat ggagatcaca tctatggaac acttttggga 12000 gccaatgtca gcaacagtgg cactggtctg ccacttaaac cccttttgcc gtcagagaaa 12060 aagtgcctca tggacaccta cacacgtatc aatgtgcatc cccacaagat tcagtatgtt 12120 gaatgccatg caactgggac tccacaaggt gatcgtgtgg aaattgatgc tgtgaaggct 12180 tgctttgaag gcaaagtccc acgttttgga accacaaaag ggaactttgg acacacattg 12240 gtggcagctg ggtttgctgg catgtgcaaa gtgcttctct ccatgaagca cgggatcatt 12300 ccacccactc cgggaataga tgacgaaacg aagatggacc ctctggttgt ctctggtgag 12360 gccattccct ggccagaaac gaatggagaa ccgaagcgtg ctggtctctc agcgtttgga 12420 tttggtggga ccaatgctca tgctgtcttt gaggaacatg acccaagcaa tgctgcatgc 12480 actggccatg actccatttc tgctctctct gcacgttgtg gtggtgagtc aaacatgaga 12540 atagcaatca ctgggatgga tgccactttt ggtgctttga aaggattgga tgcttttgaa 12600 agggccatct acactggtgc tcatggtgct ataccactcc cagaaaagag atggagattc 12660 ctggggaaag acaaggactt ccttgatctt tgtggagtca aagcaacacc gcatggctgt 12720 tacattgaag atgttgaagt tgatttccag aggctgagga ctccaatgac tcctgaagat 12780 atgctcttgc ctcaacagct tctggctgtc accacaattg atagggccat attggactct 12840 ggcatgaaga aaggtggaaa tgtggctgtc tttgtggggt tggggaccga tcttgaactc 12900 taccgtcacc gtgcaagggt tgctctcaag gagagagtga ggcctgaagc cagcaagaaa 12960 ctcaatgaca tgatgcagta catcaatgat tgtgggacaa gcacttctta caccagctac 13020 ataggcaact tggttgcaac aagggtcagt tcacaatggg gcttcacggg accttccttc 13080 acaatcacag aaggcaacaa ttctgtgtac agatgtgctg aacttggcaa atacctcttg 13140 gaaacgggag aggttgatgg ggttgtggtt gcgggtgttg atctctgtgg gagtgctgag 13200 aacctttacg tcaagtcaag gcgtttcaag gtgtctacat ctgacacccc gagagccagc 13260 tttgatgcag ctgctgatgg ttactttgtt ggagagggct gtggtgcatt tgtgctcaag 13320 cgtgagacaa gctgcaccaa ggatgacaga atctatgctt gcatggatgc catagttcct 13380 ggaaatgttc catctgcatg tttgagagag gcattggatc aagccagagt caagcctgga 13440 gacatcgaga tgttggagct ttctgctgat tcagcgaggc atttgaagga cccgtctgtt 13500 ctcccaaagg aactcactgc tgaggaagag attggcggac ttcagacgat tcttcgtgac 13560 gatgacaagc tcccaaggaa tgttgcaact ggaagtgtca aggccactgt tggtgacact 13620 ggttatgctt ctggtgctgc cagtttgatt aaggctgcgc tttgcatcta caaccgttac 13680 ctgcccagca atggagatga ctgggatgaa cctgcacctg aagcaccctg ggacagcaca 13740 ctctttgcgt gccagacctc aagagcttgg ttgaagaacc ctggtgagag acgttatgcg 13800 gctgtttccg gagtcagtga aaccagaagt tgctacagtg tgctgctttc tgaagcagag 13860 ggacactacg agagagagaa cagaatcagt ttggatgagg aagcacccaa gctcattgtg 13920 ttgagagcag actcccatga agagattctt ggaagattgg acaagataag ggaaaggttc 13980 ttgcagccca cgggtgctgc acccagagag tctgaattga aggcgcaagc ccgtaggatc 14040 tttcttgaac tccttggtga gacccttgcc caagatgcag cttcatctgg aagtcagaag 14100 ccccttgctc tcagcttggt ctccactcca tccaagctcc agagagaggt ggaacttgcg 14160 gcaaaaggca ttccgagatg tctcaagatg agacgtgatt ggagttcccc tgctggaagc 14220 agatatgcgc ctgagccttt ggccagtgac agagttgcct tcatgtatgg agaaggaagg 14280 agcccttact atggcatcac acaagacatt cataggattt ggcctgaact tcatgaggtc 14340 atcaatgaga aaacaaaccg tctctgggct gaaggtgaca gatgggtcat gccaagggcc 14400 agcttcaaga gtgagcttga gtcacaacag caagagtttg acagaaacat gattgaaatg 14460 ttccgtcttg gaatcctcac ctcaattgcc ttcacaaacc tggcgagaga tgttctcaac 14520 atcacgccca aagcagcctt tggactcagt ttgggagaga tttccatgat ctttgctttc 14580 tccaagaaaa acggtttgat aagcgatcag ttgacaaagg atttgagaga gtcagatgtc 14640 tggaacaaag ctctggctgt tgagttcaat gcgctcagag aagcctgggg cattccacag 14700 tctgtcccaa aagatgagtt ctggcaaggt tacatagtga ggggaacgaa acaagacata 14760 gaagcagcca tagcaccaga ctcaaagtat gtgaggttga ccatcatcaa tgatgccaac 14820 actgctctca tcagtggaaa gcctgatgca tgcaaagctg cgattgctcg tcttggtggc 14880 aacatccctg cgcttccagt cactcaaggc atgtgtgggc attgcccaga ggtgggacct 14940 tacaccaagg acatcgcaaa gatccacgcc aaccttgagt tccctgtggt tgatggtctt 15000 gatctctgga ctaccataaa ccagaagagg cttgtgccaa gagccacggg tgccaaagat 15060 gaatgggctc cttcatcctt tggggagtat gctggacagc tctatgagaa acaagccaac 15120 ttccctcaga tagttgaaac catctacaag cagaactatg atgtgtttgt tgaagttggg 15180 ccaaacaatc acagaagcac agcagtgaga accacgcttg gaccacagag aaaccacctt 15240 gctggtgcca tagacaagca gaacgaggat gcttggacga caatagtcaa gctcgtggct 15300 tcattgaagg ctcaccttgt tcctggagtg accatcagtc cgctctacca ctccaagctc 15360 gttgcagagg ctgaggcttg ttatgctgcg ctctgtaaag gtgagaaacc caagaaaaac 15420 aagtttgtga gaaagattca gttgaatggc agattcaaca gcaaggcaga ccccatctcc 15480 tcagcagacc ttgcatcctt cccacctgcg gaccctgcga ttgaagctgc catctcttca 15540 aggatcatga agcctgtggc tccgaaattc tatgcgcgtc tcaacattga tgagcaagat 15600 gagaccagag atccgatctt gaacaaggac aatgcgcctt ccagctccag ctcttccagc 15660 agttcctctt ccagcagttc atctccgtct cctgctcctt cagctccagt tcagaagaaa 15720 gctgccccag ctgcggagac aaaagctgtt gccagtgctg atgcacttcg tagtgccctg 15780 cttgatcttg actccatgct tgcgctcagc tctgcaagtg cttctggcaa ccttgttgag 15840 actgcgccat ctgatgccag tgtcattgtc cctccgtgca acattgctga tcttggcagc 15900 agagctttca tgaaaactta tggtgtttct gcgcctctgt acaccggtgc aatggccaag 15960 ggaattgcct ctgcggactt ggtgatcgca gctggaaggc aaggaatcct tgcgtcattt 16020 ggtgctggag gccttccaat gcaagttgtg cgtgagtcca ttgaaaagat tcaagctgcc 16080 ctgcccaatg gaccatatgc tgtcaacctc atccattctc cttttgacag caaccttgag 16140 aagggaaatg ttgatctctt cttggaaaag ggtgtcactt ttgttgaggc ttcagccttc 16200 atgacgttga caccgcaagt tgtcagatac agagcggctg ggttgacgag gaatgcagat 16260 gggtcagtca acataaggaa cagaatcatt gggaaagtct ccagaactga gctggctgaa 16320 atgttcatgc gtcctgcacc tgagcatctt ttgcagaagc tcattgcttc tggggagatc 16380 aatcaagaac aagctgagtt ggccagacgt gttcctgtgg ctgatgacat tgcggttgaa 16440 gctgattctg gtggacacac tgacaaccgt ccaatccatg tcattctgcc tctgattatc 16500 aacctgaggg ataggcttca cagagagtgt ggatacccag caaacctgag ggttcgtgtt 16560 ggtgctgggg gtggcattgg gtgtccccaa gctgcgctgg caactttcaa catgggtgcc 16620 tctttcattg tcactggaac agtgaaccaa gttgccaaac aatctgggac gtgtgacaat 16680 gtgaggaaac agcttgcgaa agcaacttac tcagatgttt gcatggcacc ggcagctgac 16740 atgtttgagg aaggtgtgaa acttcaagtt ttgaagaaag gaacaatgtt cccttcaaga 16800 gccaacaaac tctatgagtt gttctgcaag tatgattctt ttgagtccat gcctccagca 16860 gagcttgcga gggttgagaa aagaatcttc agtcgtgcat tggaagaggt ctgggatgag 16920 accaagaact tctacatcaa ccgtctccac aacccagaga agatacagag ggctgagagg 16980 gaccccaaac tgaagatgtc actgtgtttc agatggtatc tgtccctggc ttcaaggtgg 17040 gccaacactg gagcttctga cagagtcatg gactaccaag tttggtgtgg tcctgccatt 17100 ggttcattca atgatttcat caaaggaact taccttgatc cggcagtggc aaatgagtac 17160 ccgtgtgttg tgcagatcaa caaacagatt cttagaggag cgtgcttctt gaggcgtctt 17220 gaaatactga gaaatgcaag gctttcagat ggtgcagctg cacttgtggc gagcattgat 17280 gacacatatg tccctgctga gaagctgtga gtagttagct taatcactta ggtcacctcg 17340 agtatcaaaa tctatttaga aatacacaat attttgttgc aggcttgctg gagaatcgat 17400 ctgctatcat aaaaattaca aaaaaatttt atttgcctca attattttag gattggtatt 17460 aaggacgctt aaattatttg tcgggtcact acgcatcatt gtgattgaga agatcagcga 17520 tacgaaatat tcgtagtact atcgataatt tatttgaaaa ttcataagaa aagcaaacgt 17580 tacatgaatt gatgaaacaa tacaaagaca gataaagcca cgcacattta ggatattggc 17640 cgagattact gaatattgag taagatcacg gaatttctga caggagcatg tcttcaattc 17700 agcccaaatg gcagttgaaa tactcaaacc gccccatatg caggagcgga tcattcattg 17760 tttgtttggt tgcctttgcc aacatgggag tccaaggttg gcgcgccgaa ttcgcggccg 17820 cttaattaat ctgacctgac cactagacct gagtgagtac agatgtgcta ctagtgctag 17880 cctcgaggtc gaccaacttt tctatacaaa gttgtctaga cctaggacta ggcactgagt 17940 gactcactga cctgactgag tgactggcgg ccgcagctta tagtaaagat ttttttgtta 18000 ataaaatgaa agttaatatt tatggattta tttgaacata tttacaagtt tttgttaata 18060 aatttattta cagaagtgtt aaatttgaaa atgtgttaaa tgagagttaa tatttatgga 18120 tataatatta ctttatttaa aaataaaatg aatttatatt aaaatatgat ttggtaaaag 18180 atattataaa gaggaacttg gatgagaaag acgacgatgg gcaaaagttg catggcacac 18240 cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc acactcacca tgcatgctgc 18300 cacgtcagct ttcacactat gacaccactg gccatacacg ttgccacctc agctttctcc 18360 tcttctcact atgacacgac tggccatgca tgcatgcatg ctgccacctc agctcccgcc 18420 tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg ccaacctctt ctctctataa 18480 atagagagaa gagaatgatg gttaaactca aaccaaaaca agaaaacata cacaaatagc 18540 aaaacggatc cacaaccaaa aatggcgctt cgtgtcaaga ccaacaagaa gccatgttgg 18600 gagatgacca aggaggaact gacctctggc aaaactgagg tgttcaacta tgaggaactc 18660 ctggagtttg cagagggtga catagctaag gtttttggtc cggagtttgc tgtcatagac 18720 aagtacccca gaagggtgag gctgcctgct cgtgaatacc tgcttgtgac aagggtcaca 18780 ctcatggatg ccgaggtcaa caactacaga gttggtgcca gaatggtcac tgagtatgat 18840 cttccagtga atggcgaatt gtctgaggga ggtgattgtc cctgggcagt tctggttgag 18900 agtggacagt gcgatttgat gctgatatca tacatgggaa ttgatttcca gaaccaaggt 18960 gacagagttt accgtctgct caacaccacg ttgacattct atggggttgc ccatgaggga 19020 gagacccttg agtatgacat cagagtgacc ggttttgcca aacgtttgga tggtggcatc 19080 tccatgttct tctttgagta tgactgctat gtcaatggga ggcttctcat tgagatgaga 19140 gatggctgtg ctggcttctt cacaaatgag gagcttgatg cgggaaaggg tgtggtgttc 19200 acaagagggg atttggctgc cagagctaag attccaaagc aagatgtttc tccctatgct 19260 gtcgctccct gccttcacaa aacaaaactc aacgaaaagg agatgcagac cttggttgac 19320 aaagattggg cttccgtttt tggctcaaag aatggcatgc cggagatcaa ctacaaactc 19380 tgcgcacgta agatgctcat gattgataga gtcaccagca ttgaccataa gggaggtgtg 19440 tacggtttgg gtcagttggt tggtgagaaa atcttggaga gggaccattg gtacttccca 19500 tgccattttg ttaaggatca agtcatggct ggatctcttg tcagcgacgg ttgttcacaa 19560 atgctcaaga tgtacatgat ctggctcggt ttgcacctta ccaccggacc atttgacttc 19620 agacccgtca acggacaccc gaacaaagtt aggtgtaggg gtcagatatc tcctcataag 19680 ggaaagttgg tctacgtcat ggagatcaaa gagatgggat tcgacgagga taacgatccg 19740 tacgccattg ctgacgtcaa catcattgac gttgatttcg aaaagggtca agatttcagc 19800 cttgatagaa tcagcgacta cggaaagggg gatcttaaca aaaagatcgt cgtggacttc 19860 aagggcattg ctctcaagat gcagaagaga tccacgaaca agaacccctc caaagttcag 19920 cccgtttttg ctaacggtgc agccactgtg ggaccggaag cgtccaaggc cagctctggt 19980 gccagtgcta gtgcttctgc tgcccctgcg aagcctgctt tctcagcaga tgttcttgcg 20040 ccaaagccag ttgcacttcc tgaacatatc ctcaagggtg acgctctcgc tccgaaagaa 20100 atgtcctggc atcccatgtc aaaacttcct ggcaacccga cgccgttctt ctcccctagt 20160 tcttaccctc cgagggcaat ttgcttcatc cctttccctg gcaacccact tgacaacaat 20220 tgcaaagctg gagaaatgcc tctgaactgg tacaacatgt cagagttcat gtgtggaaaa 20280 gttagcaact gcttgggacc agagttcgca agatttgata agtcaaatac gagcagaagc 20340 cctgcttttg atttggctct tgtgactaga gttgttgaag tcacaaacat ggaacatggc 20400 aagttcctga atgttgattg caatcccagc aaaggcacaa tggtggggga gtttgactgt 20460 cctcaagatg cgtggttctt tgatggttct tgcaatgatg ggcacatgcc ttattccatc 20520 atcatggaaa tcggacttca gacatccggt gttctcacca gtgtgttgaa agcaccactg 20580 acgatggaca aagatgacat tctctttagg aaccttgatg caagtgctga aatggtgagg 20640 ccagatgtgg atgtgagggg gaaaaccata aggaatgtga ccaagtgcac tggatatgca 20700 atgttgggaa agatggggat tcacagattc acgtttgagt tgtctgttga tggcgtggtt 20760 ttctacaaag gaagcacttc ctttggatgg ttcactccag aggtgtttgc tcagcaagct 20820 ggacttgaca atgggaaaaa gacggagcct tggtgcaaaa ctaacaacac gagtgtgaga 20880 agagttgaaa tcgcatcagc caaaggcaaa gaacagctga ctgagaaact tccagatgca 20940 actaatgctc aagttctcag aaggtcagaa cagtgtgagt atttggatta cctcaacatt 21000 gctcctgatt ctgggctgca tgggaaagga tatgcgcatg gacacaaaga tgttaaccca 21060 caagactggt tcttctcttg tcacttttgg tttgatcctg tgatgcctgg atctttggga 21120 attgagtcaa tgttccagct cattgaggca tttgcggtgg accagaacat accgggagaa 21180 tacaatgtga gcaatccgac ctttgcccat gcccctggaa aaacggcgtg gaagtaccgt 21240 ggtcaattga caccaaagaa ccgtgcgatg gactgtgagg tgcacatagt ttcaatcaca 21300 gcctctcctg aaaatggagg ctacgttgac atagtggcag atggagcact ttgggttgat 21360 ggtctccgtg tgtatgaagc caaagaactc agagttcgtg ttgtcagtgc aaaacctcaa 21420 gccattccgg atgttcaaca acagccaccc agcgcaaaag cggaccctgg gaaaactgga 21480 gttgcactca gtccgactca gttgagagat gtcctgcttg aagtggacaa tccattgtat 21540 cttggtgttg aaaactccaa tttggtgcag tttgaatcca aacctgcaac ttcttcaagg 21600 attgtcagta tcaaaccatg ctccataagt gatcttggtg acaagtcctt catggaaacg 21660 tacaatgtgt cagcacctct gtacactgga gcaatggcca agggaattgc gtctgcggac 21720 cttgtcattg ctgctgggaa gaggaagata ttgggttctt ttggagcggg tgggcttcca 21780 atgcaccatg tgagggcagc tttggagaag atacaagcgg ctctgcctca aggaccatat 21840 gctgtgaacc tcattcattc accttttgac agcaacttgg aaaaggggaa tgttgatctc 21900 ttcctcgaaa agggcgttac tgtggtggag gcttctgcat tcatgaccct cactccccaa 21960 gttgttcgtt accgtgcagc cggactctca aggaacgctg atgggagtgt caacatcaga 22020 aaccgtatca ttggaaaagt gtccagaaca gaactcgccg aaatgttcat cagaccggct 22080 ccggaacacc tccttgagaa actcatagct tctggtgaga taacacaaga acaagccgaa 22140 cttgcccgtc gtgttcctgt cgcagacgac atcgctgttg aagctgattc tggtggtcac 22200 actgacaacc gtcccatcca cgtcatcttg cctttgatca tcaaccttag gaacagactg 22260 cacagagagt gtggttatcc tgcgcacctt agagtcagag ttggagctgg aggcggtgtt 22320 ggctgcccac aagctgctgc ggctgccctc acaatgggag ctgccttcat agtgactggg 22380 actgtcaatc aagttgctaa acagtccggg acatgtgaca acgtgagaaa acaactcagt 22440 caagccacct acagtgacat ttgcatggct ccagcagctg atatgtttga ggaaggtgtg 22500 aaactccaag ttctcaagaa gggaacaatg ttcccatcaa gagctaacaa attgtacgaa 22560 ctcttttgca agtacgactc tttcgattca atgcctcctg ctgaacttga aaggatcgag 22620 aagaggatct tcaagagggc acttcaagag gtctgggaag agaccaagga cttttacatc 22680 aatggtttga agaatccaga gaagatacag agagcggaac atgaccccaa actcaagatg 22740 tctttgtgtt tcagatggta tcttggtctt gcatcacgtt gggccaacat gggtgctcct 22800 gatcgtgtca tggactatca agtctggtgt ggtccagcca ttggagcctt caatgacttc 22860 atcaaaggaa catatcttga tcctgctgtc tctaatgagt acccatgtgt tgtccagatc 22920 aaccttcaga ttttgcgtgg tgcatgttac ctgaggcgtc ttaatgctct gagaaatgat 22980 ccgaggattg atcttgagac tgaagatgct gcctttgtct atgagccaac gaatgcgctt 23040 tgagtagtta gcttaatcac ttaggtcacc cagtccaagc ccggcctagc acgatgatca 23100 tgagtgttgc tattgatgta tgttaatact atacacagtc atcgtgtgtg ttttataaaa 23160 ctgtaagctt aggatgtttg aggctaatgt aattagcact acaccgtaat aaatgagagg 23220 tttactttat gttttaataa tgttcttacc cttgctgctc tctaaactgt aatggcgtac 23280 ggttagctaa actaaagtaa aaatcgattc gcttgagtaa aattagtgac cgggaaagat 23340 catcgactac tactgctcag agatgtaatc ggtaattaag acttcaagct caataattaa 23400 ttaggagtaa atctggcttc gaatttttaa attgtcaact tccaaagaag gattggcaag 23460 actcaatctc aattgcccaa caaacatatg gctctttaca aatttggaat ctctttttga 23520 tggcaagaac gtcgtaattc ctctgtcgat ctcatgtaat taccaaactc atctacttat 23580 tactttcttt atatgcactt actgatgcgg ccgcttaatt aatcacgagt ggatgactgt 23640 cacgactgac tcatgactga ctactagtgc tagcctcgag gtcgacacaa ctttgtataa 23700 taaagttgtc tagtctgctt ttacatgcga gacacatctt ctaaagtaat tttaataata 23760 gttactatat tcaagatttc atatatcaaa tactcaatat tacttctaaa aaattaatta 23820 gatataatta aaatattact tttttaattt taagtttaat tgttgaattt gtgactattg 23880 atttattatt ctactatgtt taaattgttt tatagatagt ttaaagtaaa tataagtaat 23940 gtagtagagt gttagagtgt taccctaaac cataaactat aagatttatg gtggactaat 24000 tttcatatat ttcttattgc ttttaccttt tcttggtatg taagtccgta actggaatta 24060 ctgtgggttg ccatgacact ctgtggtctt ttggttcatg catggatctt gcgcaagaaa 24120 aagacaaaga acaaagaaaa aagacaaaac agagagacaa aacgcaatca cacaaccaac 24180 tcaaattagt cactggctga tcaagatcgc cgcgtccatg tatgtctaaa tgccatgcaa 24240 agcaacacgt gcttaacatg cactttaaat ggctcaccca tctcaaccca cacacaaaca 24300 cattgccttt ttcttcatca tcaccacaac cacctgtata tattcattct cttccgccac 24360 ctcaatttct tcacttcaac acacgtcaac ctgcatatgc gtgtcatccc atgcccaaat 24420 ctccatgcat gttccaacca ccttctctct tatataatac ctataaatac ctctaatatc 24480 actcacttct ttcatcatcc atccatccag agtactacta ctctactact ataatacccc 24540 aacccaactc atattcaata ctactctagg atccacaacc aaaaatgctt cagcacactt 24600 ggcttccgaa gcctcccaat ctgaccctct tgtcagatga ggttcatctc tggaggattc 24660 ctcttgacca gcctgagtca caacttcaag accttgctgc caccctgagc agtgatgaat 24720 tggcgagggc aaacagattc tactttccag aacacagaag gcgtttcact gctgggagag 24780 gcatcctcag atccatcttg ggtggatact tgggagtgga accgggtcaa gtcaagtttg 24840 attatgagtc ccgtgggaaa ccgatccttg gtgacagatt tgctgagagt ggactcctgt 24900 tcaacttgtc tcacagccag aaccttgcct tgtgtgctgt caactacacg cgtcaaatag 24960 gcattgatct tgaatatctg cgtccaacat ctgacttgga gtctcttgca aagaggttct 25020 ttctcccaag agaatatgaa ctcttgaggt cactccctga tgagcagaaa cagaagattt 25080 tctttcgtta ctggacttgc aaagaggctt atctcaaagc aacgggagat ggaatagcca 25140 aacttgaaga gatcgagata gcactcaccc caacagaacc tgccaagctc caaacagctc 25200 ctgcgtggtc tctgttggag cttgtgccag atgacaattg tgttgcagct gtggctgttg 25260 cgggttttgg ttggcagccc aagttctggc attactgagt agttagctta atcacttagg 25320 tcacctcgag tatcaaaatc tatttagaaa tacacaatat tttgttgcag gcttgctgga 25380 gaatcgatct gctatcataa aaattacaaa aaaattttat ttgcctcaat tattttagga 25440 ttggtattaa ggacgcttaa attatttgtc gggtcactac gcatcattgt gattgagaag 25500 atcagcgata cgaaatattc gtagtactat cgataattta tttgaaaatt cataagaaaa 25560 gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg cacatttagg 25620 atattggccg agattactga atattgagta agatcacgga atttctgaca ggagcatgtc 25680 ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca ggagcggatc 25740 attcattgtt tgtttggttg cctttgccaa catgggagtc caaggttggc gcgccgaatt 25800 cgcggccgct taattaacta ctgtcactga ggccgtagac gagtacggac tgatctaact 25860 agtgctagcc tcgaggtcga cacccagctt tcttgtacaa agtggttgcg gccgcttaat 25920 taaatttaaa ttcaattaat gcaatcttga ttttcaacaa cgaaggtaat ggcgtaaaag 25980 aaaaaatgta tgttattgta ttgatctttc atgatgttga agcgtgccat aatatgatga 26040 tgtataatta aaatattaac tgtcgcattt tattgaaatg gcactgttat ttcaaccata 26100 tctttgattc tgttacatga cacgactgca agaagtaaat aatagacgcc gttgttaaag 26160 aattgctatc atatgtgcct aactagaggg aatttgagcg tcagacctaa tcaaatatta 26220 caaaatatct cactctgtcg ccagcaatgg tgtaatcagc gcagacaaat ggcgtaaaga 26280 tcgcggaaaa acctccccga gtggcatgat agctgcctct gtattgctga tttagtcagc 26340 cttatttgac ttaagggtgc cctcgttagt gacaaattgc tttcaaggag acagccatgc 26400 cccacacttt gttgaaaaac aaattgcctt tggggagacg gtaaagccag ttgctcttca 26460 ataaggaatg tcgaggaggc aatgtaaccg cctctggtag tacacttctc taatccaaaa 26520 atcaatttgt attcaagata ccgcaaaaaa cttatggttt aaaccctgca ggactagtcc 26580 agaaggtaat tatccaagat gtagcatcaa gaatccaatg tttacgggaa aaactatgga 26640 agtattatgt aagctcagca agaagcagat caatatgcgg cacatatgca acctatgttc 26700 aaaaatgaag aatgtacaga tacaagatcc tatactgcca gaatacgaag aagaatacgt 26760 agaaattgaa aaagaagaac caggcgaaga aaagaatctt gaagacgtaa gcactgacga 26820 caacaatgaa aagaagaaga taaggtcggt gattgtgaaa gagacataga ggacacatgt 26880 aaggtggaaa atgtaagggc ggaaagtaac cttatcacaa aggaatctta tcccccacta 26940 cttatccttt tatatttttc cgtgtcattt ttgcccttga gttttcctat ataaggaacc 27000 aagttcggca tttgtgaaaa caagaaaaaa tttggtgtaa gctattttct ttgaagtact 27060 gaggatacaa cttcagagaa atttgtaagt ttgtaggtac cagatctgga tcccaaacca 27120 tgtctccgga gaggagacca gttgagatta ggccagctac agcagctgat atggccgcgg 27180 tttgtgatat cgttaaccat tacattgaga cgtctacagt gaactttagg acagagccac 27240 aaacaccaca agagtggatt gatgatctag agaggttgca agatagatac ccttggttgg 27300 ttgctgaggt tgagggtgtt gtggctggta ttgcttacgc tgggccctgg aaggctagga 27360 acgcttacga ttggacagtt gagagtactg tttacgtgtc acataggcat caaaggttgg 27420 gcctaggatc tacattgtac acacatttgc ttaagtctat ggaggcgcaa ggttttaagt 27480 ctgtggttgc tgttataggc cttccaaacg atccatctgt taggttgcat gaggctttgg 27540 gatacacagc ccggggtaca ttgcgcgcag ctggatacaa gcatggtgga tggcatgatg 27600 ttggtttttg gcaaagggat tttgagttgc cagctcctcc aaggccagtt aggccagtta 27660 cccaaatctg agtagttagc ttaatcacct agagctcgat cggcggcaat agcttcttag 27720 cgccatcccg ggttgatcct atctgtgttg aaatagttgc ggtgggcaag gctctctttc 27780 agaaagacag gcggccaaag gaacccaagg tgaggtgggc tatggctctc agttccttgt 27840 ggaagcgctt ggtctaaggt gcagaggtgt tagcgggatg aagcaaaagt gtccgattgt 27900 aacaagatat gttgatccta cgtaaggata ttaaagtatg tattcatcac taatataatc 27960 agtgtattcc aatatgtact acgatttcca atgtctttat tgtcgccgta tgtaatcggc 28020 gtcacaaaat aatccccggt gactttcttt taatccagga tgaaataata tgttattata 28080 atttttgcga tttggtccgt tataggaatt gaagtgtgct tgaggtcggt cgccaccact 28140 cccatttcat aattttacat gtatttgaaa aataaaaatt tatggtattc aatttaaaca 28200 cgtatacttg taaagaatga tatcttgaaa gaaatatagt ttaaatattt attgataaaa 28260 taacaagtca ggtattatag tccaagcaaa aacataaatt tattgatgca agtttaaatt 28320 cagaaatatt tcaataactg attatatcag ctggtacatt gccgtagatg aaagactgag 28380 tgcgatatta tggtgtaata cataggaatt cgtttaaacg atctgcgtct aattttcggt 28440 ccaacttgca caggaaagac gtcgaccgcg gtagctcttg cccagcagac tgggcttcca 28500 gtcctttcgc tcgatcgggt ccaatgttgt cctcagctgt gaaccggaag cggacgacca 28560 acagtggaag aactgaaagg aacgagccgt ctataccttg atgatcggcc tctggtgaag 28620 ggtatcatcg cagccaagca agctcatgaa aggctgatgg gggaggtgta taattatgag 28680 gcccacggcg ggcttattct ttagggagga tctatctcgt tgctcaagtg catggcgcaa 28740 agcagttatt ggagtgcgga ttttcgttgg catattattc gccacgagtt agcagacgaa 28800 gagaccttca tgaacgtggc caaggccaga gttaagcaga tgttacgccc tgctgcaggc 28860 ctttctatta tccaatagtt ggttgatctt tggaaagagc ctcggctgag gcccatactg 28920 aaagagatcg atggatatcg atatgccatg ttgtttgcta gccagaacca gatcacatcc 28980 gatatgctat tgcagcttga cgcagatatg gaggataagt tgattcatgg gatcgctcag 29040 gagtagctca tccatgcacg ccgacaagaa cagaaattcc gtcgagttaa cgcagccgct 29100 tacgacggat tcgaaggtca tccattcgga atgtattagt ttgcaccagc tccgcgtcac 29160 acctgtcttc atttgaataa gatgttagca attgttttta gctttgtctt gttgtggcag 29220 ggcggcaagt gcttcagaca tcattctgtt ttcaaatttt atgctggaga acagcttctt 29280 aattcctttg gaaataatag actgcgtctt aaaattcaga tgtctggata tagatatgat 29340 tgtaaaataa cctatttaag tgtcatttag aacataagtt ttatgaatgt tcttccattt 29400 tcgtcatcga acgaataaga gtaaatacac cttttttaac attacaaata agttcttata 29460 cgttgtttat acaccgggaa tcatttccat tattttcgcg caaaagtcac ggatattcgt 29520 gaaagcgaca taaactgcga aatttgcggg gagtgtcttg agtttgcctc gaggctagcg 29580 catgcacata gacacacaca tcatctcatt gatgcttggt aataattgtc attagattgt 29640 ttttatgcat agatgcactc gaaatcagcc aattttagac aagtatcaaa cggatgtgac 29700 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgattt 29760 acaattgaat atatcctgcc ccagccagcc aacagctcga tttacaattg aatatatcct 29820 gccggccggc ccacgcgtgt cgaggaattc tgatctggcc cccatttgga cgtgaatgta 29880 gacacgtcga aataaagatt tccgaattag aataatttgt ttattgcttt cgcctataaa 29940 tacgacggat cgtaatttgt cgttttatca aaatgtactt tcattttata ataacgctgc 30000 ggacatctac atttttgaat tgaaaaaaaa ttggtaatta ctctttcttt ttctccatat 30060 tgaccatcat actcattgct gatccatgta gatttcccgg acatgaagcc atttacaatt 30120 gaatatatcc tgccgccgct gccgctttgc acccggtgga gcttgcatgt tggtttctac 30180 gcagaactga gccggttagg cagataattt ccattgagaa ctgagccatg tgcaccttcc 30240 ccccaacacg gtgagcgacg gggcaacgga gtgatccaca tgggactttt aaacatcatc 30300 cgtcggatgg cgttgcgaga gaagcagtcg atccgtgaga tcagccgacg caccgggcag 30360 gcgcgcaaca cgatcgcaaa gtatttgaac gcaggtacaa tcgagccgac gttcacgcgg 30420 aacgaccaag caagcttggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc 30480 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc 30540 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt 30600 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg 30660 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg 30720 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc 30780 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat 30840 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct 30900 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc 30960 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag 31020 gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc 31080 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt 31140 cggaaagggt cgacggatct tttccgctgc ataaccctgc ttcggggtca ttatagcgat 31200 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt 31260 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc 31320 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa 31380 tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc 31440 tgatgaaacc aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga 31500 cgaacgaaga gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct 31560 gctggccgtc ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga 31620 gctggcccgc atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac 31680 cgacgacccg cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat 31740 cgaagagaag caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga 31800 gccatgactt ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc 31860 ccatgcgctc catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc 31920 ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata 31980 aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg 32040 ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga 32100 aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg 32160 caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc 32220 agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg 32280 cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga 32340 agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg 32400 aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc 32460 agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc 32520 gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc 32580 cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct 32640 acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt 32700 acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg 32760 acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc 32820 tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc 32880 tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga 32940 tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga 33000 agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg 33060 agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg 33120 tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg 33180 tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt 33240 cagttccggc tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca 33300 ctgctcgacg cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact 33360 gccgataaac agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg 33420 agcggccgac gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga 33480 gatgttcggg tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg 33540 gttgcgagat gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt 33600 cttcaaacag gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt 33660 ggagcccgaa cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg 33720 tttattgctc gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt 33780 catcctcggc gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg 33840 cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat 33900 ctctgccgct ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg 33960 aactgcgggc gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt 34020 cgcagcgggc ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg 34080 gcaacctccc gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct 34140 gctcgttcca gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt 34200 tctcggcctg gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg 34260 ggggatctcg cgactcgaac ctacagttgt ttccttactg ggctttctca gcccccgagc 34320 gcttagtggg aatttgtacc ccttatcgaa ccgggagcac aggatgacgc ctaacaattc 34380 attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat catgagggaa 34440 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat 34500 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag 34560 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg 34620 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc 34680 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct 34740 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag 34800 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt 34860 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt 34920 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag 34980 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg 35040 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc 35100 atacttgaag ctaggcaggc ttatcttgga caagaagatc gcttggcctc gcgcgcagat 35160 cagttggaag aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa 35220 tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga 35280 gagctgggga agactatgcg cgatctgttg aaggtggttc taagcctcgt cttgcgatgg 35340 catttcgatc cattcccatt ccgcgctcaa gatggcttcc cctcggcagt tcatcagggc 35400 taaatcaatc tagccgactt gtccggtgaa atgggctgca ctccaacaga aacaatcaaa 35460 caaacataca cagcgactta ttcacacgag ctca 35494 <210> 60 <211> 37935 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB109507 <400> 60 gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat ctcagcatgt 60 gcgtagcacg cggccgcctc ccagtatcat tatagtgaaa gttttggctc tctcgccggt 120 ggttttttac ctctatttaa aggggttttc cacctaaaaa ttctggtatc attctcactt 180 tacttgttac tttaatttct cataatcttt ggttgaaatt atcacgcttc cgcacacgat 240 atccctacaa atttattatt tgttaaacat tttcaaaccg cataaaattt tatgaagtcc 300 cgtctatctt taatgtagtc taacattttc atattgaaat atataattta cttaatttta 360 gcgttggtag aaagcataat gatttattct tattcttctt catataaatg tttaatatac 420 aatataaaca aattctttac cttaagaagg atttcccatt ttatatttta aaaatatatt 480 tatcaaatat ttttcaacca cgtaaatctc ataataataa gttgtttcaa aagtaataaa 540 atttaactcc ataatttttt tattcgactg atcttaaagc aacacccagt gacacaacta 600 gccatttttt tctttgaata aaaaaatcca attatcattg tatttttttt atacaatgaa 660 aatttcacca aacaatgatt tgtggtattt ctgaagcaag tcatgttatg caaaattcta 720 taattcccat ttgacactac ggaagtaact gaagatctgc ttttacatgc gagacacatc 780 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 840 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 900 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 960 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 1020 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 1080 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 1140 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 1200 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 1260 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 1320 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 1380 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 1440 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 1500 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 1560 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 1620 ccaaaaatgg cagcgaggct gcaagagcag aaaggaggtg agatggacac gaggattgcg 1680 atcataggca tgagcgccat acttccttgt ggaaccacgg tgagagagtc atgggagacc 1740 ataagggctg gcattgattg cctgtctgat cttcctgagg atcgtgttga tgtgactgcg 1800 tactttgacc cagtcaagac cacaaaggac aaaatctact gcaagagagg aggtttcatt 1860 cctgagtatg actttgatgc gcgtgagttt ggtctcaaca tgttccagat ggaagattct 1920 gatgcgaatc agaccatctc acttctcaag gtcaaagaag cacttcaaga tgctgggatt 1980 gatgcacttg gaaaggagaa gaaaaacata ggatgcgtgc ttggcattgg aggtggacag 2040 aagagttcac atgagttcta cagcagactc aactatgtgg ttgtggagaa agtcttgaga 2100 aagatgggga tgccagagga agatgtcaaa gtggctgtgg agaagtacaa agcgaacttc 2160 ccagaatgga gacttgactc tttccctgga ttccttggga atgtcactgc tggcagatgc 2220 accaacacat tcaacttgga tggcatgaac tgcgttgtgg atgctgcatg tgcaagctcc 2280 ttgatagcag tcaaggttgc gattgatgaa ctgctctatg gtgactgcga catgatggtc 2340 actggtgcaa cctgcacaga caactccatt gggatgtaca tggctttctc aaagacacct 2400 gtgttcagca cggacccaag tgtgagagcg tatgatgaga aaacgaaagg gatgctcatt 2460 ggtgagggat cagccatgtt ggtgctcaaa cgttatgcag atgcagtgag ggatggtgat 2520 gagattcatg ctgtgattcg tggttgtgca agctcaagtg atggcaaagc agctggcatc 2580 tacactccca caatcagtgg tcaagaggaa gccttgagac gtgcatacaa tcgtgcctgc 2640 gttgaccctg ccactgtcac tttggttgag ggtcatggga ctggcactcc agttggagat 2700 aggattgagt tgactgcgtt gaggaacttg tttgacaaag cgtatggtga agggaacaca 2760 gagaaagttg cagttggaag tatcaagtcc agtattggtc atttgaaggc tgttgctggt 2820 cttgctggaa tgatcaaggt catcatggct ctcaaacaca agactttgcc tggaacgatc 2880 aatgtggaca atccacccaa tctctatgac aacactccca tcaatgagtc atccctctac 2940 atcaacacga tgaacagacc ctggttccct ccacctggtg ttccgagacg tgctggaata 3000 tccagctttg gctttggagg tgccaactac catgcagtgt tggaagaggc tgaaccagag 3060 cacacgacag cgtacagact caacaaacgt ccacaaccgg tgttgatgat ggctgcaact 3120 cctgctgcgt tgcagagcct ttgtgaagct cagctgaaag agtttgaagc tgcgatcaag 3180 gagaatgaga cagtcaagaa tactgcctac atcaagtgtg tcaagtttgg agaacagttc 3240 aagttccctg ggagcatacc tgcgaccaat gcgagactgg gattcttggt caaagatgcg 3300 gaggatgcgt gctccacgct tcgtgctata tgtgctcagt ttgccaaaga tgtgaccaaa 3360 gaagcgtgga ggcttccgag agaaggagtt tctttccgtg ccaaaggcat tgccaccaat 3420 ggtgctgttg ctgcactctt ctctggacaa ggtgctcagt acacacacat gttcagtgaa 3480 gttgcgatga actggcctca gttcagacag tccattgctg caatggacgc tgcacagtcc 3540 aaggttgctg gaagtgacaa agactttgaa agagtctctc aagtcctgta cccaaggaaa 3600 ccgtacgaac gtgagccaga acaagatcac aagaaaatct cactcacagc atacagccag 3660 ccgtcaaccc tggcatgtgc tcttggtgcc tttgagattt tcaaggaagc tggattcact 3720 ccagactttg cagctggaca cagcttgggt gagtttgctg cgctctatgc agctggttgt 3780 gttgacagag atgagttgtt tgaacttgtt tgcagaaggg caaggatcat gggaggcaaa 3840 gatgcacctg ccacacccaa aggttgcatg gcagctgtca taggacccaa cgcagagaac 3900 atcaaggttc aagctgccaa cgtctggctt ggaaactcaa actctccgtc tcagactgtc 3960 attactgggt ctgttgaagg gattcaagct gaaagtgcca gattgcagaa ggaagggttc 4020 agagttgtgc ctcttgcttg tgagtctgcc ttccattctc cacagatgga aaatgccagc 4080 tcagctttca aagatgtcat atccaaggtt tccttccgta cacccaaagc agaaacaaag 4140 ctcttcagca atgtttctgg agagacctat ccgacggatg ccagagagat gctcactcaa 4200 cacatgacct catctgtgaa gttcctgacc caagtgagga acatgcacca agctggtgcg 4260 agaatctttg ttgagtttgg accgaaacaa gttctcagca aacttgtttc tgaaaccctc 4320 aaagatgacc cgtctgttgt cacagtgtct gtgaacccag cttctggcac ggactctgac 4380 atacagttga gggatgctgc agttcagctg gttgtggctg gtgtcaatct tcaagggttt 4440 gacaagtggg atgctcctga tgccacgagg atgcaagcca tcaagaaaaa gagaaccaca 4500 ctcagacttt cagctgccac ctatgtcagt gacaagacca agaaagtgag ggatgctgcc 4560 atgaacgacg gaagatgtgt cacatacctc aagggtgctg caccactcat caaggctcct 4620 gaacctgtgg ttgacgaagc tgccaagagg gaggctgaaa ggcttcagaa ggagcttcaa 4680 gacgctcaga ggcagcttga cgacgccaaa cgtgcagctg cagaagccaa ctccaaactt 4740 gctgctgcca aggaggaagc caagactgct gcagcttcag ccaagcctgc agttgacact 4800 gctgttgtgg agaaacatcg tgccatcctc aagtccatgc tggctgagct ggatggttat 4860 ggaagtgttg atgcttcttc ccttcagcaa cagcaacagc aacagactgc tccggcgcct 4920 gttaaagccg ctgcccctgc cgctcccgtt gcaagtgctc ctgctcccgc cgtgtccaac 4980 gagttgcttg agaaagctga gacagttgtg atggaggttc tcgctgccaa gactggatac 5040 gaaacagaca tgattgaggc ggacatggag cttgagactg agttgggaat tgacagcatc 5100 aagagggttg aaattctttc tgaagtccaa gctatgctta acgttgaggc gaaagatgtt 5160 gatgctctct ctaggacgag aacagtgggt gaagttgtca acgctatgaa ggcagaaatc 5220 gctggttctt ccgctccggc gccggcagct gccgcacctg caccggctaa agcggctcca 5280 gctgcagctg caccagctgt ttctaatgag ctgctcgaaa aggcagaaac tgttgtgatg 5340 gaagtccttg ctgccaaaac tggctacgaa actgacatga tagaatcaga catggagctt 5400 gagacggagc ttggaattga ttccatcaag cgtgtggaaa tcctcagtga agtccaagcc 5460 atgctcaatg tggaagctaa ggatgtggat gctctctcaa ggaccagaac tgtcggtgag 5520 gttgtcaatg ccatgaaggc tgagatagct ggaggctccg ctcctgcgcc ggccgctgcc 5580 gctcctggtc ccgctgccgc ggctccagct ccagctgcag ccgctccggc cgtctcaaac 5640 gaactccttg aaaaagctga gaccgtcgtg atggaggtct tggcagctaa aaccggctat 5700 gagactgaca tgattgagtc tgacatggaa ttggagacag aacttgggat agacagcatc 5760 aaacgtgttg aaatcctcag tgaggtccaa gctatgctga acgtggaagc gaaggatgtt 5820 gatgctctgt ccagaacgag gaccgttggt gaggttgtgg atgccatgaa ggctgaaatt 5880 gctggagggt ctgcgcccgc gccagctgca gccgctcctg cacccgcagc cgcggcacct 5940 gctccagctg cacctgcacc tgccgtttct tcagaactct tggagaaggc tgagacggtt 6000 gtgatggagg ttttggctgc aaagactggc tatgagaccg acatgattga atctgatatg 6060 gaattggaga cagagcttgg gatcgattcc atcaaaagag tggagatcct gagtgaagtc 6120 caagccatgc tcaatgttga agctaaggat gttgatgcac tttcaagaac aaggacagtg 6180 ggtgaggttg tggatgccat gaaggcggag attgctggag ggagcgcgcc tgcgccagct 6240 gccgcggcac ctgcaccggc agccgcagct ccagctccag ctgcaccagc accggccgcg 6300 cccgcgccag ctgtttcatc cgaactcctg gaaaaggccg agacggttgt gatggaggtg 6360 ctcgcagcta agactggtta cgagaccgac atgattgaaa gtgacatgga gcttgaaacc 6420 gagttgggaa tagatagcat caaacgtgtt gaaatcttgt ctgaggtcca agctatgttg 6480 aacgtggagg caaaagatgt cgatgcgctt tcaagaacca gaacagtcgg tgaggtcgtg 6540 gacgccatga aggctgaaat tgccggaagc tctgctagtg cgccagctgc agccgcacct 6600 gcacctgctg ccgcggcacc tgctccagct gcagccgctc ctgccgtcag caatgagttg 6660 ctggagaaag ccgaaacagt tgtgatggaa gtccttgctg caaaaactgg ttacgaaacg 6720 gacatgattg agtcagacat ggaattggag accgaacttg gaatagattc catcaagaga 6780 gtggagatcc tctctgaggt gcaagccatg ctcaacgttg aggctaaaga tgttgatgca 6840 ctcagtagga caagaactgt gggggaagtt gtcgatgcga tgaaggctga gattgctgga 6900 ggctctgctc ctgcgccggc agctgccgca cctgcacccg cagccgcggc accagctgtc 6960 agcaacgaat tgcttgagaa ggctgaaaca gttgtcatgg aagtgctggc tgcaaaaacc 7020 ggctatgaga ctgacatgat tgagtctgac atggaacttg agacggaatt gggcattgac 7080 agcatcaagc gtgttgagat cctcagtgaa gtccaagcaa tgctcaatgt ggaggctaag 7140 gatgtggatg ccctctcaag gaccagaaca gttggtgagg tcgttgatgc gatgaaggcg 7200 gagatcgctg gaagttccgc gcctgcgcca gctgcagccg ctcctgcacc cgcagccgcg 7260 gcaccggctc cagctgcagc cgcaccggcc gtctcctcag aactcctgga aaaggctgag 7320 acagttgtga tggaagtcct tgctgcgaaa actggctatg agaccgacat gattgaatct 7380 gacatggaac tggagaccga gcttggaatt gactccatca aacgtgttga aatcctctct 7440 gaggttcaag cgatgttgaa tgtggaggcc aaagatgttg atgctctttc cagaacaagg 7500 acggtgggag aggtggttga tgccatgaag gctgagatag ctgggggttc tgctcctgcg 7560 ccagctgcag ccgctcctgc tcccgctgcc gcggcaccag ctgtcagcaa cgaactcctt 7620 gaaaaagctg aaactgttgt gatggaggtt ttggctgcaa agactggata cgagactgac 7680 atgatagagt cagacatgga gttggaaaca gagcttggga ttgatagcat caagcgtgtg 7740 gaaatccttt ctgaggttca agccatgctg aacgttgagg ccaaagatgt cgatgctttg 7800 tcaaggacca gaacggttgg agaagtggtc gatgccatga aggctgagat agctggtgga 7860 tcagcccctg ctccggctgc agccgctcct gcaagtgccg gtgctgcacc agctgtgaag 7920 attgattcag ttcatggtgc tgactgtgac gatttgtcac tgatgcacgc aaaggtggtt 7980 gacatcagaa ggccagatga actgattctg gaaagaccgg agaaccgtcc agttcttgtc 8040 gttgacgatg gctcagagtt gacacttgct cttgtgaggg tgttgggagc ctgtgcagtt 8100 gtcttgacct ttgagggatt gcagttggct cagagagctg gtgcagctgc cattcgtcat 8160 gttctggcta aggacctttc agcagaatca gctgagaagg ccatcaaaga ggctgagcaa 8220 agatttggag cgttgggagg cttcataagc cagcaagcag aaaggtttga accagctgag 8280 atactgggtt tcacactcat gtgtgcgaag tttgccaaag cctctctctg cactgctgtg 8340 gctggaggtc gtcctgcctt catcggtgtg gctaggcttg acggcagact tggattcacc 8400 tctcaaggca cttctgacgc actcaagaga gcacagcgtg gagcgatctt tggactctgc 8460 aagacaatag ggttggagtg gtcagaatct gatgtgttca gccgtggtgt ggacattgct 8520 caagggatgc acccagaaga tgctgcagtt gccatagtta gggagatggc gtgtgctgac 8580 atcagaatca gagaggttgg gattggagca aaccagcaaa gatgcacaat cagagctgcc 8640 aagttggaga ctgggaaccc tcagagacag atagcgaaag acgatgtgct tctggtttct 8700 ggaggtgctc gtgggatcac acctctttgc atccgtgaga tcacgcgtca gattgctgga 8760 ggcaagtaca ttctgttggg tcgtagcaaa gtttctgcgt ctgaaccagc atggtgtgct 8820 ggcatcactg atgagaaggc tgtgcagaaa gctgccaccc aagagttgaa gagggccttc 8880 agtgctggtg aaggacccaa acccactcca agggctgtca cgaaacttgt gggatctgtt 8940 cttggtgctc gtgaagtgag aagctccatt gctgccattg aagctctggg tggcaaagcg 9000 atctacagct cttgtgatgt gaactctgct gcagatgttg ccaaagcagt tagggatgca 9060 gagtcccagc ttggtgccag agttagtgga atcgttcacg cttctggtgt gctccgtgat 9120 cgtctcatag agaagaaact ccctgacgag tttgatgctg tctttgggac caaagtcact 9180 gggttggaga atcttctggc tgcagttgat cgtgccaacc tcaaacacat ggttctcttc 9240 agctcccttg ctggtttcca cggcaatgtt ggtcagtctg actatgcgat ggcaaacgaa 9300 gctctcaaca agatgggttt ggaacttgcg aaggatgtca gtgtcaagtc catctgcttt 9360 ggtccctggg atggtgggat ggtgacacct caactcaaga aacagtttca agaaatggga 9420 gttcagatca ttcctcgtga aggaggtgct gatacagttg cgaggattgt gttgggatca 9480 agtcctgctg agatccttgt tggaaactgg aggacaccat ccaagaaagt tggaagtgac 9540 acgatcaccc tgcatagaaa gatcagtgcc aagtccaatc ccttcttgga ggatcatgtc 9600 atacaaggca gaagggtgtt gccaatgacc cttgctattg ggagccttgc tgagacctgc 9660 cttggactct tccctggcta ctctctctgg gccatagatg acgcacagct cttcaaaggt 9720 gtcactgttg atggagacgt caactgtgaa gtgaccctca caccgtccac tgctccgagt 9780 gggagggtga atgtccaagc gacgctcaag acgttctcaa gtggaaaact tgttccagcc 9840 tacagagctg tcatagtgtt gtccaaccaa ggtgctccac ctgccaatgc aaccatgcag 9900 cctccgagcc ttgatgcaga tcctgctttg caagggtcag tctatgatgg caagactctc 9960 tttcatggtc ctgcgttcag aggcattgat gacgttctca gctgcaccaa gagccagctt 10020 gttgccaagt gttctgctgt tcctggatca gatgcagcca gaggagagtt tgcaactgac 10080 actgatgcac atgatccctt tgtcaatgat cttgcctttc aagcaatgtt ggtctgggtg 10140 aggagaacgc tgggacaagc tgcactcccg aactccattc agaggatagt ccaacacaga 10200 ccggtcccac aagacaaacc cttctacatc accctcagat ccaatcagag tggtggacat 10260 agccagcaca aacatgcact tcagttccac aacgaacaag gtgatctctt cattgatgtt 10320 caagcctctg tcattgcgac agactcactt gcgttctgag tagttagctt aatcacttag 10380 gtcaccagta tgaactaaaa tgcatgtagg tgtaagagct catggagagc atggaatatt 10440 gtatccgacc atgtaacagt ataataactg agctccatct cacttcttct atgaataaac 10500 aaaggatgtt atgatatatt aacactctat ctatgcacct tattgttcta tgataaattt 10560 cctcttatta ttataaatca tctgaatcgt gacggcttat ggaatgcttc aaatagtaca 10620 aaaacaaatg tgtactataa gactttctaa acaattctaa ctttagcatt gtgaacgaga 10680 cataagtgtt aagaagacat aacaattata atggaagaag tttgtctcca tttatatatt 10740 atatattacc cacttatgta ttatattagg atgttaagga gacataacaa ttataaagag 10800 agaagtttgt atccatttat atattatata ctacccattt atatattata cttatccact 10860 tatttaatgt ctttataagg tttgatccat gatatttcta atattttagt tgatatgtat 10920 atgaaaaggt actatttgaa ctctcttact ctgtataaag gttggatcat ccttaaagtg 10980 ggtctattta attttattgc ttcttacaga taaaaaaaaa attatgagtt ggtttgataa 11040 aatattgaag gatttaaaat aataataaat aataaataac atataatata tgtatataaa 11100 tttattataa tataacattt atctataaaa aagtaaatat tgtcataaat ctatacaatc 11160 gtttagcctt gctggaacga atctcaatta tttaaacgag agtaaacata tttgactttt 11220 tggttattta acaaattatt atttaacact atatgaaatt tttttttttt atcagcaaag 11280 aataaaatta aattaagaag gacaatggtg tcccaatcct tatacaacca acttccacaa 11340 gaaagtcaag tcagagacaa caaaaaaaca agcaaaggaa attttttaat ttgagttgtc 11400 ttgtttgctg cataatttat gcagtaaaac actacacata acccttttag cagtagagca 11460 atggttgacc gtgtgcttag cttcttttat tttatttttt tatcagcaaa gaataaataa 11520 aataaaatga gacacttcag ggatgtttca acccttatac aaaaccccaa aaacaagttt 11580 cctagcaccc taccaacgaa ttcgcggccg cttaattaag atgagtgata ctcaggactc 11640 aggactcact ctgctgatca ctagtgctag cctcgaggtc gacacaactt tgtatacaaa 11700 agttgtctag acctaggtta attaagcggc cgcagcttat ttctcttttc gatactctaa 11760 ttgagccgtg cgctctatct agaccaatta gaattgatgg agctctaaag gttgctggct 11820 gttttcttgt tcatatgatt aacttctaaa cttgtgtata aatattctct gaaagtgctt 11880 cttttggcat atgtaggttg ggcaaaaacg aggaagattg cttctcaatt tggaagatga 11940 tgaacagccg aagaagaaaa taagaatagg cagtcctgct actcaatgga tctcagtcta 12000 taacggtcgt cgtcccatga aacagaggta acacattttt tgcatataca ctttgatagt 12060 tcctcactaa ctgtgtaatc ttttggtaga tatcactaca atgttggaga gacaaggctg 12120 cgccagcata tacagaaggg aaatgaagat ggccttttga ttagctgtgt agcatcagca 12180 gctaatctct gggctctcat catggatgct ggaactggat tcacttctca agtttatgag 12240 ttgtcaccgg tcttcctaca caaggtaata atcagttgaa gcaattaaga atcaatctga 12300 tttgtagtaa actaagaaga acttacctta tgatttcccc gcaggactgg attatggaac 12360 aatgggaaaa gaactactat ataagctcca tagcgggttc agataacggg agctctttag 12420 ttgttatgtc aaaaggttag tgtttagtga ataataaact tattatcaaa aagtcttcat 12480 tgacttattt atatacttgt tgtgaattgg taggaactac ttattctcag cagtcataca 12540 aagtgagtga ctcatttcca ttcaagtgga taaataagaa atggaaagaa gattttcatg 12600 taacctccat gacaactgct ggtaatcgtt ggggtgtggt aatgtcgagg aactctggct 12660 tctctgatca ggtaggtttt tgtctcttat tgtctggtgt ttttattttc ccctgatagt 12720 ctaatatgat aaactctgcg ttgtgaaagg tggtggagct tgactttttg tacccaagcg 12780 atgggataca taggaggtgg gagaatgggt atagaataac atcaatggca gcaactgcgg 12840 atcaagcagc tttcatatta agcataccaa agcgtaagat ggtggatgaa actcaagaga 12900 ctctccgcac caccgccttt ccaagtactc atgtcaaggt tggtttcttt agctttgaac 12960 acagatttgg atctttttgt tttgtttcca tatacatagg acctgagagc ttttggttga 13020 attttttttt tttcaggaca aatgggcgaa gaatctgtac attgcatcaa tatgctatgg 13080 caggacagtg tgctgatgat acacacttaa gcatcatgtg ttgtgttaga aagccgaaga 13140 caattggagc gagcctcagg gtcgtcataa taccaatcaa agacgtaaaa ccagacgcag 13200 tctctttggt tgaatgtgat gaaagggatg tgtcttggta tgtatgtacg agtaacaaaa 13260 gagaagatgc aattgagtag tagaaagatt tgagagcttt ttaaagccct tcaagtgtgt 13320 gcttttatct tattgatatc atccatttgc gttgtttaat gcgtctttag atatgtttct 13380 gtttctttct cagtgtctga atatctgata agtgcaatgt gagaaagcca caccaaacca 13440 aaatattcaa atcttatatt tttaataatg tcgaatcact cggagttgcc accttctgtg 13500 ccaattgtgc tgaatctatc acactaaaaa aaacatttct tcaaggtaat gacttgtgga 13560 ctatgttctg aattctcatt aagtttttat tttttgaagt ttaagttttt accttctttt 13620 ttgaaaaata tcgttcataa gatgtcacgc caggacatga gctacacatc acatattagc 13680 atgcagatgc ggacgatttg tcactcactt caaacaccta aaagagcttc tctctcacag 13740 cacacacaca tatgcatgca atatttacac gtgatcgcca tgcaaatctc cattctcacc 13800 tataaattag aggctcggct tcacttttta ctcaaaccaa aactcatcac tacaagatcc 13860 acaaccaaaa atggctgcaa ggaacgtgag tgcggctcat gagatgcatg acgagaagag 13920 gatagctgtt gtgggcatgg ctgttcagta tgctggatgc aaaacgaaag acgagttctg 13980 ggaagttctc atgaatggaa aagttgagag caaagtgatc agcgacaaac gtcttggttc 14040 aaattaccgt gctgaacact acaaagctga aaggagcaag tatgctgaca ccttctgcaa 14100 tgagacatat ggaactcttg atgagaacga aatagacaat gagcatgaac tccttttgaa 14160 ccttgccaaa caagccttgg ctgaaacatc tgtcaaagat tcaacaagat gtggcatagt 14220 ttctggatgc ctcagcttcc cgatggacaa cctccaaggt gaactcttga atgtgtacca 14280 gaaccatgtt gagaagaaac ttggtgccag agttttcaaa gatgcctctc actggtcaga 14340 aagagaacag tccaacaaac cagaggctgg tgacagaaga atcttcatgg accctgcttc 14400 ctttgttgct gaggaactca atcttggcgc tctccactac tctgttgatg ctgcctgtgc 14460 cactgcgctc tatgtgctca gactggcgca agatcacttg gtctctggtg cagctgatgt 14520 gatgctttgt ggtgcaacat gcctgccgga gccgtttttc atactctctg ggttctcaac 14580 cttccaagcc atgcctgttg gaacgggtca gaatgtgtcc atgccgctgc acaaagattc 14640 tcaaggattg acccctggtg aaggaggttc catcatggtc ctcaaacgtt tggatgacgc 14700 aatcagagat ggagatcaca tctatggaac acttttggga gccaatgtca gcaacagtgg 14760 cactggtctg ccacttaaac cccttttgcc gtcagagaaa aagtgcctca tggacaccta 14820 cacacgtatc aatgtgcatc cccacaagat tcagtatgtt gaatgccatg caactgggac 14880 tccacaaggt gatcgtgtgg aaattgatgc tgtgaaggct tgctttgaag gcaaagtccc 14940 acgttttgga accacaaaag ggaactttgg acacacattg gtggcagctg ggtttgctgg 15000 catgtgcaaa gtgcttctct ccatgaagca cgggatcatt ccacccactc cgggaataga 15060 tgacgaaacg aagatggacc ctctggttgt ctctggtgag gccattccct ggccagaaac 15120 gaatggagaa ccgaagcgtg ctggtctctc agcgtttgga tttggtggga ccaatgctca 15180 tgctgtcttt gaggaacatg acccaagcaa tgctgcatgc actggccatg actccatttc 15240 tgctctctct gcacgttgtg gtggtgagtc aaacatgaga atagcaatca ctgggatgga 15300 tgccactttt ggtgctttga aaggattgga tgcttttgaa agggccatct acactggtgc 15360 tcatggtgct ataccactcc cagaaaagag atggagattc ctggggaaag acaaggactt 15420 ccttgatctt tgtggagtca aagcaacacc gcatggctgt tacattgaag atgttgaagt 15480 tgatttccag aggctgagga ctccaatgac tcctgaagat atgctcttgc ctcaacagct 15540 tctggctgtc accacaattg atagggccat attggactct ggcatgaaga aaggtggaaa 15600 tgtggctgtc tttgtggggt tggggaccga tcttgaactc taccgtcacc gtgcaagggt 15660 tgctctcaag gagagagtga ggcctgaagc cagcaagaaa ctcaatgaca tgatgcagta 15720 catcaatgat tgtgggacaa gcacttctta caccagctac ataggcaact tggttgcaac 15780 aagggtcagt tcacaatggg gcttcacggg accttccttc acaatcacag aaggcaacaa 15840 ttctgtgtac agatgtgctg aacttggcaa atacctcttg gaaacgggag aggttgatgg 15900 ggttgtggtt gcgggtgttg atctctgtgg gagtgctgag aacctttacg tcaagtcaag 15960 gcgtttcaag gtgtctacat ctgacacccc gagagccagc tttgatgcag ctgctgatgg 16020 ttactttgtt ggagagggct gtggtgcatt tgtgctcaag cgtgagacaa gctgcaccaa 16080 ggatgacaga atctatgctt gcatggatgc catagttcct ggaaatgttc catctgcatg 16140 tttgagagag gcattggatc aagccagagt caagcctgga gacatcgaga tgttggagct 16200 ttctgctgat tcagcgaggc atttgaagga cccgtctgtt ctcccaaagg aactcactgc 16260 tgaggaagag attggcggac ttcagacgat tcttcgtgac gatgacaagc tcccaaggaa 16320 tgttgcaact ggaagtgtca aggccactgt tggtgacact ggttatgctt ctggtgctgc 16380 cagtttgatt aaggctgcgc tttgcatcta caaccgttac ctgcccagca atggagatga 16440 ctgggatgaa cctgcacctg aagcaccctg ggacagcaca ctctttgcgt gccagacctc 16500 aagagcttgg ttgaagaacc ctggtgagag acgttatgcg gctgtttccg gagtcagtga 16560 aaccagaagt tgctacagtg tgctgctttc tgaagcagag ggacactacg agagagagaa 16620 cagaatcagt ttggatgagg aagcacccaa gctcattgtg ttgagagcag actcccatga 16680 agagattctt ggaagattgg acaagataag ggaaaggttc ttgcagccca cgggtgctgc 16740 acccagagag tctgaattga aggcgcaagc ccgtaggatc tttcttgaac tccttggtga 16800 gacccttgcc caagatgcag cttcatctgg aagtcagaag ccccttgctc tcagcttggt 16860 ctccactcca tccaagctcc agagagaggt ggaacttgcg gcaaaaggca ttccgagatg 16920 tctcaagatg agacgtgatt ggagttcccc tgctggaagc agatatgcgc ctgagccttt 16980 ggccagtgac agagttgcct tcatgtatgg agaaggaagg agcccttact atggcatcac 17040 acaagacatt cataggattt ggcctgaact tcatgaggtc atcaatgaga aaacaaaccg 17100 tctctgggct gaaggtgaca gatgggtcat gccaagggcc agcttcaaga gtgagcttga 17160 gtcacaacag caagagtttg acagaaacat gattgaaatg ttccgtcttg gaatcctcac 17220 ctcaattgcc ttcacaaacc tggcgagaga tgttctcaac atcacgccca aagcagcctt 17280 tggactcagt ttgggagaga tttccatgat ctttgctttc tccaagaaaa acggtttgat 17340 aagcgatcag ttgacaaagg atttgagaga gtcagatgtc tggaacaaag ctctggctgt 17400 tgagttcaat gcgctcagag aagcctgggg cattccacag tctgtcccaa aagatgagtt 17460 ctggcaaggt tacatagtga ggggaacgaa acaagacata gaagcagcca tagcaccaga 17520 ctcaaagtat gtgaggttga ccatcatcaa tgatgccaac actgctctca tcagtggaaa 17580 gcctgatgca tgcaaagctg cgattgctcg tcttggtggc aacatccctg cgcttccagt 17640 cactcaaggc atgtgtgggc attgcccaga ggtgggacct tacaccaagg acatcgcaaa 17700 gatccacgcc aaccttgagt tccctgtggt tgatggtctt gatctctgga ctaccataaa 17760 ccagaagagg cttgtgccaa gagccacggg tgccaaagat gaatgggctc cttcatcctt 17820 tggggagtat gctggacagc tctatgagaa acaagccaac ttccctcaga tagttgaaac 17880 catctacaag cagaactatg atgtgtttgt tgaagttggg ccaaacaatc acagaagcac 17940 agcagtgaga accacgcttg gaccacagag aaaccacctt gctggtgcca tagacaagca 18000 gaacgaggat gcttggacga caatagtcaa gctcgtggct tcattgaagg ctcaccttgt 18060 tcctggagtg accatcagtc cgctctacca ctccaagctc gttgcagagg ctgaggcttg 18120 ttatgctgcg ctctgtaaag gtgagaaacc caagaaaaac aagtttgtga gaaagattca 18180 gttgaatggc agattcaaca gcaaggcaga ccccatctcc tcagcagacc ttgcatcctt 18240 cccacctgcg gaccctgcga ttgaagctgc catctcttca aggatcatga agcctgtggc 18300 tccgaaattc tatgcgcgtc tcaacattga tgagcaagat gagaccagag atccgatctt 18360 gaacaaggac aatgcgcctt ccagctccag ctcttccagc agttcctctt ccagcagttc 18420 atctccgtct cctgctcctt cagctccagt tcagaagaaa gctgccccag ctgcggagac 18480 aaaagctgtt gccagtgctg atgcacttcg tagtgccctg cttgatcttg actccatgct 18540 tgcgctcagc tctgcaagtg cttctggcaa ccttgttgag actgcgccat ctgatgccag 18600 tgtcattgtc cctccgtgca acattgctga tcttggcagc agagctttca tgaaaactta 18660 tggtgtttct gcgcctctgt acaccggtgc aatggccaag ggaattgcct ctgcggactt 18720 ggtgatcgca gctggaaggc aaggaatcct tgcgtcattt ggtgctggag gccttccaat 18780 gcaagttgtg cgtgagtcca ttgaaaagat tcaagctgcc ctgcccaatg gaccatatgc 18840 tgtcaacctc atccattctc cttttgacag caaccttgag aagggaaatg ttgatctctt 18900 cttggaaaag ggtgtcactt ttgttgaggc ttcagccttc atgacgttga caccgcaagt 18960 tgtcagatac agagcggctg ggttgacgag gaatgcagat gggtcagtca acataaggaa 19020 cagaatcatt gggaaagtct ccagaactga gctggctgaa atgttcatgc gtcctgcacc 19080 tgagcatctt ttgcagaagc tcattgcttc tggggagatc aatcaagaac aagctgagtt 19140 ggccagacgt gttcctgtgg ctgatgacat tgcggttgaa gctgattctg gtggacacac 19200 tgacaaccgt ccaatccatg tcattctgcc tctgattatc aacctgaggg ataggcttca 19260 cagagagtgt ggatacccag caaacctgag ggttcgtgtt ggtgctgggg gtggcattgg 19320 gtgtccccaa gctgcgctgg caactttcaa catgggtgcc tctttcattg tcactggaac 19380 agtgaaccaa gttgccaaac aatctgggac gtgtgacaat gtgaggaaac agcttgcgaa 19440 agcaacttac tcagatgttt gcatggcacc ggcagctgac atgtttgagg aaggtgtgaa 19500 acttcaagtt ttgaagaaag gaacaatgtt cccttcaaga gccaacaaac tctatgagtt 19560 gttctgcaag tatgattctt ttgagtccat gcctccagca gagcttgcga gggttgagaa 19620 aagaatcttc agtcgtgcat tggaagaggt ctgggatgag accaagaact tctacatcaa 19680 ccgtctccac aacccagaga agatacagag ggctgagagg gaccccaaac tgaagatgtc 19740 actgtgtttc agatggtatc tgtccctggc ttcaaggtgg gccaacactg gagcttctga 19800 cagagtcatg gactaccaag tttggtgtgg tcctgccatt ggttcattca atgatttcat 19860 caaaggaact taccttgatc cggcagtggc aaatgagtac ccgtgtgttg tgcagatcaa 19920 caaacagatt cttagaggag cgtgcttctt gaggcgtctt gaaatactga gaaatgcaag 19980 gctttcagat ggtgcagctg cacttgtggc gagcattgat gacacatatg tccctgctga 20040 gaagctgtga gtagttagct taatcactta ggtcaccatt ccaaacgaaa tatcctcgag 20100 agtgtgtata ccacggtgat atgagtgtgg ttgttgatgt atgttaacac tacatagtca 20160 tggtgtgtgt tccataaata atgtactaat gtaataagaa ctactccgta gacggtaata 20220 aaagagaagt tttttttttt actcttgcta ctttcctata aagtgatgat taacaacaga 20280 tacaccaaaa agaaaacaat taatctatat tcacaatgaa gcagtactag tctattgaac 20340 atgtcagatt ttctttttct agatgtctaa tcaagccttc aaggctagtg ataaaagatc 20400 atccaatggg atccaacaaa gactcaaatc tggttttgat cagatacttc gaaactattt 20460 ttgtatgcac taaattatgc aagtgttctt ttatttggtg aagactcttt agaagcaaag 20520 aacgataagc agtaataaaa aaaataaagt tcagttttaa gatttgttat tgacttagcg 20580 gccgcttaat taaactagtg ctagcctcga ggtcgaccaa cttttctata caaagttgtc 20640 tagacctagg actaggcact gagtgactca ctgacctgac tgagtgactg gcggccgcag 20700 cttatagtaa agattttttt gttaataaaa tgaaagttaa tatttatgga tttatttgaa 20760 catatttaca agtttttgtt aataaattta tttacagaag tgttaaattt gaaaatgtgt 20820 taaatgagag ttaatattta tggatataat attactttat ttaaaaataa aatgaattta 20880 tattaaaata tgatttggta aaagatatta taaagaggaa cttggatgag aaagacgacg 20940 atgggcaaaa gttgcatggc acaccgcttt tgttcagtaa catctgcact gtggcgcctt 21000 tttcacactc accatgcatg ctgccacgtc agctttcaca ctatgacacc actggccata 21060 cacgttgcca cctcagcttt ctcctcttct cactatgaca cgactggcca tgcatgcatg 21120 catgctgcca cctcagctcc cgcctcttca cccgtgtctt tctctcatgt cgcagtgctg 21180 cgtgccaacc tcttctctct ataaatagag agaagagaat gatggttaaa ctcaaaccaa 21240 aacaagaaaa catacacaaa tagcaaaacg gatccacaac caaaaatggc gcttcgtgtc 21300 aagaccaaca agaagccatg ttgggagatg accaaggagg aactgacctc tggcaaaact 21360 gaggtgttca actatgagga actcctggag tttgcagagg gtgacatagc taaggttttt 21420 ggtccggagt ttgctgtcat agacaagtac cccagaaggg tgaggctgcc tgctcgtgaa 21480 tacctgcttg tgacaagggt cacactcatg gatgccgagg tcaacaacta cagagttggt 21540 gccagaatgg tcactgagta tgatcttcca gtgaatggcg aattgtctga gggaggtgat 21600 tgtccctggg cagttctggt tgagagtgga cagtgcgatt tgatgctgat atcatacatg 21660 ggaattgatt tccagaacca aggtgacaga gtttaccgtc tgctcaacac cacgttgaca 21720 ttctatgggg ttgcccatga gggagagacc cttgagtatg acatcagagt gaccggtttt 21780 gccaaacgtt tggatggtgg catctccatg ttcttctttg agtatgactg ctatgtcaat 21840 gggaggcttc tcattgagat gagagatggc tgtgctggct tcttcacaaa tgaggagctt 21900 gatgcgggaa agggtgtggt gttcacaaga ggggatttgg ctgccagagc taagattcca 21960 aagcaagatg tttctcccta tgctgtcgct ccctgccttc acaaaacaaa actcaacgaa 22020 aaggagatgc agaccttggt tgacaaagat tgggcttccg tttttggctc aaagaatggc 22080 atgccggaga tcaactacaa actctgcgca cgtaagatgc tcatgattga tagagtcacc 22140 agcattgacc ataagggagg tgtgtacggt ttgggtcagt tggttggtga gaaaatcttg 22200 gagagggacc attggtactt cccatgccat tttgttaagg atcaagtcat ggctggatct 22260 cttgtcagcg acggttgttc acaaatgctc aagatgtaca tgatctggct cggtttgcac 22320 cttaccaccg gaccatttga cttcagaccc gtcaacggac acccgaacaa agttaggtgt 22380 aggggtcaga tatctcctca taagggaaag ttggtctacg tcatggagat caaagagatg 22440 ggattcgacg aggataacga tccgtacgcc attgctgacg tcaacatcat tgacgttgat 22500 ttcgaaaagg gtcaagattt cagccttgat agaatcagcg actacggaaa gggggatctt 22560 aacaaaaaga tcgtcgtgga cttcaagggc attgctctca agatgcagaa gagatccacg 22620 aacaagaacc cctccaaagt tcagcccgtt tttgctaacg gtgcagccac tgtgggaccg 22680 gaagcgtcca aggccagctc tggtgccagt gctagtgctt ctgctgcccc tgcgaagcct 22740 gctttctcag cagatgttct tgcgccaaag ccagttgcac ttcctgaaca tatcctcaag 22800 ggtgacgctc tcgctccgaa agaaatgtcc tggcatccca tgtcaaaact tcctggcaac 22860 ccgacgccgt tcttctcccc tagttcttac cctccgaggg caatttgctt catccctttc 22920 cctggcaacc cacttgacaa caattgcaaa gctggagaaa tgcctctgaa ctggtacaac 22980 atgtcagagt tcatgtgtgg aaaagttagc aactgcttgg gaccagagtt cgcaagattt 23040 gataagtcaa atacgagcag aagccctgct tttgatttgg ctcttgtgac tagagttgtt 23100 gaagtcacaa acatggaaca tggcaagttc ctgaatgttg attgcaatcc cagcaaaggc 23160 acaatggtgg gggagtttga ctgtcctcaa gatgcgtggt tctttgatgg ttcttgcaat 23220 gatgggcaca tgccttattc catcatcatg gaaatcggac ttcagacatc cggtgttctc 23280 accagtgtgt tgaaagcacc actgacgatg gacaaagatg acattctctt taggaacctt 23340 gatgcaagtg ctgaaatggt gaggccagat gtggatgtga gggggaaaac cataaggaat 23400 gtgaccaagt gcactggata tgcaatgttg ggaaagatgg ggattcacag attcacgttt 23460 gagttgtctg ttgatggcgt ggttttctac aaaggaagca cttcctttgg atggttcact 23520 ccagaggtgt ttgctcagca agctggactt gacaatggga aaaagacgga gccttggtgc 23580 aaaactaaca acacgagtgt gagaagagtt gaaatcgcat cagccaaagg caaagaacag 23640 ctgactgaga aacttccaga tgcaactaat gctcaagttc tcagaaggtc agaacagtgt 23700 gagtatttgg attacctcaa cattgctcct gattctgggc tgcatgggaa aggatatgcg 23760 catggacaca aagatgttaa cccacaagac tggttcttct cttgtcactt ttggtttgat 23820 cctgtgatgc ctggatcttt gggaattgag tcaatgttcc agctcattga ggcatttgcg 23880 gtggaccaga acataccggg agaatacaat gtgagcaatc cgacctttgc ccatgcccct 23940 ggaaaaacgg cgtggaagta ccgtggtcaa ttgacaccaa agaaccgtgc gatggactgt 24000 gaggtgcaca tagtttcaat cacagcctct cctgaaaatg gaggctacgt tgacatagtg 24060 gcagatggag cactttgggt tgatggtctc cgtgtgtatg aagccaaaga actcagagtt 24120 cgtgttgtca gtgcaaaacc tcaagccatt ccggatgttc aacaacagcc acccagcgca 24180 aaagcggacc ctgggaaaac tggagttgca ctcagtccga ctcagttgag agatgtcctg 24240 cttgaagtgg acaatccatt gtatcttggt gttgaaaact ccaatttggt gcagtttgaa 24300 tccaaacctg caacttcttc aaggattgtc agtatcaaac catgctccat aagtgatctt 24360 ggtgacaagt ccttcatgga aacgtacaat gtgtcagcac ctctgtacac tggagcaatg 24420 gccaagggaa ttgcgtctgc ggaccttgtc attgctgctg ggaagaggaa gatattgggt 24480 tcttttggag cgggtgggct tccaatgcac catgtgaggg cagctttgga gaagatacaa 24540 gcggctctgc ctcaaggacc atatgctgtg aacctcattc attcaccttt tgacagcaac 24600 ttggaaaagg ggaatgttga tctcttcctc gaaaagggcg ttactgtggt ggaggcttct 24660 gcattcatga ccctcactcc ccaagttgtt cgttaccgtg cagccggact ctcaaggaac 24720 gctgatggga gtgtcaacat cagaaaccgt atcattggaa aagtgtccag aacagaactc 24780 gccgaaatgt tcatcagacc ggctccggaa cacctccttg agaaactcat agcttctggt 24840 gagataacac aagaacaagc cgaacttgcc cgtcgtgttc ctgtcgcaga cgacatcgct 24900 gttgaagctg attctggtgg tcacactgac aaccgtccca tccacgtcat cttgcctttg 24960 atcatcaacc ttaggaacag actgcacaga gagtgtggtt atcctgcgca ccttagagtc 25020 agagttggag ctggaggcgg tgttggctgc ccacaagctg ctgcggctgc cctcacaatg 25080 ggagctgcct tcatagtgac tgggactgtc aatcaagttg ctaaacagtc cgggacatgt 25140 gacaacgtga gaaaacaact cagtcaagcc acctacagtg acatttgcat ggctccagca 25200 gctgatatgt ttgaggaagg tgtgaaactc caagttctca agaagggaac aatgttccca 25260 tcaagagcta acaaattgta cgaactcttt tgcaagtacg actctttcga ttcaatgcct 25320 cctgctgaac ttgaaaggat cgagaagagg atcttcaaga gggcacttca agaggtctgg 25380 gaagagacca aggactttta catcaatggt ttgaagaatc cagagaagat acagagagcg 25440 gaacatgacc ccaaactcaa gatgtctttg tgtttcagat ggtatcttgg tcttgcatca 25500 cgttgggcca acatgggtgc tcctgatcgt gtcatggact atcaagtctg gtgtggtcca 25560 gccattggag ccttcaatga cttcatcaaa ggaacatatc ttgatcctgc tgtctctaat 25620 gagtacccat gtgttgtcca gatcaacctt cagattttgc gtggtgcatg ttacctgagg 25680 cgtcttaatg ctctgagaaa tgatccgagg attgatcttg agactgaaga tgctgccttt 25740 gtctatgagc caacgaatgc gctttgagta gttagcttaa tcacttaggt cacccagtcc 25800 aagcccggcc tagcacgatg atcatgagtg ttgctattga tgtatgttaa tactatacac 25860 agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg tttgaggcta atgtaattag 25920 cactacaccg taataaatga gaggtttact ttatgtttta ataatgttct tacccttgct 25980 gctctctaaa ctgtaatggc gtacggttag ctaaactaaa gtaaaaatcg attcgcttga 26040 gtaaaattag tgaccgggaa agatcatcga ctactactgc tcagagatgt aatcggtaat 26100 taagacttca agctcaataa ttaattagga gtaaatctgg cttcgaattt ttaaattgtc 26160 aacttccaaa gaaggattgg caagactcaa tctcaattgc ccaacaaaca tatggctctt 26220 tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta attcctctgt cgatctcatg 26280 taattaccaa actcatctac ttattacttt ctttatatgc acttactgat gcggccgctt 26340 aattaatcac gagtggatga ctgtcacgac tgactcatga ctgactacta gtgctagcct 26400 cgaggtcgac acaactttgt ataataaagt tgtctagacc taggagctgt gctgggtaac 26460 tcacatgagt cactggtcac atcagcggcc gcagatctga ttggtaagat atgggtactg 26520 tttggtttat atgttttgac tattcagtca ctatggcccc cataaatttt aattcggctg 26580 gtatgtctcg gttaagaccg gtttgacatg gttcatttca gttcaattat gttaatctgg 26640 cacgtgatat gtttaccttc acacgaacat tagtaatgat gggctaattt aagacttaac 26700 aggcctagag aggcccatct tattacgtaa cggcgtcgtt tagagtgcac caagcttata 26760 aatgaaaacg agctacctcg ggacatcacg ctctttgtac actccgccat ctctctctct 26820 cgttggagca gatctctctc gtgaatatcg acaatgcttc agcacacttg gcttccgaag 26880 cctcccaatc tgaccctctt gtcagatgag gttcatctct ggaggattcc tcttgaccag 26940 cctgagtcac aacttcaaga ccttgctgcc accctgagca gtgatgaatt ggcgagggca 27000 aacagattct actttccaga acacagaagg cgtttcactg ctgggagagg catcctcaga 27060 tccatcttgg gtggatactt gggagtggaa ccgggtcaag tcaagtttga ttatgagtcc 27120 cgtgggaaac cgatccttgg tgacagattt gctgagagtg gactcctgtt caacttgtct 27180 cacagccaga accttgcctt gtgtgctgtc aactacacgc gtcaaatagg cattgatctt 27240 gaatatctgc gtccaacatc tgacttggag tctcttgcaa agaggttctt tctcccaaga 27300 gaatatgaac tcttgaggtc actccctgat gagcagaaac agaagatttt ctttcgttac 27360 tggacttgca aagaggctta tctcaaagca acgggagatg gaatagccaa acttgaagag 27420 atcgagatag cactcacccc aacagaacct gccaagctcc aaacagctcc tgcgtggtct 27480 ctgttggagc ttgtgccaga tgacaattgt gttgcagctg tggctgttgc gggttttggt 27540 tggcagccca agttctggca ttactgagta gttagcttaa tcacttaggt caccctatca 27600 aaatctattt agaaatacac aatattttgt tgcaggcttg ctggagaatc gatctgctat 27660 cataaaaatt acaaaaaaat tttatttgcc tcaattattt taggattggt attaaggacg 27720 cttaaattat ttgtcgggtc actacgcatc attgtgattg agaagatcag cgatacgaaa 27780 tattcgtagt actatcgata atttatttga aaattcataa gaaaagcaaa cgttacatga 27840 attgatgaaa caatacaaag acagataaag ccacgcacat ttaggatatt ggccgagatt 27900 actgaatatt gagtaagatc acggaatttc tgacaggagc atgtcttcaa ttcagcccaa 27960 atggcagttg aaatactcaa accgccccat atgcaggagc ggatcattca ttgtttgttt 28020 ggttgccttt gccaacatgg gagtccaagg ttgcggccgc ttaattaact actgtcactg 28080 aggccgtaga cgagtacgga ctgatctaac tagtgctagc ctcgaggtcg acacccagct 28140 ttcttgtaca aagtggttgc ggccgcttaa ttaaatttaa attcaattaa tgcaatcttg 28200 attttcaaca acgaaggtaa tggcgtaaaa gaaaaaatgt atgttattgt attgatcttt 28260 catgatgttg aagcgtgcca taatatgatg atgtataatt aaaatattaa ctgtcgcatt 28320 ttattgaaat ggcactgtta tttcaaccat atctttgatt ctgttacatg acacgactgc 28380 aagaagtaaa taatagacgc cgttgttaaa gaattgctat catatgtgcc taactagagg 28440 gaatttgagc gtcagaccta atcaaatatt acaaaatatc tcactctgtc gccagcaatg 28500 gtgtaatcag cgcagacaaa tggcgtaaag atcgcggaaa aacctccccg agtggcatga 28560 tagctgcctc tgtattgctg atttagtcag ccttatttga cttaagggtg ccctcgttag 28620 tgacaaattg ctttcaagga gacagccatg ccccacactt tgttgaaaaa caaattgcct 28680 ttggggagac ggtaaagcca gttgctcttc aataaggaat gtcgaggagg caatgtaacc 28740 gcctctggta gtacacttct ctaatccaaa aatcaatttg tattcaagat accgcaaaaa 28800 acttatggtt taaaccctgc aggactagtc cagaaggtaa ttatccaaga tgtagcatca 28860 agaatccaat gtttacggga aaaactatgg aagtattatg taagctcagc aagaagcaga 28920 tcaatatgcg gcacatatgc aacctatgtt caaaaatgaa gaatgtacag atacaagatc 28980 ctatactgcc agaatacgaa gaagaatacg tagaaattga aaaagaagaa ccaggcgaag 29040 aaaagaatct tgaagacgta agcactgacg acaacaatga aaagaagaag ataaggtcgg 29100 tgattgtgaa agagacatag aggacacatg taaggtggaa aatgtaaggg cggaaagtaa 29160 ccttatcaca aaggaatctt atcccccact acttatcctt ttatattttt ccgtgtcatt 29220 tttgcccttg agttttccta tataaggaac caagttcggc atttgtgaaa acaagaaaaa 29280 atttggtgta agctattttc tttgaagtac tgaggataca acttcagaga aatttgtaag 29340 tttgtaggta ccagatctgg atcccaaacc atgtctccgg agaggagacc agttgagatt 29400 aggccagcta cagcagctga tatggccgcg gtttgtgata tcgttaacca ttacattgag 29460 acgtctacag tgaactttag gacagagcca caaacaccac aagagtggat tgatgatcta 29520 gagaggttgc aagatagata cccttggttg gttgctgagg ttgagggtgt tgtggctggt 29580 attgcttacg ctgggccctg gaaggctagg aacgcttacg attggacagt tgagagtact 29640 gtttacgtgt cacataggca tcaaaggttg ggcctaggat ctacattgta cacacatttg 29700 cttaagtcta tggaggcgca aggttttaag tctgtggttg ctgttatagg ccttccaaac 29760 gatccatctg ttaggttgca tgaggctttg ggatacacag cccggggtac attgcgcgca 29820 gctggataca agcatggtgg atggcatgat gttggttttt ggcaaaggga ttttgagttg 29880 ccagctcctc caaggccagt taggccagtt acccaaatct gagtagttag cttaatcacc 29940 tagagctcga tcggcggcaa tagcttctta gcgccatccc gggttgatcc tatctgtgtt 30000 gaaatagttg cggtgggcaa ggctctcttt cagaaagaca ggcggccaaa ggaacccaag 30060 gtgaggtggg ctatggctct cagttccttg tggaagcgct tggtctaagg tgcagaggtg 30120 ttagcgggat gaagcaaaag tgtccgattg taacaagata tgttgatcct acgtaaggat 30180 attaaagtat gtattcatca ctaatataat cagtgtattc caatatgtac tacgatttcc 30240 aatgtcttta ttgtcgccgt atgtaatcgg cgtcacaaaa taatccccgg tgactttctt 30300 ttaatccagg atgaaataat atgttattat aatttttgcg atttggtccg ttataggaat 30360 tgaagtgtgc ttgaggtcgg tcgccaccac tcccatttca taattttaca tgtatttgaa 30420 aaataaaaat ttatggtatt caatttaaac acgtatactt gtaaagaatg atatcttgaa 30480 agaaatatag tttaaatatt tattgataaa ataacaagtc aggtattata gtccaagcaa 30540 aaacataaat ttattgatgc aagtttaaat tcagaaatat ttcaataact gattatatca 30600 gctggtacat tgccgtagat gaaagactga gtgcgatatt atggtgtaat acataggaat 30660 tcgtttaaac gatctgcgtc taattttcgg tccaacttgc acaggaaaga cgtcgaccgc 30720 ggtagctctt gcccagcaga ctgggcttcc agtcctttcg ctcgatcggg tccaatgttg 30780 tcctcagctg tgaaccggaa gcggacgacc aacagtggaa gaactgaaag gaacgagccg 30840 tctatacctt gatgatcggc ctctggtgaa gggtatcatc gcagccaagc aagctcatga 30900 aaggctgatg ggggaggtgt ataattatga ggcccacggc gggcttattc tttagggagg 30960 atctatctcg ttgctcaagt gcatggcgca aagcagttat tggagtgcgg attttcgttg 31020 gcatattatt cgccacgagt tagcagacga agagaccttc atgaacgtgg ccaaggccag 31080 agttaagcag atgttacgcc ctgctgcagg cctttctatt atccaatagt tggttgatct 31140 ttggaaagag cctcggctga ggcccatact gaaagagatc gatggatatc gatatgccat 31200 gttgtttgct agccagaacc agatcacatc cgatatgcta ttgcagcttg acgcagatat 31260 ggaggataag ttgattcatg ggatcgctca ggagtagctc atccatgcac gccgacaaga 31320 acagaaattc cgtcgagtta acgcagccgc ttacgacgga ttcgaaggtc atccattcgg 31380 aatgtattag tttgcaccag ctccgcgtca cacctgtctt catttgaata agatgttagc 31440 aattgttttt agctttgtct tgttgtggca gggcggcaag tgcttcagac atcattctgt 31500 tttcaaattt tatgctggag aacagcttct taattccttt ggaaataata gactgcgtct 31560 taaaattcag atgtctggat atagatatga ttgtaaaata acctatttaa gtgtcattta 31620 gaacataagt tttatgaatg ttcttccatt ttcgtcatcg aacgaataag agtaaataca 31680 ccttttttaa cattacaaat aagttcttat acgttgttta tacaccggga atcatttcca 31740 ttattttcgc gcaaaagtca cggatattcg tgaaagcgac ataaactgcg aaatttgcgg 31800 ggagtgtctt gagtttgcct cgaggctagc gcatgcacat agacacacac atcatctcat 31860 tgatgcttgg taataattgt cattagattg tttttatgca tagatgcact cgaaatcagc 31920 caattttaga caagtatcaa acggatgtga cttcagtaca ttaaaaacgt ccgcaatgtg 31980 ttattaagtt gtctaagcgt caatttgatt tacaattgaa tatatcctgc cccagccagc 32040 caacagctcg atttacaatt gaatatatcc tgccggccgg cccacgcgtg tcgaggaatt 32100 ctgatctggc ccccatttgg acgtgaatgt agacacgtcg aaataaagat ttccgaatta 32160 gaataatttg tttattgctt tcgcctataa atacgacgga tcgtaatttg tcgttttatc 32220 aaaatgtact ttcattttat aataacgctg cggacatcta catttttgaa ttgaaaaaaa 32280 attggtaatt actctttctt tttctccata ttgaccatca tactcattgc tgatccatgt 32340 agatttcccg gacatgaagc catttacaat tgaatatatc ctgccgccgc tgccgctttg 32400 cacccggtgg agcttgcatg ttggtttcta cgcagaactg agccggttag gcagataatt 32460 tccattgaga actgagccat gtgcaccttc cccccaacac ggtgagcgac ggggcaacgg 32520 agtgatccac atgggacttt taaacatcat ccgtcggatg gcgttgcgag agaagcagtc 32580 gatccgtgag atcagccgac gcaccgggca ggcgcgcaac acgatcgcaa agtatttgaa 32640 cgcaggtaca atcgagccga cgttcacgcg gaacgaccaa gcaagcttgg ctgccatttt 32700 tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt 32760 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc 32820 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa 32880 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc 32940 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct 33000 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc 33060 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg 33120 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc 33180 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc 33240 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc 33300 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc 33360 cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggg tcgacggatc ttttccgctg 33420 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac 33480 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc 33540 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc 33600 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg 33660 ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca 33720 gcccacctat caaggtgtac tgccttccag acgaacgaag agcgattgag gaaaaggcgg 33780 cggcggccgg catgagcctg tcggcctacc tgctggccgt cggccagggc tacaaaatca 33840 cgggcgtcgt ggactatgag cacgtccgcg agctggcccg catcaatggc gacctgggcc 33900 gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg cggttcggtg 33960 atgccacgat cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag cttggcaagg 34020 tcatgatggg cgtggtccgc ccgagggcag agccatgact tttttagccg ctaaaacggc 34080 cggggggtgc gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa gagcgacttc 34140 gcggagctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag 34200 acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca 34260 ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa 34320 ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg 34380 gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc 34440 gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac 34500 agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt 34560 cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact 34620 atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc 34680 aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg 34740 ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct 34800 gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt 34860 ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat 34920 gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg 34980 atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat 35040 tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc 35100 gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt 35160 ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct 35220 ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga 35280 cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc 35340 cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa 35400 gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg 35460 gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagccagc 35520 gctttactgg catttcagga acaagcgggc actgctcgac gcacttgctt cgctcagtat 35580 cgctcgggac gcacggcgcg ctctacgaac tgccgataaa cagaggatta aaattgacaa 35640 ttgtgattaa ggctcagatt cgacggcttg gagcggccga cgtgcaggat ttccgcgaga 35700 tccgattgtc ggccctgaag aaagctccag agatgttcgg gtccgtttac gagcacgagg 35760 agaaaaagcc catggaggcg ttcgctgaac ggttgcgaga tgccgtggca ttcggcgcct 35820 acatcgacgg cgagatcatt gggctgtcgg tcttcaaaca ggaggacggc cccaaggacg 35880 ctcacaaggc gcatctgtcc ggcgttttcg tggagcccga acagcgaggc cgaggggtcg 35940 ccggtatgct gctgcgggcg ttgccggcgg gtttattgct cgtgatgatc gtccgacaga 36000 ttccaacggg aatctggtgg atgcgcatct tcatcctcgg cgcacttaat atttcgctat 36060 tctggagctt gttgtttatt tcggtctacc gcctgccggg cggggtcgcg gcgacggtag 36120 gcgctgtgca gccgctgatg gtcgtgttca tctctgccgc tctgctaggt agcccgatac 36180 gattgatggc ggtcctgggg gctatttgcg gaactgcggg cgtggcgctg ttggtgttga 36240 caccaaacgc agcgctagat cctgtcggcg tcgcagcggg cctggcgggg gcggtttcca 36300 tggcgttcgg aaccgtgctg acccgcaagt ggcaacctcc cgtgcctctg ctcaccttta 36360 ccgcctggca actggcggcc ggaggacttc tgctcgttcc agtagcttta gtgtttgatc 36420 cgccaatccc gatgcctaca ggaaccaatg ttctcggcct ggcgtggctc ggcctgatcg 36480 gagcgggttt aacctacttc ctttggttcc gggggatctc gcgactcgaa cctacagttg 36540 tttccttact gggctttctc agcccccgag cgcttagtgg gaatttgtac cccttatcga 36600 accgggagca caggatgacg cctaacaatt cattcaagcc gacaccgctt cgcggcgcgg 36660 cttaattcag gagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact 36720 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt 36780 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac 36840 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac 36900 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca 36960 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca 37020 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat 37080 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact 37140 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg 37200 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat 37260 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat 37320 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctaggcagg cttatcttgg 37380 acaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa 37440 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg 37500 cttcgcggcg cggcttaact caagcgttag agagctgggg aagactatgc gcgatctgtt 37560 gaaggtggtt ctaagcctcg tcttgcgatg gcatttcgat ccattcccat tccgcgctca 37620 agatggcttc ccctcggcag ttcatcaggg ctaaatcaat ctagccgact tgtccggtga 37680 aatgggctgc actccaacag aaacaatcaa acaaacatac acagcgactt attcacacga 37740 gctcaaatta caacggtata tatcctgcca gtcagcatca tcacaccaaa agttaggccc 37800 gaatagtttg aaattagaaa gctcgcaatt gaggtctaca ggccaaattc gctcttagcc 37860 gtacaatatt actcaccgga tcctaaccgg tttaattaag gcgcgccatg cccgggcaag 37920 cggccgcaca agttt 37935 <210> 61 <211> 38239 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB109508 <400> 61 gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat ctcagcatgt 60 gcgtagcacg cggccgcctc ccagtatcat tatagtgaaa gttttggctc tctcgccggt 120 ggttttttac ctctatttaa aggggttttc cacctaaaaa ttctggtatc attctcactt 180 tacttgttac tttaatttct cataatcttt ggttgaaatt atcacgcttc cgcacacgat 240 atccctacaa atttattatt tgttaaacat tttcaaaccg cataaaattt tatgaagtcc 300 cgtctatctt taatgtagtc taacattttc atattgaaat atataattta cttaatttta 360 gcgttggtag aaagcataat gatttattct tattcttctt catataaatg tttaatatac 420 aatataaaca aattctttac cttaagaagg atttcccatt ttatatttta aaaatatatt 480 tatcaaatat ttttcaacca cgtaaatctc ataataataa gttgtttcaa aagtaataaa 540 atttaactcc ataatttttt tattcgactg atcttaaagc aacacccagt gacacaacta 600 gccatttttt tctttgaata aaaaaatcca attatcattg tatttttttt atacaatgaa 660 aatttcacca aacaatgatt tgtggtattt ctgaagcaag tcatgttatg caaaattcta 720 taattcccat ttgacactac ggaagtaact gaagatctgc ttttacatgc gagacacatc 780 ttctaaagta attttaataa tagttactat attcaagatt tcatatatca aatactcaat 840 attacttcta aaaaattaat tagatataat taaaatatta cttttttaat tttaagttta 900 attgttgaat ttgtgactat tgatttatta ttctactatg tttaaattgt tttatagata 960 gtttaaagta aatataagta atgtagtaga gtgttagagt gttaccctaa accataaact 1020 ataagattta tggtggacta attttcatat atttcttatt gcttttacct tttcttggta 1080 tgtaagtccg taactggaat tactgtgggt tgccatgaca ctctgtggtc ttttggttca 1140 tgcatggatc ttgcgcaaga aaaagacaaa gaacaaagaa aaaagacaaa acagagagac 1200 aaaacgcaat cacacaacca actcaaatta gtcactggct gatcaagatc gccgcgtcca 1260 tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca tgcactttaa atggctcacc 1320 catctcaacc cacacacaaa cacattgcct ttttcttcat catcaccaca accacctgta 1380 tatattcatt ctcttccgcc acctcaattt cttcacttca acacacgtca acctgcatat 1440 gcgtgtcatc ccatgcccaa atctccatgc atgttccaac caccttctct cttatataat 1500 acctataaat acctctaata tcactcactt ctttcatcat ccatccatcc agagtactac 1560 tactctacta ctataatacc ccaacccaac tcatattcaa tactactcta ggatccacaa 1620 ccaaaaatgg cagcgaggct gcaagagcag aaaggaggtg agatggacac gaggattgcg 1680 atcataggca tgagcgccat acttccttgt ggaaccacgg tgagagagtc atgggagacc 1740 ataagggctg gcattgattg cctgtctgat cttcctgagg atcgtgttga tgtgactgcg 1800 tactttgacc cagtcaagac cacaaaggac aaaatctact gcaagagagg aggtttcatt 1860 cctgagtatg actttgatgc gcgtgagttt ggtctcaaca tgttccagat ggaagattct 1920 gatgcgaatc agaccatctc acttctcaag gtcaaagaag cacttcaaga tgctgggatt 1980 gatgcacttg gaaaggagaa gaaaaacata ggatgcgtgc ttggcattgg aggtggacag 2040 aagagttcac atgagttcta cagcagactc aactatgtgg ttgtggagaa agtcttgaga 2100 aagatgggga tgccagagga agatgtcaaa gtggctgtgg agaagtacaa agcgaacttc 2160 ccagaatgga gacttgactc tttccctgga ttccttggga atgtcactgc tggcagatgc 2220 accaacacat tcaacttgga tggcatgaac tgcgttgtgg atgctgcatg tgcaagctcc 2280 ttgatagcag tcaaggttgc gattgatgaa ctgctctatg gtgactgcga catgatggtc 2340 actggtgcaa cctgcacaga caactccatt gggatgtaca tggctttctc aaagacacct 2400 gtgttcagca cggacccaag tgtgagagcg tatgatgaga aaacgaaagg gatgctcatt 2460 ggtgagggat cagccatgtt ggtgctcaaa cgttatgcag atgcagtgag ggatggtgat 2520 gagattcatg ctgtgattcg tggttgtgca agctcaagtg atggcaaagc agctggcatc 2580 tacactccca caatcagtgg tcaagaggaa gccttgagac gtgcatacaa tcgtgcctgc 2640 gttgaccctg ccactgtcac tttggttgag ggtcatggga ctggcactcc agttggagat 2700 aggattgagt tgactgcgtt gaggaacttg tttgacaaag cgtatggtga agggaacaca 2760 gagaaagttg cagttggaag tatcaagtcc agtattggtc atttgaaggc tgttgctggt 2820 cttgctggaa tgatcaaggt catcatggct ctcaaacaca agactttgcc tggaacgatc 2880 aatgtggaca atccacccaa tctctatgac aacactccca tcaatgagtc atccctctac 2940 atcaacacga tgaacagacc ctggttccct ccacctggtg ttccgagacg tgctggaata 3000 tccagctttg gctttggagg tgccaactac catgcagtgt tggaagaggc tgaaccagag 3060 cacacgacag cgtacagact caacaaacgt ccacaaccgg tgttgatgat ggctgcaact 3120 cctgctgcgt tgcagagcct ttgtgaagct cagctgaaag agtttgaagc tgcgatcaag 3180 gagaatgaga cagtcaagaa tactgcctac atcaagtgtg tcaagtttgg agaacagttc 3240 aagttccctg ggagcatacc tgcgaccaat gcgagactgg gattcttggt caaagatgcg 3300 gaggatgcgt gctccacgct tcgtgctata tgtgctcagt ttgccaaaga tgtgaccaaa 3360 gaagcgtgga ggcttccgag agaaggagtt tctttccgtg ccaaaggcat tgccaccaat 3420 ggtgctgttg ctgcactctt ctctggacaa ggtgctcagt acacacacat gttcagtgaa 3480 gttgcgatga actggcctca gttcagacag tccattgctg caatggacgc tgcacagtcc 3540 aaggttgctg gaagtgacaa agactttgaa agagtctctc aagtcctgta cccaaggaaa 3600 ccgtacgaac gtgagccaga acaagatcac aagaaaatct cactcacagc atacagccag 3660 ccgtcaaccc tggcatgtgc tcttggtgcc tttgagattt tcaaggaagc tggattcact 3720 ccagactttg cagctggaca cagcttgggt gagtttgctg cgctctatgc agctggttgt 3780 gttgacagag atgagttgtt tgaacttgtt tgcagaaggg caaggatcat gggaggcaaa 3840 gatgcacctg ccacacccaa aggttgcatg gcagctgtca taggacccaa cgcagagaac 3900 atcaaggttc aagctgccaa cgtctggctt ggaaactcaa actctccgtc tcagactgtc 3960 attactgggt ctgttgaagg gattcaagct gaaagtgcca gattgcagaa ggaagggttc 4020 agagttgtgc ctcttgcttg tgagtctgcc ttccattctc cacagatgga aaatgccagc 4080 tcagctttca aagatgtcat atccaaggtt tccttccgta cacccaaagc agaaacaaag 4140 ctcttcagca atgtttctgg agagacctat ccgacggatg ccagagagat gctcactcaa 4200 cacatgacct catctgtgaa gttcctgacc caagtgagga acatgcacca agctggtgcg 4260 agaatctttg ttgagtttgg accgaaacaa gttctcagca aacttgtttc tgaaaccctc 4320 aaagatgacc cgtctgttgt cacagtgtct gtgaacccag cttctggcac ggactctgac 4380 atacagttga gggatgctgc agttcagctg gttgtggctg gtgtcaatct tcaagggttt 4440 gacaagtggg atgctcctga tgccacgagg atgcaagcca tcaagaaaaa gagaaccaca 4500 ctcagacttt cagctgccac ctatgtcagt gacaagacca agaaagtgag ggatgctgcc 4560 atgaacgacg gaagatgtgt cacatacctc aagggtgctg caccactcat caaggctcct 4620 gaacctgtgg ttgacgaagc tgccaagagg gaggctgaaa ggcttcagaa ggagcttcaa 4680 gacgctcaga ggcagcttga cgacgccaaa cgtgcagctg cagaagccaa ctccaaactt 4740 gctgctgcca aggaggaagc caagactgct gcagcttcag ccaagcctgc agttgacact 4800 gctgttgtgg agaaacatcg tgccatcctc aagtccatgc tggctgagct ggatggttat 4860 ggaagtgttg atgcttcttc ccttcagcaa cagcaacagc aacagactgc tccggcgcct 4920 gttaaagccg ctgcccctgc cgctcccgtt gcaagtgctc ctgctcccgc cgtgtccaac 4980 gagttgcttg agaaagctga gacagttgtg atggaggttc tcgctgccaa gactggatac 5040 gaaacagaca tgattgaggc ggacatggag cttgagactg agttgggaat tgacagcatc 5100 aagagggttg aaattctttc tgaagtccaa gctatgctta acgttgaggc gaaagatgtt 5160 gatgctctct ctaggacgag aacagtgggt gaagttgtca acgctatgaa ggcagaaatc 5220 gctggttctt ccgctccggc gccggcagct gccgcacctg caccggctaa agcggctcca 5280 gctgcagctg caccagctgt ttctaatgag ctgctcgaaa aggcagaaac tgttgtgatg 5340 gaagtccttg ctgccaaaac tggctacgaa actgacatga tagaatcaga catggagctt 5400 gagacggagc ttggaattga ttccatcaag cgtgtggaaa tcctcagtga agtccaagcc 5460 atgctcaatg tggaagctaa ggatgtggat gctctctcaa ggaccagaac tgtcggtgag 5520 gttgtcaatg ccatgaaggc tgagatagct ggaggctccg ctcctgcgcc ggccgctgcc 5580 gctcctggtc ccgctgccgc ggctccagct ccagctgcag ccgctccggc cgtctcaaac 5640 gaactccttg aaaaagctga gaccgtcgtg atggaggtct tggcagctaa aaccggctat 5700 gagactgaca tgattgagtc tgacatggaa ttggagacag aacttgggat agacagcatc 5760 aaacgtgttg aaatcctcag tgaggtccaa gctatgctga acgtggaagc gaaggatgtt 5820 gatgctctgt ccagaacgag gaccgttggt gaggttgtgg atgccatgaa ggctgaaatt 5880 gctggagggt ctgcgcccgc gccagctgca gccgctcctg cacccgcagc cgcggcacct 5940 gctccagctg cacctgcacc tgccgtttct tcagaactct tggagaaggc tgagacggtt 6000 gtgatggagg ttttggctgc aaagactggc tatgagaccg acatgattga atctgatatg 6060 gaattggaga cagagcttgg gatcgattcc atcaaaagag tggagatcct gagtgaagtc 6120 caagccatgc tcaatgttga agctaaggat gttgatgcac tttcaagaac aaggacagtg 6180 ggtgaggttg tggatgccat gaaggcggag attgctggag ggagcgcgcc tgcgccagct 6240 gccgcggcac ctgcaccggc agccgcagct ccagctccag ctgcaccagc accggccgcg 6300 cccgcgccag ctgtttcatc cgaactcctg gaaaaggccg agacggttgt gatggaggtg 6360 ctcgcagcta agactggtta cgagaccgac atgattgaaa gtgacatgga gcttgaaacc 6420 gagttgggaa tagatagcat caaacgtgtt gaaatcttgt ctgaggtcca agctatgttg 6480 aacgtggagg caaaagatgt cgatgcgctt tcaagaacca gaacagtcgg tgaggtcgtg 6540 gacgccatga aggctgaaat tgccggaagc tctgctagtg cgccagctgc agccgcacct 6600 gcacctgctg ccgcggcacc tgctccagct gcagccgctc ctgccgtcag caatgagttg 6660 ctggagaaag ccgaaacagt tgtgatggaa gtccttgctg caaaaactgg ttacgaaacg 6720 gacatgattg agtcagacat ggaattggag accgaacttg gaatagattc catcaagaga 6780 gtggagatcc tctctgaggt gcaagccatg ctcaacgttg aggctaaaga tgttgatgca 6840 ctcagtagga caagaactgt gggggaagtt gtcgatgcga tgaaggctga gattgctgga 6900 ggctctgctc ctgcgccggc agctgccgca cctgcacccg cagccgcggc accagctgtc 6960 agcaacgaat tgcttgagaa ggctgaaaca gttgtcatgg aagtgctggc tgcaaaaacc 7020 ggctatgaga ctgacatgat tgagtctgac atggaacttg agacggaatt gggcattgac 7080 agcatcaagc gtgttgagat cctcagtgaa gtccaagcaa tgctcaatgt ggaggctaag 7140 gatgtggatg ccctctcaag gaccagaaca gttggtgagg tcgttgatgc gatgaaggcg 7200 gagatcgctg gaagttccgc gcctgcgcca gctgcagccg ctcctgcacc cgcagccgcg 7260 gcaccggctc cagctgcagc cgcaccggcc gtctcctcag aactcctgga aaaggctgag 7320 acagttgtga tggaagtcct tgctgcgaaa actggctatg agaccgacat gattgaatct 7380 gacatggaac tggagaccga gcttggaatt gactccatca aacgtgttga aatcctctct 7440 gaggttcaag cgatgttgaa tgtggaggcc aaagatgttg atgctctttc cagaacaagg 7500 acggtgggag aggtggttga tgccatgaag gctgagatag ctgggggttc tgctcctgcg 7560 ccagctgcag ccgctcctgc tcccgctgcc gcggcaccag ctgtcagcaa cgaactcctt 7620 gaaaaagctg aaactgttgt gatggaggtt ttggctgcaa agactggata cgagactgac 7680 atgatagagt cagacatgga gttggaaaca gagcttggga ttgatagcat caagcgtgtg 7740 gaaatccttt ctgaggttca agccatgctg aacgttgagg ccaaagatgt cgatgctttg 7800 tcaaggacca gaacggttgg agaagtggtc gatgccatga aggctgagat agctggtgga 7860 tcagcccctg ctccggctgc agccgctcct gcaagtgccg gtgctgcacc agctgtgaag 7920 attgattcag ttcatggtgc tgactgtgac gatttgtcac tgatgcacgc aaaggtggtt 7980 gacatcagaa ggccagatga actgattctg gaaagaccgg agaaccgtcc agttcttgtc 8040 gttgacgatg gctcagagtt gacacttgct cttgtgaggg tgttgggagc ctgtgcagtt 8100 gtcttgacct ttgagggatt gcagttggct cagagagctg gtgcagctgc cattcgtcat 8160 gttctggcta aggacctttc agcagaatca gctgagaagg ccatcaaaga ggctgagcaa 8220 agatttggag cgttgggagg cttcataagc cagcaagcag aaaggtttga accagctgag 8280 atactgggtt tcacactcat gtgtgcgaag tttgccaaag cctctctctg cactgctgtg 8340 gctggaggtc gtcctgcctt catcggtgtg gctaggcttg acggcagact tggattcacc 8400 tctcaaggca cttctgacgc actcaagaga gcacagcgtg gagcgatctt tggactctgc 8460 aagacaatag ggttggagtg gtcagaatct gatgtgttca gccgtggtgt ggacattgct 8520 caagggatgc acccagaaga tgctgcagtt gccatagtta gggagatggc gtgtgctgac 8580 atcagaatca gagaggttgg gattggagca aaccagcaaa gatgcacaat cagagctgcc 8640 aagttggaga ctgggaaccc tcagagacag atagcgaaag acgatgtgct tctggtttct 8700 ggaggtgctc gtgggatcac acctctttgc atccgtgaga tcacgcgtca gattgctgga 8760 ggcaagtaca ttctgttggg tcgtagcaaa gtttctgcgt ctgaaccagc atggtgtgct 8820 ggcatcactg atgagaaggc tgtgcagaaa gctgccaccc aagagttgaa gagggccttc 8880 agtgctggtg aaggacccaa acccactcca agggctgtca cgaaacttgt gggatctgtt 8940 cttggtgctc gtgaagtgag aagctccatt gctgccattg aagctctggg tggcaaagcg 9000 atctacagct cttgtgatgt gaactctgct gcagatgttg ccaaagcagt tagggatgca 9060 gagtcccagc ttggtgccag agttagtgga atcgttcacg cttctggtgt gctccgtgat 9120 cgtctcatag agaagaaact ccctgacgag tttgatgctg tctttgggac caaagtcact 9180 gggttggaga atcttctggc tgcagttgat cgtgccaacc tcaaacacat ggttctcttc 9240 agctcccttg ctggtttcca cggcaatgtt ggtcagtctg actatgcgat ggcaaacgaa 9300 gctctcaaca agatgggttt ggaacttgcg aaggatgtca gtgtcaagtc catctgcttt 9360 ggtccctggg atggtgggat ggtgacacct caactcaaga aacagtttca agaaatggga 9420 gttcagatca ttcctcgtga aggaggtgct gatacagttg cgaggattgt gttgggatca 9480 agtcctgctg agatccttgt tggaaactgg aggacaccat ccaagaaagt tggaagtgac 9540 acgatcaccc tgcatagaaa gatcagtgcc aagtccaatc ccttcttgga ggatcatgtc 9600 atacaaggca gaagggtgtt gccaatgacc cttgctattg ggagccttgc tgagacctgc 9660 cttggactct tccctggcta ctctctctgg gccatagatg acgcacagct cttcaaaggt 9720 gtcactgttg atggagacgt caactgtgaa gtgaccctca caccgtccac tgctccgagt 9780 gggagggtga atgtccaagc gacgctcaag acgttctcaa gtggaaaact tgttccagcc 9840 tacagagctg tcatagtgtt gtccaaccaa ggtgctccac ctgccaatgc aaccatgcag 9900 cctccgagcc ttgatgcaga tcctgctttg caagggtcag tctatgatgg caagactctc 9960 tttcatggtc ctgcgttcag aggcattgat gacgttctca gctgcaccaa gagccagctt 10020 gttgccaagt gttctgctgt tcctggatca gatgcagcca gaggagagtt tgcaactgac 10080 actgatgcac atgatccctt tgtcaatgat cttgcctttc aagcaatgtt ggtctgggtg 10140 aggagaacgc tgggacaagc tgcactcccg aactccattc agaggatagt ccaacacaga 10200 ccggtcccac aagacaaacc cttctacatc accctcagat ccaatcagag tggtggacat 10260 agccagcaca aacatgcact tcagttccac aacgaacaag gtgatctctt cattgatgtt 10320 caagcctctg tcattgcgac agactcactt gcgttctgag tagttagctt aatcacttag 10380 gtcaccagta tgaactaaaa tgcatgtagg tgtaagagct catggagagc atggaatatt 10440 gtatccgacc atgtaacagt ataataactg agctccatct cacttcttct atgaataaac 10500 aaaggatgtt atgatatatt aacactctat ctatgcacct tattgttcta tgataaattt 10560 cctcttatta ttataaatca tctgaatcgt gacggcttat ggaatgcttc aaatagtaca 10620 aaaacaaatg tgtactataa gactttctaa acaattctaa ctttagcatt gtgaacgaga 10680 cataagtgtt aagaagacat aacaattata atggaagaag tttgtctcca tttatatatt 10740 atatattacc cacttatgta ttatattagg atgttaagga gacataacaa ttataaagag 10800 agaagtttgt atccatttat atattatata ctacccattt atatattata cttatccact 10860 tatttaatgt ctttataagg tttgatccat gatatttcta atattttagt tgatatgtat 10920 atgaaaaggt actatttgaa ctctcttact ctgtataaag gttggatcat ccttaaagtg 10980 ggtctattta attttattgc ttcttacaga taaaaaaaaa attatgagtt ggtttgataa 11040 aatattgaag gatttaaaat aataataaat aataaataac atataatata tgtatataaa 11100 tttattataa tataacattt atctataaaa aagtaaatat tgtcataaat ctatacaatc 11160 gtttagcctt gctggaacga atctcaatta tttaaacgag agtaaacata tttgactttt 11220 tggttattta acaaattatt atttaacact atatgaaatt tttttttttt atcagcaaag 11280 aataaaatta aattaagaag gacaatggtg tcccaatcct tatacaacca acttccacaa 11340 gaaagtcaag tcagagacaa caaaaaaaca agcaaaggaa attttttaat ttgagttgtc 11400 ttgtttgctg cataatttat gcagtaaaac actacacata acccttttag cagtagagca 11460 atggttgacc gtgtgcttag cttcttttat tttatttttt tatcagcaaa gaataaataa 11520 aataaaatga gacacttcag ggatgtttca acccttatac aaaaccccaa aaacaagttt 11580 cctagcaccc taccaacgaa ttcgcggccg cttaattaag atgagtgata ctcaggactc 11640 aggactcact ctgctgatca ctagtgctag cctcgaggtc gacacaactt tgtatacaaa 11700 agttgtctag acctaggtta attaagcggc cgcagcttat ttctcttttc gatactctaa 11760 ttgagccgtg cgctctatct agaccaatta gaattgatgg agctctaaag gttgctggct 11820 gttttcttgt tcatatgatt aacttctaaa cttgtgtata aatattctct gaaagtgctt 11880 cttttggcat atgtaggttg ggcaaaaacg aggaagattg cttctcaatt tggaagatga 11940 tgaacagccg aagaagaaaa taagaatagg cagtcctgct actcaatgga tctcagtcta 12000 taacggtcgt cgtcccatga aacagaggta acacattttt tgcatataca ctttgatagt 12060 tcctcactaa ctgtgtaatc ttttggtaga tatcactaca atgttggaga gacaaggctg 12120 cgccagcata tacagaaggg aaatgaagat ggccttttga ttagctgtgt agcatcagca 12180 gctaatctct gggctctcat catggatgct ggaactggat tcacttctca agtttatgag 12240 ttgtcaccgg tcttcctaca caaggtaata atcagttgaa gcaattaaga atcaatctga 12300 tttgtagtaa actaagaaga acttacctta tgatttcccc gcaggactgg attatggaac 12360 aatgggaaaa gaactactat ataagctcca tagcgggttc agataacggg agctctttag 12420 ttgttatgtc aaaaggttag tgtttagtga ataataaact tattatcaaa aagtcttcat 12480 tgacttattt atatacttgt tgtgaattgg taggaactac ttattctcag cagtcataca 12540 aagtgagtga ctcatttcca ttcaagtgga taaataagaa atggaaagaa gattttcatg 12600 taacctccat gacaactgct ggtaatcgtt ggggtgtggt aatgtcgagg aactctggct 12660 tctctgatca ggtaggtttt tgtctcttat tgtctggtgt ttttattttc ccctgatagt 12720 ctaatatgat aaactctgcg ttgtgaaagg tggtggagct tgactttttg tacccaagcg 12780 atgggataca taggaggtgg gagaatgggt atagaataac atcaatggca gcaactgcgg 12840 atcaagcagc tttcatatta agcataccaa agcgtaagat ggtggatgaa actcaagaga 12900 ctctccgcac caccgccttt ccaagtactc atgtcaaggt tggtttcttt agctttgaac 12960 acagatttgg atctttttgt tttgtttcca tatacatagg acctgagagc ttttggttga 13020 attttttttt tttcaggaca aatgggcgaa gaatctgtac attgcatcaa tatgctatgg 13080 caggacagtg tgctgatgat acacacttaa gcatcatgtg ttgtgttaga aagccgaaga 13140 caattggagc gagcctcagg gtcgtcataa taccaatcaa agacgtaaaa ccagacgcag 13200 tctctttggt tgaatgtgat gaaagggatg tgtcttggta tgtatgtacg agtaacaaaa 13260 gagaagatgc aattgagtag tagaaagatt tgagagcttt ttaaagccct tcaagtgtgt 13320 gcttttatct tattgatatc atccatttgc gttgtttaat gcgtctttag atatgtttct 13380 gtttctttct cagtgtctga atatctgata agtgcaatgt gagaaagcca caccaaacca 13440 aaatattcaa atcttatatt tttaataatg tcgaatcact cggagttgcc accttctgtg 13500 ccaattgtgc tgaatctatc acactaaaaa aaacatttct tcaaggtaat gacttgtgga 13560 ctatgttctg aattctcatt aagtttttat tttttgaagt ttaagttttt accttctttt 13620 ttgaaaaata tcgttcataa gatgtcacgc caggacatga gctacacatc acatattagc 13680 atgcagatgc ggacgatttg tcactcactt caaacaccta aaagagcttc tctctcacag 13740 cacacacaca tatgcatgca atatttacac gtgatcgcca tgcaaatctc cattctcacc 13800 tataaattag aggctcggct tcacttttta ctcaaaccaa aactcatcac tacaagatcc 13860 acaaccaaaa atggctgcaa ggaacgtgag tgcggctcat gagatgcatg acgagaagag 13920 gatagctgtt gtgggcatgg ctgttcagta tgctggatgc aaaacgaaag acgagttctg 13980 ggaagttctc atgaatggaa aagttgagag caaagtgatc agcgacaaac gtcttggttc 14040 aaattaccgt gctgaacact acaaagctga aaggagcaag tatgctgaca ccttctgcaa 14100 tgagacatat ggaactcttg atgagaacga aatagacaat gagcatgaac tccttttgaa 14160 ccttgccaaa caagccttgg ctgaaacatc tgtcaaagat tcaacaagat gtggcatagt 14220 ttctggatgc ctcagcttcc cgatggacaa cctccaaggt gaactcttga atgtgtacca 14280 gaaccatgtt gagaagaaac ttggtgccag agttttcaaa gatgcctctc actggtcaga 14340 aagagaacag tccaacaaac cagaggctgg tgacagaaga atcttcatgg accctgcttc 14400 ctttgttgct gaggaactca atcttggcgc tctccactac tctgttgatg ctgcctgtgc 14460 cactgcgctc tatgtgctca gactggcgca agatcacttg gtctctggtg cagctgatgt 14520 gatgctttgt ggtgcaacat gcctgccgga gccgtttttc atactctctg ggttctcaac 14580 cttccaagcc atgcctgttg gaacgggtca gaatgtgtcc atgccgctgc acaaagattc 14640 tcaaggattg acccctggtg aaggaggttc catcatggtc ctcaaacgtt tggatgacgc 14700 aatcagagat ggagatcaca tctatggaac acttttggga gccaatgtca gcaacagtgg 14760 cactggtctg ccacttaaac cccttttgcc gtcagagaaa aagtgcctca tggacaccta 14820 cacacgtatc aatgtgcatc cccacaagat tcagtatgtt gaatgccatg caactgggac 14880 tccacaaggt gatcgtgtgg aaattgatgc tgtgaaggct tgctttgaag gcaaagtccc 14940 acgttttgga accacaaaag ggaactttgg acacacattg gtggcagctg ggtttgctgg 15000 catgtgcaaa gtgcttctct ccatgaagca cgggatcatt ccacccactc cgggaataga 15060 tgacgaaacg aagatggacc ctctggttgt ctctggtgag gccattccct ggccagaaac 15120 gaatggagaa ccgaagcgtg ctggtctctc agcgtttgga tttggtggga ccaatgctca 15180 tgctgtcttt gaggaacatg acccaagcaa tgctgcatgc actggccatg actccatttc 15240 tgctctctct gcacgttgtg gtggtgagtc aaacatgaga atagcaatca ctgggatgga 15300 tgccactttt ggtgctttga aaggattgga tgcttttgaa agggccatct acactggtgc 15360 tcatggtgct ataccactcc cagaaaagag atggagattc ctggggaaag acaaggactt 15420 ccttgatctt tgtggagtca aagcaacacc gcatggctgt tacattgaag atgttgaagt 15480 tgatttccag aggctgagga ctccaatgac tcctgaagat atgctcttgc ctcaacagct 15540 tctggctgtc accacaattg atagggccat attggactct ggcatgaaga aaggtggaaa 15600 tgtggctgtc tttgtggggt tggggaccga tcttgaactc taccgtcacc gtgcaagggt 15660 tgctctcaag gagagagtga ggcctgaagc cagcaagaaa ctcaatgaca tgatgcagta 15720 catcaatgat tgtgggacaa gcacttctta caccagctac ataggcaact tggttgcaac 15780 aagggtcagt tcacaatggg gcttcacggg accttccttc acaatcacag aaggcaacaa 15840 ttctgtgtac agatgtgctg aacttggcaa atacctcttg gaaacgggag aggttgatgg 15900 ggttgtggtt gcgggtgttg atctctgtgg gagtgctgag aacctttacg tcaagtcaag 15960 gcgtttcaag gtgtctacat ctgacacccc gagagccagc tttgatgcag ctgctgatgg 16020 ttactttgtt ggagagggct gtggtgcatt tgtgctcaag cgtgagacaa gctgcaccaa 16080 ggatgacaga atctatgctt gcatggatgc catagttcct ggaaatgttc catctgcatg 16140 tttgagagag gcattggatc aagccagagt caagcctgga gacatcgaga tgttggagct 16200 ttctgctgat tcagcgaggc atttgaagga cccgtctgtt ctcccaaagg aactcactgc 16260 tgaggaagag attggcggac ttcagacgat tcttcgtgac gatgacaagc tcccaaggaa 16320 tgttgcaact ggaagtgtca aggccactgt tggtgacact ggttatgctt ctggtgctgc 16380 cagtttgatt aaggctgcgc tttgcatcta caaccgttac ctgcccagca atggagatga 16440 ctgggatgaa cctgcacctg aagcaccctg ggacagcaca ctctttgcgt gccagacctc 16500 aagagcttgg ttgaagaacc ctggtgagag acgttatgcg gctgtttccg gagtcagtga 16560 aaccagaagt tgctacagtg tgctgctttc tgaagcagag ggacactacg agagagagaa 16620 cagaatcagt ttggatgagg aagcacccaa gctcattgtg ttgagagcag actcccatga 16680 agagattctt ggaagattgg acaagataag ggaaaggttc ttgcagccca cgggtgctgc 16740 acccagagag tctgaattga aggcgcaagc ccgtaggatc tttcttgaac tccttggtga 16800 gacccttgcc caagatgcag cttcatctgg aagtcagaag ccccttgctc tcagcttggt 16860 ctccactcca tccaagctcc agagagaggt ggaacttgcg gcaaaaggca ttccgagatg 16920 tctcaagatg agacgtgatt ggagttcccc tgctggaagc agatatgcgc ctgagccttt 16980 ggccagtgac agagttgcct tcatgtatgg agaaggaagg agcccttact atggcatcac 17040 acaagacatt cataggattt ggcctgaact tcatgaggtc atcaatgaga aaacaaaccg 17100 tctctgggct gaaggtgaca gatgggtcat gccaagggcc agcttcaaga gtgagcttga 17160 gtcacaacag caagagtttg acagaaacat gattgaaatg ttccgtcttg gaatcctcac 17220 ctcaattgcc ttcacaaacc tggcgagaga tgttctcaac atcacgccca aagcagcctt 17280 tggactcagt ttgggagaga tttccatgat ctttgctttc tccaagaaaa acggtttgat 17340 aagcgatcag ttgacaaagg atttgagaga gtcagatgtc tggaacaaag ctctggctgt 17400 tgagttcaat gcgctcagag aagcctgggg cattccacag tctgtcccaa aagatgagtt 17460 ctggcaaggt tacatagtga ggggaacgaa acaagacata gaagcagcca tagcaccaga 17520 ctcaaagtat gtgaggttga ccatcatcaa tgatgccaac actgctctca tcagtggaaa 17580 gcctgatgca tgcaaagctg cgattgctcg tcttggtggc aacatccctg cgcttccagt 17640 cactcaaggc atgtgtgggc attgcccaga ggtgggacct tacaccaagg acatcgcaaa 17700 gatccacgcc aaccttgagt tccctgtggt tgatggtctt gatctctgga ctaccataaa 17760 ccagaagagg cttgtgccaa gagccacggg tgccaaagat gaatgggctc cttcatcctt 17820 tggggagtat gctggacagc tctatgagaa acaagccaac ttccctcaga tagttgaaac 17880 catctacaag cagaactatg atgtgtttgt tgaagttggg ccaaacaatc acagaagcac 17940 agcagtgaga accacgcttg gaccacagag aaaccacctt gctggtgcca tagacaagca 18000 gaacgaggat gcttggacga caatagtcaa gctcgtggct tcattgaagg ctcaccttgt 18060 tcctggagtg accatcagtc cgctctacca ctccaagctc gttgcagagg ctgaggcttg 18120 ttatgctgcg ctctgtaaag gtgagaaacc caagaaaaac aagtttgtga gaaagattca 18180 gttgaatggc agattcaaca gcaaggcaga ccccatctcc tcagcagacc ttgcatcctt 18240 cccacctgcg gaccctgcga ttgaagctgc catctcttca aggatcatga agcctgtggc 18300 tccgaaattc tatgcgcgtc tcaacattga tgagcaagat gagaccagag atccgatctt 18360 gaacaaggac aatgcgcctt ccagctccag ctcttccagc agttcctctt ccagcagttc 18420 atctccgtct cctgctcctt cagctccagt tcagaagaaa gctgccccag ctgcggagac 18480 aaaagctgtt gccagtgctg atgcacttcg tagtgccctg cttgatcttg actccatgct 18540 tgcgctcagc tctgcaagtg cttctggcaa ccttgttgag actgcgccat ctgatgccag 18600 tgtcattgtc cctccgtgca acattgctga tcttggcagc agagctttca tgaaaactta 18660 tggtgtttct gcgcctctgt acaccggtgc aatggccaag ggaattgcct ctgcggactt 18720 ggtgatcgca gctggaaggc aaggaatcct tgcgtcattt ggtgctggag gccttccaat 18780 gcaagttgtg cgtgagtcca ttgaaaagat tcaagctgcc ctgcccaatg gaccatatgc 18840 tgtcaacctc atccattctc cttttgacag caaccttgag aagggaaatg ttgatctctt 18900 cttggaaaag ggtgtcactt ttgttgaggc ttcagccttc atgacgttga caccgcaagt 18960 tgtcagatac agagcggctg ggttgacgag gaatgcagat gggtcagtca acataaggaa 19020 cagaatcatt gggaaagtct ccagaactga gctggctgaa atgttcatgc gtcctgcacc 19080 tgagcatctt ttgcagaagc tcattgcttc tggggagatc aatcaagaac aagctgagtt 19140 ggccagacgt gttcctgtgg ctgatgacat tgcggttgaa gctgattctg gtggacacac 19200 tgacaaccgt ccaatccatg tcattctgcc tctgattatc aacctgaggg ataggcttca 19260 cagagagtgt ggatacccag caaacctgag ggttcgtgtt ggtgctgggg gtggcattgg 19320 gtgtccccaa gctgcgctgg caactttcaa catgggtgcc tctttcattg tcactggaac 19380 agtgaaccaa gttgccaaac aatctgggac gtgtgacaat gtgaggaaac agcttgcgaa 19440 agcaacttac tcagatgttt gcatggcacc ggcagctgac atgtttgagg aaggtgtgaa 19500 acttcaagtt ttgaagaaag gaacaatgtt cccttcaaga gccaacaaac tctatgagtt 19560 gttctgcaag tatgattctt ttgagtccat gcctccagca gagcttgcga gggttgagaa 19620 aagaatcttc agtcgtgcat tggaagaggt ctgggatgag accaagaact tctacatcaa 19680 ccgtctccac aacccagaga agatacagag ggctgagagg gaccccaaac tgaagatgtc 19740 actgtgtttc agatggtatc tgtccctggc ttcaaggtgg gccaacactg gagcttctga 19800 cagagtcatg gactaccaag tttggtgtgg tcctgccatt ggttcattca atgatttcat 19860 caaaggaact taccttgatc cggcagtggc aaatgagtac ccgtgtgttg tgcagatcaa 19920 caaacagatt cttagaggag cgtgcttctt gaggcgtctt gaaatactga gaaatgcaag 19980 gctttcagat ggtgcagctg cacttgtggc gagcattgat gacacatatg tccctgctga 20040 gaagctgtga gtagttagct taatcactta ggtcaccatt ccaaacgaaa tatcctcgag 20100 agtgtgtata ccacggtgat atgagtgtgg ttgttgatgt atgttaacac tacatagtca 20160 tggtgtgtgt tccataaata atgtactaat gtaataagaa ctactccgta gacggtaata 20220 aaagagaagt tttttttttt actcttgcta ctttcctata aagtgatgat taacaacaga 20280 tacaccaaaa agaaaacaat taatctatat tcacaatgaa gcagtactag tctattgaac 20340 atgtcagatt ttctttttct agatgtctaa tcaagccttc aaggctagtg ataaaagatc 20400 atccaatggg atccaacaaa gactcaaatc tggttttgat cagatacttc gaaactattt 20460 ttgtatgcac taaattatgc aagtgttctt ttatttggtg aagactcttt agaagcaaag 20520 aacgataagc agtaataaaa aaaataaagt tcagttttaa gatttgttat tgacttagcg 20580 gccgcttaat taaactagtg ctagcctcga ggtcgaccaa cttttctata caaagttgtc 20640 tagacctagg actaggcact gagtgactca ctgacctgac tgagtgactg gcggccgcag 20700 cttatagtaa agattttttt gttaataaaa tgaaagttaa tatttatgga tttatttgaa 20760 catatttaca agtttttgtt aataaattta tttacagaag tgttaaattt gaaaatgtgt 20820 taaatgagag ttaatattta tggatataat attactttat ttaaaaataa aatgaattta 20880 tattaaaata tgatttggta aaagatatta taaagaggaa cttggatgag aaagacgacg 20940 atgggcaaaa gttgcatggc acaccgcttt tgttcagtaa catctgcact gtggcgcctt 21000 tttcacactc accatgcatg ctgccacgtc agctttcaca ctatgacacc actggccata 21060 cacgttgcca cctcagcttt ctcctcttct cactatgaca cgactggcca tgcatgcatg 21120 catgctgcca cctcagctcc cgcctcttca cccgtgtctt tctctcatgt cgcagtgctg 21180 cgtgccaacc tcttctctct ataaatagag agaagagaat gatggttaaa ctcaaaccaa 21240 aacaagaaaa catacacaaa tagcaaaacg gatccacaac caaaaatggc gcttcgtgtc 21300 aagaccaaca agaagccatg ttgggagatg accaaggagg aactgacctc tggcaaaact 21360 gaggtgttca actatgagga actcctggag tttgcagagg gtgacatagc taaggttttt 21420 ggtccggagt ttgctgtcat agacaagtac cccagaaggg tgaggctgcc tgctcgtgaa 21480 tacctgcttg tgacaagggt cacactcatg gatgccgagg tcaacaacta cagagttggt 21540 gccagaatgg tcactgagta tgatcttcca gtgaatggcg aattgtctga gggaggtgat 21600 tgtccctggg cagttctggt tgagagtgga cagtgcgatt tgatgctgat atcatacatg 21660 ggaattgatt tccagaacca aggtgacaga gtttaccgtc tgctcaacac cacgttgaca 21720 ttctatgggg ttgcccatga gggagagacc cttgagtatg acatcagagt gaccggtttt 21780 gccaaacgtt tggatggtgg catctccatg ttcttctttg agtatgactg ctatgtcaat 21840 gggaggcttc tcattgagat gagagatggc tgtgctggct tcttcacaaa tgaggagctt 21900 gatgcgggaa agggtgtggt gttcacaaga ggggatttgg ctgccagagc taagattcca 21960 aagcaagatg tttctcccta tgctgtcgct ccctgccttc acaaaacaaa actcaacgaa 22020 aaggagatgc agaccttggt tgacaaagat tgggcttccg tttttggctc aaagaatggc 22080 atgccggaga tcaactacaa actctgcgca cgtaagatgc tcatgattga tagagtcacc 22140 agcattgacc ataagggagg tgtgtacggt ttgggtcagt tggttggtga gaaaatcttg 22200 gagagggacc attggtactt cccatgccat tttgttaagg atcaagtcat ggctggatct 22260 cttgtcagcg acggttgttc acaaatgctc aagatgtaca tgatctggct cggtttgcac 22320 cttaccaccg gaccatttga cttcagaccc gtcaacggac acccgaacaa agttaggtgt 22380 aggggtcaga tatctcctca taagggaaag ttggtctacg tcatggagat caaagagatg 22440 ggattcgacg aggataacga tccgtacgcc attgctgacg tcaacatcat tgacgttgat 22500 ttcgaaaagg gtcaagattt cagccttgat agaatcagcg actacggaaa gggggatctt 22560 aacaaaaaga tcgtcgtgga cttcaagggc attgctctca agatgcagaa gagatccacg 22620 aacaagaacc cctccaaagt tcagcccgtt tttgctaacg gtgcagccac tgtgggaccg 22680 gaagcgtcca aggccagctc tggtgccagt gctagtgctt ctgctgcccc tgcgaagcct 22740 gctttctcag cagatgttct tgcgccaaag ccagttgcac ttcctgaaca tatcctcaag 22800 ggtgacgctc tcgctccgaa agaaatgtcc tggcatccca tgtcaaaact tcctggcaac 22860 ccgacgccgt tcttctcccc tagttcttac cctccgaggg caatttgctt catccctttc 22920 cctggcaacc cacttgacaa caattgcaaa gctggagaaa tgcctctgaa ctggtacaac 22980 atgtcagagt tcatgtgtgg aaaagttagc aactgcttgg gaccagagtt cgcaagattt 23040 gataagtcaa atacgagcag aagccctgct tttgatttgg ctcttgtgac tagagttgtt 23100 gaagtcacaa acatggaaca tggcaagttc ctgaatgttg attgcaatcc cagcaaaggc 23160 acaatggtgg gggagtttga ctgtcctcaa gatgcgtggt tctttgatgg ttcttgcaat 23220 gatgggcaca tgccttattc catcatcatg gaaatcggac ttcagacatc cggtgttctc 23280 accagtgtgt tgaaagcacc actgacgatg gacaaagatg acattctctt taggaacctt 23340 gatgcaagtg ctgaaatggt gaggccagat gtggatgtga gggggaaaac cataaggaat 23400 gtgaccaagt gcactggata tgcaatgttg ggaaagatgg ggattcacag attcacgttt 23460 gagttgtctg ttgatggcgt ggttttctac aaaggaagca cttcctttgg atggttcact 23520 ccagaggtgt ttgctcagca agctggactt gacaatggga aaaagacgga gccttggtgc 23580 aaaactaaca acacgagtgt gagaagagtt gaaatcgcat cagccaaagg caaagaacag 23640 ctgactgaga aacttccaga tgcaactaat gctcaagttc tcagaaggtc agaacagtgt 23700 gagtatttgg attacctcaa cattgctcct gattctgggc tgcatgggaa aggatatgcg 23760 catggacaca aagatgttaa cccacaagac tggttcttct cttgtcactt ttggtttgat 23820 cctgtgatgc ctggatcttt gggaattgag tcaatgttcc agctcattga ggcatttgcg 23880 gtggaccaga acataccggg agaatacaat gtgagcaatc cgacctttgc ccatgcccct 23940 ggaaaaacgg cgtggaagta ccgtggtcaa ttgacaccaa agaaccgtgc gatggactgt 24000 gaggtgcaca tagtttcaat cacagcctct cctgaaaatg gaggctacgt tgacatagtg 24060 gcagatggag cactttgggt tgatggtctc cgtgtgtatg aagccaaaga actcagagtt 24120 cgtgttgtca gtgcaaaacc tcaagccatt ccggatgttc aacaacagcc acccagcgca 24180 aaagcggacc ctgggaaaac tggagttgca ctcagtccga ctcagttgag agatgtcctg 24240 cttgaagtgg acaatccatt gtatcttggt gttgaaaact ccaatttggt gcagtttgaa 24300 tccaaacctg caacttcttc aaggattgtc agtatcaaac catgctccat aagtgatctt 24360 ggtgacaagt ccttcatgga aacgtacaat gtgtcagcac ctctgtacac tggagcaatg 24420 gccaagggaa ttgcgtctgc ggaccttgtc attgctgctg ggaagaggaa gatattgggt 24480 tcttttggag cgggtgggct tccaatgcac catgtgaggg cagctttgga gaagatacaa 24540 gcggctctgc ctcaaggacc atatgctgtg aacctcattc attcaccttt tgacagcaac 24600 ttggaaaagg ggaatgttga tctcttcctc gaaaagggcg ttactgtggt ggaggcttct 24660 gcattcatga ccctcactcc ccaagttgtt cgttaccgtg cagccggact ctcaaggaac 24720 gctgatggga gtgtcaacat cagaaaccgt atcattggaa aagtgtccag aacagaactc 24780 gccgaaatgt tcatcagacc ggctccggaa cacctccttg agaaactcat agcttctggt 24840 gagataacac aagaacaagc cgaacttgcc cgtcgtgttc ctgtcgcaga cgacatcgct 24900 gttgaagctg attctggtgg tcacactgac aaccgtccca tccacgtcat cttgcctttg 24960 atcatcaacc ttaggaacag actgcacaga gagtgtggtt atcctgcgca ccttagagtc 25020 agagttggag ctggaggcgg tgttggctgc ccacaagctg ctgcggctgc cctcacaatg 25080 ggagctgcct tcatagtgac tgggactgtc aatcaagttg ctaaacagtc cgggacatgt 25140 gacaacgtga gaaaacaact cagtcaagcc acctacagtg acatttgcat ggctccagca 25200 gctgatatgt ttgaggaagg tgtgaaactc caagttctca agaagggaac aatgttccca 25260 tcaagagcta acaaattgta cgaactcttt tgcaagtacg actctttcga ttcaatgcct 25320 cctgctgaac ttgaaaggat cgagaagagg atcttcaaga gggcacttca agaggtctgg 25380 gaagagacca aggactttta catcaatggt ttgaagaatc cagagaagat acagagagcg 25440 gaacatgacc ccaaactcaa gatgtctttg tgtttcagat ggtatcttgg tcttgcatca 25500 cgttgggcca acatgggtgc tcctgatcgt gtcatggact atcaagtctg gtgtggtcca 25560 gccattggag ccttcaatga cttcatcaaa ggaacatatc ttgatcctgc tgtctctaat 25620 gagtacccat gtgttgtcca gatcaacctt cagattttgc gtggtgcatg ttacctgagg 25680 cgtcttaatg ctctgagaaa tgatccgagg attgatcttg agactgaaga tgctgccttt 25740 gtctatgagc caacgaatgc gctttgagta gttagcttaa tcacttaggt cacccagtcc 25800 aagcccggcc tagcacgatg atcatgagtg ttgctattga tgtatgttaa tactatacac 25860 agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg tttgaggcta atgtaattag 25920 cactacaccg taataaatga gaggtttact ttatgtttta ataatgttct tacccttgct 25980 gctctctaaa ctgtaatggc gtacggttag ctaaactaaa gtaaaaatcg attcgcttga 26040 gtaaaattag tgaccgggaa agatcatcga ctactactgc tcagagatgt aatcggtaat 26100 taagacttca agctcaataa ttaattagga gtaaatctgg cttcgaattt ttaaattgtc 26160 aacttccaaa gaaggattgg caagactcaa tctcaattgc ccaacaaaca tatggctctt 26220 tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta attcctctgt cgatctcatg 26280 taattaccaa actcatctac ttattacttt ctttatatgc acttactgat gcggccgctt 26340 aattaatcac gagtggatga ctgtcacgac tgactcatga ctgactacta gtgctagcct 26400 cgaggtcgac acaactttgt ataataaagt tgtctagacc taggagctgt gctgggtaac 26460 tcacatgagt cactggtcac atcagcggcc gcagcttata gtaaagattt ttttgttaat 26520 aaaatgaaag ttaatattta tggatttatt tgaacatatt tacaagtttt tgttaataaa 26580 tttatttaca gaagtgttaa atttgaaaat gtgttaaatg agagttaata tttatggata 26640 taatattact ttatttaaaa ataaaatgaa tttatattaa aatatgattt ggtaaaagat 26700 attataaaga ggaacttgga tgagaaagac gacgatgggc aaaagttgca tggcacaccg 26760 cttttgttca gtaacatctg cactgtggcg cctttttcac actcaccatg catgctgcca 26820 cgtcagcttt cacactatga caccactggc catacacgtt gccacctcag ctttctcctc 26880 ttctcactat gacacgactg gccatgcatg catgcatgct gccacctcag ctcccgcctc 26940 ttcacccgtg tctttctctc atgtcgcagt gctgcgtgcc aacctcttct ctctataaat 27000 agagagaaga gaatgatggt taaactcaaa ccaaaacaag aaaacataca caaatagcaa 27060 aacggatcca caaccaaaaa tgcttcagca cacttggctt ccgaagcctc ccaatctgac 27120 cctcttgtca gatgaggttc atctctggag gattcctctt gaccagcctg agtcacaact 27180 tcaagacctt gctgccaccc tgagcagtga tgaattggcg agggcaaaca gattctactt 27240 tccagaacac agaaggcgtt tcactgctgg gagaggcatc ctcagatcca tcttgggtgg 27300 atacttggga gtggaaccgg gtcaagtcaa gtttgattat gagtcccgtg ggaaaccgat 27360 ccttggtgac agatttgctg agagtggact cctgttcaac ttgtctcaca gccagaacct 27420 tgccttgtgt gctgtcaact acacgcgtca aataggcatt gatcttgaat atctgcgtcc 27480 aacatctgac ttggagtctc ttgcaaagag gttctttctc ccaagagaat atgaactctt 27540 gaggtcactc cctgatgagc agaaacagaa gattttcttt cgttactgga cttgcaaaga 27600 ggcttatctc aaagcaacgg gagatggaat agccaaactt gaagagatcg agatagcact 27660 caccccaaca gaacctgcca agctccaaac agctcctgcg tggtctctgt tggagcttgt 27720 gccagatgac aattgtgttg cagctgtggc tgttgcgggt tttggttggc agcccaagtt 27780 ctggcattac tgagtagtta gcttaatcac ttaggtcacc cagtccaagc ccggcctagc 27840 acgatgatca tgagtgttgc tattgatgta tgttaatact atacacagtc atcgtgtgtg 27900 ttttataaaa ctgtaagctt aggatgtttg aggctaatgt aattagcact acaccgtaat 27960 aaatgagagg tttactttat gttttaataa tgttcttacc cttgctgctc tctaaactgt 28020 aatggcgtac ggttagctaa actaaagtaa aaatcgattc gcttgagtaa aattagtgac 28080 cgggaaagat catcgactac tactgctcag agatgtaatc ggtaattaag acttcaagct 28140 caataattaa ttaggagtaa atctggcttc gaatttttaa attgtcaact tccaaagaag 28200 gattggcaag actcaatctc aattgcccaa caaacatatg gctctttaca aatttggaat 28260 ctctttttga tggcaagaac gtcgtaattc ctctgtcgat ctcatgtaat taccaaactc 28320 atctacttat tactttcttt atatgcactt actgatgcgg ccgcttaatt aactactgtc 28380 actgaggccg tagacgagta cggactgatc taactagtgc tagcctcgag gtcgacaccc 28440 agctttcttg tacaaagtgg ttgcggccgc ttaattaaat ttaaattcaa ttaatgcaat 28500 cttgattttc aacaacgaag gtaatggcgt aaaagaaaaa atgtatgtta ttgtattgat 28560 ctttcatgat gttgaagcgt gccataatat gatgatgtat aattaaaata ttaactgtcg 28620 cattttattg aaatggcact gttatttcaa ccatatcttt gattctgtta catgacacga 28680 ctgcaagaag taaataatag acgccgttgt taaagaattg ctatcatatg tgcctaacta 28740 gagggaattt gagcgtcaga cctaatcaaa tattacaaaa tatctcactc tgtcgccagc 28800 aatggtgtaa tcagcgcaga caaatggcgt aaagatcgcg gaaaaacctc cccgagtggc 28860 atgatagctg cctctgtatt gctgatttag tcagccttat ttgacttaag ggtgccctcg 28920 ttagtgacaa attgctttca aggagacagc catgccccac actttgttga aaaacaaatt 28980 gcctttgggg agacggtaaa gccagttgct cttcaataag gaatgtcgag gaggcaatgt 29040 aaccgcctct ggtagtacac ttctctaatc caaaaatcaa tttgtattca agataccgca 29100 aaaaacttat ggtttaaacc ctgcaggact agtccagaag gtaattatcc aagatgtagc 29160 atcaagaatc caatgtttac gggaaaaact atggaagtat tatgtaagct cagcaagaag 29220 cagatcaata tgcggcacat atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa 29280 gatcctatac tgccagaata cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc 29340 gaagaaaaga atcttgaaga cgtaagcact gacgacaaca atgaaaagaa gaagataagg 29400 tcggtgattg tgaaagagac atagaggaca catgtaaggt ggaaaatgta agggcggaaa 29460 gtaaccttat cacaaaggaa tcttatcccc cactacttat ccttttatat ttttccgtgt 29520 catttttgcc cttgagtttt cctatataag gaaccaagtt cggcatttgt gaaaacaaga 29580 aaaaatttgg tgtaagctat tttctttgaa gtactgagga tacaacttca gagaaatttg 29640 taagtttgta ggtaccagat ctggatccca aaccatgtct ccggagagga gaccagttga 29700 gattaggcca gctacagcag ctgatatggc cgcggtttgt gatatcgtta accattacat 29760 tgagacgtct acagtgaact ttaggacaga gccacaaaca ccacaagagt ggattgatga 29820 tctagagagg ttgcaagata gatacccttg gttggttgct gaggttgagg gtgttgtggc 29880 tggtattgct tacgctgggc cctggaaggc taggaacgct tacgattgga cagttgagag 29940 tactgtttac gtgtcacata ggcatcaaag gttgggccta ggatctacat tgtacacaca 30000 tttgcttaag tctatggagg cgcaaggttt taagtctgtg gttgctgtta taggccttcc 30060 aaacgatcca tctgttaggt tgcatgaggc tttgggatac acagcccggg gtacattgcg 30120 cgcagctgga tacaagcatg gtggatggca tgatgttggt ttttggcaaa gggattttga 30180 gttgccagct cctccaaggc cagttaggcc agttacccaa atctgagtag ttagcttaat 30240 cacctagagc tcgatcggcg gcaatagctt cttagcgcca tcccgggttg atcctatctg 30300 tgttgaaata gttgcggtgg gcaaggctct ctttcagaaa gacaggcggc caaaggaacc 30360 caaggtgagg tgggctatgg ctctcagttc cttgtggaag cgcttggtct aaggtgcaga 30420 ggtgttagcg ggatgaagca aaagtgtccg attgtaacaa gatatgttga tcctacgtaa 30480 ggatattaaa gtatgtattc atcactaata taatcagtgt attccaatat gtactacgat 30540 ttccaatgtc tttattgtcg ccgtatgtaa tcggcgtcac aaaataatcc ccggtgactt 30600 tcttttaatc caggatgaaa taatatgtta ttataatttt tgcgatttgg tccgttatag 30660 gaattgaagt gtgcttgagg tcggtcgcca ccactcccat ttcataattt tacatgtatt 30720 tgaaaaataa aaatttatgg tattcaattt aaacacgtat acttgtaaag aatgatatct 30780 tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 30840 gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 30900 atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatggtg taatacatag 30960 gaattcgttt aaacgatctg cgtctaattt tcggtccaac ttgcacagga aagacgtcga 31020 ccgcggtagc tcttgcccag cagactgggc ttccagtcct ttcgctcgat cgggtccaat 31080 gttgtcctca gctgtgaacc ggaagcggac gaccaacagt ggaagaactg aaaggaacga 31140 gccgtctata ccttgatgat cggcctctgg tgaagggtat catcgcagcc aagcaagctc 31200 atgaaaggct gatgggggag gtgtataatt atgaggccca cggcgggctt attctttagg 31260 gaggatctat ctcgttgctc aagtgcatgg cgcaaagcag ttattggagt gcggattttc 31320 gttggcatat tattcgccac gagttagcag acgaagagac cttcatgaac gtggccaagg 31380 ccagagttaa gcagatgtta cgccctgctg caggcctttc tattatccaa tagttggttg 31440 atctttggaa agagcctcgg ctgaggccca tactgaaaga gatcgatgga tatcgatatg 31500 ccatgttgtt tgctagccag aaccagatca catccgatat gctattgcag cttgacgcag 31560 atatggagga taagttgatt catgggatcg ctcaggagta gctcatccat gcacgccgac 31620 aagaacagaa attccgtcga gttaacgcag ccgcttacga cggattcgaa ggtcatccat 31680 tcggaatgta ttagtttgca ccagctccgc gtcacacctg tcttcatttg aataagatgt 31740 tagcaattgt ttttagcttt gtcttgttgt ggcagggcgg caagtgcttc agacatcatt 31800 ctgttttcaa attttatgct ggagaacagc ttcttaattc ctttggaaat aatagactgc 31860 gtcttaaaat tcagatgtct ggatatagat atgattgtaa aataacctat ttaagtgtca 31920 tttagaacat aagttttatg aatgttcttc cattttcgtc atcgaacgaa taagagtaaa 31980 tacacctttt ttaacattac aaataagttc ttatacgttg tttatacacc gggaatcatt 32040 tccattattt tcgcgcaaaa gtcacggata ttcgtgaaag cgacataaac tgcgaaattt 32100 gcggggagtg tcttgagttt gcctcgaggc tagcgcatgc acatagacac acacatcatc 32160 tcattgatgc ttggtaataa ttgtcattag attgttttta tgcatagatg cactcgaaat 32220 cagccaattt tagacaagta tcaaacggat gtgacttcag tacattaaaa acgtccgcaa 32280 tgtgttatta agttgtctaa gcgtcaattt gatttacaat tgaatatatc ctgccccagc 32340 cagccaacag ctcgatttac aattgaatat atcctgccgg ccggcccacg cgtgtcgagg 32400 aattctgatc tggcccccat ttggacgtga atgtagacac gtcgaaataa agatttccga 32460 attagaataa tttgtttatt gctttcgcct ataaatacga cggatcgtaa tttgtcgttt 32520 tatcaaaatg tactttcatt ttataataac gctgcggaca tctacatttt tgaattgaaa 32580 aaaaattggt aattactctt tctttttctc catattgacc atcatactca ttgctgatcc 32640 atgtagattt cccggacatg aagccattta caattgaata tatcctgccg ccgctgccgc 32700 tttgcacccg gtggagcttg catgttggtt tctacgcaga actgagccgg ttaggcagat 32760 aatttccatt gagaactgag ccatgtgcac cttcccccca acacggtgag cgacggggca 32820 acggagtgat ccacatggga cttttaaaca tcatccgtcg gatggcgttg cgagagaagc 32880 agtcgatccg tgagatcagc cgacgcaccg ggcaggcgcg caacacgatc gcaaagtatt 32940 tgaacgcagg tacaatcgag ccgacgttca cgcggaacga ccaagcaagc ttggctgcca 33000 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg 33060 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac 33120 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt 33180 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt 33240 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc 33300 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa 33360 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat 33420 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc 33480 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc 33540 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg 33600 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc 33660 agcccagcgg cgagggcaac cagcccggtg agcgtcggaa agggtcgacg gatcttttcc 33720 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc 33780 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg 33840 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact 33900 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct 33960 accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag 34020 ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag 34080 gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa 34140 atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg 34200 ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc 34260 ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc 34320 aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa 34380 cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga 34440 cttcgcggag ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg 34500 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc 34560 accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc 34620 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga 34680 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc 34740 ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg 34800 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc 34860 gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg 34920 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga 34980 ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc 35040 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg 35100 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt 35160 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga 35220 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga 35280 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg 35340 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac 35400 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga 35460 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt 35520 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc 35580 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac 35640 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg 35700 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga 35760 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc 35820 cagcgcttta ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca 35880 gtatcgctcg ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg 35940 acaattgtga ttaaggctca gattcgacgg cttggagcgg ccgacgtgca ggatttccgc 36000 gagatccgat tgtcggccct gaagaaagct ccagagatgt tcgggtccgt ttacgagcac 36060 gaggagaaaa agcccatgga ggcgttcgct gaacggttgc gagatgccgt ggcattcggc 36120 gcctacatcg acggcgagat cattgggctg tcggtcttca aacaggagga cggccccaag 36180 gacgctcaca aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg aggccgaggg 36240 gtcgccggta tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga 36300 cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg 36360 ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg 36420 gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg 36480 atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg 36540 ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt 36600 tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc 36660 tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt 36720 gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg 36780 atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca 36840 gttgtttcct tactgggctt tctcagcccc cgagcgctta gtgggaattt gtacccctta 36900 tcgaaccggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc 36960 gcggcttaat tcaggagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc 37020 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac 37080 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg 37140 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg 37200 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg 37260 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat 37320 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg 37380 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg 37440 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc 37500 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc 37560 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg 37620 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctagg caggcttatc 37680 ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg 37740 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac 37800 gccgcttcgc ggcgcggctt aactcaagcg ttagagagct ggggaagact atgcgcgatc 37860 tgttgaaggt ggttctaagc ctcgtcttgc gatggcattt cgatccattc ccattccgcg 37920 ctcaagatgg cttcccctcg gcagttcatc agggctaaat caatctagcc gacttgtccg 37980 gtgaaatggg ctgcactcca acagaaacaa tcaaacaaac atacacagcg acttattcac 38040 acgagctcaa attacaacgg tatatatcct gccagtcagc atcatcacac caaaagttag 38100 gcccgaatag tttgaaatta gaaagctcgc aattgaggtc tacaggccaa attcgctctt 38160 agccgtacaa tattactcac cggatcctaa ccggtttaat taaggcgcgc catgcccggg 38220 caagcggccg cacaagttt 38239 <210> 62 <211> 34850 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB109509 <400> 62 gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat ctcagcatgt 60 gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat gaaagttaat 120 atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat ttacagaagt 180 gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata ttactttatt 240 taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat aaagaggaac 300 ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt gttcagtaac 360 atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca gctttcacac 420 tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc actatgacac 480 gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac ccgtgtcttt 540 ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga gaagagaatg 600 atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg atccacaacc 660 aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga ggattgcgat 720 cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat gggagaccat 780 aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg tgactgcgta 840 ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag gtttcattcc 900 tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg aagattctga 960 tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg ctgggattga 1020 tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag gtggacagaa 1080 gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag tcttgagaaa 1140 gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag cgaacttccc 1200 agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg gcagatgcac 1260 caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg caagctcctt 1320 gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca tgatggtcac 1380 tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa agacacctgt 1440 gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga tgctcattgg 1500 tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg atggtgatga 1560 gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag ctggcatcta 1620 cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc gtgcctgcgt 1680 tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag ttggagatag 1740 gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag ggaacacaga 1800 gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg ttgctggtct 1860 tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg gaacgatcaa 1920 tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat ccctctacat 1980 caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg ctggaatatc 2040 cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg aaccagagca 2100 cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg ctgcaactcc 2160 tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg cgatcaagga 2220 gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag aacagttcaa 2280 gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca aagatgcgga 2340 ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg tgaccaaaga 2400 agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg ccaccaatgg 2460 tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt tcagtgaagt 2520 tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg cacagtccaa 2580 ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc caaggaaacc 2640 gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat acagccagcc 2700 gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg gattcactcc 2760 agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag ctggttgtgt 2820 tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg gaggcaaaga 2880 tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg cagagaacat 2940 caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc agactgtcat 3000 tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg aagggttcag 3060 agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa atgccagctc 3120 agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag aaacaaagct 3180 cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc tcactcaaca 3240 catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag ctggtgcgag 3300 aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg aaaccctcaa 3360 agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg actctgacat 3420 acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc aagggtttga 3480 caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga gaaccacact 3540 cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg atgctgccat 3600 gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca aggctcctga 3660 acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg agcttcaaga 3720 cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact ccaaacttgc 3780 tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag ttgacactgc 3840 tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg atggttatgg 3900 aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc cggcgcctgt 3960 taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg tgtccaacga 4020 gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga ctggatacga 4080 aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg acagcatcaa 4140 gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga aagatgttga 4200 tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg cagaaatcgc 4260 tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag cggctccagc 4320 tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg ttgtgatgga 4380 agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca tggagcttga 4440 gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag tccaagccat 4500 gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg tcggtgaggt 4560 tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg ccgctgccgc 4620 tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg tctcaaacga 4680 actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa ccggctatga 4740 gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag acagcatcaa 4800 acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga aggatgttga 4860 tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg ctgaaattgc 4920 tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg cggcacctgc 4980 tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg agacggttgt 5040 gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat ctgatatgga 5100 attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga gtgaagtcca 5160 agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa ggacagtggg 5220 tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg cgccagctgc 5280 cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac cggccgcgcc 5340 cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga tggaggtgct 5400 cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc ttgaaaccga 5460 gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag ctatgttgaa 5520 cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg aggtcgtgga 5580 cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag ccgcacctgc 5640 acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca atgagttgct 5700 ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt acgaaacgga 5760 catgattgag tcagacatgg aattggagac cgaacttgga atagattcca tcaagagagt 5820 ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg ttgatgcact 5880 cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga ttgctggagg 5940 ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac cagctgtcag 6000 caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg caaaaaccgg 6060 ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg gcattgacag 6120 catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg aggctaagga 6180 tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga tgaaggcgga 6240 gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg cagccgcggc 6300 accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa aggctgagac 6360 agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga ttgaatctga 6420 catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa tcctctctga 6480 ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca gaacaaggac 6540 ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg ctcctgcgcc 6600 agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg aactccttga 6660 aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg agactgacat 6720 gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca agcgtgtgga 6780 aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg atgctttgtc 6840 aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag ctggtggatc 6900 agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag ctgtgaagat 6960 tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa aggtggttga 7020 catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag ttcttgtcgt 7080 tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct gtgcagttgt 7140 cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca ttcgtcatgt 7200 tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg ctgagcaaag 7260 atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac cagctgagat 7320 actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca ctgctgtggc 7380 tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg gattcacctc 7440 tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg gactctgcaa 7500 gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg acattgctca 7560 agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt gtgctgacat 7620 cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca gagctgccaa 7680 gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc tggtttctgg 7740 aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga ttgctggagg 7800 caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat ggtgtgctgg 7860 catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga gggccttcag 7920 tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg gatctgttct 7980 tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg gcaaagcgat 8040 ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta gggatgcaga 8100 gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc tccgtgatcg 8160 tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca aagtcactgg 8220 gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg ttctcttcag 8280 ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg caaacgaagc 8340 tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca tctgctttgg 8400 tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag aaatgggagt 8460 tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt tgggatcaag 8520 tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg gaagtgacac 8580 gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg atcatgtcat 8640 acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg agacctgcct 8700 tggactcttc cctggctact ctctctgggc catagatgac gcacagctct tcaaaggtgt 8760 cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg ctccgagtgg 8820 gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg ttccagccta 8880 cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa ccatgcagcc 8940 tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca agactctctt 9000 tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga gccagcttgt 9060 tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg caactgacac 9120 tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg tctgggtgag 9180 gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc aacacagacc 9240 ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg gtggacatag 9300 ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca ttgatgttca 9360 agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa tcacttaggt 9420 cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga tgtatgttaa 9480 tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg tttgaggcta 9540 atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta ataatgttct 9600 tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa gtaaaaatcg 9660 attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc tcagagatgt 9720 aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg cttcgaattt 9780 ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc ccaacaaaca 9840 tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta attcctctgt 9900 cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc acttactgat 9960 gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg ctgatcacta 10020 gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctagacc taggctcagc 10080 aactcatcca gtcaagttcg agtcagtgag ttgggcggcc gcagcttata gtaaagattt 10140 ttttgttaat aaaatgaaag ttaatattta tggatttatt tgaacatatt tacaagtttt 10200 tgttaataaa tttatttaca gaagtgttaa atttgaaaat gtgttaaatg agagttaata 10260 tttatggata taatattact ttatttaaaa ataaaatgaa tttatattaa aatatgattt 10320 ggtaaaagat attataaaga ggaacttgga tgagaaagac gacgatgggc aaaagttgca 10380 tggcacaccg cttttgttca gtaacatctg cactgtggcg cctttttcac actcaccatg 10440 catgctgcca cgtcagcttt cacactatga caccactggc catacacgtt gccacctcag 10500 ctttctcctc ttctcactat gacacgactg gccatgcatg catgcatgct gccacctcag 10560 ctcccgcctc ttcacccgtg tctttctctc atgtcgcagt gctgcgtgcc aacctcttct 10620 ctctataaat agagagaaga gaatgatggt taaactcaaa ccaaaacaag aaaacataca 10680 caaatagcaa aacggatcca caaccaaaaa tggctgcaag gaacgtgagt gcggctcatg 10740 agatgcatga cgagaagagg atagctgttg tgggcatggc tgttcagtat gctggatgca 10800 aaacgaaaga cgagttctgg gaagttctca tgaatggaaa agttgagagc aaagtgatca 10860 gcgacaaacg tcttggttca aattaccgtg ctgaacacta caaagctgaa aggagcaagt 10920 atgctgacac cttctgcaat gagacatatg gaactcttga tgagaacgaa atagacaatg 10980 agcatgaact ccttttgaac cttgccaaac aagccttggc tgaaacatct gtcaaagatt 11040 caacaagatg tggcatagtt tctggatgcc tcagcttccc gatggacaac ctccaaggtg 11100 aactcttgaa tgtgtaccag aaccatgttg agaagaaact tggtgccaga gttttcaaag 11160 atgcctctca ctggtcagaa agagaacagt ccaacaaacc agaggctggt gacagaagaa 11220 tcttcatgga ccctgcttcc tttgttgctg aggaactcaa tcttggcgct ctccactact 11280 ctgttgatgc tgcctgtgcc actgcgctct atgtgctcag actggcgcaa gatcacttgg 11340 tctctggtgc agctgatgtg atgctttgtg gtgcaacatg cctgccggag ccgtttttca 11400 tactctctgg gttctcaacc ttccaagcca tgcctgttgg aacgggtcag aatgtgtcca 11460 tgccgctgca caaagattct caaggattga cccctggtga aggaggttcc atcatggtcc 11520 tcaaacgttt ggatgacgca atcagagatg gagatcacat ctatggaaca cttttgggag 11580 ccaatgtcag caacagtggc actggtctgc cacttaaacc ccttttgccg tcagagaaaa 11640 agtgcctcat ggacacctac acacgtatca atgtgcatcc ccacaagatt cagtatgttg 11700 aatgccatgc aactgggact ccacaaggtg atcgtgtgga aattgatgct gtgaaggctt 11760 gctttgaagg caaagtccca cgttttggaa ccacaaaagg gaactttgga cacacattgg 11820 tggcagctgg gtttgctggc atgtgcaaag tgcttctctc catgaagcac gggatcattc 11880 cacccactcc gggaatagat gacgaaacga agatggaccc tctggttgtc tctggtgagg 11940 ccattccctg gccagaaacg aatggagaac cgaagcgtgc tggtctctca gcgtttggat 12000 ttggtgggac caatgctcat gctgtctttg aggaacatga cccaagcaat gctgcatgca 12060 ctggccatga ctccatttct gctctctctg cacgttgtgg tggtgagtca aacatgagaa 12120 tagcaatcac tgggatggat gccacttttg gtgctttgaa aggattggat gcttttgaaa 12180 gggccatcta cactggtgct catggtgcta taccactccc agaaaagaga tggagattcc 12240 tggggaaaga caaggacttc cttgatcttt gtggagtcaa agcaacaccg catggctgtt 12300 acattgaaga tgttgaagtt gatttccaga ggctgaggac tccaatgact cctgaagata 12360 tgctcttgcc tcaacagctt ctggctgtca ccacaattga tagggccata ttggactctg 12420 gcatgaagaa aggtggaaat gtggctgtct ttgtggggtt ggggaccgat cttgaactct 12480 accgtcaccg tgcaagggtt gctctcaagg agagagtgag gcctgaagcc agcaagaaac 12540 tcaatgacat gatgcagtac atcaatgatt gtgggacaag cacttcttac accagctaca 12600 taggcaactt ggttgcaaca agggtcagtt cacaatgggg cttcacggga ccttccttca 12660 caatcacaga aggcaacaat tctgtgtaca gatgtgctga acttggcaaa tacctcttgg 12720 aaacgggaga ggttgatggg gttgtggttg cgggtgttga tctctgtggg agtgctgaga 12780 acctttacgt caagtcaagg cgtttcaagg tgtctacatc tgacaccccg agagccagct 12840 ttgatgcagc tgctgatggt tactttgttg gagagggctg tggtgcattt gtgctcaagc 12900 gtgagacaag ctgcaccaag gatgacagaa tctatgcttg catggatgcc atagttcctg 12960 gaaatgttcc atctgcatgt ttgagagagg cattggatca agccagagtc aagcctggag 13020 acatcgagat gttggagctt tctgctgatt cagcgaggca tttgaaggac ccgtctgttc 13080 tcccaaagga actcactgct gaggaagaga ttggcggact tcagacgatt cttcgtgacg 13140 atgacaagct cccaaggaat gttgcaactg gaagtgtcaa ggccactgtt ggtgacactg 13200 gttatgcttc tggtgctgcc agtttgatta aggctgcgct ttgcatctac aaccgttacc 13260 tgcccagcaa tggagatgac tgggatgaac ctgcacctga agcaccctgg gacagcacac 13320 tctttgcgtg ccagacctca agagcttggt tgaagaaccc tggtgagaga cgttatgcgg 13380 ctgtttccgg agtcagtgaa accagaagtt gctacagtgt gctgctttct gaagcagagg 13440 gacactacga gagagagaac agaatcagtt tggatgagga agcacccaag ctcattgtgt 13500 tgagagcaga ctcccatgaa gagattcttg gaagattgga caagataagg gaaaggttct 13560 tgcagcccac gggtgctgca cccagagagt ctgaattgaa ggcgcaagcc cgtaggatct 13620 ttcttgaact ccttggtgag acccttgccc aagatgcagc ttcatctgga agtcagaagc 13680 cccttgctct cagcttggtc tccactccat ccaagctcca gagagaggtg gaacttgcgg 13740 caaaaggcat tccgagatgt ctcaagatga gacgtgattg gagttcccct gctggaagca 13800 gatatgcgcc tgagcctttg gccagtgaca gagttgcctt catgtatgga gaaggaagga 13860 gcccttacta tggcatcaca caagacattc ataggatttg gcctgaactt catgaggtca 13920 tcaatgagaa aacaaaccgt ctctgggctg aaggtgacag atgggtcatg ccaagggcca 13980 gcttcaagag tgagcttgag tcacaacagc aagagtttga cagaaacatg attgaaatgt 14040 tccgtcttgg aatcctcacc tcaattgcct tcacaaacct ggcgagagat gttctcaaca 14100 tcacgcccaa agcagccttt ggactcagtt tgggagagat ttccatgatc tttgctttct 14160 ccaagaaaaa cggtttgata agcgatcagt tgacaaagga tttgagagag tcagatgtct 14220 ggaacaaagc tctggctgtt gagttcaatg cgctcagaga agcctggggc attccacagt 14280 ctgtcccaaa agatgagttc tggcaaggtt acatagtgag gggaacgaaa caagacatag 14340 aagcagccat agcaccagac tcaaagtatg tgaggttgac catcatcaat gatgccaaca 14400 ctgctctcat cagtggaaag cctgatgcat gcaaagctgc gattgctcgt cttggtggca 14460 acatccctgc gcttccagtc actcaaggca tgtgtgggca ttgcccagag gtgggacctt 14520 acaccaagga catcgcaaag atccacgcca accttgagtt ccctgtggtt gatggtcttg 14580 atctctggac taccataaac cagaagaggc ttgtgccaag agccacgggt gccaaagatg 14640 aatgggctcc ttcatccttt ggggagtatg ctggacagct ctatgagaaa caagccaact 14700 tccctcagat agttgaaacc atctacaagc agaactatga tgtgtttgtt gaagttgggc 14760 caaacaatca cagaagcaca gcagtgagaa ccacgcttgg accacagaga aaccaccttg 14820 ctggtgccat agacaagcag aacgaggatg cttggacgac aatagtcaag ctcgtggctt 14880 cattgaaggc tcaccttgtt cctggagtga ccatcagtcc gctctaccac tccaagctcg 14940 ttgcagaggc tgaggcttgt tatgctgcgc tctgtaaagg tgagaaaccc aagaaaaaca 15000 agtttgtgag aaagattcag ttgaatggca gattcaacag caaggcagac cccatctcct 15060 cagcagacct tgcatccttc ccacctgcgg accctgcgat tgaagctgcc atctcttcaa 15120 ggatcatgaa gcctgtggct ccgaaattct atgcgcgtct caacattgat gagcaagatg 15180 agaccagaga tccgatcttg aacaaggaca atgcgccttc cagctccagc tcttccagca 15240 gttcctcttc cagcagttca tctccgtctc ctgctccttc agctccagtt cagaagaaag 15300 ctgccccagc tgcggagaca aaagctgttg ccagtgctga tgcacttcgt agtgccctgc 15360 ttgatcttga ctccatgctt gcgctcagct ctgcaagtgc ttctggcaac cttgttgaga 15420 ctgcgccatc tgatgccagt gtcattgtcc ctccgtgcaa cattgctgat cttggcagca 15480 gagctttcat gaaaacttat ggtgtttctg cgcctctgta caccggtgca atggccaagg 15540 gaattgcctc tgcggacttg gtgatcgcag ctggaaggca aggaatcctt gcgtcatttg 15600 gtgctggagg ccttccaatg caagttgtgc gtgagtccat tgaaaagatt caagctgccc 15660 tgcccaatgg accatatgct gtcaacctca tccattctcc ttttgacagc aaccttgaga 15720 agggaaatgt tgatctcttc ttggaaaagg gtgtcacttt tgttgaggct tcagccttca 15780 tgacgttgac accgcaagtt gtcagataca gagcggctgg gttgacgagg aatgcagatg 15840 ggtcagtcaa cataaggaac agaatcattg ggaaagtctc cagaactgag ctggctgaaa 15900 tgttcatgcg tcctgcacct gagcatcttt tgcagaagct cattgcttct ggggagatca 15960 atcaagaaca agctgagttg gccagacgtg ttcctgtggc tgatgacatt gcggttgaag 16020 ctgattctgg tggacacact gacaaccgtc caatccatgt cattctgcct ctgattatca 16080 acctgaggga taggcttcac agagagtgtg gatacccagc aaacctgagg gttcgtgttg 16140 gtgctggggg tggcattggg tgtccccaag ctgcgctggc aactttcaac atgggtgcct 16200 ctttcattgt cactggaaca gtgaaccaag ttgccaaaca atctgggacg tgtgacaatg 16260 tgaggaaaca gcttgcgaaa gcaacttact cagatgtttg catggcaccg gcagctgaca 16320 tgtttgagga aggtgtgaaa cttcaagttt tgaagaaagg aacaatgttc ccttcaagag 16380 ccaacaaact ctatgagttg ttctgcaagt atgattcttt tgagtccatg cctccagcag 16440 agcttgcgag ggttgagaaa agaatcttca gtcgtgcatt ggaagaggtc tgggatgaga 16500 ccaagaactt ctacatcaac cgtctccaca acccagagaa gatacagagg gctgagaggg 16560 accccaaact gaagatgtca ctgtgtttca gatggtatct gtccctggct tcaaggtggg 16620 ccaacactgg agcttctgac agagtcatgg actaccaagt ttggtgtggt cctgccattg 16680 gttcattcaa tgatttcatc aaaggaactt accttgatcc ggcagtggca aatgagtacc 16740 cgtgtgttgt gcagatcaac aaacagattc ttagaggagc gtgcttcttg aggcgtcttg 16800 aaatactgag aaatgcaagg ctttcagatg gtgcagctgc acttgtggcg agcattgatg 16860 acacatatgt ccctgctgag aagctgtgag tagttagctt aatcacttag gtcacccagt 16920 ccaagcccgg cctagcacga tgatcatgag tgttgctatt gatgtatgtt aatactatac 16980 acagtcatcg tgtgtgtttt ataaaactgt aagcttagga tgtttgaggc taatgtaatt 17040 agcactacac cgtaataaat gagaggttta ctttatgttt taataatgtt cttacccttg 17100 ctgctctcta aactgtaatg gcgtacggtt agctaaacta aagtaaaaat cgattcgctt 17160 gagtaaaatt agtgaccggg aaagatcatc gactactact gctcagagat gtaatcggta 17220 attaagactt caagctcaat aattaattag gagtaaatct ggcttcgaat ttttaaattg 17280 tcaacttcca aagaaggatt ggcaagactc aatctcaatt gcccaacaaa catatggctc 17340 tttacaaatt tggaatctct ttttgatggc aagaacgtcg taattcctct gtcgatctca 17400 tgtaattacc aaactcatct acttattact ttctttatat gcacttactg atgcggccgc 17460 ttaattaatc tgacctgacc actagacctg agtgagtaca gatgtgctac tagtgctagc 17520 ctcgaggtcg accaactttt ctatacaaag ttgtctagac ctaggactag gcactgagtg 17580 actcactgac ctgactgagt gactggcggc cgcagcttat agtaaagatt tttttgttaa 17640 taaaatgaaa gttaatattt atggatttat ttgaacatat ttacaagttt ttgttaataa 17700 atttatttac agaagtgtta aatttgaaaa tgtgttaaat gagagttaat atttatggat 17760 ataatattac tttatttaaa aataaaatga atttatatta aaatatgatt tggtaaaaga 17820 tattataaag aggaacttgg atgagaaaga cgacgatggg caaaagttgc atggcacacc 17880 gcttttgttc agtaacatct gcactgtggc gcctttttca cactcaccat gcatgctgcc 17940 acgtcagctt tcacactatg acaccactgg ccatacacgt tgccacctca gctttctcct 18000 cttctcacta tgacacgact ggccatgcat gcatgcatgc tgccacctca gctcccgcct 18060 cttcacccgt gtctttctct catgtcgcag tgctgcgtgc caacctcttc tctctataaa 18120 tagagagaag agaatgatgg ttaaactcaa accaaaacaa gaaaacatac acaaatagca 18180 aaacggatcc acaaccaaaa atggcgcttc gtgtcaagac caacaagaag ccatgttggg 18240 agatgaccaa ggaggaactg acctctggca aaactgaggt gttcaactat gaggaactcc 18300 tggagtttgc agagggtgac atagctaagg tttttggtcc ggagtttgct gtcatagaca 18360 agtaccccag aagggtgagg ctgcctgctc gtgaatacct gcttgtgaca agggtcacac 18420 tcatggatgc cgaggtcaac aactacagag ttggtgccag aatggtcact gagtatgatc 18480 ttccagtgaa tggcgaattg tctgagggag gtgattgtcc ctgggcagtt ctggttgaga 18540 gtggacagtg cgatttgatg ctgatatcat acatgggaat tgatttccag aaccaaggtg 18600 acagagttta ccgtctgctc aacaccacgt tgacattcta tggggttgcc catgagggag 18660 agacccttga gtatgacatc agagtgaccg gttttgccaa acgtttggat ggtggcatct 18720 ccatgttctt ctttgagtat gactgctatg tcaatgggag gcttctcatt gagatgagag 18780 atggctgtgc tggcttcttc acaaatgagg agcttgatgc gggaaagggt gtggtgttca 18840 caagagggga tttggctgcc agagctaaga ttccaaagca agatgtttct ccctatgctg 18900 tcgctccctg ccttcacaaa acaaaactca acgaaaagga gatgcagacc ttggttgaca 18960 aagattgggc ttccgttttt ggctcaaaga atggcatgcc ggagatcaac tacaaactct 19020 gcgcacgtaa gatgctcatg attgatagag tcaccagcat tgaccataag ggaggtgtgt 19080 acggtttggg tcagttggtt ggtgagaaaa tcttggagag ggaccattgg tacttcccat 19140 gccattttgt taaggatcaa gtcatggctg gatctcttgt cagcgacggt tgttcacaaa 19200 tgctcaagat gtacatgatc tggctcggtt tgcaccttac caccggacca tttgacttca 19260 gacccgtcaa cggacacccg aacaaagtta ggtgtagggg tcagatatct cctcataagg 19320 gaaagttggt ctacgtcatg gagatcaaag agatgggatt cgacgaggat aacgatccgt 19380 acgccattgc tgacgtcaac atcattgacg ttgatttcga aaagggtcaa gatttcagcc 19440 ttgatagaat cagcgactac ggaaaggggg atcttaacaa aaagatcgtc gtggacttca 19500 agggcattgc tctcaagatg cagaagagat ccacgaacaa gaacccctcc aaagttcagc 19560 ccgtttttgc taacggtgca gccactgtgg gaccggaagc gtccaaggcc agctctggtg 19620 ccagtgctag tgcttctgct gcccctgcga agcctgcttt ctcagcagat gttcttgcgc 19680 caaagccagt tgcacttcct gaacatatcc tcaagggtga cgctctcgct ccgaaagaaa 19740 tgtcctggca tcccatgtca aaacttcctg gcaacccgac gccgttcttc tcccctagtt 19800 cttaccctcc gagggcaatt tgcttcatcc ctttccctgg caacccactt gacaacaatt 19860 gcaaagctgg agaaatgcct ctgaactggt acaacatgtc agagttcatg tgtggaaaag 19920 ttagcaactg cttgggacca gagttcgcaa gatttgataa gtcaaatacg agcagaagcc 19980 ctgcttttga tttggctctt gtgactagag ttgttgaagt cacaaacatg gaacatggca 20040 agttcctgaa tgttgattgc aatcccagca aaggcacaat ggtgggggag tttgactgtc 20100 ctcaagatgc gtggttcttt gatggttctt gcaatgatgg gcacatgcct tattccatca 20160 tcatggaaat cggacttcag acatccggtg ttctcaccag tgtgttgaaa gcaccactga 20220 cgatggacaa agatgacatt ctctttagga accttgatgc aagtgctgaa atggtgaggc 20280 cagatgtgga tgtgaggggg aaaaccataa ggaatgtgac caagtgcact ggatatgcaa 20340 tgttgggaaa gatggggatt cacagattca cgtttgagtt gtctgttgat ggcgtggttt 20400 tctacaaagg aagcacttcc tttggatggt tcactccaga ggtgtttgct cagcaagctg 20460 gacttgacaa tgggaaaaag acggagcctt ggtgcaaaac taacaacacg agtgtgagaa 20520 gagttgaaat cgcatcagcc aaaggcaaag aacagctgac tgagaaactt ccagatgcaa 20580 ctaatgctca agttctcaga aggtcagaac agtgtgagta tttggattac ctcaacattg 20640 ctcctgattc tgggctgcat gggaaaggat atgcgcatgg acacaaagat gttaacccac 20700 aagactggtt cttctcttgt cacttttggt ttgatcctgt gatgcctgga tctttgggaa 20760 ttgagtcaat gttccagctc attgaggcat ttgcggtgga ccagaacata ccgggagaat 20820 acaatgtgag caatccgacc tttgcccatg cccctggaaa aacggcgtgg aagtaccgtg 20880 gtcaattgac accaaagaac cgtgcgatgg actgtgaggt gcacatagtt tcaatcacag 20940 cctctcctga aaatggaggc tacgttgaca tagtggcaga tggagcactt tgggttgatg 21000 gtctccgtgt gtatgaagcc aaagaactca gagttcgtgt tgtcagtgca aaacctcaag 21060 ccattccgga tgttcaacaa cagccaccca gcgcaaaagc ggaccctggg aaaactggag 21120 ttgcactcag tccgactcag ttgagagatg tcctgcttga agtggacaat ccattgtatc 21180 ttggtgttga aaactccaat ttggtgcagt ttgaatccaa acctgcaact tcttcaagga 21240 ttgtcagtat caaaccatgc tccataagtg atcttggtga caagtccttc atggaaacgt 21300 acaatgtgtc agcacctctg tacactggag caatggccaa gggaattgcg tctgcggacc 21360 ttgtcattgc tgctgggaag aggaagatat tgggttcttt tggagcgggt gggcttccaa 21420 tgcaccatgt gagggcagct ttggagaaga tacaagcggc tctgcctcaa ggaccatatg 21480 ctgtgaacct cattcattca ccttttgaca gcaacttgga aaaggggaat gttgatctct 21540 tcctcgaaaa gggcgttact gtggtggagg cttctgcatt catgaccctc actccccaag 21600 ttgttcgtta ccgtgcagcc ggactctcaa ggaacgctga tgggagtgtc aacatcagaa 21660 accgtatcat tggaaaagtg tccagaacag aactcgccga aatgttcatc agaccggctc 21720 cggaacacct ccttgagaaa ctcatagctt ctggtgagat aacacaagaa caagccgaac 21780 ttgcccgtcg tgttcctgtc gcagacgaca tcgctgttga agctgattct ggtggtcaca 21840 ctgacaaccg tcccatccac gtcatcttgc ctttgatcat caaccttagg aacagactgc 21900 acagagagtg tggttatcct gcgcacctta gagtcagagt tggagctgga ggcggtgttg 21960 gctgcccaca agctgctgcg gctgccctca caatgggagc tgccttcata gtgactggga 22020 ctgtcaatca agttgctaaa cagtccggga catgtgacaa cgtgagaaaa caactcagtc 22080 aagccaccta cagtgacatt tgcatggctc cagcagctga tatgtttgag gaaggtgtga 22140 aactccaagt tctcaagaag ggaacaatgt tcccatcaag agctaacaaa ttgtacgaac 22200 tcttttgcaa gtacgactct ttcgattcaa tgcctcctgc tgaacttgaa aggatcgaga 22260 agaggatctt caagagggca cttcaagagg tctgggaaga gaccaaggac ttttacatca 22320 atggtttgaa gaatccagag aagatacaga gagcggaaca tgaccccaaa ctcaagatgt 22380 ctttgtgttt cagatggtat cttggtcttg catcacgttg ggccaacatg ggtgctcctg 22440 atcgtgtcat ggactatcaa gtctggtgtg gtccagccat tggagccttc aatgacttca 22500 tcaaaggaac atatcttgat cctgctgtct ctaatgagta cccatgtgtt gtccagatca 22560 accttcagat tttgcgtggt gcatgttacc tgaggcgtct taatgctctg agaaatgatc 22620 cgaggattga tcttgagact gaagatgctg cctttgtcta tgagccaacg aatgcgcttt 22680 gagtagttag cttaatcact taggtcaccc agtccaagcc cggcctagca cgatgatcat 22740 gagtgttgct attgatgtat gttaatacta tacacagtca tcgtgtgtgt tttataaaac 22800 tgtaagctta ggatgtttga ggctaatgta attagcacta caccgtaata aatgagaggt 22860 ttactttatg ttttaataat gttcttaccc ttgctgctct ctaaactgta atggcgtacg 22920 gttagctaaa ctaaagtaaa aatcgattcg cttgagtaaa attagtgacc gggaaagatc 22980 atcgactact actgctcaga gatgtaatcg gtaattaaga cttcaagctc aataattaat 23040 taggagtaaa tctggcttcg aatttttaaa ttgtcaactt ccaaagaagg attggcaaga 23100 ctcaatctca attgcccaac aaacatatgg ctctttacaa atttggaatc tctttttgat 23160 ggcaagaacg tcgtaattcc tctgtcgatc tcatgtaatt accaaactca tctacttatt 23220 actttcttta tatgcactta ctgatgcggc cgcttaatta atcacgagtg gatgactgtc 23280 acgactgact catgactgac tactagtgct agcctcgagg tcgacacaac tttgtataat 23340 aaagttgtct agacctagga gctgtgctgg gtaactcaca tgagtcactg gtcacatcag 23400 cggccgcaga tctgattggt aagatatggg tactgtttgg tttatatgtt ttgactattc 23460 agtcactatg gcccccataa attttaattc ggctggtatg tctcggttaa gaccggtttg 23520 acatggttca tttcagttca attatgttaa tctggcacgt gatatgttta ccttcacacg 23580 aacattagta atgatgggct aatttaagac ttaacaggcc tagagaggcc catcttatta 23640 cgtaacggcg tcgtttagag tgcaccaagc ttataaatga aaacgagcta cctcgggaca 23700 tcacgctctt tgtacactcc gccatctctc tctctcgttg gagcagatct ctctcgtgaa 23760 tatcgacaat gcttcagcac acttggcttc cgaagcctcc caatctgacc ctcttgtcag 23820 atgaggttca tctctggagg attcctcttg accagcctga gtcacaactt caagaccttg 23880 ctgccaccct gagcagtgat gaattggcga gggcaaacag attctacttt ccagaacaca 23940 gaaggcgttt cactgctggg agaggcatcc tcagatccat cttgggtgga tacttgggag 24000 tggaaccggg tcaagtcaag tttgattatg agtcccgtgg gaaaccgatc cttggtgaca 24060 gatttgctga gagtggactc ctgttcaact tgtctcacag ccagaacctt gccttgtgtg 24120 ctgtcaacta cacgcgtcaa ataggcattg atcttgaata tctgcgtcca acatctgact 24180 tggagtctct tgcaaagagg ttctttctcc caagagaata tgaactcttg aggtcactcc 24240 ctgatgagca gaaacagaag attttctttc gttactggac ttgcaaagag gcttatctca 24300 aagcaacggg agatggaata gccaaacttg aagagatcga gatagcactc accccaacag 24360 aacctgccaa gctccaaaca gctcctgcgt ggtctctgtt ggagcttgtg ccagatgaca 24420 attgtgttgc agctgtggct gttgcgggtt ttggttggca gcccaagttc tggcattact 24480 gagtagttag cttaatcact taggtcaccc tatcaaaatc tatttagaaa tacacaatat 24540 tttgttgcag gcttgctgga gaatcgatct gctatcataa aaattacaaa aaaattttat 24600 ttgcctcaat tattttagga ttggtattaa ggacgcttaa attatttgtc gggtcactac 24660 gcatcattgt gattgagaag atcagcgata cgaaatattc gtagtactat cgataattta 24720 tttgaaaatt cataagaaaa gcaaacgtta catgaattga tgaaacaata caaagacaga 24780 taaagccacg cacatttagg atattggccg agattactga atattgagta agatcacgga 24840 atttctgaca ggagcatgtc ttcaattcag cccaaatggc agttgaaata ctcaaaccgc 24900 cccatatgca ggagcggatc attcattgtt tgtttggttg cctttgccaa catgggagtc 24960 caaggttgcg gccgcttaat taactactgt cactgaggcc gtagacgagt acggactgat 25020 ctaactagtg ctagcctcga ggtcgacacc cagctttctt gtacaaagtg gttgcggccg 25080 cttaattaaa tttaaattca attaatgcaa tcttgatttt caacaacgaa ggtaatggcg 25140 taaaagaaaa aatgtatgtt attgtattga tctttcatga tgttgaagcg tgccataata 25200 tgatgatgta taattaaaat attaactgtc gcattttatt gaaatggcac tgttatttca 25260 accatatctt tgattctgtt acatgacacg actgcaagaa gtaaataata gacgccgttg 25320 ttaaagaatt gctatcatat gtgcctaact agagggaatt tgagcgtcag acctaatcaa 25380 atattacaaa atatctcact ctgtcgccag caatggtgta atcagcgcag acaaatggcg 25440 taaagatcgc ggaaaaacct ccccgagtgg catgatagct gcctctgtat tgctgattta 25500 gtcagcctta tttgacttaa gggtgccctc gttagtgaca aattgctttc aaggagacag 25560 ccatgcccca cactttgttg aaaaacaaat tgcctttggg gagacggtaa agccagttgc 25620 tcttcaataa ggaatgtcga ggaggcaatg taaccgcctc tggtagtaca cttctctaat 25680 ccaaaaatca atttgtattc aagataccgc aaaaaactta tggtttaaac cctgcaggac 25740 tagtccagaa ggtaattatc caagatgtag catcaagaat ccaatgttta cgggaaaaac 25800 tatggaagta ttatgtaagc tcagcaagaa gcagatcaat atgcggcaca tatgcaacct 25860 atgttcaaaa atgaagaatg tacagataca agatcctata ctgccagaat acgaagaaga 25920 atacgtagaa attgaaaaag aagaaccagg cgaagaaaag aatcttgaag acgtaagcac 25980 tgacgacaac aatgaaaaga agaagataag gtcggtgatt gtgaaagaga catagaggac 26040 acatgtaagg tggaaaatgt aagggcggaa agtaacctta tcacaaagga atcttatccc 26100 ccactactta tccttttata tttttccgtg tcatttttgc ccttgagttt tcctatataa 26160 ggaaccaagt tcggcatttg tgaaaacaag aaaaaatttg gtgtaagcta ttttctttga 26220 agtactgagg atacaacttc agagaaattt gtaagtttgt aggtaccaga tctggatccc 26280 aaaccatgtc tccggagagg agaccagttg agattaggcc agctacagca gctgatatgg 26340 ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc tacagtgaac tttaggacag 26400 agccacaaac accacaagag tggattgatg atctagagag gttgcaagat agataccctt 26460 ggttggttgc tgaggttgag ggtgttgtgg ctggtattgc ttacgctggg ccctggaagg 26520 ctaggaacgc ttacgattgg acagttgaga gtactgttta cgtgtcacat aggcatcaaa 26580 ggttgggcct aggatctaca ttgtacacac atttgcttaa gtctatggag gcgcaaggtt 26640 ttaagtctgt ggttgctgtt ataggccttc caaacgatcc atctgttagg ttgcatgagg 26700 ctttgggata cacagcccgg ggtacattgc gcgcagctgg atacaagcat ggtggatggc 26760 atgatgttgg tttttggcaa agggattttg agttgccagc tcctccaagg ccagttaggc 26820 cagttaccca aatctgagta gttagcttaa tcacctagag ctcgatcggc ggcaatagct 26880 tcttagcgcc atcccgggtt gatcctatct gtgttgaaat agttgcggtg ggcaaggctc 26940 tctttcagaa agacaggcgg ccaaaggaac ccaaggtgag gtgggctatg gctctcagtt 27000 ccttgtggaa gcgcttggtc taaggtgcag aggtgttagc gggatgaagc aaaagtgtcc 27060 gattgtaaca agatatgttg atcctacgta aggatattaa agtatgtatt catcactaat 27120 ataatcagtg tattccaata tgtactacga tttccaatgt ctttattgtc gccgtatgta 27180 atcggcgtca caaaataatc cccggtgact ttcttttaat ccaggatgaa ataatatgtt 27240 attataattt ttgcgatttg gtccgttata ggaattgaag tgtgcttgag gtcggtcgcc 27300 accactccca tttcataatt ttacatgtat ttgaaaaata aaaatttatg gtattcaatt 27360 taaacacgta tacttgtaaa gaatgatatc ttgaaagaaa tatagtttaa atatttattg 27420 ataaaataac aagtcaggta ttatagtcca agcaaaaaca taaatttatt gatgcaagtt 27480 taaattcaga aatatttcaa taactgatta tatcagctgg tacattgccg tagatgaaag 27540 actgagtgcg atattatggt gtaatacata ggaattcgtt taaacgatct gcgtctaatt 27600 ttcggtccaa cttgcacagg aaagacgtcg accgcggtag ctcttgccca gcagactggg 27660 cttccagtcc tttcgctcga tcgggtccaa tgttgtcctc agctgtgaac cggaagcgga 27720 cgaccaacag tggaagaact gaaaggaacg agccgtctat accttgatga tcggcctctg 27780 gtgaagggta tcatcgcagc caagcaagct catgaaaggc tgatggggga ggtgtataat 27840 tatgaggccc acggcgggct tattctttag ggaggatcta tctcgttgct caagtgcatg 27900 gcgcaaagca gttattggag tgcggatttt cgttggcata ttattcgcca cgagttagca 27960 gacgaagaga ccttcatgaa cgtggccaag gccagagtta agcagatgtt acgccctgct 28020 gcaggccttt ctattatcca atagttggtt gatctttgga aagagcctcg gctgaggccc 28080 atactgaaag agatcgatgg atatcgatat gccatgttgt ttgctagcca gaaccagatc 28140 acatccgata tgctattgca gcttgacgca gatatggagg ataagttgat tcatgggatc 28200 gctcaggagt agctcatcca tgcacgccga caagaacaga aattccgtcg agttaacgca 28260 gccgcttacg acggattcga aggtcatcca ttcggaatgt attagtttgc accagctccg 28320 cgtcacacct gtcttcattt gaataagatg ttagcaattg tttttagctt tgtcttgttg 28380 tggcagggcg gcaagtgctt cagacatcat tctgttttca aattttatgc tggagaacag 28440 cttcttaatt cctttggaaa taatagactg cgtcttaaaa ttcagatgtc tggatataga 28500 tatgattgta aaataaccta tttaagtgtc atttagaaca taagttttat gaatgttctt 28560 ccattttcgt catcgaacga ataagagtaa atacaccttt tttaacatta caaataagtt 28620 cttatacgtt gtttatacac cgggaatcat ttccattatt ttcgcgcaaa agtcacggat 28680 attcgtgaaa gcgacataaa ctgcgaaatt tgcggggagt gtcttgagtt tgcctcgagg 28740 ctagcgcatg cacatagaca cacacatcat ctcattgatg cttggtaata attgtcatta 28800 gattgttttt atgcatagat gcactcgaaa tcagccaatt ttagacaagt atcaaacgga 28860 tgtgacttca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt 28920 tgatttacaa ttgaatatat cctgccccag ccagccaaca gctcgattta caattgaata 28980 tatcctgccg gccggcccac gcgtgtcgag gaattctgat ctggccccca tttggacgtg 29040 aatgtagaca cgtcgaaata aagatttccg aattagaata atttgtttat tgctttcgcc 29100 tataaatacg acggatcgta atttgtcgtt ttatcaaaat gtactttcat tttataataa 29160 cgctgcggac atctacattt ttgaattgaa aaaaaattgg taattactct ttctttttct 29220 ccatattgac catcatactc attgctgatc catgtagatt tcccggacat gaagccattt 29280 acaattgaat atatcctgcc gccgctgccg ctttgcaccc ggtggagctt gcatgttggt 29340 ttctacgcag aactgagccg gttaggcaga taatttccat tgagaactga gccatgtgca 29400 ccttcccccc aacacggtga gcgacggggc aacggagtga tccacatggg acttttaaac 29460 atcatccgtc ggatggcgtt gcgagagaag cagtcgatcc gtgagatcag ccgacgcacc 29520 gggcaggcgc gcaacacgat cgcaaagtat ttgaacgcag gtacaatcga gccgacgttc 29580 acgcggaacg accaagcaag cttggctgcc atttttgggg tgaggccgtt cgcggccgag 29640 gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg 29700 gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac 29760 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa 29820 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa 29880 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca 29940 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt 30000 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg 30060 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg 30120 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc 30180 cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc 30240 tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt 30300 gagcgtcgga aagggtcgac ggatcttttc cgctgcataa ccctgcttcg gggtcattat 30360 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt 30420 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg 30480 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa 30540 cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg 30600 gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcaagg tgtactgcct 30660 tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc 30720 ctacctgctg gccgtcggcc agggctacaa aatcacgggc gtcgtggact atgagcacgt 30780 ccgcgagctg gcccgcatca atggcgacct gggccgcctg ggcggcctgc tgaaactctg 30840 gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc 30900 gaagatcgaa gagaagcagg acgagcttgg caaggtcatg atgggcgtgg tccgcccgag 30960 ggcagagcca tgactttttt agccgctaaa acggccgggg ggtgcgcgtg attgccaagc 31020 acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtattc gtgcagggca 31080 agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg 31140 ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc 31200 acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg 31260 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa 31320 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga 31380 tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc 31440 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt 31500 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg 31560 ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca 31620 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca 31680 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga 31740 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga 31800 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt 31860 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt 31920 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt 31980 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg 32040 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg 32100 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat 32160 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc 32220 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca 32280 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg 32340 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg 32400 tggggtcagt tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag 32460 cgggcactgc tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta 32520 cgaactgccg ataaacagag gattaaaatt gacaattgtg attaaggctc agattcgacg 32580 gcttggagcg gccgacgtgc aggatttccg cgagatccga ttgtcggccc tgaagaaagc 32640 tccagagatg ttcgggtccg tttacgagca cgaggagaaa aagcccatgg aggcgttcgc 32700 tgaacggttg cgagatgccg tggcattcgg cgcctacatc gacggcgaga tcattgggct 32760 gtcggtcttc aaacaggagg acggccccaa ggacgctcac aaggcgcatc tgtccggcgt 32820 tttcgtggag cccgaacagc gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc 32880 ggcgggttta ttgctcgtga tgatcgtccg acagattcca acgggaatct ggtggatgcg 32940 catcttcatc ctcggcgcac ttaatatttc gctattctgg agcttgttgt ttatttcggt 33000 ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt 33060 gttcatctct gccgctctgc taggtagccc gatacgattg atggcggtcc tgggggctat 33120 ttgcggaact gcgggcgtgg cgctgttggt gttgacacca aacgcagcgc tagatcctgt 33180 cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg ttcggaaccg tgctgacccg 33240 caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc tggcaactgg cggccggagg 33300 acttctgctc gttccagtag ctttagtgtt tgatccgcca atcccgatgc ctacaggaac 33360 caatgttctc ggcctggcgt ggctcggcct gatcggagcg ggtttaacct acttcctttg 33420 gttccggggg atctcgcgac tcgaacctac agttgtttcc ttactgggct ttctcagccc 33480 ccgagcgctt agtgggaatt tgtacccctt atcgaaccgg gagcacagga tgacgcctaa 33540 caattcattc aagccgacac cgcttcgcgg cgcggcttaa ttcaggagtt aaacatcatg 33600 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag 33660 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc 33720 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca 33780 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag 33840 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat 33900 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc 33960 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat 34020 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat 34080 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc 34140 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa 34200 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag 34260 cccgtcatac ttgaagctag gcaggcttat cttggacaag aagatcgctt ggcctcgcgc 34320 gcagatcagt tggaagaatt tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc 34380 aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc 34440 gttagagagc tggggaagac tatgcgcgat ctgttgaagg tggttctaag cctcgtcttg 34500 cgatggcatt tcgatccatt cccattccgc gctcaagatg gcttcccctc ggcagttcat 34560 cagggctaaa tcaatctagc cgacttgtcc ggtgaaatgg gctgcactcc aacagaaaca 34620 atcaaacaaa catacacagc gacttattca cacgagctca aattacaacg gtatatatcc 34680 tgccagtcag catcatcaca ccaaaagtta ggcccgaata gtttgaaatt agaaagctcg 34740 caattgaggt ctacaggcca aattcgctct tagccgtaca atattactca ccggatccta 34800 accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc gcacaagttt 34850 <210> 63 <211> 35154 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9151 <400> 63 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctagga ctaggcactg agtgactcac 240 tgacctgact gagtgactgg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggcg cttcgtgtca agaccaacaa gaagccatgt tgggagatga 900 ccaaggagga actgacctct ggcaaaactg aggtgttcaa ctatgaggaa ctcctggagt 960 ttgcagaggg tgacatagct aaggtttttg gtccggagtt tgctgtcata gacaagtacc 1020 ccagaagggt gaggctgcct gctcgtgaat acctgcttgt gacaagggtc acactcatgg 1080 atgccgaggt caacaactac agagttggtg ccagaatggt cactgagtat gatcttccag 1140 tgaatggcga attgtctgag ggaggtgatt gtccctgggc agttctggtt gagagtggac 1200 agtgcgattt gatgctgata tcatacatgg gaattgattt ccagaaccaa ggtgacagag 1260 tttaccgtct gctcaacacc acgttgacat tctatggggt tgcccatgag ggagagaccc 1320 ttgagtatga catcagagtg accggttttg ccaaacgttt ggatggtggc atctccatgt 1380 tcttctttga gtatgactgc tatgtcaatg ggaggcttct cattgagatg agagatggct 1440 gtgctggctt cttcacaaat gaggagcttg atgcgggaaa gggtgtggtg ttcacaagag 1500 gggatttggc tgccagagct aagattccaa agcaagatgt ttctccctat gctgtcgctc 1560 cctgccttca caaaacaaaa ctcaacgaaa aggagatgca gaccttggtt gacaaagatt 1620 gggcttccgt ttttggctca aagaatggca tgccggagat caactacaaa ctctgcgcac 1680 gtaagatgct catgattgat agagtcacca gcattgacca taagggaggt gtgtacggtt 1740 tgggtcagtt ggttggtgag aaaatcttgg agagggacca ttggtacttc ccatgccatt 1800 ttgttaagga tcaagtcatg gctggatctc ttgtcagcga cggttgttca caaatgctca 1860 agatgtacat gatctggctc ggtttgcacc ttaccaccgg accatttgac ttcagacccg 1920 tcaacggaca cccgaacaaa gttaggtgta ggggtcagat atctcctcat aagggaaagt 1980 tggtctacgt catggagatc aaagagatgg gattcgacga ggataacgat ccgtacgcca 2040 ttgctgacgt caacatcatt gacgttgatt tcgaaaaggg tcaagatttc agccttgata 2100 gaatcagcga ctacggaaag ggggatctta acaaaaagat cgtcgtggac ttcaagggca 2160 ttgctctcaa gatgcagaag agatccacga acaagaaccc ctccaaagtt cagcccgttt 2220 ttgctaacgg tgcagccact gtgggaccgg aagcgtccaa ggccagctct ggtgccagtg 2280 ctagtgcttc tgctgcccct gcgaagcctg ctttctcagc agatgttctt gcgccaaagc 2340 cagttgcact tcctgaacat atcctcaagg gtgacgctct cgctccgaaa gaaatgtcct 2400 ggcatcccat gtcaaaactt cctggcaacc cgacgccgtt cttctcccct agttcttacc 2460 ctccgagggc aatttgcttc atccctttcc ctggcaaccc acttgacaac aattgcaaag 2520 ctggagaaat gcctctgaac tggtacaaca tgtcagagtt catgtgtgga aaagttagca 2580 actgcttggg accagagttc gcaagatttg ataagtcaaa tacgagcaga agccctgctt 2640 ttgatttggc tcttgtgact agagttgttg aagtcacaaa catggaacat ggcaagttcc 2700 tgaatgttga ttgcaatccc agcaaaggca caatggtggg ggagtttgac tgtcctcaag 2760 atgcgtggtt ctttgatggt tcttgcaatg atgggcacat gccttattcc atcatcatgg 2820 aaatcggact tcagacatcc ggtgttctca ccagtgtgtt gaaagcacca ctgacgatgg 2880 acaaagatga cattctcttt aggaaccttg atgcaagtgc tgaaatggtg aggccagatg 2940 tggatgtgag ggggaaaacc ataaggaatg tgaccaagtg cactggatat gcaatgttgg 3000 gaaagatggg gattcacaga ttcacgtttg agttgtctgt tgatggcgtg gttttctaca 3060 aaggaagcac ttcctttgga tggttcactc cagaggtgtt tgctcagcaa gctggacttg 3120 acaatgggaa aaagacggag ccttggtgca aaactaacaa cacgagtgtg agaagagttg 3180 aaatcgcatc agccaaaggc aaagaacagc tgactgagaa acttccagat gcaactaatg 3240 ctcaagttct cagaaggtca gaacagtgtg agtatttgga ttacctcaac attgctcctg 3300 attctgggct gcatgggaaa ggatatgcgc atggacacaa agatgttaac ccacaagact 3360 ggttcttctc ttgtcacttt tggtttgatc ctgtgatgcc tggatctttg ggaattgagt 3420 caatgttcca gctcattgag gcatttgcgg tggaccagaa cataccggga gaatacaatg 3480 tgagcaatcc gacctttgcc catgcccctg gaaaaacggc gtggaagtac cgtggtcaat 3540 tgacaccaaa gaaccgtgcg atggactgtg aggtgcacat agtttcaatc acagcctctc 3600 ctgaaaatgg aggctacgtt gacatagtgg cagatggagc actttgggtt gatggtctcc 3660 gtgtgtatga agccaaagaa ctcagagttc gtgttgtcag tgcaaaacct caagccattc 3720 cggatgttca acaacagcca cccagcgcaa aagcggaccc tgggaaaact ggagttgcac 3780 tcagtccgac tcagttgaga gatgtcctgc ttgaagtgga caatccattg tatcttggtg 3840 ttgaaaactc caatttggtg cagtttgaat ccaaacctgc aacttcttca aggattgtca 3900 gtatcaaacc atgctccata agtgatcttg gtgacaagtc cttcatggaa acgtacaatg 3960 tgtcagcacc tctgtacact ggagcaatgg ccaagggaat tgcgtctgcg gaccttgtca 4020 ttgctgctgg gaagaggaag atattgggtt cttttggagc gggtgggctt ccaatgcacc 4080 atgtgagggc agctttggag aagatacaag cggctctgcc tcaaggacca tatgctgtga 4140 acctcattca ttcacctttt gacagcaact tggaaaaggg gaatgttgat ctcttcctcg 4200 aaaagggcgt tactgtggtg gaggcttctg cattcatgac cctcactccc caagttgttc 4260 gttaccgtgc agccggactc tcaaggaacg ctgatgggag tgtcaacatc agaaaccgta 4320 tcattggaaa agtgtccaga acagaactcg ccgaaatgtt catcagaccg gctccggaac 4380 acctccttga gaaactcata gcttctggtg agataacaca agaacaagcc gaacttgccc 4440 gtcgtgttcc tgtcgcagac gacatcgctg ttgaagctga ttctggtggt cacactgaca 4500 accgtcccat ccacgtcatc ttgcctttga tcatcaacct taggaacaga ctgcacagag 4560 agtgtggtta tcctgcgcac cttagagtca gagttggagc tggaggcggt gttggctgcc 4620 cacaagctgc tgcggctgcc ctcacaatgg gagctgcctt catagtgact gggactgtca 4680 atcaagttgc taaacagtcc gggacatgtg acaacgtgag aaaacaactc agtcaagcca 4740 cctacagtga catttgcatg gctccagcag ctgatatgtt tgaggaaggt gtgaaactcc 4800 aagttctcaa gaagggaaca atgttcccat caagagctaa caaattgtac gaactctttt 4860 gcaagtacga ctctttcgat tcaatgcctc ctgctgaact tgaaaggatc gagaagagga 4920 tcttcaagag ggcacttcaa gaggtctggg aagagaccaa ggacttttac atcaatggtt 4980 tgaagaatcc agagaagata cagagagcgg aacatgaccc caaactcaag atgtctttgt 5040 gtttcagatg gtatcttggt cttgcatcac gttgggccaa catgggtgct cctgatcgtg 5100 tcatggacta tcaagtctgg tgtggtccag ccattggagc cttcaatgac ttcatcaaag 5160 gaacatatct tgatcctgct gtctctaatg agtacccatg tgttgtccag atcaaccttc 5220 agattttgcg tggtgcatgt tacctgaggc gtcttaatgc tctgagaaat gatccgagga 5280 ttgatcttga gactgaagat gctgcctttg tctatgagcc aacgaatgcg ctttgagtag 5340 ttagcttaat cacttaggtc acccagtcca agcccggcct agcacgatga tcatgagtgt 5400 tgctattgat gtatgttaat actatacaca gtcatcgtgt gtgttttata aaactgtaag 5460 cttaggatgt ttgaggctaa tgtaattagc actacaccgt aataaatgag aggtttactt 5520 tatgttttaa taatgttctt acccttgctg ctctctaaac tgtaatggcg tacggttagc 5580 taaactaaag taaaaatcga ttcgcttgag taaaattagt gaccgggaaa gatcatcgac 5640 tactactgct cagagatgta atcggtaatt aagacttcaa gctcaataat taattaggag 5700 taaatctggc ttcgaatttt taaattgtca acttccaaag aaggattggc aagactcaat 5760 ctcaattgcc caacaaacat atggctcttt acaaatttgg aatctctttt tgatggcaag 5820 aacgtcgtaa ttcctctgtc gatctcatgt aattaccaaa ctcatctact tattactttc 5880 tttatatgca cttactgatg cggccgctta attaatcacg agtggatgac tgtcacgact 5940 gactcatgac tgactactag tgctagcctc gaggtcgaca caactttgta tacaaaagtt 6000 gtctagacct aggctcagca actcatccag tcaagttcga gtcagtgagt tgggcggccg 6060 cagcttatag taaagatttt tttgttaata aaatgaaagt taatatttat ggatttattt 6120 gaacatattt acaagttttt gttaataaat ttatttacag aagtgttaaa tttgaaaatg 6180 tgttaaatga gagttaatat ttatggatat aatattactt tatttaaaaa taaaatgaat 6240 ttatattaaa atatgatttg gtaaaagata ttataaagag gaacttggat gagaaagacg 6300 acgatgggca aaagttgcat ggcacaccgc ttttgttcag taacatctgc actgtggcgc 6360 ctttttcaca ctcaccatgc atgctgccac gtcagctttc acactatgac accactggcc 6420 atacacgttg ccacctcagc tttctcctct tctcactatg acacgactgg ccatgcatgc 6480 atgcatgctg ccacctcagc tcccgcctct tcacccgtgt ctttctctca tgtcgcagtg 6540 ctgcgtgcca acctcttctc tctataaata gagagaagag aatgatggtt aaactcaaac 6600 caaaacaaga aaacatacac aaatagcaaa acggatccac aaccaaaaat ggctgcaagg 6660 aacgtgagtg cggctcatga gatgcatgac gagaagagga tagctgttgt gggcatggct 6720 gttcagtatg ctggatgcaa aacgaaagac gagttctggg aagttctcat gaatggaaaa 6780 gttgagagca aagtgatcag cgacaaacgt cttggttcaa attaccgtgc tgaacactac 6840 aaagctgaaa ggagcaagta tgctgacacc ttctgcaatg agacatatgg aactcttgat 6900 gagaacgaaa tagacaatga gcatgaactc cttttgaacc ttgccaaaca agccttggct 6960 gaaacatctg tcaaagattc aacaagatgt ggcatagttt ctggatgcct cagcttcccg 7020 atggacaacc tccaaggtga actcttgaat gtgtaccaga accatgttga gaagaaactt 7080 ggtgccagag ttttcaaaga tgcctctcac tggtcagaaa gagaacagtc caacaaacca 7140 gaggctggtg acagaagaat cttcatggac cctgcttcct ttgttgctga ggaactcaat 7200 cttggcgctc tccactactc tgttgatgct gcctgtgcca ctgcgctcta tgtgctcaga 7260 ctggcgcaag atcacttggt ctctggtgca gctgatgtga tgctttgtgg tgcaacatgc 7320 ctgccggagc cgtttttcat actctctggg ttctcaacct tccaagccat gcctgttgga 7380 acgggtcaga atgtgtccat gccgctgcac aaagattctc aaggattgac ccctggtgaa 7440 ggaggttcca tcatggtcct caaacgtttg gatgacgcaa tcagagatgg agatcacatc 7500 tatggaacac ttttgggagc caatgtcagc aacagtggca ctggtctgcc acttaaaccc 7560 cttttgccgt cagagaaaaa gtgcctcatg gacacctaca cacgtatcaa tgtgcatccc 7620 cacaagattc agtatgttga atgccatgca actgggactc cacaaggtga tcgtgtggaa 7680 attgatgctg tgaaggcttg ctttgaaggc aaagtcccac gttttggaac cacaaaaggg 7740 aactttggac acacattggt ggcagctggg tttgctggca tgtgcaaagt gcttctctcc 7800 atgaagcacg ggatcattcc acccactccg ggaatagatg acgaaacgaa gatggaccct 7860 ctggttgtct ctggtgaggc cattccctgg ccagaaacga atggagaacc gaagcgtgct 7920 ggtctctcag cgtttggatt tggtgggacc aatgctcatg ctgtctttga ggaacatgac 7980 ccaagcaatg ctgcatgcac tggccatgac tccatttctg ctctctctgc acgttgtggt 8040 ggtgagtcaa acatgagaat agcaatcact gggatggatg ccacttttgg tgctttgaaa 8100 ggattggatg cttttgaaag ggccatctac actggtgctc atggtgctat accactccca 8160 gaaaagagat ggagattcct ggggaaagac aaggacttcc ttgatctttg tggagtcaaa 8220 gcaacaccgc atggctgtta cattgaagat gttgaagttg atttccagag gctgaggact 8280 ccaatgactc ctgaagatat gctcttgcct caacagcttc tggctgtcac cacaattgat 8340 agggccatat tggactctgg catgaagaaa ggtggaaatg tggctgtctt tgtggggttg 8400 gggaccgatc ttgaactcta ccgtcaccgt gcaagggttg ctctcaagga gagagtgagg 8460 cctgaagcca gcaagaaact caatgacatg atgcagtaca tcaatgattg tgggacaagc 8520 acttcttaca ccagctacat aggcaacttg gttgcaacaa gggtcagttc acaatggggc 8580 ttcacgggac cttccttcac aatcacagaa ggcaacaatt ctgtgtacag atgtgctgaa 8640 cttggcaaat acctcttgga aacgggagag gttgatgggg ttgtggttgc gggtgttgat 8700 ctctgtggga gtgctgagaa cctttacgtc aagtcaaggc gtttcaaggt gtctacatct 8760 gacaccccga gagccagctt tgatgcagct gctgatggtt actttgttgg agagggctgt 8820 ggtgcatttg tgctcaagcg tgagacaagc tgcaccaagg atgacagaat ctatgcttgc 8880 atggatgcca tagttcctgg aaatgttcca tctgcatgtt tgagagaggc attggatcaa 8940 gccagagtca agcctggaga catcgagatg ttggagcttt ctgctgattc agcgaggcat 9000 ttgaaggacc cgtctgttct cccaaaggaa ctcactgctg aggaagagat tggcggactt 9060 cagacgattc ttcgtgacga tgacaagctc ccaaggaatg ttgcaactgg aagtgtcaag 9120 gccactgttg gtgacactgg ttatgcttct ggtgctgcca gtttgattaa ggctgcgctt 9180 tgcatctaca accgttacct gcccagcaat ggagatgact gggatgaacc tgcacctgaa 9240 gcaccctggg acagcacact ctttgcgtgc cagacctcaa gagcttggtt gaagaaccct 9300 ggtgagagac gttatgcggc tgtttccgga gtcagtgaaa ccagaagttg ctacagtgtg 9360 ctgctttctg aagcagaggg acactacgag agagagaaca gaatcagttt ggatgaggaa 9420 gcacccaagc tcattgtgtt gagagcagac tcccatgaag agattcttgg aagattggac 9480 aagataaggg aaaggttctt gcagcccacg ggtgctgcac ccagagagtc tgaattgaag 9540 gcgcaagccc gtaggatctt tcttgaactc cttggtgaga cccttgccca agatgcagct 9600 tcatctggaa gtcagaagcc ccttgctctc agcttggtct ccactccatc caagctccag 9660 agagaggtgg aacttgcggc aaaaggcatt ccgagatgtc tcaagatgag acgtgattgg 9720 agttcccctg ctggaagcag atatgcgcct gagcctttgg ccagtgacag agttgccttc 9780 atgtatggag aaggaaggag cccttactat ggcatcacac aagacattca taggatttgg 9840 cctgaacttc atgaggtcat caatgagaaa acaaaccgtc tctgggctga aggtgacaga 9900 tgggtcatgc caagggccag cttcaagagt gagcttgagt cacaacagca agagtttgac 9960 agaaacatga ttgaaatgtt ccgtcttgga atcctcacct caattgcctt cacaaacctg 10020 gcgagagatg ttctcaacat cacgcccaaa gcagcctttg gactcagttt gggagagatt 10080 tccatgatct ttgctttctc caagaaaaac ggtttgataa gcgatcagtt gacaaaggat 10140 ttgagagagt cagatgtctg gaacaaagct ctggctgttg agttcaatgc gctcagagaa 10200 gcctggggca ttccacagtc tgtcccaaaa gatgagttct ggcaaggtta catagtgagg 10260 ggaacgaaac aagacataga agcagccata gcaccagact caaagtatgt gaggttgacc 10320 atcatcaatg atgccaacac tgctctcatc agtggaaagc ctgatgcatg caaagctgcg 10380 attgctcgtc ttggtggcaa catccctgcg cttccagtca ctcaaggcat gtgtgggcat 10440 tgcccagagg tgggacctta caccaaggac atcgcaaaga tccacgccaa ccttgagttc 10500 cctgtggttg atggtcttga tctctggact accataaacc agaagaggct tgtgccaaga 10560 gccacgggtg ccaaagatga atgggctcct tcatcctttg gggagtatgc tggacagctc 10620 tatgagaaac aagccaactt ccctcagata gttgaaacca tctacaagca gaactatgat 10680 gtgtttgttg aagttgggcc aaacaatcac agaagcacag cagtgagaac cacgcttgga 10740 ccacagagaa accaccttgc tggtgccata gacaagcaga acgaggatgc ttggacgaca 10800 atagtcaagc tcgtggcttc attgaaggct caccttgttc ctggagtgac catcagtccg 10860 ctctaccact ccaagctcgt tgcagaggct gaggcttgtt atgctgcgct ctgtaaaggt 10920 gagaaaccca agaaaaacaa gtttgtgaga aagattcagt tgaatggcag attcaacagc 10980 aaggcagacc ccatctcctc agcagacctt gcatccttcc cacctgcgga ccctgcgatt 11040 gaagctgcca tctcttcaag gatcatgaag cctgtggctc cgaaattcta tgcgcgtctc 11100 aacattgatg agcaagatga gaccagagat ccgatcttga acaaggacaa tgcgccttcc 11160 agctccagct cttccagcag ttcctcttcc agcagttcat ctccgtctcc tgctccttca 11220 gctccagttc agaagaaagc tgccccagct gcggagacaa aagctgttgc cagtgctgat 11280 gcacttcgta gtgccctgct tgatcttgac tccatgcttg cgctcagctc tgcaagtgct 11340 tctggcaacc ttgttgagac tgcgccatct gatgccagtg tcattgtccc tccgtgcaac 11400 attgctgatc ttggcagcag agctttcatg aaaacttatg gtgtttctgc gcctctgtac 11460 accggtgcaa tggccaaggg aattgcctct gcggacttgg tgatcgcagc tggaaggcaa 11520 ggaatccttg cgtcatttgg tgctggaggc cttccaatgc aagttgtgcg tgagtccatt 11580 gaaaagattc aagctgccct gcccaatgga ccatatgctg tcaacctcat ccattctcct 11640 tttgacagca accttgagaa gggaaatgtt gatctcttct tggaaaaggg tgtcactttt 11700 gttgaggctt cagccttcat gacgttgaca ccgcaagttg tcagatacag agcggctggg 11760 ttgacgagga atgcagatgg gtcagtcaac ataaggaaca gaatcattgg gaaagtctcc 11820 agaactgagc tggctgaaat gttcatgcgt cctgcacctg agcatctttt gcagaagctc 11880 attgcttctg gggagatcaa tcaagaacaa gctgagttgg ccagacgtgt tcctgtggct 11940 gatgacattg cggttgaagc tgattctggt ggacacactg acaaccgtcc aatccatgtc 12000 attctgcctc tgattatcaa cctgagggat aggcttcaca gagagtgtgg atacccagca 12060 aacctgaggg ttcgtgttgg tgctgggggt ggcattgggt gtccccaagc tgcgctggca 12120 actttcaaca tgggtgcctc tttcattgtc actggaacag tgaaccaagt tgccaaacaa 12180 tctgggacgt gtgacaatgt gaggaaacag cttgcgaaag caacttactc agatgtttgc 12240 atggcaccgg cagctgacat gtttgaggaa ggtgtgaaac ttcaagtttt gaagaaagga 12300 acaatgttcc cttcaagagc caacaaactc tatgagttgt tctgcaagta tgattctttt 12360 gagtccatgc ctccagcaga gcttgcgagg gttgagaaaa gaatcttcag tcgtgcattg 12420 gaagaggtct gggatgagac caagaacttc tacatcaacc gtctccacaa cccagagaag 12480 atacagaggg ctgagaggga ccccaaactg aagatgtcac tgtgtttcag atggtatctg 12540 tccctggctt caaggtgggc caacactgga gcttctgaca gagtcatgga ctaccaagtt 12600 tggtgtggtc ctgccattgg ttcattcaat gatttcatca aaggaactta ccttgatccg 12660 gcagtggcaa atgagtaccc gtgtgttgtg cagatcaaca aacagattct tagaggagcg 12720 tgcttcttga ggcgtcttga aatactgaga aatgcaaggc tttcagatgg tgcagctgca 12780 cttgtggcga gcattgatga cacatatgtc cctgctgaga agctgtgagt agttagctta 12840 atcacttagg tcacccagtc caagcccggc ctagcacgat gatcatgagt gttgctattg 12900 atgtatgtta atactataca cagtcatcgt gtgtgtttta taaaactgta agcttaggat 12960 gtttgaggct aatgtaatta gcactacacc gtaataaatg agaggtttac tttatgtttt 13020 aataatgttc ttacccttgc tgctctctaa actgtaatgg cgtacggtta gctaaactaa 13080 agtaaaaatc gattcgcttg agtaaaatta gtgaccggga aagatcatcg actactactg 13140 ctcagagatg taatcggtaa ttaagacttc aagctcaata attaattagg agtaaatctg 13200 gcttcgaatt tttaaattgt caacttccaa agaaggattg gcaagactca atctcaattg 13260 cccaacaaac atatggctct ttacaaattt ggaatctctt tttgatggca agaacgtcgt 13320 aattcctctg tcgatctcat gtaattacca aactcatcta cttattactt tctttatatg 13380 cacttactga tgcggccgct taattaatct gacctgacca ctagacctga gtgagtacag 13440 atgtgctact agtgctagcc tcgaggtcga ccaacttttc tatacaaagt tgtctagacc 13500 taggagctgt gctgggtaac tcacatgagt cactggtcac atcagcggcc gcagcttata 13560 gtaaagattt ttttgttaat aaaatgaaag ttaatattta tggatttatt tgaacatatt 13620 tacaagtttt tgttaataaa tttatttaca gaagtgttaa atttgaaaat gtgttaaatg 13680 agagttaata tttatggata taatattact ttatttaaaa ataaaatgaa tttatattaa 13740 aatatgattt ggtaaaagat attataaaga ggaacttgga tgagaaagac gacgatgggc 13800 aaaagttgca tggcacaccg cttttgttca gtaacatctg cactgtggcg cctttttcac 13860 actcaccatg catgctgcca cgtcagcttt cacactatga caccactggc catacacgtt 13920 gccacctcag ctttctcctc ttctcactat gacacgactg gccatgcatg catgcatgct 13980 gccacctcag ctcccgcctc ttcacccgtg tctttctctc atgtcgcagt gctgcgtgcc 14040 aacctcttct ctctataaat agagagaaga gaatgatggt taaactcaaa ccaaaacaag 14100 aaaacataca caaatagcaa aacggatcca caaccaaaaa tgcttcagca cacttggctt 14160 ccgaagcctc ccaatctgac cctcttgtca gatgaggttc atctctggag gattcctctt 14220 gaccagcctg agtcacaact tcaagacctt gctgccaccc tgagcagtga tgaattggcg 14280 agggcaaaca gattctactt tccagaacac agaaggcgtt tcactgctgg gagaggcatc 14340 ctcagatcca tcttgggtgg atacttggga gtggaaccgg gtcaagtcaa gtttgattat 14400 gagtcccgtg ggaaaccgat ccttggtgac agatttgctg agagtggact cctgttcaac 14460 ttgtctcaca gccagaacct tgccttgtgt gctgtcaact acacgcgtca aataggcatt 14520 gatcttgaat atctgcgtcc aacatctgac ttggagtctc ttgcaaagag gttctttctc 14580 ccaagagaat atgaactctt gaggtcactc cctgatgagc agaaacagaa gattttcttt 14640 cgttactgga cttgcaaaga ggcttatctc aaagcaacgg gagatggaat agccaaactt 14700 gaagagatcg agatagcact caccccaaca gaacctgcca agctccaaac agctcctgcg 14760 tggtctctgt tggagcttgt gccagatgac aattgtgttg cagctgtggc tgttgcgggt 14820 tttggttggc agcccaagtt ctggcattac tgagtagtta gcttaatcac ttaggtcacc 14880 cagtccaagc ccggcctagc acgatgatca tgagtgttgc tattgatgta tgttaatact 14940 atacacagtc atcgtgtgtg ttttataaaa ctgtaagctt aggatgtttg aggctaatgt 15000 aattagcact acaccgtaat aaatgagagg tttactttat gttttaataa tgttcttacc 15060 cttgctgctc tctaaactgt aatggcgtac ggttagctaa actaaagtaa aaatcgattc 15120 gcttgagtaa aattagtgac cgggaaagat catcgactac tactgctcag agatgtaatc 15180 ggtaattaag acttcaagct caataattaa ttaggagtaa atctggcttc gaatttttaa 15240 attgtcaact tccaaagaag gattggcaag actcaatctc aattgcccaa caaacatatg 15300 gctctttaca aatttggaat ctctttttga tggcaagaac gtcgtaattc ctctgtcgat 15360 ctcatgtaat taccaaactc atctacttat tactttcttt atatgcactt actgatgcgg 15420 ccgcttaatt aactactgtc actgaggccg tagacgagta cggactgatc taactagtgc 15480 tagcctcgag gtcgacacaa ctttgtataa taaagttgtc tagacctagg tggagtcatc 15540 acgcagacta tctcagcatg tgcgtagcac gcggccgcag cttatagtaa agattttttt 15600 gttaataaaa tgaaagttaa tatttatgga tttatttgaa catatttaca agtttttgtt 15660 aataaattta tttacagaag tgttaaattt gaaaatgtgt taaatgagag ttaatattta 15720 tggatataat attactttat ttaaaaataa aatgaattta tattaaaata tgatttggta 15780 aaagatatta taaagaggaa cttggatgag aaagacgacg atgggcaaaa gttgcatggc 15840 acaccgcttt tgttcagtaa catctgcact gtggcgcctt tttcacactc accatgcatg 15900 ctgccacgtc agctttcaca ctatgacacc actggccata cacgttgcca cctcagcttt 15960 ctcctcttct cactatgaca cgactggcca tgcatgcatg catgctgcca cctcagctcc 16020 cgcctcttca cccgtgtctt tctctcatgt cgcagtgctg cgtgccaacc tcttctctct 16080 ataaatagag agaagagaat gatggttaaa ctcaaaccaa aacaagaaaa catacacaaa 16140 tagcaaaacg gatccacaac caaaaatggc agcgaggctg caagagcaga aaggaggtga 16200 gatggacacg aggattgcga tcataggcat gagcgccata cttccttgtg gaaccacggt 16260 gagagagtca tgggagacca taagggctgg cattgattgc ctgtctgatc ttcctgagga 16320 tcgtgttgat gtgactgcgt actttgaccc agtcaagacc acaaaggaca aaatctactg 16380 caagagagga ggtttcattc ctgagtatga ctttgatgcg cgtgagtttg gtctcaacat 16440 gttccagatg gaagattctg atgcgaatca gaccatctca cttctcaagg tcaaagaagc 16500 acttcaagat gctgggattg atgcacttgg aaaggagaag aaaaacatag gatgcgtgct 16560 tggcattgga ggtggacaga agagttcaca tgagttctac agcagactca actatgtggt 16620 tgtggagaaa gtcttgagaa agatggggat gccagaggaa gatgtcaaag tggctgtgga 16680 gaagtacaaa gcgaacttcc cagaatggag acttgactct ttccctggat tccttgggaa 16740 tgtcactgct ggcagatgca ccaacacatt caacttggat ggcatgaact gcgttgtgga 16800 tgctgcatgt gcaagctcct tgatagcagt caaggttgcg attgatgaac tgctctatgg 16860 tgactgcgac atgatggtca ctggtgcaac ctgcacagac aactccattg ggatgtacat 16920 ggctttctca aagacacctg tgttcagcac ggacccaagt gtgagagcgt atgatgagaa 16980 aacgaaaggg atgctcattg gtgagggatc agccatgttg gtgctcaaac gttatgcaga 17040 tgcagtgagg gatggtgatg agattcatgc tgtgattcgt ggttgtgcaa gctcaagtga 17100 tggcaaagca gctggcatct acactcccac aatcagtggt caagaggaag ccttgagacg 17160 tgcatacaat cgtgcctgcg ttgaccctgc cactgtcact ttggttgagg gtcatgggac 17220 tggcactcca gttggagata ggattgagtt gactgcgttg aggaacttgt ttgacaaagc 17280 gtatggtgaa gggaacacag agaaagttgc agttggaagt atcaagtcca gtattggtca 17340 tttgaaggct gttgctggtc ttgctggaat gatcaaggtc atcatggctc tcaaacacaa 17400 gactttgcct ggaacgatca atgtggacaa tccacccaat ctctatgaca acactcccat 17460 caatgagtca tccctctaca tcaacacgat gaacagaccc tggttccctc cacctggtgt 17520 tccgagacgt gctggaatat ccagctttgg ctttggaggt gccaactacc atgcagtgtt 17580 ggaagaggct gaaccagagc acacgacagc gtacagactc aacaaacgtc cacaaccggt 17640 gttgatgatg gctgcaactc ctgctgcgtt gcagagcctt tgtgaagctc agctgaaaga 17700 gtttgaagct gcgatcaagg agaatgagac agtcaagaat actgcctaca tcaagtgtgt 17760 caagtttgga gaacagttca agttccctgg gagcatacct gcgaccaatg cgagactggg 17820 attcttggtc aaagatgcgg aggatgcgtg ctccacgctt cgtgctatat gtgctcagtt 17880 tgccaaagat gtgaccaaag aagcgtggag gcttccgaga gaaggagttt ctttccgtgc 17940 caaaggcatt gccaccaatg gtgctgttgc tgcactcttc tctggacaag gtgctcagta 18000 cacacacatg ttcagtgaag ttgcgatgaa ctggcctcag ttcagacagt ccattgctgc 18060 aatggacgct gcacagtcca aggttgctgg aagtgacaaa gactttgaaa gagtctctca 18120 agtcctgtac ccaaggaaac cgtacgaacg tgagccagaa caagatcaca agaaaatctc 18180 actcacagca tacagccagc cgtcaaccct ggcatgtgct cttggtgcct ttgagatttt 18240 caaggaagct ggattcactc cagactttgc agctggacac agcttgggtg agtttgctgc 18300 gctctatgca gctggttgtg ttgacagaga tgagttgttt gaacttgttt gcagaagggc 18360 aaggatcatg ggaggcaaag atgcacctgc cacacccaaa ggttgcatgg cagctgtcat 18420 aggacccaac gcagagaaca tcaaggttca agctgccaac gtctggcttg gaaactcaaa 18480 ctctccgtct cagactgtca ttactgggtc tgttgaaggg attcaagctg aaagtgccag 18540 attgcagaag gaagggttca gagttgtgcc tcttgcttgt gagtctgcct tccattctcc 18600 acagatggaa aatgccagct cagctttcaa agatgtcata tccaaggttt ccttccgtac 18660 acccaaagca gaaacaaagc tcttcagcaa tgtttctgga gagacctatc cgacggatgc 18720 cagagagatg ctcactcaac acatgacctc atctgtgaag ttcctgaccc aagtgaggaa 18780 catgcaccaa gctggtgcga gaatctttgt tgagtttgga ccgaaacaag ttctcagcaa 18840 acttgtttct gaaaccctca aagatgaccc gtctgttgtc acagtgtctg tgaacccagc 18900 ttctggcacg gactctgaca tacagttgag ggatgctgca gttcagctgg ttgtggctgg 18960 tgtcaatctt caagggtttg acaagtggga tgctcctgat gccacgagga tgcaagccat 19020 caagaaaaag agaaccacac tcagactttc agctgccacc tatgtcagtg acaagaccaa 19080 gaaagtgagg gatgctgcca tgaacgacgg aagatgtgtc acatacctca agggtgctgc 19140 accactcatc aaggctcctg aacctgtggt tgacgaagct gccaagaggg aggctgaaag 19200 gcttcagaag gagcttcaag acgctcagag gcagcttgac gacgccaaac gtgcagctgc 19260 agaagccaac tccaaacttg ctgctgccaa ggaggaagcc aagactgctg cagcttcagc 19320 caagcctgca gttgacactg ctgttgtgga gaaacatcgt gccatcctca agtccatgct 19380 ggctgagctg gatggttatg gaagtgttga tgcttcttcc cttcagcaac agcaacagca 19440 acagactgct ccggcgcctg ttaaagccgc tgcccctgcc gctcccgttg caagtgctcc 19500 tgctcccgcc gtgtccaacg agttgcttga gaaagctgag acagttgtga tggaggttct 19560 cgctgccaag actggatacg aaacagacat gattgaggcg gacatggagc ttgagactga 19620 gttgggaatt gacagcatca agagggttga aattctttct gaagtccaag ctatgcttaa 19680 cgttgaggcg aaagatgttg atgctctctc taggacgaga acagtgggtg aagttgtcaa 19740 cgctatgaag gcagaaatcg ctggttcttc cgctccggcg ccggcagctg ccgcacctgc 19800 accggctaaa gcggctccag ctgcagctgc accagctgtt tctaatgagc tgctcgaaaa 19860 ggcagaaact gttgtgatgg aagtccttgc tgccaaaact ggctacgaaa ctgacatgat 19920 agaatcagac atggagcttg agacggagct tggaattgat tccatcaagc gtgtggaaat 19980 cctcagtgaa gtccaagcca tgctcaatgt ggaagctaag gatgtggatg ctctctcaag 20040 gaccagaact gtcggtgagg ttgtcaatgc catgaaggct gagatagctg gaggctccgc 20100 tcctgcgccg gccgctgccg ctcctggtcc cgctgccgcg gctccagctc cagctgcagc 20160 cgctccggcc gtctcaaacg aactccttga aaaagctgag accgtcgtga tggaggtctt 20220 ggcagctaaa accggctatg agactgacat gattgagtct gacatggaat tggagacaga 20280 acttgggata gacagcatca aacgtgttga aatcctcagt gaggtccaag ctatgctgaa 20340 cgtggaagcg aaggatgttg atgctctgtc cagaacgagg accgttggtg aggttgtgga 20400 tgccatgaag gctgaaattg ctggagggtc tgcgcccgcg ccagctgcag ccgctcctgc 20460 acccgcagcc gcggcacctg ctccagctgc acctgcacct gccgtttctt cagaactctt 20520 ggagaaggct gagacggttg tgatggaggt tttggctgca aagactggct atgagaccga 20580 catgattgaa tctgatatgg aattggagac agagcttggg atcgattcca tcaaaagagt 20640 ggagatcctg agtgaagtcc aagccatgct caatgttgaa gctaaggatg ttgatgcact 20700 ttcaagaaca aggacagtgg gtgaggttgt ggatgccatg aaggcggaga ttgctggagg 20760 gagcgcgcct gcgccagctg ccgcggcacc tgcaccggca gccgcagctc cagctccagc 20820 tgcaccagca ccggccgcgc ccgcgccagc tgtttcatcc gaactcctgg aaaaggccga 20880 gacggttgtg atggaggtgc tcgcagctaa gactggttac gagaccgaca tgattgaaag 20940 tgacatggag cttgaaaccg agttgggaat agatagcatc aaacgtgttg aaatcttgtc 21000 tgaggtccaa gctatgttga acgtggaggc aaaagatgtc gatgcgcttt caagaaccag 21060 aacagtcggt gaggtcgtgg acgccatgaa ggctgaaatt gccggaagct ctgctagtgc 21120 gccagctgca gccgcacctg cacctgctgc cgcggcacct gctccagctg cagccgctcc 21180 tgccgtcagc aatgagttgc tggagaaagc cgaaacagtt gtgatggaag tccttgctgc 21240 aaaaactggt tacgaaacgg acatgattga gtcagacatg gaattggaga ccgaacttgg 21300 aatagattcc atcaagagag tggagatcct ctctgaggtg caagccatgc tcaacgttga 21360 ggctaaagat gttgatgcac tcagtaggac aagaactgtg ggggaagttg tcgatgcgat 21420 gaaggctgag attgctggag gctctgctcc tgcgccggca gctgccgcac ctgcacccgc 21480 agccgcggca ccagctgtca gcaacgaatt gcttgagaag gctgaaacag ttgtcatgga 21540 agtgctggct gcaaaaaccg gctatgagac tgacatgatt gagtctgaca tggaacttga 21600 gacggaattg ggcattgaca gcatcaagcg tgttgagatc ctcagtgaag tccaagcaat 21660 gctcaatgtg gaggctaagg atgtggatgc cctctcaagg accagaacag ttggtgaggt 21720 cgttgatgcg atgaaggcgg agatcgctgg aagttccgcg cctgcgccag ctgcagccgc 21780 tcctgcaccc gcagccgcgg caccggctcc agctgcagcc gcaccggccg tctcctcaga 21840 actcctggaa aaggctgaga cagttgtgat ggaagtcctt gctgcgaaaa ctggctatga 21900 gaccgacatg attgaatctg acatggaact ggagaccgag cttggaattg actccatcaa 21960 acgtgttgaa atcctctctg aggttcaagc gatgttgaat gtggaggcca aagatgttga 22020 tgctctttcc agaacaagga cggtgggaga ggtggttgat gccatgaagg ctgagatagc 22080 tgggggttct gctcctgcgc cagctgcagc cgctcctgct cccgctgccg cggcaccagc 22140 tgtcagcaac gaactccttg aaaaagctga aactgttgtg atggaggttt tggctgcaaa 22200 gactggatac gagactgaca tgatagagtc agacatggag ttggaaacag agcttgggat 22260 tgatagcatc aagcgtgtgg aaatcctttc tgaggttcaa gccatgctga acgttgaggc 22320 caaagatgtc gatgctttgt caaggaccag aacggttgga gaagtggtcg atgccatgaa 22380 ggctgagata gctggtggat cagcccctgc tccggctgca gccgctcctg caagtgccgg 22440 tgctgcacca gctgtgaaga ttgattcagt tcatggtgct gactgtgacg atttgtcact 22500 gatgcacgca aaggtggttg acatcagaag gccagatgaa ctgattctgg aaagaccgga 22560 gaaccgtcca gttcttgtcg ttgacgatgg ctcagagttg acacttgctc ttgtgagggt 22620 gttgggagcc tgtgcagttg tcttgacctt tgagggattg cagttggctc agagagctgg 22680 tgcagctgcc attcgtcatg ttctggctaa ggacctttca gcagaatcag ctgagaaggc 22740 catcaaagag gctgagcaaa gatttggagc gttgggaggc ttcataagcc agcaagcaga 22800 aaggtttgaa ccagctgaga tactgggttt cacactcatg tgtgcgaagt ttgccaaagc 22860 ctctctctgc actgctgtgg ctggaggtcg tcctgccttc atcggtgtgg ctaggcttga 22920 cggcagactt ggattcacct ctcaaggcac ttctgacgca ctcaagagag cacagcgtgg 22980 agcgatcttt ggactctgca agacaatagg gttggagtgg tcagaatctg atgtgttcag 23040 ccgtggtgtg gacattgctc aagggatgca cccagaagat gctgcagttg ccatagttag 23100 ggagatggcg tgtgctgaca tcagaatcag agaggttggg attggagcaa accagcaaag 23160 atgcacaatc agagctgcca agttggagac tgggaaccct cagagacaga tagcgaaaga 23220 cgatgtgctt ctggtttctg gaggtgctcg tgggatcaca cctctttgca tccgtgagat 23280 cacgcgtcag attgctggag gcaagtacat tctgttgggt cgtagcaaag tttctgcgtc 23340 tgaaccagca tggtgtgctg gcatcactga tgagaaggct gtgcagaaag ctgccaccca 23400 agagttgaag agggccttca gtgctggtga aggacccaaa cccactccaa gggctgtcac 23460 gaaacttgtg ggatctgttc ttggtgctcg tgaagtgaga agctccattg ctgccattga 23520 agctctgggt ggcaaagcga tctacagctc ttgtgatgtg aactctgctg cagatgttgc 23580 caaagcagtt agggatgcag agtcccagct tggtgccaga gttagtggaa tcgttcacgc 23640 ttctggtgtg ctccgtgatc gtctcataga gaagaaactc cctgacgagt ttgatgctgt 23700 ctttgggacc aaagtcactg ggttggagaa tcttctggct gcagttgatc gtgccaacct 23760 caaacacatg gttctcttca gctcccttgc tggtttccac ggcaatgttg gtcagtctga 23820 ctatgcgatg gcaaacgaag ctctcaacaa gatgggtttg gaacttgcga aggatgtcag 23880 tgtcaagtcc atctgctttg gtccctggga tggtgggatg gtgacacctc aactcaagaa 23940 acagtttcaa gaaatgggag ttcagatcat tcctcgtgaa ggaggtgctg atacagttgc 24000 gaggattgtg ttgggatcaa gtcctgctga gatccttgtt ggaaactgga ggacaccatc 24060 caagaaagtt ggaagtgaca cgatcaccct gcatagaaag atcagtgcca agtccaatcc 24120 cttcttggag gatcatgtca tacaaggcag aagggtgttg ccaatgaccc ttgctattgg 24180 gagccttgct gagacctgcc ttggactctt ccctggctac tctctctggg ccatagatga 24240 cgcacagctc ttcaaaggtg tcactgttga tggagacgtc aactgtgaag tgaccctcac 24300 accgtccact gctccgagtg ggagggtgaa tgtccaagcg acgctcaaga cgttctcaag 24360 tggaaaactt gttccagcct acagagctgt catagtgttg tccaaccaag gtgctccacc 24420 tgccaatgca accatgcagc ctccgagcct tgatgcagat cctgctttgc aagggtcagt 24480 ctatgatggc aagactctct ttcatggtcc tgcgttcaga ggcattgatg acgttctcag 24540 ctgcaccaag agccagcttg ttgccaagtg ttctgctgtt cctggatcag atgcagccag 24600 aggagagttt gcaactgaca ctgatgcaca tgatcccttt gtcaatgatc ttgcctttca 24660 agcaatgttg gtctgggtga ggagaacgct gggacaagct gcactcccga actccattca 24720 gaggatagtc caacacagac cggtcccaca agacaaaccc ttctacatca ccctcagatc 24780 caatcagagt ggtggacata gccagcacaa acatgcactt cagttccaca acgaacaagg 24840 tgatctcttc attgatgttc aagcctctgt cattgcgaca gactcacttg cgttctgagt 24900 agttagctta atcacttagg tcacccagtc caagcccggc ctagcacgat gatcatgagt 24960 gttgctattg atgtatgtta atactataca cagtcatcgt gtgtgtttta taaaactgta 25020 agcttaggat gtttgaggct aatgtaatta gcactacacc gtaataaatg agaggtttac 25080 tttatgtttt aataatgttc ttacccttgc tgctctctaa actgtaatgg cgtacggtta 25140 gctaaactaa agtaaaaatc gattcgcttg agtaaaatta gtgaccggga aagatcatcg 25200 actactactg ctcagagatg taatcggtaa ttaagacttc aagctcaata attaattagg 25260 agtaaatctg gcttcgaatt tttaaattgt caacttccaa agaaggattg gcaagactca 25320 atctcaattg cccaacaaac atatggctct ttacaaattt ggaatctctt tttgatggca 25380 agaacgtcgt aattcctctg tcgatctcat gtaattacca aactcatcta cttattactt 25440 tctttatatg cacttactga tgcggccgct taattaagat gagtgatact caggactcag 25500 gactcactct gctgatcact agtgctagcc tcgaggtcga cacccagctt tcttgtacaa 25560 agtggttgcg gccgcttaat taaatttaaa ttcaattaat gcaatcttga ttttcaacaa 25620 cgaaggtaat ggcgtaaaag aaaaaatgta tgttattgta ttgatctttc atgatgttga 25680 agcgtgccat aatatgatga tgtataatta aaatattaac tgtcgcattt tattgaaatg 25740 gcactgttat ttcaaccata tctttgattc tgttacatga cacgactgca agaagtaaat 25800 aatagacgcc gttgttaaag aattgctatc atatgtgcct aactagaggg aatttgagcg 25860 tcagacctaa tcaaatatta caaaatatct cactctgtcg ccagcaatgg tgtaatcagc 25920 gcagacaaat ggcgtaaaga tcgcggaaaa acctccccga gtggcatgat agctgcctct 25980 gtattgctga tttagtcagc cttatttgac ttaagggtgc cctcgttagt gacaaattgc 26040 tttcaaggag acagccatgc cccacacttt gttgaaaaac aaattgcctt tggggagacg 26100 gtaaagccag ttgctcttca ataaggaatg tcgaggaggc aatgtaaccg cctctggtag 26160 tacacttctc taatccaaaa atcaatttgt attcaagata ccgcaaaaaa cttatggttt 26220 aaaccctgca ggactagtcc agaaggtaat tatccaagat gtagcatcaa gaatccaatg 26280 tttacgggaa aaactatgga agtattatgt aagctcagca agaagcagat caatatgcgg 26340 cacatatgca acctatgttc aaaaatgaag aatgtacaga tacaagatcc tatactgcca 26400 gaatacgaag aagaatacgt agaaattgaa aaagaagaac caggcgaaga aaagaatctt 26460 gaagacgtaa gcactgacga caacaatgaa aagaagaaga taaggtcggt gattgtgaaa 26520 gagacataga ggacacatgt aaggtggaaa atgtaagggc ggaaagtaac cttatcacaa 26580 aggaatctta tcccccacta cttatccttt tatatttttc cgtgtcattt ttgcccttga 26640 gttttcctat ataaggaacc aagttcggca tttgtgaaaa caagaaaaaa tttggtgtaa 26700 gctattttct ttgaagtact gaggatacaa cttcagagaa atttgtaagt ttgtaggtac 26760 cagatctgga tcccaaacca tgtctccgga gaggagacca gttgagatta ggccagctac 26820 agcagctgat atggccgcgg tttgtgatat cgttaaccat tacattgaga cgtctacagt 26880 gaactttagg acagagccac aaacaccaca agagtggatt gatgatctag agaggttgca 26940 agatagatac ccttggttgg ttgctgaggt tgagggtgtt gtggctggta ttgcttacgc 27000 tgggccctgg aaggctagga acgcttacga ttggacagtt gagagtactg tttacgtgtc 27060 acataggcat caaaggttgg gcctaggatc tacattgtac acacatttgc ttaagtctat 27120 ggaggcgcaa ggttttaagt ctgtggttgc tgttataggc cttccaaacg atccatctgt 27180 taggttgcat gaggctttgg gatacacagc ccggggtaca ttgcgcgcag ctggatacaa 27240 gcatggtgga tggcatgatg ttggtttttg gcaaagggat tttgagttgc cagctcctcc 27300 aaggccagtt aggccagtta cccaaatctg agtagttagc ttaatcacct agagctcgat 27360 cggcggcaat agcttcttag cgccatcccg ggttgatcct atctgtgttg aaatagttgc 27420 ggtgggcaag gctctctttc agaaagacag gcggccaaag gaacccaagg tgaggtgggc 27480 tatggctctc agttccttgt ggaagcgctt ggtctaaggt gcagaggtgt tagcgggatg 27540 aagcaaaagt gtccgattgt aacaagatat gttgatccta cgtaaggata ttaaagtatg 27600 tattcatcac taatataatc agtgtattcc aatatgtact acgatttcca atgtctttat 27660 tgtcgccgta tgtaatcggc gtcacaaaat aatccccggt gactttcttt taatccagga 27720 tgaaataata tgttattata atttttgcga tttggtccgt tataggaatt gaagtgtgct 27780 tgaggtcggt cgccaccact cccatttcat aattttacat gtatttgaaa aataaaaatt 27840 tatggtattc aatttaaaca cgtatacttg taaagaatga tatcttgaaa gaaatatagt 27900 ttaaatattt attgataaaa taacaagtca ggtattatag tccaagcaaa aacataaatt 27960 tattgatgca agtttaaatt cagaaatatt tcaataactg attatatcag ctggtacatt 28020 gccgtagatg aaagactgag tgcgatatta tggtgtaata cataggaatt cgtttaaacg 28080 atctgcgtct aattttcggt ccaacttgca caggaaagac gtcgaccgcg gtagctcttg 28140 cccagcagac tgggcttcca gtcctttcgc tcgatcgggt ccaatgttgt cctcagctgt 28200 gaaccggaag cggacgacca acagtggaag aactgaaagg aacgagccgt ctataccttg 28260 atgatcggcc tctggtgaag ggtatcatcg cagccaagca agctcatgaa aggctgatgg 28320 gggaggtgta taattatgag gcccacggcg ggcttattct ttagggagga tctatctcgt 28380 tgctcaagtg catggcgcaa agcagttatt ggagtgcgga ttttcgttgg catattattc 28440 gccacgagtt agcagacgaa gagaccttca tgaacgtggc caaggccaga gttaagcaga 28500 tgttacgccc tgctgcaggc ctttctatta tccaatagtt ggttgatctt tggaaagagc 28560 ctcggctgag gcccatactg aaagagatcg atggatatcg atatgccatg ttgtttgcta 28620 gccagaacca gatcacatcc gatatgctat tgcagcttga cgcagatatg gaggataagt 28680 tgattcatgg gatcgctcag gagtagctca tccatgcacg ccgacaagaa cagaaattcc 28740 gtcgagttaa cgcagccgct tacgacggat tcgaaggtca tccattcgga atgtattagt 28800 ttgcaccagc tccgcgtcac acctgtcttc atttgaataa gatgttagca attgttttta 28860 gctttgtctt gttgtggcag ggcggcaagt gcttcagaca tcattctgtt ttcaaatttt 28920 atgctggaga acagcttctt aattcctttg gaaataatag actgcgtctt aaaattcaga 28980 tgtctggata tagatatgat tgtaaaataa cctatttaag tgtcatttag aacataagtt 29040 ttatgaatgt tcttccattt tcgtcatcga acgaataaga gtaaatacac cttttttaac 29100 attacaaata agttcttata cgttgtttat acaccgggaa tcatttccat tattttcgcg 29160 caaaagtcac ggatattcgt gaaagcgaca taaactgcga aatttgcggg gagtgtcttg 29220 agtttgcctc gaggctagcg catgcacata gacacacaca tcatctcatt gatgcttggt 29280 aataattgtc attagattgt ttttatgcat agatgcactc gaaatcagcc aattttagac 29340 aagtatcaaa cggatgtgac ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg 29400 tctaagcgtc aatttgattt acaattgaat atatcctgcc ccagccagcc aacagctcga 29460 tttacaattg aatatatcct gccggccggc ccacgcgtgt cgaggaattc tgatctggcc 29520 cccatttgga cgtgaatgta gacacgtcga aataaagatt tccgaattag aataatttgt 29580 ttattgcttt cgcctataaa tacgacggat cgtaatttgt cgttttatca aaatgtactt 29640 tcattttata ataacgctgc ggacatctac atttttgaat tgaaaaaaaa ttggtaatta 29700 ctctttcttt ttctccatat tgaccatcat actcattgct gatccatgta gatttcccgg 29760 acatgaagcc atttacaatt gaatatatcc tgccgccgct gccgctttgc acccggtgga 29820 gcttgcatgt tggtttctac gcagaactga gccggttagg cagataattt ccattgagaa 29880 ctgagccatg tgcaccttcc ccccaacacg gtgagcgacg gggcaacgga gtgatccaca 29940 tgggactttt aaacatcatc cgtcggatgg cgttgcgaga gaagcagtcg atccgtgaga 30000 tcagccgacg caccgggcag gcgcgcaaca cgatcgcaaa gtatttgaac gcaggtacaa 30060 tcgagccgac gttcacgcgg aacgaccaag caagcttggc tgccattttt ggggtgaggc 30120 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg 30180 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag 30240 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag 30300 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc 30360 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag 30420 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt 30480 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg 30540 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc 30600 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg 30660 agggccaagt tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc 30720 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg 30780 gcaaccagcc cggtgagcgt cggaaagggt cgacggatct tttccgctgc ataaccctgc 30840 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga 30900 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg 30960 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac 31020 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc cggcgtaaca 31080 gatgagggca agcggatggc tgatgaaacc aagccaacca ggaagggcag cccacctatc 31140 aaggtgtact gccttccaga cgaacgaaga gcgattgagg aaaaggcggc ggcggccggc 31200 atgagcctgt cggcctacct gctggccgtc ggccagggct acaaaatcac gggcgtcgtg 31260 gactatgagc acgtccgcga gctggcccgc atcaatggcg acctgggccg cctgggcggc 31320 ctgctgaaac tctggctcac cgacgacccg cgcacggcgc ggttcggtga tgccacgatc 31380 ctcgccctgc tggcgaagat cgaagagaag caggacgagc ttggcaaggt catgatgggc 31440 gtggtccgcc cgagggcaga gccatgactt ttttagccgc taaaacggcc ggggggtgcg 31500 cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag agcgacttcg cggagctggt 31560 attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg 31620 gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa 31680 tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat 31740 gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc 31800 cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca 31860 gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact 31920 ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca 31980 ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa 32040 gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc 32100 gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc 32160 gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac 32220 cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa 32280 caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt 32340 gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac 32400 gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc 32460 cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg 32520 gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac 32580 gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta 32640 cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga 32700 ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg 32760 cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga 32820 gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa 32880 acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg ctttactggc 32940 atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc gctcgggacg 33000 cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat tgtgattaag 33060 gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat ccgattgtcg 33120 gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga gaaaaagccc 33180 atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta catcgacggc 33240 gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc tcacaaggcg 33300 catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc cggtatgctg 33360 ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat tccaacggga 33420 atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt ctggagcttg 33480 ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag 33540 ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg attgatggcg 33600 gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac accaaacgca 33660 gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat ggcgttcgga 33720 accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac cgcctggcaa 33780 ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc gccaatcccg 33840 atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg agcgggttta 33900 acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt ttccttactg 33960 ggctttctca gcccccgagc gcttagtggg aatttgtacc ccttatcgaa ccgggagcac 34020 aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg 34080 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 34140 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 34200 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 34260 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 34320 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 34380 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 34440 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 34500 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 34560 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 34620 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 34680 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 34740 cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc 34800 gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca 34860 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc 34920 ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg aaggtggttc 34980 taagcctcgt cttgcgatgg catttcgatc cattcccatt ccgcgctcaa gatggcttcc 35040 cctcggcagt tcatcagggc taaatcaatc tagccgactt gtccggtgaa atgggctgca 35100 ctccaacaga aacaatcaaa caaacataca cagcgactta ttcacacgag ctca 35154 <210> 64 <211> 38074 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB108207 <400> 64 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga 900 ggattgcgat cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat 960 gggagaccat aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg 1020 tgactgcgta ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag 1080 gtttcattcc tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg 1140 aagattctga tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg 1200 ctgggattga tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag 1260 gtggacagaa gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag 1320 tcttgagaaa gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag 1380 cgaacttccc agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg 1440 gcagatgcac caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg 1500 caagctcctt gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca 1560 tgatggtcac tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa 1620 agacacctgt gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga 1680 tgctcattgg tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg 1740 atggtgatga gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag 1800 ctggcatcta cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc 1860 gtgcctgcgt tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag 1920 ttggagatag gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag 1980 ggaacacaga gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg 2040 ttgctggtct tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg 2100 gaacgatcaa tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat 2160 ccctctacat caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg 2220 ctggaatatc cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg 2280 aaccagagca cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg 2340 ctgcaactcc tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg 2400 cgatcaagga gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag 2460 aacagttcaa gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca 2520 aagatgcgga ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg 2580 tgaccaaaga agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg 2640 ccaccaatgg tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt 2700 tcagtgaagt tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg 2760 cacagtccaa ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc 2820 caaggaaacc gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat 2880 acagccagcc gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg 2940 gattcactcc agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag 3000 ctggttgtgt tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg 3060 gaggcaaaga tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg 3120 cagagaacat caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc 3180 agactgtcat tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg 3240 aagggttcag agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa 3300 atgccagctc agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag 3360 aaacaaagct cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc 3420 tcactcaaca catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag 3480 ctggtgcgag aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg 3540 aaaccctcaa agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg 3600 actctgacat acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc 3660 aagggtttga caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga 3720 gaaccacact cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg 3780 atgctgccat gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca 3840 aggctcctga acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg 3900 agcttcaaga cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact 3960 ccaaacttgc tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag 4020 ttgacactgc tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg 4080 atggttatgg aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc 4140 cggcgcctgt taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg 4200 tgtccaacga gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga 4260 ctggatacga aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg 4320 acagcatcaa gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga 4380 aagatgttga tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg 4440 cagaaatcgc tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag 4500 cggctccagc tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg 4560 ttgtgatgga agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca 4620 tggagcttga gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag 4680 tccaagccat gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg 4740 tcggtgaggt tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg 4800 ccgctgccgc tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg 4860 tctcaaacga actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa 4920 ccggctatga gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag 4980 acagcatcaa acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga 5040 aggatgttga tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg 5100 ctgaaattgc tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg 5160 cggcacctgc tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg 5220 agacggttgt gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat 5280 ctgatatgga attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga 5340 gtgaagtcca agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa 5400 ggacagtggg tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg 5460 cgccagctgc cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac 5520 cggccgcgcc cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga 5580 tggaggtgct cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc 5640 ttgaaaccga gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag 5700 ctatgttgaa cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg 5760 aggtcgtgga cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag 5820 ccgcacctgc acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca 5880 atgagttgct ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt 5940 acgaaacgga catgattgag tcagacatgg aattggagac cgaacttgga atagattcca 6000 tcaagagagt ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg 6060 ttgatgcact cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga 6120 ttgctggagg ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac 6180 cagctgtcag caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg 6240 caaaaaccgg ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg 6300 gcattgacag catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg 6360 aggctaagga tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga 6420 tgaaggcgga gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg 6480 cagccgcggc accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa 6540 aggctgagac agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga 6600 ttgaatctga catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa 6660 tcctctctga ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca 6720 gaacaaggac ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg 6780 ctcctgcgcc agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg 6840 aactccttga aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg 6900 agactgacat gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca 6960 agcgtgtgga aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg 7020 atgctttgtc aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag 7080 ctggtggatc agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag 7140 ctgtgaagat tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa 7200 aggtggttga catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag 7260 ttcttgtcgt tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct 7320 gtgcagttgt cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca 7380 ttcgtcatgt tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg 7440 ctgagcaaag atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac 7500 cagctgagat actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca 7560 ctgctgtggc tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg 7620 gattcacctc tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg 7680 gactctgcaa gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg 7740 acattgctca agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt 7800 gtgctgacat cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca 7860 gagctgccaa gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc 7920 tggtttctgg aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga 7980 ttgctggagg caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat 8040 ggtgtgctgg catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga 8100 gggccttcag tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg 8160 gatctgttct tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg 8220 gcaaagcgat ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta 8280 gggatgcaga gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc 8340 tccgtgatcg tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca 8400 aagtcactgg gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg 8460 ttctcttcag ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg 8520 caaacgaagc tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca 8580 tctgctttgg tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag 8640 aaatgggagt tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt 8700 tgggatcaag tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg 8760 gaagtgacac gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg 8820 atcatgtcat acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg 8880 agacctgcct tggactcttc cctggctact ctctctgggc catagatgac gcacagctct 8940 tcaaaggtgt cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg 9000 ctccgagtgg gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg 9060 ttccagccta cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa 9120 ccatgcagcc tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca 9180 agactctctt tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga 9240 gccagcttgt tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg 9300 caactgacac tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg 9360 tctgggtgag gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc 9420 aacacagacc ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg 9480 gtggacatag ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca 9540 ttgatgttca agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa 9600 tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga 9660 tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg 9720 tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta 9780 ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa 9840 gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc 9900 tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg 9960 cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc 10020 ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta 10080 attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc 10140 acttactgat gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctagacc 10260 taggctcagc aactcatcca gtcaagttcg agtcagtgag ttgggcggcc gcgaattcgg 10320 cgcgccaacc ttggactccc atgttggcaa aggcaaccaa acaaacaatg aatgatccgc 10380 tcctgcatat ggggcggttt gagtatttca actgccattt gggctgaatt gaagacatgc 10440 tcctgtcaga aattccgtga tcttactcaa tattcagtaa tctcggccaa tatcctaaat 10500 gtgcgtggct ttatctgtct ttgtattgtt tcatcaattc atgtaacgtt tgcttttctt 10560 atgaattttc aaataaatta tcgatagtac tacgaatatt tcgtatcgct gatcttctca 10620 atcacaatga tgcgtagtga cccgacaaat aatttaagcg tccttaatac caatcctaaa 10680 ataattgagg caaataaaat ttttttgtaa tttttatgat agcagatcga ttctccagca 10740 agcctgcaac aaaatattgt gtatttctaa atagattttg atactcgagg tgaccgaatt 10800 cgttggtagg gtgctaggaa acttgttttt ggggttttgt ataagggttg aaacatccct 10860 gaagtgtctc attttatttt atttattctt tgctgataaa aaaataaaat aaaagaagct 10920 aagcacacgg tcaaccattg ctctactgct aaaagggtta tgtgtagtgt tttactgcat 10980 aaattatgca gcaaacaaga caactcaaat taaaaaattt cctttgcttg tttttttgtt 11040 gtctctgact tgactttctt gtggaagttg gttgtataag gattgggaca ccattgtcct 11100 tcttaattta attttattct ttgctgataa aaaaaaaaaa tttcatatag tgttaaataa 11160 taatttgtta aataaccaaa aagtcaaata tgtttactct cgtttaaata attgagattc 11220 gttccagcaa ggctaaacga ttgtatagat ttatgacaat atttactttt ttatagataa 11280 atgttatatt ataataaatt tatatacata tattatatgt tatttattat ttattattat 11340 tttaaatcct tcaatatttt atcaaaccaa ctcataattt tttttttatc tgtaagaagc 11400 aataaaatta aatagaccca ctttaaggat gatccaacct ttatacagag taagagagtt 11460 caaatagtac cttttcatat acatatcaac taaaatatta gaaatatcat ggatcaaacc 11520 ttataaagac attaaataag tggataagta taatatataa atgggtagta tataatatat 11580 aaatggatac aaacttctct ctttataatt gttatgtctc cttaacatcc taatataata 11640 cataagtggg taatatataa tatataaatg gagacaaact tcttccatta taattgttat 11700 gtcttcttaa cacttatgtc tcgttcacaa tgctaaagtt agaattgttt agaaagtctt 11760 atagtacaca tttgtttttg tactatttga agcattccat aagccgtcac gattcagatg 11820 atttataata ataagaggaa atttatcata gaacaataag gtgcatagat agagtgttaa 11880 tatatcataa catcctttgt ttattcatag aagaagtgag atggagctca gttattatac 11940 tgttacatgg tcggatacaa tattccatgc tctccatgag ctcttacacc tacatgcatt 12000 ttagttcata ctggtgacct aagtgattaa gctaactact cagtaatgcc agaacttggg 12060 ctgccaacca aaacccgcaa cagccacagc tgcaacacaa ttgtcatctg gcacaagctc 12120 caacagagac cacgcaggag ctgtttggag cttggcaggt tctgttgggg tgagtgctat 12180 ctcgatctct tcaagtttgg ctattccatc tcccgttgct ttgagataag cctctttgca 12240 agtccagtaa cgaaagaaaa tcttctgttt ctgctcatca gggagtgacc tcaagagttc 12300 atattctctt gggagaaaga acctctttgc aagagactcc aagtcagatg ttggacgcag 12360 atattcaaga tcaatgccta tttgacgcgt gtagttgaca gcacacaagg caaggttctg 12420 gctgtgagac aagttgaaca ggagtccact ctcagcaaat ctgtcaccaa ggatcggttt 12480 cccacgggac tcataatcaa acttgacttg acccggttcc actcccaagt atccacccaa 12540 gatggatctg aggatgcctc tcccagcagt gaaacgcctt ctgtgttctg gaaagtagaa 12600 tctgtttgcc ctcgccaatt catcactgct cagggtggca gcaaggtctt gaagttgtga 12660 ctcaggctgg tcaagaggaa tcctccagag atgaacctca tctgacaaga gggtcagatt 12720 gggaggcttc ggaagccaag tgtgctgaag catttttggt tgtggatcct agagtagtat 12780 tgaatatgag ttgggttggg gtattatagt agtagagtag tagtactctg gatggatgga 12840 tgatgaaaga agtgagtgat attagaggta tttataggta ttatataaga gagaaggtgg 12900 ttggaacatg catggagatt tgggcatggg atgacacgca tatgcaggtt gacgtgtgtt 12960 gaagtgaaga aattgaggtg gcggaagaga atgaatatat acaggtggtt gtggtgatga 13020 tgaagaaaaa ggcaatgtgt ttgtgtgtgg gttgagatgg gtgagccatt taaagtgcat 13080 gttaagcacg tgttgctttg catggcattt agacatacat ggacgcggcg atcttgatca 13140 gccagtgact aatttgagtt ggttgtgtga ttgcgttttg tctctctgtt ttgtcttttt 13200 tctttgttct ttgtcttttt cttgcgcaag atccatgcat gaaccaaaag accacagagt 13260 gtcatggcaa cccacagtaa ttccagttac ggacttacat accaagaaaa ggtaaaagca 13320 ataagaaata tatgaaaatt agtccaccat aaatcttata gtttatggtt tagggtaaca 13380 ctctaacact ctactacatt acttatattt actttaaact atctataaaa caatttaaac 13440 atagtagaat aataaatcaa tagtcacaaa ttcaacaatt aaacttaaaa ttaaaaaagt 13500 aatattttaa ttatatctaa ttaatttttt agaagtaata ttgagtattt gatatatgaa 13560 atcttgaata tagtaactat tattaaaatt actttagaag atgtgtctcg catgtaaaag 13620 cagatctaat ctgacctgac cactagacct gagtgagtac agatgtgcta ctagtgctag 13680 cctcgaggtc gaccaacttt tctatacaaa gttgtctaga cctaggacta ggcactgagt 13740 gactcactga cctgactgag tgactggcgg ccgcagctta tagtaaagat ttttttgtta 13800 ataaaatgaa agttaatatt tatggattta tttgaacata tttacaagtt tttgttaata 13860 aatttattta cagaagtgtt aaatttgaaa atgtgttaaa tgagagttaa tatttatgga 13920 tataatatta ctttatttaa aaataaaatg aatttatatt aaaatatgat ttggtaaaag 13980 atattataaa gaggaacttg gatgagaaag acgacgatgg gcaaaagttg catggcacac 14040 cgcttttgtt cagtaacatc tgcactgtgg cgcctttttc acactcacca tgcatgctgc 14100 cacgtcagct ttcacactat gacaccactg gccatacacg ttgccacctc agctttctcc 14160 tcttctcact atgacacgac tggccatgca tgcatgcatg ctgccacctc agctcccgcc 14220 tcttcacccg tgtctttctc tcatgtcgca gtgctgcgtg ccaacctctt ctctctataa 14280 atagagagaa gagaatgatg gttaaactca aaccaaaaca agaaaacata cacaaatagc 14340 aaaacggatc cacaaccaaa aatggcgctt cgtgtcaaga ccaacaagaa gccatgttgg 14400 gagatgacca aggaggaact gacctctggc aaaactgagg tgttcaacta tgaggaactc 14460 ctggagtttg cagagggtga catagctaag gtttttggtc cggagtttgc tgtcatagac 14520 aagtacccca gaagggtgag gctgcctgct cgtgaatacc tgcttgtgac aagggtcaca 14580 ctcatggatg ccgaggtcaa caactacaga gttggtgcca gaatggtcac tgagtatgat 14640 cttccagtga atggcgaatt gtctgaggga ggtgattgtc cctgggcagt tctggttgag 14700 agtggacagt gcgatttgat gctgatatca tacatgggaa ttgatttcca gaaccaaggt 14760 gacagagttt accgtctgct caacaccacg ttgacattct atggggttgc ccatgaggga 14820 gagacccttg agtatgacat cagagtgacc ggttttgcca aacgtttgga tggtggcatc 14880 tccatgttct tctttgagta tgactgctat gtcaatggga ggcttctcat tgagatgaga 14940 gatggctgtg ctggcttctt cacaaatgag gagcttgatg cgggaaaggg tgtggtgttc 15000 acaagagggg atttggctgc cagagctaag attccaaagc aagatgtttc tccctatgct 15060 gtcgctccct gccttcacaa aacaaaactc aacgaaaagg agatgcagac cttggttgac 15120 aaagattggg cttccgtttt tggctcaaag aatggcatgc cggagatcaa ctacaaactc 15180 tgcgcacgta agatgctcat gattgataga gtcaccagca ttgaccataa gggaggtgtg 15240 tacggtttgg gtcagttggt tggtgagaaa atcttggaga gggaccattg gtacttccca 15300 tgccattttg ttaaggatca agtcatggct ggatctcttg tcagcgacgg ttgttcacaa 15360 atgctcaaga tgtacatgat ctggctcggt ttgcacctta ccaccggacc atttgacttc 15420 agacccgtca acggacaccc gaacaaagtt aggtgtaggg gtcagatatc tcctcataag 15480 ggaaagttgg tctacgtcat ggagatcaaa gagatgggat tcgacgagga taacgatccg 15540 tacgccattg ctgacgtcaa catcattgac gttgatttcg aaaagggtca agatttcagc 15600 cttgatagaa tcagcgacta cggaaagggg gatcttaaca aaaagatcgt cgtggacttc 15660 aagggcattg ctctcaagat gcagaagaga tccacgaaca agaacccctc caaagttcag 15720 cccgtttttg ctaacggtgc agccactgtg ggaccggaag cgtccaaggc cagctctggt 15780 gccagtgcta gtgcttctgc tgcccctgcg aagcctgctt tctcagcaga tgttcttgcg 15840 ccaaagccag ttgcacttcc tgaacatatc ctcaagggtg acgctctcgc tccgaaagaa 15900 atgtcctggc atcccatgtc aaaacttcct ggcaacccga cgccgttctt ctcccctagt 15960 tcttaccctc cgagggcaat ttgcttcatc cctttccctg gcaacccact tgacaacaat 16020 tgcaaagctg gagaaatgcc tctgaactgg tacaacatgt cagagttcat gtgtggaaaa 16080 gttagcaact gcttgggacc agagttcgca agatttgata agtcaaatac gagcagaagc 16140 cctgcttttg atttggctct tgtgactaga gttgttgaag tcacaaacat ggaacatggc 16200 aagttcctga atgttgattg caatcccagc aaaggcacaa tggtggggga gtttgactgt 16260 cctcaagatg cgtggttctt tgatggttct tgcaatgatg ggcacatgcc ttattccatc 16320 atcatggaaa tcggacttca gacatccggt gttctcacca gtgtgttgaa agcaccactg 16380 acgatggaca aagatgacat tctctttagg aaccttgatg caagtgctga aatggtgagg 16440 ccagatgtgg atgtgagggg gaaaaccata aggaatgtga ccaagtgcac tggatatgca 16500 atgttgggaa agatggggat tcacagattc acgtttgagt tgtctgttga tggcgtggtt 16560 ttctacaaag gaagcacttc ctttggatgg ttcactccag aggtgtttgc tcagcaagct 16620 ggacttgaca atgggaaaaa gacggagcct tggtgcaaaa ctaacaacac gagtgtgaga 16680 agagttgaaa tcgcatcagc caaaggcaaa gaacagctga ctgagaaact tccagatgca 16740 actaatgctc aagttctcag aaggtcagaa cagtgtgagt atttggatta cctcaacatt 16800 gctcctgatt ctgggctgca tgggaaagga tatgcgcatg gacacaaaga tgttaaccca 16860 caagactggt tcttctcttg tcacttttgg tttgatcctg tgatgcctgg atctttggga 16920 attgagtcaa tgttccagct cattgaggca tttgcggtgg accagaacat accgggagaa 16980 tacaatgtga gcaatccgac ctttgcccat gcccctggaa aaacggcgtg gaagtaccgt 17040 ggtcaattga caccaaagaa ccgtgcgatg gactgtgagg tgcacatagt ttcaatcaca 17100 gcctctcctg aaaatggagg ctacgttgac atagtggcag atggagcact ttgggttgat 17160 ggtctccgtg tgtatgaagc caaagaactc agagttcgtg ttgtcagtgc aaaacctcaa 17220 gccattccgg atgttcaaca acagccaccc agcgcaaaag cggaccctgg gaaaactgga 17280 gttgcactca gtccgactca gttgagagat gtcctgcttg aagtggacaa tccattgtat 17340 cttggtgttg aaaactccaa tttggtgcag tttgaatcca aacctgcaac ttcttcaagg 17400 attgtcagta tcaaaccatg ctccataagt gatcttggtg acaagtcctt catggaaacg 17460 tacaatgtgt cagcacctct gtacactgga gcaatggcca agggaattgc gtctgcggac 17520 cttgtcattg ctgctgggaa gaggaagata ttgggttctt ttggagcggg tgggcttcca 17580 atgcaccatg tgagggcagc tttggagaag atacaagcgg ctctgcctca aggaccatat 17640 gctgtgaacc tcattcattc accttttgac agcaacttgg aaaaggggaa tgttgatctc 17700 ttcctcgaaa agggcgttac tgtggtggag gcttctgcat tcatgaccct cactccccaa 17760 gttgttcgtt accgtgcagc cggactctca aggaacgctg atgggagtgt caacatcaga 17820 aaccgtatca ttggaaaagt gtccagaaca gaactcgccg aaatgttcat cagaccggct 17880 ccggaacacc tccttgagaa actcatagct tctggtgaga taacacaaga acaagccgaa 17940 cttgcccgtc gtgttcctgt cgcagacgac atcgctgttg aagctgattc tggtggtcac 18000 actgacaacc gtcccatcca cgtcatcttg cctttgatca tcaaccttag gaacagactg 18060 cacagagagt gtggttatcc tgcgcacctt agagtcagag ttggagctgg aggcggtgtt 18120 ggctgcccac aagctgctgc ggctgccctc acaatgggag ctgccttcat agtgactggg 18180 actgtcaatc aagttgctaa acagtccggg acatgtgaca acgtgagaaa acaactcagt 18240 caagccacct acagtgacat ttgcatggct ccagcagctg atatgtttga ggaaggtgtg 18300 aaactccaag ttctcaagaa gggaacaatg ttcccatcaa gagctaacaa attgtacgaa 18360 ctcttttgca agtacgactc tttcgattca atgcctcctg ctgaacttga aaggatcgag 18420 aagaggatct tcaagagggc acttcaagag gtctgggaag agaccaagga cttttacatc 18480 aatggtttga agaatccaga gaagatacag agagcggaac atgaccccaa actcaagatg 18540 tctttgtgtt tcagatggta tcttggtctt gcatcacgtt gggccaacat gggtgctcct 18600 gatcgtgtca tggactatca agtctggtgt ggtccagcca ttggagcctt caatgacttc 18660 atcaaaggaa catatcttga tcctgctgtc tctaatgagt acccatgtgt tgtccagatc 18720 aaccttcaga ttttgcgtgg tgcatgttac ctgaggcgtc ttaatgctct gagaaatgat 18780 ccgaggattg atcttgagac tgaagatgct gcctttgtct atgagccaac gaatgcgctt 18840 tgagtagtta gcttaatcac ttaggtcacc cagtccaagc ccggcctagc acgatgatca 18900 tgagtgttgc tattgatgta tgttaatact atacacagtc atcgtgtgtg ttttataaaa 18960 ctgtaagctt aggatgtttg aggctaatgt aattagcact acaccgtaat aaatgagagg 19020 tttactttat gttttaataa tgttcttacc cttgctgctc tctaaactgt aatggcgtac 19080 ggttagctaa actaaagtaa aaatcgattc gcttgagtaa aattagtgac cgggaaagat 19140 catcgactac tactgctcag agatgtaatc ggtaattaag acttcaagct caataattaa 19200 ttaggagtaa atctggcttc gaatttttaa attgtcaact tccaaagaag gattggcaag 19260 actcaatctc aattgcccaa caaacatatg gctctttaca aatttggaat ctctttttga 19320 tggcaagaac gtcgtaattc ctctgtcgat ctcatgtaat taccaaactc atctacttat 19380 tactttcttt atatgcactt actgatgcgg ccgcttaatt aatcacgagt ggatgactgt 19440 cacgactgac tcatgactga ctactagtag cacatctgta ctcactcagg tctagtggtc 19500 aggtcagatt aattaagcgg ccgcgaattc ggcgcgccaa ccttggactc ccatgttggc 19560 aaaggcaacc aaacaaacaa tgaatgatcc gctcctgcat atggggcggt ttgagtattt 19620 caactgccat ttgggctgaa ttgaagacat gctcctgtca gaaattccgt gatcttactc 19680 aatattcagt aatctcggcc aatatcctaa atgtgcgtgg ctttatctgt ctttgtattg 19740 tttcatcaat tcatgtaacg tttgcttttc ttatgaattt tcaaataaat tatcgatagt 19800 actacgaata tttcgtatcg ctgatcttct caatcacaat gatgcgtagt gacccgacaa 19860 ataatttaag cgtccttaat accaatccta aaataattga ggcaaataaa atttttttgt 19920 aatttttatg atagcagatc gattctccag caagcctgca acaaaatatt gtgtatttct 19980 aaatagattt tgatactcga ggtcgacaca actttgtata ataaagttgt ctagacctag 20040 tagcacatct gtactcactc aggtctagtg gtcaggtcag attaattaag cggccgcgaa 20100 ttcgttggta gggtgctagg aaacttgttt ttggggtttt gtataagggt tgaaacatcc 20160 ctgaagtgtc tcattttatt ttatttattc tttgctgata aaaaaataaa ataaaagaag 20220 ctaagcacac ggtcaaccat tgctctactg ctaaaagggt tatgtgtagt gttttactgc 20280 ataaattatg cagcaaacaa gacaactcaa attaaaaaat ttcctttgct tgtttttttg 20340 ttgtctctga cttgactttc ttgtggaagt tggttgtata aggattggga caccattgtc 20400 cttcttaatt taattttatt ctttgctgat aaaaaaaaaa aatttcatat agtgttaaat 20460 aataatttgt taaataacca aaaagtcaaa tatgtttact ctcgtttaaa taattgagat 20520 tcgttccagc aaggctaaac gattgtatag atttatgaca atatttactt ttttatagat 20580 aaatgttata ttataataaa tttatataca tatattatat gttatttatt atttattatt 20640 attttaaatc cttcaatatt ttatcaaacc aactcataat ttttttttta tctgtaagaa 20700 gcaataaaat taaatagacc cactttaagg atgatccaac ctttatacag agtaagagag 20760 ttcaaatagt accttttcat atacatatca actaaaatat tagaaatatc atggatcaaa 20820 ccttataaag acattaaata agtggataag tataatatat aaatgggtag tatataatat 20880 ataaatggat acaaacttct ctctttataa ttgttatgtc tccttaacat cctaatataa 20940 tacataagtg ggtaatatat aatatataaa tggagacaaa cttcttccat tataattgtt 21000 atgtcttctt aacacttatg tctcgttcac aatgctaaag ttagaattgt ttagaaagtc 21060 ttatagtaca catttgtttt tgtactattt gaagcattcc ataagccgtc acgattcaga 21120 tgatttataa taataagagg aaatttatca tagaacaata aggtgcatag atagagtgtt 21180 aatatatcat aacatccttt gtttattcat agaagaagtg agatggagct cagttattat 21240 actgttacat ggtcggatac aatattccat gctctccatg agctcttaca cctacatgca 21300 ttttagttca tactggtgac ctaagtgatt aagctaacta ctcacagctt ctcagcaggg 21360 acatatgtgt catcaatgct cgccacaagt gcagctgcac catctgaaag ccttgcattt 21420 ctcagtattt caagacgcct caagaagcac gctcctctaa gaatctgttt gttgatctgc 21480 acaacacacg ggtactcatt tgccactgcc ggatcaaggt aagttccttt gatgaaatca 21540 ttgaatgaac caatggcagg accacaccaa acttggtagt ccatgactct gtcagaagct 21600 ccagtgttgg cccaccttga agccagggac agataccatc tgaaacacag tgacatcttc 21660 agtttggggt ccctctcagc cctctgtatc ttctctgggt tgtggagacg gttgatgtag 21720 aagttcttgg tctcatccca gacctcttcc aatgcacgac tgaagattct tttctcaacc 21780 ctcgcaagct ctgctggagg catggactca aaagaatcat acttgcagaa caactcatag 21840 agtttgttgg ctcttgaagg gaacattgtt cctttcttca aaacttgaag tttcacacct 21900 tcctcaaaca tgtcagctgc cggtgccatg caaacatctg agtaagttgc tttcgcaagc 21960 tgtttcctca cattgtcaca cgtcccagat tgtttggcaa cttggttcac tgttccagtg 22020 acaatgaaag aggcacccat gttgaaagtt gccagcgcag cttggggaca cccaatgcca 22080 cccccagcac caacacgaac cctcaggttt gctgggtatc cacactctct gtgaagccta 22140 tccctcaggt tgataatcag aggcagaatg acatggattg gacggttgtc agtgtgtcca 22200 ccagaatcag cttcaaccgc aatgtcatca gccacaggaa cacgtctggc caactcagct 22260 tgttcttgat tgatctcccc agaagcaatg agcttctgca aaagatgctc aggtgcagga 22320 cgcatgaaca tttcagccag ctcagttctg gagactttcc caatgattct gttccttatg 22380 ttgactgacc catctgcatt cctcgtcaac ccagccgctc tgtatctgac aacttgcggt 22440 gtcaacgtca tgaaggctga agcctcaaca aaagtgacac ccttttccaa gaagagatca 22500 acatttccct tctcaaggtt gctgtcaaaa ggagaatgga tgaggttgac agcatatggt 22560 ccattgggca gggcagcttg aatcttttca atggactcac gcacaacttg cattggaagg 22620 cctccagcac caaatgacgc aaggattcct tgccttccag ctgcgatcac caagtccgca 22680 gaggcaattc ccttggccat tgcaccggtg tacagaggcg cagaaacacc ataagttttc 22740 atgaaagctc tgctgccaag atcagcaatg ttgcacggag ggacaatgac actggcatca 22800 gatggcgcag tctcaacaag gttgccagaa gcacttgcag agctgagcgc aagcatggag 22860 tcaagatcaa gcagggcact acgaagtgca tcagcactgg caacagcttt tgtctccgca 22920 gctggggcag ctttcttctg aactggagct gaaggagcag gagacggaga tgaactgctg 22980 gaagaggaac tgctggaaga gctggagctg gaaggcgcat tgtccttgtt caagatcgga 23040 tctctggtct catcttgctc atcaatgttg agacgcgcat agaatttcgg agccacaggc 23100 ttcatgatcc ttgaagagat ggcagcttca atcgcagggt ccgcaggtgg gaaggatgca 23160 aggtctgctg aggagatggg gtctgccttg ctgttgaatc tgccattcaa ctgaatcttt 23220 ctcacaaact tgtttttctt gggtttctca cctttacaga gcgcagcata acaagcctca 23280 gcctctgcaa cgagcttgga gtggtagagc ggactgatgg tcactccagg aacaaggtga 23340 gccttcaatg aagccacgag cttgactatt gtcgtccaag catcctcgtt ctgcttgtct 23400 atggcaccag caaggtggtt tctctgtggt ccaagcgtgg ttctcactgc tgtgcttctg 23460 tgattgtttg gcccaacttc aacaaacaca tcatagttct gcttgtagat ggtttcaact 23520 atctgaggga agttggcttg tttctcatag agctgtccag catactcccc aaaggatgaa 23580 ggagcccatt catctttggc acccgtggct cttggcacaa gcctcttctg gtttatggta 23640 gtccagagat caagaccatc aaccacaggg aactcaaggt tggcgtggat ctttgcgatg 23700 tccttggtgt aaggtcccac ctctgggcaa tgcccacaca tgccttgagt gactggaagc 23760 gcagggatgt tgccaccaag acgagcaatc gcagctttgc atgcatcagg ctttccactg 23820 atgagagcag tgttggcatc attgatgatg gtcaacctca catactttga gtctggtgct 23880 atggctgctt ctatgtcttg tttcgttccc ctcactatgt aaccttgcca gaactcatct 23940 tttgggacag actgtggaat gccccaggct tctctgagcg cattgaactc aacagccaga 24000 gctttgttcc agacatctga ctctctcaaa tcctttgtca actgatcgct tatcaaaccg 24060 tttttcttgg agaaagcaaa gatcatggaa atctctccca aactgagtcc aaaggctgct 24120 ttgggcgtga tgttgagaac atctctcgcc aggtttgtga aggcaattga ggtgaggatt 24180 ccaagacgga acatttcaat catgtttctg tcaaactctt gctgttgtga ctcaagctca 24240 ctcttgaagc tggcccttgg catgacccat ctgtcacctt cagcccagag acggtttgtt 24300 ttctcattga tgacctcatg aagttcaggc caaatcctat gaatgtcttg tgtgatgcca 24360 tagtaagggc tccttccttc tccatacatg aaggcaactc tgtcactggc caaaggctca 24420 ggcgcatatc tgcttccagc aggggaactc caatcacgtc tcatcttgag acatctcgga 24480 atgccttttg ccgcaagttc cacctctctc tggagcttgg atggagtgga gaccaagctg 24540 agagcaaggg gcttctgact tccagatgaa gctgcatctt gggcaagggt ctcaccaagg 24600 agttcaagaa agatcctacg ggcttgcgcc ttcaattcag actctctggg tgcagcaccc 24660 gtgggctgca agaacctttc ccttatcttg tccaatcttc caagaatctc ttcatgggag 24720 tctgctctca acacaatgag cttgggtgct tcctcatcca aactgattct gttctctctc 24780 tcgtagtgtc cctctgcttc agaaagcagc acactgtagc aacttctggt ttcactgact 24840 ccggaaacag ccgcataacg tctctcacca gggttcttca accaagctct tgaggtctgg 24900 cacgcaaaga gtgtgctgtc ccagggtgct tcaggtgcag gttcatccca gtcatctcca 24960 ttgctgggca ggtaacggtt gtagatgcaa agcgcagcct taatcaaact ggcagcacca 25020 gaagcataac cagtgtcacc aacagtggcc ttgacacttc cagttgcaac attccttggg 25080 agcttgtcat cgtcacgaag aatcgtctga agtccgccaa tctcttcctc agcagtgagt 25140 tcctttggga gaacagacgg gtccttcaaa tgcctcgctg aatcagcaga aagctccaac 25200 atctcgatgt ctccaggctt gactctggct tgatccaatg cctctctcaa acatgcagat 25260 ggaacatttc caggaactat ggcatccatg caagcataga ttctgtcatc cttggtgcag 25320 cttgtctcac gcttgagcac aaatgcacca cagccctctc caacaaagta accatcagca 25380 gctgcatcaa agctggctct cggggtgtca gatgtagaca ccttgaaacg ccttgacttg 25440 acgtaaaggt tctcagcact cccacagaga tcaacacccg caaccacaac cccatcaacc 25500 tctcccgttt ccaagaggta tttgccaagt tcagcacatc tgtacacaga attgttgcct 25560 tctgtgattg tgaaggaagg tcccgtgaag ccccattgtg aactgaccct tgttgcaacc 25620 aagttgccta tgtagctggt gtaagaagtg cttgtcccac aatcattgat gtactgcatc 25680 atgtcattga gtttcttgct ggcttcaggc ctcactctct ccttgagagc aacccttgca 25740 cggtgacggt agagttcaag atcggtcccc aaccccacaa agacagccac atttccacct 25800 ttcttcatgc cagagtccaa tatggcccta tcaattgtgg tgacagccag aagctgttga 25860 ggcaagagca tatcttcagg agtcattgga gtcctcagcc tctggaaatc aacttcaaca 25920 tcttcaatgt aacagccatg cggtgttgct ttgactccac aaagatcaag gaagtccttg 25980 tctttcccca ggaatctcca tctcttttct gggagtggta tagcaccatg agcaccagtg 26040 tagatggccc tttcaaaagc atccaatcct ttcaaagcac caaaagtggc atccatccca 26100 gtgattgcta ttctcatgtt tgactcacca ccacaacgtg cagagagagc agaaatggag 26160 tcatggccag tgcatgcagc attgcttggg tcatgttcct caaagacagc atgagcattg 26220 gtcccaccaa atccaaacgc tgagagacca gcacgcttcg gttctccatt cgtttctggc 26280 cagggaatgg cctcaccaga gacaaccaga gggtccatct tcgtttcgtc atctattccc 26340 ggagtgggtg gaatgatccc gtgcttcatg gagagaagca ctttgcacat gccagcaaac 26400 ccagctgcca ccaatgtgtg tccaaagttc ccttttgtgg ttccaaaacg tgggactttg 26460 ccttcaaagc aagccttcac agcatcaatt tccacacgat caccttgtgg agtcccagtt 26520 gcatggcatt caacatactg aatcttgtgg ggatgcacat tgatacgtgt gtaggtgtcc 26580 atgaggcact ttttctctga cggcaaaagg ggtttaagtg gcagaccagt gccactgttg 26640 ctgacattgg ctcccaaaag tgttccatag atgtgatctc catctctgat tgcgtcatcc 26700 aaacgtttga ggaccatgat ggaacctcct tcaccagggg tcaatccttg agaatctttg 26760 tgcagcggca tggacacatt ctgacccgtt ccaacaggca tggcttggaa ggttgagaac 26820 ccagagagta tgaaaaacgg ctccggcagg catgttgcac cacaaagcat cacatcagct 26880 gcaccagaga ccaagtgatc ttgcgccagt ctgagcacat agagcgcagt ggcacaggca 26940 gcatcaacag agtagtggag agcgccaaga ttgagttcct cagcaacaaa ggaagcaggg 27000 tccatgaaga ttcttctgtc accagcctct ggtttgttgg actgttctct ttctgaccag 27060 tgagaggcat ctttgaaaac tctggcacca agtttcttct caacatggtt ctggtacaca 27120 ttcaagagtt caccttggag gttgtccatc gggaagctga ggcatccaga aactatgcca 27180 catcttgttg aatctttgac agatgtttca gccaaggctt gtttggcaag gttcaaaagg 27240 agttcatgct cattgtctat ttcgttctca tcaagagttc catatgtctc attgcagaag 27300 gtgtcagcat acttgctcct ttcagctttg tagtgttcag cacggtaatt tgaaccaaga 27360 cgtttgtcgc tgatcacttt gctctcaact tttccattca tgagaacttc ccagaactcg 27420 tctttcgttt tgcatccagc atactgaaca gccatgccca caacagctat cctcttctcg 27480 tcatgcatct catgagccgc actcacgttc cttgcagcca tttttggttg tggatcctag 27540 agtagtattg aatatgagtt gggttggggt attatagtag tagagtagta gtactctgga 27600 tggatggatg atgaaagaag tgagtgatat tagaggtatt tataggtatt atataagaga 27660 gaaggtggtt ggaacatgca tggagatttg ggcatgggat gacacgcata tgcaggttga 27720 cgtgtgttga agtgaagaaa ttgaggtggc ggaagagaat gaatatatac aggtggttgt 27780 ggtgatgatg aagaaaaagg caatgtgttt gtgtgtgggt tgagatgggt gagccattta 27840 aagtgcatgt taagcacgtg ttgctttgca tggcatttag acatacatgg acgcggcgat 27900 cttgatcagc cagtgactaa tttgagttgg ttgtgtgatt gcgttttgtc tctctgtttt 27960 gtcttttttc tttgttcttt gtctttttct tgcgcaagat ccatgcatga accaaaagac 28020 cacagagtgt catggcaacc cacagtaatt ccagttacgg acttacatac caagaaaagg 28080 taaaagcaat aagaaatata tgaaaattag tccaccataa atcttatagt ttatggttta 28140 gggtaacact ctaacactct actacattac ttatatttac tttaaactat ctataaaaca 28200 atttaaacat agtagaataa taaatcaata gtcacaaatt caacaattaa acttaaaatt 28260 aaaaaagtaa tattttaatt atatctaatt aattttttag aagtaatatt gagtatttga 28320 tatatgaaat cttgaatata gtaactatta ttaaaattac tttagaagat gtgtctcgca 28380 tgtaaaagca gatctaacta ctgtcactga ggccgtagac gagtacggac tgatctaact 28440 agtgctagcc tcgaggtcga cacccagctt tcttgtacaa agtggttgcg gccgcttaat 28500 taaatttaaa ttcaattaat gcaatcttga ttttcaacaa cgaaggtaat ggcgtaaaag 28560 aaaaaatgta tgttattgta ttgatctttc atgatgttga agcgtgccat aatatgatga 28620 tgtataatta aaatattaac tgtcgcattt tattgaaatg gcactgttat ttcaaccata 28680 tctttgattc tgttacatga cacgactgca agaagtaaat aatagacgcc gttgttaaag 28740 aattgctatc atatgtgcct aactagaggg aatttgagcg tcagacctaa tcaaatatta 28800 caaaatatct cactctgtcg ccagcaatgg tgtaatcagc gcagacaaat ggcgtaaaga 28860 tcgcggaaaa acctccccga gtggcatgat agctgcctct gtattgctga tttagtcagc 28920 cttatttgac ttaagggtgc cctcgttagt gacaaattgc tttcaaggag acagccatgc 28980 cccacacttt gttgaaaaac aaattgcctt tggggagacg gtaaagccag ttgctcttca 29040 ataaggaatg tcgaggaggc aatgtaaccg cctctggtag tacacttctc taatccaaaa 29100 atcaatttgt attcaagata ccgcaaaaaa cttatggttt aaaccctgca ggactagtcc 29160 agaaggtaat tatccaagat gtagcatcaa gaatccaatg tttacgggaa aaactatgga 29220 agtattatgt aagctcagca agaagcagat caatatgcgg cacatatgca acctatgttc 29280 aaaaatgaag aatgtacaga tacaagatcc tatactgcca gaatacgaag aagaatacgt 29340 agaaattgaa aaagaagaac caggcgaaga aaagaatctt gaagacgtaa gcactgacga 29400 caacaatgaa aagaagaaga taaggtcggt gattgtgaaa gagacataga ggacacatgt 29460 aaggtggaaa atgtaagggc ggaaagtaac cttatcacaa aggaatctta tcccccacta 29520 cttatccttt tatatttttc cgtgtcattt ttgcccttga gttttcctat ataaggaacc 29580 aagttcggca tttgtgaaaa caagaaaaaa tttggtgtaa gctattttct ttgaagtact 29640 gaggatacaa cttcagagaa atttgtaagt ttgtaggtac cagatctgga tcccaaacca 29700 tgtctccgga gaggagacca gttgagatta ggccagctac agcagctgat atggccgcgg 29760 tttgtgatat cgttaaccat tacattgaga cgtctacagt gaactttagg acagagccac 29820 aaacaccaca agagtggatt gatgatctag agaggttgca agatagatac ccttggttgg 29880 ttgctgaggt tgagggtgtt gtggctggta ttgcttacgc tgggccctgg aaggctagga 29940 acgcttacga ttggacagtt gagagtactg tttacgtgtc acataggcat caaaggttgg 30000 gcctaggatc tacattgtac acacatttgc ttaagtctat ggaggcgcaa ggttttaagt 30060 ctgtggttgc tgttataggc cttccaaacg atccatctgt taggttgcat gaggctttgg 30120 gatacacagc ccggggtaca ttgcgcgcag ctggatacaa gcatggtgga tggcatgatg 30180 ttggtttttg gcaaagggat tttgagttgc cagctcctcc aaggccagtt aggccagtta 30240 cccaaatctg agtagttagc ttaatcacct agagctcgat cggcggcaat agcttcttag 30300 cgccatcccg ggttgatcct atctgtgttg aaatagttgc ggtgggcaag gctctctttc 30360 agaaagacag gcggccaaag gaacccaagg tgaggtgggc tatggctctc agttccttgt 30420 ggaagcgctt ggtctaaggt gcagaggtgt tagcgggatg aagcaaaagt gtccgattgt 30480 aacaagatat gttgatccta cgtaaggata ttaaagtatg tattcatcac taatataatc 30540 agtgtattcc aatatgtact acgatttcca atgtctttat tgtcgccgta tgtaatcggc 30600 gtcacaaaat aatccccggt gactttcttt taatccagga tgaaataata tgttattata 30660 atttttgcga tttggtccgt tataggaatt gaagtgtgct tgaggtcggt cgccaccact 30720 cccatttcat aattttacat gtatttgaaa aataaaaatt tatggtattc aatttaaaca 30780 cgtatacttg taaagaatga tatcttgaaa gaaatatagt ttaaatattt attgataaaa 30840 taacaagtca ggtattatag tccaagcaaa aacataaatt tattgatgca agtttaaatt 30900 cagaaatatt tcaataactg attatatcag ctggtacatt gccgtagatg aaagactgag 30960 tgcgatatta tggtgtaata cataggaatt cgtttaaacg atctgcgtct aattttcggt 31020 ccaacttgca caggaaagac gtcgaccgcg gtagctcttg cccagcagac tgggcttcca 31080 gtcctttcgc tcgatcgggt ccaatgttgt cctcagctgt gaaccggaag cggacgacca 31140 acagtggaag aactgaaagg aacgagccgt ctataccttg atgatcggcc tctggtgaag 31200 ggtatcatcg cagccaagca agctcatgaa aggctgatgg gggaggtgta taattatgag 31260 gcccacggcg ggcttattct ttagggagga tctatctcgt tgctcaagtg catggcgcaa 31320 agcagttatt ggagtgcgga ttttcgttgg catattattc gccacgagtt agcagacgaa 31380 gagaccttca tgaacgtggc caaggccaga gttaagcaga tgttacgccc tgctgcaggc 31440 ctttctatta tccaatagtt ggttgatctt tggaaagagc ctcggctgag gcccatactg 31500 aaagagatcg atggatatcg atatgccatg ttgtttgcta gccagaacca gatcacatcc 31560 gatatgctat tgcagcttga cgcagatatg gaggataagt tgattcatgg gatcgctcag 31620 gagtagctca tccatgcacg ccgacaagaa cagaaattcc gtcgagttaa cgcagccgct 31680 tacgacggat tcgaaggtca tccattcgga atgtattagt ttgcaccagc tccgcgtcac 31740 acctgtcttc atttgaataa gatgttagca attgttttta gctttgtctt gttgtggcag 31800 ggcggcaagt gcttcagaca tcattctgtt ttcaaatttt atgctggaga acagcttctt 31860 aattcctttg gaaataatag actgcgtctt aaaattcaga tgtctggata tagatatgat 31920 tgtaaaataa cctatttaag tgtcatttag aacataagtt ttatgaatgt tcttccattt 31980 tcgtcatcga acgaataaga gtaaatacac cttttttaac attacaaata agttcttata 32040 cgttgtttat acaccgggaa tcatttccat tattttcgcg caaaagtcac ggatattcgt 32100 gaaagcgaca taaactgcga aatttgcggg gagtgtcttg agtttgcctc gaggctagcg 32160 catgcacata gacacacaca tcatctcatt gatgcttggt aataattgtc attagattgt 32220 ttttatgcat agatgcactc gaaatcagcc aattttagac aagtatcaaa cggatgtgac 32280 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgattt 32340 acaattgaat atatcctgcc ccagccagcc aacagctcga tttacaattg aatatatcct 32400 gccggccggc ccacgcgtgt cgaggaattc tgatctggcc cccatttgga cgtgaatgta 32460 gacacgtcga aataaagatt tccgaattag aataatttgt ttattgcttt cgcctataaa 32520 tacgacggat cgtaatttgt cgttttatca aaatgtactt tcattttata ataacgctgc 32580 ggacatctac atttttgaat tgaaaaaaaa ttggtaatta ctctttcttt ttctccatat 32640 tgaccatcat actcattgct gatccatgta gatttcccgg acatgaagcc atttacaatt 32700 gaatatatcc tgccgccgct gccgctttgc acccggtgga gcttgcatgt tggtttctac 32760 gcagaactga gccggttagg cagataattt ccattgagaa ctgagccatg tgcaccttcc 32820 ccccaacacg gtgagcgacg gggcaacgga gtgatccaca tgggactttt aaacatcatc 32880 cgtcggatgg cgttgcgaga gaagcagtcg atccgtgaga tcagccgacg caccgggcag 32940 gcgcgcaaca cgatcgcaaa gtatttgaac gcaggtacaa tcgagccgac gttcacgcgg 33000 aacgaccaag caagcttggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc 33060 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc 33120 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt 33180 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg 33240 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg 33300 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc 33360 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat 33420 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct 33480 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc 33540 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag 33600 gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc 33660 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt 33720 cggaaagggt cgacggatct tttccgctgc ataaccctgc ttcggggtca ttatagcgat 33780 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt 33840 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc 33900 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa 33960 tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc 34020 tgatgaaacc aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga 34080 cgaacgaaga gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct 34140 gctggccgtc ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga 34200 gctggcccgc atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac 34260 cgacgacccg cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat 34320 cgaagagaag caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga 34380 gccatgactt ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc 34440 ccatgcgctc catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc 34500 ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata 34560 aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg 34620 ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga 34680 aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg 34740 caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc 34800 agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg 34860 cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga 34920 agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg 34980 aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc 35040 agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc 35100 gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc 35160 cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct 35220 acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt 35280 acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg 35340 acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc 35400 tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc 35460 tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga 35520 tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga 35580 agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg 35640 agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg 35700 tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg 35760 tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt 35820 cagttccggc tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca 35880 ctgctcgacg cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact 35940 gccgataaac agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg 36000 agcggccgac gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga 36060 gatgttcggg tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg 36120 gttgcgagat gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt 36180 cttcaaacag gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt 36240 ggagcccgaa cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg 36300 tttattgctc gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt 36360 catcctcggc gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg 36420 cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat 36480 ctctgccgct ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg 36540 aactgcgggc gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt 36600 cgcagcgggc ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg 36660 gcaacctccc gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct 36720 gctcgttcca gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt 36780 tctcggcctg gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg 36840 ggggatctcg cgactcgaac ctacagttgt ttccttactg ggctttctca gcccccgagc 36900 gcttagtggg aatttgtacc ccttatcgaa ccgggagcac aggatgacgc ctaacaattc 36960 attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat catgagggaa 37020 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat 37080 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag 37140 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg 37200 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc 37260 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct 37320 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag 37380 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt 37440 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt 37500 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag 37560 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg 37620 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc 37680 atacttgaag ctaggcaggc ttatcttgga caagaagatc gcttggcctc gcgcgcagat 37740 cagttggaag aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa 37800 tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga 37860 gagctgggga agactatgcg cgatctgttg aaggtggttc taagcctcgt cttgcgatgg 37920 catttcgatc cattcccatt ccgcgctcaa gatggcttcc cctcggcagt tcatcagggc 37980 taaatcaatc tagccgactt gtccggtgaa atgggctgca ctccaacaga aacaatcaaa 38040 caaacataca cagcgactta ttcacacgag ctca 38074 <210> 65 <211> 36718 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB108208 <400> 65 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcatc agtaagtgca tataaagaaa gtaataagta 300 gatgagtttg gtaattacat gagatcgaca gaggaattac gacgttcttg ccatcaaaaa 360 gagattccaa atttgtaaag agccatatgt ttgttgggca attgagattg agtcttgcca 420 atccttcttt ggaagttgac aatttaaaaa ttcgaagcca gatttactcc taattaatta 480 ttgagcttga agtcttaatt accgattaca tctctgagca gtagtagtcg atgatctttc 540 ccggtcacta attttactca agcgaatcga tttttacttt agtttagcta accgtacgcc 600 attacagttt agagagcagc aagggtaaga acattattaa aacataaagt aaacctctca 660 tttattacgg tgtagtgcta attacattag cctcaaacat cctaagctta cagttttata 720 aaacacacac gatgactgtg tatagtatta acatacatca atagcaacac tcatgatcat 780 cgtgctaggc cgggcttgga ctgggtgacc taagtgatta agctaactac tcagaacgca 840 agtgagtctg tcgcaatgac agaggcttga acatcaatga agagatcacc ttgttcgttg 900 tggaactgaa gtgcatgttt gtgctggcta tgtccaccac tctgattgga tctgagggtg 960 atgtagaagg gtttgtcttg tgggaccggt ctgtgttgga ctatcctctg aatggagttc 1020 gggagtgcag cttgtcccag cgttctcctc acccagacca acattgcttg aaaggcaaga 1080 tcattgacaa agggatcatg tgcatcagtg tcagttgcaa actctcctct ggctgcatct 1140 gatccaggaa cagcagaaca cttggcaaca agctggctct tggtgcagct gagaacgtca 1200 tcaatgcctc tgaacgcagg accatgaaag agagtcttgc catcatagac tgacccttgc 1260 aaagcaggat ctgcatcaag gctcggaggc tgcatggttg cattggcagg tggagcacct 1320 tggttggaca acactatgac agctctgtag gctggaacaa gttttccact tgagaacgtc 1380 ttgagcgtcg cttggacatt caccctccca ctcggagcag tggacggtgt gagggtcact 1440 tcacagttga cgtctccatc aacagtgaca cctttgaaga gctgtgcgtc atctatggcc 1500 cagagagagt agccagggaa gagtccaagg caggtctcag caaggctccc aatagcaagg 1560 gtcattggca acacccttct gccttgtatg acatgatcct ccaagaaggg attggacttg 1620 gcactgatct ttctatgcag ggtgatcgtg tcacttccaa ctttcttgga tggtgtcctc 1680 cagtttccaa caaggatctc agcaggactt gatcccaaca caatcctcgc aactgtatca 1740 gcacctcctt cacgaggaat gatctgaact cccatttctt gaaactgttt cttgagttga 1800 ggtgtcacca tcccaccatc ccagggacca aagcagatgg acttgacact gacatccttc 1860 gcaagttcca aacccatctt gttgagagct tcgtttgcca tcgcatagtc agactgacca 1920 acattgccgt ggaaaccagc aagggagctg aagagaacca tgtgtttgag gttggcacga 1980 tcaactgcag ccagaagatt ctccaaccca gtgactttgg tcccaaagac agcatcaaac 2040 tcgtcaggga gtttcttctc tatgagacga tcacggagca caccagaagc gtgaacgatt 2100 ccactaactc tggcaccaag ctgggactct gcatccctaa ctgctttggc aacatctgca 2160 gcagagttca catcacaaga gctgtagatc gctttgccac ccagagcttc aatggcagca 2220 atggagcttc tcacttcacg agcaccaaga acagatccca caagtttcgt gacagccctt 2280 ggagtgggtt tgggtccttc accagcactg aaggccctct tcaactcttg ggtggcagct 2340 ttctgcacag ccttctcatc agtgatgcca gcacaccatg ctggttcaga cgcagaaact 2400 ttgctacgac ccaacagaat gtacttgcct ccagcaatct gacgcgtgat ctcacggatg 2460 caaagaggtg tgatcccacg agcacctcca gaaaccagaa gcacatcgtc tttcgctatc 2520 tgtctctgag ggttcccagt ctccaacttg gcagctctga ttgtgcatct ttgctggttt 2580 gctccaatcc caacctctct gattctgatg tcagcacacg ccatctccct aactatggca 2640 actgcagcat cttctgggtg catcccttga gcaatgtcca caccacggct gaacacatca 2700 gattctgacc actccaaccc tattgtcttg cagagtccaa agatcgctcc acgctgtgct 2760 ctcttgagtg cgtcagaagt gccttgagag gtgaatccaa gtctgccgtc aagcctagcc 2820 acaccgatga aggcaggacg acctccagcc acagcagtgc agagagaggc tttggcaaac 2880 ttcgcacaca tgagtgtgaa acccagtatc tcagctggtt caaacctttc tgcttgctgg 2940 cttatgaagc ctcccaacgc tccaaatctt tgctcagcct ctttgatggc cttctcagct 3000 gattctgctg aaaggtcctt agccagaaca tgacgaatgg cagctgcacc agctctctga 3060 gccaactgca atccctcaaa ggtcaagaca actgcacagg ctcccaacac cctcacaaga 3120 gcaagtgtca actctgagcc atcgtcaacg acaagaactg gacggttctc cggtctttcc 3180 agaatcagtt catctggcct tctgatgtca accacctttg cgtgcatcag tgacaaatcg 3240 tcacagtcag caccatgaac tgaatcaatc ttcacagctg gtgcagcacc ggcacttgca 3300 ggagcggctg cagccggagc aggggctgat ccaccagcta tctcagcctt catggcatcg 3360 accacttctc caaccgttct ggtccttgac aaagcatcga catctttggc ctcaacgttc 3420 agcatggctt gaacctcaga aaggatttcc acacgcttga tgctatcaat cccaagctct 3480 gtttccaact ccatgtctga ctctatcatg tcagtctcgt atccagtctt tgcagccaaa 3540 acctccatca caacagtttc agctttttca aggagttcgt tgctgacagc tggtgccgcg 3600 gcagcgggag caggagcggc tgcagctggc gcaggagcag aacccccagc tatctcagcc 3660 ttcatggcat caaccacctc tcccaccgtc cttgttctgg aaagagcatc aacatctttg 3720 gcctccacat tcaacatcgc ttgaacctca gagaggattt caacacgttt gatggagtca 3780 attccaagct cggtctccag ttccatgtca gattcaatca tgtcggtctc atagccagtt 3840 ttcgcagcaa ggacttccat cacaactgtc tcagcctttt ccaggagttc tgaggagacg 3900 gccggtgcgg ctgcagctgg agccggtgcc gcggctgcgg gtgcaggagc ggctgcagct 3960 ggcgcaggcg cggaacttcc agcgatctcc gccttcatcg catcaacgac ctcaccaact 4020 gttctggtcc ttgagagggc atccacatcc ttagcctcca cattgagcat tgcttggact 4080 tcactgagga tctcaacacg cttgatgctg tcaatgccca attccgtctc aagttccatg 4140 tcagactcaa tcatgtcagt ctcatagccg gtttttgcag ccagcacttc catgacaact 4200 gtttcagcct tctcaagcaa ttcgttgctg acagctggtg ccgcggctgc gggtgcaggt 4260 gcggcagctg ccggcgcagg agcagagcct ccagcaatct cagccttcat cgcatcgaca 4320 acttccccca cagttcttgt cctactgagt gcatcaacat ctttagcctc aacgttgagc 4380 atggcttgca cctcagagag gatctccact ctcttgatgg aatctattcc aagttcggtc 4440 tccaattcca tgtctgactc aatcatgtcc gtttcgtaac cagtttttgc agcaaggact 4500 tccatcacaa ctgtttcggc tttctccagc aactcattgc tgacggcagg agcggctgca 4560 gctggagcag gtgccgcggc agcaggtgca ggtgcggctg cagctggcgc actagcagag 4620 cttccggcaa tttcagcctt catggcgtcc acgacctcac cgactgttct ggttcttgaa 4680 agcgcatcga catcttttgc ctccacgttc aacatagctt ggacctcaga caagatttca 4740 acacgtttga tgctatctat tcccaactcg gtttcaagct ccatgtcact ttcaatcatg 4800 tcggtctcgt aaccagtctt agctgcgagc acctccatca caaccgtctc ggccttttcc 4860 aggagttcgg atgaaacagc tggcgcgggc gcggccggtg ctggtgcagc tggagctgga 4920 gctgcggctg ccggtgcagg tgccgcggca gctggcgcag gcgcgctccc tccagcaatc 4980 tccgccttca tggcatccac aacctcaccc actgtccttg ttcttgaaag tgcatcaaca 5040 tccttagctt caacattgag catggcttgg acttcactca ggatctccac tcttttgatg 5100 gaatcgatcc caagctctgt ctccaattcc atatcagatt caatcatgtc ggtctcatag 5160 ccagtctttg cagccaaaac ctccatcaca accgtctcag ccttctccaa gagttctgaa 5220 gaaacggcag gtgcaggtgc agctggagca ggtgccgcgg ctgcgggtgc aggagcggct 5280 gcagctggcg cgggcgcaga ccctccagca atttcagcct tcatggcatc cacaacctca 5340 ccaacggtcc tcgttctgga cagagcatca acatccttcg cttccacgtt cagcatagct 5400 tggacctcac tgaggatttc aacacgtttg atgctgtcta tcccaagttc tgtctccaat 5460 tccatgtcag actcaatcat gtcagtctca tagccggttt tagctgccaa gacctccatc 5520 acgacggtct cagctttttc aaggagttcg tttgagacgg ccggagcggc tgcagctgga 5580 gctggagccg cggcagcggg accaggagcg gcagcggccg gcgcaggagc ggagcctcca 5640 gctatctcag ccttcatggc attgacaacc tcaccgacag ttctggtcct tgagagagca 5700 tccacatcct tagcttccac attgagcatg gcttggactt cactgaggat ttccacacgc 5760 ttgatggaat caattccaag ctccgtctca agctccatgt ctgattctat catgtcagtt 5820 tcgtagccag ttttggcagc aaggacttcc atcacaacag tttctgcctt ttcgagcagc 5880 tcattagaaa cagctggtgc agctgcagct ggagccgctt tagccggtgc aggtgcggca 5940 gctgccggcg ccggagcgga agaaccagcg atttctgcct tcatagcgtt gacaacttca 6000 cccactgttc tcgtcctaga gagagcatca acatctttcg cctcaacgtt aagcatagct 6060 tggacttcag aaagaatttc aaccctcttg atgctgtcaa ttcccaactc agtctcaagc 6120 tccatgtccg cctcaatcat gtctgtttcg tatccagtct tggcagcgag aacctccatc 6180 acaactgtct cagctttctc aagcaactcg ttggacacgg cgggagcagg agcacttgca 6240 acgggagcgg caggggcagc ggctttaaca ggcgccggag cagtctgttg ctgttgctgt 6300 tgctgaaggg aagaagcatc aacacttcca taaccatcca gctcagccag catggacttg 6360 aggatggcac gatgtttctc cacaacagca gtgtcaactg caggcttggc tgaagctgca 6420 gcagtcttgg cttcctcctt ggcagcagca agtttggagt tggcttctgc agctgcacgt 6480 ttggcgtcgt caagctgcct ctgagcgtct tgaagctcct tctgaagcct ttcagcctcc 6540 ctcttggcag cttcgtcaac cacaggttca ggagccttga tgagtggtgc agcacccttg 6600 aggtatgtga cacatcttcc gtcgttcatg gcagcatccc tcactttctt ggtcttgtca 6660 ctgacatagg tggcagctga aagtctgagt gtggttctct ttttcttgat ggcttgcatc 6720 ctcgtggcat caggagcatc ccacttgtca aacccttgaa gattgacacc agccacaacc 6780 agctgaactg cagcatccct caactgtatg tcagagtccg tgccagaagc tgggttcaca 6840 gacactgtga caacagacgg gtcatctttg agggtttcag aaacaagttt gctgagaact 6900 tgtttcggtc caaactcaac aaagattctc gcaccagctt ggtgcatgtt cctcacttgg 6960 gtcaggaact tcacagatga ggtcatgtgt tgagtgagca tctctctggc atccgtcgga 7020 taggtctctc cagaaacatt gctgaagagc tttgtttctg ctttgggtgt acggaaggaa 7080 accttggata tgacatcttt gaaagctgag ctggcatttt ccatctgtgg agaatggaag 7140 gcagactcac aagcaagagg cacaactctg aacccttcct tctgcaatct ggcactttca 7200 gcttgaatcc cttcaacaga cccagtaatg acagtctgag acggagagtt tgagtttcca 7260 agccagacgt tggcagcttg aaccttgatg ttctctgcgt tgggtcctat gacagctgcc 7320 atgcaacctt tgggtgtggc aggtgcatct ttgcctccca tgatccttgc ccttctgcaa 7380 acaagttcaa acaactcatc tctgtcaaca caaccagctg catagagcgc agcaaactca 7440 cccaagctgt gtccagctgc aaagtctgga gtgaatccag cttccttgaa aatctcaaag 7500 gcaccaagag cacatgccag ggttgacggc tggctgtatg ctgtgagtga gattttcttg 7560 tgatcttgtt ctggctcacg ttcgtacggt ttccttgggt acaggacttg agagactctt 7620 tcaaagtctt tgtcacttcc agcaaccttg gactgtgcag cgtccattgc agcaatggac 7680 tgtctgaact gaggccagtt catcgcaact tcactgaaca tgtgtgtgta ctgagcacct 7740 tgtccagaga agagtgcagc aacagcacca ttggtggcaa tgcctttggc acggaaagaa 7800 actccttctc tcggaagcct ccacgcttct ttggtcacat ctttggcaaa ctgagcacat 7860 atagcacgaa gcgtggagca cgcatcctcc gcatctttga ccaagaatcc cagtctcgca 7920 ttggtcgcag gtatgctccc agggaacttg aactgttctc caaacttgac acacttgatg 7980 taggcagtat tcttgactgt ctcattctcc ttgatcgcag cttcaaactc tttcagctga 8040 gcttcacaaa ggctctgcaa cgcagcagga gttgcagcca tcatcaacac cggttgtgga 8100 cgtttgttga gtctgtacgc tgtcgtgtgc tctggttcag cctcttccaa cactgcatgg 8160 tagttggcac ctccaaagcc aaagctggat attccagcac gtctcggaac accaggtgga 8220 gggaaccagg gtctgttcat cgtgttgatg tagagggatg actcattgat gggagtgttg 8280 tcatagagat tgggtggatt gtccacattg atcgttccag gcaaagtctt gtgtttgaga 8340 gccatgatga ccttgatcat tccagcaaga ccagcaacag ccttcaaatg accaatactg 8400 gacttgatac ttccaactgc aactttctct gtgttccctt caccatacgc tttgtcaaac 8460 aagttcctca acgcagtcaa ctcaatccta tctccaactg gagtgccagt cccatgaccc 8520 tcaaccaaag tgacagtggc agggtcaacg caggcacgat tgtatgcacg tctcaaggct 8580 tcctcttgac cactgattgt gggagtgtag atgccagctg ctttgccatc acttgagctt 8640 gcacaaccac gaatcacagc atgaatctca tcaccatccc tcactgcatc tgcataacgt 8700 ttgagcacca acatggctga tccctcacca atgagcatcc ctttcgtttt ctcatcatac 8760 gctctcacac ttgggtccgt gctgaacaca ggtgtctttg agaaagccat gtacatccca 8820 atggagttgt ctgtgcaggt tgcaccagtg accatcatgt cgcagtcacc atagagcagt 8880 tcatcaatcg caaccttgac tgctatcaag gagcttgcac atgcagcatc cacaacgcag 8940 ttcatgccat ccaagttgaa tgtgttggtg catctgccag cagtgacatt cccaaggaat 9000 ccagggaaag agtcaagtct ccattctggg aagttcgctt tgtacttctc cacagccact 9060 ttgacatctt cctctggcat ccccatcttt ctcaagactt tctccacaac cacatagttg 9120 agtctgctgt agaactcatg tgaactcttc tgtccacctc caatgccaag cacgcatcct 9180 atgtttttct tctcctttcc aagtgcatca atcccagcat cttgaagtgc ttctttgacc 9240 ttgagaagtg agatggtctg attcgcatca gaatcttcca tctggaacat gttgagacca 9300 aactcacgcg catcaaagtc atactcagga atgaaacctc ctctcttgca gtagattttg 9360 tcctttgtgg tcttgactgg gtcaaagtac gcagtcacat caacacgatc ctcaggaaga 9420 tcagacaggc aatcaatgcc agcccttatg gtctcccatg actctctcac cgtggttcca 9480 caaggaagta tggcgctcat gcctatgatc gcaatcctcg tgtccatctc acctcctttc 9540 tgctcttgca gcctcgctgc catttttggt tgtggatccg ttttgctatt tgtgtatgtt 9600 ttcttgtttt ggtttgagtt taaccatcat tctcttctct ctatttatag agagaagagg 9660 ttggcacgca gcactgcgac atgagagaaa gacacgggtg aagaggcggg agctgaggtg 9720 gcagcatgca tgcatgcatg gccagtcgtg tcatagtgag aagaggagaa agctgaggtg 9780 gcaacgtgta tggccagtgg tgtcatagtg tgaaagctga cgtggcagca tgcatggtga 9840 gtgtgaaaaa ggcgccacag tgcagatgtt actgaacaaa agcggtgtgc catgcaactt 9900 ttgcccatcg tcgtctttct catccaagtt cctctttata atatctttta ccaaatcata 9960 ttttaatata aattcatttt atttttaaat aaagtaatat tatatccata aatattaact 10020 ctcatttaac acattttcaa atttaacact tctgtaaata aatttattaa caaaaacttg 10080 taaatatgtt caaataaatc cataaatatt aactttcatt ttattaacaa aaaaatcttt 10140 actataagct gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctaggat 10260 ctgcttttac atgcgagaca catcttctaa agtaatttta ataatagtta ctatattcaa 10320 gatttcatat atcaaatact caatattact tctaaaaaat taattagata taattaaaat 10380 attacttttt taattttaag tttaattgtt gaatttgtga ctattgattt attattctac 10440 tatgtttaaa ttgttttata gatagtttaa agtaaatata agtaatgtag tagagtgtta 10500 gagtgttacc ctaaaccata aactataaga tttatggtgg actaattttc atatatttct 10560 tattgctttt accttttctt ggtatgtaag tccgtaactg gaattactgt gggttgccat 10620 gacactctgt ggtcttttgg ttcatgcatg gatcttgcgc aagaaaaaga caaagaacaa 10680 agaaaaaaga caaaacagag agacaaaacg caatcacaca accaactcaa attagtcact 10740 ggctgatcaa gatcgccgcg tccatgtatg tctaaatgcc atgcaaagca acacgtgctt 10800 aacatgcact ttaaatggct cacccatctc aacccacaca caaacacatt gcctttttct 10860 tcatcatcac cacaaccacc tgtatatatt cattctcttc cgccacctca atttcttcac 10920 ttcaacacac gtcaacctgc atatgcgtgt catcccatgc ccaaatctcc atgcatgttc 10980 caaccacctt ctctcttata taatacctat aaatacctct aatatcactc acttctttca 11040 tcatccatcc atccagagta ctactactct actactataa taccccaacc caactcatat 11100 tcaatactac tctaggatcc acaaccaaaa atgcttcagc acacttggct tccgaagcct 11160 cccaatctga ccctcttgtc agatgaggtt catctctgga ggattcctct tgaccagcct 11220 gagtcacaac ttcaagacct tgctgccacc ctgagcagtg atgaattggc gagggcaaac 11280 agattctact ttccagaaca cagaaggcgt ttcactgctg ggagaggcat cctcagatcc 11340 atcttgggtg gatacttggg agtggaaccg ggtcaagtca agtttgatta tgagtcccgt 11400 gggaaaccga tccttggtga cagatttgct gagagtggac tcctgttcaa cttgtctcac 11460 agccagaacc ttgccttgtg tgctgtcaac tacacgcgtc aaataggcat tgatcttgaa 11520 tatctgcgtc caacatctga cttggagtct cttgcaaaga ggttctttct cccaagagaa 11580 tatgaactct tgaggtcact ccctgatgag cagaaacaga agattttctt tcgttactgg 11640 acttgcaaag aggcttatct caaagcaacg ggagatggaa tagccaaact tgaagagatc 11700 gagatagcac tcaccccaac agaacctgcc aagctccaaa cagctcctgc gtggtctctg 11760 ttggagcttg tgccagatga caattgtgtt gcagctgtgg ctgttgcggg ttttggttgg 11820 cagcccaagt tctggcatta ctgagtagtt agcttaatca cttaggtcac cagtatgaac 11880 taaaatgcat gtaggtgtaa gagctcatgg agagcatgga atattgtatc cgaccatgta 11940 acagtataat aactgagctc catctcactt cttctatgaa taaacaaagg atgttatgat 12000 atattaacac tctatctatg caccttattg ttctatgata aatttcctct tattattata 12060 aatcatctga atcgtgacgg cttatggaat gcttcaaata gtacaaaaac aaatgtgtac 12120 tataagactt tctaaacaat tctaacttta gcattgtgaa cgagacataa gtgttaagaa 12180 gacataacaa ttataatgga agaagtttgt ctccatttat atattatata ttacccactt 12240 atgtattata ttaggatgtt aaggagacat aacaattata aagagagaag tttgtatcca 12300 tttatatatt atatactacc catttatata ttatacttat ccacttattt aatgtcttta 12360 taaggtttga tccatgatat ttctaatatt ttagttgata tgtatatgaa aaggtactat 12420 ttgaactctc ttactctgta taaaggttgg atcatcctta aagtgggtct atttaatttt 12480 attgcttctt acagataaaa aaaaaattat gagttggttt gataaaatat tgaaggattt 12540 aaaataataa taaataataa ataacatata atatatgtat ataaatttat tataatataa 12600 catttatcta taaaaaagta aatattgtca taaatctata caatcgttta gccttgctgg 12660 aacgaatctc aattatttaa acgagagtaa acatatttga ctttttggtt atttaacaaa 12720 ttattattta acactatatg aaattttttt tttttatcag caaagaataa aattaaatta 12780 agaaggacaa tggtgtccca atccttatac aaccaacttc cacaagaaag tcaagtcaga 12840 gacaacaaaa aaacaagcaa aggaaatttt ttaatttgag ttgtcttgtt tgctgcataa 12900 tttatgcagt aaaacactac acataaccct tttagcagta gagcaatggt tgaccgtgtg 12960 cttagcttct tttattttat ttttttatca gcaaagaata aataaaataa aatgagacac 13020 ttcagggatg tttcaaccct tatacaaaac cccaaaaaca agtttcctag caccctacca 13080 acgaattcgg tcacctcgag tatcaaaatc tatttagaaa tacacaatat tttgttgcag 13140 gcttgctgga gaatcgatct gctatcataa aaattacaaa aaaattttat ttgcctcaat 13200 tattttagga ttggtattaa ggacgcttaa attatttgtc gggtcactac gcatcattgt 13260 gattgagaag atcagcgata cgaaatattc gtagtactat cgataattta tttgaaaatt 13320 cataagaaaa gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg 13380 cacatttagg atattggccg agattactga atattgagta agatcacgga atttctgaca 13440 ggagcatgtc ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca 13500 ggagcggatc attcattgtt tgtttggttg cctttgccaa catgggagtc caaggttggc 13560 gcgccgaatt cgcggccgct taattaacta ctgtcactga ggccgtagac gagtacggac 13620 tgatctaact agtgctagcc tcgaggtcga ccaacttttc tatacaaagt tgtctagacc 13680 taggactagg cactgagtga ctcactgacc tgactgagtg actggcggcc gcatcagtaa 13740 gtgcatataa agaaagtaat aagtagatga gtttggtaat tacatgagat cgacagagga 13800 attacgacgt tcttgccatc aaaaagagat tccaaatttg taaagagcca tatgtttgtt 13860 gggcaattga gattgagtct tgccaatcct tctttggaag ttgacaattt aaaaattcga 13920 agccagattt actcctaatt aattattgag cttgaagtct taattaccga ttacatctct 13980 gagcagtagt agtcgatgat ctttcccggt cactaatttt actcaagcga atcgattttt 14040 actttagttt agctaaccgt acgccattac agtttagaga gcagcaaggg taagaacatt 14100 attaaaacat aaagtaaacc tctcatttat tacggtgtag tgctaattac attagcctca 14160 aacatcctaa gcttacagtt ttataaaaca cacacgatga ctgtgtatag tattaacata 14220 catcaatagc aacactcatg atcatcgtgc taggccgggc ttggactggg tgacctaagt 14280 gattaagcta actactcaaa gcgcattcgt tggctcatag acaaaggcag catcttcagt 14340 ctcaagatca atcctcggat catttctcag agcattaaga cgcctcaggt aacatgcacc 14400 acgcaaaatc tgaaggttga tctggacaac acatgggtac tcattagaga cagcaggatc 14460 aagatatgtt cctttgatga agtcattgaa ggctccaatg gctggaccac accagacttg 14520 atagtccatg acacgatcag gagcacccat gttggcccaa cgtgatgcaa gaccaagata 14580 ccatctgaaa cacaaagaca tcttgagttt ggggtcatgt tccgctctct gtatcttctc 14640 tggattcttc aaaccattga tgtaaaagtc cttggtctct tcccagacct cttgaagtgc 14700 cctcttgaag atcctcttct cgatcctttc aagttcagca ggaggcattg aatcgaaaga 14760 gtcgtacttg caaaagagtt cgtacaattt gttagctctt gatgggaaca ttgttccctt 14820 cttgagaact tggagtttca caccttcctc aaacatatca gctgctggag ccatgcaaat 14880 gtcactgtag gtggcttgac tgagttgttt tctcacgttg tcacatgtcc cggactgttt 14940 agcaacttga ttgacagtcc cagtcactat gaaggcagct cccattgtga gggcagccgc 15000 agcagcttgt gggcagccaa caccgcctcc agctccaact ctgactctaa ggtgcgcagg 15060 ataaccacac tctctgtgca gtctgttcct aaggttgatg atcaaaggca agatgacgtg 15120 gatgggacgg ttgtcagtgt gaccaccaga atcagcttca acagcgatgt cgtctgcgac 15180 aggaacacga cgggcaagtt cggcttgttc ttgtgttatc tcaccagaag ctatgagttt 15240 ctcaaggagg tgttccggag ccggtctgat gaacatttcg gcgagttctg ttctggacac 15300 ttttccaatg atacggtttc tgatgttgac actcccatca gcgttccttg agagtccggc 15360 tgcacggtaa cgaacaactt ggggagtgag ggtcatgaat gcagaagcct ccaccacagt 15420 aacgcccttt tcgaggaaga gatcaacatt ccccttttcc aagttgctgt caaaaggtga 15480 atgaatgagg ttcacagcat atggtccttg aggcagagcc gcttgtatct tctccaaagc 15540 tgccctcaca tggtgcattg gaagcccacc cgctccaaaa gaacccaata tcttcctctt 15600 cccagcagca atgacaaggt ccgcagacgc aattcccttg gccattgctc cagtgtacag 15660 aggtgctgac acattgtacg tttccatgaa ggacttgtca ccaagatcac ttatggagca 15720 tggtttgata ctgacaatcc ttgaagaagt tgcaggtttg gattcaaact gcaccaaatt 15780 ggagttttca acaccaagat acaatggatt gtccacttca agcaggacat ctctcaactg 15840 agtcggactg agtgcaactc cagttttccc agggtccgct tttgcgctgg gtggctgttg 15900 ttgaacatcc ggaatggctt gaggttttgc actgacaaca cgaactctga gttctttggc 15960 ttcatacaca cggagaccat caacccaaag tgctccatct gccactatgt caacgtagcc 16020 tccattttca ggagaggctg tgattgaaac tatgtgcacc tcacagtcca tcgcacggtt 16080 ctttggtgtc aattgaccac ggtacttcca cgccgttttt ccaggggcat gggcaaaggt 16140 cggattgctc acattgtatt ctcccggtat gttctggtcc accgcaaatg cctcaatgag 16200 ctggaacatt gactcaattc ccaaagatcc aggcatcaca ggatcaaacc aaaagtgaca 16260 agagaagaac cagtcttgtg ggttaacatc tttgtgtcca tgcgcatatc ctttcccatg 16320 cagcccagaa tcaggagcaa tgttgaggta atccaaatac tcacactgtt ctgaccttct 16380 gagaacttga gcattagttg catctggaag tttctcagtc agctgttctt tgcctttggc 16440 tgatgcgatt tcaactcttc tcacactcgt gttgttagtt ttgcaccaag gctccgtctt 16500 tttcccattg tcaagtccag cttgctgagc aaacacctct ggagtgaacc atccaaagga 16560 agtgcttcct ttgtagaaaa ccacgccatc aacagacaac tcaaacgtga atctgtgaat 16620 ccccatcttt cccaacattg catatccagt gcacttggtc acattcctta tggttttccc 16680 cctcacatcc acatctggcc tcaccatttc agcacttgca tcaaggttcc taaagagaat 16740 gtcatctttg tccatcgtca gtggtgcttt caacacactg gtgagaacac cggatgtctg 16800 aagtccgatt tccatgatga tggaataagg catgtgccca tcattgcaag aaccatcaaa 16860 gaaccacgca tcttgaggac agtcaaactc ccccaccatt gtgcctttgc tgggattgca 16920 atcaacattc aggaacttgc catgttccat gtttgtgact tcaacaactc tagtcacaag 16980 agccaaatca aaagcagggc ttctgctcgt atttgactta tcaaatcttg cgaactctgg 17040 tcccaagcag ttgctaactt ttccacacat gaactctgac atgttgtacc agttcagagg 17100 catttctcca gctttgcaat tgttgtcaag tgggttgcca gggaaaggga tgaagcaaat 17160 tgccctcgga gggtaagaac taggggagaa gaacggcgtc gggttgccag gaagttttga 17220 catgggatgc caggacattt ctttcggagc gagagcgtca cccttgagga tatgttcagg 17280 aagtgcaact ggctttggcg caagaacatc tgctgagaaa gcaggcttcg caggggcagc 17340 agaagcacta gcactggcac cagagctggc cttggacgct tccggtccca cagtggctgc 17400 accgttagca aaaacgggct gaactttgga ggggttcttg ttcgtggatc tcttctgcat 17460 cttgagagca atgcccttga agtccacgac gatctttttg ttaagatccc cctttccgta 17520 gtcgctgatt ctatcaaggc tgaaatcttg acccttttcg aaatcaacgt caatgatgtt 17580 gacgtcagca atggcgtacg gatcgttatc ctcgtcgaat cccatctctt tgatctccat 17640 gacgtagacc aactttccct tatgaggaga tatctgaccc ctacacctaa ctttgttcgg 17700 gtgtccgttg acgggtctga agtcaaatgg tccggtggta aggtgcaaac cgagccagat 17760 catgtacatc ttgagcattt gtgaacaacc gtcgctgaca agagatccag ccatgacttg 17820 atccttaaca aaatggcatg ggaagtacca atggtccctc tccaagattt tctcaccaac 17880 caactgaccc aaaccgtaca cacctccctt atggtcaatg ctggtgactc tatcaatcat 17940 gagcatctta cgtgcgcaga gtttgtagtt gatctccggc atgccattct ttgagccaaa 18000 aacggaagcc caatctttgt caaccaaggt ctgcatctcc ttttcgttga gttttgtttt 18060 gtgaaggcag ggagcgacag catagggaga aacatcttgc tttggaatct tagctctggc 18120 agccaaatcc cctcttgtga acaccacacc ctttcccgca tcaagctcct catttgtgaa 18180 gaagccagca cagccatctc tcatctcaat gagaagcctc ccattgacat agcagtcata 18240 ctcaaagaag aacatggaga tgccaccatc caaacgtttg gcaaaaccgg tcactctgat 18300 gtcatactca agggtctctc cctcatgggc aaccccatag aatgtcaacg tggtgttgag 18360 cagacggtaa actctgtcac cttggttctg gaaatcaatt cccatgtatg atatcagcat 18420 caaatcgcac tgtccactct caaccagaac tgcccaggga caatcacctc cctcagacaa 18480 ttcgccattc actggaagat catactcagt gaccattctg gcaccaactc tgtagttgtt 18540 gacctcggca tccatgagtg tgacccttgt cacaagcagg tattcacgag caggcagcct 18600 cacccttctg gggtacttgt ctatgacagc aaactccgga ccaaaaacct tagctatgtc 18660 accctctgca aactccagga gttcctcata gttgaacacc tcagttttgc cagaggtcag 18720 ttcctccttg gtcatctccc aacatggctt cttgttggtc ttgacacgaa gcgccatttt 18780 tggttgtgga tccgttttgc tatttgtgta tgttttcttg ttttggtttg agtttaacca 18840 tcattctctt ctctctattt atagagagaa gaggttggca cgcagcactg cgacatgaga 18900 gaaagacacg ggtgaagagg cgggagctga ggtggcagca tgcatgcatg catggccagt 18960 cgtgtcatag tgagaagagg agaaagctga ggtggcaacg tgtatggcca gtggtgtcat 19020 agtgtgaaag ctgacgtggc agcatgcatg gtgagtgtga aaaaggcgcc acagtgcaga 19080 tgttactgaa caaaagcggt gtgccatgca acttttgccc atcgtcgtct ttctcatcca 19140 agttcctctt tataatatct tttaccaaat catattttaa tataaattca ttttattttt 19200 aaataaagta atattatatc cataaatatt aactctcatt taacacattt tcaaatttaa 19260 cacttctgta aataaattta ttaacaaaaa cttgtaaata tgttcaaata aatccataaa 19320 tattaacttt cattttatta acaaaaaaat ctttactata agctgcggcc gcttaattaa 19380 tcacgagtgg atgactgtca cgactgactc atgactgact actagtgcta gcctcgaggt 19440 cgacacaact ttgtataata aagttgtcta gtctgctttt acatgcgaga cacatcttct 19500 aaagtaattt taataatagt tactatattc aagatttcat atatcaaata ctcaatatta 19560 cttctaaaaa attaattaga tataattaaa atattacttt tttaatttta agtttaattg 19620 ttgaatttgt gactattgat ttattattct actatgttta aattgtttta tagatagttt 19680 aaagtaaata taagtaatgt agtagagtgt tagagtgtta ccctaaacca taaactataa 19740 gatttatggt ggactaattt tcatatattt cttattgctt ttaccttttc ttggtatgta 19800 agtccgtaac tggaattact gtgggttgcc atgacactct gtggtctttt ggttcatgca 19860 tggatcttgc gcaagaaaaa gacaaagaac aaagaaaaaa gacaaaacag agagacaaaa 19920 cgcaatcaca caaccaactc aaattagtca ctggctgatc aagatcgccg cgtccatgta 19980 tgtctaaatg ccatgcaaag caacacgtgc ttaacatgca ctttaaatgg ctcacccatc 20040 tcaacccaca cacaaacaca ttgccttttt cttcatcatc accacaacca cctgtatata 20100 ttcattctct tccgccacct caatttcttc acttcaacac acgtcaacct gcatatgcgt 20160 gtcatcccat gcccaaatct ccatgcatgt tccaaccacc ttctctctta tataatacct 20220 ataaatacct ctaatatcac tcacttcttt catcatccat ccatccagag tactactact 20280 ctactactat aataccccaa cccaactcat attcaatact actctaggat ccacaaccaa 20340 aaatggctgc aaggaacgtg agtgcggctc atgagatgca tgacgagaag aggatagctg 20400 ttgtgggcat ggctgttcag tatgctggat gcaaaacgaa agacgagttc tgggaagttc 20460 tcatgaatgg aaaagttgag agcaaagtga tcagcgacaa acgtcttggt tcaaattacc 20520 gtgctgaaca ctacaaagct gaaaggagca agtatgctga caccttctgc aatgagacat 20580 atggaactct tgatgagaac gaaatagaca atgagcatga actccttttg aaccttgcca 20640 aacaagcctt ggctgaaaca tctgtcaaag attcaacaag atgtggcata gtttctggat 20700 gcctcagctt cccgatggac aacctccaag gtgaactctt gaatgtgtac cagaaccatg 20760 ttgagaagaa acttggtgcc agagttttca aagatgcctc tcactggtca gaaagagaac 20820 agtccaacaa accagaggct ggtgacagaa gaatcttcat ggaccctgct tcctttgttg 20880 ctgaggaact caatcttggc gctctccact actctgttga tgctgcctgt gccactgcgc 20940 tctatgtgct cagactggcg caagatcact tggtctctgg tgcagctgat gtgatgcttt 21000 gtggtgcaac atgcctgccg gagccgtttt tcatactctc tgggttctca accttccaag 21060 ccatgcctgt tggaacgggt cagaatgtgt ccatgccgct gcacaaagat tctcaaggat 21120 tgacccctgg tgaaggaggt tccatcatgg tcctcaaacg tttggatgac gcaatcagag 21180 atggagatca catctatgga acacttttgg gagccaatgt cagcaacagt ggcactggtc 21240 tgccacttaa accccttttg ccgtcagaga aaaagtgcct catggacacc tacacacgta 21300 tcaatgtgca tccccacaag attcagtatg ttgaatgcca tgcaactggg actccacaag 21360 gtgatcgtgt ggaaattgat gctgtgaagg cttgctttga aggcaaagtc ccacgttttg 21420 gaaccacaaa agggaacttt ggacacacat tggtggcagc tgggtttgct ggcatgtgca 21480 aagtgcttct ctccatgaag cacgggatca ttccacccac tccgggaata gatgacgaaa 21540 cgaagatgga ccctctggtt gtctctggtg aggccattcc ctggccagaa acgaatggag 21600 aaccgaagcg tgctggtctc tcagcgtttg gatttggtgg gaccaatgct catgctgtct 21660 ttgaggaaca tgacccaagc aatgctgcat gcactggcca tgactccatt tctgctctct 21720 ctgcacgttg tggtggtgag tcaaacatga gaatagcaat cactgggatg gatgccactt 21780 ttggtgcttt gaaaggattg gatgcttttg aaagggccat ctacactggt gctcatggtg 21840 ctataccact cccagaaaag agatggagat tcctggggaa agacaaggac ttccttgatc 21900 tttgtggagt caaagcaaca ccgcatggct gttacattga agatgttgaa gttgatttcc 21960 agaggctgag gactccaatg actcctgaag atatgctctt gcctcaacag cttctggctg 22020 tcaccacaat tgatagggcc atattggact ctggcatgaa gaaaggtgga aatgtggctg 22080 tctttgtggg gttggggacc gatcttgaac tctaccgtca ccgtgcaagg gttgctctca 22140 aggagagagt gaggcctgaa gccagcaaga aactcaatga catgatgcag tacatcaatg 22200 attgtgggac aagcacttct tacaccagct acataggcaa cttggttgca acaagggtca 22260 gttcacaatg gggcttcacg ggaccttcct tcacaatcac agaaggcaac aattctgtgt 22320 acagatgtgc tgaacttggc aaatacctct tggaaacggg agaggttgat ggggttgtgg 22380 ttgcgggtgt tgatctctgt gggagtgctg agaaccttta cgtcaagtca aggcgtttca 22440 aggtgtctac atctgacacc ccgagagcca gctttgatgc agctgctgat ggttactttg 22500 ttggagaggg ctgtggtgca tttgtgctca agcgtgagac aagctgcacc aaggatgaca 22560 gaatctatgc ttgcatggat gccatagttc ctggaaatgt tccatctgca tgtttgagag 22620 aggcattgga tcaagccaga gtcaagcctg gagacatcga gatgttggag ctttctgctg 22680 attcagcgag gcatttgaag gacccgtctg ttctcccaaa ggaactcact gctgaggaag 22740 agattggcgg acttcagacg attcttcgtg acgatgacaa gctcccaagg aatgttgcaa 22800 ctggaagtgt caaggccact gttggtgaca ctggttatgc ttctggtgct gccagtttga 22860 ttaaggctgc gctttgcatc tacaaccgtt acctgcccag caatggagat gactgggatg 22920 aacctgcacc tgaagcaccc tgggacagca cactctttgc gtgccagacc tcaagagctt 22980 ggttgaagaa ccctggtgag agacgttatg cggctgtttc cggagtcagt gaaaccagaa 23040 gttgctacag tgtgctgctt tctgaagcag agggacacta cgagagagag aacagaatca 23100 gtttggatga ggaagcaccc aagctcattg tgttgagagc agactcccat gaagagattc 23160 ttggaagatt ggacaagata agggaaaggt tcttgcagcc cacgggtgct gcacccagag 23220 agtctgaatt gaaggcgcaa gcccgtagga tctttcttga actccttggt gagacccttg 23280 cccaagatgc agcttcatct ggaagtcaga agccccttgc tctcagcttg gtctccactc 23340 catccaagct ccagagagag gtggaacttg cggcaaaagg cattccgaga tgtctcaaga 23400 tgagacgtga ttggagttcc cctgctggaa gcagatatgc gcctgagcct ttggccagtg 23460 acagagttgc cttcatgtat ggagaaggaa ggagccctta ctatggcatc acacaagaca 23520 ttcataggat ttggcctgaa cttcatgagg tcatcaatga gaaaacaaac cgtctctggg 23580 ctgaaggtga cagatgggtc atgccaaggg ccagcttcaa gagtgagctt gagtcacaac 23640 agcaagagtt tgacagaaac atgattgaaa tgttccgtct tggaatcctc acctcaattg 23700 ccttcacaaa cctggcgaga gatgttctca acatcacgcc caaagcagcc tttggactca 23760 gtttgggaga gatttccatg atctttgctt tctccaagaa aaacggtttg ataagcgatc 23820 agttgacaaa ggatttgaga gagtcagatg tctggaacaa agctctggct gttgagttca 23880 atgcgctcag agaagcctgg ggcattccac agtctgtccc aaaagatgag ttctggcaag 23940 gttacatagt gaggggaacg aaacaagaca tagaagcagc catagcacca gactcaaagt 24000 atgtgaggtt gaccatcatc aatgatgcca acactgctct catcagtgga aagcctgatg 24060 catgcaaagc tgcgattgct cgtcttggtg gcaacatccc tgcgcttcca gtcactcaag 24120 gcatgtgtgg gcattgccca gaggtgggac cttacaccaa ggacatcgca aagatccacg 24180 ccaaccttga gttccctgtg gttgatggtc ttgatctctg gactaccata aaccagaaga 24240 ggcttgtgcc aagagccacg ggtgccaaag atgaatgggc tccttcatcc tttggggagt 24300 atgctggaca gctctatgag aaacaagcca acttccctca gatagttgaa accatctaca 24360 agcagaacta tgatgtgttt gttgaagttg ggccaaacaa tcacagaagc acagcagtga 24420 gaaccacgct tggaccacag agaaaccacc ttgctggtgc catagacaag cagaacgagg 24480 atgcttggac gacaatagtc aagctcgtgg cttcattgaa ggctcacctt gttcctggag 24540 tgaccatcag tccgctctac cactccaagc tcgttgcaga ggctgaggct tgttatgctg 24600 cgctctgtaa aggtgagaaa cccaagaaaa acaagtttgt gagaaagatt cagttgaatg 24660 gcagattcaa cagcaaggca gaccccatct cctcagcaga ccttgcatcc ttcccacctg 24720 cggaccctgc gattgaagct gccatctctt caaggatcat gaagcctgtg gctccgaaat 24780 tctatgcgcg tctcaacatt gatgagcaag atgagaccag agatccgatc ttgaacaagg 24840 acaatgcgcc ttccagctcc agctcttcca gcagttcctc ttccagcagt tcatctccgt 24900 ctcctgctcc ttcagctcca gttcagaaga aagctgcccc agctgcggag acaaaagctg 24960 ttgccagtgc tgatgcactt cgtagtgccc tgcttgatct tgactccatg cttgcgctca 25020 gctctgcaag tgcttctggc aaccttgttg agactgcgcc atctgatgcc agtgtcattg 25080 tccctccgtg caacattgct gatcttggca gcagagcttt catgaaaact tatggtgttt 25140 ctgcgcctct gtacaccggt gcaatggcca agggaattgc ctctgcggac ttggtgatcg 25200 cagctggaag gcaaggaatc cttgcgtcat ttggtgctgg aggccttcca atgcaagttg 25260 tgcgtgagtc cattgaaaag attcaagctg ccctgcccaa tggaccatat gctgtcaacc 25320 tcatccattc tccttttgac agcaaccttg agaagggaaa tgttgatctc ttcttggaaa 25380 agggtgtcac ttttgttgag gcttcagcct tcatgacgtt gacaccgcaa gttgtcagat 25440 acagagcggc tgggttgacg aggaatgcag atgggtcagt caacataagg aacagaatca 25500 ttgggaaagt ctccagaact gagctggctg aaatgttcat gcgtcctgca cctgagcatc 25560 ttttgcagaa gctcattgct tctggggaga tcaatcaaga acaagctgag ttggccagac 25620 gtgttcctgt ggctgatgac attgcggttg aagctgattc tggtggacac actgacaacc 25680 gtccaatcca tgtcattctg cctctgatta tcaacctgag ggataggctt cacagagagt 25740 gtggataccc agcaaacctg agggttcgtg ttggtgctgg gggtggcatt gggtgtcccc 25800 aagctgcgct ggcaactttc aacatgggtg cctctttcat tgtcactgga acagtgaacc 25860 aagttgccaa acaatctggg acgtgtgaca atgtgaggaa acagcttgcg aaagcaactt 25920 actcagatgt ttgcatggca ccggcagctg acatgtttga ggaaggtgtg aaacttcaag 25980 ttttgaagaa aggaacaatg ttcccttcaa gagccaacaa actctatgag ttgttctgca 26040 agtatgattc ttttgagtcc atgcctccag cagagcttgc gagggttgag aaaagaatct 26100 tcagtcgtgc attggaagag gtctgggatg agaccaagaa cttctacatc aaccgtctcc 26160 acaacccaga gaagatacag agggctgaga gggaccccaa actgaagatg tcactgtgtt 26220 tcagatggta tctgtccctg gcttcaaggt gggccaacac tggagcttct gacagagtca 26280 tggactacca agtttggtgt ggtcctgcca ttggttcatt caatgatttc atcaaaggaa 26340 cttaccttga tccggcagtg gcaaatgagt acccgtgtgt tgtgcagatc aacaaacaga 26400 ttcttagagg agcgtgcttc ttgaggcgtc ttgaaatact gagaaatgca aggctttcag 26460 atggtgcagc tgcacttgtg gcgagcattg atgacacata tgtccctgct gagaagctgt 26520 gagtagttag cttaatcact taggtcacct cgagtatcaa aatctattta gaaatacaca 26580 atattttgtt gcaggcttgc tggagaatcg atctgctatc ataaaaatta caaaaaaatt 26640 ttatttgcct caattatttt aggattggta ttaaggacgc ttaaattatt tgtcgggtca 26700 ctacgcatca ttgtgattga gaagatcagc gatacgaaat attcgtagta ctatcgataa 26760 tttatttgaa aattcataag aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga 26820 cagataaagc cacgcacatt taggatattg gccgagatta ctgaatattg agtaagatca 26880 cggaatttct gacaggagca tgtcttcaat tcagcccaaa tggcagttga aatactcaaa 26940 ccgccccata tgcaggagcg gatcattcat tgtttgtttg gttgcctttg ccaacatggg 27000 agtccaaggt tggcgcgccg aattcgcggc cgcttaatta atctgacctg accactagac 27060 ctgagtgagt acagatgtgc tactagtgct agcctcgagg tcgacaccca gctttcttgt 27120 acaaagtggt tgcggccgct taattaaatt taaattcaat taatgcaatc ttgattttca 27180 acaacgaagg taatggcgta aaagaaaaaa tgtatgttat tgtattgatc tttcatgatg 27240 ttgaagcgtg ccataatatg atgatgtata attaaaatat taactgtcgc attttattga 27300 aatggcactg ttatttcaac catatctttg attctgttac atgacacgac tgcaagaagt 27360 aaataataga cgccgttgtt aaagaattgc tatcatatgt gcctaactag agggaatttg 27420 agcgtcagac ctaatcaaat attacaaaat atctcactct gtcgccagca atggtgtaat 27480 cagcgcagac aaatggcgta aagatcgcgg aaaaacctcc ccgagtggca tgatagctgc 27540 ctctgtattg ctgatttagt cagccttatt tgacttaagg gtgccctcgt tagtgacaaa 27600 ttgctttcaa ggagacagcc atgccccaca ctttgttgaa aaacaaattg cctttgggga 27660 gacggtaaag ccagttgctc ttcaataagg aatgtcgagg aggcaatgta accgcctctg 27720 gtagtacact tctctaatcc aaaaatcaat ttgtattcaa gataccgcaa aaaacttatg 27780 gtttaaaccc tgcaggacta gtccagaagg taattatcca agatgtagca tcaagaatcc 27840 aatgtttacg ggaaaaacta tggaagtatt atgtaagctc agcaagaagc agatcaatat 27900 gcggcacata tgcaacctat gttcaaaaat gaagaatgta cagatacaag atcctatact 27960 gccagaatac gaagaagaat acgtagaaat tgaaaaagaa gaaccaggcg aagaaaagaa 28020 tcttgaagac gtaagcactg acgacaacaa tgaaaagaag aagataaggt cggtgattgt 28080 gaaagagaca tagaggacac atgtaaggtg gaaaatgtaa gggcggaaag taaccttatc 28140 acaaaggaat cttatccccc actacttatc cttttatatt tttccgtgtc atttttgccc 28200 ttgagttttc ctatataagg aaccaagttc ggcatttgtg aaaacaagaa aaaatttggt 28260 gtaagctatt ttctttgaag tactgaggat acaacttcag agaaatttgt aagtttgtag 28320 gtaccagatc tggatcccaa accatgtctc cggagaggag accagttgag attaggccag 28380 ctacagcagc tgatatggcc gcggtttgtg atatcgttaa ccattacatt gagacgtcta 28440 cagtgaactt taggacagag ccacaaacac cacaagagtg gattgatgat ctagagaggt 28500 tgcaagatag atacccttgg ttggttgctg aggttgaggg tgttgtggct ggtattgctt 28560 acgctgggcc ctggaaggct aggaacgctt acgattggac agttgagagt actgtttacg 28620 tgtcacatag gcatcaaagg ttgggcctag gatctacatt gtacacacat ttgcttaagt 28680 ctatggaggc gcaaggtttt aagtctgtgg ttgctgttat aggccttcca aacgatccat 28740 ctgttaggtt gcatgaggct ttgggataca cagcccgggg tacattgcgc gcagctggat 28800 acaagcatgg tggatggcat gatgttggtt tttggcaaag ggattttgag ttgccagctc 28860 ctccaaggcc agttaggcca gttacccaaa tctgagtagt tagcttaatc acctagagct 28920 cgatcggcgg caatagcttc ttagcgccat cccgggttga tcctatctgt gttgaaatag 28980 ttgcggtggg caaggctctc tttcagaaag acaggcggcc aaaggaaccc aaggtgaggt 29040 gggctatggc tctcagttcc ttgtggaagc gcttggtcta aggtgcagag gtgttagcgg 29100 gatgaagcaa aagtgtccga ttgtaacaag atatgttgat cctacgtaag gatattaaag 29160 tatgtattca tcactaatat aatcagtgta ttccaatatg tactacgatt tccaatgtct 29220 ttattgtcgc cgtatgtaat cggcgtcaca aaataatccc cggtgacttt cttttaatcc 29280 aggatgaaat aatatgttat tataattttt gcgatttggt ccgttatagg aattgaagtg 29340 tgcttgaggt cggtcgccac cactcccatt tcataatttt acatgtattt gaaaaataaa 29400 aatttatggt attcaattta aacacgtata cttgtaaaga atgatatctt gaaagaaata 29460 tagtttaaat atttattgat aaaataacaa gtcaggtatt atagtccaag caaaaacata 29520 aatttattga tgcaagttta aattcagaaa tatttcaata actgattata tcagctggta 29580 cattgccgta gatgaaagac tgagtgcgat attatggtgt aatacatagg aattcgttta 29640 aacgatctgc gtctaatttt cggtccaact tgcacaggaa agacgtcgac cgcggtagct 29700 cttgcccagc agactgggct tccagtcctt tcgctcgatc gggtccaatg ttgtcctcag 29760 ctgtgaaccg gaagcggacg accaacagtg gaagaactga aaggaacgag ccgtctatac 29820 cttgatgatc ggcctctggt gaagggtatc atcgcagcca agcaagctca tgaaaggctg 29880 atgggggagg tgtataatta tgaggcccac ggcgggctta ttctttaggg aggatctatc 29940 tcgttgctca agtgcatggc gcaaagcagt tattggagtg cggattttcg ttggcatatt 30000 attcgccacg agttagcaga cgaagagacc ttcatgaacg tggccaaggc cagagttaag 30060 cagatgttac gccctgctgc aggcctttct attatccaat agttggttga tctttggaaa 30120 gagcctcggc tgaggcccat actgaaagag atcgatggat atcgatatgc catgttgttt 30180 gctagccaga accagatcac atccgatatg ctattgcagc ttgacgcaga tatggaggat 30240 aagttgattc atgggatcgc tcaggagtag ctcatccatg cacgccgaca agaacagaaa 30300 ttccgtcgag ttaacgcagc cgcttacgac ggattcgaag gtcatccatt cggaatgtat 30360 tagtttgcac cagctccgcg tcacacctgt cttcatttga ataagatgtt agcaattgtt 30420 tttagctttg tcttgttgtg gcagggcggc aagtgcttca gacatcattc tgttttcaaa 30480 ttttatgctg gagaacagct tcttaattcc tttggaaata atagactgcg tcttaaaatt 30540 cagatgtctg gatatagata tgattgtaaa ataacctatt taagtgtcat ttagaacata 30600 agttttatga atgttcttcc attttcgtca tcgaacgaat aagagtaaat acaccttttt 30660 taacattaca aataagttct tatacgttgt ttatacaccg ggaatcattt ccattatttt 30720 cgcgcaaaag tcacggatat tcgtgaaagc gacataaact gcgaaatttg cggggagtgt 30780 cttgagtttg cctcgaggct agcgcatgca catagacaca cacatcatct cattgatgct 30840 tggtaataat tgtcattaga ttgtttttat gcatagatgc actcgaaatc agccaatttt 30900 agacaagtat caaacggatg tgacttcagt acattaaaaa cgtccgcaat gtgttattaa 30960 gttgtctaag cgtcaatttg atttacaatt gaatatatcc tgccccagcc agccaacagc 31020 tcgatttaca attgaatata tcctgccggc cggcccacgc gtgtcgagga attctgatct 31080 ggcccccatt tggacgtgaa tgtagacacg tcgaaataaa gatttccgaa ttagaataat 31140 ttgtttattg ctttcgccta taaatacgac ggatcgtaat ttgtcgtttt atcaaaatgt 31200 actttcattt tataataacg ctgcggacat ctacattttt gaattgaaaa aaaattggta 31260 attactcttt ctttttctcc atattgacca tcatactcat tgctgatcca tgtagatttc 31320 ccggacatga agccatttac aattgaatat atcctgccgc cgctgccgct ttgcacccgg 31380 tggagcttgc atgttggttt ctacgcagaa ctgagccggt taggcagata atttccattg 31440 agaactgagc catgtgcacc ttccccccaa cacggtgagc gacggggcaa cggagtgatc 31500 cacatgggac ttttaaacat catccgtcgg atggcgttgc gagagaagca gtcgatccgt 31560 gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg caaagtattt gaacgcaggt 31620 acaatcgagc cgacgttcac gcggaacgac caagcaagct tggctgccat ttttggggtg 31680 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc 31740 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc 31800 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg 31860 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga 31920 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt 31980 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc 32040 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc 32100 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc 32160 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc 32220 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca 32280 cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc 32340 gagggcaacc agcccggtga gcgtcggaaa gggtcgacgg atcttttccg ctgcataacc 32400 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac 32460 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg 32520 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc 32580 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt 32640 aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc 32700 tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc 32760 cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt 32820 cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg 32880 cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac 32940 gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat 33000 gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg 33060 tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc 33120 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct 33180 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt 33240 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt 33300 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt 33360 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct 33420 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc 33480 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg 33540 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga 33600 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg 33660 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata 33720 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt 33780 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg 33840 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac 33900 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct 33960 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga 34020 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg 34080 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga 34140 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc 34200 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt 34260 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca 34320 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg 34380 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt 34440 gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc agcgctttac 34500 tggcatttca ggaacaagcg ggcactgctc gacgcacttg cttcgctcag tatcgctcgg 34560 gacgcacggc gcgctctacg aactgccgat aaacagagga ttaaaattga caattgtgat 34620 taaggctcag attcgacggc ttggagcggc cgacgtgcag gatttccgcg agatccgatt 34680 gtcggccctg aagaaagctc cagagatgtt cgggtccgtt tacgagcacg aggagaaaaa 34740 gcccatggag gcgttcgctg aacggttgcg agatgccgtg gcattcggcg cctacatcga 34800 cggcgagatc attgggctgt cggtcttcaa acaggaggac ggccccaagg acgctcacaa 34860 ggcgcatctg tccggcgttt tcgtggagcc cgaacagcga ggccgagggg tcgccggtat 34920 gctgctgcgg gcgttgccgg cgggtttatt gctcgtgatg atcgtccgac agattccaac 34980 gggaatctgg tggatgcgca tcttcatcct cggcgcactt aatatttcgc tattctggag 35040 cttgttgttt atttcggtct accgcctgcc gggcggggtc gcggcgacgg taggcgctgt 35100 gcagccgctg atggtcgtgt tcatctctgc cgctctgcta ggtagcccga tacgattgat 35160 ggcggtcctg ggggctattt gcggaactgc gggcgtggcg ctgttggtgt tgacaccaaa 35220 cgcagcgcta gatcctgtcg gcgtcgcagc gggcctggcg ggggcggttt ccatggcgtt 35280 cggaaccgtg ctgacccgca agtggcaacc tcccgtgcct ctgctcacct ttaccgcctg 35340 gcaactggcg gccggaggac ttctgctcgt tccagtagct ttagtgtttg atccgccaat 35400 cccgatgcct acaggaacca atgttctcgg cctggcgtgg ctcggcctga tcggagcggg 35460 tttaacctac ttcctttggt tccgggggat ctcgcgactc gaacctacag ttgtttcctt 35520 actgggcttt ctcagccccc gagcgcttag tgggaatttg taccccttat cgaaccggga 35580 gcacaggatg acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt 35640 caggagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag 35700 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc 35760 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc 35820 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct 35880 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac 35940 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat 36000 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg 36060 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat 36120 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg 36180 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac 36240 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc 36300 ctgccggccc agtatcagcc cgtcatactt gaagctaggc aggcttatct tggacaagaa 36360 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg ttcactacgt gaaaggcgag 36420 atcaccaagg tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg 36480 gcgcggctta actcaagcgt tagagagctg gggaagacta tgcgcgatct gttgaaggtg 36540 gttctaagcc tcgtcttgcg atggcatttc gatccattcc cattccgcgc tcaagatggc 36600 ttcccctcgg cagttcatca gggctaaatc aatctagccg acttgtccgg tgaaatgggc 36660 tgcactccaa cagaaacaat caaacaaaca tacacagcga cttattcaca cgagctca 36718 <210> 66 <211> 36517 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB108209 <400> 66 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcatc agtaagtgca tataaagaaa gtaataagta 300 gatgagtttg gtaattacat gagatcgaca gaggaattac gacgttcttg ccatcaaaaa 360 gagattccaa atttgtaaag agccatatgt ttgttgggca attgagattg agtcttgcca 420 atccttcttt ggaagttgac aatttaaaaa ttcgaagcca gatttactcc taattaatta 480 ttgagcttga agtcttaatt accgattaca tctctgagca gtagtagtcg atgatctttc 540 ccggtcacta attttactca agcgaatcga tttttacttt agtttagcta accgtacgcc 600 attacagttt agagagcagc aagggtaaga acattattaa aacataaagt aaacctctca 660 tttattacgg tgtagtgcta attacattag cctcaaacat cctaagctta cagttttata 720 aaacacacac gatgactgtg tatagtatta acatacatca atagcaacac tcatgatcat 780 cgtgctaggc cgggcttgga ctgggtgacc taagtgatta agctaactac tcagaacgca 840 agtgagtctg tcgcaatgac agaggcttga acatcaatga agagatcacc ttgttcgttg 900 tggaactgaa gtgcatgttt gtgctggcta tgtccaccac tctgattgga tctgagggtg 960 atgtagaagg gtttgtcttg tgggaccggt ctgtgttgga ctatcctctg aatggagttc 1020 gggagtgcag cttgtcccag cgttctcctc acccagacca acattgcttg aaaggcaaga 1080 tcattgacaa agggatcatg tgcatcagtg tcagttgcaa actctcctct ggctgcatct 1140 gatccaggaa cagcagaaca cttggcaaca agctggctct tggtgcagct gagaacgtca 1200 tcaatgcctc tgaacgcagg accatgaaag agagtcttgc catcatagac tgacccttgc 1260 aaagcaggat ctgcatcaag gctcggaggc tgcatggttg cattggcagg tggagcacct 1320 tggttggaca acactatgac agctctgtag gctggaacaa gttttccact tgagaacgtc 1380 ttgagcgtcg cttggacatt caccctccca ctcggagcag tggacggtgt gagggtcact 1440 tcacagttga cgtctccatc aacagtgaca cctttgaaga gctgtgcgtc atctatggcc 1500 cagagagagt agccagggaa gagtccaagg caggtctcag caaggctccc aatagcaagg 1560 gtcattggca acacccttct gccttgtatg acatgatcct ccaagaaggg attggacttg 1620 gcactgatct ttctatgcag ggtgatcgtg tcacttccaa ctttcttgga tggtgtcctc 1680 cagtttccaa caaggatctc agcaggactt gatcccaaca caatcctcgc aactgtatca 1740 gcacctcctt cacgaggaat gatctgaact cccatttctt gaaactgttt cttgagttga 1800 ggtgtcacca tcccaccatc ccagggacca aagcagatgg acttgacact gacatccttc 1860 gcaagttcca aacccatctt gttgagagct tcgtttgcca tcgcatagtc agactgacca 1920 acattgccgt ggaaaccagc aagggagctg aagagaacca tgtgtttgag gttggcacga 1980 tcaactgcag ccagaagatt ctccaaccca gtgactttgg tcccaaagac agcatcaaac 2040 tcgtcaggga gtttcttctc tatgagacga tcacggagca caccagaagc gtgaacgatt 2100 ccactaactc tggcaccaag ctgggactct gcatccctaa ctgctttggc aacatctgca 2160 gcagagttca catcacaaga gctgtagatc gctttgccac ccagagcttc aatggcagca 2220 atggagcttc tcacttcacg agcaccaaga acagatccca caagtttcgt gacagccctt 2280 ggagtgggtt tgggtccttc accagcactg aaggccctct tcaactcttg ggtggcagct 2340 ttctgcacag ccttctcatc agtgatgcca gcacaccatg ctggttcaga cgcagaaact 2400 ttgctacgac ccaacagaat gtacttgcct ccagcaatct gacgcgtgat ctcacggatg 2460 caaagaggtg tgatcccacg agcacctcca gaaaccagaa gcacatcgtc tttcgctatc 2520 tgtctctgag ggttcccagt ctccaacttg gcagctctga ttgtgcatct ttgctggttt 2580 gctccaatcc caacctctct gattctgatg tcagcacacg ccatctccct aactatggca 2640 actgcagcat cttctgggtg catcccttga gcaatgtcca caccacggct gaacacatca 2700 gattctgacc actccaaccc tattgtcttg cagagtccaa agatcgctcc acgctgtgct 2760 ctcttgagtg cgtcagaagt gccttgagag gtgaatccaa gtctgccgtc aagcctagcc 2820 acaccgatga aggcaggacg acctccagcc acagcagtgc agagagaggc tttggcaaac 2880 ttcgcacaca tgagtgtgaa acccagtatc tcagctggtt caaacctttc tgcttgctgg 2940 cttatgaagc ctcccaacgc tccaaatctt tgctcagcct ctttgatggc cttctcagct 3000 gattctgctg aaaggtcctt agccagaaca tgacgaatgg cagctgcacc agctctctga 3060 gccaactgca atccctcaaa ggtcaagaca actgcacagg ctcccaacac cctcacaaga 3120 gcaagtgtca actctgagcc atcgtcaacg acaagaactg gacggttctc cggtctttcc 3180 agaatcagtt catctggcct tctgatgtca accacctttg cgtgcatcag tgacaaatcg 3240 tcacagtcag caccatgaac tgaatcaatc ttcacagctg gtgcagcacc ggcacttgca 3300 ggagcggctg cagccggagc aggggctgat ccaccagcta tctcagcctt catggcatcg 3360 accacttctc caaccgttct ggtccttgac aaagcatcga catctttggc ctcaacgttc 3420 agcatggctt gaacctcaga aaggatttcc acacgcttga tgctatcaat cccaagctct 3480 gtttccaact ccatgtctga ctctatcatg tcagtctcgt atccagtctt tgcagccaaa 3540 acctccatca caacagtttc agctttttca aggagttcgt tgctgacagc tggtgccgcg 3600 gcagcgggag caggagcggc tgcagctggc gcaggagcag aacccccagc tatctcagcc 3660 ttcatggcat caaccacctc tcccaccgtc cttgttctgg aaagagcatc aacatctttg 3720 gcctccacat tcaacatcgc ttgaacctca gagaggattt caacacgttt gatggagtca 3780 attccaagct cggtctccag ttccatgtca gattcaatca tgtcggtctc atagccagtt 3840 ttcgcagcaa ggacttccat cacaactgtc tcagcctttt ccaggagttc tgaggagacg 3900 gccggtgcgg ctgcagctgg agccggtgcc gcggctgcgg gtgcaggagc ggctgcagct 3960 ggcgcaggcg cggaacttcc agcgatctcc gccttcatcg catcaacgac ctcaccaact 4020 gttctggtcc ttgagagggc atccacatcc ttagcctcca cattgagcat tgcttggact 4080 tcactgagga tctcaacacg cttgatgctg tcaatgccca attccgtctc aagttccatg 4140 tcagactcaa tcatgtcagt ctcatagccg gtttttgcag ccagcacttc catgacaact 4200 gtttcagcct tctcaagcaa ttcgttgctg acagctggtg ccgcggctgc gggtgcaggt 4260 gcggcagctg ccggcgcagg agcagagcct ccagcaatct cagccttcat cgcatcgaca 4320 acttccccca cagttcttgt cctactgagt gcatcaacat ctttagcctc aacgttgagc 4380 atggcttgca cctcagagag gatctccact ctcttgatgg aatctattcc aagttcggtc 4440 tccaattcca tgtctgactc aatcatgtcc gtttcgtaac cagtttttgc agcaaggact 4500 tccatcacaa ctgtttcggc tttctccagc aactcattgc tgacggcagg agcggctgca 4560 gctggagcag gtgccgcggc agcaggtgca ggtgcggctg cagctggcgc actagcagag 4620 cttccggcaa tttcagcctt catggcgtcc acgacctcac cgactgttct ggttcttgaa 4680 agcgcatcga catcttttgc ctccacgttc aacatagctt ggacctcaga caagatttca 4740 acacgtttga tgctatctat tcccaactcg gtttcaagct ccatgtcact ttcaatcatg 4800 tcggtctcgt aaccagtctt agctgcgagc acctccatca caaccgtctc ggccttttcc 4860 aggagttcgg atgaaacagc tggcgcgggc gcggccggtg ctggtgcagc tggagctgga 4920 gctgcggctg ccggtgcagg tgccgcggca gctggcgcag gcgcgctccc tccagcaatc 4980 tccgccttca tggcatccac aacctcaccc actgtccttg ttcttgaaag tgcatcaaca 5040 tccttagctt caacattgag catggcttgg acttcactca ggatctccac tcttttgatg 5100 gaatcgatcc caagctctgt ctccaattcc atatcagatt caatcatgtc ggtctcatag 5160 ccagtctttg cagccaaaac ctccatcaca accgtctcag ccttctccaa gagttctgaa 5220 gaaacggcag gtgcaggtgc agctggagca ggtgccgcgg ctgcgggtgc aggagcggct 5280 gcagctggcg cgggcgcaga ccctccagca atttcagcct tcatggcatc cacaacctca 5340 ccaacggtcc tcgttctgga cagagcatca acatccttcg cttccacgtt cagcatagct 5400 tggacctcac tgaggatttc aacacgtttg atgctgtcta tcccaagttc tgtctccaat 5460 tccatgtcag actcaatcat gtcagtctca tagccggttt tagctgccaa gacctccatc 5520 acgacggtct cagctttttc aaggagttcg tttgagacgg ccggagcggc tgcagctgga 5580 gctggagccg cggcagcggg accaggagcg gcagcggccg gcgcaggagc ggagcctcca 5640 gctatctcag ccttcatggc attgacaacc tcaccgacag ttctggtcct tgagagagca 5700 tccacatcct tagcttccac attgagcatg gcttggactt cactgaggat ttccacacgc 5760 ttgatggaat caattccaag ctccgtctca agctccatgt ctgattctat catgtcagtt 5820 tcgtagccag ttttggcagc aaggacttcc atcacaacag tttctgcctt ttcgagcagc 5880 tcattagaaa cagctggtgc agctgcagct ggagccgctt tagccggtgc aggtgcggca 5940 gctgccggcg ccggagcgga agaaccagcg atttctgcct tcatagcgtt gacaacttca 6000 cccactgttc tcgtcctaga gagagcatca acatctttcg cctcaacgtt aagcatagct 6060 tggacttcag aaagaatttc aaccctcttg atgctgtcaa ttcccaactc agtctcaagc 6120 tccatgtccg cctcaatcat gtctgtttcg tatccagtct tggcagcgag aacctccatc 6180 acaactgtct cagctttctc aagcaactcg ttggacacgg cgggagcagg agcacttgca 6240 acgggagcgg caggggcagc ggctttaaca ggcgccggag cagtctgttg ctgttgctgt 6300 tgctgaaggg aagaagcatc aacacttcca taaccatcca gctcagccag catggacttg 6360 aggatggcac gatgtttctc cacaacagca gtgtcaactg caggcttggc tgaagctgca 6420 gcagtcttgg cttcctcctt ggcagcagca agtttggagt tggcttctgc agctgcacgt 6480 ttggcgtcgt caagctgcct ctgagcgtct tgaagctcct tctgaagcct ttcagcctcc 6540 ctcttggcag cttcgtcaac cacaggttca ggagccttga tgagtggtgc agcacccttg 6600 aggtatgtga cacatcttcc gtcgttcatg gcagcatccc tcactttctt ggtcttgtca 6660 ctgacatagg tggcagctga aagtctgagt gtggttctct ttttcttgat ggcttgcatc 6720 ctcgtggcat caggagcatc ccacttgtca aacccttgaa gattgacacc agccacaacc 6780 agctgaactg cagcatccct caactgtatg tcagagtccg tgccagaagc tgggttcaca 6840 gacactgtga caacagacgg gtcatctttg agggtttcag aaacaagttt gctgagaact 6900 tgtttcggtc caaactcaac aaagattctc gcaccagctt ggtgcatgtt cctcacttgg 6960 gtcaggaact tcacagatga ggtcatgtgt tgagtgagca tctctctggc atccgtcgga 7020 taggtctctc cagaaacatt gctgaagagc tttgtttctg ctttgggtgt acggaaggaa 7080 accttggata tgacatcttt gaaagctgag ctggcatttt ccatctgtgg agaatggaag 7140 gcagactcac aagcaagagg cacaactctg aacccttcct tctgcaatct ggcactttca 7200 gcttgaatcc cttcaacaga cccagtaatg acagtctgag acggagagtt tgagtttcca 7260 agccagacgt tggcagcttg aaccttgatg ttctctgcgt tgggtcctat gacagctgcc 7320 atgcaacctt tgggtgtggc aggtgcatct ttgcctccca tgatccttgc ccttctgcaa 7380 acaagttcaa acaactcatc tctgtcaaca caaccagctg catagagcgc agcaaactca 7440 cccaagctgt gtccagctgc aaagtctgga gtgaatccag cttccttgaa aatctcaaag 7500 gcaccaagag cacatgccag ggttgacggc tggctgtatg ctgtgagtga gattttcttg 7560 tgatcttgtt ctggctcacg ttcgtacggt ttccttgggt acaggacttg agagactctt 7620 tcaaagtctt tgtcacttcc agcaaccttg gactgtgcag cgtccattgc agcaatggac 7680 tgtctgaact gaggccagtt catcgcaact tcactgaaca tgtgtgtgta ctgagcacct 7740 tgtccagaga agagtgcagc aacagcacca ttggtggcaa tgcctttggc acggaaagaa 7800 actccttctc tcggaagcct ccacgcttct ttggtcacat ctttggcaaa ctgagcacat 7860 atagcacgaa gcgtggagca cgcatcctcc gcatctttga ccaagaatcc cagtctcgca 7920 ttggtcgcag gtatgctccc agggaacttg aactgttctc caaacttgac acacttgatg 7980 taggcagtat tcttgactgt ctcattctcc ttgatcgcag cttcaaactc tttcagctga 8040 gcttcacaaa ggctctgcaa cgcagcagga gttgcagcca tcatcaacac cggttgtgga 8100 cgtttgttga gtctgtacgc tgtcgtgtgc tctggttcag cctcttccaa cactgcatgg 8160 tagttggcac ctccaaagcc aaagctggat attccagcac gtctcggaac accaggtgga 8220 gggaaccagg gtctgttcat cgtgttgatg tagagggatg actcattgat gggagtgttg 8280 tcatagagat tgggtggatt gtccacattg atcgttccag gcaaagtctt gtgtttgaga 8340 gccatgatga ccttgatcat tccagcaaga ccagcaacag ccttcaaatg accaatactg 8400 gacttgatac ttccaactgc aactttctct gtgttccctt caccatacgc tttgtcaaac 8460 aagttcctca acgcagtcaa ctcaatccta tctccaactg gagtgccagt cccatgaccc 8520 tcaaccaaag tgacagtggc agggtcaacg caggcacgat tgtatgcacg tctcaaggct 8580 tcctcttgac cactgattgt gggagtgtag atgccagctg ctttgccatc acttgagctt 8640 gcacaaccac gaatcacagc atgaatctca tcaccatccc tcactgcatc tgcataacgt 8700 ttgagcacca acatggctga tccctcacca atgagcatcc ctttcgtttt ctcatcatac 8760 gctctcacac ttgggtccgt gctgaacaca ggtgtctttg agaaagccat gtacatccca 8820 atggagttgt ctgtgcaggt tgcaccagtg accatcatgt cgcagtcacc atagagcagt 8880 tcatcaatcg caaccttgac tgctatcaag gagcttgcac atgcagcatc cacaacgcag 8940 ttcatgccat ccaagttgaa tgtgttggtg catctgccag cagtgacatt cccaaggaat 9000 ccagggaaag agtcaagtct ccattctggg aagttcgctt tgtacttctc cacagccact 9060 ttgacatctt cctctggcat ccccatcttt ctcaagactt tctccacaac cacatagttg 9120 agtctgctgt agaactcatg tgaactcttc tgtccacctc caatgccaag cacgcatcct 9180 atgtttttct tctcctttcc aagtgcatca atcccagcat cttgaagtgc ttctttgacc 9240 ttgagaagtg agatggtctg attcgcatca gaatcttcca tctggaacat gttgagacca 9300 aactcacgcg catcaaagtc atactcagga atgaaacctc ctctcttgca gtagattttg 9360 tcctttgtgg tcttgactgg gtcaaagtac gcagtcacat caacacgatc ctcaggaaga 9420 tcagacaggc aatcaatgcc agcccttatg gtctcccatg actctctcac cgtggttcca 9480 caaggaagta tggcgctcat gcctatgatc gcaatcctcg tgtccatctc acctcctttc 9540 tgctcttgca gcctcgctgc catttttggt tgtggatccg ttttgctatt tgtgtatgtt 9600 ttcttgtttt ggtttgagtt taaccatcat tctcttctct ctatttatag agagaagagg 9660 ttggcacgca gcactgcgac atgagagaaa gacacgggtg aagaggcggg agctgaggtg 9720 gcagcatgca tgcatgcatg gccagtcgtg tcatagtgag aagaggagaa agctgaggtg 9780 gcaacgtgta tggccagtgg tgtcatagtg tgaaagctga cgtggcagca tgcatggtga 9840 gtgtgaaaaa ggcgccacag tgcagatgtt actgaacaaa agcggtgtgc catgcaactt 9900 ttgcccatcg tcgtctttct catccaagtt cctctttata atatctttta ccaaatcata 9960 ttttaatata aattcatttt atttttaaat aaagtaatat tatatccata aatattaact 10020 ctcatttaac acattttcaa atttaacact tctgtaaata aatttattaa caaaaacttg 10080 taaatatgtt caaataaatc cataaatatt aactttcatt ttattaacaa aaaaatcttt 10140 actataagct gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtgctagcct cgaggtcgac acaactttgt atacaaaagt tgtctaggat 10260 ctgcttttac atgcgagaca catcttctaa agtaatttta ataatagtta ctatattcaa 10320 gatttcatat atcaaatact caatattact tctaaaaaat taattagata taattaaaat 10380 attacttttt taattttaag tttaattgtt gaatttgtga ctattgattt attattctac 10440 tatgtttaaa ttgttttata gatagtttaa agtaaatata agtaatgtag tagagtgtta 10500 gagtgttacc ctaaaccata aactataaga tttatggtgg actaattttc atatatttct 10560 tattgctttt accttttctt ggtatgtaag tccgtaactg gaattactgt gggttgccat 10620 gacactctgt ggtcttttgg ttcatgcatg gatcttgcgc aagaaaaaga caaagaacaa 10680 agaaaaaaga caaaacagag agacaaaacg caatcacaca accaactcaa attagtcact 10740 ggctgatcaa gatcgccgcg tccatgtatg tctaaatgcc atgcaaagca acacgtgctt 10800 aacatgcact ttaaatggct cacccatctc aacccacaca caaacacatt gcctttttct 10860 tcatcatcac cacaaccacc tgtatatatt cattctcttc cgccacctca atttcttcac 10920 ttcaacacac gtcaacctgc atatgcgtgt catcccatgc ccaaatctcc atgcatgttc 10980 caaccacctt ctctcttata taatacctat aaatacctct aatatcactc acttctttca 11040 tcatccatcc atccagagta ctactactct actactataa taccccaacc caactcatat 11100 tcaatactac tctaggatcc acaaccaaaa atgcttcagc acacttggct tccgaagcct 11160 cccaatctga ccctcttgtc agatgaggtt catctctgga ggattcctct tgaccagcct 11220 gagtcacaac ttcaagacct tgctgccacc ctgagcagtg atgaattggc gagggcaaac 11280 agattctact ttccagaaca cagaaggcgt ttcactgctg ggagaggcat cctcagatcc 11340 atcttgggtg gatacttggg agtggaaccg ggtcaagtca agtttgatta tgagtcccgt 11400 gggaaaccga tccttggtga cagatttgct gagagtggac tcctgttcaa cttgtctcac 11460 agccagaacc ttgccttgtg tgctgtcaac tacacgcgtc aaataggcat tgatcttgaa 11520 tatctgcgtc caacatctga cttggagtct cttgcaaaga ggttctttct cccaagagaa 11580 tatgaactct tgaggtcact ccctgatgag cagaaacaga agattttctt tcgttactgg 11640 acttgcaaag aggcttatct caaagcaacg ggagatggaa tagccaaact tgaagagatc 11700 gagatagcac tcaccccaac agaacctgcc aagctccaaa cagctcctgc gtggtctctg 11760 ttggagcttg tgccagatga caattgtgtt gcagctgtgg ctgttgcggg ttttggttgg 11820 cagcccaagt tctggcatta ctgagtagtt agcttaatca cttaggtcac ctcgagtatc 11880 aaaatctatt tagaaataca caatattttg ttgcaggctt gctggagaat cgatctgcta 11940 tcataaaaat tacaaaaaaa ttttatttgc ctcaattatt ttaggattgg tattaaggac 12000 gcttaaatta tttgtcgggt cactacgcat cattgtgatt gagaagatca gcgatacgaa 12060 atattcgtag tactatcgat aatttatttg aaaattcata agaaaagcaa acgttacatg 12120 aattgatgaa acaatacaaa gacagataaa gccacgcaca tttaggatat tggccgagat 12180 tactgaatat tgagtaagat cacggaattt ctgacaggag catgtcttca attcagccca 12240 aatggcagtt gaaatactca aaccgcccca tatgcaggag cggatcattc attgtttgtt 12300 tggttgcctt tgccaacatg ggagtccaag gttggcgcgc cgaattcgcg gccgcttaat 12360 taactactgt cactgaggcc gtagacgagt acggactgat ctaactagaa ccggtgacta 12420 ctctacagcc ttggattagg cacggcatcg cgatctcttg ggccgttgac ttttaacttg 12480 cgatgacttt tctaatttac gagtcaataa ccattctccc tcagatattg acttgttcgt 12540 agataagtgt cactttggaa gctagcatta cgagtacacg ggtcggcaac ttgcaaatcg 12600 tcgaaaattg ctatgcaagt tgatctctga tttggtagta cactcagctt tgtgatcagc 12660 caatactcat tacgtggttt attagcaaaa tgaatcgaca tgagccacag cgagtgacac 12720 cagttacgat tccctcacaa cctccgtctc gtgaaacgta atagatcagc gttgtatgat 12780 gtaagatatt gtagttttgg atgtcaacgt agggtcgtga agaggtttat taactcacat 12840 gttggaaccc ttagtgagtt ttcactttac aaatcacgcg tgccactacg tattacttca 12900 tcgtagatag ggacaacaca gaaacgaaag tgcgaaacct ttacatcacc aggtcccagg 12960 cgagattccc aggggtgact aaaagtacgc atttgaaaac tatgggggct attgaatcta 13020 aacatcagat agtcaccatg atcaatatcc ccgttacgct tgcttcgtaa tctgggggag 13080 tagccgcttc ccgcgcttac gagcgtgctc aagggccata caaactgacc ccagtgagcc 13140 gttcagagtg cgtgccattg agaattggcg tacttctgtt gaaaaaagtg cgataacgct 13200 cttcagggtg gtcgataacc caagcgtaga atcacacttt gcagaggata tgaggttctt 13260 aactaggata cttggggcga tcatcaagta aggatgaagt cgcgtacgta ctttctacat 13320 gctcagctaa agtgcaagcg ggggacatga cgagatgatt ccaggaatcg gggacattgg 13380 cttagggctt atagatgtct tgttcgataa caccggtgga tccccgggta ccgagctcga 13440 attcctcgac caacttttct atacaaagtt gtctagacct aggactaggc actgagtgac 13500 tcactgacct gactgagtga ctggcggccg catcagtaag tgcatataaa gaaagtaata 13560 agtagatgag tttggtaatt acatgagatc gacagaggaa ttacgacgtt cttgccatca 13620 aaaagagatt ccaaatttgt aaagagccat atgtttgttg ggcaattgag attgagtctt 13680 gccaatcctt ctttggaagt tgacaattta aaaattcgaa gccagattta ctcctaatta 13740 attattgagc ttgaagtctt aattaccgat tacatctctg agcagtagta gtcgatgatc 13800 tttcccggtc actaatttta ctcaagcgaa tcgattttta ctttagttta gctaaccgta 13860 cgccattaca gtttagagag cagcaagggt aagaacatta ttaaaacata aagtaaacct 13920 ctcatttatt acggtgtagt gctaattaca ttagcctcaa acatcctaag cttacagttt 13980 tataaaacac acacgatgac tgtgtatagt attaacatac atcaatagca acactcatga 14040 tcatcgtgct aggccgggct tggactgggt gacctaagtg attaagctaa ctactcaaag 14100 cgcattcgtt ggctcataga caaaggcagc atcttcagtc tcaagatcaa tcctcggatc 14160 atttctcaga gcattaagac gcctcaggta acatgcacca cgcaaaatct gaaggttgat 14220 ctggacaaca catgggtact cattagagac agcaggatca agatatgttc ctttgatgaa 14280 gtcattgaag gctccaatgg ctggaccaca ccagacttga tagtccatga cacgatcagg 14340 agcacccatg ttggcccaac gtgatgcaag accaagatac catctgaaac acaaagacat 14400 cttgagtttg gggtcatgtt ccgctctctg tatcttctct ggattcttca aaccattgat 14460 gtaaaagtcc ttggtctctt cccagacctc ttgaagtgcc ctcttgaaga tcctcttctc 14520 gatcctttca agttcagcag gaggcattga atcgaaagag tcgtacttgc aaaagagttc 14580 gtacaatttg ttagctcttg atgggaacat tgttcccttc ttgagaactt ggagtttcac 14640 accttcctca aacatatcag ctgctggagc catgcaaatg tcactgtagg tggcttgact 14700 gagttgtttt ctcacgttgt cacatgtccc ggactgttta gcaacttgat tgacagtccc 14760 agtcactatg aaggcagctc ccattgtgag ggcagccgca gcagcttgtg ggcagccaac 14820 accgcctcca gctccaactc tgactctaag gtgcgcagga taaccacact ctctgtgcag 14880 tctgttccta aggttgatga tcaaaggcaa gatgacgtgg atgggacggt tgtcagtgtg 14940 accaccagaa tcagcttcaa cagcgatgtc gtctgcgaca ggaacacgac gggcaagttc 15000 ggcttgttct tgtgttatct caccagaagc tatgagtttc tcaaggaggt gttccggagc 15060 cggtctgatg aacatttcgg cgagttctgt tctggacact tttccaatga tacggtttct 15120 gatgttgaca ctcccatcag cgttccttga gagtccggct gcacggtaac gaacaacttg 15180 gggagtgagg gtcatgaatg cagaagcctc caccacagta acgccctttt cgaggaagag 15240 atcaacattc cccttttcca agttgctgtc aaaaggtgaa tgaatgaggt tcacagcata 15300 tggtccttga ggcagagccg cttgtatctt ctccaaagct gccctcacat ggtgcattgg 15360 aagcccaccc gctccaaaag aacccaatat cttcctcttc ccagcagcaa tgacaaggtc 15420 cgcagacgca attcccttgg ccattgctcc agtgtacaga ggtgctgaca cattgtacgt 15480 ttccatgaag gacttgtcac caagatcact tatggagcat ggtttgatac tgacaatcct 15540 tgaagaagtt gcaggtttgg attcaaactg caccaaattg gagttttcaa caccaagata 15600 caatggattg tccacttcaa gcaggacatc tctcaactga gtcggactga gtgcaactcc 15660 agttttccca gggtccgctt ttgcgctggg tggctgttgt tgaacatccg gaatggcttg 15720 aggttttgca ctgacaacac gaactctgag ttctttggct tcatacacac ggagaccatc 15780 aacccaaagt gctccatctg ccactatgtc aacgtagcct ccattttcag gagaggctgt 15840 gattgaaact atgtgcacct cacagtccat cgcacggttc tttggtgtca attgaccacg 15900 gtacttccac gccgtttttc caggggcatg ggcaaaggtc ggattgctca cattgtattc 15960 tcccggtatg ttctggtcca ccgcaaatgc ctcaatgagc tggaacattg actcaattcc 16020 caaagatcca ggcatcacag gatcaaacca aaagtgacaa gagaagaacc agtcttgtgg 16080 gttaacatct ttgtgtccat gcgcatatcc tttcccatgc agcccagaat caggagcaat 16140 gttgaggtaa tccaaatact cacactgttc tgaccttctg agaacttgag cattagttgc 16200 atctggaagt ttctcagtca gctgttcttt gcctttggct gatgcgattt caactcttct 16260 cacactcgtg ttgttagttt tgcaccaagg ctccgtcttt ttcccattgt caagtccagc 16320 ttgctgagca aacacctctg gagtgaacca tccaaaggaa gtgcttcctt tgtagaaaac 16380 cacgccatca acagacaact caaacgtgaa tctgtgaatc cccatctttc ccaacattgc 16440 atatccagtg cacttggtca cattccttat ggttttcccc ctcacatcca catctggcct 16500 caccatttca gcacttgcat caaggttcct aaagagaatg tcatctttgt ccatcgtcag 16560 tggtgctttc aacacactgg tgagaacacc ggatgtctga agtccgattt ccatgatgat 16620 ggaataaggc atgtgcccat cattgcaaga accatcaaag aaccacgcat cttgaggaca 16680 gtcaaactcc cccaccattg tgcctttgct gggattgcaa tcaacattca ggaacttgcc 16740 atgttccatg tttgtgactt caacaactct agtcacaaga gccaaatcaa aagcagggct 16800 tctgctcgta tttgacttat caaatcttgc gaactctggt cccaagcagt tgctaacttt 16860 tccacacatg aactctgaca tgttgtacca gttcagaggc atttctccag ctttgcaatt 16920 gttgtcaagt gggttgccag ggaaagggat gaagcaaatt gccctcggag ggtaagaact 16980 aggggagaag aacggcgtcg ggttgccagg aagttttgac atgggatgcc aggacatttc 17040 tttcggagcg agagcgtcac ccttgaggat atgttcagga agtgcaactg gctttggcgc 17100 aagaacatct gctgagaaag caggcttcgc aggggcagca gaagcactag cactggcacc 17160 agagctggcc ttggacgctt ccggtcccac agtggctgca ccgttagcaa aaacgggctg 17220 aactttggag gggttcttgt tcgtggatct cttctgcatc ttgagagcaa tgcccttgaa 17280 gtccacgacg atctttttgt taagatcccc ctttccgtag tcgctgattc tatcaaggct 17340 gaaatcttga cccttttcga aatcaacgtc aatgatgttg acgtcagcaa tggcgtacgg 17400 atcgttatcc tcgtcgaatc ccatctcttt gatctccatg acgtagacca actttccctt 17460 atgaggagat atctgacccc tacacctaac tttgttcggg tgtccgttga cgggtctgaa 17520 gtcaaatggt ccggtggtaa ggtgcaaacc gagccagatc atgtacatct tgagcatttg 17580 tgaacaaccg tcgctgacaa gagatccagc catgacttga tccttaacaa aatggcatgg 17640 gaagtaccaa tggtccctct ccaagatttt ctcaccaacc aactgaccca aaccgtacac 17700 acctccctta tggtcaatgc tggtgactct atcaatcatg agcatcttac gtgcgcagag 17760 tttgtagttg atctccggca tgccattctt tgagccaaaa acggaagccc aatctttgtc 17820 aaccaaggtc tgcatctcct tttcgttgag ttttgttttg tgaaggcagg gagcgacagc 17880 atagggagaa acatcttgct ttggaatctt agctctggca gccaaatccc ctcttgtgaa 17940 caccacaccc tttcccgcat caagctcctc atttgtgaag aagccagcac agccatctct 18000 catctcaatg agaagcctcc cattgacata gcagtcatac tcaaagaaga acatggagat 18060 gccaccatcc aaacgtttgg caaaaccggt cactctgatg tcatactcaa gggtctctcc 18120 ctcatgggca accccataga atgtcaacgt ggtgttgagc agacggtaaa ctctgtcacc 18180 ttggttctgg aaatcaattc ccatgtatga tatcagcatc aaatcgcact gtccactctc 18240 aaccagaact gcccagggac aatcacctcc ctcagacaat tcgccattca ctggaagatc 18300 atactcagtg accattctgg caccaactct gtagttgttg acctcggcat ccatgagtgt 18360 gacccttgtc acaagcaggt attcacgagc aggcagcctc acccttctgg ggtacttgtc 18420 tatgacagca aactccggac caaaaacctt agctatgtca ccctctgcaa actccaggag 18480 ttcctcatag ttgaacacct cagttttgcc agaggtcagt tcctccttgg tcatctccca 18540 acatggcttc ttgttggtct tgacacgaag cgccattttt ggttgtggat ccgttttgct 18600 atttgtgtat gttttcttgt tttggtttga gtttaaccat cattctcttc tctctattta 18660 tagagagaag aggttggcac gcagcactgc gacatgagag aaagacacgg gtgaagaggc 18720 gggagctgag gtggcagcat gcatgcatgc atggccagtc gtgtcatagt gagaagagga 18780 gaaagctgag gtggcaacgt gtatggccag tggtgtcata gtgtgaaagc tgacgtggca 18840 gcatgcatgg tgagtgtgaa aaaggcgcca cagtgcagat gttactgaac aaaagcggtg 18900 tgccatgcaa cttttgccca tcgtcgtctt tctcatccaa gttcctcttt ataatatctt 18960 ttaccaaatc atattttaat ataaattcat tttattttta aataaagtaa tattatatcc 19020 ataaatatta actctcattt aacacatttt caaatttaac acttctgtaa ataaatttat 19080 taacaaaaac ttgtaaatat gttcaaataa atccataaat attaactttc attttattaa 19140 caaaaaaatc tttactataa gctgcggccg cttaattaat cacgagtgga tgactgtcac 19200 gactgactca tgactgacta ctagtgctag cctcgaggtc gacacaactt tgtataataa 19260 agttgtctag tctgctttta catgcgagac acatcttcta aagtaatttt aataatagtt 19320 actatattca agatttcata tatcaaatac tcaatattac ttctaaaaaa ttaattagat 19380 ataattaaaa tattactttt ttaattttaa gtttaattgt tgaatttgtg actattgatt 19440 tattattcta ctatgtttaa attgttttat agatagttta aagtaaatat aagtaatgta 19500 gtagagtgtt agagtgttac cctaaaccat aaactataag atttatggtg gactaatttt 19560 catatatttc ttattgcttt taccttttct tggtatgtaa gtccgtaact ggaattactg 19620 tgggttgcca tgacactctg tggtcttttg gttcatgcat ggatcttgcg caagaaaaag 19680 acaaagaaca aagaaaaaag acaaaacaga gagacaaaac gcaatcacac aaccaactca 19740 aattagtcac tggctgatca agatcgccgc gtccatgtat gtctaaatgc catgcaaagc 19800 aacacgtgct taacatgcac tttaaatggc tcacccatct caacccacac acaaacacat 19860 tgcctttttc ttcatcatca ccacaaccac ctgtatatat tcattctctt ccgccacctc 19920 aatttcttca cttcaacaca cgtcaacctg catatgcgtg tcatcccatg cccaaatctc 19980 catgcatgtt ccaaccacct tctctcttat ataataccta taaatacctc taatatcact 20040 cacttctttc atcatccatc catccagagt actactactc tactactata ataccccaac 20100 ccaactcata ttcaatacta ctctaggatc cacaaccaaa aatggctgca aggaacgtga 20160 gtgcggctca tgagatgcat gacgagaaga ggatagctgt tgtgggcatg gctgttcagt 20220 atgctggatg caaaacgaaa gacgagttct gggaagttct catgaatgga aaagttgaga 20280 gcaaagtgat cagcgacaaa cgtcttggtt caaattaccg tgctgaacac tacaaagctg 20340 aaaggagcaa gtatgctgac accttctgca atgagacata tggaactctt gatgagaacg 20400 aaatagacaa tgagcatgaa ctccttttga accttgccaa acaagccttg gctgaaacat 20460 ctgtcaaaga ttcaacaaga tgtggcatag tttctggatg cctcagcttc ccgatggaca 20520 acctccaagg tgaactcttg aatgtgtacc agaaccatgt tgagaagaaa cttggtgcca 20580 gagttttcaa agatgcctct cactggtcag aaagagaaca gtccaacaaa ccagaggctg 20640 gtgacagaag aatcttcatg gaccctgctt cctttgttgc tgaggaactc aatcttggcg 20700 ctctccacta ctctgttgat gctgcctgtg ccactgcgct ctatgtgctc agactggcgc 20760 aagatcactt ggtctctggt gcagctgatg tgatgctttg tggtgcaaca tgcctgccgg 20820 agccgttttt catactctct gggttctcaa ccttccaagc catgcctgtt ggaacgggtc 20880 agaatgtgtc catgccgctg cacaaagatt ctcaaggatt gacccctggt gaaggaggtt 20940 ccatcatggt cctcaaacgt ttggatgacg caatcagaga tggagatcac atctatggaa 21000 cacttttggg agccaatgtc agcaacagtg gcactggtct gccacttaaa ccccttttgc 21060 cgtcagagaa aaagtgcctc atggacacct acacacgtat caatgtgcat ccccacaaga 21120 ttcagtatgt tgaatgccat gcaactggga ctccacaagg tgatcgtgtg gaaattgatg 21180 ctgtgaaggc ttgctttgaa ggcaaagtcc cacgttttgg aaccacaaaa gggaactttg 21240 gacacacatt ggtggcagct gggtttgctg gcatgtgcaa agtgcttctc tccatgaagc 21300 acgggatcat tccacccact ccgggaatag atgacgaaac gaagatggac cctctggttg 21360 tctctggtga ggccattccc tggccagaaa cgaatggaga accgaagcgt gctggtctct 21420 cagcgtttgg atttggtggg accaatgctc atgctgtctt tgaggaacat gacccaagca 21480 atgctgcatg cactggccat gactccattt ctgctctctc tgcacgttgt ggtggtgagt 21540 caaacatgag aatagcaatc actgggatgg atgccacttt tggtgctttg aaaggattgg 21600 atgcttttga aagggccatc tacactggtg ctcatggtgc tataccactc ccagaaaaga 21660 gatggagatt cctggggaaa gacaaggact tccttgatct ttgtggagtc aaagcaacac 21720 cgcatggctg ttacattgaa gatgttgaag ttgatttcca gaggctgagg actccaatga 21780 ctcctgaaga tatgctcttg cctcaacagc ttctggctgt caccacaatt gatagggcca 21840 tattggactc tggcatgaag aaaggtggaa atgtggctgt ctttgtgggg ttggggaccg 21900 atcttgaact ctaccgtcac cgtgcaaggg ttgctctcaa ggagagagtg aggcctgaag 21960 ccagcaagaa actcaatgac atgatgcagt acatcaatga ttgtgggaca agcacttctt 22020 acaccagcta cataggcaac ttggttgcaa caagggtcag ttcacaatgg ggcttcacgg 22080 gaccttcctt cacaatcaca gaaggcaaca attctgtgta cagatgtgct gaacttggca 22140 aatacctctt ggaaacggga gaggttgatg gggttgtggt tgcgggtgtt gatctctgtg 22200 ggagtgctga gaacctttac gtcaagtcaa ggcgtttcaa ggtgtctaca tctgacaccc 22260 cgagagccag ctttgatgca gctgctgatg gttactttgt tggagagggc tgtggtgcat 22320 ttgtgctcaa gcgtgagaca agctgcacca aggatgacag aatctatgct tgcatggatg 22380 ccatagttcc tggaaatgtt ccatctgcat gtttgagaga ggcattggat caagccagag 22440 tcaagcctgg agacatcgag atgttggagc tttctgctga ttcagcgagg catttgaagg 22500 acccgtctgt tctcccaaag gaactcactg ctgaggaaga gattggcgga cttcagacga 22560 ttcttcgtga cgatgacaag ctcccaagga atgttgcaac tggaagtgtc aaggccactg 22620 ttggtgacac tggttatgct tctggtgctg ccagtttgat taaggctgcg ctttgcatct 22680 acaaccgtta cctgcccagc aatggagatg actgggatga acctgcacct gaagcaccct 22740 gggacagcac actctttgcg tgccagacct caagagcttg gttgaagaac cctggtgaga 22800 gacgttatgc ggctgtttcc ggagtcagtg aaaccagaag ttgctacagt gtgctgcttt 22860 ctgaagcaga gggacactac gagagagaga acagaatcag tttggatgag gaagcaccca 22920 agctcattgt gttgagagca gactcccatg aagagattct tggaagattg gacaagataa 22980 gggaaaggtt cttgcagccc acgggtgctg cacccagaga gtctgaattg aaggcgcaag 23040 cccgtaggat ctttcttgaa ctccttggtg agacccttgc ccaagatgca gcttcatctg 23100 gaagtcagaa gccccttgct ctcagcttgg tctccactcc atccaagctc cagagagagg 23160 tggaacttgc ggcaaaaggc attccgagat gtctcaagat gagacgtgat tggagttccc 23220 ctgctggaag cagatatgcg cctgagcctt tggccagtga cagagttgcc ttcatgtatg 23280 gagaaggaag gagcccttac tatggcatca cacaagacat tcataggatt tggcctgaac 23340 ttcatgaggt catcaatgag aaaacaaacc gtctctgggc tgaaggtgac agatgggtca 23400 tgccaagggc cagcttcaag agtgagcttg agtcacaaca gcaagagttt gacagaaaca 23460 tgattgaaat gttccgtctt ggaatcctca cctcaattgc cttcacaaac ctggcgagag 23520 atgttctcaa catcacgccc aaagcagcct ttggactcag tttgggagag atttccatga 23580 tctttgcttt ctccaagaaa aacggtttga taagcgatca gttgacaaag gatttgagag 23640 agtcagatgt ctggaacaaa gctctggctg ttgagttcaa tgcgctcaga gaagcctggg 23700 gcattccaca gtctgtccca aaagatgagt tctggcaagg ttacatagtg aggggaacga 23760 aacaagacat agaagcagcc atagcaccag actcaaagta tgtgaggttg accatcatca 23820 atgatgccaa cactgctctc atcagtggaa agcctgatgc atgcaaagct gcgattgctc 23880 gtcttggtgg caacatccct gcgcttccag tcactcaagg catgtgtggg cattgcccag 23940 aggtgggacc ttacaccaag gacatcgcaa agatccacgc caaccttgag ttccctgtgg 24000 ttgatggtct tgatctctgg actaccataa accagaagag gcttgtgcca agagccacgg 24060 gtgccaaaga tgaatgggct ccttcatcct ttggggagta tgctggacag ctctatgaga 24120 aacaagccaa cttccctcag atagttgaaa ccatctacaa gcagaactat gatgtgtttg 24180 ttgaagttgg gccaaacaat cacagaagca cagcagtgag aaccacgctt ggaccacaga 24240 gaaaccacct tgctggtgcc atagacaagc agaacgagga tgcttggacg acaatagtca 24300 agctcgtggc ttcattgaag gctcaccttg ttcctggagt gaccatcagt ccgctctacc 24360 actccaagct cgttgcagag gctgaggctt gttatgctgc gctctgtaaa ggtgagaaac 24420 ccaagaaaaa caagtttgtg agaaagattc agttgaatgg cagattcaac agcaaggcag 24480 accccatctc ctcagcagac cttgcatcct tcccacctgc ggaccctgcg attgaagctg 24540 ccatctcttc aaggatcatg aagcctgtgg ctccgaaatt ctatgcgcgt ctcaacattg 24600 atgagcaaga tgagaccaga gatccgatct tgaacaagga caatgcgcct tccagctcca 24660 gctcttccag cagttcctct tccagcagtt catctccgtc tcctgctcct tcagctccag 24720 ttcagaagaa agctgcccca gctgcggaga caaaagctgt tgccagtgct gatgcacttc 24780 gtagtgccct gcttgatctt gactccatgc ttgcgctcag ctctgcaagt gcttctggca 24840 accttgttga gactgcgcca tctgatgcca gtgtcattgt ccctccgtgc aacattgctg 24900 atcttggcag cagagctttc atgaaaactt atggtgtttc tgcgcctctg tacaccggtg 24960 caatggccaa gggaattgcc tctgcggact tggtgatcgc agctggaagg caaggaatcc 25020 ttgcgtcatt tggtgctgga ggccttccaa tgcaagttgt gcgtgagtcc attgaaaaga 25080 ttcaagctgc cctgcccaat ggaccatatg ctgtcaacct catccattct ccttttgaca 25140 gcaaccttga gaagggaaat gttgatctct tcttggaaaa gggtgtcact tttgttgagg 25200 cttcagcctt catgacgttg acaccgcaag ttgtcagata cagagcggct gggttgacga 25260 ggaatgcaga tgggtcagtc aacataagga acagaatcat tgggaaagtc tccagaactg 25320 agctggctga aatgttcatg cgtcctgcac ctgagcatct tttgcagaag ctcattgctt 25380 ctggggagat caatcaagaa caagctgagt tggccagacg tgttcctgtg gctgatgaca 25440 ttgcggttga agctgattct ggtggacaca ctgacaaccg tccaatccat gtcattctgc 25500 ctctgattat caacctgagg gataggcttc acagagagtg tggataccca gcaaacctga 25560 gggttcgtgt tggtgctggg ggtggcattg ggtgtcccca agctgcgctg gcaactttca 25620 acatgggtgc ctctttcatt gtcactggaa cagtgaacca agttgccaaa caatctggga 25680 cgtgtgacaa tgtgaggaaa cagcttgcga aagcaactta ctcagatgtt tgcatggcac 25740 cggcagctga catgtttgag gaaggtgtga aacttcaagt tttgaagaaa ggaacaatgt 25800 tcccttcaag agccaacaaa ctctatgagt tgttctgcaa gtatgattct tttgagtcca 25860 tgcctccagc agagcttgcg agggttgaga aaagaatctt cagtcgtgca ttggaagagg 25920 tctgggatga gaccaagaac ttctacatca accgtctcca caacccagag aagatacaga 25980 gggctgagag ggaccccaaa ctgaagatgt cactgtgttt cagatggtat ctgtccctgg 26040 cttcaaggtg ggccaacact ggagcttctg acagagtcat ggactaccaa gtttggtgtg 26100 gtcctgccat tggttcattc aatgatttca tcaaaggaac ttaccttgat ccggcagtgg 26160 caaatgagta cccgtgtgtt gtgcagatca acaaacagat tcttagagga gcgtgcttct 26220 tgaggcgtct tgaaatactg agaaatgcaa ggctttcaga tggtgcagct gcacttgtgg 26280 cgagcattga tgacacatat gtccctgctg agaagctgtg agtagttagc ttaatcactt 26340 aggtcacctc gagtatcaaa atctatttag aaatacacaa tattttgttg caggcttgct 26400 ggagaatcga tctgctatca taaaaattac aaaaaaattt tatttgcctc aattatttta 26460 ggattggtat taaggacgct taaattattt gtcgggtcac tacgcatcat tgtgattgag 26520 aagatcagcg atacgaaata ttcgtagtac tatcgataat ttatttgaaa attcataaga 26580 aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt 26640 aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat 26700 gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg 26760 atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaaggtt ggcgcgccga 26820 attcgcggcc gcttaattaa tctgacctga ccactagacc tgagtgagta cagatgtgct 26880 actagtgcta gcctcgaggt cgacacccag ctttcttgta caaagtggtt gcggccgctt 26940 aattaaattt aaattcaatt aatgcaatct tgattttcaa caacgaaggt aatggcgtaa 27000 aagaaaaaat gtatgttatt gtattgatct ttcatgatgt tgaagcgtgc cataatatga 27060 tgatgtataa ttaaaatatt aactgtcgca ttttattgaa atggcactgt tatttcaacc 27120 atatctttga ttctgttaca tgacacgact gcaagaagta aataatagac gccgttgtta 27180 aagaattgct atcatatgtg cctaactaga gggaatttga gcgtcagacc taatcaaata 27240 ttacaaaata tctcactctg tcgccagcaa tggtgtaatc agcgcagaca aatggcgtaa 27300 agatcgcgga aaaacctccc cgagtggcat gatagctgcc tctgtattgc tgatttagtc 27360 agccttattt gacttaaggg tgccctcgtt agtgacaaat tgctttcaag gagacagcca 27420 tgccccacac tttgttgaaa aacaaattgc ctttggggag acggtaaagc cagttgctct 27480 tcaataagga atgtcgagga ggcaatgtaa ccgcctctgg tagtacactt ctctaatcca 27540 aaaatcaatt tgtattcaag ataccgcaaa aaacttatgg tttaaaccct gcaggactag 27600 tccagaaggt aattatccaa gatgtagcat caagaatcca atgtttacgg gaaaaactat 27660 ggaagtatta tgtaagctca gcaagaagca gatcaatatg cggcacatat gcaacctatg 27720 ttcaaaaatg aagaatgtac agatacaaga tcctatactg ccagaatacg aagaagaata 27780 cgtagaaatt gaaaaagaag aaccaggcga agaaaagaat cttgaagacg taagcactga 27840 cgacaacaat gaaaagaaga agataaggtc ggtgattgtg aaagagacat agaggacaca 27900 tgtaaggtgg aaaatgtaag ggcggaaagt aaccttatca caaaggaatc ttatccccca 27960 ctacttatcc ttttatattt ttccgtgtca tttttgccct tgagttttcc tatataagga 28020 accaagttcg gcatttgtga aaacaagaaa aaatttggtg taagctattt tctttgaagt 28080 actgaggata caacttcaga gaaatttgta agtttgtagg taccagatct ggatcccaaa 28140 ccatgtctcc ggagaggaga ccagttgaga ttaggccagc tacagcagct gatatggccg 28200 cggtttgtga tatcgttaac cattacattg agacgtctac agtgaacttt aggacagagc 28260 cacaaacacc acaagagtgg attgatgatc tagagaggtt gcaagataga tacccttggt 28320 tggttgctga ggttgagggt gttgtggctg gtattgctta cgctgggccc tggaaggcta 28380 ggaacgctta cgattggaca gttgagagta ctgtttacgt gtcacatagg catcaaaggt 28440 tgggcctagg atctacattg tacacacatt tgcttaagtc tatggaggcg caaggtttta 28500 agtctgtggt tgctgttata ggccttccaa acgatccatc tgttaggttg catgaggctt 28560 tgggatacac agcccggggt acattgcgcg cagctggata caagcatggt ggatggcatg 28620 atgttggttt ttggcaaagg gattttgagt tgccagctcc tccaaggcca gttaggccag 28680 ttacccaaat ctgagtagtt agcttaatca cctagagctc gatcggcggc aatagcttct 28740 tagcgccatc ccgggttgat cctatctgtg ttgaaatagt tgcggtgggc aaggctctct 28800 ttcagaaaga caggcggcca aaggaaccca aggtgaggtg ggctatggct ctcagttcct 28860 tgtggaagcg cttggtctaa ggtgcagagg tgttagcggg atgaagcaaa agtgtccgat 28920 tgtaacaaga tatgttgatc ctacgtaagg atattaaagt atgtattcat cactaatata 28980 atcagtgtat tccaatatgt actacgattt ccaatgtctt tattgtcgcc gtatgtaatc 29040 ggcgtcacaa aataatcccc ggtgactttc ttttaatcca ggatgaaata atatgttatt 29100 ataatttttg cgatttggtc cgttatagga attgaagtgt gcttgaggtc ggtcgccacc 29160 actcccattt cataatttta catgtatttg aaaaataaaa atttatggta ttcaatttaa 29220 acacgtatac ttgtaaagaa tgatatcttg aaagaaatat agtttaaata tttattgata 29280 aaataacaag tcaggtatta tagtccaagc aaaaacataa atttattgat gcaagtttaa 29340 attcagaaat atttcaataa ctgattatat cagctggtac attgccgtag atgaaagact 29400 gagtgcgata ttatggtgta atacatagga attcgtttaa acgatctgcg tctaattttc 29460 ggtccaactt gcacaggaaa gacgtcgacc gcggtagctc ttgcccagca gactgggctt 29520 ccagtccttt cgctcgatcg ggtccaatgt tgtcctcagc tgtgaaccgg aagcggacga 29580 ccaacagtgg aagaactgaa aggaacgagc cgtctatacc ttgatgatcg gcctctggtg 29640 aagggtatca tcgcagccaa gcaagctcat gaaaggctga tgggggaggt gtataattat 29700 gaggcccacg gcgggcttat tctttaggga ggatctatct cgttgctcaa gtgcatggcg 29760 caaagcagtt attggagtgc ggattttcgt tggcatatta ttcgccacga gttagcagac 29820 gaagagacct tcatgaacgt ggccaaggcc agagttaagc agatgttacg ccctgctgca 29880 ggcctttcta ttatccaata gttggttgat ctttggaaag agcctcggct gaggcccata 29940 ctgaaagaga tcgatggata tcgatatgcc atgttgtttg ctagccagaa ccagatcaca 30000 tccgatatgc tattgcagct tgacgcagat atggaggata agttgattca tgggatcgct 30060 caggagtagc tcatccatgc acgccgacaa gaacagaaat tccgtcgagt taacgcagcc 30120 gcttacgacg gattcgaagg tcatccattc ggaatgtatt agtttgcacc agctccgcgt 30180 cacacctgtc ttcatttgaa taagatgtta gcaattgttt ttagctttgt cttgttgtgg 30240 cagggcggca agtgcttcag acatcattct gttttcaaat tttatgctgg agaacagctt 30300 cttaattcct ttggaaataa tagactgcgt cttaaaattc agatgtctgg atatagatat 30360 gattgtaaaa taacctattt aagtgtcatt tagaacataa gttttatgaa tgttcttcca 30420 ttttcgtcat cgaacgaata agagtaaata cacctttttt aacattacaa ataagttctt 30480 atacgttgtt tatacaccgg gaatcatttc cattattttc gcgcaaaagt cacggatatt 30540 cgtgaaagcg acataaactg cgaaatttgc ggggagtgtc ttgagtttgc ctcgaggcta 30600 gcgcatgcac atagacacac acatcatctc attgatgctt ggtaataatt gtcattagat 30660 tgtttttatg catagatgca ctcgaaatca gccaatttta gacaagtatc aaacggatgt 30720 gacttcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttga 30780 tttacaattg aatatatcct gccccagcca gccaacagct cgatttacaa ttgaatatat 30840 cctgccggcc ggcccacgcg tgtcgaggaa ttctgatctg gcccccattt ggacgtgaat 30900 gtagacacgt cgaaataaag atttccgaat tagaataatt tgtttattgc tttcgcctat 30960 aaatacgacg gatcgtaatt tgtcgtttta tcaaaatgta ctttcatttt ataataacgc 31020 tgcggacatc tacatttttg aattgaaaaa aaattggtaa ttactctttc tttttctcca 31080 tattgaccat catactcatt gctgatccat gtagatttcc cggacatgaa gccatttaca 31140 attgaatata tcctgccgcc gctgccgctt tgcacccggt ggagcttgca tgttggtttc 31200 tacgcagaac tgagccggtt aggcagataa tttccattga gaactgagcc atgtgcacct 31260 tccccccaac acggtgagcg acggggcaac ggagtgatcc acatgggact tttaaacatc 31320 atccgtcgga tggcgttgcg agagaagcag tcgatccgtg agatcagccg acgcaccggg 31380 caggcgcgca acacgatcgc aaagtatttg aacgcaggta caatcgagcc gacgttcacg 31440 cggaacgacc aagcaagctt ggctgccatt tttggggtga ggccgttcgc ggccgagggg 31500 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg 31560 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag 31620 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg 31680 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag 31740 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct 31800 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca 31860 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca 31920 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga 31980 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc 32040 gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg 32100 cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag 32160 cgtcggaaag ggtcgacgga tcttttccgc tgcataaccc tgcttcgggg tcattatagc 32220 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg 32280 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc 32340 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg 32400 gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat 32460 ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc 32520 agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta 32580 cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg 32640 cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct 32700 caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa 32760 gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc 32820 agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg 32880 tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtattcgtg cagggcaaga 32940 ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg 33000 ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca 33060 ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc 33120 ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca 33180 tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg 33240 tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc 33300 ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg 33360 cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg 33420 gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga 33480 agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga 33540 tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa 33600 tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca 33660 cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg 33720 agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc 33780 aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc 33840 gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt 33900 cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc 33960 tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg 34020 agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc 34080 gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc 34140 gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc 34200 tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg 34260 ggtcagttcc ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg 34320 gcactgctcg acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga 34380 actgccgata aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct 34440 tggagcggcc gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc 34500 agagatgttc gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga 34560 acggttgcga gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc 34620 ggtcttcaaa caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt 34680 cgtggagccc gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc 34740 gggtttattg ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat 34800 cttcatcctc ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta 34860 ccgcctgccg ggcggggtcg cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt 34920 catctctgcc gctctgctag gtagcccgat acgattgatg gcggtcctgg gggctatttg 34980 cggaactgcg ggcgtggcgc tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg 35040 cgtcgcagcg ggcctggcgg gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa 35100 gtggcaacct cccgtgcctc tgctcacctt taccgcctgg caactggcgg ccggaggact 35160 tctgctcgtt ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa 35220 tgttctcggc ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt 35280 ccgggggatc tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagcccccg 35340 agcgcttagt gggaatttgt accccttatc gaaccgggag cacaggatga cgcctaacaa 35400 ttcattcaag ccgacaccgc ttcgcggcgc ggcttaattc aggagttaaa catcatgagg 35460 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc 35520 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg 35580 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg 35640 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt 35700 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca 35760 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc 35820 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc 35880 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta 35940 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat 36000 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc 36060 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc 36120 gtcatacttg aagctaggca ggcttatctt ggacaagaag atcgcttggc ctcgcgcgca 36180 gatcagttgg aagaatttgt tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa 36240 taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt 36300 agagagctgg ggaagactat gcgcgatctg ttgaaggtgg ttctaagcct cgtcttgcga 36360 tggcatttcg atccattccc attccgcgct caagatggct tcccctcggc agttcatcag 36420 ggctaaatca atctagccga cttgtccggt gaaatgggct gcactccaac agaaacaatc 36480 aaacaaacat acacagcgac ttattcacac gagctca 36517 <210> 67 <211> 36590 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9159 <400> 67 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga 900 ggattgcgat cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat 960 gggagaccat aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg 1020 tgactgcgta ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag 1080 gtttcattcc tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg 1140 aagattctga tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg 1200 ctgggattga tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag 1260 gtggacagaa gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag 1320 tcttgagaaa gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag 1380 cgaacttccc agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg 1440 gcagatgcac caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg 1500 caagctcctt gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca 1560 tgatggtcac tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa 1620 agacacctgt gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga 1680 tgctcattgg tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg 1740 atggtgatga gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag 1800 ctggcatcta cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc 1860 gtgcctgcgt tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag 1920 ttggagatag gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag 1980 ggaacacaga gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg 2040 ttgctggtct tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg 2100 gaacgatcaa tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat 2160 ccctctacat caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg 2220 ctggaatatc cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg 2280 aaccagagca cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg 2340 ctgcaactcc tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg 2400 cgatcaagga gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag 2460 aacagttcaa gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca 2520 aagatgcgga ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg 2580 tgaccaaaga agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg 2640 ccaccaatgg tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt 2700 tcagtgaagt tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg 2760 cacagtccaa ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc 2820 caaggaaacc gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat 2880 acagccagcc gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg 2940 gattcactcc agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag 3000 ctggttgtgt tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg 3060 gaggcaaaga tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg 3120 cagagaacat caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc 3180 agactgtcat tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg 3240 aagggttcag agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa 3300 atgccagctc agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag 3360 aaacaaagct cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc 3420 tcactcaaca catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag 3480 ctggtgcgag aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg 3540 aaaccctcaa agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg 3600 actctgacat acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc 3660 aagggtttga caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga 3720 gaaccacact cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg 3780 atgctgccat gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca 3840 aggctcctga acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg 3900 agcttcaaga cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact 3960 ccaaacttgc tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag 4020 ttgacactgc tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg 4080 atggttatgg aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc 4140 cggcgcctgt taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg 4200 tgtccaacga gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga 4260 ctggatacga aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg 4320 acagcatcaa gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga 4380 aagatgttga tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg 4440 cagaaatcgc tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag 4500 cggctccagc tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg 4560 ttgtgatgga agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca 4620 tggagcttga gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag 4680 tccaagccat gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg 4740 tcggtgaggt tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg 4800 ccgctgccgc tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg 4860 tctcaaacga actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa 4920 ccggctatga gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag 4980 acagcatcaa acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga 5040 aggatgttga tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg 5100 ctgaaattgc tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg 5160 cggcacctgc tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg 5220 agacggttgt gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat 5280 ctgatatgga attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga 5340 gtgaagtcca agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa 5400 ggacagtggg tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg 5460 cgccagctgc cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac 5520 cggccgcgcc cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga 5580 tggaggtgct cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc 5640 ttgaaaccga gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag 5700 ctatgttgaa cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg 5760 aggtcgtgga cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag 5820 ccgcacctgc acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca 5880 atgagttgct ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt 5940 acgaaacgga catgattgag tcagacatgg aattggagac cgaacttgga atagattcca 6000 tcaagagagt ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg 6060 ttgatgcact cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga 6120 ttgctggagg ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac 6180 cagctgtcag caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg 6240 caaaaaccgg ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg 6300 gcattgacag catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg 6360 aggctaagga tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga 6420 tgaaggcgga gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg 6480 cagccgcggc accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa 6540 aggctgagac agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga 6600 ttgaatctga catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa 6660 tcctctctga ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca 6720 gaacaaggac ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg 6780 ctcctgcgcc agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg 6840 aactccttga aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg 6900 agactgacat gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca 6960 agcgtgtgga aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg 7020 atgctttgtc aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag 7080 ctggtggatc agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag 7140 ctgtgaagat tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa 7200 aggtggttga catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag 7260 ttcttgtcgt tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct 7320 gtgcagttgt cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca 7380 ttcgtcatgt tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg 7440 ctgagcaaag atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac 7500 cagctgagat actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca 7560 ctgctgtggc tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg 7620 gattcacctc tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg 7680 gactctgcaa gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg 7740 acattgctca agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt 7800 gtgctgacat cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca 7860 gagctgccaa gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc 7920 tggtttctgg aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga 7980 ttgctggagg caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat 8040 ggtgtgctgg catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga 8100 gggccttcag tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg 8160 gatctgttct tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg 8220 gcaaagcgat ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta 8280 gggatgcaga gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc 8340 tccgtgatcg tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca 8400 aagtcactgg gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg 8460 ttctcttcag ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg 8520 caaacgaagc tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca 8580 tctgctttgg tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag 8640 aaatgggagt tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt 8700 tgggatcaag tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg 8760 gaagtgacac gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg 8820 atcatgtcat acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg 8880 agacctgcct tggactcttc cctggctact ctctctgggc catagatgac gcacagctct 8940 tcaaaggtgt cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg 9000 ctccgagtgg gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg 9060 ttccagccta cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa 9120 ccatgcagcc tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca 9180 agactctctt tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga 9240 gccagcttgt tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg 9300 caactgacac tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg 9360 tctgggtgag gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc 9420 aacacagacc ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg 9480 gtggacatag ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca 9540 ttgatgttca agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa 9600 tcacttaggt cacctttaaa cggtcgcagc gtgtgcgtgt ccgtcgtacg ttctggccgg 9660 ccgggccttg ggcgcgcgat cagaagcgtt gcgttggcgt gtgtgtgctt ctggtttgct 9720 ttaattttac caagtttgtt tcaaggtgga tcgcgtggtc aaggcccgtg tgctttaaag 9780 acccaccggc actggcagtg agtgttgctg cttgtgtagg ctttggtacg tatgggcttt 9840 atttgcttct ggatgttgtg tactacttgg gtttgttgaa ttattatgag cagttgcgta 9900 ttgtaattca gctgggctac ctggacattg ttatgtatta ataaatgctt tgctttcttc 9960 taaagatctt taagtgctcg gccggccttg gccacggtgg ccagatccac tagaggcgcg 10020 cctctagtgc ggccgcttaa ttaagatgag tgatactcag gactcaggac tcactctgct 10080 gatcactagt gctagcctcg aggtcgacac aactttgtat acaaaagttg tctagaccta 10140 ggctcagcaa ctcatccagt caagttcgag tcagtgagtt gggcggccgc ctcccagtat 10200 cattatagtg aaagttttgg ctctctcgcc ggtggttttt tacctctatt taaaggggtt 10260 ttccacctaa aaattctggt atcattctca ctttacttgt tactttaatt tctcataatc 10320 tttggttgaa attatcacgc ttccgcacac gatatcccta caaatttatt atttgttaaa 10380 cattttcaaa ccgcataaaa ttttatgaag tcccgtctat ctttaatgta gtctaacatt 10440 ttcatattga aatatataat ttacttaatt ttagcgttgg tagaaagcat aatgatttat 10500 tcttattctt cttcatataa atgtttaata tacaatataa acaaattctt taccttaaga 10560 aggatttccc attttatatt ttaaaaatat atttatcaaa tatttttcaa ccacgtaaat 10620 ctcataataa taagttgttt caaaagtaat aaaatttaac tccataattt ttttattcga 10680 ctgatcttaa agcaacaccc agtgacacaa ctagccattt ttttctttga ataaaaaaat 10740 ccaattatca ttgtattttt tttatacaat gaaaatttca ccaaacaatg atttgtggta 10800 tttctgaagc aagtcatgtt atgcaaaatt ctataattcc catttgacac tacggaagta 10860 actgaagatc tgcttttaca tgcgagacac atcttctaaa gtaattttaa taatagttac 10920 tatattcaag atttcatata tcaaatactc aatattactt ctaaaaaatt aattagatat 10980 aattaaaata ttactttttt aattttaagt ttaattgttg aatttgtgac tattgattta 11040 ttattctact atgtttaaat tgttttatag atagtttaaa gtaaatataa gtaatgtagt 11100 agagtgttag agtgttaccc taaaccataa actataagat ttatggtgga ctaattttca 11160 tatatttctt attgctttta ccttttcttg gtatgtaagt ccgtaactgg aattactgtg 11220 ggttgccatg acactctgtg gtcttttggt tcatgcatgg atcttgcgca agaaaaagac 11280 aaagaacaaa gaaaaaagac aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa 11340 ttagtcactg gctgatcaag atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa 11400 cacgtgctta acatgcactt taaatggctc acccatctca acccacacac aaacacattg 11460 cctttttctt catcatcacc acaaccacct gtatatattc attctcttcc gccacctcaa 11520 tttcttcact tcaacacacg tcaacctgca tatgcgtgtc atcccatgcc caaatctcca 11580 tgcatgttcc aaccaccttc tctcttatat aatacctata aatacctcta atatcactca 11640 cttctttcat catccatcca tccagagtac tactactcta ctactataat accccaaccc 11700 aactcatatt caatactact ctaggatcca caaccaaaaa tggctgcaag gaacgtgagt 11760 gcggctcatg agatgcatga cgagaagagg atagctgttg tgggcatggc tgttcagtat 11820 gctggatgca aaacgaaaga cgagttctgg gaagttctca tgaatggaaa agttgagagc 11880 aaagtgatca gcgacaaacg tcttggttca aattaccgtg ctgaacacta caaagctgaa 11940 aggagcaagt atgctgacac cttctgcaat gagacatatg gaactcttga tgagaacgaa 12000 atagacaatg agcatgaact ccttttgaac cttgccaaac aagccttggc tgaaacatct 12060 gtcaaagatt caacaagatg tggcatagtt tctggatgcc tcagcttccc gatggacaac 12120 ctccaaggtg aactcttgaa tgtgtaccag aaccatgttg agaagaaact tggtgccaga 12180 gttttcaaag atgcctctca ctggtcagaa agagaacagt ccaacaaacc agaggctggt 12240 gacagaagaa tcttcatgga ccctgcttcc tttgttgctg aggaactcaa tcttggcgct 12300 ctccactact ctgttgatgc tgcctgtgcc actgcgctct atgtgctcag actggcgcaa 12360 gatcacttgg tctctggtgc agctgatgtg atgctttgtg gtgcaacatg cctgccggag 12420 ccgtttttca tactctctgg gttctcaacc ttccaagcca tgcctgttgg aacgggtcag 12480 aatgtgtcca tgccgctgca caaagattct caaggattga cccctggtga aggaggttcc 12540 atcatggtcc tcaaacgttt ggatgacgca atcagagatg gagatcacat ctatggaaca 12600 cttttgggag ccaatgtcag caacagtggc actggtctgc cacttaaacc ccttttgccg 12660 tcagagaaaa agtgcctcat ggacacctac acacgtatca atgtgcatcc ccacaagatt 12720 cagtatgttg aatgccatgc aactgggact ccacaaggtg atcgtgtgga aattgatgct 12780 gtgaaggctt gctttgaagg caaagtccca cgttttggaa ccacaaaagg gaactttgga 12840 cacacattgg tggcagctgg gtttgctggc atgtgcaaag tgcttctctc catgaagcac 12900 gggatcattc cacccactcc gggaatagat gacgaaacga agatggaccc tctggttgtc 12960 tctggtgagg ccattccctg gccagaaacg aatggagaac cgaagcgtgc tggtctctca 13020 gcgtttggat ttggtgggac caatgctcat gctgtctttg aggaacatga cccaagcaat 13080 gctgcatgca ctggccatga ctccatttct gctctctctg cacgttgtgg tggtgagtca 13140 aacatgagaa tagcaatcac tgggatggat gccacttttg gtgctttgaa aggattggat 13200 gcttttgaaa gggccatcta cactggtgct catggtgcta taccactccc agaaaagaga 13260 tggagattcc tggggaaaga caaggacttc cttgatcttt gtggagtcaa agcaacaccg 13320 catggctgtt acattgaaga tgttgaagtt gatttccaga ggctgaggac tccaatgact 13380 cctgaagata tgctcttgcc tcaacagctt ctggctgtca ccacaattga tagggccata 13440 ttggactctg gcatgaagaa aggtggaaat gtggctgtct ttgtggggtt ggggaccgat 13500 cttgaactct accgtcaccg tgcaagggtt gctctcaagg agagagtgag gcctgaagcc 13560 agcaagaaac tcaatgacat gatgcagtac atcaatgatt gtgggacaag cacttcttac 13620 accagctaca taggcaactt ggttgcaaca agggtcagtt cacaatgggg cttcacggga 13680 ccttccttca caatcacaga aggcaacaat tctgtgtaca gatgtgctga acttggcaaa 13740 tacctcttgg aaacgggaga ggttgatggg gttgtggttg cgggtgttga tctctgtggg 13800 agtgctgaga acctttacgt caagtcaagg cgtttcaagg tgtctacatc tgacaccccg 13860 agagccagct ttgatgcagc tgctgatggt tactttgttg gagagggctg tggtgcattt 13920 gtgctcaagc gtgagacaag ctgcaccaag gatgacagaa tctatgcttg catggatgcc 13980 atagttcctg gaaatgttcc atctgcatgt ttgagagagg cattggatca agccagagtc 14040 aagcctggag acatcgagat gttggagctt tctgctgatt cagcgaggca tttgaaggac 14100 ccgtctgttc tcccaaagga actcactgct gaggaagaga ttggcggact tcagacgatt 14160 cttcgtgacg atgacaagct cccaaggaat gttgcaactg gaagtgtcaa ggccactgtt 14220 ggtgacactg gttatgcttc tggtgctgcc agtttgatta aggctgcgct ttgcatctac 14280 aaccgttacc tgcccagcaa tggagatgac tgggatgaac ctgcacctga agcaccctgg 14340 gacagcacac tctttgcgtg ccagacctca agagcttggt tgaagaaccc tggtgagaga 14400 cgttatgcgg ctgtttccgg agtcagtgaa accagaagtt gctacagtgt gctgctttct 14460 gaagcagagg gacactacga gagagagaac agaatcagtt tggatgagga agcacccaag 14520 ctcattgtgt tgagagcaga ctcccatgaa gagattcttg gaagattgga caagataagg 14580 gaaaggttct tgcagcccac gggtgctgca cccagagagt ctgaattgaa ggcgcaagcc 14640 cgtaggatct ttcttgaact ccttggtgag acccttgccc aagatgcagc ttcatctgga 14700 agtcagaagc cccttgctct cagcttggtc tccactccat ccaagctcca gagagaggtg 14760 gaacttgcgg caaaaggcat tccgagatgt ctcaagatga gacgtgattg gagttcccct 14820 gctggaagca gatatgcgcc tgagcctttg gccagtgaca gagttgcctt catgtatgga 14880 gaaggaagga gcccttacta tggcatcaca caagacattc ataggatttg gcctgaactt 14940 catgaggtca tcaatgagaa aacaaaccgt ctctgggctg aaggtgacag atgggtcatg 15000 ccaagggcca gcttcaagag tgagcttgag tcacaacagc aagagtttga cagaaacatg 15060 attgaaatgt tccgtcttgg aatcctcacc tcaattgcct tcacaaacct ggcgagagat 15120 gttctcaaca tcacgcccaa agcagccttt ggactcagtt tgggagagat ttccatgatc 15180 tttgctttct ccaagaaaaa cggtttgata agcgatcagt tgacaaagga tttgagagag 15240 tcagatgtct ggaacaaagc tctggctgtt gagttcaatg cgctcagaga agcctggggc 15300 attccacagt ctgtcccaaa agatgagttc tggcaaggtt acatagtgag gggaacgaaa 15360 caagacatag aagcagccat agcaccagac tcaaagtatg tgaggttgac catcatcaat 15420 gatgccaaca ctgctctcat cagtggaaag cctgatgcat gcaaagctgc gattgctcgt 15480 cttggtggca acatccctgc gcttccagtc actcaaggca tgtgtgggca ttgcccagag 15540 gtgggacctt acaccaagga catcgcaaag atccacgcca accttgagtt ccctgtggtt 15600 gatggtcttg atctctggac taccataaac cagaagaggc ttgtgccaag agccacgggt 15660 gccaaagatg aatgggctcc ttcatccttt ggggagtatg ctggacagct ctatgagaaa 15720 caagccaact tccctcagat agttgaaacc atctacaagc agaactatga tgtgtttgtt 15780 gaagttgggc caaacaatca cagaagcaca gcagtgagaa ccacgcttgg accacagaga 15840 aaccaccttg ctggtgccat agacaagcag aacgaggatg cttggacgac aatagtcaag 15900 ctcgtggctt cattgaaggc tcaccttgtt cctggagtga ccatcagtcc gctctaccac 15960 tccaagctcg ttgcagaggc tgaggcttgt tatgctgcgc tctgtaaagg tgagaaaccc 16020 aagaaaaaca agtttgtgag aaagattcag ttgaatggca gattcaacag caaggcagac 16080 cccatctcct cagcagacct tgcatccttc ccacctgcgg accctgcgat tgaagctgcc 16140 atctcttcaa ggatcatgaa gcctgtggct ccgaaattct atgcgcgtct caacattgat 16200 gagcaagatg agaccagaga tccgatcttg aacaaggaca atgcgccttc cagctccagc 16260 tcttccagca gttcctcttc cagcagttca tctccgtctc ctgctccttc agctccagtt 16320 cagaagaaag ctgccccagc tgcggagaca aaagctgttg ccagtgctga tgcacttcgt 16380 agtgccctgc ttgatcttga ctccatgctt gcgctcagct ctgcaagtgc ttctggcaac 16440 cttgttgaga ctgcgccatc tgatgccagt gtcattgtcc ctccgtgcaa cattgctgat 16500 cttggcagca gagctttcat gaaaacttat ggtgtttctg cgcctctgta caccggtgca 16560 atggccaagg gaattgcctc tgcggacttg gtgatcgcag ctggaaggca aggaatcctt 16620 gcgtcatttg gtgctggagg ccttccaatg caagttgtgc gtgagtccat tgaaaagatt 16680 caagctgccc tgcccaatgg accatatgct gtcaacctca tccattctcc ttttgacagc 16740 aaccttgaga agggaaatgt tgatctcttc ttggaaaagg gtgtcacttt tgttgaggct 16800 tcagccttca tgacgttgac accgcaagtt gtcagataca gagcggctgg gttgacgagg 16860 aatgcagatg ggtcagtcaa cataaggaac agaatcattg ggaaagtctc cagaactgag 16920 ctggctgaaa tgttcatgcg tcctgcacct gagcatcttt tgcagaagct cattgcttct 16980 ggggagatca atcaagaaca agctgagttg gccagacgtg ttcctgtggc tgatgacatt 17040 gcggttgaag ctgattctgg tggacacact gacaaccgtc caatccatgt cattctgcct 17100 ctgattatca acctgaggga taggcttcac agagagtgtg gatacccagc aaacctgagg 17160 gttcgtgttg gtgctggggg tggcattggg tgtccccaag ctgcgctggc aactttcaac 17220 atgggtgcct ctttcattgt cactggaaca gtgaaccaag ttgccaaaca atctgggacg 17280 tgtgacaatg tgaggaaaca gcttgcgaaa gcaacttact cagatgtttg catggcaccg 17340 gcagctgaca tgtttgagga aggtgtgaaa cttcaagttt tgaagaaagg aacaatgttc 17400 ccttcaagag ccaacaaact ctatgagttg ttctgcaagt atgattcttt tgagtccatg 17460 cctccagcag agcttgcgag ggttgagaaa agaatcttca gtcgtgcatt ggaagaggtc 17520 tgggatgaga ccaagaactt ctacatcaac cgtctccaca acccagagaa gatacagagg 17580 gctgagaggg accccaaact gaagatgtca ctgtgtttca gatggtatct gtccctggct 17640 tcaaggtggg ccaacactgg agcttctgac agagtcatgg actaccaagt ttggtgtggt 17700 cctgccattg gttcattcaa tgatttcatc aaaggaactt accttgatcc ggcagtggca 17760 aatgagtacc cgtgtgttgt gcagatcaac aaacagattc ttagaggagc gtgcttcttg 17820 aggcgtcttg aaatactgag aaatgcaagg ctttcagatg gtgcagctgc acttgtggcg 17880 agcattgatg acacatatgt ccctgctgag aagctgtgag tagttagctt aatcacttag 17940 gtcaccttta aacggtcgca gcgtgtgcgt gtccgtcgta cgttctggcc ggccgggcct 18000 tgggcgcgcg atcagaagcg ttgcgttggc gtgtgtgtgc ttctggtttg ctttaatttt 18060 accaagtttg tttcaaggtg gatcgcgtgg tcaaggcccg tgtgctttaa agacccaccg 18120 gcactggcag tgagtgttgc tgcttgtgta ggctttggta cgtatgggct ttatttgctt 18180 ctggatgttg tgtactactt gggtttgttg aattattatg agcagttgcg tattgtaatt 18240 cagctgggct acctggacat tgttatgtat taataaatgc tttgctttct tctaaagatc 18300 tttaagtgct cggccggcct tggccacggt ggccagatcc actagaggcg cgcctctagt 18360 gcggccgctt aattaatctg acctgaccac tagacctgag tgagtacaga tgtgctacta 18420 gtgctagcct cgaggtcgac caacttttct atacaaagtt gtctagacct aggactaggc 18480 actgagtgac tcactgacct gactgagtga ctggcggccg cagcttatag taaagatttt 18540 tttgttaata aaatgaaagt taatatttat ggatttattt gaacatattt acaagttttt 18600 gttaataaat ttatttacag aagtgttaaa tttgaaaatg tgttaaatga gagttaatat 18660 ttatggatat aatattactt tatttaaaaa taaaatgaat ttatattaaa atatgatttg 18720 gtaaaagata ttataaagag gaacttggat gagaaagacg acgatgggca aaagttgcat 18780 ggcacaccgc ttttgttcag taacatctgc actgtggcgc ctttttcaca ctcaccatgc 18840 atgctgccac gtcagctttc acactatgac accactggcc atacacgttg ccacctcagc 18900 tttctcctct tctcactatg acacgactgg ccatgcatgc atgcatgctg ccacctcagc 18960 tcccgcctct tcacccgtgt ctttctctca tgtcgcagtg ctgcgtgcca acctcttctc 19020 tctataaata gagagaagag aatgatggtt aaactcaaac caaaacaaga aaacatacac 19080 aaatagcaaa acggatccac aaccaaaaat ggcgcttcgt gtcaagacca acaagaagcc 19140 atgttgggag atgaccaagg aggaactgac ctctggcaaa actgaggtgt tcaactatga 19200 ggaactcctg gagtttgcag agggtgacat agctaaggtt tttggtccgg agtttgctgt 19260 catagacaag taccccagaa gggtgaggct gcctgctcgt gaatacctgc ttgtgacaag 19320 ggtcacactc atggatgccg aggtcaacaa ctacagagtt ggtgccagaa tggtcactga 19380 gtatgatctt ccagtgaatg gcgaattgtc tgagggaggt gattgtccct gggcagttct 19440 ggttgagagt ggacagtgcg atttgatgct gatatcatac atgggaattg atttccagaa 19500 ccaaggtgac agagtttacc gtctgctcaa caccacgttg acattctatg gggttgccca 19560 tgagggagag acccttgagt atgacatcag agtgaccggt tttgccaaac gtttggatgg 19620 tggcatctcc atgttcttct ttgagtatga ctgctatgtc aatgggaggc ttctcattga 19680 gatgagagat ggctgtgctg gcttcttcac aaatgaggag cttgatgcgg gaaagggtgt 19740 ggtgttcaca agaggggatt tggctgccag agctaagatt ccaaagcaag atgtttctcc 19800 ctatgctgtc gctccctgcc ttcacaaaac aaaactcaac gaaaaggaga tgcagacctt 19860 ggttgacaaa gattgggctt ccgtttttgg ctcaaagaat ggcatgccgg agatcaacta 19920 caaactctgc gcacgtaaga tgctcatgat tgatagagtc accagcattg accataaggg 19980 aggtgtgtac ggtttgggtc agttggttgg tgagaaaatc ttggagaggg accattggta 20040 cttcccatgc cattttgtta aggatcaagt catggctgga tctcttgtca gcgacggttg 20100 ttcacaaatg ctcaagatgt acatgatctg gctcggtttg caccttacca ccggaccatt 20160 tgacttcaga cccgtcaacg gacacccgaa caaagttagg tgtaggggtc agatatctcc 20220 tcataaggga aagttggtct acgtcatgga gatcaaagag atgggattcg acgaggataa 20280 cgatccgtac gccattgctg acgtcaacat cattgacgtt gatttcgaaa agggtcaaga 20340 tttcagcctt gatagaatca gcgactacgg aaagggggat cttaacaaaa agatcgtcgt 20400 ggacttcaag ggcattgctc tcaagatgca gaagagatcc acgaacaaga acccctccaa 20460 agttcagccc gtttttgcta acggtgcagc cactgtggga ccggaagcgt ccaaggccag 20520 ctctggtgcc agtgctagtg cttctgctgc ccctgcgaag cctgctttct cagcagatgt 20580 tcttgcgcca aagccagttg cacttcctga acatatcctc aagggtgacg ctctcgctcc 20640 gaaagaaatg tcctggcatc ccatgtcaaa acttcctggc aacccgacgc cgttcttctc 20700 ccctagttct taccctccga gggcaatttg cttcatccct ttccctggca acccacttga 20760 caacaattgc aaagctggag aaatgcctct gaactggtac aacatgtcag agttcatgtg 20820 tggaaaagtt agcaactgct tgggaccaga gttcgcaaga tttgataagt caaatacgag 20880 cagaagccct gcttttgatt tggctcttgt gactagagtt gttgaagtca caaacatgga 20940 acatggcaag ttcctgaatg ttgattgcaa tcccagcaaa ggcacaatgg tgggggagtt 21000 tgactgtcct caagatgcgt ggttctttga tggttcttgc aatgatgggc acatgcctta 21060 ttccatcatc atggaaatcg gacttcagac atccggtgtt ctcaccagtg tgttgaaagc 21120 accactgacg atggacaaag atgacattct ctttaggaac cttgatgcaa gtgctgaaat 21180 ggtgaggcca gatgtggatg tgagggggaa aaccataagg aatgtgacca agtgcactgg 21240 atatgcaatg ttgggaaaga tggggattca cagattcacg tttgagttgt ctgttgatgg 21300 cgtggttttc tacaaaggaa gcacttcctt tggatggttc actccagagg tgtttgctca 21360 gcaagctgga cttgacaatg ggaaaaagac ggagccttgg tgcaaaacta acaacacgag 21420 tgtgagaaga gttgaaatcg catcagccaa aggcaaagaa cagctgactg agaaacttcc 21480 agatgcaact aatgctcaag ttctcagaag gtcagaacag tgtgagtatt tggattacct 21540 caacattgct cctgattctg ggctgcatgg gaaaggatat gcgcatggac acaaagatgt 21600 taacccacaa gactggttct tctcttgtca cttttggttt gatcctgtga tgcctggatc 21660 tttgggaatt gagtcaatgt tccagctcat tgaggcattt gcggtggacc agaacatacc 21720 gggagaatac aatgtgagca atccgacctt tgcccatgcc cctggaaaaa cggcgtggaa 21780 gtaccgtggt caattgacac caaagaaccg tgcgatggac tgtgaggtgc acatagtttc 21840 aatcacagcc tctcctgaaa atggaggcta cgttgacata gtggcagatg gagcactttg 21900 ggttgatggt ctccgtgtgt atgaagccaa agaactcaga gttcgtgttg tcagtgcaaa 21960 acctcaagcc attccggatg ttcaacaaca gccacccagc gcaaaagcgg accctgggaa 22020 aactggagtt gcactcagtc cgactcagtt gagagatgtc ctgcttgaag tggacaatcc 22080 attgtatctt ggtgttgaaa actccaattt ggtgcagttt gaatccaaac ctgcaacttc 22140 ttcaaggatt gtcagtatca aaccatgctc cataagtgat cttggtgaca agtccttcat 22200 ggaaacgtac aatgtgtcag cacctctgta cactggagca atggccaagg gaattgcgtc 22260 tgcggacctt gtcattgctg ctgggaagag gaagatattg ggttcttttg gagcgggtgg 22320 gcttccaatg caccatgtga gggcagcttt ggagaagata caagcggctc tgcctcaagg 22380 accatatgct gtgaacctca ttcattcacc ttttgacagc aacttggaaa aggggaatgt 22440 tgatctcttc ctcgaaaagg gcgttactgt ggtggaggct tctgcattca tgaccctcac 22500 tccccaagtt gttcgttacc gtgcagccgg actctcaagg aacgctgatg ggagtgtcaa 22560 catcagaaac cgtatcattg gaaaagtgtc cagaacagaa ctcgccgaaa tgttcatcag 22620 accggctccg gaacacctcc ttgagaaact catagcttct ggtgagataa cacaagaaca 22680 agccgaactt gcccgtcgtg ttcctgtcgc agacgacatc gctgttgaag ctgattctgg 22740 tggtcacact gacaaccgtc ccatccacgt catcttgcct ttgatcatca accttaggaa 22800 cagactgcac agagagtgtg gttatcctgc gcaccttaga gtcagagttg gagctggagg 22860 cggtgttggc tgcccacaag ctgctgcggc tgccctcaca atgggagctg ccttcatagt 22920 gactgggact gtcaatcaag ttgctaaaca gtccgggaca tgtgacaacg tgagaaaaca 22980 actcagtcaa gccacctaca gtgacatttg catggctcca gcagctgata tgtttgagga 23040 aggtgtgaaa ctccaagttc tcaagaaggg aacaatgttc ccatcaagag ctaacaaatt 23100 gtacgaactc ttttgcaagt acgactcttt cgattcaatg cctcctgctg aacttgaaag 23160 gatcgagaag aggatcttca agagggcact tcaagaggtc tgggaagaga ccaaggactt 23220 ttacatcaat ggtttgaaga atccagagaa gatacagaga gcggaacatg accccaaact 23280 caagatgtct ttgtgtttca gatggtatct tggtcttgca tcacgttggg ccaacatggg 23340 tgctcctgat cgtgtcatgg actatcaagt ctggtgtggt ccagccattg gagccttcaa 23400 tgacttcatc aaaggaacat atcttgatcc tgctgtctct aatgagtacc catgtgttgt 23460 ccagatcaac cttcagattt tgcgtggtgc atgttacctg aggcgtctta atgctctgag 23520 aaatgatccg aggattgatc ttgagactga agatgctgcc tttgtctatg agccaacgaa 23580 tgcgctttga gtagttagct taatcactta ggtcaccttt aaacggtcgc agcgtgtgcg 23640 tgtccgtcgt acgttctggc cggccgggcc ttgggcgcgc gatcagaagc gttgcgttgg 23700 cgtgtgtgtg cttctggttt gctttaattt taccaagttt gtttcaaggt ggatcgcgtg 23760 gtcaaggccc gtgtgcttta aagacccacc ggcactggca gtgagtgttg ctgcttgtgt 23820 aggctttggt acgtatgggc tttatttgct tctggatgtt gtgtactact tgggtttgtt 23880 gaattattat gagcagttgc gtattgtaat tcagctgggc tacctggaca ttgttatgta 23940 ttaataaatg ctttgctttc ttctaaagat ctttaagtgc tcggccggcc ttggccacgg 24000 tggccagatc cactagaggc gcgcctctag tgcggccgct taattaatca cgagtggatg 24060 actgtcacga ctgactcatg actgactact agtgctagcc tcgaggtcga cacaactttg 24120 tataataaag ttgtctagac ctaggagctg tgctgggtaa ctcacatgag tcactggtca 24180 catcagcggc cgcctcccag tatcattata gtgaaagttt tggctctctc gccggtggtt 24240 ttttacctct atttaaaggg gttttccacc taaaaattct ggtatcattc tcactttact 24300 tgttacttta atttctcata atctttggtt gaaattatca cgcttccgca cacgatatcc 24360 ctacaaattt attatttgtt aaacattttc aaaccgcata aaattttatg aagtcccgtc 24420 tatctttaat gtagtctaac attttcatat tgaaatatat aatttactta attttagcgt 24480 tggtagaaag cataatgatt tattcttatt cttcttcata taaatgttta atatacaata 24540 taaacaaatt ctttacctta agaaggattt cccattttat attttaaaaa tatatttatc 24600 aaatattttt caaccacgta aatctcataa taataagttg tttcaaaagt aataaaattt 24660 aactccataa tttttttatt cgactgatct taaagcaaca cccagtgaca caactagcca 24720 tttttttctt tgaataaaaa aatccaatta tcattgtatt ttttttatac aatgaaaatt 24780 tcaccaaaca atgatttgtg gtatttctga agcaagtcat gttatgcaaa attctataat 24840 tcccatttga cactacggaa gtaactgaag atctgctttt acatgcgaga cacatcttct 24900 aaagtaattt taataatagt tactatattc aagatttcat atatcaaata ctcaatatta 24960 cttctaaaaa attaattaga tataattaaa atattacttt tttaatttta agtttaattg 25020 ttgaatttgt gactattgat ttattattct actatgttta aattgtttta tagatagttt 25080 aaagtaaata taagtaatgt agtagagtgt tagagtgtta ccctaaacca taaactataa 25140 gatttatggt ggactaattt tcatatattt cttattgctt ttaccttttc ttggtatgta 25200 agtccgtaac tggaattact gtgggttgcc atgacactct gtggtctttt ggttcatgca 25260 tggatcttgc gcaagaaaaa gacaaagaac aaagaaaaaa gacaaaacag agagacaaaa 25320 cgcaatcaca caaccaactc aaattagtca ctggctgatc aagatcgccg cgtccatgta 25380 tgtctaaatg ccatgcaaag caacacgtgc ttaacatgca ctttaaatgg ctcacccatc 25440 tcaacccaca cacaaacaca ttgccttttt cttcatcatc accacaacca cctgtatata 25500 ttcattctct tccgccacct caatttcttc acttcaacac acgtcaacct gcatatgcgt 25560 gtcatcccat gcccaaatct ccatgcatgt tccaaccacc ttctctctta tataatacct 25620 ataaatacct ctaatatcac tcacttcttt catcatccat ccatccagag tactactact 25680 ctactactat aataccccaa cccaactcat attcaatact actctaggat ccacaaccaa 25740 aaatgcttca gcacacttgg cttccgaagc ctcccaatct gaccctcttg tcagatgagg 25800 ttcatctctg gaggattcct cttgaccagc ctgagtcaca acttcaagac cttgctgcca 25860 ccctgagcag tgatgaattg gcgagggcaa acagattcta ctttccagaa cacagaaggc 25920 gtttcactgc tgggagaggc atcctcagat ccatcttggg tggatacttg ggagtggaac 25980 cgggtcaagt caagtttgat tatgagtccc gtgggaaacc gatccttggt gacagatttg 26040 ctgagagtgg actcctgttc aacttgtctc acagccagaa ccttgccttg tgtgctgtca 26100 actacacgcg tcaaataggc attgatcttg aatatctgcg tccaacatct gacttggagt 26160 ctcttgcaaa gaggttcttt ctcccaagag aatatgaact cttgaggtca ctccctgatg 26220 agcagaaaca gaagattttc tttcgttact ggacttgcaa agaggcttat ctcaaagcaa 26280 cgggagatgg aatagccaaa cttgaagaga tcgagatagc actcacccca acagaacctg 26340 ccaagctcca aacagctcct gcgtggtctc tgttggagct tgtgccagat gacaattgtg 26400 ttgcagctgt ggctgttgcg ggttttggtt ggcagcccaa gttctggcat tactgagtag 26460 ttagcttaat cacttaggtc acctttaaac ggtcgcagcg tgtgcgtgtc cgtcgtacgt 26520 tctggccggc cgggccttgg gcgcgcgatc agaagcgttg cgttggcgtg tgtgtgcttc 26580 tggtttgctt taattttacc aagtttgttt caaggtggat cgcgtggtca aggcccgtgt 26640 gctttaaaga cccaccggca ctggcagtga gtgttgctgc ttgtgtaggc tttggtacgt 26700 atgggcttta tttgcttctg gatgttgtgt actacttggg tttgttgaat tattatgagc 26760 agttgcgtat tgtaattcag ctgggctacc tggacattgt tatgtattaa taaatgcttt 26820 gctttcttct aaagatcttt aagtgctcgg ccggccttgg ccacggtggc cagatccact 26880 agaggcgcgc ctctagtgcg gccgcttaat taactactgt cactgaggcc gtagacgagt 26940 acggactgat ctaactagtg ctagcctcga ggtcgacacc cagctttctt gtacaaagtg 27000 gttgcggccg cttaattaaa tttaaattca attaatgcaa tcttgatttt caacaacgaa 27060 ggtaatggcg taaaagaaaa aatgtatgtt attgtattga tctttcatga tgttgaagcg 27120 tgccataata tgatgatgta taattaaaat attaactgtc gcattttatt gaaatggcac 27180 tgttatttca accatatctt tgattctgtt acatgacacg actgcaagaa gtaaataata 27240 gacgccgttg ttaaagaatt gctatcatat gtgcctaact agagggaatt tgagcgtcag 27300 acctaatcaa atattacaaa atatctcact ctgtcgccag caatggtgta atcagcgcag 27360 acaaatggcg taaagatcgc ggaaaaacct ccccgagtgg catgatagct gcctctgtat 27420 tgctgattta gtcagcctta tttgacttaa gggtgccctc gttagtgaca aattgctttc 27480 aaggagacag ccatgcccca cactttgttg aaaaacaaat tgcctttggg gagacggtaa 27540 agccagttgc tcttcaataa ggaatgtcga ggaggcaatg taaccgcctc tggtagtaca 27600 cttctctaat ccaaaaatca atttgtattc aagataccgc aaaaaactta tggtttaaac 27660 cctgcaggac tagtccagaa ggtaattatc caagatgtag catcaagaat ccaatgttta 27720 cgggaaaaac tatggaagta ttatgtaagc tcagcaagaa gcagatcaat atgcggcaca 27780 tatgcaacct atgttcaaaa atgaagaatg tacagataca agatcctata ctgccagaat 27840 acgaagaaga atacgtagaa attgaaaaag aagaaccagg cgaagaaaag aatcttgaag 27900 acgtaagcac tgacgacaac aatgaaaaga agaagataag gtcggtgatt gtgaaagaga 27960 catagaggac acatgtaagg tggaaaatgt aagggcggaa agtaacctta tcacaaagga 28020 atcttatccc ccactactta tccttttata tttttccgtg tcatttttgc ccttgagttt 28080 tcctatataa ggaaccaagt tcggcatttg tgaaaacaag aaaaaatttg gtgtaagcta 28140 ttttctttga agtactgagg atacaacttc agagaaattt gtaagtttgt aggtaccaga 28200 tctggatccc aaaccatgtc tccggagagg agaccagttg agattaggcc agctacagca 28260 gctgatatgg ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc tacagtgaac 28320 tttaggacag agccacaaac accacaagag tggattgatg atctagagag gttgcaagat 28380 agataccctt ggttggttgc tgaggttgag ggtgttgtgg ctggtattgc ttacgctggg 28440 ccctggaagg ctaggaacgc ttacgattgg acagttgaga gtactgttta cgtgtcacat 28500 aggcatcaaa ggttgggcct aggatctaca ttgtacacac atttgcttaa gtctatggag 28560 gcgcaaggtt ttaagtctgt ggttgctgtt ataggccttc caaacgatcc atctgttagg 28620 ttgcatgagg ctttgggata cacagcccgg ggtacattgc gcgcagctgg atacaagcat 28680 ggtggatggc atgatgttgg tttttggcaa agggattttg agttgccagc tcctccaagg 28740 ccagttaggc cagttaccca aatctgagta gttagcttaa tcacctagag ctcgatcggc 28800 ggcaatagct tcttagcgcc atcccgggtt gatcctatct gtgttgaaat agttgcggtg 28860 ggcaaggctc tctttcagaa agacaggcgg ccaaaggaac ccaaggtgag gtgggctatg 28920 gctctcagtt ccttgtggaa gcgcttggtc taaggtgcag aggtgttagc gggatgaagc 28980 aaaagtgtcc gattgtaaca agatatgttg atcctacgta aggatattaa agtatgtatt 29040 catcactaat ataatcagtg tattccaata tgtactacga tttccaatgt ctttattgtc 29100 gccgtatgta atcggcgtca caaaataatc cccggtgact ttcttttaat ccaggatgaa 29160 ataatatgtt attataattt ttgcgatttg gtccgttata ggaattgaag tgtgcttgag 29220 gtcggtcgcc accactccca tttcataatt ttacatgtat ttgaaaaata aaaatttatg 29280 gtattcaatt taaacacgta tacttgtaaa gaatgatatc ttgaaagaaa tatagtttaa 29340 atatttattg ataaaataac aagtcaggta ttatagtcca agcaaaaaca taaatttatt 29400 gatgcaagtt taaattcaga aatatttcaa taactgatta tatcagctgg tacattgccg 29460 tagatgaaag actgagtgcg atattatggt gtaatacata ggaattcgtt taaacgatct 29520 gcgtctaatt ttcggtccaa cttgcacagg aaagacgtcg accgcggtag ctcttgccca 29580 gcagactggg cttccagtcc tttcgctcga tcgggtccaa tgttgtcctc agctgtgaac 29640 cggaagcgga cgaccaacag tggaagaact gaaaggaacg agccgtctat accttgatga 29700 tcggcctctg gtgaagggta tcatcgcagc caagcaagct catgaaaggc tgatggggga 29760 ggtgtataat tatgaggccc acggcgggct tattctttag ggaggatcta tctcgttgct 29820 caagtgcatg gcgcaaagca gttattggag tgcggatttt cgttggcata ttattcgcca 29880 cgagttagca gacgaagaga ccttcatgaa cgtggccaag gccagagtta agcagatgtt 29940 acgccctgct gcaggccttt ctattatcca atagttggtt gatctttgga aagagcctcg 30000 gctgaggccc atactgaaag agatcgatgg atatcgatat gccatgttgt ttgctagcca 30060 gaaccagatc acatccgata tgctattgca gcttgacgca gatatggagg ataagttgat 30120 tcatgggatc gctcaggagt agctcatcca tgcacgccga caagaacaga aattccgtcg 30180 agttaacgca gccgcttacg acggattcga aggtcatcca ttcggaatgt attagtttgc 30240 accagctccg cgtcacacct gtcttcattt gaataagatg ttagcaattg tttttagctt 30300 tgtcttgttg tggcagggcg gcaagtgctt cagacatcat tctgttttca aattttatgc 30360 tggagaacag cttcttaatt cctttggaaa taatagactg cgtcttaaaa ttcagatgtc 30420 tggatataga tatgattgta aaataaccta tttaagtgtc atttagaaca taagttttat 30480 gaatgttctt ccattttcgt catcgaacga ataagagtaa atacaccttt tttaacatta 30540 caaataagtt cttatacgtt gtttatacac cgggaatcat ttccattatt ttcgcgcaaa 30600 agtcacggat attcgtgaaa gcgacataaa ctgcgaaatt tgcggggagt gtcttgagtt 30660 tgcctcgagg ctagcgcatg cacatagaca cacacatcat ctcattgatg cttggtaata 30720 attgtcatta gattgttttt atgcatagat gcactcgaaa tcagccaatt ttagacaagt 30780 atcaaacgga tgtgacttca gtacattaaa aacgtccgca atgtgttatt aagttgtcta 30840 agcgtcaatt tgatttacaa ttgaatatat cctgccccag ccagccaaca gctcgattta 30900 caattgaata tatcctgccg gccggcccac gcgtgtcgag gaattctgat ctggccccca 30960 tttggacgtg aatgtagaca cgtcgaaata aagatttccg aattagaata atttgtttat 31020 tgctttcgcc tataaatacg acggatcgta atttgtcgtt ttatcaaaat gtactttcat 31080 tttataataa cgctgcggac atctacattt ttgaattgaa aaaaaattgg taattactct 31140 ttctttttct ccatattgac catcatactc attgctgatc catgtagatt tcccggacat 31200 gaagccattt acaattgaat atatcctgcc gccgctgccg ctttgcaccc ggtggagctt 31260 gcatgttggt ttctacgcag aactgagccg gttaggcaga taatttccat tgagaactga 31320 gccatgtgca ccttcccccc aacacggtga gcgacggggc aacggagtga tccacatggg 31380 acttttaaac atcatccgtc ggatggcgtt gcgagagaag cagtcgatcc gtgagatcag 31440 ccgacgcacc gggcaggcgc gcaacacgat cgcaaagtat ttgaacgcag gtacaatcga 31500 gccgacgttc acgcggaacg accaagcaag cttggctgcc atttttgggg tgaggccgtt 31560 cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg 31620 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct 31680 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt 31740 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct 31800 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc 31860 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc 31920 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt 31980 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg 32040 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg 32100 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg 32160 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa 32220 ccagcccggt gagcgtcgga aagggtcgac ggatcttttc cgctgcataa ccctgcttcg 32280 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt 32340 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag 32400 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg 32460 cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg 32520 agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcaagg 32580 tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga 32640 gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc gtcgtggact 32700 atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg ggcggcctgc 32760 tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc acgatcctcg 32820 ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg atgggcgtgg 32880 tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg ggtgcgcgtg 32940 attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtattc 33000 gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc 33060 gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag 33120 gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat 33180 cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag 33240 gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc 33300 gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct 33360 ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag 33420 gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag 33480 cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg 33540 ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg 33600 tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg 33660 cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag 33720 gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg 33780 cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc 33840 tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag 33900 gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac 33960 ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc 34020 cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg 34080 aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat 34140 ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga 34200 tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg 34260 cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc 34320 tagggccttg tggggtcagt tccggctggg ggttcagcag ccagcgcttt actggcattt 34380 caggaacaag cgggcactgc tcgacgcact tgcttcgctc agtatcgctc gggacgcacg 34440 gcgcgctcta cgaactgccg ataaacagag gattaaaatt gacaattgtg attaaggctc 34500 agattcgacg gcttggagcg gccgacgtgc aggatttccg cgagatccga ttgtcggccc 34560 tgaagaaagc tccagagatg ttcgggtccg tttacgagca cgaggagaaa aagcccatgg 34620 aggcgttcgc tgaacggttg cgagatgccg tggcattcgg cgcctacatc gacggcgaga 34680 tcattgggct gtcggtcttc aaacaggagg acggccccaa ggacgctcac aaggcgcatc 34740 tgtccggcgt tttcgtggag cccgaacagc gaggccgagg ggtcgccggt atgctgctgc 34800 gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca acgggaatct 34860 ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg agcttgttgt 34920 ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct gtgcagccgc 34980 tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg atggcggtcc 35040 tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca aacgcagcgc 35100 tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg ttcggaaccg 35160 tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc tggcaactgg 35220 cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca atcccgatgc 35280 ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg ggtttaacct 35340 acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc ttactgggct 35400 ttctcagccc ccgagcgctt agtgggaatt tgtacccctt atcgaaccgg gagcacagga 35460 tgacgcctaa caattcattc aagccgacac cgcttcgcgg cgcggcttaa ttcaggagtt 35520 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 35580 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 35640 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 35700 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 35760 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 35820 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 35880 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 35940 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 36000 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 36060 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 36120 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 36180 ccagtatcag cccgtcatac ttgaagctag gcaggcttat cttggacaag aagatcgctt 36240 ggcctcgcgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg agatcaccaa 36300 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct 36360 taactcaagc gttagagagc tggggaagac tatgcgcgat ctgttgaagg tggttctaag 36420 cctcgtcttg cgatggcatt tcgatccatt cccattccgc gctcaagatg gcttcccctc 36480 ggcagttcat cagggctaaa tcaatctagc cgacttgtcc ggtgaaatgg gctgcactcc 36540 aacagaaaca atcaaacaaa catacacagc gacttattca cacgagctca 36590 <210> 68 <211> 35499 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB9147 <400> 68 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctaggt ggagtcatca cgcagactat 240 ctcagcatgt gcgtagcacg cggccgcagc ttatagtaaa gatttttttg ttaataaaat 300 gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta ataaatttat 360 ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat ggatataata 420 ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa aagatattat 480 aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca caccgctttt 540 gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc tgccacgtca 600 gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc tcctcttctc 660 actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc gcctcttcac 720 ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta taaatagaga 780 gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat agcaaaacgg 840 atccacaacc aaaaatggca gcgaggctgc aagagcagaa aggaggtgag atggacacga 900 ggattgcgat cataggcatg agcgccatac ttccttgtgg aaccacggtg agagagtcat 960 gggagaccat aagggctggc attgattgcc tgtctgatct tcctgaggat cgtgttgatg 1020 tgactgcgta ctttgaccca gtcaagacca caaaggacaa aatctactgc aagagaggag 1080 gtttcattcc tgagtatgac tttgatgcgc gtgagtttgg tctcaacatg ttccagatgg 1140 aagattctga tgcgaatcag accatctcac ttctcaaggt caaagaagca cttcaagatg 1200 ctgggattga tgcacttgga aaggagaaga aaaacatagg atgcgtgctt ggcattggag 1260 gtggacagaa gagttcacat gagttctaca gcagactcaa ctatgtggtt gtggagaaag 1320 tcttgagaaa gatggggatg ccagaggaag atgtcaaagt ggctgtggag aagtacaaag 1380 cgaacttccc agaatggaga cttgactctt tccctggatt ccttgggaat gtcactgctg 1440 gcagatgcac caacacattc aacttggatg gcatgaactg cgttgtggat gctgcatgtg 1500 caagctcctt gatagcagtc aaggttgcga ttgatgaact gctctatggt gactgcgaca 1560 tgatggtcac tggtgcaacc tgcacagaca actccattgg gatgtacatg gctttctcaa 1620 agacacctgt gttcagcacg gacccaagtg tgagagcgta tgatgagaaa acgaaaggga 1680 tgctcattgg tgagggatca gccatgttgg tgctcaaacg ttatgcagat gcagtgaggg 1740 atggtgatga gattcatgct gtgattcgtg gttgtgcaag ctcaagtgat ggcaaagcag 1800 ctggcatcta cactcccaca atcagtggtc aagaggaagc cttgagacgt gcatacaatc 1860 gtgcctgcgt tgaccctgcc actgtcactt tggttgaggg tcatgggact ggcactccag 1920 ttggagatag gattgagttg actgcgttga ggaacttgtt tgacaaagcg tatggtgaag 1980 ggaacacaga gaaagttgca gttggaagta tcaagtccag tattggtcat ttgaaggctg 2040 ttgctggtct tgctggaatg atcaaggtca tcatggctct caaacacaag actttgcctg 2100 gaacgatcaa tgtggacaat ccacccaatc tctatgacaa cactcccatc aatgagtcat 2160 ccctctacat caacacgatg aacagaccct ggttccctcc acctggtgtt ccgagacgtg 2220 ctggaatatc cagctttggc tttggaggtg ccaactacca tgcagtgttg gaagaggctg 2280 aaccagagca cacgacagcg tacagactca acaaacgtcc acaaccggtg ttgatgatgg 2340 ctgcaactcc tgctgcgttg cagagccttt gtgaagctca gctgaaagag tttgaagctg 2400 cgatcaagga gaatgagaca gtcaagaata ctgcctacat caagtgtgtc aagtttggag 2460 aacagttcaa gttccctggg agcatacctg cgaccaatgc gagactggga ttcttggtca 2520 aagatgcgga ggatgcgtgc tccacgcttc gtgctatatg tgctcagttt gccaaagatg 2580 tgaccaaaga agcgtggagg cttccgagag aaggagtttc tttccgtgcc aaaggcattg 2640 ccaccaatgg tgctgttgct gcactcttct ctggacaagg tgctcagtac acacacatgt 2700 tcagtgaagt tgcgatgaac tggcctcagt tcagacagtc cattgctgca atggacgctg 2760 cacagtccaa ggttgctgga agtgacaaag actttgaaag agtctctcaa gtcctgtacc 2820 caaggaaacc gtacgaacgt gagccagaac aagatcacaa gaaaatctca ctcacagcat 2880 acagccagcc gtcaaccctg gcatgtgctc ttggtgcctt tgagattttc aaggaagctg 2940 gattcactcc agactttgca gctggacaca gcttgggtga gtttgctgcg ctctatgcag 3000 ctggttgtgt tgacagagat gagttgtttg aacttgtttg cagaagggca aggatcatgg 3060 gaggcaaaga tgcacctgcc acacccaaag gttgcatggc agctgtcata ggacccaacg 3120 cagagaacat caaggttcaa gctgccaacg tctggcttgg aaactcaaac tctccgtctc 3180 agactgtcat tactgggtct gttgaaggga ttcaagctga aagtgccaga ttgcagaagg 3240 aagggttcag agttgtgcct cttgcttgtg agtctgcctt ccattctcca cagatggaaa 3300 atgccagctc agctttcaaa gatgtcatat ccaaggtttc cttccgtaca cccaaagcag 3360 aaacaaagct cttcagcaat gtttctggag agacctatcc gacggatgcc agagagatgc 3420 tcactcaaca catgacctca tctgtgaagt tcctgaccca agtgaggaac atgcaccaag 3480 ctggtgcgag aatctttgtt gagtttggac cgaaacaagt tctcagcaaa cttgtttctg 3540 aaaccctcaa agatgacccg tctgttgtca cagtgtctgt gaacccagct tctggcacgg 3600 actctgacat acagttgagg gatgctgcag ttcagctggt tgtggctggt gtcaatcttc 3660 aagggtttga caagtgggat gctcctgatg ccacgaggat gcaagccatc aagaaaaaga 3720 gaaccacact cagactttca gctgccacct atgtcagtga caagaccaag aaagtgaggg 3780 atgctgccat gaacgacgga agatgtgtca catacctcaa gggtgctgca ccactcatca 3840 aggctcctga acctgtggtt gacgaagctg ccaagaggga ggctgaaagg cttcagaagg 3900 agcttcaaga cgctcagagg cagcttgacg acgccaaacg tgcagctgca gaagccaact 3960 ccaaacttgc tgctgccaag gaggaagcca agactgctgc agcttcagcc aagcctgcag 4020 ttgacactgc tgttgtggag aaacatcgtg ccatcctcaa gtccatgctg gctgagctgg 4080 atggttatgg aagtgttgat gcttcttccc ttcagcaaca gcaacagcaa cagactgctc 4140 cggcgcctgt taaagccgct gcccctgccg ctcccgttgc aagtgctcct gctcccgccg 4200 tgtccaacga gttgcttgag aaagctgaga cagttgtgat ggaggttctc gctgccaaga 4260 ctggatacga aacagacatg attgaggcgg acatggagct tgagactgag ttgggaattg 4320 acagcatcaa gagggttgaa attctttctg aagtccaagc tatgcttaac gttgaggcga 4380 aagatgttga tgctctctct aggacgagaa cagtgggtga agttgtcaac gctatgaagg 4440 cagaaatcgc tggttcttcc gctccggcgc cggcagctgc cgcacctgca ccggctaaag 4500 cggctccagc tgcagctgca ccagctgttt ctaatgagct gctcgaaaag gcagaaactg 4560 ttgtgatgga agtccttgct gccaaaactg gctacgaaac tgacatgata gaatcagaca 4620 tggagcttga gacggagctt ggaattgatt ccatcaagcg tgtggaaatc ctcagtgaag 4680 tccaagccat gctcaatgtg gaagctaagg atgtggatgc tctctcaagg accagaactg 4740 tcggtgaggt tgtcaatgcc atgaaggctg agatagctgg aggctccgct cctgcgccgg 4800 ccgctgccgc tcctggtccc gctgccgcgg ctccagctcc agctgcagcc gctccggccg 4860 tctcaaacga actccttgaa aaagctgaga ccgtcgtgat ggaggtcttg gcagctaaaa 4920 ccggctatga gactgacatg attgagtctg acatggaatt ggagacagaa cttgggatag 4980 acagcatcaa acgtgttgaa atcctcagtg aggtccaagc tatgctgaac gtggaagcga 5040 aggatgttga tgctctgtcc agaacgagga ccgttggtga ggttgtggat gccatgaagg 5100 ctgaaattgc tggagggtct gcgcccgcgc cagctgcagc cgctcctgca cccgcagccg 5160 cggcacctgc tccagctgca cctgcacctg ccgtttcttc agaactcttg gagaaggctg 5220 agacggttgt gatggaggtt ttggctgcaa agactggcta tgagaccgac atgattgaat 5280 ctgatatgga attggagaca gagcttggga tcgattccat caaaagagtg gagatcctga 5340 gtgaagtcca agccatgctc aatgttgaag ctaaggatgt tgatgcactt tcaagaacaa 5400 ggacagtggg tgaggttgtg gatgccatga aggcggagat tgctggaggg agcgcgcctg 5460 cgccagctgc cgcggcacct gcaccggcag ccgcagctcc agctccagct gcaccagcac 5520 cggccgcgcc cgcgccagct gtttcatccg aactcctgga aaaggccgag acggttgtga 5580 tggaggtgct cgcagctaag actggttacg agaccgacat gattgaaagt gacatggagc 5640 ttgaaaccga gttgggaata gatagcatca aacgtgttga aatcttgtct gaggtccaag 5700 ctatgttgaa cgtggaggca aaagatgtcg atgcgctttc aagaaccaga acagtcggtg 5760 aggtcgtgga cgccatgaag gctgaaattg ccggaagctc tgctagtgcg ccagctgcag 5820 ccgcacctgc acctgctgcc gcggcacctg ctccagctgc agccgctcct gccgtcagca 5880 atgagttgct ggagaaagcc gaaacagttg tgatggaagt ccttgctgca aaaactggtt 5940 acgaaacgga catgattgag tcagacatgg aattggagac cgaacttgga atagattcca 6000 tcaagagagt ggagatcctc tctgaggtgc aagccatgct caacgttgag gctaaagatg 6060 ttgatgcact cagtaggaca agaactgtgg gggaagttgt cgatgcgatg aaggctgaga 6120 ttgctggagg ctctgctcct gcgccggcag ctgccgcacc tgcacccgca gccgcggcac 6180 cagctgtcag caacgaattg cttgagaagg ctgaaacagt tgtcatggaa gtgctggctg 6240 caaaaaccgg ctatgagact gacatgattg agtctgacat ggaacttgag acggaattgg 6300 gcattgacag catcaagcgt gttgagatcc tcagtgaagt ccaagcaatg ctcaatgtgg 6360 aggctaagga tgtggatgcc ctctcaagga ccagaacagt tggtgaggtc gttgatgcga 6420 tgaaggcgga gatcgctgga agttccgcgc ctgcgccagc tgcagccgct cctgcacccg 6480 cagccgcggc accggctcca gctgcagccg caccggccgt ctcctcagaa ctcctggaaa 6540 aggctgagac agttgtgatg gaagtccttg ctgcgaaaac tggctatgag accgacatga 6600 ttgaatctga catggaactg gagaccgagc ttggaattga ctccatcaaa cgtgttgaaa 6660 tcctctctga ggttcaagcg atgttgaatg tggaggccaa agatgttgat gctctttcca 6720 gaacaaggac ggtgggagag gtggttgatg ccatgaaggc tgagatagct gggggttctg 6780 ctcctgcgcc agctgcagcc gctcctgctc ccgctgccgc ggcaccagct gtcagcaacg 6840 aactccttga aaaagctgaa actgttgtga tggaggtttt ggctgcaaag actggatacg 6900 agactgacat gatagagtca gacatggagt tggaaacaga gcttgggatt gatagcatca 6960 agcgtgtgga aatcctttct gaggttcaag ccatgctgaa cgttgaggcc aaagatgtcg 7020 atgctttgtc aaggaccaga acggttggag aagtggtcga tgccatgaag gctgagatag 7080 ctggtggatc agcccctgct ccggctgcag ccgctcctgc aagtgccggt gctgcaccag 7140 ctgtgaagat tgattcagtt catggtgctg actgtgacga tttgtcactg atgcacgcaa 7200 aggtggttga catcagaagg ccagatgaac tgattctgga aagaccggag aaccgtccag 7260 ttcttgtcgt tgacgatggc tcagagttga cacttgctct tgtgagggtg ttgggagcct 7320 gtgcagttgt cttgaccttt gagggattgc agttggctca gagagctggt gcagctgcca 7380 ttcgtcatgt tctggctaag gacctttcag cagaatcagc tgagaaggcc atcaaagagg 7440 ctgagcaaag atttggagcg ttgggaggct tcataagcca gcaagcagaa aggtttgaac 7500 cagctgagat actgggtttc acactcatgt gtgcgaagtt tgccaaagcc tctctctgca 7560 ctgctgtggc tggaggtcgt cctgccttca tcggtgtggc taggcttgac ggcagacttg 7620 gattcacctc tcaaggcact tctgacgcac tcaagagagc acagcgtgga gcgatctttg 7680 gactctgcaa gacaataggg ttggagtggt cagaatctga tgtgttcagc cgtggtgtgg 7740 acattgctca agggatgcac ccagaagatg ctgcagttgc catagttagg gagatggcgt 7800 gtgctgacat cagaatcaga gaggttggga ttggagcaaa ccagcaaaga tgcacaatca 7860 gagctgccaa gttggagact gggaaccctc agagacagat agcgaaagac gatgtgcttc 7920 tggtttctgg aggtgctcgt gggatcacac ctctttgcat ccgtgagatc acgcgtcaga 7980 ttgctggagg caagtacatt ctgttgggtc gtagcaaagt ttctgcgtct gaaccagcat 8040 ggtgtgctgg catcactgat gagaaggctg tgcagaaagc tgccacccaa gagttgaaga 8100 gggccttcag tgctggtgaa ggacccaaac ccactccaag ggctgtcacg aaacttgtgg 8160 gatctgttct tggtgctcgt gaagtgagaa gctccattgc tgccattgaa gctctgggtg 8220 gcaaagcgat ctacagctct tgtgatgtga actctgctgc agatgttgcc aaagcagtta 8280 gggatgcaga gtcccagctt ggtgccagag ttagtggaat cgttcacgct tctggtgtgc 8340 tccgtgatcg tctcatagag aagaaactcc ctgacgagtt tgatgctgtc tttgggacca 8400 aagtcactgg gttggagaat cttctggctg cagttgatcg tgccaacctc aaacacatgg 8460 ttctcttcag ctcccttgct ggtttccacg gcaatgttgg tcagtctgac tatgcgatgg 8520 caaacgaagc tctcaacaag atgggtttgg aacttgcgaa ggatgtcagt gtcaagtcca 8580 tctgctttgg tccctgggat ggtgggatgg tgacacctca actcaagaaa cagtttcaag 8640 aaatgggagt tcagatcatt cctcgtgaag gaggtgctga tacagttgcg aggattgtgt 8700 tgggatcaag tcctgctgag atccttgttg gaaactggag gacaccatcc aagaaagttg 8760 gaagtgacac gatcaccctg catagaaaga tcagtgccaa gtccaatccc ttcttggagg 8820 atcatgtcat acaaggcaga agggtgttgc caatgaccct tgctattggg agccttgctg 8880 agacctgcct tggactcttc cctggctact ctctctgggc catagatgac gcacagctct 8940 tcaaaggtgt cactgttgat ggagacgtca actgtgaagt gaccctcaca ccgtccactg 9000 ctccgagtgg gagggtgaat gtccaagcga cgctcaagac gttctcaagt ggaaaacttg 9060 ttccagccta cagagctgtc atagtgttgt ccaaccaagg tgctccacct gccaatgcaa 9120 ccatgcagcc tccgagcctt gatgcagatc ctgctttgca agggtcagtc tatgatggca 9180 agactctctt tcatggtcct gcgttcagag gcattgatga cgttctcagc tgcaccaaga 9240 gccagcttgt tgccaagtgt tctgctgttc ctggatcaga tgcagccaga ggagagtttg 9300 caactgacac tgatgcacat gatccctttg tcaatgatct tgcctttcaa gcaatgttgg 9360 tctgggtgag gagaacgctg ggacaagctg cactcccgaa ctccattcag aggatagtcc 9420 aacacagacc ggtcccacaa gacaaaccct tctacatcac cctcagatcc aatcagagtg 9480 gtggacatag ccagcacaaa catgcacttc agttccacaa cgaacaaggt gatctcttca 9540 ttgatgttca agcctctgtc attgcgacag actcacttgc gttctgagta gttagcttaa 9600 tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg ttgctattga 9660 tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa gcttaggatg 9720 tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact ttatgtttta 9780 ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag ctaaactaaa 9840 gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga ctactactgc 9900 tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga gtaaatctgg 9960 cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa tctcaattgc 10020 ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa gaacgtcgta 10080 attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt ctttatatgc 10140 acttactgat gcggccgctt aattaagatg agtgatactc aggactcagg actcactctg 10200 ctgatcacta gtggtcgcag cgtgtgcgtg tccgtcgtac gttctggccg gccgggcctt 10260 gggcgcgcga tcagaagcgt tgcgttggcg tgtgtgtgct tctggtttgc tttaatttta 10320 ccaagtttgt ttcaaggtgg atcgcgtggt caaggcccgt gtgctttaaa gacccaccgg 10380 cactggcagt gagtgttgct gcttgtgtag gctttggtac gtatgggctt tatttgcttc 10440 tggatgttgt gtactacttg ggtttgttga attattatga gcagttgcgt attgtaattc 10500 agctgggcta cctggacatt gttatgtatt aataaatgct ttgctttctt ctaaagatct 10560 ttaagtgctg tcgacacaac tttgtataca aaagttgtct agacctaggc tcagcaactc 10620 atccagtcaa gttcgagtca gtgagttggg cggccgcagc ttatagtaaa gatttttttg 10680 ttaataaaat gaaagttaat atttatggat ttatttgaac atatttacaa gtttttgtta 10740 ataaatttat ttacagaagt gttaaatttg aaaatgtgtt aaatgagagt taatatttat 10800 ggatataata ttactttatt taaaaataaa atgaatttat attaaaatat gatttggtaa 10860 aagatattat aaagaggaac ttggatgaga aagacgacga tgggcaaaag ttgcatggca 10920 caccgctttt gttcagtaac atctgcactg tggcgccttt ttcacactca ccatgcatgc 10980 tgccacgtca gctttcacac tatgacacca ctggccatac acgttgccac ctcagctttc 11040 tcctcttctc actatgacac gactggccat gcatgcatgc atgctgccac ctcagctccc 11100 gcctcttcac ccgtgtcttt ctctcatgtc gcagtgctgc gtgccaacct cttctctcta 11160 taaatagaga gaagagaatg atggttaaac tcaaaccaaa acaagaaaac atacacaaat 11220 agcaaaacgg atccacaacc aaaaatggct gcaaggaacg tgagtgcggc tcatgagatg 11280 catgacgaga agaggatagc tgttgtgggc atggctgttc agtatgctgg atgcaaaacg 11340 aaagacgagt tctgggaagt tctcatgaat ggaaaagttg agagcaaagt gatcagcgac 11400 aaacgtcttg gttcaaatta ccgtgctgaa cactacaaag ctgaaaggag caagtatgct 11460 gacaccttct gcaatgagac atatggaact cttgatgaga acgaaataga caatgagcat 11520 gaactccttt tgaaccttgc caaacaagcc ttggctgaaa catctgtcaa agattcaaca 11580 agatgtggca tagtttctgg atgcctcagc ttcccgatgg acaacctcca aggtgaactc 11640 ttgaatgtgt accagaacca tgttgagaag aaacttggtg ccagagtttt caaagatgcc 11700 tctcactggt cagaaagaga acagtccaac aaaccagagg ctggtgacag aagaatcttc 11760 atggaccctg cttcctttgt tgctgaggaa ctcaatcttg gcgctctcca ctactctgtt 11820 gatgctgcct gtgccactgc gctctatgtg ctcagactgg cgcaagatca cttggtctct 11880 ggtgcagctg atgtgatgct ttgtggtgca acatgcctgc cggagccgtt tttcatactc 11940 tctgggttct caaccttcca agccatgcct gttggaacgg gtcagaatgt gtccatgccg 12000 ctgcacaaag attctcaagg attgacccct ggtgaaggag gttccatcat ggtcctcaaa 12060 cgtttggatg acgcaatcag agatggagat cacatctatg gaacactttt gggagccaat 12120 gtcagcaaca gtggcactgg tctgccactt aaaccccttt tgccgtcaga gaaaaagtgc 12180 ctcatggaca cctacacacg tatcaatgtg catccccaca agattcagta tgttgaatgc 12240 catgcaactg ggactccaca aggtgatcgt gtggaaattg atgctgtgaa ggcttgcttt 12300 gaaggcaaag tcccacgttt tggaaccaca aaagggaact ttggacacac attggtggca 12360 gctgggtttg ctggcatgtg caaagtgctt ctctccatga agcacgggat cattccaccc 12420 actccgggaa tagatgacga aacgaagatg gaccctctgg ttgtctctgg tgaggccatt 12480 ccctggccag aaacgaatgg agaaccgaag cgtgctggtc tctcagcgtt tggatttggt 12540 gggaccaatg ctcatgctgt ctttgaggaa catgacccaa gcaatgctgc atgcactggc 12600 catgactcca tttctgctct ctctgcacgt tgtggtggtg agtcaaacat gagaatagca 12660 atcactggga tggatgccac ttttggtgct ttgaaaggat tggatgcttt tgaaagggcc 12720 atctacactg gtgctcatgg tgctatacca ctcccagaaa agagatggag attcctgggg 12780 aaagacaagg acttccttga tctttgtgga gtcaaagcaa caccgcatgg ctgttacatt 12840 gaagatgttg aagttgattt ccagaggctg aggactccaa tgactcctga agatatgctc 12900 ttgcctcaac agcttctggc tgtcaccaca attgataggg ccatattgga ctctggcatg 12960 aagaaaggtg gaaatgtggc tgtctttgtg gggttgggga ccgatcttga actctaccgt 13020 caccgtgcaa gggttgctct caaggagaga gtgaggcctg aagccagcaa gaaactcaat 13080 gacatgatgc agtacatcaa tgattgtggg acaagcactt cttacaccag ctacataggc 13140 aacttggttg caacaagggt cagttcacaa tggggcttca cgggaccttc cttcacaatc 13200 acagaaggca acaattctgt gtacagatgt gctgaacttg gcaaatacct cttggaaacg 13260 ggagaggttg atggggttgt ggttgcgggt gttgatctct gtgggagtgc tgagaacctt 13320 tacgtcaagt caaggcgttt caaggtgtct acatctgaca ccccgagagc cagctttgat 13380 gcagctgctg atggttactt tgttggagag ggctgtggtg catttgtgct caagcgtgag 13440 acaagctgca ccaaggatga cagaatctat gcttgcatgg atgccatagt tcctggaaat 13500 gttccatctg catgtttgag agaggcattg gatcaagcca gagtcaagcc tggagacatc 13560 gagatgttgg agctttctgc tgattcagcg aggcatttga aggacccgtc tgttctccca 13620 aaggaactca ctgctgagga agagattggc ggacttcaga cgattcttcg tgacgatgac 13680 aagctcccaa ggaatgttgc aactggaagt gtcaaggcca ctgttggtga cactggttat 13740 gcttctggtg ctgccagttt gattaaggct gcgctttgca tctacaaccg ttacctgccc 13800 agcaatggag atgactggga tgaacctgca cctgaagcac cctgggacag cacactcttt 13860 gcgtgccaga cctcaagagc ttggttgaag aaccctggtg agagacgtta tgcggctgtt 13920 tccggagtca gtgaaaccag aagttgctac agtgtgctgc tttctgaagc agagggacac 13980 tacgagagag agaacagaat cagtttggat gaggaagcac ccaagctcat tgtgttgaga 14040 gcagactccc atgaagagat tcttggaaga ttggacaaga taagggaaag gttcttgcag 14100 cccacgggtg ctgcacccag agagtctgaa ttgaaggcgc aagcccgtag gatctttctt 14160 gaactccttg gtgagaccct tgcccaagat gcagcttcat ctggaagtca gaagcccctt 14220 gctctcagct tggtctccac tccatccaag ctccagagag aggtggaact tgcggcaaaa 14280 ggcattccga gatgtctcaa gatgagacgt gattggagtt cccctgctgg aagcagatat 14340 gcgcctgagc ctttggccag tgacagagtt gccttcatgt atggagaagg aaggagccct 14400 tactatggca tcacacaaga cattcatagg atttggcctg aacttcatga ggtcatcaat 14460 gagaaaacaa accgtctctg ggctgaaggt gacagatggg tcatgccaag ggccagcttc 14520 aagagtgagc ttgagtcaca acagcaagag tttgacagaa acatgattga aatgttccgt 14580 cttggaatcc tcacctcaat tgccttcaca aacctggcga gagatgttct caacatcacg 14640 cccaaagcag cctttggact cagtttggga gagatttcca tgatctttgc tttctccaag 14700 aaaaacggtt tgataagcga tcagttgaca aaggatttga gagagtcaga tgtctggaac 14760 aaagctctgg ctgttgagtt caatgcgctc agagaagcct ggggcattcc acagtctgtc 14820 ccaaaagatg agttctggca aggttacata gtgaggggaa cgaaacaaga catagaagca 14880 gccatagcac cagactcaaa gtatgtgagg ttgaccatca tcaatgatgc caacactgct 14940 ctcatcagtg gaaagcctga tgcatgcaaa gctgcgattg ctcgtcttgg tggcaacatc 15000 cctgcgcttc cagtcactca aggcatgtgt gggcattgcc cagaggtggg accttacacc 15060 aaggacatcg caaagatcca cgccaacctt gagttccctg tggttgatgg tcttgatctc 15120 tggactacca taaaccagaa gaggcttgtg ccaagagcca cgggtgccaa agatgaatgg 15180 gctccttcat cctttgggga gtatgctgga cagctctatg agaaacaagc caacttccct 15240 cagatagttg aaaccatcta caagcagaac tatgatgtgt ttgttgaagt tgggccaaac 15300 aatcacagaa gcacagcagt gagaaccacg cttggaccac agagaaacca ccttgctggt 15360 gccatagaca agcagaacga ggatgcttgg acgacaatag tcaagctcgt ggcttcattg 15420 aaggctcacc ttgttcctgg agtgaccatc agtccgctct accactccaa gctcgttgca 15480 gaggctgagg cttgttatgc tgcgctctgt aaaggtgaga aacccaagaa aaacaagttt 15540 gtgagaaaga ttcagttgaa tggcagattc aacagcaagg cagaccccat ctcctcagca 15600 gaccttgcat ccttcccacc tgcggaccct gcgattgaag ctgccatctc ttcaaggatc 15660 atgaagcctg tggctccgaa attctatgcg cgtctcaaca ttgatgagca agatgagacc 15720 agagatccga tcttgaacaa ggacaatgcg ccttccagct ccagctcttc cagcagttcc 15780 tcttccagca gttcatctcc gtctcctgct ccttcagctc cagttcagaa gaaagctgcc 15840 ccagctgcgg agacaaaagc tgttgccagt gctgatgcac ttcgtagtgc cctgcttgat 15900 cttgactcca tgcttgcgct cagctctgca agtgcttctg gcaaccttgt tgagactgcg 15960 ccatctgatg ccagtgtcat tgtccctccg tgcaacattg ctgatcttgg cagcagagct 16020 ttcatgaaaa cttatggtgt ttctgcgcct ctgtacaccg gtgcaatggc caagggaatt 16080 gcctctgcgg acttggtgat cgcagctgga aggcaaggaa tccttgcgtc atttggtgct 16140 ggaggccttc caatgcaagt tgtgcgtgag tccattgaaa agattcaagc tgccctgccc 16200 aatggaccat atgctgtcaa cctcatccat tctccttttg acagcaacct tgagaaggga 16260 aatgttgatc tcttcttgga aaagggtgtc acttttgttg aggcttcagc cttcatgacg 16320 ttgacaccgc aagttgtcag atacagagcg gctgggttga cgaggaatgc agatgggtca 16380 gtcaacataa ggaacagaat cattgggaaa gtctccagaa ctgagctggc tgaaatgttc 16440 atgcgtcctg cacctgagca tcttttgcag aagctcattg cttctgggga gatcaatcaa 16500 gaacaagctg agttggccag acgtgttcct gtggctgatg acattgcggt tgaagctgat 16560 tctggtggac acactgacaa ccgtccaatc catgtcattc tgcctctgat tatcaacctg 16620 agggataggc ttcacagaga gtgtggatac ccagcaaacc tgagggttcg tgttggtgct 16680 gggggtggca ttgggtgtcc ccaagctgcg ctggcaactt tcaacatggg tgcctctttc 16740 attgtcactg gaacagtgaa ccaagttgcc aaacaatctg ggacgtgtga caatgtgagg 16800 aaacagcttg cgaaagcaac ttactcagat gtttgcatgg caccggcagc tgacatgttt 16860 gaggaaggtg tgaaacttca agttttgaag aaaggaacaa tgttcccttc aagagccaac 16920 aaactctatg agttgttctg caagtatgat tcttttgagt ccatgcctcc agcagagctt 16980 gcgagggttg agaaaagaat cttcagtcgt gcattggaag aggtctggga tgagaccaag 17040 aacttctaca tcaaccgtct ccacaaccca gagaagatac agagggctga gagggacccc 17100 aaactgaaga tgtcactgtg tttcagatgg tatctgtccc tggcttcaag gtgggccaac 17160 actggagctt ctgacagagt catggactac caagtttggt gtggtcctgc cattggttca 17220 ttcaatgatt tcatcaaagg aacttacctt gatccggcag tggcaaatga gtacccgtgt 17280 gttgtgcaga tcaacaaaca gattcttaga ggagcgtgct tcttgaggcg tcttgaaata 17340 ctgagaaatg caaggctttc agatggtgca gctgcacttg tggcgagcat tgatgacaca 17400 tatgtccctg ctgagaagct gtgagtagtt agcttaatca cttaggtcac ccagtccaag 17460 cccggcctag cacgatgatc atgagtgttg ctattgatgt atgttaatac tatacacagt 17520 catcgtgtgt gttttataaa actgtaagct taggatgttt gaggctaatg taattagcac 17580 tacaccgtaa taaatgagag gtttacttta tgttttaata atgttcttac ccttgctgct 17640 ctctaaactg taatggcgta cggttagcta aactaaagta aaaatcgatt cgcttgagta 17700 aaattagtga ccgggaaaga tcatcgacta ctactgctca gagatgtaat cggtaattaa 17760 gacttcaagc tcaataatta attaggagta aatctggctt cgaattttta aattgtcaac 17820 ttccaaagaa ggattggcaa gactcaatct caattgccca acaaacatat ggctctttac 17880 aaatttggaa tctctttttg atggcaagaa cgtcgtaatt cctctgtcga tctcatgtaa 17940 ttaccaaact catctactta ttactttctt tatatgcact tactgatgcg gccgcttaat 18000 taatctgacc tgaccactag acctgagtga gtacagatgt gctactagtg ctagcctcga 18060 ggtcgaccaa cttttctata caaagttgtc tagacctagg actaggcact gagtgactca 18120 ctgacctgac tgagtgactg gcggccgcag cttatagtaa agattttttt gttaataaaa 18180 tgaaagttaa tatttatgga tttatttgaa catatttaca agtttttgtt aataaattta 18240 tttacagaag tgttaaattt gaaaatgtgt taaatgagag ttaatattta tggatataat 18300 attactttat ttaaaaataa aatgaattta tattaaaata tgatttggta aaagatatta 18360 taaagaggaa cttggatgag aaagacgacg atgggcaaaa gttgcatggc acaccgcttt 18420 tgttcagtaa catctgcact gtggcgcctt tttcacactc accatgcatg ctgccacgtc 18480 agctttcaca ctatgacacc actggccata cacgttgcca cctcagcttt ctcctcttct 18540 cactatgaca cgactggcca tgcatgcatg catgctgcca cctcagctcc cgcctcttca 18600 cccgtgtctt tctctcatgt cgcagtgctg cgtgccaacc tcttctctct ataaatagag 18660 agaagagaat gatggttaaa ctcaaaccaa aacaagaaaa catacacaaa tagcaaaacg 18720 gatccacaac caaaaatggc gcttcgtgtc aagaccaaca agaagccatg ttgggagatg 18780 accaaggagg aactgacctc tggcaaaact gaggtgttca actatgagga actcctggag 18840 tttgcagagg gtgacatagc taaggttttt ggtccggagt ttgctgtcat agacaagtac 18900 cccagaaggg tgaggctgcc tgctcgtgaa tacctgcttg tgacaagggt cacactcatg 18960 gatgccgagg tcaacaacta cagagttggt gccagaatgg tcactgagta tgatcttcca 19020 gtgaatggcg aattgtctga gggaggtgat tgtccctggg cagttctggt tgagagtgga 19080 cagtgcgatt tgatgctgat atcatacatg ggaattgatt tccagaacca aggtgacaga 19140 gtttaccgtc tgctcaacac cacgttgaca ttctatgggg ttgcccatga gggagagacc 19200 cttgagtatg acatcagagt gaccggtttt gccaaacgtt tggatggtgg catctccatg 19260 ttcttctttg agtatgactg ctatgtcaat gggaggcttc tcattgagat gagagatggc 19320 tgtgctggct tcttcacaaa tgaggagctt gatgcgggaa agggtgtggt gttcacaaga 19380 ggggatttgg ctgccagagc taagattcca aagcaagatg tttctcccta tgctgtcgct 19440 ccctgccttc acaaaacaaa actcaacgaa aaggagatgc agaccttggt tgacaaagat 19500 tgggcttccg tttttggctc aaagaatggc atgccggaga tcaactacaa actctgcgca 19560 cgtaagatgc tcatgattga tagagtcacc agcattgacc ataagggagg tgtgtacggt 19620 ttgggtcagt tggttggtga gaaaatcttg gagagggacc attggtactt cccatgccat 19680 tttgttaagg atcaagtcat ggctggatct cttgtcagcg acggttgttc acaaatgctc 19740 aagatgtaca tgatctggct cggtttgcac cttaccaccg gaccatttga cttcagaccc 19800 gtcaacggac acccgaacaa agttaggtgt aggggtcaga tatctcctca taagggaaag 19860 ttggtctacg tcatggagat caaagagatg ggattcgacg aggataacga tccgtacgcc 19920 attgctgacg tcaacatcat tgacgttgat ttcgaaaagg gtcaagattt cagccttgat 19980 agaatcagcg actacggaaa gggggatctt aacaaaaaga tcgtcgtgga cttcaagggc 20040 attgctctca agatgcagaa gagatccacg aacaagaacc cctccaaagt tcagcccgtt 20100 tttgctaacg gtgcagccac tgtgggaccg gaagcgtcca aggccagctc tggtgccagt 20160 gctagtgctt ctgctgcccc tgcgaagcct gctttctcag cagatgttct tgcgccaaag 20220 ccagttgcac ttcctgaaca tatcctcaag ggtgacgctc tcgctccgaa agaaatgtcc 20280 tggcatccca tgtcaaaact tcctggcaac ccgacgccgt tcttctcccc tagttcttac 20340 cctccgaggg caatttgctt catccctttc cctggcaacc cacttgacaa caattgcaaa 20400 gctggagaaa tgcctctgaa ctggtacaac atgtcagagt tcatgtgtgg aaaagttagc 20460 aactgcttgg gaccagagtt cgcaagattt gataagtcaa atacgagcag aagccctgct 20520 tttgatttgg ctcttgtgac tagagttgtt gaagtcacaa acatggaaca tggcaagttc 20580 ctgaatgttg attgcaatcc cagcaaaggc acaatggtgg gggagtttga ctgtcctcaa 20640 gatgcgtggt tctttgatgg ttcttgcaat gatgggcaca tgccttattc catcatcatg 20700 gaaatcggac ttcagacatc cggtgttctc accagtgtgt tgaaagcacc actgacgatg 20760 gacaaagatg acattctctt taggaacctt gatgcaagtg ctgaaatggt gaggccagat 20820 gtggatgtga gggggaaaac cataaggaat gtgaccaagt gcactggata tgcaatgttg 20880 ggaaagatgg ggattcacag attcacgttt gagttgtctg ttgatggcgt ggttttctac 20940 aaaggaagca cttcctttgg atggttcact ccagaggtgt ttgctcagca agctggactt 21000 gacaatggga aaaagacgga gccttggtgc aaaactaaca acacgagtgt gagaagagtt 21060 gaaatcgcat cagccaaagg caaagaacag ctgactgaga aacttccaga tgcaactaat 21120 gctcaagttc tcagaaggtc agaacagtgt gagtatttgg attacctcaa cattgctcct 21180 gattctgggc tgcatgggaa aggatatgcg catggacaca aagatgttaa cccacaagac 21240 tggttcttct cttgtcactt ttggtttgat cctgtgatgc ctggatcttt gggaattgag 21300 tcaatgttcc agctcattga ggcatttgcg gtggaccaga acataccggg agaatacaat 21360 gtgagcaatc cgacctttgc ccatgcccct ggaaaaacgg cgtggaagta ccgtggtcaa 21420 ttgacaccaa agaaccgtgc gatggactgt gaggtgcaca tagtttcaat cacagcctct 21480 cctgaaaatg gaggctacgt tgacatagtg gcagatggag cactttgggt tgatggtctc 21540 cgtgtgtatg aagccaaaga actcagagtt cgtgttgtca gtgcaaaacc tcaagccatt 21600 ccggatgttc aacaacagcc acccagcgca aaagcggacc ctgggaaaac tggagttgca 21660 ctcagtccga ctcagttgag agatgtcctg cttgaagtgg acaatccatt gtatcttggt 21720 gttgaaaact ccaatttggt gcagtttgaa tccaaacctg caacttcttc aaggattgtc 21780 agtatcaaac catgctccat aagtgatctt ggtgacaagt ccttcatgga aacgtacaat 21840 gtgtcagcac ctctgtacac tggagcaatg gccaagggaa ttgcgtctgc ggaccttgtc 21900 attgctgctg ggaagaggaa gatattgggt tcttttggag cgggtgggct tccaatgcac 21960 catgtgaggg cagctttgga gaagatacaa gcggctctgc ctcaaggacc atatgctgtg 22020 aacctcattc attcaccttt tgacagcaac ttggaaaagg ggaatgttga tctcttcctc 22080 gaaaagggcg ttactgtggt ggaggcttct gcattcatga ccctcactcc ccaagttgtt 22140 cgttaccgtg cagccggact ctcaaggaac gctgatggga gtgtcaacat cagaaaccgt 22200 atcattggaa aagtgtccag aacagaactc gccgaaatgt tcatcagacc ggctccggaa 22260 cacctccttg agaaactcat agcttctggt gagataacac aagaacaagc cgaacttgcc 22320 cgtcgtgttc ctgtcgcaga cgacatcgct gttgaagctg attctggtgg tcacactgac 22380 aaccgtccca tccacgtcat cttgcctttg atcatcaacc ttaggaacag actgcacaga 22440 gagtgtggtt atcctgcgca ccttagagtc agagttggag ctggaggcgg tgttggctgc 22500 ccacaagctg ctgcggctgc cctcacaatg ggagctgcct tcatagtgac tgggactgtc 22560 aatcaagttg ctaaacagtc cgggacatgt gacaacgtga gaaaacaact cagtcaagcc 22620 acctacagtg acatttgcat ggctccagca gctgatatgt ttgaggaagg tgtgaaactc 22680 caagttctca agaagggaac aatgttccca tcaagagcta acaaattgta cgaactcttt 22740 tgcaagtacg actctttcga ttcaatgcct cctgctgaac ttgaaaggat cgagaagagg 22800 atcttcaaga gggcacttca agaggtctgg gaagagacca aggactttta catcaatggt 22860 ttgaagaatc cagagaagat acagagagcg gaacatgacc ccaaactcaa gatgtctttg 22920 tgtttcagat ggtatcttgg tcttgcatca cgttgggcca acatgggtgc tcctgatcgt 22980 gtcatggact atcaagtctg gtgtggtcca gccattggag ccttcaatga cttcatcaaa 23040 ggaacatatc ttgatcctgc tgtctctaat gagtacccat gtgttgtcca gatcaacctt 23100 cagattttgc gtggtgcatg ttacctgagg cgtcttaatg ctctgagaaa tgatccgagg 23160 attgatcttg agactgaaga tgctgccttt gtctatgagc caacgaatgc gctttgagta 23220 gttagcttaa tcacttaggt cacccagtcc aagcccggcc tagcacgatg atcatgagtg 23280 ttgctattga tgtatgttaa tactatacac agtcatcgtg tgtgttttat aaaactgtaa 23340 gcttaggatg tttgaggcta atgtaattag cactacaccg taataaatga gaggtttact 23400 ttatgtttta ataatgttct tacccttgct gctctctaaa ctgtaatggc gtacggttag 23460 ctaaactaaa gtaaaaatcg attcgcttga gtaaaattag tgaccgggaa agatcatcga 23520 ctactactgc tcagagatgt aatcggtaat taagacttca agctcaataa ttaattagga 23580 gtaaatctgg cttcgaattt ttaaattgtc aacttccaaa gaaggattgg caagactcaa 23640 tctcaattgc ccaacaaaca tatggctctt tacaaatttg gaatctcttt ttgatggcaa 23700 gaacgtcgta attcctctgt cgatctcatg taattaccaa actcatctac ttattacttt 23760 ctttatatgc acttactgat gcggccgctt aattaatcac gagtggatga ctgtcacgac 23820 tgactcatga ctgactacta gtgctagcct cgaggtcgac acaactttgt ataataaagt 23880 tgtctagacc taggagctgt gctgggtaac tcacatgagt cactggtcac atcagcggcc 23940 gcagcttata gtaaagattt ttttgttaat aaaatgaaag ttaatattta tggatttatt 24000 tgaacatatt tacaagtttt tgttaataaa tttatttaca gaagtgttaa atttgaaaat 24060 gtgttaaatg agagttaata tttatggata taatattact ttatttaaaa ataaaatgaa 24120 tttatattaa aatatgattt ggtaaaagat attataaaga ggaacttgga tgagaaagac 24180 gacgatgggc aaaagttgca tggcacaccg cttttgttca gtaacatctg cactgtggcg 24240 cctttttcac actcaccatg catgctgcca cgtcagcttt cacactatga caccactggc 24300 catacacgtt gccacctcag ctttctcctc ttctcactat gacacgactg gccatgcatg 24360 catgcatgct gccacctcag ctcccgcctc ttcacccgtg tctttctctc atgtcgcagt 24420 gctgcgtgcc aacctcttct ctctataaat agagagaaga gaatgatggt taaactcaaa 24480 ccaaaacaag aaaacataca caaatagcaa aacggatcca caaccaaaaa tgcttcagca 24540 cacttggctt ccgaagcctc ccaatctgac cctcttgtca gatgaggttc atctctggag 24600 gattcctctt gaccagcctg agtcacaact tcaagacctt gctgccaccc tgagcagtga 24660 tgaattggcg agggcaaaca gattctactt tccagaacac agaaggcgtt tcactgctgg 24720 gagaggcatc ctcagatcca tcttgggtgg atacttggga gtggaaccgg gtcaagtcaa 24780 gtttgattat gagtcccgtg ggaaaccgat ccttggtgac agatttgctg agagtggact 24840 cctgttcaac ttgtctcaca gccagaacct tgccttgtgt gctgtcaact acacgcgtca 24900 aataggcatt gatcttgaat atctgcgtcc aacatctgac ttggagtctc ttgcaaagag 24960 gttctttctc ccaagagaat atgaactctt gaggtcactc cctgatgagc agaaacagaa 25020 gattttcttt cgttactgga cttgcaaaga ggcttatctc aaagcaacgg gagatggaat 25080 agccaaactt gaagagatcg agatagcact caccccaaca gaacctgcca agctccaaac 25140 agctcctgcg tggtctctgt tggagcttgt gccagatgac aattgtgttg cagctgtggc 25200 tgttgcgggt tttggttggc agcccaagtt ctggcattac tgagtagtta gcttaatcac 25260 ttaggtcacc cagtccaagc ccggcctagc acgatgatca tgagtgttgc tattgatgta 25320 tgttaatact atacacagtc atcgtgtgtg ttttataaaa ctgtaagctt aggatgtttg 25380 aggctaatgt aattagcact acaccgtaat aaatgagagg tttactttat gttttaataa 25440 tgttcttacc cttgctgctc tctaaactgt aatggcgtac ggttagctaa actaaagtaa 25500 aaatcgattc gcttgagtaa aattagtgac cgggaaagat catcgactac tactgctcag 25560 agatgtaatc ggtaattaag acttcaagct caataattaa ttaggagtaa atctggcttc 25620 gaatttttaa attgtcaact tccaaagaag gattggcaag actcaatctc aattgcccaa 25680 caaacatatg gctctttaca aatttggaat ctctttttga tggcaagaac gtcgtaattc 25740 ctctgtcgat ctcatgtaat taccaaactc atctacttat tactttcttt atatgcactt 25800 actgatgcgg ccgcttaatt aactactgtc actgaggccg tagacgagta cggactgatc 25860 taactagtgc tagcctcgag gtcgacaccc agctttcttg tacaaagtgg ttgcggccgc 25920 ttaattaaat ttaaattcaa ttaatgcaat cttgattttc aacaacgaag gtaatggcgt 25980 aaaagaaaaa atgtatgtta ttgtattgat ctttcatgat gttgaagcgt gccataatat 26040 gatgatgtat aattaaaata ttaactgtcg cattttattg aaatggcact gttatttcaa 26100 ccatatcttt gattctgtta catgacacga ctgcaagaag taaataatag acgccgttgt 26160 taaagaattg ctatcatatg tgcctaacta gagggaattt gagcgtcaga cctaatcaaa 26220 tattacaaaa tatctcactc tgtcgccagc aatggtgtaa tcagcgcaga caaatggcgt 26280 aaagatcgcg gaaaaacctc cccgagtggc atgatagctg cctctgtatt gctgatttag 26340 tcagccttat ttgacttaag ggtgccctcg ttagtgacaa attgctttca aggagacagc 26400 catgccccac actttgttga aaaacaaatt gcctttgggg agacggtaaa gccagttgct 26460 cttcaataag gaatgtcgag gaggcaatgt aaccgcctct ggtagtacac ttctctaatc 26520 caaaaatcaa tttgtattca agataccgca aaaaacttat ggtttaaacc ctgcaggact 26580 agtccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac gggaaaaact 26640 atggaagtat tatgtaagct cagcaagaag cagatcaata tgcggcacat atgcaaccta 26700 tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata cgaagaagaa 26760 tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga cgtaagcact 26820 gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac atagaggaca 26880 catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa tcttatcccc 26940 cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt cctatataag 27000 gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat tttctttgaa 27060 gtactgagga tacaacttca gagaaatttg taagtttgta ggtaccagat ctggatccca 27120 aaccatgtct ccggagagga gaccagttga gattaggcca gctacagcag ctgatatggc 27180 cgcggtttgt gatatcgtta accattacat tgagacgtct acagtgaact ttaggacaga 27240 gccacaaaca ccacaagagt ggattgatga tctagagagg ttgcaagata gatacccttg 27300 gttggttgct gaggttgagg gtgttgtggc tggtattgct tacgctgggc cctggaaggc 27360 taggaacgct tacgattgga cagttgagag tactgtttac gtgtcacata ggcatcaaag 27420 gttgggccta ggatctacat tgtacacaca tttgcttaag tctatggagg cgcaaggttt 27480 taagtctgtg gttgctgtta taggccttcc aaacgatcca tctgttaggt tgcatgaggc 27540 tttgggatac acagcccggg gtacattgcg cgcagctgga tacaagcatg gtggatggca 27600 tgatgttggt ttttggcaaa gggattttga gttgccagct cctccaaggc cagttaggcc 27660 agttacccaa atctgagtag ttagcttaat cacctagagc tcgatcggcg gcaatagctt 27720 cttagcgcca tcccgggttg atcctatctg tgttgaaata gttgcggtgg gcaaggctct 27780 ctttcagaaa gacaggcggc caaaggaacc caaggtgagg tgggctatgg ctctcagttc 27840 cttgtggaag cgcttggtct aaggtgcaga ggtgttagcg ggatgaagca aaagtgtccg 27900 attgtaacaa gatatgttga tcctacgtaa ggatattaaa gtatgtattc atcactaata 27960 taatcagtgt attccaatat gtactacgat ttccaatgtc tttattgtcg ccgtatgtaa 28020 tcggcgtcac aaaataatcc ccggtgactt tcttttaatc caggatgaaa taatatgtta 28080 ttataatttt tgcgatttgg tccgttatag gaattgaagt gtgcttgagg tcggtcgcca 28140 ccactcccat ttcataattt tacatgtatt tgaaaaataa aaatttatgg tattcaattt 28200 aaacacgtat acttgtaaag aatgatatct tgaaagaaat atagtttaaa tatttattga 28260 taaaataaca agtcaggtat tatagtccaa gcaaaaacat aaatttattg atgcaagttt 28320 aaattcagaa atatttcaat aactgattat atcagctggt acattgccgt agatgaaaga 28380 ctgagtgcga tattatggtg taatacatag gaattcgttt aaacgatctg cgtctaattt 28440 tcggtccaac ttgcacagga aagacgtcga ccgcggtagc tcttgcccag cagactgggc 28500 ttccagtcct ttcgctcgat cgggtccaat gttgtcctca gctgtgaacc ggaagcggac 28560 gaccaacagt ggaagaactg aaaggaacga gccgtctata ccttgatgat cggcctctgg 28620 tgaagggtat catcgcagcc aagcaagctc atgaaaggct gatgggggag gtgtataatt 28680 atgaggccca cggcgggctt attctttagg gaggatctat ctcgttgctc aagtgcatgg 28740 cgcaaagcag ttattggagt gcggattttc gttggcatat tattcgccac gagttagcag 28800 acgaagagac cttcatgaac gtggccaagg ccagagttaa gcagatgtta cgccctgctg 28860 caggcctttc tattatccaa tagttggttg atctttggaa agagcctcgg ctgaggccca 28920 tactgaaaga gatcgatgga tatcgatatg ccatgttgtt tgctagccag aaccagatca 28980 catccgatat gctattgcag cttgacgcag atatggagga taagttgatt catgggatcg 29040 ctcaggagta gctcatccat gcacgccgac aagaacagaa attccgtcga gttaacgcag 29100 ccgcttacga cggattcgaa ggtcatccat tcggaatgta ttagtttgca ccagctccgc 29160 gtcacacctg tcttcatttg aataagatgt tagcaattgt ttttagcttt gtcttgttgt 29220 ggcagggcgg caagtgcttc agacatcatt ctgttttcaa attttatgct ggagaacagc 29280 ttcttaattc ctttggaaat aatagactgc gtcttaaaat tcagatgtct ggatatagat 29340 atgattgtaa aataacctat ttaagtgtca tttagaacat aagttttatg aatgttcttc 29400 cattttcgtc atcgaacgaa taagagtaaa tacacctttt ttaacattac aaataagttc 29460 ttatacgttg tttatacacc gggaatcatt tccattattt tcgcgcaaaa gtcacggata 29520 ttcgtgaaag cgacataaac tgcgaaattt gcggggagtg tcttgagttt gcctcgaggc 29580 tagcgcatgc acatagacac acacatcatc tcattgatgc ttggtaataa ttgtcattag 29640 attgttttta tgcatagatg cactcgaaat cagccaattt tagacaagta tcaaacggat 29700 gtgacttcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt 29760 gatttacaat tgaatatatc ctgccccagc cagccaacag ctcgatttac aattgaatat 29820 atcctgccgg ccggcccacg cgtgtcgagg aattctgatc tggcccccat ttggacgtga 29880 atgtagacac gtcgaaataa agatttccga attagaataa tttgtttatt gctttcgcct 29940 ataaatacga cggatcgtaa tttgtcgttt tatcaaaatg tactttcatt ttataataac 30000 gctgcggaca tctacatttt tgaattgaaa aaaaattggt aattactctt tctttttctc 30060 catattgacc atcatactca ttgctgatcc atgtagattt cccggacatg aagccattta 30120 caattgaata tatcctgccg ccgctgccgc tttgcacccg gtggagcttg catgttggtt 30180 tctacgcaga actgagccgg ttaggcagat aatttccatt gagaactgag ccatgtgcac 30240 cttcccccca acacggtgag cgacggggca acggagtgat ccacatggga cttttaaaca 30300 tcatccgtcg gatggcgttg cgagagaagc agtcgatccg tgagatcagc cgacgcaccg 30360 ggcaggcgcg caacacgatc gcaaagtatt tgaacgcagg tacaatcgag ccgacgttca 30420 cgcggaacga ccaagcaagc ttggctgcca tttttggggt gaggccgttc gcggccgagg 30480 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg 30540 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca 30600 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 30660 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 30720 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat 30780 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 30840 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 30900 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 30960 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 31020 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct 31080 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg 31140 agcgtcggaa agggtcgacg gatcttttcc gctgcataac cctgcttcgg ggtcattata 31200 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt 31260 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc 31320 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac 31380 gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg 31440 atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt 31500 ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc 31560 tacctgctgg ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc 31620 cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct gaaactctgg 31680 ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg 31740 aagatcgaag agaagcagga cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg 31800 gcagagccat gactttttta gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca 31860 cgtccccatg cgctccatca agaagagcga cttcgcggag ctggtattcg tgcagggcaa 31920 gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg acttcattgc 31980 cgataaggtg gattatctgg acaccaaggc accaggcggg tcaaatcagg aataagggca 32040 cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga 32100 ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg atgccgaaac 32160 catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat 32220 ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc cccctgccct 32280 gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc gaacaggagg cggcaggttt 32340 ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc gaaaaaccgc 32400 cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc tgaaacacac 32460 gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt ggccggacac 32520 gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc gcaacaagaa 32580 aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg acgtgaagat 32640 cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt 32700 ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct acgagctttg 32760 ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg aatgcctgtc 32820 gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc tggaatcggt 32880 gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt 32940 cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga aattcatatg 33000 ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt tcagctcgca 33060 ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat cggattccac 33120 ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg 33180 cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct agggccttgt 33240 ggggtcagtt ccggctgggg gttcagcagc cagcgcttta ctggcatttc aggaacaagc 33300 gggcactgct cgacgcactt gcttcgctca gtatcgctcg ggacgcacgg cgcgctctac 33360 gaactgccga taaacagagg attaaaattg acaattgtga ttaaggctca gattcgacgg 33420 cttggagcgg ccgacgtgca ggatttccgc gagatccgat tgtcggccct gaagaaagct 33480 ccagagatgt tcgggtccgt ttacgagcac gaggagaaaa agcccatgga ggcgttcgct 33540 gaacggttgc gagatgccgt ggcattcggc gcctacatcg acggcgagat cattgggctg 33600 tcggtcttca aacaggagga cggccccaag gacgctcaca aggcgcatct gtccggcgtt 33660 ttcgtggagc ccgaacagcg aggccgaggg gtcgccggta tgctgctgcg ggcgttgccg 33720 gcgggtttat tgctcgtgat gatcgtccga cagattccaa cgggaatctg gtggatgcgc 33780 atcttcatcc tcggcgcact taatatttcg ctattctgga gcttgttgtt tatttcggtc 33840 taccgcctgc cgggcggggt cgcggcgacg gtaggcgctg tgcagccgct gatggtcgtg 33900 ttcatctctg ccgctctgct aggtagcccg atacgattga tggcggtcct gggggctatt 33960 tgcggaactg cgggcgtggc gctgttggtg ttgacaccaa acgcagcgct agatcctgtc 34020 ggcgtcgcag cgggcctggc gggggcggtt tccatggcgt tcggaaccgt gctgacccgc 34080 aagtggcaac ctcccgtgcc tctgctcacc tttaccgcct ggcaactggc ggccggagga 34140 cttctgctcg ttccagtagc tttagtgttt gatccgccaa tcccgatgcc tacaggaacc 34200 aatgttctcg gcctggcgtg gctcggcctg atcggagcgg gtttaaccta cttcctttgg 34260 ttccggggga tctcgcgact cgaacctaca gttgtttcct tactgggctt tctcagcccc 34320 cgagcgctta gtgggaattt gtacccctta tcgaaccggg agcacaggat gacgcctaac 34380 aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga 34440 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc 34500 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc 34560 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa 34620 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga 34680 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc 34740 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct 34800 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata 34860 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc 34920 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg 34980 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa 35040 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc 35100 ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg gcctcgcgcg 35160 cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca 35220 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg 35280 ttagagagct ggggaagact atgcgcgatc tgttgaaggt ggttctaagc ctcgtcttgc 35340 gatggcattt cgatccattc ccattccgcg ctcaagatgg cttcccctcg gcagttcatc 35400 agggctaaat caatctagcc gacttgtccg gtgaaatggg ctgcactcca acagaaacaa 35460 tcaaacaaac atacacagcg acttattcac acgagctca 35499 <210> 69 <211> 22042 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB108224 <400> 69 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agtgatcagc agagtgagtc ctgagtcctg 240 agtatcactc atcttaatta agcggccgca tcagtaagtg catataaaga aagtaataag 300 tagatgagtt tggtaattac atgagatcga cagaggaatt acgacgttct tgccatcaaa 360 aagagattcc aaatttgtaa agagccatat gtttgttggg caattgagat tgagtcttgc 420 caatccttct ttggaagttg acaatttaaa aattcgaagc cagatttact cctaattaat 480 tattgagctt gaagtcttaa ttaccgatta catctctgag cagtagtagt cgatgatctt 540 tcccggtcac taattttact caagcgaatc gatttttact ttagtttagc taaccgtacg 600 ccattacagt ttagagagca gcaagggtaa gaacattatt aaaacataaa gtaaacctct 660 catttattac ggtgtagtgc taattacatt agcctcaaac atcctaagct tacagtttta 720 taaaacacac acgatgactg tgtatagtat taacatacat caatagcaac actcatgatc 780 atcgtgctag gccgggcttg gactgggtga cctaagtgat taagctaact actcagaacg 840 caagtgagtc tgtcgcaatg acagaggctt gaacatcaat gaagagatca ccttgttcgt 900 tgtggaactg aagtgcatgt ttgtgctggc tatgtccacc actctgattg gatctgaggg 960 tgatgtagaa gggtttgtct tgtgggaccg gtctgtgttg gactatcctc tgaatggagt 1020 tcgggagtgc agcttgtccc agcgttctcc tcacccagac caacattgct tgaaaggcaa 1080 gatcattgac aaagggatca tgtgcatcag tgtcagttgc aaactctcct ctggctgcat 1140 ctgatccagg aacagcagaa cacttggcaa caagctggct cttggtgcag ctgagaacgt 1200 catcaatgcc tctgaacgca ggaccatgaa agagagtctt gccatcatag actgaccctt 1260 gcaaagcagg atctgcatca aggctcggag gctgcatggt tgcattggca ggtggagcac 1320 cttggttgga caacactatg acagctctgt aggctggaac aagttttcca cttgagaacg 1380 tcttgagcgt cgcttggaca ttcaccctcc cactcggagc agtggacggt gtgagggtca 1440 cttcacagtt gacgtctcca tcaacagtga cacctttgaa gagctgtgcg tcatctatgg 1500 cccagagaga gtagccaggg aagagtccaa ggcaggtctc agcaaggctc ccaatagcaa 1560 gggtcattgg caacaccctt ctgccttgta tgacatgatc ctccaagaag ggattggact 1620 tggcactgat ctttctatgc agggtgatcg tgtcacttcc aactttcttg gatggtgtcc 1680 tccagtttcc aacaaggatc tcagcaggac ttgatcccaa cacaatcctc gcaactgtat 1740 cagcacctcc ttcacgagga atgatctgaa ctcccatttc ttgaaactgt ttcttgagtt 1800 gaggtgtcac catcccacca tcccagggac caaagcagat ggacttgaca ctgacatcct 1860 tcgcaagttc caaacccatc ttgttgagag cttcgtttgc catcgcatag tcagactgac 1920 caacattgcc gtggaaacca gcaagggagc tgaagagaac catgtgtttg aggttggcac 1980 gatcaactgc agccagaaga ttctccaacc cagtgacttt ggtcccaaag acagcatcaa 2040 actcgtcagg gagtttcttc tctatgagac gatcacggag cacaccagaa gcgtgaacga 2100 ttccactaac tctggcacca agctgggact ctgcatccct aactgctttg gcaacatctg 2160 cagcagagtt cacatcacaa gagctgtaga tcgctttgcc acccagagct tcaatggcag 2220 caatggagct tctcacttca cgagcaccaa gaacagatcc cacaagtttc gtgacagccc 2280 ttggagtggg tttgggtcct tcaccagcac tgaaggccct cttcaactct tgggtggcag 2340 ctttctgcac agccttctca tcagtgatgc cagcacacca tgctggttca gacgcagaaa 2400 ctttgctacg acccaacaga atgtacttgc ctccagcaat ctgacgcgtg atctcacgga 2460 tgcaaagagg tgtgatccca cgagcacctc cagaaaccag aagcacatcg tctttcgcta 2520 tctgtctctg agggttccca gtctccaact tggcagctct gattgtgcat ctttgctggt 2580 ttgctccaat cccaacctct ctgattctga tgtcagcaca cgccatctcc ctaactatgg 2640 caactgcagc atcttctggg tgcatccctt gagcaatgtc cacaccacgg ctgaacacat 2700 cagattctga ccactccaac cctattgtct tgcagagtcc aaagatcgct ccacgctgtg 2760 ctctcttgag tgcgtcagaa gtgccttgag aggtgaatcc aagtctgccg tcaagcctag 2820 ccacaccgat gaaggcagga cgacctccag ccacagcagt gcagagagag gctttggcaa 2880 acttcgcaca catgagtgtg aaacccagta tctcagctgg ttcaaacctt tctgcttgct 2940 ggcttatgaa gcctcccaac gctccaaatc tttgctcagc ctctttgatg gccttctcag 3000 ctgattctgc tgaaaggtcc ttagccagaa catgacgaat ggcagctgca ccagctctct 3060 gagccaactg caatccctca aaggtcaaga caactgcaca ggctcccaac accctcacaa 3120 gagcaagtgt caactctgag ccatcgtcaa cgacaagaac tggacggttc tccggtcttt 3180 ccagaatcag ttcatctggc cttctgatgt caaccacctt tgcgtgcatc agtgacaaat 3240 cgtcacagtc agcaccatga actgaatcaa tcttcacagc tggtgcagca ccggcacttg 3300 caggagcggc tgcagccgga gcaggggctg atccaccagc tatctcagcc ttcatggcat 3360 cgaccacttc tccaaccgtt ctggtccttg acaaagcatc gacatctttg gcctcaacgt 3420 tcagcatggc ttgaacctca gaaaggattt ccacacgctt gatgctatca atcccaagct 3480 ctgtttccaa ctccatgtct gactctatca tgtcagtctc gtatccagtc tttgcagcca 3540 aaacctccat cacaacagtt tcagcttttt caaggagttc gttgctgaca gctggtgccg 3600 cggcagcggg agcaggagcg gctgcagctg gcgcaggagc agaaccccca gctatctcag 3660 ccttcatggc atcaaccacc tctcccaccg tccttgttct ggaaagagca tcaacatctt 3720 tggcctccac attcaacatc gcttgaacct cagagaggat ttcaacacgt ttgatggagt 3780 caattccaag ctcggtctcc agttccatgt cagattcaat catgtcggtc tcatagccag 3840 ttttcgcagc aaggacttcc atcacaactg tctcagcctt ttccaggagt tctgaggaga 3900 cggccggtgc ggctgcagct ggagccggtg ccgcggctgc gggtgcagga gcggctgcag 3960 ctggcgcagg cgcggaactt ccagcgatct ccgccttcat cgcatcaacg acctcaccaa 4020 ctgttctggt ccttgagagg gcatccacat ccttagcctc cacattgagc attgcttgga 4080 cttcactgag gatctcaaca cgcttgatgc tgtcaatgcc caattccgtc tcaagttcca 4140 tgtcagactc aatcatgtca gtctcatagc cggtttttgc agccagcact tccatgacaa 4200 ctgtttcagc cttctcaagc aattcgttgc tgacagctgg tgccgcggct gcgggtgcag 4260 gtgcggcagc tgccggcgca ggagcagagc ctccagcaat ctcagccttc atcgcatcga 4320 caacttcccc cacagttctt gtcctactga gtgcatcaac atctttagcc tcaacgttga 4380 gcatggcttg cacctcagag aggatctcca ctctcttgat ggaatctatt ccaagttcgg 4440 tctccaattc catgtctgac tcaatcatgt ccgtttcgta accagttttt gcagcaagga 4500 cttccatcac aactgtttcg gctttctcca gcaactcatt gctgacggca ggagcggctg 4560 cagctggagc aggtgccgcg gcagcaggtg caggtgcggc tgcagctggc gcactagcag 4620 agcttccggc aatttcagcc ttcatggcgt ccacgacctc accgactgtt ctggttcttg 4680 aaagcgcatc gacatctttt gcctccacgt tcaacatagc ttggacctca gacaagattt 4740 caacacgttt gatgctatct attcccaact cggtttcaag ctccatgtca ctttcaatca 4800 tgtcggtctc gtaaccagtc ttagctgcga gcacctccat cacaaccgtc tcggcctttt 4860 ccaggagttc ggatgaaaca gctggcgcgg gcgcggccgg tgctggtgca gctggagctg 4920 gagctgcggc tgccggtgca ggtgccgcgg cagctggcgc aggcgcgctc cctccagcaa 4980 tctccgcctt catggcatcc acaacctcac ccactgtcct tgttcttgaa agtgcatcaa 5040 catccttagc ttcaacattg agcatggctt ggacttcact caggatctcc actcttttga 5100 tggaatcgat cccaagctct gtctccaatt ccatatcaga ttcaatcatg tcggtctcat 5160 agccagtctt tgcagccaaa acctccatca caaccgtctc agccttctcc aagagttctg 5220 aagaaacggc aggtgcaggt gcagctggag caggtgccgc ggctgcgggt gcaggagcgg 5280 ctgcagctgg cgcgggcgca gaccctccag caatttcagc cttcatggca tccacaacct 5340 caccaacggt cctcgttctg gacagagcat caacatcctt cgcttccacg ttcagcatag 5400 cttggacctc actgaggatt tcaacacgtt tgatgctgtc tatcccaagt tctgtctcca 5460 attccatgtc agactcaatc atgtcagtct catagccggt tttagctgcc aagacctcca 5520 tcacgacggt ctcagctttt tcaaggagtt cgtttgagac ggccggagcg gctgcagctg 5580 gagctggagc cgcggcagcg ggaccaggag cggcagcggc cggcgcagga gcggagcctc 5640 cagctatctc agccttcatg gcattgacaa cctcaccgac agttctggtc cttgagagag 5700 catccacatc cttagcttcc acattgagca tggcttggac ttcactgagg atttccacac 5760 gcttgatgga atcaattcca agctccgtct caagctccat gtctgattct atcatgtcag 5820 tttcgtagcc agttttggca gcaaggactt ccatcacaac agtttctgcc ttttcgagca 5880 gctcattaga aacagctggt gcagctgcag ctggagccgc tttagccggt gcaggtgcgg 5940 cagctgccgg cgccggagcg gaagaaccag cgatttctgc cttcatagcg ttgacaactt 6000 cacccactgt tctcgtccta gagagagcat caacatcttt cgcctcaacg ttaagcatag 6060 cttggacttc agaaagaatt tcaaccctct tgatgctgtc aattcccaac tcagtctcaa 6120 gctccatgtc cgcctcaatc atgtctgttt cgtatccagt cttggcagcg agaacctcca 6180 tcacaactgt ctcagctttc tcaagcaact cgttggacac ggcgggagca ggagcacttg 6240 caacgggagc ggcaggggca gcggctttaa caggcgccgg agcagtctgt tgctgttgct 6300 gttgctgaag ggaagaagca tcaacacttc cataaccatc cagctcagcc agcatggact 6360 tgaggatggc acgatgtttc tccacaacag cagtgtcaac tgcaggcttg gctgaagctg 6420 cagcagtctt ggcttcctcc ttggcagcag caagtttgga gttggcttct gcagctgcac 6480 gtttggcgtc gtcaagctgc ctctgagcgt cttgaagctc cttctgaagc ctttcagcct 6540 ccctcttggc agcttcgtca accacaggtt caggagcctt gatgagtggt gcagcaccct 6600 tgaggtatgt gacacatctt ccgtcgttca tggcagcatc cctcactttc ttggtcttgt 6660 cactgacata ggtggcagct gaaagtctga gtgtggttct ctttttcttg atggcttgca 6720 tcctcgtggc atcaggagca tcccacttgt caaacccttg aagattgaca ccagccacaa 6780 ccagctgaac tgcagcatcc ctcaactgta tgtcagagtc cgtgccagaa gctgggttca 6840 cagacactgt gacaacagac gggtcatctt tgagggtttc agaaacaagt ttgctgagaa 6900 cttgtttcgg tccaaactca acaaagattc tcgcaccagc ttggtgcatg ttcctcactt 6960 gggtcaggaa cttcacagat gaggtcatgt gttgagtgag catctctctg gcatccgtcg 7020 gataggtctc tccagaaaca ttgctgaaga gctttgtttc tgctttgggt gtacggaagg 7080 aaaccttgga tatgacatct ttgaaagctg agctggcatt ttccatctgt ggagaatgga 7140 aggcagactc acaagcaaga ggcacaactc tgaacccttc cttctgcaat ctggcacttt 7200 cagcttgaat cccttcaaca gacccagtaa tgacagtctg agacggagag tttgagtttc 7260 caagccagac gttggcagct tgaaccttga tgttctctgc gttgggtcct atgacagctg 7320 ccatgcaacc tttgggtgtg gcaggtgcat ctttgcctcc catgatcctt gcccttctgc 7380 aaacaagttc aaacaactca tctctgtcaa cacaaccagc tgcatagagc gcagcaaact 7440 cacccaagct gtgtccagct gcaaagtctg gagtgaatcc agcttccttg aaaatctcaa 7500 aggcaccaag agcacatgcc agggttgacg gctggctgta tgctgtgagt gagattttct 7560 tgtgatcttg ttctggctca cgttcgtacg gtttccttgg gtacaggact tgagagactc 7620 tttcaaagtc tttgtcactt ccagcaacct tggactgtgc agcgtccatt gcagcaatgg 7680 actgtctgaa ctgaggccag ttcatcgcaa cttcactgaa catgtgtgtg tactgagcac 7740 cttgtccaga gaagagtgca gcaacagcac cattggtggc aatgcctttg gcacggaaag 7800 aaactccttc tctcggaagc ctccacgctt ctttggtcac atctttggca aactgagcac 7860 atatagcacg aagcgtggag cacgcatcct ccgcatcttt gaccaagaat cccagtctcg 7920 cattggtcgc aggtatgctc ccagggaact tgaactgttc tccaaacttg acacacttga 7980 tgtaggcagt attcttgact gtctcattct ccttgatcgc agcttcaaac tctttcagct 8040 gagcttcaca aaggctctgc aacgcagcag gagttgcagc catcatcaac accggttgtg 8100 gacgtttgtt gagtctgtac gctgtcgtgt gctctggttc agcctcttcc aacactgcat 8160 ggtagttggc acctccaaag ccaaagctgg atattccagc acgtctcgga acaccaggtg 8220 gagggaacca gggtctgttc atcgtgttga tgtagaggga tgactcattg atgggagtgt 8280 tgtcatagag attgggtgga ttgtccacat tgatcgttcc aggcaaagtc ttgtgtttga 8340 gagccatgat gaccttgatc attccagcaa gaccagcaac agccttcaaa tgaccaatac 8400 tggacttgat acttccaact gcaactttct ctgtgttccc ttcaccatac gctttgtcaa 8460 acaagttcct caacgcagtc aactcaatcc tatctccaac tggagtgcca gtcccatgac 8520 cctcaaccaa agtgacagtg gcagggtcaa cgcaggcacg attgtatgca cgtctcaagg 8580 cttcctcttg accactgatt gtgggagtgt agatgccagc tgctttgcca tcacttgagc 8640 ttgcacaacc acgaatcaca gcatgaatct catcaccatc cctcactgca tctgcataac 8700 gtttgagcac caacatggct gatccctcac caatgagcat ccctttcgtt ttctcatcat 8760 acgctctcac acttgggtcc gtgctgaaca caggtgtctt tgagaaagcc atgtacatcc 8820 caatggagtt gtctgtgcag gttgcaccag tgaccatcat gtcgcagtca ccatagagca 8880 gttcatcaat cgcaaccttg actgctatca aggagcttgc acatgcagca tccacaacgc 8940 agttcatgcc atccaagttg aatgtgttgg tgcatctgcc agcagtgaca ttcccaagga 9000 atccagggaa agagtcaagt ctccattctg ggaagttcgc tttgtacttc tccacagcca 9060 ctttgacatc ttcctctggc atccccatct ttctcaagac tttctccaca accacatagt 9120 tgagtctgct gtagaactca tgtgaactct tctgtccacc tccaatgcca agcacgcatc 9180 ctatgttttt cttctccttt ccaagtgcat caatcccagc atcttgaagt gcttctttga 9240 ccttgagaag tgagatggtc tgattcgcat cagaatcttc catctggaac atgttgagac 9300 caaactcacg cgcatcaaag tcatactcag gaatgaaacc tcctctcttg cagtagattt 9360 tgtcctttgt ggtcttgact gggtcaaagt acgcagtcac atcaacacga tcctcaggaa 9420 gatcagacag gcaatcaatg ccagccctta tggtctccca tgactctctc accgtggttc 9480 cacaaggaag tatggcgctc atgcctatga tcgcaatcct cgtgtccatc tcacctcctt 9540 tctgctcttg cagcctcgct gccatttttg gttgtggatc cgttttgcta tttgtgtatg 9600 ttttcttgtt ttggtttgag tttaaccatc attctcttct ctctatttat agagagaaga 9660 ggttggcacg cagcactgcg acatgagaga aagacacggg tgaagaggcg ggagctgagg 9720 tggcagcatg catgcatgca tggccagtcg tgtcatagtg agaagaggag aaagctgagg 9780 tggcaacgtg tatggccagt ggtgtcatag tgtgaaagct gacgtggcag catgcatggt 9840 gagtgtgaaa aaggcgccac agtgcagatg ttactgaaca aaagcggtgt gccatgcaac 9900 ttttgcccat cgtcgtcttt ctcatccaag ttcctcttta taatatcttt taccaaatca 9960 tattttaata taaattcatt ttatttttaa ataaagtaat attatatcca taaatattaa 10020 ctctcattta acacattttc aaatttaaca cttctgtaaa taaatttatt aacaaaaact 10080 tgtaaatatg ttcaaataaa tccataaata ttaactttca ttttattaac aaaaaaatct 10140 ttactataag ctgcggccgc ttaattaaga tgagtgatac tcaggactca ggactcactc 10200 tgctgatcac tagtgctagc ctcgaggtcg acacaacttt gtatacaaaa gttgtctagg 10260 atctgctttt acatgcgaga cacatcttct aaagtaattt taataatagt tactatattc 10320 aagatttcat atatcaaata ctcaatatta cttctaaaaa attaattaga tataattaaa 10380 atattacttt tttaatttta agtttaattg ttgaatttgt gactattgat ttattattct 10440 actatgttta aattgtttta tagatagttt aaagtaaata taagtaatgt agtagagtgt 10500 tagagtgtta ccctaaacca taaactataa gatttatggt ggactaattt tcatatattt 10560 cttattgctt ttaccttttc ttggtatgta agtccgtaac tggaattact gtgggttgcc 10620 atgacactct gtggtctttt ggttcatgca tggatcttgc gcaagaaaaa gacaaagaac 10680 aaagaaaaaa gacaaaacag agagacaaaa cgcaatcaca caaccaactc aaattagtca 10740 ctggctgatc aagatcgccg cgtccatgta tgtctaaatg ccatgcaaag caacacgtgc 10800 ttaacatgca ctttaaatgg ctcacccatc tcaacccaca cacaaacaca ttgccttttt 10860 cttcatcatc accacaacca cctgtatata ttcattctct tccgccacct caatttcttc 10920 acttcaacac acgtcaacct gcatatgcgt gtcatcccat gcccaaatct ccatgcatgt 10980 tccaaccacc ttctctctta tataatacct ataaatacct ctaatatcac tcacttcttt 11040 catcatccat ccatccagag tactactact ctactactat aataccccaa cccaactcat 11100 attcaatact actctaggat ccacaaccaa aaatgcttca gcacacttgg cttccgaagc 11160 ctcccaatct gaccctcttg tcagatgagg ttcatctctg gaggattcct cttgaccagc 11220 ctgagtcaca acttcaagac cttgctgcca ccctgagcag tgatgaattg gcgagggcaa 11280 acagattcta ctttccagaa cacagaaggc gtttcactgc tgggagaggc atcctcagat 11340 ccatcttggg tggatacttg ggagtggaac cgggtcaagt caagtttgat tatgagtccc 11400 gtgggaaacc gatccttggt gacagatttg ctgagagtgg actcctgttc aacttgtctc 11460 acagccagaa ccttgccttg tgtgctgtca actacacgcg tcaaataggc attgatcttg 11520 aatatctgcg tccaacatct gacttggagt ctcttgcaaa gaggttcttt ctcccaagag 11580 aatatgaact cttgaggtca ctccctgatg agcagaaaca gaagattttc tttcgttact 11640 ggacttgcaa agaggcttat ctcaaagcaa cgggagatgg aatagccaaa cttgaagaga 11700 tcgagatagc actcacccca acagaacctg ccaagctcca aacagctcct gcgtggtctc 11760 tgttggagct tgtgccagat gacaattgtg ttgcagctgt ggctgttgcg ggttttggtt 11820 ggcagcccaa gttctggcat tactgagtag ttagcttaat cacttaggtc acctcgagta 11880 tcaaaatcta tttagaaata cacaatattt tgttgcaggc ttgctggaga atcgatctgc 11940 tatcataaaa attacaaaaa aattttattt gcctcaatta ttttaggatt ggtattaagg 12000 acgcttaaat tatttgtcgg gtcactacgc atcattgtga ttgagaagat cagcgatacg 12060 aaatattcgt agtactatcg ataatttatt tgaaaattca taagaaaagc aaacgttaca 12120 tgaattgatg aaacaataca aagacagata aagccacgca catttaggat attggccgag 12180 attactgaat attgagtaag atcacggaat ttctgacagg agcatgtctt caattcagcc 12240 caaatggcag ttgaaatact caaaccgccc catatgcagg agcggatcat tcattgtttg 12300 tttggttgcc tttgccaaca tgggagtcca aggttggcgc gccgaattcg cggccgctta 12360 attaactact gtcactgagg ccgtagacga gtacggactg atctaactag tgctagcctc 12420 gaggtcgaca cccagctttc ttgtacaaag tggttgcggc cgcttaatta aatttaaatt 12480 caattaatgc aatcttgatt ttcaacaacg aaggtaatgg cgtaaaagaa aaaatgtatg 12540 ttattgtatt gatctttcat gatgttgaag cgtgccataa tatgatgatg tataattaaa 12600 atattaactg tcgcatttta ttgaaatggc actgttattt caaccatatc tttgattctg 12660 ttacatgaca cgactgcaag aagtaaataa tagacgccgt tgttaaagaa ttgctatcat 12720 atgtgcctaa ctagagggaa tttgagcgtc agacctaatc aaatattaca aaatatctca 12780 ctctgtcgcc agcaatggtg taatcagcgc agacaaatgg cgtaaagatc gcggaaaaac 12840 ctccccgagt ggcatgatag ctgcctctgt attgctgatt tagtcagcct tatttgactt 12900 aagggtgccc tcgttagtga caaattgctt tcaaggagac agccatgccc cacactttgt 12960 tgaaaaacaa attgcctttg gggagacggt aaagccagtt gctcttcaat aaggaatgtc 13020 gaggaggcaa tgtaaccgcc tctggtagta cacttctcta atccaaaaat caatttgtat 13080 tcaagatacc gcaaaaaact tatggtttaa accctgcagg actagtccag aaggtaatta 13140 tccaagatgt agcatcaaga atccaatgtt tacgggaaaa actatggaag tattatgtaa 13200 gctcagcaag aagcagatca atatgcggca catatgcaac ctatgttcaa aaatgaagaa 13260 tgtacagata caagatccta tactgccaga atacgaagaa gaatacgtag aaattgaaaa 13320 agaagaacca ggcgaagaaa agaatcttga agacgtaagc actgacgaca acaatgaaaa 13380 gaagaagata aggtcggtga ttgtgaaaga gacatagagg acacatgtaa ggtggaaaat 13440 gtaagggcgg aaagtaacct tatcacaaag gaatcttatc ccccactact tatcctttta 13500 tatttttccg tgtcattttt gcccttgagt tttcctatat aaggaaccaa gttcggcatt 13560 tgtgaaaaca agaaaaaatt tggtgtaagc tattttcttt gaagtactga ggatacaact 13620 tcagagaaat ttgtaagttt gtaggtacca gatctggatc ccaaaccatg tctccggaga 13680 ggagaccagt tgagattagg ccagctacag cagctgatat ggccgcggtt tgtgatatcg 13740 ttaaccatta cattgagacg tctacagtga actttaggac agagccacaa acaccacaag 13800 agtggattga tgatctagag aggttgcaag atagataccc ttggttggtt gctgaggttg 13860 agggtgttgt ggctggtatt gcttacgctg ggccctggaa ggctaggaac gcttacgatt 13920 ggacagttga gagtactgtt tacgtgtcac ataggcatca aaggttgggc ctaggatcta 13980 cattgtacac acatttgctt aagtctatgg aggcgcaagg ttttaagtct gtggttgctg 14040 ttataggcct tccaaacgat ccatctgtta ggttgcatga ggctttggga tacacagccc 14100 ggggtacatt gcgcgcagct ggatacaagc atggtggatg gcatgatgtt ggtttttggc 14160 aaagggattt tgagttgcca gctcctccaa ggccagttag gccagttacc caaatctgag 14220 tagttagctt aatcacctag agctcgatcg gcggcaatag cttcttagcg ccatcccggg 14280 ttgatcctat ctgtgttgaa atagttgcgg tgggcaaggc tctctttcag aaagacaggc 14340 ggccaaagga acccaaggtg aggtgggcta tggctctcag ttccttgtgg aagcgcttgg 14400 tctaaggtgc agaggtgtta gcgggatgaa gcaaaagtgt ccgattgtaa caagatatgt 14460 tgatcctacg taaggatatt aaagtatgta ttcatcacta atataatcag tgtattccaa 14520 tatgtactac gatttccaat gtctttattg tcgccgtatg taatcggcgt cacaaaataa 14580 tccccggtga ctttctttta atccaggatg aaataatatg ttattataat ttttgcgatt 14640 tggtccgtta taggaattga agtgtgcttg aggtcggtcg ccaccactcc catttcataa 14700 ttttacatgt atttgaaaaa taaaaattta tggtattcaa tttaaacacg tatacttgta 14760 aagaatgata tcttgaaaga aatatagttt aaatatttat tgataaaata acaagtcagg 14820 tattatagtc caagcaaaaa cataaattta ttgatgcaag tttaaattca gaaatatttc 14880 aataactgat tatatcagct ggtacattgc cgtagatgaa agactgagtg cgatattatg 14940 gtgtaataca taggaattcg tttaaacgat ctgcgtctaa ttttcggtcc aacttgcaca 15000 ggaaagacgt cgaccgcggt agctcttgcc cagcagactg ggcttccagt cctttcgctc 15060 gatcgggtcc aatgttgtcc tcagctgtga accggaagcg gacgaccaac agtggaagaa 15120 ctgaaaggaa cgagccgtct ataccttgat gatcggcctc tggtgaaggg tatcatcgca 15180 gccaagcaag ctcatgaaag gctgatgggg gaggtgtata attatgaggc ccacggcggg 15240 cttattcttt agggaggatc tatctcgttg ctcaagtgca tggcgcaaag cagttattgg 15300 agtgcggatt ttcgttggca tattattcgc cacgagttag cagacgaaga gaccttcatg 15360 aacgtggcca aggccagagt taagcagatg ttacgccctg ctgcaggcct ttctattatc 15420 caatagttgg ttgatctttg gaaagagcct cggctgaggc ccatactgaa agagatcgat 15480 ggatatcgat atgccatgtt gtttgctagc cagaaccaga tcacatccga tatgctattg 15540 cagcttgacg cagatatgga ggataagttg attcatggga tcgctcagga gtagctcatc 15600 catgcacgcc gacaagaaca gaaattccgt cgagttaacg cagccgctta cgacggattc 15660 gaaggtcatc cattcggaat gtattagttt gcaccagctc cgcgtcacac ctgtcttcat 15720 ttgaataaga tgttagcaat tgtttttagc tttgtcttgt tgtggcaggg cggcaagtgc 15780 ttcagacatc attctgtttt caaattttat gctggagaac agcttcttaa ttcctttgga 15840 aataatagac tgcgtcttaa aattcagatg tctggatata gatatgattg taaaataacc 15900 tatttaagtg tcatttagaa cataagtttt atgaatgttc ttccattttc gtcatcgaac 15960 gaataagagt aaatacacct tttttaacat tacaaataag ttcttatacg ttgtttatac 16020 accgggaatc atttccatta ttttcgcgca aaagtcacgg atattcgtga aagcgacata 16080 aactgcgaaa tttgcgggga gtgtcttgag tttgcctcga ggctagcgca tgcacataga 16140 cacacacatc atctcattga tgcttggtaa taattgtcat tagattgttt ttatgcatag 16200 atgcactcga aatcagccaa ttttagacaa gtatcaaacg gatgtgactt cagtacatta 16260 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgatttac aattgaatat 16320 atcctgcccc agccagccaa cagctcgatt tacaattgaa tatatcctgc cggccggccc 16380 acgcgtgtcg aggaattctg atctggcccc catttggacg tgaatgtaga cacgtcgaaa 16440 taaagatttc cgaattagaa taatttgttt attgctttcg cctataaata cgacggatcg 16500 taatttgtcg ttttatcaaa atgtactttc attttataat aacgctgcgg acatctacat 16560 ttttgaattg aaaaaaaatt ggtaattact ctttcttttt ctccatattg accatcatac 16620 tcattgctga tccatgtaga tttcccggac atgaagccat ttacaattga atatatcctg 16680 ccgccgctgc cgctttgcac ccggtggagc ttgcatgttg gtttctacgc agaactgagc 16740 cggttaggca gataatttcc attgagaact gagccatgtg caccttcccc ccaacacggt 16800 gagcgacggg gcaacggagt gatccacatg ggacttttaa acatcatccg tcggatggcg 16860 ttgcgagaga agcagtcgat ccgtgagatc agccgacgca ccgggcaggc gcgcaacacg 16920 atcgcaaagt atttgaacgc aggtacaatc gagccgacgt tcacgcggaa cgaccaagca 16980 agcttggctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg 17040 gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg 17100 cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg 17160 tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca 17220 aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct 17280 gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc 17340 cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa 17400 actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg 17460 ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt 17520 gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg 17580 ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc 17640 atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg 17700 acggatcttt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata 17760 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg 17820 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg 17880 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg 17940 aggctggccg gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa 18000 gccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc 18060 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg 18120 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat 18180 caatggcgac ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg 18240 cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca 18300 ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt 18360 ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca 18420 tcaagaagag cgacttcgcg gagctggtat tcgtgcaggg caagattcgg aataccaagt 18480 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc 18540 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag 18600 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc 18660 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg 18720 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg 18780 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg 18840 ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag tcgatgacca 18900 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa 18960 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg 19020 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa 19080 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc 19140 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg 19200 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca 19260 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt 19320 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg 19380 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct 19440 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa 19500 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc 19560 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc 19620 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc 19680 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct 19740 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg 19800 ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact gctcgacgca 19860 cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc cgataaacag 19920 aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag cggccgacgt 19980 gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga tgttcgggtc 20040 cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc 20100 cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct tcaaacagga 20160 ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg agcccgaaca 20220 gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt 20280 gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca tcctcggcgc 20340 acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg 20400 ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct 20460 gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa ctgcgggcgt 20520 ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg cagcgggcct 20580 ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt 20640 gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc tcgttccagt 20700 agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc tcggcctggc 20760 gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg ggatctcgcg 20820 actcgaacct acagttgttt ccttactggg ctttctcagc ccccgagcgc ttagtgggaa 20880 tttgtacccc ttatcgaacc gggagcacag gatgacgcct aacaattcat tcaagccgac 20940 accgcttcgc ggcgcggctt aattcaggag ttaaacatca tgagggaagc ggtgatcgcc 21000 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg 21060 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat 21120 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc 21180 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa 21240 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg 21300 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc 21360 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt 21420 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat 21480 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg 21540 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc 21600 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct 21660 aggcaggctt atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa 21720 tttgttcact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt 21780 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagaga gctggggaag 21840 actatgcgcg atctgttgaa ggtggttcta agcctcgtct tgcgatggca tttcgatcca 21900 ttcccattcc gcgctcaaga tggcttcccc tcggcagttc atcagggcta aatcaatcta 21960 gccgacttgt ccggtgaaat gggctgcact ccaacagaaa caatcaaaca aacatacaca 22020 gcgacttatt cacacgagct ca 22042 <210> 70 <211> 23255 <212> DNA <213> Artificial sequence <220> <223> plasmid pDAB108225 <400> 70 aattacaacg gtatatatcc tgccagtcag catcatcaca ccaaaagtta ggcccgaata 60 gtttgaaatt agaaagctcg caattgaggt ctacaggcca aattcgctct tagccgtaca 120 atattactca ccggatccta accggtttaa ttaaggcgcg ccatgcccgg gcaagcggcc 180 gcacaagttt gtacaaaaaa gcaggcttct agacctagga ctaggcactg agtgactcac 240 tgacctgact gagtgactgg cggccgcatc agtaagtgca tataaagaaa gtaataagta 300 gatgagtttg gtaattacat gagatcgaca gaggaattac gacgttcttg ccatcaaaaa 360 gagattccaa atttgtaaag agccatatgt ttgttgggca attgagattg agtcttgcca 420 atccttcttt ggaagttgac aatttaaaaa ttcgaagcca gatttactcc taattaatta 480 ttgagcttga agtcttaatt accgattaca tctctgagca gtagtagtcg atgatctttc 540 ccggtcacta attttactca agcgaatcga tttttacttt agtttagcta accgtacgcc 600 attacagttt agagagcagc aagggtaaga acattattaa aacataaagt aaacctctca 660 tttattacgg tgtagtgcta attacattag cctcaaacat cctaagctta cagttttata 720 aaacacacac gatgactgtg tatagtatta acatacatca atagcaacac tcatgatcat 780 cgtgctaggc cgggcttgga ctgggtgacc taagtgatta agctaactac tcaaagcgca 840 ttcgttggct catagacaaa ggcagcatct tcagtctcaa gatcaatcct cggatcattt 900 ctcagagcat taagacgcct caggtaacat gcaccacgca aaatctgaag gttgatctgg 960 acaacacatg ggtactcatt agagacagca ggatcaagat atgttccttt gatgaagtca 1020 ttgaaggctc caatggctgg accacaccag acttgatagt ccatgacacg atcaggagca 1080 cccatgttgg cccaacgtga tgcaagacca agataccatc tgaaacacaa agacatcttg 1140 agtttggggt catgttccgc tctctgtatc ttctctggat tcttcaaacc attgatgtaa 1200 aagtccttgg tctcttccca gacctcttga agtgccctct tgaagatcct cttctcgatc 1260 ctttcaagtt cagcaggagg cattgaatcg aaagagtcgt acttgcaaaa gagttcgtac 1320 aatttgttag ctcttgatgg gaacattgtt cccttcttga gaacttggag tttcacacct 1380 tcctcaaaca tatcagctgc tggagccatg caaatgtcac tgtaggtggc ttgactgagt 1440 tgttttctca cgttgtcaca tgtcccggac tgtttagcaa cttgattgac agtcccagtc 1500 actatgaagg cagctcccat tgtgagggca gccgcagcag cttgtgggca gccaacaccg 1560 cctccagctc caactctgac tctaaggtgc gcaggataac cacactctct gtgcagtctg 1620 ttcctaaggt tgatgatcaa aggcaagatg acgtggatgg gacggttgtc agtgtgacca 1680 ccagaatcag cttcaacagc gatgtcgtct gcgacaggaa cacgacgggc aagttcggct 1740 tgttcttgtg ttatctcacc agaagctatg agtttctcaa ggaggtgttc cggagccggt 1800 ctgatgaaca tttcggcgag ttctgttctg gacacttttc caatgatacg gtttctgatg 1860 ttgacactcc catcagcgtt ccttgagagt ccggctgcac ggtaacgaac aacttgggga 1920 gtgagggtca tgaatgcaga agcctccacc acagtaacgc ccttttcgag gaagagatca 1980 acattcccct tttccaagtt gctgtcaaaa ggtgaatgaa tgaggttcac agcatatggt 2040 ccttgaggca gagccgcttg tatcttctcc aaagctgccc tcacatggtg cattggaagc 2100 ccacccgctc caaaagaacc caatatcttc ctcttcccag cagcaatgac aaggtccgca 2160 gacgcaattc ccttggccat tgctccagtg tacagaggtg ctgacacatt gtacgtttcc 2220 atgaaggact tgtcaccaag atcacttatg gagcatggtt tgatactgac aatccttgaa 2280 gaagttgcag gtttggattc aaactgcacc aaattggagt tttcaacacc aagatacaat 2340 ggattgtcca cttcaagcag gacatctctc aactgagtcg gactgagtgc aactccagtt 2400 ttcccagggt ccgcttttgc gctgggtggc tgttgttgaa catccggaat ggcttgaggt 2460 tttgcactga caacacgaac tctgagttct ttggcttcat acacacggag accatcaacc 2520 caaagtgctc catctgccac tatgtcaacg tagcctccat tttcaggaga ggctgtgatt 2580 gaaactatgt gcacctcaca gtccatcgca cggttctttg gtgtcaattg accacggtac 2640 ttccacgccg tttttccagg ggcatgggca aaggtcggat tgctcacatt gtattctccc 2700 ggtatgttct ggtccaccgc aaatgcctca atgagctgga acattgactc aattcccaaa 2760 gatccaggca tcacaggatc aaaccaaaag tgacaagaga agaaccagtc ttgtgggtta 2820 acatctttgt gtccatgcgc atatcctttc ccatgcagcc cagaatcagg agcaatgttg 2880 aggtaatcca aatactcaca ctgttctgac cttctgagaa cttgagcatt agttgcatct 2940 ggaagtttct cagtcagctg ttctttgcct ttggctgatg cgatttcaac tcttctcaca 3000 ctcgtgttgt tagttttgca ccaaggctcc gtctttttcc cattgtcaag tccagcttgc 3060 tgagcaaaca cctctggagt gaaccatcca aaggaagtgc ttcctttgta gaaaaccacg 3120 ccatcaacag acaactcaaa cgtgaatctg tgaatcccca tctttcccaa cattgcatat 3180 ccagtgcact tggtcacatt ccttatggtt ttccccctca catccacatc tggcctcacc 3240 atttcagcac ttgcatcaag gttcctaaag agaatgtcat ctttgtccat cgtcagtggt 3300 gctttcaaca cactggtgag aacaccggat gtctgaagtc cgatttccat gatgatggaa 3360 taaggcatgt gcccatcatt gcaagaacca tcaaagaacc acgcatcttg aggacagtca 3420 aactccccca ccattgtgcc tttgctggga ttgcaatcaa cattcaggaa cttgccatgt 3480 tccatgtttg tgacttcaac aactctagtc acaagagcca aatcaaaagc agggcttctg 3540 ctcgtatttg acttatcaaa tcttgcgaac tctggtccca agcagttgct aacttttcca 3600 cacatgaact ctgacatgtt gtaccagttc agaggcattt ctccagcttt gcaattgttg 3660 tcaagtgggt tgccagggaa agggatgaag caaattgccc tcggagggta agaactaggg 3720 gagaagaacg gcgtcgggtt gccaggaagt tttgacatgg gatgccagga catttctttc 3780 ggagcgagag cgtcaccctt gaggatatgt tcaggaagtg caactggctt tggcgcaaga 3840 acatctgctg agaaagcagg cttcgcaggg gcagcagaag cactagcact ggcaccagag 3900 ctggccttgg acgcttccgg tcccacagtg gctgcaccgt tagcaaaaac gggctgaact 3960 ttggaggggt tcttgttcgt ggatctcttc tgcatcttga gagcaatgcc cttgaagtcc 4020 acgacgatct ttttgttaag atcccccttt ccgtagtcgc tgattctatc aaggctgaaa 4080 tcttgaccct tttcgaaatc aacgtcaatg atgttgacgt cagcaatggc gtacggatcg 4140 ttatcctcgt cgaatcccat ctctttgatc tccatgacgt agaccaactt tcccttatga 4200 ggagatatct gacccctaca cctaactttg ttcgggtgtc cgttgacggg tctgaagtca 4260 aatggtccgg tggtaaggtg caaaccgagc cagatcatgt acatcttgag catttgtgaa 4320 caaccgtcgc tgacaagaga tccagccatg acttgatcct taacaaaatg gcatgggaag 4380 taccaatggt ccctctccaa gattttctca ccaaccaact gacccaaacc gtacacacct 4440 cccttatggt caatgctggt gactctatca atcatgagca tcttacgtgc gcagagtttg 4500 tagttgatct ccggcatgcc attctttgag ccaaaaacgg aagcccaatc tttgtcaacc 4560 aaggtctgca tctccttttc gttgagtttt gttttgtgaa ggcagggagc gacagcatag 4620 ggagaaacat cttgctttgg aatcttagct ctggcagcca aatcccctct tgtgaacacc 4680 acaccctttc ccgcatcaag ctcctcattt gtgaagaagc cagcacagcc atctctcatc 4740 tcaatgagaa gcctcccatt gacatagcag tcatactcaa agaagaacat ggagatgcca 4800 ccatccaaac gtttggcaaa accggtcact ctgatgtcat actcaagggt ctctccctca 4860 tgggcaaccc catagaatgt caacgtggtg ttgagcagac ggtaaactct gtcaccttgg 4920 ttctggaaat caattcccat gtatgatatc agcatcaaat cgcactgtcc actctcaacc 4980 agaactgccc agggacaatc acctccctca gacaattcgc cattcactgg aagatcatac 5040 tcagtgacca ttctggcacc aactctgtag ttgttgacct cggcatccat gagtgtgacc 5100 cttgtcacaa gcaggtattc acgagcaggc agcctcaccc ttctggggta cttgtctatg 5160 acagcaaact ccggaccaaa aaccttagct atgtcaccct ctgcaaactc caggagttcc 5220 tcatagttga acacctcagt tttgccagag gtcagttcct ccttggtcat ctcccaacat 5280 ggcttcttgt tggtcttgac acgaagcgcc atttttggtt gtggatccgt tttgctattt 5340 gtgtatgttt tcttgttttg gtttgagttt aaccatcatt ctcttctctc tatttataga 5400 gagaagaggt tggcacgcag cactgcgaca tgagagaaag acacgggtga agaggcggga 5460 gctgaggtgg cagcatgcat gcatgcatgg ccagtcgtgt catagtgaga agaggagaaa 5520 gctgaggtgg caacgtgtat ggccagtggt gtcatagtgt gaaagctgac gtggcagcat 5580 gcatggtgag tgtgaaaaag gcgccacagt gcagatgtta ctgaacaaaa gcggtgtgcc 5640 atgcaacttt tgcccatcgt cgtctttctc atccaagttc ctctttataa tatcttttac 5700 caaatcatat tttaatataa attcatttta tttttaaata aagtaatatt atatccataa 5760 atattaactc tcatttaaca cattttcaaa tttaacactt ctgtaaataa atttattaac 5820 aaaaacttgt aaatatgttc aaataaatcc ataaatatta actttcattt tattaacaaa 5880 aaaatcttta ctataagctg cggccgctta attaagatga gtgatactca ggactcagga 5940 ctcactctgc tgatcactag tgctagcctc gaggtcgaca caactttgta tacaaaagtt 6000 gtctaggatc tgcttttaca tgcgagacac atcttctaaa gtaattttaa taatagttac 6060 tatattcaag atttcatata tcaaatactc aatattactt ctaaaaaatt aattagatat 6120 aattaaaata ttactttttt aattttaagt ttaattgttg aatttgtgac tattgattta 6180 ttattctact atgtttaaat tgttttatag atagtttaaa gtaaatataa gtaatgtagt 6240 agagtgttag agtgttaccc taaaccataa actataagat ttatggtgga ctaattttca 6300 tatatttctt attgctttta ccttttcttg gtatgtaagt ccgtaactgg aattactgtg 6360 ggttgccatg acactctgtg gtcttttggt tcatgcatgg atcttgcgca agaaaaagac 6420 aaagaacaaa gaaaaaagac aaaacagaga gacaaaacgc aatcacacaa ccaactcaaa 6480 ttagtcactg gctgatcaag atcgccgcgt ccatgtatgt ctaaatgcca tgcaaagcaa 6540 cacgtgctta acatgcactt taaatggctc acccatctca acccacacac aaacacattg 6600 cctttttctt catcatcacc acaaccacct gtatatattc attctcttcc gccacctcaa 6660 tttcttcact tcaacacacg tcaacctgca tatgcgtgtc atcccatgcc caaatctcca 6720 tgcatgttcc aaccaccttc tctcttatat aatacctata aatacctcta atatcactca 6780 cttctttcat catccatcca tccagagtac tactactcta ctactataat accccaaccc 6840 aactcatatt caatactact ctaggatcca caaccaaaaa tggctgcaag gaacgtgagt 6900 gcggctcatg agatgcatga cgagaagagg atagctgttg tgggcatggc tgttcagtat 6960 gctggatgca aaacgaaaga cgagttctgg gaagttctca tgaatggaaa agttgagagc 7020 aaagtgatca gcgacaaacg tcttggttca aattaccgtg ctgaacacta caaagctgaa 7080 aggagcaagt atgctgacac cttctgcaat gagacatatg gaactcttga tgagaacgaa 7140 atagacaatg agcatgaact ccttttgaac cttgccaaac aagccttggc tgaaacatct 7200 gtcaaagatt caacaagatg tggcatagtt tctggatgcc tcagcttccc gatggacaac 7260 ctccaaggtg aactcttgaa tgtgtaccag aaccatgttg agaagaaact tggtgccaga 7320 gttttcaaag atgcctctca ctggtcagaa agagaacagt ccaacaaacc agaggctggt 7380 gacagaagaa tcttcatgga ccctgcttcc tttgttgctg aggaactcaa tcttggcgct 7440 ctccactact ctgttgatgc tgcctgtgcc actgcgctct atgtgctcag actggcgcaa 7500 gatcacttgg tctctggtgc agctgatgtg atgctttgtg gtgcaacatg cctgccggag 7560 ccgtttttca tactctctgg gttctcaacc ttccaagcca tgcctgttgg aacgggtcag 7620 aatgtgtcca tgccgctgca caaagattct caaggattga cccctggtga aggaggttcc 7680 atcatggtcc tcaaacgttt ggatgacgca atcagagatg gagatcacat ctatggaaca 7740 cttttgggag ccaatgtcag caacagtggc actggtctgc cacttaaacc ccttttgccg 7800 tcagagaaaa agtgcctcat ggacacctac acacgtatca atgtgcatcc ccacaagatt 7860 cagtatgttg aatgccatgc aactgggact ccacaaggtg atcgtgtgga aattgatgct 7920 gtgaaggctt gctttgaagg caaagtccca cgttttggaa ccacaaaagg gaactttgga 7980 cacacattgg tggcagctgg gtttgctggc atgtgcaaag tgcttctctc catgaagcac 8040 gggatcattc cacccactcc gggaatagat gacgaaacga agatggaccc tctggttgtc 8100 tctggtgagg ccattccctg gccagaaacg aatggagaac cgaagcgtgc tggtctctca 8160 gcgtttggat ttggtgggac caatgctcat gctgtctttg aggaacatga cccaagcaat 8220 gctgcatgca ctggccatga ctccatttct gctctctctg cacgttgtgg tggtgagtca 8280 aacatgagaa tagcaatcac tgggatggat gccacttttg gtgctttgaa aggattggat 8340 gcttttgaaa gggccatcta cactggtgct catggtgcta taccactccc agaaaagaga 8400 tggagattcc tggggaaaga caaggacttc cttgatcttt gtggagtcaa agcaacaccg 8460 catggctgtt acattgaaga tgttgaagtt gatttccaga ggctgaggac tccaatgact 8520 cctgaagata tgctcttgcc tcaacagctt ctggctgtca ccacaattga tagggccata 8580 ttggactctg gcatgaagaa aggtggaaat gtggctgtct ttgtggggtt ggggaccgat 8640 cttgaactct accgtcaccg tgcaagggtt gctctcaagg agagagtgag gcctgaagcc 8700 agcaagaaac tcaatgacat gatgcagtac atcaatgatt gtgggacaag cacttcttac 8760 accagctaca taggcaactt ggttgcaaca agggtcagtt cacaatgggg cttcacggga 8820 ccttccttca caatcacaga aggcaacaat tctgtgtaca gatgtgctga acttggcaaa 8880 tacctcttgg aaacgggaga ggttgatggg gttgtggttg cgggtgttga tctctgtggg 8940 agtgctgaga acctttacgt caagtcaagg cgtttcaagg tgtctacatc tgacaccccg 9000 agagccagct ttgatgcagc tgctgatggt tactttgttg gagagggctg tggtgcattt 9060 gtgctcaagc gtgagacaag ctgcaccaag gatgacagaa tctatgcttg catggatgcc 9120 atagttcctg gaaatgttcc atctgcatgt ttgagagagg cattggatca agccagagtc 9180 aagcctggag acatcgagat gttggagctt tctgctgatt cagcgaggca tttgaaggac 9240 ccgtctgttc tcccaaagga actcactgct gaggaagaga ttggcggact tcagacgatt 9300 cttcgtgacg atgacaagct cccaaggaat gttgcaactg gaagtgtcaa ggccactgtt 9360 ggtgacactg gttatgcttc tggtgctgcc agtttgatta aggctgcgct ttgcatctac 9420 aaccgttacc tgcccagcaa tggagatgac tgggatgaac ctgcacctga agcaccctgg 9480 gacagcacac tctttgcgtg ccagacctca agagcttggt tgaagaaccc tggtgagaga 9540 cgttatgcgg ctgtttccgg agtcagtgaa accagaagtt gctacagtgt gctgctttct 9600 gaagcagagg gacactacga gagagagaac agaatcagtt tggatgagga agcacccaag 9660 ctcattgtgt tgagagcaga ctcccatgaa gagattcttg gaagattgga caagataagg 9720 gaaaggttct tgcagcccac gggtgctgca cccagagagt ctgaattgaa ggcgcaagcc 9780 cgtaggatct ttcttgaact ccttggtgag acccttgccc aagatgcagc ttcatctgga 9840 agtcagaagc cccttgctct cagcttggtc tccactccat ccaagctcca gagagaggtg 9900 gaacttgcgg caaaaggcat tccgagatgt ctcaagatga gacgtgattg gagttcccct 9960 gctggaagca gatatgcgcc tgagcctttg gccagtgaca gagttgcctt catgtatgga 10020 gaaggaagga gcccttacta tggcatcaca caagacattc ataggatttg gcctgaactt 10080 catgaggtca tcaatgagaa aacaaaccgt ctctgggctg aaggtgacag atgggtcatg 10140 ccaagggcca gcttcaagag tgagcttgag tcacaacagc aagagtttga cagaaacatg 10200 attgaaatgt tccgtcttgg aatcctcacc tcaattgcct tcacaaacct ggcgagagat 10260 gttctcaaca tcacgcccaa agcagccttt ggactcagtt tgggagagat ttccatgatc 10320 tttgctttct ccaagaaaaa cggtttgata agcgatcagt tgacaaagga tttgagagag 10380 tcagatgtct ggaacaaagc tctggctgtt gagttcaatg cgctcagaga agcctggggc 10440 attccacagt ctgtcccaaa agatgagttc tggcaaggtt acatagtgag gggaacgaaa 10500 caagacatag aagcagccat agcaccagac tcaaagtatg tgaggttgac catcatcaat 10560 gatgccaaca ctgctctcat cagtggaaag cctgatgcat gcaaagctgc gattgctcgt 10620 cttggtggca acatccctgc gcttccagtc actcaaggca tgtgtgggca ttgcccagag 10680 gtgggacctt acaccaagga catcgcaaag atccacgcca accttgagtt ccctgtggtt 10740 gatggtcttg atctctggac taccataaac cagaagaggc ttgtgccaag agccacgggt 10800 gccaaagatg aatgggctcc ttcatccttt ggggagtatg ctggacagct ctatgagaaa 10860 caagccaact tccctcagat agttgaaacc atctacaagc agaactatga tgtgtttgtt 10920 gaagttgggc caaacaatca cagaagcaca gcagtgagaa ccacgcttgg accacagaga 10980 aaccaccttg ctggtgccat agacaagcag aacgaggatg cttggacgac aatagtcaag 11040 ctcgtggctt cattgaaggc tcaccttgtt cctggagtga ccatcagtcc gctctaccac 11100 tccaagctcg ttgcagaggc tgaggcttgt tatgctgcgc tctgtaaagg tgagaaaccc 11160 aagaaaaaca agtttgtgag aaagattcag ttgaatggca gattcaacag caaggcagac 11220 cccatctcct cagcagacct tgcatccttc ccacctgcgg accctgcgat tgaagctgcc 11280 atctcttcaa ggatcatgaa gcctgtggct ccgaaattct atgcgcgtct caacattgat 11340 gagcaagatg agaccagaga tccgatcttg aacaaggaca atgcgccttc cagctccagc 11400 tcttccagca gttcctcttc cagcagttca tctccgtctc ctgctccttc agctccagtt 11460 cagaagaaag ctgccccagc tgcggagaca aaagctgttg ccagtgctga tgcacttcgt 11520 agtgccctgc ttgatcttga ctccatgctt gcgctcagct ctgcaagtgc ttctggcaac 11580 cttgttgaga ctgcgccatc tgatgccagt gtcattgtcc ctccgtgcaa cattgctgat 11640 cttggcagca gagctttcat gaaaacttat ggtgtttctg cgcctctgta caccggtgca 11700 atggccaagg gaattgcctc tgcggacttg gtgatcgcag ctggaaggca aggaatcctt 11760 gcgtcatttg gtgctggagg ccttccaatg caagttgtgc gtgagtccat tgaaaagatt 11820 caagctgccc tgcccaatgg accatatgct gtcaacctca tccattctcc ttttgacagc 11880 aaccttgaga agggaaatgt tgatctcttc ttggaaaagg gtgtcacttt tgttgaggct 11940 tcagccttca tgacgttgac accgcaagtt gtcagataca gagcggctgg gttgacgagg 12000 aatgcagatg ggtcagtcaa cataaggaac agaatcattg ggaaagtctc cagaactgag 12060 ctggctgaaa tgttcatgcg tcctgcacct gagcatcttt tgcagaagct cattgcttct 12120 ggggagatca atcaagaaca agctgagttg gccagacgtg ttcctgtggc tgatgacatt 12180 gcggttgaag ctgattctgg tggacacact gacaaccgtc caatccatgt cattctgcct 12240 ctgattatca acctgaggga taggcttcac agagagtgtg gatacccagc aaacctgagg 12300 gttcgtgttg gtgctggggg tggcattggg tgtccccaag ctgcgctggc aactttcaac 12360 atgggtgcct ctttcattgt cactggaaca gtgaaccaag ttgccaaaca atctgggacg 12420 tgtgacaatg tgaggaaaca gcttgcgaaa gcaacttact cagatgtttg catggcaccg 12480 gcagctgaca tgtttgagga aggtgtgaaa cttcaagttt tgaagaaagg aacaatgttc 12540 ccttcaagag ccaacaaact ctatgagttg ttctgcaagt atgattcttt tgagtccatg 12600 cctccagcag agcttgcgag ggttgagaaa agaatcttca gtcgtgcatt ggaagaggtc 12660 tgggatgaga ccaagaactt ctacatcaac cgtctccaca acccagagaa gatacagagg 12720 gctgagaggg accccaaact gaagatgtca ctgtgtttca gatggtatct gtccctggct 12780 tcaaggtggg ccaacactgg agcttctgac agagtcatgg actaccaagt ttggtgtggt 12840 cctgccattg gttcattcaa tgatttcatc aaaggaactt accttgatcc ggcagtggca 12900 aatgagtacc cgtgtgttgt gcagatcaac aaacagattc ttagaggagc gtgcttcttg 12960 aggcgtcttg aaatactgag aaatgcaagg ctttcagatg gtgcagctgc acttgtggcg 13020 agcattgatg acacatatgt ccctgctgag aagctgtgag tagttagctt aatcacttag 13080 gtcacctcga gtatcaaaat ctatttagaa atacacaata ttttgttgca ggcttgctgg 13140 agaatcgatc tgctatcata aaaattacaa aaaaatttta tttgcctcaa ttattttagg 13200 attggtatta aggacgctta aattatttgt cgggtcacta cgcatcattg tgattgagaa 13260 gatcagcgat acgaaatatt cgtagtacta tcgataattt atttgaaaat tcataagaaa 13320 agcaaacgtt acatgaattg atgaaacaat acaaagacag ataaagccac gcacatttag 13380 gatattggcc gagattactg aatattgagt aagatcacgg aatttctgac aggagcatgt 13440 cttcaattca gcccaaatgg cagttgaaat actcaaaccg ccccatatgc aggagcggat 13500 cattcattgt ttgtttggtt gcctttgcca acatgggagt ccaaggttgg cgcgccgaat 13560 tcgcggccgc ttaattaact actgtcactg aggccgtaga cgagtacgga ctgatctaac 13620 tagtgctagc ctcgaggtcg acacccagct ttcttgtaca aagtggttgc ggccgcttaa 13680 ttaaatttaa attcaattaa tgcaatcttg attttcaaca acgaaggtaa tggcgtaaaa 13740 gaaaaaatgt atgttattgt attgatcttt catgatgttg aagcgtgcca taatatgatg 13800 atgtataatt aaaatattaa ctgtcgcatt ttattgaaat ggcactgtta tttcaaccat 13860 atctttgatt ctgttacatg acacgactgc aagaagtaaa taatagacgc cgttgttaaa 13920 gaattgctat catatgtgcc taactagagg gaatttgagc gtcagaccta atcaaatatt 13980 acaaaatatc tcactctgtc gccagcaatg gtgtaatcag cgcagacaaa tggcgtaaag 14040 atcgcggaaa aacctccccg agtggcatga tagctgcctc tgtattgctg atttagtcag 14100 ccttatttga cttaagggtg ccctcgttag tgacaaattg ctttcaagga gacagccatg 14160 ccccacactt tgttgaaaaa caaattgcct ttggggagac ggtaaagcca gttgctcttc 14220 aataaggaat gtcgaggagg caatgtaacc gcctctggta gtacacttct ctaatccaaa 14280 aatcaatttg tattcaagat accgcaaaaa acttatggtt taaaccctgc aggactagtc 14340 cagaaggtaa ttatccaaga tgtagcatca agaatccaat gtttacggga aaaactatgg 14400 aagtattatg taagctcagc aagaagcaga tcaatatgcg gcacatatgc aacctatgtt 14460 caaaaatgaa gaatgtacag atacaagatc ctatactgcc agaatacgaa gaagaatacg 14520 tagaaattga aaaagaagaa ccaggcgaag aaaagaatct tgaagacgta agcactgacg 14580 acaacaatga aaagaagaag ataaggtcgg tgattgtgaa agagacatag aggacacatg 14640 taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca aaggaatctt atcccccact 14700 acttatcctt ttatattttt ccgtgtcatt tttgcccttg agttttccta tataaggaac 14760 caagttcggc atttgtgaaa acaagaaaaa atttggtgta agctattttc tttgaagtac 14820 tgaggataca acttcagaga aatttgtaag tttgtaggta ccagatctgg atcccaaacc 14880 atgtctccgg agaggagacc agttgagatt aggccagcta cagcagctga tatggccgcg 14940 gtttgtgata tcgttaacca ttacattgag acgtctacag tgaactttag gacagagcca 15000 caaacaccac aagagtggat tgatgatcta gagaggttgc aagatagata cccttggttg 15060 gttgctgagg ttgagggtgt tgtggctggt attgcttacg ctgggccctg gaaggctagg 15120 aacgcttacg attggacagt tgagagtact gtttacgtgt cacataggca tcaaaggttg 15180 ggcctaggat ctacattgta cacacatttg cttaagtcta tggaggcgca aggttttaag 15240 tctgtggttg ctgttatagg ccttccaaac gatccatctg ttaggttgca tgaggctttg 15300 ggatacacag cccggggtac attgcgcgca gctggataca agcatggtgg atggcatgat 15360 gttggttttt ggcaaaggga ttttgagttg ccagctcctc caaggccagt taggccagtt 15420 acccaaatct gagtagttag cttaatcacc tagagctcga tcggcggcaa tagcttctta 15480 gcgccatccc gggttgatcc tatctgtgtt gaaatagttg cggtgggcaa ggctctcttt 15540 cagaaagaca ggcggccaaa ggaacccaag gtgaggtggg ctatggctct cagttccttg 15600 tggaagcgct tggtctaagg tgcagaggtg ttagcgggat gaagcaaaag tgtccgattg 15660 taacaagata tgttgatcct acgtaaggat attaaagtat gtattcatca ctaatataat 15720 cagtgtattc caatatgtac tacgatttcc aatgtcttta ttgtcgccgt atgtaatcgg 15780 cgtcacaaaa taatccccgg tgactttctt ttaatccagg atgaaataat atgttattat 15840 aatttttgcg atttggtccg ttataggaat tgaagtgtgc ttgaggtcgg tcgccaccac 15900 tcccatttca taattttaca tgtatttgaa aaataaaaat ttatggtatt caatttaaac 15960 acgtatactt gtaaagaatg atatcttgaa agaaatatag tttaaatatt tattgataaa 16020 ataacaagtc aggtattata gtccaagcaa aaacataaat ttattgatgc aagtttaaat 16080 tcagaaatat ttcaataact gattatatca gctggtacat tgccgtagat gaaagactga 16140 gtgcgatatt atggtgtaat acataggaat tcgtttaaac gatctgcgtc taattttcgg 16200 tccaacttgc acaggaaaga cgtcgaccgc ggtagctctt gcccagcaga ctgggcttcc 16260 agtcctttcg ctcgatcggg tccaatgttg tcctcagctg tgaaccggaa gcggacgacc 16320 aacagtggaa gaactgaaag gaacgagccg tctatacctt gatgatcggc ctctggtgaa 16380 gggtatcatc gcagccaagc aagctcatga aaggctgatg ggggaggtgt ataattatga 16440 ggcccacggc gggcttattc tttagggagg atctatctcg ttgctcaagt gcatggcgca 16500 aagcagttat tggagtgcgg attttcgttg gcatattatt cgccacgagt tagcagacga 16560 agagaccttc atgaacgtgg ccaaggccag agttaagcag atgttacgcc ctgctgcagg 16620 cctttctatt atccaatagt tggttgatct ttggaaagag cctcggctga ggcccatact 16680 gaaagagatc gatggatatc gatatgccat gttgtttgct agccagaacc agatcacatc 16740 cgatatgcta ttgcagcttg acgcagatat ggaggataag ttgattcatg ggatcgctca 16800 ggagtagctc atccatgcac gccgacaaga acagaaattc cgtcgagtta acgcagccgc 16860 ttacgacgga ttcgaaggtc atccattcgg aatgtattag tttgcaccag ctccgcgtca 16920 cacctgtctt catttgaata agatgttagc aattgttttt agctttgtct tgttgtggca 16980 gggcggcaag tgcttcagac atcattctgt tttcaaattt tatgctggag aacagcttct 17040 taattccttt ggaaataata gactgcgtct taaaattcag atgtctggat atagatatga 17100 ttgtaaaata acctatttaa gtgtcattta gaacataagt tttatgaatg ttcttccatt 17160 ttcgtcatcg aacgaataag agtaaataca ccttttttaa cattacaaat aagttcttat 17220 acgttgttta tacaccggga atcatttcca ttattttcgc gcaaaagtca cggatattcg 17280 tgaaagcgac ataaactgcg aaatttgcgg ggagtgtctt gagtttgcct cgaggctagc 17340 gcatgcacat agacacacac atcatctcat tgatgcttgg taataattgt cattagattg 17400 tttttatgca tagatgcact cgaaatcagc caattttaga caagtatcaa acggatgtga 17460 cttcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgatt 17520 tacaattgaa tatatcctgc cccagccagc caacagctcg atttacaatt gaatatatcc 17580 tgccggccgg cccacgcgtg tcgaggaatt ctgatctggc ccccatttgg acgtgaatgt 17640 agacacgtcg aaataaagat ttccgaatta gaataatttg tttattgctt tcgcctataa 17700 atacgacgga tcgtaatttg tcgttttatc aaaatgtact ttcattttat aataacgctg 17760 cggacatcta catttttgaa ttgaaaaaaa attggtaatt actctttctt tttctccata 17820 ttgaccatca tactcattgc tgatccatgt agatttcccg gacatgaagc catttacaat 17880 tgaatatatc ctgccgccgc tgccgctttg cacccggtgg agcttgcatg ttggtttcta 17940 cgcagaactg agccggttag gcagataatt tccattgaga actgagccat gtgcaccttc 18000 cccccaacac ggtgagcgac ggggcaacgg agtgatccac atgggacttt taaacatcat 18060 ccgtcggatg gcgttgcgag agaagcagtc gatccgtgag atcagccgac gcaccgggca 18120 ggcgcgcaac acgatcgcaa agtatttgaa cgcaggtaca atcgagccga cgttcacgcg 18180 gaacgaccaa gcaagcttgg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg 18240 cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg 18300 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt 18360 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc 18420 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt 18480 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt 18540 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca 18600 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc 18660 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt 18720 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga 18780 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg 18840 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg 18900 tcggaaaggg tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga 18960 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg 19020 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac 19080 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga 19140 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg 19200 ctgatgaaac caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag 19260 acgaacgaag agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc 19320 tgctggccgt cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg 19380 agctggcccg catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca 19440 ccgacgaccc gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga 19500 tcgaagagaa gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag 19560 agccatgact tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc 19620 cccatgcgct ccatcaagaa gagcgacttc gcggagctgg tattcgtgca gggcaagatt 19680 cggaatacca agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat 19740 aaggtggatt atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt 19800 gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg 19860 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc 19920 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc 19980 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc 20040 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg 20100 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc 20160 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag 20220 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg 20280 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc 20340 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc 20400 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag 20460 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag 20520 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc 20580 ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg 20640 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg 20700 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag 20760 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg 20820 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc 20880 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg 20940 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg 21000 tcagttccgg ctgggggttc agcagccagc gctttactgg catttcagga acaagcgggc 21060 actgctcgac gcacttgctt cgctcagtat cgctcgggac gcacggcgcg ctctacgaac 21120 tgccgataaa cagaggatta aaattgacaa ttgtgattaa ggctcagatt cgacggcttg 21180 gagcggccga cgtgcaggat ttccgcgaga tccgattgtc ggccctgaag aaagctccag 21240 agatgttcgg gtccgtttac gagcacgagg agaaaaagcc catggaggcg ttcgctgaac 21300 ggttgcgaga tgccgtggca ttcggcgcct acatcgacgg cgagatcatt gggctgtcgg 21360 tcttcaaaca ggaggacggc cccaaggacg ctcacaaggc gcatctgtcc ggcgttttcg 21420 tggagcccga acagcgaggc cgaggggtcg ccggtatgct gctgcgggcg ttgccggcgg 21480 gtttattgct cgtgatgatc gtccgacaga ttccaacggg aatctggtgg atgcgcatct 21540 tcatcctcgg cgcacttaat atttcgctat tctggagctt gttgtttatt tcggtctacc 21600 gcctgccggg cggggtcgcg gcgacggtag gcgctgtgca gccgctgatg gtcgtgttca 21660 tctctgccgc tctgctaggt agcccgatac gattgatggc ggtcctgggg gctatttgcg 21720 gaactgcggg cgtggcgctg ttggtgttga caccaaacgc agcgctagat cctgtcggcg 21780 tcgcagcggg cctggcgggg gcggtttcca tggcgttcgg aaccgtgctg acccgcaagt 21840 ggcaacctcc cgtgcctctg ctcaccttta ccgcctggca actggcggcc ggaggacttc 21900 tgctcgttcc agtagcttta gtgtttgatc cgccaatccc gatgcctaca ggaaccaatg 21960 ttctcggcct ggcgtggctc ggcctgatcg gagcgggttt aacctacttc ctttggttcc 22020 gggggatctc gcgactcgaa cctacagttg tttccttact gggctttctc agcccccgag 22080 cgcttagtgg gaatttgtac cccttatcga accgggagca caggatgacg cctaacaatt 22140 cattcaagcc gacaccgctt cgcggcgcgg cttaattcag gagttaaaca tcatgaggga 22200 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca 22260 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa 22320 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg 22380 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct 22440 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc 22500 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga 22560 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt 22620 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt 22680 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga 22740 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc 22800 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt 22860 catacttgaa gctaggcagg cttatcttgg acaagaagat cgcttggcct cgcgcgcaga 22920 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata 22980 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag 23040 agagctgggg aagactatgc gcgatctgtt gaaggtggtt ctaagcctcg tcttgcgatg 23100 gcatttcgat ccattcccat tccgcgctca agatggcttc ccctcggcag ttcatcaggg 23160 ctaaatcaat ctagccgact tgtccggtga aatgggctgc actccaacag aaacaatcaa 23220 acaaacatac acagcgactt attcacacga gctca 23255 <210> 71 <211> 8 <212> PRT <213> Artificial sequence <220> <223> ORFA Reference Protein 1 <400> 71 Leu Asn Tyr Val Val Val Glu Lys 1 5 <210> 72 <211> 14 <212> PRT <213> Artificial sequence <220> <223> ORFA Reference Protein 2 <400> 72 Phe Gly Ala Leu Gly Gly Phe Ile Ser Gln Gln Ala Glu Arg 1 5 10 <210> 73 <211> 39 <212> PRT <213> Artificial sequence <220> <223> ORFA Reference Protein 3 <400> 73 Ala Glu Ile Ala Gly Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro 1 5 10 15 Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala Pro Ala Pro Ala Val 20 25 30 Ser Ser Glu Leu Leu Glu Lys 35 <210> 74 <211> 17 <212> PRT <213> Artificial sequence <220> <223> ORFA Reference Protein 4 <400> 74 Ala Ala Pro Ala Ala Ala Ala Pro Ala Val Ser Asn Glu Leu Leu Glu 1 5 10 15 Lys <210> 75 <211> 18 <212> PRT <213> Artificial sequence <220> <223> ORFA Reference Protein 5 <400> 75 Ile Val Gln His Arg Pro Val Pro Gln Asp Lys Pro Phe Tyr Ile Thr 1 5 10 15 Leu Arg <210> 76 <211> 8 <212> PRT <213> Artificial sequence <220> <223> ORFA Reference Protein 6 <400> 76 Ile Phe Val Glu Phe Gly Pro Lys 1 5 <210> 77 <211> 24 <212> PRT <213> Artificial sequence <220> <223> apo form of pantetheinylation sites 2-9 <400> 77 Thr Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr 1 5 10 15 Glu Leu Gly Ile Asp Ser Ile Lys 20 <210> 78 <211> 24 <212> PRT <213> Artificial sequence <220> <223> apo form of pantetheinylation site 1 <400> 78 Thr Gly Tyr Glu Thr Asp Met Ile Glu Ala Asp Met Glu Leu Glu Thr 1 5 10 15 Glu Leu Gly Ile Asp Ser Ile Lys 20

Claims (68)

  1. (i) 적어도 하나의 다중불포화 지방산(polyunsaturated fatty acid; PUFA)을 생산하는 PUFA 신타제(synthase) 시스템을 인코딩하는 핵산 서열 및
    (ii) 포스포판테테이닐 보조인자를 PUFA 신타제 시스템 ACP 도메인으로 전달하는 포스포판테테이닐 트랜스퍼라제(PPTase)를 인코딩하는 핵산 서열을 포함하는, 유전자 변형된 브라시카(Brassica) 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  2. 제1항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 브라시카 나푸스(Brassica napus)인, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  3. 제1항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 브라시카 준세아(Brassica juncea)인, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  4. 제1항 내지 제3항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 1의 아미노산 서열과 적어도 80% 동일한 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  5. 제1항 내지 제3항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 1의 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  6. 제1항 내지 제3항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이, 서열번호 6의 핵산 서열과 적어도 80% 동일한 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  7. 제1항 내지 제3항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이 서열번호 6의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  8. 제1항 내지 제7항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 2의 아미노산 서열과 적어도 80% 동일한 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  9. 제1항 내지 제7항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 2의 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  10. 제1항 내지 제7항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이, 서열번호 7의 핵산 서열과 적어도 80% 동일한 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  11. 제1항 내지 제7항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이 서열번호 7의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  12. 제1항 내지 제11항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 3의 아미노산 서열과 적어도 80% 동일한 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  13. 제1항 내지 제11항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 3의 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  14. 제1항 내지 제11항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이, 서열번호 8의 핵산 서열과 적어도 80% 동일한 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  15. 제1항 내지 제11항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이 서열번호 8의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  16. 제1항 내지 제3항 중의 어느 한 항에 있어서, PUFA 신타제 시스템이 서열번호 1 내지 3의 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 종자 또는 부분.
  17. 제1항 내지 제3항 중의 어느 한 항에 있어서, PUFA 신타제 시스템을 인코딩하는 핵산 서열이 서열번호 6 내지 8의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  18. 제1항 내지 제17항 중의 어느 한 항에 있어서, PPTase가 서열번호 5와 적어도 80% 동일한 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  19. 제1항 내지 제17항 중의 어느 한 항에 있어서, PPTase가 서열번호 5의 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  20. 제1항 내지 제17항 중의 어느 한 항에 있어서, PPTase를 인코딩하는 핵산 서열이 서열번호 10의 핵산 서열과 적어도 80% 동일한, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  21. 제1항 내지 제17항 중의 어느 한 항에 있어서, PPTase를 인코딩하는 핵산 서열이 서열번호 10의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  22. 제1항 내지 제21항 중의 어느 한 항에 있어서, (i) 및 (ii)의 핵산 서열이 단일 재조합 발현 벡터에 함유되어 있는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  23. 제1항 내지 제22항 중의 어느 한 항에 있어서, (i) 및 (ii)의 핵산 서열이 종자 특이적 프로모터에 작동적으로 연결되어 있는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  24. 제1항 내지 제23항 중의 어느 한 항에 있어서, (i) 및 (ii)의 핵산 서열이 PvDlec2, LfKCS3 및 FAE 1로 이루어진 그룹으로부터 선택된 프로모터에 작동적으로 연결되어 있는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  25. 제1항 내지 제24항 중의 어느 한 항에 있어서,
    (iii) 장쇄 PUFA 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신세타제(ACoAS)를 인코딩하는 핵산 서열을 추가로 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  26. 제25항에 있어서, ACoAS가 서열번호 4와 적어도 80% 동일한 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  27. 제25항에 있어서, ACoAS가 서열번호 4의 아미노산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  28. 제25항에 있어서, ACoAS를 인코딩하는 핵산 서열이 서열번호 9의 핵산 서열과 적어도 80% 동일한 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  29. 제25항에 있어서, ACoAS를 인코딩하는 핵산 서열이 서열번호 9의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  30. 제25항에 있어서, ACoAS를 인코딩하는 핵산 서열이 서열번호 34의 핵산 서열을 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  31. 제25항 내지 제30항 중의 어느 한 항에 있어서, (i), (ii) 및 (iii)의 핵산 서열이 단일 재조합 발현 벡터에 함유되어 있는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  32. 제25항 내지 제31항 중의 어느 한 항에 있어서, (i), (ii) 및 (iii)의 핵산 서열이 종자 특이적 프로모터에 작동적으로 연결되어 있는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  33. 제25항 내지 제32항 중의 어느 한 항에 있어서, (i), (ii) 및 (iii)의 핵산 서열이 PvDlec2, LfKCS3 및 FAE 1로 이루어진 그룹으로부터 선택된 프로모터에 작동적으로 연결되어 있는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  34. 제1항 내지 제33항 중의 어느 한 항에 있어서, 아세틸 CoA 카복실라제(ACCase)를 인코딩하는 핵산 서열 및/또는 타입 2 디아실글리세롤 아실트랜스퍼라제(DGAT2)를 인코딩하는 핵산 서열을 추가로 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  35. 서열번호 6 내지 10으로부터 선택된 핵산 서열을 포함하는 분리된 핵산 분자.
  36. 재조합 발현 벡터 pDAB7361.
  37. 재조합 발현 벡터 pDAB7362.
  38. 재조합 발현 벡터 pDAB7363.
  39. 제1항 내지 제34항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 검출가능한 양의 DHA(도코사헥사엔산(C22:6, n-3)), DPA(C22:5, n-6 또는 n-3), 또는 EPA(에이코사펜타엔산(C20:5, n-3))를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  40. 제1항 내지 제34항 및 제39항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.01% 내지 15% DHA를 포함하는, 유전자 변형된 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  41. 제1항 내지 제34항, 제39항 및 제40항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.05% 내지 10% DHA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  42. 제1항 내지 제34항 및 제39항 내지 제41항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.05% 내지 5% DHA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  43. 제1항 내지 제34항 및 제39항 내지 제42항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.01% 내지 5% EPA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  44. 제1항 내지 제34항 및 제39항 내지 제43항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.05% 내지 5% EPA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  45. 제1항 내지 제34항 및 제39항 내지 제44항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.05% 내지 1% EPA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  46. 제1항 내지 제34항 및 제39항 내지 제45항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.01% 내지 5% DPA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  47. 제1항 내지 제34항 및 제39항 내지 제46항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.05% 내지 5% DPA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  48. 제1항 내지 제34항 및 제39항 내지 제47항 중의 어느 한 항에 있어서, 식물, 이의 후대, 세포, 조직, 종자 또는 부분이 0.05% 내지 1% DPA를 포함하는, 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분.
  49. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분으로부터 수득된 오일.
  50. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 종자,.
  51. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분으로부터 수득된 종자 오일 (seed oil).
  52. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분으로부터 수득된 오일, 또는 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 종자를 포함하는 식료품(food product).
  53. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분으로부터 수득된 오일, 또는 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 종자를 포함하는 기능성 식품(functional food).
  54. 제1항 내지 제34항 및 제39항 내지 제45항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분으로부터 수득된 오일, 또는 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분으로부터 수득된 종자를 포함하는 약품(pharmaceutical product).
  55. 제1항 내지 제34항 및 제39항 내지 제45항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분으로부터, 또는 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분의 종자로부터 오일을 회수하는 단계를 포함하는, 적어도 하나의 PUFA를 포함하는 오일을 생산하는 방법.
  56. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분을 성장시키는 단계를 포함하는, 적어도 하나의 PUFA를 포함하는 오일을 생산하는 방법.
  57. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분의 종자로부터 오일을 회수하는 단계를 포함하는, 종자 오일에서 적어도 하나의 PUFA를 생산하는 방법.
  58. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분을 성장시키는 단계를 포함하는, 종자 오일에서 적어도 하나의 PUFA를 생산하는 방법.
  59. 제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분, 제49항의 오일, 제50항의 종자, 제51항의 종자 오일, 제52항의 식료품, 제53항의 기능성 식품 또는 제54항의 약품을 개체에게 제공하는 단계를 포함하는, 적어도 하나의 PUFA를 함유하는 보충 또는 치료 생성물을 개체에게 제공하는 방법.
  60. 제55항 내지 제59항 중의 어느 한 항에 있어서, PUFA가 DHA인 방법.
  61. 제55항 내지 제59항 중의 어느 한 항에 있어서, PUFA가 EPA인 방법.
  62. 브라시카 식물 또는 식물 세포를,
    (i) 적어도 하나의 다중불포화 지방산(PUFA)을 생산하는 조류 PUFA 신타제를 인코딩하는 핵산 서열 및 (ii) 포스포판테테이닐 보조인자를 조류 PUFA 신타제 ACP 도메인으로 전달하는 포스포판테테이닐 트랜스퍼라제(PPTase)를 인코딩하는 핵산 서열로 형질전환시키는 단계를 포함하는,
    제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직 또는 부분을 생산하는 방법.
  63. 제62항에 있어서, 브라시카 식물 또는 식물 세포를, (iii) 장쇄 PUFA 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신세타제(ACoAS)를 인코딩하는 핵산 서열로 형질전환시키는 단계를 추가로 포함하는 방법.
  64. 약 0.05% 내지 5% DHA를 포함하는 브라시카 식물의 종자로부터의 오일 조성물.
  65. 약 0.05% 내지 5% DHA를 포함하고 70% 초과 올레산(중량 기준)의 지방산 프로파일을 갖는 브라시카 식물의 종자로부터의 오일 조성물.
  66. 약 0.05% 내지 5% DHA를 포함하고 3.5% 미만 알파-리놀렌산(중량 기준)의 지방산 프로파일을 갖는 브라시카 식물의 종자로부터의 오일 조성물.
  67. 약 0.05% 내지 5% DHA를 포함하며 70% 초과 올레산 및 3.5% 미만 알파-리놀렌산(중량 기준)의 지방산 프로파일을 갖는 브라시카 식물의 종자로부터의 오일 조성물.
  68. 브라시카 식물 또는 식물 세포를,
    (i) 적어도 하나의 다중불포화 지방산(polyunsaturated fatty acid; PUFA)을 생산하는 PUFA 신타제 시스템을 인코딩하는 핵산 서열 및 (ii) 장쇄 PUFA 유리 지방산(FFA)의 아실-CoA로의 전환을 촉매하는 아실-CoA 신세타제(ACoAS)를 인코딩하는 핵산 서열, 및 (iii) PUFA 신타제 시스템 ACP 도메인을 활성화시키는 포스포판테테이닐 트랜스퍼라제(PPTase)를 인코딩하는 핵산 서열을 포함하는 발현 카세트로 형질전환시키는 단계를 포함하는,
    제1항 내지 제34항 및 제39항 내지 제48항 중의 어느 한 항의 유전자 변형된 브라시카 식물, 이의 후대, 세포, 조직, 종자 또는 부분을 생산하는 방법.
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