KR20130094714A - 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법 - Google Patents

비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법 Download PDF

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KR20130094714A
KR20130094714A KR1020127030167A KR20127030167A KR20130094714A KR 20130094714 A KR20130094714 A KR 20130094714A KR 1020127030167 A KR1020127030167 A KR 1020127030167A KR 20127030167 A KR20127030167 A KR 20127030167A KR 20130094714 A KR20130094714 A KR 20130094714A
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sample
polymerase
nucleic acid
detecting
activity
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Korean (ko)
Inventor
숀 마크 오'하라
다니엘 알. 쯔웨잇직
Original Assignee
제우스 사이언티픽, 아이엔씨.
오＇하라, 샤운, 마크
즈웨이지그, 다니엘, 알.
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Application filed by 제우스 사이언티픽, 아이엔씨., 오＇하라, 샤운, 마크, 즈웨이지그, 다니엘, 알. filed Critical 제우스 사이언티픽, 아이엔씨.
Publication of KR20130094714A publication Critical patent/KR20130094714A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020127030167A 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법 Ceased KR20130094714A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US32494910P 2010-04-16 2010-04-16
US32493910P 2010-04-16 2010-04-16
US61/324,939 2010-04-16
US61/324,949 2010-04-16
US32541310P 2010-04-19 2010-04-19
US61/325,413 2010-04-19
PCT/US2011/032600 WO2011130584A2 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Related Child Applications (1)

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KR1020187021294A Division KR102068136B1 (ko) 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법

Publications (1)

Publication Number Publication Date
KR20130094714A true KR20130094714A (ko) 2013-08-26

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KR1020127030167A Ceased KR20130094714A (ko) 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법
KR1020187021294A Active KR102068136B1 (ko) 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법

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KR1020187021294A Active KR102068136B1 (ko) 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법

Country Status (16)

Country Link
US (3) US20130196318A1 (https=)
EP (3) EP3617320B1 (https=)
JP (2) JP2013537399A (https=)
KR (2) KR20130094714A (https=)
CN (1) CN103328652B (https=)
AP (1) AP2012006530A0 (https=)
AU (2) AU2011239587B2 (https=)
CA (1) CA2796676C (https=)
EA (1) EA201291001A1 (https=)
ES (3) ES2644258T5 (https=)
MY (1) MY161567A (https=)
NZ (2) NZ701866A (https=)
PH (2) PH12012502065A1 (https=)
SG (2) SG10201502900PA (https=)
WO (1) WO2011130584A2 (https=)
ZA (1) ZA201208522B (https=)

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US20130196318A1 (en) 2010-04-16 2013-08-01 Shawn Mark O'Hara Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US9708651B2 (en) * 2012-01-05 2017-07-18 Zeus Scientific, Inc. DNA polymerase activity assays and methods enabling determination of viable microbes
CN104520438A (zh) * 2012-04-12 2015-04-15 宙斯科技公司 用于任何聚合酶延伸活性的灵敏的,定量的聚合酶活性检测和用于确定活细胞的存在的方法
GB201412316D0 (en) * 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230112608A1 (en) 2021-10-13 2023-04-13 Disruptive Oil And Gas Technologies Corp Nanobubble dispersions generated in electrochemically activated solutions
CN114032285B (zh) * 2021-11-24 2022-05-24 北京擎科生物科技有限公司 PolyA聚合酶活性检测方法
US20250191691A1 (en) * 2023-12-07 2025-06-12 California Institute Of Technology Methods for the translated alignment of transcriptomic data at single-cell resolution
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
SE462497B (sv) * 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
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Also Published As

Publication number Publication date
EP3284831B1 (en) 2019-07-24
NZ720529A (en) 2017-01-27
SG184563A1 (en) 2012-11-29
CA2796676C (en) 2020-07-14
EP3284831A1 (en) 2018-02-21
JP6603956B2 (ja) 2019-11-13
AU2011239587B2 (en) 2016-03-10
ZA201208522B (en) 2014-08-27
WO2011130584A2 (en) 2011-10-20
EP2558600A2 (en) 2013-02-20
US10876173B2 (en) 2020-12-29
EA201291001A1 (ru) 2013-09-30
US10870894B2 (en) 2020-12-22
CN103328652A (zh) 2013-09-25
SG10201502900PA (en) 2015-06-29
KR102068136B1 (ko) 2020-01-20
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
EP2558600B1 (en) 2017-10-04
US20180230551A1 (en) 2018-08-16
US20170321287A1 (en) 2017-11-09
PH12012502065A1 (en) 2019-07-10
EP3617320A1 (en) 2020-03-04
PH12016500300A1 (en) 2019-02-04
AU2016203878A1 (en) 2016-06-30
EP2558600A4 (en) 2014-04-30
KR20180086526A (ko) 2018-07-31
CA2796676A1 (en) 2011-10-20
CN103328652B (zh) 2016-12-28
NZ701866A (en) 2016-07-29
ES2906061T3 (es) 2022-04-13
EP3617320B1 (en) 2022-01-26
EP2558600B2 (en) 2020-06-17
AP2012006530A0 (en) 2012-10-31
JP2013537399A (ja) 2013-10-03
US20130196318A1 (en) 2013-08-01
MY161567A (en) 2017-04-28
JP2017148067A (ja) 2017-08-31
WO2011130584A3 (en) 2013-07-11
AP3635A (https=) 2016-03-09
ES2739806T3 (es) 2020-02-04
AU2011239587A1 (en) 2012-11-08

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