CA2796676C - Methods for measuring enzyme activity useful in determining cell viability in non-purified samples - Google Patents
Methods for measuring enzyme activity useful in determining cell viability in non-purified samples Download PDFInfo
- Publication number
- CA2796676C CA2796676C CA2796676A CA2796676A CA2796676C CA 2796676 C CA2796676 C CA 2796676C CA 2796676 A CA2796676 A CA 2796676A CA 2796676 A CA2796676 A CA 2796676A CA 2796676 C CA2796676 C CA 2796676C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- microorganism
- polymerase
- dna
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32494910P | 2010-04-16 | 2010-04-16 | |
| US32493910P | 2010-04-16 | 2010-04-16 | |
| US61/324,939 | 2010-04-16 | ||
| US61/324,949 | 2010-04-16 | ||
| US32541310P | 2010-04-19 | 2010-04-19 | |
| US61/325,413 | 2010-04-19 | ||
| PCT/US2011/032600 WO2011130584A2 (en) | 2010-04-16 | 2011-04-15 | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2796676A1 CA2796676A1 (en) | 2011-10-20 |
| CA2796676C true CA2796676C (en) | 2020-07-14 |
Family
ID=44799329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2796676A Active CA2796676C (en) | 2010-04-16 | 2011-04-15 | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US20130196318A1 (https=) |
| EP (3) | EP3284831B1 (https=) |
| JP (2) | JP2013537399A (https=) |
| KR (2) | KR102068136B1 (https=) |
| CN (1) | CN103328652B (https=) |
| AP (1) | AP2012006530A0 (https=) |
| AU (2) | AU2011239587B2 (https=) |
| CA (1) | CA2796676C (https=) |
| EA (1) | EA201291001A1 (https=) |
| ES (3) | ES2906061T3 (https=) |
| MY (1) | MY161567A (https=) |
| NZ (2) | NZ701866A (https=) |
| PH (2) | PH12012502065A1 (https=) |
| SG (2) | SG10201502900PA (https=) |
| WO (1) | WO2011130584A2 (https=) |
| ZA (1) | ZA201208522B (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102068136B1 (ko) | 2010-04-16 | 2020-01-20 | 모멘텀 바이오사이언스, 리미티드 | 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법 |
| KR20150005511A (ko) | 2012-01-05 | 2015-01-14 | 제우스 사이언티픽, 아이엔씨. | 개선된 dna 중합효소 활성 분석 및 생육성 미생물의 검출을 가능하게 하는 방법 |
| JP2015513921A (ja) * | 2012-04-12 | 2015-05-18 | ゼウス サイエンティフィック、インク． | 任意のポリメラーゼ伸長活性の高感度定量的測定と生細胞存在の決定とに有用なポリメラーゼ活性測定方法 |
| GB201412316D0 (en) * | 2014-07-10 | 2014-08-27 | Momentum Bioscience Ltd | Detecting the absence of micro-organisms |
| GB2528647A (en) * | 2014-07-10 | 2016-02-03 | Momentum Bioscience Ltd | Detecting viable microorganisms |
| US9879328B2 (en) * | 2014-11-21 | 2018-01-30 | GeneWeave Biosciences, Inc. | Mechanisms of antimicrobial susceptibility |
| US10662484B1 (en) | 2016-03-24 | 2020-05-26 | Bench in a Box, LLC | Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria |
| GB201805479D0 (en) * | 2018-04-03 | 2018-05-16 | Momentum Bioscience Ltd | Microorganism separation and detection |
| GB201914538D0 (en) | 2019-10-08 | 2019-11-20 | Momentum Bioscience Ltd | Microorganism capture from antimicrobial-containing solution |
| US20230112608A1 (en) | 2021-10-13 | 2023-04-13 | Disruptive Oil And Gas Technologies Corp | Nanobubble dispersions generated in electrochemically activated solutions |
| CN114032285B (zh) * | 2021-11-24 | 2022-05-24 | 北京擎科生物科技有限公司 | PolyA聚合酶活性检测方法 |
| US20250191691A1 (en) * | 2023-12-07 | 2025-06-12 | California Institute Of Technology | Methods for the translated alignment of transcriptomic data at single-cell resolution |
| GB202405229D0 (en) | 2024-04-12 | 2024-05-29 | Momentum Bioscience Ltd | Microorganism enumeration |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS501953B1 (https=) | 1968-08-05 | 1975-01-22 | ||
| JPS505942B1 (https=) | 1970-08-23 | 1975-03-08 | ||
| JPS6054697A (ja) * | 1983-09-05 | 1985-03-29 | イザド−ラ ブロドスキイ | 診断方法および装置 |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| SE462497B (sv) † | 1988-11-30 | 1990-07-02 | Clas Fredrik Runesson Kaelland | Saett foer att aktivitetsbestaemma polymeraser |
| DE69028725T2 (de) * | 1989-02-28 | 1997-03-13 | Canon Kk | Partiell doppelsträngiges Oligonukleotid und Verfahren zu seiner Bildung |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| DE4027616A1 (de) * | 1990-08-31 | 1992-03-05 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung von polymerase-aktivitaet |
| JPH06509477A (ja) | 1992-05-11 | 1994-10-27 | シュープバッハ・イエルク | 逆転写酵素の検出方法 |
| US5489508A (en) † | 1992-05-13 | 1996-02-06 | University Of Texas System Board Of Regents | Therapy and diagnosis of conditions related to telomere length and/or telomerase activity |
| US5629154A (en) † | 1993-11-12 | 1997-05-13 | Geron Corporation | Telomerase activity assays |
| JPH0622798A (ja) * | 1992-07-07 | 1994-02-01 | Hitachi Ltd | 塩基配列決定法 |
| US5719028A (en) * | 1992-12-07 | 1998-02-17 | Third Wave Technologies Inc. | Cleavase fragment length polymorphism |
| US5863726A (en) † | 1993-11-12 | 1999-01-26 | Geron Corporation | Telomerase activity assays |
| ATE215608T1 (de) | 1994-07-13 | 2002-04-15 | Secr Defence Brit | Mikrobiologisches testverfahren und reagenzien |
| US6054278A (en) * | 1997-05-05 | 2000-04-25 | The Perkin-Elmer Corporation | Ribosomal RNA gene polymorphism based microorganism identification |
| WO2001007643A1 (en) * | 1999-07-28 | 2001-02-01 | Kaken Pharmaceutical Co., Ltd. | Method for detecting pathogenic microorganism and antimicrobial agent, method for evaluating the drug effect of antimicrobial agent, and antimicrobial agents |
| GB9923846D0 (en) | 1999-10-09 | 1999-12-08 | Quip Technology Limited | Reverse transcriptase assay |
| EP1529117A2 (en) * | 2002-08-08 | 2005-05-11 | University of Lausanne | Method for the rapid assessment of the presence and viability of bacterial cells and use thereof |
| US20050136439A1 (en) * | 2003-09-12 | 2005-06-23 | North Carolina State University | Novel methods of inorganic compound discovery and synthesis |
| GB0508981D0 (en) * | 2005-05-03 | 2005-06-08 | Acolyte Biomedica Ltd | Distinguishing cells in a sample |
| WO2006123154A2 (en) * | 2005-05-18 | 2006-11-23 | Iseao Technologies Limited | Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid |
| EP1963528A4 (en) * | 2005-12-20 | 2009-11-25 | Ming-Sheng Lee | APPARATUS, METHODS AND PRODUCTS FOR DETECTION OF GENETIC MUTATION |
| US7947447B2 (en) * | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| CA2692800A1 (en) * | 2007-07-09 | 2009-01-15 | Christopher John Stanley | Detection of micro-organisms based on their nad-dependent dna ligase activity |
| KR102068136B1 (ko) | 2010-04-16 | 2020-01-20 | 모멘텀 바이오사이언스, 리미티드 | 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법 |
-
2011
- 2011-04-15 KR KR1020187021294A patent/KR102068136B1/ko active Active
- 2011-04-15 JP JP2013505162A patent/JP2013537399A/ja active Pending
- 2011-04-15 WO PCT/US2011/032600 patent/WO2011130584A2/en not_active Ceased
- 2011-04-15 AP AP2012006530A patent/AP2012006530A0/xx unknown
- 2011-04-15 EP EP17194583.5A patent/EP3284831B1/en active Active
- 2011-04-15 SG SG10201502900PA patent/SG10201502900PA/en unknown
- 2011-04-15 US US13/641,480 patent/US20130196318A1/en not_active Abandoned
- 2011-04-15 NZ NZ701866A patent/NZ701866A/en not_active IP Right Cessation
- 2011-04-15 MY MYPI2012004567A patent/MY161567A/en unknown
- 2011-04-15 ES ES19187658T patent/ES2906061T3/es active Active
- 2011-04-15 EP EP19187658.0A patent/EP3617320B1/en active Active
- 2011-04-15 CA CA2796676A patent/CA2796676C/en active Active
- 2011-04-15 EA EA201291001A patent/EA201291001A1/ru unknown
- 2011-04-15 EP EP11769633.6A patent/EP2558600B2/en active Active
- 2011-04-15 CN CN201180019406.9A patent/CN103328652B/zh active Active
- 2011-04-15 AU AU2011239587A patent/AU2011239587B2/en not_active Ceased
- 2011-04-15 NZ NZ720529A patent/NZ720529A/en not_active IP Right Cessation
- 2011-04-15 ES ES17194583T patent/ES2739806T3/es active Active
- 2011-04-15 PH PH1/2012/502065A patent/PH12012502065A1/en unknown
- 2011-04-15 SG SG2012076097A patent/SG184563A1/en unknown
- 2011-04-15 KR KR1020127030167A patent/KR20130094714A/ko not_active Ceased
- 2011-04-15 ES ES11769633T patent/ES2644258T5/es active Active
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2012
- 2012-11-13 ZA ZA2012/08522A patent/ZA201208522B/en unknown
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2015
- 2015-06-12 US US14/737,736 patent/US10870894B2/en active Active
- 2015-08-12 US US14/825,092 patent/US10876173B2/en active Active
-
2016
- 2016-02-12 PH PH12016500300A patent/PH12016500300A1/en unknown
- 2016-06-09 AU AU2016203878A patent/AU2016203878A1/en not_active Abandoned
-
2017
- 2017-04-25 JP JP2017086485A patent/JP6603956B2/ja active Active
Also Published As
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| Publication | Publication Date | Title |
|---|---|---|
| CA2796676C (en) | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples | |
| JP2013537399A5 (https=) | ||
| EP2179052B1 (en) | Detection of micro-organisms based on their nad-dependent dna ligase activity | |
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| Class et al. | Patent application title: Methods for Detection of Micro-Organisms Inventors: Christopher John Stanley (Cambridge, GB) Stuart Wilson (London, GB) Assignees: MICROSEN MEDTECH LIMITED |
Legal Events
| Date | Code | Title | Description |
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Effective date: 20160408 |
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