KR20120063646A - A processing method of liquid by using salmon testis extract for anti-aging and anti-oxidant care - Google Patents

Abstract

PURPOSE: A method for manufacturing a cosmetic functional sap using salmon testis is provided to ensure antibacterial and moisturizing effect. CONSTITUTION: A method for manufacturing a cosmetic functional sap for antiaging and antioxidation comprises: a step of separating and collecting testis from a salmon; a step of washing the testis with alkali electrolyzed water and sterilizing with acid electrolyzed water; a step of dipping the salmon testis in 70-95% spirit for 20-30 minutes and drying; a step of hydrolyzing the salmon testis by enzyme and freeze-drying(S13); and a step of mixing 0.1-1.0 wt% of freeze-dried salmon testis, 92.094.9 wt% of deep seawater, and 5.0-7.0 wt% of natural crude drug extract and stirring(S14).

Description

A method for producing anti-aging and antioxidant functional sap for cosmetics using salmon testis {A PROCESSING METHOD OF LIQUID BY USING SALMON TESTIS EXTRACT FOR ANTI-AGING AND ANTI-OXIDANT CARE}

The present invention relates to a method for preparing cosmetic anti-aging and anti-oxidant sap by stirring the salmon into a proper ratio of deep sea water and natural herbal extracts extracted from nucleic acids and protamine extracted from salmon testis as a by-product.

Skin is the largest organ in the human body, its size is about 1.6㎡, and it is a large organ that weighs 16% of its weight by weight. The skin serves as the most basic skin covering the body to protect internal organs. In addition, it is a very important institution that performs many functions.

The skin is constantly in contact with changes in the external environment such as ultraviolet rays, temperature changes, moisture, dust, and air pollution, and it is important to protect the individual by quickly reading the information essential for survival and allowing the body to react appropriately. In charge of the task, it has an additional function to protect the skin itself to some extent from external harm.

However, as we get older, our skin cannot deny the inherent loss of its own function, and in order to supplement it, we have to borrow the power of cosmetics following the consumption of food.

The close association between aging and oxidation is well known in many studies.

In particular, it is known that wrinkling caused by aging of the skin and blackening phenomena such as local spots and blemishes are correlated with this oxidation phenomenon. And genetic factors due to the shape of the muscles and the constant relaxation of the muscles. Furthermore, it can be divided into external factors due to environmental pollution and stress.

Skin aging caused by various factors such as keratin production, decreased fibroblast division, decreased collagen synthesis, decreased hyaluronic acid production, increased signal transduction of melanin production, wrinkles, blemishes and freckles And blemishes gradually increase.

In order to improve such skin aging, AHA, retinoic acid, retinol, ascorbic acid and the like have been mainly used to apply the herbal extracts containing the antioxidant polyphenols.

According to the research results of fish testes, the main components of fish testes are nucleic acid, protein, and most of protamine. Nucleic acid has cell regeneration, immune function improvement, lipid peroxidation inhibition, and protamine has antimicrobial effect and electron donating ability against Gram-positive bacteria. It is done.

Nucleic acid and protamine, which are widely distributed in salmon testis, are essential proteins for our body and are important components of antioxidant enzymes that protect cells from free radicals generated during normal oxygen metabolism.

This can be said to be an essential ingredient for the normal functioning of the immune system. Therefore, if it is extracted and purified and emulsified from salmon, a byproduct of salmon, it can be absorbed into the body through the skin. The research could be more active.

In addition, researches and product launches of natural cosmetics, which are made by mixing natural herbal extracts and antibacterial functional additives to help wrinkle improvement, skin elasticity and whitening, are actively underway.

Currently, functional cosmetics in Korea are categorized into three categories: Wrinkle improvement products, whitening products, and UV protection products recognized by the Korea Food and Drug Administration through the review of functional cosmetics. As a product for improving functional anti-wrinkle with the addition of an antioxidant functional composition, it is being developed and marketed and relatively expensive products are the mainstream.

Therefore, the present invention manufactures a sap that can give anti-aging and antioxidant functionality using the deep seawater and natural herbal extracts from nucleic acids and protamine extracted from salmon testicles, so as to replenish skin moisture, elasticity and whitening, which are insufficient with age. The purpose is to provide the product to meet the tastes of the general consumers who need antibacterial and moisturizing skin as well as the elderly age group, as well as mass production and distribution sales.

The present invention is a pre-treatment step of halving the salmon to collect the whole body portion of the salmon body and separate and collect the testis part, washing in alkaline electrolytic water and sterilization in acid electrolytic water; A drying step of immersing the pretreated salmon testis in 70-95% alcohol for 20-30 minutes and then drying at 10-20% moisture content; Lyophilization step of pulverizing dried salmon testis and then hydrolyzing with enzyme and concentrating by freeze drying; A stirred manufacturing process of mixing and stirring 0.1-1.0 wt% of lyophilized salmon testis, 92.0-94.9 wt% of deep seawater, and 5.0-7.0 wt% of the natural herbal extract; It is characterized by providing a method for producing a cosmetic anti-aging and antioxidant functional sap comprising a.

The present invention is characterized in that the washing process in the alkaline electrolytic water in the pretreatment step is soaked for 5-15 minutes in the pH 10.5-11.5 strong alkaline electrolytic water of 2-5 times the weight of salmon testis.

The present invention is characterized in that sterilizing and disinfecting the acidic electrolyzed water in the pretreatment step is to immerse the salmon testes in alkaline electrolyzed water for 5-15 minutes in pH 2.5-3.5 strongly acidic electrolyzed water of 2-5 times the weight of salmon testis. .

The present invention is a natural herbal extract in the stirring manufacturing process, black soybean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract It is characterized by mixing at least one or more of them.

The black soybean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract of each of the black bean, motherwort extract, situation Mushroom, root, red ginseng, Centella, pomegranate, golden, Astragalus, Pogongyoung, Bokbunja, red ginseng and alcohol, 95% alcohol in a ratio of 1: 5 ~ 1: 20 by weight, sealed in a sealed glass container, 4-10 degrees Let the primary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the seed, pour out the extract, leaching the first black bean, motherwort, situation mushroom, brown root, red ginseng, centella, pomegranate , Golden, yellow, pogongyoung, bokbunja, red ginseng and alcoholic alcohol 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed glass container sealed to avoid the temperature and sunlight of 4-10 degrees Addition of secondary leaching for 3-8 weeks in cold dark Pour out the extract, and leach and extract the second black bean, motherwort, situation mushroom, brown root, red ginseng, centella, pomegranate, golden, Astragalus, pogongyoung, bokbunja, red ginseng and pH2.5-3.5 strongly acidic electrolyzed water. After mixing at a ratio of 1: 60 ~ 1: 90, black beans, motherwort, situation mushroom, brown root, red ginseng, Centella, pomegranate, golden, Astragalus, pogongyoung, bokbunja, red ginseng and water at the temperature of 100 ~ 120 ℃ This is the ratio of 1: 20 ~ 1: 30 so that it can be extracted 3 times by boiling water for 4 ~ 6 hours, followed by the extract, and each black soybean, motherwort, situation mushroom, brown root, red ginseng, bottle grass extracted 3 times with strong acid water bath. , Pomegranate, Golden, Astragalus, Pogongyoung, Bokbunja, Red ginseng and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60 ~ 1: 90 to the weight of each black bean, motherwort at a temperature of 100 ~ 120 ℃ , Sichuan Mushroom, Brown Root, Ginseng, Centella, Pomegranate, Golden, Astragalus, Pogongyoung, Bokbunja, Red Ginseng and Water 1: 20 ~ 1 4 to 6 hours in a ratio of: 30 to be extracted in the fourth step to extract the extract, and then extract the extract extracted over each of the four times from the above using a rotary rotary evaporator at a temperature of 10-40 degrees Celsius Concentrate the extract and extract for 10-40 minutes in a ratio of 1: 10 ~ 1: 40 to weight, respectively, black bean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract is characterized in that it produces.

The present invention produces anti-aging and antioxidant functional sap by mixing and stirring marine deep water and natural herbal extracts with nucleic acid and protamine extracted from salmon testis, and thus improving the awareness of anti-aging and antioxidant skin care that Koreans have neglected. The moisture absorption of the functional fluid application is easy for anyone to understand and immediately check for proper skin anti-aging and antioxidant recovery, maintenance and adaptation.

1 is a flow chart showing the manufacturing process of the anti-aging and antioxidant functional sap for cosmetics according to an embodiment of the present invention.

The technical problem achieved by the above-described embodiment of the present invention will be more clearly understood by preferred embodiments of the present invention described below.

The following examples are merely illustrated to illustrate the present invention and are not intended to limit the scope of the present invention.

Hereinafter, an embodiment of the present invention will be described in detail.

The present invention is a pre-treatment step (S11) to halve the salmon to collect the whole body portion of the salmon body, separate and collect the testis part, washing in alkaline electrolytic water and sterilization in acidic electrolytic water; A drying step of immersing the pretreated salmon testis in 70-95% alcohol for 20-30 minutes and then drying it at a water content of 10-20% (S12); A freeze-drying step of grinding the dried salmon testes, hydrolyzing using an enzyme, concentrating and freeze-drying (S13); It consists of a stirring manufacturing step (S14) of mixing and stirring the lyophilized salmon testament 0.1-1.0% by weight, deep sea water 92.0-94.9% by weight and natural herbal extract 5.0-7.0% by weight and stirring.

In addition, a sealing packaging step (S15) of sealing the sap prepared in the container in the container; may be added.

In particular, a method for preparing an sap that can give anti-aging and antioxidant functionality using marine deep water and natural herbal extracts to nucleic acids and protamine extracted from salmon testes according to an embodiment of the present invention is primarily alkaline in testis salmon testes. It is immersed in the electrolytic water immersion liquid for 5-15 minutes to wash and wash, and soaked in acidic electrolytic water immersion liquid for 5-15 minutes to disinfect. Therefore, it is necessary to prepare electrolytic water for the production of immersion liquid. The same amount of strongly acidic electrolytic water and strongly alkaline electrolyzed water are collected, and all of the collected strong electric seawater can be effectively utilized by preparing an immersion liquid for each purpose.

In more detail, the above-described pretreatment process (S11) is a process of collecting tap water drinking water, which is a raw material, and electrolyzing it in an electrolytic cell to produce and use the produced alkaline electrolytic and acid electrolytic water as an immersion aqueous solution. It is a process of removing and washing the intestinal filth and foreign substances, which are the food parts, and collecting them.

When the water temperature of the electrolyte is increased during the generation of electrolytic water, the ionic change is activated in the diaphragm in the electrolytic cell, so that the strong acidic electrolyzed water generated at the anode not only increases the concentration of hypochlorite (HOCL), an effective chlorine that has a disinfectant disinfecting effect, but also a strong alkali produced at the cathode. Since electrolytic water has a high concentration of sodium hydroxide (NaOH) having an interfacial effect, an underwater heater, which is a heating facility, is installed in a distilled water reservoir to operate the water temperature at 35-40 degrees Celsius.

In addition, in the above-described electrolysis process, 0.1-10.0% sodium chloride (NACL) is added to distilled water, mixed and diluted to make an electrolyte solution, and then electrolyzed in a electrolyte membrane electrolyte tank in an electrolytic cell. Exhaust pipes are installed to allow the discharge of chlorine gas from the anode.

In general, electro-analysised water refers to water obtained by applying electric force to general water such as tap water or ground water.

When the anode and cathode platinum-plated titanium is put into the water and the electricity is applied through direct current, the anion dissolved in the water is collected on the (+) pole, which becomes acidic ionized water, and the cation gathers on the (-) pole to the alkaline ionized water. Occurs.

The electrolyzed water thus produced has its ionic effect for 7-8 days when exposed to air and about 3-4 weeks when stored with the lid closed.

Strong alkaline electrolytic water on the positive electrode side and strong alkaline on the negative electrode side in the process of collecting the strongly alkaline electrolytic water generated in the electrolytic water collection process and the strong acid electrolytic water for use as the strong alkaline pretreatment aqueous solution and the strong acid pretreatment aqueous solution, respectively Collect the electrolyzed water separately and put it in the sump and circulate to maintain the water temperature at 5-10 degrees Celsius by using chiller which is a cooling facility in each sump.

Underwater UV sterilizer is installed and operated in each reservoir to sterilize so that pH property does not change.

In addition, care should be taken to prevent re-contamination of each collected ionized water.

In case of acidic ionized water, it has a converging effect and constricts the skin.

Therefore, since it protects the skin slightly acidic, it is of course excellent in skin care and disinfection.

Hypochlorite Water Additive Standard by Korea Food and Drug Administration and Hypochlorite Water Food Additive Approval Standard by Japan Ministry of Health and Welfare applies the same standards and standards, according to which the component standard of Hypochlorous Acid Water is strong acidic hot water. (Also called strongly acidic hypochlorous acid) is less than pH2.7 and contains 20-60ppm of hypochlorous acid (HOCL), an effective chlorine, and non-acidic water (also called non-acidic hypochlorous acid) is pH5.0-6.5 It contains 10-30ppm of hypochlorous acid (HOCL), and 10ppm of effective chlorine has a bactericidal effect against bacteria with low disinfectant resistance, and 20-30ppm of effective chlorine has a bactericidal effect against bacteria that do not have apopoies. An effective chlorine of 60ppm has a bactericidal effect within minutes of infant bacilli.

Sodium hypochlorite water is not effective in killing follicles, but strong acid hypochlorite is a very effective fungicide for follicles, and Bacillus and clostridium have a killing effect within 3 minutes.

Hypochlorite water is characterized by a strong bactericidal and deodorizing effect but very low biotoxicity. The bactericidal power is fungus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and other nutrients, Cereus, Acidophilus, Fungi, Viruses, Heterogeneous bacteria, Salmonella It has strong bactericidal power against food poisoning such as bacteria and Vibrio bacteria, as well as all pathogenic microorganisms caused by raw water. (Source: less than 5 seconds of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and other trophic bacteria of less than 5 minutes , 1 min-2 min 30 sec, fungus 5 sec-1 min, virus less than 5 sec, antimicrobial effect of strong acidic electrolyzed water, clinical test, 37: 919-919, 1993) Salmonella and Vibrio bacteria were not detected after 120,000-200,000 bacteria at 30 seconds after treatment, Test report on sterilization ability, Korea Testing and Research Institute, 2002) Hypochlorite is a fundamental measure of sterilization The odor is weak and experimentally 80 times stronger than sodium hypochlorite (NAOCL) and practically 5-10 times stronger, so the effect is that the bactericidal power of hypochlorite water with 30 ppm chlorine concentration is hypochlorous acid with 150 ppm chlorine concentration The US FDA (Food and Drug Administration) recognizes 1/50 of sodium hypochlorite in terms of the biotoxicity of hypochlorite water as well as being superior to sodium water (a disinfectant diluted in water). It is already widely used as a substitute for chemicals such as food disinfectants and pesticides.

Hypochlorite water is already used as an optimal disinfectant for airborne poisoning due to water, food raw materials, and spray because it is free of odors and does not contain salts.

Sterilization is increased by increasing the temperature of hypochlorous acid.

Strong alkaline ionized water is generated on the opposite (-) side as much as the amount of strong acidic water produced on the hypochlorite water production device (+) side and serves as a surfactant, so strong alkaline ionized water is used as a cleaning agent, as well as chemicals such as wax and chemical fertilizer. It is also used as a substitute.

In addition, alkaline ionized water has more beneficial minerals (inorganic salts) than normal water, and makes water molecules smaller, so the absorption of minerals is increased, helping diarrhea, constipation, and stomach action.

Strongly alkaline ionized water is a natural emulsifier with excellent dissolving and detergency, as well as a surfactant with excellent natural surfactant effect containing 40-120 ppm of sodium hydroxide (NaOH) at pH11.0 or higher. Because of its physical properties, it has a function to improve cleaning power, so it is mainly used for cleaning raw materials such as root vegetables (potatoes, potatoes, carrots, etc.), fruit vegetables (cucumbers, pumpkins, tomatoes, etc.), and grains (beans, brown rice, etc.). .

In foreign countries, strong alkaline water with a pH greater than 11.0 is recognized as a dilution solution of sodium hydroxide solution. It contains sodium hydroxide and natural emulsifiers and functions as a non-toxic natural detergent. It is due to the effect of washing off grease well.

The cleaning power is increased by using the highly alkaline ionized water at elevated temperatures.

In the case of countries where electrolyzed water is legalized by strong acid and strong alkaline ionized water, Kangsan Electrolyzed Water was announced in Korea in December 2005 by the Korea Food & Drug Administration (Packaging Container Team) No. 2005-75. It was first used as a standard and standard for hypochlorite, which is a disinfectant for facilities, and was announced as No. 2007-74 of the Korea Food and Drug Administration (Food Additives Team) in November 2007. And specifications.

Foreign cases preceded us. In June 2002, the Ministry of Health, Labor and Welfare Ordinance No. 75 and Notice No. 212 recognized strong acidic water as hypochlorous acid and approved as a food additive (sterilizer). In 1999, the sanitary water generator was approved by the Ministry of Sanitation as a disinfection device, and in 1998, the EPA (Environmental Protection Agency) approved the strong electric water generation device as a disinfectant manufacturing device in 1999, and in 1999, the FDA approved the strong acid electrolytic water. Recognized as the same as hypochlorous acid water, it was applied as a disinfectant for food materials and utensil containers, and the same year USDA (Agricultural) approved the use of electrolytic hypochlorous acid for the purpose of disinfecting intestinal pathogens of growth.

In foreign countries, since 1996, in Japan, hand pesticides, endoscope disinfection, antibacterial measures, prevention of infection in the hospital, treatment of burns, oral disinfection, and pesticide-free pesticides for crop disease control in agriculture since 1996. In 2002, it was used for cultivation and maintaining freshness of harvest.In 2002, it was used for the removal of organic odors in the environment, decomposition of pollutants, and the production of drinking water. In addition, its use has been extended to improve and preserve the quality by strong alkaline electrolyzed water.

Therefore, strong acidic electrolyzed water is a product that is approved for use in food hygiene not only in Korea but also in the world. It is harmless to humans, has no residue, and has excellent sterilizing power as can be seen from the use examples. .

As described above, the characteristics of each strong electric seawater are summarized first, and then the strong alkaline electrolytic water should be cleaned and then sterilized and disinfected with strong acidic hypochlorous acid water. If the object is first immersed in strong alkaline electrolyzed water to remove organic contaminants and washed and disinfected with strong acid electrolyzed water, the sterilization effect can be expected to increase significantly.

Therefore, in the present invention, first, by setting the order of sterilization sterilization by immersing in the strong alkaline electrolyzed water, sterilized by cleaning the salmon intestine in strong acid electrolytic water, rinsing and sterilization in parallel to maximize the effect.

In more detail, the above-described pretreatment process (S11) is a process of rinsing and disinfecting salmon by dipping the salmon internal organs, which are by-products, in distillation and alkaline electrolyzed water, washing and immersing in acidic electrolyzed water.

In general, the raw material can be any type of salmon, such as domestic coastal, Pacific, Indian, Atlantic.

In addition, salmon is a dried or specially processed biological salmon can be used both frozen and live salmon, if frozen frozen thawed.

When thawing frozen salmon, do not immerse in a cold thawing room outside the ambient temperature below 5 ° C, and use a fan for quick thawing.

Salmon consists of the head, the trunk, and the eye, bone, teeth, and intestines, which are the edible parts, so that the intestines, the intestines, are collected, separated from the testis, and collected and weighed. Washing water and dirt in flowing water, and prepared pH10.5-11.5 alkaline electrolyzed water and pH2.5-3.5 acidic electrolyzed water with immersion solution 2-5 times the weight of testes, and collecting them in an electrolytic water tank. Immerse in electrolytic water immersion tank for 5-15 minutes, and secondly immerse in acid electrolytic water immersion tank for 5-15 minutes.

In this case, for hygiene, work table, knife, cutting board, etc. should be disinfected frequently using 100 ~ 200ppm solution of chlorine or 20 ~ 25ppm solution of iodine.

The above-described drying step (S12) is a process of first drying the pretreated salmon testes at a moisture content of 10-20%.

The above-mentioned stirring preparation process (S14) is enzymatic hydrolysis and concentration of lyophilized salmon testis (nucleic acid and protamine family), deep sea water and natural herbal extract in a weight ratio of 0.1-1.0: 92.0-94.9; Mix at a rate of 5.0-7.0 and finally stir with a homogenizer for 30-45 minutes at a temperature condition of 50-70 degrees Celsius.

At this time, the salmon testis is less than 0.1 when mixing the effect is weak, when mixing more than 1.0 may cause skin irritation is preferably mixed in a ratio of 0.1-1.0.

And the natural herbal extract is black bean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract will be.

At this time, each extract is to have an antioxidant and anti-aging function, the synergistic effect is generated only when mixed in a mixing ratio of 0.1-1.0% or more.

And the black bean extract, motherwort extract, situation mushroom extract, reed root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract, each black bean, motherwort, situation mushroom, Brown, red ginseng, Centella, pomegranate, golden, yellow, pogongyoung, bokbunja, red ginseng and alcoholic alcohol 95% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container of 4-10 degrees Let the primary leaching extract for 3-8 weeks in a cool dark place avoiding temperature and sunlight, pour out the extract, and extract the black beans, motherwort, situation mushroom, brown root, red ginseng, centella, pomegranate, golden , Hwanggi, Pogongyoung, Bokbunja, Red ginseng and alcoholic beverage 25% alcohol in a ratio of 1: 5 ~ 1: 20 by weight in sealed airtight glass containers, 4-10 degrees Celsius and avoiding sunlight Leaching extraction for 3-8 weeks Pour the extract, leach, and extract the black beans, motherwort, situation mushroom, brown root, red ginseng, centella, pomegranate, golden, Astragalus, pogongyoung, bokbunja, red ginseng and pH2.5-3.5 strong acidic electrolyzed water. After mixing at a ratio of 1: 60 ~ 1: 90, black beans, motherwort, situation mushroom, brown root, red ginseng, Centella, pomegranate, golden, Astragalus, pogongyoung, bokbunja, red ginseng and water at the temperature of 100 ~ 120 ℃ This is the ratio of 1: 20 ~ 1: 30 so that it can be extracted 3 times by boiling water for 4 ~ 6 hours, followed by the extract, and each black soybean, motherwort, situation mushroom, brown root, red ginseng, bottle grass extracted 3 times with strong acid water bath , Pomegranate, Golden, Astragalus, Pogongyoung, Bokbunja, Red ginseng and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60 ~ 1: 90 to the weight of each black bean, motherwort at a temperature of 100 ~ 120 ℃ , Sichuan Mushroom, Brown Root, Ginseng, Centella, Pomegranate, Golden, Astragalus, Pogongyoung, Bokbunja, Red Ginseng and Water 1: 20 ~ 1: 30 4 ~ 6 hours in a ratio so that the extract is extracted in the 4th time to pour the extract, and the extract extracted from each of the above 4 times and mixed with the extract using a vacuum rotary evaporator at a temperature of 10-40 ° C setting conditions Concentrate for 10-40 minutes to the ratio of 1:10 ~ 1:40 to the weight of black bean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, Pogongyoung extract, Bokbunja extract, red ginseng extract will be prepared.

In the above-described configuration, the sealing packaging step (S15) is weighed and prepared by weighing the sap prepared by stirring the contents and sealed in a container to pass the packaged product through a metal detector immediately after distribution.

Each of the sap prepared above is taken to measure pH.

In this case, the container should be sterilized by air drying after immersion in the disinfectant solution using alcohol, strong acidic electrolyzed water and iodine.

In the above series of processes, in order to prevent the growth of microorganisms at room temperature, the elapsed time of the process should be made within 4 hours during the stirring packaging process and the sealing packaging and the rapid freezing process.

The following are the results of experiments with various characteristics of the sap prepared according to the comparative example and the antibacterial anti-aging and antioxidant functional sap using deep seawater and natural herbal medicine in nucleic acid and protamine extracted from salmon testis prepared according to an embodiment of the present invention Compare and review.

First, the manufacturing process of antimicrobial anti-aging and antioxidant functional sap using deep sea water and natural herbal medicines in nucleic acid and protamine extracted from salmon testis according to an embodiment of the present invention is carried out as follows.

[Pretreatment Process]

1) Collect salmon testament which is a by-product of salmon removed by halving the salmon on the workbench, and remove the foreign substance after sorting and removing it, wash it under running water, weigh 1,000g, immerse in alkaline electrolytic water for 10 minutes, wash and wash in acidic electrolytic water. Sterilize by soaking for a minute.

2) In the electrolysis process, weigh 20.0 g of tap water and 30.0 g of sodium chloride relative to the weight of distilled water in an electrolytic solution, stir it in an electrolytic cell, and then electrolyze it in a diaphragm electrolytic cell in the electrolytic cell.

An underwater heater, which is a heating facility, is installed in the alkaline electrolytic water collector generated by the cathode to operate the water temperature at 35 degrees Celsius.

10,015g strongly alkaline electrolyzed water and 10,015g strongly acidic electrolyzed water generated in the above-mentioned electrolytic water collection process are collected for use as a strong alkaline pretreatment aqueous solution and a strong acid pretreatment aqueous solution, respectively. The strong alkaline electrolytic water on the cathode side is collected separately and placed in water collecting tanks, and the water temperature is maintained at 5-10 degrees Celsius by using a chiller as a cooling facility in each water collecting tank.

Underwater UV sterilizer is installed and operated in each reservoir to sterilize so that pH property does not change.

In addition, care should be taken to prevent re-contamination of each collected ionized water.

[Drying process]

3) The pretreated salmon testes are soaked in 70-95% alcohol for 20-30 minutes and dried at 10-20% moisture content.

Even in this case, the food contact surfaces around the cold and warm air drying chambers or work benches are frequently disinfected using 70% alcohol solution.

[Freeze drying process]

4) Grind dried salmon testis, hydrolyze using enzyme, concentrate and freeze-dry.

[Stirring Manufacturing Process]

5) Moisturizing agent and functional additives are added to nucleic acid and protamine extracted from hydrolyzed and concentrated lyophilized salmon testis to prepare sap for anti-aging antioxidant improvement.

0.5 g of nucleic acid and protamine, 93.5 g of desalted seawater, 0.5 g of black soybean extract, 0.5 g of motherwort extract, 0.5 g of situation mushroom extract, 0.5 g of brown root extract, 0.5 g of red ginseng extract, 0.5 g of Centella asiatica, 0.5g Pomegranate Extract, 0.5g Golden Extract, 0.5g Astragalus Extract, 0.5g Pogongyoung Extract, 0.5g Bokbunja Extract, 0.5g Red Ginseng Extract at 70 ° C. for 35 minutes Final agitation with a homogenizer.

[Sealing packaging process]

7) Each of the sap prepared above is sorted, put in a PP container, sealed, sealed packaging, and passed through a metal detector.

8) Store the product at room temperature and distribute it at room temperature.

Hereinafter, the results of experimenting with various characteristics of the anti-aging and antioxidant functional sap for cosmetics prepared according to the embodiment of the present invention and the sap prepared according to another comparative example will be examined.

In Experimental Example 1 below, sensory tests were performed on anti-aging and antioxidant wrinkle improvement.

Experimental Example 1

0.5% by weight of nucleic acid, protamine and 99.5% by weight of purified water (Comparative Example 1), 0.5% by weight of nucleic acid, protamine and 99.5% by weight of deep sea water (Comparative Example 2), 0.5% by weight Of nucleic acid, protamine, 90.5% by weight of deep seawater and 9.0% by weight of natural herbal extract (Comparative Example 3), 0.5% by weight of nucleic acid, protamine, 97.5% by weight of deep sea water and 2.0% by weight of natural herbal medicine When the extracts were mixed (Comparative Example 4) and each sap prepared according to an embodiment of the present invention (example) applied to the skin to examine the anti-aging and antioxidant effects that occur two weeks after application A comprehensive sensory evaluayion test was conducted to improve moisturizing, improve wrinkles, improve skin shine and skin side effects.

The sensory inspectors were 20 males and females who were trained in their 20s and 40s.The 5-point scoring method (5: very strong, 4: strong, 3: normal, 2: weak, 1: very weak) was used. The sample was repeated and averaged, and the sample was applied twice a day by lightly spreading on the skin under the condition of application after washing morning and evening twice a day. Moisturizing improvement, elastic wrinkle improvement, skin shine improvement, skin side effects were evaluated and arithmetic average (see Table 1).

As a result of the experiment, as shown in Table 1 below, the effects of moisturizing, elastic wrinkles, skin shine, and skin side effects for the anti-aging and anti-oxidation improvement effect were compared to the case of using water-based pure water (Comparative Example 1). It was found to be high (Comparative Example 2) and the addition of natural herbal extracts to the deep sea water (Comparative Examples 3 and 4) was higher, which was the highest in the Examples.

Skin side effects according to the application of the sap appeared large in Comparative Example 3 where the ratio of the natural herbal extracts were added, all the other cases were found to be insignificant. Therefore, it is preferable that the addition ratio of the natural herbal extract is 7.0 wt% or less.

division Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example Moisturizing improvement 1.05 1.26 3.98 3.38 4.85 Elasticity wrinkle improvement 1.94 2.55 3.34 3.88 4.67 Improve skin shine 1.55 2.45 3.24 3.32 4.56 Skin side effects 1.06 1.09 3.13 1.08 1.10

In the embodiment of the present invention, the highest synergistic effect is considered to have augmented the complementary effect by interacting with the active ingredients that can give each function and effect.

Therefore, the present invention is a very useful invention that can be expected to produce a complex effect of moisturizing improvement, elastic wrinkle improvement, skin shine improvement appropriate to the skin by mass production of anti-aging and antioxidant functional sap.

Claims (5)

A pretreatment step of collecting the salmon and collecting the internal organs of the salmon body, collecting and collecting the testis parts separately, washing them with alkaline electrolyzed water and disinfecting them with acidic electrolyzed water;
A drying step of immersing the pretreated salmon testis in 70-95% alcohol for 20-30 minutes and then drying at 10-20% moisture content;
Lyophilization step of pulverizing dried salmon testis and then hydrolyzing with enzyme and concentrating by freeze drying;
Salmon testis characterized in that it comprises a; stirred and prepared by mixing and stirring 0.1 to 1.0% by weight of lyophilized salmon testes, 92.0 to 94.9% by weight deep sea water and 5.0 to 7.0% by weight natural herbal extracts; Method for producing anti-aging and antioxidant functional sap for cosmetics.
The method of claim 1,
In the pretreatment process, washing with alkaline electrolytic water is cosmetic anti-aging and antioxidant functional sap using salmon testis, which is immersed in pH 10.5-11.5 strongly alkaline electrolyzed water for 2 to 5 times the weight of salmon testes. Manufacturing method.
The method of claim 1,
Sterilizing and disinfecting the acidic electrolyzed water in the pretreatment process is characterized in that the salmon testes are immersed in alkaline electrolyzed water for 5-15 minutes in 2-5 times the pH 2.5-3.5 strongly acidic electrolyzed water relative to the weight of the salmon testis. Method for producing anti-aging and antioxidant functional sap for cosmetics.
The method of claim 1,
The natural herbal extract in the stirring manufacturing process is at least one of black bean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract Method for producing anti-aging and antioxidant functional sap for cosmetics using salmon testis, characterized in that the mixture.
The method of claim 4, wherein
The black bean extract, motherwort extract, situation mushroom extract, reed root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract, bokbunja extract, red ginseng extract, each black bean, motherwort, situation mushroom, brown root , Ginseng, Centella, Pomegranate, Golden, Astragalus, Pogongyoung, Bokbunja, Red ginseng and alcoholic alcohol 95% alcohol in a ratio of 1: 5 ~ 1: 20 by weight, sealed in a sealed glass container, and then heated to 4-10 ° C. First leaching for 3-8 weeks in cool and dark place to avoid sunlight, pour out the extract, leaching and extracting each black soybean, motherwort, situation mushrooms, brown root, red ginseng, centella, pomegranate, golden, Hwanggi, Pogongyoung, Bokbunja, Red ginseng, and alcoholic beverage 25% alcohol in a ratio of 1: 5 ~ 1: 20 by weight in a sealed hermetically sealed glass container. To extract the second leaching for 3-8 weeks Weigh out the extract, leaching and extracting each of the black beans, motherwort, situation mushroom, brown root, red ginseng, Centella, pomegranate, golden, Astragalus, Pogongyoung, Bokbunja, red ginseng and pH2.5-3.5 strongly acidic electrolyzed water After mixing at a ratio of 1: 60 ~ 1: 90, black beans, motherwort, situation mushrooms, brown root, ginseng, centella, pomegranate, golden, Astragalus, pogongyoung, bokbunja, red ginseng and water at 100 ~ 120 ℃ In the ratio of 1: 20 ~ 1: 30, take 4 ~ 6 hours of boiling water and extract it in 3rd order. Extract the extract, and extract black tea, motherwort, situation mushroom, brown root, red ginseng, bottle grass, extracted 3 times with strong acid water bath. Pomegranate, Golden, Astragalus, Pogongyoung, Bokbunja, Red ginseng and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1: 60 ~ 1: 90 to weight, and then black beans, motherwort, Situation of mushrooms, brown root, red ginseng, centella, pomegranate, golden, yellow, pogongyoung, bokbunja, red ginseng and water 1: 20 ~ 1: 30 Extract the extract from the mixture by pouring it in a 4th to 6h period, and extract the extract in 4th order, and mix the extract extracted in each of the 4th phase from above, and weigh the concentrate and the extract at 10-40 ℃ by using a vacuum rotary evaporator. Concentrate for 10-40 minutes so that the ratio is 1: 10 ~ 1: 40, black bean extract, motherwort extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, golden extract, Astragalus extract, pogongyoung extract Method for producing cosmetic anti-aging and antioxidant functional sap using salmon testis, characterized in that the preparation of bokbunja extract, red ginseng extract.

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