KR20120120569A - A processing method of liquid by using deep sea water and octopus testis extract and the herbal extract for anti-bacterial - Google Patents

Abstract

PURPOSE: A method for making antibacterial and antioxidative natural fluid using octopus testis natural extract is provided to maintain skin antibacterial and antioxdative function. CONSTITUTION: A method for making antibacterial and antioxidative natural fluid using octopus testis natural extract comprises: a step of collecting a testis part and washing the testis part with alkaline electrolyzed water; a step of sterilizing and disinfecting in an acid electrolyzed water(S11); a step of dipping pretreated octopus testis I 70-95% spirit for 20-30 minutes and drying(S12); a step of pulverizing the testis and hydrolyzing with enzyme; a step of concentrating and freeze-drying(S13); and a step of mixing 0.1-1.0 wt% of octopus testis, 92.0-94.9 wt% of deep seawater, and 5.0-7.0 wt% of natural crude drug extract and stirring(S14). [Reference numerals] (S11) Pretreatment process; (S12) Drying process; (S13) Freeze-drying process; (S14) Stirring and preparing process; (S15) Sealing and packing process

Description

A method for manufacturing antibacterial antioxidant natural sap using natural extracts such as octopus testis {A PROCESSING METHOD OF LIQUID BY USING DEEP SEA WATER AND OCTOPUS TESTIS EXTRACT AND THE HERBAL EXTRACT FOR ANTI-BACTERIAL}

The present invention relates to a method for producing antimicrobial antioxidant natural sap using natural extracts such as octopus testis to prepare antimicrobial antioxidant functional sap by mixing and stirring the natural herbal extract in nucleic acid and protamine extracted from deep sea water and octopus testis. Is a method of preparing antibacterial antioxidant natural sap utilizing natural extracts such as octopus testis prepared by stirring the octopus extracted with by-products in a proper ratio of nucleic acid and protamine extracted from by-product octopus testis.

Skin is the largest organ in the human body, its size is about 1.6㎡, and it is a large organ that weighs 16% of its weight by weight. The skin serves as the most basic skin covering the body to protect internal organs. In addition, it is a very important institution that performs many functions.

The skin is constantly in contact with changes in the external environment such as ultraviolet rays, temperature changes, moisture, dust, and air pollution, and it is important to protect the individual by quickly reading the information essential for survival and allowing the body to react appropriately. In charge of the task, it has an additional function to protect the skin itself to some extent from external harm.

However, as we get older, our skin cannot deny the inherent loss of its own function, and in order to supplement it, we have to borrow the power of cosmetics following the consumption of food.

The close association between aging and oxidation is well known in many studies.

In particular, it is known that wrinkling caused by aging of the skin and blackening phenomena such as local spots and blemishes are correlated with this oxidation phenomenon. And genetic factors due to the shape of the muscles and the constant relaxation of the muscles. Furthermore, it can be divided into external factors due to environmental pollution and stress.

Skin aging caused by various factors such as keratin production, decreased fibroblast division, decreased collagen synthesis, decreased hyaluronic acid production, increased signal transduction of melanin production, wrinkles, blemishes and freckles And blemishes gradually increase.

In order to improve such skin aging, AHA, retinoic acid, retinol, ascorbic acid and the like have been mainly used to apply the herbal extracts containing the antioxidant polyphenols.

Research has shown that the major components of fish testes are nucleic acids, proteins, and most protamines.

Lipid peroxide suppression and protamine have been reported to have antimicrobial and electron donating ability against Gram-positive bacteria.

Nucleic acid and protamine, which are widely distributed in octopus testis, are essential proteins for our body and are important components of antioxidant enzymes that protect cells from free radicals generated during normal oxygen metabolism.

This can be said to be an essential ingredient for the normal functioning of the immune system. Therefore, if it is extracted, purified and emulsified in the testis, which is a by-product of octopus, and can be absorbed through the skin, this will continue to be used in the cosmetic industry. The research could be more active.

In addition, researches and product launches of natural cosmetics, which are made by mixing natural herbal extracts and antibacterial functional additives to help wrinkle improvement, skin elasticity and whitening, are actively underway.

Currently, functional cosmetics in Korea are categorized into three categories: Wrinkle improvement products, whitening products, and UV protection products recognized by the Korea Food and Drug Administration through the review of functional cosmetics. As a product for improving functional anti-wrinkle with the addition of an antioxidant functional composition, it is being developed and marketed and relatively expensive products are the mainstream.

Therefore, the present invention relates to a method for producing a sap that can give antimicrobial antioxidant functionality using natural herbal extracts to nucleic acids and protamine extracted from deep sea water and octopus testis, skin aging, lack of elasticity and whitening with age As the sap that can meet the needs of the general consumer who needs antibacterial and moisturizing skin as well as the elderly age to be replenished, the purpose is to enable mass production and distribution sales.

In order to solve the object of the functional improvement of the skin as described above, the method for producing an antibacterial antioxidant functional sap of the present invention is to occupy the octopus to collect the internal organs of the octopus, separate and collect the testis part and wash in alkaline electrolytic water and acid A pretreatment step of disinfecting the electrolyzed water; A drying step of immersing the pretreated octopus testine in 70-95% alcohol for 20-30 minutes and then drying at 10-20% moisture content; A freeze-drying process of grinding dry octopus testis, hydrolyzing using an enzyme, concentrating and freeze-drying; 0.1-1.0 wt% of lyophilized octopus testis, 92.0-94.9 wt% of deep seawater, and 5.0-7.0 wt% of natural herbal extracts are mixed and agitated to prepare a sap.

In addition, the washing process in the alkaline electrolytic water of the pretreatment step in the method for producing an antibacterial antioxidant functional sap of the present invention is characterized in that it is immersed in a pH 10.5-11.5 strong alkaline electrolytic water of 2-5 times the weight of the octopus test weight 5-15 minutes. do.

 In addition, sterilization and disinfection in the acidic electrolyzed water of the pretreatment step in the method for producing an antibacterial antioxidant functional sap of the present invention in a pH 2.5-3.5 strongly acidic electrolyzed water of 2-5 times the weight of the octopus internal organs washed in alkaline electrolyzed water It is characterized by immersion for 5-15 minutes.

In addition, the natural herbal extract of the stirring step in the manufacturing method of the antibacterial antioxidant functional sap of the present invention is black sesame extract, lily extract, situation mushroom extract, brown root extract, red ginseng extract, centella extract, pomegranate extract, Angelica extract, Astragalus extract, It is characterized by consisting of pogongyoung extract, wolfberry extract, ginseng extract.

 In addition, in the method for producing an antibacterial antioxidant functional sap of the present invention, the black sesame extract is mixed with a black sesame and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 in weight ratio of 4-10 degrees C. Let the primary leaching extract for 3-8 weeks in a cool dark place to avoid temperature and sunlight, pour out the extract, leaching the black sesame and alcohol content 25% alcohol at a ratio of 1: 5 to 1:20 by weight. After sealing in a sealed hermetically sealed glass container, the second leaching and extraction were carried out for 3-8 weeks in a cold dark place where temperature and 4-10 degrees Celsius could be avoided. 3.5 After mixing strongly acidic electrolyzed water at a ratio of 1:60 to 1:90, and extracting it in a third time by boiling water for 4-6 hours at a temperature of 100 to 120 ° C. so that the ratio of black sesame and water is 1:20 to 1:30. Pour out the extract and pour it into the strong acid water bath The extracted black sesame and pH10.5-11.5 strongly alkaline electrolyzed water were mixed at a ratio of 1:60 to 1:90, and the ratio of the black sesame and water was 1:20 to 1:30 at a temperature of 100 to 120 ° C. Extract the extract from each of the four extracts above, extract the extract from the 4th mixture by boiling water for 6 hours, and use the vacuum rotary evaporator to mix the concentrate and the extract with a weight of 1 to 40 ° C. Characterized in that the manufacturing for 10-40 minutes to the ratio of 10 ~ 1:40,

The lily extract is sealed in a sealed glass container mixed with a lily and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then 3-8 in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius. The primary leaching and extraction were carried out, followed by the extract, and the leached and extracted primary liquor and alcoholic alcohol 25% spirits were sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 to the weight of 4-10 degrees C. To extract the second leaching for 3-8 weeks in a cool dark place that can avoid the temperature and sunlight of the solution, pour out the extract, leaching the second extraction lily and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ 1: 90 After mixing in the ratio of 100 ~ 120 ℃ the lily and water in a ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours in a water bath to extract the third extract, poured into a strong acid water bath 3 The extracted lily and the pH10.5-11.5 strongly alkaline electrolyzed water in a ratio of 1:60 to 1:90 by weight After mixing to 100 ~ 120 ℃ at a temperature of 100 ~ 120 ℃ the ratio of 1:20 ~ 1:30 in a 4 ~ 6 hours in a water bath to extract the fourth step to extract the extract, from above each of the fourth Mixing the extract and using a vacuum rotary evaporator is characterized in that for 10 to 10 minutes to prepare a concentration of the concentrate and the extract in a ratio of 1:10 to 1:40 by weight in a temperature setting condition of 10-40 ℃, characterized in that

The situation mushroom extract is sealed in a sealed glass container mixed with a 95% alcohol content of the situation mushrooms and alcohol in a ratio of 1: 5 to 1:20, and then in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius After the first leaching extraction for 3-8 weeks, pour out the extract, and then rinse the firstly extracted situation mushroom and alcoholic beverage 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed glass container. Compared to the weight of the situation mushroom and pH2.5-3.5 strongly acidic electrolyzed water extracted by distilling and extracting the secondary extract for 2 to 8 weeks in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius. After mixing at a ratio of 1:60 to 1:90, the situation mushroom and water were heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. for 4 to 6 hours, and extracted in a third manner. Sichuan mushroom and pH10.5-11.5 strongly alkaline electrolyzed water After mixing at a ratio of 1:60 to 1:90 to the weight of 100% to 120 ℃ temperature of the mushrooms and water so that the ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours to be extracted in the fourth Extract the extract, mix the extract extracted from each of the above 4 times, and use the vacuum rotary evaporator to make the concentrate and the extract at a ratio of 1:10 to 1:40 by weight at a temperature of 10-40 degrees Celsius. It is characterized in that to prepare for 10-40 minutes to concentrate, the extract of the root is 4-10 degrees after sealing the sealed airtight glass container with the ratio of 1: 5 ~ 1:20 weight ratio of the weight of the root and alcohol 95% Let the primary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the seed, pour out the extract, leaching and extracting the first extract of the root and alcohol content 25% 1: 5 ~ 1: 20 Sealed in a sealed glass container at a ratio of 4-10 degrees Celsius and avoid sunlight After the second leaching and extraction in the cool and dark place for 3-8 weeks, the extract was poured out, and the leached and extracted secondary roots and the pH2.5-3.5 strongly acidic electrolyzed water were mixed at a ratio of 1:60 to 1:90 by weight. At the temperature of 100-120 ° C., the roots and the water are boiled for 3 to 4 hours in a ratio of 1:20 to 1:30, followed by extraction in the third step, followed by the extract. 5-11.5 After mixing strongly alkaline electrolyzed water at a ratio of 1:60 to 1:90, the root and water in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. for 4 to 6 hours To extract the extract 4 times, followed by extracting the extract from each of the above 4 times, and using the vacuum rotary evaporator at a temperature of 10-40 degrees Celsius setting the concentrated solution and the extract to the weight 1:10 ~ 1 It is characterized in that it is prepared by concentrating for 10-40 minutes to a ratio of 40,

The ginseng extract is sealed in a sealed glass container mixed with ginseng and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. After 8 weeks of primary leaching extraction, the extract was drained, and then the leached and extracted primary ginseng and alcoholic alcohol 25% spirits were sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunshine of the seed, pour out the extract, leaching the extracted second ginseng and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the ginseng and water are heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C for 4 to 6 hours to be extracted in a third manner, followed by extracting the extract, and a strong acidic water. Ratio of 1: 60 ~ 1: 90 to the weight of Gosam and tertiary extracted Alkaline ginseng and pH10.5-11.5 strongly alkaline electrolyzed water After mixing to 100 ~ 120 ℃ the ginseng and water in a ratio of 1:20 ~ 1:30 in 4 to 6 hours in a water bath so that the extract is extracted 4 times, from above each of the fourth round The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,

The Centella asiatica extract is sealed in a hermetically sealed glass container in a ratio of 1: 5 to 1:20 by weight ratio of 95% alcohol and alcohol in a bottle, and then cooled in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained, and the leaching and extracting the primary grass and alcoholic beverage 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 Leak extraction for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching second extraction centrifugal grass and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the centella and the water at a temperature of 100 ~ 120 ℃ 4 to 6 hours so that the ratio of 1:20 ~ 1:30 so that it is extracted in the third step to extract the extract, strong acidic water Ratio of 1: 60 ~ 1: 90 to the weight of the centrifugal grass extracted from the bath and the pH10.5-11.5 strong alkaline electrolyzed water After mixing at 100 ~ 120 ℃ temperature of the centrifugal grass and water in a ratio of 1:20 ~ 1:30 in 4 to 6 hours to be extracted in 4th to extract the extract, from above each 4 times The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,

The pomegranate extract is sealed in a sealed glass container mixed with pomegranate and alcoholic alcohol 95% alcohol in a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place to avoid temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained, and the leached and extracted primary pomegranate and alcoholic alcohol 25% spirits are sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the second extraction pomegranate and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing in a ratio of 1:90, the pomegranate and water are heated at 100-120 ° C. for 4-6 hours in a ratio of 1: 20-1: 30, followed by extraction in 3rd order, followed by the extract, and strongly acidic water. The ratio of pomegranate extracted from the water bath and the pH10.5-11.5 strong alkaline electrolyzed water in the ratio of 1:60 to 1:90 After mixing to 100 ~ 120 ℃ temperature of the pomegranate and water in a ratio of 1:20 ~ 1:30 in 4 to 6 hours to be extracted in 4th to extract the extract, from above each of the fourth The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,

The Angelica extract is sealed in an airtight glass container mixed in the ratio of 1: 5 to 1:20 weight ratio of alcohol and alcohol in the ratio of 1: 5 to 1:20, and then 3-8 in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius 1 weekly leach extraction, drain the extract, and then leaching and extracting the primary extract and alcoholic alcohol 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 degrees To extract the second leaching for 3-8 weeks in a cool dark place that can avoid the temperature and sunlight, pour out the extract, leaching the extracted Angelica and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ 1: 90 After mixing in the ratio of 100 ~ 120 ℃ temperature and the water in the ratio of 1:20 ~ 1:30 in the ratio of 1: 20 ~ 1: 30 in a water bath to extract the third to pour the extract, the strong acid water water bath 3 Extracted Angelica and pH10.5-11.5 strongly alkaline electrolyzed water at a ratio of 1:60 to 1:90 After mixing to 100 ~ 120 ℃ in the temperature of 100 ~ 120 ℃ the ratio of 1:20 ~ 1:30 in a 4 ~ 6 hours in a water bath to extract the fourth step to extract the extract, from above each of the fourth Mixing the extract and using a vacuum rotary evaporator is characterized in that for 10 to 10 minutes to prepare a concentration of the concentrate and the extract in a ratio of 1:10 to 1:40 by weight in a temperature setting condition of 10-40 ℃, characterized in that

The Astragalus extract is sealed in an airtight glass container mixed with a ratio of 1: 5 to 1:20 of Astragalus and alcoholic alcohol at 95% alcohol by weight, and then cooled in a cool dark place that can avoid temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained and the leaching and primary extraction of sulfur and alcoholic alcohol 25% alcohol is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the secondary extract and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ After mixing at a ratio of 1:90, the sulfuric acid and water are heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. for 4 to 6 hours to be extracted in a third manner, followed by extracting the extract, and a strong acidic water. Ratio of 1:60 to 1:90 ratio of the weight of sulfuric acid and pH10.5-11.5 strong alkaline electrolyzed water After mixing to 100 to 120 ℃ the sulfur and water in a ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours in a hot water to extract the fourth step, the extract is poured over each of the fourth The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,

The pogongyoung extract is sealed in a closed glass container mixed with poongongyoung and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. Let the primary leaching extract for 8 weeks, drain the extract, seal the pogongyoung and alcohol distilled liquor 25% alcohol in the ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the pogongyoung and pH2.5-3.5 strongly acidic electrolyzed water extracted from 1: 60 ~ After mixing at a ratio of 1:90, the pogongyoung and water at a temperature of 100 ~ 120 ℃ 4 to 6 hours so that the ratio of 1:20 ~ 1:30 to be extracted in a third to extract the extract, strong acidic water Pogongyoung and pH10.5-11.5 strongly alkaline electrolyzed water The mixture is mixed at a ratio of 1:60 to 1:90, followed by 4 to 6 hours of boiling water at a temperature of 100 to 120 ° C. so that the poongyoung and water are at a ratio of 1:20 to 1:30. The extracts were extracted from each of the above four rounds, and the concentrates and extracts were mixed at a temperature of 10-40 degrees Celsius using a vacuum rotary evaporator at a ratio of 1:10 to 1:40 by weight. Characterized in that the concentration is prepared for a minute,

The wolfberry extract is sealed in a sealed glass container mixed with a wolfberry and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then in a cold dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius 3- After 8 weeks of primary leaching extraction, the extract is poured out, and the leached primary extract and alcoholic beverage 25% spirits are sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. The leaching and extraction of the extract from the cool and dark place to avoid the temperature and sunshine of 3 degrees for 8 weeks, drained the extract, leaching the second extraction Gojija and pH2.5-3.5 strongly acidic electrolyzed water 1:60 ~ After mixing at a ratio of 1:90, the Goji and water at a temperature of 100 ~ 120 ℃ 4 to 6 hours so that the ratio of 1:20 ~ 1:30 to be extracted in a third to extract the extract, strong acidic water Comparison of Goji Goji Extracted with Water Bath and pH10.5-11.5 Strongly Alkaline Electrolyzed Water After mixing at a ratio of 1:60 to 1:90, the mixture of the Goji berries and water at a temperature of 100 to 120 ° C. for 4 to 6 hours in a ratio of 1:20 to 1:30 is extracted for the fourth time, and the extract is extracted. The extracts were extracted from each of the above four rounds, and the concentrates and extracts were mixed at a temperature of 10-40 degrees Celsius using a vacuum rotary evaporator at a ratio of 1:10 to 1:40 by weight. Characterized in that the concentration is prepared for a minute,

The ginseng extract is sealed in a sealed glass container mixed with ginseng and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained, and the leaching and primary extraction of ginseng and alcohol 25% alcohol is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the seed, rinse the extract, leaching the extracted second ginseng and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the ginseng and water are heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C for 4 to 6 hours, followed by extraction in 3rd order, followed by extracting the extract, and a strong acidic water. The ratio of ginseng extracted from the water bath and the pH10.5-11.5 strongly alkaline electrolyzed water to the weight ratio of 1:60 to 1:90 After mixing to 100 ~ 120 ℃ the ginseng and water at a temperature of 1:20 ~ 1:30 in a ratio of 4 to 6 hours to be extracted in 4th to extract the extract, and from above each 4 times The extracted extract is mixed and prepared by concentrating the extract and the extract for 10-40 minutes using a vacuum rotary evaporator at a temperature of 10-40 ° C. in a ratio of 1:10 to 1:40 by weight. .

 In the pretreatment step of the present invention, the octopus used is not limited to the type and size of the octopus, such as domestic coastal mountains, overseas Pacific mountains, Atlantic mountains, Indian Ocean production.

In the concentration step of the present invention, the water temperature in the extraction concentration tank used during the enzymatic hydrolysis is characterized in that the preheating operation for 0.5-5.0 hours while maintaining 25-50 ° C.

In the sealing packaging step of the present invention, weighing the sap prepared by stirring and weighing the contents and weighed and sealed with a molding container made of PP, PE, PS, glass, the product packaged through a metal detector and then circulated after delivery It is done.

According to the method of preparing mixed antimicrobial antioxidant functional sap by mixing and stirring nucleic acid extracted from deep seawater and octopus testes of the present invention according to the above-described composition, protamine and natural herbal extracts, there is a great difficulty in supplying raw materials of testes which are octopus by-products in the pretreatment step. It is effective in improving understanding of antibacterial antioxidant skin care that has been neglected by Korean people and by easily absorbing functional fluids, so that anyone can understand and immediately check for proper skin antibacterial antioxidant recovery, maintenance and adaptation. To provide.

1 is a flow chart showing a manufacturing process of the antibacterial antioxidant functional sap for antibacterial cosmetics according to an embodiment of the present invention.

The technical problem achieved by the present invention and the practice of the present invention will be more clearly understood by preferred embodiments of the present invention described below.

The following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings and experimental examples.

1 is a flow chart illustrating a method for preparing antimicrobial antioxidant functional sap by mixing and stirring a natural herbal extract to nucleic acid and protamine extracted from deep sea water and octopus testis according to an embodiment of the present invention.

Referring to the drawings, the pre-treatment step of collecting the octopus by collecting the octopus to collect the internal organs of the octopus, and separate and collect the testis part, wash in alkaline electrolytic water and sterilize in acidic electrolytic water (S11); Drying step (S12) of immersing the pretreated octopus testine in 70-95% alcohol for 20-30 minutes and drying it at a moisture content of 10-20%; Lyophilization step of crushing the dried octopus testis, hydrolyzing with enzyme and concentrating and freeze drying (S13); A stirring manufacturing step (S14) of preparing a sap by mixing and stirring 0.1-1.0 wt% of lyophilized octopus, 92.0-94.9 wt% of deep seawater, and 5.0-7.0 wt% of the natural herb extract; Sealing packaging step (S15) to put the prepared sap in a container to seal the packaging; consists of.

In particular, a method for preparing an sap that can give antimicrobial antioxidant functionality using nucleic acid and protamine extracted from deep seawater and octopus testis in accordance with an embodiment of the present invention, predominantly alkaline electrolyzed water in octopus test during pretreatment It is immersed in the solution for 5-15 minutes, washed and washed. Secondly, it is sterilized by immersing it in acidic electrolytic water immersion solution for 5-15 minutes. Therefore, electrolyzed water is needed to prepare the immersion liquid. The strong acid electrolytic water and the strong alkaline electrolyzed water are collected, and all of the collected strong electrolytic water can be effectively utilized by preparing an immersion liquid for each purpose.

Looking at this in more detail, the above-described pretreatment step (S11) is a process of collecting tap water drinking water, which is a raw material, and electrolyzing it in an electrolytic cell to produce and use the produced alkaline electrolytic water and acid electrolytic water as an immersion aqueous solution, and also impossibility of octopus. It is a process of removing and washing the intestinal filth and foreign substances, which are the food parts, and collecting them.

When the water temperature of the electrolyte is increased during the generation of electrolytic water, the ionic change is activated in the diaphragm in the electrolytic cell, so that the strong acid electrolytic water generated at the anode not only increases the concentration of hypochlorite (HOCL), which is an effective chlorine having a disinfectant disinfection effect, but also the steel produced at the cathode. Alkaline electrolyzed water has a high concentration of sodium hydroxide (NaOH) having an interfacial effect, so that an underwater heater, which is a heating facility, is installed in a distilled water reservoir to operate at a temperature of 35-40 degrees Celsius.

In addition, in the above-described electrolysis process, 0.1-10.0% sodium chloride (NACL) is added to distilled water, mixed and diluted to make an electrolyte, and then electrolyzed in a electrolyte membrane electrolyte tank in an electrolytic cell. Exhaust pipes are installed to allow the discharge of chlorine gas from the anode.

In general, electro-analysised water refers to water obtained by applying electric force to general water such as tap water or ground water.

If the anode and cathode platinum-plated titanium is put into the water and the electricity is supplied by direct current, the anion dissolved in the water is collected on the (+) pole to become acidic ionized water, and cations gather on the (-) pole to the alkaline ionized water. Occurs.

The electrolyzed water thus produced has its ionic effect for 7-8 days when exposed to air and about 3-4 weeks when stored with the lid closed.

In the process of collecting the strongly alkaline electrolyzed water and the strongly acidic electrolyzed water generated in the above-mentioned electrolytic water collection process for use as the strongly alkaline pretreatment aqueous solution and the strongly acidic pretreatment aqueous solution, the strongly acidic electrolytic water at the anode side and the strong side at the cathode side were produced. Collect alkaline electrolytic water separately and put it in the sump and circulate so that the water temperature is maintained at 5-10 degrees Celsius using chiller, a cooling facility in each sump.

Underwater UV sterilizer is installed and operated in each reservoir to sterilize so that pH property does not change.

In addition, care should be taken to prevent re-contamination of each collected ionized water.

In case of acidic ionized water, it has a converging effect and constricts the skin.

Therefore, since it protects the skin slightly acidic, it is of course excellent in skin care and disinfection.

Hypochlorite Water Additive Standard by Korea Food and Drug Administration and Hypochlorite Water Food Additive Approval Standard by Japan Ministry of Health and Welfare applies the same standards and standards, according to which the component standard of Hypochlorous Acid Water is strong acidic hot water. (Also called strongly acidic hypochlorous acid) is less than pH2.7 and contains 20-60ppm of hypochlorous acid (HOCL), an effective chlorine, and non-acidic water (also called non-acidic hypochlorous acid) is pH5.0-6.5 It contains 10-30ppm of hypochlorous acid (HOCL), and 10ppm of effective chlorine has a bactericidal effect against bacteria with low disinfectant resistance, and 20-30ppm of effective chlorine has a bactericidal effect against bacteria that do not have apopoies. An effective chlorine of 60ppm has a bactericidal effect within minutes of infant bacilli.

Sodium hypochlorite water is not effective in killing follicles, but strong acid hypochlorite is a very effective fungicide for follicles, and Bacillus and clostridium have a killing effect within 3 minutes.

Hypochlorite water is characterized by a strong bactericidal and deodorizing effect but very low biotoxicity. The bactericidal power is fungus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and other nutrients, Cereus, Acidophilus, Fungi, Viruses, Heterogeneous bacteria, Salmonella It has strong bactericidal power against food poisoning such as bacteria and Vibrio bacteria, as well as all pathogenic microorganisms caused by raw water. (Source: less than 5 seconds of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and other trophic bacteria of less than 5 minutes , 1 min-2 min 30 sec, fungus 5 sec-1 min, virus less than 5 sec, antimicrobial effect of strong acidic electrolyzed water, clinical test, 37: 919-919, 1993) Salmonella and Vibrio bacteria were not detected after 120,000-200,000 bacteria at 30 seconds after treatment, Test report on sterilization ability, Korea Testing and Research Institute, 2002) Hypochlorite is a fundamental measure of sterilization The odor is weak and experimentally 80 times stronger than sodium hypochlorite (NAOCL) and practically 5-10 times stronger, so the effect is that the bactericidal power of hypochlorite water with 30 ppm chlorine concentration is hypochlorous acid with 150 ppm chlorine concentration The US FDA (Food and Drug Administration) recognizes 1/50 of sodium hypochlorite in terms of the biotoxicity of hypochlorite water as well as being superior to sodium water (a disinfectant diluted in water). It is already widely used as a substitute for chemicals such as food disinfectants and pesticides.

Hypochlorite water is already used as an optimal disinfectant for airborne poisoning due to water, food raw materials, and spray because it is free of odors and does not contain salts.

Sterilization is increased by increasing the temperature of hypochlorous acid.

Strong alkaline ionized water is generated on the opposite (-) side as much as the amount of strong acidic water is made on the hypochlorite water production device (+) side, and serves as a surfactant, so strong alkaline ionized water is used as a cleaning agent, chemicals such as wax, chemical fertilizer, etc. It is also used as a substitute.

In addition, alkaline ionized water has more beneficial minerals (inorganic salts) than normal water, and makes water molecules smaller, so the absorption of minerals is increased, which helps improve diarrhea and constipation, and works on the stomach.

Strong alkaline ionized water is a natural emulsifier with excellent dissolving and detergency, as well as a surfactant with excellent natural surfactant effect, containing 40-120 ppm of sodium hydroxide (NaOH) at pH11.0 or higher. Because of its physical properties, it has a function to improve cleaning power, so it is mainly used for cleaning raw materials such as root vegetables (potatoes, potatoes, carrots, etc.), fruit vegetables (cucumbers, pumpkins, tomatoes, etc.), and grains (beans, brown rice, etc.). .

In foreign countries, strong alkaline water with a pH greater than 11.0 is recognized as a dilution solution of sodium hydroxide solution. It contains sodium hydroxide and natural emulsifiers and functions as a non-toxic natural detergent. It is due to the effect of washing off grease well.

The cleaning power is increased by increasing the temperature of the strong alkaline water.

In the case of countries where electrolyzed water is legalized by strong acid and strong alkaline ionized water, Kangsan Electrolyzed Water was announced in Korea in December 2005 by the Korea Food & Drug Administration (Packaging Container Team) No. 2005-75. It was first used as a standard and standard for hypochlorite, which is a disinfectant for facilities, and was announced as No. 2007-74 of the Korea Food and Drug Administration (Food Additives Team) in November 2007. And specifications.

Foreign cases preceded us. In June 2002, the Ministry of Health, Labor and Welfare Ordinance No. 75 and Notice No. 212 recognized strong acidic water as hypochlorous acid and approved as a food additive (sterilizer). In 1999, the sanitary water generator was approved by the Ministry of Sanitation as a disinfection device, and in 1998, the EPA (Environmental Protection Agency) approved the strong electric water generation device as a disinfectant manufacturing device in 1999, and in 1999, the FDA approved the strong acid electrolytic water. Recognized as the same as hypochlorous acid water, it was applied as a disinfectant for food materials and utensil containers, and the same year USDA (Agricultural) approved the use of electrolytic hypochlorous acid for the purpose of disinfecting intestinal pathogens of growth.

In foreign countries, since 1996, in Japan, hand pesticides, endoscope disinfection, antibacterial measures, prevention of infection in the hospital, treatment of burns, oral disinfection, and pesticide-free pesticides for crop disease control in agriculture since 1996. In 2002, it was used for cultivation and maintaining freshness of harvest.In 2002, it was used for the removal of organic odors in the environment, decomposition of pollutants, and the production of drinking water. In addition, its use has been extended to improve and preserve the quality by strong alkaline electrolyzed water.

Therefore, strong acidic electrolyzed water is a product that is approved for use in food hygiene not only in Korea but also in the world. It is harmless to humans, has no residue, and has excellent sterilizing power as can be seen from the above examples. .

As described above, the characteristics of each strong electric seawater are summarized first, and then the strong alkaline electrolyzed water should be cleaned and then sterilized and disinfected with strong acidic hypochlorite water as a rule. If the object is first immersed in strong alkaline electrolyzed water to remove organic contaminants and washed and disinfected with strong acidic electrolyzed water, the sterilization effect can be expected to be greatly increased.

Therefore, in the present invention, first, by setting the order to sterilize sterilization by immersion in strong alkaline electrolyzed water, immersed in strong acid electrolytic water, sterilization and disinfection to maximize the effect.

Looking at this in more detail, the above-described pretreatment step (S11) is a process of circumscribing the octopus to immerse the by-product octopus internal organs in distillation and alkaline electrolyzed water to clean and wash and sterilize by sterilization in acid electrolytic water.

Generally, raw materials can use all kinds of octopus, such as domestic coastal, Pacific, Indian, and Atlantic.

In addition, octopus is a dried or specially processed biological octopus can be used both frozen and living octopus, if frozen, thawed.

In the case of thawing frozen octopus, thaw without inundating in a refrigerated thawing room with an outdoor temperature of minus 5 ° C or above, and use a fan for quick thawing.

The octopus consists of the head, the body and the invisible parts of the eyes, bones, teeth, and intestines.The octopus collects the intestines, and separates and separates the testes. Washing water and dirt in flowing water, and prepared pH10.5-11.5 alkaline electrolyzed water and pH2.5-3.5 acidic electrolyzed water with immersion solution 2-5 times the weight of testes, and collecting them in an electrolytic water tank. Immerse in electrolytic water immersion tank for 5-15 minutes, and secondly immerse in acid electrolytic water immersion tank for 5-15 minutes.

In this case, for hygiene, work table, knife, cutting board, etc. should be disinfected frequently using 100 ~ 200ppm solution of chlorine or 20 ~ 25ppm solution of iodine.

The drying step (S12) described above is a process of first drying the pretreated octopus testis at a moisture content of 10-20%.

The above-mentioned stirring preparation step (S14) is the enzyme-hydrolyzed and concentrated lyophilized high nucleic acid, protamine, deep sea water and natural herbal extract in a weight ratio of 0.1-1.0: 92.0-94.9; Mix at a rate of 5.0-7.0 and finally stir with a homogenizer for 30-45 minutes at a temperature condition of 50-70 degrees Celsius.

In the above-described configuration, the sealing packaging step (S15) is weighed and prepared by weighing the sap prepared by stirring the contents and sealed in a container to pass through the metal detector through the packaged product immediately after distribution.

Each of the sap prepared above is taken to measure pH.

In this case, the container should be sterilized by air drying after immersion in the disinfectant solution using alcohol, strong acidic electrolyzed water and iodine.

In the above series of processes, in order to prevent the growth of microorganisms at room temperature, the elapsed time of the process should be made within 4 hours during the stirring packaging process and the sealing packaging and the rapid freezing process.

Hereinafter, a method for preparing antimicrobial antibacterial antioxidant functional sap using deep seawater and octopus extracted nucleic acid, protamine and natural herbal medicine prepared according to an embodiment of the present invention, and sap prepared according to the comparative example and other sap prepared according to the comparative example Compare the results of experiments with various characteristics.

Hereinafter, a method for preparing antimicrobial antibacterial antioxidant functional sap using deep seawater and octopus extract nucleic acid, protamine and natural herbal medicines according to an embodiment of the present invention, and a sap manufacturing process prepared according to the same will proceed as follows.

[Pretreatment Process]

1) Collect octopus, a byproduct of octopus, which is removed by halting the octopus on the workbench, and remove the foreign substance after sorting and washing it under running water, weighing 1,000g, immersing in alkaline electrolyzed water for 10 minutes to wash, wash and acidic electrolyzed water. Sterilize by soaking for a minute.

2) In the electrolysis process, weigh 20.0 g of tap water and 30.0 g of sodium chloride relative to the weight of distilled water in an electrolytic solution, stir it in an electrolytic cell, and then electrolyze it in a diaphragm electrolytic cell in the electrolytic cell.

An underwater heater, which is a heating facility, is installed in the alkaline electrolytic water collector generated by the cathode to operate the water temperature at 35 degrees Celsius.

10,015g strongly alkaline electrolyzed water and 10,015g strongly acidic electrolyzed water generated in the above-mentioned electrolytic water collection process are collected for use as a strong alkaline pretreatment aqueous solution and a strong acid pretreatment aqueous solution, respectively. The strong alkaline electrolytic water on the cathode side is collected separately and placed in water collecting tanks, and the water temperature is maintained at 5-10 degrees Celsius by using a chiller as a cooling facility in each water collecting tank.

Underwater UV sterilizer is installed and operated in each reservoir to sterilize so that pH property does not change.

In addition, care should be taken to prevent re-contamination of each collected ionized water.

[Drying process]

3) The pretreated octopus testes are dipped in 70-95% alcohol for 20-30 minutes and dried at 10-20% moisture content.

Even in this case, the food contact surfaces around the cold and warm air drying chambers or work benches are frequently disinfected using 70% alcohol solution.

[Freeze drying step]

4) Grind dry octopus testis, hydrolyze using enzyme, concentrate and freeze-dry.

[Stirring step]

5) Prepare sap for improving antimicrobial antioxidant by adding moisturizer and functional additives to nucleic acid and protamine extracted from hydrolyzed, concentrated and freeze-dried pure octopus testis.

0.5g nucleic acid and protamine, 93.5g desalted deep sea water, 0.5g black sesame extract, 0.5g lily extract, 0.5g situation mushroom extract, 0.5g brown root extract, 0.5g red ginseng extract, 0.5g centella Extract, 0.5g pomegranate extract, 0.5g Angelica extract, 0.5g Astragalus extract, 0.5g Pogongyoung extract, 0.5g Wolfberry extract, 0.5g ginseng extract at a temperature of 70 degrees C. Final stirring

[Sealing packing step]

7) Each of the sap prepared above is sorted, put in a PP container, sealed, sealed packaging, and passed through a metal detector.

8) Store the product at room temperature and distribute it at room temperature.

Hereinafter, a method of preparing a sap that can give antimicrobial antioxidant functionality using natural herbal extracts from nucleic acids and protamine extracted from concentric layered water and octopus by-products prepared according to an embodiment of the present invention and the sap prepared according to the present invention Compare the results of experiments with various properties of the sap prepared according to the example.

[Experimental Example 1] sensory test on the improvement of antibacterial antioxidant wrinkles on sap that can give antimicrobial antioxidant functionality using natural herbal extracts to nucleic acid and protamine extracted from conscience and octopus testis prepared in the above process It was.

Experimental Example 1

0.5% by weight of nucleic acid, protamine and 99.5% by weight of purified water (Comparative Example 1), 0.5% by weight of nucleic acid, protamine and 99.5% by weight of deep sea water (Comparative Example 2), 0.5% by weight Of nucleic acid, protamine, and 90.5% by weight of deep seawater and 9.0% by weight of natural herbal extract (the same composition as in the present invention) (Comparative Example 3), 0.5% by weight of nucleic acid and protamine and 97.5% Each of the sap (Example) prepared according to the embodiment of the present invention is mixed with a deep sea water of 2.0% by weight natural herbal extract (the same configuration as the embodiment of the present invention) (comparative example 4) To evaluate the antimicrobial antioxidant effect of two weeks after application to the skin, a comprehensive sensory evaluayion of moisturizing, elastic wrinkles, skin shine and skin side effects was performed.

The sensory inspectors were 20 males and females who were trained in their 20s and 40s.The 5-point scoring method (5: very strong, 4: strong, 3: normal, 2: weak, 1: very weak) was used. The sample was repeated and averaged, and the sample was applied twice a day by lightly spreading on the skin under the condition of application after washing morning and evening twice a day for two weeks. Moisturizing improvement, elastic wrinkle improvement, skin lubrication improvement, skin side effects were evaluated and arithmetic average (Table 1).

As a result of the experiment, the moisturizing improvement, the elastic wrinkle improvement, the skin luster improvement, and the skin side effect effect to find the antimicrobial antioxidant improvement effect were higher in the case of the deep sea water than the case of using the water base as the general purified water (Comparative Example 1) ( Comparative Example 2) The addition of natural herbal extracts to the deep sea water (Comparative Examples 3 and 4) was higher and was the highest in the Examples.

Skin side effects according to the application of the sap appeared large in Comparative Example 3 where the ratio of the natural herbal extracts were added, all the other cases were found to be insignificant. Therefore, it is preferable that the addition ratio of the natural herbal extract is 7.0 wt% or less.

(Table 1)

In the embodiment of the present invention, the highest synergistic effect is considered to have augmented the complementary effect by interacting with the active ingredient that can give each function and effect.

Therefore, when mass-producing cosmetic sap by the method for producing an antibacterial antioxidant functional sap of the present invention, it is possible to expect a complex effect of moisturizing improvement, elastic wrinkle improvement, and skin shine improvement appropriate to the skin. It is a useful invention that can be provided.

Claims (5)

A pretreatment step of collecting octopus by collecting octopus and collecting the internal organs of the octopus, separating and collecting testis parts, washing with alkaline electrolyzed water and disinfecting with acid electrolyzed water;
A drying step of immersing the pretreated octopus testine in 70-95% alcohol for 20-30 minutes and then drying at 10-20% moisture content;
A freeze-drying process of grinding dry octopus testis, hydrolyzing using an enzyme, concentrating and freeze-drying;
A stirred manufacturing process of mixing and stirring 0.1-1.0 wt% of lyophilized octopus testes, 92.0-94.9 wt% of deep seawater, and 5.0-7.0 wt% of natural herbal extracts;
Method for producing antibacterial antioxidant natural sap utilizing natural extracts, such as octopus testis, characterized in that comprises a.
The method of claim 1,
In the pretreatment step, the process of washing in alkaline electrolytic water is an antibacterial antioxidant natural sap utilizing natural extracts such as octopus testis, which is immersed in pH 10.5-11.5 strongly alkaline electrolyzed water for 2 to 5 times the weight of octopus test weight. Manufacturing method.
The method of claim 1,
Sterilizing and disinfecting the acidic electrolyzed water in the pretreatment step includes octopus testes, which are immersed in alkaline electrolyzed water for 5-15 minutes in 2-5 times the pH 2.5-3.5 strongly acidic electrolyzed water relative to the weight of the octopus. Method for producing antibacterial antioxidant natural sap using extract.
4. The method according to any one of claims 1 to 3,
In the stirring manufacturing step, the natural herbal extracts are composed of black sesame extract, lily extract, situation mushroom extract, root extract, red ginseng extract, centella extract, pomegranate extract, Angelica extract, Astragalus extract, pogongyoung extract, wolfberry extract, ginseng extract Method for producing antibacterial antioxidant natural sap utilizing natural extracts such as octopus testis.
5. The method of claim 4,
The black sesame extract is sealed in a closed glass container mixed with black sesame seeds and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20, and then 3-8 in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius 1 weekly leach extraction, drain the extract, and then leached black sesame seed and alcohol content 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 degrees In the dark place to avoid the temperature and sunlight, the second leaching extraction for 3-8 weeks to drain the extract, leaching the second extraction black sesame and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ 1: 90 After mixing at a rate of 100 ~ 120 ℃ the black sesame and water in a ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours to be extracted in a third step to pour the extract, the strong acid water bath 3 Comparison of Extracted Black Sesame Seeds with pH10.5-11.5 Strongly Alkaline Electrolyzed Water After mixing at a ratio of 1:60 to 1:90, the black sesame and water are heated at a temperature of 100 to 120 ° C. for 4 to 6 hours so that the ratio is 1:20 to 1:30, followed by extraction for the fourth step. Mix the extracted extract from each of the above four rounds, and use the vacuum rotary evaporator to mix the concentrate and the extract in a ratio of 1:10 to 1:40 by weight at a temperature of 10-40 ° C. for 10-40 minutes. Characterized in that the manufacturing to the concentration,
The lily extract is sealed in a sealed glass container mixed with a lily and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then 3-8 in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius. The primary leaching and extraction were carried out, followed by the extract, and the leached and extracted primary liquor and alcoholic alcohol 25% spirits were sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 to the weight of 4-10 degrees C. To extract the second leaching for 3-8 weeks in a cool dark place that can avoid the temperature and sunlight of the solution, pour out the extract, leaching the second extraction lily and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ 1: 90 After mixing in the ratio of 100 ~ 120 ℃ the lily and water in a ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours in a water bath to extract the third extract, poured into a strong acid water bath 3 The extracted lily and the pH10.5-11.5 strongly alkaline electrolyzed water in a ratio of 1:60 to 1:90 by weight After mixing to 100 ~ 120 ℃ at a temperature of 100 ~ 120 ℃ the ratio of 1:20 ~ 1:30 in a 4 ~ 6 hours in a water bath to extract the fourth step to extract the extract, from above each of the fourth Mixing the extract and using a vacuum rotary evaporator is characterized in that for 10 to 10 minutes to prepare a concentration of the concentrate and the extract in a ratio of 1:10 to 1:40 by weight in a temperature setting condition of 10-40 ℃, characterized in that
The situation mushroom extract is sealed in a sealed glass container mixed with a 95% alcohol content of the situation mushrooms and alcohol in a ratio of 1: 5 to 1:20, and then in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius After the first leaching extraction for 3-8 weeks, pour out the extract, and then rinse the firstly extracted situation mushroom and alcoholic beverage 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed glass container. Compared to the weight of the situation mushroom and pH2.5-3.5 strongly acidic electrolyzed water extracted by distilling and extracting the secondary extract for 2 to 8 weeks in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius. After mixing at a ratio of 1:60 to 1:90, the situation mushroom and water were heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. for 4 to 6 hours, and extracted in a third manner. Sichuan mushroom and pH10.5-11.5 strongly alkaline electrolyzed water After mixing at a ratio of 1:60 to 1:90 to the weight of 100% to 120 ℃ temperature of the mushrooms and water so that the ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours to be extracted in the fourth Extract the extract, mix the extract extracted from each of the above 4 times, and use the vacuum rotary evaporator to make the concentrate and the extract at a ratio of 1:10 to 1:40 by weight at a temperature of 10-40 degrees Celsius. It is characterized in that to prepare for 10-40 minutes to concentrate, the extract of the root is 4-10 degrees after sealing the sealed airtight glass container with the ratio of 1: 5 ~ 1:20 weight ratio of the weight of the root and alcohol 95% Let the primary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the seed, pour out the extract, leaching and extracting the first extract of the root and alcohol content 25% 1: 5 ~ 1: 20 Sealed in a sealed glass container at a ratio of 4-10 degrees Celsius and avoid sunlight After the second leaching and extraction in the cool and dark place for 3-8 weeks, the extract was poured out, and the leached and extracted secondary roots and the pH2.5-3.5 strongly acidic electrolyzed water were mixed at a ratio of 1:60 to 1:90 by weight. At the temperature of 100-120 ° C., the roots and the water are boiled for 3 to 4 hours in a ratio of 1:20 to 1:30, followed by extraction in the third step, followed by the extract. 5-11.5 After mixing strongly alkaline electrolyzed water at a ratio of 1:60 to 1:90, the root and water in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. for 4 to 6 hours To extract the extract 4 times, followed by extracting the extract from each of the above 4 times, and using the vacuum rotary evaporator at a temperature of 10-40 degrees Celsius setting the concentrated solution and the extract to the weight 1:10 ~ 1 It is characterized in that it is prepared by concentrating for 10-40 minutes to a ratio of 40,
The ginseng extract is sealed in a sealed glass container mixed with ginseng and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. After 8 weeks of primary leaching extraction, the extract was drained, and then the leached and extracted primary ginseng and alcoholic alcohol 25% spirits were sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunshine of the seed, pour out the extract, leaching the extracted second ginseng and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the ginseng and water are heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C for 4 to 6 hours to be extracted in a third manner, followed by extracting the extract, and a strong acidic water. Ratio of 1: 60 ~ 1: 90 to the weight of Gosam and tertiary extracted Alkaline ginseng and pH10.5-11.5 strongly alkaline electrolyzed water After mixing to 100 ~ 120 ℃ the ginseng and water in a ratio of 1:20 ~ 1:30 in 4 to 6 hours in a water bath so that the extract is extracted 4 times, from above each of the fourth round The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,
The Centella asiatica extract is sealed in a hermetically sealed glass container in a ratio of 1: 5 to 1:20 by weight ratio of 95% alcohol and alcohol in a bottle, and then cooled in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained, and the leaching and extracting the primary grass and alcoholic beverage 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 Leak extraction for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching second extraction centrifugal grass and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the centella and the water at a temperature of 100 ~ 120 ℃ 4 to 6 hours so that the ratio of 1:20 ~ 1:30 so that it is extracted in the third step to extract the extract, strong acidic water Ratio of 1: 60 ~ 1: 90 to the weight of the centrifugal grass extracted from the bath and the pH10.5-11.5 strong alkaline electrolyzed water After mixing at 100 ~ 120 ℃ temperature of the centrifugal grass and water in a ratio of 1:20 ~ 1:30 in 4 to 6 hours to be extracted in 4th to extract the extract, from above each 4 times The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,
The pomegranate extract is sealed in a sealed glass container mixed with pomegranate and alcoholic alcohol 95% alcohol in a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place to avoid temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained, and the leached and extracted primary pomegranate and alcoholic alcohol 25% spirits are sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the second extraction pomegranate and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing in a ratio of 1:90, the pomegranate and water are heated at 100-120 ° C. for 4-6 hours in a ratio of 1: 20-1: 30, followed by extraction in 3rd order, followed by the extract, and strongly acidic water. The ratio of pomegranate extracted from the water bath and the pH10.5-11.5 strong alkaline electrolyzed water in the ratio of 1:60 to 1:90 After mixing to 100 ~ 120 ℃ temperature of the pomegranate and water in a ratio of 1:20 ~ 1:30 in 4 to 6 hours to be extracted in 4th to extract the extract, from above each of the fourth The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,
The Angelica extract is sealed in an airtight glass container mixed in the ratio of 1: 5 to 1:20 weight ratio of alcohol and alcohol in the ratio of 1: 5 to 1:20, and then 3-8 in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius 1 weekly leach extraction, drain the extract, and then leaching and extracting the primary extract and alcoholic alcohol 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 degrees To extract the second leaching for 3-8 weeks in a cool dark place that can avoid the temperature and sunlight, pour out the extract, leaching the extracted Angelica and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ 1: 90 After mixing in the ratio of 100 ~ 120 ℃ temperature and the water in the ratio of 1:20 ~ 1:30 in the ratio of 1: 20 ~ 1: 30 in a water bath to extract the third to pour the extract, the strong acid water water bath 3 Extracted Angelica and pH10.5-11.5 strongly alkaline electrolyzed water at a ratio of 1:60 to 1:90 After mixing to 100 ~ 120 ℃ in the temperature of 100 ~ 120 ℃ the ratio of 1:20 ~ 1:30 in a 4 ~ 6 hours in a water bath to extract the fourth step to extract the extract, from above each of the fourth Mixing the extract and using a vacuum rotary evaporator is characterized in that for 10 to 10 minutes to prepare a concentration of the concentrate and the extract in a ratio of 1:10 to 1:40 by weight in a temperature setting condition of 10-40 ℃, characterized in that
The Astragalus extract is sealed in an airtight glass container mixed with a ratio of 1: 5 to 1:20 of Astragalus and alcoholic alcohol at 95% alcohol by weight, and then cooled in a cool dark place that can avoid temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained and the leaching and primary extraction of sulfur and alcoholic alcohol 25% alcohol is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the secondary extract and pH2.5-3.5 strongly acidic electrolyzed water by weight 1: 60 ~ After mixing at a ratio of 1:90, the sulfuric acid and water are heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. for 4 to 6 hours to be extracted in a third manner, followed by extracting the extract, and a strong acidic water. Ratio of 1:60 to 1:90 ratio of the weight of sulfuric acid and pH10.5-11.5 strong alkaline electrolyzed water After mixing to 100 to 120 ℃ the sulfur and water in a ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours in a hot water to extract the fourth step, the extract is poured over each of the fourth The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight. ,
The pogongyoung extract is sealed in a closed glass container mixed with poongongyoung and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. Let the primary leaching extract for 8 weeks, drain the extract, seal the pogongyoung and alcohol distilled liquor 25% alcohol in the ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container 4-10 Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the pogongyoung and pH2.5-3.5 strongly acidic electrolyzed water extracted from 1: 60 ~ After mixing at a ratio of 1:90, the pogongyoung and water at a temperature of 100 ~ 120 ℃ 4 to 6 hours so that the ratio of 1:20 ~ 1:30 to be extracted in a third to extract the extract, strong acidic water Pogongyoung and pH10.5-11.5 strongly alkaline electrolyzed water The mixture is mixed at a ratio of 1:60 to 1:90, followed by 4 to 6 hours of boiling water at a temperature of 100 to 120 ° C. so that the poongyoung and water are at a ratio of 1:20 to 1:30. The extracts were extracted from each of the above four rounds, and the concentrates and extracts were mixed at a temperature of 10-40 degrees Celsius using a vacuum rotary evaporator at a ratio of 1:10 to 1:40 by weight. Characterized in that the concentration is prepared for a minute,
The wolfberry extract is sealed in a sealed glass container mixed with a wolfberry and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then in a cold dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius 3- After 8 weeks of primary leaching extraction, the extract is poured out, and the leached primary extract and alcoholic beverage 25% spirits are sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. The leaching and extraction of the extract from the cool and dark place to avoid the temperature and sunshine of 3 degrees for 8 weeks, drained the extract, leaching the second extraction Gojija and pH2.5-3.5 strongly acidic electrolyzed water 1:60 ~ After mixing at a ratio of 1:90, the Goji and water at a temperature of 100 ~ 120 ℃ 4 to 6 hours so that the ratio of 1:20 ~ 1:30 to be extracted in a third to extract the extract, strong acidic water Comparison of Goji Goji Extracted with Water Bath and pH10.5-11.5 Strongly Alkaline Electrolyzed Water After mixing at a ratio of 1:60 to 1:90, the mixture of the Goji berries and water at a temperature of 100 to 120 ° C. for 4 to 6 hours in a ratio of 1:20 to 1:30 is extracted for the fourth time, and the extract is extracted. The extracts were extracted from each of the above four rounds, and the concentrates and extracts were mixed at a temperature of 10-40 degrees Celsius using a vacuum rotary evaporator at a ratio of 1:10 to 1:40 by weight. Characterized in that the concentration is prepared for a minute,
The ginseng extract is sealed in a sealed glass container mixed with ginseng and alcohol content of 95% alcohol at a ratio of 1: 5 to 1:20 by weight, and then cooled in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract is drained, and the leaching and primary extraction of ginseng and alcohol 25% alcohol is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the seed, rinse the extract, leaching the extracted second ginseng and pH2.5-3.5 strongly acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the ginseng and water are heated in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C for 4 to 6 hours, followed by extraction in 3rd order, followed by extracting the extract, and a strong acidic water. The ratio of ginseng extracted from the water bath and the pH10.5-11.5 strongly alkaline electrolyzed water to the weight ratio of 1:60 to 1:90 After mixing to 100 ~ 120 ℃ the ginseng and water at a temperature of 1:20 ~ 1:30 in a ratio of 4 to 6 hours to be extracted in 4th to extract the extract, and from above each 4 times Mixing the extracted extract and using a vacuum rotary evaporator to prepare a concentration of 10 to 40 minutes to a concentration of 1:10 ~ 1:40 to weight ratio of the concentrate and the extract at a temperature setting of 10-40 ° C. Method for producing antibacterial antioxidant natural sap using natural extracts such as octopus testis.

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