KR20100001238A - Processing method of squid dha/epa and hurb antioxidant moisturing liquid for wrinkles care and the liquid thereof - Google Patents

Abstract

PURPOSE: A method for producing moisturizing sap to smooth wrinkle is provided to easily produce the sap using cuttlefish with low price. CONSTITUTION: A method for producing antioxidative funational moisturizing sap to smooth wrinkle comprises: a step of removing anus of cuttlefish and selecting internal organ of the cuttlefish; a step of mixing the organ with plant oil and pulverizing; a step of heating the pulverized organ and oil mixture with Short Path Distillation Plant at 160-200°C for 60 to 100 minutes; a step of isolating and purifying DHA and EPA; a step of analyzing the DHA/EPA through gas chromatography and removing smell; a step of adding tackifier, moisturizing agent, herb extract, and filtered DHA/EPA and stirring at 50-70°C for 10 to 15 minutes to obtain viscosity sap; and a step of sealing the viscosity sap in a container and rapidly freezing at 18°C.

Description

PROCESSING METHOD OF SQUID DHA / EPA AND HURB ANTIOXIDANT MOISTURING LIQUID FOR WRINKLES CARE AND THE LIQUID THEREOF}

The present invention relates to a method for preparing sap for wrinkle improvement by mixing and stirring using DHA / EPA extracted from squid guts and extracts from natural herbs, thickeners, moisturizers and other antioxidant functional additives, and more specifically, squid DHA / EPA extracted from the squid gut (the ink bag, gills, heart, visceral sac), a byproduct, is added with natural herbal extracts, thickeners, moisturizers, and other antioxidants to help improve skin wrinkles. Thus, the present invention relates to a method for preparing a moisturizing sap for wrinkle improvement using squid by-product extraction DHA EPA and natural herbal extract composition, and sap prepared accordingly.

Skin is the largest organ in the human body, its size is about 1.6㎡, and it is a large organ that weighs 16% of its weight by weight. The skin serves as the most basic skin covering the body to protect internal organs. In addition, it is a very important institution that performs many functions. The skin is in constant contact with changes in the external environment such as ultraviolet rays, temperature changes, moisture, dust, and air pollution. During the contact process, the skin reads the information essential for survival and allows the body to react appropriately to protect the individual. It has an important task and has additional functions to protect the skin itself to some extent from external harm.

However, as we get older, our skin cannot deny the inherent loss of its own function, and in order to supplement it, we have to borrow the power of cosmetics following the consumption of food.

According to the National Fisheries Research and Development Institute, squid contains protein, ash, calcium, phosphorus, etc. It is richer in protein than meat meat, has good digestibility, and contains a large amount of minerals such as vitamins and selenium.

Omega-3 fatty acid means DHA (Docosa Hexaenoic Acid), EPA (Eiocosa Pentaenoic Acid), or DPA (Docosa Pentaenoic Acid).

DHA is a constituent of the brain and retina, which also helps to nourish the brain. EPA helps to improve cholesterol and blood circulation, and DPA is an essential nutrient for breast growth. Omega-3 fatty acids, a type of unsaturated fatty acids, are essential fatty acids that are not produced by the body and can only be obtained by eating food.

Therefore, if you look at the functionality of the product containing DHA EPA, the first improvement of blood cholesterol. Blood cholesterol is elevated due to excessive intake of animal fat, lack of exercise, and obesity smoking stress. Omega-3 fatty acids, which are a type of unsaturated fatty acids, are essential fatty acids that help the body improve cholesterol. Next helps with blood circulation. Since the blood of our body plays the most important role in life, of course, the blood flows well, all functions are smooth. And nutrition of the brain and retina.

Because DHA is a constituent of the brain and retina, it is an essential fatty acid that nourishes the retina, the brain, nervous system and eye tissue. Squid fatty acids contain 15.8% and 37.1% polyunsaturated fatty acids, such as DHA EPA, which are not found in normal meat, respectively. These unsaturated fatty acids prevent low-density lipoprotein cholesterol (LDL) from accumulating in the blood vessels. It is an essential amino acid that prevents geriatric diseases, improves brain function, and improves wrinkles by activating cells through antioxidant activity.It is extracted and purified and emulsified and used in the cosmetic industry to absorb moisture through the skin. Research is actively underway.

In addition, researches and product launches of natural cosmetics made by mixing natural herbal extracts and antioxidant functional additives to help improve wrinkles and skin elasticity have been actively conducted.

Currently, functional cosmetics in Korea are categorized into three categories: wrinkle improvement products, whitening products, and sunscreen inhibitors, which are recognized by the Korea Food and Drug Administration through examination of functional cosmetics. As a product for improving functional anti-wrinkle with the addition of a functional composition, it is still being developed and marketed, and relatively expensive products are the mainstream.

Therefore, the present invention uses a natural herbal extract composition, a thickener, a moisturizer, and other antioxidants that help to improve skin wrinkles of DHAEPA extracted from the internal organs, which is a squid by-product, and sap that can help improve wrinkles. It relates to a manufacturing method, which can meet the preference of elderly consumers who want to supplement skin aging and lack of elasticity and elasticity with age, as well as general consumers who need skin moisturizing, and also moisturizing to be stored frozen for a long time Method for producing moisturizing sap for wrinkle improvement using DHAEPA and natural herb extract composition extracted from internal organs, a squid by-product that can be mass-produced and distributed for sale. And the sap prepared accordingly.

The present invention achieves the above object is a pre-treatment step of screening and washing and cleaning the internal organs of the squid body parts remaining after separating and removing the anal portion; A viscera crushing step of crushing the pretreated squid viscera with vegetable oil; ( The mixing ratio of vegetable oil and gut ?) By using the short path distillation plant, the ground gut and oil are heated at a temperature of 160-200 ° C for 60-100 minutes to utilize the boiling point of DHA / EPA itself. Separation and purification step of separating and purifying the DHA and EPA by; Filtration step of filtering the purified purified DHA / EPA using activated gas chromatography (Gas Chromatography) to determine the purity and remove the odor by qualitative and quantitative; Stirring manufacturing process for producing a viscous sap by adding the filtered DHA / EPA, natural herb extracts, thickeners and moisturizer with a homogenizer for 10-15 minutes at a temperature condition of 50-70 degrees Celsius; Squid comprising a, (? What is the reference material for mixing the material), and sealing packaging process of the capture the produced viscous fluid in the container sealing packing and rapid freezing of the sealed product to a core temperature below minus 18 ℃ By-product extraction DHA / EPA and natural herbal extracts to provide a method for the production of antioxidant functional moisturizing sap for wrinkle improvement.

In the present invention, the pretreatment process is washed with running water such as foreign substances and dirt stained on the squid intestines, pH10.5-11.5 alkaline electrolyzed water and pH2.5-3.5 of 2-5 times the weight of the intestines with immersion liquid Each acidic electrolyzed water was prepared and compared to a house level in an electrolytic water tank, and firstly, the washed internal organs were immersed in the alkaline electrolyzed water immersion tank for 5-15 minutes, and secondly, immersed in the acidic electrolytic water immersion tank for 5-15 minutes. To provide a method for producing an antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA EPA and natural herbal extracts.

In another aspect, the present invention provides a method for producing antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA / EPA and natural herbal extracts, characterized in that the vegetable oil is selected from the group consisting of ().

In another aspect, the present invention provides a method for producing an antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA / EPA and natural herbal extracts, characterized in that the functional additive is further added in the stirring manufacturing process.

In another aspect, the present invention provides a method for producing an antioxidant functional moisturizing sap solution for wrinkle improvement using squid by-product extract DHA / EPA and natural herbal extracts, characterized in that the purity of the purified purified DHA / EPA in the separation and purification process is 12-15%. to provide.

In addition, the present invention is the squid by-product extract DHA / EPA and natural herb extracts, characterized in that the mixing ratio of the DHA / EPA and natural herb extracts, thickeners and moisturizers are 0.1-10% of the total amount of sap It provides a method for preparing an antioxidant functional moisturizing solution.

In addition, the present invention, the natural herb extract is bokbunja extract, Angelica extract, rounde extract, Astragalus extract, brown root extract, sesame extract, cucumber extract, mulberry extract, mugwort extract, ginkgo biloba extract, green tea extract, high ginseng extract, licorice extract, blueberry Extract, Indongcho Extract, Carrot Extract, Tomato Extract, Red Ginseng Extract, Propolis Extract, Grapefruit Seed Extract, Rose Hip Extract, Lavender Extract, Rosemary Extract, Chamomile Extract, Calendula Extract, Rose Extract, Nettle Extract, Basil Extract, Time Extract Provided is a method for preparing antioxidant functional moisturizing sap solution for wrinkle improvement using squid by-product extract DHA / EPA and natural herb extract, characterized in that the mixture is one or two or more selected from the group.

In the present invention, the thickener is wrinkles using squid by-product extraction DHA / EPA and natural herbal extracts, characterized in that the mixture is one or two or more selected from the group consisting of sodium alginate, carrageenan, pectin, guar gum, xanthan gum It provides a method for producing an antioxidant functional moisturizing solution for improvement.

In addition, the present invention, the moisturizing agent is one or a mixture of two or more selected from the group consisting of hyaluronic acid, butylene glycol, glycerin, sorbitol, placenter, elastin, moistin, lipidur, natural betaine, propolis, ceramide Squid by-product extract DHA / EPA and natural herbal extracts characterized in that the present invention provides a method for producing an antioxidant functional moisturizing sap for wrinkle improvement.

In the present invention, the functional additive is added 0.1-10.0% to the total weight of the sap, squid by-products, characterized in that one or two or more selected from the group consisting of tocopherol, ascorbic acid, coenzyme Q10, retinol, panthenol It provides a method for producing an antioxidant functional moisturizing sap for wrinkle improvement using the extracted DHA / EPA and natural herbal extracts.

In addition, the present invention, the natural herb extracts each extract raw material and alcohol content 95% spirits in a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container in a ratio of 4-10 degrees Celsius and sunlight The primary leaching extract for 3-8 weeks in a cool dark place to avoid the bleeding, extract the extract, leaching the primary extract and mixed alcohol 25% alcohol at a ratio of 1: 5 to 1:20 by weight After sealing in a glass container, the secondary leaching extract for 3-8 weeks in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees, pour out the extract, leaching the second extract and pH2.5-3.5 Strong acidic electrolyzed water is mixed at a ratio of 1:60 to 1:90 by weight, and then extracted at a temperature of 100 to 120 ° C. for 4 to 6 hours so that the raw material and water are at a ratio of 1:20 to 1:30. The extracted solution was poured out, and the raw material extracted with tertiary water bath in strong acid water and pH 10.5-11.5 strong alkaline electrolyte After mixing the water at a ratio of 1:60 to 1:90 to the weight, the extract and the water at a temperature of 100 to 120 ° C. are soaked in a ratio of 1:20 to 1:30 for 4 to 6 hours to be extracted in the fourth order. The extract is poured out, and the extracts are extracted from each of the 4th stages above, and the concentrate and the extracts are 1:10 to 1:40 by weight using a vacuum rotary evaporator at a temperature of 10-40 degrees Celsius. It provides a method for producing antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA / EPA and natural herbal extracts, characterized in that the concentration is prepared for 10-40 minutes.

In another aspect, the present invention provides an antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA / EPA and natural herbal extracts, characterized in that it is prepared by any one of the manufacturing method described above.

According to the manufacturing method of antioxidant functional sap for wrinkle improvement using the squid by-product extraction DHAEPA and natural herb extract composition of the present invention as described above, there is no big obstacle in supplying raw materials of internal organs which are squid by-products in the pretreatment step Squid by-product extraction DHAEPA and natural herb extract composition that can be easily understood and immediately checked for easy recovery, maintenance and adaptation of skin elasticity to anyone through easy absorption of functional sap coating, along with increased awareness of antioxidant skin care that has been neglected It provides a method of producing an antioxidant functional sap for wrinkle improvement using.

In addition, various viscosity modifiers and moisturizer additives are added to the functional sap to provide a moist and soft touch when absorbed into the skin.It is an extract of various kinds of aromas, herbs, herbs, etc. As it is possible to manufacture mixed formulations of medical prescription pharmaceutical solutions such as anti-inflammatory agents, it is possible to formulate and manufacture various high value-added products to provide a breakthrough that provides the advantages of squid by-product extraction using DHA EPA and natural herb extract composition. It provides a method for producing an antioxidant functional moisturizing sap for wrinkle improvement.

The technical problems achieved by the present invention and the practice of the present invention will be made clear by the following preferred embodiments of the present invention. The following examples are merely illustrated to illustrate the present invention and are not intended to limit the scope of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings and experimental examples.

1 is a flow chart illustrating a method for preparing an antioxidant functional moisturizing sap solution for wrinkle improvement using squid by-product extraction DHAEPA and natural herb extract composition according to an embodiment of the present invention. Referring to the drawings, the pre-treatment step (S11) to collect the squid and squid to collect the internal organs of the squid body and to separate and clean the remaining internal organs (black ink bag, gills, heart, internal organs) after separating and removing the anal part (S11); Internal organ grinding process (S12) for mixing and grinding the pretreated squid internal organs in the vegetable mixed oil; Separating and refining step of separating and purifying DHAEPA with purity of 12-15% by using boiling point of DHAEPA itself by heating heat from 160-200 ° C for 60-100 minutes using a molecular distillation device (ShortPathDistillationPlant) (S13); Filtration step of separating purified DHAEPA using gas chromatography (GasChromatography) qualitative and quantitative to check the purity and to remove the odor filtered on activated carbon (S14); Agitated manufacturing step (S15) of preparing a viscous sap by adding filtered high DHAEPA and natural herb extracts, thickeners and moisturizers and functional additives; Sealing packaging step (S16) to put the prepared sap in a container such as PE and PP sealing sealing; consists of.

In particular, the production method of antioxidant functional sap for wrinkle improvement using squid by-product extraction DHAEPA and natural herb extract composition according to an embodiment of the present invention is washed by first immersing the intestines in alkaline electrolytic water immersion solution for 5-15 minutes in the pretreatment step Since it is washed and then sterilized by immersing it in acidic electrolytic water immersion solution for 5-15 minutes, it is necessary to prepare electrolytic water for preparation of immersion liquid. The same amount of strong acid electrolytic water and strong alkaline electrolyzed water generated by electrolyzing drinking water is collected. All of the generated strong electric water can be effectively utilized by preparing the immersion liquid for each purpose.

In addition, the viscosity adjusting agent and the moisturizing agent added in the stirring manufacturing step of the massage solution can be prepared for the sap to enable long-term cryopreservation by inhibiting freezing degeneration.

In more detail, the above-described pretreatment step (S11) is a process of collecting tap water drinking water, which is a raw material, and electrolyzing it in an electrolytic cell to prepare and use the produced alkaline electrolytic water and acid electrolytic water as an immersion water solution. It is a process of removing and washing the intestinal filth and foreign substances, which are the food parts, and collecting them.

When the water temperature of the electrolyte is increased during the production of the electrolytic water, the ionic change is activated in the diaphragm in the electrolytic cell, so that the strong acid electrolytic water generated at the anode not only increases the concentration of hypochlorite (HOCL), an effective chlorine which has a bactericidal effect, but also the strong alkali generated at the cathode. Electrolyzed water has a high concentration of sodium hydroxide (NaOH) having an interfacial effect, so that an underwater heater, which is a heating facility, is installed in a distilled water reservoir to operate the water temperature at 35-40 degrees Celsius.

In addition, in the above-mentioned electrolysis process, 0.1-10.0% sodium chloride (NACL) is added to distilled water, mixed dilution to make an electrolyte, and then electrolyzed in the electrolytic cell by a diaphragm electrolyte tank. Exhaust pipes are installed to allow the discharge of chlorine gas from the anode.

In general, electro-analysised water refers to water obtained by applying electric force to general water such as tap water or ground water. If the positive and negative platinum-plated titanium is put into the water and the electricity is supplied by direct current, the anion dissolved in the water is collected on the positive electrode side, and acidic ionized water is collected on the positive electrode side. Occurs. The electrolyzed water thus produced has its ionic effect for 7-8 days when exposed to air and about 3-4 weeks when stored with the lid closed.

In the process of collecting the strongly alkaline electrolyzed water and the strongly acidic electrolyzed water generated in the above-mentioned electrolytic water collection process for use as the strongly alkaline pretreated solution and the strongly acidic pretreated solution, respectively, the strongly acidic electrolytic water on the positive electrode side and the strong alkaline on the negative electrode side are collected. Collect the electrolyzed water separately and put it in the sump and circulate to maintain the water temperature at 5-10 degrees Celsius by using chiller which is a cooling facility in each sump. Underwater UV sterilizers are installed in each water tank to sterilize them so that the pH properties are unchanged.

Also, care should be taken to prevent re-contamination of each collected ionized water. In case of acidic ionized water, it has a converging effect and constricts the skin.

Therefore, since it protects the skin slightly acidic, it is of course excellent in skin beauty and disinfection.

Hypochlorite Water Additive Standard of Korea Food and Drug Administration and Hypochlorite Water Food Additive Approval Standard of Ministry of Health, Labor and Welfare of Japan Ministry of Health, Welfare and Hypochlorous Acid Water (Also called strongly acidic hypochlorous acid) is less than pH2.7 and contains 20-60ppm of hypochlorous acid (HOCL), an effective chlorine, and non-acidic water (also called non-acidic hypochlorous acid) is pH5.0-6.5 It contains 10-30ppm of hypochlorous acid (HOCL), and 10ppm of effective chlorine has a bactericidal effect against bacteria with low disinfectant resistance, and 20-30ppm of effective chlorine has a bactericidal effect against bacteria that do not have apopoies. An effective chlorine of 60ppm has a bactericidal effect within minutes of infant bacilli.

Sodium hypochlorite water is not effective in killing follicles, but strong acid hypochlorite is a very effective fungicide for follicles and has bactericidal effects within 3 minutes of treatment with Bacillus and clostridium.

Hypochlorite water is characterized by a strong bactericidal and deodorizing effect and very low biotoxicity. The bactericidal power is fungi, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and other nutrients, Cereus, Antibacterial, Fungi, Viruses, Heterogeneous bacteria, Salmonella It has strong bactericidal power against food poisoning such as bacteria and Vibrio bacteria, as well as all pathogenic microorganisms caused by raw water. (Source: Less than 5 seconds of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and other trophic bacteria, less than 5 minutes , Antibacterial 1 min-2 min 30 sec, Fungus 5 sec-1 min, Virus less than 5 sec, Antimicrobial effect of strong acidic electrolyzed water, Clinical test, 37: 919-919,1993) Salmonella and Vibrio bacteria were detected at 120,000-200,000 bacteria after 30 seconds of treatment, Sterilization test report, Korea Testing and Research Institute, 2002)

Hypochlorite water is a fundamental countermeasure for sterilization, it has a weak odor and odor, and is experimentally 80 times stronger than sodium hypochlorite (NAOCL) and 5-10 times stronger in practice, so the effect is hypochlorous acid with 30 ppm chlorine concentration. The sterilizing power of water is superior to sodium hypochlorite water (chlorine diluent), which has a chlorine concentration of 150 ppm, and also has excellent deodorizing power. The FDA also considers the biotoxicity of hypochlorite water. Is recognized as 1/50 of sodium hypochlorite, so it is already widely used as a substitute for chemical disinfectants and pesticides.

Hypochlorite water is already used as an optimal disinfectant for airborne poisoning due to water, food raw materials, and spray because it is free of odors and does not contain salts. Sterilization is increased by increasing the temperature of hypochlorous acid.

Strong alkaline ionized water is generated on the opposite (-) side as much as the amount of strong acidic water produced on the hypochlorite solution manufacturing device (+), and serves as a surfactant, so strong alkaline ionized water is used as a cleaning agent, as well as chemicals such as wax and chemical fertilizer. It is also used as a substitute.

In addition, alkaline ionized water has more beneficial minerals (inorganic salts) than normal water, and makes water molecules smaller, so the absorption of minerals is increased, which helps improve diarrhea and constipation, and works on the stomach.

Strong alkaline ionized water is a natural emulsifier with excellent dissolving and detergency, as well as a surfactant having excellent natural surfactant effect containing 40-120 ppm of sodium hydroxide (NaOH) at pH11.0 or higher. As it has physical properties and functions to improve cleaning power, it is mainly used for cleaning raw foods such as root vegetables (potatoes, potatoes, carrots, etc.), fruit vegetables (cucumbers, pumpkins, tomatoes, etc.) and grains (beans, brown rice, etc.). .

In foreign countries, strong alkaline water with a pH greater than 11.0 is recognized as a dilution solution of sodium hydroxide solution. It contains sodium hydroxide and natural emulsifiers and functions as a non-toxic natural detergent. It is due to the effect of washing off grease well. The cleaning power is increased by increasing the temperature of the strong alkaline water.

In the case of countries where electrolyzed water became legislated into strong acidic and strong alkaline ionized water, Kangsan Electrolyzed Water was announced in Korea in December 2005 by the Korea Food & Drug Administration (Packaging Container Team) No. 2005-75. It was first used as a standard and standard for hypochlorite, which is a disinfectant for facilities, and was announced as No. 2007-74 of the Korea Food and Drug Administration (Food Additives Team) in November 2007. And specifications.

Foreign cases preceded us. In June 2002, the Ministry of Health, Labor and Welfare Ordinance No. 75 and Notice No. 212 recognized strong acidic water as hypochlorous acid and approved as a food additive (sterilizer) in China. In 1999, the sanitary power generation device was approved by the Ministry of Sanitation as a disinfection device, and in 1998, the EPA (Environmental Protection Agency) was approved by the US Department of Environmental Protection (EPA) as a disinfection manufacturing device. Recognized as the same as hypochlorous acid water and approved as a disinfectant for food materials and utensils containers in the same year, USDA (Ministry of Agriculture) approved the use of electrolytic hypochlorous acid for the purpose of killing intestinal pathogens of growth.

In foreign countries, since 1996, in Japan, hand sterilization, endoscopy disinfection, antibacterial measures, hospital infection prevention, burn treatment, oral disinfection, etc. In 2002, it was used for the purpose of maintaining the freshness of harvest, and in 2002, it was used in the environmental sector to remove organic odors, to decompose pollutants, to manufacture drinking water, and to add food additives (pasteurizers), cooking processing facilities, utensil containers, Its use has been expanded to improve the quality and conservation of strong alkaline electrolyzed water.

Therefore, strong acidic electrolyzed water is a product that is approved for use in food hygiene not only in Korea but also in the world. It is harmless to humans, has no residue, and has excellent sterilizing power as can be seen from the above examples. .

As described above, the characteristics of each strong electric seawater are summarized first, and then the strong alkaline electrolyzed water should be cleaned and then sterilized and disinfected with strong acidic hypochlorite water. If the object is first immersed in strong alkaline electrolyzed water to remove organic contaminants and washed and disinfected with strong acidic electrolyzed water, the sterilization effect can be expected to be greatly increased.

Therefore, in the present invention, by setting the squid internal organs immersed in strong alkaline electrolyzed water and sterilized by sterilizing by immersing in strong acidic electrolyzed water, rinsing and sterilization are simultaneously performed to maximize the effect.

In more detail, the above-described pretreatment step (S11) is a process of squid squid to sterilize by-product squid internal organs in the root and alkaline electrolyzed water to wash and wash and sterilize by immersion in acid temple seawater.

In general, raw materials can use all kinds of squid, such as domestic coast, Peru, Mexico, North Pacific.

In addition, squid is a dried or specially processed biological squid that can be used both frozen and alive squid. In case of thawing frozen squid, thaw without immersion in refrigerated thawing room with the outside temperature below minus 5 ℃ and ventilate with a fan for quick thawing.

The cuttlefish consists of the head, torso, leg part and the invisible part, eyes, bones, teeth, and intestines, so that the intestinal part of the body is collected and the anal part is separated and removed. , Gills, heart, visceral sac), collected and weighed and washed in running water of foreign substances and dirt, etc. buried in the internal organs, pH10.5-11.5 alkaline electrolyzed water and pH2 with immersion liquid 2-5 times the weight of the internal organs. Prepare 5-3.5 acidic electrolyzed water separately and immerse in alkaline electrolytic water immersion tank for 5-15 minutes first, and immersion in acidic electrolytic immersion water tank for 5-15 minutes.

In this case, for hygiene, work table, knife and cutting board should be disinfected from 100 ~ 200ppm solution or 20 ~ 25ppm solution of iodine at any time.

In the above-mentioned internal organ grinding step (S12), the internal organs, which are pretreated squid by-products, are collected and ground into 0.1 to 2.0 mm using a grinding machine. In this case, the food contact surface around the blade or the work surface of the fine cutter is alcohol. It should be sterilized frequently using% solution.

Through the process of mixing crushed squid viscera in the invisible area with vegetable mixed oil, the mixed vegetable oil penetrates well into the squid viscera and mixes it evenly so that DHA EPA in the viscera is mixed with vegetable mixed oil in oil and leached and leached. The composition of vegetable mixed oil is 10-300% macadamia oil by weight of squid internal organ, 10-300% jojoba oil by weight of squid internal organ, 10-300% jojoba gold oil by weight of squid internal organ, squid At least one of 10-300% hazlot oil, 10-300% avocado oil, and 10-300% almond oil by weight of squid organ must be mixed and composed. It is a process of preparing a total amount of mixed oil of 100-300% of the weight of the squid intestine and mixing it with the squid intestine and grinding it.

The above-described separation and purification step (S13) uses a molecular distillation apparatus (ShortPathDistillationPlant) to heat heat at 160-200 ° C. for 60-100 minutes to utilize boiling point of DHA EPA itself. Process to separate and purify DHA / EPA.

The filtration step (S14) described above is performed three times on activated carbon to confirm purity and remove odor by qualitative and quantitative determination of DHA EPA using gas purification (GasChromatography) with a purity of 12-15%. It is a process of filtration.

In the aforementioned stirring manufacturing step (S15), a sap for wrinkle improvement is prepared by adding purified and purified DHA EPA, natural herb extract, thickener, humectant, and functional additive using a molecular distillation apparatus and chromatography. Natural herbal extracts and DHA EPA of 0.1-10.0% by weight of sap, sapbun extract of 0.1-10.0% by weight of sap, 0.1-10.0% by weight of sap, and 0.1-10.0 by weight of sap. % Rounde extract, 0.1-10.0% of total amount of sap, astragalus extract, 0.1-10.0% of extract of sap weight, 0.1-10.0% of sap extract weight, 0.1-10.0% of total amount of sap Ogapi extract, 0.1-10.0% of total sap weight, 0.1-10.0% mulberry extract, total sap, 0.1-10.0% mugwort extract, ginkgo leaf extract, 0.1-10.0% total sap, total amount of sap medium Green tea extract of 0.1-10.0%, sap extract of 0.1-10.0% by weight of total sap, licorice extract of 0.1-10.0% by weight of total sap, blueberry extract of 0.1-10.0% by weight of total sap, and sap by weight 0.1-10.0% Indongcho extract, 0.1-10.0% carrot extract by weight of sap total weight, 0.1-10.0% tomato extract by weight of sap total weight, 0.1-10.0% red ginseng extract by sap total weight, 0.1- by weight total sap 10.0% propolis extract, 0.1-10.0% grapefruit seed extract by weight of total sap, 0.1-10.0% rose hip extract by weight of total sap, 0.1-10.0% lavender extract by weight, total sap 0.1- 10.0% rosemary extract, 0.1-10.0% chamomile extract by weight of sap, 0.1-10.0% calendula extract by weight of sap, 0.1-10.0% rose extract by weight of sap, 0.1-10.0% by weight of sap Nettle extract, number Natural herb extracts, such as 0.1-10.0% basil extracts by weight, and 0.1-10.0% thyme extracts by weight of sap, 0.1-10.0% sodium alginate by weight of sap, 0.1-10.0% by weight of sap, Additives such as 0.1-10.0% pectin, 0.1-10.0% gut gum by weight, and 0.1-10.0% xanthan gum by weight of sap, and 0.1-10.0% by weight of sap. Hyaluronic acid, 0.1-10.0% of total amount of sap, butylene glycol, 0.1-10.0% of total amount of sap, glycerol, 0.1-10.0% of total amount of sap, 0.1-10.0% of total amount of sap , 0.1-10.0% of total amount of sap, elastin, 0.1-10.0% of total amount of sap, 0.1-10.0% of repeat amount of total sap, 0.1-10.0% of natural betaine, total amount of sap 0.1-10.0% of total weight of propolis, 0.1-1 of total amount of sap Moisturizing agent such as 0.0% ceramide is added, 0.1-10.0% tocopherol by weight, 0.1-10.0% ascorbic acid by weight, 0.1-10.0% by weight, and 0.1-10.0% by weight Retinol, 0.1-10.0% of total weight by weight of panthenol, such as any one or more of the concentrate is composed of a blended mixture and the final stirring in a homogenizer for 10-15 minutes at a temperature of 50-70 degrees Celsius.

The bokbunja extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 of bokbunja and alcoholic alcohol at a ratio of 1: 5 to 1:20, and then stored in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. -8 weeks to extract the first leaching, pour out the extract, and then leached the first extract bokbunja and alcoholic alcohol 25% alcohol in a ratio of 1: 5 ~ 1:20 by weight in a sealed sealed glass container 4 Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of -10 degrees Celsius, pour out the extract, leaching the second extract Bokbunja and pH2.5-3.5 strong acidic electrolyzed water 1: After mixing at a ratio of 60 to 1:90, the mixture is boiled for 4 to 6 hours at a temperature of 100 to 120 ° C. so that the ratio is 1:20 to 1:30, followed by extraction in a third manner. Ratio of 1: 60 ~ 1: 90 to the weight of Bokbunja and pH10.5-11.5 strong alkaline electrolyzed water After mixing to 100 ~ 120 ℃ at a temperature of 100 ~ 120 ℃ the ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours in a water bath so that the extract is extracted in 4th, from above each 4 times The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight.

The Angelica extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 of alcohol and 95% alcohol content in weight, and then stored in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. First, extract the first leaching for 8 weeks, drain the extract, and seal the primary extract and alcoholic beverage 25% alcohol extracted by leaching at a ratio of 1: 5 to 1:20 by weight in a sealed hermetically sealed glass container. Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of 10 degrees Celsius, extract the extract, leaching the extracted Angelica and pH2.5-3.5 strong acidic electrolyzed water 1:60 After mixing at a ratio of ~ 1: 90, the donkey and water are heated at a temperature of 100 to 120 ° C. for 1 to 30 hours in a ratio of 1:20 to 1:30, and extracted in a third manner, followed by extracting the extract. The mixture of Angelica quinquefolius extracted with Gangsan water bath and the alkaline alkaline electrolyzed water in the ratio of 1: 60 ~ 1: 90 by weight Mix the extract extracted from each of the above, extracting the extract, and then extracting the extract 4 times for 4-6 hours in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. And it is prepared by concentrating 10-40 minutes in a concentration of 1:10 ~ 1:40 to the weight ratio of the concentrate and extract in a setting condition of temperature 10-40 ℃ using a vacuum rotary evaporator.

The rounde extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of alcohol and 95% alcohol, and then in a cool and dark place to avoid the temperature and sunlight of 4-10 degrees Celsius. After 8 weeks of primary leaching extraction, the extract was drained, and the leached primary extract and 25% alcohol content were sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight, and then 4-10. Let the secondary leaching extract for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of the degree, pour out the extract, leaching the secondary extract round and pH2.5-3.5 strong acidic electrolyzed water 1: 60 ~ After mixing at a ratio of 1:90, the roundes and water were heated for 4-6 hours at a temperature of 100 to 120 ° C. so as to be extracted in a third manner, followed by extraction of the extract, and a strong acidic water. The ratio of 1: 60 ~ 1: 90 to the weight of roundle and pH10.5-11.5 strong alkaline electrolyzed water extracted from the water bath After mixing to 100 ~ 120 ℃ the round and water in a ratio of 1:20 ~ 1:30 in a ratio of 4 to 6 hours in a water bath so that the extract is extracted in 4th, from above each 4 times The extracted extract is mixed and prepared by concentrating the extract and the extract at a temperature of 10-40 ° C. using a vacuum rotary evaporator for 10-40 minutes in a ratio of 1:10 to 1:40 by weight.

The sulfur extract is sealed in a mixed sealed glass container in a ratio of 1: 5 to 1:20 of sulfur and alcohol content of 95% alcohol by weight, and then stored in a cool dark place where the temperature and sunlight of 4-10 degrees can be avoided. Extract the extracted liquor by allowing the first leaching to be extracted for 8 weeks, and seal the first extracted sulfur and alcohol with 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a mixed sealed glass container. The secondary leaching extraction is carried out for 3-8 weeks in a cold dark place where the temperature and the temperature of -10 degrees Celsius can be avoided, and the extracted liquid is followed, and the leaching secondary sulfuric acid and pH 2.5-3.5 strongly acidic electrolyzed water by weight is 1:60. After mixing at the ratio of ~ 1: 90, the sulfuric acid and water are extracted in a third time by boiling in 4-6 hours at a temperature of 100 ~ 120 ° C so that the ratio is 1: 20 ~ 1: 30, and the acidic water is extracted. Sulfur extracted from the tertiary bath and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1:60 to 1:90 by weight, and then the sulfur and water were 1:20 to 1:30 at a temperature of 100 to 120 ° C. Ratio Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of temperature 10-40 degrees. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The root extract is sealed in a mixed airtight glass container at a ratio of 1: 5 to 1:20 weight ratio of the root and alcohol in a ratio of 1: 5 to 1:20. After extracting the primary leaching for 8 weeks, pour the extract, and seal the primary root extract and 25% alcohol with alcohol at a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container. Follow the extraction by allowing the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius, avoiding sunlight, and leaching the second extracted brown root and pH 2.5-3.5 strong acidic electrolyzed water by weight 1:60. After mixing in the ratio of ~ 1: 90, the extract and the water are extracted in a third time by boiling in 4 ~ 6 hours so that the root and water are in the ratio of 1: 20 ~ 1: 30 at a temperature of 100 ~ 120 ° C. The mixture of brown roots extracted with the strong acid water bath and the pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1:60 to 1:90, and then at the temperature of 100 to 120 ° C. is 1:20 to 1: 30 rain Extract the extract from the mixture by pouring it in 4 times with 4 to 6 hours of boiling water and mix the extract from each of the above 4 times, and use the vacuum rotary evaporator to extract the concentrate and the extract under the temperature setting of 10-40 degrees. It is prepared by concentrating 10-40 minutes in a ratio of 1:10 to 1:40 to weight.

The larvae extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of larvae and alcohol in a ratio of 1: 5 to 1:20. After extracting the primary leaching for 8 weeks, pour out the extract, and seal the primary extracted larvae and alcohol with 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed, sealed glass container. The secondary leaching extract is allowed to be extracted for 3-8 weeks in a cool and dark place where the temperature is -10 degrees Celsius, and the sunlight is avoided. After mixing at a ratio of ˜1: 90, the extracts are poured into a mixture at a temperature of 100 to 120 ° C. in a ratio of 1:20 to 1:30 for 4 to 6 hours, followed by extraction. After mixing tertiary extracted with strong acidic water bath and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60 ~ 1: 90 to weight ratio, the above two at the temperature of 100 ~ 120 ℃ is 1: 20 ~ 1: The rate of 30 Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrate and extracts under the setting conditions of 10-40 ° C It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The Ogapi extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of Ogapi and an alcohol content of 3% in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. -After 8 weeks of primary leach extraction, extract the extract, seal it and mix it with the sealed hermetically sealed glass container at a ratio of 1: 5 ~ 1: 20 by weight of the extracted Ogapi and Alcohol. Allow the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius, avoiding sunlight, and then leach the second extracted ogapi and pH 2.5-3.5 strong acidic electrolyzed water by weight 1:60 After mixing at a ratio of ˜1: 90, the extracts are poured into a third mixture by hot water for 4 to 6 hours at a temperature of 100 to 120 ° C. so as to have a ratio of 1:20 to 1:30, followed by extraction thereof. The Ogapi extracted from tertiary water bath in strong acidic water and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1:60 to 1:90 by weight, and then the Ogapi and water at a temperature of 100 to 120 ° C. Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight to prepare.

The mulberry extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 of mulberry and 95% alcohol content by weight, and then stored in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees. -After 8 weeks of primary leaching, extract the extract, seal the mulberry and alcohol, and extract 25% alcohol of primary extraction in a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container.4 Follow the extraction by allowing the secondary leaching to be extracted for 3-8 weeks in a cool and dark place that can avoid the temperature of -10 degrees Celsius, and leach the secondary extract of mulberry and pH 2.5-3.5 strong acidic electrolyzed water by weight 1:60. After mixing at a ratio of ~ 1: 90, the mulberry and water are extracted in a third manner by boiling in 4-6 hours so that the mulberry and water are in a ratio of 1: 20-1: 30 at a temperature of 100-120 ° C. After mixing the mulberry tree extracted with tertiary water with strong acidic water bath and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to the weight, the mulberry and water were separated at a temperature of 100-120 ° C. Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight to prepare.

The mugwort extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of mugwort and alcohol, at a ratio of 1: 5 to 1:20. After extracting the primary leaching for 8 weeks, pour out the extract, seal the primary extract of mugwort and alcohol with 25% alcohol in a ratio of 1: 5 to 1:20 to the weight, and seal it in the mixed sealed glass container. The secondary leaching extraction is carried out for 3-8 weeks in a cold dark place where the temperature and the temperature of -10 degrees Celsius are avoided, and the extract is followed. After mixing at the ratio of ~ 1: 90, the mugwort and the water are poured into the third extract by boiling in 4-6 hours at a temperature of 100-120 ° C so that the ratio is 1: 20-1: 30. Mugwort and water were mixed at a temperature of 100-120 ° C. after mixing tertiary extracted mugwort with strong acidic water bath and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1:60 to 1:90. 4-6 hours to be the ratio of 30 The extracts were extracted in 4th order, followed by the extracts, and the extracts were extracted from each of the 4th phases above, and the concentrates and extracts were mixed at a temperature of 10-40 degrees with a vacuum rotary evaporator. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:40.

The ginkgo biloba extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of ginkgo biloba and alcohol, and then stored in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. After extracting the primary leaching for 8 weeks, extract the extract, and then seal the primary extract and extract 25% alcohol from the ginkgo leaf and alcohol in a ratio of 1: 5 to 1:20 to the weight of the mixed sealed glass container. Follow the extraction by allowing the secondary leaching to be carried out for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of -10 degrees Celsius, and leach the secondary extracted ginkgo leaf and pH2.5-3.5 strong acidic electrolyzed water by weight 1:60 After mixing at a ratio of ˜1: 90, at 100-120 ° C., the ginkgo biloba leaves and water may be extracted in a third manner by boiling in 4-6 hours so that the ratio is 1: 20-1: 30. Ginkgo biloba leaves extracted from the water bath in strong acid water and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1:60 to 1:90 by weight, and then the ginkgo biloba leaves and water were heated at a temperature of 100 to 120 ° C. Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight to prepare.

The green tea extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight of green tea and alcohol at a ratio of 1: 5 to 1:20, and then stored in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. After extracting the primary leaching for 8 weeks, pour out the extract, and seal the green tea and alcohol with 25% alcohol extracted by leaching in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight. The secondary leaching extraction is carried out for 3-8 weeks in a cool dark place that can avoid the temperature and sunlight of -10 degrees Celsius, followed by the extraction, and leaching the second extracted green tea and pH 2.5-3.5 strong acidic electrolyzed water by weight 1:60. After mixing at a ratio of ~ 1: 90, the green tea and water are extracted in a third time by pouring in green tea and water in a ratio of 1:20 to 1:30 in a ratio of 1:20 to 1:30, followed by the extract. The green tea and the water extracted with tertiary water bath in strong acidic water and the pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1: 60-1: 90 to the weight, and the green tea and water were 1: 20-1: The rate of 30 Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrate and extracts under the setting conditions of 10-40 ° C It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The ginseng extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of ginseng and alcohol in a ratio of 1: 5 to 1:20. 8 weeks of primary leach extraction, extract the extract, and then rinse the first extracted ginseng and alcohol water 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container.4 Follow the extraction by extracting the secondary leaching for 3-8 weeks in a cool and dark place to avoid the temperature and sunlight of -10 degrees Celsius, and then leaching the second extracted red ginseng and pH2.5-3.5 strong acidic electrolyte After mixing at the ratio of ˜1: 90, the extract is poured into the 3rd extract by boiling in 4 ~ 6 hours at a temperature of 100 ~ 120 ° C. so that the ratio of 1: 20 ~ 1: 30 is increased, and the extract is followed. After mixing tertiary ginseng extracted with strong acid water bath and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to weight ratio, the high-lysed ginseng is 1: 20-1: 1 at a temperature of 100-120 ° C. 30 ratio Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of temperature 10-40 degrees. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The licorice extract is sealed in a mixed hermetic glass container at a ratio of 1: 5 to 1:20 liquor by weight of licorice and alcohol in a ratio of 1: 5 to 1:20. Extract the liquor, extract the licorice and alcohol, 25% alcohol, then seal it in the mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight. The secondary leaching extraction is carried out for 3-8 weeks in a cold dark place where the temperature and the temperature of -10 degrees Celsius are avoided, and the extracted liquor is followed. After mixing at a ratio of ˜1: 90, the licorice and water are extracted at a temperature of 100 to 120 ° C. in a ratio of 1:20 to 1:30 for 4 to 6 hours, followed by extraction. Licorice extracted from tertiary water bath in strong acid water and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1:60 to 1:90, and the licorice and water were 1:20 to 1: at a temperature of 100 to 120 ° C. 30 ratio Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of temperature 10-40 degrees. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The blueberry extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight of blueberry and alcohol in a ratio of 1: 5 to 1:20. The leachate is extracted for 3-8 weeks at 3-8 weeks, followed by the extraction, and the leached blueberries and alcohol are 25% alcohol in a ratio of 1: 5 to 1:20 by weight and sealed in a mixed sealed glass container. Afterwards, extract the second leaching for 3-8 weeks in a cold dark place where the temperature is 4-10 degrees Celsius and avoid the sunlight, followed by the extract, and weigh the blueberry and pH 2.5-3.5 strongly acidic electrolyzed water extracted by leaching. The mixture is mixed at a ratio of 1:60 to 1:90, and the blueberries and water are extracted at a temperature of 100 to 120 ° C. in a ratio of 1:20 to 1:30 for 4 to 6 hours to be extracted in a third manner. Extract the extract, mix the blueberry extracted with the strong acid water bath in a strong alkali and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to the weight, and then at the temperature of 100-120 ° C. Blueberry and water are poured into the 4th to 6th ratio in a ratio of 1: 20 ~ 1: 30, followed by 4th extract, followed by the extract, and the extract extracted from each of the 4th phase above is mixed and used with a vacuum rotary evaporator. Then, the concentrate and extract are concentrated under 10-40 ° C. under conditions of 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight.

The phosphorus extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight of phosphorus candle and 95% alcohol content, and then stored in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. After extracting the primary leaching for 8 weeks, extract the extract, and seal the primary extracted alcohol and alcohol with 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container. The secondary leaching extract is extracted for 3-8 weeks in a cool and dark place with a temperature of 4-10 degrees Celsius, so that the extract can be followed, and the leached second extract of Indongcho and pH 2.5-3.5 strong acidic electrolyzed water to the weight 1: After mixing at the ratio of 60 ~ 1: 90, the extract and the water are heated for 3 to 4 hours at a temperature of 100 ~ 120 ℃ to be 1: 20 ~ 1: 30, followed by extraction. After mixing tertiary vinegar extracted with a strong acid water bath with pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to weight, the vinegar and water at a temperature of 100-120 ° C Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight to prepare.

The carrot extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight of alcohol and alcohol content of 95% alcohol, and then stored in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. After extracting the primary leaching for 8 weeks, pour out the extract, and seal the primary extract of carrots and alcohol with 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container. Follow the extraction by allowing the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius and avoiding sunlight, and leaching the secondary extracted carrot and pH 2.5-3.5 strong acidic electrolyte water by weight 1:60. After mixing at a ratio of ˜1: 90, the carrots and water are poured into a third of them for 4 to 6 hours at a temperature of 100 to 120 ° C. so as to have a ratio of 1:20 to 1:30, followed by extraction thereof. Carrots were extracted from the carbohydrates in a strong acid water bath and pH10.5-11.5 strongly alkaline electrolyzed water at a ratio of 1: 60-1: 90 to weight, and the carrots were then heated at a temperature of 100-120 ° C. The rate of 30 Pour the extracts in 4th order by boiling them for 4-6 hours, mix the extracts from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of 10-40 ° C. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The tomato extract is sealed in a mixed-sealed glass container in a ratio of 1: 5 to 1:20 of alcohol and alcohol content of 95% alcohol by weight, and then stored in a cool dark place that can avoid the temperature of 4-10 degrees Celsius and sunlight. -After 8 weeks of primary leaching, extract the extracted liquid, seal the first extracted tomato and alcohol 25% alcohol in a ratio of 1: 5 to 1:20 by weight, and seal it in the mixed sealed glass container. Follow the extraction by allowing the secondary leaching to be carried out for 3-8 weeks in a cold dark place where it can avoid the temperature and sunlight of -10 degrees Celsius. After mixing at a ratio of ˜1: 90, the tomato and water are extracted in a third manner by boiling in 4 to 6 hours at a temperature of 100 to 120 ° C. so as to have a ratio of 1:20 to 1:30, followed by the extract. The tomato and water were mixed at a temperature of 100-120 ° C. after mixing the tomato extracted with the strong acid water bath with pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to the weight. Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight to prepare.

The red ginseng extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 red ginseng and alcohol, at a ratio of 1: 5 to 1:20. After extracting the primary leaching for 8 weeks, extract the extract, seal the red ginseng and alcohol with 25% alcohol, which is extracted first, and seal it in the mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight. The secondary leaching extract is extracted for 3-8 weeks in a cool and dark place that can avoid the sunlight and the temperature of -10 degrees Celsius, followed by extracting the extracted red ginseng and pH2.5-3.5 strong acidic electrolyzed water by weight 1:60. After mixing at the ratio of ˜1: 90, the red ginseng fruit is extracted in a third manner by boiling it for 4-6 hours at a temperature of 100-120 ° C. so that the ratio is 1: 20-1: 30, Red ginseng extracted with tertiary water bath in strong acid water and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to weight ratio, and then the red ginseng fruit was 1: 20-1: 30 ratio Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of temperature 10-40 degrees. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The propolis extract is sealed in an airtight glass container at a ratio of 1: 5 to 1:20 by weight of propolis and alcohol in a ratio of 1: 5 to 1:20, and then in a cool dark place that can avoid the temperature and sunlight of 4-10 degrees Celsius. After extracting the primary leaching for 3-8 weeks, extract the extract, and seal the primary extract and 25% alcohol from the primary extract with a ratio of 1: 5 to 1:20 by weight in a sealed glass container. Follow the extraction with a secondary leaching for 3-8 weeks in a cool dark place to avoid 4-10 degrees Celsius temperature and sunlight, and leach the secondary extract of propolis and pH 2.5-3.5 strongly acidic electrolyzed water by weight 1: After mixing at the ratio of 60 ~ 1: 90, propolis and water are boiled for 3 ~ 4 hours in a ratio of 1: 20 ~ 1: 30 at a temperature of 100 ~ 120 ℃, followed by extraction. Propolis was extracted with a mixture of propolis extracted with a strong acidic water bath and pH10.5-11.5 strongly alkaline electrolyzed water at a ratio of 1:60 to 1:90, and the propolis at a temperature of 100 to 120 ° C. Mix the extract with four extracts over 4 to 6 hours and mix the extract from the above, and mix it with the vacuum rotary evaporator. The concentrate and extract are concentrated to 10-40 minutes in a 40-degree seeding condition to obtain a ratio of 1:10 to 1:40 by weight.

The grapefruit seed extract is a cold dark place that can avoid the temperature and sunlight of 4-10 degrees after sealing the grapefruit seed and alcohol content of 95% alcohol in a ratio of 1: 5 to 1:20 in a mixed sealed glass container. The leachate was extracted for 3-8 weeks at 3-8 weeks, followed by extraction, and the 1st extract of grapefruit seed and alcohol was extracted and sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight. Afterwards, extract the second leaching for 3-8 weeks in a cool dark place that can avoid 4-10 degrees Celsius temperature and sunlight, pour out the extract, and weigh the second extracted grapefruit seed and pH 2.5-3.5 strong acidic electrolyzed water. The mixture is mixed at a ratio of 1:60 to 1:90, and the grapefruit seeds and water are mixed in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C for 4 to 6 hours, and extracted in a third manner. After extracting the mixture, the mixture of grapefruit seed extracted with tertiary water bath in strong acidic water and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to weight ratio, and then the grapefruit seed and water at a temperature of 100-120 ° C. 1 4 ~ 6 hours in a ratio of 20 ~ 1: 30 so that it can be extracted in 4th order, follow the extract, mix the extract extracted in each of the 4th from above, and use the vacuum rotary evaporator to make the temperature 10-40 degrees. Concentrate and extract the concentrated solution for 10-40 minutes in the seeding conditions to the ratio of 1:10 ~ 1:40 by weight to prepare.

The rose hip extract is sealed in a mixed hermetically sealed glass container at a ratio of 1: 5 to 1:20 relative to the weight of the rose hips and alcohol by weight of 3% in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. After extracting the primary leaching for 8 weeks, extract the extract, seal the rose hips and the alcohol extracted 25% alcohol in the ratio of 1: 5 ~ 1: 20 by weight, and seal them in the mixed sealed glass container. Allow the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius and sunshine, followed by the extraction and leaching the secondary extracted rosehips and pH2.5-3.5 strong acidic electrolyzed water by weight 1:60 After mixing at a ratio of ˜1: 90, the rose hips and water are extracted in a third manner by boiling in 4 ~ 6 hours so that the rose hips and water have a ratio of 1: 20 ~ 1: 30 at a temperature of 100 ~ 120 ° C. The rose hips and the water, which were extracted with the strong acid water bath 3rd, and the pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to the weight, were mixed at the temperature of 100-120 ° C. Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to prepare a ratio of 1:10 to 1:40 by weight to prepare.

The lavender extract is sealed in a mixed hermetic glass container at a ratio of 1: 5 to 1:20 by weight of lavender and alcohol in a ratio of 1: 5 to 1:20, and then stored in a cool dark place that can avoid temperatures between 4-10 degrees Celsius and sunlight. -After 8 weeks of primary leach extraction, extract the extract, seal it and seal the primary extracted alcohol and 25% alcohol in a ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container.4 The secondary leaching extraction is carried out for 3-8 weeks in a cool and dark place where the temperature is -10 degrees Celsius, and the sunlight can be avoided, followed by the extraction and leaching the secondary extracted lavender and pH 2.5-3.5 strong acidic electrolyzed water by weight 1:60 After mixing at the ratio of ~ 1: 90, the lavender and water are extracted in a third time by boiling in 4-6 hours so that the ratio of the lavender and water is 1: 20-1: 30, and the extract is followed. After mixing tertiary extracted with strong acid water bath and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60 ~ 1: 90 to weight, the lavender and water were heated at a temperature of 100 ~ 120 ℃. Four to six hours of boiling water is added to the ratio of 1:20 to 1:30, followed by four extractions. The extracts are extracted, and the extracts are extracted from each of the four teas from above. Concentrate and extract in concentrated seeding conditions for 10-40 minutes to produce a ratio of 1:10 ~ 1:40 by weight to prepare.

The rosemary extract is sealed in a mixed sealed glass container in a ratio of 1: 5 to 1:20 by weight of rosemary and alcohol in a ratio of 1: 5 to 1:20, and then stored in a cool dark place that can avoid temperatures of 4-10 degrees Celsius and sunlight. -After first extraction for 8 weeks, pour out the extract, seal the rosemary and alcohol with 25% alcohol, which is extracted first, and seal it in the mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight. Follow the extraction by allowing the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius, and leach the secondary rose rosemary and pH2.5-3.5 strong acidic electrolyzed water by weight 1:60. After mixing at a ratio of ˜1: 90, the rosemary and water are extracted in a third manner by boiling in 4-6 hours so that the ratio of rosemary and water is 1: 20-1: 30 at a temperature of 100-120 ° C. The rosemary extracted with tertiary water bath in strong acid water and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1:60 to 1:90 by weight, and then the mixture was heated at a temperature of 100 to 120 ° C. Rosemary and water are mixed for 4 to 6 hours in a ratio of 1:20 to 1:30, followed by 4th extraction. Follow the extract, mix the extracts from each of the 4th from above, and use the vacuum rotary evaporator. It is prepared by concentrating the concentrate and extract in 10-40 minutes at a temperature of 10-40 ° C. in a ratio of 1:10 to 1:40 by weight.

The chamomile extract is sealed in a mixed hermetic glass container at a ratio of 1: 5 to 1:20 by weight of chamomile and alcohol content of 95% alcohol 3, in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius 3 After extracting the primary leaching for 8 weeks, extract the extracted liquid, and seal the primary extracted chamomile and alcohol water 25% alcohol in the ratio of 1: 5 to 1:20 by weight in a mixed sealed glass container. The secondary leaching extraction is carried out for 3-8 weeks in a cold dark place where the temperature and the temperature of -10 degrees Celsius can be avoided, so that the extract can be followed, and the leached chamomile and pH 2.5-3.5 strong acidic electrolyzed water are 1:60 by weight. After mixing at a ratio of ˜1: 90, the chamomile and water are extracted in a third time by boiling in 4 ~ 6 hours so that the ratio of chamomile and water is 1: 20 ~ 1: 30 at a temperature of 100 ~ 120 ° C, The chamomile extracted from tertiary water bath in strong acid water and pH10.5-11.5 strong alkaline electrolyzed water were mixed at a ratio of 1: 60 ~ 1: 90 to weight, and then heated at a temperature of 100 ~ 120 ℃. Chamomile and water in 4 to 6 hours in a ratio of 1: 20 to 1: 30 so that it is extracted into the fourth step to follow the extract, and then mixed with the extract extracted from each of the four above from the above using a vacuum rotary evaporator It is prepared by concentrating the concentrate and extract in 10-40 minutes at a temperature of 10-40 ° C. in a ratio of 1:10 to 1:40 by weight.

The calendula extract is sealed in a mixed hermetic glass container at a ratio of 1: 5 to 1:20 by weight of calendula and alcohol in a ratio of 1: 5 to 1:20, and then stored in a cool dark place that can avoid 4-10 ° C temperature and sunlight. -After 8 weeks of primary leach extraction, extract the extracted liquid, and then seal the calendula and alcohol water extracted by 25% alcohol in the ratio of 1: 5 to 1:20 by weight in a sealed sealed glass container. Allow the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius to avoid sunlight, followed by the extraction and leaching the second extracted calendula and pH2.5-3.5 strong acidic electrolyzed water by weight 1:60 After mixing at a ratio of ˜1: 90, the calendula and water are extracted in a third manner by boiling for 4 to 6 hours at a temperature of 100 to 120 ° C. so that the ratio is 1:20 to 1:30. The calenderula extracted from the strong acid water bath and the alkaline alkaline electrolytic water at pH10.5-11.5 are used at a ratio of 1:60 to 1:90 by weight.

After mixing, the calendula and water are poured into the mixture at 4 to 6 hours in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C, followed by extraction of the extract, followed by extraction from each of the fourth. The extract is mixed and prepared by concentrating the extract and the extract for 10-40 minutes in a ratio of 1:10 to 1:40 by weight using a vacuum rotary evaporator at a temperature of 10-40 degrees.

The rose extract is sealed in a mixed hermetic glass container at a ratio of 1: 5 to 1:20 by weight of rose and alcohol in a ratio of 1: 5 to 1:20. After extracting the primary leaching for 8 weeks, extract the extract, and seal it with 25% of the rose and alcohol, which is extracted first, in a ratio of 1: 5 to 1:20 to the weight of the sealed hermetically sealed glass. Allow the secondary leaching to be extracted for 3-8 weeks in a cool and dark place with a temperature of -10 degrees Celsius to avoid sunlight, pour out the leaching, and extract the secondary extracted rose and pH2.5-3.5 strong acidic electrolyzed water by weight 1:60. After mixing at a ratio of ˜1: 90, the rose and water may be extracted in a third manner by boiling in 4 to 6 hours so that the rose and water have a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C. The rose and water were mixed at a temperature of 100 to 120 ° C. after mixing the rose extracted with tertiary water bath with strong alkaline water with pH10.5-11.5 strongly alkaline electrolyzed water at a ratio of 1:60 to 1:90. The rate of 30 Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of temperature 10-40 degrees. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The nettle extract is sealed in a mixed-sealed glass container at a ratio of 1: 5 to 1:20 by weight of alcohol and 95% alcohol content, and then stored in a cool dark place that can avoid temperatures between 4-10 ° C and sunlight. -After 8 weeks of primary leach extraction, extract the extract, seal the nettle and alcohol content of 25% alcohol extracted by primary leaching in a sealed hermetically sealed glass at a ratio of 1: 5 to 1:20 by weight. The secondary leaching extraction is carried out for 3-8 weeks in a cold dark place where the temperature and the temperature of -10 degrees Celsius can be avoided, so that the extract can be extracted, and the leached second extraction nettle and pH 2.5-3.5 strong acidic electrolyzed water to the weight 1:60 After mixing at the ratio of ~ 1: 90, the nettle and water are extracted in a third time by boiling in 4-6 hours so that the ratio of the nettle and water becomes the ratio of 1: 20 ~ 1: 30 at a temperature of 100-120 ° C. After mixing the nettle extracted with the strong acid water bath and the pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1: 60-1: 90 to the weight, the nettle and water were 1: 20-1 at a temperature of 100-120 ° C. Ratio of: 30 Pour the extracts in 4th order by pouring in 4-6 hours as much as possible, mix the extracts extracted from each of the 4th from above, and use the vacuum rotary evaporator to weigh the concentrates and extracts under the setting conditions of temperature 10-40 degrees. It is prepared by concentrating for 10-40 minutes so that the ratio is 1:10 to 1:40.

The basil extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 by weight of basil and alcohol content of 95% alcohol 3 in a cool dark place to avoid the temperature and sunlight of 4-10 degrees Celsius -8 weeks to extract the first leaching, pour out the extract, leaching the first extraction of basil and alcohol 25% alcohol in a ratio of 1: 5 ~ 1:20 by weight in a sealed sealed glass container 4 Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of -10 degrees Celsius, pour the extract, leaching the secondary extracted basil and pH2.5-3.5 strong acidic electrolyzed water by weight 1: After mixing at a ratio of 60 to 1:90, the mixture of the basil and water at a temperature of 100 to 120 ° C. for 4 to 6 hours in a ratio of 1:20 to 1:30 is extracted in a third manner, followed by extracting the extract. After mixing the third extracted with strong acid water bath and the pH10.5-11.5 strong alkaline electrolyzed water in the ratio of 1: 60 ~ 1: 90 At the temperature of 100-120 ° C., the basil and the water were bathed for 4 to 6 hours in a ratio of 1:20 to 1:30, followed by extraction in 4th order, followed by extracting the extract, and mixing the extract extracted from each of the 4th order from above. And it is prepared by concentrating 10-40 minutes in a concentration of 1:10 ~ 1:40 to the weight ratio of the concentrate and extract in a setting condition of temperature 10-40 ℃ using a vacuum rotary evaporator.

The thyme extract is sealed in a mixed sealed glass container at a ratio of 1: 5 to 1:20 thyme and alcohol content of 95% alcohol, and then 3 -1 week to extract the first leaching, pour out the extract, leaching the first time extracted with alcohol and 25% alcohol content in a ratio of 1: 5 ~ 1:20 by weight in a sealed sealed glass container 4 Let the secondary leaching extract for 3-8 weeks in a cool dark place to avoid the temperature and sunlight of -10 degrees Celsius, pour out the extract, leaching second extraction time and pH2.5-3.5 strong acidic electrolyzed water by weight 1: After mixing in the ratio of 60 ~ 1: 90, the mixture of the above time and water at a temperature of 100 ~ 120 ℃ for 4 ~ 6 hours so that the ratio of 1: 20 ~ 1: 30 is extracted to be extracted in 3rd order , 3 times extracted with strong acid water bath and pH10.5-11.5 strong alkaline electrolyzed water at a ratio of 1:60 to 1:90 At a temperature of 100-120 ° C., the mixture of water and water is extracted for the fourth time in a ratio of 1:20 to 1:30, followed by 4 to 6 hours, followed by extracting the extract, and mixing the extract extracted from each of the above 4 times. And it is prepared by concentrating 10-40 minutes in a concentration of 1:10 ~ 1:40 to the weight ratio of the concentrate and extract in a setting condition of temperature 10-40 ℃ using a vacuum rotary evaporator.

In the above-described configuration, the sealing packaging step (S16) weighs the sap prepared by stirring and weighs the contents and seals the product packaged by sealing it with a molding container made of PP material and a film of PE material, and then sorting through the metal detector. Immediately transfer to the freezer and rapidly freeze the products transferred to the freezer at -18 degrees below the core temperature.

Each of the sap prepared above is taken to measure pH.

The container should be sterilized by air drying after immersion in the disinfectant solution using alcohol, strong acidic electrolyzed water, iodine, etc.

To be stored, the packaged product must be rapidly frozen below the core temperature of minus 18 degrees Celsius or below, and the product must be kept in freezing conditions during distribution.

In the future, the frozen product is thawed and used for 7-8 weeks at room temperature.

Even when frozen sap products are thawed, the anti-wrinkle effect does not change.

Frozen sap products have no freeze denaturing effect even when thawed, so that the viscosity and water holding capacity are kept constant.

In the above series of processes, in order to prevent the growth of microorganisms at room temperature, the process elapsed time should be made within 4 hours during the stirring packaging process and the sealing packaging and the rapid freezing process.

Hereinafter, a method for preparing antioxidant functional sap for wrinkle improvement using squid by-product extract DHA / EPA and natural herb extract prepared according to an embodiment of the present invention, and sap prepared according to the comparative example and other sap prepared according to the comparative example Compare the results of experiments with various characteristics.

Hereinafter, a method of preparing an antioxidant functional moisturizing sap solution for wrinkle improvement using squid by-product extract DHA / EPA and natural herb extract according to an embodiment of the present invention, and the sap manufacturing process prepared according to this process is carried out as follows.

[Pretreatment Process]

1) Collect squid internal organs, which are the by-products of squid removed by squirting on the working table, and remove the foreign substances in the water after screening, remove the foreign substances, weigh 1,000g, immerse in alkaline electrolytic water for 10 minutes, wash and wash in acidic electrolytic water. Sterilize by steeping for 5 minutes.

2) In the electrolysis process, weigh 30.0 g of sodium chloride (20.0 g of tap water) and distilled water, weigh 30.0 g of the electrolytic solution, stir it in the electrolytic cell, and then electrolyze the electrolytic cell in a diaphragm electrolytic cell. An underwater heater, which is a heating facility, is installed in the alkaline electrolytic water collecting tank generated by the cathode to operate the water temperature at 35 degrees Celsius.

In the process of collecting 10,015g strongly alkaline electrolyzed water and 10,015g strongly acidic electrolyzed water generated in the above-mentioned electrolytic water collection process for use as a strong alkaline pretreatment solution and a strong acid pretreatment solution, respectively, the strongly acidic electrolyzed water and the negative electrode produced by electrolysis in the anode side Collect the alkaline alkaline electrolyzed water separately and put it in the sump and circulate to maintain the water temperature at 5-10 degrees Celsius by using chiller which is a cooling facility in each sump.

Underwater ultraviolet sterilizers are installed in each collection container and sterilized so that the pH properties are unchanged. Also, care should be taken to prevent re-contamination of each collected ionized water.

[Intestinal grinding process]

3) Mix the pre-treated squid intestine with 300g of macadamia oil, 300g of jojoba oil, 300g of jojoba gold oil, 300g of hezlot oil, 300g of avocado oil, and 300g of almond oil. Grind to mm size.

Even in this case, disinfect the blade of the shredder or food contact surface around the work table frequently using 70% alcohol.

[Separation Purification Step]

4) Separately purify DHA / EPA with 15% purity by heating 2,800 g of ground guts and oil at 180 ° C for 80 minutes using a molecular path distillation plant.

[Filtration step]

5) Purified DHAEPA with 12-15% purity is qualitatively and quantitatively determined by gas chromatography (GasChromatography) to check purity and filter on activated carbon three times to remove odor.

[Stir Manufacturing Steps]

6) The stirring step is to prepare sap for wrinkle improvement by adding purified and filtered DHA / EPA, natural herb extract, thickener, moisturizer and functional additive using molecular distillation apparatus and chromatography. 3.5g DHA / EPA, 0.5g bokbunja extract, 0.5g Angelica extract, 0.5g round extract, 0.5g Astragalus extract, 0.5g Brown root extract, 0.5g Tobacco extract, 0.5g Oga Extract, 0.5g g mulberry extract, 0.5g mugwort extract, 0.5g ginkgo leaf extract, 0.5g green tea extract, 0.5g red ginseng extract, 0.5g licorice extract, 0.5g blueberry extract, 0.5g honeysuckle extract, 0.5g Carrot Extract, 0.5g Tomato Extract, 0.5g Red Ginseng Extract, 0.5g Propolis Extract, 0.5g Grapefruit Seed Extract, 0.5g Rosehip Extract, 0.5g Lavender Extract, 0.5g Rosemary Extract, 0.5g Chamomile extract, 0.5 g calendula extract, 0.5 g rose extract, 0.5 g nettle extract, 0.5 g basil extract, 0.5 g thyme extract, 0.3 g sodium alginate, 0.3 g carrageenan, 0.3 g pectin, 0.3 g guar gum, 0.3 g xanthan gum, 0.5 g hyaluronic acid, 1.5 g butylene glycol, 1.5 g glycerin, 1.5 g sorbitol, 0. 5 g of placenter, 0.5 g of elastin, 0.5 g of moistin, 0.5 g of lipidus, 0.5 g of natural betaine, 0.5 g of propolis, 0.5 g of ceramide, 3.5 g of tocopherol, 0.5 g of ascor Bic acid, 3.5 g coenzyme Q10, 3.5 g retinol, 0.5 g panthenol, 16.5 g solubilizer, and 47 g purified water are mixed at 70 ° C. under a homogenizer for 15 minutes.

[Sealing packing step]

7) Select each sap prepared above, put it in PP container, seal it with PE material film, seal it, pass it through a metal detector, and transfer to the freezer immediately after sorting.

8) Rapidly freeze the products transferred to the freezer below the core temperature of minus 18 degrees Celsius, and distribute them under freezing conditions.

Hereinafter, a method for preparing antioxidant functional sap for wrinkle improvement using squid by-product extract DHA / EPA and natural herb extract prepared according to an embodiment of the present invention, and sap prepared according to the comparative example and other sap prepared according to the comparative example Compare the results of experiments with various characteristics.

Antioxidant functional sap for wrinkle improvement using squid by-product extraction DHA EPA and natural herb extract composition prepared by the above process [Experimental Example 1] performs a sensory test for skin stickiness and skin pulling according to the thickening effect In [Experimental Example 2], the sensory test was performed on the skin stickiness and skin pulling according to the moisturizing effect. In [Experimental Example 3], the organoleptic and skin pulling according to the content when the thickener and the moisturizing agent were mixed Inspection was carried out.

Experimental Example 1

When adding a certain amount of thickener mixed additives to increase the viscosity, the effect is not added to the moisturizing sap to see the effect (Comparative Example 1) and when the thickener mixed additive is added in a small amount of 0.3% compared to the moisturizing sap ( Comparative Example 2) and the addition of a large amount of 3.0% compared to the moisturizing solution (Comparative Example 3) and 1.5% compared to the moisturizing solution and added each product prepared according to the sap prepared (Example) A sensory evaluayion test was performed on the skin's tactile sensation, which occurred after the skin adhered to the skin and the sap adhered to the skin was completely dried.

Gastric thickener additives include sodium alginate, carrageenan, pectin, xanthan gum, and guar gum as thickeners. In this experiment, each additive was evaluated according to the proportion.

The sensory inspectors were 20 men and women trained in their 20s and 40s, and the score was 5 points (5: very strong, 4: strong, 3: normal, 2: weak, 1: very weak). The results were repeated several times, and the average was shown. The sample was applied by lightly spreading the moisturizing sap product on the skin at 19 ° C. at room temperature for 30 minutes to evaluate the touch felt in each case. The results are shown in Table 1 below.

As a result of the experiment, the skin sticking immediately after application and the skin pulling after drying showed a close correlation. Depending on the amount of the added amount of the moisturizing solution added to the moisturizing sap, the viscosity of the viscosity immediately after application and after 30 minutes was different, but the amount was appropriate as an example when appropriate. This is considered to be close to the value that determines the proper addition ratio since the level of skin stickiness and skin pulling showed a little less than the normal value and slightly exceeded the weak value.

Experimental Example 2

When a certain amount of mixed moisturizer with excellent moisturizing effect is added, the sap is not added to the moisturizing sap to find out the effect of skin moisturizing effect (Comparative Example 4), and when a small amount of the additive is added 6.0% compared to the moisturizing sap ( Comparative Example 5) and the addition of 26.0% large amount of the moisturizing solution (Comparative Example 6) and the addition of 16.0% of the moisturizing solution compared to each product prepared according to the sap prepared (Example) A sensory evaluayion test was performed on the skin's feeling of touch after skin sap and 30 minutes after the application.

The above additives are moisturizers such as hyaluronic acid, butylene glycol, glycerin, sorbitol, placenta, elastin, moistin, lipidur, natural betaine, propolis, ceramide, coenzymecuene, retinol, panthenol. The amount according to the ratio was used and evaluated.

The sensory inspectors were 20 males and females who were trained in their 20s and 40s.The 5-point scoring method (5: very strong, 4: strong, 3: normal, 2: weak, 1: very weak) The results were repeated several times, and the average was shown. The sample was applied to the skin by lightly spreading the moisturizing sap product at room temperature at 19 ° C. for 30 minutes, and the touch of each skin was evaluated. The results are shown in Table 2 below.

As a result of the experiment, the skin sticking immediately after application and the skin pulling after drying showed a close correlation.

According to the amount of moisturizer added to the moisturizing sap, the scores of the viscosity of the moisturizer added after the application and after 30 minutes differed depending on the amount of the moisturizing sap.

This is considered to be close to the value that determines the proper addition ratio because the level of skin stickiness and skin pulling seemed to be slightly lower than the normal value and slightly higher than the weak value.

Experimental Example 3

This experiment is not added to the moisturizing solution (Comparative Example 7) and the additives compared to the moisturizing solution to determine the skin sticking and skin pulling due to the overlapping addition of each additive of [Experimental Example 1] and [Experimental Example 2] Small amounts of 0.3% and 5.0% of each addition were added (Comparative Example 8) and additives were added in large amounts by 3.0% and 15.0% of each moisturizing solution (Comparative Example 9). And 10% of each skin prepared after the sap (Example) prepared according to the duplicated preparation according to the above example, the skin sticking immediately after application and after 30 minutes the skin adhered to the skin after the sap adhered to dry A comprehensive sensory evaluayion test was performed on the pull.

Gastric thickener additives include sodium alginate, carrageenan, pectin, xanthan gum and guar gum as thickeners. In this experiment, each additive was evaluated according to the proportion.

As moisturizer, hyaluronic acid, butylene glycol, glycerin, sorbitol, placenter, elastin, moistine, lipidur, natural betaine, propolis, ceramide, coenzymecuthene, retinol, panthenol. The amount according to the ratio was used and evaluated.

The sensory inspectors were 20 males and females who were trained in their 20s and 40s.The 5-point scoring method (5: very strong, 4: strong, 3: normal, 2: weak, 1: very weak) was used. The samples were repeated and averaged, and the sample was lightly spread on the skin under the condition of 19 ° C at room temperature, and the skin feeling was evaluated for 30 minutes at each time. The results are shown in Table 3 below.

As a result of the above experiment, it was shown that the skin stickiness after spraying and the skin pulling after drying were closely correlated as in the case of [Experiment 1] and [Experiment 2].

The viscosity of the added moisturizer and the amount of the moisturizer added to the moisturizing sap was different depending on the weight of the moisturizing fluid and the amount of the moisturizing agent after 30 minutes.

This is considered to be close to the value that determines the proper addition ratio since the level of skin stickiness and skin pulling seemed to be slightly lower than the normal value and exceeded the weakness value.

Therefore, as shown in the above comparative experiments, when the mass production by the production method of antioxidant functional sap for wrinkle improvement using the squid by-product extraction DHA / EPA and natural herb extract of the present invention can be expected to moisturize the appropriate skin It can be seen that it can provide a moisturizing fluid that induces stickiness and skin pulling.

1 is a flow chart showing a method of preparing an antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA EPA and natural herb extract composition according to an embodiment of the present invention and the sap prepared accordingly.

Claims (12)

A pretreatment step of separating and removing the anal part and then collecting and rinsing the remaining internal organs of the squid body; A viscera crushing step of crushing the pretreated squid viscera with vegetable oil; ( Mixing ratio of vegetable oil and internal organs ?) Separation and refining process of separating and purifying DHA and EPA using boiling point of DHA / EPA itself by heating the ground viscera and oil by using a short path distillation plant at a temperature of 160-200 ° C. for 60-100 minutes. ; A filtration process of filtering the separated DHA / EPA on activated carbon to confirm purity and remove odor by qualitative and quantitative determination using gas chromatography (Gas Chromatography); Stirring manufacturing process for producing a viscous sap by adding the filtered DHA / EPA, natural herb extracts, thickeners and moisturizer with a homogenizer for 10-15 minutes at a temperature condition of 50-70 degrees Celsius; (What is the reference material for mixing the material?) And Sealing packaging by packing the prepared viscous sap in the container and rapidly freezing the sealed product to the core temperature below minus 18 ℃; Antioxidant for wrinkle improvement using squid by-product extraction DHA / EPA and natural herbal extracts comprising Method for producing functional moisturizing sap. According to claim 1, wherein the pre-treatment step is washed with foreign water and dirt, such as squid internal organs in flowing water, the pH 10.5-11.5 alkaline electrolytic water and pH 2.5 of 2-5 times the weight of the internal organs with immersion liquid -3.5 to prepare acidic electrolyzed water, and immersing the washed internal organs in the alkaline electrolyzed water immersion tank for 5-15 minutes, and secondly immersing in the acidic electrolytic water immersion tank for 5-15 minutes. Squid by-product extraction DHA EPA and a method for producing an antioxidant functional moisturizing sap for wrinkle improvement using natural herbal extracts. According to claim 1, wherein the vegetable oil is (1) selected from the group consisting of squid by-product extraction DHA / EPA and a method of producing an antioxidant functional moisturizing sap solution for natural herbal extracts using natural herbal extracts. The method of producing an antioxidant functional moisturizing sap solution for improving wrinkles using squid by-product extraction DHA / EPA and natural herb extracts according to claim 1, wherein the functional additive is further added in the stirring manufacturing process. The method of claim 1, wherein the purified purified DHA / EPA in the separation and purification process of the squid by-product extraction DHA / EPA and the production of antioxidant functional moisturizing sap solution using natural herbal extracts characterized in that the purity of 12-15% Way. According to claim 1, wherein the mixing ratio of the DHA / EPA, natural herb extracts, thickeners and moisturizing agent is 0.1-10% of the total amount of sap by using the extract DHA / EPA and natural herbal extracts Method of producing antioxidant functional moisturizing sap for wrinkle improvement. According to claim 1, The natural herb extract is bokbunja extract, Angelica extract, round extract, Astragalus extract, brown root extract, sesame extract, cucumber extract, mulberry extract, mugwort extract, ginkgo biloba extract, green tea extract, high ginseng extract, licorice extract, Blueberry Extract, Indongcho Extract, Carrot Extract, Tomato Extract, Red Ginseng Extract, Propolis Extract, Grapefruit Seed Extract, Rose Hip Extract, Lavender Extract, Rosemary Extract, Chamomile Extract, Calendula Extract, Rose Extract, Nettle Extract, Basil Extract, Time Extract Method for producing antioxidant functional moisturizing sap solution for wrinkle improvement using squid by-product extraction DHA / EPA and natural herbal extracts, characterized in that the mixture of one or two or more selected from the group consisting of. The method of claim 1, wherein the thickener is selected from the group consisting of sodium alginate, carrageenan, pectin, guar gum, xanthan gum, or a mixture of two or more kinds of squid by-product extract DHA / EPA and natural herbal extracts Method for producing antioxidant functional moisturizing sap for wrinkle improvement. The method of claim 1, wherein the moisturizing agent is one or two or more selected from the group consisting of hyaluronic acid, butylene glycol, glycerin, sorbitol, placenter, elastin, moistin, lipidide, natural betaine, propolis, ceramide Squid by-product extraction DHA / EPA characterized in that the mixture and a method for producing an antioxidant functional moisturizing sap solution for improving wrinkles using natural herbal extracts. The method of claim 4, wherein the functional additive is added 0.1-10.0% to the total weight of the sap, characterized in that one or two or more selected from the group consisting of tocopherol, ascorbic acid, coenzyme Q10, retinol, panthenol Squid By-Product Extraction Method for preparing antioxidant functional moisturizing sap for wrinkle improvement using DHA / EPA and natural herb extract. According to claim 1 and 7, wherein the natural herb extract is 4-10 degrees after sealing each of the extract raw materials and alcohol content 95% alcohol in a ratio of 1: 5 ~ 1:20 by weight in a sealed hermetically sealed glass container To extract the first leaching for 3-8 weeks in a cool dark place to avoid the temperature and sunlight, pour out the extract, leaching the first extracted extract and alcoholic alcohol 25% alcohol 1: 5 ~ 1:20 Sealed in a mixed sealed glass container at a ratio, followed by secondary leaching for 3-8 weeks in a cool dark place where temperature and sunlight of 4-10 degrees can be avoided. .5-3.5 Mix strong acidic electrolyzed water at a ratio of 1:60 to 1:90, then mix the raw material and water in a ratio of 1:20 to 1:30 at a temperature of 100 to 120 ° C for 4 to 6 hours. The extract was extracted three times, followed by decanting the extract, and the raw material and pH10.5-11.5 strong alkali extracted third with a strong acid water bath. After mixing electrolyzed water at a ratio of 1:60 to 1:90 to weight, the extract and water are heated at a temperature of 100 to 120 ° C. for 1 to 20 to 1:30 so as to be extracted for 4 to 6 hours. The extract is poured out, and the extracts are extracted from each of the 4th stages above, and the concentrate and the extracts are 1:10 to 1:40 by weight using a vacuum rotary evaporator at a temperature of 10-40 degrees Celsius. Method of producing an antioxidant functional moisturizing sap solution for wrinkle improvement using DHA / EPA and natural herbal extracts, characterized in that it is prepared by concentrating for 10-40 minutes. Antioxidant functional moisturizing sap for wrinkle improvement using squid by-product extraction DHA / EPA and natural herb extract, which is prepared by the manufacturing method according to any one of claims 1 to 11.

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