KR20120033552A - Cosmetic compositions comprising the extract of acer tegmentosum for enhancing collagen biosynthesis - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Acer tegmentosum extract is provided to promote skin cell proliferation and collagen biosynthesis. CONSTITUTION: A cosmetic composition for promoting skin cell proliferation and collagen biosynthesis contains 0.0001-10 wt% of Acer tegmentosum extract. The extract is isolated from stem bark or whole stem using anhydrous or hydrous low alcohol or acetone, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform, or hexane.

Description

Cosmetic compositions comprising the extract of acer tegmentosum for enhancing collagen Biosynthesis

The present invention relates to a skin external preparation composition containing an extract of Acer tegmentosum, and more particularly, to a cosmetic composition containing an extract of mountain elder, having an excellent effect on promoting skin cell proliferation and collagen biosynthesis.

Skin is the body's primary protective film that protects organs in the body from changes in temperature and humidity, stimuli from the external environment such as ultraviolet rays and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, excessive physical and chemical stimuli and stress malnutrition from the outside degrade the skin's normal function and promote skin aging such as loss of elasticity, keratinization and wrinkle formation, which prevents this phenomenon and makes the skin healthier and more elastic. In order to maintain the conventional use of cosmetics reinforced with bioactive substances obtained from various plants, microorganisms, etc., efforts have been made to effectively maintain skin's inherent functions and to effectively inhibit skin aging by activating skin cells. However, the existing cosmetic raw materials have a number of problems, such as the most ineffective or cause skin side effects. Therefore, research on raw materials having an anti-aging effect without causing skin side effects has been actively conducted.

Mountain beetle is a deciduous broad-leaved arboreous tree of the maple family that grows at the foot of an altitude of 500m or more. The results of the previous studies showed that the extract of M. japonica was inhibited by cancer cell growth (J. Korean Soc. Appl. Biol. Chem. 2006, 49, 322), and antioxidant and lipid peroxidation inhibitors (Korean J. Medicinal Crop Sci. 2007 , 15, 296) and gastritis and peptic ulcer treatment effects are known (Korean Patent Publication No. 2009-0046135). Accordingly, researches on the application of the mountain elm extract to pharmaceuticals or functional foods have been conducted. However, there have been no studies on the application of cosmetics related to skin aging such as skin elasticity reduction, keratinization and wrinkle formation, and specifically related to skin cell proliferation or collagen biosynthesis promotion.

The present inventors have completed the present invention by discovering that the composition for external application for skin containing the extract of wild hawthorn has excellent skin anti-aging effect due to skin cell proliferation and collagen biosynthesis promotion.

Therefore, an object of the present invention is to provide a cosmetic composition excellent in promoting the promotion of skin cell proliferation and collagen biosynthesis by containing the extract of mountain bran as an active ingredient.

According to the present invention to achieve the above object, there is provided a cosmetic composition for promoting skin cell proliferation and collagen biosynthesis containing 0.0001 to 10% by weight of the extract of Acer tegmentosum relative to the total weight of the composition. The mountain elm extract is characterized in that the extract of the stem bark or the entire stem.

The mountain elm extract may include (a) drying and pulverizing a stem bark or whole stem of the mountain elm; (b) the pulverized product of step (a) comprises anhydrous lower alcohol containing 1 to 4 carbon atoms, or methanol, ethanol, acetone, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane Extracting by cooling or warming with at least one solvent selected from the group; And (c) characterized in that obtained by the step of concentrating under reduced pressure the extract of step (b).

Preferably, the extraction step is a group consisting of lower alcohol, acetone, 1,3-butylene glycol, normal propanol, isopropanol and normal butanol containing water, methanol, ethanol and the whole stem bark or stem of the ground mountain bran And after primary extraction with at least one polar solvent selected from, it is reextracted with at least one low polar solvent selected from the group consisting of ethyl acetate, diethyl ether, benzene, chloroform and hexane.

The anti-aging external composition for skin is characterized by having skin elasticity improvement and skin wrinkle improvement effect by promoting skin cell proliferation and collagen biosynthesis.

The cosmetic composition containing the extract of mountain bran of the present invention is excellent in promoting skin cell proliferation and collagen biosynthesis. Due to this effect, it can be usefully used as an external skin preparation for the purpose of anti-aging and wrinkle improvement.

The present invention relates to a cosmetic composition for promoting skin cell proliferation and collagen biosynthesis containing an extract obtained by extracting the stem bark or the entire stem of a mountain bark as an active ingredient.

The mountain elm extract in the present invention comprises the steps of drying and grinding the stem bark or whole stem of the mountain elm; The pulverized product is at least one solvent selected from the group consisting of anhydrous lower alcohol, acetone, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane containing 1 to 4 carbon atoms, anhydrous or methanol and ethanol. Extracting by chilling or warming with cold water; And concentrating the extract under reduced pressure.

More preferably, the mountain elm extract may first be dried and pulverized the stem bark or stem of the mountain bark, and then the lower bark or stem of the ground bark or stem containing the water, methanol, and ethanol. At least one polar solvent selected from the group consisting of 1,3-butylene glycol, normal propanol, isopropanol and normal butanol, followed by at least one selected from the group consisting of ethyl acetate, diethyl ether, benzene, chloroform and hexane It is re-extracted with one low polar solvent. By the extraction method as described above, the content of the active ingredient is maximized and impurities are removed, thereby improving the stability and producing an extract with the maximum efficacy. The extract is used in addition to external skin preparations, such as softening longevity using solubilization technology, nutritional longevity, cream, essence applied to the emulsification technology.

The present invention is described in more detail as follows. Finely pulverize the stem bark or stem of the mountain bark and lower alcohol, acetone, 1,3-butylene glycol, normal propanol, isopropanol, and normal butanol, including water, methanol, and ethanol as extracting solvents for the dry weight of the ground product. Polar solvents such as these or mixed solvents thereof are added 5 to 20 times, followed by immersion extraction and filtration. The filtrate is concentrated under reduced pressure. Water is added to the concentrate to disperse the same, and then a low polar organic solvent such as ethyl acetate, chloroform and diethyl ether is added to the dispersion and shaken. The organic layer is separated and concentrated under reduced pressure to obtain an extract having excellent activity. Extraction method is 12 to 96 hours in the case of cold acupuncture, or anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or diethyl ether as a solvent and left to mature for 3 to 20 days at 4 to 25 ℃ room temperature You can also extract In the case of warm needle, it is different depending on the kind and temperature of the solvent etc. to be used, but it is preferable to perform about 5 to 24 hours at a temperature close to the reflux temperature of the solvent.

Thus produced mountain elm extract has been shown to be useful as a skin anti-aging cosmetics including skin elasticity improvement and wrinkle improvement by showing the effect of promoting the growth and collagen biosynthesis of excellent fibroblasts.

[Example]

Hereinafter, the present invention will be described by the following examples and test examples, but the present invention is not limited by the following examples.

Example 1: Preparation of Mountain Elm Extract

After washing the mountain bark bark with purified water and drying, 100 g of the granulated mountain bark bark powder was added to 1 liter of a 50% aqueous ethanol solution, boiled for 12 hours in an extractor equipped with a cooling condenser and filtered with 300 mesh filter cloth. After aging at ˜15 ° C. for 7 days, it is filtered through Whatman No. 2 filter paper. The filtrate was concentrated under reduced pressure at 70 ° C. using a distillation apparatus equipped with a cooling condenser to distill off the ethanol layer and cool the water layer to room temperature. Put this extract in a 3-liter separatory funnel, add 1 liter of ethyl acetate, shake well, mix well, separate completely into two layers, and take the upper layer (ethyl acetate layer). Transfer the lower layer (water layer) to the separatory funnel again and extract twice more with ethyl acetate as above. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain an extract of 0.7 g of dry weight.

Example 2: Preparation of Mountain Elm Extract

After washing and drying the whole mountain trunks, 100 g of the whole powder of aged mountain trunks was put in 1 liter of 50% aqueous ethanol solution, boiled for 12 hours in an extractor equipped with a cooling condenser, filtered through 300 mesh filter cloth, and filtered through 4 ~ 15. After aging for 7 days at ℃, it is filtered with Whatman No. 2 filter paper. The filtrate was concentrated under reduced pressure at 70 ° C. using a distillation apparatus equipped with a cooling condenser to distill off the ethanol layer and cool the water layer to room temperature. The extract was added to a 3-liter separatory funnel, and 1 liter of ethyl acetate was added thereto, followed by shaking. Transfer the lower layer (water layer) to the separatory funnel again and extract twice more with ethyl acetate as above. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 0.8 g of mountain elm extract.

Example 3: Preparation of Mountain Elm Extract

After washing the mountain bark bark with purified water and drying, 100 g of the granulated mountain bark bark powder was added to 1 liter of a 50% aqueous ethanol solution, boiled for 12 hours in an extractor equipped with a cooling condenser and filtered with 300 mesh filter cloth. After aging at ˜15 ° C. for 7 days, it is filtered through Whatman No. 2 filter paper. The filtrate was concentrated under reduced pressure at 70 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain an extract of 7.3 g of dry weight.

Example 4: Preparation of Mountain Elm Extract

The whole mountain trunk was washed with purified water and dried. Then, 100 g of the whole powder of aged mountain trunk was added to 1 liter of a 50% aqueous ethanol solution, boiled for 12 hours in an extractor equipped with a cooling condenser, and filtered through a 300 mesh filter cloth. After aging at ˜15 ° C. for 7 days, it is filtered through Whatman No. 2 filter paper. The filtrate was concentrated under reduced pressure at 70 ° C. using a distillation apparatus equipped with a cooling condenser and dried to obtain an extract of 8.6 g of dry weight.

Test Example 1 Skin Cell Proliferation Effect Test

In order to confirm the skin cell proliferation effect of the extract of the oleander tree, human normal fibroblasts were inoculated to be 1 × 10 ⁴ cells in each well of a 96-well microplate. Incubate for 24 hours in DMEM (dulbecco, s Modified Eagle's Medium) medium. After incubation, the mountain bark bark extracts of Examples 1 and 3 prepared above and the whole mountain bark stem extracts of Examples 2 and 4 were replaced with DM medium without serum adjusted to a final concentration of 500 µg / ml. Incubate further for 24 hours. 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium (MTT: 3- (4,5-dimdimeth-thiazol-2-yl) -2,5-diphenyl tetrazolium: 5 mg / ml) were added in 10 µl portions, and left for 4 hours, and then the medium portion was discarded. 100 μl of dimethyl sulfoxide solution was added to each well, and the absorbance at 570 nm was measured with a microplate reader.

The procedure was performed to obtain the effect of cell proliferation from Equation 1 below, and the results are shown in Table 1 below.

[Equation 1]

% Cell proliferation = [(absorbance-control absorbance at extract treatment) / absorbance of control] X 100

Sample (500 µg / ml) Absorbance (570nm) Cell proliferation effect (%) Control group 0.320 - Example 1 0.510 59.3% Example 2 0.451 40.1% Example 3 0.376 17.5% Example 4 0.360 12.5%

As confirmed in Table 1, it was found that the extract of Example 1-4 according to the present invention is excellent in normal fibroblast cell proliferation effect. In particular, in the case of Examples 1 and 2, it was confirmed that it shows a more excellent effect.

Test Example 2: Collagen Biosynthesis Promotion Effect Test

In order to investigate the collagen biosynthesis-promoting effect of the extract from the wild hawthorn, DMEM (DMEM) was inoculated with human normal fibroblasts to be 1 × 10 ⁴ cells in each well of a 96-well microplate. : Incubate in dulbecco, Modified Eagle's Medium) for 24 hours. After incubation, the extract of bark stem bark extracts of Examples 1 and 3, and the entire extract of the bark of stems of Examples 2 and 4 were replaced with serum-free DM medium adjusted to a final concentration of 500 µg / ml. Incubate for 48 hours more. 50 μg / ml of ascorbic acid is added to promote collagen synthesis before the last 24 hours of culture. After incubation, each well is washed, replaced with DM medium without serum, and incubated for another 24 hours. After incubation, the supernatant of each well was collected, and the amount of newly synthesized collagen was measured by the amount of PICP using a kit (Kit, Takara, Kyoto, Japan) using procollagen type I-peptide (PICP). PICP amount is shown in Table 2 below to synthesize the collagen in terms of ng / 2X10 ⁴ cells.

Sample (500 µg / ml) Collagen Synthesis Amount (ng / 2X10 ⁴ ) Control group 135 Example 1 200 Example 2 184 Example 3 155 Example 4 150

As confirmed in Table 2, it was found that the extract of Example 1-4 according to the present invention, all of the mountain elm extract was excellent in promoting collagen biosynthesis. Also in this case, the effect of the stem bark extract was better. In addition, it was confirmed that there is a more excellent effect in the case of Examples 1 and 2 with different extraction methods.

Formulation Example 1 Preparation of Milk Crocs

Milk croquettes containing the extract of mountain hawthorn prepared in Example 1 were prepared according to the composition of Table 3 below. As a comparative formulation, a milk lotion containing no mountain elm extract was prepared.

ingredient
Content (% by weight) Formulation Example 1 Comparative formulation example glycerin 8.0 8.0 Butylene glycol 4.0 4.0 Hyaluronic acid extract 5.0 5.0 Beta Glucan 7.0 7.0 Carbomer 0.1 0.1 Mountain elm extract (Example 1) 2.0 - Caprylic Capric Triglycerides 8.0 8.0 Squalane 5.0 5.0 Cetearyl Glucoside 1.5 1.5 Sorbitan stearate 0.4 0.4 Cetearyl Alcohol 1.0 1.0 antiseptic Quantity Quantity incense Quantity Quantity Pigment Quantity Quantity Triethanolamine 0.1 0.1 Purified water Balance Balance

Formulation Example 2: Preparation of Nutritional Cream

Nutritional cream containing the mountain elm extract prepared in Example 1 was prepared according to the composition of Table 4 below.

ingredient Content (% by weight) glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Mountain Bran Extract (Example 1) 1.0 Caprylic Capric Triglycerides 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 antiseptic Quantity incense Quantity Pigment Quantity Triethanolamine 0.1 Purified water Balance

Test Example 3: Skin wrinkle improvement effect

The skin wrinkle improvement effect of the cosmetic composition of Formulation Example 1 and Comparative Formulation Example 1 was measured in 20 healthy women aged 35 to 50 years. First, divided into two groups, Formulation Example 1 to Group A, Comparative Formulation Example 1 to Group B was applied to the face twice a day (morning and evening) for 3 months. Three months later, the extent of wrinkle improvement was evaluated by subject's questionnaire.

The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in Table 5 below.

sample No improvement Minor improvements Moderate improvement A significant improvement Formulation Example 1 One One 4 4 Comparative Formulation Example 1 2 3 4 One

It can be seen from Table 5 that the skin wrinkle improvement effect of Formulation Example 1 containing the mountain elm extract of the present invention is very excellent.

Claims (4)

A cosmetic composition for promoting skin cell proliferation and collagen biosynthesis containing an extract of Acer tegmentosum in an amount of 0.0001 to 10% by weight based on the total weight of the composition. The method of claim 1,
The mountain elm extract is a cosmetic composition for promoting skin cell proliferation and collagen biosynthesis, characterized in that the extract of the stem bark or the whole stem. The method according to claim 1, wherein the mountain elm extract comprises the steps of: (a) drying and grinding the stem bark or entire stem of the mountain elm; (b) the pulverized product is at least one selected from the group consisting of anhydrous lower alcohols containing 1 to 4 carbon atoms or methanol, ethanol, acetone, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane; Extracting by cooling or warming with one solvent; And (c) a cosmetic composition for promoting skin cell proliferation and collagen biosynthesis, which is obtained by the step of concentrating the extract of step (b) under reduced pressure. The method of claim 3, wherein the extracting step comprises lowering alcohol, acetone, 1,3-butylene glycol, normal propanol, isopropanol and normal butanol containing water, methanol, and ethanol from the stem bark or whole stem of the ground mountain bran. Skin first extracted with at least one polar solvent selected from the group consisting of and then reextracted with at least one low polar solvent selected from the group consisting of ethyl acetate, diethyl ether, benzene, chloroform and hexane Cosmetic composition for promoting cell proliferation and collagen biosynthesis.