KR20180001652A - Cosmetic composition for skin barrier comprising extract of Buddleia officinalis or fractions thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for reinforcing skin barrier comprising an extract of Buddleja officinalis or acacetin isolated therefrom as an active ingredient. According to the present invention, the cosmetic composition comprising an extract of Buddleja officinalis or acacetin isolated therefrom as an active ingredient increases expression of genes having a skin barrier reinforcing function, thereby being capable of reinforcing skin barrier.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for enhancing skin barrier comprising extracts of wheat straw or a fraction thereof as an active ingredient,

The present invention relates to a cosmetic composition for enhancing skin barrier containing an extract of wheat seed or a fraction thereof as an active ingredient and more particularly to a cosmetic composition for enhancing skin barrier containing an extract of wheat seed or acacetin isolated therefrom as an active ingredient To a cosmetic composition.

Skin barrier is composed of dead keratinocyte and intercellular lipids, protects skin from external stimuli, and protects skin from evaporation of water. It is a skin barrier that plays a key role in skin health. In other words, it prevents excessive release of water in the body and prevents harmful substances such as chemicals and microorganisms from entering our bodies. It plays an important role in intercellular lipid stability in keratinocyte envelope, which constitutes the surface of dead keratinocytes. The keratinocytes undergo differentiation to form a skin barrier through the keratinization process. Skin barrier function can be aged or destroyed by external factors, and damage to the skin barrier can cause skin moisture loss and wrinkles. The tight junction is a kind of cell junction, composed of occludin, claudin, zona occluden (ZO) and so on. The tight junction can be divided into a part of the cell membrane and an intracellular part. Occludin and claudin are tight junction proteins contained in the cell membrane. This tight junction is responsible for filling the gap between neighboring cells with the intercellular linkage, and also regulating the movement of small molecules. Recently, it has been reported that tight junctions play an important role in skin barrier function. (Aileen S. et al., Journal of Cell Science, 122 (9), 1285, 2009)

Aquaporin is a membrane-penetrating protein that selectively passes through water molecules and is present in all cell membranes, and has a mechanism to increase water absorption of the skin. In mammals, thirteen kinds are known, of which aquaporin 3 is expressed in the keratinocytes of the skin, and absorption and migration of water and glycerol through aquaporin 3 prevent cell water loss and increase skin elasticity. (Ji Li et al., Australasian Journal of Dermatology, 51, 106, 2010)

In the present invention, "Buddleia officinalis" (Buddleia officinalis) is a deciduous shrub belonging to the Majeon family. It has been traditionally used in illnesses in which the liver is not easily visible. The biological effects of the wheat dream include anti-inflammation, Antioxidant (Houghton PJ et al., Journal of Ethnopharmacology, 100, 100-107, 2005). Currently known components of the wheat flour are flavonoids such as linarin, acaciin, and acacetin. (Li J. et al., Zhongguo Zhong Yao Za Zhi, 22 (10), 613, 1997)

However, there has been no report on the improvement of moisturizing effect and skin barrier of wheat extract, acne, acacetin.

As a result, the present inventors have made efforts to overcome the problems of the prior art. As a result, in the case of a cosmetic composition comprising wheat extract or acacetin isolated therefrom as an active ingredient, The skin barrier can be strengthened, and the present invention has been completed.

 Aileen S. et al., Journal of Cell Science, 122 (9), 1285, 2009  Ji Li et al., Australasian Journal of Dermatology, 51, 106, 2010  Houghton P. J. et al., Journal of Ethnopharmacology, 100, 100-107, 2005  Li J. et al., Zhongguo Zhong Yao Za Zhi, 22 (10), 613, 1997

Accordingly, it is a main object of the present invention to provide a cosmetic composition for enhancing skin barrier containing an extract of wheat seed extract or a fraction thereof as an active ingredient capable of enhancing skin barrier by increasing gene expression responsible for skin barrier strengthening function .

According to one aspect of the present invention, there is provided a cosmetic composition for enhancing skin barrier containing an extract of wheatronium or acacetin separated therefrom as an active ingredient.

The term " skin barrier enhancement " of the present invention means strengthening the barrier function of the stratum corneum at the outermost part of the skin. Skin barrier is composed of dead keratinocyte and intercellular lipids, protects skin from external stimuli, and protects skin from evaporation of water. It is a skin barrier that plays a key role in skin health. In addition, keratinocytes make skin barrier through differentiation and keratinization processes. Skin barrier function can be aged or destroyed by external factors, and damage to the skin barrier can cause skin moisture loss and wrinkles. In the present invention, the whey extract or acacetin according to the present invention is a gene which forms a complete stratum corneum through the process of proliferating and differentiating the epidermal layer and the gene of the closely packed structure protein which fills the intercellular space and controls the mass transfer, By increasing the expression of the gene, it enhances the barrier of the stratum corneum, which is located in the outer skin.

In the present invention, the wheat extract may be extracted with any solvent for extracting natural plants, and preferably water, lower alcohols having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or the like And the like.

In the present invention, the acacetin is obtained by fractionating the wheat mononuclearum by a silicone gel chromatography on a wheat-germ extract obtained by extracting with a solvent selected from water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof . In the above chromatography, chloroform, methanol, n-hexane, ethyl acetate or a mixed solvent thereof may be used as the mobile phase, and a mixed solvent of dichloromethane and methanol may be preferably used. The chromatography can be carried out once to several times until the acacetin is purified, and concentration and recrystallization can be carried out if necessary.

In the present invention, the whey extract or acacetin is contained in an amount of 0.001 to 50% by weight based on the weight of the composition.

According to the experimental example of the present invention, acacetin, which is the wheat extract of the present invention or its fraction, has a pilar green which is a gene which forms a complete stratum corneum through proliferation and differentiation of the epidermal layer and transglutaminase -1 and the expression of clodin-1, a close-coupled structural gene that fills the gap between cells and cells and controls mass transfer. In addition, it was confirmed that the expression of aquaporin 3 and pilgar green, which are moisturizing factors, is also increased through artificial skin. As a result, the whey extract or acacetin of the present invention increases the expression of genes that enhance the skin barrier function, thereby increasing the moisture absorption of the outermost layer of the skin and increasing the inter-keratinocyte ligation, Cells can be strengthened and consequently skin barrier function can be improved (see Experimental Example 1-2).

In the present invention, the cosmetic composition according to the present invention may be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, And at least one selected from the group consisting of nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

The ingredients contained in the cosmetic composition of the present invention may contain, in addition to the above-mentioned extracts, the components conventionally used in cosmetic compositions, such as stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.

As described above, the wheat extract of the present invention or a fraction thereof, acacetin, increases the expression of filaggrin, transglutaminase-1, claudin-1 and aquaporin-3, genes that enhance skin barrier function , Skin barrier can be enhanced and improved.

Figure 1 shows the formula of acacetin.
FIG. 2 is a graph showing the expression of the aquaporin 3 gene during the treatment with the wheat extract, acacetin.
FIG. 3 is a graph showing the expression of a pilana green gene in the treatment with wheatronium extract, acacetin.
FIG. 4 is a graph showing the expression of transglutaminase gene 1 in the treatment with wheat extract, acacetin.
FIG. 5 is a graph showing the expression of the Claudin 1 gene when treated with the wheat extract, acacetin.
Figure 6 demonstrates the visual effect of the expression of aquaporin 3 and pilgar green on artificial skin in the treatment of wheat seed extract, acacetin.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1: Preparation of a wheat starch extract

Milonghwa was purchased from Kyungdong market (Seoul, Korea) and dried. After the dried wheat flour was pulverized, 100 ml of 70% ethanol was added to each 10 g, and the mixture was aged at room temperature for 1 day. Then, the extract was sequentially filtered and concentrated under reduced pressure to prepare an extract.

Example  2: Acacetin  Produce

Acacetin was prepared from the wheat extract extracted with n-butanol as a solvent using a chromatographic silica gel column. The fraction solvent conditions are the same as dichloromethane / methanol (30: 1 → 0: 1, v = v). In addition, UV absorption wavelength was compared with acacetin standard and it was confirmed that the above fraction was acacetin.

Experimental Example  One: In keratinocyte cell line  Confirm gene expression

1-1: Human skin Keratinocyte cell line HaCaT  Cell culture

The keratinocyte HaCaT, a constitutive cell line of human skin, was cultured in a DMEM culture medium containing 10% fetal bovine serum (FBS) and antibiotics. The cells were cultured in a 75 ° C flask and a 6-well plate at 37 ° C with 5% CO ₂ . The culture medium was changed every 3 to 4 days. When the cells were overproduced, they were subcultured. HaCaT (5 * 10 ⁵ / well) was added and diluted to 10 ppm with DMEM medium without FBS in each well after 24 hours of incubation and washed with phosphate buffered saline (PBS) To each well. After 24 hours, cDNA was synthesized and PCR was performed to evaluate gene expression level.

1-2: Real-time PCR  Use of moisturizing factor, barrier improvement factor gene expression test

The synthesized cDNA was subjected to real-time PCR (PCR) using a master mix and a SYBR green master mix as a target template and a cyanine dye to finally evaluate gene expression level Respectively. Expression of the gene was compared with the relative value by calibrated quantification of the internal control gene β-actin by the Ct (threshold cycle) method, and the sequence is shown in Table 1 below. (*: P < 0.05, **: p < 0.01 for negative control)

Name of the primer order Aquaporin3 Forward 5'-AGACAGCCCCTTCAGGATTT-3 ' Aquaporin3 Reverse 5'-TCCCTTGCCCTGAATATCTG-3 ' Filaggrin Forward 5'-TAGTGCACTCAGGGGGCTCACA-3 ' Filaggrin Reverse 5'-TCCGGCTTGGCCGTAATGTGT-3 ' Transglutaminase 1 Forward 5'-CCCCAAGAGACTAGCAGTGG-3 ' Transglutaminase 1 Reverse 5'-AGACCAGGCCATTCTTGATG-3 ' Claudein1 Forward 5'-GCTCTAGAATTCCGAGCGAGTCATGGCCAACGC-3 ' Claudein1 Reverse 5'-GCTCTAGAATTCTCACACGTAGTCTTTCCCGCT-3 ' β-actin Forward 5'-GGCCATCTCTTGCTCGAAGT-3 ' β-actin Reverse 5'-GACACCTTCAACACCCCAGC-3 '

As a result, as shown in FIG. 2, it was confirmed that the amount of aquaporin 3 expression was significantly increased by treatment with wheat extract and acacetin 10 ppm. Thus, an increase in aquaporin 3 can enhance the moisturizing function by showing that it can more effectively control intercellular adhesion and water movement through the periplasmic space.

In addition, as shown in FIG. 3, the increase in the pillared green means that the stratum corneum is formed through the process of proliferation and differentiation of the epidermal layer. Thus, in the present invention, acacetin, which is an extract of wheat strawberry, is expected to exhibit an excellent effect on skin moisturization by improving the water retention ability by improving the skin barrier.

Furthermore, as can be seen in FIGS. 4 and 5, the expression of transglutaminase 1 gene, which plays a crucial role in the formation of a corneal envelope, is increased, the internal organ is protected from the external environment, The expression of clathrin-1, which is a construct of tight junctions, Therefore, it can enhance the skin barrier function by showing that it can more effectively perform the adhesion between cells and the control of the migration of water-containing solute and moisture through the periplasmic space.

Experimental Example  2: Histopathological observation of skin

2-1. Artificial skin culture

(RHE: Reconstructed Human Epidermis) in which the degree of moisturizing factor expression by acacetin was reproduced similar to that of human skin. When RHE arrives, it is cultured in growth medium for 24 hours for stabilization and treated with 10 ppm of wheat extract, acacetin.

2-2: Immunostaining Aquaporin 3  And Pillar Green  Expression test

Three days after the culture, the skin tissue was fixed in 10% formalin solution, embedded in paraffin, cut into sections and subjected to tissue immunostaining.

As a result, as shown in FIG. 6, it was confirmed that the whey extract and acacetin-treated aqua purine 3 and fila green were increased in comparison with the control group (-). It was also confirmed that the arrangement of the basal layers was arranged more regularly and the skin layer became thicker. Therefore, it can be confirmed that acne extract, acacetin, is effective for improving skin barrier.

Claims (5)

A cosmetic composition for enhancing skin barrier comprising an extract of wheat straw or acacetin separated therefrom as an active ingredient.
The cosmetic composition for skin barrier strengthening according to claim 1, wherein the whey extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof.
The acacetin according to claim 1, wherein the acacetin is obtained by extracting a wheat mononucleotide with a solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, and isolating the resulting whey extract by silicone gel chromatography Wherein the skin barrier enhances the skin barrier property.
The cosmetic composition for skin barrier strengthening according to claim 1, wherein the whey extract or acacetin is contained in an amount of 0.001 to 50 wt% based on the weight of the composition.
The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a lotion (skin lotion), a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, Wherein the composition has at least one formulation selected from the group consisting of nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

Images