CN110538101A - Application of alcohol extract of buddleja officinalis - Google Patents
Application of alcohol extract of buddleja officinalis Download PDFInfo
- Publication number
- CN110538101A CN110538101A CN201810531651.1A CN201810531651A CN110538101A CN 110538101 A CN110538101 A CN 110538101A CN 201810531651 A CN201810531651 A CN 201810531651A CN 110538101 A CN110538101 A CN 110538101A
- Authority
- CN
- China
- Prior art keywords
- gene
- skin
- alcohol extract
- expression
- buddleja officinalis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 72
- 239000000284 extract Substances 0.000 title claims abstract description 56
- 241000123846 Buddleja officinalis Species 0.000 title claims abstract description 47
- 230000014509 gene expression Effects 0.000 claims abstract description 46
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 41
- 241000628997 Flos Species 0.000 claims abstract description 26
- 239000002537 cosmetic Substances 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 10
- 108010041191 Sirtuin 1 Proteins 0.000 claims description 9
- 230000032683 aging Effects 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 241001113925 Buddleja Species 0.000 claims description 7
- 101150095289 FGF7 gene Proteins 0.000 claims description 7
- 101150061822 HBEGF gene Proteins 0.000 claims description 7
- 238000001704 evaporation Methods 0.000 claims description 7
- 101150099704 Fn1 gene Proteins 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 101150062966 FBN1 gene Proteins 0.000 claims description 5
- 101150090929 NID1 gene Proteins 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000002137 ultrasound extraction Methods 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 4
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- 102100028071 Fibroblast growth factor 7 Human genes 0.000 claims description 2
- 239000000469 ethanolic extract Substances 0.000 claims description 2
- 230000008020 evaporation Effects 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 28
- 210000002469 basement membrane Anatomy 0.000 abstract description 19
- 230000003712 anti-aging effect Effects 0.000 abstract description 15
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract description 14
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract description 13
- 210000002744 extracellular matrix Anatomy 0.000 abstract description 13
- 230000009759 skin aging Effects 0.000 abstract description 10
- 230000001105 regulatory effect Effects 0.000 abstract description 8
- 230000008439 repair process Effects 0.000 abstract description 7
- 230000015572 biosynthetic process Effects 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 4
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 44
- 102100037362 Fibronectin Human genes 0.000 description 9
- 210000004207 dermis Anatomy 0.000 description 9
- 210000004185 liver Anatomy 0.000 description 9
- 230000001737 promoting effect Effects 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 102100031509 Fibrillin-1 Human genes 0.000 description 7
- 210000004177 elastic tissue Anatomy 0.000 description 7
- 108010030229 Fibrillin-1 Proteins 0.000 description 6
- 108010067306 Fibronectins Proteins 0.000 description 6
- 102100031455 NAD-dependent protein deacetylase sirtuin-1 Human genes 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000002615 epidermis Anatomy 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 210000002510 keratinocyte Anatomy 0.000 description 5
- 230000035755 proliferation Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 102000008730 Nestin Human genes 0.000 description 4
- 108010088225 Nestin Proteins 0.000 description 4
- 102100033237 Pro-epidermal growth factor Human genes 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108010014258 Elastin Proteins 0.000 description 3
- 102000003972 Fibroblast growth factor 7 Human genes 0.000 description 3
- 108090000385 Fibroblast growth factor 7 Proteins 0.000 description 3
- 101001027128 Homo sapiens Fibronectin Proteins 0.000 description 3
- 102100033762 Proheparin-binding EGF-like growth factor Human genes 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 210000002808 connective tissue Anatomy 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 210000001339 epidermal cell Anatomy 0.000 description 3
- 229940098448 fibroblast growth factor 7 Drugs 0.000 description 3
- 230000002297 mitogenic effect Effects 0.000 description 3
- 210000005055 nestin Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 210000003462 vein Anatomy 0.000 description 3
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241001164374 Calyx Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 102000004266 Collagen Type IV Human genes 0.000 description 2
- 108010042086 Collagen Type IV Proteins 0.000 description 2
- 241001573881 Corolla Species 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 101800003838 Epidermal growth factor Proteins 0.000 description 2
- 229930186217 Glycolipid Natural products 0.000 description 2
- 101800001649 Heparin-binding EGF-like growth factor Proteins 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 206010023644 Lacrimation increased Diseases 0.000 description 2
- 241001113846 Loganiaceae Species 0.000 description 2
- 208000002720 Malnutrition Diseases 0.000 description 2
- 241000475481 Nebula Species 0.000 description 2
- 206010072170 Skin wound Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 230000024245 cell differentiation Effects 0.000 description 2
- 230000012292 cell migration Effects 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 210000004292 cytoskeleton Anatomy 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 229940116977 epidermal growth factor Drugs 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 108010028309 kalinin Proteins 0.000 description 2
- 230000004317 lacrimation Effects 0.000 description 2
- 230000001071 malnutrition Effects 0.000 description 2
- 235000000824 malnutrition Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 208000015380 nutritional deficiency disease Diseases 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000037394 skin elasticity Effects 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- KDSWDGKIENPKLB-QJDQKFITSA-N verbascoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)CCC=2C=C(O)C(O)=CC=2)[C@@H](CO)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O KDSWDGKIENPKLB-QJDQKFITSA-N 0.000 description 2
- QFRYQWYZSQDFOS-UHFFFAOYSA-N verbascoside Natural products CC1OC(COC2C(O)C(COC3OC(C(O)C(O)C3O)C(=O)O)OC(Oc4cc(O)cc5OC(=CC(=O)c45)c6ccc(O)c(O)c6)C2O)C(O)C(O)C1O QFRYQWYZSQDFOS-UHFFFAOYSA-N 0.000 description 2
- 230000037314 wound repair Effects 0.000 description 2
- 101150033839 4 gene Proteins 0.000 description 1
- 206010000507 Acne infantile Diseases 0.000 description 1
- 241001662132 Balanops australiana Species 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 1
- 206010010726 Conjunctival oedema Diseases 0.000 description 1
- 206010011703 Cyanosis Diseases 0.000 description 1
- 102000001301 EGF receptor Human genes 0.000 description 1
- 108060006698 EGF receptor Proteins 0.000 description 1
- 102100033167 Elastin Human genes 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003964 Histone deacetylase Human genes 0.000 description 1
- 108090000353 Histone deacetylase Proteins 0.000 description 1
- 101000846893 Homo sapiens Fibrillin-1 Proteins 0.000 description 1
- 101000601047 Homo sapiens Nidogen-1 Proteins 0.000 description 1
- 101000871708 Homo sapiens Proheparin-binding EGF-like growth factor Proteins 0.000 description 1
- 240000001899 Murraya exotica Species 0.000 description 1
- 235000008766 Murraya exotica Nutrition 0.000 description 1
- 235000009696 Murraya paniculata Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102100037369 Nidogen-1 Human genes 0.000 description 1
- 241000718543 Ormosia krugii Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000574138 Ozothamnus diosmifolius Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 206010034960 Photophobia Diseases 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
- 108010067787 Proteoglycans Proteins 0.000 description 1
- 102000000477 Sirtuin 2 Human genes 0.000 description 1
- 108010041216 Sirtuin 2 Proteins 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- YMWRMAOPKNYHMZ-LLVTZOIGSA-N [(2r,3r,4r,5r,6r)-6-[2-(3,4-dihydroxyphenyl)ethoxy]-5-hydroxy-4-[(2r,3r,4r,5r,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy-2-[[(2r,3r,4r,5r,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-3-yl] (e)-3-(3,4-dihydroxyphenyl)prop-2-enoate Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@H](O[C@H]2[C@@H]([C@H](O)[C@@H](O)[C@H](C)O2)O)[C@@H](O)[C@H](OCCC=2C=C(O)C(O)=CC=2)O1 YMWRMAOPKNYHMZ-LLVTZOIGSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 208000010217 blepharitis Diseases 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000009400 cancer invasion Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000001608 connective tissue cell Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229940109275 cyclamate Drugs 0.000 description 1
- HCAJEUSONLESMK-UHFFFAOYSA-N cyclohexylsulfamic acid Chemical compound OS(=O)(=O)NC1CCCCC1 HCAJEUSONLESMK-UHFFFAOYSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- LRDGATPGVJTWLJ-UHFFFAOYSA-N luteolin Natural products OC1=CC(O)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 LRDGATPGVJTWLJ-UHFFFAOYSA-N 0.000 description 1
- IQPNAANSBPBGFQ-UHFFFAOYSA-N luteolin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C(O)=C1 IQPNAANSBPBGFQ-UHFFFAOYSA-N 0.000 description 1
- 235000009498 luteolin Nutrition 0.000 description 1
- 210000001365 lymphatic vessel Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 210000001724 microfibril Anatomy 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 108010008217 nidogen Proteins 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035778 pathophysiological process Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- YMWRMAOPKNYHMZ-FFPRBYOHSA-N poliumoside Natural products C[C@@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@H](OCCc3ccc(O)c(O)c3)O[C@H](CO[C@H]4O[C@H](C)[C@@H](O)[C@H](O)[C@@H]4O)[C@H]2OC(=O)C=Cc5ccc(O)c(O)c5)[C@H](O)[C@H](O)[C@H]1O YMWRMAOPKNYHMZ-FFPRBYOHSA-N 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 210000000225 synapse Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
the invention discloses application of a buddleja officinalis alcohol extract, in particular to application of the buddleja officinalis alcohol extract in preparation of an expression up-regulating agent of a skin aging resistant gene. The alcohol extract of flos Buddlejae can promote synthesis of extracellular matrix, stabilize basement membrane structure, stabilize skin structure, induce cell repair-related and anti-aging-related gene expression, and improve skin structure stability and antiaging effect; under a specific concentration range, the expression level of the skin anti-aging related gene can be up-regulated to more than 1.36 times, and can reach 2.13 times at most. The discovery is of great significance for developing skin care products or cosmetics related to anti-aging in the future.
Description
Technical Field
The invention relates to the field of plant extracts and daily chemicals, in particular to application of alcohol extract of buddleja officinalis.
Background
the skin covers the surface of a human body and is in direct contact with the external environment, so that the human body can be protected from being damaged by various external factors, and the human body can be used as a mirror to reflect the health state of the human body. The skin is composed of epidermis, dermis, and subcutaneous tissue, and contains skin appendages, abundant nerves, blood vessels, lymphatic vessels, and muscles. The connective extracellular matrix region of the dermis and epidermis, also known as the Basement Membrane (BM), not only supports the epidermis, but also serves multiple functions, such as the junctions of the dermis and epidermis acting as a selective cellular barrier that generally prevents connective tissue cells from contacting the epidermal cells of the Basement membrane, but allows passage of lymphocytes, macrophages, and neuronal synapses; in addition, many vital phenomena such as cell morphology, cell polarity, cell metabolism, protein distribution in plasma membranes, cell survival, cell proliferation and differentiation, and cell migration are very closely related to the basement membrane. Nestin is an important component of basement membrane and is essential for maintaining the structure and function of basement membrane, which is essential for maintaining normal renewal of skin. The dermis is composed of cells and extracellular matrix, and fibroblasts are main cells of the dermis, and the normal proliferation and differentiation of the fibroblasts maintain the structure of the dermis of the skin and form normal structure and physiological functions; extracellular matrix is a kind of macromolecular substance synthesized and secreted by cells and interacting around the cells, and many life phenomena such as proliferation and differentiation of cells, cell migration and the like are closely related to the extracellular matrix. The extracellular matrix is composed of four major classes of molecules: collagen, elastin, glycoprotein and proteoglycan. The extracellular matrix is finely and skillfully entangled and interwoven through different molecules to form various exquisite three-dimensional structures, so that the skin is endowed with elasticity and tensile strength, and looks plump and full of vitality.
SIRT1(sirtuin 1): one of the famous longevity genes, SIRT1, is a highly conserved protein deacetylase, which participates in regulating longevity caused by energy restriction and is called a longevity gene; the SIRT1 has complex physiological action, participates in regulation of glycolipid metabolism, inflammation, oxidative stress, cell aging, apoptosis and the like, and is closely related to skin aging.
Fibroblast growth factor 7 (FGF 7) gene encodes a Fibroblast growth factor 7 mitogenic and cell survival activity and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The protein is an effective epithelial cell specific growth factor, and the mitogenic activity of the protein is mainly expressed in keratinocytes, so that the proliferation rate of the skin is increased, the renewal capacity of the skin is improved, and the protein has an anti-aging effect.
The heparin binding epidermal growth factor hbegf (heparin binding EGF like growth factor) is a member of the EGF family that binds to and activates EGF receptor growth factors. HBEGF plays a key role in pathophysiological processes, including cancer. After the partial thickness of human skin is damaged, epidermal growth factor is generated to promote cell proliferation and be beneficial to healing of skin wounds, and meanwhile, EGF is the epidermal growth factor to promote the proliferation of epidermal cells and maintain the young state of the skin.
NID1(Nidogen) gene encodes protein nestin 1, which is important protein of basement membrane and plays a role in connecting type iv collagen and laminin 5, and is essential for the structure and function of basement membrane. Whether the structure and function of the basement membrane are normal or not determines whether the skin can be normally renewed and differentiated, the nestin expression is reduced, the basement membrane structure is damaged, the proliferation and differentiation of epidermal cells are influenced, and the skin is aged.
Fibrillin 1 encoded by FBN1(Fibrillin 1) gene is secreted by fibroblasts of dermis into extracellular matrix, and can be used as structural component of calcium-binding microfibril to provide stress structure for elastic and inelastic connective tissues of whole body and mediate a series of cell and matrix interactions. Is an important component of elastic fiber in connective tissue, in the process of forming the elastic tissue, the fibrillin 1 provides a skeleton for storing the elastin, the periphery of the mature elastic fiber grows, the reduction of the skin elasticity is an important characteristic of skin aging, the expression level of the fibrillin 1 is closely related to the skin elasticity, and the reduction of the expression level of the fibrillin 1 is also a key factor causing the skin aging.
Fibronectin, also known as Fibronectin, encoded by the FN1(Fibronectin 1) gene, primarily mediates cell adhesion. Purified fibronectin enhances cell-to-cell adhesion and cell-to-matrix adhesion. By adhesion, fibronectin can regulate the shape of cells and the organization of cytoskeleton through cell signal transduction pathways, promote cell spreading, promote cell growth, and make cell growth inseparable from skin state, and high expression of fibronectin can promote cell growth, so that the skin can be continuously renewed, and can resist aging.
Therefore, the research on how to improve the expression of genes related to key parts and functions of the skin has important significance for improving the stability of the true epidermal structure of the skin and delaying skin aging.
Flos Buddlejae (Buddleja officinalis Maxim.), and name of the Chinese medicinal materials. Also named: the Chinese herbal medicine is prepared from rice dyeing flower, murraya paniculata, broccoli, mongolian butterflybush flower, yellow rice flower, pimple bark tree flower and canton love-pea vine flower, is shrub of buddleia of Loganiaceae and deciduous shrub, and is 1-3 m high. The small branches are gray brown, slightly quadrangular, and densely covered by gray-white villi. The leaf pair is in a shape of a needle, the shape is narrow oval to linear, the length is 5-15 cm, the width is about 3 cm, the tip is tapered, the base is wedge-shaped, the whole edge is provided with small saw teeth, the surface is provided with fine star-shaped hair, the veins are sunken, the lower part is densely provided with grey white to yellow star-shaped hairy hair, and the veins are raised; the petiole is 6-10 mm long and is hairy with grey white; the leaves are withered at the base parts of the two petioles to form a transverse line, the panicle grows at the top of the panicle, grows for 5-12 cm, is densely covered with grey-white soft hair, and the bracts are coated with needles and are covered with villi; calyx is bell-shaped, and the tip is 4 cleft; the corolla is cylindrical, the length is about 1.5 cm, the tip is 4 cracks, the drum is violet, the mouth is orange, and the inside and the outside are soft hair; stamen 4, which grows in the middle of the corolla tube; the ovary is superior, 2 rooms, the tip is hairy, the style is short, and the stigma is not cracked. The capsule is 2-6 mm long, 2 petals are split, and the base part has the reserved calyx and petals. Most seeds are fine and flat. The flowering period is 2-3 months. The fruit period is 7-8 months, is dry flower or flower bud of Buddleja officinalis of Loganiaceae, has effects of dispelling pathogenic wind, cooling blood, moistening liver, and improving eyesight, and can be used for treating conjunctival congestion, swelling and pain, lacrimation, photophobia, cataract, and blepharitis. The 2800 m old shrub cluster or forest edge on sunny hillside, river side, village with elevation of 200 is distributed in China, Plumbum preparatium, Burma, Vietnam, mainly produced in Hubei, Sichuan, Shaanxi, Henan. In addition, it is also produced in Yunnan, Guangxi and Hunan provinces. .
The butterflybush flower was first documented in "Kaibao Bencao" in Song Dynasty, and mentioned in "Bencao Jing Shu" in Ming Dynasty: "Buddleja officinalis, who watches the book of Ben Jing (book of academic records), does not have the symptoms of liver deficiency and heat, covers the liver to open orifices, and eyes can see the blood, and liver blood deficiency indicates the symptoms of cyanosis, nebula, severe liver heat, red swelling, gum secretion, red pulse, infantile bean sore, residual toxicity, and malnutrition. The herb is sweet and effective in tonifying blood, cold in removing heat, and liver blood is sufficient, so that the symptoms are not cured. Mention is made in "materia medica truth of the Qing Dynasty": pale butterflybush flower, sweet and slightly cold … … enters liver meridian to remove heat and nourish nutrient. The liver covers the eyes to induce resuscitation, blood can be seen, skin is bluish and nebula is seen in deficiency, red and swollen lacrimation is seen in heat. The eyes with red veins, the infantile acne and residual toxicity, malnutrition and qi attacking the eyes, so it is sweet in tonifying and cold in removing heat, and liver blood and foot are not healed . However, the flavor is thinner than qi, so it is more powerful with the action of nourishing blood. The flos Buddlejae water extract and its flavonoid monomer components have inhibitory effect on Staphylococcus aureus and beta hemolytic streptococcus, and the verbascoside in flos Buddlejae also has antifungal activity. Luteolin, verbascoside, cyclamate and poliumoside separated from flos Buddlejae have antioxidant activity in vitro. The main effective components and pharmacological research progress of the buddleja officinalis show that the buddleja officinalis has certain research value and application potential in the field of skin external application, including the aspects of improving skin vitality, resisting radiation damage and the like.
Disclosure of Invention
The invention aims to solve the technical problem that substances for improving the anti-aging capacity of skin are lacked in the prior art, and provides application of a buddleja officinalis alcohol extract. The alcohol extract of the buddleja officinalis can improve the expression of genes related to skin aging resistance, and further has the function of delaying the aging of human skin.
the invention firstly provides that the alcohol extract of the buddleja officinalis has the capability of improving the expression of extracellular matrix genes, basement membrane related genes and anti-aging related genes, stabilizes the skin structure, regulates and controls the synthesis of elastic fibers in dermis, and is suitable for preparing the skin external preparation with the effect of delaying aging.
The invention mainly solves the technical problems by the following technical means:
The invention provides application of alcohol extract of buddleja officinalis in preparation of an expression up-regulator of anti-skin-aging genes.
The anti-skin aging gene is not limited to genes directly related to skin aging, such as genes involved in skin tissue damage repair and fibrosis processes, but also relates to genes indirectly related to skin aging, such as basement membrane related genes, the expression of which is improved, so that the stability of a basement membrane structure is promoted, the tight connection of the true epidermis is strengthened, and the skin aging is resisted. The skin aging resistant gene is preferably one or more of SIRT1 gene, FGF7 gene, HBEGF gene, NID1 gene, FBN1 gene and FN1 gene.
The alcohol extract of the buddleja officinalis can be alcohol extract of the buddleja officinalis which is conventional in the field, and preferably alcohol extract of the buddleja officinalis from C1 to C5; more preferably, the buddleja officinalis is an ethanol extract, and the ethanol is preferably 70-95% ethanol;
The alcohol extract of buddleja officinalis is preferably prepared by a method comprising:
(1) Pulverizing flos Buddlejae to obtain powder; the flos Buddlejae is preferably flos Buddlejae whole plant;
(2) Adding ethanol for extraction, and filtering to obtain filtrate; the extraction temperature is preferably room temperature, and the room temperature is 20-30 ℃;
(3) Evaporating the filtrate to obtain; the degree of evaporation is to dryness.
In order to more effectively extract the effective components in the buddleja officinalis: the mesh number of the powder in the step (1) is 20-60 meshes; the ethanol in the step (2) is preferably 70% ethanol, and the addition amount of the ethanol is preferably 8-12 times, more preferably 10 times of the volume of the crushed butterflybush flower; the number of times of extraction in step (2) is preferably 1 or more, more preferably 2; wherein the extraction is preferably ultrasonic extraction, and the frequency of the ultrasonic is preferably 400-550W, more preferably 500W; the ultrasonic treatment time is 25-40min, preferably 30 min.
The filtration method in step (2) is centrifugation, the centrifugation speed is preferably 6000-10000rpm, more preferably 8000rpm, and the centrifugation time is preferably 8-12 minutes, more preferably 10 minutes.
In a preferred embodiment of the present invention, the alcohol extract of buddleja officinalis is prepared by a method comprising:
(1) pulverizing dried whole plant of flos Buddlejae to obtain powder with mesh number of 20;
(2) Adding 70% ethanol with a volume 10 times of the powder, performing 500W ultrasonic extraction at room temperature for 2 times, each for 30min, centrifuging, filtering, and mixing filtrates; the speed of the centrifugation is 8000rpm, and the time of the centrifugation is 10 minutes;
(3) And (5) rotatably evaporating the filtrate to dryness to obtain the product.
The concentration of the alcohol extract of the buddleja officinalis prepared by the method in the expression up-regulator of the anti-skin-aging gene is preferably 0.8-12 mu g/ml; more preferably 1 to 10. mu.g/ml; wherein, the concentration in the expression up-regulator for HBEGF gene is preferably 0.8-1.2 μ g/ml, more preferably 1 μ g/ml; the concentration in the up-regulator for expression of FGF7 and/or FN1 gene is preferably 8 to 12. mu.g/ml, more preferably 10. mu.g/ml.
The expression up-regulator of the anti-skin aging gene is suitable for cosmetics or skin care products.
unless otherwise specified, all references to "extract of Buddleja officinalis" in the present invention refer to "alcohol extract of Buddleja officinalis".
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows:
the alcohol extract of flos Buddlejae can promote synthesis of extracellular matrix, stabilize basement membrane structure, stabilize skin structure, induce cell repair-related and anti-aging-related gene expression, and improve skin structure stability and antiaging effect; under a specific concentration range, the expression level of the skin anti-aging related gene can be up-regulated to more than 1.36 times, and can reach 2.13 times at most. The discovery is of great significance for developing skin care products or cosmetics related to anti-aging in the future.
Drawings
FIG. 1 is a graph showing the results of 1. mu.g/mL of the extract of Buddleja officinalis promoting the expression of FBN1 gene.
FIG. 2 is a graph showing the results of the promotion of FBN1 gene expression by the extract of Buddleja officinalis at 10. mu.g/mL.
FIG. 3 is a graph showing the results of FN1 gene expression promotion by 10. mu.g/mL Buddleja officinalis extract.
FIG. 4 is a graph showing the results of 1. mu.g/mL of a Buddleja officinalis extract promoting SIRT1 gene expression.
FIG. 5 is a graph showing the result of 1. mu.g/mL of the extract of Buddleja officinalis promoting HBEGF gene expression.
FIG. 6 is a graph showing the results of 10. mu.g/mL of a Buddleja officinalis extract promoting SIRT1 gene expression.
FIG. 7 is a graph showing the results of 10. mu.g/mL of the extract of Buddleja officinalis promoting the expression of FGF7 gene.
FIG. 8 is a graph showing the results of 1. mu.g/mL of the extract of Buddleja officinalis promoting the expression of NID1 gene.
FIG. 9 is a graph showing the results of using 10. mu.g/mL of the extract of Buddleja officinalis to promote the expression of NID1 gene.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
example 1 production of alcohol extract of Buddleja officinalis
Pulverizing dried flos Buddlejae 10g to 20 mesh with small medicinal material pulverizer (4. Stand independently) at room temperature, ultrasonically extracting with 10 times volume of 70% ethanol 500W for 2 times (30 min each time) at room temperature, centrifuging (8000rpm, 10min), mixing filtrates, and rotary evaporating to dryness to obtain flos Buddlejae alcohol extract 2.7 g.
Example 2 preparation of skin model
Human primary fibroblasts (NHDF) and human primary keratinocytes (NHEK) were extracted from skin tissues, respectively (extraction method references: Wujing, Zhutangyou, skin tissue engineering [ M ]. Beijing: human military medical Press, 2009: 72-103). Reviving the human primary fibroblast, wherein the reviving process comprises the following steps: taking out the freezing tube from the liquid nitrogen, rapidly putting into water bath at 37 ℃ for melting, and centrifuging at 1000rpm/min by a centrifuge for 10 min. Culturing the cells obtained by resuscitation in a DMEM culture medium at 37 ℃ for one week, digesting (taking the cells out of the culture box, removing the original culture medium, adding PBS15mL to rinse the cells once, adding 8mL of 0.05% trypsin, incubating in a carbon dioxide culture box at 37 ℃ for 8min, taking out, adding 10mL of DMEM culture medium containing FBS in a super clean bench, taking out the cells in a 50mL centrifuge tube with a pipette gun, centrifuging for 10min, removing the supernatant, culturing the cells for later use), mixing with collagen, culturing in a carbon dioxide culture box at 37 ℃ for 3-4 days, digesting and inoculating (5 × 104 cells) the human primary keratinocytes cultured by using a feeder layer to the surface of the dermal model, sequentially performing epidermal immersion culture and gas-liquid phase culture, culturing for 18 days to obtain a 3D skin model for detection (the specific steps for preparing an ACK skin model are detailed in NIANE F. BLE, charbel BOUEZ, al.optimization and Characterization of an Engineered Human Skin equivalent. TISSUE ENGINEERING Volume 11, Number 5/6,2005 Mary Ann Liebert, Inc.).
during the period from the surface culture of the skin model at the stage of inoculating human primary keratinocytes into dermis to the 3D skin model formation days 3 to 18, the blank control (NT) group, the positive control group to which alcohol extract of butterflybush flower (10 μ g/mL) was added, and the positive control group to which alcohol extract of butterbush flower (1 μ g/mL) was added were grouped according to the medium used.
wherein, alcohol extract of flos Buddlejae 10 μ g/mL refers to the amount of alcohol extract of flos Buddlejae in unit volume (mL) culture medium is 10 μ g, about 0.001 wt%.
alcohol extract of flos Buddlejae 1 μ g/mL refers to the amount of alcohol extract of flos Buddlejae obtained in unit volume (mL) of culture medium is 1 μ g, about 0.0001 wt%.
EXAMPLE 3 extraction of RNA
Clipping 3D skin model tissue about 2 x 3mm, and placing into 2mL EP tube; adding 1mL of Trizol, adding 2 steel balls, homogenizing by using a tissue disruptor, and carrying out 50Hz ultrasonic treatment for 5 min; centrifuging at 9000rpm and 4 deg.C for 1min, and transferring the supernatant to a new 1.5mL EP tube; adding 200 μ L chloroform, slightly inverting for several times, mixing, and standing at room temperature for 5 min; centrifuging at 9000rpm and 4 deg.C for 15 min; transferring the upper water phase (about 400 μ L) into a new 1.5mL EP tube, adding isopropanol with the same volume, mixing, and standing at room temperature for 10 min; centrifuging at 9000rpm and 4 deg.C for 10 min; discarding the supernatant, washing the precipitate with pre-cooled 70% anhydrous ethanol for 1 time, and centrifuging at 8000rpm and 4 deg.C for 5 min; after the supernatant is discarded, the precipitate is air-dried for 5-10 minutes and then dissolved in 30 mu L of DEPC (diethyl pyrocarbonate) water; the RNA concentration was determined spectrophotometrically.
Example 4 Gene expression analysis and result determination
RNA purification and quantitative analysis of genes were performed according to QuantiGene R2.0 Plex Assay kit, and gene expression was analyzed using the luminex platform, and the results are shown in FIGS. 1 to 8.
and (4) judging a result:
In the test of determining gene expression, if the expression level of the gene is obviously increased compared with the experimental group without the added extract, the buddleja officinalis can be inferred to promote the synthesis of extracellular matrix and stabilize the structural integrity of basement membrane, stabilize the skin structure, induce the gene expression related to cell repair and anti-aging, and improve the structural stability of the skin and the efficacy of delaying aging. The results are shown in detail in FIGS. 1 to 9.
1. the alcohol extract of flos Buddlejae can improve the expression of extracellular matrix related gene
as can be seen from fig. 1 and 2, the expression of FBN1 gene associated with extracellular matrix was up-regulated by 1.52-fold and 1.83-fold respectively in the positive groups (1 μ g/mL and 10 μ g/mL) with the addition of alcohol extract of buddleja officinalis, and increased by 52% and 83% respectively compared to the blank control, and the gene encoding synthetic fibrillin 1, which is an important component of elastic fiber in connective tissue, provides a framework for storing tropoelastin during the formation of elastic tissue, grows the periphery of mature elastic fiber, and enhances and supports the firmness of the skin tissue structure.
As can be seen from fig. 3, the positive group (10 μ g/mL) to which the alcohol extract of buddleja officinalis was added up-regulated 1.46-fold the expression of FN1 gene associated with extracellular matrix, which is increased by 46% compared to the blank control, and which encodes synthetic fibronectin 1 that mainly mediates cell adhesion, through which fibronectin can regulate the shape of cells and the organization of cytoskeleton via cell signaling pathway, promote cell spreading, and promote the growth of cells.
2. The alcohol extract of flos Buddlejae has effect in improving expression of antiaging related gene
As can be seen from FIGS. 4 and 6, the positive groups (1. mu.g/mL and 10. mu.g/mL) added with alcohol extracts of flos Buddlejae were increased in expression of SIRT1 gene associated with the resistance of cells to external adverse environment and the improvement of repair capacity by 1.65-fold and 1.78-fold, respectively, and increased by 65% and 78% compared with blank control, and the mammalian homolog of the gene encoding the synthesized sirtuin 2 participates in the regulation of glycolipid metabolism, inflammation, oxidative stress, cell senescence and apoptosis, etc., indicating that the extract has the function of improving the cell repair capacity and promoting wound repair.
According to fig. 5, the positive group (1 μ g/mL) added with the alcohol extract of buddleja officinalis was up-regulated by 1.39 times with the expression of HBEGF gene promoting cell proliferation and skin wound healing in the skin model, and was increased by 39% compared with the blank control, which indicates that the extract has the effect of improving cell repair ability and promoting wound repair.
As can be seen from fig. 7, the positive group (10 μ g/mL) to which the alcohol extract of buddleja officinalis was added was up-regulated 1.36-fold in the expression of FGF7 gene associated with anti-aging in the skin model, which was increased by 36% compared to the blank control, and the gene encoded synthetic fibroblast growth factor 7 mitogenic and cell survival activities, which were mainly expressed in keratinocytes, had anti-aging effects.
3. The alcohol extract of flos Buddlejae can improve expression of related genes of basement membrane
As can be seen from fig. 8 and fig. 9, in the positive groups (1 μ g/mL and 10 μ g/mL) to which alcohol extracts of buddleja officinalis were added, the expression of NID1 gene of basement membrane gene was up-regulated by 1.70 times and 2.13 times, respectively, and increased by 70% and 113% compared with the blank control, and the gene encoded synthetic nestin 1, which functions to link type iv collagen and laminin 5, serves to stabilize basement membrane structure, and promotes the integrity and normal renewal of skin structure.
Claims (10)
1. an application of alcohol extract of flos Buddlejae (flos Buddleja officinalis Maxim) in preparing expression up-regulator of anti-skin aging gene is provided.
2. The use of claim 1, wherein the anti-skin aging gene is selected from one or more of the SIRT1 gene, FGF7 gene, HBEGF gene, NID1 gene, FBN1 gene and FN1 gene.
3. the use of claim 1, wherein the alcohol extract of buddleja officinalis is an alcohol extract of buddleja officinalis from the group consisting of C1 to C5; preferably, the buddleja officinalis is an ethanol extract, and the ethanol is preferably 70-95% ethanol;
Preferably, the alcohol extract of buddleja officinalis is prepared by the following method comprising:
(1) Pulverizing flos Buddlejae to obtain powder; the flos Buddlejae is preferably flos Buddlejae whole plant;
(2) adding ethanol for extraction, and filtering to obtain filtrate; the extraction temperature is preferably room temperature, and the room temperature is 20-30 ℃;
(3) Evaporating the filtrate to obtain; the degree of evaporation is to dryness.
4. The use according to claim 3, wherein the powder in step (1) has a mesh size of 20 to 60 mesh.
5. The use of claim 3, wherein said ethanol in step (2) is 70% ethanol, and said ethanol is added in an amount of 8-12 times, preferably 10 times, the volume of said pulverized butterflybush flower;
And/or, the extraction times in the step (2) are more than 1 time, preferably 2 times.
6. The use of claim 3, wherein the extraction in step (2) is ultrasonic extraction, and the frequency of the ultrasonic extraction is preferably 400-550W, more preferably 500W;
And/or the ultrasonic treatment time is 25-40min, preferably 30 min.
7. The use according to claim 3, wherein the filtration method in step (2) is centrifugation, the centrifugation speed is preferably 6000-10000rpm, more preferably 8000rpm, and the centrifugation time is preferably 8-12 minutes, more preferably 10 minutes.
8. the use of claim 3, wherein said alcohol extract of Buddleja officinalis is prepared by a method comprising:
(1) Pulverizing dried whole plant of flos Buddlejae to obtain powder with mesh number of 20;
(2) adding 70% ethanol with a volume 10 times of the powder, performing 500W ultrasonic extraction at room temperature for 2 times, each for 30min, centrifuging, filtering, and mixing filtrates; the speed of the centrifugation is 8000rpm, and the time of the centrifugation is 10 minutes;
(3) And (5) rotatably evaporating the filtrate to dryness to obtain the product.
9. The use of claim 8, wherein the alcohol extract of buddleja officinalis is present in the up-regulator of expression of the anti-skin aging gene at a concentration of 0.8 to 12 μ g/ml; preferably 1 to 10. mu.g/ml; wherein, the concentration in the expression up-regulator for HBEGF gene is preferably 0.8-1.2 μ g/ml, more preferably 1 μ g/ml; the concentration in the up-regulator for expression of FGF7 and/or FN1 gene is preferably 8 to 12. mu.g/ml, more preferably 10. mu.g/ml.
10. The use according to any one of claims 1 to 9, wherein the up-regulator of the expression of the anti-skin-aging gene is used in cosmetics or skin care products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810531651.1A CN110538101A (en) | 2018-05-29 | 2018-05-29 | Application of alcohol extract of buddleja officinalis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810531651.1A CN110538101A (en) | 2018-05-29 | 2018-05-29 | Application of alcohol extract of buddleja officinalis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110538101A true CN110538101A (en) | 2019-12-06 |
Family
ID=68701485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810531651.1A Pending CN110538101A (en) | 2018-05-29 | 2018-05-29 | Application of alcohol extract of buddleja officinalis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110538101A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100028202A (en) * | 2008-09-04 | 2010-03-12 | 닥터후 주식회사 | Composition having skin whitening activity comprising an extract of buddleja officinalis |
| CN106309221A (en) * | 2016-08-31 | 2017-01-11 | 百朗德生物化学(海门)有限公司 | Cosmetic composition capable of removing black eyes |
| CN107308039A (en) * | 2017-06-14 | 2017-11-03 | 佛山市汇汾化妆品科技有限公司 | A kind of anti-aging repairs facial mask |
| CN107362129A (en) * | 2017-08-23 | 2017-11-21 | 广东丸美生物技术股份有限公司 | Skin matrix, preparation method and applications and cosmetics and preparation method thereof |
| KR20180001652A (en) * | 2016-06-24 | 2018-01-05 | 코스맥스 주식회사 | Cosmetic composition for skin barrier comprising extract of Buddleia officinalis or fractions thereof |
-
2018
- 2018-05-29 CN CN201810531651.1A patent/CN110538101A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100028202A (en) * | 2008-09-04 | 2010-03-12 | 닥터후 주식회사 | Composition having skin whitening activity comprising an extract of buddleja officinalis |
| KR20180001652A (en) * | 2016-06-24 | 2018-01-05 | 코스맥스 주식회사 | Cosmetic composition for skin barrier comprising extract of Buddleia officinalis or fractions thereof |
| CN106309221A (en) * | 2016-08-31 | 2017-01-11 | 百朗德生物化学(海门)有限公司 | Cosmetic composition capable of removing black eyes |
| CN107308039A (en) * | 2017-06-14 | 2017-11-03 | 佛山市汇汾化妆品科技有限公司 | A kind of anti-aging repairs facial mask |
| CN107362129A (en) * | 2017-08-23 | 2017-11-21 | 广东丸美生物技术股份有限公司 | Skin matrix, preparation method and applications and cosmetics and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101530490B1 (en) | External use composition containing herb extract for hair growth | |
| CN108815072A (en) | A kind of plant composition fermentation magma and its preparation method and application | |
| CN108042436B (en) | Anti-aging and moisturizing blue copper peptide essence cream | |
| KR102113645B1 (en) | A cosmetic composition for preventing hair loss and promoting hair growth containing spicule powder and natural extract | |
| CN108743501B (en) | Plant fermentation raw stock composition and application thereof | |
| CN114288221A (en) | A method for preparing essence containing polypeptide and plant extractive solution with hair growth promoting effect and its application | |
| KR102606356B1 (en) | Cosmetic composition for regenerating skin and improving skin wrinkle | |
| CN109381390A (en) | A kind of sodium hyaluronate skin lightening Face-protecting mask and preparation method thereof | |
| CN102266373A (en) | Skin externally-used matrix for treating scar and preparation method thereof | |
| US20200009043A1 (en) | Cosmetic Composition for Keloid Scar Improvement | |
| CN108721202B (en) | Roselle stem cell freeze-dried powder eye cream and preparation method thereof | |
| CN112891517A (en) | Cytokine composition for promoting scalp hair follicle regeneration and preparation method and application thereof | |
| CN110652475A (en) | Application of nardostachys chinensis extract | |
| CN110538101A (en) | Application of alcohol extract of buddleja officinalis | |
| CN101375830B (en) | Aloe moisture-keeping nutrient skin-care cream and preparation method thereof | |
| KR101710005B1 (en) | Cosmetic composition comprising cheonjeongpalgindan as active ingredient | |
| CN110652474A (en) | Application of gorse extract | |
| CN109568191A (en) | A kind of hair growth accelerating agent and preparation method thereof | |
| CN113773910A (en) | Artemisia annua essential oil and white tea essential oil for promoting skin cell regeneration and their composition | |
| KR20030055950A (en) | Cosmetic composition containing punica granatum l. extract for anti-aging effect | |
| CN113952281A (en) | Antiallergic composition containing stem cell extract and preparation method thereof | |
| TWI701038B (en) | Orchid bud embryo tissue extract that can activate energy, anti-wrinkle, anti-inflammatory and whitening | |
| CN108113916B (en) | Natural repair peptide mask liquid and preparation method thereof | |
| CN110538100A (en) | application of myrobalan alcohol extract | |
| KR102446610B1 (en) | Cleansing composition having increased scopolin, scopoletin and ginsenosides and its use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191206 |
|
| RJ01 | Rejection of invention patent application after publication |