KR20120009920A - Cosmetic composition for promoting hair growth comprising the extract of walnut - Google Patents

Abstract

PURPOSE: A cosmetic composition containing walnut oil soluble extract or walnut water soluble extract is provided to ensure substantial hair growth effect. CONSTITUTION: A cosmetic composition for promoting hair growth contains extract prepared by adding carbon dioxide to a mixture of walnut shell and walnut fruit. A cosmetic composition for promoting hair growth contains water soluble extract as an active ingredient. The walnut fruit is contained in 0.5-1.5 weight parts based on 1 weight part of walnut shell.

Description

Cosmetic composition for promoting hair growth containing walnut extract

The present invention relates to a cosmetic composition for promoting hair growth, and more particularly, to a hair growth promoting cosmetic composition containing a walnut oil-soluble extract or a walnut water-soluble extract.

Hair growth depends on the action of the hair follicle tissue. The hair follicle tissue is composed of outer root sheath cells, inner root sheath cells, matrix cells and dermal papilla cells. Each cell is not only morphologically specific, but also known to have unique functions. The cells have reaction specificities for various growth factors and hormones.

While other cells of hair follicles are of ectoderm origin, dermal papilla is composed of fibroblast-like cells in the dermis of mesodermal origin, which is located at the lowest end of hair follicle tissue. It is enclosed in epithelial cells.

Hair growth proceeds as the epithelial cells surrounding the dermal papilla divide and form a hair shaft, so the dermal papilla cells are thought to play an important role in regulating the division of the epithelial cells. . In addition, breast papilla is a site where male hormones, which are essential for androgenetic alopecia, act on hair follicles, and are known to play an important role in hair growth / hair loss.

Hair loss has tended to be regarded as a problem only among middle-aged and older men, but in recent years, hair loss of women and young people has increased significantly due to environmental pollution and excessive stress.

Accordingly, the need for management of the scalp and hair is continuously increasing, such as stimulating the menstrual blood of the scalp and promoting blood circulation of the scalp through good eating habits, correct lifestyle, correct hair care, and scalp massage.

On the other hand, walnut is a fruit of walnut tree, a deciduous arborescent of the sputum, and is known to be high in protein and nutritional value, to shine in hair, and to be effective in preventing aging and strengthening kidney function. As is disclosed, the contents described in Patent Publication No. 10-2004-0001683. However, the above contents are insufficient to verify the hair growth efficacy, and there is much doubt about whether there is a substantial hair growth effect.

Therefore, the present invention has an object to develop an extract that can exhibit substantially the ability to promote hair growth using walnuts and to develop it to provide a cosmetic composition.

In order to achieve the above object, the present invention is characterized in that the hair is characterized by containing the extract obtained by supercritical extraction by flowing supercritical carbon dioxide into a mixture composed of walnut shells and walnut crushed in a first form as an active ingredient. Provided is a cosmetic composition for promoting growth.

In addition, the present invention is a supercritical extraction step by flowing supercritical carbon dioxide in the mixture consisting of walnut shells and walnut eggs crushed in a second form; After the extraction, the step of removing the extract and recovering the extraction foil: 20 to 40% butylene glycol aqueous solution of 20 to 40% by weight to 1 part by weight of the extraction foil to the recovered extraction foil, and then 20 Extracting for 20 to 28 hours at 800 ~ 1200Mpa at ~ 24 ℃; provides a cosmetic composition for promoting hair growth, characterized in that it contains a water-soluble extract as an active ingredient.

Hereinafter will be described in detail for solving the problems of the present invention.

The present invention is to produce a cosmetic composition that effectively extracts useful bioactive substances from walnuts to exhibit a substantial hair growth promoting effect.

The cosmetic composition for promoting hair growth of the first aspect of the present invention is obtained by supercritical extraction by pouring supercritical carbon dioxide into a mixture composed of crushed walnut shells and walnut eggs (hereinafter, 'walnut oil' or 'walnut oil extract') ), As an active ingredient.

In addition, the cosmetic composition for promoting hair growth of the second aspect of the present invention comprises the steps of supercritical extraction by flowing supercritical carbon dioxide into a mixture composed of crushed walnut shells and walnut eggs; After the extraction, removing the extract and recovering the extraction foil; And 20 to 40% by weight of the aqueous solution of butylene glycol to the recovered extraction foil by adding 1.8 to 2.8 parts by weight relative to 1 part by weight of the extraction foil, followed by extraction for 20 to 28 hours at 800 ~ 1200Mpa at 20 ~ 24 ℃ It contains a water-soluble extract (hereinafter referred to as 'walnut water-soluble extract') obtained from the step; as an active ingredient.

As a result of measuring the cytotoxicity of the water-soluble walnut extract obtained from the second embodiment of the present invention from the above, it was confirmed that the cytotoxicity is less than that of the general organic solvent extract, the skin safety is superior, and the cell proliferation effect is also higher than that of the general organic solvent extract. It was confirmed that excellent.

In addition, the water-soluble extract of walnut obtained from the present invention showed more than 50% antioxidant activity at 0.5% treatment concentration, showed an excellent anti-inflammatory effect than the sage extract used as a cosmetic material, hair root activity and hair growth effect using C57BL6 mice The hair growth effect was superior to the control group.

     In addition, after preparing a cosmetic composition using the walnut oil and walnut water-soluble extracts obtained from the first and second embodiments of the present invention, and applied to the C57BL6 mouse to measure the hair growth effect, it activates the hair roots and excellent It was confirmed that the hair growth effect.

On the other hand, the mixture of the crushed walnut shell and the walnut egg according to the first or second aspect of the present invention is preferably composed by mixing 0.5 to 1.5 parts by weight of walnut eggs with respect to 1 part by weight of the walnut shell.

In the present invention, by extracting the walnut supercritical extraction or ultra-high pressure can be produced a walnut extract containing a bioactive material useful for promoting hair growth than a conventional organic solvent extraction, as a result of preparing a cosmetic composition, hair roots It was confirmed that the actual hair growth effect is exerted by activation.

1 is a diagram showing the cytotoxicity to the water-soluble extract of walnut of the present invention.
Figure 2 is a diagram showing the cell proliferation effect of the present invention walnut water-soluble extract.
Figure 3 is a diagram showing the antioxidant effect of the present invention water-soluble walnut extract.
Figure 4 is a view showing the anti-inflammatory effect of the present invention walnut water-soluble extract.
Figure 5 is a diagram showing the hair growth effect of the present invention walnut oil soluble extract (walnut oil) and walnut water-soluble extract.
Figure 6 is a diagram showing the hair follicle activation effect of the present invention walnut oil-soluble extract (walnut oil) and walnut water-soluble extract.
Figure 7 is a view of the hair growth state after the hair growth promoting cosmetic composition (Example 3) containing a water-soluble extract of walnut of the present invention to the mouse skin.
8 is a diagram illustrating the state of hair follicle tissue after treating the skin of rats with a cosmetic composition (Example 3) for promoting hair growth containing a walnut water-soluble extract of the present invention.

Hereinafter, the content of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

Example  1: Walnut Soluble Extract Preparation

Walnut shell and walnut egg (1: 1 ratio, total 400g) and then walnut supercritical extract by extracting for 3 hours while injecting CO ₂ at a rate of 40bar / hr using a supercritical extractor (SCF, Korea.) Obtained

The extraction yield was 13.20% (55.80g) of the first walnut amount, the extracted walnut oil extract (hereinafter referred to as 'walnut oil') was used in the experiment to remove impurities using a 400 mesh filter.

Example  2: Walnut Water Soluble Extract Manufacturer

Extract the walnut oil in Example 1 and mixed with 300g of the remaining extraction foil 300g 30% butylene glycol aqueous solution 700g using an ultra-high pressure extractor (TSF-10, Japan) at a temperature of 22 ℃, 1000mPa 24 Extraction over time. Thereafter, the extract was recovered, first filtered with 400 mesh, and then sterilized to obtain an extract of 750 g (yield 75%). At this time, the remaining amount of the dried extract was 0.2%, the sugar concentration was 30 brix °.

Experimental Example  1: Determination of Cytotoxicity of Water-Soluble Extracts from Walnuts in in vitro )

The cells were treated with fibroblasts constituting the dermal dermal layer, and cell viability was evaluated by measuring MTT assay after 24 hours.

As a result of the measurement (FIG. 1), it was confirmed that the water-soluble extract of walnut obtained by ultra high pressure extraction has less cytotoxicity than the general organic solvent extract (70% ethanol extract) and has excellent safety on skin.

Experimental Example  2: Measurement of Cell Proliferation Effect of Fibrous Cells of Walnut Water-Soluble Extracts in in vitro )

Samples were treated to fibroblasts constituting the dermal dermal layer, and 48 hours later, MTT assay was used to measure cell proliferation effects.

As a result of the measurement (FIG. 2), it was confirmed that the water-soluble extract of walnut obtained by ultra high pressure extraction showed about 25-30% higher cell growth promoting effect than the organic solvent extract (70% ethanol extract).

From the above results, it can be inferred that when the ultra-high pressure extraction technique of the present invention is used, physiologically active substances useful for skin can be effectively extracted from walnuts.

Experimental Example  3: Determination of Antioxidant Effect of Water-Soluble Extracts from Walnuts in in vitro )

The antioxidant effect of walnut soluble extract was measured by DPPH method.

As a result of the measurement (FIG. 3), the treatment concentration of the water-soluble extract of walnut showed an antioxidant activity of 50% or more. From this, it could be inferred that it would be effective in inhibiting cellular senescence.

Experimental Example  4: Determination of anti-inflammatory effect of walnut soluble extract ( in in vitro )

The IL-1a inhibitory effect of walnut soluble extract was measured by ELISA.

As a result of the measurement (FIG. 4), it was confirmed that about 20% was suppressed at 1.6% of the treatment concentration, and exhibited a superior IL-1a inhibitory effect than the sage extract, which is an anti-inflammatory material of cosmetics.

Experimental Example  5: Hair Growth of Walnut Oil and Walnut Water Soluble Extract Promotion  And changes in hair follicle tissue

Veterinary quarantine is carried out after the animal is obtained, and only 7 healthy animals with no acclimation period have been detected. Only at the end of acclimatization, the electric clipper (ER143. Panasonic. Japan) was used. First hair removal.

Then, shaving form (Gilet foamy sensitive. Gillette (35735378). Secondary hair removal was performed on the width of the dorsal midline up to 1 cm or more from the left and right of the dorsal midline, and only the individuals with homogeneous pink color on the dorsal skin were selected and used in the experiment.

Group separation was performed the next day of hair removal, and was separated by Z-array method on the basis of weight using only animals that did not cause abrasion and severe redness during hair removal.

The individual identification of the animals used in the test was to use the tail marking method (tail marking), and each breeding box was attached with a label containing the test number, test group, and animal number.

In order to administer the sample, after applying a brush to the epilation site, 200 μl of the test substance is dispensed to the tip of the brush using a micropipette, and then uniformly applied to the entire epilation site once a day for 21 days in the vertical direction. (G1: negative control-buffer, G2: positive control-3% minoxidil, G3: 5% walnut oil, G4: 5% walnut aqueous extract).

Body weight was measured on the 2nd and 7th days of the acclimation period, group separation was performed using the 7th day weight data, and body weight was measured once a week during the test period. Body weight measurement was performed at a certain time before application of the test substance.

After the start of the experiment, a daily amount of feed and drinking water was supplied between a certain time, and the remaining amount was measured at the same time the next day and divided by the number of animals per cage (cage) to calculate the daily food and drinking intake per subject.

All test results were expressed as 'mean ± standard deviation', 'Turkey test' was performed to compare the homogeneity of variance for all data, and if the dispersion was homogeneous, one-way analysis of variance (ANOVA) was performed. When significance was observed, 'Dunnett's t-test' was performed to find a test group with significant differences from the control group.

 Mice were autopsied at 3 rats at the end of 10 days after the start of the test and 3 rats at the end of the test (after 21 days), and the sample application site was extracted using an anatomical apparatus and fixed in formalin. Step by step dehydration with alcohol (alcohol) and xylene (xylene) and then embedded in paraffin, using a tissue slicer to make a 5㎛ section to remove the paraffin with alcohol (alcohol) and xylene (xylene) again.

Thereafter, H & E (Hematoxylin & Eosin) staining was used to observe the histological changes of the hair follicle tissue by light microscopy.

(1) hair growth state change measurement

  After depilation, I took a photo using a digital camera (Canon EOS 30D.Canon. Japan) four times a day, 7, 10, 17, 21, and was designated to minimize the effect of lighting in the animal room (SPF-238). The photographs were taken at 200 ~ 300 lux real-time photo table (Copy stand CS-30.LPL. Japan).

The distance to the subject was the same as 70cm for each photographing condition.The lens size (Canon zoom lens, EF 28-105mm 1: 3.5-4.5Ⅱ) and the mode condition were auto mode (AI focus on) , The center subject focus, auto white balance, flash), was taken as a digital image (digital image) of 1504x1000, and saved as a jpg file (file).

Photographs were taken before the test material application on the day. Animals used for photography were lightly anesthetized with dimethyl ether.

As a result of the measurement (FIG. 5), it was confirmed that the hair growth state of the positive control group, 5% walnut water-soluble extract and 5% walnut oil applied test group was superior to the negative control group. After 10 days of application, hair growth was insufficient in the negative control group, but the test group coated with walnut oil and walnut water-soluble extract showed excellent hair growth effects. Especially, the test group coated with walnut oil after 21 days showed the entire hair. I could confirm this.

(2) measuring changes in hair follicle tissue

On the 10th and 21st day of the test, the experimental animals were dislocated and killed by cervical spine in each group, and the tissue of the first epilation site was extracted. The cut tissues were fixed for 1 to 2 days in 10% neutral formalin unfolded using filter paper (Watman No. 2 185 mm, Watman, Germany). Four sections of skin tissue 0.5 cm wide and 2.5 cm long were obtained for each individual. Four skin tissues were paired and fabricated into paraffin blocks after general tissue treatment. The H & E stain was performed by cutting into H. Tissue sections were examined for histological changes in hair follicle tissue such as skin thickness, hair follicle number, hair follicle size, and depth under an optical microscope (JP / BX51. Olympus. Japan).

As a result of the measurement (FIG. 6), it was confirmed that the hair root cells (black) were elongated in the walnut oil and the walnut water-soluble extract. In particular, the hair root cells were excellent in the growth of the walnut oil-treated group.

From the above results, it can be inferred that walnut oil and walnut soluble extract have a hair growth effect through promoting hair root activity.

Example  3: hair Tonic solution  Produce

Using a walnut water-soluble extract and walnut oil-soluble extract was prepared a hair tonic liquid as shown in Table 1.

Raw material name weight% L-menthol 0.30 Salicylic acid 0.25 Di-panthenol 0.20 Walnut Water Soluble Extract 5.00 Walnut Soluble Extract 5.00 Disodium ID 0.05 Methylparaben 0.10 Fiji-60 Hydrogenated Castor Oil 0.50 Propylene glycol 5.00 ethanol 25.00 Herbal extract 15.00 Triethanolamine 0.2 Purified water Balance Sum 100

Experimental Example  6: Measurement of wool area and histological changes

The wool effect of the hair tonic solution prepared in Example 3 was measured (see Experimental Example 5 Method).

(1) wool area measurement

Photographs were taken using a digital camera (Canon EOS 30D. Canon. Japan) four times a day, 7, 10, 17, and 21 days after depilation according to the method described in Experimental Example 5.

  Using the Image Analysis Software (Image Partner. SaramSoft. Korea) for each image evaluation point, the percentage of hair growth in the total area of hair removal was calculated as a percentage (%) of the image file obtained in the photo evaluation. .

The degree of hair growth was analyzed using Image J on the four photographs taken on 7, 10, 17, and 21 days on the day of depilation of the experimental animals (see Table 2).

Hair growth Scoring Judgment 0-20% - no effect 21-40% ± weak 41-60% + Good 61-80% ++ Excellent Over 81% +++ Very good

 As a result of the analysis (FIG. 7), the control group coated with physiological saline only showed hair follicle growth at an average area of 38.63 ± 18.79% at 7 days and an average of 73.27 ± 40.83% area at 21 days.

Minoxidil, a positive control, showed hair follicle growth at an average of 69.64 ± 14.98% on day 7 and 90.59 ± 3.40% on day 17.

In Example 3, hair follicle growth was observed at an average area of 37.83 ± 18.80% on day 7, and an average 82.5 ± 3.71% area on day 21 .

 As a result of comparing the growth of hair follicles on the basis of the wool area, it was confirmed that the hair tonic solution of Example 3 showed a faster hair growth effect than the control group coated with physiological saline only.

(2) Histological Change Measurement

To examine the morphological changes of hair follicles in the control and test groups, skin tissues collected on the 10th and 21st day of the test were stained with 'hematoxylin-eosin' and observed under an optical microscope.

As a result of the measurement (FIG. 8), in the control group, a large number of hair follicles were formed around 10 days after hair removal, but the hair follicles did not grow into the epithelial tissue layer.

On the other hand, the minoxidil 3% application group had a thicker skin, increased number of hair follicles, and increased hair follicle tissue length than the control group on the 10th day.

In Example 3, the thickness of the skin was increased, the number of hair follicles increased, and the length of the hair follicle tissue was increased as compared to the control group, as in the minoxidil 3% applied group.

Claims (3)

A cosmetic composition for promoting hair growth, comprising extracting the supercritical carbon by pouring supercritical carbon dioxide into a mixture composed of crushed walnut shells and walnut eggs as an active ingredient.
Supercritical extraction by pouring supercritical carbon dioxide into the mixture composed of crushed walnut shells and walnut eggs;
After the extraction, removing the extract and recovering the extraction foil; And
After the addition of 1.8 to 2.8 parts by weight of 20 to 40% butylene glycol aqueous solution compared to 1 part by weight of the extracted foil, the extraction step for 20 to 28 hours at 800 ~ 1200Mpa at 20 ~ 24 ℃ Hair growth promoting cosmetic composition comprising a water-soluble extract obtained as an active ingredient.
The method according to claim 1 or 2,
The mixture is
Hair growth promoting cosmetic composition, characterized in that 0.5 to 1.5 parts by weight of walnut shells are mixed with 1 part by weight of walnut shells.

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