JP6655296B2 - Hair growth or hair growth promoter - Google Patents

Description

  The present invention relates to an extract having a hair growth promoting activity and a hair growth promoting activity obtained from a plant. More specifically, forsythia (Forsythia), Obahimemeo (Pouzolzia), Sagaribana (Barringtonia), Anogenesisus (Anogeissus), Aiwatushi (Ailanthus) and at least belongs to the genus selected from the group consisting of the genus Komatsunagi (Indigofera). The present invention relates to a hair growth or hair growth promoting agent containing an extract derived from one plant as an active ingredient.

  Hair repeatedly grows and falls according to a periodic hair cycle consisting of a anagen, a catagen and a telogen. In the hair cycle, the stage in which a new hair follicle is formed from the telogen to the anagen is considered to be the most important for hair growth. In this stage, the growth and differentiation of hair follicle epithelial cells are considered. It is thought that the dermal papilla cells play an important role. Hair papilla cells are cells located inside the root of the hair follicle wrapped in the basement membrane, inside the hair follicle epithelial cells composed of outer root sheath cells and matrix cells near the hair root. It plays an important role in the growth and differentiation of hair follicle epithelial cells and in the formation of hair, such as by acting on epithelial cells to promote their proliferation.

  A condition in which the hair cycle is stagnant or shortened is referred to as alopecia. BACKGROUND ART Hair loss caused by diseases such as abnormalities and drugs such as anticancer drugs is known. Since hair loss greatly affects the appearance of a patient, development of a fundamental treatment or prevention method is desired.

  Finasteride or minoxidil is commercially available as a drug used for treatment or prevention of alopecia, but it is known that finasteride causes sexual dysfunction and minoxidil causes itching and inflammation.

  On the other hand, plant extracts that have been used for a long time in folk medicine and traditional medicine (for example, Chinese medicine) have the advantage that they have a low risk of side effects and can be taken or taken safely for a long period of time. I have. Therefore, in the field of pharmaceuticals and foodstuffs, active research on plant-derived active ingredients has been actively conducted in Japan and overseas, and plant extracts that can be used for the prevention or treatment of alopecia are also subject to search. Has become. For example, it has been reported that an extract of mulberry bark, which is known to have an antitussive effect, a diuretic effect, and the like, has hair-growth activity (Patent Document 1).

  Under such circumstances, a hair growth or hair growth promoting agent that is more effective and has no side effects is desired.

JP 2014-218479 A

  An object of the present invention is to provide a hair growth or hair growth promoter containing a plant-derived extract as an active ingredient.

  The present inventors have found that extracts derived from plants belonging to the genus Forsythia (Forsythia), the genus Obahimemao (Pouzolzia), the genus Sagaribana (Barringtonia), the genus Anogenissus (Anogeissus), the genus Ailanthus, the genus Komatsunagi (Indigofera), For the first time, they have found that they have the effect of shortening the transition period from the telogen to the growth phase of the cycle and the effect of increasing the elongation rate of hair, and have completed a hair growth or hair growth promoting agent containing the extract.

That is, the present invention
[1] At least one member belonging to a genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus, and Indigofera. A hair growth or hair growth promoting agent, comprising an extract from two plants;
[2] The hair growth or hair growth promoting agent according to [1], wherein the extract derived from a plant belonging to the genus Forsythia is an extract derived from above-ground parts;
[3] The hair growth or hair growth promoting agent according to [1] or [2], wherein the plant belonging to the genus Forsythia is Forsythia japonica Makino;
[4] The hair growth or hair growth promoting agent according to [1], wherein the extract derived from a plant belonging to the genus Pouzolzia is an extract derived from above-ground parts;
[5] The hair growth or hair growth-promoting agent according to [1] or [4], wherein the plant belonging to the genus Pouzolzia is Pouzolzia pentandra (Roxb.) Benn.
[6] The hair growth or hair growth promoting agent according to [1], wherein the extract derived from a plant belonging to the genus Barringtonia is an extract derived from bark;
[7] The hair growth or hair growth promoting agent according to [1] or [6], wherein the plant belonging to the genus Barringtonia is Barringtonia pedicellata Ridl.
[8] The hair growth or hair growth-promoting agent according to [1], wherein the extract derived from a plant belonging to the genus Anogeissus is an extract derived from bark;
[9] The hair growth or hair growth promoting agent according to [1] or [8], wherein the plant belonging to the genus Anogeissus is Anogeissus acuminata Wall.
[10] The hair growth or hair growth promoting agent according to [1], wherein the extract derived from a plant belonging to the genus Ailanthus is an extract derived from a branch;
[11] The hair growth or hair growth promoting agent according to [1] or [10], wherein the plant belonging to the genus Ailanthus is Alaanthus triphysa (Dennst.) Alston;
[12] The hair growth or hair growth promoting agent according to [1], wherein the extract derived from a plant belonging to the genus Indigofera is an extract derived from a whole plant;
[13] The hair growth or hair growth promoting agent according to [1] or [12], wherein the plant belonging to the genus Komatsunagi (Indigofera) is Komatsunagi (Indigofera pseudotinctoria Matsum.);
[14] The agent for promoting hair growth or hair growth according to any one of [1] to [13], wherein the extract is an extract extracted with an organic solvent;
[15] The hair growth or hair growth promoting agent according to [14], wherein the organic solvent is 100% methanol;
I will provide a.

  Forsythia, Forsythia, Pouzolzia, Sagaribana (Barringtonia), Anogeissus (Anogeissus), Aluanthus (Ailanthus) and at least one plant belonging to the genus selected from the group consisting of the group consisting of Indigofera Can be provided as a hair growth or hair growth promoting agent.

It is a graph which shows the average value of the score of each observation day in each test group.

  The present invention, at least belonging to the genus selected from the group consisting of Forsythia (Forsythia), Obahimemeo (Pouzolzia), Sagaribana (Barringtonia), Anogenesisus (Anogeissus), Aiwatushi (Ailanthus) and Komatsunagi (Indigofera). An agent for promoting hair growth or hair growth comprising an extract derived from one plant.

  In the present specification, the plant belonging to the Forsythia genus (Forsythia) is not particularly limited as long as it is a plant belonging to the Forsythia genus (Forsythia), and, for example, a mountain forsythia (Forsythia japonica Makino), a forsythia (Forsythia suspensa (Thunb.)) Vahl), Chinese forsythia (Forsythia viridissima Lindl.), Korean daffodils (Forsythia viridissima Lindl. Var. Koreana Rehder), and the like, and preferably Japanese yam forsythia (Forsythia japonica Makino).

  The extract derived from a plant belonging to the genus Forsythia may be an extract derived from the whole plant or an extract derived from a part thereof. Portions include aerial parts (eg, trunks, branches, bark, leaves, flowers, fruits and seeds) or underground parts (eg, roots). The extract derived from a plant belonging to the genus Forsythia of the present invention is preferably derived from the above-ground part, and it is preferable to use the whole above-ground part for a young tree, or to use branches and leaves for an adult tree.

  In the present specification, the plant belonging to the genus Oobahimemao (Pouzolzia) is not particularly limited as long as it is a plant belonging to the genus Oobahimemao (Pouzolzia), for example, Otogirimao (Pouzolzia pentandra (Roxb.) Benn.), Oobahimemao (Pouzolzia zeylanica) (L.) Benn.) And the like, and preferably, Potosilimao (Pouzolzia pentandra (Roxb.) Benn.).

  The extract derived from a plant belonging to the genus Pouzolzia may be an extract derived from the whole plant or an extract derived from a part thereof. Portions include an aerial part (eg, trunk, branches, bark, leaves, flowers, fruits and seeds) or an underground part (eg, root). The extract derived from a plant belonging to the genus Pouzolzia of the present invention is preferably derived from the above-ground part, and it is preferable to use the whole above-ground part for a young tree, and to use branches and leaves for an adult tree.

  In the present specification, a plant belonging to the genus Sagaribana (Barringtonia) is not particularly limited as long as it is a plant belonging to the genus Sagaribana (Barringtonia). .) Kurz) and the like, preferably Barringtonia pedicellata Ridl.

  The extract derived from a plant belonging to the genus Barringtonia may be an extract derived from the whole plant or an extract derived from a part thereof. Portions include aerial parts (eg, trunks, branches, bark, leaves, flowers, fruits and seeds) or underground parts (eg, roots). The extract derived from a plant belonging to the genus Barringtonia of the present invention is preferably derived from above-ground parts, and more preferably derived from bark.

  In the present specification, the plant belonging to the genus Anogenesis (Anogeissus) is not particularly limited as long as it is a plant belonging to the genus Anogenissus (Anogeissus), for example, Yong (Anogeissus acuminata Wall.), Gatinoki (Anogeissus latifolia) and the like. And preferably Yong (Anogeissus acuminata Wall.).

  The extract derived from a plant belonging to the genus Anogeissus may be an extract derived from the whole plant or an extract derived from a part thereof. Portions include aerial parts (eg, trunks, branches, bark, leaves, flowers, fruits and seeds) or underground parts (eg, roots). The extract derived from a plant belonging to the genus Anogeissus of the present invention is preferably derived from above-ground parts, and more preferably derived from bark.

  In the present specification, plants belonging to the genus Ailanthus (Ailanthus) are not particularly limited as long as they are plants belonging to the genus Ailanthus, and for example, Malabarniwa urushi (Ailanthus triphysa (Dennst.) Alston), Ailanthus altissima (Ailanthus altissima) (Mill.) Swingle) and the like, and preferably, Ailanthus triphisa (Dennst.) Alston.

  The extract derived from a plant belonging to the genus Ailanthus may be an extract derived from the whole plant or may be an extract derived from a part. Portions include aerial parts (eg, trunks, branches, bark, leaves, flowers, fruits and seeds) or underground parts (eg, roots). The extract derived from a plant belonging to the genus Ailanthus of the present invention is preferably derived from above-ground parts, and more preferably derived from branches.

  In the present specification, the plant belonging to the genus Komatsu (Indigofera) is not particularly limited as long as it is a plant belonging to the genus Komatsu (Indigofera), for example, Komatsunagi (Indigofera pseudotinctoria Matsum.), Ginai (Indigofera argentea), Nambang Komatsunagi (Indigofera suffruticosa Mill.), Thai smelt (Indigofera tinctoria L.), elderfish (Indigofera decora Lindl.), Kodachiniwafuji (Indigofera heterantha), and the like, preferably Komatsunagi (Indigofera pseudotinctoria Matsum.).

  An extract derived from a plant belonging to the genus Indigofera may be an extract derived from the whole plant (whole plant) or an extract derived from a part thereof. Portions include aerial parts (eg, stems, leaves, flowers, fruits and seeds) or underground parts (eg, roots). The extract derived from plants belonging to the genus Indigofera of the present invention is preferably derived from whole plants.

  Forsythia of the present invention (Forsythia), Obahimemeo genus (Pouzolzia), Sagaribana (Barringtonia), Anogenesisus (Anogeissus), Aiwatushi (Ailanthus) and Komatsunagi (Indigofera) derived from a plant belonging to a genus selected from the group consisting of. Can be extracted by a known extraction method.

  The plant before the extraction may be collected immediately before the extraction operation, or may be collected in advance, subjected to a drying treatment, and stored. Further, before extraction, it is desirable to appropriately shred or pulverize and use it for extraction. Alternatively, it may be used in extraction after being ground, crushed, or powdered.

The solvent used for the extraction includes an organic solvent. As the extraction solvent, generally, one or more hydrophilic organic solvents selected from ethanol, methanol, acetone and the like or an aqueous solution thereof (aqueous organic solvent) can be used, and among them, methanol is preferable. It is preferable to use a 100% organic solvent as the extraction solvent. When an organic solvent aqueous solution (aqueous organic solvent) is used as the extraction solvent, the organic solvent concentration is 20 to 80%, preferably 30 to 70%, more preferably 40 to 60%, and still more preferably 45 to 55%. It is desirable to use a solvent aqueous solution (a water-containing organic solvent).
The amount of the extraction solvent to be used is 2 to 50 times, preferably 5 to 25 times the amount of the extraction solvent with respect to the plant (weight).

When the extraction is performed at room temperature (10 to 30 ° C.), the extraction may be performed for 1 day or more, preferably 2 days or more, more preferably 3 days or more, further preferably 5 days or more, and particularly preferably 7 days or more. desirable. The upper limit is within 14 days, preferably within 10 days.
When performing extraction under heating conditions (for example, 40 to 80 ° C.), 0.5 to 48 hours, preferably 1 to 24 hours, more preferably 1.5 to 12 hours, still more preferably 2 to 10 hours, and particularly preferably 3 to 8 hours It is preferable to carry out over time.
Although the extraction operation can be performed by standing, it is preferable to perform stirring and mixing. When methanol or hydrated methanol is used as the extraction solvent, the extraction may be carried out by heating and refluxing at a high temperature.

  After the extraction, the extract obtained by separating the solid content from the suspension by filtration or the like can be used as it is or, if necessary, concentrated and dried to use as the hair growth or hair growth promoting agent of the present invention. As used herein, the term “promoting hair growth” refers to shortening the transition period from the telogen to the anagen in the hair cycle. In addition, in this specification, hair growth promotion refers to improving the growth rate of hair. The extract extracted with the above solvent is useful in its purity as the hair growth or hair growth promoting agent of the present invention, but may be further purified by a method known per se.

Further, the extract may be subjected to liquid-liquid extraction using a lipophilic organic solvent after removing the hydrophilic organic solvent and water by performing concentration or the like. Here, examples of the lipophilic organic solvent include ethyl acetate, diethyl ether, benzene, toluene, xylene and the like.
The amount of the lipophilic organic solvent to be used is preferably 0.1 to 3 times, preferably 0.2 to 2 times, more preferably 0.3 to 1 times the amount of the plant (weight). This operation may be performed by stirring and mixing and then allowed to stand.
As the distribution operation, for example, liquid-liquid countercurrent distribution is appropriate. The organic solvent layer is separated, the solvent is distilled off, and the extract is dried to obtain an extract having a hair growth promoting activity and a hair growth promoting activity.

  The extract of the present invention exerts remarkable hair growth and hair growth effects when applied externally to the scalp by the action of a component having hair growth promotion activity and hair growth promotion activity. Therefore, the extract of the present invention can be used as a hair growth or hair growth promoting agent.

  The extract of the present invention contained in the hair growth or hair growth-promoting agent of the present invention includes a Forsythia genus (Forsythia), a genus Obahimemao (Pouzolzia), a genus Sagaribana (Barringtonia), a genus Anogeissus (Anogeissus), a genus Ailanthus and a komatsunagi It may be an extract from at least one plant belonging to the genus selected from the group consisting of the genus (Indigofera). The hair growth or hair growth promoting agent of the present invention includes, for example, an extract derived from two plants, an extract derived from three plants, an extract derived from four plants, and an extract derived from five or more plants. There may be.

  The hair growth or hair growth promoting agent of the present invention includes humans, non-human animals (eg, mammals other than humans (pigs, cattle, horses, dogs, cats, etc.), birds (poultry, such as turkeys and chickens)). It is useful to apply to such as.

The extract of the present invention can be dissolved in fats and oils such as ethanol and camellia oil, cream and the like, and used as a hair growth or hair growth promoting agent.
The amount of the extract is, as solid content, 0.001% (w / w) or more, preferably 0.01 (w / w) or more, more preferably 0.1% (w / w) of the total weight of the hair growth or hair growth promoter. It is preferable to mix them in the proportions of w) or more. If the amount is too small, the effect of promoting hair growth or hair growth is not sufficiently obtained, which is not preferable.
The upper limit is, for example, 5% (w / w) or less, preferably 2% (w / w) or less.

  Further, as other raw material components to be added to the hair growth or hair growth promoting agent, those applied to a normal hair growth or hair growth promoting agent can be used. For example, hydrocarbons, waxes, fats and oils, esters, higher fatty acids, higher alcohols, surfactants, fragrances, dyes, preservatives, preservatives, excipients, cooling agents, wetting agents, antioxidants , UV protection agents, alcohols, pH adjusters, and the like. In addition, a keratolytic agent for the epidermis, a blood circulation promoter, a cell activator, a local stimulant, and the like, which are other hair growth or hair growth promoters, can be added. Further, a bactericide, an anti-inflammatory agent, an antiseborrheic agent, an antipruritic agent and the like can be added.

  The hair growth or hair growth promoting agent of the present invention may be in any form as long as it can be applied externally to the scalp. For example, as a drug or a quasi-drug, it can be in the form of a cream, ointment, lotion or the like.

The hair growth or hair growth-promoting agent of the present invention can also be blended as a component of a hair cosmetic, a hairdressing agent, or a hair washing agent. For example, it can be blended into a hair cream, a hair tonic, a hair lotion, a hair liquid, a hair conditioner, a hair treatment, a hair spray, a shampoo, a rinse, a shampoo and a rinse.
In this case, the content is desirably adjusted in accordance with the range of the compounding amount described above for the hair growth or hair growth promoting agent. If the amount is too small, the effect of promoting hair growth or hair growth is not sufficiently obtained, which is not preferable.
In addition, as other raw material components to be blended in hair cosmetics, hair styling products, and hair washes, raw material components used in normal production can be blended.

  Hereinafter, the present invention will be more specifically described with reference to examples, but the present invention is not limited thereto.

Example 1 Evaluation of hair growth activity
C3H / HeN mice are known to have a dormant period of the dorsal hair cycle from 45 days after birth. The telogen usually lasts up to 90 days after birth, after which the hair cycle enters the anagen phase. In this example, by shaving the back body hair of a C3H / HeN mouse whose hair cycle is telogen, a test compound was applied to the shaved part, and the transition period from telogen to anagen was measured. The hair growth activity of the test compound was evaluated.

Test Compounds Each part of the plant described in Table 1 was chopped or crushed as a crude drug, dried at 60 ° C. overnight, and weighed 500 g by dry weight. 100% methanol in an amount of 5 to 25 times the dry weight was added to the dried crude drug, and the mixture was allowed to stand at room temperature for one week with occasional stirring to elute the active ingredient. The solution was filtered with filter paper (No. 2), concentrated by an evaporator, and a dry powder was obtained using a freeze dryer. The dry powder was dissolved as a test compound in a solvent (hereinafter, 50% EtOH) in which equal amounts of sterile distilled water and ethanol were mixed, and each test compound-containing solution (1% w / v) (# 1801, # 3126, # 543, # 330, # 133, # 2112). The above 50% EtOH was used as a control.

Experimental Procedure Eight-week-old C3H / HeN male mice (Japan SLC) were acclimated for one week, and then their coat was cut off from the back region. Mice with a shaved skin surface color of pink were divided into test groups (50% EtOH, # 1801, # 3126, # 543, # 330, # 133, # 2112) as mice with a telogen resting period. Was.
Each test compound-containing solution or 50% EtOH described above was applied to the shaved back area of each test group against the hair flow so that the application amount was 6.7 μL / cm ² and 100 μL / animal. Application was performed once / day for 5 days / week.
After shaving, the day when each test compound-containing solution or 50% EtOH was first applied to the mice was defined as day 0, the mice were observed on days 1, 6, 8, 12, 14, 16, and 17 The hair growth activity was evaluated. Evaluation of hair growth activity was performed by observing signs of hair growth in the application area and calculating a score based on the observation result. Signs of hair growth include melanocytes in the hair follicles synthesizing melanin and the color of the skin changes from pink to gray. Therefore, the hair growth activity of each test compound was evaluated by assigning a score based on the area where the color of the skin changed or the area where hair elongation was observed on each observation day. Table 2 shows the relationship between the area where the skin color changes or the area where the hair elongates and the score. Table 3 and FIG. 1 show the average values of the scores on each observation day in each test group.

Example 2 Evaluation of hair growth activity
In C57BL6 / N mice, it is known that when the dorsal body hair is removed, the hair cycle of the removed area is forcibly shifted to the catagen, and as a result, the hair cycle of the removed area is synchronized (Ya-Chieh Hsu). et al., Cell, 144, 92-105, 2011). Approximately 7 days after hair removal, as a sign of anagen, in addition to the production of pigmented hair, the appearance of skin turning gray as the melanocytes in the hair follicles begin to synthesize melanin is observed. Further, about 10 days after hair removal, hair growth in the hair removal area is confirmed. In this example, the hair growth activity of the test compound was determined by depilating the back hair of C57BL6 / N mice, applying a test compound to the depilated portion, and measuring the rate of hair growth per day during the anagen phase. evaluated.

Test Compound Using the same test compound as in Example 1, each test compound-containing solution (1% w / v) (# 1801, # 3126, # 543, # 330, # 133, # 2112) was prepared in the same manner. Prepared. In addition, 50% EtOH was used as a control.

Experimental Procedure A 7-week-old C57BL6 / N female mouse (Japan SLC) was acclimated for 1 week, and then the hair was removed and the depilated skin surface was colored pink in each test group (50% EtOH, # 1801, # 3126). , # 543, # 330, # 133, # 2112).
Each test compound-containing solution or 50% EtOH described above was applied against the hair flow so that the application amount was 6.7 μL / cm ² and 100 μL / animal on the hair-removed back region of each test group. Application was performed once / day for 5 days / week.
After hair removal, each test compound-containing solution or 50% EtOH was first applied to the mice, the mice after hair growth were observed, and the hair growth activity of each test compound was evaluated. Evaluation of hair growth activity was performed by periodically collecting hair in the application area and measuring the average rate of elongation of the mouse back body hair per day between day 4 and day 12 after hair growth. Table 4 shows the average value of the elongation rate in each test group.

  Forsythia of the present invention (Forsythia), Obahimemeo (Pouzolzia), Sagaribana (Barringtonia), Anogenesisus (Anogeissus), Aiwatushi (Ailanthus), an extract derived from a plant belonging to the genus Komatsunagi (Indigofera), the hair cycle of the extract. Since it has the effect of shortening the transition period from the telogen to the growth period (hair growth promoting effect) and the effect of increasing the hair growth rate (hair growth promoting effect), it can be used as a hair growth or hair growth promoting agent.

Claims (12)

A hair growth or hair growth-promoting agent , comprising an extract derived from the aerial part of Forsythia japonica Makino . Further comprising an extract from at least one plant belonging to a genus selected from the group consisting of the genus Ouzima (Pouzolzia), the genus Sagaribana (Barringtonia), the genus Anogeissus (Anogeissus), the genus Ailanthus and the genus Komatsuna (Indigofera) The hair growth or hair growth promoting agent according to claim 1. The hair growth or hair growth promoting agent according to claim 2 , wherein the extract derived from a plant belonging to the genus Pouzolzia is an extract derived from above-ground parts. Oobahimemao genus plant belonging to (Pouzolzia) is Otogirimao (Pouzolzia pentandra (Roxb.) Benn .) Is, hair growth or hair growth promoting agent according to claim 2 or 3. The hair growth or hair growth promoting agent according to claim 2 , wherein the extract derived from a plant belonging to the genus Barringtonia is an extract derived from bark. The hair growth or hair growth promoting agent according to claim 2 or 5 , wherein the plant belonging to the genus Barringtonia is Barringtonia pedicellata Ridl. The hair growth or hair growth-promoting agent according to claim 2 , wherein the extract derived from a plant belonging to the genus Anogeissus is an extract derived from bark. The hair growth or hair growth promoting agent according to claim 2 or 7 , wherein the plant belonging to the genus Anogeissus is Yong (Anogeissus acuminate Wall.). The hair growth or hair growth promoting agent according to claim 2 , wherein the extract derived from a plant belonging to the genus Ailanthus is an extract derived from a branch. The hair growth or hair growth-promoting agent according to claim 2 or 9 , wherein the plant belonging to the genus Ailanthus is Ailanthus triphysa (Dennst.) Alston. 3. The hair growth or hair growth promoting agent according to claim 2 , wherein the extract derived from a plant belonging to the genus Indigofera is an extract derived from a whole plant. The hair growth or hair growth promoting agent according to claim 2 or 11 , wherein the plant belonging to the genus Komatsunagi (Indigofera) is Komatsunagi (Indigofera pseudotinctoria Matsum.).