CN116211771B - Plant extract with hair loss prevention and hair growth promotion effects and application thereof - Google Patents
Plant extract with hair loss prevention and hair growth promotion effects and application thereof Download PDFInfo
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- 239000000419 plant extract Substances 0.000 title claims abstract description 39
- 230000000694 effects Effects 0.000 title claims abstract description 14
- 230000003779 hair growth Effects 0.000 title claims abstract description 11
- 230000003658 preventing hair loss Effects 0.000 title claims abstract description 8
- 235000001978 Withania somnifera Nutrition 0.000 claims abstract description 26
- 241000490229 Eucephalus Species 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 239000000706 filtrate Substances 0.000 claims description 54
- 238000002156 mixing Methods 0.000 claims description 43
- 239000000284 extract Substances 0.000 claims description 42
- 238000000605 extraction Methods 0.000 claims description 32
- 238000001914 filtration Methods 0.000 claims description 27
- 210000004209 hair Anatomy 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 24
- 241000586313 Withania Species 0.000 claims description 20
- 201000004384 Alopecia Diseases 0.000 claims description 16
- 239000000047 product Substances 0.000 claims description 12
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 230000003405 preventing effect Effects 0.000 claims description 10
- 241000207925 Leonurus Species 0.000 claims description 9
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 9
- 230000003676 hair loss Effects 0.000 claims description 9
- 208000024963 hair loss Diseases 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 claims description 7
- 235000000802 Leonurus cardiaca ssp. villosus Nutrition 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 229940015975 1,2-hexanediol Drugs 0.000 claims description 5
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 230000003450 growing effect Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 abstract description 16
- 240000004482 Withania somnifera Species 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 230000003656 anti-hair-loss Effects 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract 1
- -1 stilbene glucoside Chemical class 0.000 description 7
- 231100000360 alopecia Toxicity 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- SASUFNRGCZMRFD-JCUIILOWSA-N withanolide D Chemical compound C1C(C)=C(C)C(=O)O[C@H]1[C@](C)(O)[C@@H]1[C@@]2(C)CC[C@@H]3[C@@]4(C)C(=O)C=C[C@H](O)[C@@]54O[C@@H]5C[C@H]3[C@@H]2CC1 SASUFNRGCZMRFD-JCUIILOWSA-N 0.000 description 5
- 230000003698 anagen phase Effects 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 208000003251 Pruritus Diseases 0.000 description 3
- 230000007803 itching Effects 0.000 description 3
- 210000004761 scalp Anatomy 0.000 description 3
- 239000002453 shampoo Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000700157 Rattus norvegicus Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000980780 Cladosiphon okamuranus Species 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010022971 Iron Deficiencies Diseases 0.000 description 1
- 241000555688 Malassezia furfur Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010039793 Seborrhoeic dermatitis Diseases 0.000 description 1
- 208000006981 Skin Abnormalities Diseases 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002454 curcuma longa l. root extract Substances 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 230000002951 depilatory effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000031774 hair cycle Effects 0.000 description 1
- 208000003532 hypothyroidism Diseases 0.000 description 1
- 230000002989 hypothyroidism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 208000008742 seborrheic dermatitis Diseases 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000003797 telogen phase Effects 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 229940025703 topical product Drugs 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/002—Preparations for repairing the hair, e.g. hair cure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Natural Medicines & Medicinal Plants (AREA)
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- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
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- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
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- Alternative & Traditional Medicine (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a plant extract with an anti-hair loss and hair growth promoting effect and application thereof, and relates to the technical field of plant extracts. The plant extract of the invention comprises the following raw materials: fructus forsythiae, withania root and aster in the weight ratio of 0.5-1 to 1-2 to 0.5-1. The natural plant components are extracted, and after screening and testing, the plant extract composition capable of improving the hair loss prevention and hair growth effects is obtained, the effects of the plant extract composition are obviously better than those of single components, and the plant extract composition is safe to use and has no stimulation and good application prospect.
Description
Technical Field
The invention relates to the technical field of plant extracts, in particular to a plant extract with an anti-hair loss and hair-growing effect and application thereof.
Background
Up to 100,000-150,000 hairs were counted on the scalp of the person and their respective growth cycles repeated. The hair growth cycle has three phases: anagen phase, in which hair is most actively growing; the hair begins the degenerative phase of the degeneration; hair growth is stopped and enters the resting, primordial phase. About 15% of the total scalp hair is in the telogen and allergy phases and people typically lose 50-100 hairs per day when new hairs are grown simultaneously.
In general, hair loss refers to a state where no hair is present in a region where hair is normally present, because abnormally shed hair increases in number as the proportion of anagen hair becomes smaller, and the proportion of anagen hair becomes smaller or the proportion of anagen hair increases and decreases. Previously, hair loss was considered as a series of aging phenomena, but with the current social development, the phenomena of hair loss, such as increased stress, staying up, poor scalp blood circulation, excessive androgen secretion, seborrheic dermatitis, genetic factors, nutritional deficiencies, iron deficiencies, drugs, hypothyroidism, etc., are all causes of hair loss, and hair loss phenomena are becoming more common and younger. Therefore, there is an increasing demand for hair loss prevention and hair growth products.
Currently, plant extracts are of interest due to their natural, safe nature, for example, chinese patent application CN202210884041.6 discloses a bacteriostatic, antipruritic and alopecia-preventing composition, and its preparation and application, said composition comprising at least: 1-5 parts by weight of a Cladosiphon okamuranus extract; 1-5 parts by weight of turmeric root extract; and 0.1 to 0.5 part by weight of stilbene glucoside. The invention provides a composition with obviously improved antibacterial speed, itching relieving and alopecia preventing effects, the antibacterial rate of the composition on malassezia furfur in 30min reaches 99.97%, the antibacterial effect is excellent, and the composition contains unstable chemical components but unexpectedly has good stability as a whole. The invention also provides a shampoo containing the composition with obviously improved antibacterial speed, itching relieving and alopecia preventing effects, and the shampoo has good effects of removing dandruff, relieving itching and preventing alopecia.
The alopecia preventing products in the market at present are mainly daily chemical products of ginger components, and the effective and safe components with alopecia preventing effect still remain to be developed and perfected.
Disclosure of Invention
Therefore, the invention aims to provide the plant extract with the hair loss preventing and growing effects and the application thereof, natural plant components are extracted, and after screening and testing, the plant extract composition capable of improving the hair loss preventing and growing effects is obtained, the effects of the plant extract composition are obviously higher than those of single components, and the plant extract composition is safe and non-irritating in use and has good application prospects.
In order to achieve the above object, the present invention has the following technical scheme:
in one aspect, the invention provides a plant extract with hair loss prevention and hair growth promoting effects, which comprises the following raw materials: fructus forsythiae, radix Withania somnifera and radix Asteris.
When the fructus forsythiae, the withania root and the aster are selected from plant materials, the plant extracts are obtained by mixing and extracting; when fructus forsythiae, withania root and aster are extracts, the plant extracts are combined by the extracts.
Preferably, the weight ratio of fructus forsythiae, withania root and aster is 0.5-1:1-2:0.5-1, most preferably 1:2:1.
Preferably, the raw materials further comprise motherwort.
Further preferably, the weight ratio of fructus forsythiae, withania root, motherwort and aster is 0.5-1:1-2:1.5-2:0.5-1, most preferably 1:2:2:1.
In still another aspect, the present invention provides a method for preparing the above plant extract, comprising the steps of:
(1) Mixing fructus forsythiae, radix Withaniae, herba Leonuri and radix Asteris, and pulverizing to obtain extract;
(2) Mixing the extract with water, extracting for the first time, and filtering to obtain residue 1 and filtrate 1;
(3) Mixing the filter residue 1 with butanediol, extracting for the second time, and filtering to obtain filter residue 2 and filtrate 2;
(4) Mixing the filter residue 2 with p-hydroxyacetophenone and 1, 2-hexanediol, extracting for the third time, and filtering to obtain filtrate 3;
(5) Mixing filtrate 1, filtrate 2 and filtrate 3, and concentrating to obtain plant extract.
Preferably, the feed liquid ratio (unit g: ml) of the first extraction is 1:10-20, and the feed liquid ratio of the second extraction and the third extraction is 1:20-40.
Further preferably, the ratio of feed liquid (unit g: ml) at the time of the first extraction is 1:18, and the ratio of feed liquid at the time of the second extraction and the third extraction is 1:30.
Preferably, the temperature of the first extraction is 50-80 ℃; the temperature of the second extraction is 30-50deg.C; the temperature of the third extraction is 25-50deg.C.
Further preferably, the temperature of the first extraction is 60 ℃; the temperature of the second extraction is 45 ℃; the temperature of the third extraction was 32 ℃.
Preferably, the time of the first extraction is 40-120min; the second extraction time is 30-80min; the temperature of the third extraction is 30-80min.
Further preferably, the time of the first extraction is 60min; the second extraction time is 50min; the temperature of the third extraction was 40min.
Preferably, in step (4), the volume ratio of hydroxyacetophenone to 1.2-hexanediol is from 1:2 to 10, more preferably 1:4.
Meanwhile, in the experimental process, the applicant respectively prepares single-component extracts from fructus forsythiae, withania root, motherwort and aster according to the similar steps and then mixes the single-component extracts, and the mixed extraction is preferred in view of shortening the operation time and saving the extraction solvent without obvious difference in effect.
In a final aspect, the present invention provides the use of the above plant extract for the preparation of a product having anti-hair loss and hair-growing efficacy.
Preferably, the product is a topical product.
Such topical products include, but are not limited to, shampoos, conditioners, essences, essential oils, ointments, sprays, and the like.
Preferably, the content of the above plant extract in the product is not less than 0.05%.
Further preferably, the content of the above plant extract in the product is not less than 3%.
The beneficial effects of the invention are as follows:
(1) Natural plant components are extracted, so that the components are safe and not stimulated, and the use safety is improved;
(2) The plant extract composition has good hair loss prevention and hair growth promoting effects, and has better effect compared with a single component extract.
Detailed Description
In order to make the technical means, the creation features, the achievement of the purpose and the effect of the present invention easy to understand, the present invention will be further elucidated with reference to the specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all of them. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the invention. In the following examples, unless otherwise specified, the methods of operation used were conventional, the equipment used was conventional, and the materials used in the examples were the same. Fructus forsythiae, withania root and motherwort used in the invention are located in Gansu province, and aster is located in Anhui province.
Example 1
(1) Pulverizing fructus forsythiae, mixing with water at a feed-liquid ratio of 1:18, extracting at 60deg.C for 1 hr, and filtering to obtain residue 1 and filtrate 1;
(2) Mixing the filter residue 1 with butanediol, extracting at a feed-liquid ratio of 1:20 at a temperature of 42 ℃ for 40min, and filtering to obtain filter residue 2 and filtrate 2;
(3) Mixing the filter residue 2 with p-hydroxyacetophenone-1, 2-hexanediol (1:4), extracting at 30deg.C for 40min at a feed-liquid ratio of 1:30, extracting for the third time, and filtering to obtain filtrate 3;
(4) Mixing filtrate 1, filtrate 2 and filtrate 3, and concentrating to obtain fructus forsythiae fruit extract.
Example 2
(1) Pulverizing radix Withania somnifera, mixing with water, extracting at 60deg.C for 1 hr at a feed-liquid ratio of 1:18, and filtering to obtain residue 1 and filtrate 1;
(2) Mixing the filter residue 1 with butanediol, extracting at 50 ℃ for 1h according to a feed-liquid ratio of 1:20, and filtering to obtain filter residue 2 and filtrate 2;
(3) Mixing the filter residue 2 with p-hydroxyacetophenone-1, 2-hexanediol (1:4), extracting at 35deg.C for 40min at a feed-liquid ratio of 1:30, extracting for the third time, and filtering to obtain filtrate 3;
(4) Mixing filtrate 1, filtrate 2 and filtrate 3, and concentrating to obtain dry extract.
Example 3
(1) Pulverizing radix Asteris, mixing with water, extracting at 60deg.C for 1 hr at a feed-liquid ratio of 1:18, and filtering to obtain residue 1 and filtrate 1;
(2) Mixing the filter residue 1 with butanediol, extracting at 50deg.C for 50min at a feed-liquid ratio of 1:20, and filtering to obtain filter residue 2 and filtrate 2;
(3) Mixing the filter residue 2 with p-hydroxyacetophenone-1, 2-hexanediol (1:4), extracting at 30deg.C for 40min at a feed-liquid ratio of 1:30, extracting for the third time, and filtering to obtain filtrate 3;
(4) Mixing filtrate 1, filtrate 2 and filtrate 3, and concentrating to obtain dry extract.
Example 4
Raw materials: 1 part of fructus forsythiae, 2 parts of withania root and 1 part of aster.
(1) Mixing fructus forsythiae, radix Withania somnifera and radix Asteris, and pulverizing to obtain extract;
(2) Mixing the extract with water, extracting at 60deg.C for 1 hr at a feed-liquid ratio of 1:18, and filtering to obtain residue 1 and filtrate 1;
(3) Mixing the filter residue 1 with butanediol, extracting at 45 ℃ for 50min at a feed-liquid ratio of 1:30, and filtering to obtain filter residue 2 and filtrate 2;
(4) Mixing the filter residue 2 with p-hydroxyacetophenone-1, 2-hexanediol (1:4), extracting at 32deg.C for 40min at a feed liquid ratio of 1:30, extracting for the third time, and filtering to obtain filtrate 3;
(5) And (3) mixing the filtrate 1, the filtrate 2 and the filtrate 3, and concentrating to obtain a dry extract to obtain the plant extract.
Example 5
Raw materials: 1 part of fructus forsythiae, 1 part of withania root, and 1 part of aster.
The preparation method is the same as in example 4.
Example 6
Raw materials: fructus forsythiae 0.5 weight parts, withania root 2 weight parts and aster 0.5 weight parts.
The preparation method is the same as in example 4.
Example 7
Raw materials: 1 part of fructus forsythiae, 2 parts of withania root, 1 part of aster and 1 part of motherwort.
The preparation method is the same as in example 4 (step (1) in which all the raw materials of the formulation are mixed and crushed).
Comparative example 1
Raw materials: 1 part of fructus forsythiae, 2 parts of withania root and 1 part of aster.
(1) Mixing fructus forsythiae, radix Withania somnifera and radix Asteris, and pulverizing to obtain extract;
(2) Mixing the extract with water, extracting at 60deg.C for 1 hr at a feed-liquid ratio of 1:18, and filtering to obtain residue 1 and filtrate 1;
(3) Mixing the filter residue 1 with butanediol, extracting at 45 ℃ for 50min at a feed-liquid ratio of 1:30, and filtering to obtain filter residue 2 and filtrate 2;
(4) Mixing the filter residue 2 with ethanol, extracting at 32deg.C for 40min at a feed-liquid ratio of 1:30, extracting for the third time, and filtering to obtain filtrate 3;
(5) And (3) mixing the filtrate 1, the filtrate 2 and the filtrate 3, and concentrating to obtain a dry extract to obtain the plant extract.
Comparative example 2
Raw materials: 1 part of fructus forsythiae, 2 parts of withania root and 1 part of aster.
(1) Mixing fructus forsythiae, radix Withania somnifera and radix Asteris, and pulverizing to obtain extract;
(2) Mixing the extract with water, extracting at 60deg.C for 1 hr at a feed-liquid ratio of 1:18, and filtering to obtain residue 1 and filtrate 1;
(3) Mixing the filter residue 1 with butanediol, extracting at 45 ℃ for 50min at a feed-liquid ratio of 1:30, and filtering to obtain filter residue 2 and filtrate 2;
(4) Mixing the filter residue 2 with p-hydroxyacetophenone-1, 2-hexanediol (1:2), extracting at 32deg.C for 40min at a feed liquid ratio of 1:30, extracting for the third time, and filtering to obtain filtrate 3;
(5) And (3) mixing the filtrate 1, the filtrate 2 and the filtrate 3, and concentrating to obtain a dry extract to obtain the plant extract.
Comparative example 3
Raw materials: 2 parts of fructus forsythiae, 0.5 part of withania root and 2 parts of aster.
The preparation method is the same as in example 4.
Result detection
1. Experimental animals and groupings: clean-class Wistar rats weighing 180-210g, females, 160 total, were housed under normal conditions for one week, randomly grouped, 10 per group.
2. The experimental method comprises the following steps: the extracts prepared in the examples and comparative examples were administered in groups according to the following table by selecting a flat area of 3cm x 3cm on the back of each mouse, and applying the extracts of each group to the depilatory area after preparing the extracts of each group into a solution with a mass concentration of 10%, wherein the application amount is 25mg/kg, each time in the morning and evening, and the blank group was applied with distilled water of the same mass. After one week, each mouse was injected with 160mg/kg cyclophosphamide in a single dose, and the administration was continued for 2 weeks in the manner described above, the hair in the dehaired area was removed, the hair length, hair weight, skin abnormality were recorded, and the mice were fed with water freely during the experiment.
3. The experimental results are as follows:
note that: p < 0.05, p < 0.01 compared to the blank group
4. Discussion of results
From the results in the table it can be seen that:
(1) The mixed extracts of fructus forsythiae, withania root and aster and the mixture of the extracts of the three have good effects of preventing alopecia and growing hair, and can be used in hair washing and hair care products.
(2) The results of single extracts of fructus forsythiae, withania root and aster with the concentration of 10% are not obvious, and meanwhile, the results of single extract of fructus forsythiae, withania root, single extract of fructus forsythiae, and aster, single extract of fructus forsythiae, are 1:2, 1:1, and 2:1 are improved to a certain extent relative to the single extract, but the improvement degree is not obvious.
(3) The experimental results of the single fructus forsythiae extract, the single withano root extract and the single aster root extract = 1:2:1 group prove that the compound composition of the three extracts remarkably improves the hair length and the hair weight of a Wistar rat, and the results show that the single fructus forsythiae extract, the single withano root extract and the single aster root extract have synergistic effects in the aspect of hair loss prevention and hair growth.
(4) The mixed extracts of examples 4-7 all obviously improve the hair loss preventing and hair growing effects, p is less than 0.01, and the hair length and the hair weight are further improved after the motherwort herb is added. In addition, the mode of mixed extraction is finally selected in view of shortening the operation time and saving the extraction solvent.
(5) The technical effect of the comparative examples 1 to 3 is also remarkably improved, but compared with the example 4, the volume ratio of the hydroxyacetophenone to the 1.2-hexanediol is 1:4, which is most favorable for the extraction of the active ingredients, and the anti-hair loss and hair growth effects are further improved, so that the volume ratio of the hydroxyacetophenone to the 1.2-hexanediol is preferably 1:4.
(6) The plant extract is safe and nonirritant, does not have adverse reactions such as redness, spots, roughness and the like after being used for 3 weeks, and is milder when being applied to daily chemical products.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (7)
1. The plant extract with the hair loss prevention and hair growth effects is characterized in that the raw materials of the plant extract comprise the following components: fructus forsythiae, withania root and aster in a weight ratio of 0.5-1:1-2:0.5-1; the raw materials also comprise motherwort;
the preparation method of the plant extract comprises the following steps:
(1) Mixing fructus forsythiae, radix Withaniae, herba Leonuri and radix Asteris, and pulverizing to obtain extract;
(2) Mixing the extract with water, extracting for the first time, and filtering to obtain residue 1 and filtrate 1;
(3) Mixing the filter residue 1 with butanediol, extracting for the second time, and filtering to obtain filter residue 2 and filtrate 2;
(4) Mixing the filter residue 2 with p-hydroxyacetophenone and 1, 2-hexanediol, extracting for the third time, and filtering to obtain filtrate 3; in the step (4), the volume ratio of the hydroxyacetophenone to the 1, 2-hexanediol is 1:2-10;
(5) Mixing filtrate 1, filtrate 2 and filtrate 3, and concentrating to obtain plant extract.
2. The plant extract according to claim 1, wherein the weight ratio of fructus forsythiae, withania root and aster is 1:2:1.
3. The plant extract according to claim 1, wherein the weight ratio of fructus forsythiae, withania root, leonurus and aster is 0.5-1:1-2:1.5-2:0.5-1.
4. A plant extract according to claim 3, wherein the weight ratio of fructus forsythiae, withania root, leonurus and aster is 1:2:2:1.
5. The method for preparing a plant extract according to any one of claims 1 to 4, comprising the steps of:
(1) Mixing fructus forsythiae, radix Withaniae, herba Leonuri and radix Asteris, and pulverizing to obtain extract;
(2) Mixing the extract with water, extracting for the first time, and filtering to obtain residue 1 and filtrate 1;
(3) Mixing the filter residue 1 with butanediol, extracting for the second time, and filtering to obtain filter residue 2 and filtrate 2;
(4) Mixing the filter residue 2 with p-hydroxyacetophenone and 1, 2-hexanediol, extracting for the third time, and filtering to obtain filtrate 3, wherein in the step (4), the volume ratio of the hydroxyacetophenone to the 1, 2-hexanediol is 1:2-10;
(5) Mixing filtrate 1, filtrate 2 and filtrate 3, and concentrating to obtain plant extract.
6. The method according to claim 5, wherein the ratio of feed to liquid g/mL in the first extraction is 1:10-20, and the ratio of feed to liquid in the second extraction and the third extraction is 1:20-40; the temperature of the first extraction is 50-80 ℃; the temperature of the second extraction is 30-50deg.C; the temperature of the third extraction is 25-50deg.C; the first extraction time is 40-120min; the second extraction time is 30-80min; the temperature of the third extraction is 30-80min.
7. Use of the plant extract according to any one of claims 1 to 4 for preparing a product having hair loss preventing and hair growing effects, wherein the content of the plant extract in the product is not less than 0.05%.
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