JP2016183137A - Hair growth or hair restoration promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a hair growth or hair restoration promoter.SOLUTION: The present invention provides a hair growth or hair restoration promoter that comprises extract derived from at least one plant selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera.SELECTED DRAWING: None

Description

  The present invention relates to an extract having hair growth promoting activity and hair growth promoting activity obtained from a plant. More particularly, at least belonging to the genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera. The present invention relates to a hair growth or hair growth promoter containing an extract derived from one plant as an active ingredient.

  Hair repeats growth and loss according to a periodic hair cycle consisting of a growth phase, a regression phase, and a resting phase. Of these hair cycles, the stage at which new hair follicles are formed from the resting stage to the growing stage is considered to be the most important for hair growth. In this stage, the proliferation and differentiation of hair follicle epithelial cells are considered. It is believed that the dermal papilla cells play an important role. The dermal papilla cell is a cell located inside the hair follicle epithelial cell composed of outer root sheath cells and matrix cells in the vicinity of the hair root, and located in the root portion of the hair root wrapped in the basement membrane. It plays an important role in the growth and differentiation of hair follicle epithelial cells and the formation of hair, such as by promoting the proliferation of epithelial cells.

  A state in which the hair cycle is stagnant or shortened is called alopecia, and alopecia that is routinely seen in the medical field includes not only alopecia areata associated with aging and alopecia areata associated with mental stress, but also cancer and endocrinology. Hair loss caused by diseases such as abnormalities and drugs such as anticancer agents is known. Since hair loss greatly affects the patient's appearance, the development of a fundamental treatment and prevention method is desired.

  Finasteride or minoxidil is commercially available as a drug used for the treatment or prevention of alopecia, but it is known that finasteride has side effects such as sexual dysfunction, and minoxidil has itching and inflammation.

  On the other hand, plant extracts that have been used for a long time in folk remedies and traditional medicine (eg, Kampo medicine) have the advantage that they can be taken or ingested safely for a long period of time with little risk of side effects. Yes. For this reason, research on active ingredients derived from plants has been actively conducted in Japan and overseas in the field of pharmaceuticals and foodstuffs, and plant extracts that can be used for the prevention or treatment of alopecia are also subject to search. It has become. For example, it has been reported that an extract of mulberry skin known to have an antitussive action, a diuretic action, and the like has hair growth activity (Patent Document 1).

  Under such circumstances, hair growth or hair growth promoters that are more effective and have no side effects are desired.

JP 2014-218479 A

  An object of this invention is to provide the hair growth or the hair growth promoter which contains the plant-derived extract as an active ingredient.

  The inventor of the present invention is an extract derived from a plant belonging to the genus Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus, Indigofera. The inventors discovered for the first time that they had the effect of shortening the transition period from the rest period of the cycle to the growth period and the effect of increasing the hair elongation rate, and completed the hair growth or hair growth promoter containing the extract.

That is, the present invention
[1] At least one member belonging to the genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera Hair growth or hair growth promoter comprising an extract from one plant;
[2] The hair growth or hair growth promoter according to [1], wherein the plant-derived extract belonging to the genus Forsythia is an extract derived from the above-ground part;
[3] The hair growth or hair growth promoter according to [1] or [2], wherein the plant belonging to Forsythia is Forsythia japonica Makino;
[4] The hair growth or hair growth promoter according to [1], wherein the plant-derived extract belonging to the genus Pouzolzia is an extract derived from the aerial part;
[5] The hair growth or hair growth promoter according to [1] or [4], wherein the plant belonging to the genus Pouzolzia is Pouzolzia pentandra (Roxb.) Benn.
[6] The hair growth or hair growth promoter according to [1], wherein the plant-derived extract belonging to the genus Barringtonia is a bark-derived extract;
[7] The hair growth or hair growth promoter according to [1] or [6], wherein the plant belonging to the genus Barringtonia is Barringtonia pedicellata Ridl.
[8] The hair growth or hair growth promoter according to [1], wherein the plant-derived extract belonging to the genus Anogeissus is a bark-derived extract;
[9] The hair growth or hair growth promoting agent according to [1] or [8], wherein the plant belonging to the genus Anogeissus is Anogeissus acuminata Wall.
[10] The hair growth or hair growth promoter according to [1], wherein the plant-derived extract belonging to the genus Ailanthus is a branch-derived extract;
[11] The hair growth or hair growth promoter according to [1] or [10], wherein the plant belonging to the genus Ailanthus is Ailanthus triphysa (Dennst.) Alston;
[12] The hair growth or hair growth promoter according to [1], wherein the plant-derived extract belonging to the genus Indigofera is an extract derived from whole grass;
[13] The hair growth or hair growth promoter according to [1] or [12], wherein the plant belonging to the genus Indigofera is Indigofera pseudotinctoria Matsum.
[14] The hair growth or hair growth promoter according to any one of [1] to [13], wherein the extract is an extract extracted with an organic solvent;
[15] The hair growth or hair growth promoter according to [14], wherein the organic solvent is 100% methanol;
I will provide a.

  From at least one plant belonging to the genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera Can be provided as a hair growth or hair growth promoter.

It is a graph which shows the average value of the score of each observation day in each test group.

  The present invention is at least a genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera. A hair growth or hair growth promoting agent comprising an extract derived from one plant is provided.

  In the present specification, the plant belonging to the genus Forsythia is not particularly limited as long as it belongs to the genus Forsythia, for example, Forsythia japonica Makino, Forsythia suspensa (Thunb.) Vahl), Forsythia viridissima Lindl., Forsythia viridissima Lindl. Var. Koreana Rehder, and the like, preferably Forsythia japonica Makino.

  The extract derived from a plant belonging to the Forsythia genus may be an extract derived from the whole plant or an extract derived from a part thereof. Some include the aerial parts (eg trunks, branches, bark, leaves, flowers, fruits and seeds) or the underground parts (eg roots). The plant-derived extract belonging to the genus Forsythia of the present invention is preferably derived from the above-ground part, and if it is a young tree, it is preferable to use the entire above-ground part, and if it is an adult tree, it uses branches and leaves.

  In the present specification, the plant belonging to the genus Pouzolzia is not particularly limited as long as it is a plant belonging to the genus Pouzolzia, for example, Pougolzia pentandra (Roxb.) Benn., Pouzolzia zeylanica. (L.) Benn.), And the like, preferably Pouzolzia pentandra (Roxb.) Benn.

  An extract derived from a plant belonging to the genus Pouzolzia may be an extract derived from the whole plant or an extract derived from a part thereof. Some include the aerial parts (eg trunks, branches, bark, leaves, flowers, fruits and seeds) or the underground parts (eg roots). The plant-derived extract belonging to the genus Pouzolzia of the present invention is preferably derived from the above-ground part, and if it is a young tree, the whole above-ground part is preferably used, and if it is an adult tree, it is preferable to use branches and leaves.

  In the present specification, the plant belonging to the genus Barringtonia is not particularly limited as long as it belongs to the genus Barringtonia, for example, Barringtonia pedicellata Ridl., Barringtonia asiatica (L .) Kurz) and the like, and preferably Barringtonia pedicellata Ridl.

  The extract derived from a plant belonging to the genus Barringtonia may be an extract derived from the whole plant or an extract derived from a part thereof. Some include the aerial parts (eg trunks, branches, bark, leaves, flowers, fruits and seeds) or the underground parts (eg roots). The plant-derived extract belonging to the genus Barringtonia of the present invention is preferably derived from the above-ground part, and more preferably derived from bark.

  In the present specification, the plant belonging to the genus Anogeissus is not particularly limited as long as it is a plant belonging to the genus Anogeissus. Yon (Anogeissus acuminata Wall.) Is preferable.

  An extract derived from a plant belonging to the genus Anogeissus may be an extract derived from the whole plant or an extract derived from a part thereof. Some include the aerial parts (eg trunks, branches, bark, leaves, flowers, fruits and seeds) or the underground parts (eg roots). The plant-derived extract belonging to the genus Anogeissus of the present invention is preferably derived from the above-ground part, and more preferably derived from bark.

  In the present specification, the plant belonging to the genus Ailanthus is not particularly limited as long as it is a plant belonging to the genus Ailanthus, for example, Ailanthus triphysa (Dennst.) Alston, Ailanthus altissima (Mill.) Swingle) and the like, preferably Ailanthus triphisa (Dennst.) Alston.

  The extract derived from a plant belonging to the genus Ailanthus may be an extract derived from the whole plant or an extract derived from a part thereof. Some include the aerial parts (eg trunks, branches, bark, leaves, flowers, fruits and seeds) or the underground parts (eg roots). The plant-derived extract belonging to the genus Ailanthus of the present invention is preferably derived from the above-ground part, and more preferably derived from a branch.

  In the present specification, the plant belonging to the genus Indigofera is not particularly limited as long as it is a plant belonging to the genus Indigofera, for example, Indigofera pseudotinctoria Matsum., Indigofera argentea, Namban Komatsuna (Indigofera suffruticosa Mill.), Taiwan Komatsunagi (Indigofera tinctoria L.), Niwafuji (Indigofera decora Lindl.), Kodachiniwafuji (Indigofera heterantha), etc., and Komatsunagi (Indigofera pseudotinctoria Matsum.) Is preferred.

  The plant-derived extract belonging to the genus Indigofera may be an extract derived from the whole plant (whole plant) or an extract derived from a part thereof. Some include the aerial parts (eg stems, leaves, flowers, fruits and seeds) or the underground parts (eg roots). The plant-derived extract belonging to the genus Indigofera of the present invention is preferably derived from whole plants.

  Derived from a plant belonging to the genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera of the present invention The extract can be extracted by a known extraction method.

  The plant before extraction may be collected immediately before the extraction operation, or may be collected in advance and stored after being subjected to a drying treatment or the like. Moreover, before extraction, it is desirable to use it for extraction after appropriately chopped or pulverized. Alternatively, it may be used for extraction after being ground, crushed, or powdered.

An organic solvent is mentioned as a solvent used for extraction. As the extraction solvent, one or more hydrophilic organic solvents selected from ethanol, methanol, acetone and the like or an aqueous solution thereof (water-containing organic solvent) can be used. Of these, methanol is preferable. It is preferable to use a 100% organic solvent as the extraction solvent. When an organic solvent aqueous solution (hydrous organic solvent) is used as the extraction solvent, the organic solvent concentration is 20 to 80%, preferably 30 to 70%, more preferably 40 to 60%, still more preferably 45 to 55%. It is desirable to use a solvent aqueous solution (hydrous organic solvent).
The amount of the extraction solvent used is 2 to 50 times, preferably 5 to 25 times the amount of the extraction solvent relative to the plant (weight).

The extraction time is 1 day or more, preferably 2 days or more, more preferably 3 days or more, further preferably 5 days or more, particularly preferably 7 days or more when performed at room temperature (10 to 30 ° C.). desirable. The upper limit is within 14 days, preferably within 10 days.
When the extraction is performed under heating conditions (for example, 40 to 80 ° C.), 0.5 to 48 hours, preferably 1 to 24 hours, more preferably 1.5 to 12 hours, further preferably 2 to 10 hours, and particularly preferably 3 to 8 hours. It is preferable to carry out over time.
The extraction operation may be left still, but it is desirable to perform stirring and mixing. Moreover, when using methanol or hydrous methanol as an extraction solvent, you may carry out by heating-refluxing at high temperature.

  After the extraction, the extract obtained by separating the solid content from the suspension by filtration or the like can be used as it is or after concentration and drying as necessary, as the hair growth or hair growth promoter of the present invention. In this specification, hair growth promotion means shortening the transition period from the resting phase to the growth phase in the hair cycle. Moreover, in this specification, hair growth promotion means improving the growth rate of hair. The extract extracted with the above solvent is useful as a hair growth or hair growth promoter of the present invention because of its purity, but may be further purified by a method known per se.

Further, the extract may be subjected to liquid-liquid extraction using a lipophilic organic solvent after concentration or the like is performed to remove the hydrophilic organic solvent or moisture. Examples of the lipophilic organic solvent include ethyl acetate, diethyl ether, benzene, toluene, xylene and the like.
The amount of the lipophilic organic solvent used is preferably 0.1 to 3 times, preferably 0.2 to 2 times, more preferably 0.3 to 1 times the amount of the plant (weight). The operation may be performed after stirring and mixing.
As the distribution operation, for example, liquid-liquid countercurrent distribution is appropriate. When the organic solvent layer is collected, and the solvent is distilled off and dried, an extract having hair growth promoting activity and hair growth promoting activity is obtained.

  The extract of the present invention exhibits remarkable hair growth and hair growth effects when applied externally to the scalp due to the action of components having hair growth promotion activity and hair growth promotion activity. Therefore, the extract of the present invention can be used as a hair growth or hair growth promoter.

  The extract of the present invention contained in the hair growth or hair growth promoting agent of the present invention includes Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Komatsunagi. It may be an extract from at least one plant belonging to the genus selected from the group consisting of the genus (Indigofera). The hair growth or hair growth promoter of the present invention includes, for example, two plant-derived extracts, three plant-derived extracts, four plant-derived extracts, and five or more plant-derived extracts. There may be.

  The hair growth or hair growth promoter of the present invention is a human, non-human animal (eg, non-human mammals (domestic animals such as pigs, cows, horses, dogs, cats), birds (turkeys such as turkeys and chickens), etc.) Etc. are useful.

The extract of the present invention can be dissolved in oils and fats such as ethanol and camellia oil, creams and the like and used as a hair growth or hair growth promoter.
The blending amount of the extract is 0.001% (w / w) or more, preferably 0.01 (w / w) or more, more preferably 0.1% (w / w) of the total weight of the hair growth or hair growth promoter in terms of solid content. It is preferable to blend in the proportion of w) or more. When the blending amount is too small, the effect of promoting hair growth or hair growth cannot be sufficiently obtained, which is not preferable.
Examples of the upper limit include 5% (w / w) or less, preferably 2% (w / w) or less.

  Moreover, as another raw material component mix | blended with the said hair growth or hair growth promoter, what is applied to normal hair growth or a hair growth promoter can be used. For example, hydrocarbons, waxes, fats and oils, esters, higher fatty acids, higher alcohols, surfactants, fragrances, pigments, preservatives, preservatives, excipients, cooling agents, wetting agents, antioxidants , UV protective agents, alcohols, pH adjusters and the like can be blended. In addition, other epidermal keratolytic agents, blood circulation promoters, cell activators, local stimulants, and the like, which are other hair growth or hair growth promoters, can be blended. Moreover, a disinfectant, an anti-inflammatory agent, an antiseborrheic agent, an antidiarrheal agent, etc. can be mix | blended.

  The hair growth or hair growth promoter of the present invention may be in any form as long as it can be externally applied to the scalp. For example, it can be in the form of cream, ointment, lotion, etc. as a medicine or quasi drug.

The hair growth or hair growth-promoting agent of the present invention can also be blended as a component of a cosmetic product for hair, a hair styling product, or a hair washing product. For example, it can be blended in hair cream, hair tonic, hair lotion, hair liquid, hair conditioner, hair treatment, hair spray, shampoo, rinse, shampoo and rinse.
The content in this case is desirably adjusted according to the range of the blending amount mentioned in the hair growth or hair growth promoter. When the blending amount is too small, the effect of promoting hair growth or hair growth cannot be sufficiently obtained, which is not preferable.
In addition, the raw material component used for normal manufacture can be mix | blended as another raw material component mix | blended with cosmetics for hair, a hairdressing agent, and a hair-washing agent.

  In the following, the present invention will be more specifically described with reference to examples, but the present invention is not limited thereto.

Example 1 Evaluation of hair growth activity
In C3H / HeN mice, it is known that the hair cycle of the dorsal body hair enters a resting phase from 45 days after birth. The rest period usually lasts until 90 days after birth, after which the hair cycle enters the growth period. In this example, by shaving the back body hair of C3H / HeN mice whose hair cycle is at rest, applying a test compound to the shaved portion, and measuring the transition period from rest to growth The hair growth activity of the test compound was evaluated.

Each part of the plant described in Table 1 as a test compound crude drug was shredded or crushed, dried overnight at 60 ° C., and weighed 500 g in dry weight. 100% methanol of 5 to 25 times the dry weight was added to the dry herbal medicine and allowed to stand at room temperature for 1 week with occasional stirring to elute the active ingredients. The solution was filtered through a filter paper (No. 2), concentrated by an evaporator, and a dry powder was obtained using a freeze dryer. Using the dry powder as a test compound, each of the test compound-containing solutions (1% w / v) (# 1801, # 3126, # 543, # 330, # 133, # 2112). As a control, the above 50% EtOH was used.

Experimental Procedure After acclimating an 8-week-old C3H / HeN male mouse (Japan SLC) for 1 week, the fur coat was cut from the back region. Mice whose shaved skin surface is pink are divided into test groups (50% EtOH, # 1801, # 3126, # 543, # 330, # 133, # 2112) as mice with a resting hair cycle. It was.
Each test compound-containing solution or 50% EtOH was applied against the hair flow so that the application amount was 6.7 μL / cm ² and 100 μL / animal on the shaved back region of each test group. Application was carried out once / day for 5 days / week.
The day when the mice were shaved and each test compound-containing solution or 50% EtOH was first applied to the mice was defined as day 0, and the mice were observed on days 1, 6, 8, 12, 14, 16, 17 The hair growth activity was evaluated. The evaluation of hair growth activity was carried out by observing signs of hair growth in the application area and calculating a score based on the observation results. As an indication of hair growth, melanocytes in the hair follicle synthesize melanin and the skin color changes from pink to gray. Therefore, the hair growth activity of each test compound was evaluated by assigning a score based on the area where the skin color changed or the area where hair elongation was observed on each observation day. Table 2 shows the relationship between the skin color change area or the hair extension area and the score. Moreover, the average value of the score of each observation day in each test group is shown in Table 3 and FIG.

Example 2 Evaluation of Hair Growth Activity
In C57BL6 / N mice, it is known that when the dorsal body hair is removed, the hair cycle of the hair removal region is forcibly shifted to the regression phase, and as a result, the hair cycle of the hair removal region is synchronized (Ya-Chieh Hsu et al., Cell, 144, 92-105, 2011). About 7 days after hair removal, as a sign of the growth period, it is observed that the skin turns gray as melanocytes in the hair follicle begin to synthesize melanin in addition to the production of pigmented hair. Furthermore, hair growth in the hair removal region is confirmed about 10 days after hair removal. In this example, the back hair of C57BL6 / N mice was depilated, a test compound was applied to the depigmented portion, and the hair growth activity of the test compound was measured by measuring the hair growth rate per day during the growth phase. evaluated.

Test compound Using the same test compound as in Example 1, each test compound-containing solution (1% w / v) (# 1801, # 3126, # 543, # 330, # 133, # 2112) was prepared in the same manner. Prepared. As a control, 50% EtOH was used.

Experimental procedure Seven-week-old C57BL6 / N female mice (Japan SLC) were acclimated for one week, and then hair was removed, and mice with hair color pink were removed from each test group (50% EtOH, # 1801, # 3126). , # 543, # 330, # 133, # 2112).
Each test compound-containing solution or 50% EtOH was applied against the hair flow so that the application amount was 6.7 μL / cm ² and 100 μL / animal to the back region of each test group. Application was carried out once / day for 5 days / week.
First, each test compound-containing solution or 50% EtOH was applied to the mice, and the mice after hair growth were observed to evaluate the hair-growth activity of each test compound. Evaluation of hair growth activity was carried out by periodically collecting hair in the application area and measuring the average elongation rate of the mouse back body hair per day during the 4th to 12th days after hair growth. Table 4 shows the average value of the elongation rate in each test group.

  An extract derived from a plant belonging to the genus Forsythia, the genus Pouzolzia, the genus Barringtonia, the genus Anogiesus, the genus Ailanthus, and the genus Indigofera has a hair cycle. Since it has the effect of shortening the transition period from the rest period to the growth period (hair growth promoting effect) and the effect of increasing the hair elongation rate (hair growth promoting effect), it can be used as a hair growth or hair growth promoting agent.

Claims (15)

  From at least one plant belonging to the genus selected from the group consisting of Forsythia, Pouzolzia, Barringtonia, Anogeissus, Ailanthus and Indigofera A hair growth or hair growth promoter comprising an extract of   The hair growth or hair growth-promoting agent according to claim 1, wherein the plant-derived extract belonging to the Forsythia genus is an above-ground extract.   The hair growth or hair growth promoter according to claim 1 or 2, wherein the plant belonging to Forsythia is Forsythia japonica Makino.   The hair growth or hair growth promoter according to claim 1, wherein the plant-derived extract belonging to the genus Pouzolzia is an extract derived from the above-ground part.   The hair growth or hair growth promoter according to claim 1 or 4, wherein the plant belonging to the genus Pouzolzia is Pouzolzia pentandra (Roxb.) Benn.   The hair growth or hair growth promoter according to claim 1, wherein the plant-derived extract belonging to the genus Barringtonia is an extract derived from bark.   The hair growth or hair growth promoter according to claim 1 or 6, wherein the plant belonging to the genus Barringtonia is Barringtonia pedicellata Ridl.   The hair growth or hair growth-promoting agent according to claim 1, wherein the plant-derived extract belonging to the genus Anogeissus is a bark-derived extract.   The hair growth or hair growth promoter according to claim 1 or 8, wherein the plant belonging to the genus Anogeissus is Anogeissus acuminata Wall.   The hair growth or hair growth promoter according to claim 1, wherein the plant-derived extract belonging to the genus Ailanthus is a branch-derived extract.   The hair growth or hair growth promoter according to claim 1 or 10, wherein the plant belonging to the genus Ailanthus is Ailanthus triphysa (Dennst.) Alston.   The hair growth or hair growth promoting agent according to claim 1, wherein the plant-derived extract belonging to the genus Indigofera is an extract derived from whole grass.   The hair growth or hair growth promoter according to claim 1 or 12, wherein the plant belonging to the genus Indigofera is Indigofera pseudotinctoria Matsum.   The hair growth or hair growth promoter according to any one of claims 1 to 13, wherein the extract is an extract extracted with an organic solvent.   The hair growth or hair growth promoter according to claim 14, wherein the organic solvent is 100% methanol.