JP6675878B2 - Topical composition for hair growth and hair growth - Google Patents

Description

  The present invention relates to a novel composition for external use for hair growth and hair growth.

  Hair has a role in protecting the head from direct sunlight, external impact, etc., and also plays a major role in creating the appearance and appearance of humans. It is estimated that human hair has an average of about 100,000 hairs, but about 50 to 100 hairs are naturally dropped per day. If the hair continues to fall off significantly more than this, the amount of hair may be abnormally reduced, and a state where the background is visible (ie, thin hair) may occur. Causes of thinning hair have been reported to be due to, for example, heredity, living environment (stress, etc.), skin diseases (excessive sebum secretion, etc.), medications (anticancer drugs, etc.), but many have not been elucidated yet. .

  As a countermeasure for thinning hair, there are a surgical method such as a wig and a hair transplant, a method using an external medicine or an internal medicine. In particular, the method using topical drugs is effective in that it can be easily prescribed by applying it to the head and can be applied to both men and women.

  As an external medicine, a composition containing a component derived from a natural product is widely used, and this is put into practical use as a hair restorer, for example, pharmaceuticals, quasi-drugs, cosmetics (shampoo, rinse, hair cream, etc.) and the like. Sold in form. Various natural products are known, and in particular, a hair restorer using a seaweed extract as an active ingredient has been proposed.

  Examples of such a hair restorer include extraction of three or more types of seaweeds selected from, for example, the family Laminariaceae, the Laminariaceae, the Lessoniaceae, the Larvanaceae, the Honedaraceae, the Myrinaceae, the Laminariaceae, the Mudenoriaceae, the Tenguaceae, the Ogonoriaceae, and the Ulvaceae. A hair papilla cell growth promoter characterized by containing a substance is known (Patent Document 1).

  In addition, prostaglandin A, prostaglandin D and derivatives thereof, adenosine 3 ′, 5′-cyclic-phosphate compound (cAMP) derivative, fucoidan, fucoidan-containing extract, have an odd-numbered carbon chain length There is a hair-growing composition characterized by containing one or more compounds selected from fatty acids and derivatives thereof, alcohols and derivatives thereof having an odd carbon chain length, tocotrienol and minoxidil as active ingredients ( Patent Document 2).

JP 2007-131571 JP 2003-155218 A

  These prior art hair restorers use extracts such as kelp, and although some improvement is recognized, there is room for further improvement.

  Therefore, a main object of the present invention is to provide a hair growth / hair growth composition that can exhibit more excellent hair growth / hair growth effects.

  The present inventor has conducted intensive studies in view of the problems of the prior art, and as a result, has found that the above object can be achieved by using an extract of a gametophyte of seaweed as an active ingredient, and has completed the present invention. Was.

That is, the present invention relates to the following external use composition for hair growth and hair growth.
1. An external composition for hair growth and hair growth containing an extracted component extracted from a gametophyte of algae.
2. Item 2. The external composition for hair growth and hair growth according to Item 1, wherein the solid concentration of the extracted component is 0.01 to 100 mg / mL.
3. Item 3. The external composition for hair growth and hair growth according to Item 1 or 2, wherein the algae are at least one plant that produces gametophytes smaller than spores in the process of heteromorphic alternation.
4. The extracted components are 1) a step of preparing a fine powder of a gametophyte by crushing a gametophyte, 2) a step of obtaining a mixed solution by adding the fine powder to a solvent, and 3) a solid-liquid process of the mixed solution. Item 4. The external composition for hair growth and hair growth according to any one of Items 1 to 3, which is obtained by a production method including a step of collecting a liquid component by separating.
5. Item 4. The external composition for hair growth and hair growth according to Item 4, wherein the step 1) includes a step of previously drying the gametophyte before pulverization.
6. Item 4. The external composition for hair growth and hair growth according to Item 4, further comprising a step of heating the mixture after the step 2) and prior to the step 3).
7. Item 2. A method for producing an external composition for hair growth and hair growth according to Item 1, wherein 1) a step of preparing a fine powder of a gametophyte by crushing a gametophyte, and 2) adding the fine powder to a solvent. A method of producing a composition for external use for hair growth and hair growth, comprising the steps of: obtaining a liquid mixture by subjecting the mixture to solid-liquid separation of the liquid mixture.
8. Item 8. The method according to Item 7, wherein the step 1) includes a step of previously drying the gametes before pulverization.
9. Item 8. The method according to Item 7, further comprising a step of heating the mixed solution after the step 2) and before the step 3).

  ADVANTAGE OF THE INVENTION According to this invention, the composition for hair growth and hair growth which can exhibit more excellent hair growth and hair growth effects can be provided. More specifically, since the composition for external use for hair growth and hair growth of the present invention contains the extracted components extracted from the gametophytes of algae, more excellent hair growth and hair growth effects can be obtained. Although the sporulation of algae has been used as a raw material in hair growth agents and the like so far, in the present invention, by selectively using gametophytes that are separate organisms from the sporulation, the sporulation is higher than when sporulation is used. Hair growth and hair growth effects can be realized. In other words, the extracted components extracted from the gametophytes of algae can be effectively used as the active ingredients of the external composition for hair growth and hair growth of the present invention.

  The composition for external use for hair growth and hair growth according to the present invention having such characteristics can be used, for example, for cosmetics (hair tonic, hair cream, hair lotion, hair liquid, hair treatment, hair mousse, hair shampoo, hair rinse, eyebrows, eyelashes, eyelashes and mustaches) Beauty liquid, etc.), quasi-drugs and pharmaceuticals (external preparations, sprays, patches) and the like.

5 is a graph showing the change over time of the score (hair growth state) measured in Test Example 1. FIG. 9 is a diagram showing a result of photographing a mouse in Test Example 1. It is a graph which shows the result of having measured the hair weight of the mouse | mouth on the 116th day of observation in Test Example 1. 6 is a graph showing changes over time of scores (hair growth area score and hair length score) measured in Test Example 2. FIG. 9 is a view showing a result of photographing a mouse in Test Example 2.

1. External composition for hair growth and hair growth The external composition for hair growth and hair growth of the present invention (composition for external use of the present invention) is characterized by containing, as an active ingredient, an extracted component extracted from a gametophyte of algae.

  The algae is not limited as long as it forms a gametophyte. In particular, it is preferable to use a plant that produces gametophytes (especially, smaller than sporophytes) in the process of heteromorphic alternation. it can. In the present invention, a plant (seaweed) belonging to the brown algae class can be particularly preferably used. For example, in addition to algae belonging to the order Laminaria such as kelp (Water squirrel), seaweed, alame, swordfish, black turtle, etc., algae belonging to the order Azulidae such as Mozuku, etc. Algae belonging to the group. Therefore, in the present invention, for example, algae belonging to the family Laminariaceae can be suitably used.

  For example, in kelp, the edible portion is called a sporophyte, and its growth is performed by spores released from the sporophyte. These spores can swim in the sea as zoospores and become gametophytes by germinating after attaching to rocky places and the like. There are males and females in the gametophytes, sperm and eggs are produced in each, and new sporophytes are born by fertilization of both. In the present invention, such a gametophyte is used as a starting material, which is clearly distinguished from a conventional hair restorer using a spore body as a starting material.

  In the present invention, the sex of the gametophyte is not limited, and both male and female gametes can be employed. For example, it can be appropriately selected according to the degree of the hair growth effect, ease of culture, and the like.

  In addition, a gametophyte obtained by collecting a gametophyte vegetated on a rocky place in the sea can be used, and a gametophyte obtained by artificially growing algae in an aquarium (cultivation or terrestrial culture) is used. be able to.

  The composition for external use of the present invention contains such an extract of gametophytes as an active ingredient. The extract component can be used as it is in the form of a liquid (extract), or can be used as a solid (dry matter). The extraction method will be described in a manufacturing method described later.

  The concentration (solid content concentration) of the extractable component in the composition for external use of the present invention is not limited, but is usually about 0.01 to 100 mg / mL, and particularly preferably 1 to 10 mg / mL. By setting the concentration within the above range, more excellent hair growth and hair growth effects can be obtained, and more excellent storage stability as an external composition can be realized.

  In addition, the composition for external use of the present invention may contain other components as necessary, in addition to the above-mentioned extracted components. For example, known or known solvents (water, water-soluble organic solvents), moisturizing components, dispersants (emulsifiers), surfactants, fragrances, coloring agents, antioxidants, antibacterial agents, thickeners, preservatives, pH adjusters and the like. An additive contained in a commercially available hair restorer or the like may be contained within a range that does not impair the effects of the present invention. In addition, an active ingredient (blood circulation promoting ingredient, anti-itch ingredient, anti-dandruff ingredient, anti-inflammatory ingredient, anti-androgen ingredient, cell activating ingredient, etc.) contained in a known or commercially available hair restorer can also be contained.

  The properties of the composition for external use of the present invention are not particularly limited, but are generally liquids, and may be either solutions or dispersions (emulsions). In addition, from the viewpoint of viscosity, any viscous liquid such as a paste, jelly (gel), or emulsion may be used in addition to a liquid having no viscosity (a liquid having substantially the same viscosity as water). Further, it can be used in the form of an aerosol or the like. As the product form, for example, shampoo, rinse, hair cream, mousse, pack, hair spray and the like can be appropriately adopted.

  The composition for external use for hair growth and hair growth of the present invention can be basically used in the same manner as a known or commercially available external composition for hair growth. For example, as an external preparation for skin, the composition for external use of the present invention can be used by directly applying it to the scalp (affected part). The amount of application (dosage) is usually about 1 to 3 times per day, and may be carried out continuously until the desired effect is obtained. For example, it is preferably used continuously for 3 months or more.

2. Method for producing an externally applied composition for hair growth and hair growth The method for producing the externally applied composition of the present invention is realized by a method including a step of extracting an extraction component from a gametophyte of algae, but the method is not particularly limited, and for example, Various extraction methods such as a method using a solvent and a method using an ion exchange resin can be adopted. Therefore, for example, in the present invention, it can be suitably carried out particularly by the following method.

  That is, in the present invention, 1) a step of preparing a fine powder of a gametophyte by crushing a gametophyte (a crushing step), and 2) a step of obtaining a mixed solution by adding the fine powder to a solvent (a mixing step). 3) a step of heating the mixture to 50 to 90 ° C. (heating step) and 4) a step of collecting a liquid component by solid-liquid separation of the mixture (solid-liquid separation step). A gametophyte extract can be suitably produced.

Pulverizing Step In the pulverizing step, the gametophyte is pulverized to prepare a fine powder of the gametophyte. The method of pulverizing the gametophyte is not particularly limited, and a known method such as pulverization using a mortar or pulverization using a ball mill can be used. In this case, the pulverization method may be either wet pulverization or dry pulverization.

  In the pulverizing step, the gamete can be subjected to a drying treatment in advance, if necessary. Thereby, it becomes possible to carry out pulverization (dry pulverization) more effectively, and furthermore, it is possible to more reliably obtain the desired extractable component of the present invention. The gametophyte may be dried by either natural drying or heat drying. The degree of drying is not limited as long as pulverization is possible, but usually the moisture content may be set to about 3% by weight or less. The lower limit of the water content is not limited, and may include 0% by weight, but may be, for example, about 0.1% by weight.

  The crushing of the gametophyte is performed for smooth extraction by crushing the gametophyte, and can be performed according to a known crushing method. For example, known crushing means such as a mortar, a ball mill, and a jet mill can be employed. The crushing is performed in order to smoothly remove components contained in the gametophyte by crushing the gametophyte, and the degree of crushing is not particularly limited as long as part or all of the gametophyte is destroyed, Usually, the average particle size may be 10 μm or less. The lower limit is not particularly limited, and may be, for example, about 0.1 μm.

Mixing Step In the mixing step, a mixed liquid is obtained by adding the fine powder to a solvent. As a solvent to be used, in addition to water, an organic solvent such as a hydrophilic organic solvent and a lipophilic organic solvent can be used. These solvents can be used alone or in combination.

  As the hydrophilic organic solvent, for example, ethanol, methanol, acetone, dioxane, tetrahydrofuran, isopropanol, n-propanol, propylene glycol, 1,3-butylene glycol, glycerin and the like can be used.

  Examples of the lipophilic organic solvent include n-butanol, n-amyl alcohol, n-hexanol, ethyl acetate, propyl acetate, butyl acetate, methylene chloride, chloroform, carbon tetrachloride, benzene, toluene, xylene, diethyl ether, petroleum ether , N-hexane and the like can be used.

  The concentration (solid content) of the fine powder in the mixed solution is not particularly limited, but can usually be appropriately set in the range of 1 to 50% by weight. By the mixing step (further, a subsequent heating step), at least a part of the components contained in the fine powder is eluted into the solvent.

Warming Step In the present invention, after the mixing step and prior to the solid-liquid separation step, the mixed liquid can be heated to about 50 to 90 ° C, preferably 60 to 80 ° C, if necessary. By heating within the above temperature range, it is possible to more efficiently extract the extracted components from the gametophyte. In addition, the heating holding time may be a time sufficient for the components contained in the fine powder to elute into the solvent, but is usually about 0.5 to 5 hours.

Solid-Liquid Separation Step In the solid-liquid separation step, a liquid component (filtrate) is recovered by subjecting the mixed liquid to solid-liquid separation. The method of solid-liquid separation may be in accordance with a known method. For example, various methods and apparatuses such as a filter press, centrifugal separation, and ultrafiltration can be employed.

  In this case, the recovered liquid component can be used as an active ingredient of the composition for external use of the present invention as it is or after adjusting its concentration. When the recovered liquid component is used after its concentration is adjusted, for example, it can be diluted by adding a solvent. As the solvent, the various solvents described above can be used.

  In addition, the liquid component can be used to recover a dissolved component (solute) in the liquid, if necessary, and use the dissolved component as an active component of the composition for external use of the present invention. When a dissolved component is recovered from a liquid component, the dissolved component can be further dissolved or dispersed in a solvent before use. As the solvent, the various solvents described above can be used.

  In preparing the composition for external use of the present invention, various additives can be blended as necessary. Examples of the additives include various components (moisturizing components, dispersants (emulsifiers), surfactants, fragrances, coloring agents, antioxidants, antibacterial agents, thickeners, and the like) contained in known or commercially available hair restorers and the like. A commercially available hair-growth component, etc.) can be blended within a range that does not impair the effects of the present invention. In addition, various active ingredients (blood circulation promoting ingredient, anti-itch ingredient, anti-dandruff ingredient, anti-inflammatory ingredient, anti-androgen ingredient, cell activating ingredient, etc.) contained in known or commercially available hair restorer can also be contained. .

  Hereinafter, examples and comparative examples are shown, and the features of the present invention will be described more specifically. However, the scope of the present invention is not limited to the examples.

Example 1
As the algae, Laminaria religiosa Miyabe, which belongs to the family Laminariaceae, was used. This was grown in a water tank, and after the gametophytes were collected, drying was performed. The obtained dried powder was crushed in a mortar to obtain a fine powder. A liquid mixture was prepared by adding 20 parts by weight of 70% ethanol (anhydrous ethanol prepared with ultrapure water to a concentration of 70 v / v%) to 1 part by weight of the fine powder, and then 70 ° C. The mixture was allowed to stand for 1 hour while being heated. Then, after cooling to room temperature, the mixture was centrifuged at 4 ° C. with a centrifugal force of 15000 g for 30 minutes, followed by filtration to perform solid-liquid separation. The solid content of the liquid component obtained by the solid-liquid separation was measured. Next, Sample 1 was obtained by adjusting the concentration with the same ethanol as above so that the solid concentration of the liquid obtained by the solid-liquid separation was 10 mg / mL. Sample 1 was dispensed in a single use amount and stored at -20 ° C under light shielding until use.

Test example 1
An evaluation test of hair growth and hair growth was performed using Sample 1 prepared in Example 1. A mouse (C3H / HeN CrlCrlj, male, 6 weeks old) (manufactured by Charles River Japan Co., Ltd.) was shaved with a clipper under pentobarbital anesthesia. The shaving range was from the neck (right and left ear roots) to the buttocks (tail), and the hair weight after shaving was measured. The weight of the mouse before and after shaving was also measured. Next, every day from the day after the shaving treatment, Sample 1 was applied to the shaved area of the mouse at regular intervals. The application amount was 0.1 mL / mouse (1 mg / mouse as the amount of the extracted component). In this test example, eight mice were used for each administration group. In addition, upon observation of the hair growth state, the hair growth area and hair length were scored on a six-point scale from 0 to 5. The scoring criteria are shown in Table 1.

  For comparison, as shown in Table 2, it was obtained in the same manner as in Example 1 except that 1) a mouse to which 70% ethanol itself was applied (Comparative Example 1), and 2) a kelp spore body was used. Mice coated with a sample prepared using the extracted components (Comparative Example 2), 3) Minoxidil, a commercially available hair growth agent component (manufactured by Tokyo Chemical Industry Co., Ltd.), and the concentration thereof was adjusted to 10 mg / mL. Eight mice each of which were continuously applied with a sample (Comparative Example 3) obtained by adjusting the concentration with 70% ethanol were prepared daily, and these were similarly observed daily. The dose in Table 2 indicates the daily dose of each component.

  The hair growth area and hair length of the mice in each of these groups were observed and measured daily, and photographs of the mice were taken under non-anesthesia every week from the time when hair growth was observed. The results of the “hair growth state” score calculated from the hair growth area and hair length scores are shown in FIG. The calculation method was [(hair growth area score) × (hair length score) / 5]. FIG. 2 shows the result of photographing each mouse on the 102nd day (“photograph” in FIG. 1). In FIG. 1, points indicated by symbols such as * indicate points that are significant. Further, the hair weight of each mouse on day 116 was measured. The result is shown in FIG. In these figures, "Konbu gametophyto" refers to Example 1, "Control" refers to Comparative Example 1, "Konto spore body" refers to Comparative Example 2, and "minoxidil" refers to Comparative Example 3, respectively.

  As is clear from these results, it is understood that the mouse of Example 1 is excellent in the hair growth promoting effect and the hair growth promoting effect as compared with Comparative Examples 1 to 3. In particular, in Example 1, the hair growth promoting effect appeared from an early stage on the 53rd day, and even in relation to Comparative Example 2 using the spores of kelp, a more excellent effect as a hair restorer or the like was achieved. We can see that we can do it.

Example 2
A sample was prepared in the same manner as in Example 1 except that black algae (Ecklonia kurome Okamura) belonging to the family Laminariaceae were used as the algae (sample 2).

Test example 2
An evaluation test for hair growth and hair growth was performed using Sample 2 prepared in Example 2. Using the same mice as in Test Example 1, the same shaving treatment as in Test Example 1 was performed. Then, every day from the day after the shaving treatment, Sample 2 was applied to the shaved area of the mouse at regular intervals. The application amount was 0.1 mL / mouse (1 mg / mouse as the amount of the extracted component). In this test example, eight mice were used for each administration group. Further, in observing the hair growth state, the hair growth area and hair length were scored in six stages from 0 to 5 in the same manner as in Table 2.

  For comparison, as shown in Table 3, it was obtained in the same manner as in Example 2 except that 1) a mouse to which 70% ethanol itself was applied (Comparative Example 4), and 2) a black spore body. Mouse coated with a sample prepared using the extracted component (Comparative Example 5), 3) Minoxidil, a commercially available hair growth component (manufactured by Tokyo Chemical Industry Co., Ltd.), and the concentration thereof was adjusted to 10 mg / mL. Eight mice each of which were continuously applied with a sample (Comparative Example 6) obtained by adjusting the concentration with 70% ethanol were prepared every day, and these were similarly observed daily. The dose in Table 3 indicates the daily dose of each component.

  The hair growth area and hair length of the mice in each of these groups were observed and measured daily, and photographs of the mice were taken under non-anesthesia every week from the time when hair growth was observed. FIG. 4 shows the results of the scores for the hair growth area and hair length. FIG. 5 shows the result of photographing each mouse on the 74th day (“photograph” in FIG. 4). In FIG. 4, points indicated by symbols such as * indicate points that are significant. In these figures, "Kome gametophyte" refers to Example 2, "Control" refers to Comparative Example 4, "Kurome sporophyte" refers to Comparative Example 5, and "minoxidil" refers to Comparative Example 6, respectively.

  As is clear from these results, the mouse of Example 2 is superior to the hair growth promoting effect and the hair growth promoting effect as compared with Comparative Examples 4 to 6. In particular, in Example 2, the hair growth-promoting effect appears from the early stage on the 65th day, and even in comparison with Comparative Example 5 using the black spores, a more excellent effect as a hair restorer can be achieved. I understand.

Claims (3)

An external composition for hair growth and hair growth comprising an extractive component extracted from a gametophyte of Hosome kelp or Kurome . The composition for external use for hair growth and hair growth according to claim 1, wherein the solid content concentration of the extracted component is 0.01 to 100 mg / mL. The composition for external use for hair growth and hair growth according to claim 1 or 2, wherein the composition is a liquid .