KR101953257B1 - Composition for preventing hair loss and growing hair comprising tulip, saw palmetto, nettle extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss and promoting the proliferation of human dermal papilla cells, and proliferation of keratinocytes, and a production method therefor. More particularly, the present invention relates to a composition comprising extracts of Tulipa gesneriana, Serenoa repens, and Urtica dioica as active ingredients, and to a method, comprising the composition as an active ingredient, for producing a composition for preventing hair loss and promoting proliferation of human dermal papilla cells and proliferation of keratinocyte. In addition, the composition exhibits an excellent hair loss prevention effect and hair growth promoting effect on hair loss and hair growth inhibition caused by stress and inflammation in the body. Since a natural raw material having no toxicity is used as an active ingredient, the composition can be used as a hair loss preventing agent, a scalp protective agent and a hair growth promoting agent for protecting the scalp and preventing hair loss.

Description

Hair loss prevention and hair growth promoting composition comprising a tulip extract, saw palmetto extract and nettle extract as an active ingredient {COMPOSITION FOR PREVENTING HAIR LOSS AND GROWING HAIR COMPRISING TULIP, SAW PALMETTO, NETTLE EXTRACTS}

The present invention relates to a cosmetic composition for preventing hair loss and promoting hair growth and a method for manufacturing the same, and more particularly, to a cosmetic composition for promoting hair loss prevention, anti-inflammatory action, hair follicle cell activation and hair growth. It provides a method for producing a cosmetic composition comprising an extract consisting of soil, nettle and the like as an active ingredient.

Hair and scalp are parts of the body as they age and appear aging like skin, and as aging progresses, various changes and problems appear. Like nails, hair is a change in skin tissue, has a hair cycle, repeats birth-growth-rest-hair loss, and loses about 60 to 80 averages per day on healthy scalp. In essence, hair is composed of a protein called keratin, so once damaged, it can't be completely restored. It is important not to overdo it by choosing the proper chemicals when performing perma or dyeing. It is also important to repair and maintain damaged hair.

On the other hand, in recent years, the demand of women and young people who demand hair loss prevention on a daily basis rather than promoting hair growth is increasing rapidly, and in accordance with the flow to the composition containing the hair loss prevention active ingredient in the shampoo composition for daily use There are a number of patents, Korean Patent Publication No. 10-2011-0040100 is a hair or scalp cosmetic composition for preventing hair loss and promoting the wool containing herbal extracts and the Republic of Korea Patent Publication No. 10-2008-0096663 Although a cosmetic composition for hair containing is disclosed, the results of such studies show a clear clinical effect, but the mechanism of action is often not clearly specified, and the necessity of research on this continues.

Republic of Korea Patent Publication No. 10-2011-0040100 Republic of Korea Patent No. 10-2008-0096663

The present invention contains saw palmetto extract, which is well known as a health functional plant, and tulip and nettle extracts having excellent anti-inflammatory and soothing effects as active ingredients to promote induction from the resting phase to the growing hair cycle and to maintain the growing hair cycle for a long time. It provides a hair or scalp cosmetic composition that has a wool promoting effect and is safe for the human body even after long-term use.

However, the problem to be solved by the present application is not limited to the above-mentioned problem, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

One aspect of the present application provides a cosmetic composition comprising a tulip extract, saw palmetto extract and nettle extract.

Another aspect of the present application provides a cosmetic comprising the cosmetic composition.

The cosmetic composition of the present invention promotes cell proliferation of human dermal papilla cells and human keratinocytes and ultimately exhibits a very good effect on preventing hair loss and promoting hair growth in vivo. In addition, the cosmetic composition of the present invention can be used for the development of drugs and quasi-drugs that are safe for humans because they use natural ingredients without toxicity as active ingredients.

Figure 1 shows the degree of hair growth in the mouse before the control, minoxidil (μoxidil) 0.01 μM and the cosmetic composition of Examples 1 to 4 to the epidermis of the mouse.
Figure 2 shows the degree of hair growth in the mouse 14 days after the control, minoxidil (Minoxidil) 0.01 μM and the cosmetic composition of Examples 1 to 4 after application to the epidermis of the mouse.
Figure 3 is a control, minoxidil 0.01 μM and the cosmetic composition of Examples 1 to 4 after 14 days after application to the epidermis of the mouse, when stained with hematoxylin (Hematoxylin) and Iosin (Eosin) A photograph of the skin tissue of the mouse observed.

Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present disclosure. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted in order to clearly describe the present invention.

Throughout this specification, when a part is said to "include" a certain component, it means that it can further include other components, without excluding the other components unless specifically stated otherwise.

Hereinafter, with reference to the accompanying drawings will be described embodiments of the present application; However, the present application is not limited to these embodiments and drawings.

One aspect of the present application provides a cosmetic composition comprising a tulip extract, saw palmetto extract and nettle extract.

Another aspect of the present application provides a cosmetic comprising the cosmetic composition.

Another aspect of the present application provides a shampoo, hair rinse, hair tonic, hair gel, hair lotion, hair pack, hair spray, hair mousse, hair treatment and hair dye as a cosmetic comprising the cosmetic composition.

In one embodiment of the present application, the cosmetic composition may be a cosmetic composition, when the weight ratio of the tulip extract is 1, the weight ratio of the saw palmetto extract and the nettle extract is mixed in a ratio of 0.5 to 2, but is not limited thereto. .

In one embodiment of the present application, the cosmetic composition may be a cosmetic composition in which a tulip extract, saw palmetto extract and nettle extract are mixed in a weight ratio of 1: 1: 2, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto. The preparation method of the tulip extract, saw palmetto extract and nettle extract of the present invention is as follows.

Comparative Example 1 Preparation of Tulip Extract

10 g of purified water was added to 100 g of dried tulip (purchased from Asen Korea Co., Ltd., Bupyeong-gu, Incheon) and extracted under reflux for 4 hours at 90 to 100 ° C. The extract was filtered with filter paper (Whatman, Dassel, Germany), and then concentrated under reduced pressure at 60 ° C using a conventional concentrator (EYELA, Japan). Thereafter, the concentrate was lyophilized to obtain 22.4 g of tulip extract by dry weight, which was then used as a test sample.

[Comparative Example 2] Saw Palmetto Preparation of Extract

To 100 g of dried saw palmetto (purchased from Asen Korea Co., Ltd., Bupyeong-gu, Incheon), 10-fold purified water was extracted and refluxed at 90 to 100 ° C for 4 hours. The extract was filtered with filter paper (Whatman, Dassel, Germany), and then concentrated under reduced pressure at 60 ° C using a conventional concentrator (EYELA, Japan). Thereafter, the concentrate was freeze-dried to obtain 27.1 g of saw palmetto extract as a dry weight, which was then used as a test sample.

Comparative Example 3 Preparation of Nettle Extract

10 g of purified water was added to 100 g of dried nettle (purchased from Herb Town, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do) and refluxed at 90-100 ° C. for 4 hours. The extract was filtered with filter paper (Whatman, Dassel, Germany), and then concentrated under reduced pressure at 60 ° C using a conventional concentrator (EYELA, Japan). Thereafter, the concentrate was freeze-dried to obtain 29.5 g of a nettle extract as a dry weight, which was used as the following experimental sample.

Example 1 Preparation of a mixture in which the ratio of each extract is 1: 1: 1

The tulip extract, saw palmetto extract and nettle extract of Comparative Examples 1 to 3 were mixed in a weight ratio of 1: 1: 1, and then used as the following experimental samples.

Example 2 Preparation of a Mixture with Each Extract Ratio 2: 1: 1

The tulip extract, saw palmetto extract and nettle extract of Comparative Examples 1 to 3 were mixed in a weight ratio of 2: 1: 1, and then used as the following experimental samples.

Example 3 Preparation of a Mixture with Each Extract Ratio 1: 2: 1

The tulip extract, saw palmetto extract and nettle extract of Comparative Examples 1 to 3 were mixed at a weight ratio of 1: 2: 1, and then used as the following experimental samples.

Example 4 Preparation of a Mixture with Each Extract Ratio of 1: 1: 2

The tulip extract, saw palmetto extract and nettle extract of Comparative Examples 1 to 3 were mixed in a weight ratio of 1: 1: 2, and then used as the following experimental samples.

Experimental Example 1 Proliferation Promoting Effect of Human Hair Papillary Cells

Human Hair Dermal Papilla Cells (Science Cell Research Laboratories Co., USA) were inoculated into 96 well plates to 1.5 X 10 ⁴ cells / well, followed by 10% fetal bovine serum (Gibco Co., USA). ) Was incubated for 24 hours in a 37%, 5% CO ₂ incubator using DMEM (Gibco Co., USA) medium.

Thereafter, each test substance was diluted with DMEM medium containing no fetal bovine serum and treated for 24 hours at 0 (control), 25, 50, and 100 μg / mL for each concentration, followed by 5 mg / mL MTT (3- ( 4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) solution was added to a final concentration of 1 mg / mL and further incubated for 4 hours.

Finally, the supernatant was discarded and DMSO (Sigma Co., USA) was added to each well of 200 μL to dissolve the crystal violet in a shaking incubator (TW3, Fine PRC, South Korea) for 30 minutes, followed by microplate reader (BIO-TEK Instruments Inc.). , Power Wave X340, USA) was used to measure the absorbance at 570 nm. The effect of each test substance on the proliferation of human dermal papilla cells was expressed in% of the absorbance of the control group not treated with each test substance, Minoxidil was used as a positive control to add reliability of the experimental results.

Proliferation promoting effect of the human dermal papilla cells can be confirmed through Table 1 below.

TABLE 1

As shown in the results of Table 1, it was confirmed that the proliferation rate of human dermal papilla cells is increased in the treatment of the positive control group, human dermal papilla cells depending on the comparative concentrations of Comparative Examples 1 to 3 and Examples 1 to 4 of the present invention. It was confirmed that the growth of, and the growth rate of human dermal papilla cells showed a tendency to increase approximately 10% when the treatment concentration was increased from 25 μg / mL to 100 μg / mL. In addition, it was confirmed that the growth of human dermal papilla cells is most promoted when the extract of Example 4, extract of tulip, somal metho, and nettle extract at a weight ratio of 1: 1: 2. In addition, while the saw palmetto extract of Comparative Example 2 can be confirmed that the most inferior effect of human dermal papilla cells, Example 2 containing the tulip extract, the nettle extract in addition to containing only the saw palmetto extract of Comparative Example 2 The growth rate tends to increase by approximately 20% compared to that of Comparative Example 2, and it can be seen that the synergistic effect of the mixing of each extract.

Experimental Example 2 Proliferation Promoting Effect of Human Keratinocytes

After inoculating human keratinocytes (HaCaT Cells; ATCC, USA) into a 96 well plate to be 1 × 10 ⁵ cells / well, DMEM containing 10% fetal bovine serum (Gibco Co., USA) Gibco Co., USA) media was used to incubate for 24 hours in 37 ℃, 5% CO ₂ incubator. Thereafter, each test substance was diluted with DMEM medium containing no fetal bovine serum, treated with 0 (control), 25, 50, and 100 μg / mL for 24 hours, and then 5 mg / mL of MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) solution was added to a final concentration of 1 mg / mL and further incubated for 4 hours. Finally, the supernatant was discarded, DMSO (Sigma Co., USA) was added to each well of 200 μL, and the crystal violet was dissolved in a shaking incubator (TW3, Fine PRC, South Korea) for 30 minutes, followed by microplate reader (BIO-TEK Instruments Inc.). , Power Wave X340, USA) was used to measure the absorbance at 570 nm. The effect of each test substance on the proliferation of human keratinocytes was expressed as a percentage of the absorbance of the control group not treated with each test substance, and Minoxidil was used as a positive control to increase the reliability of the experimental results.

Proliferation promoting effect of the human keratinocytes can be confirmed through Table 2.

TABLE 2

As shown in the results of Table 2, it was confirmed that the proliferation rate of human keratinocytes was also significantly increased in the positive control group, as in the human dermal papilla cells of Comparative Examples 1 to 3 and Examples 1 to 4 of the present invention. It was confirmed that the proliferation of human keratinocytes was promoted depending on the treatment concentration, and when the treatment concentration was increased from 25 μg / mL to 100 μg / mL, the proliferation rate of human keratinocytes increased by about 10%.

In addition, it can be seen that the most keratinocyte proliferation is promoted when the extract of Tulip, Somal Meto, and Nettle extract of Example 4 is treated at a weight ratio of 1: 1: 2, as in Comparative Examples 1 to 3, respectively. When the extract is used alone, the mixture of Examples 1 to 4 is higher than the mixture of Examples 1 to 4, it can be seen that the synergistic effect is greater, each extract of Examples 1 to 4 than when used alone Proliferation rate of keratinocytes tends to increase by approximately 20% when in the form of a mixture, and it can be confirmed that they have a synergistic effect when the respective extracts are mixed. In addition, it can be confirmed that the embodiment of the present invention shows the same efficacy as Minoxidil being used as the main ingredient of the quasi-drug.

Experimental Example 3 Embryonic Growth Inducing Effect in Mouse Animal Model

All animals used in the experiment were males of 5 weeks old C57BL / 6 mice, and a total of 36 animals were purchased from Orient Bio (Seongnam, Gyeonggi-do, Korea). The animals purchased were subjected to a week-long acclimation period in a laboratory with a temperature of 21 ± 3 ° C, a relative humidity of 50 ± 5% and an illuminance of 200-300 lux. No adverse reactions were observed.

Drinking water and feed should be provided sterile but not limited to intake. In order to check the abnormality of the test animals, body weights were measured daily during the test period as well as during the adaptation period, but no weight loss was observed.

For hair removal of C57BL / 6 mice, hair was first removed using an electric hair clipper for animals, and then again, a hair removal agent of chioglycolic acid (Hanyang, Nare Cream) was applied and hair was completely removed with a soft cotton wool after 1 minute. The remaining hair removal agent was completely removed by using a cotton pad with water, and each sample was applied from 2 days after hair removal. The application was carried out for 14 days, and only negative solvents (50% ethanol, 30% butylene glycol, 20% distilled water) were used to dissolve the sample.

As shown in the results of FIGS. 1 and 2, the hair growth promoting effect of the treatment groups of Examples 1 to 4 was much better than that of the negative control group. In addition, they showed a similar degree of hair growth promoting effect as the minoxidil (Sigma Co., USA) treatment group, which is currently used as a hair loss-related medicine, and side effects such as weight loss and skin irritation were observed in all experimental groups. Not observed.

Therefore, the composite extract including the tulip extract, saw palmetto extract and nettle extract of the present invention was confirmed to be a safe material with excellent hair growth promoting effect.

Experimental Example 4 Embryonic Growth Promotion Effect through Histological Analysis

After anesthesia was injected by injecting a sufficient amount of 4% Chloral Hydrate (Chloral hydrate) to the experimental animals, the skin area to which the sample was applied was extracted with an anatomical instrument. The extracted skin tissue was fixed with 10% formalin for 2 days, and then dehydrated step by step using alcohol and xylene, and then embedded in paraffin. Tissue embedded in paraffin was prepared into 5 μm sections using a microtome, and then paraffin was removed with xylene and hydrated step by step with alcohol. Finally, the skin sections were stained with hematoxylin and eosin to observe the changes in hair follicles in the skin tissue, and then examined by light microscopy.

As shown in the results of Figure 3, the positive control group (Minoxidil), Examples 1 to 4 treatment group compared to the negative control group, the hair follicles are more active, the size and length of the hair root was observed to grow. In particular, they were able to confirm that the epidermis (Epidermis) layer proliferation more actively than the negative control, the overall thickness of the skin becomes thick.

Therefore, the complex extract containing the tulip extract, saw palmetto extract and nettle extract as an active ingredient not only promotes the growth of human dermal papilla cells and human keratinocytes but also promotes hair growth phase induction than when used alone. Not only did it work, but its excellent growth promoting effect was confirmed by various experiments using cells and animals.

The above description of the present application is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive.

The scope of the present application is indicated by the following claims rather than the above description, and it should be construed that all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the present application. .

Claims (6)

As a cosmetic composition comprising a tulip extract, saw palmetto extract and nettle extract,
In the cosmetic composition, the weight ratio of the tulip extract, saw palmetto extract, and nettle extract is 1: 0.5 to 2: 0.5 to 2, based on the weight ratio.
To prevent hair loss and promote hair growth,
Cosmetic composition.
delete The method of claim 1,
The cosmetic composition is a tulip composition, saw palmetto extract and nettle extract are each mixed in a weight ratio of 1: 1: 2, cosmetic composition.
delete Cosmetics comprising the cosmetic composition of claim 1.
The method of claim 5,
The cosmetic is one or more selected from the group consisting of shampoo, hair rinse, hair tonic, hair gel, hair lotion, hair pack, hair spray, hair mousse, hair treatment and hair dye.

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