JP2014221739A - Type vii collagen expression promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find a natural product having type VII collagen expression promoting action from among natural products with high safety, and provide a type VII collagen expression promoter comprising such a natural product as an active ingredient.SOLUTION: A type VII collagen expression promoter comprises, as an active ingredient, extracts from one or more plant selected from the group consisting of Foeniculum vulgare, Sophora root, Citrus paradisi, Rubus ellipticus Smith, Prunus persica Batsch, Hypericume rectum and Origanum majorana.

Description

  The present invention relates to a type VII collagen expression promoter.

  The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as collagen, elastin, and hyaluronic acid that are outside these cells and support the skin structure. In young skin, fibroblasts are actively proliferating, and the interaction between skin tissues such as fibroblasts and collagen keeps their homeostasis, ensuring moisture retention, flexibility, elasticity, etc. It is also kept fresh with tension and gloss.

  However, the production of collagen, which is a major component of the extracellular matrix, is affected by certain external factors such as UV irradiation, significant drying of air, excessive skin washing, etc. Causes degradation and alteration. As a result, the moisturizing function and elasticity of the skin are lowered and abnormal exfoliation of the keratin occurs, so that the skin loses its tension and gloss, and exhibits aging symptoms such as rough skin and wrinkles. As described above, changes associated with skin aging, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, and the like are associated with reduction / denaturation of extracellular matrix components such as collagen.

  Among the extracellular matrix components described above, type VII collagen is a major component of anchoring fibers that binds the epidermal basement membrane and the dermis. Type VII collagen is biosynthesized by epidermal keratinocytes and dermal fibroblasts, transported outside the cells, and then forms anchoring fibers. Anchoring fibers are thought to play an important role in strengthening the bond between the epidermal basement membrane and the dermis and maintaining the structure of the skin.

  Here, it is known that the type VII collagen constituting the anchoring fiber is decreased in both physiological aging and photoaging (for example, see Non-Patent Document 1), and the structural change of the skin accompanying this is known. It is thought that it contributes to the appearance of sensations such as wrinkles and sagging, and skin function deterioration due to aging. Therefore, if the production of type VII collagen can be promoted, it is considered that skin aging symptoms such as wrinkles and sagging, and skin function deterioration associated therewith can be prevented and improved.

  Further, when a strong external force or friction is applied to the skin, a wound in which the skin structure itself is damaged, a blister in which the bond between the dermis and the epidermis is broken, or the like is generated. Repair and stabilization of the basement membrane are indispensable for the healing of these, but if the production of type VII collagen can be promoted, it is considered useful for the treatment of such wounds and blisters.

  Conventionally, as a thing which has a VII type collagen production promotion effect, the solvent extract (refer patent document 1), the okra extract (refer patent document 2), etc. of a seed of yuzu are known, for example.

JP 2006-206571 A JP 2009-221110 A

Takuo Tsuji, Journal of the Japanese Society for Skincare, 1995, Vol.105, No.7, p.963-975

  An object of the present invention is to provide a type VII collagen expression promoter that has an action of promoting the expression of type VII collagen from highly safe natural products and uses it as an active ingredient.

  In order to solve the above problems, the type VII collagen expression promoter of the present invention is derived from one or more plants selected from the group consisting of fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum and marjoram. It contains an extract as an active ingredient.

  According to the present invention, it is possible to provide a highly safe VII collagen expression promoter having an excellent VII collagen expression promoting action and high safety.

Embodiments of the present invention will be described below.
The type VII collagen expression promoter of this embodiment contains, as an active ingredient, an extract from one or more plants selected from the group consisting of fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum and marjoram. To do.

  Here, in the present embodiment, the “extract” refers to an extract obtained using the above plant as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or these Either a crude product or a purified product is included.

  Extraction raw materials used in the present embodiment are fennel (scientific name: Foeniculum vulgare), kujin (herbal medicine name), grapefruit (scientific name: Citrus paradisi), Himalayan raspberry (scientific name: Rubus ellipticus Smith), peach (scientific name: Prunus persica Batsch) Hypericum (scientific name: Hypericum erectum, Hypericum perforatum) or marjoram (scientific name: Origanum majorana).

  Fenic (Foeniculum vulgare) is a perennial herb belonging to the genus Felioaceae and is cultivated in India, China, Egypt, etc., and can be easily obtained from these regions. Examples of the constituent parts of fennel that can be used as an extraction raw material include leaves, stems, flower parts, roots, fruit parts, fruit skin parts, fruit core parts, above-ground parts, whole grasses, or mixtures thereof. However, it is preferably a fruit part.

  Kujin (name of crude drug) is the root of Clara (scientific name: Sophora flavescens Aiton), a perennial herb belonging to the genus Clara belonging to the genus Clariaceae distributed throughout Japan, Korea and China, and can be easily obtained from these regions Can do. Kudin is conventionally used as a healthy stomach, diuresis, antipyretic, analgesic and the like.

  Grapefruit (Citrus paradisi) is an evergreen small tree belonging to the genus Citrus mandarin and is cultivated in Shikoku and southern Kyushu, and is easily available from these areas. Examples of the constituent part of grapefruit that can be used as an extraction raw material include a leaf part, a stem part, a flower part, a fruit part, a fruit skin part, a root part, an above-ground part, or a mixture thereof, preferably a fruit part.

  Himalayan Raspberry (Rubus ellipticus Smith) is an evergreen shrub belonging to the genus Rosaceae, and is grown or grown in the Southeast Asia and Southeast China from the Himalayas and can be easily obtained from these regions. The fresh fruit of Himalayan raspberry is fragrant and edible. Examples of the constituent parts of Himalayan raspberry that can be used as an extraction raw material include leaf parts, stem parts, above-ground parts, flower parts, fruit parts, seed parts, root parts, whole plants, or a mixture of these parts. Is the root.

  Peach (Prunus persica Batsch) is a deciduous small tree belonging to the genus Rosaceae, and has been cultivated in China and Japan for a long time and can be easily obtained from these areas. Examples of the constituent parts of the peach that can be used as the extraction part include a leaf part, a stem part, a flower part, a cocoon part, a fruit part, a fruit skin part, a fruit core part, a seed part, a ground part, or a mixture thereof. The leaf portion is preferred.

  Hypericum (Hypericum erectum, Hypericum perforatum) is a perennial herb belonging to the genus Hypericum, Hypericum, and is native to Honshu, Shikoku, Kyushu, etc., and can be easily obtained from these regions. Examples of the constituent part of hypericum that can be used as an extraction raw material include a leaf part, a stem part, a root part, a flower part, an above-ground part, or a mixture thereof, and the above-mentioned part is preferably an above-ground part or whole grass.

  The marjoram (Origanum majorana, also known as: Majorana, oregano) is a perennial herb belonging to the genus Lamiaceae and is distributed in the Mediterranean coast, North Africa, West Asia, etc., and can be easily obtained from these regions it can. Examples of the constituent parts of marjoram that can be used as an extraction raw material include a leaf part, a stem part, a flower part, a bud part, a rhizome part, an above-ground part, or a mixture thereof, and preferably a leaf part.

  The extract from the plant can be obtained by drying the extraction raw material, pulverizing it as it is or using a crusher, and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing a pretreatment such as degreasing, the extraction material can be efficiently extracted with a polar solvent.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in this embodiment includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed solution of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by volume of a lower aliphatic alcohol with respect to 10 parts by volume of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by volume of a lower aliphatic ketone with 10 parts by volume of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by volume of a polyhydric alcohol with respect to the volume part.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dried product is obtained.

  The extract obtained as described above can be used as it is as an active ingredient of the type VII collagen expression promoter, but a concentrated solution or a dried product is easier to use.

  In addition, since the extract from the plant has a peculiar odor, it can be purified for the purpose of decolorization, deodorization and the like within a range not causing a decrease in its physiological activity. Since it is not used in a large amount when it is blended, etc., there is no practical problem even if it is not purified.

  Since the extract from fennel, cucumber, grapefruit, Himalayan raspberry, peach, hypericum or marjoram obtained as described above has an excellent VII collagen expression promoting action, it is effective as a VII collagen expression promoter. It can be used as a component. The type VII collagen expression promoter of this embodiment can be used for a wide range of uses such as pharmaceuticals, quasi drugs, and cosmetics.

  In addition, extracts from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram are used to promote the expression of type VII collagen, and skin aging symptoms such as wrinkles and sagging and the associated skin function You may use as an active ingredient of the prevention or improvement agent of a fall. Furthermore, the extract from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram is used as an active ingredient of a treatment or ameliorating agent for wounds, blisters, etc. using its type VII collagen expression promoting action Also good.

  In the present embodiment, any one of extracts from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram may be used as the active ingredient, or two or more of these may be used. You may mix and use as said active ingredient. When an extract from fennel, cucumber, grapefruit, Himalayan raspberry, peach, hypericum or marjoram is used as the active ingredient, the blending ratio is from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram. What is necessary is just to adjust suitably according to the grade etc. of the type VII collagen expression promotion effect which an extract has.

  The type VII collagen expression promoting agent of the present embodiment may be composed only of an extract from fennel, cucumber, grapefruit, Himalayan raspberry, peach, hypericum or marjoram, or a mixture thereof, or fennel, kujin, grapefruit, and Himalayan raspberry. , Extracts from peaches, hypericum or marjoram or mixtures thereof may be formulated.

  The type VII collagen expression promoter of the present embodiment is a powder, granule, tablet, liquid, etc. according to a conventional method using a pharmaceutically acceptable carrier such as dextrin, cyclodextrin or any other auxiliary agent. It can be formulated into any dosage form. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. The type VII collagen expression promoter can be used by being blended with other compositions (for example, external preparations for skin, cosmetic foods and drinks, etc.), and can be used as an ointment, a liquid for external use, a patch and the like. it can.

  When the type VII collagen expression promoter of this embodiment is formulated, the content of the extract from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram or a mixture thereof is not particularly limited. It can be set as appropriate according to the purpose.

  In addition, the type VII collagen expression promoting agent of the present embodiment may be prepared from, for example, other natural extracts having a type VII collagen expression promoting effect, such as fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram. It can mix | blend with an extract of these, or these mixtures, and can be used as an active ingredient.

  Examples of the method of administering the type VII collagen expression promoter of this embodiment to a patient include transdermal administration, oral administration, and the like. Depending on the type of disease, a suitable method for its prevention / treatment can be selected as appropriate. That's fine.

  In addition, the dose of the type VII collagen expression promoter of this embodiment may be appropriately increased or decreased depending on the type of disease, severity, individual differences among patients, administration method, administration period, and the like.

  The type VII collagen expression promoting agent of the present embodiment is used for the skin aging symptoms such as wrinkles and sagging through the VII type collagen expression promoting action possessed by the extract from fennel, kudin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram. Therefore, it is possible to prevent or improve the decrease in skin function associated therewith. In addition, the type VII collagen expression promoting agent of the present embodiment damaged the skin structure itself through the type VII collagen expression promoting action possessed by the extract from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum or marjoram. It can treat or improve wounds, blisters in which the bond between the dermis and epidermis is broken, and the like. However, the type VII collagen expression promoting agent of the present embodiment can be used for all purposes that are meaningful for exerting the VII type collagen expression promoting action in addition to these uses.

  Moreover, since the VII type | mold collagen expression promoter of this embodiment has the outstanding VII type collagen expression promotion effect | action, it is suitable for mix | blending with a skin external preparation or food-drinks, for example. In this case, an extract from fennel, kujin, grapefruit, himalayan raspberry, peach, hypericum or marjoram or a mixture thereof may be blended as it is, or from fennel, kujin, grapefruit, himalayan raspberry, peach, hypericum or marjoram. A type VII collagen expression promoter formulated from the above extract or a mixture thereof may be blended.

  Here, the topical skin preparation is not limited in its category, and includes a wide range of skin cosmetics, quasi-drugs, pharmaceuticals, and the like used transdermally. Creams, milky lotions, beauty essences, lotions, packs, foundations, lip balms, bath salts, hair nicks, hair lotions, soaps, body shampoos and the like.

  There is no restriction on the category of food and drink, and it includes a wide range of foods such as general foods, health foods, and functional health foods that are taken orally.

  In addition, since the type VII collagen expression promoter of the present embodiment has an excellent type VII collagen expression promoting action, it is related to research related to type VII collagen, for example, the expression control mechanism of VII collagen and the repair mechanism of the basement membrane. It can be suitably used as a reagent for research.

  The type VII collagen expression promoting agent of the present embodiment is suitably applied to humans. However, as long as each effect is exhibited, animals other than humans (for example, mice, rats, hamsters). , Dogs, cats, cows, pigs, monkeys, etc.).

  Hereinafter, although a manufacture example and a test example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all. In the following test examples, lyophilized products (samples 1 to 7) shown in Table 1 were used as test samples.

[Test Example 1] Type VII collagen production promoting action test The plant extract (samples 1 to 7) was tested for the type VII collagen production promoting action as follows.

Normal human epidermal keratinocytes (NHEK) were cultured using normal human neonatal epidermal keratinocyte growth medium (KGM), and then cells were collected by trypsin treatment. The collected cells were diluted with BPE-free KGM medium (KGM-BPE) to a cell density of 1 × 10 ⁵ cells / mL, seeded in a 24-well plate at 500 μL per well, and cultured for 1 day.

  After completion of the culture, the medium is removed, and 500 μL of KGM-BPE medium added with the test sample (samples 1 to 7; see Table 1 below for the sample concentration) or KGM-BPE medium without the sample is added to each well. And cultured for 48 hours. After completion of the culture, the amount of type VII collagen in the medium of each well was measured by ELISA. From the obtained results, the type VII collagen production promotion rate (%) when the sample solution was added was calculated according to the following formula.

VII type collagen production promotion rate (%) = (A / B) × 100
In the formula, A indicates “amount of type VII collagen when the test sample is added”, and B indicates “amount of type VII collagen when no sample is added”.
The results are shown in Table 2.

  As shown in Table 1, samples 1 to 7 all promoted the expression of type VII collagen. Therefore, it was recognized that all extracts from fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum and marjoram have an excellent VII collagen expression promoting effect.

  The type VII collagen expression promoter of the present invention can greatly contribute to the prevention or improvement of skin aging symptoms such as wrinkles and sagging, and the reduction or reduction of skin function associated therewith; the treatment or improvement of wounds, blisters and the like.

Claims (1)

  Type VII collagen production promoter characterized by containing, as an active ingredient, an extract from one or more plants selected from the group consisting of fennel, kujin, grapefruit, Himalayan raspberry, peach, hypericum and marjoram .