KR102212894B1 - Cosmetic Composition For Promoting Hair Volume Containing Walnut Peptides As Active Ingredient - Google Patents

Abstract

The present invention relates to a hair cosmetic composition having a hair volume enhancing effect containing walnut peptide. The hair cosmetic composition for strengthening hair volume has a dermal papilla cell proliferation effect, a cell hair growth signal transduction (β-catenin level, VEGF, IGF-1) effect, a β-catenin expression increase effect, and an effect of enhancing hair tensile strength and hair thickness.

Description

Cosmetic Composition For Promoting Hair Volume Containing Walnut Peptides As Active Ingredient, containing walnut peptide as an active ingredient

The present invention relates to a composition for hair containing a peptide extracted from walnuts as an active ingredient, and more specifically, to a cosmetic composition for hair having an excellent hair volume enhancement effect by containing the walnut peptide.

In general, hair is divided into a hair shaft that extends from the epidermis to the outside and a hair root that is inside the epidermis, and the hair root is again composed of a hair shaft, a dermal papilla, an internal hair root sheath, an external root sheath, and a hair cell. It is composed of water quality. The cuticle of the hair shaft is the outermost layer, occupying about 10 to 15% of the hair, and has the shape of a scale or a tiled roof. While protecting the cortex from external stimuli and inhibiting moisture evaporation, it is easily damaged by mechanical and chemical stimuli. The cortex is located inside the epidermis and occupies about 85 to 90% of the hair and is an important part that determines the quality of the hair. Consisting of keratinous cortex cells, it influences the thickness, elasticity, strength, and texture of the hair, and contains melanin pigments that play a role in the color of the hair. Medulla refers to the hollow area near the center of the hair and is mostly found in thick hair. It contains air in the empty part to maintain body temperature, so it is found more than 50% in the fur of animals living in cold regions.

Meanwhile, 18-methyleicosanoic acid (18-MEA), which protects the epidermis, is present on the outermost surface of the cuticle. It exists as a covalent bond due to a thioester bond with the constituent protein of the hair, and when 18-MEA is lost, the epidermis is damaged by exposing hydrophilic groups such as cysteine residues or nonionic amide groups, which are anions derived from the hair constituent protein. Therefore, it is necessary to supply a hydrophobic fatty acid (Hydrophobic fatty acid) to strengthen the thioester bond and reduce 18-MEA damage.

In general, hair is composed of 80 to 90% protein, and melanin pigment, lipid, moisture, and trace elements. Among them, most of the proteins are made up of keratin, which is a keratin protein containing 14 to 18% cystine among the 18 amino acids that make up it. These keratin proteins are a kind of very strong protein, but can be easily damaged by excessive physicochemical treatment. One of the causes of damage is chemical factors such as perm and dyeing, and can be divided into environmental factors such as aging of the skin or exposure to UV rays, and physical factors such as combing and drying. When damaged by these factors, the hair becomes coarse and loses its gloss, resulting in inferior softness and suppleness, increased friction, making it difficult to care for the hair, and it is impossible to maintain the health of the hair such as the hair is cut or the ends are split. Furthermore, as the hair that has lost its health gradually becomes thinner and loses its elasticity, there is a problem that it is not lifeless and loses the sense of volume while being drooped. Therefore, in order to minimize hair damage, it is effective to reduce keratin protein damage, protect hair, and maintain health by continuously supplying various proteins with amino acid composition similar to keratin protein.

On the other hand, even people with similar hairstyles and hairstyles may have different hair volume depending on the thickness and strength of the hair fibers. For example, if the strength of the hair fibers is weak, the hair protruding from the scalp will lose its support. As a result, the hair is drooped, and when viewed with the naked eye, the hair looks weak, the volume is reduced, or it gives a negative feeling that the hair looks old, so there may be difficulties in hair styling. Therefore, in order to maintain and increase the voluminous feel of the hair, it is important to improve the elasticity of the hair, which can be seen to be improved by enhancing the thickness and strength of the hair through strengthening the hair roots. Accordingly, by promoting the activity of keratinocytes in the hair papilla, it is possible to help the hair elasticity by strengthening the cortex of the hair shaft.

The importance of hair is emerging as one of the various elements expressing beauty to modern people, and consumers' demand for hair-related products is also increasing. However, the health of the hair is deteriorated by ultraviolet rays, fine dust, and physical and chemical stimuli, and a vicious cycle is encountered by using many chemicals to improve the tacky hair volume.

Republic of Korea Patent Publication No. 2014-0117096 discloses an oxidation-type hair dye composition containing a sunscreen agent and natural substances such as marigold and Yugeunpi in a commonly used oxidation-type dye. More specifically, by containing a sunscreen in the existing oxidized hair dye, it effectively inhibits the decomposition of the oxidized dye polymer formed in the cortex of the hair by ultraviolet rays, delaying the color fade in the hair, making the dyed color last longer. You can expect the effect to be maintained.

In Korean Patent Application Publication No. 2019-0038396, a cosmetic composition for hair or scalp that improves the volume of hair is disclosed. More specifically, the volume of hair containing a single hydrophobic polymer that exerts an effect of increasing the volume of hair by increasing the hydrophobicity of the hair shaft region as an active ingredient, or a mixture of a hydrophobic polymer and a nonionic polymer as an active ingredient It relates to a cosmetic composition for promotion.

Korean Patent Registration No. 10-1893194 discloses a cosmetic composition for strengthening hair. The cosmetic composition for strengthening hair may include a wax or fat component; And a peptide component as an active ingredient to form a liquid crystal in the composition, increase the thickness of the hair and give a sense of volume through it, strengthen the strength of the hair and reduce breakage, provide a hair strengthening effect, and improve stability over time.

The inventors of the present invention have studied natural materials that can prevent hair damage and increase volume while helping hair health. As a result, when walnut peptide is added to a cosmetic composition in a specific ratio, hair growth is promoted and the tensile strength of the hair It was confirmed that and the thickness was strengthened to complete the present invention.

The present invention contains an amino acid composition similar to keratin protein, has excellent physiological activity on dermal papilla cells, and can promote hair health and give hair volume by confirming the effect of walnut peptides affecting the change in tensile strength and thickness of hair. It is an object of the present invention to provide a composition for hair containing a peptide.

The above object is achieved by a cosmetic composition for enhancing the volume of hair containing a walnut peptide as an active ingredient.

Preferably, the walnut peptide is characterized in that it contains an amino acid composition similar to that of the hair keratin protein.

In addition, preferably, the cosmetic composition has an effect of proliferating dermal papilla cells, an effect of regulating cell hair proliferation signaling (VEGF, IGF-1), or increasing expression of β-catenin.

Also preferably, the cosmetic composition has an effect of increasing the thickness of the hair or increasing the tensile strength of the hair.

Also preferably, the walnut peptide may be contained in an amount of 0.1 to 30.0% by weight based on the total weight of the cosmetic composition.

The cosmetic composition for hair of the present invention promotes dermal papilla cell proliferation, regulates cell hair proliferation signaling (β-catenin level, VEGF, IGF-1), and has an excellent effect of strengthening the tensile strength and thickness of the hair. It is effective in improving health and hair volume.

1 shows chromatography for amino acid analysis of hair and walnut peptides.
2 is a graph showing the amino acid composition of keratin protein and walnut peptide.
3 is a result of measuring the proliferation rate of human dermal papilla cells of the walnut peptide according to the present invention.
4 is a result of confirming the expression level of the hair growth factor IGF-1 (Insulin-like growth factor) of the walnut peptide according to the present invention.
5 is a result of confirming the expression level of VEGF (Vascular endothelial growth factor), which is a hair growth factor of the walnut peptide according to the present invention.
6 is a result of confirming the expression rate of Wnt/β-catenin of the walnut peptide according to the present invention.
Figure 7 is an electron micrograph of a hair tress before applying a volume spray containing a walnut peptide according to the present invention (Formulation Example 1), Figure 8 is a volume spray containing the walnut peptide according to the present invention (Formulation Example 1) is applied After that, it is an electron micrograph of the hair tress.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings as commonly understood by one of ordinary skill in the relevant fields of the present invention. In addition, although preferred methods or samples are described in the present specification, those similar or equivalent are included in the scope of the present invention.

The term “about” refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or relative to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length. It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless otherwise required by the context, the terms “comprise” and “comprising” include the steps or components presented, or groups of steps or components, but any other step or component, or It is to be understood that a step or group of components is not excluded.

The present invention provides a cosmetic composition for enhancing the volume of hair containing a walnut peptide as an active ingredient.

In the present invention, the meaning of being included as an active ingredient means that the walnut peptide is added to the cosmetic composition for hair to the extent that the cosmetic composition for hair can exhibit the effect of enhancing the volume of hair, and various ingredients for ingredient delivery and stabilization It is meant to include formulating in various forms by adding as an accessory ingredient.

The walnut peptide according to the present invention can be prepared according to a conventional method known in the art, that is, using a conventional proteolytic enzyme under the conditions of a conventional solvent and temperature and pH. Preferably, the walnut peptide is a food grade acidic protease, a neutral protease, or a mixture thereof by adjusting the pH to 5 or less at a temperature of 20 to 60°C in a single or mixed solvent of monohydric alcohol and purified water. It can be manufactured using.

Preferably, it may be prepared including a decomposition process using an enzyme in which papain and pepsin are mixed at a weight ratio of 1:1 to weight by adjusting the pH to 2 to 4 at a temperature of 50 to 55°C in a 100% purified water solvent.

In addition, the walnut peptide of the present invention includes not only the above-described extraction method, but also a substance that has undergone a conventional purification process. Preferably, substances obtained through various purification methods, such as a 1 to tetrahydric alcohol solvent compartment, centrifugation, microfiltration membrane, ultrafiltration membrane separation, and separation by various chromatography are also included in the present invention.

In addition, the walnut peptide of the present invention includes not only the above-described extraction and purification method, but also a material that has undergone a conventional drying process. Preferably, substances obtained through various drying methods such as concentration, hot air drying, spray drying and freeze drying are also included in the present invention.

In addition, the walnut peptide prepared by the above-described manufacturing method of the present invention contains a low molecular weight protein. Preferably, the walnut peptide according to the present invention comprises a substance having a molecular weight range of less than 20 kDa.

The walnut peptide according to the present invention contains an amino acid composition similar to that of keratin protein. Preferably, the walnut peptide contains histidine, lysine, and arginine in a ratio of about 1:3:10 (by weight).

In the cosmetic composition for hair according to the present invention, the walnut peptide exhibits a dermal papilla cell proliferation effect and a hair proliferation signal transduction (VEGF, IGF-1, β-catenin level) enhancing effect.

In addition, the cosmetic composition for hair has an effect of strengthening hair proliferation signal transmission (VEGF, IGF-1, β-catenin level), and strengthening tensile strength and thickness of hair.

According to an embodiment of the present invention, the walnut peptide is preferably contained in an amount of 0.1 to 30.0% by weight based on the total weight of the cosmetic composition for hair.

When the walnut peptide content is less than 0.1% by weight, the effect is insignificant, and when it exceeds 30.0% by weight, the degree of increase in the effect of increasing the content is insignificant, there is a problem in safety and stability of the formulation, and is not economical.

On the other hand, the cosmetic composition for hair of the present invention may be prepared in any formulation conventionally prepared in the art, for example, it may be formulated as a curl cream, hair gel, volume spray, shampoo, treatment, etc. It is not limited.

On the other hand, the cosmetic composition for hair of the present invention can be used in duplicate with other cosmetic compositions other than the present invention. In addition, the composition for hair according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to the condition or taste of the user's scalp.

The cosmetic composition for hair containing the walnut peptide according to the present invention has excellent dermal papilla cell proliferation effect and hair proliferation signal transduction (VEGF, IGF-1, β-catenin level) reinforcing effect, and thus hair tensile strength And the thickness improvement effect. In addition, since it uses natural materials, it is harmless to the human body and has excellent safety.

In addition, components commonly used in cosmetic compositions for hair, that is, antioxidants, metal sequestering agents, solvents, surfactants, thickeners, fragrances, and conditioning agents, etc., may be used within the range that does not impair the effects of the present invention. .

The cosmetic composition for hair containing the walnut peptide of the present invention as an active ingredient may be applied as a shampoo, conditioner, treatment, hair pack or essence formulation.

Hereinafter, the present invention will be specifically described with reference to examples, but the scope of the present invention is not limited by these examples.

Example 1: Preparation of walnut peptide

The walnut peptide powder was prepared by the following method.

(1) Pulverization of walnut: The outer shell of walnut is removed, and walnut powder is obtained using a crushing and crushing machine.

(2) Enzymatic decomposition of protein: The walnut powder is immersed in purified water to maintain a temperature of 50 to 55°C, and an enzyme mixed with papain and pepsin in a ratio of 1:1 (weight ratio) by adjusting the pH to 2 to 4 is walnut powder. Add 0.5 to 2.0% by weight based on the weight. The mixture was stirred for 6 h to undergo an enzymatic decomposition reaction, and boiled for 30 min to remove activity, and then centrifuged to obtain a supernatant.

(3) Separation and purification: 1:1 ethanol by weight is added to the supernatant and allowed to stand at a low temperature, and then the separated supernatant is separately centrifuged to remove the precipitate and the supernatant is separated.

(4) Filtration: The filtrate is obtained by passing the separated liquid through a fine filtration membrane of 0.1 to 0.5 μm.

(5) Drying: Freeze-drying the filtrate at -40℃ A walnut peptide powder with a molecular weight of less than 20 kDa is obtained.

Experimental Example 1: Amino Acid Analysis

Amino acid analysis using HPLC was performed to confirm the association between the keratin protein, which is a major component of hair, and the constituent amino acid composition of the walnut peptide obtained in Example 1.

(1) Pretreatment of sample: Take an appropriate amount from hair tress, add 6N HCl, hydrolyze at 120℃ for 24 hours, dilute with purified water, and filter with a 0.45 μm syringe filter. Also, take an appropriate amount of the walnut peptide of Example 1, add 6N HCl, hydrolyze at 120° C. for 24 hours, dilute with purified water, and filter with a 0.45 μm syringe filter.

(2) Analysis conditions

-Column: Cation column

-Detector: UV/Vis detector 340 nm, 450 nm/Fluorescence 266 nm, 305 nm

-Mobile Phase: 40mM Sodiumphosphate(Di basic) 0.1% Phosphoric acid(A)

Acetonitrile/Methanol/DW=45/45/10(B)

-Flow: 1.5 ml/min

-Temperature: 40℃

Time A B 0.0 100 0 1.9 100 0 18.1 43 57 18.6 20 80 22.3 20 80 23.2 100 0 26.0 100 0

Chromatography for amino acid analysis of hair and walnut peptides is shown in Fig. 1, and the amino acid composition of keratin proteins and walnut peptides is shown in Fig. 2. The content of cysteine, the main amino acid of the keratin protein, is about 14.5% by weight in the walnut peptide, and the composition ratio of the histidine, lysine, and arginine of the hair keratin protein is 1:3:10 (weight ratio). Since the ratio of lysine and arginine was about 1:3:10, it was confirmed that the constituent amino acids were similar. Since cysteine, a major component of keratin protein, is high, it can compensate for the cysteine bonds broken by physical and chemical stimulation, and helps to minimize cuticle damage.

Experimental Example 2: Evaluation of dermal papilla cell proliferation

Human follicle dermal papilla cells (HFDPC) were dispensed in a 96 well plate at 1 × 10 ⁴ cells/ml, and stabilized in a cell incubator at 37° C. and 5% CO ₂ for 24 hours. After replacing this with DMEM medium containing 1% FBS, the samples were diluted by concentration, treated, and reacted for 48 hours. Then, 20 µl of MTT reagent at a concentration of 5 mg/ml was added to each well and reacted in a cell incubator at 37° C. and 5% CO ₂ for 2 to 3 hours. The supernatant of each well after the reaction was completely removed, and 100 µl of DMSO was added to completely lyse the cells, and the absorbance was measured at 550 nm. At this time, as a positive control, minoxidil, a hair growth promoting agent, was used, and the cell proliferation rate of each sample group was calculated as compared with the absorbance value of the control group and shown in FIG. 3.

As shown in FIG. 3, it was confirmed that the dermal papilla cell proliferation rate increased by about 25% in the group treated with the walnut peptide at a concentration of 100 ug/ml.

Experimental Example 3: Hair Growth Factor Expression Promotion Effect

The dermal papilla cells were dispensed into 96 wells at a concentration of 1 × 10 ⁵ cells/ml and incubated for one day in a 37°C, 5% CO ₂ incubator, and the samples were treated by concentration and reacted in a 37°C, 5% CO ₂ incubator for 24 hours. . It was measured by enzyme-linked immunosorbent assay (ELISA) using the culture medium of the cells. The expression of IGF-1 (Insuline-like growth factor) and VEGF (Vascular endothelial growth factor) in the cell culture was analyzed using an ELISA kit, and absorbance was taken at 450 nm to show the amount of each produced in FIGS. 4 and 5, respectively. Done. At this time, as a positive control, minoxidil, a hair growth promoting agent, was used.

4 and 5, in the group treated with the walnut peptide at a concentration of 100 ug/ml, the amount of IGF-1 production increased by about 30% and the amount of VEGF production increased by about 43%.

Example 2: Preparation of hair products using walnut peptide

A composition for hair containing the walnut peptide was prepared according to a conventional method with the composition and content in the following table.

Formulation Example 1: Volume Spray

A volume spray was prepared according to a conventional method with the components and contents shown in Table 2 below.

no. Raw material name Content (% by weight) A B C D One Modified alcohol to. 100 to. 100 to. 100 to. 100 2 Purified water 20.000 20.000 20.000 20.000 3 Walnut Peptide (Example 1) 1.000 1.000 1.000 1.000 4 Acrylate/Octyl Acrylamide Copolymer 3.000 - - - 5 PvP - 3.000 - - 6 VP/VA Copolymer - - 3.000 - 7 Octylacrylamide/acrylate/butylaminoethylmethacrylate copolymer - - - 3.500 8 Aminomethylpropanol Appropriate amount Appropriate amount Appropriate amount Appropriate amount 9 Spices Appropriate amount Appropriate amount Appropriate amount Appropriate amount

Formulation Example 2: Aerosol Spray

An aerosol spray was prepared according to a conventional method with the components and contents shown in Table 3 below.

no. Raw material name Content (% by weight) A B One Modified alcohol to. 100 to. 100 2 Walnut Peptide (Example 1) 1.000 1.000 3 LPG (P-30%) Appropriate amount Appropriate amount 4 Acrylate/Octyl Acrylamide Copolymer 5.000 - 5 Octylacrylamide/acrylate/butylaminoethylmethacrylate copolymer - 5.000 6 Aminomethylpropanol Appropriate amount Appropriate amount 7 Spices Appropriate amount Appropriate amount

Formulation Example 3: Curl Cream

A curl cream for hair with the components and contents shown in Table 4 below was prepared according to a conventional method.

no. Raw material name Content (% by weight) A B One Purified water to. 100 to. 100 2 Walnut Peptide (Example 1) 1.000 1.000 3 glycerin 5.000 5.000 4 Purified water, cetrimonium chloride 1.000 1.000 5 Stearyl alcohol 4.000 4.000 6 Cetyl alcohol 4.000 4.000 7 Ceteares-30 3.000 3.000 8 Glyceryl stearate 1.000 1.000 9 Isopropyl Myristate 3.500 3.500 10 Phenoxyethanol 0.500 0.500 11 Dimethicone 5.000 5.000 12 Aminomethylpropanol Appropriate amount Appropriate amount 13 VP/methacrylamide/vinylimidazole copolymer 1.000 1.000 14 PvP 3.000 - 15 VP/VA Copolymer - 3.000

Formulation Example 4: Hair Gel

A hair gel was prepared according to a conventional method with the components and contents shown in Table 5 below.

no. Raw material name Content (% by weight) A B C One Purified water to. 100 to. 100 to. 100 2 Walnut Peptide (Example 1) 1.000 1.000 1.000 3 Carbomer 1.000 1.000 1.000 4 Triethanolamine Appropriate amount Appropriate amount Appropriate amount 5 Spices Appropriate amount Appropriate amount Appropriate amount 6 Modified alcohol 10.000 10.000 10.000 7 PvP 5.000 - - 8 VP/VA Copolymer - 5.000 - 9 Maltitol - - 5.000

Formulation Example 5: Shampoo

A shampoo for hair was prepared according to a conventional method with the components and contents shown in Table 6 below.

no. Raw material name Content (% by weight) One Purified water to. 100 2 Walnut Peptide (Example 1) 1.000 3 Disodium EDTA 0.030 4 Polyquaternium-10 0.100 5 Ammonium lauryl sulfate 40.500 6 Ammonium laureth sulfate 15.500 7 Cocamidopropylbetaine 6.000 8 Propylene glycol 2.000 9 Sodium xylenesulfonate 2.000 10 Glycol distearate 3.000 11 Cocamide D.A. 1.000 12 Lauramide D.A. 1.000 13 Salicylic acid 0.130 14 Simethicone 3.000 15 antiseptic Appropriate amount 16 incense Appropriate amount

Formulation Example 6: Conditioner and Treatment

Conditioners and treatments for hair with the components and contents shown in Table 7 below were prepared according to a conventional method.

no. Ingredients Content (% by weight) One Purified water to. 100 2 Walnut Peptide (Example 1) 1.00 3 Disodium EDTA 0.02 4 Hydroxyethyl cellulose 0.20 5 Panthenol 0.05 6 Propylene glycol 1.50 7 glycerin 2.00 8 Cetyl alcohol 3.50 9 Stearyl alcohol 1.50 10 Glyceryl Stearate SE 0.50 11 Dimethicone 1.50 12 antiseptic Appropriate amount 13 incense Appropriate amount

Formulation Example 7: Hair Pack

A hair pack was prepared according to a conventional method with the components and contents shown in Table 8 below.

no. Ingredients Content (% by weight) One Purified water to. 100 2 Walnut Peptide (Example 1) 1.00 3 Disodium EDTA 0.05 4 Panthenol 0.10 5 glycerin 5.00 6 Carbomer 0.60 7 Hydroxyethyl cellulose 0.30 8 ethanol 7.00 9 Polyoxyethylene hardened castor oil 1.00 10 Triethanolamine 0.60 11 Polyvinylpyrrolidone 5.00 12 antiseptic Appropriate amount 13 incense Appropriate amount

Experimental Example 4: Wnt/β-catenin expression rate confirmation

The dermal papilla cells were dispensed into 6 wells at a concentration of 2 × 10 ⁵ cells/well and incubated for one day in a 37°C, 5% CO ₂ incubator, and the samples were treated by concentration and cultured for 24 hours. The cultured cells were collected, lysed, and protein was quantified using BCA assay. The quantified protein was electrophoresed on a 10% SDS-PAGE gel and transferred to a PVDF membrane. After shaking treatment with the primary antibody (β-catenin, santa cruz, USA) overnight at 4°C, the HRP-conjugated secondary antibody was reacted at room temperature for 2 hours. Then, it was washed 3 times with 1 × TBST and the band was confirmed using an ECL reaction kit. The intensity of each band was quantified based on the expression of β-actin and the expression rate of β-catenin was calculated, and the positive control was shown in FIG. 6 using minoxidil, a hair growth promoting agent.

As shown in FIG. 6, β-catenin expression increased by about 121% in the group treated with the walnut peptide at a concentration of 100 ug/ml, and 107% in the group treated with the 3% concentration of Formulation Example 1 including the walnut peptide. Increased.

Experimental Example 5: Hair diameter measurement

The hair tress was fixed on a slide glass and the average diameter was measured with an electron microscope. Further, the volume spray of Formulation Example 1 was applied to the hair tress, treated for 60 minutes, washed with purified water, dried, and then the average diameter of the hair strands was measured in the same manner as described above.

The photographs measuring the thickness of each hair tress are shown in FIGS. 7 and 8. In addition, using the measured data, the hair diameter increase rate was calculated from the following equation and shown in Table 9.

Hair diameter increase rate (%) = {(TT ₁ )/T ₁ }*100 (%)

T: hair diameter after treatment with the main component material; T ₁ : normal hair diameter

Normal hair thickness Treatment hair thickness including main ingredient Hair diameter increase rate 0.053 mm 0.068 mm 28.3%

Experimental Example 6: Measurement of hair tensile strength

The hair tress was measured using a tensile strength measuring device (Diastron Generic Tensile Tester) under conditions of a clamping distance of 20 mm and a tensile speed of 20 mm/min.

After applying the volume spray of Formulation Example 1 to the hair tress, treating it for 60 minutes, washing it with purified water, drying it, and then using a tensile strength measuring device (Diastron Generic Tensile Tester) under the conditions of a clamping distance of 20 mm and a tensile speed of 20 mm/min. Measured.

Using the measured data, the hair tensile strength increase rate was calculated from the following equation and shown in Table 10.

Hair tensile strength increase rate (%) = {(SS ₁ )/S ₁ }*100 (%)

S: hair tensile strength after treatment with the main component material; S ₁ : normal hair tensile strength

Normal hair tensile strength Main ingredient treatment hair tensile strength Hair tensile strength increase rate 42.5 gmf 44.8 gmf 5.4%

Claims (5)

A walnut peptide containing histidine, lysine and arginine in a weight ratio of 1:3:10 is contained as an active ingredient, and the effect of dermal papillary cell proliferation, the effect of regulating cell hair proliferation signaling (VEGF, IGF-1), or β-catenin ( β-catenin), characterized in that it has an effect of increasing expression, a cosmetic composition for enhancing the volume of hair. The cosmetic composition of claim 1, wherein the walnut peptide contains an amino acid composition similar to that of a hair keratin protein. The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of increasing hair thickness or increasing hair tensile strength. The cosmetic composition according to claim 1, wherein the walnut peptide is contained in an amount of 0.1 to 30.0% by weight based on the total weight of the cosmetic composition. delete

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