KR102376630B1 - Cosmetic composition for improving hair loss or stimulating hair growth comprising cell growth stimulants as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating hair loss or promoting hair growth, comprising a cell growth promoting substance as an active component, wherein the cell growth promoting substance of the present invention is CM50 comprising: 1-110 ng/㎖ of an epidermal growth factor (EGF); 18-110 ng/㎖ of a basic fibroblast growth factor (bFGF); 3-50 mM of ascorbic acid; and 90-110 mM of N-acetyl-L-glutamine (NAG). The CM50 has excellent effects of increasing the proliferation ability and collagen synthesis ability of dermal papilla cells and alleviating hair loss of a patient who suffers from hair loss, thereby being usefully used as a composition for alleviating hair loss or promoting hair growth without side effects.

Description

A cosmetic composition for improving hair loss or stimulating hair growth containing a cell growth promoting substance as an active ingredient {Cosmetic composition for improving hair loss or stimulating hair growth comprising cell growth stimulants as effective component}

The present invention relates to a cosmetic composition for improving hair loss or promoting hair growth containing a cell growth promoting substance as an active ingredient.

Hair is generated from dermal papilla cells in hair follicles, and there are about 3,000 dermal papilla cells in normal hair follicles. On average, 50 to 70 hairs fall out per day and grow and maintain periodically. Due to environmental pollution, stress, hormonal imbalance, scalp disease, aging, etc., hair loss is promoted and the number of patients with hair loss who cannot grow hair is increasing rapidly. It is said that there are about 10 million people with hair loss in Korea and about 250 million in China. Drugs approved by the FDA for hair loss treatment include minoxidil and finasteride, which are effective in improving hair loss. It's becoming a problem.

On the other hand, exosomes or extracellular vesicles secreted during the culture and proliferation of dermal papilla cells contain many cell growth promoting substances. These substances are known to play important roles in cell development, division, activity, long-term growth, and intercellular substance exchange. In particular, cell growth promoting substances that affect the proliferation and division of dermal papilla cells include epidermal growth factor (EGF), insulin like growth factor-1 (IGF-1), keratin Cell growth factor (keratinocyte growth factor, KGF), platelet derived growth factor (PDGF), vascular endothelial growth factor (vascular endothelial cell growth factor, VEGF), fibroblast growth factor (fibroblast growth factor, FGF), etc. There is this.

Meanwhile, Korean Patent No. 2031931 discloses 'a composition for improving hair loss containing gold nanoparticles/saponin complex as an active ingredient and a method for manufacturing the same', and Korean Patent No. 2013803 includes 'a novel compound derived from chestnuts' 'A composition for improving hair loss is disclosed, but there is no description of a cosmetic composition for improving hair loss or promoting hair growth containing the cell growth promoting material of the present invention as an active ingredient.

The present invention has been derived by the above requirements, and the present inventors have prepared 2 ng/ml of epidermal growth factor (EGF) and 100 ng/ml of basic fibroblast growth factor (bFGF). ), 40 mM ascorbic acid and 100 mM N-acetyl-L-glutamine (NAG) were prepared to prepare CM50, and the proliferative capacity of dermal papilla cells by treatment of the CM50 And it was confirmed that the collagen synthesis ability was significantly increased compared to the control group. In addition, as a result of manufacturing a cosmetic containing CM50 and conducting a clinical test for hair loss patients, it was confirmed that hair loss was improved and hair growth was promoted, thereby completing the present invention.

In order to solve the above problems, the present invention provides a cosmetic composition for improving hair loss or promoting hair growth containing CM50 as an active ingredient.

In addition, the present invention provides a skin external preparation for improving hair loss or promoting hair growth, containing CM50 as an active ingredient.

In addition, the present invention provides a medium composition for culturing dermal papilla cells with increased proliferation and collagen synthesis, containing CM50 as an active ingredient.

CM50 according to the present invention is expected to be useful as a composition for improving hair loss or promoting hair growth without side effects because it has excellent effects of increasing the proliferative capacity and collagen synthesis of dermal papilla cells and of improving hair loss in patients with hair loss.

1 is a result of analyzing the growth rate of dermal papilla cells by treatment with a cell growth promoting substance by concentration, A is the result of treatment with epidermal growth factor (EGF), B is the basic fibroblast growth factor (basic) fibroblast growth factor, bFGF) treatment result, C is the result of treatment with ascorbic acid, D is the result of treatment with N-acetyl-L-glutamine (NAG) am. Control is the control group without any treatment.
2 is a result of analyzing the proliferation rate of dermal papilla cells by CM50 treatment of the present invention. Control is the control group without any treatment.
3 is a result of analyzing the collagen synthesis ability of dermal papilla cells by the CM50 treatment of the present invention. Control is the control group without any treatment.
4 is a photograph showing the results of a clinical test performed on a hair loss patient using a cosmetic containing CM50 of the present invention.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for improving hair loss or promoting hair growth containing CM50 as an active ingredient.

In the cosmetic composition for improving hair loss or promoting hair growth of the present invention, the CM50 is preferably 1 to 110 ng/ml of epidermal growth factor (EGF), and 18 to 110 ng/ml of basic fibroblast growth. It may be composed of a factor (basic fibroblast growth factor, bFGF), 3-50 mM ascorbic acid, and 90-110 mM N-acetyl-L-glutamine (NAG), , more preferably 1-3 ng/ml of epidermal growth factor (EGF), 90-110 ng/ml of basic fibroblast growth factor (bFGF), 30-50 mM It may consist of ascorbic acid and 90 to 110 mM of N-acetyl-L-glutamine (NAG), most preferably 2 ng/ml of epidermal growth factor; It may consist of 100 ng/ml of basic fibroblast growth factor, 40 mM ascorbic acid, and 100 mM N-acetyl-L-glutamine, but is not limited thereto.

In addition, the CM50 is characterized by increasing the proliferative capacity and collagen synthesis ability of dermal papilla cells.

In the cosmetic composition for improving hair loss or promoting hair growth of the present invention, the CM50 may be included in an amount of 1 to 10% by weight based on the total weight of the composition, but is not limited thereto.

The cosmetic composition for improving hair loss or promoting hair growth of the present invention can be used for preventing, treating or improving alopecia, wherein the alopecia is alopecia areata, hereditary androgenetic alopecia, telogen effluvium, Among traumatic alopecia, Trichotillomania, Pressure alopecia, genital alopecia, alopecia pityroides, Alopecia syphilitica, Alopecia seborrheica, symptomatic alopecia, alopecia scar or congenital alopecia can be selected.

In the cosmetic composition according to an embodiment of the present invention, the cosmetic composition for improving hair loss or promoting hair growth is a cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel , Skin Lotion, Skin Softener, Skin Toner, Astringent, Milk Lotion, Moisture Lotion, Nourishing Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Nourishing Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing It may have any one formulation selected from the group consisting of cream, body lotion, and body cleanser, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol fatty esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyl taurate, sarcosinate as carrier components , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The cosmetic composition of the present invention may further contain excipients including fungicides, hydrotropes, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.

The present invention also provides an external preparation for skin for improving hair loss or promoting hair growth, containing CM50 as an active ingredient.

The external preparation for skin may be prepared in the form of a cream, gel, patch, ointment, warning agent, lotion, liquid, emulsion, powder or spray, but is not limited thereto.

The present invention also provides a method for preventing or treating hair loss, comprising the step of applying the cosmetic composition to the hair loss site.

In the method for preventing or treating hair loss according to the present invention, the subject to which the cosmetic composition is applied is not particularly limited as long as it is an individual for the purpose of preventing or treating hair loss. For example, non-human animals such as monkey, dog, cat, rabbit, guinea pig, rat, mouse, cow, sheep, pig, goat, etc. can be applied to any subject, such as humans, and the amount or number of times the cosmetic composition is applied depends on the individual type. And it may be determined according to severity, age, sex, sensitivity, treatment period, etc., but is not limited thereto.

The present invention also provides a medium composition for culturing dermal papilla cells with increased proliferation and collagen synthesis, containing CM50 as an active ingredient.

In the medium composition according to the present invention, the CM50 is the same as described above.

In the medium composition according to an embodiment of the present invention, the CM50 may be added to various commercially available media known for dermal papilla cell culture, but is not limited thereto.

In addition, when the medium composition containing the CM50 as an active ingredient is used for dermal papilla cell culture, the proliferative capacity and collagen synthesis ability of dermal papilla cells can be increased, and the collagen of dermal papilla cells cultured in three dimensions rather than two-dimensionally cultured dermal papilla cells. It is characterized by further increasing the synthesis ability.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited thereto.

Example 1. Evaluation of the optimal concentration of a cell growth promoting substance

An assay using CCK (cholecystokinin) was performed to evaluate the optimal concentration of a cell growth promoting substance that increases the growth rate of dermal papilla cells. 1.5 × 10 ⁴ cells/well of dermal papilla cells were placed in a 24-well plate and cultured in DMEM/F12 (Dulbecco's Modified Eagle Medium Nutrient Mixture/F-12) medium, 37°C, 5% CO ₂ conditions for 24 hours. Then, the medium was removed and replaced with DMEM/F12 medium containing 2% fetal bovine serum (FBS), and starvation was performed again for 24 hours. Then, 2-100 ng/ml of epidermal growth factor (EGF), 2-100 ng/ml of basic fibroblast growth factor (bFGF), 4-100 mM of ascorbic acid ( ascorbic acid) or 4-100 mM N-acetyl-L-glutamine (N-acetyl-L-glutamine, NAG), respectively, and cultured for 48 hours. Thereafter, the medium was removed, and 200 μl of 5 mg/ml CCK was treated per well and reacted for 2 hours. The CCK reagent was removed while taking care not to disturb the formed formazan crystals, was added to dissolve the formazan crystals. Then, 100 μl was recovered from each well, transferred to a 96-well plate, and absorbance was measured at 540 nm wavelength with a microplate reader. The number of cells in the untreated control group was 100%, and the dermal papilla cell proliferation rate (%) of each treatment group was shown.

As a result, it was confirmed that the proliferation rate of dermal papilla cells in the epidermal growth factor-treated group of 2-100 ng/ml was significantly increased compared to the control group that was not treated with anything. In addition, it was confirmed that the growth rate of dermal papilla cells in the 20-100 ng/ml basic fibroblast growth factor-treated group was significantly increased compared to the control group that was not treated with anything, and when the basic fibroblast growth factor treatment of less than 20 ng/ml was treated, It was confirmed that the proliferation rate of dermal papilla cells did not increase. In addition, it was confirmed that the growth rate of dermal papilla cells in the group treated with 4-40 mM ascorbic acid was significantly increased compared to the control group not treated with anything, and it was confirmed that the growth rate of dermal papilla cells was decreased by treatment with more than 40 mM ascorbic acid. (Fig. 1).

Example 2. Analysis of dermal papilla cell proliferation rate of CM50

Based on the results of Example 1, 2 ng/ml of epidermal growth factor, 100 ng/ml of basic fibroblast growth factor, 40 mM of ascorbic acid, and 100 mM of N-acetyl-L-glutamine were mixed. CM50 was prepared, and after adding to the medium so that the final concentration of each cell growth promoting substance was as described above, the dermal papilla cell proliferation rate was measured in the same manner as described in Example 1 above.

As a result, it was confirmed that the proliferation rate of dermal papilla cells in the CM50 treatment group significantly increased compared to the control group not treated with anything (FIG. 2).

Example 3. Analysis of dermal papilla cell collagen synthesis ability of CM50

After treatment with CM50 while culturing dermal papilla cells in two or three dimensions, the collagen content was measured. Specifically, 1.5 × 10 ⁴ cells/well of dermal papilla cells were put in a 24-well plate and DMEM/ containing CM50, 100 units/ml penicillin, 100 units/ml streptomycin and 0.25 μg/ml amphotericin B Using F12 medium, it was cultured in two dimensions at 37° C., 5% CO ₂ condition. In addition, in a 24-well plate, dermal papilla cells were placed in DMEM/F12 medium containing CM50, 100 units/ml penicillin, 100 units/ml streptomycin and 0.25 μg/ml amphotericin B, and the diameter of 150 according to the number of cells. After putting ~350 μm gelatin microcarrier at 0.1~1.0 g per 100 ml of culture medium, 37℃, 5% CO ₂ , It was cultured in three dimensions at 20-50 rpm.

After culturing the medium of the two-dimensionally cultured dermal papilla cells or the three-dimensionally cultured dermal papilla cells for 72 hours, the medium was recovered and the collagen synthesized and secreted by the dermal papilla cells using a collagen assay kit (Sircol ^TM collagen assay kit). The content was measured.

As a result, it was confirmed that the collagen content of dermal papilla cells in the CM50 treatment group increased significantly compared to the control group that was not treated with anything. It was confirmed that the content increased by about 188% (FIG. 3).

Based on the above results, when CM50 according to the present invention is treated with dermal papilla cells cultured in three dimensions using a biocompatible microcarrier, collagen synthesis ability is remarkably increased, making hair stronger and preventing hair loss. was found to be

Example 4. Clinical trial using cosmetics containing CM50

Essence-type cosmetics (Table 1) containing CM50 of the present invention were prepared and applied to hair loss patients between 39 and 60 years of age once or twice a day for 6 months, and pictures were taken on a set date each month. .

Composition of cosmetics containing CM50 designation purpose content(%) CM50 active ingredient 1-4 pentylene glycol




skin conditioning agent 0.06~0.07 sodium phosphate 0.004~0.005 lecithin 0.004~0.005 Phenoxyethanol 0.006~0.007 glycerin 0.01~0.02 Disodium EDTA 0.05~0.06 glycine soja (soybean) oil 0.01~0.02 Hydrogenated phosphatidylcholine
(hydrogenated phosphatidylcholine) 0.005-0.006 sodium oleate 0.007~0.008 Purified water - up to 100

As a result, it was confirmed that cosmetics containing CM50 had the effect of improving hair loss and promoting hair growth in hair loss patients (FIG. 4).

Claims (6)

1-110 ng/ml of epidermal growth factor (EGF), 18-110 ng/ml of basic fibroblast growth factor (bFGF), 3-50 mM of ascorbic acid ) and 90 to 110 mM of N-acetyl-L-glutamine (N-acetyl-L-glutamine, NAG), a cosmetic composition for improving hair loss or promoting hair growth containing CM50 as an active ingredient. delete The cosmetic composition for improving hair loss or promoting hair growth according to claim 1, wherein the CM50 increases the proliferative ability and collagen synthesis ability of dermal papilla cells. 1-110 ng/ml of epidermal growth factor (EGF), 18-110 ng/ml of basic fibroblast growth factor (bFGF), 3-50 mM of ascorbic acid ) and 90 to 110 mM of N-acetyl-L-glutamine (N-acetyl-L-glutamine, NAG) containing CM50 as an active ingredient, an external preparation for hair loss improvement or hair growth promotion. [Claim 5] The skin external preparation for improving hair loss or promoting hair growth according to claim 4, wherein the external preparation for skin is prepared in the form of a cream, gel, patch, ointment, warning agent, lotion, liquid, emulsion, powder or spray. . 1-110 ng/ml of epidermal growth factor (EGF), 18-110 ng/ml of basic fibroblast growth factor (bFGF), 3-50 mM of ascorbic acid ) and 90 to 110 mM of N-acetyl-L-glutamine (N-acetyl-L-glutamine, NAG) containing CM50 as an active ingredient, a medium composition for culturing dermal papilla cells with increased proliferation and collagen synthesis.

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