WO2023120900A1 - Cosmetic composition for ameliorating hair loss or promoting hair growth comprising cell growth promoter as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for ameliorating hair loss or promoting hair growth, comprising a cell growth promoter as an active ingredient. The cell growth promoter as the active ingredient of the present invention is CM50 consisting of 1-110 ng/ml of epidermal growth factor (EGF), 18-110 ng/ml of basic fibroblast growth factor (bFGF), 3-50 mM of ascorbic acid, and 90-110 mM of N-acetyl-L-glutamine (NAG), having an excellent effect of increasing the proliferation ability of dermal papilla cells and collagen synthesis performance and ameliorating hair loss in a patient having hair loss, and thus it is expected that the cell growth promoter could be usefully utilized in a composition for improving hair loss and promoting hair growth without side effects.

Description

Cosmetic composition for improving hair loss or promoting hair growth containing a cell growth promoting substance as an active ingredient

The present invention relates to a cosmetic composition for improving hair loss or promoting hair growth containing a cell growth promoting substance as an active ingredient.

Hair is generated from dermal papilla cells in hair follicles, and there are about 3,000 dermal papilla cells in normal hair follicles. An average of 50 to 70 hairs fall out per day, and they grow and are maintained periodically. Due to environmental pollution, stress, hormonal imbalance, scalp disease, and aging, hair loss is promoted and hair loss patients who do not grow hair are rapidly increasing. It is said that there are about 10 million patients with hair loss in Korea and about 250 million in China. Drugs approved by the FDA as hair loss treatments include minoxidil and finasteride, which have excellent hair loss improvement effects, but side effects such as general hair growth, decreased sexual function, muscle loss, and circulation disorders appear when used for a long time. It's becoming a problem.

On the other hand, exosomes or extracellular vesicles secreted in the process of culturing and proliferating dermal papilla cells contain many cell growth promoting substances. These substances are known to play important roles in cell development, division, activity, long-term growth and exchange of substances between cells. In particular, cell growth-promoting substances that affect the proliferation and division of dermal papilla cells include epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), and keratin. Cell growth factor (KGF), platelet derived growth factor (PDGF), vascular endothelial cell growth factor (VEGF), fibroblast growth factor (FGF), etc. there is

Meanwhile, Korean Patent No. 2031931 discloses 'a composition for improving hair loss containing a gold nanoparticle/saponin complex as an active ingredient and a method for preparing the same', and Korean Patent No. 2013803 discloses 'a novel compound derived from chestnut burdock. Although a 'composition for improving hair loss' is disclosed, there is no description of a cosmetic composition for improving hair loss or promoting hair growth containing the cell growth promoting substance of the present invention as an active ingredient.

The present invention was derived from the above needs, and the present inventors found that 2 ng/ml of epidermal growth factor (EGF) and 100 ng/ml of basic fibroblast growth factor (bFGF) ), CM50 consisting of 40 mM ascorbic acid and 100 mM N-acetyl-L-glutamine (NAG) was prepared, and the proliferative ability of dermal papilla cells by treatment of the CM50 And it was confirmed that the collagen synthesis ability was significantly increased compared to the control group. In addition, as a result of preparing cosmetics containing the CM50 and conducting a clinical test on hair loss patients, it was confirmed that hair loss was improved and hair growth was promoted, thereby completing the present invention.

In order to solve the above problems, the present invention provides a cosmetic composition for improving hair loss or promoting hair growth containing CM50 as an active ingredient.

In addition, the present invention provides an external skin preparation for improving hair loss or promoting hair growth, containing CM50 as an active ingredient.

In addition, the present invention provides a medium composition for culturing dermal papilla cells with increased proliferative ability and collagen synthesis ability, containing CM50 as an active ingredient.

Since CM50 according to the present invention has an excellent effect of increasing the proliferative ability and collagen synthesis ability of dermal papilla cells and improving the hair loss of hair loss patients, it is expected to be useful as a composition for improving hair loss or promoting hair growth without side effects.

1 is a result of analyzing the proliferation rate of dermal papilla cells by treatment with cell growth promoting substances by concentration, A is the result of treatment with epidermal growth factor (EGF), and B is basic fibroblast growth factor (basic fibroblast growth factor). fibroblast growth factor (bFGF) treatment result, C is ascorbic acid treatment result, D is N-acetyl-L-glutamine (NAG) treatment result am. Control is a control group that is not treated with anything.

Figure 2 is the result of analyzing the proliferation rate of dermal papilla cells by the CM50 treatment of the present invention. Control is a control group that is not treated with anything.

Figure 3 is the result of analyzing the collagen synthesis ability of dermal papilla cells by the CM50 treatment of the present invention. Control is a control group that is not treated with anything.

Figure 4 is a photograph showing the results of a clinical test conducted on hair loss patients using cosmetics containing CM50 of the invention.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for improving hair loss or promoting hair growth containing CM50 as an active ingredient.

In the cosmetic composition for improving hair loss or promoting hair growth of the present invention, the CM50 is preferably 1 to 110 ng/ml of epidermal growth factor (EGF) and 18 to 110 ng/ml of basic fibroblast growth It may consist of basic fibroblast growth factor (bFGF), 3 to 50 mM of ascorbic acid and 90 to 110 mM of N-acetyl-L-glutamine (NAG), , More preferably, 1 to 3 ng/ml of epidermal growth factor (EGF), 90 to 110 ng/ml of basic fibroblast growth factor (bFGF), and 30 to 50 mM of It may consist of ascorbic acid and 90 to 110 mM of N-acetyl-L-glutamine (NAG), most preferably 2 ng/ml of epidermal growth factor, It may consist of 100 ng/ml basic fibroblast growth factor, 40 mM ascorbic acid, and 100 mM N-acetyl-L-glutamine, but is not limited thereto.

In addition, the CM50 is characterized by increasing the proliferation and collagen synthesis of dermal papilla cells.

In the cosmetic composition for improving hair loss or promoting hair growth of the present invention, the CM50 may be included in an amount of 1 to 10% by weight based on the total weight of the composition, but is not limited thereto.

The cosmetic composition for improving hair loss or promoting hair growth of the present invention can be used to prevent, treat, or improve alopecia, and the hair loss includes alopecia areata, hereditary androgenetic alopecia, telogen effluvium, Traumatic alopecia, Trichotillomania, Pressure alopecia, anagen alopecia, pityriasis alopecia, syphilitic alopecia (Alopecia syphilitica), seborrheic alopecia (Alopecia seborrheica), symptomatic alopecia, scarring alopecia, or congenital alopecia can be chosen

In the cosmetic composition according to one embodiment of the present invention, the cosmetic composition for improving hair loss or promoting hair growth is cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel , Skin Lotion, Skin Softener, Skin Toner, Astringent, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing It may have any one formulation selected from the group consisting of cream, body lotion and body cleanser, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, and sarcosinate , fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may further contain excipients including fungicides, hydrotropes, humectants, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.

The present invention also provides an external skin preparation for improving hair loss or promoting hair growth, containing CM50 as an active ingredient.

The external skin preparation may be prepared in the form of a cream, gel, patch, ointment, warning agent, lotion, liquid, emulsion, powder or spray, but is not limited thereto.

The present invention also provides a method for preventing or treating hair loss comprising applying the cosmetic composition to the hair loss area.

In the method for preventing or treating hair loss according to the present invention, the subject to which the cosmetic composition is applied is not particularly limited as long as it is an individual for the purpose of preventing or treating hair loss. For example, it can be applied to any object, such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cows, sheep, pigs, goats, and non-human animals and humans, and the amount or number of times the cosmetic composition is applied depends on the type of object And it can be determined according to severity, age, sex, sensitivity, treatment period, etc., but is not limited thereto.

The present invention also provides a medium composition for culturing dermal papilla cells with increased proliferative ability and collagen synthesis ability, containing CM50 as an active ingredient.

In the medium composition according to the present invention, the CM50 is as described above.

In the medium composition according to one embodiment of the present invention, the CM50 may be added to various commercially known media for culturing dermal papilla cells, but is not limited thereto.

In addition, when the medium composition containing the CM50 as an active ingredient is used for culturing dermal papilla cells, the proliferative ability and collagen synthesis ability of dermal papilla cells can be increased, and the collagen of dermal papilla cells cultured in 3 dimensions is higher than that of dermal papilla cells cultured in 2 dimensions. It is characterized by further increasing the synthesis ability.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited thereto.

Example 1. Evaluation of optimal concentration of cell growth promoting substances

In order to evaluate the optimal concentration of a cell growth promoting substance that increases dermal papilla cell proliferation rate, an assay using CCK (cholecystokinin) was performed. 1.5×10 ⁴ cells/well of dermal papilla cells were placed in a 24-well plate and cultured in two dimensions in DMEM/F12 (Dulbecco's Modified Eagle Medium Nutrient Mixture/F-12) medium, 37°C, 5% CO ₂ conditions for 24 hours. After that, the medium was removed and replaced with DMEM/F12 medium containing 2% fetal bovine serum (FBS), and starvation was performed again for 24 hours. Then, 2-100 ng/ml epidermal growth factor (EGF), 2-100 ng/ml basic fibroblast growth factor (bFGF), 4-100 mM ascorbic acid ( ascorbic acid) or 4 to 100 mM of N-acetyl-L-glutamine (NAG), respectively, and cultured for 48 hours. Thereafter, the medium was removed, and 200 μl of 5 mg/ml CCK was applied per well to react for 2 hours. was added to dissolve the formazan crystals. Then, 100 μl of each was collected from each well, transferred to a 96-well plate, and absorbance was measured at a wavelength of 540 nm using a microplate reader. The proliferation rate (%) of dermal papilla cells in each treatment group was shown with the number of cells in the control group not treated as 100%.

As a result, it was confirmed that the growth rate of dermal papilla cells in the epidermal growth factor-treated group at 2 to 100 ng/ml significantly increased compared to the untreated control group. In addition, it was confirmed that the dermal papilla cell proliferation rate of the 20-100 ng/ml basic fibroblast growth factor treatment group increased significantly compared to the control group that was not treated with anything, and the basic fibroblast growth factor treatment of less than 20 ng/ml It was confirmed that the proliferation rate of dermal papilla cells did not increase. In addition, it was confirmed that the dermal papilla cell proliferation rate of the 4-40 mM ascorbic acid-treated group significantly increased compared to the untreated control group, and it was confirmed that the dermal papilla cell proliferation rate decreased by the 40 mM ascorbic acid treatment. (Fig. 1).

Example 2. Analysis of dermal papilla cell proliferation rate of CM50

Based on the results of Example 1, 2 ng/ml of epidermal growth factor, 100 ng/ml of basic fibroblast growth factor, 40 mM of ascorbic acid and 100 mM of N-acetyl-L-glutamine were mixed to obtain CM50 was prepared, and after adding to the medium such that the final concentration of each cell growth promoting substance was as described above, the proliferation rate of dermal papilla cells was measured in the same manner as described in Example 1 above.

As a result, it was confirmed that the dermal papilla cell proliferation rate of the CM50-treated group significantly increased compared to the control group that was not treated with anything (FIG. 2).

Example 3. Analysis of collagen synthesis ability of dermal papilla cells of CM50

Collagen content was measured after treatment with CM50 while culturing dermal papilla cells in two or three dimensions. Specifically, 1.5×10 ⁴ cells/well of dermal papilla cells were placed in a 24-well plate and DMEM/ It was cultured in two dimensions using F12 medium at 37°C and 5% CO ₂ conditions. In addition, dermal papilla cells were placed in DMEM/F12 medium containing CM50, 100 units/ml of penicillin, 100 units/ml of streptomycin, and 0.25 μg/ml of amphotericin B in a 24-well plate, and the diameter was 150 according to the number of cells. ~350 μm gelatin microcarriers were added at 0.1-1.0 g per 100 ml of culture medium, and then at 37°C, 5% CO ₂ , It was cultured in three dimensions at 20-50 rpm conditions.

After culturing the medium of 2-dimensionally cultured dermal papilla cells or 3-dimensionally cultured dermal papilla cells for 72 hours, the medium is recovered and the collagen synthesized and secreted by dermal papilla cells is measured using a collagen kit (Sircol ^TM collagen assay kit). content was measured.

As a result, it was confirmed that the collagen content of dermal papilla cells in the CM50-treated group significantly increased compared to the control group that was not treated with anything. It was confirmed that the content increased by about 188% (FIG. 3).

Based on the above results, when CM50 according to the present invention is treated with dermal papilla cells cultured in three dimensions using biocompatible microcarriers, the ability to synthesize collagen is remarkably increased, making hair stronger and helping to prevent hair loss. it was found that

Example 4. Clinical trial using cosmetics containing CM50

Essence-type cosmetics (Table 1) containing CM50 of the present invention were prepared and applied to hair loss patients 1-2 times a day for 6 months to hair loss patients aged 39 to 60 years, and pictures were taken on fixed dates every month. .

Composition of cosmetics containing CM50

designation	Usage	content(%)
CM50	active ingredient	1 to 4
pentylene glycol	skin conditioning agent	0.06~0.07
sodium phosphate		0.004~0.005
lecithin		0.004~0.005
phenoxyethanol		0.006~0.007
glycerin		0.01~0.02
disodium EDTA		0.05~0.06
glycine soja (soybean) oil		0.01~0.02
Hydrogenated Phosphatidylcholine (hydrogenated phosphatidylcholine)		0.005~0.006
sodium oleate		0.007~0.008
purified water	-	up to 100

As a result, it was confirmed that the cosmetics containing CM50 had an effect of improving hair loss and promoting hair growth in hair loss patients (FIG. 4).

Claims (6)

1. A cosmetic composition for improving hair loss or promoting hair growth, containing CM50 as an active ingredient.
2. The method of claim 1, wherein the CM50 is 1 ~ 110 ng / ㎖ epidermal growth factor (epidermal growth factor, EGF), 18 ~ 110 ng / ㎖ basic fibroblast growth factor (basic fibroblast growth factor, bFGF), 3 A cosmetic composition for improving hair loss or promoting hair growth, characterized in that it consists of ~50 mM ascorbic acid and 90-110 mM N-acetyl-L-glutamine (NAG).
3. The cosmetic composition for improving hair loss or promoting hair growth according to claim 1, wherein the CM50 increases the proliferation ability and collagen synthesis ability of dermal papilla cells.
4. An external skin preparation for improving hair loss or promoting hair growth, containing CM50 as an active ingredient.
5. The external skin preparation for improving hair loss or promoting hair growth according to claim 4, wherein the external skin preparation is prepared in the form of a cream, gel, patch, ointment, warning agent, lotion, liquid, emulsion, powder or spray. .
6. A medium composition for culturing dermal papilla cells with increased proliferation and collagen synthesis, containing CM50 as an active ingredient.

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