KR20120093714A - The cosmetic composition containing n-acetyl-l-glutamine - Google Patents

Abstract

PURPOSE: A cosmetic composition including N-acetyl-L-glutamine is provided to prevent skin aging and improve wrinkles by eliminating oxidizing substances, biosynthesizing collagen, promoting cell proliferation and keratin hydration. CONSTITUTION: A cosmetic composition including N-acetyl-L-glutamine contains N-acetyl -L- glutamine as active ingredients. 0.1-15 weight% of the N-acetyl-L-glutamine is included based on the total cosmetic composition. The cosmetic composition additionally includes an emulsifier. The emulsifier is monooleic acid ester or glycerol monostearin acid ester. The cosmetic composition having oxidizing substances elimination effects includes the N-acetyl -L- glutamine as active ingredient. The cosmetic composition having collagen biosynthesis effects includes the N-acetyl -L- glutamine as active ingredient. A cosmetic composition having cell proliferation promotion effects includes the N-acetyl -L- glutamine as active ingredient. The cosmetic composition having the keratin hydration acceleration effect includes the N-acetyl -L- glutamine as active ingredient.

Description

Cosmetic composition containing N-acetyl-L-glutamine as an active ingredient {THE COSMETIC COMPOSITION CONTAINING N-ACETYL-L-GLUTAMINE}

The present invention relates to a cosmetic composition comprising N-acetyl-L-glutamine as an active ingredient, specifically N-acetyl-L- glutamine as an active ingredient by oxidizing scavenging, collagen biosynthesis, promoting cell proliferation, keratin It relates to a cosmetic composition that has the effect of inhibiting aging of the skin and improving wrinkles through hydration promotion.

Skin is generally responsible for protecting and protecting physical and chemical stimulating factors such as ultraviolet rays, chemicals, microorganisms, air pollutants and dry environment from the outermost part of the human body. Therefore, protecting the skin from ultraviolet rays is very important in preventing aging.

In order to prevent photoaging due to ultraviolet rays, there are methods of applying a moisturizer to the skin to keep the skin moist and a method of applying an antioxidant to the skin to prevent the function of oxides occurring on the skin.

In particular, in relation to the latter case, when exposed to ultraviolet light, various types of oxides such as hydroxy radicals are generated in the skin, which causes various stresses on the skin cells and causes the skin to contract very rapidly. .

The contracted cell induces metabolism in the cell to catabolism, thereby allowing the cell to survive, but in this process, polymers such as proteins inside and outside the cell are degraded. As a result, matrix proteins such as collagen or elastin are decomposed, and the reduction of these proteins is a direct cause of the decrease in the number of cells, thereby promoting aging, resulting in an increase in the depth and number of wrinkles.

Therefore, in the prevention of aging of the skin, in particular by aging by ultraviolet rays, it is very important to eliminate oxides such as hydroxy radicals, and most cosmetic compositions are intended to eliminate oxides including antioxidants.

However, simply adding an antioxidant to the cosmetic composition to prevent photoaging caused by ultraviolet light is practically hard to expect anti-aging effects. When applying a cosmetic composition containing antioxidants to the skin, the antioxidants do not pass through the tough and dense stratum corneum directly to impart antioxidant power to the cells, but only to have a local role of eliminating oxides produced outside the cells. This is because it is difficult to relieve the effect of a given oxide in the cell.

Therefore, it is necessary to develop a cosmetic composition that efficiently removes a given oxide in a cell and promotes hydration of keratin, and to develop a cosmetic composition using a substance exhibiting various effects such as promoting collagen biosynthesis and cell proliferation. .

The present invention has been made to solve the above problems, and in particular, by including N-acetyl-L- glutamine as an active ingredient to overcome the stress given from the oxide generated by ultraviolet light, furthermore It is an object of the present invention to provide a cosmetic composition having an effect of inhibiting aging of the skin and improving wrinkles by promoting collagen biosynthesis, promoting cell proliferation, and promoting keratin hydration.

Cosmetic composition of the present invention for achieving the above object is characterized in that it comprises N-acetyl-L- glutamine as an active ingredient.

Here, the N-acetyl-L- glutamine may be 0.1 to 15% by weight of the total cosmetic composition.

Here, the cosmetic composition may further include an emulsifier.

Here, the emulsifier may be selected from monooleic acid ester or glycerol monostearic acid ester.

On the other hand, the cosmetic composition of the present invention for achieving the above object includes having an oxidizing scavenging effect by including N-acetyl-L- glutamine as an active ingredient.

In addition, the cosmetic composition of the present invention includes one having N-acetyl-L-glutamine as an active ingredient and having collagen biosynthetic efficacy.

In addition, the cosmetic composition of the present invention includes one having N-acetyl-L-glutamine as an active ingredient and having cell growth promoting effect.

In addition, the cosmetic composition of the present invention includes those having a keratin hydration promoting effect by including N-acetyl-L-glutamine as an active ingredient.

Hereinafter, the present invention will be described in more detail.

Cosmetic composition of the present invention comprises N-acetyl-L- glutamine as an active ingredient, the N-acetyl-L- glutamine is a material prepared for food, additives added to various drinks and liquid foods, such as sports drinks It is known as a safe substance.

Generally, when glutamine is added to a liquid product, it cannot easily react with other substances to show its original function, resulting in low stability. However, by adding an acetyl group, glutamine can improve its stability and thus exhibit a function in a liquid product. It can also be usefully used for phosphorus cosmetic composition. Since N-acetyl-L-glutamine, the active ingredient of the present invention, is transformed into glutamine after being absorbed by the human body, it can be commercialized in a stable form without deterioration of function.

<Oxidizing Scavenging>

The N-acetyl-L-glutamine is introduced into the cell through the K + / Na + transport channel in the same manner as the glutamine, and in the process, water is introduced into the cell together with the cell swelling phenomenon. When water is excessively introduced into the cell, the cell recognizes that the concentration of the components contained in the cell is relatively low, and sends a signal (a activating signal) in the direction of synthesizing the substance.

That is, keratinocytes and fibroblasts present in the outer layer of the human body are contracted by hydrogen peroxide, and the cells release water to the outside to catalyze the condition within the cells and cause epidermal damage. In this case, the cells strongly absorb N-acetyl-L-glutamine to protect the cells from such adverse effects or to induce moisture by using K + / Na + transport channels, and may contain an excess of N-acetyl- Due to the properties of L-glutamine, water enters the cell. Thus, N-acetyl-L-glutamine plays a role of scavenging oxides and normalizing cells by the method of excessive influx of cells.

Collagen Biosynthesis

In addition, the N-acetyl-L- glutamine is introduced into the cell in the same manner as described above, the incoming water is recognized as a low concentration of organic matter in the cytoplasm to change the intracellular metabolic signal from catabolism to assimilation Can be induced to. For this reason, the cell synthesizes a polymer material inside and outside the cell, and in particular, human dermal fibroblast (Human dermal fibroblast-neonatal, HDFN) to induce and promote collagen synthesis can help to improve wrinkles and enhance elasticity.

<Promoting Cell Proliferation>

Glutamine is used directly in the biosynthesis of nucleotides such as purine and pyrimidine, along with its role as an amino acid that increases cell growth and the production of recombinant proteins. It induces ATP biosynthesis of energy sources necessary for intracellular protein replacement and nutrient transport, cell growth and migration, and cell integrity. Like glutamine, N-acetyl-L-glutamine also exhibits anti-aging effects by inducing intracellular ATP energy biosynthesis to promote cell proliferation and activity.

<Hydration promotion of keratin-moisturizing effect>

The N-acetyl-L-glutamine of the present invention has a moisturizing effect, wherein the moisturizing effect not only maintains the moisture present in the keratin, but also the moisture present in the keratin layer in a short time to transfer the moisture to the keratin. It prevents evaporation and helps skin function normally in keratin.

The N-acetyl-L-glutamine is composed of small molecules having a molecular weight of only 180, but may contain a very large amount of water in consideration of the size of the molecule, and thus has a function of transferring moisture with the transfer of a substance, which is a cell hydration mechanism. The cell swelling mechanism is similar to hydrating cells. Since the stratum corneum consists of hard proteins, it is very important to supply moisture to the stratum corneum through low molecular weight materials.

N-acetyl-L- glutamine of the present invention having the above-mentioned action is preferably 0.1 to 15% by weight of the total weight of the cosmetic composition. When N-acetyl-L-glutamine is less than 0.1% by weight it is difficult to show the intended effect, when it exceeds 15% by weight because the anti-aging effect is not high relative to the content and is not easy to formulate into a cosmetic composition.

In addition, the cosmetic composition of the present invention may include an emulsifier, but there is no limitation on the type of emulsifier used in the cosmetic composition of the present invention, particularly when monooleic acid ester or glycerol monostearic acid ester is effectively used. You can get it.

When the monooleic acid ester is used as the emulsifier, it is effective to contain 0.5 to 3% by weight of the total weight of the cosmetic composition of the present invention, and 0.5 to 5% by weight of the total weight of the cosmetic composition when the glycerol monostearic acid ester is used. to be.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions, in addition to the above-mentioned N-acetyl-L-glutamine and emulsifiers, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. Conventional adjuvants such as, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto.

More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

As the cosmetically effective carrier contained in the cosmetic composition of the present invention, a carrier commonly used in the art may be used depending on the dosage form.

When the formulation of the present invention is an ointment, a paste, a cream or a gel, carrier oils include animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide. Can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 And 3-butylglycol oils, in particular cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like can be used as carrier components. have.

Moreover, the cosmetic composition of the present invention may include other auxiliaries in addition to the carrier, and may include, for example, preservatives, antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings.

According to the cosmetic composition comprising N-acetyl-L-glutamine of the present invention as an active ingredient, anti-stress, cell proliferation, collagen biosynthesis by the cell swelling mechanism of N-acetyl-L- glutamine, It shows the effect of keratin hydration promotion, and is excellent in preventing photoaging, moisturizing and wrinkle improvement.

1 is a graph showing the proliferation rate of cells when treated with 1.0% to 0.004% of N-acetyl-L-glutamine in primary cultured human fibroblasts.
Figure 2 is a graph showing the survival rate of cells when treated with 0.4mM H ₂ O ₂ , 0.4mM H ₂ O ₂ + 0.25%, 0.125%, 0.031% N-acetyl-L- glutamine in primary cultured human fibroblasts to be.
FIG. 3 shows 5 × 10 ⁻² mg / mL of L-ascorbic acid, 5 × 10 ⁻³ mg / mL, and 10 ⁻¹ mg / mL of N-acetyl-L-glutamine in primary cultured human fibroblasts. ^When the concentrations of ^-2 mg / mL and 10 ^-3 mg / mL were treated, the expression level of procollagen TYPE-1 was verified by Western blotting.
Figure 4 is to test the keratin hydration efficacy of N-acetyl-L- glutamine keratin by applying distilled water as a control, 0.5% hyaluronic acid as a control group, and 0.5% N-acetyl-L- glutamine as a test group to the skin This is the result of measuring the hydration rate of.

Hereinafter, preferred embodiments of the present invention will be described in detail so that those skilled in the art may easily implement the present invention.

Prior to this, the terms or words used in this specification and claims should not be limited to the usual or dictionary meanings, and the inventors will be required to properly define the concepts of terms in order to best describe their invention. It should be interpreted as meaning and concept corresponding to the technical idea of the present invention based on the principle that it can.

Therefore, the configuration of the embodiments described herein is only one of the most preferred embodiments of the present invention and does not represent all of the technical idea of the present invention, various equivalents and modifications that can replace them at the time of the present application It should be understood that there may be

Manufacturing example  N-acetyl-L- Glutamine  Preparation of the containing composition

To the cosmetic composition consisting of the composition components as shown in Table 1 and the control as a control was added to distilled water without adding N-acetyl-L- glutamine, 0.1 to 15.0% by weight of N-acetyl-L- glutamine And various cosmetic compositions were prepared by adding cosmetic and pharmaceutically acceptable adducts.

Composition (% by weight) of N-acetyl-L-glutamine containing composition ingredient Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 end ethanol
N-acetyl-L-glutamine
glycerin
1,3-butylene glycol
Arginine
Sterile purified water 5.0
-
4.0
4.0
-
68.5 5.0
0.1
4.0
4.0
0.1
68.3 5.0
1.0
4.0
4.0
0.9
66.6 5.0
5.0
4.0
4.0
4.5
59.0 5.0
10.0
4.0
4.0
9.0
49.5 5.0
15.0
4.0
4.0
13.5
40.0 I ethanol
Cetyl alcohol
Wax
Waserin
Squalene
Dimethyl Polysiloxane
POE (10) Monooleic Acid Ester
Glycerol Monostearic Acid Ester
antiseptic
Spices
Pigment 5.0
1.0
0.5
2.0
6.0
2.0
1.0
1.0
Suitable amount
Suitable amount
Suitable amount 5.0
1.0
0.5
2.0
6.0
2.0
1.0
1.0
Suitable amount
Suitable amount
Suitable amount 5.0
1.0
0.5
2.0
6.0
2.0
1.0
1.0
Suitable amount
Suitable amount
Suitable amount 5.0
1.0
0.5
2.0
6.0
2.0
1.0
1.0
Suitable amount
Suitable amount
Suitable amount 5.0
1.0
0.5
2.0
6.0
2.0
1.0
1.0
Suitable amount
Suitable amount
Suitable amount 5.0
1.0
0.5
2.0
6.0
2.0
1.0
1.0
Suitable amount
Suitable amount
Suitable amount

Example  1: N-acetyl-L-glutamine is a human fibroblast ( Human Dermal  Fibroblast Neonatal Effect on the growth of

In this example, the effect of N-acetyl-L-glutamine on the proliferation and activity of the cells was verified by comparing the cell viability after treatment with cultured human fibroblasts by concentration of N-acetyl-L- glutamine.

MTT material is absorbed into cells to form formazan by succinate dehydrogenase in mitochondria. Intracellular accumulation of this material means mitochondrial activity, and broadly cell activity, so that cell proliferation can be evaluated. Cells cultured in 96-well plates were treated with N-acetyl-L-glutamine at a concentration of 1.0% to 0.004% and incubated in a 5% CO 2, 37 ° C. incubator for 48 hours. After reacting the MTT solution for 3-4 hours, the OD value was measured at 540 nm with an ELISA meter. The cell viability calculation formula is as follows.

Cell viability (%) = (OD at 540 nm in sample addition group) / OD at 540 nm in sample no addition group × 100

The test results of the DMSO treatment group as a control and the addition of N-acetyl-L-glutamine are shown in FIG. 1. As a result of the test, the N-acetyl-L-glutamine treated group in Example 1 showed a cell survival rate of 110% or more. Therefore, it was confirmed that N-acetyl-L-glutamine has an effect of further promoting the proliferation and activity of human fibroblasts.

Example  2: Validation of N-acetyl-L-Glutamine on the Protection of Human Fibroblasts Exposed to Oxidative Stress

In this example, the cultured human fibroblasts were exposed to hydrogen peroxide to induce oxidative stress, and then the cell survival rate was compared to verify the efficacy of N-acetyl-L-glutamine protecting the cells from oxidative stress.

Human fibroblasts were treated with hydrogen peroxide, hydrogen peroxide + N-acetyl-L-glutamine, and cell viability was measured by MTT analysis after 24 hours. Cells were measured for the OD value at 540nm using a microplate. The cell viability calculation formula is as follows.

Cell viability (%) = (OD at 540 nm in sample addition group) / OD at 540 nm in sample no addition group × 100

The test results of the hydrogen peroxide treated group as a negative control and the addition of N-acetyl-L-glutamine and hydrogen peroxide + N-acetyl-L-glutamine are shown in FIG. 2. As a result, the cell survival rate of hydrogen peroxide, a negative control group in Example 2, was 57%, whereas the N-acetyl-L-glutamine 0.125% and 0.031% treatment groups showed a cell survival rate of 100% or more. Therefore, it was confirmed that N-acetyl-L-glutamine has an effect of protecting human fibroblasts from oxidative stress induced by hydrogen peroxide and further improving growth.

Example  3: N-acetyl-L-Glutamine Procollagen in Human Fibroblasts TYPE Of efficacy on -1 biosynthesis

Western blotting was used to verify the procollagen type 1 biosynthesis effect of N-acetyl-L-glutamine.

After primary cultured human fibroblasts were placed in a culture flask and attached to the bottom, N-acetyl-L-glutamine diluted by concentration and L-ascorbic acid as a control thereof were added to the culture medium, whereby the cells contained 70 to 70 microns in the floor area. It was incubated for 24 to 48 hours until the growth of 80% and the supernatant was recovered. The collected supernatant was placed in 5 x SDS sample buffer and heated at 100 ° C for 5 minutes to use as a sample. After electrophoresis at 80 V on a 10% SDS polyacrylamide gel, it was transferred to a nitrocellulose membrane and reacted for 1 hour in a blocking solution containing 5% skim milk. The primary antibody COL1A1 (sc-25974) was diluted at a ratio of 1: 1000 and reacted at room temperature for 2 hours, and then washed four times with TBS-T buffer for 5 minutes. The reaction was performed at room temperature for 2 hours with a secondary antibody diluted at a ratio of 1: 2000, and then washed 4 times with TBS-T buffer. Finally, the reaction was carried out using a Western blot detection kit and developed into an X-ray film.

The test results performed by adding L-ascorbic acid as a positive control and adding N-acetyl-L-glutamine are shown in FIG. 3. As a result of the test, N-acetyl-L-glutamine in Example 3 showed an expression amount of procollagen TYPE-1 similar to L-ascorbic acid as a positive control. Therefore, it was confirmed that N-acetyl-L-glutamine has an effect of further improving procollagen TYPE-1 biosynthesis in human fibroblasts depending on the concentration.

Example  4: moisturizing effect test

Example  4-1. N-acetyl-L-glutamine in humans Keratinous  Moisturizing effect due to fast hydration

For testing, N-acetyl-L-glutamine 0.5% was prepared, and a hyaluronic acid 0.5% sample was prepared as a comparative group, and distilled water was used as a control. The moisture content of the skin was measured before applying the sample to determine the basic moisture content of the skin. 0.1 mL of each sample was applied to the skin, and after 10 seconds, 30 seconds, and 60 seconds, the application area was wiped off with a soft towel, and skin moisture content was measured. The moisture content was measured at least 5 times at a predetermined position and then the average value was calculated. The skin volume meter CM825 was used to measure skin moisture.

The moisturizing effect of keratinization of N-acetyl-L-glutamine is shown in FIG. 4. As a result, N-acetyl-L-glutamine hydrated keratin faster than hyaluronic acid, a control group including distilled water, to increase the moisture content of the skin, and the ability to hydrate keratin according to application time was about 30 seconds. It was found that they can hydrate keratin quickly enough to get the maximum.

Example  4-2. N-acetyl-L- Glutamine  Contained Cosmetics  Moisturizing efficacy test of the composition

In order to investigate the anti-wrinkle effect of N-acetyl-L-glutamine of the present invention, 30 women in their 30s, the cosmetic composition 1 (control group) and compositions 2 to 6 (experimental group) prepared in the above preparation on the face area, respectively It was applied twice a day for 1 month. In order to measure the improvement of skin moisturizing ability after 1 month, the skin moisture content was measured after washing and evaluated in four stages: no improvement, slight improvement, severity improvement, and significant improvement compared to before the test.

In the evaluation of the skin's moisturizing ability, the skin moisture content meter CM825 was used to measure the basic moisture content of the face before the test was started to measure the improvement of the skin moisture content. The unit of skin moisture capacity meter is expressed as capacitance value.

The results are shown in the following Table 2, it was confirmed that the composition 2 ~ 6 users containing N-acetyl-L- glutamine showed an improvement in skin moisture compared to the composition 1 users. In addition, when the composition of the present invention is applied for a long time, the moisture content of the skin itself was confirmed to increase by about 10.4 ~ 33.8%.

These test results indicate that the composition of the present invention can improve skin moisturizing power when used as an external preparation for skin, and show that the N-acetyl-L-glutamine of the present invention can be usefully used as a cosmetic raw material.

No improvement (persons) Slightly improved Severity Improvement Significant improvement Skin moisture content (capacitance value) % Change Composition 1 20 8 2 0 40.5 ± 5.3 0 Composition 2 10 9 9 2 44.7 ± 5.0 10.4 Composition 3 7 10 6 3 46.3 ± 3.0 14.3 Composition 4 0 6 14 10 51.0 ± 4.7 25.9 Composition 5 0 3 12 15 53.5 ± 1.5 32.1 Composition 6 0 5 14 11 54.4 ± 1.2 33.8

Claims (8)

Cosmetic composition containing N-acetyl-L- glutamine as an active ingredient. The method of claim 1,
The N-acetyl-L- glutamine is a cosmetic composition, characterized in that 0.1 to 15% by weight of the total cosmetic composition. The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that it further comprises an emulsifier. The method of claim 3, wherein
The emulsifier is a cosmetic composition, characterized in that one selected from monooleic acid ester or glycerol monostearic acid ester. Cosmetic composition which has an oxidative scavenging effect by including the composition of any one of Claims 1-4. Cosmetic composition which has collagen biosynthesis effect by including the composition of any one of Claims 1-4. Cosmetic composition which has a cell proliferation promoting effect by including the composition of any one of Claims 1-4. Cosmetic composition which has a keratin hydration promoting effect by including the composition of any one of Claims 1-4.

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