KR20110006212A - Hair growth promoter using low temperature nano extraction method having copper-peptide and manufacturing method thereof - Google Patents

Abstract

PURPOSE: An agent for promoting hair growth containing copper-peptide and a method for manufacturing the same are provided to effectively prevent hair loss and promote hair growth without side effects. CONSTITUTION: An agent for promoting hair growth containing copper-peptide contains 0.03-5 weight parts of nicotine amide, 0.05-15 weight parts of copper-peptide, 0.01-5 weight parts of salicylic acid, 0.05-10 weight parts of Poria cocos wolf extract, 0.03-8 weight parts of Morus alba extract, 0.5-30 weight parts of seaweed extract, and 0.5-30 weight parts of kelp extract extracted using mixture solvent of organic solvent and water at 15-60°C. The organic solvent is ethanol, methanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylenes glycol or glycerin.

Description

Hair Growth Promoter Using Low Temperature Nano Extraction Method Having Copper-peptide And Manufacturing Method Thereof}

The present invention relates to a copper-peptide-containing low-temperature nano-extraction hair growth promoter and a method for producing the same, and more specifically, to the copper-peptide-containing low-temperature nano-extraction hair growth promoter using a mixed solvent or water of an organic solvent and water 15 ~ 60 ℃ Nitrotinic acid amide, copper-peptide, salicylic acid, Bongyeong extract, lettuce extract, jinjin mugwort extract, seaweed extract and kelp extracts containing 1000-3000 나노 nanoscale copper-peptide-containing low-temperature nano hair growth promoters will be.

With the development of modern science, human beings are getting better every day and the dream of prolonging their lives is becoming a reality. Nevertheless, the solution of alopecia is still the task of humankind and is being researched and developed in many countries, research institutes and companies. The hair loss prevention and regrowth is a product that has a global marketability.

Human hair has a cosmetic function in addition to functional aspects such as protecting the head from sunlight, absorbing shock from external shock, and maintaining body temperature. In recent years, the number of female hair loss patients has increased due to changes in lifestyles such as westernized eating habits and frequent hair pollution such as perm and dyeing, and adolescent hair loss due to the fact that smoking, academic stress, and puberty itself are accelerated by an average of three years. Patients are also increasing.

Alopecia is a disease that is lost in the area where hair is normally present. It occurs mainly in the head and is classified into non-alopecia areata and alopecia areata according to the damage of hair follicles. No specific drug has been found. Minoxidil has been developed and used as an antihypertensive agent. After Dargie et al. Reported in 1977 the occurrence of hirsutism after oral administration of minoxidil, the topical coating of minoxidil began to be used as a treatment for alopecia.

This has resulted in the release of numerous unproven hair regrowth, hair growth and tonic products worldwide, and the damage caused by unproven products has long been left to consumers suffering from hair loss. Therefore, the development of new hair loss preventing agent and hair growth promoting agent is urgently needed.

In order to solve such a problem, there is known a technique related to a method for manufacturing a hair regrowth using a natural extract and a topical coating agent and a pill (Korean Patent Registration No. 476,731). However, the method of preparing the hair regrowth has a problem that the effect of hair growth is reduced because the natural extract particles acting on the hair root.

Accordingly, an object of the present invention is to solve the problems of side effects caused by conventional unproven hair loss inhibitors and hair growth promoters by producing copper-peptides, natural herbal medicines and algae-derived hair growth promoters extracted at nano-scale at low temperature. It is an object of the present invention to provide a low-temperature nano-extraction hair growth promoter containing and a method for producing the same.

In order to solve the above object, the present invention provides a hair growth promoting agent,

Copper, including nicotinic acid amide, copper-peptide, salicylic acid, bongyeong extract, lettuce extract, jinjin mugwort extract, seaweed extract, and kelp extract, extracted with nano-scale of 1000 ~ 3000Å at 15 ~ 60 ℃ using mixed solvent of organic solvent and water It provides a peptide-containing low temperature nano extraction hair growth promoter.

In addition, the present invention is a hair growth promoter,

Low-temperature nano- extraction containing copper-peptide, including nicotinic acid amide, copper-peptide, salicylic acid, Bongyeong extract, lettuce skin extract, jinjin wormwood extract, seaweed extract and kelp extract Provide a hair growth promoter.

In addition, the present invention is a hair growth promoter manufacturing method,

Nicotinic acid and copper-peptide were added to the organic solvent, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was added to the organic solvent, purified water, and seawater. Boil the leaves, black soybeans, woobangeun, black jima, sewage, cheongung and turmeric and boil them, and then extract each of the extracts at 15-60 ℃ for 24-48 hours with 20-50% mixed solvent of the organic solvent and water. After filtering with an ultrafilter, and then again with a nanofilter, separated by centrifugation, placed in a vacuum depressurizer and concentrated for 1 to 5 hours at a temperature of 30 ~ 50 ℃ evaporated organic solvent and water After the addition, to the extraction solution (B) to lower the concentration of the mixed solvent of the organic solvent and water at 0.5 to 5%, a copper-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method comprising obtaining a reddish brown uniform solution Provided.

In addition, the present invention is a hair growth promoter manufacturing method,

Nicotinic acid amide and copper-peptide were added to the purified water, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was added to the purified water and seawater, baekryepi, jinjinmyeong, wakame, kelp, pine needles, white leaf, black soybean. , Boiled, woobangeun, black jima, sewage, cheongung and jihwang boiled and extracted with water as an extraction solvent at 15 ~ 60 ℃ for 24 to 48 hours to prepare each extract, and then filtered with an ultrafilter, and again filtered with a nanofilter Then, the resultant was separated by centrifugation, placed in a vacuum depressurizer, concentrated at a temperature of 30 to 50 ° C. for 1 to 5 hours, added to the evaporated extraction solution (B), and then a reddish brown uniform solution. It provides a copper-peptide-containing low-temperature nano-extracting hair growth promoting agent comprising the obtained.

The copper-peptide-containing low-temperature nano-extracting hair growth promoter of the present invention and a method for preparing the same are used by low-temperature nano-extraction, so that the nano-sized hair-promoting agent is well absorbed by hair follicles to inhibit the expression of TGF-β2, a hair growth cell degenerative protein. Prevents hair loss, and is useful for wool, hair growth, and hair growth. In addition, copper-peptides, herbal medicines and seaweeds are used as raw materials to prevent various side effects of hormonal therapeutics and overcome shedding. In addition, it contributes to the creation of employment and the local economy, as well as the vitalization of R & D in the bio industry, which can replace the local industry, and the effect of replacing hair loss, wool, hair growth, and hair growth-related products.

The present invention is a copper-peptide-containing low-temperature nano-extracting hair growth promoter, nicotinic acid amide, copper-peptide, salicylic acid, Bongyeong extract, lettuce extract extracted at a nano size of 1000 ~ 3000Å at 15 ~ 60 ℃ using a mixed solvent of organic solvent and water Contains, Mugwort Extract, Seaweed Extract, and Kelp Extract.

The copper-peptide-containing low-temperature nano-extracting hair growth promoter further comprises pine needle extract, cypress leaf extract, black soybean extract and right root extract.

The copper-peptide-containing low-temperature nano-extracting hair growth promoting agent further comprises black jima extract, sewage extract, cheongung extract, and sulfuric acid extract.

At this time, the peptide of the copper-peptide is alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, leucine, methionine, threonine, tryptophan, The same or different amino acid residues, either of tyrosine, valine or arginine, or a mixture of two or more thereof, are amide linked peptides.

Here, the organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof.

The copper-peptide-containing low temperature nano-extracting hair growth promoter is added to the nicotinic acid amide and the copper-peptide to an organic solvent, and the main solution (A) in which benzoic acid and lavender oil, which is a flavoring agent, are sequentially added to the salicylic acid to dissolve the organic solvent. 20-50% of the above-mentioned organic solvent and water, which are extracts after boiling, purified water and sea water, Bongyeong, Sangbaekpi, Injin mugwort, seaweed, kelp, pine needles, cypress leaves, black soybeans, woobangeun, black jima, sewage, Cheongung and Jihwang Each extract was prepared by extracting at 15 to 60 ℃ for 24 to 48 hours with a solvent, and then added to the extraction solution (B) lowering the concentration of the mixed solvent of the organic solvent and water to 0.5 to 5%, and then a uniform solution Made by centrifugation, filtered by nanometer, sterilized and placed in a container. Here, the size of each functional extract is a nano size of 1000 ~ 3000Å. The hair growth promoter is well absorbed by hair follicles and inhibits the expression of TGF-β2, a hair growth cell degenerative induction protein, to prevent hair loss, and is useful for wool, hair growth, and hair growth. In other words, since the low-temperature nano extraction method is used, the nano-sized hair growth promoter is well absorbed by hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth. In addition, the copper-peptide-containing low-temperature nano-extracting hair growth promoter overcomes the shedding phenomenon. Shading is a phenomenon in which newly growing healthy hair pushes out resting hair which should be removed anyway, which is a normal process that occurs between two to six weeks after hair loss treatment, and hair falls out more than usual at this time.

At this time, the water may be purified water, distilled water or purified water.

The nicotinic acid amide is one of the water-soluble vitamin B complexes, like nicotinic acid, and exists in vivo as nicotinamide nucleotides which are coenzyme types of this vitamin, namely NAD + and NADP +, and is involved in many oxidation / reduction reactions as coenzymes of dehydrogenase. Another function of nicotinamide nucleotides is the reduction of flavincoenzyme, in which case proton addition or desorption is involved in the 4-membered pyridine ring carbon atom. The nicotinic acid amide is hydrolyzed by nicotinamidase and the resulting nicotinic acid is reused for NAD synthesis. In addition, the nicotinic acid amide is methylated in the liver to form n-methylnicotinic acid amide, which is mixed with urine and discharged.

The copper-peptide is a combination of copper and peptide and is effective for skin regeneration of hair follicles and hair regeneration. The increase in hair follicle size and hair growth rate due to the administration of the copper-peptide is caused by supplying proper nutrition to the hair follicles through the change of blood flow so that hair with thick hair grows rapidly. As an example of the copper-peptide, copper-peptide (Cu-GHK) in which copper is bonded to Glycyl-Histidyl-Lysine is a very rare sequence extracted from protein in the human body, and collagen, thrombospondin, fibrin a-chain, prokininogen, complement It exists in a strong state combined from proteins such as C1q, interleukin 4, skin collagenase, coagulation factor XI, and SPARC, which primarily protects tissues and prevents inflammation due to tissue damage. It also serves as a signal for tissue reconstruction, regenerating damaged proteins and replacing them with normal tissue. The Cu-GHK may be referred to as a feedback signal for healing and reconstructing the tissue and protecting the tissue when the tissue is damaged. In addition, the Cu-GHK has excellent skin regeneration ability to promote wound healing, strengthen skin elasticity, and increase the subcutaneous fat layer to play a role of counteracting the aging phenomenon of the skin. In addition, it is recognized as an excellent material in dermatology by increasing the success rate of skin graft surgery, and it increases the hair follicle size as a residual of GHK analogs fat to promote hair growth and reduce hair loss.

Since salicylic acid has the action of antipyretic and analgesic as o-oxybenzoic acid, it is internalized with sodium salt or calcium salt, and is used to improve solubility, stability, pharmacology, etc. by combining salicylic acid with various drugs.

The order is to protect the intestines and dried the fungal nucleus of Bokryeong, diuretic and lowering blood sugar, sedation and the like.

The baekbaekpi is a mulberry bark anti-inflammatory action, antioxidant action, skin soothing action, to prevent aging.

The jinjin mugwort is called cheonjae wormwood or Injincho and jaundice, soy sauce, cold cold, anti-inflammatory diuretics, etc. may be taken. The phosphorus mugwort contains dimethylescholetin, scopoline, essential oils, aromatic oxycarboxylic acids, flavonoids, oxycoumarins and the like in the outpost.

The seaweed is rich in dietary fiber and potassium, calcium, iodine, etc., to promote metabolism, postpartum cooking, constipation, obesity prevention, iron and calcium supplements are excellent.

In addition to various pigments such as carotene, xanthophylls, and chlorophyll, the kelp contains a lot of carbohydrates, such as manit and laminarin, which are made by carbon assimilation, and alginic acid, which are components of cell walls, and iodine, vitamin B2, glutamic acid, and the like. Contains amino acids The kelp contains laminin, which lowers blood pressure.

The pine needles are an ingredient of oxyl palmitic acid is effective for skin, beauty and heart strengthening.

The white leaf has a hemostatic action.

In the black soybean, aggregation of platelets is suppressed.

The right root is the root of burdock soothes the sore face and treats sore throat, boil, mange and ringworm. It has been reported that pharmacological action also acts against the various filamentous fungi that cause cancer, activate liver function, purulent bacteria and skin disease.

The black jima is a black sesame to promote the recovery of the skin mucous membranes, greatly reduce the cholesterol level in the blood, and activate the bowel movements.

The sewage is tonic action, hematopoietic function strengthening, fatigue recovery action.

The uterus has a sedation, blood pressure lowering action, antibacterial action.

The sulfur is lowering blood sugar, diuretic, antibacterial action.

The content of the nicotinic acid amide of the hair growth promoter is 0.03 to 5 parts by weight, the content of the copper-peptide is 0.05 to 15 parts by weight, the content of the salicylic acid is 0.01 to 5 parts by weight, and the content of the extract is 0.05 to 10 parts by weight. Part, the content of the extract of the lettuce extract is from 0.03 to 8 parts by weight, the content of the jinjinjin mugwort extract is from 0.05 to 10 parts by weight, the content of the seaweed extract is 0.5 to 30 parts by weight and the content of the kelp extract is 0.5 to 30 parts by weight. .

When the content of each of the components is less than the above range, it is difficult to exhibit hair growth, and when the content of each of the ingredients exceeds the above range, side effects may be exhibited.

In addition, the content of the pine needle extract of the hair growth promoter is 0.5 to 10 parts by weight, the content of the cypress leaf extract is 0.5 to 10 parts by weight, the content of the black soybean extract is 0.05 to 8 parts by weight, and the content of woobangeun extract 0.03 to 5 parts by weight.

When the content of each of the components is less than the above range, it is difficult to exhibit hair growth and skin soothing action, and when the content of each of the above components exceeds the range, side effects may be exhibited.

In addition, the content of the black jima extract of the hair growth promoter is 0.05 to 8 parts by weight, the content of the sewage extract is 0.03 to 5 parts by weight, the content of the cheongung extract is 0.03 to 5 parts by weight, and the content of the sulfuric acid extract is 0.03-7 weight part.

When the content of each component is less than the above range, it is difficult to exhibit hair growth and skin pharmacological action, and when the content of each of the above components exceeds the range, side effects may be exhibited.

In addition, the copper-peptide-containing low-temperature nano-extracting hair growth promoter of the present invention, nicotinic acid amide, copper-peptide, salicylic acid, bongyeong extract, lettuce extract, jinjin mugwort extract extracted with nano-sized nanoscale of 1000 ~ 3000Å at 15 ~ 60 ℃ using water Seaweed extract, and seaweed extract.

Here, the copper-peptide-containing low-temperature nano-extracting hair growth promoter is added to the nicotinic acid amide and the copper-peptide to purified water, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil as a flavoring agent to the salicylic acid. Bongyeong, Sangbaekpi, Injin mugwort, seaweed, seaweed, seaweed, pine needles, cypress leaves, black soybean, woobangeun, black jima, sewage, cheongung and jihwang, boiled, and cooled with water as an extraction solvent for 15 to 60 hours. After extracting at ℃ ℃ to prepare each extract, water was added to the evaporated extraction solution (B), then made into a uniform solution separated by centrifugation, filtered by nanometer, sterilized and put in a container. At this time, the size of each functional extract is a nano size of 1000 ~ 3000Å. The hair growth promoter is well absorbed by hair follicles and inhibits the expression of TGF-β2, a hair growth cell degenerative protein, to prevent hair loss, and is useful for wool, hair growth, and hair growth. In other words, since the low-temperature nano extraction method is used, the nano-sized hair growth promoter is well absorbed by hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth. In addition, the copper-peptide-containing low-temperature nano-extracting hair growth promoter overcomes the shedding phenomenon.

At this time, the water may be purified water, distilled water or purified water.

In addition, the copper-peptide-containing low-temperature nano-extraction hair growth promoter of the present invention,

Nicotinic acid amide and copper-peptide were added to the organic solvent, and benzoic acid and lavender oil were sequentially added to salicylic acid to dissolve the main solution (A) in the organic solvent, purified water, and seawater. Boiled the baekbaek leaf, black soybeans, woobangeun, black jima, sewage, cheongung and jihwang, and then extracted with 20 to 50% mixed solvent of the organic solvent and water as an extraction solvent at 15 ~ 60 ℃ for 24 to 48 hours to extract each extract After the preparation, the resultant was filtered with an ultrafilter, again filtered with a nanofilter, separated by centrifugation, placed in a vacuum vacuum concentrator, and then concentrated at a temperature of 30-50 ° C. for 1-5 hours to obtain an organic solvent and water. After evaporation, the mixture was added to the extraction solution (B) having a lowered concentration of the mixed solvent of organic solvent and water at 0.5 to 5% to obtain a reddish brown uniform solution.

At this time, the peptide of the copper-peptide is alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, leucine, methionine, threonine, tryptophan, The same or different amino acid residues, either of tyrosine, valine or arginine, or a mixture of two or more thereof, are amide linked peptides.

Here, the organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof.

At this time, the water may be purified water, distilled water or purified water.

In addition, the copper-peptide-containing low-temperature nano-extraction hair growth promoter of the present invention,

Nicotinic acid amide and copper-peptide were added to the purified water, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was added to the purified water and seawater, baekryepi, jinjinmyeong, wakame, kelp, pine needles, white leaf, black soybean. , Boiled, woobangeun, black jima, sewage, cheongung and jihwang boiled and extracted with water as an extraction solvent at 15 ~ 60 ℃ for 24 to 48 hours to prepare each extract, and then filtered with an ultrafilter, and again filtered with a nanofilter Then, the resultant was separated by centrifugation, placed in a vacuum depressurizer, concentrated at a temperature of 30 to 50 ° C. for 1 to 5 hours, added to the evaporated extraction solution (B), and then a reddish brown uniform solution. To get.

At this time, the water may be purified water, distilled water or purified water.

Here, the content of the nicotinic acid amide is 0.03 to 5 parts by weight, the content of the copper-peptide is 0.05 to 15 parts by weight, the content of the salicylic acid is 0.01 to 5 parts by weight, the content of the seaweed extract is 0.05 to 30 parts by weight, The content of the kelp extract is 0.5 to 30 parts by weight, the content of the pine needle extract is 0.5 to 10 parts by weight, the content of the cypress leaf extract is 0.5 to 10 parts by weight, the content of the black soybean extract is 0.05 to 8 parts by weight, The content of woobangeun extract is 0.03 ~ 5 parts by weight, the content of the black jima extract is 0.05 ~ 8 parts by weight, the content of the sewage extract is 0.03 ~ 5 parts by weight, the content of the cheongung extract is 0.03 ~ 5 parts by weight, The content of the Bokryeong extract is 0.05 to 10 parts by weight, and the content of the turmeric extract is 0.03 to 7 parts by weight.

The above description of each extract and the content of each extract uses the preceding description.

In addition, the copper-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method is a pre-extraction preparation to perform the selection, washing, drying or crushing of the raw materials.

Here, the copper-peptide-containing low-temperature nano extraction hair growth promoter manufacturing method is a raw material extraction by applying the step-by-step low-temperature nano- extraction method to the target object by using ultrasonic waves after the screening, washing process using natural herbal ingredients and seaweeds in the production of hair loss prevention products It can be manufactured in a way that maximizes the effectiveness and extraction of specific components.

In addition, the copper-peptide-containing low-temperature nano extraction hair growth promoter manufacturing method will be described with drawings.

1 is a process chart showing a method for preparing a copper-peptide-containing low-temperature nano-extracting hair growth promoting agent of the present invention.

As shown in Figure 1, after preparing a copper-peptide, algae, natural herbal medicines, herbal medicines, and other materials, the preparation before extraction, such as sorting, washing, drying, crushing.

Then, each raw material is extracted for each raw material extracted with a mixed solvent of organic solvent and water or purified water.

After extracting the raw materials, the ultrafiltration process is performed, and the extract which is difficult to be separated by normal filtration is filtered by using dialysis paper using a sulfate sheet, a bladder membrane, and colloidion.

Thereafter, a nanofiltration process is performed using a nanofilter on the extract obtained by the ultrafiltration process.

Then, the resultant is separated by centrifugation, placed in a vacuum vacuum concentrator and concentrated, and then the organic solvent or water is evaporated to lower the alcohol concentration to produce an extract (B).

In the case of extraction with purified water, the purified water is evaporated to generate an extraction solution (B).

Thereafter, nicotinic acid amide and copper-peptide are added to the organic solvent or purified water, and benzoic acid and lavender oil are sequentially added to salicylic acid and added to the dissolved main solution (A). Produces a product which is a reddish brown homogeneous solution.

In addition, the hair loss management system of the present invention consultation and shooting; Primary sampu; Scalp steamer; Scalp peeling and oxygen supply; Infrared irradiation; Ampoule application; Scalp massage; And secondary sampu; sequentially.

In the counseling and photographing step, when a baldness visits, a person consults about eating habits and hair hygiene, and photographs a bald area with a digital camera. At this time, shoot with the hair enlarger.

In the first sampu step to remove the first contaminants such as foreign substances by scalp cleaning before management.

The scalp steamer step facilitates the removal of fine contaminants remaining in the scalp (mogu) by steam and absorption of hair growth promoter ampules.

In the scalp peeling and oxygen supplying step, oxygen is supplied to the scalp and the contaminant filling and scalp such as foreign matter in the scalp (mogu) relaxed by steam.

In the infrared irradiation step helps to circulate blood using infrared rays.

In the ampoule application step, the hair growth promoter ampoule for nourishing the scalp is finely sprayed with an oxygen jet spray.

In the scalp massage step to massage the scalp to improve the blood circulation of the scalp to increase the absorption of the hair growth promoter ampoule.

Finally, in the second shampoo phase, the final shampoo after absorbing the hair growth promoter ampoule creates a personal style.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

EXAMPLES Material Extraction and Methods

After sorting each material (natural herbal medicine and algae), it is prepared by washing, drying, crushing, etc. for detailed selective extraction according to the functional material of each raw material. It was made to include the extract. The extracted active ingredients are again subjected to primary extraction and concentration using ultrafiltration (ultrafiltration, MWCO, 20,000), followed by extraction and concentration according to molecular weight in the second order through nanofiltration (nanofiltration, MWCO, 10,000). As an example of the copper-peptide-containing low-temperature nano extract hair growth promoter (product name: upgrade-JW12) was prepared (see Fig. 2).

The composition and content of the extract in 100 ml of copper-peptide-containing low-temperature nano-extracting hair growth promoter (product name: upgrade-JW12) of the present invention are as follows.

The content of nicotinic acid amide is 0.1g, copper-peptide (Cu-GHK) is 0.5g, salicylic acid is 0.1g, seaweed extract is 3g, kelp extract is 4g, pine needle extract is 2.5g , The content of the cypress leaf extract is 2.5g, the content of the black soybean extract is 1g, the content of the right root extract is 0.5g, the content of the black jima extract is 1g, the content of the sewage extract is 0.5g, the content of the cheongung extract is 0.3g, It contains 0.3g of Bongyeong extract, 0.8g of lettuce extract, 0.5g of Injin mugwort extract, and 0.5g of Ginseng extract, and adds 0.2g of benzoic acid as a preservative, and adds ravenda oil as a flavoring agent. Add 0.1 g, add 30 g of ethanol, and add rest and purified water.

Experimental Example 1

Content of experiment

end. Establish standardization of extraction, separation, purification and concentration of functional ingredients from seaweeds and natural herbs through low temperature nano extraction

(1) In order to prevent hair loss and maximize the effects of wool and hair growth, functional bioactive substances that require specific heat treatment of raw materials were extracted and obtained at 50 ° C or below.

(2) Functional bioactive materials requiring organic solvent extraction were extracted from 50% organic solvent.

(3) Natural herbal medicines were separated / filtered secondly after the first hot water extraction.

(4) The concentration of the extract was tested for efficacy at 25% or 50% concentration (water and alcohol removal) using a general vacuum evaporation at 40 ° C.

I. Analysis of General Components of Extracts

Component analysis was conducted in 1st (2006/08/30), 2nd (2006/10/21), 3rd (2006/12/21), and 4th (2007/02/22).

(1) Analysis

 1) Elemental Analysis

ICP (Inductively Coupled Plasma Emission Spectrometer, Thermo Jarrel Ash Co., IRIS / AP) was used to investigate the elemental content and content of the sample. Sample (3 g) was added H ₂ O ₂ 2 to HN ₃ 10 and digested with Microwave (Malstone Co.,) and filled up with standard amount 20 with 2-3% HNO ₃ .

 2) Free Sugar Analysis

   HPLC (High Performance Liquid Chromatography, Waters Co., Sugarpak Icolumn) was used to investigate the free sugar component and content of the samples.

   Sample 1 was filtered through a microfilter and used as the sample.

 3) amino acid analysis

   An automatic amino acid analyzer (Automatic Amio Acid Analyzer, Pharmacia Biotech Co., Biochrom 20, Na form column) was used to investigate the amino acid content and content of the sample.

   The sample was hydrolyzed with 6N hydrochloric acid (HCl), filtered through a microfilter, and used as a sample.

 4) Microbial Analysis

   In order to analyze the microorganisms in the sample, the presence of Escherichia coli, bacteria, actinomycetes, yeast, and fungi was analyzed by the general Bergy's mannul method and 3M Petrifilm.

(2) Analysis result

 1) Elemental Analysis (ppm) of the product (Upgrade-JW12; Hair Growth Promoter)

Element JW0830 JW1021 JW1221 JW0222 K 1455.5 1779.9 1705.2 1517.3 Ca 465.6 483.6 504.5 406.8 Mg 699.5 782.5 746.6 904.6 P 97.4 106.7 114.2 69.6 B 15.3 28.7 14.9 0 Mo 0.3 0.2 0.3 0.6 Si 9.9 11.6 10.3 8.5 Fe 7.9 6.8 5.1 3.2 Mn 9.6 10.3 10.8 9.3 Cu 1235.6 1358.5 1267.7 1373.7 CD 0 0 0 0 Cr 2 2.1 2.2 2.2 Pb 0 0 0 0 Zn 0.6 0.2 0.3 0 Ni 1.7 1.9 1.8 1.7 Ti 0 0 0 0 Al 0.4 0.3 0.3 0

2) Analysis of free sugar (%) of the product (upgrade-JW12; hair growth promoter)

Name JW0830 JW1021 JW1221 JW0222 Glucose 9.96 8.66 9.52 9.31 Sucrose 13.17 13.09 8.93 7.50 Fructose 10.76 11.38 13.96 15.55 Maltose 0.00 0.00 0.00 0.00 Lactose 0.00 0.00 0.00 0.00

3) Microbiological analysis (cfu / ml) of the product (Upgrade-JW12; hair growth promoter)

Microorganism name JW0830 JW1021 JW1221 JW0222 Escherichia coli 0.00 0.00 0.00 0.00 General bacterial count 0.00 0.00 0.00 0.00 Actinomycetes 0.00 0.00 0.00 0.00 Yeast 0.00 0.00 0.00 0.00 Mold water 0.00 0.00 0.00 0.00

4) Amino acid analysis (ppm) of the product (Upgrade-JW12; hair growth promoter)

No. Name JW0830 JW1021 JW1221 JW0222 One Asp 155.09 100.59 146.49 130.27 2 Thr 32.23 23.35 22.98 20.32 3 Ser 28.44 27.24 22.09 20.61 4 Glu 274.48 197.77 242.21 240.09 5 Pro 42.00 32.56 31.53 31.42 6 Gly 735.25 823.33 636.23 728.50 7 Ala 55.13 36.89 0.00 5.43 8 Cys 41.46 19.68 22.90 19.35 9 Val 0.00 26.54 4.56 14.25 10 Met 0.00 0.00 0.00 0.00 11 Ile 17.86 16.56 23.43 19.36 12 Leu 26.66 15.86 24.16 25.23 13 Tyr 0.00 0.00 16.69 14.32 14 Phe 18.15 10.67 0.00 0.00 15 His 500.68 657.42 589.00 673.00 16 Lys 840.99 827.78 733.67 631.46 17 Amm 168.54 146.00 156.96 160.69 18 Arg 0.00 0.00 0.00 0.00

5) Catechin analysis of product (upgrade-JW12; hair growth promoter) (unit: mg%)

(-) EGC (+) C (-) EC (-) EGCG (-) ECG 67.359 3.057 3.984 9.125 0.969

6) Vitamin component of the product (Upgrade-JW12; hair growth promoter) (Unit: mg / 100g)

Vitamin b1 Vitamin b2 Vitamin b6 Vitamin c 0.108 16.626 3.639 5.987

All. Long term preservation test of extract

Upgrade-JW12 is divided into 1st (2007/02), 2nd (2007/04), 3rd (2007/06), and 4th (2007/08) experiments to change the organic and phenolic acid components at two-month intervals. (Table 7, FIG. 3; Table 8, FIG. 4).

 (1) Change of organic acid according to storage period

Storage time
(Month) Organic acid (Unit: mg%) Oxalic Citric Malic Malonic Succinic Fumaric Upgrade-JW12-02February 25.26 387.27 254.56 59.79 884.90 0.50 Upgrade-JW12-04 25.39 304.59 249.89 58.89 1430.87 0.66 Upgrade-JW12-June 25.18 305.89 252.08 58.26 1429.88 0.64 Upgrade-JW12-08 25.12 317.94 245.69 79.85 1469.56 0.60

(2) Phenolic Acid Changes According to Storage Period

Storage time
(Month) Phenolics (Unit: mg%) Caffeic Chlorogenic p-Coumaric Salicylic Ferulic Cinamic Upgrade-JW12-02February 5.42 1.04 0.89 0.85 1.70 0.12 Upgrade-JW12-04 9.58 1.32 1.02 1.02 1.68 0.11 Upgrade-JW12-June 9.87 1.43 1.05 1.04 1.61 0.11 Upgrade-JW12-08 9.89 1.43 1.03 1.04 1.61 0.10

la. Consignment institution (Department of Oriental Medical Materials Engineering, Kyung Hee University)

(1) Measurement of proliferative effect of hair-forming cells on upgrade-JW12

  1) Experimental material

    Human hair follicle cells, human dermal papilla cells (HHDPC) were used as hair-forming cells, and cell culture medium was cultured using MSC and subcultured once every 3 days.

2) Sample Preparation

As a test substance, the concentration of the natural product extract, Upgrade-JW12, was used at a final concentration of 0.05%, 0.1%, and 0.5%, and H ₂ O was used as a negative control.

3) Cell growth rate measurement

    Proliferation evaluation as follows by quantifying the formation of formazan pigment by living mitochondria by 3- [4,5-dimethylthiazol-2-yl] -2 and 5-diphenyltetraolium bromide test (MTT) method Was carried out.

① The HHDPC cells were adjusted to an initial cell concentration of about 1 × 10 ⁴ cells / well per well using a 96-hole plate incubator.

② The treatment of the sample (Upgrade-JW12) was dissolved in H ₂ O as a solvent, the serum content was adjusted to 5% concentration, and then diluted sequentially with MSC medium.

③ The medium was adjusted to an amount of about 200ml per well and incubated in 37 ° C. and 5% CO ₂ incubator for 3 days.

   ④ 50 ml of the MTT solution prepared at a concentration of 2 mg / ml was added per well, and left for 4 hours in the same incubator.

   ⑤ When the MTT reagent was determined to be absorbed into the intracellular mitochondria, the supernatant was removed, 150 ml of DMSO was added, and stirred for 10 minutes to uniformly dissolve formazan dye.

   ⑥ After that, the absorbance value was measured at 540 nm using an ELISA reader, and the cell number was measured according to a standard sample curve prepared in advance.

 (2) Modulation of TGF-β2 Expression, a Parental Growth Cycle Regulatory Protein by Upgrade-JW12

  1) Experimental material

   ① Human keratinocyte HaCaT was used as hair-forming cells, and cell culture medium was cultured using RPMI and subcultured once every 3 days.

   ② Human hair follicle cells, human dermal papilla cells (HHDPC) were used, and cell culture medium was cultured using MSC, and passaged once every 3 days.

2) Preparation of Sample

    As a test substance, a natural product extract, Upgrade-JW12, was used, and a final concentration of 0.1% or 1% was used.

3) Measurement of TGF-β2 Expression

① Incubate HaCaT and HHDPC in 60mm dish and incubate for 24 hours in 0.5% FBS condition medium when each cell shows 70% confluency. Upgrade-JW12 sample with 0.5% FBS condition solution to make final concentration 0.1% and 1%. Incubate for 24 hours, and set untreated group (-) as a control, and harvest each cell with TRIzol reagent after 24 hours incubation.

  ② Isolate total RNA from each cell by chromoform extraction and isopropanol precipitation method and quantify RNA.

  ③ Synthesize TGF-β2 cDNA using reverse transcriptase from total RNA isolated using TGF-β2 specific primer using human TGF-β2 gene sequence and electrophores TGF-β2 DNA generated by PCR method.

(3) Research result

 1) Measurement of the proliferative effect of hair-forming cells on upgrade-JW12 (see FIG. 5)

  ① The final concentration was treated to 0.05%, 0.1%, and 0.5%. As a result of measuring the cell proliferation rate of HHDPC at 48h, no cytotoxicity was observed at 0.05%, 0.1%, and 0.5% at 48 hours. Cell growth rate of about 20% was observed at the final concentration of 0.05%. Upgrade-JW12 showed proliferative effect in HHDPC.

   ② These results indicate that hair growth promotion effect by upgrade-JW12 has significant activity in preventing / treating hair loss, and thus, it can be considered that it has the possibility of developing hair loss treatment using upgrade-JW12.

 2) Modulation of TGF-β2 Expression, a Parental Growth Cycle Regulatory Protein by Upgrade-JW12

   ① Inhibition of expression of parental cell degeneration phase protein TGF-β2 by upgrade-JW12 in HaCaT cells (see FIG. 6)

Here, Figure 6 (A) is Total RNA from HaCaT treated with upgrade-JW12; Figure 6 (B) is RT-PCR products by specific primer for TGF-β2.

   ② Inhibition of expression of hair growth cell degenerative protein TGF-β2 by upgrade-JW12 in HHDPC cells (see FIG. 7)

Here, Figure 7 (A) is Total RNA from HHDPC treated with upgrade-JW12; Figure 7 (B) is RT-PCR products by specific primer for TGF-β2.

As a result of electrophoresis of RNA isolated from HaCaT and HHDPC in FIGS. 6 and 7, 28S rRNA, 18S rRNA, and 5S rRNA were clearly separated and the same amount was separated through each rRNA band.

     RT-PCR was performed using each group of RNA isolated from each group in HaCaT and HHDPC cells as TGF-β2 primer. The results showed that the expression of TGF-β2 was higher in both HaCaT and HHDPC cells than in the group treated with upgrade-JW12.

    In the HHDPC cell group treated with different concentrations of 0.1% and 1%, the group treated with 0.1% JW had higher expression of TGF-β2 than the group treated with 1% Upgrade-JW12.

     In this way, the cells treated with upgrade-JW12 inhibited the expression of TGF-β2. Therefore, it is thought that the upgrade-JW12 delays the entry of Anagen to Catagen in the hair cycle cycle (see FIG. 8). ).

conclusion

  end. In order to evaluate the efficacy of hair loss prevention and treatment, hair follicle cells were cultured in a culture medium supplemented with upgrade-JW12 in the cytotoxic effect on dermal papilla cells, and then the cytotoxicity was evaluated by MTT method. Evaluation of toxicity and prolifer-ation in hair follicle cells by upgrade-JW12 showed no toxicity in the experimental group of hair follicle cells added 0.05% and 0.1% of upgrade-JW12, and cell growth rate of about 20% was observed in 0.05%. It became.

  I. To evaluate the efficacy of hair follicle growth by upgrade-JW12 in investigating changes in gene expression rates for proteins known to induce degenerative phase (TGF-β2) during hair growth cycle. , TGF-β2 gene expression, which is a protein that induces degeneration, was upgraded by separating total RNA from FW-treated hair follicle cells, and then performing RT-PCR method using TGF-β2 specific primer. In the treated hair follicle cell experimental group, it was observed that the expression level of TGF-β2 was significantly reduced compared to the control group without the upgrade-JW12 treatment.

   All. These results show that cell growth rate and decreased expression of TGF-β2, a protein that induces hair follicle degeneration, in the hair follicle experimental group using the upgrade-JW12 added culture media. It has been shown to be effective.

Experimental Example 2 Photographs of Experienced People with Hair Loss Management Program

10 to 13 is a picture of the hair of the subjects hair growth is promoted through the hair loss prevention program of the present invention. 10 to 13 subjects were successfully hair growth through the hair loss prevention program.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

1 is a process chart showing a method for preparing a copper-peptide-containing low-temperature nano-extracting hair growth promoting agent of the present invention.

Figure 2 is a process chart showing the technical standards of cold nano extraction, separation and concentration products of the present invention.

Figure 3 is a graph showing the change in organic acid according to the storage period of the extract of the present invention.

Figure 4 is a graph showing the change in phenolic acid according to the storage period of the extract of the present invention.

Figure 5 is a graph measuring the proliferation effect of HHDPC hair-forming cells by the hair growth promoter upgrade-JW12 of the present invention.

Figure 6 is an electrophoresis picture showing the inhibition of expression of hair growth cell degenerative induction protein TGF-β2 in HaCaT hair-forming cells by the hair growth promoter upgrade-JW12 of the present invention.

Figure 7 is an electrophoresis picture showing the inhibition of the expression of hair growth cell degenerative induction protein TGF-β2 in HHDPC hair-forming cells by the hair growth promoter upgrade-JW12 of the present invention.

Figure 8 is a photograph showing the regeneration of the hair growth cycle by the hair growth promoter upgrade-JW12 of the present invention.

9 to 12 is a picture of the hair of the subjects hair growth is promoted through the hair loss prevention program of the present invention.

Claims (15)

Copper, including nicotinic acid amide, copper-peptide, salicylic acid, bongyeong extract, lettuce extract, jinjin mugwort extract, seaweed extract, and kelp extract, extracted with nano-scale of 1000 ~ 3000Å at 15 ~ 60 ℃ using mixed solvent of organic solvent and water Peptide-containing low temperature nano extraction hair growth promoter. Low-temperature nano- extraction containing copper-peptide, including nicotinic acid amide, copper-peptide, salicylic acid, Bongyeong extract, lettuce skin extract, jinjin wormwood extract, seaweed extract and kelp extract Hair growth promoter. The method of claim 1 or 2, wherein the peptide of the copper-peptide is alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, A copper-peptide-containing cold nano-extracting hair growth promoter, characterized in that the same or different amino acid residues of any one or a mixture of two or more of leucine, methionine, threonine, tryptophan, tyrosine, valine or arginine are amide-bound peptides. The method of claim 1, wherein the organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof. Copper-peptide-containing low-temperature nano-extraction hair growth promoter to be. According to claim 1 or claim 2, wherein the copper-peptide-containing low-temperature nano-extracting hair growth promoting agent further comprises copper-peptide-containing low-temperature nano-extracted hair growth, characterized in that it further comprises pine needle extract, cypress leaf extract, black soybean extract and right root extract accelerant. According to claim 1 or claim 2, wherein the copper-peptide-containing low-temperature nano hair growth promoting agent further comprises copper-peptide-containing low-temperature nano-extracting hair growth promoter, characterized in that it further comprises a black jima extract, sewage extract, cheongung extract and sulfuric acid extract . According to claim 1 or 2, The content of the nicotinic acid amide is 0.03 to 5 parts by weight, the content of the copper-peptide is 0.05 to 15 parts by weight, the content of the salicylic acid is 0.01 to 5 parts by weight, The content is 0.05 to 10 parts by weight, the content of the extract of the lettuce extract is 0.03 to 8 parts by weight, the content of the jinjin mugwort extract is 0.05 to 10 parts by weight, the content of the seaweed extract is 0.5 to 30 parts by weight and the content of the kelp extract Copper-peptide-containing low-temperature nano-extraction hair growth promoter, characterized in that 0.5 to 30 parts by weight. According to claim 5, wherein the content of the pine needle extract is 0.5 to 10 parts by weight, the content of the cypress leaf extract is 0.5 to 10 parts by weight, the content of the black soybean extract is 0.05 to 8 parts by weight, and the content of the right root extract Copper-peptide-containing low-temperature nano-extraction hair growth promoter, characterized in that 0.03 ~ 5 parts by weight. According to claim 6, wherein the content of the black jima extract is 0.05 to 8 parts by weight, the content of the sewage extract is 0.03 to 5 parts by weight, the content of the cheongung extract is 0.03 to 5 parts by weight, and the content of the sulfuric acid extract is Copper-peptide-containing low-temperature nano-extraction hair growth promoter, characterized in that 0.03 ~ 7 parts by weight. Nicotinic acid amide and copper-peptide were added to the organic solvent, and benzoic acid and lavender oil were sequentially added to salicylic acid to dissolve the main solution (A) in the organic solvent, purified water, and seawater. Boiled the baekbaek leaf, black soybeans, woobangeun, black jima, sewage, cheongung and jihwang, and then extracted with 20 to 50% mixed solvent of the organic solvent and water as an extraction solvent at 15 ~ 60 ℃ for 24 to 48 hours to extract each extract After the preparation, the resultant was filtered with an ultrafilter, again filtered with a nanofilter, separated by centrifugation, placed in a vacuum vacuum concentrator, and then concentrated at a temperature of 30-50 ° C. for 1-5 hours to obtain an organic solvent and water. After evaporation, the copper-peptide-containing low-temperature nano-extracting hair growth promoting agent comprising obtaining a uniform solution of reddish brown after adding to the extraction solution (B) having lowered the concentration of the mixed solvent of organic solvent and water at 0.5-5%. Article methods. Nicotinic acid amide and copper-peptide were added to the purified water, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was added to the purified water and seawater, baekryepi, jinjinmyeong, wakame, kelp, pine needles, white leaf, black soybean. , Boiled, woobangeun, black jima, sewage, cheongung and jihwang boiled and extracted with water as an extraction solvent at 15 ~ 60 ℃ for 24 to 48 hours to prepare each extract, and then filtered with an ultrafilter, and again filtered with a nanofilter Then, the resultant was separated by centrifugation, placed in a vacuum depressurizer, concentrated at a temperature of 30 to 50 ° C. for 1 to 5 hours, added to the evaporated extraction solution (B), and then a reddish brown uniform solution. Copper-peptide-containing low-temperature nano- extraction hair growth promoting agent comprising a obtainable. The peptide of claim 10 or 11, wherein the peptide of the copper-peptide is alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, A method for preparing a copper-peptide-containing cold nano-extracting hair growth promoter, characterized in that the same or different amino acid residues of any one or a mixture of two or more of leucine, methionine, threonine, tryptophan, tyrosine, valine or arginine are amide-bound peptides. The method of claim 10, wherein the organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof. Copper-peptide-containing low-temperature nano-extraction hair growth promoter manufacturing method. According to claim 10 or 11, wherein the content of the nicotinic acid amide is 0.03 to 5 parts by weight, the content of the copper-peptide is 0.05 to 15 parts by weight, the content of the salicylic acid is 0.01 to 5 parts by weight, of the seaweed extract The content is 0.05 to 30 parts by weight, the content of the kelp extract is 0.5 to 30 parts by weight, the content of the pine needle extract is 0.5 to 10 parts by weight, the content of the cypress leaf extract is 0.5 to 10 parts by weight, the content of the black soybean extract Silver 0.05 ~ 8 parts by weight, the content of the right root extract is 0.03 ~ 5 parts by weight, the content of the black jima extract is 0.05 ~ 8 parts by weight, the content of the sewage extract is 0.03 ~ 5 parts by weight, the The content is 0.03 ~ 5 parts by weight, the content of the Bokryeong extract is 0.05 ~ 10 parts by weight, and the content of the sulfuric acid extract is 0.03 ~ 7 parts by weight of copper-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method. 12. The method of claim 10 or 11, wherein the copper-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method is a copper-peptide-containing, characterized in that to perform the screening, washing, drying or crushing of the raw material as a preparation before extraction Low temperature nano extraction hair growth promoter manufacturing method.

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