KR101150374B1 - Hair Growth Promoter Using Low Temperature Nano Extraction Method Having Soy-Oligo-Peptide And Manufacturing Method Thereof - Google Patents

Abstract

The present invention relates to a soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter and a method for producing the same, and more specifically, the soy-oligo-peptide-containing low-temperature nano-extraction hair growth promoting agent using a mixed solvent of an organic solvent and water 15 ~ Soy-oligo-peptide-containing low-temperature nano-hair growth promoter including nicotinic acid amide, soy-oligo-peptide, salicylic acid, Bokryeong extract, lettuce skin extract, jinjin wormwood extract, seaweed extract and kelp extract at 60 ℃ And to a method for producing the same.

In the present invention described above, since the low-temperature nano-extraction method is used, the nano-sized hair growth promoter is well absorbed into hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth. In addition, soy-oligo-peptides, herbal medicines and seaweeds are used as raw materials to prevent various side effects of hormonal therapeutics and overcome shedding. In addition, it contributes to the creation of employment and the local economy, as well as the vitalization of R & D in the bio industry, which can replace the local industry, and the effect of replacing hair loss, wool, hair growth, and hair growth-related products.

Hair Regrowth, Hair Loss Prevention, Wool, Hair Growth, Over Shading, Minerals, Herbal Medicine, Seaweed, Nicotinic Acid Amide, Salicylic Acid, Nano Filter, Low Temperature Extraction, Organic Solvent, Mixed Solvent, Water

Description

Hair Growth Promoter Using Low Temperature Nano Extraction Method Having Soy-Oligo-Peptide And Manufacturing Method Thereof}

The present invention relates to a soy-oligo-peptide-containing low-temperature nano-hair growth promoter and a method for preparing the same, and more particularly, to the soy-oligo-peptide-containing low-temperature nano-extraction hair growth promoter using a mixed solvent or water of an organic solvent and water. Soy-oligo-peptide-containing low temperature nano extraction, including nicotinic acid amide, soy-oligo-peptide, salicylic acid, bokyeong extract, lettuce skin extract, jinjin wormwood extract, seaweed extract and kelp extract at 15-60 ℃ It relates to a hair growth promoting agent and a method of manufacturing the same.

With the development of modern science, human beings are getting better every day and the dream of prolonging their lives is becoming a reality. Nevertheless, the solution of alopecia is still the task of humankind and is being researched and developed in many countries, research institutes and companies. The hair loss prevention and regrowth is a product that has a global marketability.

Human hair has a cosmetic function in addition to functional aspects such as protecting the head from sunlight, absorbing shock from external shock, and maintaining body temperature. In recent years, the number of female hair loss patients has increased due to changes in lifestyles such as westernized eating habits and frequent hair pollution such as perm and dyeing, and adolescent hair loss due to the fact that smoking, academic stress, and puberty itself are accelerated by an average of three years. Patients are also increasing.

Alopecia is a disease that is lost in the area where hair is normally present. It occurs mainly in the head and is classified into non-alopecia areata and alopecia areata according to the damage of hair follicles. No specific drug has been found. Minoxidil has been developed and used as an antihypertensive agent. After Dargie et al. Reported in 1977 the occurrence of hirsutism after oral administration of minoxidil, the topical coating of minoxidil began to be used as a treatment for alopecia.

This has resulted in the release of numerous unproven hair regrowth, hair growth and tonic products worldwide, and the damage caused by unproven products has long been left to consumers suffering from hair loss. Therefore, the development of new hair loss preventing agent and hair growth promoting agent is urgently needed.

In order to solve such a problem, there is known a technique related to a method for manufacturing a hair regrowth using a natural extract and a topical coating agent and a pill (Korean Patent Registration No. 476,731). However, the method of preparing the hair regrowth has a problem that the effect of hair growth is reduced because the natural extract particles acting on the hair root.

Accordingly, an object of the present invention is to solve the problem of side effects caused by conventional unproven hair loss inhibitors and hair growth promoters Soy-oligo-peptides, natural herbal medicines and algae ingredients at low temperature nano-scale hair growth promoter to solve the problem It is an object of the present invention to provide an oligo-peptide-containing low-temperature nano-extraction hair growth promoter and a method for producing the same.

In order to solve the above object, the present invention provides a hair growth promoting agent,

It contains nicotinic acid amide, soy-oligo-peptide, salicylic acid, fueryeong extract, lettuce extract, jinjin worm extract, seaweed extract and kelp extract extracted with nano size of 1000 ~ 3000Å at 15 ~ 60 ℃ using mixed solvent of organic solvent and water. It provides a soy- oligo-peptide-containing low-temperature nano-extracting hair growth promoter.

In addition, the present invention is a hair growth promoter,

Soy-oligo-peptides, including nicotinic acid amide, soy-oligo-peptide, salicylic acid, Bokryeong extract, lettuce extract, jinja worm extract, seaweed extract, and kelp extract It provides a low temperature nano- extraction hair growth promoting agent.

In addition, the present invention is a hair growth promoter manufacturing method,

Nicotinic acid amide and soy-oligo-peptide were added to the organic solvent, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was returned to the organic solvent, purified water, and seawater, and was harvested in the organic solvent, purified water, seaweed, seaweed and kelp Boiled pine needles, cypress leaves, black soybeans, woobangeun, black jima, sewage, cheongung and jihwang and boiled with 20 to 50% mixed solvent of the above-mentioned organic solvent and water extracted for 24 to 48 hours at 15 ~ 60 ℃ each After the extract was prepared, it was filtered through an ultrafilter, again filtered through a nanofilter, separated by centrifugation, placed in a vacuum vacuum concentrator, and concentrated at a temperature of 30 to 50 ° C. for 1 to 5 hours. After evaporating the water, it was added to the extraction solution (B) which lowered the concentration of the mixed solvent of the organic solvent and the water at 0.5 to 5%, and then obtained a soy-oligo-peptide-containing low-temperature nanoparticle containing a reddish brown uniform solution. sinker It provides a method for producing hair growth promoter.

In addition, the present invention is a hair growth promoter manufacturing method,

Nicotinic acid amide and soy-oligo-peptide were added to purified water, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was found in Bokyeong, Sangbaekpi, Injin mugwort, seaweed, seaweed, pine needles and cypress leaves. After boiling, put black soybeans, woobangeun, black jima, sewage, cheongung and jihwang and extract each extract at 15 ~ 60 ℃ for 24 to 48 hours with water as an extraction solvent, and then filtered each with an ultrafilter After filtration through, separated by centrifugation, put into a vacuum vacuum concentrator, concentrated for 1 to 5 hours at a temperature of 30 ~ 50 ℃, water was added to the evaporated extraction solution (B), and then reddish brown uniform It provides a soy-oligo-peptide-containing low-temperature nano- extraction hair growth promoting agent comprising a solution.

Soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter of the present invention and a method for producing the same using low-temperature nano-extraction method, the nano-sized hair growth promoter is well absorbed into hair follicles, the expression of TGF-β2, a hair growth cell degenerative induction protein It is effective in preventing hair loss by inhibiting wool, hair growth, and hair growth. In addition, soy-oligo-peptides, herbal medicines and seaweeds are used as raw materials to prevent various side effects of hormonal therapeutics and overcome shedding. In addition, it contributes to the creation of employment and the local economy, as well as the vitalization of R & D in the bio industry, which can replace the local industry, and the effect of replacing hair loss, wool, hair growth, and hair growth-related products.

The present invention is a soy-oligo-peptide-containing low temperature nano-extracting hair growth promoter, nicotinic acid amide, soy-oligo-peptide, salicylic acid, bokyeong extracted at a nano size of 1000 ~ 3000Å at 15 ~ 60 ℃ using a mixed solvent of organic solvent and water Extracts, lettuce extract, zinnia extract, seaweed extract and kelp extract.

The soy-oligo-peptide-containing cold nano extract hair growth promoter further comprises pine needle extract, cypress leaf extract, black soybean extract and right root extract.

The soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoting agent further comprises a black jima extract, sewage extract, cheongung extract, and sulfuric acid extract.

Here, the organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof.

The soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter was added to the nicotinic acid amide and the soy-oligo-peptide to an organic solvent, and then added to the salicylic acid by sequentially adding benzoic acid and flavoring agent lavender oil (A). 20 of the organic solvent and water extracted from the organic solvent, purified water, and seawater, boiled, and put Bokyeong, Sangbaekpi, Injin mugwort, seaweed, kelp, pine needles, baekbaek leaf, black soybean, woobangeun, black jima, sewage, cheongung and jihwang Each extract was prepared by extracting at 15 to 60 ° C. for 24 to 48 hours with a mixed solvent of 50% to 50%, and then added to the extraction solution (B) having lowered the concentration of the mixed solvent of the organic solvent and water to 0.5 to 5%. The solution is made into a homogeneous solution, separated by centrifugation, filtered through a nanometer, sterilized, and placed in a container. Here, the size of each functional extract is a nano size of 1000 ~ 3000Å. The hair growth promoter is well absorbed by hair follicles and inhibits the expression of TGF-β2, a hair growth cell degenerative protein, to prevent hair loss, and is useful for wool, hair growth, and hair growth. In other words, since the low-temperature nano extraction method is used, the nano-sized hair growth promoter is well absorbed by hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth. In addition, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter overcomes the shedding phenomenon. Shading is a phenomenon in which newly growing healthy hair pushes out resting hair which should be removed anyway, which is a normal process that occurs between two to six weeks after hair loss treatment, and hair falls out more than usual at this time.

At this time, the water may be purified water, distilled water or purified water.

The nicotinic acid amide is one of the water-soluble vitamin B complexes, like nicotinic acid, and exists in vivo as nicotinamide nucleotides that are coenzymes of the vitamin, namely NAD + and NADP +, and is involved in many oxidation and reduction reactions as coenzymes of dehydrogenase. Another function of nicotinamide nucleotides is the reduction of flavincoenzyme, in which case proton addition or desorption is involved in the 4-membered pyridine ring carbon atom. The nicotinic acid amide is hydrolyzed by nicotinamidase and the resulting nicotinic acid is reused for NAD synthesis. In addition, the nicotinic acid amide is methylated in the liver to form n-methylnicotinic acid amide, which is mixed with urine and discharged.

The soy-oligo-peptide is a combination of less than 10 peptides of soybean and is effective for skin regeneration of hair follicles and hair regeneration. The increase in hair follicle size and hair growth rate due to the administration of the soy-oligo-peptide is caused by supplying proper nutrition to the hair follicles through changes in blood flow so that hair with thick hairs grows rapidly. As an example of the soy-oligo-peptide, the soy-oligo-peptide (Soy-Oligo-GHK) whose peptide is Glycyl-Histidyl-Lysine is a very rare sequence extracted from protein in the human body, and collagen, thrombospondin, fibrin a- It is present in a vigorous combination of proteins such as chain, prokininogen, complement C1q, interleukin 4, skin collagenase, coagulation factor XI, and SPARC, which primarily protects tissue and prevents inflammation from tissue damage. It also serves as a signal for tissue reconstruction, regenerating damaged proteins and replacing them with normal tissue. The Soy-Oligo-GHK can be said to be a feedback signal that protects and heals and reorganizes and protects the tissue when the tissue is damaged. In addition, the Soy-Oligo-GHK is excellent skin regeneration ability to promote wound healing, strengthen skin elasticity, and increases the subcutaneous fat layer serves to reverse the aging phenomenon of the skin. In addition, it is recognized as an excellent material in dermatology by increasing the success rate of skin graft surgery, and it increases the hair follicle size as a residual of GHK analogs fat to promote hair growth and reduce hair loss.

Since salicylic acid has the action of antipyretic and analgesic as o-oxybenzoic acid, it is internalized with sodium salt or calcium salt, and is used to improve solubility, stability, pharmacology, etc. by combining salicylic acid with various drugs.

The fukryeong is dried in the fungal nucleus of the intestines to protect the intestines, diuretic and lowering blood sugar, sedation and the like.

The baekbaekpi is a mulberry bark anti-inflammatory action, antioxidant action, skin soothing action, to prevent aging.

The jinjin mugwort is called cheonjae wormwood or Injincho and jaundice, soy sauce, cold cold, anti-inflammatory diuretics, etc. may be taken. The phosphorus mugwort contains dimethylescholetin, scopoline, essential oils, aromatic oxycarboxylic acids, flavonoids, oxycoumarins and the like in the outpost.

The seaweed is rich in dietary fiber and potassium, calcium, iodine, etc., to promote metabolism, postpartum cooking, constipation, obesity prevention, iron and calcium supplements are excellent.

In addition to various pigments such as carotene, xanthophylls, and chlorophyll, the kelp contains a lot of carbohydrates, such as manit and laminarin, which are made by carbon assimilation, and alginic acid, which are components of cell walls, and iodine, vitamin B2, glutamic acid, and the like. Contains amino acids The kelp contains laminin, which lowers blood pressure.

The pine needles are an ingredient of oxyl palmitic acid is effective for skin, beauty and heart strengthening.

The white leaf has a hemostatic action.

In the black soybean, aggregation of platelets is suppressed.

The right root is the root of burdock soothes the sore face and treats sore throat, boil, mange and ringworm. It has been reported that pharmacological action also acts against the various filamentous fungi that cause cancer, activate liver function, purulent bacteria and skin disease.

The black jima is a black sesame to promote the recovery of the skin mucous membranes, greatly reduce the cholesterol level in the blood, and activate the bowel movements.

The sewage is tonic action, hematopoietic function strengthening, fatigue recovery action.

The uterus has a sedation, blood pressure lowering action, antibacterial action.

The sulfur is lowering blood sugar, diuretic, antibacterial action.

The content of the nicotinic acid amide of the hair growth promoter is 0.03 to 5 parts by weight, the content of the soy-oligo-peptide is 0.05 to 25 parts by weight, the content of the salicylic acid is 0.01 to 5 parts by weight, and the content of the Bokryeong extract is 0.05 to 10 parts by weight, the content of the lettuce extract is 0.03 to 8 parts by weight, the content of the jinjin mugwort extract is 0.05 to 10 parts by weight, the content of the seaweed extract is 0.5 to 30 parts by weight and the content of the kelp extract is 0.5 to 30 weight It is wealth.

When the content of each component is less than the above range, it is difficult to exhibit hair growth, and when the content of each of the above components exceeds the above range, it may exhibit side effects.

In addition, the content of the pine needle extract of the hair growth promoter is 0.5 to 10 parts by weight, the content of the cypress leaf extract is 0.5 to 10 parts by weight, the content of the black soybean extract is 0.05 to 8 parts by weight, and the content of woobangeun extract 0.03 to 5 parts by weight.

When the content of each of the components is less than the above range, it is difficult to exhibit hair growth and skin soothing action, and when the content of each of the above components exceeds the range, side effects may be exhibited.

In addition, the content of the black jima extract of the hair growth promoter is 0.05 to 8 parts by weight, the content of the sewage extract is 0.03 to 5 parts by weight, the content of the cheongung extract is 0.03 to 5 parts by weight, and the content of the sulfuric acid extract is 0.03-7 weight part.

When the content of each component is less than the above range, it is difficult to exhibit hair growth and skin pharmacological action, and when the content of each of the above components exceeds the range, side effects may be exhibited.

In addition, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter of the present invention, nicotinic acid amide, soy-oligo-peptide, salicylic acid, bokyeong extract, lettuce extract Extracts, rhizome extracts, seaweed extracts, and kelp extracts.

Here, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter is added to the nicotinic acid amide and the soy-oligo-peptide to purified water, and added to the salicylic acid in order to dissolve the benzoic acid and flavoring agent lavender (oil) A) boiled in purified water and sea water, put Bokyeong, Sangbaekpi, Injin mugwort, seaweed, kelp, pine needles, baekbaek leaf, black soybean, woobangeun, black jima, sewage, cheongung and jihwang, and then cooled and extracted with water as an extraction solvent for 24 to 48 hours. After extracting at 15 ~ 60 ℃ for preparing each extract, water was added to the evaporated extraction solution (B), then made into a uniform solution separated by centrifugation, filtered by nanometer and sterilized It is in. At this time, the size of each functional extract is a nano size of 1000 ~ 3000Å. The hair growth promoter is well absorbed by hair follicles and inhibits the expression of TGF-β2, a hair growth cell degenerative protein, to prevent hair loss, and is useful for wool, hair growth, and hair growth. In other words, since the low-temperature nano extraction method is used, the nano-sized hair growth promoter is well absorbed by hair follicles and is useful for hair loss prevention, wool, hair growth, and hair growth. In addition, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter overcomes the shedding phenomenon.

At this time, the water may be purified water, distilled water or purified water.

In addition, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method of the present invention,

Nicotinic acid amide and soy-oligo-peptide were added to the organic solvent, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was returned to the organic solvent, purified water, and seawater, and was harvested in the organic solvent, purified water, seaweed, seaweed and kelp Boiled pine needles, cypress leaves, black soybeans, woobangeun, black jima, sewage, cheongung and jihwang and boiled with 20 to 50% mixed solvent of the above-mentioned organic solvent and water extracted for 24 to 48 hours at 15 ~ 60 ℃ each After the extract was prepared, it was filtered through an ultrafilter, again filtered through a nanofilter, separated by centrifugation, placed in a vacuum vacuum concentrator, and concentrated at a temperature of 30 to 50 ° C. for 1 to 5 hours. After evaporating the water, it is added to the extraction solution (B) having lowered the concentration of the mixed solvent of the organic solvent and water at 0.5 to 5%, to obtain a reddish brown uniform solution.

At this time, the peptide of the soy-oligo-peptide is alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, aspartate, isoleucine, leucine, methionine, threonine, The same or different amino acid residues, either one of tryptophan, tyrosine, valine or arginine, or a mixture of two or more thereof, are amide linked peptides.

Here, the organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin or a mixture of two or more thereof.

At this time, the water may be purified water, distilled water or purified water.

In addition, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method of the present invention,

Nicotinic acid amide and soy-oligo-peptide were added to purified water, and the main solution (A) dissolved by sequentially adding benzoic acid and lavender oil to salicylic acid was found in Bokyeong, Sangbaekpi, Injin mugwort, seaweed, seaweed, pine needles and cypress leaves. After boiling, put black soybeans, woobangeun, black jima, sewage, cheongung and jihwang and extract each extract at 15 ~ 60 ℃ for 24 to 48 hours with water as an extraction solvent, and then filtered each with an ultrafilter After filtration through, separated by centrifugation, put into a vacuum vacuum concentrator, concentrated for 1 to 5 hours at a temperature of 30 ~ 50 ℃, water was added to the evaporated extraction solution (B), and then reddish brown uniform To get a solution.

At this time, the water may be purified water, distilled water or purified water.

Here, the content of the nicotinic acid amide is 0.03 to 5 parts by weight, the content of the soy-oligo-peptide is 0.05 to 25 parts by weight, the content of the salicylic acid is 0.01 to 5 parts by weight, the content of the seaweed extract is 0.05 to 30 weight The content of the kelp extract is 0.5 to 30 parts by weight, the content of the pine needle extract is 0.5 to 10 parts by weight, the content of the cypress leaf extract is 0.5 to 10 parts by weight, and the content of the black soybean extract is 0.05 to 8 parts by weight. , The content of the right root extract is 0.03 ~ 5 parts by weight, the content of the black jima extract is 0.05 ~ 8 parts by weight, the content of the sewage extract is 0.03 ~ 5 parts by weight, the content of the cheongung extract is 0.03 ~ 5 parts by weight , The content of the Bokryeong extract is 0.05 to 10 parts by weight, and the content of the turmeric extract is 0.03 to 7 parts by weight.

The above description of each extract and the content of each extract uses the preceding description.

In addition, the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method is a pre-extraction preparation to perform the selection, washing, drying or crushing of the raw materials.

Here, the soy-oligo-peptide-containing low-temperature nano-hair extraction promoter manufacturing method by applying a step-by-step low-temperature nano- extraction method to the object by using ultrasonic waves after the screening, washing process using natural herbal ingredients and seaweeds in the production of hair loss prevention products When extracting raw materials, it can be manufactured in such a way as to maximize its effectiveness and extraction of specific components.

In addition, the soy-oligo-peptide-containing low-temperature nano-extraction hair growth promoter manufacturing method will be described with reference to the drawings.

1 is a process chart showing a method for preparing a soy-oligo-peptide-containing low-temperature nano-extraction hair growth promoter of the present invention.

As shown in Figure 1, after preparing soy-oligo-peptides, algae, natural herbal medicines, herbal medicines, and other materials, the preparation work before extraction, such as sorting, washing, drying, crushing.

Then, each raw material is extracted for each raw material extracted with a mixed solvent of organic solvent and water or purified water.

After extracting the raw materials, the ultrafiltration process is performed, and the extract which is difficult to be separated by normal filtration is filtered by using dialysis paper using a sulfate sheet, a bladder membrane, and colloidion.

Thereafter, a nanofiltration process is performed using a nanofilter on the extract obtained by the ultrafiltration process.

Then, the resultant is separated by centrifugation, placed in a vacuum vacuum concentrator and concentrated, and then the organic solvent or water is evaporated to lower the alcohol concentration to produce an extract (B).

In the case of extraction with purified water, the purified water is evaporated to generate an extraction solution (B).

Thereafter, nicotinic acid amide and soy-oligo-peptide are added to the organic solvent or purified water, and benzoic acid and lavender oil are sequentially added to salicylic acid and added to the dissolved main solution (A). The product is then produced as a reddish brown homogeneous solution.

In addition, the hair loss management system of the present invention consultation and shooting; Primary sampu; Scalp steamer; Scalp peeling and oxygen supply; Infrared irradiation; Ampoule application; Scalp massage; And secondary sampu; sequentially.

In the counseling and photographing step, when a baldness visits, a person consults about eating habits and hair hygiene, and photographs a bald area with a digital camera. At this time, shoot with the hair enlarger.

In the first sampu step to remove the first contaminants such as foreign substances by scalp cleaning before management.

The scalp steamer step facilitates the removal of fine contaminants remaining in the scalp (mogu) by steam and absorption of the hair growth promoter ampoule.

In the scalp peeling and oxygen supplying step, oxygen is supplied to the scalp and contaminant filling and scalp in the scalp (mogu) relaxed with steam.

In the infrared irradiation step helps to circulate blood using infrared rays.

In the ampoule application step, the hair growth promoter ampoule for nourishing the scalp is finely sprayed with an oxygen jet spray.

In the scalp massage step to massage the scalp to improve the blood circulation of the scalp to increase the absorption of the hair growth promoter ampoule.

Finally, in the second shampoo phase, the final shampoo after absorbing the hair growth promoter ampoule creates a personal style.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

EXAMPLES Material Extraction and Methods

After sorting each material (natural herbal medicine and algae), it is prepared by washing, drying, crushing, etc. for detailed selective extraction according to the functional material of each raw material. It was made to include the extract. The extracted active ingredients are again subjected to primary extraction and concentration using ultrafiltration (ultrafiltration, MWCO, 20,000), followed by extraction and concentration according to molecular weight in the second order through nanofiltration (nanofiltration, MWCO, 10,000). As an example of the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter (product name: upgrade-JW12) was prepared (see Fig. 2).

Soy- oligo-peptide-containing low-temperature nano-extracting hair growth promoter (product name: upgrade-JW12) of the present invention the composition and content of the extract is as follows.

The content of nicotinic acid amide is 0.1g, the content of soy-oligo-peptide is 1g, the content of salicylic acid is 0.1g, the content of seaweed extract is 3g, the content of seaweed extract is 4g, the content of pine needle extract is 2.5g, the cypress leaf extract The content of 2.5g, the content of black soybean extract 1g, the content of woobangeun extract 0.5g, the content of black tide extract 1g, the content of sewage extract 0.5g, the content of cheongung extract 0.3g, the content of Bokryeong extract 0.3g of silver, 0.8g of lettuce extract, 0.5g of phosphorus extract, and 0.3g of turmeric extract, 0.2g of benzoic acid as a preservative, 0.1g of lavender oil as a flavoring agent, Add 30 g of ethanol and the rest with purified water and sea water.

Experimental Example 1

Content of experiment

end. Establish standardization of extraction, separation, purification and concentration of functional ingredients from seaweeds and natural herbs through low temperature nano extraction

(1) In order to prevent hair loss and maximize the effects of wool and hair growth, functional bioactive substances that require specific heat treatment of raw materials were extracted and obtained at 50 ° C or below.

(2) Functional bioactive materials requiring organic solvent extraction were extracted from 50% organic solvent.

(3) Natural herbal medicines were separated / filtered secondly after the first hot water extraction.

(4) The concentration of the extract was tested for efficacy at 25% or 50% concentration (water and alcohol removal) using a general vacuum evaporation at 40 ° C.

I. Analysis of General Components of Extracts

Component analysis was conducted in 1st (2006/08/25), 2nd (2006/10/16), 3rd (2006/12/16), and 4th (2007/02/17).

(1) Analysis

 1) Elemental Analysis

ICP (Inductively Coupled Plasma Emission Spectrometer, Thermo Jarrel Ash Co., IRIS / AP) was used to investigate the elemental content and content of the sample. Sample (3 g) was added H ₂ O ₂ 2 to HN ₃ 10 and digested with Microwave (Malstone Co.,) and filled up with standard amount 20 with 2-3% HNO ₃ .

 2) Free Sugar Analysis

   HPLC (High Performance Liquid Chromatography, Waters Co., Sugarpak Icolumn) was used to investigate the free sugar component and content of the samples.

   Sample 1 was filtered through a microfilter and used as the sample.

 3) amino acid analysis

   An automatic amino acid analyzer (Automatic Amio Acid Analyzer, Pharmacia Biotech Co., Biochrom 20, Na form column) was used to investigate the amino acid content and content of the sample.

   The sample was hydrolyzed with 6N hydrochloric acid (HCl), filtered through a microfilter, and used as a sample.

 4) Microbial Analysis

   In order to analyze the microorganisms in the sample, the presence of Escherichia coli, bacteria, actinomycetes, yeast, and fungi was analyzed by the general Bergy's mannul method and 3M Petrifilm.

(2) Analysis result

 1) Elemental Analysis (ppm) of the product (Upgrade-JW12; Hair Growth Promoter)

Element JW0825 JW1016 JW1216 JW0217 K 1453.5 1776.9 1755.2 1507.3 Ca 465.6 483.6 504.5 406.8 Mg 728.5 805.5 759.6 1004.6 P 105.4 126.7 134.2 90.5 B 16.3 26.7 13.9 0 Mo 0.3 0.2 0.3 0.6 Si 9.9 11.6 10.3 8.5 Fe 6.9 5.8 7.1 8.2 Mn 9.6 10.3 10.8 9.3 Cu 0.6 0.5 0.4 0.7 CD 0 0 0 0 Cr 2 2.1 2.2 2.2 Pb 0 0 0 0 Zn 0.5 0.4 0.2 0 Ni 1.7 1.9 1.8 1.7 Ti 0 0 0 0 Al 0.4 0.3 0.3 0

2) Analysis of free sugar (%) of the product (upgrade-JW12; hair growth promoter)

Name JW0825 JW1016 JW1216 JW0217 Glucose 9.95 8.66 9.59 9.11 Sucrose 13.27 13.79 10.23 12.50 Fructose 10.56 11.32 13.26 14.55 Maltose 0.00 0.00 0.00 0.00 Lactose 0.00 0.00 0.00 0.00

3) Microbiological analysis (cfu / ml) of the product (Upgrade-JW12; hair growth promoter)

Microorganism name JW0825 JW1016 JW1216 JW0217 Escherichia coli 0.00 0.00 0.00 0.00 General bacterial count 0.00 0.00 0.00 0.00 Actinomycetes 0.00 0.00 0.00 0.00 Yeast 0.00 0.00 0.00 0.00 Mold water 0.00 0.00 0.00 0.00

4) Amino acid analysis (ppm) of the product (Upgrade-JW12; hair growth promoter)

No. Name JW0825 JW1016 JW1216 JW0217 One Asp 155.09 100.59 146.49 130.27 2 Thr 32.23 23.35 22.98 20.32 3 Ser 28.44 27.24 22.09 20.61 4 Glu 274.48 197.77 242.21 240.09 5 Pro 42.00 32.56 31.53 31.42 6 Gly 735.25 823.33 636.23 728.50 7 Ala 55.13 36.89 0.00 5.43 8 Cys 41.46 19.68 22.90 19.35 9 Val 0.00 26.54 4.56 14.25 10 Met 0.00 0.00 0.00 0.00 11 Ile 17.86 16.56 23.43 19.36 12 Leu 26.66 15.86 24.16 25.23 13 Tyr 0.00 0.00 16.69 14.32 14 Phe 18.15 10.67 0.00 0.00 15 His 500.68 657.42 589.00 673.00 16 Lys 840.99 827.78 733.67 631.46 17 Amm 168.54 146.00 156.96 160.69 18 Arg 0.00 0.00 0.00 0.00

5) Catechin analysis of product (upgrade-JW12; hair growth promoter) (unit: mg%)

(-) EGC (+) C (-) EC (-) EGCG (-) ECG 66.359 3.157 3.984 9.224 0.939

6) Vitamin component of the product (Upgrade-JW12; hair growth promoter) (Unit: mg / 100g)

Vitamin b1 Vitamin b2 Vitamin b6 Vitamin c 0.148 16.226 3.439 5.887

All. Long term preservation test of extract

Upgrade-JW12 is divided into 1st (2007/02), 2nd (2007/04), 3rd (2007/06), and 4th (2007/08) experiments to change the organic and phenolic acid components at two-month intervals. (Table 7, FIG. 3; Table 8, FIG. 4).

 (1) Change of organic acid according to storage period

Storage time
(Month) Organic acid (Unit: mg%) Oxalic Citric Malic Malonic Succinic Fumaric Upgrade-JW12-02February 25.25 387.22 254.46 59.78 884.97 0.52 Upgrade-JW12-04 25.38 304.69 249.99 58.99 1432.37 0.66 Upgrade-JW12-June 25.17 305.88 252.04 58.28 1429.55 0.65 Upgrade-JW12-08 25.11 317.93 245.64 79.86 1469.23 0.59

(2) Phenolic Acid Changes According to Storage Period

Storage time
(Month) Phenolics (Unit: mg%) Caffeic Chlorogenic p-coumaric Salicylic Ferulic Cinamic Upgrade-JW12-02February 5.43 1.01 0.89 0.83 1.70 0.12 Upgrade-JW12-04 9.56 1.29 1.03 1.01 1.69 0.10 Upgrade-JW12-June 9.88 1.43 1.04 1.05 1.62 0.11 Upgrade-JW12-08 9.89 1.42 1.03 1.05 1.62 0.10

la. Consignment institution (Department of Oriental Medical Materials Engineering, Kyung Hee University)

(1) Measurement of proliferative effect of hair-forming cells on upgrade-JW12

  1) Experimental material

    Human hair follicle cells, human dermal papilla cells (HHDPC) were used as hair-forming cells, and cell culture medium was cultured using MSC and subcultured once every 3 days.

2) Sample Preparation

As a test substance, the concentration of the natural product extract, Upgrade-JW12, was used at a final concentration of 0.05%, 0.1%, and 0.5%, and H ₂ O was used as a negative control.

3) Cell growth rate measurement

    Proliferation evaluation as follows by quantifying the formation of formazan pigment by living mitochondria by 3- [4,5-dimethylthiazol-2-yl] -2 and 5-diphenyltetraolium bromide test (MTT) method Was carried out.

① The HHDPC cells were adjusted to an initial cell concentration of about 1 × 10 ⁴ cells / well per well using a 96-hole plate incubator.

② The treatment of the sample (Upgrade-JW12) was dissolved in H ₂ O as a solvent, the serum content was adjusted to 5% concentration, and then diluted sequentially with MSC medium.

③ The medium was adjusted to an amount of about 200ml per well and incubated in 37 ° C. and 5% CO ₂ incubator for 3 days.

   ④ 50 ml of the MTT solution prepared at a concentration of 2 mg / ml was added per well, and left for 4 hours in the same incubator.

   ⑤ When the MTT reagent was determined to be absorbed into the intracellular mitochondria, the supernatant was removed, 150 ml of DMSO was added, and stirred for 10 minutes to uniformly dissolve formazan dye.

   ⑥ After that, the absorbance value was measured at 540 nm using an ELISA reader, and the cell number was measured according to a standard sample curve prepared in advance.

 (2) Modulation of TGF-β2 Expression, a Parental Growth Cycle Regulatory Protein by Upgrade-JW12

  1) Experimental material

   ① Human keratinocyte HaCaT was used as hair-forming cells, and cell culture medium was cultured using RPMI and subcultured once every 3 days.

   ② Human hair follicle cells, human dermal papilla cells (HHDPC) were used, and cell culture medium was cultured using MSC, and passaged once every 3 days.

2) Preparation of Sample

    As a test substance, a natural product extract, Upgrade-JW12, was used, and a final concentration of 0.1% or 1% was used.

3) Measurement of TGF-β2 Expression

① Incubate HaCaT and HHDPC in 60mm dish and incubate for 24 hours in 0.5% FBS condition medium when each cell shows 70% confluency. Incubate for 24 hours, and set untreated group (-) as a control, and harvest each cell with TRIzol reagent after 24 hours incubation.

  ② Isolate total RNA from each cell by chromoform extraction and isopropanol precipitation method and quantify RNA.

  ③ Synthesize TGF-β2 cDNA using reverse transcriptase from total RNA isolated using TGF-β2 specific primer using human TGF-β2 gene sequence and electrophores TGF-β2 DNA generated by PCR method.

(3) Research result

 1) Measurement of the proliferative effect of hair-forming cells on upgrade-JW12 (see FIG. 5)

  ① The final concentration was treated to 0.05%, 0.1%, and 0.5%. As a result of measuring the cell proliferation rate of HHDPC at 48h, no cytotoxicity was observed at 0.05%, 0.1%, and 0.5% at 48 hours. Cell growth rate of about 21% was observed at the final concentration of 0.05%, indicating that upgrade-JW12 showed proliferative effect in HHDPC.

   ② These results indicate that hair growth promotion effect by upgrade-JW12 has significant activity in preventing / treating hair loss, and thus, it can be considered that it has the possibility of developing hair loss treatment using upgrade-JW12.

 2) Modulation of TGF-β2 Expression, a Parental Growth Cycle Regulatory Protein by Upgrade-JW12

   ① Inhibition of expression of parental cell degeneration phase protein TGF-β2 by upgrade-JW12 in HaCaT cells (see FIG. 6)

Here, Figure 6 (A) is Total RNA from HaCaT treated with upgrade-JW12; Figure 6 (B) is RT-PCR products by specific primer for TGF-β2.

   ② Inhibition of expression of hair growth cell degenerative protein TGF-β2 by upgrade-JW12 in HHDPC cells (see FIG. 7)

Here, Figure 7 (A) is Total RNA from HHDPC treated with upgrade-JW12; Figure 7 (B) is RT-PCR products by specific primer for TGF-β2.

As a result of electrophoresis of RNA isolated from HaCaT and HHDPC in FIGS. 6 and 7, 28S rRNA, 18S rRNA, and 5S rRNA were clearly separated and the same amount was separated through each rRNA band.

     RT-PCR was performed using each group of RNA isolated from each group in HaCaT and HHDPC cells as TGF-β2 primer. The results showed that the expression of TGF-β2 was higher in both HaCaT and HHDPC cells than in the group treated with upgrade-JW12.

    In the HHDPC cell group treated with different concentrations of 0.1% and 1%, the group treated with 0.1% JW had higher expression of TGF-β2 than the group treated with 1% Upgrade-JW12.

     In this way, the cells treated with upgrade-JW12 inhibited the expression of TGF-β2. Therefore, it is thought that the upgrade-JW12 delays the entry of Anagen to Catagen in the hair cycle cycle (see FIG. 8). ).

conclusion

  end. In order to evaluate the efficacy of hair loss prevention and treatment, hair follicle cells were cultured in a culture medium supplemented with upgrade-JW12 in the cytotoxic effect on dermal papilla cells, and then the cytotoxicity was evaluated by MTT method. Evaluation of toxicity and prolifer-ation in hair follicle cells by upgrade-JW12 showed no toxicity in the experimental group of hair follicle cells with 0.05% and 0.1% of upgrade-JW12, and cell growth rate of about 21% was observed in 0.05%. It became.

  I. To evaluate the efficacy of hair follicle growth by upgrade-JW12 in investigating changes in gene expression rates for proteins known to induce degenerative phase (TGF-β2) during hair growth cycle. , TGF-β2 gene expression, which is a protein that induces degeneration, was upgraded by separating total RNA from FW-treated hair follicle cells, and then performing RT-PCR method using TGF-β2 specific primer. In the treated hair follicle cell experimental group, it was observed that the expression level of TGF-β2 was significantly reduced compared to the control group without the upgrade-JW12 treatment.

   All. These results show that cell growth rate and decreased expression of TGF-β2, a protein that induces hair follicle degeneration, in the hair follicle experimental group using the upgrade-JW12 added culture media. It has been shown to be effective.

Experimental Example 2 Photographs of Experienced People with Hair Loss Management Program

9 to 11 is a picture of the hair of the subjects hair growth is promoted through the hair loss prevention program of the present invention. 9 to 11 subjects were successfully hair growth through the hair loss prevention program.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

1 is a process chart showing a method for preparing a soy-oligo-peptide-containing low-temperature nano-extraction hair growth promoter of the present invention.

Figure 2 is a process chart showing the technical standards of cold nano extraction, separation and concentration products of the present invention.

Figure 3 is a graph showing the change in organic acid according to the storage period of the extract of the present invention.

Figure 4 is a graph showing the change in phenolic acid according to the storage period of the extract of the present invention.

Figure 5 is a graph measuring the proliferation effect of HHDPC hair-forming cells by the hair growth promoter upgrade-JW12 of the present invention.

Figure 6 is an electrophoresis picture showing the inhibition of expression of hair growth cell degenerative induction protein TGF-β2 in HaCaT hair-forming cells by the hair growth promoter upgrade-JW12 of the present invention.

Figure 7 is an electrophoresis picture showing the inhibition of the expression of hair growth cell degenerative induction protein TGF-β2 in HHDPC hair-forming cells by the hair growth promoter upgrade-JW12 of the present invention.

Figure 8 is a photograph showing the regeneration of the hair growth cycle by the hair growth promoter upgrade-JW12 of the present invention.

9 to 11 is a picture of the hair of the subjects hair growth is promoted through the hair loss prevention program of the present invention.

Claims (15)

0.03 to 5 parts by weight of nicotinic acid amide extracted at a nano size of 1000 to 3000 mm 3 at 15 to 60 ° C. using an organic solvent of 40 to 60 wt%; Alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, leucine, methionine, threonine, tryptophan, tyrosine, valine or arginine 0.05 to 25 parts by weight of a soy-oligo-peptide, characterized in that the same or different amino acid residues, which are mixtures of two or more thereof, are amide linked peptides; 0.01-5 parts by weight of salicylic acid; 0.05 ~ 10 parts by weight of the bokyong extract; 0.03-8 parts by weight of lettuce extract; Phosphorus mugwort extract 0.05 ~ 10 parts by weight; 0.5 to 30 parts by weight of seaweed extract; And Contains 0.5 to 30 parts by weight of kelp extract, The organic solvent is soy- oligo-peptide-containing cold nano extraction comprising at least one selected from methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, and glycerin Hair growth promoter. 0.03 to 5 parts by weight of nicotinic acid amide extracted at a nanosize of 1000 to 3000 mm 3 at 15 to 60 ° C. with water; Alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, leucine, methionine, threonine, tryptophan, tyrosine, valine or arginine 0.05 to 25 parts by weight of a soy-oligo-peptide, characterized in that the same or different amino acid residues, which are mixtures of two or more thereof, are amide-bound peptides; 0.01-5 parts by weight of salicylic acid; 0.05 ~ 10 parts by weight of the bokyong extract; 0.03-8 parts by weight of lettuce extract; Phosphorus mugwort extract 0.05 ~ 10 parts by weight; 0.5 to 30 parts by weight of seaweed extract; And Soy-oligo-peptide-containing low-temperature nano-hair growth promoter containing 0.5 to 30 parts by weight of kelp extract. delete delete According to claim 1 or 2, wherein the Soy- oligo-peptide-containing low-temperature nano-extracting hair growth promoter 0.5 to 10 parts by weight of pine needle extract, 0.5 to 10 parts by weight of cypress leaf extract, 0.05 to 8 parts by weight of black soybean extract and right root Soy-oligo-peptide-containing low-temperature nano-hair growth promoter, characterized in that it further comprises 0.03 ~ 5 parts by weight of the extract. According to claim 1 or 2, wherein the Soy- oligo-peptide-containing low-temperature nano-extracting hair growth promoting agent 0.05 ~ 8 parts by weight of black jima extract, 0.03 ~ 5 parts by weight of sewage extract, 0.03 ~ 5 parts by weight of cheongung extract and turmeric extract Soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter, characterized in that it further comprises 0.03 ~ 7 parts by weight. delete delete delete 0.03 to 5 parts by weight of nicotinic acid amide in an organic solvent of 40 to 60% by weight and Alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, leucine, methionine, threonine, tryptophan, tyrosine, valine or arginine 0.05-15 parts by weight of copper-peptide, characterized in that the same or different amino acid residues, which are mixtures of two or more of them, are amide-linked peptides, and 0.011-1.5 parts by weight of benzoic acid and 0.01-1 parts by weight of lavender oil to 0.01-1 part by weight of salicylic acid. 1.0 parts by weight of the main solution (A) Fukryeong 0.05 ~ 10 parts by weight, Sangbaekpi 0.03 ~ 8 parts by weight, 0.05 ~ 10 parts by weight, seaweed 0.5-30 parts by weight, seaweed 0.5-30 parts by weight, seaweed 0.5-30 parts by weight, pine needles 0.5-10 parts by weight in the organic solvent, purified water and sea water, 0.5 ~ 10 parts by weight, black soybean 0.05 ~ 8 parts by weight, right roots 0.03 ~ 5 parts by weight, black zebra0.05 ~ 8 parts by weight, sewage 0.03 ~ 5 parts by weight, uterus 0.03 ~ 5 parts by weight and turmeric 0.03 ~ 7 parts by weight After boiling, the mixture was extracted with 20 to 50 wt% mixed solvent of the organic solvent and water, which were extracted at 15 to 60 ° C. for 24 to 48 hours to prepare each extract, and then filtered with an ultrafilter and then filtered with a nanofilter. After that, the mixture was separated by centrifugation, placed in a vacuum vacuum concentrator, and concentrated at a temperature of 30 to 50 ° C. for 1 to 5 hours to evaporate the organic solvent and water, followed by 0.5 to 5 wt% of the organic solvent and water. After adding to the extraction solution (B) with a lower concentration of the mixed solvent, to obtain a reddish brown uniform solution, The organic solvent is any one of methanol, ethanol, ether, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin, or a mixture of two or more thereof. Soy-oligo-peptide-containing low-temperature nano extraction hair growth promoter manufacturing method. 0.03-5 parts by weight of nicotinic acid amide in purified water and Alanine, glycine, lysine, histidine, serine, proline, hydroxyproline, threonine, glutamic acid, methionine, glutamine, phenylalanine, asphaltene, isoleucine, leucine, methionine, threonine, tryptophan, tyrosine, valine or arginine 0.05-15 parts by weight of copper-peptide, characterized in that the same or different amino acid residues, which are mixtures of two or more of them, are amide-bound peptides, 0.01-1.5 parts by weight of benzoic acid and 0.01-1.5 parts by weight of benzoic acid, to 0.01 to 5 parts by weight of salicylic acid. 10 parts by weight of the main solution (A) To the purified water and seawater Fukryeong 0.05 ~ 10 parts by weight, baekbaekpi 0.03 ~ 8 parts by weight, phosphorus mugwort 0.05 ~ 10 parts by weight, seaweed 0.5 ~ 30 parts by weight, kelp 0.5 ~ 30 parts by weight, pine needles 0.5 ~ 10 parts by weight, cypress leaves 0.5 ~ 10 parts by weight, 0.05-8 parts by weight of black soybean, 0.03-5 parts by weight of right root, 0.05-8 parts by weight of black horse, 0.03-5 parts by weight of sewage, 0.03-5 parts by weight of archery, and 0.03-7 parts by weight of turmeric After extracting each extract by extracting at 15 ~ 60 ℃ for 24 to 48 hours with water as an extraction solvent, and then filtered with an ultrafilter, and again filtered with a nanofilter, separated by centrifugation, put into a vacuum decompression concentrator After concentrating for 1 to 5 hours at a temperature of 30 ~ 50 ℃ and added to the evaporated extract solution (B), soy- oligo-peptide-containing low-temperature nano-extracting hair growth promoter comprising obtaining a reddish brown uniform solution Manufacturing method. delete delete delete 12. The method of claim 10 or 11, wherein the soy-oligo-peptide-containing low-temperature nano-extracting hair growth promoter manufacturing method is a pre-extraction preparation, characterized in that the selection, washing, drying or crushing of the raw material to perform the soy- Oligo-peptide-containing low-temperature nano extraction hair growth promoter manufacturing method.

Images