KR102068523B1 - Composition for alleviating hair loss and promoting hair growth using sun-salt - Google Patents

Abstract

The present invention relates to a composition for alleviating hair loss and promoting hair growth using sun salt, and by containing a large amount of sun salt to alleviate inflammation of the skin and scalp through the removal of waste, moisturizing, sterilization, anti-inflammatory, and blood circulation, which are the basic functions of salt. Efficiently remove wastes and efficiently supply nutrients needed for scalp and hair through the composition of spinach extract, kelp extract, and yellow lacquer extract to prevent the cause of hair loss and alleviate the symptoms of hair loss The present invention relates to a hair loss alleviation and hair growth promoting composition using sun salt, which is effective to produce healthy and rich hair at the same time.

Description

Composition for alleviating hair loss and promoting hair growth using sun-salt}

The present invention relates to a hair loss alleviation and hair growth promoting composition using sun salt, and to a hair loss relaxation and hair growth promoting composition using sun salt containing sun salt, spinach extract, kelp extract, and yellow lacquer extract. More specifically, by containing a large amount of sun salt, it removes waste, moisturizes, sterilizes, anti-inflammatory and promotes blood circulation, which is the basic function of salt, to relieve inflammation of the skin and scalp, and effectively removes waste, while spinach extract, By efficiently supplying the nutrients needed by the scalp and hair through compositions such as kelp extract and yellow lacquer extract, sun salt prevents the cause of hair loss, alleviates the symptoms of hair loss, and helps to produce healthy and rich hair. The present invention relates to a composition for alleviating hair loss and promoting hair growth.

In general, hair loss symptoms can be classified into genetic and acquired factors, and the causes include mental stress, hormonal imbalance, skin damage caused by chemicals, diseases with high fever, physical scalp damage, and skin. It is caused by various factors such as infection, aging, excessive application of chemicals to hair (firms, dyes, etc.), sunlight damage, and malnutrition.

This hair loss phenomenon has been increasing rapidly in recent years, and about 20% of adult males are not related to hair loss. According to the National Institute of Health Management, it is estimated that the domestic hair loss population will reach 10 million. In particular, in view of the rapid increase in the proportion of 2030 young and female patients, stress, drinking, irregular sleep time, childbirth, excessive diet, frequent perming, dyeing, mousse, and spray, in addition to the causes of genetic or disease and aging. The proportion of hair loss is increasing due to various acquired factors, such as excessive use, and contamination of the scalp and hair due to a sudden increase in environmental pollution, air pollution, especially fine dust, and inflammation.

In addition, one of the causes of hair loss is not related to the diet of modern people. As the consumption of instant foods increases, modern people lack minerals and minerals, and hair loss due to nutritional imbalance cannot be overlooked.

Hair loss is not a life-threatening disease, but it can cause mental anxiety and stress, causing a variety of diseases. Moreover, it can cause inferiority to modern people who have high interest and expectation of appearance, and it causes a lot of inconvenience to daily life by losing confidence and shrinking.

Therefore, hair loss treatment and symptom improvement not only make scalp health and abundant hair, but also satisfy the aesthetic needs of modern people, thereby increasing confidence and self-esteem, helping to lead a more satisfying and happy life. For these reasons, the development of compositions and products that help hair growth and alleviate hair loss is an important task required in modern society.

For these reasons, a number of studies on hair loss and hair growth have been conducted, and these studies have led to some causes of acquired causes and suggested ways to improve them.

Looking at the existing prior patents and research, "Painted scrub cosmetic composition using the sun salt" of the Patent Publication No. 10-2016-0139993 to try to help the hair loss relief through the scrub composition containing the spinach in the sun salt. -1797667 "Composition for the prevention, improvement or treatment of hair loss and a method for preparing the same" has developed a hair loss alleviating composition by containing herbs and salt in laver, sewage, green tea leaves, and "Prevent hair loss of Patent No. 10-2005-0102346 And cosmetic composition having a hair growth promoting effect "by using a composition containing Samsam (沙 蔘, Adenophora triphylla) extract to activate the hair follicles, help to promote hair growth to improve the hair loss symptoms.

In addition, Patent No. 10-0839648 "hair loss prevention composition through reinforcement and moisturizing the dermal layer of the hair root" to reinforce the dermal layer of the hair root through the snail extract and bamboo sap composition, to improve the hair loss symptoms by moisturizing. In No. 10-2010-0097560, "Composition for the prevention of hair loss and hair growth promoting function using natural extracts, hair and scalp condition improvement" composition containing medicinal herbs, ginseng, henna extract, etc. to improve the anti-oxidation, anti-inflammatory, antibacterial, cell growth It has been found that it can be used as a composition for preventing hair loss, promoting hair growth, preventing dandruff, and alleviating inflammation of the scalp, and in the "composition for improving scalp and preventing hair loss" of Patent Publication No. 10-2014-0062249. Antioxidant activity, Anti-inflammatory activity and 5 extracts of chitoceros gracilis, a microalgae used in sea cucumber culture By identifying alpha-reductase activity inhibitory and developing hair loss prevention and improvement composition.

In addition, in the "patent for hair loss prevention or hair growth comprising a chrysanthemum extract" of Patent Publication No. 10-2014-0091376, experiments proved that chrysanthemum extract helps the activation of dermal papilla cells (hair loss) In the anti-hair loss prevention or hair growth composition comprising a peanut peel extract of Korean Patent Publication No. 10-2014-0092093, Peanut extract helps the growth promoting activation of dermal papilla cells and human keratinocytes. The anti-hair loss and promotion composition was developed through the experiment to give hair loss, and in the "Shampoo composition for improving the scalp and hair" of Korean Patent Publication No. 10-2014-0139930, traditional herbal ingredients are added to suppress the excessive production of sebum, antibacterial by dandruff, Prevents hair loss through the effects of improving hair and scalp troubles, such as moisturizing, adsorption, and increased flexibility due to far-infrared radiation and prevention of cracking Would.

In addition, in the "patent-free hair loss or wool-containing composition containing the extract of the herbal mixture" of Korean Patent Publication No. 10-2016-0023149, the growth of the dermal papilla cells and the like are extracted using extract compositions such as fish leaf, green leaf, green tea, and nasturtium. Proven in Korean Patent Publication No. 10-2017-0130213 "A composition for preventing hair loss and promoting hair growth using herbal medicines and a method for preparing the same", the first mixture using burdock, sewage, estuary, etc., perforated, donkey, mint, sessin, The second mixture containing licorice and the like is disclosed for a hair loss prevention and hair growth promoting composition comprising a ratio of 6: 4 to 6: 6, and the "hair loss prevention using the nipa palm flower" of Patent No. 10-1820531 In "Promoting hair growth composition" was developed a hair loss improvement and hair growth promoting composition through a mixture containing nipa palm flower extract and lotus root extract.

However, despite the research and efforts to overcome alopecia, the cause of hair loss is becoming more diverse, and the ever-increasing number of patients with hair loss is increasing, with the appearance of unprecedented social environmental problems such as scalp contamination caused by fine dust. . Therefore, there is a need for a new composition that can prevent and alleviate hair loss symptoms, including more diverse causes and problems of hair loss, including environmental problems.

Therefore, the present invention is to provide a composition for hair removal and hair growth using natural salts effective in hair loss relaxation, prevention and hair growth promotion by combining the natural salt with spinach extract, hwangchil extract and kelp extract.

The present invention for achieving the above object is characterized by making a composition having a composition ratio that can be effective in reducing and preventing hair loss, promoting hair growth by blending the natural salt with spinach extract, hwangchil extract, kelp extract, etc. do. In other words, the composition for hair loss alleviation and hair growth using the sun salt of the present invention comprises spinach extract, hwangchil extract and kelp extract in sun salt in a ratio of 1 to 5: 1 to 10: 0.1 to 0.5: 0.1 to 1 by weight. It is done.

The composition for alleviating hair loss and promoting hair growth using the sun salt according to the present invention efficiently removes contaminants and waste matters of the scalp and hair by osmotic pressure of salt, and promotes blood circulation, anti-inflammatory, anti-inflammatory, anti-inflammatory, and skin moisturizing effects of salt. By relieving inflammation of the scalp and helping soften keratin, hair loss can be alleviated and prevented.

In addition, the present invention is to effectively absorb the nutrients lacking in the scalp and hair through the composition such as spinach extract, kelp extract, hwangchil extract, thereby promoting hair growth and healthy scalp growth, to reduce the progress of hair loss, It can promote the growth of wool or hair growth, and has a useful effect as a composition for preventing hair loss or promoting hair growth.

1 is a graph of cytotoxicity results of dermal papilla cells (HFDPC-α) according to the sun salt content.
Figure 2 is a graph of cytotoxicity results of dermal papilla cells (HFDPC-α) according to the spinach extract content.
Figure 3 is a graph of cytotoxicity results of dermal papilla cells (HFDPC-α) according to the content of the yellow lacquer extract.
Figure 4 is a graph of cytotoxicity results of dermal papilla cells (HFDPC-α) according to the kelp extract content.
5 is a graph of cytotoxicity results for dermal papilla cells (HFDPC-a) according to the blending ratio.
6 is a graph of the results of proliferation of dermal papilla cells (HFDPC-α) according to sun salt content.
7 is a graph of the results of diffusion of dermal papilla cells (HFDPC-α) according to the spinach extract content.
Figure 8 is a graph of the results of diffusion of dermal papilla cells (HFDPC-α) according to the content of the yellow lacquer extract.
Figure 9 is a graph of the results of diffusion of dermal papilla cells (HFDPC-α) according to the content of kelp extract.
10 to 14 are graphs of the results of measurement of diffusion change of dermal papilla cells (HFDPC-α) according to the blending ratio.

Hereinafter, with reference to the accompanying drawings, preferred embodiments of the composition for hair loss alleviation and hair growth using the natural salt according to the present invention will be described in detail.

Depilation and hair growth promoting composition using sun salt according to an embodiment of the present invention, domestic salt, spinach extract, hwangchil extract, kelp extract as an active ingredient, estimate the optimal ratio between these four extracts to reduce hair loss and It provides a composition having the effect of promoting hair growth.

The salt has been used for beauty products such as skin care since ancient times. Egyptian cleopatra and Chinese poppies are said to have used salt when bathing, and in ancient Rome it was said that salt was used for beauty art. It is used for a purpose.

The osmotic action of salt effectively removes wastes in the body, promoting metabolism and helping blood circulation to swell well. In addition, salt helps balance body fluids, sterilization and anti-inflammatory effects help to relieve acne and rashes, and moisturize the skin and warm the body.

The herbaceous hardwood says, "Salt is cold and nonpoisonous. It kills poisons, sterilizes, strengthens the skin, heals the skin, prevents decay, eliminates odors, fleshes all kinds of wounds, Protects, detoxifies, chills, and dries dry. " And said. In addition, Heo-jun said in his agreement, "Salt is salty and has no poison. It controls poison, and heals hatred, heartache, and severe swelling of the lower part."

As you can guess from the above, salt is a natural product that can easily remove the wastes of pores without any side effects on the skin to keep the skin clean, and also as a natural beauty agent with excellent skin protection effect by strong sterilization and anti-inflammatory action. There is an attribute. Therefore, if it is developed as a material for scalp health and hair loss by using the salt as a cosmetic agent and the inherent weakness of salt, the effect is expected to be doubled compared to conventional hair loss materials.

On the other hand, salt is classified into natural salt, rock salt, mechanical salt, re-dealt salt, etc. according to the manufacturing method or production state. The natural salt is salt produced using sea water and the sun, and the salt salt is salt salted from mines. Mechanical salts (refined salts) are salts prepared by placing seawater in a filtration tank and immersing the water obtained from the ion exchange membrane in an evaporation tube. In addition, there are lake salts produced in lakes, and salt salts produced by using well water, and salt salts made by boiling sea water. In addition, depending on the processing method may be divided into grilled salt, bamboo salt, ocher salt.

In particular, the domestic natural salt is rich in natural minerals, the redox potential (ORP) of the domestic natural salt is less than 100 is the only salt of each country has a negative value. These ORPs appear low in good water, such as natural rock, and play a role in removing harmful oxygen from our bodies.

While the pH of imported salts is about 4-6, the natural salt of Korea's natural salt is almost alkaline, which is the most suitable salt for our body. Therefore, sun salt can be more reliably and variously effective in reducing hair loss and promoting hair growth than when using salts such as imported salt, refined salt and rock salt.

The spinach is a year-old or biennial grass belonging to the family of Ming Dynasty, which is cold-resistant and spread all over the world. According to the Journal of the American Medical Association, published in 1927, spinach has been used to treat anemia, indigestion, weakness, loss of energy, heart failure and kidney failure. do. Spinach is one of the foods high in carotenoids and helps prevent lung cancer. In addition, spinach has an active ingredient in the stomach and intestine, that is, it has a pharmacological action to purify the stomach, so it is effective in gastrointestinal disorders, constipation, coldness, and rough skin.

Spinach contains vitamins A, B1, B2, C, iron, calcium, carotene, and folic acid, as well as nutrients such as beta-carotene, lutein, phenol, dietary fiber, copper, manganese, and protein. have. In addition, spinach is rich in folate and iron, a type of vitamin B that helps to produce hair and alleviate hair loss, and also contains biotin, an essential ingredient for enhancing hair strength and tissue formation, to help with hair loss and promote hair growth. .

The Hwangchil Tree belongs to the family Arboraceae, and is native to Korea in the coastal area of Jeonnam and Jeju Island. It is an evergreen broad-leaved arboreous tree, divided into 3 ~ 5 leaves in the shape or oval, and looks like a flippers. The liquid flows out, called Hwangchil (黄 漆), and has been used for a long time as a paint for households, and recent studies have shown that there are antioxidants, boosts immunity, neurostable, antibacterial and anticancer.

The international scientific name of Hwangchil-tree is Dendro Panax Morbifera, which means "Panaxia", and contains saponin as much as ginseng tree. There is a record that it was 'Hwangchil Tree'.

The kelp is one of the abbreviated mobilization items prescribed by the Ministry of Health, China, rich in active iodine, and is already widely used for diseases caused by local iodine deficiency.

Kelp is rich in protein, vitamins, iodine, iron, calcium, sulfur, and zinc. In addition to carotene, xanthophylls, chlorophyll, and other pigments, carbohydrates such as carbonitization are made of carbohydrates such as nitrite and laminarin, and alginic acid, which is a component of cell walls, contains amino acids such as vitamin B2 and glutamic acid. have. Through pharmacological studies, kelp is known to have effects such as iodine supplementation, lowering blood pressure, anticoagulant, blood fat control and antitumor.

The following describes the process of making the composition of the present invention by finding the optimal composition ratio that is effective in alleviating hair loss and promoting hair growth by containing the extract as described above.

1. Preparation of Extract

The salt of the sea salt was used to remove the salt of water 1 to 3% by weight or less, and the raw salt was used as it is without processing the salt to remove the foreign matter.

The spinach, hwangchil (using yellow chile), kelp was purchased after the original, washed with water and used naturally dried for 3-5 days. The spinach was recovered 2g lyophilized when extracted dry 10g (500ml DW), the hwangchil was recovered 170g freeze-dried when extracted 1kg (10L DW) dry, the kelp is extracted 100g (1,000ml DW) dry 10 g lyophilisate was recovered. That is, spinach, hwangchil, kelp dried product: water = 1:10 After 100 ℃, pressurized hot water extraction for 8 hours, the extract was frozen in an ultra-cold freezer (-70 ℃) for 24 hours, and the frozen extract in a freeze dryer- Freeze-dried for 72 hours with temperature change from 45 ℃ to +50 ℃.

Usually, natural products (extracts) are extracted for 4 to 24 hours. By the way, the extraction raw material of this embodiment using a warm extraction method at 100 ℃, and further proceeds in the pressurized state, even if only 8 hours to extract the sufficient raw material extraction is possible.

Then, spinach, hwangchil, kelp was dissolved in 1g lyophilized in 10ml sterile water to prepare a final 0.1mg / ml stock (stock), in the case of sun salt sterilized sun salt and then dissolved 1g in 10ml sterile water to the final 0.1mg A stock of / ml was prepared. That is, all the raw materials were made to a concentration of 0.1g / ml and stored at -20 ℃. All experiments were then used after diluting the stock thus prepared. Here, the stock is a freeze-dried product, for example, when 10 ml of water is dissolved in 1 g, the concentration becomes 1 g / 10 ml, that is, 0.1 g / ml, which is referred to as stock.

On the other hand, all cell experiments were diluted with medium to grow cells after the preparation of high concentration stock. The reason for dilution with the medium is that the same condition medium should be added to the tested 96 well plate. That is, the stock was dissolved in sterile water, the cell culture medium was used when diluting each concentration in the cell experiment.

2. Experimental Example 1: Cytotoxicity test on dermal papilla cells (HFDPC-α)

After dispensing dermal papilla cells (HFDPC-α) into a 96 well plate at 1x10 ⁶ cell / well, sun salt (BSWE 0.1-5 mg / ml), spinach extract (SWE 0.1-5 mg / ml), and yellow lacquer extract (DPWE 0.01-0.5 mg). / ml), kelp extract (KWE 0.1 ~ 5mg / ml) was treated for each concentration and after 12 hours incubation was measured for absorbance at 450nm OD (Outer Diameter) and confirmed cytotoxicity. 1 to 4 are graphs of cytotoxicity results of dermal papilla cells (HFDPC-α) according to sun salt, spinach extract, yellow lacquer extract and kelp extract content.

1 to 4 show the cell survival rate by concentration, the cell survival rate is based on a reference value of 100 or more means no toxicity at all, less than that means some degree of toxicity. As a result of the experiment, as shown in Figures 1 to 4 almost no toxicity to the concentration of the sun salt 5mg / ml, showed little toxicity to the concentration of spinach extract 5mg / ml, toxicity to a concentration of 0.5mg / ml of yellow lacquer extract It did not show a significant toxicity up to 5mg / ml concentration of kelp extract.

3. Experimental Example 2: Cytotoxicity test on dermal papilla cells (HFDPC-a) according to the blending ratio

After dispensing dermal papilla cells (HFDPC-α) into a 96 well plate at 1x10 ⁶ cell / well, sun salt (BSWE 0.1-5 mg / ml), spinach extract (SWE 0.1-5 mg / ml), and yellow lacquer extract (DPWE 0.01-0.5 mg). / ml), kelp extract (KWE 0.1 ~ 5mg / ml) was treated according to each compounding ratio and after 24 hours of incubation, the cytotoxicity was confirmed after absorbance measurement at OD 450nm. Table 1 shows the compounding ratio of each sample number, Figure 5 is a graph of cytotoxicity results for dermal papilla cells (HFDPC-a) according to the compounding ratio.

Sample number Compounding ratio One standard 2 Natural Salt 2: Spinach 5: Yellow Chil 0.5: Kelp 1 3 Natural Salt 2: Spinach 5: Yellow Chil 0.3: Kelp 1 4 Natural Salt 2: Spinach 5: Yellow Chil 0.1: Kelp 1 5 Natural Salt 2: Spinach 3: Yellow Chil 0.5: Kelp 1 6 Natural Salt 2: Spinach 3: Yellow Chil 0.3: Kelp 1 7 Natural Salt 2: Spinach 3: Wild Chil 0.1: Kelp 1 8 Natural Salt 2: Spinach 1: Yellow Chil 0.5: Kelp 1 9 Natural Salt 2: Spinach 1: Yellow Chil 0.3: Kelp 1 10 Natural Salt 2: Spinach 1: Wild Chil 0.1: Kelp 1 11 Natural Salt 1: Spinach 5: Yellow Chil 0.5: Kelp 1 12 Natural Salt 1: Spinach 5: Yellow Chil 0.3: Kelp 1 13 Natural Salt 1: Spinach 5: Yellow Chil 0.1: Kelp 1 14 Natural Salt 1: Spinach 3: Yellow Chil 0.5: Kelp 1 15 Natural Salt 1: Spinach 3: Yellow Chil 0.3: Kelp 1 16 Natural Salt 1: Spinach 3: Yellow Chil 0.1: Kelp 1 17 Natural Salt 1: Spinach 1: Yellow Chil 0.5: Kelp 1 18 Natural Salt 1: Spinach 1: Yellow Chil 0.3: Kelp 1 19 Natural Salt 1: Spinach 1: Wild Chil 0.1: Kelp 1

As a result of the experiment, as shown in Figure 5, the blending ratio showing a survival rate of 70% or more were Samples 2, 6, 7, 8, 12-19.

4. Experimental Example 3: Diffusion experiment of dermal papilla cells (HFDPC-α)

After dispensing dermal papilla cells (HFDPC-α) into a 96 well plate at 1x10 ⁶ cell / well, sun salt (BSWE 0.1-5 mg / ml), spinach extract (SWE 0.1-5 mg / ml), and yellow lacquer extract (DPWE0.01-0.5) mg / ml) and kelp extracts (KWE 0.1 ~ 5mg / ml) were treated by concentration within the range of no cytotoxicity, and after 24 hours of incubation, the absorbance was measured at 490 nm and then proliferation was confirmed. Here, proliferation means the degree of proliferation and activation of cells. 6 to 9 are graphs showing the results of diffusion of dermal papilla cells (HFDPC-α) according to the contents of sun salt, spinach extract, yellow lacquer extract, and kelp extract.

As a result of the experiment, as shown in Figures 6 to 9, the sun salt did not show a change to 2mg / ml concentration, the diffusion was reduced at 5mg / ml. The diffusion of spinach extract increased as the concentration increased to 0.1 ~ 5mg / ml, and the diffusion increased as the concentration increased to 0.01 ~ 0.5mg / ml, and the diffusion of kelp extract increased to 0.1 ~ 2mg / ml. It decreased again at 5 mg / ml.

5. Experimental Example 4: Measurement of diffusion change of the dermal papilla cells (HFDPC-α) according to the mixing ratio

After dispensing dermal papilla cells (HFDPC-α) into a 96 well plate at 1x10 ⁶ cell / well, sun salt (BSWE 0.1-5 mg / ml), spinach extract (SWE 0.1-5 mg / ml), and yellow lacquer extract (DPWE 0.01-0.5 mg). / ml), the kelp extract (KWE 0.1 ~ 5mg / ml) was treated according to the following each mixing ratio and then incubated for 24 hours, after measuring the absorbance at OD 490nm and confirmed the diffusion. Table 2 shows the diffusion (%) value according to the mixing ratio of each sample. 10 and 11 are graphs of the results of measurement of diffusion change of the dermal papilla cells (HFDPC-α) according to the blending ratio.

Sample number Compounding ratio Diffusion (%) 24h One standard 100 2 Natural Salt 2: Spinach 5: Yellow Chil 0.5: Kelp 1 129.86 3 Natural Salt 2: Spinach 5: Yellow Chil 0.3: Kelp 1 123.147 4 Natural Salt 2: Spinach 5: Yellow Chil 0.1: Kelp 1 128.881 5 Natural Salt 2: Spinach 3: Yellow Chil 0.5: Kelp 1 95.035 6 Natural Salt 2: Spinach 3: Yellow Chil 0.3: Kelp 1 105.082 7 Natural Salt 2: Spinach 3: Wild Chil 0.1: Kelp 1 104.033 8 Natural Salt 2: Spinach 1: Yellow Chil 0.5: Kelp 1 83.543 9 Natural Salt 2: Spinach 1: Yellow Chil 0.3: Kelp 1 78.671 10 Natural Salt 2: Spinach 1: Wild Chil 0.1: Kelp 1 79.184 11 Natural Salt 1: Spinach 5: Yellow Chil 0.5: Kelp 1 119.953 12 Natural Salt 1: Spinach 5: Yellow Chil 0.3: Kelp 1 130.536 13 Natural Salt 1: Spinach 5: Yellow Chil 0.1: Kelp 1 122.634 14 Natural Salt 1: Spinach 3: Yellow Chil 0.5: Kelp 1 111.632 15 Natural Salt 1: Spinach 3: Yellow Chil 0.3: Kelp 1 107.716 16 Natural Salt 1: Spinach 3: Yellow Chil 0.1: Kelp 1 99.72 17 Natural Salt 1: Spinach 1: Yellow Chil 0.5: Kelp 1 96.9 18 Natural Salt 1: Spinach 1: Yellow Chil 0.3: Kelp 1 91.632 19 Natural Salt 1: Spinach 1: Wild Chil 0.1: Kelp 1 98.135

As a result of the experiment, the ideal diffusion value was shown at the compounding ratio of Samples 2 to 4, 12 and 13. That is, the diffusion value is 120 or more, and the cell growth and activation also increase at such a compounding ratio. Therefore, it was confirmed that the composition having the blending ratio of Samples 2 to 4, 12, 13 was effective in preventing hair loss and promoting hair growth.

6. Experimental Example 5: Measurement change of diffusion of dermal papilla cells (HFDPC-α) according to the mixing ratio

The experiment was performed in the same manner as in Experimental Example 4, but was composed of different mixing ratios from Experimental Example 4. Table 3 shows the diffusion values according to the mixing ratio of each sample. 12 is a graph showing the results of measurement of diffusion of dermal papilla cells (HFDPC-α) according to the blending ratio.

Sample number Compounding ratio Diffusion (%) 24h One standard 100 2 Natural Salt 3: Spinach 1: Wild Chil 0.1: Kelp 1 91.4 3 Natural Salt 3: Spinach 3: Yellow Chil 0.1: Kelp 1 92 4 Natural Salt 3: Spinach 5: Yellow Chil 0.1: Kelp 1 123.3 5 Natural Salt 3: Spinach 7: Yellow Chil 0.1: Kelp 1 110 6 Natural Salt 3: Spinach 10: Yellow Chil 0.1: Kelp 1 91 7 Natural Salt 4: Spinach 1: Yellow Chil 0.1: Kelp 1 105.9 8 Natural Salt 4: Spinach 3: Yellow Chil 0.1: Kelp 1 88.8 9 Natural Salt 4: Spinach 6: Wild Chil 0.1: Kelp 1 90.2 10 Natural Salt 4: Spinach 7: Yellow Chil 0.1: Kelp 1 91.1 11 Natural Salt 4: Spinach 10: Yellow Chil 0.1: Kelp 1 117.7 12 Natural Salt 5: Spinach 1: Yellow Chil 0.1: Kelp 1 126 13 Natural Salt 5: Spinach 3: Yellow Chil 0.1: Kelp 1 126.8 14 Natural Salt 5: Spinach 6: Wild Chil 0.1: Kelp 1 129.6 15 Natural Salt 5: Spinach 7: Yellow Chil 0.1: Kelp 1 123.3 16 Natural Salt 5: Spinach 10: Yellow Chil 0.1: Kelp 1 121.8 17 Natural Salt 3: Spinach 5: Yellow Chil 0.1: Kelp 0.1 73.8 18 Natural Salt 3: Spinach 5: Yellow Chil 0.1: Kelp 0.5 93.2 19 Natural Salt 3: Spinach 6: Wild Chil 0.1: Kelp 1 113.3 20 Natural Salt 4: Spinach 5: Yellow Chil 0.1: Kelp 0.1 127.4 21 Natural Salt 4: Spinach 5: Yellow Chil 0.1: Kelp 0.5 125 22 Natural Salt 4: Spinach 5: Yellow Chil 0.1: Kelp 1 123.3 23 Natural Salt 5: Spinach 5: Yellow Chil 0.1: Kelp 0.1 118 24 Natural Salt 5: Spinach 5: Yellow Chil 0.1: Kelp 0.5 105.5

As a result of the experiment, as shown in Figure 12, the sample 4, 12 to 16, showed an ideal diffusion value in the blending ratio of 20 to 22. That is, the diffusion value is 120 or more, and the cell growth and activation also increase at such a compounding ratio. Therefore, it was confirmed that the composition having a blending ratio of Samples 4, 12-16 and 20-22 was effective for preventing hair loss and promoting hair growth.

7. Experimental Example 6: Measurement of diffusion change of the dermal papilla cells (HFDPC-α) according to the mixing ratio

This experimental example is an experiment with cytotoxicity, unlike the above, after dispensing the dermal papilla cells (HFDPC-α) into a 96 well plate at 1x10 ⁶ cell / well, sun salt (BSWE 0.1-5 mg / ml), spinach extract ( SWE 0.1 ~ 5mg / ml), Hwangchil extract (DPWE 0.01 ~ 0.5mg / ml), kelp extract (KWE 0.1 ~ 5mg / ml) were treated according to each blending ratio below, followed by 24, 48 hours incubation, OD 490nm Absorbance was measured at, and then diffusion was confirmed. Table 4 is a diffusion value according to the mixing ratio of each sample, Figure 13 and Figure 14 is a graph of the results of the measurement of diffusion change of the dermal papilla cells (HFDPC-α) according to the mixing ratio.

Sample
number Compounding ratio cell
toxicity Diffusion (%) 24h Diffusion (%) 48h One standard 100 100 100 2 Natural Salt 2: Spinach 5: Yellow Chil 0.5: Kelp 1 71.88 129.86 115.99 3 Natural Salt 2: Spinach 5: Yellow Chil 0.3: Kelp 1 61.04 123.147 110.46 4 Natural Salt 2: Spinach 5: Yellow Chil 0.1: Kelp 1 63.19 128.881 113.43 5 Natural Salt 2: Spinach 3: Yellow Chil 0.5: Kelp 1 61.02 95.035 95 6 Natural Salt 2: Spinach 3: Yellow Chil 0.3: Kelp 1 70.53 105.082 102.16 7 Natural Salt 2: Spinach 3: Wild Chil 0.1: Kelp 1 73.28 104.033 95.04 8 Natural Salt 2: Spinach 1: Yellow Chil 0.5: Kelp 1 87.72 83.543 83.07 9 Natural Salt 2: Spinach 1: Yellow Chil 0.3: Kelp 1 65.92 78.671 98.55 10 Natural Salt 2: Spinach 1: Wild Chil 0.1: Kelp 1 63.2 79.184 83.31 11 Natural Salt 1: Spinach 5: Yellow Chil 0.5: Kelp 1 67.67 119.953 113.62 12 Natural Salt 1: Spinach 5: Yellow Chil 0.3: Kelp 1 103.49 130.536 109.94 13 Natural Salt 1: Spinach 5: Yellow Chil 0.1: Kelp 1 103.2 122.634 123.72 14 Natural Salt 1: Spinach 3: Yellow Chil 0.5: Kelp 1 98.15 111.632 100.7 15 Natural Salt 1: Spinach 3: Yellow Chil 0.3: Kelp 1 90.64 107.716 102.55 16 Natural Salt 1: Spinach 3: Yellow Chil 0.1: Kelp 1 71.94 99.72 101.69 17 Natural Salt 1: Spinach 1: Yellow Chil 0.5: Kelp 1 87.91 96.9 92.84 18 Natural Salt 1: Spinach 1: Yellow Chil 0.3: Kelp 1 85.47 91.632 96.87 19 Natural Salt 1: Spinach 1: Wild Chil 0.1: Kelp 1 110.98 98.135 100.88

In the above experimental example, based on the survival rate of 70% or more in the cytotoxicity experiment according to the compounding ratio, it was confirmed the diffusion value of the dermal papilla cells. In other words, if the cytotoxicity is greater than 70%, since it does not affect the human body, it is confirmed the diffusion value of the dermal papilla cells based on this value.

As a result of the experiment, the mixing ratio of Sample 2 was shown to be excellent in preventing hair loss and hair regrowth, and in the mixing ratio of Sample 12, there was a difference in diffusion value between 24 hours and 48 hours, And the hair growth promoting effect is excellent, in the case of the mixing ratio of the sample 13 was confirmed that there is no difference in the diffusion value effect over time and there is no cytotoxicity. Therefore, it was confirmed that the hair loss alleviation and hair growth promoting effect was the most excellent in the mixing ratio of the sample 13.

8. Experimental Results

In summary, the composition containing natural salt, spinach extract, hwangchil extract, and kelp extract showed the best effect on hair loss alleviation and hair growth at the mixing ratio (weight ratio) as shown in Table 5 below.

turn Compounding ratio One Solar salt 1: Spinach extract 5: Hwangchil extract 0.1: Kelp extract 1 2 Solar salt 1: Spinach extract 5: Hwangchil extract 0.3: Kelp extract 1 3 Natural salt 2: Spinach extract 5: Yellow lacquer extract 0.5: Kelp extract 1 4 Natural Sea Salt 3: Spinach Extract 5: Hwangchil Extract 0.1: Kelp Extract 1 5 Natural salt 4: Spinach extract 5: Hwangchil extract 0.1: Kelp extract 0.1 ~ 1 6 Solar salt 5: spinach extract 1 ~ 10: Hwangchil extract 0.1: Kelp extract 1

In summary, the composition for hair loss alleviation and hair growth using the sun salt of the present invention comprises a spinach extract, a yellow lacquer extract and a kelp extract in a sun salt in a ratio of 1 to 5: 1 to 10: 0.1 to 0.5: 0.1 to 1 by weight. It is effective for alleviating hair loss and promoting hair growth. That is, in the case of sun salt, even if it contains a large amount, there is no problem in the hair loss-releasing effect composition ratio, but it may be difficult to stabilize the product in developing the actual hair loss shampoo products, it is preferable to have the above ratio. And, in the case of the hwangchil extract, although the antimicrobial effect, etc. have been proved as a result of the experiment, it may be preferred to have a ratio as described above, which is a safe minimum amount of composition since allergies may occur depending on the constitution. In addition, the spinach extract and the kelp extract do not affect cytotoxicity or stabilization of the formulation, but preferably have the same ratio as described above, which is ideal for hair loss relaxation and hair growth promotion.

In the above description of the technical details for the hair loss-relieving and hair growth-promoting composition using the sun salt of the present invention has been described with the accompanying drawings, which will exemplarily explain the best embodiment of the present invention. Therefore, the present invention is not limited to the above-described embodiments, and various modifications and variations can be made without departing from the spirit and scope of the present invention. Examples or modifications will also belong to the claims of this invention.

Claims (7)

delete The sun salt uses salt less than 1 to 3% by weight by removing the brine,
Spinach, hwangchil, kelp used to freeze-dried after freezing the extract produced by pressurized hot water extraction of each freeze-dried,
The sun salt, the spinach extract, the hwangchil extract and the kelp extract is a 1: 5: 0.1: 1 composition for hair loss alleviation and hair growth using a sun salt, characterized in that it is formulated in a weight ratio. The sun salt uses salt less than 1 to 3% by weight by removing the brine,
Spinach, hwangchil, kelp used to freeze-dried after freezing the extract produced by pressurized hot water extraction of each freeze-dried,
The sun salt, the spinach extract, the hwangchil extract and the kelp extract is a 1: 5: 0.3: 1 composition for hair loss alleviation and hair growth using a sun salt, characterized in that it is formulated in a weight ratio. The sun salt uses salt less than 1 to 3% by weight by removing the brine,
Spinach, hwangchil, kelp used to freeze-dried after freezing the extract produced by pressurized hot water extraction of each freeze-dried,
The sun salt, the spinach extract, the hwangchil extract and the kelp extract is a 2: 5: 0.5: 1 composition for hair loss alleviation and hair growth using a sun salt, characterized in that it is formulated in a weight ratio. The sun salt uses salt less than 1 to 3% by weight by removing the brine,
Spinach, hwangchil, kelp used to freeze-dried after freezing the extract produced by pressurized hot water extraction of each freeze-dried,
The sun salt, the spinach extract, the hwangchil extract and the kelp extract is a composition for hair loss relief and hair growth with sun salt, characterized in that the formulation in a weight ratio of 3: 5: 1: 0.1. The sun salt uses salt less than 1 to 3% by weight by removing the brine,
Spinach, hwangchil, kelp used to freeze-dried after freezing the extract produced by pressurized hot water extraction of each freeze-dried,
The sun salt, the spinach extract, the hwangchil extract and the kelp extract is 4: 5: 0.1: 0.1 ~ 1 composition for hair loss alleviation and hair growth using a sun salt, characterized in that it is formulated in a weight ratio. The sun salt uses salt less than 1 to 3% by weight by removing the brine,
Spinach, hwangchil, kelp used to freeze-dried after freezing the extract produced by pressurized hot water extraction of each freeze-dried,
The sun salt, the spinach extract, the hwangchil extract and the kelp extract is a 5: 1 ~ 10: 0.1: 1 composition for hair loss relief and hair growth using a sun salt, characterized in that it is formulated in a weight ratio.

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