CN118078674A - Liposome encapsulating blue copper peptide or composition thereof and use thereof in hair growth - Google Patents
Liposome encapsulating blue copper peptide or composition thereof and use thereof in hair growth Download PDFInfo
- Publication number
- CN118078674A CN118078674A CN202410498545.3A CN202410498545A CN118078674A CN 118078674 A CN118078674 A CN 118078674A CN 202410498545 A CN202410498545 A CN 202410498545A CN 118078674 A CN118078674 A CN 118078674A
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- China
- Prior art keywords
- blue copper
- copper peptide
- liposome
- encapsulating
- fatty acid
- Prior art date
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention discloses liposome for encapsulating blue copper peptide or a composition thereof and application thereof in hair growth, belongs to the technical field of liposome, and particularly relates to liposome for encapsulating blue copper peptide, which comprises the following components: a core material comprising a blue copper peptide; an encapsulation material encapsulating the core material; the encapsulating material includes a polyglycerin fatty acid ester. Liposomes encapsulating the blue copper peptide and/or the biota orientalis extract are prepared by encapsulating a core material including the blue copper peptide and/or the biota orientalis extract, including an encapsulating material including a polyglycerin fatty acid ester. The liposome encapsulating the blue copper peptide and/or the biota orientalis extract prepared by the invention has the effects of improving the expression of promoting hair growth factors, having good cell proliferation effect, good testosterone reduction efficiency, good oil control effect, good skin permeability, thickening hair diameter, increasing growing period hair, reducing resting period hair, preventing alopecia and the like.
Description
Technical Field
The invention belongs to the technical field of liposome, and particularly relates to liposome for encapsulating blue copper peptide or a composition thereof and application of the liposome in hair growth.
Background
With age, the prevalence of alopecia increases, and the quality of life of the patient is significantly affected. The most common general categories of hair loss include androgenic alopecia (AGA) and seborrheic alopecia. Effective treatment for AGA is a significant direction. Unfortunately, few specific drugs capable of promoting hair growth for patients suffering from alopecia are available, and many methods are limited to inhibiting the phenomenon of alopecia, i.e., preventing alopecia, and the trouble of patients suffering from alopecia or hair rarefaction still exists.
The etiology of hair loss is multifactorial. Hormonal imbalance, genetic susceptibility, insufficient scalp blood flow, and loss of extracellular matrix proteins in the follicular bed are considered to be major contributors to hair loss. Hair follicles are important units of hair proliferation and differentiation, a dynamic micro-organ, consisting of different cell populations associated with ectodermal, mesodermal and neural crest origins. Hair follicles rely on specialized dermal and stromal cells located at the base for circulation and regeneration. In addition, several cytokines and growth factors, such as Vascular Endothelial Growth Factor (VEGF), basic fibroblast growth factor (bFGF), epidermal Growth Factor (EGF), insulin-like growth factor-1 (IGF-1) and transforming growth factor- β1 (TGF- β1), are known to be involved in regulating hair morphogenesis and in the growth of specialized dermal cells.
CN110151592a discloses a composition, a preparation method and application thereof, and enumerates a plurality of polypeptides with potential hair growth capability, and considers the stimulation recovery of the polypeptides to capillary vessels so as to achieve the effect of hair growth, but the key scene of omission in the process is as follows: conventional water-soluble polypeptides are difficult to penetrate into dermis layers, and have a nourishing effect on the hair follicle mouths blocked by grease, and also fail to provide good skin penetration promoting skills. In addition, in the prior invention, mainly for nourishing scalp to prevent alopecia, CN115590793A discloses a traditional Chinese medicine washing and caring liquid for improving sub-health states of scalp and hair, and a preparation method and application thereof, wherein the main emphasis is on nourishing effects of various Chinese herbal medicines mainly including biota orientalis on scalp so as to achieve the purposes of blackening hair, moisturizing and the like; CN112603844a discloses a jelly powder capable of rapidly generating blue copper peptide and a preparation method thereof, emphasis is placed on the preparation method of blue copper peptide, and the explanation of reasonable and efficient application of the jelly powder to hair loss prevention and hair growth is not clear.
Disclosure of Invention
The invention mainly emphasizes the scientific combination effect of the blue copper peptide and the biota orientalis extract, and the blue copper peptide wrapping technology is used for efficiently reaching the dermis layer to stimulate the regeneration of blood vessels and treating the alopecia problem of patients with hair follicle atrophy by virtue of the penetration promoting capability of the blue copper peptide wrapping technology. The combination of the two compositions can inhibit the high aggregation of propionibacterium acnes and malassezia on the scalp and solve the problem of blockage of follicular orifice due to excessive secretion of scalp grease while emphasizing mild hair growth.
The invention focuses on proving the effect of the invention on living body angiogenesis and supplying nutrition to hair follicles. Due to the hydrophilic characteristic, the conventional blue copper peptide stays on the surface of the scalp in the daily smearing process, is difficult to penetrate through the stratum corneum into the dermis layer to play a role, and in the penetration process, enzymes in the human body can degrade the blue copper peptide.
The action mechanism of the biota orientalis leaves is studied more, and the conclusion is different. The present invention has found that the individual components of the biota orientalis extract do not promote hair growth.
The invention aims to provide a liposome which encapsulates blue copper peptide or a composition thereof and has the performances of improving the expression of a hair growth promoting factor, having good cell proliferation effect, good testosterone reduction efficiency, good oil control effect, good skin permeability, thickening hair diameter, increasing hair in a growing period, reducing hair at a resting stage date of maturity, preventing hair loss and the like, and the application of the liposome in hair growth.
The technical scheme adopted by the invention for achieving the purpose is as follows:
a liposome encapsulating a blue copper peptide, comprising: a core material comprising a blue copper peptide; an encapsulation material encapsulating the core material; the encapsulating material comprises polyglycerol fatty acid ester, and the weight ratio of the blue copper peptide to the polyglycerol fatty acid ester is 1:1-300.
Preferably, the core material further comprises biota orientalis extract; alternatively, the encapsulating material further comprises a thickener.
More preferably, the weight part ratio of the biota orientalis extract to the blue copper peptide is 1:0.01-1000; or, the thickener comprises at least 1 of sclerotium gum, sodium hyaluronate and xanthan gum.
More preferably, the amount of thickener is 50-100wt% of the polyglycerin fatty acid ester.
The invention discloses a preparation method of liposome for encapsulating blue copper peptide, which comprises the following steps: preparing liposome for encapsulating blue copper peptide by adopting a thermal spraying mode from core material sample liquid and encapsulating material sample liquid; the core material sample liquid contains blue copper peptide, the encapsulating material sample liquid contains polyglycerol fatty acid ester, and the weight ratio of the blue copper peptide to the polyglycerol fatty acid ester is 1:1-300.
The invention discloses a composition containing blue copper peptide liposome, which comprises the liposome encapsulating blue copper peptide.
Preferably, the composition further comprises biota orientalis extract.
The invention discloses application of the liposome encapsulating the blue copper peptide in preparing a washing and caring product.
The invention discloses application of the composition containing the blue copper peptide liposome in preparing washing and caring products.
Preferably, in the preparation of the polyglycerin fatty acid ester solution, the polyglycerin fatty acid ester is added to ethanol and treated at 50-70 ℃ to prepare the polyglycerin fatty acid ester solution.
More preferably, the polyglycerin fatty acid ester solution contains 1 to 6wt% of polyglycerin fatty acid ester.
Preferably, in the preparation of the blue copper peptide sample liquid, blue copper peptide is added into water and is processed at 20-45 ℃ to prepare the blue copper peptide sample liquid.
More preferably, in the preparation of the blue copper peptide sample solution, the blue copper peptide sample solution contains 1-10wt% of blue copper peptide.
Preferably, in the preparation of the blue copper peptide liposome, the liposome is prepared in a thermal spraying mode, a polyglycerin fatty acid ester solution and a blue copper peptide sample solution are mixed at a nozzle of thermal spraying through a microfluidic system, micro-nano liquid drops are formed by thermal spraying under high pressure, polyglycerin fatty acid ester encapsulated particles are obtained after ethanol is volatilized completely, then the polyglycerin fatty acid ester encapsulated particles are added into water, after solidification, the mixture is recovered, air-dried to form polyglycerin fatty acid ester encapsulated microspheres, and then the polyglycerin fatty acid ester encapsulated microspheres are added into a thickening solution, and the mixture is freeze-dried to obtain the blue copper peptide liposome. After freeze-drying, the product may be shaped according to the envisaged shape to obtain liposomes of different shapes. The problem of skin delivery with conventional applications of blue copper peptide is always a daily chemical product pain spot, and palmitoyl tripeptide-1 disclosed in CN202210547095.3 has beneficial skin delivery properties, but its solubility in water is extremely low, and it can be dispersed in a glycerin system through certain operations, but too much glycerin introduction tends to provide a more suitable living environment for unbalanced flora of the scalp, further exacerbating the risk of seborrheic alopecia. The invention researches that the polyglycerol fatty acid ester can be used as an encapsulating material to encapsulate a core material comprising the blue copper peptide, so that the problem of skin delivery can be solved, meanwhile, a more suitable living environment is avoided being provided for unbalanced bacterial groups, the usage amount of the polyglycerol fatty acid ester has an important influence on skin delivery, and the better skin delivery effect can be achieved by regulating and controlling the relation between the encapsulating material and the amount of the core material, otherwise, the effect is poor or even ineffective, so that the invention discusses a proper lipid encapsulating technology and successfully completes the skin deep delivery of the blue copper peptide.
More preferably, in the thermal spraying treatment, the wind-heat environment in the heating sleeve is 30-50 ℃, and cooling water with the temperature of 0-10 ℃ is arranged at the bottom of the heating sleeve.
More preferably, the mixing amount of the polyglycerin fatty acid ester solution and the blue copper peptide-like liquid is 10-50 by volume ratio when mixing at the nozzle of the thermal spray: 1, the flow rate of the nozzle liquid is 0.1-0.3mL/s, and the nozzle air pressure is 2-5bar.
More preferably, the polyglycerol fatty acid ester encapsulated particles are added to water at 0-10 ℃.
More preferably, the thickening solution is formed by mixing a thickening agent and water, wherein the thickening solution contains 0.3-1wt% of the thickening agent, and the thickening agent comprises at least 1 of sclerotium gum, sodium hyaluronate and xanthan gum.
More preferably, the polyglyceryl fatty acid ester encapsulated microspheres, when added to the thickening solution, comprise from 50 to 100wt% of the polyglyceryl fatty acid ester based on the amount of polyglyceryl fatty acid ester therein.
Preferably, in the preparation of the biota orientalis extract, the biota orientalis raw material is added into ethanol solution, and is subjected to heating reflux extraction treatment for 2-5h at 70-90 ℃, and is filtered and separated, and the filtrate is concentrated and dried to remove residual water, so as to obtain the biota orientalis extract.
More preferably, the raw material of biota orientalis is 1 of biota orientalis, biota orientalis granules and biota orientalis powder after the crushing treatment or a mixture thereof.
More preferably, the ethanol solution contains 30-60wt% ethanol, and the amount of the biota raw material used is 5-30wt% of the ethanol solution.
More preferably, at least 1 of coarse filtration, adsorption filtration and fine filtration is used in the filtration separation, and the coarse filtration is to remove solid cake or granular biota orientalis residue after the extraction treatment; the adsorption filtration is combined filtration of active carbon and filter paper, the active carbon is positioned above the filter paper, and the cypress extracting solution is filtered and separated; the fine filtration is carried out by adopting a filter membrane with the pore diameter of 0.45 μm.
More preferably, the concentration is an evaporative concentration, either a conventional evaporation process or rotary evaporation, with the aim of removing the ethanol solvent. The purpose of the drying is to remove moisture.
Preferably, butyl butyrylacetate may also be added to the polyglyceryl fatty acid ester solution. The polyglycerin fatty acid ester solution contains 0.1-0.4wt% butyl butyrylacetate. The present invention has also found that butyl butyrylacetate can be used as an encapsulating material, and that when used together with a polyglyceryl fatty acid ester, butyl butyrylacetate has a better skin delivery effect.
Preferably, in the preparation of the arborvitae extract sample solution, the arborvitae extract is added into water and treated at 20-45 ℃ to prepare the arborvitae extract sample solution.
More preferably, the arborvitae extract sample solution contains 1-10wt% arborvitae extract.
Preferably, in the preparation of the biota extract liposome, the liposome is prepared by adopting a thermal spraying mode, a polyglycerol fatty acid ester solution and a biota extract sample solution are mixed at a nozzle of thermal spraying through a microfluidic system, thermal spraying is performed under high pressure to form micro-nano liquid drops, polyglycerol fatty acid ester encapsulated particles are obtained after ethanol is volatilized completely, then the polyglycerol fatty acid ester encapsulated particles are added into water, after solidification, the mixture is recovered and air-dried to form polyglycerol fatty acid ester encapsulated microspheres, and then the polyglycerol fatty acid ester encapsulated microspheres are added into a thickening solution, and freeze-drying is performed to obtain the biota extract liposome.
More preferably, the mixing amount of the polyglycerin fatty acid ester solution and the biota extract-like liquid at the nozzle of the thermal spray is 10-50 by volume: 1, the flow rate of the nozzle liquid is 0.1-0.3mL/s, and the nozzle air pressure is 2-5bar.
Preferably, in the preparation of the composition sample solution, the blue copper peptide sample solution and the biota extract sample solution are mixed to obtain the composition sample solution.
More preferably, the blue copper peptide sample solution and the biota extract sample solution have a mass ratio of 1: mixing at a ratio of 0.1-100.
Preferably, in the preparation of the composition liposome, the liposome is prepared by adopting a thermal spraying mode, a polyglycerin fatty acid ester solution and a composition sample solution are mixed at a nozzle of thermal spraying through a microfluidic system, micro-nano liquid drops are formed by thermal spraying under high pressure, polyglycerin fatty acid ester encapsulated particles are obtained after ethanol is completely volatilized, then the polyglycerin fatty acid ester encapsulated particles are added into water, after solidification, the mixture is recovered, air-dried to form polyglycerin fatty acid ester encapsulated microspheres, and then the polyglycerin fatty acid ester encapsulated microspheres are added into a thickening solution, and the mixture is freeze-dried to obtain the composition liposome.
More preferably, the mixing amount of the polyglycerin fatty acid ester solution and the composition sample solution is 10-50 by volume ratio when mixing at the nozzle of the thermal spray: 1, the flow rate of the nozzle liquid is 0.1-0.3mL/s, and the nozzle air pressure is 2-5bar.
Preferably, in the preparation of the complex, the blue copper peptide liposome and the biota extract sample liquid are mixed to obtain the complex.
More preferably, the blue copper peptide liposome and the biota orientalis extract sample liquid are mixed according to the mass ratio of 1: 10-200.
More preferably, a diluent may also be added to the compound to formulate the desired concentration. The diluent is water, ethanol or other reagents useful in the cleaning and care products.
The invention adopts the core materials including the blue copper peptide and/or the biota orientalis extract and the encapsulating materials including the polyglycerol fatty acid ester to prepare the liposome for encapsulating the blue copper peptide and/or the biota orientalis extract, thereby having the following beneficial effects: can improve the expression of promoting hair growth factor, has good cell proliferation effect, testosterone reduction efficiency, oil control effect, skin permeability, thickened hair diameter, increased growing period hair, reduced resting period hair, and alopecia prevention. Therefore, the liposome of the encapsulated blue copper peptide or the composition thereof has the advantages of improving the expression of the hair-promoting factors, along with good cell proliferation effect, good testosterone reduction efficiency, good oil control effect, good skin permeability, thickened hair diameter, increased hair in growing period, reduced hair resting date of maturity, hair loss prevention and the like, and the application of the liposome in hair growth.
Drawings
FIG. 1 is a graph showing the relative expression results of hair growth promoting factors.
FIG. 2 is a graph of absorbance after HaCaT cell proliferation.
FIG. 3 is a graph showing the effect of a arborvitae extract sample on cell proliferation.
FIG. 4 is a graph showing the effect of blue copper peptide-like liquid on cell proliferation.
FIG. 5 is a graph showing the effect of a composition sample on cell proliferation.
FIG. 6 is a graph showing the results of subcutaneous angiogenesis experiments.
FIG. 7 is a graph showing the signal of testosterone concentration after treatment of a arborvitae extract sample.
Fig. 8 is a graph showing testosterone concentration signal results after treatment with blue copper peptide-like fluid.
Fig. 9 is a graph showing the signal of testosterone concentration after treatment with a composition sample.
FIG. 10 is a graph showing the fluorescence intensity of cellular oils and fats after treatment with a biota extract sample.
FIG. 11 is a graph showing the results of fluorescence intensity of cellular oils and fats after treatment with a blue copper peptide sample solution.
FIG. 12 is a graph showing the fluorescence intensity of cell lipid after treatment with the composition sample.
FIG. 13 is a graph showing the amount of blue copper peptide in various skin layers.
FIG. 14 is a graph showing the content of blue copper peptide in dermis.
Fig. 15 is a hair diameter chart.
Fig. 16 is a plot of long term hair scale.
Fig. 17 is a diagram illustrating an example of a rest date of maturity.
Detailed Description
The technical scheme of the invention is further described in detail below with reference to the specific embodiments and the attached drawings:
Example 1: preparation method of liposome for encapsulating blue copper peptide
Preparation of polyglycerol fatty acid ester solution: adding polyglycerin fatty acid ester into ethanol, and processing at 60deg.C to obtain polyglycerin fatty acid ester solution. The polyglycerin fatty acid ester solution contains 3wt% of polyglycerin fatty acid ester.
Preparing blue copper peptide sample liquid: adding blue copper peptide into water, and processing at 40 ℃ to prepare blue copper peptide sample liquid. The blue copper peptide sample liquid contains 5wt% of blue copper peptide.
Preparation of blue copper peptide liposome: the liposome is prepared by adopting a thermal spraying mode, a polyglycerin fatty acid ester solution and a blue copper peptide sample solution are mixed at a nozzle of thermal spraying through a microfluidic system, thermal spraying is carried out under high pressure to form micro-nano liquid drops, polyglycerin fatty acid ester encapsulated particles are obtained after ethanol is completely volatilized, then the polyglycerin fatty acid ester encapsulated particles are added into water, after solidification, the polyglycerin fatty acid ester encapsulated particles are recovered and air-dried to form polyglycerin fatty acid ester encapsulated microspheres, and then the polyglycerin fatty acid ester encapsulated microspheres are added into a thickening solution, and freeze-drying is carried out to obtain the blue copper peptide liposome. In the thermal spraying treatment, the wind-heat environment in the heating sleeve is 40 ℃, and cooling water with the temperature of 10 ℃ is arranged at the bottom of the heating sleeve. When the mixture is carried out at the nozzle of the thermal spraying, the mixing amount of the polyglycerol fatty acid ester solution and the blue copper peptide sample solution is 30 by volume ratio: 1, the jet liquid flow rate was 0.2mL/s and the jet air pressure was 3bar. The polyglycerol fatty acid ester-encapsulated particles were added to water at 10 ℃. The thickening solution is formed by mixing a thickening agent and water, wherein the thickening solution contains 0.6wt% of the thickening agent, and the thickening agent is sclerotium rolfsii. When the polyglycerin fatty acid ester-encapsulated microspheres were added to the thickening solution, the amount of the thickener was 80% by weight of the polyglycerin fatty acid ester based on the amount of the polyglycerin fatty acid ester therein.
Example 2: preparation method of liposome for encapsulating blue copper peptide
The present example differs from example 1 only in that the mixing amount of the polyglycerin fatty acid ester solution and the blue copper peptide-like liquid is 20 by volume ratio: 1.
Example 3: preparation method of liposome for encapsulating blue copper peptide
This example differs from example 1 in the polyglycerin fatty acid ester solution.
Preparation of polyglycerol fatty acid ester solution: adding polyglycerin fatty acid ester and butyl butyrylacetate into ethanol, and processing at 60deg.C to obtain polyglycerin fatty acid ester solution. The polyglycerin fatty acid ester solution contains 3wt% of polyglycerin fatty acid ester and 0.15wt% of butyl butyrylacetate.
Example 4: preparation method of liposome for encapsulating blue copper peptide
This example differs from example 1 in the polyglycerin fatty acid ester solution.
Preparation of polyglycerol fatty acid ester solution: adding polyglycerin fatty acid ester and butyl butyrylacetate into ethanol, and processing at 60deg.C to obtain polyglycerin fatty acid ester solution. The polyglycerin fatty acid ester solution contains 3wt% of polyglycerin fatty acid ester and 0.3wt% of butyl butyrylacetate.
Example 5: preparation method of liposome for encapsulating biota orientalis extract
Preparation of biota orientalis extract: adding the raw material of the biota orientalis into ethanol solution, heating and refluxing at 80 ℃ for extraction for 3 hours, filtering and separating, concentrating filtrate, drying to remove residual water, and obtaining the biota orientalis extract. The raw material of the biota orientalis is biota orientalis after crushing treatment. The ethanol solution contains 50wt% of ethanol, and the consumption of the raw material of the biota orientalis is 20wt% of the ethanol solution. The filtering separation adopts coarse filtration, adsorption filtration and fine filtration treatment, wherein the coarse filtration is to remove solid block-shaped arborvitae residue after the extraction treatment; the adsorption filtration is combined filtration of active carbon and filter paper, the active carbon is positioned above the filter paper, and the cypress extracting solution is filtered and separated; the fine filtration is carried out by adopting a filter membrane with the pore diameter of 0.45 μm. The concentration is evaporation concentration, namely common evaporation treatment or rotary evaporation, and the purpose is to remove the ethanol solvent. The purpose of the drying is to remove moisture.
Preparing a biota orientalis extract sample liquid: adding the biota orientalis extract into water, and processing at 40deg.C to obtain biota orientalis extract sample solution. The arborvitae extract sample solution contains 5wt% arborvitae extract.
Preparation of biota orientalis extract liposome: the liposome is prepared by adopting a thermal spraying mode, a polyglycerin fatty acid ester solution and a biota extract sample solution are mixed at a nozzle of thermal spraying through a microfluidic system, thermal spraying is carried out under high pressure to form micro-nano liquid drops, polyglycerin fatty acid ester encapsulated particles are obtained after ethanol is completely volatilized, then the polyglycerin fatty acid ester encapsulated particles are added into water, after solidification, the polyglycerin fatty acid ester encapsulated particles are recovered and air-dried to form polyglycerin fatty acid ester encapsulated microspheres, and then the polyglycerin fatty acid ester encapsulated microspheres are added into a thickening solution, and freeze drying is carried out to obtain the biota extract liposome. In the thermal spraying treatment, the wind-heat environment in the heating sleeve is 40 ℃, and cooling water with the temperature of 10 ℃ is arranged at the bottom of the heating sleeve. When mixing at the nozzle of the thermal spraying, the mixing amount of the polyglycerol fatty acid ester solution and the biota extract sample solution is 30 by volume ratio: 1, the jet liquid flow rate was 0.2mL/s and the jet air pressure was 3bar. The polyglycerol fatty acid ester-encapsulated particles were added to water at 10 ℃. The thickening solution is formed by mixing a thickening agent and water, wherein the thickening solution contains 0.6wt% of the thickening agent, and the thickening agent is sclerotium rolfsii. When the polyglycerin fatty acid ester-encapsulated microspheres were added to the thickening solution, the amount of the thickener was 80% by weight of the polyglycerin fatty acid ester based on the amount of the polyglycerin fatty acid ester therein. The polyglycerin fatty acid ester solution was taken from example 1.
Example 6: preparation method of liposome for encapsulating biota orientalis extract
The present example differs from example 3 only in that the mixing amount of the polyglycerin fatty acid ester solution and the biota extract-like liquid is 20 in terms of volume ratio: 1.
Example 7: preparation method of composition liposome
Preparation of composition sample liquid: and mixing the blue copper peptide sample solution and the biota orientalis extract sample solution to obtain a composition sample solution. The mass ratio of the blue copper peptide sample liquid to the biota orientalis extract sample liquid is 1: 10. A blue copper peptide sample was obtained from example 1 and a biota extract sample was obtained from example 3.
Preparation of composition liposomes: the liposome is prepared by adopting a thermal spraying mode, a polyglycerin fatty acid ester solution and a composition sample solution are mixed at a nozzle of thermal spraying through a microfluidic system, thermal spraying is carried out under high pressure to form micro-nano liquid drops, polyglycerin fatty acid ester encapsulated particles are obtained after ethanol is volatilized completely, then the polyglycerin fatty acid ester encapsulated particles are added into water, after solidification, the polyglycerin fatty acid ester encapsulated particles are recovered and air-dried to obtain polyglycerin fatty acid ester encapsulated microspheres, and then the polyglycerin fatty acid ester encapsulated microspheres are added into a thickening solution, and freeze-drying is carried out to obtain the composition liposome. In the thermal spraying treatment, the wind-heat environment in the heating sleeve is 40 ℃, and cooling water with the temperature of 10 ℃ is arranged at the bottom of the heating sleeve. When mixing at the nozzle of the thermal spraying, the mixing amount of the polyglycerol fatty acid ester solution and the composition sample liquid is 30 in volume ratio: 1, the jet liquid flow rate was 0.2mL/s and the jet air pressure was 3bar. The polyglycerol fatty acid ester-encapsulated particles were added to water at 10 ℃. The thickening solution is formed by mixing a thickening agent and water, wherein the thickening solution contains 0.6wt% of the thickening agent, and the thickening agent is sclerotium rolfsii. When the polyglycerin fatty acid ester-encapsulated microspheres were added to the thickening solution, the amount of the thickener was 80% by weight of the polyglycerin fatty acid ester based on the amount of the polyglycerin fatty acid ester therein. The polyglycerin fatty acid ester solution was taken from example 1.
Example 8: preparation method of composition liposome
The present example differs from example 5 only in that the mixing amount of the polyglycerin fatty acid ester solution and the composition sample solution is 20 by volume ratio: 1.
Example 9: preparation method of compound
Preparation of the composite: and mixing the blue copper peptide liposome and the biota orientalis extract sample liquid to obtain the compound. Blue copper peptide liposome and biota orientalis extract sample liquid in a mass ratio of 1: 30. Blue copper peptide liposomes were taken from example 1 and biota orientalis extract-like liquid from example 3. Diluents may also be added to the compound to formulate the desired concentration. The diluent is water.
Example 10: preparation method of compound
Preparation of the composite: and mixing the blue copper peptide liposome and the biota orientalis extract sample liquid to obtain the compound. Blue copper peptide liposome and biota orientalis extract sample liquid in a mass ratio of 1: 80. Blue copper peptide liposomes were taken from example 1 and biota orientalis extract-like liquid from example 3. Diluents may also be added to the compound to formulate the desired concentration. The diluent is water.
Comparative example 1: preparation method of composition sample liquid
Preparation of composition sample liquid: and mixing the blue copper peptide sample solution and the biota orientalis extract sample solution to obtain a composition sample solution. The mass ratio of the blue copper peptide sample liquid to the biota orientalis extract sample liquid is 1: 1. A blue copper peptide sample was obtained from example 1 and a biota extract sample was obtained from example 3.
Comparative example 2: preparation method of liposome for encapsulating blue copper peptide
This comparative example is different from example 1 only in that in the preparation of the blue copper peptide liposome, the thickening solution in the preparation of the blue copper peptide liposome was not added with the sclerotium gum.
Comparative example 3: preparation method of liposome for encapsulating blue copper peptide
The comparative example differs from example 1 only in that in the preparation of the blue copper peptide liposome, the mixing amount of the polyglycerin fatty acid ester solution and the blue copper peptide-like liquid is 5 by volume ratio: 1.
Comparative example 4: preparation method of liposome for encapsulating blue copper peptide
The comparative example is different from example 1 only in that in the preparation of the blue copper peptide liposome, the mixing amount of the polyglycerin fatty acid ester solution and the blue copper peptide-like liquid is 60 by volume ratio: 1.
Comparative example 5: preparation method of liposome for encapsulating blue copper peptide
This comparative example is different from example 1 only in that the content of butyl butyrylacetate in the polyglyceryl fatty acid ester solution was 0.05wt%.
Comparative example 6: preparation method of liposome for encapsulating blue copper peptide
This comparative example is different from example 1 only in that the content of butyl butyrylacetate in the polyglyceryl fatty acid ester solution was 0.5wt%.
Test example:
1. Relative expression experiment of hair growth promoting factor
The experiment uses Quanti Gene Plex (QGP) method based on the Luminex 200 suspension chip multiplex detection system, combined with the detection of branched DNA technology, to directly measure RNA transcripts by signal amplification.
The study used papilla cells plated in 24 well plates at a density of 6X 10 4 cells per well, 0.5ml per well volume. Cell well plates were cultured in an incubator for 24h. The experiment was divided into three groups, each group containing 3 duplicate wells, control, first, second.
After the supernatant was aspirated by 24h culture, 0.2ml of a cell lysate (Lysis Mixture) was added to an orifice plate containing papilla cells to lyse the cells. The lysed cells were stored in an environment at-80 ℃.
In the experiment, the blue copper peptide sample liquid prepared in the example 1 and the biota orientalis extract sample liquid prepared in the example 3 are mixed and compounded, and water is used as a diluent to prepare the following test sample: in the first group, the biota orientalis extract in the test sample solution was 0.55wt% and the concentration of blue copper peptide was 60ppm; in the second group, the concentration of blue copper peptide was 30ppm at 0.25wt% of the biota extract in the test sample solution. In the experiment, the alopecia group human keratinocyte is used as a control group, and the blue copper peptide and the biota orientalis extract are not added into the control group. In this experiment, water was used as the diluent for the test sample. When the test sample is used according to the concentration, the blue copper peptide and the biota orientalis extract are respectively diluted to prepare a blue copper peptide sample liquid and a biota orientalis extract sample liquid, and then the blue copper peptide sample liquid and the biota orientalis extract sample liquid are added into DMEM for cell culture according to the concentration.
The experimental results are shown in figure 1, wherein on the basis of the treatment of the compound formula, the gene targets such as FGF, VEGF, KRT and the like affecting alopecia have obvious expression improvement; and the inflammatory factor IL6 is obviously inhibited, so that the aging of scalp and hair can be further slowed down.
2. Cell proliferation assay
(1) Cell proliferation assay of human keratinocytes (HaCaT)
The assay was performed using the cell counting kit CCK-8. Human keratinocytes (HaCaT) were seeded in 96-well plates at a density of 10 4 cells per well and incubated at 25 ℃ for 12h, 24h, 36h, at a concentration of 100, 200, 400ppm of blue copper peptide, with DMEM-treated cells as controls. The proliferation of blue copper peptide on HaCaT was measured with an enzyme-labeled instrument. In the experiment, water is used as a diluent to prepare the blue copper peptide sample liquid. When the blue copper peptide sample solution is used at the above concentration, the blue copper peptide sample solution is added to DMEM for cell culture in a compounding amount.
The test result is shown in figure 2, and the HaCaT treated by the blue copper peptide has obvious cell proliferation effect, and proves that the HaCaT has the effect of promoting hair growth. At the same concentration, the detection results at 12h, 24h and 36h are represented from left to right, respectively.
(2) Cell proliferation assay of papilla cells
The assay was performed using the cell counting kit CCK-8. Hair papilla cells were inoculated into 96-well plates at a density of 10 4 cells per well, cultured at 25℃for 12 hours, 24 hours, and 36 hours, and proliferation of hair papilla cells was measured with an ELISA reader.
In the experiment, water is used as a diluent to prepare a arborvitae extract sample liquid, a blue copper peptide sample liquid and a composition sample liquid respectively. When the biota extract sample liquid, the blue copper peptide sample liquid and the composition sample liquid are used according to specific concentrations, the biota extract sample liquid, the blue copper peptide sample liquid and the composition sample liquid are respectively added into DMEM used for cell culture according to the mixing amount, and the related concentrations are as follows:
Setting a blank control group, wherein blue copper peptide and biota orientalis extract are not added in the blank control group; setting a positive control group, adding bovine embryo serum into the positive control group, wherein the concentration is 10%, and adding the mixed amount into DMEM used for cell culture; setting a arborvitae extract sample liquid group to prepare the arborvitae extract sample liquid group with the concentration of 2.2, 1.1, 0.55 and 0.275 wt%; setting a blue copper peptide sample liquid group, and preparing 30, 60 and 120ppm; the composition sample liquid set was set up to prepare 0.1375wt% of biota orientalis extract-15 ppm of blue copper peptide set, 0.275wt% of biota orientalis extract-30 ppm of blue copper peptide set and 0.55wt% of biota orientalis extract-60 ppm of blue copper peptide set.
The test result of the arborvitae extract sample liquid is shown in figure 3, the test result of the blue copper peptide sample liquid is shown in figure 4, and the test result of the composition sample liquid is shown in figure 5; in FIG. 5, 2.5% represents 0.1375% by weight of the extract of Platycladus orientalis-15 ppm of the blue copper peptide group, 5% represents 0.275% by weight of the extract of Platycladus orientalis-30 ppm of the blue copper peptide group, and 10% represents 0.55% by weight of the extract of Platycladus orientalis-60 ppm of the blue copper peptide group; compared with a blank control group, the cell viability of the positive control group is obviously increased, which indicates that the experimental system is effective; compared with a blank control group, the biota orientalis and the blue copper peptide sample liquid have the capability of promoting cell proliferation under a certain concentration, and have repairing effect; the compound preparation contains 60ppm blue copper peptide and 0.55% concentration of biota orientalis extract, has the proliferation capability of single component under high concentration, and has the advantages of excellent product effect and obvious synergistic effect. The concentration in the compound preparation can be 600ppm blue copper peptide and 5.5 percent of the biota orientalis extract, so that the effect is better.
3. Subcutaneous angiogenesis experiments
A model of testosterone induced alopecia was established using C57BL/6 mice. The hair of the mice was shaved off with an electric razor, at which time the hair was in rest and the skin of the dehaired mice appeared pink. Mice were randomly divided into 2 groups: control and experimental groups. Testosterone solution (0.05% in 75% ethanol) was topically applied once daily to the shave areas of all mice backs except the control group, 250 μl each time. After 30 minutes of testosterone treatment, 1000 μl of blue copper peptide-like liquid was uniformly applied to the shaved back. After 30 days of application, mice were euthanized and photographed after H & E staining of the back dermal tissue. In the experiment, the concentration of the blue copper peptide sample solution is 600ppm, and the diluent is water.
The test results are shown in FIG. 6, and the dermis of the mice treated in the experimental group is seen as a new blood vessel with red blood cells inside, compared with the dermis of the mice treated in the control group, which is located at the position indicated by the arrow in the figure.
4. Alpha reductase protocol
The experimental model is type II 5α -reductase, which has been shown to induce hair loss by reducing testosterone levels in the body, an intrinsic factor. The experimental ingredients were added to a testosterone solution of 2.5mg/mL at the corresponding concentrations and mixed. Testosterone was reduced by the addition of 80U of 5α -reductase. After the reaction is completed, high Performance Liquid Chromatography (HPLC) is adopted to measure the change of the testosterone amount of the substrate in the enzymatic reaction, so as to reflect the inhibition degree of each experimental component on the enzyme activity.
In the experiment, water is used as a diluent to prepare a arborvitae extract sample liquid, a blue copper peptide sample liquid and a composition sample liquid respectively. The biota extract sample solution, the blue copper peptide sample solution and the composition sample solution are used according to specific concentrations, and the related concentrations are as follows:
Setting a blank control group, wherein blue copper peptide and biota orientalis extract are not added in the blank control group; setting a negative control group, adding finasteride into the negative control group, wherein the concentration is 1 mu mol/L, and the solvent is water; setting a arborvitae extract sample liquid group to prepare the arborvitae extract sample liquid group with the concentration of 22, 5.5 and 2.75 weight percent; setting a blue copper peptide sample liquid group, and preparing 6, 60 and 600ppm; the composition sample liquid group was set up, and 2.75wt% of the biota orientalis extract-300 ppm of the blue copper peptide group, 5.5wt% of the biota orientalis extract-600 ppm of the blue copper peptide group and 11wt% of the biota orientalis extract-1200 ppm of the blue copper peptide group were prepared.
The test result of the arborvitae extract sample liquid is shown in fig. 7, and sample 1 is arborvitae extract sample liquid; the test result of the blue copper peptide sample liquid is shown in fig. 8, and sample 2 is the blue copper peptide sample liquid; the test result of the composition sample liquid is shown in fig. 9, and a sample 3 is formed by mixing a sample 1 and a sample 2; wherein, the complete reaction is that the determined 80U active 5-alpha reductase reacts with the testosterone solution with the designated concentration, and part of testosterone is still detected after the complete reaction. Under the experimental conditions, the compound product has better testosterone reduction efficiency than finasteride, and has the anticreep effect.
5. Oil control experiment
Based on human immortalized sebaceous gland cell SZ95, the method uses hormone substance 5α DHT to stimulate, establishes an in vitro grease secretion model, and evaluates the oil control efficacy of the object to be tested through qualitative analysis after administration treatment. And determining the lipid drop quantity by detecting the fluorescence intensity, and detecting the lipid drop distribution condition before and after the action of the sample.
In the experiment, water is used as a diluent to prepare a arborvitae extract sample liquid, a blue copper peptide sample liquid and a composition sample liquid respectively. When the biota extract sample liquid, the blue copper peptide sample liquid and the composition sample liquid are used according to specific concentrations, the biota extract sample liquid, the blue copper peptide sample liquid and the composition sample liquid are respectively added into DMEM used for cell culture according to the mixing amount, and the related concentrations are as follows:
Setting a blank control group, wherein blue copper peptide and biota orientalis extract are not added in the blank control group; setting a positive control group, adding retinoic acid into the positive control group, wherein the concentration is 3 mug/mL, and adding the matched amount into DMEM used for cell culture; setting a arborvitae extract sample liquid group to prepare the arborvitae extract sample liquid group with the concentration of 2.2, 1.1, 0.55 and 0.275 wt%; setting a blue copper peptide sample liquid group, and preparing 30, 60 and 120ppm; the composition sample liquid set was set up to prepare 0.1375wt% of biota orientalis extract-15 ppm of blue copper peptide set, 0.275wt% of biota orientalis extract-30 ppm of blue copper peptide set and 0.55wt% of biota orientalis extract-60 ppm of blue copper peptide set.
The results of the test of the arborvitae extract sample liquid are shown in FIG. 10, the results of the test of the blue copper peptide sample liquid are shown in FIG. 11, and the results of the test of the composition sample liquid are shown in FIG. 12, wherein 2.5 represents 0.1375wt% arborvitae extract-15 ppm blue copper peptide group, 5 represents 0.275wt% arborvitae extract-30 ppm blue copper peptide group, and 10 represents 0.55wt% arborvitae extract-60 ppm blue copper peptide group; compared with the BC group, the NC group has obviously increased fluorescence intensity, which indicates that the induction stimulation is effective; the blue copper peptide and the biota orientalis extract have different degrees of oil control effects, and the compound formula still has the oil control effects at lower concentrations of 2.5%, 5% and 10%, so that the compound effect is proved to be good.
6. Skin penetration test
Intact hairless mouse skin was mounted in Franz diffusion cells with the epidermal side facing the donor chamber. The receptor chamber was filled with HEPES buffer (10 mM, pH 7.4) mixed with 15% ethanol containing PMSF and PNT as protease inhibitors (final concentrations of 5mM and 1mM, respectively). The donor chamber was charged with 1mlCu-GHK or liposome-microparticle encapsulated blue copper peptide (Cu-GHK@LP) solution. The skin was removed from the diffusion cell 24 hours after sample preparation. The donor solution remaining on the skin surface was removed by washing with 1ml of distilled water (repeated 4 times). The fluff-free absorbent wet wipes were used to dry the skin. The stratum corneum was carefully collected with a blunt spatula. The epidermis is separated from the dermis layer using a blunt knife. Each of the separated skin layers (stratum corneum, epidermis and dermis) was minced using scissors. Cu-GHK or Cu-GHK@LP24 hours distributed in each skin layer was extracted with 1ml of methanol with continuous shaking. After 24 hours of extraction, the samples were centrifuged and the supernatants were analyzed by LC-MS/MS, the experimental results being used to assess the amount of peptide remaining in the skin layers.
In the experiment, cu-GHK@LP is the blue copper peptide liposome prepared in the embodiment 1, cu-GHK is blue copper peptide, and the diluents of the Cu-GHK and the blue copper peptide liposome are water and are diluted to a specific concentration for use.
As shown in the test results in FIG. 13, the conventional blue copper peptide can stay in the stratum corneum part in the smearing process, is extremely difficult to permeate, has extremely low content in the epidermis part, and has no blue copper peptide in the dermis; the liposome encapsulated blue copper peptide is about 15 times as transdermally absorbable as conventional blue copper peptide and reaches up to the dermis.
The skin permeation experiments are carried out on the products prepared in each example and the comparative example, the content of the blue copper peptide in the dermis layer is measured, the test result is shown in fig. 14, in the technical scheme of the comparative example 1, the blue copper peptide cannot reach the dermis layer of the skin, after the core materials including the blue copper peptide are directly encapsulated by the polyglycerol fatty acid ester serving as an encapsulating material, a thickening agent serving as an encapsulating material is also needed, and the thickening agent not only can enable the liposome to be shaped easily, but also can form a composite structure with the polyglycerol fatty acid ester, and after the product is applied to the skin permeation experiment, the blue copper peptide can be absorbed under the skin better, and the amount reaching the dermis layer is increased; in the process of preparing the liposome, the content of the polyglycerol fatty acid ester and the blue copper peptide is found to be in a certain range, so that the liposome has better skin permeation effect, and the use amount of the polyglycerol fatty acid ester is too high or too low, so that the skin permeation effect of the blue copper peptide is poor; furthermore, the invention also discovers that after butyl butyrylacetate and polyglyceryl fatty acid ester are mixed together to prepare polyglyceryl fatty acid ester solution, the prepared liposome has better skin permeation effect, and experiments on the usage amount of butyl butyrylacetate show that the usage amount of butyl butyrylacetate cannot be too high or too low, otherwise, the usage amount of butyl butyrylacetate cannot be better to improve the skin permeation effect of the liposome.
7. Human body experiment
(1) Experiments were performed according to the method of the national food and drug administration, announcement about incorporating 7 methods of testing preservatives in cosmetics, etc. into the cosmetic safety Specification (2015 edition), no. 17 of 2021
The compound formulation used in this embodiment is: water is used as a diluent to prepare a compound sample liquid, and the compound sample liquid is compounded to 5.5wt% of biota orientalis extract-600 ppm of blue copper peptide group, wherein the blue copper peptide is the actual effective concentration in the blue copper peptide liposome.
In the experiment, the combination of the blue copper peptide liposome and the biota orientalis extract is adopted for experiment, and the measurement of mild and non-irritation, tightening, hair thickening diameter, hair increase in growing period, hair reduction in resting date of maturity and the like are carried out.
The experimental method comprises the following steps: cosmetic is evaluated by 30 healthy male or female subjects with thin and soft hair, excessive hair loss and mild and sparse hair, and more than 10 hair loss counts after 2 weeks of wash-out period according to a 60-time combing method using test samples, and by means of instrument detection, image analysis and clinical evaluation.
Evaluation index:
hair diameter (μm): the thickness of the hair in the region;
Growth phase/rest date of maturity hair ratio: from the dermatoscopic image after shaving hair for two days, the ratio of occurrence of the head for the long term and the rest period was calculated based on the principle that the hair in the rest period does not grow but the hair in the growing period grows.
Measurement area, shaving area: occipital bone, approximately 2cm diameter circular in size.
Measuring instrument: the analysis management system Photo Max PRO (DMS, austria).
The results of the hair diameter test are shown in fig. 15, and the test samples were continuously used for 4 weeks, and the hair diameter of the subject was not significantly changed (p > 0.05) from the basal value; the test samples were used continuously for 8 weeks, with no significant change in hair diameter of the subjects (p > 0.05) from the basal value; the test samples were used continuously for 12 weeks, and the hair diameter of the subjects had a significant increase (0.01 < p.ltoreq.0.05) from the basal value, with a rate of increase of 5.80%.
The test results of the anagen hair ratio are shown in FIG. 16, and the anagen hair ratio of the subject is remarkably increased (0.001 < p.ltoreq.0.01) compared with the basal value by 9.23% after continuously using the test sample for 4 weeks; the test sample is continuously used for 8 weeks, the hair proportion in the growing period of the subject is remarkably increased (0.001 < p is less than or equal to 0.01) compared with a basic value, and the rate of increase is 13.47%; the test sample was used continuously for 12 weeks, and the hair proportion in the anagen phase of the subject was significantly increased (0.01 < p.ltoreq.0.05) compared with the basal value, with an increase rate of 16.09%.
As shown in FIG. 17, the test sample was continuously used for 4 weeks, and the hair percentage at rest date of maturity was very significantly reduced (0.001 < p.ltoreq.0.01) compared with the basal value, the reduction rate was 8.72%; the test sample is continuously used for 8 weeks, the resting date of maturity rate of the test subject is obviously reduced (p is less than or equal to 0.01< 0.05) compared with the basic value, and the reduction rate is 12.09%; the test sample was used continuously for 12 weeks, and the subject's resting date of maturity rate was significantly reduced (0.01 < p.ltoreq.0.05) compared to the basal value, at a reduction of 15.20%.
In conclusion, the blue copper peptide and the biota orientalis extract test product are continuously used for 4 weeks, and the effects of increasing hair in the growing period, reducing hair at rest date of maturity, mildly and without stimulation and tightening are achieved; the blue copper peptide and the biota extract test product are continuously used for 8 weeks, and the hair growth period hair is increased and the hair rest period hair is reduced; the blue copper peptide and the biota extract test product are continuously used for 12 weeks, and have the effects of thickening hair diameter, increasing hair in growing period and reducing hair in resting period.
(2) Experiments are carried out according to the test method of the anti-hair loss efficacy of the cosmetic in chapter 6 of cosmetic safety technical Specification (2015 edition)
The compound formulation used in this embodiment is: water is used as a diluent to prepare a compound sample liquid, and the compound sample liquid is compounded to 5.5wt% of biota orientalis extract-600 ppm of blue copper peptide group, wherein the blue copper peptide is the actual effective concentration in the blue copper peptide liposome.
Hair loss count: subjects were combed with their hair by a trained staff member at each visit using a 60-pass combing method, the hair loss counted, and recorded.
Hair density assessment: local evaluation: fixing a hair cutting area (the top of the head is inclined to the temporal side) of at least 1.5cm multiplied by 1.5cm on the head of a subject, positioning, and ensuring that the hair cutting area is consistent when each visit is performed, wherein the hair is cut until the residual length is not more than 1mm; in the process of image acquisition, an operator needs to enable a subject to be in a comfortable position, a dermatoscope is placed at the center of a hair cutting area to carry out local hair image shooting, a dermatoscope lens is completely attached to scalp and kept vertical during shooting, and the definition of a shot image is checked. The number and density of local hairs are counted and recorded using image analysis software or manual counting methods.
Table 1 difference in hair loss count before and after use (mean.+ -. Standard deviation)
Table 2 product local hair Density before and after use (mean.+ -. Standard deviation)
According to the test result, the difference of the hair loss count at the 12 th week after the use of the product is obviously lower than that of a control group (p is less than 0.05), the local hair density at the 8 th week and the 12 th week after the use of the product is obviously increased (p is less than 0.05), and the product is considered to have the hair loss prevention effect.
The above examples and/or embodiments are merely for illustrating the preferred embodiments and/or implementations of the present technology, and are not intended to limit the embodiments and implementations of the present technology in any way, and any person skilled in the art should be able to make some changes or modifications to the embodiments and/or implementations without departing from the scope of the technical means disclosed in the present disclosure, and it should be considered that the embodiments and implementations are substantially the same as the present technology.
The principles and embodiments of the present application have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the method of the present application and its core ideas. The foregoing is merely illustrative of the preferred embodiments of this application, and it is noted that there is objectively no limit to the specific structure disclosed herein, since numerous modifications, adaptations and variations can be made by those skilled in the art without departing from the principles of the application, and the above-described features can be combined in any suitable manner; such modifications, variations and combinations, or the direct application of the inventive concepts and aspects to other applications without modification, are contemplated as falling within the scope of the present application.
Claims (9)
1. A liposome encapsulating a blue copper peptide, comprising: a core material comprising a blue copper peptide; an encapsulation material encapsulating the core material; the encapsulating material comprises polyglycerol fatty acid ester, and the weight ratio of the blue copper peptide to the polyglycerol fatty acid ester is 1:1-300.
2. A liposome encapsulating a blue copper peptide according to claim 1, wherein: the core material further comprises a biota orientalis extract; alternatively, the encapsulating material further comprises a thickener.
3. A liposome encapsulating a blue copper peptide according to claim 2, wherein: the weight part ratio of the biota orientalis extract to the blue copper peptide is 1:0.01-1000; or, the thickener comprises at least 1 of sclerotium gum, sodium hyaluronate and xanthan gum.
4. A liposome encapsulating a blue copper peptide according to claim 2, wherein: the thickener is 50-100wt% of polyglycerol fatty acid ester.
5. A method for preparing the blue copper peptide-encapsulated liposome according to any one of claims 1 to 4, comprising: preparing liposome for encapsulating blue copper peptide by adopting a thermal spraying mode from core material sample liquid and encapsulating material sample liquid; the core material sample liquid contains blue copper peptide, the encapsulating material sample liquid contains polyglycerol fatty acid ester, and the weight ratio of the blue copper peptide to the polyglycerol fatty acid ester is 1:1-300.
6. A composition comprising a blue copper peptide liposome comprising the blue copper peptide-encapsulated liposome of claim 1.
7. The composition comprising a blue copper peptide liposome according to claim 6, wherein: the composition further comprises an extract of biota orientalis.
8. Use of the liposome encapsulating a blue copper peptide according to any one of claims 1-4 for the preparation of a washing product.
9. Use of a composition comprising a blue copper peptide liposome according to any one of claims 6 to 7 in the manufacture of a laundry product.
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