KR20100031040A - Chinese composition for hair protection and hair growth-promotion and methods for preparing the same - Google Patents

Abstract

PURPOSE: A medicinal herb composition for preventing hair loss and promoting hair growth is provided to collect essential oil ingredient, aromatic composition and anti-oxidative ingredient during hydrothermal extraction. CONSTITUTION: A medicinal herb composition preventing hair loss and promoting hair growth contains extract of black rice, black soybean, black sesame, mulberry, grape, Acanthopanax spp. plum, Rubus coreanus Miquel, blueberry and black olive. A method for manufacturing the medicinal herb composition for preventing hair loss and promoting hair growth comprises: a step of heat-extracting black rice, black soybean, and black sesame; a step of mixing mulberry, grape, Acanthopanax spp. plum, Rubus coreanus Miquel, blueberry and black olive and heat-extracting; a step of cooling and collecting steam obtained during heat-extraction; and a step distilled liquid at a ratio of 1-3:3-1.

Description

Herbal composition for preventing hair loss and promoting hair growth and manufacturing method thereof {Chinese Composition for Hair Protection and Hair Growth-Promotion and Methods for Preparing the Same}

The present invention relates to a herbal composition for preventing hair loss and promoting hair growth, and a method of manufacturing the same.

Modern people are faced with excessive stress and air pollution, and hair loss is spreading regardless of age and gender due to skin irritation caused by hair products such as ultraviolet rays, sprays, and mousse.

Hair protects and protects the human body from external stimuli such as buffering and external stimuli such as ultraviolet rays, temperature, and friction, and absorbs heavy metals such as arsenic, mercury, and zinc that are unnecessary to the body and releases them to the body. It is an important aesthetic factor in the expression of external images. Hair is only a mammal, and as an appendage to the skin, hair starts from 9 to 12 weeks of birth and is inherited by parental inheritance. Hair grows in follicles. The hair is divided into three periods according to the hair cycle: anagen, catagen, and telogen. Hair grows only in the growth phase and each hair growth cycle is independent and has its own cycle. Therefore, the whole picture does not fall off at one time. Resting hair is about 70-120 a day, and naturally dropping out every day. Hair cycles are divided into 5-6 years of growth, 2 to 3 weeks of degeneration, and 2 to 3 months of rest. Hair growth is influenced by various internal and external factors, such as nutritional status of the individual, hormonal temperature consistent systemic and disease, and various chemicals. Especially, when the epidermal overgrowth of the scalp is caused by some cause, It is known. These days, the physical rhythm is broken due to various causes such as environmental, genetic, mental, and physical, and the hair follicles as well as the scalp are greatly affected. Because of this, hair damage is basic, and it becomes dry or sensitive, and it appears in various forms such as hair loss.

Accordingly, research has been conducted on compositions that can improve the condition of the scalp and hair by preventing abnormalities of various scalp and hair including hair loss. Such compositions should exhibit excellent hair loss prevention, hair growth, and wool effects. In addition, since the product of the composition used for the scalp and hair is frequently used for a long time, it should not show side effects such as irritation or damage. However, related products on the market are insufficient to show the expected improvement of the scalp and hair, and the main ingredient is often a chemical, which often causes side effects on the scalp. Therefore, there is a need for the development of a composition for preventing hair loss and promoting hair growth that is excellent in preventing hair loss and promoting hair growth and having no side effects even in long-term use.

On the other hand, in the Orient, foods with black color are regarded as' foods eaten by freshness' and 'foods that dominate life's life (神農 本草 經). In addition, according to the principle of five-point theory, the color of food is closely related to each function of our body. In other words, in five organs, the foods of blue in liver (intestine), red in heart (intestine), yellow in intestine (intestine), white in lung (intestine) and black in kidney (intestine) are beneficial. It was.

In one medicine, the kidney refers to the organs that maintain vitality, including the kidneys, the adrenal glands, the urinary bladder, and the genitals. In particular, Chinese medicine is known to be involved in hair. According to the five theory, it is the black food that presides over this god.

This important organ, which benefits God is black food. Black is a special color in nature that absorbs the sun and heats itself. Black foods with this property have a far-infrared effect that warms the body from the inside when it enters our bodies. In other words, when the stomach is warmed, gastric juice secretion is promoted, digestive activity is active, and when the intestine is warmed, the activity of the intestine is active, which is effective in relieving constipation and preventing obesity. In addition, the dark part of the black pigment is in a carbonized (炭化) state to absorb unnecessary substances, which absorbs waste products, fat, cholesterol in the large intestine and discharges it out of the body.

The most attention of black foods is the antioxidant activity that removes free radicals. Anthocyanins, a representative antioxidant of black foods, suppress the increase of blood pressure, improve liver function, improve vision, and absorb excess lipids that cause obesity. Recent studies have shown that it has excellent effects on inhibition, skin beauty, and the like.

An object of the present invention is to provide a herbal composition for preventing hair loss and promoting hair growth, and a method of manufacturing the same.

In order to achieve the above object, the present invention prevents hair loss containing black rice extract, black bean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract And it provides a herbal composition for promoting hair growth.

According to one embodiment of the present invention, the black rice extract, black bean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract is the distillation of each hot water extract It can be liquid.

According to one embodiment of the present invention, black rice extract, black bean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract, respectively, 10 to 30 parts by weight, 40 -60 weight part, 20-40 weight part, 10-30 weight part, 10-30 weight part, 10-30 weight part, 10-20 weight part, 10-20 weight part, 1-10 weight part, and 1-10 weight part It may be part by weight.

In another aspect, the present invention provides a method for producing a herbal composition for preventing hair loss and promoting hair growth, comprising the following steps:

(a) hot water extraction by mixing black rice, black beans and black sesame seeds, hot water extraction by mixing mulberry, grapes, thorns, plums, bokbunja, blueberries and black olives, and then cooled the steam produced in each hot water extract Obtaining each distillate; And (b) mixing each of the distillates in a ratio of 1-3: 3-1.

According to one embodiment of the invention, the black rice, black beans and black sesame seeds may be mixed in 10 to 30 parts by weight, 40 to 60 parts by weight and 20 to 40 parts by weight, respectively.

According to one embodiment of the present invention, the mulberry, grapes, thorns, plums, bokbunja, blueberries and black olives are 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 20 parts by weight, respectively. , 10 to 20 parts by weight, 1 to 10 parts by weight and 1 to 10 parts by weight can be mixed.

The herbal composition for preventing hair loss and promoting hair growth according to the present invention has no cell stimulation against human-derived keratinocytes, and is effective for preventing hair growth and wool, and hair loss through a strong antioxidant activity, and especially general hot water extraction is constant temperature. The essential oil components, aromatic components, and antioxidant components, which are volatilized and lost, are recovered to the cooling coil during the hydrothermal extraction process, thereby preventing hair loss and promoting hair growth through strong antioxidant activity.

Hereinafter, the herbal composition for preventing hair loss and promoting hair growth in accordance with the present invention will be described in detail.

The present invention is a black rice extract, black bean extract, black sesame extract, mulberry extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract containing herbal for preventing hair loss Regarding the composition, preferably, the black rice extract, black bean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract is the distillation of each hot water extract It may be liquid.

In one embodiment, the herbal composition according to the present invention is a composition in which the distillates of the hot water extracts are mixed, or hot water extract by mixing black rice, black beans and black sesame seeds, and then obtain a distillate therefrom, AUDI, grapes, Prickly pear, plum, bokbunja, blueberries, black olives may be mixed with the hot water extract, and then the distillate is obtained therefrom and the two distillates may be mixed.

Specifically, the herbal composition of the present invention, black rice extract, black soybean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract, respectively, 10 to 30 parts by weight, 40 -60 weight part, 20-40 weight part, 10-30 weight part, 10-30 weight part, 10-30 weight part, 10-20 weight part, 10-20 weight part, 1-10 weight part, and 1-10 weight part It is contained in the range of parts by weight, and if it is below or above the content range of each of the components, it is not preferable because the antioxidant activity is reduced by reducing the production of free radicals.

Black rice (black rice), which is a component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, has significantly higher protein, fat, ash content and total dietary fiber content than normal brown rice, and unsaturated fatty acid (linoleic acid), Na Mineral content of, Ca, Fe, K, etc. is also higher than that of ordinary brown rice. In addition, it contains a large amount of anthocyanin pigment, which is not found in ordinary rice, and contains a substance called selenium, which prevents cancer, and has excellent anti-cancer and anti-aging effects. In addition, mineral elements contain more than five times as much as ordinary rice, and can consume various trace elements evenly. Black rice enhances the body's regulating and immune functions, preventing skin care, white hair, preventing and aging, and various diseases. Effective for prevention

Common sense of black rice every day is to improve and strengthen the body's comprehensive regulatory and immune function to prevent aging, disease prevention and women's beauty. In addition, the black pigment of black rice is water-soluble, so it is easy to be absorbed by the human body, especially those who have a lot of gray hair at a young age can be expected to use good for the prevention and treatment of white hair. In particular, black rice is known to promote the secretion of HGF, one of the hair growth factors, to thicken the follicles (the lower end of the hair) and to make the hair grow thicker.

It is known that black soybean, which is one component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, has an ingredient that facilitates blood circulation of peripheral blood vessels and is effective in gray hair and hair loss symptoms. That is, it contains many unsaturated fatty acids which are inhibitors of 5-α-reductase, which causes hair loss. Prevents cholesterol buildup in blood vessels and cleans them just like saponins The unsaturated fatty acid has been reported by the University of Sikako that essential fatty acids such as gamma linoleic acid, alpha linoleic acid and oleic acid found in vegetable oils inhibit both types 1 and 2. Black soybeans belong to the water in Yin and Yang, and make the function of the kidneys and bladder smooth. In oriental medicine, the "detoxification ability is especially good, and the damaged human tissue is rapidly restored." Arginine (arginine), a nutrient necessary for hair growth, is an amino acid contained in black soybeans. It is a metabolic precursor of Nitric Oxide that promotes hair growth, and it is essential for hair growth. Cysteine, a component, has been shown to be effective in preventing hair loss. Particularly good for white hair and hair loss is because black soybeans have ingredients that facilitate the circulation of peripheral blood vessels. Vitamin E or unsaturated fatty acids in black soybeans can dilate blood vessels and improve blood circulation.

Also, in China, hair is called extra blood. In order to improve the quality of hair, it has been considered important to strengthen the liver and improve blood circulation, which controls the life energy spoken in Chinese medicine. As the age of the liver decreases, the worry about hair loss and gray hair increases. Black beans are effective in eliminating these concerns.

Black sesame, which is one component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, has been regarded as black sesame, which is black sesame among the sesame seeds in China since ancient times, and is treated as a good medicine. come. Black sesame seeds contain good protein, fat, calcium, vitamins B ₁ , B ₂ , and E. Calcium contains twice as much content as white sesame seeds and is effective in relieving constipation and adult diseases such as arteriosclerosis, high blood pressure and diabetes. Black sesame seeds are good for gastrointestinal disorders and anemia, especially to keep your hair healthy and shiny. The protein contained in black sesame is a raw material of keratin, the main ingredient of hair, and nourish the scalp to prevent hair loss. In particular, the essential amino acid called tryptophan, which is contained in large amounts of black sesame seeds, keeps the skin clean and shiny. In addition, black sesame seeds contain lipids, which strengthen blood vessels and affect scalp health, which is very effective in preventing hair loss, and the anti-aging ingredient gamma tocopherol (vitamin E) contained in black sesame seeds increases antioxidant activity. Excellent chemical action, especially if you are bald early.

In oriental medicine, the blood is broken and hair loss occurs. Therefore, black sesame seeds that supplement blood are effective in preventing hair loss and regenerating black hair. Black sesame seeds contain amino acids such as cystine and tyrosine, which are necessary for hair growth, and are rich in protein. It also contains fatty acids, such as oleic acid and linoleic acid, which can cause skin and hair to shine.

One component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention is mulberry fruit (alcohol fruit) is contained evenly in vitamins, minerals and the like little organic acid. Audi contains less sugar, calcium, iron, and vitamin C than cherries and cherries, but it also contains more protein, fat, fiber and B-complex. Dizziness and head counting early, and the skin complexion due to blood thinning is excellent.

Grape, which is one component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, contains a lot of sugar (glucose) as its component, which is good for fatigue recovery, and is rich in vitamins A, B, B2, C, D, etc. Boost metabolism Other minerals such as calcium, phosphorus, iron, sodium and magnesium are also included. Alkaline foods help strengthen muscles and bones and help diuretic edema. In addition, it acts as a blood and hematopoietic effect is good for anemia, inhibits viral activity to prevent tooth decay, and also helps to prevent degenerative diseases such as resveratrol and Alzheimer's disease and Parkinson's disease.

One component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention is thorny ginseng (Siberian ginseng), which is an arboraceae plant such as ginseng, which has long been classified as a medicinal herb that has no toxicity and side effects in the oriental region (herbal). Was used as a medicine. Its main components are 7 types of glycosides of triterpenoids, which are ereuterosides A, B, C, D, E, F, and G. Other sugars such as glucose and galactose, and large amounts of carotene, vitamin B1, B2, and C minerals It contains abundantly. It also contains glycosides such as sterol, coumarin, ligunin, flavones, and futarein. Stell has the effect of stimulating sex hormones, coumarin has a significant calming effect. In particular, flavones have the effect of expanding the coronary arteries and increasing the amount of blood, through the expansion of the blood vessels to facilitate the supply of oxygen to help prevent hair growth and hair loss.

Plum, one component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, is a report of vitamins and minerals. In addition to vitamins A, B, and C, potassium, phosphorus, calcium, magnesium, copper, iron, manganese, sodium, and carbohydrates, fats, proteins, and fiber are all in balance, making it effective for skin care. Plum is an alkaline food with high content of potassium, calcium, and sodium, and is an excellent pharmaceutical food containing a large amount of anticancer ingredient called amigdarin, which is found in apricots, plums, apples, etc., and has a high content of antioxidants.

Bokbunja, one component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, refers to the immature fruit of the bokbunja strawberry belonging to the rosaceae. Bokbunja are used when the oil well (몽 精), dream tablets (유 精), urination (또는) or urine frequently due to weak kidney function and lack of yanggi. It also brightens the eyes, lightens the body, prevents whitening of the hair, and evens the urine, while at the same time makes the skin soft and beautiful. The components of Bokbunja are organic acids containing tartaric acid and citric acid, and essential oils, sugars, ash, vitamins A and B, and vitamin C.

Resveratrol, which is contained in a large amount of blueberries as one component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, has a direct antioxidant effect and cyclooxygenase which is several times higher than vitamins C and E. It has an anti-inflammatory effect by inhibiting the activity of proinflammatory enzymes such as lipoxygenase.

Black olive, which is a component of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, contains a large amount of unsaturated fatty acids and antioxidants such as vitamin E, tocopherol, etc., which has an excellent effect on preventing aging. Vitamin E and provitamin in olives help prevent skin aging and release waste and toxins to keep your skin clear and radiant. Olive also has its own antiseptic cleansing properties, so if you apply it lightly where the skin trouble has occurred, it will calm the trouble.

Oleic acid is a sebum component that makes up 60% of human skin. It penetrates into the hair and scalp and is excellent for protecting the hair and scalp for a long time. In addition, oleic acid, which has a high affinity for the human body, forms a thin film on the hair to prevent loss of moisture and has excellent hair gloss effect due to high refractive index.

In addition, the present invention (a) hot water extract by mixing black rice, black beans and black sesame, hot water extract by mixing the mulberry, grapes, thorny oak, plum, bokbunja, blueberries and black olives, and then produced in each hot water extract Cooling the vaporized steam to obtain each distillate; And (b) provides a method of producing a herbal composition for preventing hair loss and hair growth promoting comprising the step of mixing each of the distillate in the ratio of 1 to 3: 3 to 1.

As an example of the hot water extraction process, it can be heated to 80 to 100 ℃ for about 8 to 48 hours in a boiling water distillation to obtain a hot water extract, mixing each distillate in the ratio of 1 to 3: 3 to 1 Preferably, it can be mixed in a ratio of 1: 1.

In addition, the black rice, black beans and black sesame seeds are 10 to 30 parts by weight, 40 to 60 parts by weight and 20 to 40 parts by weight, respectively, the audio, grapes, thorns, plums, bokbunja, blueberries and black olives are each 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 20 parts by weight, 10 to 20 parts by weight, 1 to 10 parts by weight and 1 to 10 parts by weight can be mixed. If the components are above or below the content range, the antioxidant capacity is reduced, which is not appropriate.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1 Herbal Compositions for Hair Protection and Hair Growth

In order to obtain a herbal composition for preventing hair loss and promoting hair growth according to the present invention, according to the components and compositions shown in Tables 1 and 2 below, black rice, black beans, black sesame seeds, mulberry, grapes, grapes, plums, Bokbunja, blueberries and black olives were sufficiently washed in purified water, and then mixed in the content ratios indicated above.

TABLE 1

TABLE 2

Specifically, 20 g of black rice, 50 g of black beans, 30 g of black sesame seeds were used to obtain 10 L of hydrothermal extract composition of the composition shown in Table 1, and to obtain 10 L of hydrothermal extract composition of the composition of Table 2, ) 40g, grape 40g, thorny seed 40g, plum 30g, bokbunja 30g, blueberry 10g, 10g black olive was used. Each was heated at 80 to 100 ° C. for 8 to 48 hours in a boiling water distillation unit to obtain a hot water extract. A cold water cooling tube of 4 to 10 ° C. was used to obtain distilled components volatilized at the extraction temperature during the extraction process. Specifically, vapors volatilized during hot water extraction were condensed while passing through a cold water cooling tube and recovered in a hot water still. The obtained hot water extract composition was centrifuged at 4 ° C. for 30 minutes at 12,000 rpm to remove solids, and the supernatant was used for the following experiment. The hot water extractive distillate of Table 1 and the hot water extractive distillate of Table 2 were used in a 1: 1 mixture.

Experimental Example 1: Cytotoxicity test of the herbal composition according to the present invention

(1) Human keratinocyte cell culture

The human keratinocyte HaCaT cell line (Cell Line Service, Germany) was incubated in a 37 ° C., 5% CO ₂ incubator for this study. When the degree of cultivation was as large as 85 to 90% of the culture vessel, the cells were detached by trypsin treatment and counted, followed by subculture at 5 × 10 ³ cells / cm ² . Cells were cultured with Delbecco's Modified Eagle Medium (DMEM, GIBCO, Cat) supplemented with 10% fetal bovine serum (FBS, GIBCO, Cat.No., 26140-079, USA), 100 U / ml penicillin and 100 μg / ml streptomycin. No. 11995-065, USA). 75 T-flask (NUNC, Cat. No. 156499, Danmark) was used for passaging, and 24 well plates (NUNC, Cat. No., 142475, Danmark) were used for cytotoxicity testing.

(2) cytotoxicity test

MTT assay is a method of measuring the cytotoxicity of a test substance by finally forming a pigment product called formazan by using the dehydrogenation effect of mitochondria that is active only in living cells.

In order to determine whether the herbal composition for preventing hair loss and promoting hair growth according to the present invention can show hair protection and hair growth promoting effects without irritation of the scalp even during long-term use, it was tested for cytotoxicity against human keratinocytes. Human keratinocytes cultured in the above were counted in a 24-well plate at 5 × 10 ³ cells / well in the same manner and counted using a hematocytometer. When incubated for 48 hours in DMEM containing 10% FBS and incubated for 25-30% of the surface area of the culture vessel, FBS-free containing 10% to 0.1% of the herbal composition for preventing hair loss and promoting hair growth prepared in Example 1 Replaced with DMEM and incubated for 24 hours. After incubation, 50 µl of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg / ml) was added and 3 hours Further incubation. Subsequently, discard all the cell cultures, treat 200 μl of dimethylsulfoxide (DMSO, Sigma D2650, USA) with 200 μl per well, stir, and then take 100 μl of 96 wells with Enzyme-Linked Immunosorbent Assay (ELISA). Absorbance was measured at 570 nm. The degree of cytotoxicity was expressed as a percentage based on the absorbance intensity of the control group using pure water.

As shown in Figure 1, compared with the untreated group (C), as a result of treatment up to 10%, there was no statistical difference with the untreated group. Therefore, it was found that scalp stimulation due to cytotoxicity was not induced even after long term use of the herbal composition for preventing hair loss and promoting hair growth according to the present invention.

Experimental Example 2: Antioxidant Test of Herbal Compositions According to the Present Invention

Compound 1,1-diphenyl-2-picryl hydrazyl (DPPH, Sigma D9132-1G, USA) generates free radicals in ethanol, and the hydrothermal extract distillate composition for preventing hair loss and promoting hair growth prepared in Example 1 It was confirmed how much the amount of free radicals produced by mixing in a proportion.

To 0.2 ml of ethanol, 0.5 ml of 0.1 mM DPPH solution prepared with ethanol and 0.3 ml of the herbal composition prepared in Example 1 are added so that the final concentration is from 10% to 0.1%. Vortex vigorously for 10 seconds and store in cold dark for 30 minutes. Absorbance is measured at 517 nm using ELISA. The degree of antioxidant activity was expressed as a percentage based on the absorbance intensity of the control group using pure water.

As shown in FIG. 2, the DPPH free radicals of the reaction system were 42.23%, 19.09% when treated with 5%, and 2.03% when treated with 1%, compared to the control group using purified water. These results indicate that when the herbal composition for preventing hair loss and promoting hair growth according to the present invention is applied to hair products such as shampoos, hair loss prevention and hair growth are possible with a significant antioxidant activity while using a high content of 1-10%. It indicates that it can have a promoting effect.

Experimental Example 3: Determination of the Mouse Hair Growth Promotion Effect Using the Composition of the Present Invention

In order to determine the effect of the herbal composition for preventing hair loss and promoting hair growth according to the present invention, the hair growth promoting effect of the composition was confirmed using C57BL / 6N mice. On 49-51 days of age, when C57BL / 6N mice enter the second catagen phase, hair is completely removed using wax, followed by 10 mice of the herbal composition and control material of the present invention prepared in Example 1 500 μl was applied twice a day for 21 days. The conclusion was drawn by comparing the degree of hair growth with the naked eye over time. As a control, pure water having no hair growth promoting effect was used. Table 3 below is a table comparing the growth of hair according to visual observation.

[Table 3] Comparison of hair growth rate over time

In Table 3,-means that the hair is not almost grown, + means that the hair grows over time, the more the number of +, the more hair is grown. On day 8 it can be seen that the treated group grows more hair in a short time than the control group, which means that the composition according to the present invention promotes hair growth. For reference, the same number of + in the control group and the treatment group on the 21st day, since the control group also did not start to gradually enter the growth phase, it means that there is no difference with the naked eye.

In addition, Figure 3 is a photograph showing the hair growth process over time of the mouse, compared to the pure water, when 8 days and 21 days, the excellent hair growth effect appeared.

Experimental Example 4: bacteriostatic test through the remaining live bacteria test of the herbal composition according to the present invention

One of the main causes of damaged hair is unclean scalp conditions such as oxidative stress caused by pollutants such as air pollution and dandruff caused by abnormal dandruff transition.

In the herbal composition for preventing hair loss and promoting hair growth according to the present invention, it was confirmed whether bacteriostatic ability in which microorganisms do not survive without preservative treatment existed against bacteria and fungi. In order to confirm the number of viable bacteria that can be generated in the hot water extractive distillate, immediately after the preparation of the herbal composition of Example 1 and left at room temperature for 30 days, a viable cell number identification test was performed.

0.1 ml of the herbal composition prepared in Example 1 was plated on a retinal agar medium (Difco, USA) and incubated at 30 ° C. for 48 hours to confirm the presence of bacteria, and 0.1 ml of Sabrowood dextrose agar medium (Difco, USA) and incubated for 72 hours at 25 ℃ to confirm the presence of fungi.

As shown in Figure 4, immediately after the preparation of herbal compositions for preventing hair loss and promoting hair growth, the presence of bacteria (A) and fungi (B) was confirmed by the method of culturing after plating on agar medium, and there were no live bacteria. After 30 days at room temperature, bacteria (C) and fungi (D) did not exist.

Therefore, the bacteriostatic ability to inhibit the transition of microorganisms without the addition of preservatives even at long-term storage at room temperature is the herbal composition for preventing hair loss and promoting hair growth according to the present invention by inhibiting abnormal transitions, such as dandruff production, hair loss prevention and hair growth promoting effect Will be possible.

1 is a graph measuring non-cytotoxicity against human keratinocytes of the herbal composition for preventing hair loss and promoting hair growth according to the present invention.

Figure 2 is a graph confirming the effect of the antioxidant activity of the herbal composition for preventing hair loss and promoting hair growth according to the present invention as free radical scavenging ability of DPPH.

3 is a photograph showing the hair growth process over time after administration of the herbal composition for preventing hair loss and promoting hair growth according to the present invention.

Figure 4 is a photograph showing the bacteriostatic ability of the herbal composition for preventing hair loss and promoting hair growth in accordance with the present invention. (A and B: bacteria and fungi immediately after preparation of the herbal composition; C and D: bacteria and fungi after standing for 30 days at room temperature).

Claims (6)

Herbal composition for preventing hair loss and promoting hair growth, comprising black rice extract, black bean extract, black sesame extract, mulberry extract, grape extract, prickly pear extract, plum extract, bokbunja extract, blueberry extract and black olive extract. According to claim 1, wherein the black rice extract, black bean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract is the distillate of each hot water extract Herbal composition for preventing hair loss and promoting hair growth. According to claim 1 or 2, black rice extract, black soybean extract, black sesame extract, audi extract, grape extract, thorny opi extract, plum extract, bokbunja extract, blueberry extract and black olive extract, respectively, 10 to 30 parts by weight, 40 to 60 parts by weight, 20 to 40 parts by weight, 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 20 parts by weight, 10 to 20 parts by weight, 1 to 10 parts by weight and 1 to Herbal composition for preventing hair loss and promoting hair growth of 10 parts by weight. Method of producing a herbal composition for preventing hair loss and promoting hair growth comprising the following steps: (a) Hot water extraction by mixing black rice, black beans and black sesame seeds, hot water extraction by mixing audi, grapes, kaoshigapi, plums, bokbunja, blueberries and black olives, and then the steam produced in each hot water extract Cooling to obtain each distillate; And (b) mixing each of the distillates in a ratio of 1-3: 3-1. The method of claim 4, wherein the black rice, black beans and black sesame seeds are mixed 10 to 30 parts by weight, 40 to 60 parts by weight and 20 to 40 parts by weight, respectively. The method of claim 4, wherein the mulberry, grapes, grapes, plums, bokbunja, blueberries and black olives are 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 30 parts by weight, 10 to 20 parts by weight, 10 to 10 parts by weight, respectively. 20 parts by weight, 1 to 10 parts by weight and 1 to 10 parts by weight of a herbal composition for preventing hair loss and promoting hair growth.

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