KR20090081178A - Cosmetic composition containing wild flower extracts - Google Patents

Abstract

A composition containing wild flower complex extracts is provided to have excellent synergic effect than single extract and cell viability of fibroblast. A cosmetic composition containing an wild flower extracts comprises a Cinquefoil extracts, Elsholtzia splendens extracts, and Hydrangea extracts and Daffodil extracts. The content of Cinquefoil extracts, Elsholtzia splendens extracts, and Hydrangea extracts and Daffodil extracts is each 20 to 40 weight%. The extract is contained in 0.01 to 50 weight% based on the total weight.

Description

Cosmetic composition containing wild flower extract {Cosmetic composition containing wild flower extracts}

The present invention relates to a cosmetic composition having various useful effects, characterized in that it contains a wildflower extract.

Most life on earth uses energy obtained by breathing and oxidizing oxygen in the air to maintain life. Toxic substances that inevitably destroy cells in the metabolic processes that require oxygen are made as by-products and are called free radicals. Free radicals attack bodily tissues to oxidize and damage cells, and are also called harmful oxygen. On the other hand, a large amount of free radicals such as O ₂ and H ₂ O ₂ also occurs in the biological defense process to remove the pathogen or foreign substances, and also act to protect the human body from the pathogen through their strong sterilization action. Free radicals may cause damage to cells or organelles, and oxidize amino acids of various proteins in vivo, leading to a decrease in protein function. It also damages nucleic acids, resulting in modification of nucleic acid bases, release of nucleic acid bases, cleavage of bonds, and oxidative degradation of sugars, which can cause mutations and cancer.

Free radicals can attack cell membranes to produce metabolic waste material called lipofuscin. The increased amount of lipofuscin in the body leads to 'aging spots', which means that a lot of metabolic debris is produced by cell destruction. Lipofuscin, in turn, adversely affects cells' ability to repair and regenerate damage, disrupting DNA / RNA synthesis and protein synthesis and destroying cellular enzymes necessary for important chemical metabolism. The damage caused by free radicals starts from birth until death. In young age, the body has a wide recovery and substitution mechanism, so its effect is relatively small. However, as we age, the damage caused by free radicals increases and their effects accumulate, while the antioxidant capacity against free radicals declines, aging the cells.

In addition, as the skin ages, the skin becomes stained and dull, and blemishes such as blemishes and freckles are produced, and there are many efforts to improve the skin. As the skin ages, the skin sags, wrinkles occur, and the skin dries. Many efforts to improve these symptoms due to aging has emerged as a great concern in the cosmetic manufacturing industry, for which multifaceted efforts have been made to include extracts of certain substances using natural products as ingredients of cosmetics.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition that can improve various symptoms caused by aging of the skin.

More specifically, the present invention is to provide a cosmetic composition having an excellent antioxidant effect, the effect of skin whitening, wrinkle improvement, skin moisturizing for improving various problems caused by aging of the skin.

In order to achieve the above technical problem, the present invention provides a cosmetic composition for antioxidant, skin whitening, skin wrinkle improvement or moisturizing, characterized in that it comprises a hydrangea extract, flower fragrance extract, hydrangea extract and narcissus extract.

More preferably, the present invention provides a cosmetic composition, characterized in that the content of each of the yangji extract, flower fragrance extract, hydrangea extract and narcissus extract in the cosmetic composition is 20 to 40% by weight relative to the total extract weight.

Hereinafter, the cosmetic composition and the preparation method thereof according to the present invention will be described in more detail.

The cosmetic composition according to the present invention comprises a birch flower extract, flower fragrance extract, hydrangea extract and narcissus extract as an active ingredient, the cosmetic composition comprising these wildflower extracts as an active ingredient has an excellent antioxidant effect, skin whitening effect, skin wrinkle improvement effect And moisturizing effect.

The present invention is also based on the surprising finding that the antioxidant, skin whitening, wrinkle improvement and moisturizing effects, which are useful as cosmetics when mixed with the wildflower extracts alone, are synergistically increased.

Extracts included in the cosmetic composition according to the present invention may be obtained by mixing the extracts obtained by separately extracting each of the fern, flower fragrance oil, hydrangea and narcissus as an extraction solvent, preferably the fern, flower fragrance oil, hydrangea and narcissus mixture It can be obtained by extracting all at once with the extraction solvent.

Yellow flower used in the present invention ( Potentilla fragarioides ) is a perennial herbaceous plant belonging to the Rosaceae family, growing well in the sun, and distributed in Korea, Japan, and China. In oriental medicine, the outpost is called Cheon-na (, 子 筵) and the root is called Cheon-geun, and it is known to have a hemostasis effect (primary natural drug metabolism, Namsandang, 1989, p423). (+)-Catechin, quercitrin, caffeic acid, isoquercitrin, 4-ο-caffeoyl-L-threolic acid , Quercetin-3-ο-beta-D-glucopyranosyl-beta-D-xylopyranoside has been reported separately, and (+)-catechin has an antioxidant effect The Journal of Pharmacognosy, 29, 1998, p79.

Flower fragrance oil ( Elsholtzia) used in the present invention splendense) is a perennial plant that grows mainly in the mountains of Japan, China, Siberia, Mongolia and elsewhere, including South Korea to Lamiaceae (Labiatae) plants. It contains about 1% of essential oils and has a strong scent. The main ingredient is elscholtziadeton and other sesquiterpenes. Herbal medicine is used as a herb, herbal medicine is called spice oil (야), Yaso (野 蘇), it has a expectorant effect and diuretic effect, antipyretic effect, antibacterial effect, and is known to be effective in systemic edema. (Pesticide Information, 15, Korea Pesticide Industry Association, 1994, p86; Dosul Oriental Medical Medicinal Dictionary, Seoul, Dongdo Culture History, 1984, p102; Primary Color Book of Korea) 3rd, Kyohaksa, 2000, p21).

Hydrangea used in the present invention macrophylla ) is an ornamental shrub belonging to the family Saxifragaceae. The color of the flower changes from neutral to white, acidic to blue, and alkaline to pink, depending on the soil. The outpost is called Palangea (八仙 花), medicinal, and the effect of treating the crane, cardiac calorimetry (心 熱 悸), cystic wind (번 風) and swelling (煩燥) is known. Alkaloids have antimalarial effects on leaves and flowers, rutin on flowers, daphnetin and umbelliferone, hydrangenol, hydrangenic acid, Lunurular acid (lunularic acid), the leaves contain skimmin (skimmin), etc. (Korean medicinal plants, Kyohaksa, 2006, p205; Ingredients and use of herbs, January Seokseok, 1999, p318).

Narcissus used in the present invention tazetta is a perennial genus belonging to the family Amaryllidaceae, whose flowers bloom in white to December. It does not bear fruit and multiplies with scales. Native to the Mediterranean coast, it is a naturalized plant grown all over the country. Roots are called narcissus root (水仙 根), small species (消腫), drainage (排膿) efficacy, and carbuncles, changsok and poisonous snakes. The scales, leaves and flowers include lycorine, galanthamine and tazettine, and the flowers have narcissin. There is a strong excitatory effect on the uterus, antitumor action and antiviral action on sarcoma and ascites cancer. In the private sector, it is used to crush the scales, and juice is effective for eye diseases (Korean medicinal plants, Kyohaksa, 2006, p549; Medicinal ingredients and uses, January psalm, 1999, p817).

The present invention also provides a method for producing a cosmetic composition comprising a fern, scented oil, hydrangea and narcissus extract. Other wild flower extracts according to the present invention is purified water for a certain amount of yangji flower, flower fragrance oil, hydrangea and narcissus mixed material or a certain amount of yangji flower, flower fragrance oil, hydrangea and narcissus; Methanol, ethanol, propyl alcohol, butyl alcohol, which are lower alcohols having 1 to 4 carbon atoms; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; Alternatively, they may be prepared by extracting the mixed solvent by adding 1 to 20 times the volume, and then selectively concentrating or purifying. More preferably, extracting the cosmetic active ingredient by adding an extraction solvent as described above (a) heating for 4 to 20 hours at a temperature of 50 to 95 ℃ in a state that the cooling condenser is installed to prevent the solvent from evaporating It is preferable to extract by dipping, or (b) by dipping for 1 to 15 days at a temperature condition of 5 to 37 ℃.

In the cosmetic composition according to the present invention, in order to synergistically improve the useful effects as the cosmetics described above, the content of each of the hydrangea extract, the fragrance oil extract, the hydrangea extract and the narcissus extract is preferably 20 to 40% by weight based on the total extract weight. In addition, the cosmetic composition according to the present invention more preferably comprises an extract obtained by extracting the mixture containing 20 to 40% by weight of each of the yangyang flowers, fragrance oil, hydrangea and narcissus relative to the total weight of wildflowers.

In addition, the wildflower extract as an active ingredient in the cosmetic composition of the present invention is preferably included in an amount of 0.01 to 50% by weight relative to the total weight of the cosmetic composition. If the content of the extract is less than 0.01% by weight, it is not desirable to achieve a sufficient amount of useful effects, that is, antioxidant, skin whitening, skin wrinkle improvement or moisturizing effect, which are originally desired, and the content of the extract as an active ingredient is total. When it exceeds 50% by weight relative to the weight of the cosmetics may cause a problem in the stability of the cosmetics, and thus there is a concern that the cosmetic appearance or sufficient cosmetic effects may not be expressed when using the cosmetics.

The cosmetic composition according to the present invention may be prepared and commercialized in the formulation of creams, packs, lotions, essences, lotions, foundations or makeup bases, but is not limited thereto.

The extract obtained from the wildflower mixture containing yangji flower, scented oil, hydrangea and narcissus according to the present invention using the extraction solvent showed a very useful effect as a cosmetic such as antioxidant effect, skin whitening effect, wrinkle improvement effect, moisturizing effect, etc. In particular, the mixed extract obtained from the mixture showed a superior synergistic effect than the single extract. In addition, the extract according to the present invention was excellent in cell viability of the fibroblasts even at high concentrations.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

<Examples 1 to 4>

250 g of selected hydrangea, 250 g of hydrangea, 250 g of flower fragrance oil, and 250 g of daffodil, respectively, were weighed into an extractor, and 5 kg of an extraction solvent (30% hydrous butylene glycol) was added thereto, followed by immersion at room temperature for 6 days, followed by 400 mesh filter cloth. After filtration and left at 4 ° C. for 7 days to mature at low temperature, it was filtered secondly with a 0.45 um filter. The extract filtrate was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor.

<Example 5>

After weighing 62.5g of selected hydrangea, 62.5g of hydrangea flower, 62.5g of flower fragrance oil and 62.5g of narcissus, put it in the extractor, add 5kg of extraction solvent (30% hydrous butylene glycol), and extract it by dipping at room temperature for 6 days. After filtration with 400 mesh filter cloth, the mixture was left at 4 ° C. for 7 days to mature at low temperature, followed by secondary filtration with a 0.45 um filter. The extract filtrate was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor.

division Extraction Sample Example 1 Sunny flower Example 2 Flower fragrance Example 3 hydrangea Example 4 daffodil Example 5 Sunny day, hydrangea, balm, narcissus

Experimental Example 1 Evaluation of Antioxidant Effect

Free radical scavenging activity was conducted to investigate the antioxidant effect of the active ingredient of the cosmetic according to the present invention. Free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303, 1993), and stable free radicals DPDP (1,1-diphenyl-2-picryl hydrazyl, Sigma) reagent was used.

First, each sample obtained according to the above extraction example is diluted in methanol with 1 mL of 0.2 mM DPPH solution, and mixed in 2 mL of methanol at an appropriate concentration, and the absorbance is measured at 517 nm after being left at room temperature for 10 minutes. On the other hand, the control in the free radical elimination test was set to obtain the color correction value for each of the examples by adding methanol instead of the sample solution, and put methanol instead of the DPPH solution.

By using the following equation 1 to calculate the free radical removal rate as a numerical value is shown in Table 2 below. In Table 2, SC ₅₀ is the concentration of the sample required to achieve 50% free radical scavenging ratio, which is a numerical value used in comparison with other component substances, and the smaller the value, the higher the scavenging ratio.

Free radical scavenging experiment was a modification of the method of Noro et al. (Chem. Pharm. Bull., 31, 3984 ~ 3987 (1983)), and superoxide anion scavenging activity was evaluated by xanthine / xanthine. The change in absorbance due to oxidation of nitroblue tetrazolim (NBT) by active oxygen was measured using an active oxygen generation system by oxidase.

2.4 mL of Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA, 0.1 mL of BSA, 0.1 mL of NBT, and 0.1 mL of sample were added thereto, mixed well, and allowed to stand at 25 ° C. for 10 minutes. After adding 0.1 mL of xanthine oxidase and reacting at 25 ° C. for 20 minutes, 6 mM CuCl ₂ was added to stop the reaction. Absorbance was measured at an optical wavelength of 560 nm. Meanwhile, the control group in the active oxygen scavenging activity test was set in the same manner by adding purified water instead of the sample solution, and was added to the purified water instead of xanthine oxidase solution to obtain a color correction value for each example.

By using the following equation (2) to calculate the oxygen free radicals are shown in Table 2 below. In Table 2 below, IC ₅₀ is a concentration of the sample required to achieve 50% of the active oxygen scavenging ratio, which is a value used when comparing with other component substances. The smaller the value, the higher the scavenging ratio.

division SC ₅₀ (mg / mL) IC ₅₀ (mg / mL) Example 1 0.078 0.940 Example 2 2.667 over 10 Example 3 0.422 2.422 Example 4 4.094 50 or more Example 5 0.042 0.383

As shown in Table 2, Examples 2 to 4 except Example 1 of the single extract did not have good antioxidant power. On the other hand, in the mixed extract Example 5, the antioxidant power capable of scavenging free radicals and free radicals was significantly increased compared to Examples 2 to 4, which are the single extract examples, and the antioxidant power was about 2 times higher than that of Example 1. .

Experimental Example 2 Evaluation of Inhibition and Improvement of Skin Wrinkles

Elastase Inhibition Activity Test was carried out to test the inhibition and improvement effect of the skin wrinkles of the active ingredient of the cosmetic according to the present invention.

A method for measuring the activity of elastine, an enzyme that degrades elastin, was used to determine the color change caused by the degradation of ρ-nitroaniline using N-Succinyl- (Ala) ₃ ρ-nitroaniline, an elastay substrate. It is a method of measuring elastase activity by measuring absorbance at a wavelength of nm. PH 8.0, 0.267 M Trizma-HCl (Sigma), substrate solution 8.8 mM N-Succinyl- (Ala) ₃ ρ-nitroaniline (Sigma), enzyme solution 10 ug / mL (Sigma) Used as a concentration. 60 uL of buffer solution, 20 uL of substrate solution and each sample obtained from the extraction examples 1 to 5 were dissolved in purified water for each concentration, and then mixed with 100 uL of sample solution. The amount of p-nitroaniline produced was measured at a wavelength of 405 nm using a microplate reader. On the other hand, the control for measuring the elastase activity was measured in the same way to put the purified water instead of the sample solution, it was set the case of obtaining a color correction value for each of the purified water instead of the enzyme solution.

Elastase inhibition rate was calculated as a numerical value using Equation 3 shown in Table 3 below, the numerical value shown in Table 3 is expressed as the inhibition rate for the sample 1 mg / mL.

division % Inhibition Example 1 10.398 Example 2 2.581 Example 3 No effect Example 4 7.917 Example 5 25.806

As shown in Table 3, Examples 1 to 4 using the single wildflower did not show an excellent effect in the elastase activity inhibition test, but in the mixed extract Example 5, improved inhibition of the elastase activity compared to single extracts It showed an effect.

Experimental Example 3 Evaluation of Whitening Effect

In order to confirm the whitening effect of the active ingredient of the cosmetic according to the present invention, the whitening effect was determined by looking at the degree of inhibition of the function of an enzyme called tyrosinase.

Tyrosinase is an enzyme that accelerates the oxidation of tyrosine in vivo and helps melanin production. In this experimental example, the method of inhibiting the function of this enzyme to inhibit the oxidation of tyrosine to form a black polymer called melanin (Pomerantz SH, J. Biochem., 24: 161-168, 1966) was applied. The whitening effect was determined.

Tyrosinase inhibitory activity of each sample was warmed up at 37 ° C for 10 minutes after adding 0.9 mL of sample, 1.0 mL of 0.1 M phosphate buffer (pH 6.8) and 1.0 mL of 1.5 mM L-tyrosine solution. I was. 0.1 mL of mushroom tyrosinase (1,500 units / mL) was added and reacted at 37 ° C. for 10 minutes, and then the absorbance was measured at 475 nm using a UV-Vis spectrophotometer to measure the inhibition rate against tyrosinase. On the other hand, the control group for the tyrosinase inhibitory test was set in the case where a buffer solution was put in place of the sample solution and measured in the same manner, and each color correction value was obtained by putting the buffer solution instead of the tyrosinase.

To calculate the tyrosinase inhibition rate using the following Equation 4 as shown in Table 4, the numerical value shown in Table 4 is shown as the inhibition rate for the sample 1 mg / mL.

division % Inhibition Example 1 27.103 Example 2 1.689 Example 3 No effect Example 4 19.209 Example 5 34.150

As shown in Table 4, in the extract example except Example 1, the effect of inhibiting tyrosinase activity was insignificant. However, the mixed extract Example 5 showed a very improved tyrosinase inhibitory effect compared to Examples 2 to 4, which are the single extraction examples, and showed an improved effect than Example 1, showing the usefulness as a whitening cosmetic.

Experimental Example 4 Safety Evaluation

In order to verify the primary safety as a cosmetic of the active ingredient according to the present invention, normal human fibroblasts were cultured and cell viability was performed by MTT assay method.

Normal human fibroblasts used in this experiment were used as a pre-sale from Cocoid Biopharm. Cells were inoculated at a density of 1 × 10 ⁴ cells in DMEM (Dulbecco's Modified Eagle's Medium) with 10% bovine serum and incubated at 37% for 5% CO ₂ for one day. Extract samples were treated by diluting them in media containing no serum for each concentration. After culturing for 1 day, MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide), a dye for cell staining, was treated and further cultured for 4 hours. The chromosome crystals were dissolved in dimethyl sulfoxide and measured for absorbance at 540 nm for relative comparison. As a control, a culture medium without the extract sample was used. Using the following equation (5) to calculate the cell viability as a number, the relative cell proliferation effect when the cell growth rate (%) of the control group without the extract sample to 100 is shown in Table 5 below.

division Cell survival rate (%) 10 ug / mL 100 ug / mL 1 mg / mL Example 1 90.594-95.251 96.724-98.889 103.182 ~ 105.112 Example 2 88.455-89.841 81.394 ~ 85.354 89.477-101.221 Example 3 84.424 to 86.212 82.129-84.635 92.090 ~ 95.264 Example 4 84.099 ~ 85.421 96.778 to 98.559 115.309-117.254 Example 5 85.947-89.644 90.089-95.627 92.643 ~ 95.259

As shown in Table 5, Examples 1 to 5 showed a fibroblast viability of 90% or more up to a concentration of 1 mg / mL, which indicates that the examples have excellent safety against fibroblasts.

<Formulation Example 1>

The composition of the flexible cosmetics containing the active ingredient material of the cosmetic according to Example 5 was prepared as shown in Table 6 below. At this time, the unit of component content is weight%. On the other hand, among the components identified by each component number in Table 6 below, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 6 were added, and components 8 to 10 were dissolved in component 7. And component 11 were added in order to prepare a mixture. As a comparative formulation example, the rest of the component constitution except for component 11 and the preparation method thereof were performed in the same manner.

number ingredient Formulation Example 1 Comparative Formulation Example 1 One Purified water Remaining amount Remaining amount 2 Carbomer 0.1 0.1 3 Hydroxyethyl cellulose 0.1 0.1 4 Butylene Glycol 2.0 2.0 5 glycerin 1.0 1.0 6 Fiji-1500 0.5 0.5 7 ethanol 8.0 8.0 8 Polyoxyethylene Cured Castor Oil 0.2 0.2 9 Triethanolamine 0.1 0.1 10 antiseptic a very small amount a very small amount 11 Active ingredient of the cosmetic according to Example 5 10.0 -

<Formulation Example 2>

The composition of the elastic lotion containing the active ingredient of the cosmetic according to Example 5 was prepared by configuring as shown in Table 7 below. At this time, the unit of component content is weight%. On the other hand, among the components identified by the component numbers in Table 7 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 8 to 11 were dissolved and dispersed in component 12, and emulsified in those heated to 70 ° C. . Thereafter, the emulsified product was neutralized with component 13, cooled to a temperature of 56 ° C, and then component 14 was added thereto, stirred, and cooled to room temperature.

number ingredient Formulation Example 2 Comparative Formulation Example 2 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate / Pig-100stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 4.0 4.0 7 Apricot Kernel Oil 4.0 4.0 8 glycerin 5.0 5.0 9 Carbomer 0.1 0.1 10 Xanthan Gum 0.03 0.03 11 antiseptic a very small amount a very small amount 12 Purified water Remaining amount Remaining amount 13 Arginine 0.1 0.1 14 Active ingredient of the cosmetic according to Example 5 10.0 -

Experimental Example 5 Evaluation of Skin Moisturizing Effect

The skin moisturizing power was evaluated by measuring the skin conductivity using a skin moisture meter (Corneometer, Courage + Khazaka, GmbH, Germany) as a clinical test for skin moisturizing the active ingredient of the cosmetic according to the present invention.

Moisturizing power measurement by skin moisture meter is a device that measures the moisture content of skin surface (e.g. stratum corneum) by capaccitance measurement method. It has the advantage of maintaining a constant measurement level of 30 ~ 40 um below the probe sensor contact.

Skin moisture measurement was carried out in a 2 × 2 cm area using the skin moisture meter under the constant temperature and humidity conditions maintained at room temperature of 20-25 ° C and relative humidity of 40-55%. 7.5 uL of Example 2 was applied and measured over time. In addition, one measurement site was measured five times each and displayed as an average value. Using the following equation (6), the skin conductivity increase rate (%) as a moisturizing power was calculated by the numerical value. In addition, the skin conductivity was measured and defaulted before application of the sample, and the control group was not applied to both the formulation example and the comparative formulation example.

Time lapse (hours) Skin Moisturizing Strength (%) Control Formulation Example 2 Comparative Formulation Example 2 0 100 100 100 0.5 112 ± 7 161 ± 8 142 ± 5 1.5 112 ± 5 127 ± 8 115 ± 4 3 105 ± 6 121 ± 10 112 ± 2 5 99 ± 3 109 ± 2 100 ± 3

As shown in Table 8, according to the application of Formulation Example 2 containing Example 5, the skin moisturizing power was increased compared to Comparative Formulation Example 2.

<Formulation Example 3>

The ingredient composition of the nourishing cream containing the active ingredient of the cosmetic according to Example 5 was prepared as shown in Table 9. At this time, the unit of component content is weight%. On the other hand, among the components identified by the component numbers in Table 9 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 9 to 12 were dissolved and dispersed in component 13 and emulsified in heating to 70 ° C. . Thereafter, the emulsified product was cooled to a temperature of 56 ° C., and then component 14 was added thereto, stirred, and cooled to room temperature.

number ingredient Formulation Example 3 Comparative Formulation Example 3 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate / Pig-100stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 8.0 4.0 7 Apricot Kernel Oil 4.0 4.0 8 Dimethicone 2.0 2.0 9 glycerin 5.0 5.0 10 Magnesium Aluminum Silicate 0.4 0.4 11 Xanthan Gum 0.03 0.03 12 antiseptic a very small amount a very small amount 13 Purified water Remaining amount Remaining amount 14 Active ingredient of the cosmetic according to Example 5 10.0 -

Experimental Example 5

In order to evaluate the skin wrinkle improvement effect and skin irritation of the cosmetic composition having various useful effects according to the present invention, a sensory test was conducted using the nourishing cream prepared in Formulation Example 3 and Comparative Formulation Example 3.

In the sensory test, the anti-wrinkle effect of skin was evaluated based on the nutritional cream of Formulation Example 3, and the anti-wrinkle effect of the nutritional cream of Comparative Formulation Example 3 was evaluated. Symptoms such as stinging and erythema were evaluated. The evaluation was performed based on the five-point method of very good (5 points), good (3 points), moderate (0 points), bad (-3 points), and very bad (-5 points). 10 is shown. In Table 10, skin irritation indicates a degree without skin irritation.

number Skin irritation Wrinkle improvement effect Formulation Example 3 Comparative Formulation Example 3 One 5 5 5 2 4 5 5 3 5 5 5 4 4 4 4 5 5 5 4 6 5 5 4 7 4 4 4 8 4 4 4 9 5 5 5 10 5 5 5 11 4 4 4 12 5 5 5 13 4 4 4 14 5 5 5 15 4 4 4 16 5 5 5 17 4 4 4 18 4 4 4 19 5 5 5 20 4 5 5 Average 4.43 4.52 4.50

As shown in Table 10, the skin irritation evaluation score of the cosmetic composition of Formulation Example 3 according to the present invention was evaluated very well at 4.43, and the skin irritation degree was low, as in Comparative Formulation Example 3. I could confirm it.

In addition, it can be seen that the relative improvement of the wrinkles of the skin of the cosmetic composition of Formulation Example 3 compared to Comparative Formulation Example 3 was 4.50, the degree of improvement is very excellent.

Claims (3)

Antioxidant, skin whitening, skin wrinkle improvement or moisturizing cosmetic composition comprising a birch extract, flower fragrance extract, hydrangea extract and narcissus extract. The cosmetic composition according to claim 1, wherein the content of each of the hydrangea extract, the flower fragrance extract, the hydrangea extract and the narcissus extract is 20 to 40% by weight based on the total extract weight. According to claim 1, wherein the extract is a cosmetic composition, characterized in that it comprises 0.01 to 50% by weight relative to the total weight of the composition.