CN111315354A - Cosmetic composition containing hydrangea macrophylla extract for resisting skin aging and keeping moisture - Google Patents
Cosmetic composition containing hydrangea macrophylla extract for resisting skin aging and keeping moisture Download PDFInfo
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- CN111315354A CN111315354A CN201880070927.9A CN201880070927A CN111315354A CN 111315354 A CN111315354 A CN 111315354A CN 201880070927 A CN201880070927 A CN 201880070927A CN 111315354 A CN111315354 A CN 111315354A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The present invention relates to a cosmetic composition for skin anti-aging and moisturizing containing hydrangea macrophylla extract, which comprises hydrangea macrophylla extract containing high concentration of hydrangeal phenol and specific ingredients, thereby having a greatly improved effect on skin anti-aging and moisturizing.
Description
Technical Field
The present invention relates to a cosmetic composition for skin anti-aging and moisturizing containing hydrangea macrophylla extract, and more particularly, to a cosmetic composition having a significant effect on skin anti-aging and moisturizing by containing hydrangea macrophylla extract containing hydrangeal phenol and a specific component.
Background
With the abundance of life, various methods have been studied to delay not only aging inside the body but also aging outside the body.
The area of greatest concern with external aging is skin. Skin aging is classified into chronological aging, i.e., intrinsic aging, which is manifested over time, and photoaging, which occurs in combination with degenerative changes occurring at sites exposed to sunlight and chronological aging.
Due to this aging process, the skin becomes dry, wrinkles appear, and the skin elasticity is lost. In the studies on the related skin aging, the most important parts are free radicals (free radial) and active oxygen (reactive species). It is reported that these occur naturally in the human body, but they also occur by exposure to sunlight or contact with various chemical substances and the like. The generated radicals or active oxygen exert oxidative stress on skin cells, and substances generated in this process are considered as causative substances for the production of melanin and wrinkles.
It has been found that, in addition to these aging-causing substances, active oxygen is exposed to polymeric matrices (collagen, hyaluronic acid, elastin) or the like that maintain the elasticity in the skin, whereby these matrices are sequentially cut.
In this regard, the following conclusions are drawn: in order to prevent skin aging, it is necessary to effectively remove generated active oxygen while controlling the generation of excessive active oxygen, and it is necessary to establish a system associated with inhibition of enzymes causing skin wrinkles, inhibition of enzymatic activities causing pigmentation, and the like.
Therefore, various studies on cosmetic compositions for skin anti-aging, whitening and moisturizing are conducted through such a related system, but there is a real situation that more effective and safe cosmetic compositions are still required.
Disclosure of Invention
In contrast, the present inventors have found that, in the course of research on various natural products, hydrangea macrophylla extracts have significant effects of anti-aging and moisturizing unlike other hydrangea macrophylla extracts, and have completed the present invention.
Accordingly, an object of the present invention is to provide an anti-aging and moisturizing cosmetic composition containing hydrangea macrophylla extract, and more particularly, to provide an anti-aging and moisturizing cosmetic composition containing hydrangea macrophylla extract containing hydrangeal phenol.
The present invention provides a cosmetic composition which is excellent and safe in storage stability and has extremely remarkable effects on aging resistance, wrinkle improvement, whitening, and moisturizing, and the cosmetic composition of the present invention is characterized by comprising an extract of hydrangea macrophylla.
Preferably, the hydrangea macrophylla extract of the present invention contains hydrangeal phenol in an area ratio of 8% or more based on high performance liquid chromatography.
From the viewpoint of having improved anti-aging, whitening and moisturizing effects, it is preferable that the hydrangea macrophylla extract of the present invention has a high performance liquid chromatography analysis result satisfying the following formula 1:
0.1＜I2/I1＜0.3(1)
in the case of the above-mentioned formula 1,
I2is prepared by subjecting hydrangea macrophylla to UV detection at 254nm in methanol solvent at a concentration of 10ug/mL, HPLC (0.1% TFA H) with C18 column at 40 deg.C and 1.0mL/min2O: 0.1% TFA MeOH ═ 40: 60) the area value of the maximum peak occurring at a retention time between 24 minutes and 27 minutes when the separation is carried out,
I1is in the following general formula I2The area value of the maximum peak that occurs under the same conditions at retention times between 34 and 36 minutes.
The cosmetic composition for skin anti-aging and moisturizing according to an embodiment of the present invention may include a mixed extract of hydrangea macrophylla and citrus peel, and it is preferable that the mixed extract of hydrangea macrophylla and citrus peel is included in an amount of 0.1 to 20 wt% with respect to the total weight of the cosmetic composition.
In the cosmetic composition according to an embodiment of the present invention, the mixed extract of hydrangea macrophylla and citrus peel is prepared by mixing 3: 7 to 7: 3 weight ratio of the coarse hydrangea and the dried orange peel.
The hydrangea macrophylla extract according to an embodiment of the present invention may be obtained by extracting with a C1-C4 alcohol or a mixture of a C1-C4 alcohol and water.
Preferably, the composition of the present invention further comprises one or more humectants selected from glycerin, butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, and propanol.
The cosmetic composition according to an embodiment of the present invention may further include one or more selected from the group consisting of a fatty material, an organic solvent, a dissolving agent, a concentrating agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic emulsifier, a filler, a metal ion-blocking agent, a chelating agent, a preservative, a vitamin, a blocking agent, a humectant, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, and a lipid vesicle.
The cosmetic composition for skin anti-aging and moisturizing of the present invention may be used for cosmetics selected from skin lotions, skin softeners, skin lotions, astringents, lotions, milk lotions, moisturizing lotions, nutrient lotions, massage creams, nutrient creams, moisturizing creams, hand creams, essences, nutrient essences, face masks, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, oils, pressed powders, loose powders, and eye shadows.
The cosmetic composition of the present invention contains hydrangea macrophylla extract containing specific components, thereby having remarkably improved effects of anti-aging, wrinkle improvement, whitening and moisturizing the skin.
In addition, the cosmetic composition of the present invention contains a mixed extract of hydrangea macrophylla and dried orange peel, and thus has not only excellent effects of anti-aging, wrinkle improvement, whitening and moisturizing the skin, but also more excellent storage stability, and further excellent skin stability due to the natural extract.
Drawings
FIG. 1 is a high performance liquid chromatogram of an extract from hydrangea macrophylla analyzed at a wavelength of 254 nm.
FIG. 2 is a high performance liquid chromatogram of an extract from the Meadowrue crispus and an extract from the Meadowrue crispus analyzed at a wavelength of 254 nm.
FIG. 3 is a graph showing the result of MMP-1 expression inhibition experiment of the hydrangea macrophylla extract of Experimental example 1 of the present invention (CON 1: negative control group for inducing MMP to express untreated UVB, CON 2: negative control group for inducing MMP to express irradiated UVB and untreated substances, RA: retinoic acid (retinoic acid) -positive control group, 12: hydrangea macrophylla extract 12ppm, 25: hydrangea macrophylla extract 25ppm, 50: hydrangea macrophylla extract 50 ppm).
FIG. 4 is a graph showing the results of experiments on moisturizing gene expression of the hydrangea macrophylla extract and the mixed extract of hydrangea macrophylla and citrus peel according to Experimental example 2 of the present invention (CON: negative control group of untreated material, 12: 12ppm of hydrangea macrophylla extract, 25: 25ppm of hydrangea macrophylla extract, 50: 50ppm of hydrangea macrophylla extract).
Detailed Description
The present invention provides a cosmetic composition having excellent effects of anti-aging and moisturizing skin, the cosmetic composition of the present invention comprising an extract of hydrangea macrophylla.
The hydrangea is used as deciduous shrub belonging to Saxifragaceae, the flower color is changed into blue, white and pink according to the acidity of soil, and flower plants are planted in various places. The root, leaf and flower of hydrangea is called hydrangea, is bitter, slightly pungent and cool in taste, contains alkaloid components with antimalarial effect, and can be used for treating heart disease, scabies and ulcerative dermatitis.
The origin of hydrangea macrophylla is korea, and roots, leaves, flowers are used as medicines, as are hydrangea macrophylla, which is a deciduous shrub belonging to the family Saxifragaceae. Deciduous and broad-leaved shrubs grow mainly in the central south at an altitude of 200-1400 m, and leaves do not fall off in winter and grow to a height of about 1 m. The bold-faced hydrangea is distinguished from the general hydrangea in that pistils and stamens develop in spite of the asexual flowers.
Although it is not clear that the cosmetic composition according to an embodiment of the present invention contains a large amount of hydrangeal phenol, it has a remarkable effect on anti-aging, whitening and moisturizing the skin by containing specific components not contained in general hydrangeal in a specific ratio to hydrangeal phenol.
In the hydrangea macrophylla extract according to an embodiment of the present invention, specific components contained in the hydrangea macrophylla other than hydrangeal phenol are not known but unknown, and are not hydrangeal acid, rutin, daphnetin, and skimmin generally contained in the hydrangea macrophylla, and correspond to the following peaks: the maximum peak occurring at retention times between 24 and 27 minutes when separated on an elid detection, philosophy (Phenomenex Luna) C18 analysis (analytical) column under 1.0mL/min conditions at a concentration of 10ug/mL in 0.1% trifluoroacetic acid water (TFA water), 0.1% trifluoroacetic acid methanol (TFA MeOH) solvent.
Preferably, the hydrangea macrophylla extract contained in the cosmetic composition according to an embodiment of the present invention may contain hydrangeal phenol, and contains the hydrangeal phenol in an area ratio of a hydrangeal phenol peak by High Performance Liquid Chromatography (HPLC) of 8% or more, more preferably 9% or more.
More preferably, the hydrangea macrophylla extract according to an embodiment of the present invention may include hydrangeal phenol in an area ratio of hydrangeal phenol peaks based on High Performance Liquid Chromatography (HPLC) of 8% or more, and include materials corresponding to 8% or more of the following peaks: the extract of hydrangea macrophylla is detected by UV detection at 254nm, 1.0mL/min, 40 deg.C, 100kgf/cm in methanol solvent at a concentration of 10ug/mL2HPLC with column under the conditions (0.1% TFA H)2O: 0.1% TFA MeOH ═ 40: 60) the maximum peak that occurs at retention times between 34 and 36 minutes when separation is performed.
Preferably, the coarse embroidered ball of one embodiment of the present invention may have a high performance liquid chromatography analysis result satisfying the following formula 1.
0.1＜I2/I1＜0.3(1)
In the case of the above-mentioned formula 1,
I2is prepared by detecting crude hydrangea in methanol at a concentration of 10ug/mL by UV at 245nm, at 40 deg.C and a concentration of 1.0mL/min and a concentration of 100kgf/cm2HPLC with column under the conditions (0.1% TFA H)2O: 0.1% TFA MeOH ═ 40: 60) the area value of the maximum peak occurring at retention times between 24 and 27 minutes when separation is carried out;
I1is in the following general formula I2The area value of the maximum peak that occurs under the same conditions at retention times between 34 and 36 minutes.
The HPLC used in the present invention may be obtained by filling Watcher 120-AP ODS column or Watcher study (Waters study) column, Waters study (Waters study) column, and others type HPLC study (Agilent 1200Series HPLC study) column, such as Purif PLC 2020Purification System, Gelsen HPLC study (Gleson PLC 2020 study) column, Hitachi HPLC study (Hitachi HPLCSs study) 36(Hitachi HPLCSs study) and others type HPLC study (Gelsen study) HPLC (Gelsen study) column, such as Waters 120-AP ODS column, or Waters study (Waters study), Waters study (Waters study) column, YMC-TriartC18, YMC-Pack ODS-A and B, thermoelectric Hypersil study (Thermosil study), Hypericum study (Aquorx study) column, Galaxc-18-Marx study (RBx study) column, such as Gerben study (Gerben study) column, Gerben study (Gelten study) column, Gerben 18C 3632, and others.
The cosmetic composition according to an embodiment of the present invention may include 0.1 to 20 wt% of the hydrangea macrophylla extract, preferably 1 to 10 wt% of the hydrangea macrophylla extract, with respect to the total weight of the composition, and preferably, the cosmetic composition according to an embodiment of the present invention may include a mixed extract of hydrangea macrophylla and citrus peel, and may include 0.1 to 20 wt%, preferably 1 to 10 wt%, with respect to the total weight of the composition.
The cosmetic composition according to an embodiment of the present invention may include a mixture of hydrangea macrophylla and dried orange peel in a ratio of 3: 7 to 7: 3, the weight ratio of the mixed extract of hydrangea macrophylla and citrus peel may be preferably 6: 4 by weight.
The hydrangea macrophylla extract according to an embodiment of the present invention may be obtained by extracting with a mixture (mixed solvent) of a C1-C4 alcohol or a C1-C4 alcohol and water.
It is preferable to extract with a mixture of C1-C4 alcohol and water, specifically, C1-C4 alcohol may be methanol, ethanol, propanol, isopropanol or butanol, and a mixed solvent of ethanol and water may be preferable.
The cosmetic composition according to an embodiment of the present invention may further include a moisturizing agent for more excellent anti-aging and moisturizing effects, and the moisturizing agent preferably combined with the hydrangea macrophylla extract of the present invention may be one or more selected from glycerin, butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, and propanol, and may preferably be butylene glycol, diol, or a mixture thereof.
The cosmetic composition according to an embodiment of the present invention may use all additives that are generally contained in the cosmetic composition, and may include, for example, one or more additives selected from the group consisting of a fatty substance, an organic solvent, a dissolving agent, a concentrating agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic emulsifier, a filler, a metal ion-blocking agent, a chelating agent, a preservative, a vitamin, a blocking agent, a humectant, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, and a lipid vesicle.
The cosmetic composition according to an embodiment of the present invention may be used for cosmetics selected from skin lotions, skin softeners, skin lotions, astringents, lotions, milk lotions, moisturizing lotions, nutritional lotions, massage creams, nutritional creams, moisturizing creams, hand creams, essences, nutritional essences, facial masks, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, oils, pressed powders, loose powders, and eye shadows.
The present invention will be described in detail below based on the following examples, which are intended to illustrate the present invention and not to limit the scope of the present invention.
Example 1 preparation of hydrangea macrophylla extract
The leaves of the hydrangea macrophylla are put into a dryer to be completely dried. The resulting mixture was immersed in 100% methanol or 70% ethanol aqueous solution to 3 times the dry weight. The solvent was removed by distillation under reduced pressure to obtain an extract of hydrangea macrophylla.
Example 2 analysis of Meadowrue extract
Detecting hydrangea macrophylla extract solution with concentration of 10ug/mL in methanol solvent at UV of 245nm, at 40 deg.C and 1.0mL/min and 100kgf/cm2HPLC equipped with a column under the conditions was injected with 10 ul. To add 0.1% TFA MeOH and 0.1% TFA H2O as mobile phase from H2O60%, MeOH 40% to 2: after the 98-point ratio, the conditions were returned to the initial conditions again, and the analysis was carried out for 60 minutes, the results of which are shown in FIG. 1.
Example 3 preparation of Mixed extract of hydrangea macrophylla and Citrus reticulata
And (3) adding the following components in percentage by weight of 6: mixing the dried leaves of the hydrangea macrophylla and the dried tangerine peel at a weight ratio of 4. The resulting mixture was immersed in 100% methanol or 70% ethanol aqueous solution to 3 times the dry weight. The mixed extract of hydrangea macrophylla and citrus peel is prepared by removing the solvent by distillation under reduced pressure.
Comparative example 1 preparation of hydrangea extract
The leaves of hydrangea are put into a dryer to be completely dried. The resulting mixture was immersed in 100% methanol or 70% ethanol aqueous solution to 3 times the dry weight. The solvent was removed by distillation under reduced pressure to obtain an extract of hydrangea.
Detecting the extract solution with concentration of 10ug/mL in methanol solvent with UV at 245nm, at 40 deg.C and 1.0mL/min and 100kgf/cm2HPLC equipped with a column under the conditions was injected with 10 ul. To add 0.1% TFA MeOH and 0.1% TFAH to the reaction mixture2O as mobile phase from H2O90%, MeOH 10% to 2: after the ratio of 98, the conditions of the initial conditions were returned again, analyzed for 45 minutes, and the results of the extract of the present invention from the hydrangea macrophylla under the same conditions are shown in FIG. 2 (upper graph: hydrangea macrophylla, lower graph: hydrangea macrophylla).
Experimental example 1 MMP-1 expression inhibition experiment of hydrangea macrophylla extract
The Hs68 cell line (Human Foreskin Fibroblast cell line; Human Foreskin Fibroblast cell line) was cultured under the following conditions. DMEM medium (Lonza #12-604F) was used as a culture solution after 10% FBS (Gibco) #16000- & 044) and antibiotics (1% P/S) were put therein. At 37 deg.C, 5% CO2The cells are cultured under conditions.
Hs68 cells were added to each well (well) of a 6-well plate (well plate) at 1.8X105Cells/2mL (cells/2mL), cultured for 24 hours. After the medium was changed to Serum-free (Serum-free) medium, the medium was cultured for another 6 hours, and then UVB was applied at 20mJ/cm2After stimulating and treating hydrangeal phenol (hydrangenol) (hydrangea macrophylla extract) and a positive control (retinoic acid, 1 μ M) (n ═ 3) at the concentrations, respectively, for 48 hours, the culture solution was collected and used for ELISA (R ═ 3)&D # DY901) method to determine MMP-1 amount. To correct for differences in Cell numbers between groups, MMP-1ELISA results were normalized (normalization) to CCK-8 assay (Cell Counting Kit-8assay, described below) results.
Cell proliferation & toxicity assay (CCK-8)
At the end of the MMP-1 assay, Hs68 cells were replaced with cells containing 5% CCK-8After culturing the solution (Dojindo) # CK04), the culture was incubated at 37 ℃ with 5% CO2The incubator was further incubated for 2 hours. Finally, the presence or absence of cell proliferation or toxicity was calculated by measuring the absorbance at a wavelength of 450nm and comparing the absorbance with that of a control group.
The results of MMP-1 expression inhibition experiments are shown in FIG. 3, and it is clear from FIG. 3 that the hydrangea macrophylla extract of the present invention exhibits an excellent MMP-1 protein expression inhibition effect.
Experimental example 2 moisturizing Gene expression experiment of hydrangea macrophylla extract
HEKn cells (Human Epidermal Keratinocytes, neonatal cells) were plated at 2X105One was inoculated (seeding) on a 6-well plate (well plate) and cultured for 24 hours. Samples were treated with hydrangea macrophylla extract at concentrations of 12ppm, 25ppm, and 50ppm, respectively, and after 24 hours, cells were lysed (lysis) using Trizol and RNA was isolated. After RNA quantification, cDNA was synthesized, and then real-time fluorescence quantitative PCR (real time PCR) was performed on each gene using a Tackmann probe (Taqman probe). As an Internal control (Internal control) gene, GAPDH gene was used. Finally, the relative gene expression values were quantified using the Ct values, and the results are shown in FIG. 4.
(example 3 and comparative example 2) preparation of cosmetic composition
Preparation method of cosmetic water containing extract of hydrangea macrophylla and pericarpium Citri Tangerinae
(Table 1)
| Composition (I) | Content (wt.) |
| Purified water | Balance of |
| Dipropylene glycol | 2.00 |
| EXAMPLE 3 hydrangea macrophylla/Citrus reticulata Mixed extract | 5.00 |
| Ethanol | 7.00 |
| Hydrogenated castor oil | 0.40 |
| Preservative | Proper amount of |
| Incense stick | Proper amount of |
Preparation method of oil containing hydrangea macrophylla and pericarpium Citri Tangerinae extract
(Table 2)
Preparation method of nourishing cream containing mixed extract of hydrangea macrophylla and pericarpium Citri Tangerinae
(Table 3)
(Experimental example 3) the cosmetic compositions of example 3 and comparative example 2 had the wrinkle-improving and moisturizing sensory effects
Sensory evaluation was performed using the nutritional creams prepared in the example 3 and the comparative example 2.
100 healthy women aged twenty to sixty years old were divided into two groups, one group was applied twice daily with the nourishing cream prepared in example 3 of the present invention, and the other group was applied twice daily with the nourishing cream prepared in comparative example 2. The evaluation was classified as very good, general, and unsatisfactory for feedback, and the results are shown in table 4 below.
(Table 4)
As can be seen from table 4, the cosmetic extract containing the hydrangea macrophylla extract of the present invention is very effective in improving wrinkles.
Claims (10)
1. A cosmetic composition for skin anti-aging and moisturizing, the cosmetic composition comprising hydrangea macrophylla extract.
2. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the hydrangea macrophylla extract contains hydrangeal phenol in an area ratio of 8% or more based on high performance liquid chromatography.
3. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the hydrangea macrophylla extract has a high performance liquid chromatography analysis result satisfying the following formula 1:
0.1＜I2/I1＜0.3(1)
in the case of the above-mentioned formula 1,
I2is prepared by detecting crude hydrangea in methanol at a concentration of 10ug/mL by UV at 245nm, at 40 deg.C and a concentration of 1.0mL/min and a concentration of 100kgf/cm2HPLC with a column installed under conditions that the HPLC conditions are 0.1% TFA H, the area value of the maximum peak that appears at a retention time between 24 minutes and 27 minutes when separation is performed2O：0.1％TFA MeOH＝40：60，
I1Is in the following general formula I2The area value of the maximum peak that occurs under the same conditions at retention times between 34 and 36 minutes.
4. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the cosmetic composition further comprises a dried orange peel extract.
5. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 4, wherein,
the hydrangea macrophylla and dried orange peel mixed extract is contained in an amount of 0.1 to 20 wt% with respect to the total weight of the composition.
6. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 4, wherein,
the mixed extract of the hydrangea macrophylla and the dried orange peel is prepared by mixing the following components in a weight ratio of 3: 7 to 7: 3 weight ratio of the extract containing hydrangea macrophylla and pericarpium Citri Reticulatae.
7. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the hydrangea macrophylla extract is obtained by extracting with C1-C4 alcohol or a mixture of C1-C4 alcohol and water.
8. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the cosmetic composition further comprises one or more moisturizers selected from glycerin, butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, and propanol.
9. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the cosmetic composition further comprises one or more selected from the group consisting of a fatty substance, an organic solvent, a dissolving agent, a concentrating agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic emulsifier, a filler, a metal ion-blocking agent, a chelating agent, a preservative, a vitamin, a blocking agent, a humectant, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, and a lipid vesicle.
10. The cosmetic composition for skin anti-aging and moisturizing as claimed in claim 1, wherein,
the cosmetic material composition is used for cosmetics selected from the group consisting of skin lotion, skin softener, toner, astringent, milky lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, essence, nourishing essence, mask, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, oil, pack, loose powder, and eye shadow.
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| KR10-2017-0146887 | 2017-11-06 | ||
| KR1020170146887A KR102152584B1 (en) | 2017-11-06 | 2017-11-06 | cosmetic composition for skin aging and moisturizing including extract of Jejudo hydrangea |
| PCT/KR2018/013151 WO2019088720A1 (en) | 2017-11-06 | 2018-11-01 | Cosmetic composition comprising hydrangea serrata extract for anti-skin aging and moisturization |
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| CN112603881A (en) * | 2020-12-23 | 2021-04-06 | 黄景添 | High-permeability moisturizing essence and preparation method thereof |
| CN114146032A (en) * | 2021-12-02 | 2022-03-08 | 科丝美诗(中国)化妆品有限公司 | Composition containing hydrangea macrophylla leaf extract and preparation method and application thereof |
| CN114272170A (en) * | 2021-11-23 | 2022-04-05 | 河南省鸣利隆医药科技有限公司 | Skin care product with blue light resisting effect and preparation method thereof |
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| KR102113563B1 (en) * | 2018-07-11 | 2020-05-29 | 코스맥스바이오 주식회사 | Composition for prevention and treatment of muscle disease, improvement of muscle function or enhancement of exercise performance comprising hydrangenol |
| KR20220156309A (en) | 2021-05-18 | 2022-11-25 | (주)아모레퍼시픽 | Composition for enhancing skin elasticity or improving skin wrinkles |
| KR20220166069A (en) | 2021-06-09 | 2022-12-16 | (주)아모레퍼시픽 | Composition for enhancing skin elasticity or improving skin wrinkles |
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| US20050013790A1 (en) * | 1999-05-10 | 2005-01-20 | Kao Corporation | External skin care composition |
| KR20090081178A (en) * | 2008-01-23 | 2009-07-28 | 주식회사 사임당화장품 | Cosmetic composition containing wild flower extracts |
| CN101522161A (en) * | 2006-10-13 | 2009-09-02 | 昭和电工株式会社 | Skin agent for external use and cosmetic agent including ubiquinone derivative or salt thereof and method using the same |
| CN103987385A (en) * | 2011-12-16 | 2014-08-13 | 株式会社爱茉莉太平洋 | Composition containing tangeretin for external application to the skin |
| KR20160082138A (en) * | 2014-12-31 | 2016-07-08 | (주)아모레퍼시픽 | Composition for improving skin condition comprising extract of hydrangea serrata |
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|---|---|---|---|---|
| KR101718854B1 (en) * | 2015-04-09 | 2017-03-23 | 충남대학교산학협력단 | Cosmetic composition containing Ligustrum lucidum Aiton, Astragalus membranaceus Bunge, Citrus unshin Markovich, Angelica dahurica root extract for moisturizing the skin |
| KR20160128765A (en) | 2015-04-29 | 2016-11-08 | 주식회사 엘지생활건강 | Composition for improving skin |
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2017
- 2017-11-06 KR KR1020170146887A patent/KR102152584B1/en active IP Right Grant
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- 2018-11-01 CN CN201880070927.9A patent/CN111315354A/en active Pending
- 2018-11-01 WO PCT/KR2018/013151 patent/WO2019088720A1/en active Application Filing
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| US20050013790A1 (en) * | 1999-05-10 | 2005-01-20 | Kao Corporation | External skin care composition |
| CN101522161A (en) * | 2006-10-13 | 2009-09-02 | 昭和电工株式会社 | Skin agent for external use and cosmetic agent including ubiquinone derivative or salt thereof and method using the same |
| KR20090081178A (en) * | 2008-01-23 | 2009-07-28 | 주식회사 사임당화장품 | Cosmetic composition containing wild flower extracts |
| CN103987385A (en) * | 2011-12-16 | 2014-08-13 | 株式会社爱茉莉太平洋 | Composition containing tangeretin for external application to the skin |
| KR20160082138A (en) * | 2014-12-31 | 2016-07-08 | (주)아모레퍼시픽 | Composition for improving skin condition comprising extract of hydrangea serrata |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112603881A (en) * | 2020-12-23 | 2021-04-06 | 黄景添 | High-permeability moisturizing essence and preparation method thereof |
| CN114272170A (en) * | 2021-11-23 | 2022-04-05 | 河南省鸣利隆医药科技有限公司 | Skin care product with blue light resisting effect and preparation method thereof |
| CN114146032A (en) * | 2021-12-02 | 2022-03-08 | 科丝美诗(中国)化妆品有限公司 | Composition containing hydrangea macrophylla leaf extract and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019088720A1 (en) | 2019-05-09 |
| KR102152584B1 (en) | 2020-09-08 |
| KR20190051350A (en) | 2019-05-15 |
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