KR20180097101A - A cosmetic composition for anti-aging comprising extract of coffee grounds - Google Patents

Abstract

The present invention relates to an anti-aging cosmetic composition. More specifically, provided is a cosmetic composition containing a coffee waste extract as an active ingredient. According to the present invention, the anti-aging cosmetic composition ensures excellent skin wrinkle amelioration effects and antioxidant effects.

Description

TECHNICAL FIELD The present invention relates to an anti-aging cosmetic composition containing an extract of coffee bean as an active ingredient,

The present invention relates to a cosmetic composition containing an extract of coffee bean as an active ingredient.

Skin is the primary barrier of the human body. It protects the internal organs of the body from external stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control.

As the industry develops, environmental pollution becomes serious, and there is a growing interest in skin aging prevention and whitening. In particular, human skin constantly undergoes changes, the most representative of which is degradation of the skin caused by aging and a reduction in visual beauty. The final appearance of skin appearance as a result of skin aging is wrinkle formation, skin elasticity decrease, senile spot formation, and wrinkle formation is considered to be the most typical phenomenon.

Lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, can be oxidized to destroy skin cells and tissues, and the skin may eventually age. Particularly, oxidation of proteins can cause collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, etc., which are connective tissues of the skin, to be cleaved and hyperinflammation reaction. In the case of further deepening, mutation due to DNA mutation, Cancer induction can be promoted.

Therefore, it is necessary to protect the cell membrane by eradicating reactive oxygen species which are mediated by reactive oxygen species, ultraviolet ray irradiation, and inflammation reaction occurring during the metabolism of the body, regenerate damaged cells through active metabolism, and restore skin health desirable.

Skin aging involves an enzyme called MMP (matrix metalloproteinase) as well as the active oxygen species. Although the synthesis and degradation of extracellular matrix such as collagen in vivo is appropriately controlled, as the aging process progresses, the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And wrinkles are formed.

In addition, such a degrading enzyme can be further activated by ultraviolet rays, and it is necessary to develop a substance capable of regulating the activation of MMP induced by ultraviolet light or inhibiting its activity. However, the raw materials conventionally used as cosmetic materials merely suppressed the activity of the degrading enzyme.

The cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

On the other hand, the coffee residue, which is extracted from coffee beans, is a large amount of wastes generated by the world over 6 million tons per year. However, there is a lack of utilization and treatment methods. Therefore, there is a growing interest in the recycling of coffee beans, and research has been conducted on the use of coffee beans as fuel, feed, or fertilizer additives, or as substrates for producing mushrooms and as raw materials for biodiesel.

The inventors of the present invention have studied the possibility and application of the coffee bean closely and found that the content of the effective ingredient showing the skin improvement effect can be significantly increased by optimizing the extraction conditions and utilized the material as a functional cosmetic material.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a natural cosmetic composition having not only superior functionality but also superior biostability.

According to one aspect of the present invention, there is provided an antioxidant cosmetic composition comprising an extract of coffee bean as an active ingredient.

In one embodiment, the extract may be extracted with ethanol at a concentration of 40 to 60% (w / w).

In one embodiment, the extract may be extracted at 90-110 < 0 > C.

According to another aspect of the present invention, there is provided a cosmetic composition for improving wrinkles comprising a coffee ground extract as an active ingredient.

In one embodiment, the extract may be extracted with ethanol at a concentration of 90 to 100% (w / w).

In one embodiment, the extract may be extracted at 45-65 < 0 > C.

According to another aspect of the present invention, there is provided an anti-aging cosmetic composition comprising a coffee ground extract as an active ingredient.

In one embodiment, the cosmetic composition is comprised of a softening agent, a nutritional lotion, a water cream, a nutritional cream, a massage cream, an essence, an ampoule, a gel, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, Lt; / RTI > may be formulated into one or more selected from the group.

Since the cosmetic composition according to one aspect of the present invention contains an active ingredient of a natural raw material, it is not only beneficial to skin health but also has excellent skin wrinkle improving effect and antioxidative effect.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

FIG. 1 is a view for optimizing polyphenol extraction conditions of a coffee grounds extract through a reaction surface method.
FIG. 2 is a view for optimizing conditions for extracting collagenase inhibitor from coffee bean extract through a reaction surface method.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided an antioxidant cosmetic composition comprising an extract of coffee bean as an active ingredient.

The coffee grounds are extracted residues produced in the process of producing coffee hot water extracts, which are estimated to produce over 6 million tons / year worldwide. (Saenger, M., Hartge, E. Werther, J. Ogada, T. and Siagi, Z. 2001. Combustion of coffee husks. Renew. Energ. 23: 103- 121.), a functional material source (Simoes, J., Madureira, P., Nunes, FM, Domingues, MR Vilanova, M. and Coimbra, MA 2009. Immunostimulatory properties of coffee mannans. : 1036-1043.), A biodiesel feedstock (Franca, AS, Oliveira, LS and Ferreira, ME 2009. Kinetics and equilibrium studies of methylene blue adsorption by spent coffee grounds. Desalination 249: 267-272. ought.

As used herein, the phrase " comprising as an active ingredient " may mean an effective amount of the composition capable of exhibiting a skin improving effect, for example, exhibiting collagen synthesis, elasticity improvement or antioxidative effect related to a wrinkle reducing effect.

The coffee bean contains various physiologically active ingredients. In particular, the present inventors have maximized the skin wrinkle and antioxidant activity by optimizing the coffee bean extraction condition and utilized it as a material for a cosmetic composition.

The above-mentioned "extract" refers to a solvent in which an effective ingredient contained in an extraction raw material has been transferred by contacting a solvent and an extraction raw material under specific conditions. If a substance is separated from a natural product, the extraction method, You can include them all regardless. For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil.

The extract may be obtained by drying the coffee bean after the coffee bean has been pulverized, or by various extraction methods known in the art such as a hot water extraction method and an ethanol extraction method.

Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol , And may be extracted with ethanol, preferably.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

The extract is washed with water, dried and pulverized, and extracted and extracted with a solvent having a volume of 8 to 12 times the weight of the raw material for about 1 to 24 hours by a conventional method such as circulation extraction, pressure extraction, Followed by filtration. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

In particular, the extract can be extracted at a temperature of 90 to 110 ° C by ethanol at a concentration of 40 to 60% (w / w), and the extraction efficiency of polyphenol can be maximized and the antioxidant activity can be increased under the above conditions.

According to another aspect of the present invention, there is provided a cosmetic composition for improving wrinkles or improving elasticity, which comprises coffee bean extract as an active ingredient. In addition, the coffee ground extract has an excellent antioxidative effect and wrinkle-reducing effect, and thus can be utilized as a material for an anti-aging cosmetic.

The extract can be extracted at a temperature of 45 to 65 ° C by ethanol at a concentration of 90 to 100% (w / w), and the collagenase inhibitory activity is maximized under the above conditions, thereby improving the skin wrinkles and elasticity The effect can be increased.

The "wrinkles " refers to the scarring caused by degeneration of the skin, and may be caused by a cause of genes, a reduction of collagen and elastin present in the skin dermis, and an external environment.

The "wrinkle improvement" refers to a phenomenon that inhibits or inhibits the generation of wrinkles on the skin, or alleviates the already generated wrinkles.

The "skin elasticity" means elasticity realized by elastic fibers composed of elastin existing in the dermal layer. Since the elastic fibers have a low elastic modulus like rubber, they are easily deformed by external force, It can be easily restored to its original state.

The cosmetic composition may be at least one selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Can be formulated.

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. The cosmetic composition may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Production Example 1: Preparation of Coffee Bean Extract

Roasted coffee bean flour was purchased and used for the experiment. The water-soluble component was removed by extraction with water at 90 ° C for 30 seconds using a coffee maker, and the coffee bean powder in which the water-soluble component was removed was dried to remove moisture to prepare a coffee bean.

Ethanol concentration was used as extraction solvent and ultrasonic extraction method was used for low temperature extraction.

A closed glass tube was mixed with 2 to 40 times volume of ethanol to the coffee ground powder sample and extracted at 20 to 100 DEG C for 5 to 60 minutes.

The samples were extracted under stand-bath conditions using ultrasonic waves (40 kHz, 200 W) at room temperature. During extraction, the tube was placed in a stainless rack so that the bottom of the tube did not reach the bottom of the ultrasonic bath, and the extract and solvent in the container were immersed in water.

1 mL of the extract was transferred to a microtube and centrifuged. The supernatant was recovered, and a diluent was prepared as needed. Total polyphenol (TPC) and collagenase inhibitory activity were measured.

Production Example 2: Optimization of Polyphenol Extraction Conditions of Coffee Bean Extract

The concentration of polyphenol produced by ultrasonic extraction was compared with that of other extraction parameters such as temperature, time and ethanol concentration.

The maximum polyphenol value of 15.9 mg GAE / g DW was calculated at the extraction temperature of 100.0 ℃, the extraction time of 31.9 sec, and the ethanol content of 54.6% (w / w) 1).

Production Example 3: Optimization of extraction conditions of collagenase inhibitor of coffee ground extract

In order to clarify the relationship between the concentration of physiologically active substance (collagenase inhibitor) produced by ultrasonic extraction and each extraction parameter, the collagenase inhibitory activity was compared by varying the temperature, time and ethanol concentration as the main extraction parameters.

As a control, 0.0002 mg / ml retinol was used to inhibit collagenase. When the optimum conditions for collagenase inhibition activity were optimized using the reaction surface method, the maximum collagenase inhibition rate was calculated to be 182.7% at an extraction temperature of 55.1 ° C, an extraction time of 60 seconds, and ethanol of 100.0% ).

Examples and Comparative Examples

To examine the anti-aging effect of the coffee ground extract, examples and comparative examples were set up in different extraction conditions as shown in Table 1 below.

division Ethanol concentration (w / w) Extraction temperature (캜) Extraction time (seconds) Example 1 55 110 32 Example 2 40 110 32 Example 3 60 110 32 Example 4 55 90 32 Example 5 40 90 32 Example 6 60 90 32 Example 7 55 100 32 Example 8 100 55 60 Example 9 100 45 60 Example 10 100 65 60 Example 11 90 55 60 Example 12 90 45 60 Example 13 90 65 60 Comparative Example 1 70 100 32 Comparative Example 2 30 100 32 Comparative Example 3 55 80 32 Comparative Example 4 55 120 32 Comparative Example 5 80 55 60 Comparative Example 6 70 55 60 Comparative Example 7 100 35 60 Comparative Example 8 100 75 60

Experimental Example 1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

The samples of Examples and Comparative Examples were each suspended in purified water at a concentration (50 mg / L), and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added to each well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example 2: Measurement of polyphenol content

The content of polyphenols contained in the fermented extracts of Examples and Comparative Examples was measured. Polyphenol has excellent radical scavenging activity and antioxidant activity.

Quantification of polyphenol was performed according to AOAC. 1 mL of 1N-Folin-Ciocalteu phenol reagent reagent was added to 1 mL of the sample solution diluted 100 times with the samples of Examples and Comparative Examples. Add 0.2 mL of 1N HCl, add 10 mL of saturated Na ₂ CO ₃ solution (2% v / v), mix and react at room temperature for 30 minutes. After measuring the absorbance at 750 nm, the content of polyphenol was calculated by comparing the absorbance of the standard curve with a standard curve prepared with galic acid.

The contents of polyphenols contained in the samples of Examples and Comparative Examples were measured and the results are shown in Table 2 below.

division Polyphenol content (mg GAE / g DW) Example 1 15.2 Example 2 14.3 Example 3 14.6 Example 4 15.0 Example 5 14.1 Example 6 14.5 Example 7 15.9 Comparative Example 1 13.1 Comparative Example 2 12.2 Comparative Example 3 13.2 Comparative Example 4 11.9

The polyphenol contents of the samples of Examples 1 to 7 were evaluated to be significantly higher than those of Comparative Examples 1 to 4. Particularly, it was confirmed that the polyphenol content of the sample of Example 7 in which 55.0% (w / w) of ethanol was used as a solvent and the extraction temperature and extraction time were controlled at 100 ° C for 32 seconds was the highest.

The above results suggest that the antioxidative activity of the coffee ground extract can be significantly increased by controlling the extraction conditions to a specific range.

Experimental Example 3: Collagenase inhibitory activity test

The collagenase inhibitory activity was measured in vitro to compare the wrinkle-reducing effects of the examples and the comparative examples.

Since the degradation of collagen is increased and wrinkles are formed due to the expression of MMP (Matrix Metalloproteinase) such as collagenase by UV which is the cause of photoaging, collagenase inhibitory activity is experimented to show the effect of inhibiting skin aging Can be confirmed.

4 mM CaCl ₂ was added to a 1 M Tris-HCl buffer (pH 7.5) and 4-phenylazovenzyloxycarbonyl-Pro-Leu-Gly-Pro- Arg, 0.3 mg / mL), and 150 μL of collagenase (0.2 mg / mL) was added to the mixture of 100 μL of coffee bean extract.

After incubation at room temperature for 20 minutes, 500 μL of 6% citric acid was added to stop the reaction. After 1.5 mL of ethyl acetate was added, the supernatant was taken and the absorbance at 320 nm was measured to calculate the inhibitory activity of collagenase. The evaluation results are shown in Table 3 below.

division Collagenase inhibitory activity (%) Example 8 182.7 Example 9 175.9 Example 10 176.4 Example 11 176.3 Example 12 172.7 Example 13 173.4 Comparative Example 5 162.4 Comparative Example 6 156.7 Comparative Example 7 159.1 Comparative Example 8 153.6

The samples of Examples 8 to 13 effectively inhibited the activity of collagenase as compared with those of Comparative Examples 5 to 8. Particularly, it was confirmed that the collagenase inhibitory activity of Example 8 in which 100.0% (w / w) of ethanol was used as a solvent and the extraction temperature and extraction time were controlled to 55 ° C and 60 seconds was the highest, The collagenase inhibitory activities of Comparative Examples 5 to 8 were evaluated to be significantly low.

The above results suggest that the collagenase inhibitory activity of the coffee ground extract can be significantly increased by controlling the extraction conditions to a specific range.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

A cosmetic composition for antioxidation comprising an extract of coffee ground as an active ingredient. The method according to claim 1,
Wherein the extract is extracted with ethanol at a concentration of 40 to 60% (w / w). 3. The method of claim 2,
Wherein the extract is extracted at 90 to 110 캜. A cosmetic composition for improving wrinkles comprising an extract of coffee bean as an active ingredient. 5. The method of claim 4,
Wherein the extract is extracted with ethanol at a concentration of 90 to 100% (w / w). 6. The method of claim 5,
Wherein the extract is extracted at 45 to 65 占 폚. A cosmetic composition for anti-aging comprising coffee bean extract as an active ingredient. 8. The method according to any one of claims 1 to 7,
Cosmetics formulated with at least one selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, essence, ampule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Composition.

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