KR20210041220A - A cosmetic composition comprising microalgae-derived peptide - Google Patents

Abstract

The present invention relates to a cosmetic composition, and provides a cosmetic composition having excellent skin whitening and skin regeneration activity and excellent cell stability by including a microalgae-derived peptide.

Description

Cosmetic composition containing microalgae-derived peptide {A COSMETIC COMPOSITION COMPRISING MICROALGAE-DERIVED PEPTIDE}

The present invention relates to a cosmetic composition comprising a microalgal-derived peptide to enhance skin whitening and skin regeneration activity.

The skin is the most basic defense organ that protects the body against external stimuli by contacting the external environment, and is an important organ expressing external beauty. Skin is not only a criterion for judging that external beauty symbolizes youth and health, but also has established itself as a competitive edge that affects work ability by expressing confidence.

However, as the amount of external activity of people increases, the skin is damaged by ultraviolet rays as the skin is exposed to sunlight for a long time. When the skin is exposed to ultraviolet rays for a long time, skin aging proceeds, and the skin becomes black due to the melanin pigment generated to protect the inner layer of the skin from ultraviolet rays.

Therefore, the consumption of functional cosmetics is increasing as a way to manage the skin in the modern society, and because white, clean, young and elastic skin like white jade is preferred, the cosmetic industry is actively developing the whitening effect and anti-aging.

The whitening effect of cosmetics refers to whitening the user's skin, and most whitening cosmetics have a function of suppressing the production of melanin. Melanin is produced by melanocytes present in the basal layer of the epidermis, and the activity and distribution of melanocytes determine human skin color.

Tyrosine, a type of amino acid, is converted into dopa (dihydroxy phenylalanine) by an enzyme called tyrosinase contained inside melanocytes, and dopaquinone and dopachrome, etc. Melanin, a polymer, is finally produced through a series of oxidation processes that go through. Therefore, most whitening cosmetics exhibit a whitening effect on the principle of inhibiting the production of melanin by inhibiting the activity of tyrosinase, a major enzyme in melanin production.

The anti-aging of cosmetics is to block or eliminate the causes that promote skin aging. The skin aging may appear naturally with age or may occur due to photoaging due to sunlight.

The photoaging is caused by an imbalance between oxidation and antioxidant due to the generation of excess reactive oxygen species by ultraviolet rays, and reduction of antioxidant enzymes and antioxidants such as vitamins E, C, glutathione and ubiquinol in the body.

As photoaging progresses, not only the reaction to produce melanin is accelerated, but also damage to biological components due to lipid peroxidation, protein oxidation, activation of proteases, chain cleavage and abnormal crosslinking of collagen and elastin, hyaluronic acid chain cleavage, DNA oxidation, etc. In the end, skin aging occurs, such as reduction of skin elasticity, wrinkles and spots, and freckles. Accordingly, a cosmetic composition having an antioxidant effect while inhibiting the activity of tyrosinase can effectively induce a skin whitening effect and prevent skin aging.

Korean Patent Publication No. 10-2018-0097101

An object of the present invention is to provide a cosmetic composition comprising a peptide derived from microalgae.

One aspect of the present invention for achieving the above object provides a cosmetic composition comprising a peptide derived from microalgae.

The microalgae may be skim microalgae.

The "microalgae" is a single-celled organism that grows photosynthetically using water, carbon dioxide and sunlight, and is also called phytoplankton. The microalgae may be cultured or harvested. The cultured microalgae may contain a culture medium, and the harvested microalgae may contain seawater or fresh water together depending on the habitat of the microalgae. The microalgae is not a taxonomic term for all photosynthetic organisms except terrestrial plants, and may be referred to as a general term referring to a wide variety of taxa. Among these, unicellular algae that can be observed under a microscope are called microalgae, and most phytoplankton belong to this.

In general, the microalgae grow much faster than terrestrial plants and have high productivity of viable organisms, that they grow easily in natural environments where light energy can be secured as well as fresh water or seawater, and proteins, lipids, and sugars in various algae. As well as biopolymer materials of industrial interest such as pigments, as well as materials having specific physiological functions can be produced at a high concentration, it has excellent applicability as an important bioindustrial material.

The "lipid-extracted microalgae" is a solid by-product remaining after extracting lipids after cultivation of microalgae, and accounts for 30 to 50% of the dry weight of the microalgae. As a mass cultivation of microalgae, commercial production of chlorella as a health supplement began in Asia in the 1960s, and in France, spirulina was started mass cultivation to develop it as a protein source. Spirulina has a very high protein content of 46 to 71% of dry weight and contains a large amount of physiologically active substances such as γacid (GLA), phycocyanin, oxanthophyll, and zeaxanthin, so it is preferred as an excellent material that provides protein or vitamins as animal feed as well as food supplement Has become.

In addition to proteins, the microalgae contains some carbohydrates and lignin, and has a form in which lignin, a combination of various phenolic substances, surrounds and protects the outside. Skim microalgae contains natural antioxidants including phenolic compounds, and contains a large amount of functional materials such as polysaccharides and antioxidants that are not eluted in the solvent used for lipid extraction. It's worth it.

The “peptide” is a compound formed by binding of two or more amino acids to an α-carboxyl group and an α-amino group in a chain or ring shape. The peptides are classified into dipeptide, tripeptide, tetrapeptide, oligopeptide or polypeptide according to the number of amino acid bonds. The number of oligopeptides (2 to 10 bound amino acids) or polypeptides (10 to 50 amino acids) is not necessarily determined.

The size of the peptide may be 1.4 kDa or less, and the number of amino acids of the peptide may be 13 or less.

When the size of the peptide is 1.4 kDa or less or the number of amino acids is 13 or less, the skin absorption rate may be increased because the molecule size is small, and the functionality may be improved when applied as a cosmetic product.

Pretreatment step of pulverizing the microalgae and freeze-drying the peptide derived from the microalgae; Peptization step of mixing the powdered microalgae and an enzyme solution containing a proteolytic enzyme; And a peptide separation step of filtering the mixture and obtaining a peptide by centrifugation.

In the pretreatment step of the preparation method, a step of pulverizing the microalgae and sedimentation in a solvent (acid/base/organic solvent) to selectively extract the protein may be further added. Due to the high protein content step, since only the protein in the microalgae can be selectively enzymatically decomposed, the efficiency of producing the peptide can be improved.

In the pretreatment step of the above manufacturing method, lyophilization is carried out by sublimation to make ice directly into steam by lowering the partial pressure of water wapor. When the residual moisture is maintained at 2 w/w% or less through the lyophilization, the pulverized material can be stably stored and transported without loss of useful substances.

In the peptization step of the manufacturing method, the proteolytic enzyme is an enzyme that hydrolyzes a protein, and is mixed with the crushed microalgae for a certain period of time to undergo enzymatic digestion.

The protease may be a food-derived strain, and the food may be cheonggukjang, doenjang, natto, soy sauce, and kimchi, but is not limited thereto.

The strain of Bacillus (Bacillus), Lactobacillus bacteria (Lactobacillus), Penny room Solarium (Penicillium), peusa Solarium (Fusarium), Pichia (Pichia), Candida (Candida), wrapped horizontally My process (Saccharomyces), Pseudomonas (Pseudomonas) , Citeromyces , and Aspergullus , enzymes derived from microorganisms may be used. In addition, Fusarium illudens, F. lateritium, F. solani, Paecilomyces sp., Penicillium aurantiogriseum Dierckx, P. brevicompactum, P. citrinum, P. chrysogenum, P. corylophilum, P. crustosum, P. expansum, P. fellutanum, P. implicatum, P. roqueforti, P. solitum and P. Waksman . Bacillus subtilis natto may be, but is not limited thereto.

In order to produce a protease in the strain, a liquid or solid enzyme production medium is used. In the medium for enzyme production, the strain is inoculated at 10 v/v%, cultured at 30 to 40° C. for 24 hours, centrifuged at 13,000 xg for 20 minutes, and then the supernatant can be recovered and used as a crude enzyme solution. .

The cosmetic composition may be for skin whitening or skin regeneration.

The above "whitening" alleviates skin pigmentation phenomena such as blemishes, freckles, and blemishes by inhibiting the production of various biomolecules such as melanin, oxidation-reduction hemoglobin, carotene, and melanoids, which affect the skin color. It refers to the effect of improving skin brightness and uniformity by relieving energy. In particular, it is possible to improve skin tone by inhibiting the activity of tyrosinase, which is a melanin pigment-generating enzyme.

The “skin regeneration” refers to an effect such as preventing and improving skin wrinkles, which prevents skin aging. Skin wrinkles are caused by the degradation of collagen, and skin wrinkles can be improved by inhibiting collagenase, which is a collagen-degrading enzyme.

In one embodiment of the present invention, it was confirmed that the skin safety of the functional peptide (Example 1) obtained by mixing a food-derived strain with microalgae was superior to that of using a commercial enzyme (Table 2), and tyrosinase inhibition By confirming that the activity (Table 3) and the collagenase inhibitory activity (Table 4) are also excellent, it was confirmed that the peptide derived from microalgae of the present invention can be usefully used as a cosmetic composition for skin whitening and skin regeneration.

The cosmetic composition is one or more selected from the group consisting of softening lotion, nutritional lotion, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. It can be formulated.

The cosmetic composition may be formulated by a conventional method. Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can refer to the contents disclosed in the formulation of external preparations for skin, and the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) in the formulation of cosmetic compositions. , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilization formulation. For example, a lotion such as a flexible lotion or a nutritional lotion; Emulsions such as facial lotion and body lotion; Creams such as nourishing cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; It may be formulated as a liquid type, solid type, or spray type foundation, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.

In each formulation, the cosmetic composition may be appropriately blended with other components within a range that does not impair the object according to the present invention depending on the type of formulation or purpose of use, in addition to the essential components in each formulation.

The cosmetic composition may contain a generally acceptable carrier, and for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or these Mixtures can be used.

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further include a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil can be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan can be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients. The adjuvant and its mixing ratio can be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

The cosmetic composition according to an aspect of the present invention contains a peptide derived from microalgae, which is a natural raw material, so that it is not only excellent in human stability, but also is beneficial to skin health, and has excellent whitening and skin regeneration effects.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a measurement of peptide molecular weights of Examples and Comparative Examples according to an embodiment of the present invention by SDS-PAGE. Specifically, FIGS. 1A and 1B are Comparative Examples 1 to 3, and FIGS. 1C and 1D are Examples. The molecular weights of the peptides of 1 and Comparative Examples 4 to 8 were measured.
FIG. 2 shows the results of MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) analysis of the peptide of Example 1 according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the present invention is not limited by the following examples.

Experimental Example 1: Selection of microalgae and analysis of components

1-1. Analysis of microalgae composition

The general components of microalgae were measured according to the AOAC standard analysis method.

As a result, based on the dried microalgae, it was composed of 10 to 40% crude protein, 20 to 40% crude fat, 10 to 40% crude, and 10 to 25% carbohydrates. In this case, it contains more than 70% protein.

The above results suggest that the microalgae contains sufficient protein to produce a functional peptide, and in particular, the microalgae can be more usefully utilized in the production of functional peptides by maximizing the protein content through a degreasing process.

1-2. Selection of microalgae

In order to produce a large amount of functional peptides, it is important to select microalgae with a high protein content.

Therefore, after performing the measurement of the general component of 1-1, Scensdesmus obliquus was selected as a microalgae having a high protein content.

Experimental Example 2: Preparation of microalgae-derived peptide

In order to produce functional peptides derived from microalgae or skim microalgae, the microalgae selected in 1-2 above are extracted with distilled water in hot water and then freeze-dried to obtain a solid (control), and then the protein of the solid is used as a strain derived from a traditional food. Thus, a peptide was obtained, or a peptide was obtained using chemical digestion or a commercial enzyme.

2-1. Preparation of functional peptides derived from microalgae using strains derived from traditional foods

Cheonggukjang, soybean paste, and soy sauce were used as the traditional food, and a strain ( B. subtilis natto ) having excellent protein degradability among the traditional foods was selected and used.

The strain was incubated in YP broth (yeast extract 1%, peptone 2%) at 37°C for 16 hours, then inoculated in 100 mL of enzyme production medium at 10 v/v% and incubated at 37°C for 24 hours. , After centrifugation (13,000 × g) for 20 minutes, the supernatant was recovered and used as a coenzyme solution for decomposing microalgae protein.

1 g of the solid and the crude enzyme solution were mixed at a ratio of 1:30 (w/v), and reacted in a shaking incubator (SIB-05RH, Jeongbio, Korea) at 50° C. 200 rpm for 8 hours, and then 95° C. water After heating for 10 minutes to inactivate the enzyme, centrifugation at 3,000 xg for 20 minutes to obtain a supernatant, the obtained supernatant was filtered to remove impurities, and freeze-dried to obtain a solid peptide sample (Example 1) Was obtained.

2-2. Preparation of functional peptides derived from microalgae through chemical decomposition

The chemical decomposition is performed through basic decomposition or acid decomposition, and the basic decomposition is a mixture of 1 g of the solid and 1 mol NaOH in a ratio of 1:30 (w/v), and a shaking incubator (SIB-05RH, Jeongbio, Korea) After reacting at 50° C. at 200 rpm for 8 hours, heating the enzyme at 95° C. water for 10 minutes to inactivate the enzyme, and centrifuging at a rate of 3,000 xg for 20 minutes to obtain a supernatant, and the obtained supernatant was filtered. Impurities were removed and lyophilized to obtain a solid peptide sample (Comparative Example 1).

The acid decomposition was performed using 1 mol H ₂ SO ₄ (Comparative Example 2) or 6 mol HCl (Comparative Example 3) instead of the 1 mol NaOH to obtain a peptide sample.

2-3. Preparation of functional peptides derived from microalgae using commercial enzymes

The method of obtaining a peptide using the commercial enzyme is sodium phosphate buffer (SPB, Comparative Example 4), Alcalase (Comparative Example 5), Protamex (Comparative Example 6), and Nutra in 1 g of the solid. It was obtained by mixing the agent (Neutrase, Comparative Example 7) or Flavourzyme (Comparative Example 8).

Specifically, the SPB was mixed with 0.2 mol, Alkarease, Protamex, Neutrase, and Flavorzyme were diluted and mixed in a ratio of 1:300 (w/v), which is a commonly used dilution factor, and a shaking incubator (SIB-05RH, Jeongbio, Korea) reacted for 8 hours at 50° C. 200 rpm for 8 hours, heated in 95° C. water for 10 minutes to inactivate the enzyme, and then centrifuged at 3,000 xg for 20 minutes to obtain a supernatant. Then, the obtained supernatant was filtered to remove impurities, and lyophilized to obtain a solid peptide sample.

Table 1 below shows the results of comparing the proteolytic activity of the crude enzyme solution used in Example 1 with the alkalase and distilled water used in Comparative Example 4.

division Protease activity (unit/mL) Example 1 708.5 Comparative Example 4 355.1 Distilled water 0

The above results suggest that the proteolytic enzyme activity in the strain derived from traditional food is sufficient compared to the standard for general use of conventional commercial enzymes.

Experimental Example 3. Peptide molecular weight measurement

SDS-PAGE was used to measure the molecular weight of the peptides contained in the samples of the control, examples and comparative examples.

For SDS-PAGE, a gel consisting of 12-16.5% separating gel and 5% stacking gel was used.

The staining buffer was used by mixing 0.1% Coomassie brilliant blue R-250, 45% methanol and 10% acetic acid, and the de-staining buffer was used by mixing 10% acetic acid and 45% methanol.

Standard molecular weight markers were SDS-PAGE molecular weight standards (Biorad, USA) and polypeptide SDS-PAGE molecular weight standards.

After electrophoresis using an electrophoresis device, the gel was stained for at least 2 hours using a staining buffer, and then bleached with a destaining buffer.

The peptide molecular weight measurement results are shown in FIG. 1.

Referring to FIGS. 1A and 1B comparing the molecular weight of the peptide by chemical decomposition with the control group, bands were identified in the 3.4, 5.5 and 6.5 kDa subgroups in the case of the control group. In Comparative Example 1, a drag phenomenon occurred due to base decomposition, and all peptides were present at 1.4 kDa or less.

In the case of Comparative Examples 2 and 3, a band between 1.4 and 200 kDa was not identified, and was decomposed into about 13 or less amino acids less than 1.4 kDa.

Referring to Figures 1c and 1d comparing the molecular weight of peptides degraded with commercial enzymes or food-derived strains, bands appeared in Comparative Examples 4, 6 and 7 at 16 to 26.6 kDa, confirming that the protein was not degraded.

However, in Example 1 and Comparative Examples 5 and 8, no band appeared at 16 to 26.6 kDa. This suggests that when the protein of microalgae is decomposed into food-derived strains, alkarazes or protamex, the peptides are degraded to 1.4 kDa or less, and food-derived strains can be utilized by replacing commercial enzymes.

MALDI-TOF-MSMS (matrix-assisted laser desorption/ionization time off light mass spectrometry) was used to confirm the more accurate molecular weight of Example 1 in which the functional peptide was prepared through a food-derived strain (FIG. 2).

Referring to FIG. 2, the peptide of Example 1 was present between 1.2 to 1.4 kDa, which suggests that the food-derived strain can decompose microalgal proteins into 13 or less functional peptides.

Experimental Example 4: Tyrosinase inhibitory activity experiment

In order to measure the tyrosinase inhibition activity of the samples of Examples and Comparative Examples, the DOPA Oxidase method was modified and used.

Tyrosinase is an enzyme that reacts with tyrosine to form melanin, and when tyrosinase is inhibited, melanin production is inhibited, so a whitening effect appears.

Buffer is 125 unit/mL mushroom tyrosinase (Sigma-Aldrich Co, Louis, MO) in a mixture of 0.4 mL of 67 mmol sodium phosphate buffer (pH 6.8) and 0.2 mL of 10 mmol 3,4-dihydroxy phenylanin (L-dopa) solution. , USA) 0.1 mL and 0.2 mL of a sample were added.

The reaction mixture was reacted at 37° C. for 20 minutes, and the amount of DOPAchrome produced in the reaction solution was measured at a wavelength of 475 nm.

As a control, distilled water was mixed instead of the sample.

The tyrosinase inhibitory activity was calculated as the ratio with the sample non-added group according to the following Equation 1 (Table 3).

[Equation 1]

Tyrosinase inhibitory activity (%) = 100 x (1-(absorbance of each sample added group / absorbance of non-sample added group))

division Tyrosinase inhibitory activity (%) Example 1 22.1 Comparative Example 1 7.4 Comparative Example 2 10.2 Comparative Example 3 10.5 Comparative Example 4 0.0 Comparative Example 5 20.7 Comparative Example 6 15.1 Comparative Example 7 13.5 Comparative Example 8 12.8 Control 1.3

Referring to Table 2, Example 1 (22.1%) using a strain derived from a traditional food was superior to tyrosinase inhibitory activity than the comparative example, and in particular, Comparative Examples 5 and 8 showing similar molecular weights of peptides (20.7 and 12.8%) showed excellent tyrosinase inhibitory activity.

Experimental Example 5: Collagenase inhibitory activity test

The samples of Examples and Comparative Examples were evaluated for collagenase inhibition activity.

Substrate solution 125 in which 0.3 mg/mL of 4-phenylazobenzyl oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (sigma, USA) was dissolved by adding _{4 mM CaCl 2} to 0.1 M tris-HCl buffer (pH 7.5). 50 μL of the sample was mixed in μL.

0.075 mL of 1 mg/mL collagenase from Clostridium histolyticum (Sigma-Aldrich Co, Louis, MO, USA) was added to the mixture.

As a control, distilled water was mixed instead of the sample.

After allowing to stand at room temperature for 20 minutes, 0.5 mL of 6% citric acid was added to stop the reaction, and 2 mL of ethyl acetate was added to measure the absorbance at 320 nm with a spectrophotometer (UV-1650PC, Shimadzu, Kyoto, Japan).

The collagenase inhibitory activity was calculated as the ratio with the sample non-added group according to Equation 3 below (Table 3).

[Equation 2]

Collagenase inhibitory activity (%) = 100 x (1-(absorbance of each sample added group / absorbance of non-sample added group))

division Collagenase inhibitory activity (%) Example 1 57.5 Comparative Example 1 10.7 Comparative Example 2 54.0 Comparative Example 3 30.6 Comparative Example 4 22.3 Comparative Example 5 48.0 Comparative Example 6 42.8 Comparative Example 7 41.4 Comparative Example 8 37.7 Control 4.1

Referring to Table 3, Example 1 (57.5%) using a strain derived from a traditional food had better collagenase inhibitory activity than the comparative example, and in particular, Comparative Examples 5 and 8 showing similar molecular weights of peptides (48.0 and 37.7, respectively. %) showed excellent collagenase inhibitory activity.

The above results suggest that by using strains derived from traditional foods instead of commercial enzymes as proteolytic enzymes of microalgae, it is possible to provide functional peptides having excellent whitening activity while ensuring safety and economy.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains will be able to understand that other specific forms can be easily modified without changing the technical spirit or essential features of the present invention will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and are not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be construed as being included in the scope of the present invention.

Claims (8)

Cosmetic composition comprising a peptide derived from microalgae. The method of claim 1,
The microalgae is a cosmetic composition of degreasing microalgae. The method of claim 1,
The size of the peptide is 1.4 kDa or less cosmetic composition. The method of claim 1,
A cosmetic composition in which the number of amino acids in the peptide is 13 or less. The method of claim 1,
The peptide is a cosmetic composition obtained by treating microalgae with enzymes. The method of claim 5,
The enzyme is a cosmetic composition obtained from a strain isolated from a fermented food. The method of claim 6,
The strain of Bacillus (Bacillus), Lactobacillus bacteria (Lactobacillus), Penny room Solarium (Penicillium), peusa Solarium (Fusarium), Pichia (Pichia), Candida (Candida), wrapped horizontally My process (Saccharomyces), Pseudomonas (Pseudomonas) , Citeromyces (Citeromyces), and aspergillus ( Aspergullus ) at least one cosmetic composition selected from the group consisting of. The method according to any one of claims 1 to 7,
The cosmetic composition is for skin whitening or skin regeneration.

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