KR20190059715A - A cosmetic composition comprising edible insect protein - Google Patents

Abstract

The present invention relates to a cosmetic composition including an edible insect protein as an active ingredient, for skin whitening or skin regeneration. The cosmetic composition of the present invention comprises peptide derived from larvae of a darkling beetle as an active ingredient, wherein the peptide has a size of 1.4 kDa or less.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition comprising an edible insect protein as an active ingredient,

The present invention relates to a cosmetic composition containing as an active ingredient a peptide derived from a larva of a brown duck.

Skin is the most basic defensive body that protects the body against external stimuli in contact with the external environment, and is an important institution expressing external beauty. Skin is not only a criterion of external beauty that symbolizes youth and health, but also a competitiveness that affects business ability by expressing self-confidence.

However, as the external activity of people increases, skin is exposed to sunlight for a long period of time and the skin is damaged by ultraviolet light. Prolonged exposure of the skin to ultraviolet light promotes skin aging and the skin becomes discolored due to the melanin pigment produced to protect the subcutaneous layer from ultraviolet light.

Therefore, consumption of functional cosmetics is increasing as a method of managing skin in a modern society, and whitening effect and prevention of aging are actively being developed in the cosmetics industry because white, clean and youthful elastic skin is preferred.

The whitening effect of cosmetics is to whiten the user's skin, and most whitening cosmetics function to inhibit the production of melanin. Melanin is produced by the melanocytes in the basal layer of the epidermis, and the activity and distribution of the melanocytes determine the skin color of a person.

Tyrosine, a type of amino acid, is converted to dihydroxy phenylalanine (DOPA) by an enzyme called tyrosinase contained in the melanocyte, and dopaquinone and dopachrome Through which a melanin, which is a polymer, is finally produced. Therefore, most whitening cosmetics inhibit the activity of tyrosinase, which is the main enzyme of melanin production, and thus, the whitening effect is exerted by the principle of inhibiting the production of melanin.

Anti-aging of cosmetics is to block or eliminate the causes that promote skin aging. The aging of the skin is photoaging due to sunlight, not a natural phenomenon as the age increases.

Photoaging is caused by excessive oxidation of antioxidants such as antioxidant enzymes in the body and vitamin E, C, glutathione and ubiquinol, resulting in the generation of excess reactive oxygen species by ultraviolet rays.

As the photoaging proceeds, not only the melanin production reaction is promoted but also damage of biological constituents by lipid peroxidation, protein oxidation, activation of proteolytic enzymes, chain cleavage and abnormal cross-linking of collagen and elastin, hyaluronic acid chain cleavage, And eventually skin aging such as skin elasticity reduction, wrinkles and spots, and the generation of freckles occur. Accordingly, the cosmetic composition having an antioxidative effect while inhibiting the activity of tyrosinase can effectively prevent whitening of the skin and prevent skin aging.

(Japanese Patent Laid-open No. 4-9320), hydroquinone (Japanese Patent Laid-open No. 6-192062), kojic acid (Japanese Patent Laid-open No. 56-7710), arbutin Rosinase inhibitory activity and was used as a whitening cosmetic. However, these ingredients are poor in stability among cosmetic formulations, may be decomposed to cause coloration or odor, and their safety or efficacy in vivo is unclear and their use is limited.

The cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

On the other hand, insects are known to be the most diverse species on the planet, and there are more than a million species currently identified. More than 1,900 insects are used for food, and these insects are called edible insects (edible insects).

Edible insects are composed of 50% protein and 30% fat, and 80% of the fat is unsaturated fatty acid. It is reported that edible insects, such as brown goats, which are easily available in Korea, have a high content of nutrients useful for skin such as minerals such as calcium and magnesium and unsaturated fatty acids.

However, edible insects are difficult to spread to food due to their resistance to food and the unpleasant odors of certain insects. Recently, a method of extracting useful nutrients contained in edible insects and converting them into high value-added functional materials has been studied.

Therefore, in view of the necessity of development of natural products derived from natural materials which are safe and functional in vivo, the present inventors extracted peptides from edible insects and confirmed their functionality and utilized them as natural functional cosmetic materials.

The object of the present invention is to provide a cosmetic composition containing peptides derived from larvae of edible insects and having excellent biostability as well as functionality.

According to one aspect of the present invention, there is provided a cosmetic composition comprising as an active ingredient a peptide derived from larva of a brown duck.

In one embodiment, the size of the peptide may be 1.4 kDa or less.

In one embodiment, the number of amino acids in the peptide may be 13 or less.

In one embodiment, the peptides can be obtained by treating the larvae of brown ducks with enzymes.

In one embodiment, the enzyme is selected from the group consisting of Alcalase, Protamex, Neutrase, Flavourzyme, Pepsin and Trypsin. It may be more than one kind.

In one embodiment, the enzyme may be obtained in a strain isolated from fermented food.

In one embodiment, the strain is Bacillus (Bacillus), Lactobacillus bacteria (Lactobacillus), Penny room Solarium (Penicillium), peusa Solarium (Fusarium), Pichia (Pichia), Candida (Candida), wrapped horizontally My process (Saccharomyces ), Pseudomonas (Pseudomonas), ssite may be at least one selected from the group consisting of Roman process (Citeromyces), and Aspergillus (Aspergullus).

In one embodiment, the cosmetic composition may be for skin whitening or skin regeneration.

Since the cosmetic composition according to one aspect of the present invention contains an active ingredient of a natural raw material, it is not only beneficial to skin health but also excellent in whitening and skin regeneration effect.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

FIGS. 1 to 3 are SDS-PAGE results of measuring the molecular weights of the peptides obtained according to one embodiment of the present invention.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a cosmetic composition comprising as an active ingredient a peptide derived from larva of a brown duck.

The brown goose ( Tenebrio molitor ) is an insect belonging to the goat family and is widely distributed throughout the world. It is known as a nocturnal insect inhabited mainly in grains. It is very high in protein content of 40-50%, and is popular for future food. Larva is called mealworm and used for food.

The brown larvae are dark brown in color and have a length of about 15 mm, and eggs laid by adults are hatched after 1-2 weeks. The hatching larvae become pupae after 9-20 hatchings and become adults. The brown goat larva is highly valued as a food material because of its high protein and fat content, and has been attracting attention as a future food resource. Furthermore, as a result of the stability test of the brown larvae, no significant toxicity was found when the mice were fed a dose of 3,00 mg / kg / day for one month in an animal test.

The above-mentioned " comprising as an active ingredient " is intended to mean an effective amount of an effective amount capable of exhibiting a skin improving effect, such as collagen synthesis associated with a wrinkle-reducing effect, elasticity improvement or skin whitening, skin irritation alleviation, It can mean to do.

The peptide is a compound formed by bonding two or more amino acids in a chain form or an annular form to the? -Carboxy group and the? -Amino group. The peptide is classified as a dipeptide, a tripeptide, a tetrapeptide or an oligopeptide or a polypeptide according to the number of amino acid bonds. The number of oligopeptides (2 to 10 amino acids bound) and polypeptides (10 to 50 amino acids) are not necessarily determined.

The size of the peptide may be 1.4 kDa or less, and the number of amino acids of the peptide may be 13 or less.

When the size of the peptide is 1.4 kDa or less, or the number of amino acids is 13 or less, the skin absorption rate can be increased because the size of the molecule is small, and the functionality can be improved when applied to a cosmetic product.

The peptides derived from the larva of the brown gill include a pretreatment step of grinding and freeze-drying the larva of the brown gill; A peptizing step of mixing a larva of a powdered brown duck with an enzyme solution containing a proteolytic enzyme; And a peptide separation step of filtering the mixture and obtaining a peptide by centrifugation.

In the pretreatment step of the above method, a protein high-concentration step may be further added in which the larva of the brown duck is pulverized and precipitated in a solvent (acid / base / organic solvent) to selectively extract the protein. Since the protein high concentration step can selectively degrade the protein only in the larva of the brown duck, the peptide production efficiency can be improved.

In the pre-treatment step of the above process, freeze-drying is carried out by sublimation of the ice directly into steam by lowering the partial pressure of the water wapor. If the residual moisture is kept below 2% w / w through the lyophilization, the pulverized material can be stored and transported stably without loss of useful material.

The proteolytic enzyme is an enzyme which hydrolyzes a protein in the peptidization step of the above production method, and is digested with the crushed brown larch larvae for a certain period of time to perform enzymatic degradation.

An enzyme such as Alcalase, Protamex, Neutrase, Flavourzyme, Pepsin and Trypsin, which are commercially available, can be used as the degrading enzyme . The pepsin (Pepsin) and trypsin (Trypsin) may be derived from animals.

Furthermore, the degrading enzyme is Bacillus (Bacillus), Lactobacillus bacteria (Lactobacillus), Penny room Solarium (Penicillium), peusa Solarium (Fusarium), Pichia (Pichia), Candida (Candida), wrapped horizontally My process (Saccharomyces), Pseudomonas Enzymes derived from microorganisms such as Pseudomonas , Citeromyces , and Aspergullus can be used. In addition, Fusarium < RTI ID = 0.0 > illudens , F. lateritium , F. solani , Paecilomyces sp ., Penicillium aurantiogriseum Dierckx, P. brevicompactum, P. citrinum, P. chrysogenum, P. corylophilum, P. crustosum, P. expansum, P. fellutanum, P. implicatum, P. roqueforti, P. solitum And P. Waksman .

The microorganism may be a food-derived food safety bacterium isolated from chungkukjang, miso, natto, soy sauce, kimchi and the like, and may be used as a commercially available fermentation broth and may be used for producing peptides.

In order to produce the proteolytic enzyme from the strain, a medium for the production of a liquid or solid phase enzyme is used. The culture medium for enzyme production is inoculated with the strain at 10% v / v, cultured at 25-30 ° C for 24 hours, centrifuged at a rate of 13,00xg for 20 minutes, and the supernatant is recovered and used as an enzyme solution .

In the peptizing step of the preparation method, the brown goat's larva larvae and the enzyme solution are mixed at a ratio of 1:30, the temperature condition is 20-60 ° C, and the reaction time is 2-24 hours. After the reaction, the enzyme was inactivated by heating in 95 ° C water for 10 minutes and centrifuged at 3,000xg for 20 minutes to obtain peptide supernatant Can be obtained.

The cosmetic composition may be used for skin whitening or skin regeneration.

The "whitening" suppresses the formation of various biomolecules such as melanin, redox hemoglobin, carotene and melanoid which affect skin color, thereby alleviating skin pigmentation phenomena such as dullness, freckles and stains, Thereby improving skin brightness and uniformity. In particular, the skin tone can be improved by inhibiting the activity of tyrosinase, which is a melanin pigment-forming enzyme.

The " skin regeneration " means prevention of skin aging, which means an effect such as prevention and improvement of skin wrinkles. Skin wrinkles are generated by collagen degradation, and collagenase, which is collagenase, is suppressed, thereby improving wrinkles of the skin.

The cosmetic composition may be at least one selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Can be formulated.

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; Liquid type, solid type or spray type, but the present invention is not limited thereto. In addition, the external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixtures may be used.

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example

The brown larvae used in the experiment were supplied from the insect farmers in Asan, Chungnam. The brown goat larvae were stored in a freezer at -50 ° C.

Brown larvae were crushed by a pulverizer, precipitated in distilled water at 30 ° C temperature to extract proteins, and lyophilized to obtain a water-soluble solid.

Example 1

Peptides were prepared by digesting the solids obtained in the Production Example.

1 g of the solid was mixed with 0.2 mol / l sodium phosphate buffer (SPB) at a ratio of 1:30 (w / v) and reacted for 8 hours at 200 rpm in a shaking incubator (SIB-05RH, Jeongbio, Korea).

After the reaction was completed, the enzyme was inactivated by heating at 100 ° C for 10 minutes, and then centrifuged at 3,000xg for 20 minutes to obtain a supernatant.

The resulting supernatant was filtered to remove impurities and lyophilized to obtain a solid sample.

Example 2

Example 2 was carried out in the same manner as in Example 1, except that Protamex was used as a digesting enzyme.

Example 3

Example 3 was carried out in the same manner as in Example 1, except that Neutrase was used as a degrading enzyme.

Example 4

Example 4 of the present invention was carried out in the same manner as in Example 1, except that flavorzyme was used as a degrading enzyme.

Example 5

Example 5 of the present invention was carried out in the same manner as in Example 1, except that Alcalase was used as a degrading enzyme.

Example 6

Example 6 of the present invention was carried out in the same manner as in Example 1, except that Chungkukjang strain enzyme solution was used as a degrading enzyme.

Table 1 below shows the results of comparing the proteolytic activity (protease activity) of the enzyme solution of chungkukjang strain used in Example 6 with alkaline.

division Protease activity (unit / ml) Alcazar 378.9 Chungkukjang strain enzyme solution 708.5

Comparative Example 1

Peptides were prepared by digesting the solids obtained in the Production Example.

1 g of solid and 1 mol of NaOH were mixed at a ratio of 1:30 (w / v), and the mixture was reacted for 8 hours at 50 ° C and 200 rpm in a shaking incubator (SIB-05RH, Jeongbio, Korea).

At the end of the reaction, the enzyme was inactivated by heating in 95 ° C water for 10 minutes and then centrifuged at 3,000xg for 20 minutes to obtain a supernatant.

The resulting supernatant was filtered to remove impurities and lyophilized to obtain a solid sample.

Comparative Example 2

Comparative Example 2 of the present invention was carried out in the same manner as in Comparative Example 1 except that the acid was decomposed with 1 mol H ₂ SO ₄ instead of the base decomposition.

Comparative Example 3

Comparative Example 3 of the present invention was carried out in the same manner as in Comparative Example 1, except that the acid was decomposed with 6 mol HCl at 110 deg. C for 24 hours instead of the base decomposition.

Experimental Example 1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the examples.

The samples of the examples were suspended in purified water at different concentrations (50 mg / L), and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added to each well, stirred for 10 minutes, and absorbance was measured at 540 nm.

Referring to Table 2, no cytotoxicity was observed at all concentrations of the samples of the respective Examples. The comparative sample was highly cytotoxic since it was obtained by base decomposition or acid decomposition.

The above results suggest that the sample of the embodiment is not only harmless to the human body but also has excellent human safety.

division Cell activity % ) Control group 98.3% Acid decomposition (HCl) 68.4% Base Decomposition (NaOH) 47.2% Enzyme decomposition (Alcalsase) 96.0% Enzyme derived from Chungkukjang 96.6%

Experimental Example 2: Measurement of Peptide Molecular Weight

SDS-PAGE was used to measure the molecular weight of the peptides contained in the samples of Examples and Comparative Examples.

SDS-PAGE gels were composed of 12-16.5% separating gel and 5% stacking gel.

Staining buffer was mixed with 0.1% Coomassie brilliant blue R-250, 45% methanol and 10% acetic acid, and de-staining buffer was mixed with 10% acetic acid and 45% methanol.

For the control, the brown goat larvae were suspended in 5 × SDS-sample buffer (0.25 mol Tris-HCl buffer, pH 6.8, containing 10% SDS, 4% 2-mercaptoethanol, 20% glycerol, 0.05% bromo-phenol blue and 10 mM EDTA ) And hot-water-extracted at 100 ° C for 5 minutes.

Standard molecular weight markers were used for SDS-PAGE molecular weight standards (Biorad, USA) and for polypeptide SDS-PAGE molecular weight standards.

After electrophoresis using the electrophoresis device, the gel was stained with staining buffer for more than 2 hours and decolorized with destaining buffer.

The results of peptide molecular weight measurement are shown in FIGS. 1, 2 and 3.

Referring to FIG. 1, bands were observed in the vicinity of 6.5 kDa in the control group. In Comparative Example 1, a draw phenomenon occurred due to base decomposition, and most of the peptides were present at less than 6.5 kDa.

In Comparative Examples 2 and 3, most of the peptides were not distributed between 6.5 and 200 kDa, and existed below 6.5 kDa. In particular, in Comparative Example 3, the protein was decomposed into a single amino acid by the process of decomposing into a single amino acid, and no band of 6.5 kDa or more appeared. Peptides of 6.5 kDa or less were also produced in Examples 1 to 5 using commercial enzymes, and no band was observed at 6.5 kDa or more.

Referring to FIG. 2, in Example 6, a band was observed in the vicinity of 25 kDa. As a result, it was confirmed that the casein included in the culture broth was compared with the culture broth of Chungkukjang strain and casein.

A band of about 6.5 kDa in the control was not present in Example 6, suggesting that the enzyme derived from the microorganism decomposes the peptides of the larvae of the larvae to 6.5 kDa or less.

Referring to FIG. 3, in Example 5 using the commercial degrading enzyme and Example 6 using the microbial degrading enzyme, no band appeared at over 3.5 kDa, and most of the protein was decomposed to below 1.4 kDa.

Experimental Example 3: Tyrosinase inhibitory activity test

The tyrosinase inhibition activity of the extracts of Examples and Comparative Examples was evaluated.

Tyrosinase is an enzyme that reacts with tyrosine to form melanin. When thyrosinase is inhibited, the production of melanin is inhibited and thus a whitening effect is exhibited.

In order to measure the inhibitory effect of tyrosinase of Examples and Comparative Examples, the DOPA Oxidase method was modified and used.

Buffer was prepared by adding 125 units / mL mushroom tyrosinase (Sigma-Aldrich Co., St. Louis, Mo.) to a mixture of 0.4 mL of 67 mmol sodium phosphate buffer (pH 6.8) and 0.2 mL of a 10 mM solution of 3,4-dihydroxy phenylanin , USA) and 0.2 mL of the sample.

The reaction was carried out at 37 ° C for 20 minutes, and the amount of DOPAchrome produced in the reaction solution was measured at a wavelength of 475 nm.

The sample was prepared by adding 0.2 mL of distilled water instead of the sample, reacting at 37 ° C for 20 minutes, and measuring at 475 nm using a spectrophotometer (UV-1650PC, Shimadzu, Kyoto, Japan).

The tyrosinase inhibitory activity was calculated by the ratio of the number of samples to the number of samples in accordance with the following formula (Table 3).

[Mathematical Expression]

(%) = 100 x (1 - (absorbance of each sample added group / absorbance of sample not containing group))

division Tairosineiz  Inhibitory activity % ) Example 1 23.4 Example 2 30.5 Example 3 25.2 Example 4 27.0 Example 5 40.3 Example 6 31.6 Comparative Example 1 16.4 Comparative Example 2 29.6 Comparative Example 3 33.8 Control group 0 Ascorbic acid 34.9

Referring to Table 3, Examples 1 to 5 using industrial degrading enzymes showed tyrosinase inhibitory activities similar to those of Comparative Examples 1 and 2. The higher the inhibitory activity of tyrosinase, the better the whitening effect.

In Comparative Example 3, which was single amino acid, the tyrosinase inhibitory activity was higher than that of Comparative Examples 1 and 2. The results suggest that the tyrosinase activity can be increased as the peptide is lowered in molecular weight.

The tyrosinase inhibitory activity of Example 5 using alkaline was used as the degrading enzyme, and the tyrosinase inhibitory activity of Example 6 using the enzyme solution of Chungkukjang was similar to that of Comparative Example 3.

These results suggest that the whitening effect of the peptides obtained with the commercial enzyme and the chrysanthemum strain enzyme solution in brown goat larvae is equivalent to that of the peptide obtained by base / acid decomposition.

Experimental Example 4: Collagenase inhibitory activity test

The collagenase inhibition activity of the extracts of Examples and Comparative Examples was evaluated.

Pro-Leu-Gly-Pro-D-Arg (Sigma, USA) dissolved in 0.3 mg / mL of 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Sigma, USA) was added to 4 mM CaCl ₂ in 0.1 M tris- μL was mixed with 50 μL of the sample.

To the mixed solution, 0.075 mL of 1 mg / mL collagenase from Clostridium histolyticum (Sigma-Aldrich Co, Louis, MO, USA) was added.

The sample was added with 50 μL of distilled water instead of the sample and allowed to stand at room temperature for 20 minutes. The reaction was stopped by adding 6% citric acid (0.5 mL), and 2 mL of ethyl acetate was added thereto. The resultant was analyzed with a spectrophotometer (UV-1650PC, Shimadzu, Kyoto , Japan). The absorbance at 320 nm was measured.

The collagenase inhibitory activity was calculated according to the following equation (see Table 4).

[Mathematical Expression]

Collagenase inhibitory activity (%) = 100 x (1 - (absorbance of each sample addition group / absorbance of sample not added group))

division Collagenase  Inhibitory activity % ) Example 1 16.3 Example 2 41.9 Example 3 31.8 Example 4 21.6 Example 5 66.7 Example 6 65.9 Comparative Example 1 16.5 Comparative Example 2 18.8 Comparative Example 3 34.0 Control group 11.2

Referring to Table 4, Examples 1 to 5 using industrial degrading enzymes showed collagenase inhibitory activity similar to Comparative Examples 1 and 2. The higher the collagenase inhibitory activity, the better the skin regeneration effect.

The single amino acid of Comparative Example 3 had higher collagenase inhibitory activity than that of Comparative Examples 1 and 2, suggesting that the collagenase activity can be increased as the peptide is reduced in molecular weight.

The collagenase inhibitory activity of Example 5 using alkaline was used as the degrading enzyme, and the collagenase inhibitory activity of Example 6 using the enzyme solution of Chungkukjang was similar to that of Comparative Example 3.

This result suggests that the skin regeneration effect of the peptides obtained using the commercial enzyme and the chrysanthemum strain enzyme solution in brown larvae is better than that of the peptide obtained by base / acid decomposition.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

A cosmetic composition comprising, as an active ingredient, a peptide derived from larva of a brown duck. The method according to claim 1,
Wherein the peptide has a size of 1.4 kDa or less. The method according to claim 1,
Wherein the peptide has 13 amino acids or less. The method according to claim 1,
Wherein the peptide is obtained by treating the larva of a brown duck with an enzyme. 5. The method of claim 4,
Wherein the enzyme is at least one selected from the group consisting of Alcalase, Protamex, Neutrase, Flavourzyme, Pepsin and Trypsin. 5. The method of claim 4,
Wherein the enzyme is obtained from a strain isolated from a fermented food. The method according to claim 6,
The strain of Bacillus (Bacillus), Lactobacillus bacteria (Lactobacillus), Penny room Solarium (Penicillium), peusa Solarium (Fusarium), Pichia (Pichia), Candida (Candida), wrapped horizontally My process (Saccharomyces), Pseudomonas (Pseudomonas) , Rome ssite process (Citeromyces), and Aspergillus cosmetic composition at least one member selected from the group consisting of Aspergillus switch (Aspergullus). 8. The method according to any one of claims 1 to 7,
Cosmetic composition for skin whitening or skin regeneration.

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