KR102486956B1 - A composition for skin whitening comprising enzymatic hydrolysates of protaetia brevitarsis larva - Google Patents

Abstract

The present invention relates to a composition for skin whitening containing an enzymatic hydrolyzate of the larvae of the white-spotted daisies. According to the present invention, the composition exhibited an excellent effect of inhibiting melanin production in melanoma cells, and thus can be used in external skin preparation compositions such as cosmetics for skin whitening purposes.

Description

A composition for skin whitening comprising enzymatic hydrolysates of protaetia brevitarsis larva

The present invention relates to a composition for skin whitening containing an enzymatic hydrolyzate of the larvae of the white-spotted rhododendron.

Human skin color is largely determined by the amount of melanin, hemoglobin, and carotene, among which melanin plays the most important role. Melanin not only determines human skin color but also plays a role in skin protection (ultraviolet ray absorption, free radical scavenger), but is excessively produced in the skin due to external environmental changes such as excessive exposure to ultraviolet rays, air pollution, and stress. When this happens, pigmentation occurs, which causes the skin color to become black or spots, freckles, etc. Among the external stimuli, UV rays are the largest melanin biosynthesis stimulants, and can affect various processes related to melanin production. That is, ultraviolet rays act as a major factor inducing excessive melanin production by promoting the activity of melanocytes, which are melanin-producing cells, promoting the secretion of melanin biosynthesis stimulating hormone, promoting melanin oxidation, or promoting tyrosinase activity.

The biggest feature of this melanin production mechanism is that only one enzyme called tyrosinase is involved, and when melanin production is prevented by inhibiting the tyrosinase activity, a whitening effect can be expected.

Most of the whitening agents developed so far reduce the amount of melanin by inhibiting the activity of tyrosinase, an enzyme that plays the most important role in melanin biosynthesis. Specifically, hydroquinone, hydroxyanisole, ascorbic acid derivatives, kojic acid, azelaic acid, corticosteroids known as tyrosinase inhibitors ( corticosteroids), retinoids, arbutin, etc., but it is difficult to use them due to problems such as safety and economic feasibility. In particular, hydroxyanisole and hydroquinone have strong melanogenesis inhibitory activity, but at the same time cause degeneration or death of pigment cells and exhibit side effects such as damaging the original function of cells. In addition, kojic acid has problems such as discoloration during use and instability of the material itself.

Recently, the Food and Agriculture Organization (FAO) has announced a plan to activate edible insects as a future food resource as a policy to solve the problem of food shortage due to disease or environmental pollution, and interest in this is increasing worldwide. In line with this, the Ministry of Food, Agriculture, Forestry and Fisheries announced the "Five-Year Comprehensive Plan for Insect Industry Promotion", and support for the insect industry has been provided accordingly.

Insects are the most diverse species on earth, with more than one million species estimated to remain unreported, even though more than one million species have been reported. Since ancient times, insects have been used for medicinal purposes, and among them, research results have been reported that silkworms, locusts, slugs, and centipedes are effective in treating chronic diseases such as diabetes, inflammatory diseases, liver diseases, and arteriosclerosis. In addition, research results confirming that insects can also be used as cosmetic compositions for whitening purposes have been reported continuously (Republic of Korea Patent No. 10-1429679).

Accordingly, while the present inventors were looking for a natural substance as a new material with excellent whitening effect without side effects in the body, they paid attention to insect extracts and experimented with their functions. As a result, in particular, insect hydrolase extracts are excellent in melanin production inhibitory effect. The present invention was completed by confirming the possibility of utilization as a natural material by discovering.

An object of the present invention is to provide a composition for skin whitening comprising an enzymatic hydrolyzate of insects as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for skin whitening comprising an enzymatic hydrolyzate of insects as an active ingredient.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

In order to achieve the above object of the present invention, the present invention provides a composition for skin whitening comprising an enzymatic hydrolyzate of insects as an active ingredient.

The enzymatic hydrolyzate of the insect may be one obtained from one or more insects selected from the group consisting of rhinoceros beetle, housefly, white-spotted radishes, brown mealworm, black soldier flies, silkworms, locusts, and crickets.

Insect enzymatic hydrolysates are Pepsin (PS), Trypsin (TS), Alcalase (AC), Neutrase (NT), Protease NP (PNP), Protamex (PMX), Pancreatin (P), Alphalase NP (ANP), Food Pro It may be produced by one or more proteolytic enzymes selected from the group consisting of Alkaline protease (AK) and Promod 278P (PM).

As used herein, "enzymatic hydrolysate" means a product after being hydrolyzed by one or more hydrolytic enzymes mentioned above. In a specific embodiment, the enzymatic hydrolyzate of the insect was hydrolyzed by treating the lyophilized insect pulverized material with the above-mentioned enzyme, and the resulting product was used in a skin whitening composition.

"Skin whitening" used in the present invention suppresses the production of various biomolecules that affect skin color, such as melanin, redox hemoglobin, carotene, and melanoids, thereby alleviating skin pigmentation such as blemishes, freckles, and melasma, It means the effect of improving skin brightness and uniformity by relieving yellowness and redness of the skin.

The insect enzyme hydrolyzate, which is an active ingredient of the skin whitening composition of the present invention, can be prepared through the following steps:

Preparing insect powder by lyophilizing and pulverizing insects;

Culturing after dissolving insect powder in distilled water;

Hydrolyzing the cultured insect powder by treating it with an enzyme; and

Provided is a method for preparing an enzymatic hydrolyzate of insects for skin whitening, comprising the steps of centrifuging the hydrolysis product, filtering the supernatant, and freeze-drying to prepare a final sample.

By inactivating the enzyme after the hydrolysis step, the enzyme may be inactivated so that the enzyme inherent in the final sample is no longer active when applied to the skin.

In addition, the present invention provides a cosmetic composition for whitening comprising an enzymatic hydrolyzate of insects as an active ingredient.

In the above composition, the content related to the enzymatic hydrolyzate of insects is the same as described above, so the description of the overlapping content will be omitted.

The cosmetic composition is at least one selected from the group consisting of softening lotion, nutrient lotion, moisture cream, nutrient cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder can be formulated.

In addition, the cosmetic composition may be formulated by conventional methods. In the formulation of external skin preparations such as cosmetics, reference may be made to the contents disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, and in the formulation of cosmetic compositions, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995) may be referred to.

Specifically, the cosmetic composition may be prepared in a general emulsified formulation and solubilized formulation. For example, cosmetic lotion, such as softening lotion or nourishing lotion; emulsions such as facial lotion and body lotion; creams such as nourishing creams, moisture creams, and eye creams; essence; cosmetic ointment; spray; gel; pack; sunscreen; makeup base; foundations such as liquid type, solid type or spray type; powder; makeup removers such as cleansing creams, cleansing lotions, and cleansing oils; Alternatively, it may be formulated as a cleanser such as a cleansing foam, soap, body wash, etc., but is not limited thereto. In addition, the cosmetic composition may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

In addition to the essential components in each formulation, the cosmetic composition may be appropriately mixed with other components within a range that does not impair the purpose of the present invention depending on the type of formulation or purpose of use.

The cosmetic composition may include a conventionally acceptable carrier, and for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, a preservative, a flavoring agent, and the like may be appropriately blended, but are not limited thereto. not.

The acceptable carrier may vary depending on the formulation. For example, animal oil, vegetable oil, wax, paraffin, starch, tracanthate, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or any of these as a carrier component when formulated into an ointment, paste, cream or gel mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component, and in the case of a spray, chlorofluorohydro It may further contain a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan may be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

When the cosmetic composition is formulated as a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. can be used

The cosmetic composition includes fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, and fragrances commonly used in the industry depending on the quality or function of the final product. , surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives commonly used in cosmetics It may additionally contain adjuvants commonly used in cosmetology or dermatology, such as any other ingredient.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect desirable properties of the cosmetic composition according to the present invention.

The present invention relates to a composition for skin whitening containing an enzymatic hydrolyzate of an insect. According to the present invention, since the composition exhibits a whitening effect by effectively inhibiting melanin production, it can be usefully used as a material for a cosmetic composition for skin whitening.

Figure 1 is a graph of the results of measuring the amount of melanin produced in melanoma cells according to the treatment of enzymatic hydrolysates of rhinoceros beetles, house flies, flower radishes, brown gourds, sandworms, silkworms, grasshoppers and crickets.
2 is a microscopic photograph of the amount of melanin produced in melanoma cells according to the treatment of enzymatic hydrolysates of rhinoceros beetles, house flies, flower radishes, brown gourds, rattlesnakes, silkworms, grasshoppers and crickets.
Figure 3 is a result showing the change in melanin production in melanoma cells (melanoma cells) according to the arbutin treatment by concentration.
4 is a melanoma cell by treating 5 types of insect enzyme hydrolysates of housefly (AC), flower nuisance (PNP), flower nuisance (Trypsin), brown gourd (Trypsin), and silkworm (Trypsin) by concentration. This is the result showing the change in melanin production.
5 is a result of confirming the change in the amount of melanin produced in melanoma cells by treating the hydrolyzate of PNP enzyme by molecular weight.
Figure 6 is a photograph of a microscopic observation of changes in melanin production in melanoma cells by treating PNP enzyme hydrolyzate by concentration and molecular weight.
7 is a result of confirming the cytotoxicity to melanoma cells by treating the hydrolyzate of PNP enzyme by molecular weight.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

[ Experimental example ]

Experimental example 1. Experimental Materials and Experimental Methods

1-1. sample preparation

Insects used in the test were a total of 8 types of insects, including 2 types of adults (grasshopper, cricket) and 6 types of larvae (brown mealworm, black soldier flies, white-spotted flower, rhinoceros beetle, housefly, silkworm), which are agricultural companies. It was purchased and used from Nabi Village, a corporation. Samples were purchased in a frozen state after washing, and were freeze-dried and used as test samples. After pulverization using a grinder, the powder was used for testing.

1-2. processing of proteolytic enzymes and hydrolyzate purchase

As for the proteolytic enzyme, Pepsin (PS) and trypsin (TS), which are single enzymes, were used by Sigma-Aldrich, and industrial enzymes Alcalase (AC), Neutrase (NT), Protease NP (PNP), Protamex (PMX), and Pancreatin (P), Alphalase NP (ANP), Food Pro Alkaline protease (AK), and Promod 278P (PM) were purchased and used.

After dissolving 10 mg of the insect meal prepared in Experimental Example 1-1 in 400 ml of distilled water, it was cultured for 6 hours by shaking culture. After centrifugation at 2000 rpm of the enzyme-treated solution that has undergone inactivation with a proteolytic enzyme under the treatment conditions in Table 1, the supernatant was filtered using a vacuum filter, and samples were prepared through freeze-drying. Specific information on each sample is shown in Table 2 below (PS: pepsin, TS: Trypsin, AC: Alcalase, P: Pancreatin, ANP: Alphalase NP, NT: Neutrase, PNP: Protease NP, PMX: Protamex, AK: Food Pro Alkaline protease, PM: Promod 278P)

Proteolytic enzyme treatment conditions division processing temperature
(℃) sample pH treatment concentration
(%) processing time
(h) Enzyme inactivation conditions untreated 50 7 - 6 80℃, 10min PM (Promod 278P) 50 7 One 6 80℃, 10min Alphalase NP (AP) 50 7 One 6 80℃, 10min Alcalase (AC) 50 7 One 6 80℃, 10min Neutrase (NT) 50 7 One 6 80℃, 10min pepsin (PS) 38 2 One 6 pH 7.5 Protamex (PMX) 50 7 One 6 80℃, 10min Pancreatin (P) 50 7 One 6 80℃, 10min Protease NPs (PNPs) 50 7 One 6 80℃, 10min trypsin (TS) 38 7 One 6 80℃, 10min

Enzymatically Treated Sample Label No. Name No. Name No. Name No. Name One Rhinoceros beetle (radish) 14 Flower Radish (Pepsin) 27 Same love (nothing) 40 Grasshopper (Pepsin) 2 Rhinoceros Beetle (AC) 15 flower plain (PMX) 28 Brotherhood (AC) 41 Grasshopper (PMX) 3 Beetle (Pepsin) 16 Flowers (AK) 29 Love, Love, and Love (Pepsin) 42 Grasshopper (AK) 4 Rhinoceros beetle (PMX) 17 Flower plain (PNP) 30 Same love (trypsin) 43 Grasshopper (PNP) 5 Rhinoceros beetle (AK) 18 Flower muji (trypsin) 31 silkworm (radish) 44 locust (trypsin) 6 Rhinoceros beetle (PNP) 19 Brown mealworm (radish) 32 Silkworm (AC) 45 Crickets (radish) 7 Rhinoceros beetle (trypsin) 20 Brown Goose (AC) 33 silkworm (pepsin) 46 Crickets (AC) 8 Housefly (No) 21 Brown mealworm (NT) 34 Silkworm (PMX) 47 Crickets (PMX) 9 Housefly (AC) 22 Brown mealworm (pepsin) 35 Silkworm (AK) 48 Crickets (AK) 10 Housefly (Pepsin) 23 brown mealworm PMX) 36 Silkworm (PNP) 49 Crickets (PNP) 11 Housefly (trypsin) 24 Brown mealworm (AK) 37 silkworm (trypsin) 50 Crickets (trypsin) 12 Flower plain (radish) 25 Brown mealworm (PNP) 38 Grasshopper (radish) 13 Flower Muji (AC) 26 Brown mealworm (trypsin) 39 Grasshopper (AC)

1-3. Purification of insect hydrolysates using ultrafiltration Method Insect protein hydrolysates were put into an ultrafiltration kit (manufactured by Amicon) equipped with a membrane filter (molecular weight cut-off: 100 kDa, 10 kDa, 5 kDa, 1 kDa), and 5 bar pressure was applied with nitrogen gas. It was added and fractionated into molecular weights of 100 kDa or more, 100 kDa to 10 k.Da, 10 kDa to 5 kDa, 5 kDa to 1 kDa, and 1 kDa or less, filtered, and the fractions were lyophilized.

1-4. B16F10 Confirmation of inhibition of melanin production by melanoma cells ( whitening activity evaluation)

(1) B16F10 melanoma culture

B16F10 melanoma (KCLB No. 80008) was purchased from Korea Cell Bank. Cells were cultured in DMEM medium supplemented with heat-inactivated 10% FBS and 100 unit/ml penicillin-streptomycin. The culture conditions were cultured in an atmosphere of 37°C and 5% CO 2 concentration, and the medium was replaced every 2 days.

(2) Analysis of melanin content in B16F10 melanoma

To measure the inhibitory activity of the samples on the melanin synthesis of B16F10 cells induced by α-MSH and IBMX, B16F10 melanoma was cultured in a 12-well cell culture plate.

Melanin accumulation in B16F10 melanoma was performed by adding 1 μM α-MSH, 200 μM IBMX, and samples for 72 hours. Medium was collected for extracellular melanin quantification and after washing the cells with PBS, the cells were incubated with 200 μL trypsin-EDTA. In order to measure the intracellular melanin content, the harvested cells were centrifuged at 27,237 g for 5 minutes. In addition, the cell pellet was dissolved in 200 μL of 1N NaOH and 10% DMSO at 100° C. for 10 minutes. The absorbance of intra- and extracellular melanin content was measured at 405 nm with a micro plate reader. Melanin content was calculated as a percentage of untreated cells.

1-5. insect enzymes hydrolyzate B16F10 Evaluation of cytotoxicity against melanoma cells

To measure the cell viability of the sample, MTT assay was performed on B16F10 melanoma. After sufficient cell division, the cells were treated with samples by concentration (10, 50, 100, 200 μg/mL, etc.) for 24 hours, then the medium was removed, and 0.2mg/ml MTT-medium (10% FBS DMEM with 100 unit/mL penicllin-streptomycin and 1 mL of MTT reagent (pH 7.4) dissolved in PBS were added per well, and the cells were incubated for 30 minutes.The reaction medium was removed, and insoluble formazan inside the cells was dissolved in 300 μL DMSO microroplate reader The absorbance of each cell was measured at 570 nm, and cell viability was calculated as a percentage of untreated cells.

[ Example ]

Example 1. 50 samples whitening activity (Melanin production inhibition ability) confirmed

For 50 samples prepared by enzymatic hydrolysis of 8 types of insects, B16F10 Melanoma cells were treated at a concentration of 50 μg/ml to confirm the ability to inhibit melanin production. Among the 50 samples, when house flies were hydrolyzed with AC, -22.2% compared to the control, when white-spotted daisies were hydrolyzed with PNP, -3.3%, when hydrolyzed with trypsin, 0.4%, and brown mealworms were hydrolyzed with trypsin. 3.2% when silkworms were hydrolyzed with trypsin, 1.1% of melanin pigment was produced, confirming strong whitening efficacy (Table 3, Figures 1 and 2).

insect enzymatic hydrolyzate whitening activity ( B16F10 Melanoma in cell amount of melanin produced) Melanin pigment content (% of control) beetle housefly flowers brown mealworm in camaraderie silkworm Grasshopper cricket untreated 29.5 34.3 17.6 17.4 32.9 35.5 24.3 15.3 AC 29.5 -22.2 40.8 57.3 31.7 34.7 33.0 25.6 pepsin 26.9 52.8 8.0 43.6 33.6 34.7 34.4 - PMX 12.2 - 23.3 68.0 - 28.2 29.9 31.8 AK 43.1 - 9.8 53.5 - 27.3 30.5 35.2 PNP 35.1 - -3.3 51.4 - 10.0 28.7 30.3 trypsin 14.2 10.2 0.4 3.2 29.3 1.1 18.5 29.3 NT - - - 96.7 - - - -

Example 2. whitening activity of 5 excellent species and arbutin whitening activity compare

In Example 1, 5 samples (Housefly-AC hydrolysate, white-spotted flower-PNP hydrolysate, white-spotted flower-trypsin hydrolyzate, brown gourd-trypsin hydrolyzate, silkworm- Trypsin hydrolyzate) was treated with B16F10 Melanoma cells at concentrations of 10, 25, and 50 μg/mL to test the inhibition of melanin production, and the inhibition rate of melanin production treated with arbutin, a representative whitening material, at each concentration was analyzed and compared.

Compared to arbutin, which showed a melanin production rate of 73.5% at a concentration of 13.6 μg/mL and 71.1% at a concentration of 27.2 μg/mL, arbutin (Table 4 and FIG. 3), PNP of white-spotted flower 27.9 at a concentration of 10 μg/mL %, 25 μg/mL concentration showed 0.6% melanin production rate, and housefly AC showed 35.4% at 10 μg/mL concentration. The melanin production rate was -23.0% at 25 μg/mL concentration, and 53.2% at 10 μg/mL, 13% at 25 μg/mL concentration, and 56.2% at 10 μg/mL, 25 μg silkworm trypsin at 10 μg/mL concentration. /mL concentration, 7.2%, brown gourd trypsin showed a melanin production rate of 87.1% at 10 μg / mL concentration and 20.2% at 25 μg / mL concentration (Table 5 and Figure 4), compared to arbutin used as a control It was found that the whitening effect (melanin production inhibition rate) was more excellent.

Production of melanin pigment by concentration of arbutin inhibition (( B16F10 Melanoma in cell amount of melanin produced) Melanin content (% of control) Arbutin concentration
(μg/ml) 0.3 1.4 2.7 6.8 13.6 20.4 27.2 68.0 136 272 Amount of melanin production ( % of control) 73.5 76.0 71.1 36.3 19.9 13.4

Insect Enzyme Hydrolysate 5 kinds of melanin pigment production by concentration inhibition ( B16F10 Amount of melanin produced in melanoma cells) Melanin content (% of control) 10 (μg/mL) 25 (μg/mL) 50 (μg/mL) Housefly (AC) 35.4 -23.0 -30.7 White-spotted flower plain (PNP) 27.9 0.6 -12.8 White-spotted flower bush (Trypsin) 53.2 13.0 -3.6 Mealworm (Trypsin) 87.1 20.2 2.6 Silkworm (Trypsin) 56.2 7.2 1.6

Example 3. white-spotted flower - PNP enzymatic hydrolyzate by molecular weight fractional whitening activity compare

In the sample hydrolyzed with the enzyme PNP, the amount of melanin produced in melanoma cells at a sample concentration of 50 μg/mL was 79.7% at less than 1 KDa, 66.6% at 1 to 5 KDa, 69.6% at 5 to 10 KDa, and 10 As 46.4% at ~100 KDa and 68.8% at 100 KDa or more, the whitening activity of inhibiting melanin production was the best at 10-100 KDa (Table 6, FIGS. 5 and 6).

white-spotted flower - PNP enzymatic hydrolyzate by molecular weight whitening activity ( B16F10 Amount of melanin produced in melanoma cells) Melanin contents (% of control) 10 (μg/mL) 50 (μg/mL) ~ 1 kDa 79.1 ± 0.83 79.7 ± 0.62 1~5 KDa 105.2±0.46 66.6 ± 0.95 5~10 KDa 76.3 ± 0.61 69.6 ± 0.82 10 to 100 KDa 79.2 ± 0.44 46.4 ± 0.09 100 KDa ~ 75.7 ± 0.19 68.8 ± 0.54

Example 4. white-spotted flower - PNP enzymatic hydrolyzate whitening cells toxicity test

The enzymatic hydrolyzate of white-spotted flower radish PNP was separated into 5 stages: 1 KDa or less, 1 to 5 KDa, 5 to 10 KDa, 10 to 100 KDa, and 100 KDa or more through ultrafiltration to obtain 10, 50, 100, 200 μg/ As a result of the cytotoxicity test at the mL concentration, it was found that the cell viability was at least 83.2% and there was no cytotoxicity (Table 7 and FIG. 7).

white-spotted flower - PNP enzymatic hydrolyzate Results of cytotoxicity test by molecular weight ( B16F10 Cytotoxicity test for melanoma cells) Fractions by molecular weight Cell viability (% of control) (μg/mL) 10 (μg/mL) 50 (μg/mL) 100 (μg/mL) 200 (μg/mL) ~ 1 kDa 88.9 ± 1.21 83.2 ± 4.25 86.6 ± 7.47 86.0 ± 10.09 1~5 KDa 93.1 ± 5.41 97.8 ± 6.71 94.5 ± 2.34 109.1 ± 8.49 5~10 KDa 102.4 ± 6.04 101.1 ± 9.21 95.1 ± 8.14 90.4 ± 5.26 10~100 KDa 92.8 ± 3.76 87.8 ± 5.22 89.1 ± 9.10 87.5 ± 5.65 100 KDa ~ 91.3 ± 6.34 90.3 ± 4.32 93.0 ± 9.76 102.7 ± 7.74

Summarizing the above experimental results, the enzymatic hydrolyzate according to the insects of the present invention could effectively inhibit melanin production in melanoma cells, respectively, and thus could be used for skin whitening purposes.

Claims (4)

Skin containing an enzyme hydrolyzate of white-spotted flower larvae as an active ingredient, wherein the enzyme is at least one selected from the group consisting of Pepsin (PS), trypsin (TS), Protease NP (PNP) and protease (AK) A composition for whitening. According to claim 1,
The composition is to inhibit the production of melanin pigment in melanoma cells, skin whitening composition. A cosmetic composition for skin whitening comprising the composition of claim 1 or 2. Freezing and pulverizing white-spotted flower larvae to prepare a powder;
Culturing after dissolving the powder in distilled water;
hydrolyzing the cultured powder by treating it with at least one enzyme selected from the group consisting of Pepsin (PS), trypsin (TS), Protease NP (PNP) and protease (AK); and
A method for preparing a composition for skin whitening comprising the steps of centrifuging the enzyme hydrolyzate, filtering the supernatant, and freeze-drying to prepare a final sample.

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