KR100524184B1 - Cosmetic Composition for Skin Whitening Comprising Myrothecium verrucaria-Cultured Medium as Active Ingredient - Google Patents

Abstract

The present invention provides a composition comprising a culture of a maze potassium chopping Luca Liao (Myrothecium verrucaria). The culture solution of myrcium berucaria of the present invention has an improved inhibitory effect on tyrosinase activity and an excellent effect of inhibiting melanin production of melanocytes compared to the conventional whitening ingredients, and a cosmetic composition comprising the culture solution of myrosium berucaria of the present invention. The composition exhibits an excellent skin lightening effect. In addition, the culture solution of myrcium berucaria of the present invention hardly irritates the skin.

Description

Cosmetic composition for skin whitening comprising a culture solution of myrcium berucaria {Cosmetic Composition for Skin Whitening Comprising Myrothecium verrucaria-Cultured Medium as Active Ingredient}

The present invention relates to a composition comprising a culture solution of myrothecium verrucaria , and more particularly, to a cosmetic composition for skin whitening comprising a culture solution of myrcium berucaria as a whitening component.

The color of human skin, hair and eyes is determined by melanin, carotene and hemoglobin, and the like. In particular, melanin is the most important factor determining skin color, and skin color is determined according to the amount, nature and distribution of melanin.

Human skin and hair pigments protect skin and hair from the harmful effects of sunlight, especially ultraviolet light. For example, people who lack these pigments are very sensitive to sunlight and are prone to burns and have a high probability of developing skin cancer even at a young age. Short wavelength ultraviolet (290-320 nm) and carcinogens form harmful radicals in the skin, such as oxygen radicals, which are known to attack skin cells and age the skin.

The main function of melanin is to remove these harmful radicals and protect the skin from the damage thereby. Thus, high amounts of melanin means that it has an effective response system that can protect the skin from physical or chemical toxic substances. Factors that promote the production of such melanin include sunlight (ultraviolet rays) as well as estrogen or prostaglandin.

Melanin is produced through the complex oxidation and condensation reaction of melanocytes in the melanocytes by tyrosine is converted to dopa, dopachrome by an enzyme called tyrosinase. The resulting melanin is delivered to the skin cells and exhibits a circulating action in which the melanin is lost and disappeared along with epidermal detachment.

This melanin production process is a naturally occurring phenomenon, and overproduction of melanin does not occur in normal human skin. However, when the skin responds to external stimuli such as ultraviolet rays, environmental pollution or stress, excessive production of melanin. If excess melanin remains in the skin rather than drains out of the skin, pigmentation occurs.

Among the external stimulating factors, UV is the largest melanin biosynthetic stimulus, and may affect various processes related to melanin production. That is, ultraviolet rays act as an important factor inducing melanin overproduction by promoting the activity of melanocytes, melanocytes, melanin biosynthesis stimulating hormone secretion, melanin oxidation, or tyrosinase activity.

The most distinctive feature of the melanin production mechanism is that only one enzyme called tyrosinase is involved. When the tyrosinase activity is inhibited to prevent melanin production, a whitening effect can be expected.

On the other hand, the method of suppressing melanin production in the whitening cosmetics can be largely divided as follows.

First, by inhibiting the melanin production by blocking the ultraviolet rays which is the main cause of melanin production, this method can be expected to contain a light scattering agent or a light blocker in the cosmetic composition, a good result can be expected. Second, a substance that inhibits the synthesis of core carbohydrates required for the activity of tyrosinase, such as glucosamine, is used to inhibit melanin production. Third, a method of inhibiting the activity of tyrosinase involved in melanogenesis using a substance such as enzyme acid (kojic acid) or arbutin. Fourth, a method of preventing cell division of melanocytes by using a substance having specific toxicity to melanocytes, which are cells producing melanin, such as hydroquinone. Fifth, it is a method of inhibiting melanin production using a substance exhibiting a strong antioxidant effect, such as vitamin C, during tyrosine undergoing complex oxidation and condensation reaction through dopa and dopachrome.

In the prior art, it is known to use ascorbic acid as a whitening substance (Japanese Patent Laid-Open No. 4-9320). However, ascorbic acid is poor in phase stability in the formulation, there is a problem that the activity of the active ingredient is lowered over time. Therefore, in recent years, various methods such as stabilizing ascorbic acid with capsules or liposomes have been proposed, but have not yet provided a clear stabilization method.

It is known to use hydroquinone as another whitening substance (Japanese Patent Laid-Open No. 6-192062). Hydroquinone has an excellent effect but is limited to its use as a cosmetic since it is a carcinogenic substance.

It is known to use kojic acid as another whitening substance (Japanese Patent Laid-Open No. 56-7710). Kojic acid has an excellent inhibitory ability of tyrosinase and thus shows an excellent whitening effect, but also has a problem in terms of safety in the formulation and has recently been announced as a carcinogen.

It is known to use arbutin as another whitening substance (Japanese Patent Laid-Open No. 4-9315). Arbutin can be extracted or synthesized from alpine bilberry and its ability to inhibit tyrosinase, like kojic acid, has been demonstrated. However, arbutin itself has a problem in that the sugar is attached to the hydroquinone, and when applied to the cosmetic, the sugar is separated by the skin enzyme to cause skin irritation by the hydroquinone.

Throughout this specification, numerous patent documents and articles are referenced and their citations are indicated. The disclosures of cited patent documents and articles are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors produce a material having a whitening effect on the microorganisms parasitic in marine algae in order to overcome the problems of conventional cosmetic composition for skin whitening, that is, lowering stability, side effects and weakening of the whitening effect, etc. As a result of screening the microorganisms to be screened, it is possible to screen out the myrcium berucaria , and in particular, the culture solution of the myrothesis berucaria shows an excellent inhibitory effect on tyrosinase activity and melanin production of melanocytes. It was confirmed that the present invention was completed.

Accordingly, it is an object of the present invention to provide a composition for inhibiting tyrosinase activity comprising a culture solution of myrcium berucaria as an active ingredient.

Another object of the present invention is to provide a composition for inhibiting melanin production of melanocytes comprising a culture solution of myrcium berucaria as an active ingredient.

Still another object of the present invention is to provide a cosmetic composition for skin whitening comprising a culture solution of myrcium berucaria as an active ingredient.

Other objects and advantages of the invention will be apparent from the following examples and claims.

According to one aspect of the present invention, the present invention provides a composition for inhibiting tyrosinase activity, comprising a culture solution of myrcium berucaria as an active ingredient.

According to another aspect of the present invention, the present invention provides a composition for inhibiting melanin production of melanocytes comprising a culture solution of myrcium berucaria as an active ingredient.

The present invention is characterized in that it comprises a culture solution of myrcium berucaria as a whitening component.

Mirosium berucaria used in the present invention is a fungus registered in KCCM 32013 and ATCC 9095 and is mainly present in soil. The fungus is formed of cellulase (Formation of cellulase and degradation of cellulose by several fungi. J. Ferment. Technol . 56: 273-278 (1978)) and endo-1,4-beta-cyanase (Physicochemical and catalytiic properties of a Low-molecular-weight endo-1,4-D-xylanase from Myrothecium verrucaria.Enzyme Microbs.Fed.Test Method Std. 191A).

Mirosium berucaria used in the present invention can be used separately from soil or algae, and can also be obtained from KCCM (32013) and ATCC (9095).

The culture medium of the myrcium berucaria used in the composition of the present invention is a culture solution after removing the fungus after culturing the myrothesis berucaria .

According to a preferred embodiment of the present invention, the medium used for culturing myrcium berucaria in the present invention may be a conventional medium used for culturing fungi, preferably YPM medium (yeast extract 0.2%, Peptone 0.2%, mannitol 0.4% and seawater 99.2%) or SWS medium (1.0% soluble starch, 0.1% soyton and 98.9% seawater). A more preferred medium used to prepare a culture solution of myrcium berucaria contained in the composition of the present invention is SWS medium.

According to a preferred embodiment of the present invention, the conditions for culturing myrcium berucaria in the present invention can use the conventional culture conditions used to culture the fungus, preferably 24-36 ℃ and humidity 30-80% More preferably 28-29 ° C. and humidity 50-60%.

According to a preferred embodiment of the present invention, the method for separating fungus from the culture medium in the present invention may be used a conventional separation method, preferably by filtration or centrifugation, etc., more preferably by filtration. Conventional filtration filters may be used for the filtration.

When referring to the following Examples of the present invention a manufacturing method of the maze potassium chopping Luca Liao culture medium of the following: algae, preferably a flat laver (Enteromorpha compressa) a sterile fungus maze by subculturing on solid media potassium Vespa Separate Lucaria . The isolated myrcium berucaria is incubated in YPM medium for several days at an appropriate temperature and humidity and then incubated for several weeks under the same conditions in SWS medium. The culture is filtered with a filter to separate the mycelium.

The present invention is characterized in that the culture medium of the myrcium berucaria is used as a skin whitening component, and the culture solution of the myrosium berucaria of the present invention inhibits the activity of tyrosinase, thereby producing melanin from tyrosine. It inhibits the production of dopaquinone from L-dopa, which is an intermediate step of the melanin production process, and also inhibits melanin production of melanocytes.

The composition of the present invention comprises a culture of an effective amount of myrcium berucara that can inhibit tyrosinase activity or inhibit melanin production of melanocytes. The content of the culture medium of the maze potassium chopping Luca Ria in a composition of the invention is 0.001-15.0% by weight based on the total weight of the composition, preferably 1.0-15.0% by weight, more preferably 5.0-10.0 wt%, most preferably 7 wt. %to be. If the content of the culture medium of myrcium berucaria is less than 0.001% by weight, there is a problem that the improvement of the whitening effect through inhibition of tyrosinase activity and inhibition of melanin production of melanocytes is slightly insufficient. There is a problem that the apparent increase in whitening effect is not seen as the content is increased.

According to another aspect of the present invention, the present invention provides a cosmetic composition for skin whitening comprising a culture solution of myrcium berucaria as an active ingredient.

Since the cosmetic composition of the present invention includes the above-mentioned culture medium of the myrcium berucaria as an active ingredient, in order to avoid excessive complexity of the present specification, duplicated descriptions are omitted.

The components included in the cosmetic composition of the present invention are components commonly used in cosmetic compositions in addition to the culture solution of myrcium berucaria as the active ingredient, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. Conventional adjuvants, and carriers and the like.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsion may be used as the carrier component. For example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan and the like can be used.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition comprising the culture solution of myrosium berucaria of the present invention exhibits excellent skin whitening effect through the inhibition of tyrosinase activity and the inhibition of melanin production of melanocytes, and has excellent skin safety because of low skin irritation. .

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Example

material

Mirothesis berucaria used in the following example was selected by the following method.

YPG + Agar solid medium (Yeast extract 0.5%, peptone 0.5%, glucose 1.0%, agar 1.6%, penicillium 0.1% and seawater) sterilized with Enteromorpha compressa collected from Busan Baekpo for 15 minutes at 120 ℃ 96.3%) was passaged three times to isolate the fungus myrcium berucaria .

The isolated myrcium berucaria was incubated in 10 ml of YPM medium (0.2% yeast extract, 0.2% peptone, 0.4% mannitol, 99.2% seawater) for 5 days at a temperature of 28-29 ° C. and a humidity of 50-60%. It was inoculated in 250 ml SWS medium (1.0% soluble starch, 0.1% soyton and 98.9% seawater) and incubated for 4 weeks under the same conditions as above.

The culture solution was filtered with a gauze and filtered again with a 0.45 μm filter to separate the mycelium, and then the filtrate, that is , a culture solution of myrcium berucaria, was used in the cosmetic composition.

Experimental Example 1: Mirotesium Berukaria Inhibition of Tyrosinase Activity in Culture Medium

40 μl of 1.5 mmol / L tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used as the substrate. A culture solution of myrcium berucaria was diluted with 0.05 M sodium phosphate buffer (pH 6.8) to prepare a sample solution for each concentration. 240 μl of the sample solution for each concentration was added to 40 μl of tyrosine, and 20 μl of tyrosinase (Sigma, 1500 U / ml) was added to the solution, followed by reaction at 37 ° C. for 15 minutes, and then at 490 nm. Absorbance was measured. As a control, one without addition of a culture solution of myrcium berucaria was used. In order to confirm the influence of the medium used for the cultivation of myrcium berucaria, the same experiment was conducted using the medium for the culture of myrcium berucaria . Inhibitory activity of tyrosinah was calculated by Equation 1. The results are shown in Table 1.

% Inhibition = [1-absorbance of comparative group (O.D.490) / control absorbance (O.D.490)] × 100

Concentration (%) of Mirothesis Berukaria % Inhibition Concentration (%) of cultivation medium of Mirotesium berucaria % Inhibition 0.0 0 0.0 0 0.1 19.8 0.1 0 0.2 40.1 0.2 0 0.8 76.7 0.8 0 2.0 88.8 2.0 0 5.0 92.8 5.0 0

As can be seen in Table 1 , the culture medium of myrcium berucaria showed an excellent inhibitory effect on tyrosinase activity, and the inhibition was increased depending on the culture solution concentration of myrothesis berucaria . Therefore, it can be seen that the composition containing the culture solution of myrcium berucaria of the present invention exhibits an excellent inhibitory effect on tyrosinase activity, and the effect of the culture solution of myrosium berucaria is higher than that of the conventional whitening agent, the upper limb extract. It was excellent.

In addition, since the medium used for culturing myrcium berucaria did not show tyrosinase inhibitory activity, it was found that the effect was not due to the components of the culture medium.

Experimental Example 2: Mirotesium Berukaria Melanin production inhibitory experiments in culture medium (Melanin assay)

B-16 cells (rat melanoma cells, Korea Cell Line Bank) were seeded in 12-well plates to 10 ⁴ cells / well and cultured for one day. Each well was treated with a culture solution of myrcium berucaria by concentration and incubated for 3-4 days, and the culture solution of each well was centrifuged. The isolated cells were lysed with 500 μl of 1N NaOH solution dissolved in dimethyl sulfoxide (DMSO) and heated in a 90 ° C. water bath for 10 minutes. It was centrifuged again and the absorbance of the supernatant was measured at 570 nm. As a control, one that was not treated with a culture solution of myrcium berucaria was used. In order to confirm the influence of the medium used for the cultivation of myrcium berucaria, the same experiment was conducted using the medium for the culture of myrcium berucaria .

Melanin production inhibition was calculated according to the following equation (2), the results are shown in Table 2.

% Inhibition = [1-absorbance of comparative group (O.D.490) / control absorbance (O.D.490)] × 100

Concentration (%) of Mirothesis Berukaria % Inhibition Concentration (%) of cultivation medium of Mirotesium berucaria   % Inhibition 0 0.0 0.0 0.0 0.1 19.8 0.1 0.0 0.2 40.1 0.2 0.0 0.4 60.1 0.4 0.0 0.8 76.7 0.8 0.0 2.0 88.8 2.0 0.0 5.0 92.8 5.0 0.0

As shown in Table 2, the culture medium of the maze potassium chopping Luca Ria has had an excellent effect on melanogenesis inhibition of melanoma cells, and the melanin synthesis inhibition is increased dependent on the concentration of the culture medium maze potassium chopping Luca Liao. Therefore, it can be seen that the composition comprising the culture solution of the myrcium berucaria of the present invention has an excellent effect on inhibiting melanogenesis.

In addition, since the medium used for culturing myrcium berucaria did not show melanin production inhibitory activity, it was found that the effect was not due to the components of the culture medium.

Experimental Example 3: Mirotesium Berukaria Inhibition of L-dopa Oxidation in Culture Media

850 μl of 50 mM Tris-HCl buffer, pH 7.5, previously maintained at 37 ° C. for 10 minutes, was placed in a 1 ml EP tube, and the mushroom tyrosinase (Sigma, United States, 18 units) and the test solution, myrosium beruka. to match the culture medium of the RIA it was added to a concentration 50 ㎕. This solution was left at 37 ° C. for 2 minutes and prepared immediately before using 15 mM L-dopa (Sigma, USA) and 50 μl was added. Immediately after addition, the absorbance change was measured for 1 minute at 475 nm using an ELISA reader. This measures the conversion of L-dopa to dopachrome, a brown product, by tyrosinase. As a control, one without addition of a culture solution of myrcium berucaria was used. In order to confirm the influence of the medium used for the cultivation of myrcium berucaria, the same experiment was conducted using the medium for the culture of myrcium berucaria . Inhibition was calculated according to the following equation (3), the results are shown in Table 3.

% Inhibition = [1-control absorbance (O.D.475) / control absorbance (O.D.475)] × 100

% Concentration of myrcium berukaria broth % Inhibition Concentration (%) of cultivation medium of Mirotesium berucaria % Inhibition 0 0 0.0 0.0 One 49.8 1.0 0.0 2.5 56.8 2.5 0.0 5 68.8 5.0 0.0 7.5 69.8 7.5 0.0 10 74.5 10.0 0.0

As can be seen in Table 3 , the culture medium of myrcium berucaria showed an excellent L-dopa oxidation inhibitory effect and the inhibition was increased depending on the concentration of the culture medium of myrcium berucaria . Therefore, it was found that the composition containing the culture solution of myrcium berucaria of the present invention showed an excellent effect in inhibiting L-dopa from being converted into dopachrome by tyrosinase.

In addition, since the medium used for culturing myrcium berucaria did not show L-dopa oxidation inhibitory effect, it was found that the effect was not due to the components of the culture medium.

Experimental Example 4: Mirotesium Berukaria Cytotoxicity Tests of Cultures (MTT Assay)

Methyltriazolyltetrazolium (MTT) analysis confirmed the toxicity of the culture medium of myrcium berucaria .

Fibroblasts (Korea Cell Line Bank) were seeded in 96-well plates at 8000 cells / well and incubated in 200 μl DMEM (Dulbecco Minimum Essential Medium) for 24 hours. The wells of myrcium berucaria were added to final concentrations of 0, 0.2, 0.8, 1.5, 3.0 and 5.0 μg / ml, respectively, and left for 20 hours. The mixed solution of the wells was left in a medium containing 0.5 mg / ml MTT solution for 4 hours, and then the medium was removed and 200 μl of DMSO solution was added to the wells for reaction. The reaction solution of the wells was measured for absorbance at 570 nm using an ELISA reader, and the cell proliferation rate was calculated according to the following Equation 4 with a control group (untreated group of myrcium berucaria ) at 100%. The results are shown in Table 3.

Cell proliferation rate = (absorbance of experimental group / absorbance of control group) × 100 (%)

Concentration (%) of Mirothesis Berukaria Cell growth rate (%) 0.0 100.0 1.5 104.2 3.0 106.5 8.0 106.8 15.0 107.1 20.0 105.6

As shown in Table 4 , the culture medium of myrcium berucaria did not show cytotoxicity against fibroblasts even at relatively high concentrations, and thus, the culture solution of myrotes berucaria was not cytotoxic.

Formulation Example 1 Flexible Lotion

To the flexible cosmetic lotion of Example 1 and Comparative Example 1 was prepared in the composition of Table 5.

ingredient Content (% by weight) Example 1 Comparative Example 1 Culture medium of myrcium berukaria acetic acid tocopherol oleyl alcohol ethanol benzophenone-9 carboxyvinyl polymer cellulose gum preservative 7.05.21.53.22.01.03.5 Minor balance -5.21.53.22.01.03.5 system 100 100

Formulation Example 2: Milk Lotion

To the milk lotion of Example 2 and Comparative Example 2 was prepared in the composition of Table 6.

ingredient Content (% by weight) Example 2 Comparative Example 2 Labyrinth potassium chopping Luca Ria the culture medium of glycerin, propylene glycol Tocopheryl Acetate Liquid paraffin Squalane triethanolamine macadamia nut oil polysorbate 60 sorbitan process kwirol rate propylparaben carboxylic polymer attitude subtitle purified water 7.05.14.23.04.61.03.12.51.61.60.61.5 Microbalance -5.14.23.04.61.03.12.51.61.60.61.5 Microbalance system 100 100

Formulation Example 3: Nutrition Cream

To the nutrition cream of Example 3 and Comparative Example 3 was prepared in the composition of Table 7.

ingredient Content (% by weight) Example 3 Comparative Example 3 Labyrinth potassium chopping culture glycerol vaseline Lucca Ria triethanolamine liquid paraffin, squalane wax tocopheryl acetate, polysorbate 60 carboxylic polymer sorbitan process kwiol rate attitude subtitle purified water 7.04.03.52.15.33.02.65.43.21.03.1 Microbalance -4.03.52.15.33.02.65.43.21.03.1 Microbalance system 100 100

Experimental Example 5: Whitening effect experiment of the cosmetic composition

A whitening effect test of the cosmetic composition of the present invention was carried out on 30 Korean women aged 19-40 years, especially those with dark skin. Minolta CR 300 was used as a measuring instrument and measured for 8 weeks at two-week intervals. The measurement site was marked with 1 cm by 9 cm on the arm and steadily applied different prescriptions (Examples 2, 3 and Comparative Examples 2, 3) to 8 places and one place was used as a control value for comparison.

The results of this experiment are shown in Table 8 below.

division Example 2 Example 3 Comparative Example 2 Comparative Example 3 Control △ L value 2.72 2.50 0.50 1.00 0.00

As shown in Table 8, when the ΔL value after 8 weeks, Example 2 and Example 3 was significantly higher than Comparative Examples 2, 3 and the control group. Therefore, it can be seen that the cosmetic composition of the present invention has a very good whitening effect.

Experimental Example 6: Skin irritation test of the cosmetic composition

Skin irritation of the products prepared according to the above Examples and Comparative Examples was tested on 50 healthy adults male and female. After a certain amount (approximately 0.1 g) of each sample (Examples 1-3 and Comparative Examples 1-3) was applied to the lower hind arm of each subject at 5 × 20 cm 2 for 24 hours, and then removed for 1 hour and 24 hours. The skin condition change was visually determined, and the skin irritation rate was calculated according to the following Equation 5 based on the determined result. The results are shown in Table 9.

Stimulus rate = {[(+-) number × 1 + (+) number × 2 + (++) number × 3] / (total number of subjects × 3)} × 100

Formulation Judgment result (person) Stimulation rate (%) ++ + +- - Example 1 - - 5 46 3.4 Example 2 - One 3 46 3.4 Example 3 - - 3 47 2.0 Comparative Example 1 - - 5 45 3.4 Comparative Example 2 - One 3 46 3.4 Comparative Example 3 - - 4 46 2.6 -: No erythema or unusual phenomena +-: Slightly redder than the surroundings +: Slightly redder than the surroundings ++: Severe reddening and swelling than the surroundings

As shown in Table 10, the cosmetic composition of Example 1-3 of the present invention shows a nearly similar skin irritation compared to Comparative Example 1-3 , so that the culture solution of myrcium berucaria hardly irritates the skin. I could see that. Therefore, it was found that the cosmetic composition of the present invention has less skin irritation and, therefore, excellent skin safety.

As described in detail above, the present invention provides a composition comprising a culture solution of myrcium berucaria . The culture solution of myrcium berucaria of the present invention has an improved inhibitory effect on tyrosinase activity and an excellent effect of inhibiting melanin production of melanocytes compared to the conventional whitening ingredients, and a cosmetic composition comprising the culture solution of myrosium berucaria of the present invention. The composition exhibits an excellent skin lightening effect. In addition, the culture solution of myrcium berucaria of the present invention hardly irritates the skin.

Claims (6)

Labyrinth potassium chopping Luca Ria tyrosinase activity inhibiting composition comprising the culture of (Myrothecium verrucaria) as an active ingredient. Composition for inhibiting melanin production of melanocytes comprising a culture solution of myrcium berucaria as an active ingredient. A cosmetic composition for skin whitening comprising a culture solution of mirotesium berucaria as an active ingredient. The cosmetic composition for skin whitening according to claim 3, wherein the composition inhibits tyrosinase activity and melanin production of melanocytes. The method of claim 3, wherein the content of the culture medium of the maze potassium chopping Luca Ria is a composition, characterized in that from 0.001 to 15.0 wt% based on the total weight of the composition. The group of claim 3 wherein the composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for skin whitening, characterized in that it has a formulation selected from.