KR101064934B1 - Cosmetic composition for skin whitening containing actinin derived from vitamin C and kiwi - Google Patents

Abstract

The present invention relates to a whitening cosmetic composition containing actinidine (Actinidin) derived from vitamin C and kiwi, and the actinidedine derived from vitamin C and kiwi having excellent whitening effect promotes the removal of the stratum corneum outside the skin. It relates to a cosmetic composition to enhance the skin whitening effect without irritation.

Description

Cosmetic composition comprising Vitamin C and Actinidin derived from Kiwifruit for Skin Whitening}

The present invention relates to a whitening cosmetic composition containing actinidine (Actinidin) derived from vitamin C and kiwi, and the actinidedine derived from vitamin C and kiwi having excellent whitening effect promotes the removal of the stratum corneum outside the skin. It relates to a cosmetic composition to enhance the skin whitening effect without irritation.

Human skin color is largely determined by the amount of melanin, hemoglobin, carotene and the like, of which melanin plays the most important role. Melanin is a pigment in the skin's epidermal layer that prevents skin damage caused by UV rays and protects the skin from external harmful factors such as trapping free radicals and free radicals generated in skin. .

In the process of synthesizing melanin, an enzyme called Tyrosinase, which is present in the melanocyte vesicles of Melanosome, produces dopaquinone using Tyrosine as a substrate. Quinones undergo an automatic oxidation reaction to become dopachrome. Dopachrome again produces melanin by enzymatic reaction. The melanin thus produced is transferred to the keratinocytes through the melanosomes, and after about 28 days of keratinization, the melanin comes out to the surface of the skin and disappears with the keratin.

Skin tissue synthesizes melanin to protect the body from ultraviolet rays, but melanin is promoted by various factors, and the stratum corneum is not smoothed out due to stress and skin aging. Eventually, when the aging keratin is not eliminated normally, the skin loses activity and remains on the skin, causing pigmentation such as blemishes and freckles, resulting in dark skin tone.

Therefore, in order to prevent such pigmentation phenomenon, it is necessary to inhibit the synthesis of melanin pigment or to remove the old stratum corneum. There are several mechanisms that inhibit melanin synthesis. Representative synthesis inhibition process inhibits tyrosinase, an important enzyme of melanin synthesis, and most commonly known substances include kojic acid, vitamin C, and arbutin. In order to remove aged keratin, AHA (α-hydroxy acid) is used to remove the stratum corneum of the skin or scrub and abrasives to remove keratinocytes physically through friction.

However, in order to obtain an effect by inhibiting only one process to obtain a skin whitening effect, the researches have not yet exhibited an excellent whitening effect. Therefore, in order to obtain a sufficient whitening effect, a method of inhibiting melanin biosynthesis and promoting the exfoliation of old keratin is required.

Thus, the present inventors have studied in order to enhance the skin whitening effect, when using the vitamin C and kiwi derived actinidine excellent in whitening effect, found that the whitening effect can be maximized by removing the old stratum corneum The invention was completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin whitening containing vitamin C and actinidine derived from kiwi as an active ingredient.

In order to solve the above problems, the present invention may include the following means.

The cosmetic composition for skin whitening according to the embodiment of the present invention is characterized by containing actinidine derived from vitamin C and kiwi as an active ingredient.

The vitamin C is characterized by containing 0.01 to 10.0% by weight based on the total weight of the composition.

The actinidine is characterized in that it contains 0.001 to 10.0% by weight based on the total weight of the composition.

The composition is characterized in that it has a formulation selected from the group consisting of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

The present invention provides a synergistic effect of the skin whitening effect without skin irritation by promoting the removal of the stratum corneum derived from vitamin C and kiwi having excellent whitening effect.

The cosmetic composition for skin whitening according to the present invention contains the actinin from vitamin C and kiwi as an active ingredient to further increase the skin whitening effect.

Vitamin C, an active ingredient of the present invention, has been conventionally used as a cosmetic ingredient, and has a whitening effect such as improvement of pigmentation such as blemishes, freckles, and blotch, and wrinkle improvement effect such as antioxidant and collagen biosynthesis promotion, and moisturizing effect. Known.

Actinidine, an active ingredient of the present invention, is a proteolytic enzyme extracted from kiwi, and is well known as plant proteinases such as papain, bromelain, ficin, and chemopapain. Belongs to the military. These are thiol-based proteolytic enzymes having -SH groups and are widely used in the food industry such as beer turbidity inhibitors, for meat removal, digestive agents and baking.

Actinidia chinensis used in the present invention is a subtropical plant inhabiting the Yangtze River in China, which is improved in New Zealand and cultivated worldwide. Kiwi is a deciduous plant of the genus Aceraceae. The leaves are alternate and round, round or round, or upside down. Flowers blossom in white in June-July. Fruits ripen in August-October, round egg-shaped or cylindrical, with dense brown hairs on the surface. The fruit is shaped like a kiwi bird living in New Zealand. In Korea, it was introduced in New Zealand in 1977 and is grown mainly in the south coast. On the other hand, in order to isolate and purify actinide, a protease from the kiwi used in the present invention, Cho. et al ,; Korean J. Food & Nutrition., Vol 7, No2, pp87-94, 1994.

The method for separating actinidine from the kiwi of the present invention

1) recovering the supernatant from the kiwi

2) acetone fractionation

3) eluting the active fraction by applying the fraction to chromatography

4) Check whether the eluate is purified through polyacrylamide electrophoresis

The chromatography of step 3 was subjected to ion exchange chromatography followed by gel filtration chromatography. In the preparation of the present invention, the active fraction was eluted after adding the fraction of step 2 to the ion exchange chromatography column. Next, the fraction obtained by the ion exchange chromatography was added to the gel filtration column to the column to elute the active fraction and the activity was measured. In order to confirm that the separated protease was purely separated as described above, the protease was separated and purified by performing polyacrylamide gel electrophoresis using the eluate.

On the other hand, the vitamin C is characterized in that it contains 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. When the content of the vitamin C is less than 0.01% by weight, the effect of the whitening effect is insignificant, and when the content of more than 10.0% by weight there is a risk of skin irritation.

The content of the actinidine is characterized in that it contains 0.001 ~ 10.0% by weight, when the content of the actinidine is less than 0.001% by weight keratin exfoliation promoting effect is insignificant, when the content exceeds 10.0% by weight It's hard to expect.

 The active ingredient included in the cosmetic composition of the present invention includes components conventionally used in cosmetic compositions in addition to vitamin C and actinidine, such as conventional containing such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And the like.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited to these embodiments according to the gist of the present invention to those skilled in the art to which the present invention pertains. Will be self explanatory.

Preparation Example 1 Separation and Purification of Actinidine from Kiwi

 After purchasing 1kg of Jeju Dosan Kiwi in the market, it was pulverized using a pulverizer and phosphate buffer solution (20mM phosphate buffer solution containing 5mM cystein and 2mM EDTA, pH 7.0) was added to twice the amount of the pulverized product. The homogenizer was homogenized for 5 minutes. This was centrifuged (12,000 rpm, 30 minutes) and the supernatant was used as a coenzyme.

The same amount of acetone was added to 300 ml of the crude enzyme solution and left at room temperature for 2 hours to obtain a precipitate. This was again centrifuged (4000 rpm, 10 minutes) to remove the supernatant and dried under reduced pressure to obtain acetone powder.

Acetone powder was dissolved in phosphate buffer (pH 7.0) and injected into DEAE-Toyopearl 650C column (2.5 × 28cm, Torsoh, Japan), washed with phosphate buffer (pH 7.0), and then 1ml / min with 200mM NaCl. After eluting at a rate and concentrated using ultrafiltration (Amicon Co. PM-10), the concentrated enzyme solution was injected into a Sephadex G-100 column (1.8 × 60 cm, Pharmacia LKB Biotechnology, Sweden) and phosphate buffer solution ( pH 7.0) was eluted at a rate of 9 ml / h and the activity was measured and used.

In order to analyze the activity of the protease, the eluate was recovered and measured for activity. Specifically, 0.1 ml of enzyme solution was added to 3.0 ml of a substrate solution in which casein was dissolved in a buffer solution (pH 7.0) so that the final concentration was 1.2%, and the mixture was mixed well and reacted at 35 ° C. for 10 minutes, followed by 10% trichlor acetic acid. 0.5 ml (TCA) was added and left to stand for 10 minutes to stop the reaction. The reaction solution was centrifuged (2800 rpm, 10 minutes) to measure the amount of tyrosine produced at 275 nm. At this time, one unit of protease was defined as the amount of enzyme required to generate 1 microgram (μg) of tyrosine in 1 minute.

Electrophoresis was performed on 0.15M Tris-glycine buffer solution (pH 6.8) using 7.5% polyacrylamide gel to confirm the proteolytic enzyme purification.The gel was then stained with 0.025% coomassie brilliant blue R-250 solution. It was confirmed that it is a single band.

step Protease activity (unit) DEAE-Toyopearl 650C 2450 Sephadex G-100 1987

[Production Example 2]

To prepare a cosmetic composition containing the actinidine obtained from vitamin C and kiwi in the form of a cream having a composition ratio.

ingredient Example 1
(weight%) Example 2
(weight% Example 3
(weight%) Comparative example
(weight%) Vitamin c 3.0 3.0 Actinidine 1.0 1.0 glycerin 5.0 5.0 5.0 5.0 Liquid paraffin 10.0 10.0 10.0 10.0 Polysorbate 60 2.0 2.0 2.0 2.0 Sorbitan sesquirololeate 1.0 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 5.0 Beeswax 10.0 10.0 10.0 10.0 Triethanolamine 0.5 0.5 0.5 0.5 Propylene glycol 1.0 1.0 1.0 1.0 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance system 100 100 100 100

Experimental Example 2 Skin Patch Test

In the past, skin irritation has not been shown to be irritable, and a skin test was performed on 20 women in their 20s and 30s with no skin disease or skin allergy symptoms to confirm the skin safety of the present invention. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was dropped in a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was marked for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. Skin response was determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG), and the average skin response rate was calculated according to Equation 1. The results are shown in Table 2.

[Evaluation Criteria and Score of Skin Response]

Test substance After 1 hour After 24 hours Reactivity (%)
(n = 20)  ± + ++ ± + ++ Example 1 - - - - - - 0.00 Example 2 - - - - - - 0.00 Example 3 - - - - - - 0.00 Comparative example - - - - - - 0.00

As can be seen in Table 3, it can be seen that the skin safety test is excellent because the skin irritation does not appear in the cosmetics containing vitamin C and actinidine in the skin patch test.

Experimental Example 1 Skin Whitening Effect

In order to examine the skin whitening effects of Examples 1, 2, and 3 and Comparative Example prepared in Preparation Example 1, the experiment was divided into four groups of 10 people with black skin tones in their 30-40s. After applying evenly to the entire face twice a day for 4 weeks, the brightness of the skin color was measured using a chromameter (Minolta CM-3500d). When the test substance has a whitening effect, △ L value (skin brightness after application-skin brightness before application) is increased, and the results are shown in Table 3 below.

Skin color change (△ L) 1 week 2 weeks 3 weeks 4 Weeks Example 1 1.2 2.5 3.6 5.3 Example 2 0.6 1.2 1.9 2.2 Example 3 0.4 0.8 1.4 1.7 Comparative Example 1 0.2 0.3 0.4 0.4

As can be seen in Table 4, Examples 1, 2 and 3 containing the active ingredient showed that the change in the brightness of the skin color appeared. In particular, in the case of Example 1 containing vitamin C and actinidine it was confirmed that the skin whitening effect is significantly higher than using the active ingredient alone.

Hereinafter, as an example of the cosmetic formulation of the present invention, creams, lotions, essences, packs, and body cleansers containing vitamin C and actinidine, which are the active ingredients of the present invention, are exemplified, but the formulation of the present invention is not limited thereto. It is not.

Formulation Example 1 Cream

The cream containing the said active ingredient was manufactured according to a conventional method.

Raw material name Content (% by weight) Vitamin C 3.0 Actinidine 1.0 glycerin 4.0 Vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

[Formulation Example 2] Lotion

The lotion containing the said active ingredient was manufactured according to a conventional method.

Raw material name Content (% by weight) Vitamin C 3.0 Actinidine 1.0 Cetostearyl alcohol 1.0 Glyceryl Monostearate 0.8 Sorbitan monostearate 0.3 Polysorbate 60 1.0 Mineral oil 5.0 Pyromethicone 3.0 Dimethicone 0.5 Allantoin 0.1 glycerin 5.0 Alcohol 2 Propylene glycol 3.0 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 3 Essence

Essences containing the active ingredient was prepared according to a conventional method.

Raw material name Content (% by weight) Vitamin C 3.0 Actinidine 1.0 1,3-butylene glycol 4.0 glycerin 3.0 Allantoin 0.5 Panthenol 0.1 EDTA-2Na 0.01 ethanol 5.0 Triethanolamine 1.5 Squalane 2.0 Beeswax 2.5 Polysorbate 60 3.5 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

[Formulation Example 4] Pack

The pack containing the said active ingredient was manufactured according to a conventional method.

Raw material name Content (% by weight) Vitamin C 3.0 Actinidine 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.05 Propylene glycol 5.0 glycerin 4.5 Cabopol 1.5 Polyoxide 0.2 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Formulation Example 5 Body Cleanser

A body cleanser containing the above active ingredient was prepared according to a conventional method.

Raw material name Content (% by weight) Vitamin C 3.0 Actinidine 1.0 Alkyl ether saccharide (30%) 30.0 Alkyl ether sulfosuccinate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Lauramiddiei 4.0 Cellulose gum 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount incense a very small amount antiseptic a very small amount Purified water Balance system 100

Claims (4)

A cosmetic composition for skin whitening, comprising actinidine derived from vitamin C and kiwi as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the vitamin C contains 0.01 to 10.0 wt% based on the total weight of the composition. The cosmetic composition for skin whitening according to claim 1, wherein the actinidine contains 0.001 to 10.0% by weight based on the total weight of the composition. The composition according to any one of claims 1 to 3, wherein the composition is a softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. Cosmetic composition for skin whitening, characterized in that it has a formulation selected from the group consisting of.