JP2001233725A - Cosmetic composition containing polyphenol compound derived from oenothera tetraptera - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new, safe and effective bleaching agent, active oxygen scavenger and lipoperoxide formation inhibitor and a cosmetic composition for ameliorating dry skin, eczema or skin roughening by utilizing them and giving gloss or tension to the skin or the hair on the head. SOLUTION: These bleaching agent, active oxygen scavenger and lipoperoxide formation inhibitor are obtained by including a polyphenol compound derived from Oenothera tetraptera as an active ingredient and the cosmetic composition is prepared by utilizing them.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel cosmetic effect of a garden radish polyphenol compound and its application to a cosmetic composition based thereon.

The whitening agent, active oxygen scavenger, lipid peroxide inhibitor and the cosmetic composition containing the same according to the present invention can be used to prevent pigmentation such as spots and freckles, rough skin, and rough skin. Or, it means general external preparations for the purpose of improvement. Specifically, ampules, capsules, pills, tablets, powders, granules, solids, liquids,
1) External medicines, 2) Quasi-drugs, 3) Topical or systemic skin cosmetics (for example, basic makeup such as lotions, emulsions, creams, ointments, lotions, oils, packs, etc.) Ingredients, facial cleansers and skin cleansers, massage agents,
Cleansing agent, hair remover, hair remover, shaving treatment fee, aftershave lotion, pre-show lotion,
Shaving creams, etc.), 4) Medicinal or cosmetic preparations applied to the scalp / hair (eg, shampoos, rinses, treatments, permanent solutions, hair dyes, hair styling agents, hair tonics, hair restoration / hair restoration, etc.) ), 5) Bath agent to be poured into bath water, 6) Others, axillary deodorant, deodorant, deodorant, sanitary goods, sanitary cotton, wet tissue, mouth freshener (eg, toothpaste, mouthwash, etc.) And the like.

[0003]

2. Description of the Related Art The color of human skin is determined by the amount of melanin in the epidermis,
Capillary blood flow, food-derived pigment (carotene), thickness of stratum corneum, and the like affect melanin pigment, which is reported to be the most important factor. The so-called liver spots (spots), sparrow egg spots (buckwheat), and pigmentation caused by sunlight are the ones in which the production of melanin is significantly enhanced by the activation of pigment cells existing in the skin. It is one of the big troubles.

[0004] The melanin pigment is produced by melanosomes, which are organelles in pigment cells (melanocytes) present in the basal layer of the epidermis, the hair root, and the outer root sheath. In the process of melanin production, tyrosinase is activated by tyrosine in pigment cells (melanocytes) to oxidize tyrosine,
It is converted to dopa and dopaquinone, further autoxidized, polymerized via dopachrome and 5,6-dihydroxyindole, and finally turned into a melanin pigment. The melanin thus produced is secreted from the dendrites of pigment cells (melanocytes) to basal cells, and the basal cells divide, become spiny cells and rise to reach the stratum corneum. Further, it has been reported that when this melanin is irradiated with ultraviolet rays, the existing melanin is oxidized and becomes black temporarily.

There have been many reports on methods for preventing or improving skin spots (spots), sparrow egg spots (buckwheat) and pigmentation due to pigmentation. It is said that it is effective to prevent melanin from being blackened by lightening, or to lighten the color of melanin already produced. Based on this, various whitening components have been conventionally proposed. For example, arbutin, kojic acid or a derivative thereof, ascorbic acid or a derivative thereof,
Tocopherol or a derivative thereof, glutathione, hydrogen peroxide, zinc peroxide, hydroquinone monobenzyl ether, placenta extract, silk extract and plant extract (arnica, altea, aloe, ogon, emmeiso, chamomile, licorice, gardenia, genoshoko, sicon) , Ginger, birch, senkyu, gennoshoko, psycho, sasanqua, toki, calendula, elderberry, safflower, garlic, adlay, litchi, logwood)
Etc. are known.

On the other hand, in recent years, the production of active oxygen in a living body and various effects (diseases) caused thereby have been reported. Generally the active oxygen also called ^{_{Activated oxygens, O 2 -, H}} 2 O 2, · OH, is divided into chemiluminescence, both important to participate in self-defense of biological have potent bactericidal action Is considered to be a substance. For example, when foreign enemies (antigens) such as bacteria, viruses, and foreign substances enter the living body, phagocytic neutrophils, monocytes, and macrophages in the blood first start phagocytosis, and then phagocytes. Active oxygen is produced to dissolve foreign substances phagocytosed in the endoplasmic reticulum. This active oxygen acts on the dissolution of phagocytic foods, and on the other hand, directly exerts a bactericidal action on external enemies such as bacteria and foreign substances, thereby playing a role of protecting the living body from external enemies.

However, if the active oxygen for self-defense is excessively produced and secreted, even normal cells may be lysed and stimulated, resulting in various disorders.
Recently, many diseases / diseases induced by active oxygen have been reported. For example, fats and oils such as cholesterol in blood are oxidized by active oxygen, and vascular disorders due to arteriosclerosis in the brain and heart (stroke, myocardial infarction, etc.)
And blood flow impairment due to a thrombus or the like at the time of cerebral trauma, and ischemic state (oxygen deficiency) in blood vessels due to sudden transient atrophy.

In particular, since the skin is an organ that directly contacts the outside world, it is easily affected by external environmental factors such as ultraviolet rays.
It is known that various diseases are caused by the active oxygen generated thereby. For example, if the active oxygen continues to be excessive in the skin due to ultraviolet rays or radiation,
It reacts with the unsaturated fatty acids of the phospholipids of biological membranes to generate lipid peroxides, which may cause arteriosclerosis, carcinogenesis, membrane destruction, protein degeneration, skin inflammation, and edema. Is done. Furthermore, from a cosmetic point of view as a disease caused by excess active oxygen, from the viewpoint of herpes dermatitis, x-ray dermatitis, burns, trauma, sunshine dermatitis, contact dermatitis, eczema, atopic dermatitis, etc. Instead, it induces an increase in glycosaminoglycans, cross-linking and reduction of collagen fibers, denaturation of elastic fibers, etc., causing the skin to lose its elasticity, causing external changes such as wrinkles and loosening. It is known that the phenomenon is promoted.

[0009] In order to prevent or ameliorate a disease such as the one described above, for example, superoxide dismutase (SOD), catalase, glutathione peroxidase, In addition, tocopherols (vitamin E), oryzanol, plant extracts (hamamelis, melissa, emmeiso, birch, sage, rosemary, age, basil, ginkgo, psycho, peonies, hange, cahig, tissou, ougon, carrot, licorice, ginger) Its usefulness has been pointed out.

[0010]

However, the above-mentioned whitening components or active oxygen-eliminating substances have poor stability and solubility in a prescription system, and give off odors and coloring due to sedimentation and decomposition, as well as biological substances. It was not satisfactory in practical use, for example, the effect at the level was not sufficient, and there were safety problems such as skin irritation.

[0011]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies in view of such circumstances, and as a result, a polyphenolic compound derived from the Gymnosperm has excellent usability and an effective whitening effect.
It has been found that it has an active oxygen eliminating (SOD-like) action and a lipid peroxide production inhibitory action. Further, by utilizing this, a cosmetic composition which improves dry skin, eczema, rough skin, and gives luster and tension to skin and hair has been completed.

The present invention relates to a cosmetic composition containing a whitening agent, an active oxygen scavenger and an inhibitor of lipid peroxide production, or a periwinkle-derived polyphenol compound, containing a periwinkle-derived polyphenol compound as an active ingredient.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION The "polyphenol compound derived from Gypsophila" used in the present invention is composed of flavonoid compounds such as pyrogallol tannin, galloylglucose, catechins and the like.
Including 40-80%. Further, those having a polyphenol compound content of 60% or more can be suitably used.

[0014] A plant extract obtained from the original plant of the plant, Paniculata can also be used. The term "periwinkle" used in the present invention is a plant belonging to the family Redaceae: Oenothera biennis Linne, and is generally referred to as periwinkle. In the present invention, stems, branches and leaves, leaves, bark, flowers, flower buds, fruits, pericarp, ears, seeds and the like of the plant are used, and seeds can be particularly preferably used.

A plant that has been squeezed out as it is or after crushing. Alternatively, it is extracted with a solvent as it is or after pulverization.
Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, anhydrous ethanol and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, ethyl ether, and dioxane. , Acetonitrile, and ethyl acetate can be used alone or in any combination of two or more.

The polyphenolic compound derived from Gypsophila is
Depending on the dosage form / form to be applied, dry, concentrate or dilute,
Mixing with other components can be arbitrarily prepared.

The extraction is usually carried out under normal pressure, and may be heated to increase the efficiency. After the extraction, the solution is purified by filtration and / or a column such as an ion exchange resin to obtain a solution, paste, gel, or powder. In many cases, the extract can be used as it is, but if necessary, purification treatment such as deodorization and decolorization can be performed as long as the effects of the present invention are not affected. In addition, as a purification treatment means such as deodorization and decolorization, an activated carbon column or the like can be used.

The polyphenolic compound derived from Gypsophila of the present invention can be used as a whitening agent, an active oxygen scavenger, a lipid peroxide production inhibitor, or a raw material to be incorporated into a cosmetic composition. The content varies somewhat depending on the type of the applied agent type, the method of use, and the expected degree of action, but is usually 0.001% by weight or more (hereinafter, expressed as%) as solids, and is preferably
About 0.01-20% is good.

The whitening agent, active oxygen scavenger and lipid peroxide production inhibitor or cosmetic composition according to the present invention may further comprise the following essential extracts in addition to the above-mentioned essential extracts, provided that the effects of the present invention are not impaired. Arbitrarily select the components and additives exemplified in
It can be manufactured in combination.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )Such.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oils Liquid paraffin, vaseline, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid Synthetic fatty acids such as, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) alcohols ethanol, isopropanol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, 2-hexyldecanol,
Synthetic alcohols such as isostearyl alcohol and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or its ester, trehalose or its derivative, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, alkylene such as ethylene oxide (C2-C4)
Oxide-added hydroxyalkyl (C2-C
4) chitin or chitosan, low molecular weight chitin or chitosan,
Chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or a salt thereof, hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose , Hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide or polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, Polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester nonionic surfactants, ester nonionic surfactants, block polymer nonionic surfactants, nitrogen-containing nonionic surfactants, and other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or a derivative thereof, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U Such.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decoloration, etc. You can choose to serve.

The solvent used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later, but usually, water or a water-soluble organic solvent (eg, ethanol) ,Propylene glycol,
It is preferable to use one or a mixture of two or more selected from among 1,3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Giving flexibility, relieving irritation, relieving stress by fragrance, activating cells (preventing cell aging), suppressing inflammation, improving skin and hair quality, preventing and improving rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, relief of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as fragrance, deodorization, thickening, preservation, and buffering can be expected.

In foods and drinks, there are healthy and cosmetic effects such as nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging), etc. It can be used for purposes such as thickening and preservation. Furthermore, in addition to this, we expect various cosmetic and pharmacological effects of each raw material known so far, and aim to increase the intended effect by combining them,
Alternatively, it is possible to provide a product that is expected to have a multifunctional effect.

Specific plants (crude drugs) as raw materials include:
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsujirafuji (Kibo), Acacia, Akasho (Akasho), Akasuri `` Fruit '', Red clover, Red grape, Akaminoki (Logwood), Red acacia (Red bud), Akane (Akane grass root), Red acacia or Jio ( Ground yellow), Agi (Awei), Aki Larch, Akinire (Latinaceae),
Aguay Guacu, Aguahe (Omitengu palm),
Akebi (Kidori), Asa (John Asako), Malva Asagao or Asagao (Toshibushi), Hydrangea (hydrangea), Ashitaba (Ashitaba),
Azuki (red azuki), acerola, asenyaku (Asenyaku), acira (shokuyoukanna), anise, abiu, abiurana, rape, avemaki `` fruit '', avocado, ama, amacha (sweet tea), amachaworu, amadocoro (tamatake), Amana (Kikomiko), Amaranthus (Amaranthus), Amaranthus hippochondriacus, Haribiyu, Amaranthus hippochondriax, Amigasa lily or Bimo (shellfish),
Aristolomeria (Yurizisen), Arcana (Alcanna), Algarrobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Souduq), Aloe (Length), Aloe vera, Angelica, Apricot or Hong apricot (Anjin), Ansokkou (Azusa), Yellow sapote , Ikakozorina (ground bile head), Epimedium or Epimedium
(Inyokaku), Igusa (Wicked grass), Knotweed (Tiger root),
Strawberry (first place), strawberry, fig (Fig.
Leaf)), Ichihatsu (first time), Ichibi (Fruit Aoi), Ichiyakusou (one medicinal herb), Ginkgo (Ginkgo `` seed, leaf ''), Itohimemegi (Enshi), Carob, Inu-zuna (Telelexi), Inubeyu (Sorainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwatsuchi-chi), iwahiba or iwamatsu (makikashiwa), oolong tea, wikyo (fenka), wheat (tibusanoki), ukiyagara (miyoko) ), Uguisukagura "fruit",
Himeuikyo, turmeric (Utsukin), duckweed (buoying), Usubasaishin or Keirinsaishin or Shousaisaishin (spicy), Usvenia oi, Usvenitai oi, Utsubogusa (summer dry grass), Udo or Shishiudo (Qangji, Independent, Tang Independent) ), Unyadegato (Unkaria, Cat's claw), Uba tea, Ume (Ume `` seed, pulp ''), Urajirogashi, Uwaurushi (Kusakokomomo), Unshu mandarin (Chen skin), Estragon, Ezocogi (Ezogoka), Echinashi (Hosobamurasakikirengiku) , Enishida, Enokitake (Enokitake), Ebisugusa or Cassia Tora (Keiko), Elcampre, Elderberry `` fruit '', Elemi, Elingy or Prelotus elinge, Endu (Gossip, Sophoraceae rice), Ogi or Kibananagi (yellow) Hoki), Ousei (Naruko lily, Kagi Kurumabanarukoyuri), OuHisasa Yuki or Sasanoyuki, Coptis (Coptis), Oogatahouken, giant Karasuuri (Karokon), Ooguruma (civil engineering incense), giant Tsudzurafuji
(Self defense), psyllium (vegetative child, vegetative grass), toucan hazel (haruko), toucan cactus, pygmy plant or okera
(White jujube), giant leaf sedge (banaba), giant leaf beetle "fruit", giant squirrel (grain sperm), giant caterpillar, giant hawthorn or hawthorn (yamashiko), barley
(Barley), oka, okazeri (snake bed), okra `` fruit '', ogulma (turnover), panax ginseng or ginseng (carrot), hypericum or hypericum perforatum or perforated periwinkle (brother cut grass), dragonfly (continuous cut), onamimi (blue) Ears), Onigurimi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tianma), Oniyuri or Sasayuri or Hakatayuri (Yuri), Ononis, Halibut
(Hihayu), Ominaeshi (defeated), Oyuco, Dutch mustard (Watercress), Dutch jelly, Dutch honeywort, olive "fruit, seed, leaf", oregano, orange "fruit, pericarp", silkworm (ripened yellow) , Cocoa "fruit, peel,
Seeds '', oysters (persimmons `` leaves ''), kagi kazura (crop hooks), oysters or kantosou (rensensou), gaku hydrangea, cassia, casino ki (crocodile fruit `` fruits ''), gajutsu (shadotsutsu), Kashiwa
(Sakura tree, Sakhalin), Kascarilla, Kascara sagrada, Gypsophila, Kasensou, Kanixa (Kinosato), Kaniwa, Carnation, Kanokoso (Yoshikone), Birch or Shidare kamba (White birch), Gerbera, Pumpkin, Capoknoki `` Seeds '', Kahoku Sansho (shopper), Gama (bamboo), Chamomile or Roman chamomile, Camellia japonica (weed), Camu cam (camouflage), color, Karaku Sakeman, Ragwort or Chinese Ragwort (Our melon), Ragwishak (midsummer), Ragwort, Guarana `` Seeds '', Karaho (thick), Karaya, Galu, Karin (Gourd melon), Garcinia, Kawamidori, Kawara Psycho (Seiryuna, Hakubaku), Kawazusha, Kawatake, Kawaranadeshiko (Ishitake) or Ezokawaranadeshiko ), Japanese carrot (Aoko), Japanese sagebrush (Inchinko), Kansui (Kansu), Licorice Kantarasa, Candelilla, Kanto, Canna, Raspberry (Ezo strawberry, Dutch strawberry, Shrimp strawberry, Nawashiroi strawberry, Maple strawberry, European strawberry), Kiwi `` Fruits, leaves '', Kiwiasuuri (Uroro root), Kikyo (Kikyo, Kikyo root), Kiku (Kikyo) Chrysanthemum flower, oak daisies, daffodils, kikutani daisies, catalpa (Azumizumi), daisies (sheep-of-ears), pheasants (kokomi), kizuta, kidachi aloe, lucky mint, quina, linden (cincona, red linden), quinoa (quinoa), cinnamon (yellow) ), Gymnema Sylvester,
Kimimaku, cabbage, cabbage `` immature fruit '', cucumber, gyoryu (Nishikawayanagi, Ichiyanagi), Kiraja saponaria, Kiraya, Kyransou (gold acne grass), Kumquat `` fruit '', Ginkai, Kimbusen, Kinma, Kinmizukiki
(Sengaku grass), guapeba vermelilla, guava `` fruit '', guayule, quelux infectria (gallicate), kukui nuts, wolfberry (glygi, gugiko, gugi leaf, skeletal bark), kusagi-kazura (tenmon winter), kuzu (kuzukane) ), Camphor tree, gooseberry `` fruit '', ginseng, yellowtail, gardenia, gardenia, blackberry,
Kupuas, Kumazasa, Kumatsuzura (horse whipweed), Clara (bitter butter), cranberry "fruit", chestnut "seed, fruit, astringent skin", curculigo latifolia "fruit", grapefruit "fruit / leaf", buckthorn, black stalk
(Rescue), Kurobana Hikikoshi or Hikikoshi (extended life grass), cloves (cloves, cloves), gumbinazuna (sekimei, sekimeishi), kaygai (infestation, infestation ear), celosia
(Cow flower, rooster), geccavidin, bay (laurel), kenashi sardine rose (Tsubukuryo, Yamakiko), gentiana, gennoshoko (Older's grass), kenponashi (Kigushi),
Koukisekoku, Kishumitsukan (Koji, Tachibana,
Olivenimikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana skin)), Koushinbara (Monthly flower), Kosuui mint, Kozo `` fruit '', Kocha (tea), Kohone
(River bone), kouhon (straw and kara straw), kouriang, kourikyo (takara kang), cilantro `` fruit '', koren
(Hou Lian), Scutellaria (Yellow Gon), Cowberry (Koshibachi), Coco Palm `` Fruit '', Goshuyu (Goshuyu), Goshostrawberry (Uboniko), Pepper (Hot Pepper), Cosmos, Copaiba Balsam,
Coffee `` seed, leaf '', kobushi or magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame (sesame), sesame hahagusa (gensen), goji (gomiko), Sanekazura or binankazura or Matsubusa, wheat (wheat) , Rice or rice bran “red bran, white bran”, rice oil, cola acuminata “seed”, cola vera “seed”, colony llah, fenugreek “fruit”,
Colombo, Konzulango, Kombu, Konjac, Comfrey (Shinbari grass), Sisal (Sisal Asa), Sakisima button or Sinata button or Sinensensou (Eisensen), Sakura (Oshimashima cherry, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyayamazakura, Somei Yoshino, Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura `` leaves, flowers, fruits, bark (cherry bark) ''),
Cherries, pomegranate, sasa, sasanqua, sagemodaka
(Sawashatsu), sweet potato, sugar cane, sugar beet, sapling jujube (acid jujube), saffron (safflower, west safflower), sapodilla, sapotea marillo, pomelo `` fruit '', savonso, salmon berry `` fruit '', sarasinachoma (masuma), Salvia (sage), Sawagyoukyo (Yamakosai), Sawagumi (Yamawomo), Sankaku cactus, Sangre degrad (Croton), Sansouyousou, Sanshin carrot
(37 ginseng), sanshuyu (sanshuyu), sansho (sansho),
Sandscon (Yamazone), Shea (Karite), Cyanoki `` fruit '', Shiitake (Shiitake), Shion (Shion), Dikitaris, Shikunshi (Kimiko), Perilla or Aodiso or Chirimenjiso or Kamenjiso (Shiso leaf, Shiso), Rosewood, linden,
Chinese pine, Chinese forsythia (forsythia), Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiiroshimeji,
Bunashimeji, honjimeshi, shirotamogitake), shimokuren (hot spring), shimotsukeisou, potato, peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (wheat gate winter), shukukonemisou, shukushamitsu (sunin, Shredded sand), jujudama, palm `` fruit '', ginger (ginger), giant hydrangea (Tsuneyama), shobu (iris, iris root), shojiku `` fruit '', shirakashi `` seed '',
White butterfly (seed), white clover (clover), white adzuki (chicken bone grass), white iris (small iris), white natto (flower), white nanten (minamitenmi),
Cinchona Sacrifura, Jinjuge (Mizuka, Mizuka,
Cinnamon), cinnamon, cinnamomum cassia (cinnamon bark), watermelon (watermelon), honeysuckle (gold and silver flowers, shinto winter), sorrel (acid model), swimberry "fruit", star apple, stevia, strawberry "fruit", tin shiko ,
Horsetail (Thorn), Purslane (Horse Tongue, Horse Tongue), Plum (Fruit), Pinus sylvestris (Cones), Larix sylvestris, European Lentils, European Walnut, European Hawthorn, European Dandelion, European Chestnut (Marronnier), Pear "fruit", snow pear,
Sambucus elder (Elder), Atlantic shrew (Juniper, Juniper), Achillea millefolium (Mirfoil), Atlantic rose, Aurora magnolia, Atlantic mistletoe, Western mackerel or Western mackerel, Western horseradish, Sekisho (stone irises) Cedron (Bowshukuboku), Zeniaoi, Hirohasenega, Senega, Seri, Celery, Senkyu (River Kyu), Senshinren (Shingenren), Sendan, Sennin Cactus, Senna `` Fruits, Leaves '', Senninsou (Otatate), Senbri ), Souka (grass), elephant, rosacea, buckwheat `` seed seed '', soybean potato, rhubarb (daisou), radish, soybean, daidai (orange skin, kikomi), takasaburo (dry lotus grass), takatodai (daigo), takawarabi (Inu), Dark Sweet Cherry "Fruit", Tachioi, Tachiji High-rise (time,
(Marika), Tachidokoro (Hikai), Tachiyanagi, Tamarind `` seed '', onion, tamshiba (spicy), Taranoki `` fruit, leaf, root bark '', dahlia (tenjikubotan), tarwi,
Tanshiniwa, Tanjin (Dan ginseng), Dandelion (Gankoei) or White dandelion or Moko dandelion, Danmar, Cherry `` fruit '', Tigaya `` fruit, root, bud '', Chixet carrot (Bamboo ginseng), Chicory, Chancapiedra (Kidachikomikanso) , Korean Radish (Rhuanghuang), Korean Radish (Wuyi), Korean Artemisia (Aiba), Choreimaitake (Irei), Chorogi, Tsukuritake (Mushroom), Camellia, Camellia, Tsumekusa (lacquer primrose), Camellia (Duckweed) Grass), Tsuruazuki (red azuki bean), Tsuldokudami (Hanshu crow), Tsuruna (Kankyo), Tsuru carrot (Yoshiba ginseng), Tsuwabuki, Deiko, Teuchigurumi, Duberry `` Fruit '', Tulip, Chocolate stay, Tengusa, Tencha, Tendaiyaku (Crowd medicine), touga (winter gourd), toukaizukazura,
Pepper (Pan pepper), Touki (Toki), Tokinsenka (Marigold), Tounabena (River cut), Maize or Maize hair (Nanban hair), Toad muddy (Female Sadako), Toudodo or Korean gentian (Ryubin), Tokusa (wood pirates), dokudami (jujutsu), konkon (sore root), toshishi or mamedaoshi or pear, and eucommia (tochu `` bark, leaves,
Roots), ash (qin peel), tomato, tragacanth, triacanthus `` seed '', eustoma, tormentilla, dronoki, trollooi, Niseria berry `` fruit '', potato or yam (herbal medicine), nagaikada (butcher bloom), naginata koju , Rape, rapeseed, rape bean or tatame (sword bean), jujube, jujube (daijutsu),
Naniwaibara (Kanekurako), Navena (intermittent), Nameko, Narco lily (Yellow), Peanut (peanut), Nanten (Minami Tenmi), Nambangarashi (ramie), Niosui Mire, Nigaki
(Waterwood), bitterweed (bitter mushroom), nutmeg, Kay or Nikkei or Ceylon Nikkei or Yabunikei (Cinnamon bark) or Keishi (Keishi), Chinese chive (Niriko), Elderberry
`` Flowers, stems, leaves ''), garlic (garlic), nulde (five times), leek, nemoki or neb or nebi or nemlinoki or digokubana (healing), noa thistle (daiji), eucalyptus (real fruit), nosenkazura (ryohana), nogateto , Yarrow, Sawdust (Maehu), Nobara, Nomomo, Pineapple `` Fruit '', Hibiscus (Bussouge, Furinbussouge, Roselle), Hiyindow, Hauchiwa, Pau Dosse, Hakamauraboshi (Bone Crushing), Hakusen (White Ring Skin), Huckleberry `` fruit '', chickweed (reddit), hazel (haruko), hasidorokoro (roth root), basil, lotus (lotus, lotus, lotus), parsley
(Netherland jelly), naked mussel, batata, bee or medake (bamboo boiled), patchouli, mint (lightly loaded, lightly loaded leaves), barley (yokuinin), hanasuge (chimbo), banana, hana mint, hanabishi (situri, shisuturi), vanilla bins , Papaya, japonicus (rat koji), pahalobobo, hub "whole plant, stem, leaf", paprika, hamago or honeysuckle
(Vine vine), hamasuge (katsuki), hamabishi (shikoriko), hamanas (maikai flower), hamabafu (beach storm), hamamelis, palm, rose (rose), balata, balata rubber tree,
Agaric (Agaric, Agaricus agaricus, Uskimorinosa), Haran, Pariurus (Pleurotus japonicus), Parietaria, Balsamina (Tsurureishi, Nigari), Harunire (Yellow, Yule bark, Yuba), Barbasco, Harindou, Handaikai ), Breadfruit, giant gourd (sun dried), lizard ginseng (party), pecan nuts, gangamana (stones and garlic), higuru madaria, Japanese cypress (hishomi), pistachio, beet, hitotsuba (stone reed), tsutsubayashida , Daisies (daisy), hinotaino kozuchi (cow knees), hinoki, cypress, castor, sunflower, bell pepper, himeuzu (tenoi), himega (perfume), himematsutake (kawariharatake, hiromatsutake), pimenta `` fruit '', juniper, beakyuu , Hiyu (fruit), below-flower, Hiroha okinagusa (bakuin), loquat (fruit, leaf) Stalk ", betel nut (large clothes skin, Biroko), Pu'er tea (Hiroshi? Tea), Piper Kadsura (Minamito), butterbur, coltsfoot (款冬 flower, 款冬 leaves), Eupatorium fortunei (Rankusa), hyacinth bean (扁豆), Fujimodoki
(Chowzi zakura, Kirihana), grape `` fruit, peel, seeds,
Leaf '', beech, sycamore mushroom (Cycanoderma), Brazilian liquorice, Brazilian carrot, black currant `` fruit '', blackberry, blagnen, plum `` fruit '', flucellaria, blueberry (Prunus avium), prune, floral blanka, Blond psyllium, bundou (mung beans), hazelnut, loofah, safflower (safflower), henneken, belladonna, berry `` fruit '',
Persea, Perbianberg, Pereschia grandifolia, Benisou or Ikudisa (Keiten), Boysenberry `` fruit '', Broomweed or Elderweed or Nendou or Hahakigi Kokia (Skinkoshi), Baldwink (Fengxian, Acute child, Osteophyte) , Potteria sapota, potteria lukuma, spinach, boufu (windbreak), spinach, physalis (toron), honoki (wakopoku, magnolia), bokeh (gourd), hosobaaburagiku (tokuyoku), hosobaoguruma, hosobananaokera ), Japanese beetle (Seven Seventy-seven), Chinese bean agaric, Bodaiju (Bodai tree), button (peony `` flower, leaf,
Stalks, bark ''), buttonpi (peony bark), hops, jojoba, borage (ruriticsha), bordeaux, white berries `` fruits '', hon-oniku (meat jiyo, daitei), hon-sekkoku (iron bark stone, sparrow mountain stone) , Maizulten Nanshaw (Tennan Star), Maitake (Maitake), Ephedra (Masochism), Maca, Macadamia Nuts, Margaret (Mokshungiku), Macri (Seaweed), Magwa (Mulberry White Bark, Bark, Leaves), Magnolia・ Splengeri, Masaranpouba, Masaranzuba de Ceara, Masheira de Boi, Masahua (Tamanousenhallen, Kyukonkinrenka), Matatabi (Kiten Tate), Matsukasa, Pine `` leaves, bark, roots '', Matta Oryo, Matsuhod (Bukuryo), Matico (Cordoncillo), Mayorum (Hanamint), Malvanozadin (Bittersweet), Mulberry "Fruit", Quince, Malein (Below Mow) Watermelon), Mango, Mangosteen, Mansagna (Andesca Mitsure), Mansugurumi, Mandarin `` fruit '',
Mannentake (Lingzhi), Pheasant (Kikomi `` fruits ''), Mishimasaiko (Saiko), Mizuobako or Mizuasagao (Ryugoshi),
Mizokukushi (Hanbe Lotus), Misohagi (Chisatsuna), Mizunyanagi or Shiroyanagi (Henryoku), Mitsugashiwa, Mitsuba, Midorisapote, Midorihakka, Mimosa, Myoga, Miracle Fruit `` Fruit '', Myrrh, Milobalan, Wheatwaragiku, Mukuge (Mokuge) , Mukunoki, Mukuroji (prolonged skin), Munya, Purple (purple root), Purple or purple
(Purple pearl), purple corn, purple purple (Kunming chicken blood wisteria), megakanza opuntica, mehajiki (mother grass), mebougi, melaleuca, melissa, melilot,
Melon `` fruit '', sagebrush, moso bamboo, moku giraffe, moje (koshoboku), mock berry (wood incense), maple blossom, peach (peach `` leaves, seeds, flowers, fruits ''), sprouts, morello cherry `` fruits '', Moroheiya (Burlap), yakawamurasaki potato, yakuchi (masuti), cornflower mackerel (cornflower), cornflower mint, yacon, yashabushi (arrow wheel) or himeyashabushi or giant yashabushi `` fruit,
`` Peel, ears '', willows, yatsude (octagonal gilded), mistletoe (parasitic willow), willows (kawayanagi, tachiyanagi, weeping willow, red willow, pussy willow, dogwood willow,
(Sweet willow, koreanagi, unryuyanagi, yamayamayanagi, palm, yamayanagi, giant psyllium, tyrichkinuyanagi, foxwillow, bonsai), willow `` leaf,
Stalk '', scabbard, savannah koji (purple beef), savory tobacco
(Tsurusu, Tenmei), pokeweed (commercial land), yamanoki (yamaharu), yamamomo (yangbai skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu `` fruit '', lily, yorigusa , Mugwort (Aiba), reigan (Rainuma), lime "fruit", rye, lakanka "fruit",
Raspberry `` leaves, fruits '', latania (Clameria), Rakkyo or shallots (Shiroi), lavender, longan (longan meat), agave (Aono agave,
Fukurin agave), Ryokucha (green tea), apple `` fruit, seeds, leaves, roots '', gentian, rubus or suavisimus (sweeten), litchi (lichi, lichi nucleus), lettuce (chisha), red currant `` fruit '', Red pitaya, lemon `` fruit '', lemongrass, forsythia or Chinese forsythia (forsythia), astragalus, wax bye, wax palm,
Lokon (reed, azuki), Loganberry "fruit", rosemary (mannen wax), rose hip (nobara), wasabi, cottonweed (Mengmenhua), waremoko (jiyu) and the like.

In addition, other seawaters, for example, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (such as sodium chloride, magnesium chloride, potassium chloride), sea mud or mud (fango) Species, for example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients:
Silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese,
Arsenic, water), Shotoku stone.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaonori, Susiaonori, Hiraaonori, Bouaoonori, Hosoedaoonori), Anaaousa algae (Aosa algae), and seaweed [Aosa] Kinds: Kombu (Macambu, Rishikonbu, Hosomekombu, Mitsuikonbu), Wakame, Hirome, Ao-Wakame, Giant kelp (Macrostistis pilifera, Macrocystis integrifolia, Neocitys luetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Klebanoumi prickly pear, Akaba prickly pear, Kona prickly pear, Okina prickly pear, Usuyuki prickly pear, Echinoki prickly pear], seaweed [Red algae: Hijirimen, Maxa (Tengusa), Hiracusa, Oniku , Obakusa, Kataobakusa,
Yatabegusa, Yuikiri, Shimanegusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Byaksinkirinsai, Tsunomata, Obatsunomata, Tochaka (Yahatsutsunomata), Ezotsunomata, Togetsunomata, Hirakotonoji, Kotobino, Kotobino , Hyus banori, Akamomininori] and the like.

In addition, other algae such as green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidoro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asiaticum gutta, kawashiogusa, marimo, baronia: tamagobaronia, tamabaronia, tamagata: tamagatamo, iwata: husaiwata, surichogita, heraiwata, kurokita, kurokita, kurokita Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizudzumo, Genus Genus, Genus Colecaet, Genus Genus genus, Species of the genus Cyperaceae: Species of the genus Cyperaceae, the genus of the spider genus;

Blue-green algae (Genus Lynchium: L., L., L., L .;
(Hassay, Yuremo genus, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiacacio) genus, etc.).

Brown algae (Piraella spp .: Piraella spp., Spirogyra spp.
Genus Isogawara: Isogawara, Kuroshira Genus: Gunsenkurogashira, Kashirazaki Genus: Kashirazaki, Muchimo Genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa Genus: Amidigsa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common Genus Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawazuku, genus phlebosum, genus phlebosum, genus phlebosum: genus futomozuku, genus genus moss: genus genus: genus kuromo, genus moss, genus genus Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Betula serrata, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Genus: Genus Genus: Genus Filiformis, Genus Turmo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Musicum, Genus Missiscoma, Genus L. , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombu genus: Kikuishikombu, Nejirekombu: Nekorecome genus, Kuroshimae : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-moku, Obanoki-mimoku, Genus genus:
Bulukimo, genus cyno (Kayamonoli etc.).

Red algae (Russia genus: Ushikenori, Funorinoushike, Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus, Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: rattle, rattle rattle, hirohatamatamaki genus: hirohatamaitadaki, tamamaidaki genus: tamaitadaki, kagikenori genus: kaginori, kakenenori, tengusa: himetenugosa, haitenugosa, haitenugosa, haitenugusaugou, haitenuganugou, haitenuganugou, haitenugusaugou Genus: hibiroudo, himehibiroudo,
Genus Isommedogi: Genus Isomomedo, Genus genus: Genus medusa, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekususa: Genus Nisekaregusa, Okitsubara: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattle, mushroom, shiogosagoromo, iwanokawa: ezukiiwanokawa, kainokawa: kainokawa, kaninote: kaninote, coral genus: coral, coral, mosquito mosquito , Tambanori, slippery,
Genus Isonohana: Nuracusa, Cronulla, Stinging genus, Hirakintoki: Hirakintoki, Matagawa: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Komenori, Tosakamatsu, Aedes pine, Kakraitus: Genus Odonata, Genus Larva Genus Nagaobane, Funori: Hanafunori, Fukuronori, Makuhnori, Karenikusa: Kalexigusa, Tosakamomoki: Hosobanotokamodoki, Hirohanotosakamakiki, Yatsudegatomodoka, Kurosakacamodoki, Nezashinodoka, Nezashinotokada : Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanagatakarimenia, Honooka: Hounoo, Hikagenoito: Hikagenoito, Usuginu, Nikuhono: Nikuho Noo, Benisunago genera: Benisunago, Susukakebeni genera: Susukakebeni, Okamuragusa genera:
Yamadakusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiibanodan, Kijinotho genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurushiramo, Shiramo, Ogogonori, Mizookonori, Kabanori, Tengusamodoki: Hachijotengusamodoki, Fushikrenori: Fushikenori, Namiiwatake: Namiiwatake, Spiraea:
Sponge and genus: ssp.
Genus: crocodile, crocodile, crocodile, lotus spp .: lotus spp. The genus Taogosou: Taoyanagisou, Hanasakura, Shirotsunagi: Skullconi, Shikokonori, Hananoeda: Hanaeda, Hiratagoat: Hiratagoat, Darusu: Dars, Masagoshibari, Anadars, Weedbugsa: Uebaguni, Benifukunori
Species of sarcophagi: sarcophagia, scorpion, scorpion, scorpion genus: syllabus, smelt, ssp. Family: Habutaenori, Konohanori, Suzushironori, Usbeni genus: Usbeni, Hasuginu: Hasujiginu, Nagakonohanori: Nagakonohanori, Suzuginu: Suzuginu, Abasibaginu, Hyusbanori: Kagusubanori, Jauresubanoli, Jaureusubanoli, Jaureusubanori, Jaureusubanoli
Genus Ayanishi: Genus Ayanishi, Genus Ayaginu: Genus Ayaginu, Genus Dassia: Enashidazia, Genus Shimadagi: Genus Isohagi, Genus Shimadada, Genus Dazimodoki: Genus Dazimomodoki, Genus Itogusa: Moletogusa, Genus Genus: Makuri, Yanagina, Hana, Rana Salix var. Genus Isomegusa: Isomegusa, Suginashigusa: Suginashigusa, Isobashi Genus: Isobashi, Fujimatsumo: Fujimatsumo, Sawweed: Mushroom Rihiba, Kawamozuku genera: Kawamozuku, Aokawamozuku, Himekawamozuku, Ideyukogome genera: Ideyukogome, Okichimozuku genera: Okichimozuku,
Genus genus, genus chinorimo: chinorimo, genus thysinori: chisinori).

Axle algae (Characta spp.
Hossiturimo: Hossiturimo, Lychnotanus genus, Hessian moss: Himejirasamo, Chaboshiramo, Tripera, etc., and yellow algae (Hikarimo: Hikarimo etc.).

Examples of the material derived from animal raw materials include a cockscomb extract, a cow or human placenta extract, a pig or cow extract of the stomach, duodenum or intestine or a decomposition product thereof, and a pig or cow spleen extract. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Cow milk, casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, fish meat decomposition products and the like.

(14) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice ferment extract, rice bran (red bran, white bran) ferment extract, euglena extract, raw milk or skim milk powder Lactic acid fermentation products and trehalose or derivatives thereof.

(15) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-
Aminobenzoic acid derivatives (such as paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), and methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, Octyl paramethoxycinnamate, paramethoxycinnamic acid
2-ethoxyethyl, sodium paramethoxycinnamate, potassium paramethoxycinnamate, monodiparamethoxycinnamate
Glyceryl-2-ethylhexanoate), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), urocanin Acid derivatives (such as urocanic acid and ethyl urocanate), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, Nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, diamine Kishibenzon, Okutabenzon, sulisobenzone, benzo resorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, Neo Heliopan, ESKA roll,
Zinc oxide, talc, kaolin, etc.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and so on.

(19) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (such as magnesium phosphate of vitamin C and vitamin C glucoside), hydroquinone or a derivative thereof (such as hydroquinone benzyl ether), kojic acid or a derivative thereof, vitamin E or Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (chamomile, mulberry, corn, waremokou, clara, mugwort, Honeysuckle, yellowfin, amberjack, matsuhodo, adlay, dragonflies, hops, hawthorn, eucalyptus, achillea millefolium, althea, keihi, mankeishi, hamamelis, yamaguwa, enmeisou, bellflower, toshishi, seizome, sun dried, mao, senkyu Dokkatsu, Psycho, storm, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether) and the like.

(21) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odrich, Assemble, Touki, Calendula officinalis, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley).

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, aluminum potassium sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(23) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeso, Birch, Sage, Rosemary, Minami Amami,
Ages, ginkgo, green tea) etc.

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract and the like.

(27) Antibacterial, bactericidal and disinfecting agents acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, Isopropyl methyl phenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene Octylphenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde , Hexamine, brilliant green, malachite green,
Crystal Violet, Jamal, Photosensitizer 101
No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin and the like.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Natto extract, Loofah water, Birch (birch) or Sap of Akamatsu

(29) Elastase activity inhibitor diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, sage, western elderberry, bollige, buttonpi), seaweed extract and the like.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(31) Antiandrogens Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, ginseng extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Stimulants Pepper tincture, vanillamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placenta extract, biotin, glyceride pentadecanoate and the like.

(35) Antiseborrheic agents Pyridoxine or its derivatives, sulfur, vitamin B6 and the like.

(36) Keratolytic agent Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium silicate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(38) Hair remover Strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol ,
2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, ortho Aminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-
Diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol,
Picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenyl Paraphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate,
Orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-sulfate
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy
Sodium 5-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guaiac wood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Fengqiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batuuri essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemon grass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / Colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, fresheners, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and antiallergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral preparations, deodorants / deodorants, bittering agents, seasonings, enzymes, and the like can be used, and when used in combination therewith, various additive and synergistic effects can be expected.

The whitening agent, active oxygen scavenger and lipid peroxide production inhibitor or cosmetic composition of the present invention may be in any dosage form, such as capsules, powders, granules, solids, liquids, gels,
It can be in the form of air bubbles, emulsions, creams, ointments, sheets and the like.

Specifically, for example, external preparations such as medicinal preparations, lotions, milky lotions, creams, ointments, lotions, oils, packs, etc .; , Pre-show lotion, shaving cream, shampoo, rinse,
Hair treatments, hair styling agents, perm agents, hair tonics, hair dyes, hair growth and hair growth products, etc., makeup cosmetics such as foundation, lipstick, blusher, eyeshadow, eyeliner, mascara, perfumes, bath agents , Other, toothpaste, mouth freshener / gargle, deodorant,
Various products such as deodorant, sanitary cotton, wet tissue, etc. can be used, and can be blended into general foods and drinks.

The method for adding the whitening agent, active oxygen scavenger and lipid peroxide production inhibitor of the present invention to the cosmetic composition may be added in advance or during the production. What is necessary is just to select suitably considering workability.

[0078]

The present invention will be described below with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Preparation Example 1) A 30% ethanol solution (1 kg) or 30% 1,3-butylene glycol solution of a garden plant (100 g)
(1 kg) or 30% propylene glycol solution (1 kg), or immersed in purified water (1 kg) and extracted at room temperature for 5 days and nights.
Filter the extract (dry solids: about 0.3-3.0% by weight) to about 1
kg. Further, if necessary, purification can be carried out by adsorption with an ion exchange resin and / or gel filtration. Further concentration and drying may be performed.

(Test 1) Melanin production inhibitory test "Test method and evaluation method" B16 melanoma cells were cultured for 24 hours, and this culture solution was replaced with a culture solution containing a sample. At this time, a solvent in which the sample was dissolved was added to the blank. After culturing for 3 days, the cells were treated with trypsin, and dissolved by heating in a 1N-NaOH solution containing 10% DMSO.
The absorbance at 20 nm was measured. At the same time, the MTT reduction method (Tim Mosmann; Journal of Immunological Methods p5
5-63 (1983)), the number of cells was measured, and after confirming that there was no difference in the number of surviving cells between the blank group and the sample-added group, the suppression rate (%) of the blank cells against the amount of melanin production
Was determined by Equation 1. The results are shown in FIG.

"Sample" The sample was prepared by adjusting the active ingredient of the present invention obtained in Production Example 1 so that the solid content concentration in the culture solution was 10 μg / mL, and was subjected to a test. As a comparative sample, arbutin manufactured by Sigma was prepared under the same conditions and subjected to a test.

<Equation 1> Melanin production inhibition rate (%) = ｛1-
(Sample melanin amount / blank melanin amount)｝ × 100

(Test Results) As shown in FIG. 1, it was confirmed that the production of melanin was significantly suppressed in the sample-added system, and that the active ingredient of the present invention had a high melanin production inhibitory action. Further, a stronger effect was confirmed in the sample having a higher polyphenol compound content. Although almost the same effect was recognized as arbutin, which is a known active ingredient, it was considered that the polyphenolic compound derived from Gypsophila of the present invention is useful as a whitening agent.

(Test 2) Tyrosinase activity inhibitory test "Test method and evaluation method" Tyrosine solution (L-tyrosine 0.
3 mg / mL) 1.0 mL, Macclebine buffer (pH 6.5) 1.0 mL
Was added thereto, and the mixture was heated in a thermostat at 37 ° C. for 10 minutes. 0.1 mL of a tyrosinase solution (mushroom-derived, 2500 unit / mL) was added thereto, and the mixture was further heated for 20 minutes, and the absorbance at 475 nm was measured (A). At this time, a group (a) to which purified water was added instead of the test solution was set as a blank, and a control group to which purified water was added instead of the tyrosinase solution was also used as a sample addition group (A ′) and a group to which purified water was added ( a ′). The tyrosinase activity inhibition rate was determined by Equation 2, and the results are shown in FIG. "Sample" The sample was prepared by adjusting the active ingredient of the present invention obtained in Production Example 1 to an appropriate concentration with purified water, and subjected to a test.
In addition, ascorbic acid, kojic acid, and arbutin were similarly prepared as comparative samples, and used for the test.

<Equation 2> Tyrosinase activity inhibition rate (%) =
{(A'-a)-(A'-A)} / (a'-a) × 100

[Test Results] As shown in FIG. 2, it was confirmed that the active ingredient of the present invention has a tyrosinase activity inhibitory action. In addition, when compared with known active ingredients, it was confirmed that the extract obtained in the production example of the present invention was stronger than the arbutin solution, and had almost the same tyrosinase activity inhibitory activity as kojic acid and ascorbic acid. Therefore, it is considered that the polyphenol compound derived from Gypsophila of the present invention is useful as a whitening agent.

(Test 3) Active oxygen scavenging (SOD-like activity)
Action Test In recent years, many diseases and diseases induced by active oxygen have been reported, and it has been pointed out that removal and elimination of active oxygen leads to prevention of tissue damage. In this test,
The active ingredient of the present invention obtained in Production Example 1 was evaluated for its ability to eliminate active oxygen generated by the xanthine-xanthine oxidase system using the nitro blue tetrazolium method (Lipid peroxide experiment method, p. Publishing Co., Ltd.)
It was examined by.

"Testing method and evaluation method" A 0.05 M sodium carbonate buffer (pH 10.2) containing 1.0 mM xanthine and 3.0 mM disodium ethylenediaminetetraacetate was added to 2.5 mL of a buffer solution.
Add 75 mM nitro blue tetrazolium, 0.15 W / V% bovine serum albumin, and 0.1 mL of each sample solution, and leave at 25 ° C. for 10 minutes. There is a xanthine oxidase aqueous solution 0.1m which was adjusted so that the absorbance change was about 0.3 / 20 minutes in advance.
After L was added and left at 25 ° C. for 20 minutes, the reaction was stopped with a 6 mM CuCl solution, and the absorbance at 560 nm was measured. In addition, the results obtained by determining the inhibition rate by Equation 3 using the group in which the reaction stop solution was added before adding xanthine oxidase as a blank for each sample and the control, in which the solvent in which the solvent of the sample solution was added instead of the sample were used as a control. As shown in FIG. Also 50%
The active oxygen capacity was calculated using the inhibition rate as one SOD unit.

"Sample" A sample was prepared by adjusting the active ingredient of the present invention obtained in Production Example 1 to a solid concentration of from 10 mg / mL to 0.1 mg / mL, and subjected to a test.

<Equation 3> Active oxygen scavenging rate (%) = [1- (sample
OD value-blank (sample) OD value / (control OD value-blank (control) OD value)] x 100

(Test Results) As shown in FIG. 3, the active ingredient of the present invention was observed to have an active oxygen scavenging effect, and the active ingredient had a strength of SO.
Converted to D, it was about 12.5 units / mg. Therefore, it is considered that the polyphenol compound derived from Gypsophila of the present invention is useful as an active oxygen scavenger.

(Test 4) Lipid Peroxide Production Inhibition Test Lipid peroxide is a product obtained by oxidizing unsaturated fatty acids, which may damage cell membranes in vivo, inactivate various enzymes, or denature biological proteins. And so on. As a result, it is said that various harmful effects are exerted on living tissues, such as acceleration of aging and cancerous changes. In the skin, for example, the accumulation of lipid peroxide generated by peroxidation of sebum causes changes in the cell membrane and mitochondrial functions of the skin,
It is said to not only induce symptoms such as melasma, primary irritant dermatitis, and atopic dermatitis, but also promote skin aging such as rough skin, wrinkles, and senile pigmentation. Suppression is regarded as important in dermatology. In this test, the amount of peroxide of linolenic acid generated by ultraviolet rays was measured by using the TBA method (Analytical, Biochemistry. Vol. 95, pp. 351-358 (1979)) and obtained in Production Example 1. The antioxidant effect of the active ingredient of the present invention was examined.

"Test method and evaluation method" A solution in which 0.1% linolene was dissolved in 0.8% sodium lauryl sulfate 4.
To 9 mL, add 0.1 mL of the test sample and irradiate with ultraviolet light for 1 hour. 1 mL of this reaction solution is placed in a centrifuge tube containing dibutylhydroxytoluene as a reaction terminator, 1 mL of thiobarbituric acid solution is added thereto, and the mixture is heated at 95 ° C. for 1 hour. After cooling, 4 mL of a butanol solution was added, shaken well, centrifuged, and the absorbance at 532 nm of the butanol layer was measured.
In addition, a sample to which the solvent was added instead of the sample was used as a control, and a blank which was not irradiated with ultraviolet light was set for each sample and the control, and the inhibition rate (%) of lipid peroxide production was determined by Equation 4. The results are shown in FIG. "Sample" The sample was prepared by adjusting the active ingredient of the present invention obtained in Production Example 1 so as to have a solid concentration of 0.01 mg / mL, and was subjected to a test. As a comparative sample, dl-α-tocopherol manufactured by Kishida Chemical was prepared under the same conditions and used for the test.

<Equation 4> Lipid peroxide production inhibition rate (%) =
{1- (Sample OD value-Frank (sample) OD value) / (Control OD value-Frank (control) OD value)} × 100

(Test Results) As shown in FIG. 4, the active ingredient of the present invention was found to have a stronger inhibitory effect on lipid peroxide production than the aqueous dl-α-tocopherol solution. Further, a stronger effect was confirmed in the sample having a higher polyphenol compound content. Therefore, it is considered that the polyphenolic compound derived from Gypsophila of the present invention is useful as an antioxidant.

(Test 5) Safety test (1) Primary skin irritation test The active ingredient of the present invention obtained in Production Example 1 was prepared with purified water so that the dry solid concentration was 1.0%. Was applied to the skin of shaved Japanese white rabbits (female, 3 animals per group, weight around 2.3 kg). At 24, 48, and 72 hours after application, evaluation was performed using erythema and edema as indices based on the criteria of Draize. The results confirmed that all animals did not show any erythema and edema and were safe with respect to primary skin irritation.

(Test 6) Safety test (2) Cumulative skin irritation test The active ingredient of the present invention obtained in Production Example 1 was prepared with purified water so that the dry solid concentration was 1.0%, and the back was Hartley guinea pig with female shaving (female, 3 per group, weight 330g)
0.5 mL / animal was applied to the skin before and after) once a day, 5 times a week. Application was performed over two weeks and shaving was performed on the last application day of each week. Evaluation was performed using erythema and edema as indices based on the criteria of Draize on each application day and the day after the last application day. The results confirmed that all animals did not show any erythema and edema for 2 weeks and were safe with respect to cumulative skin irritation.

(Test 7) Safety test (3) Single dose toxicity test The active ingredient of the present invention obtained in Production Example 1 was adjusted with purified water to a concentration of 12.5%, and 16 mL / kg per animal. The amount of ddy mice (male and female,
(5 animals per group, 5 weeks old) were orally administered, and the appearance and degree of toxicity symptoms were observed over time. As a result, no abnormality was observed in all the mice for 14 days, and it was confirmed that there was no abnormality in the dissection result. Therefore, the LD50 was considered to be 2,000 mg / kg or more, confirming that the substance was safe.

(Prescription Examples) Production of Various Cosmetic Compositions The following prescription examples are shown below according to the above evaluation results. Was.

(Formulation Example 1) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Soorbitan monooleate 2.0 7. 1,3-butylene glycol 1.0 8. Glycerin 2.0 9. Production example 1 5.0 10. Extract 50% 1,3-butylene glycol extract 3.0 11. Preservative (ethyl paraoxybenzoate) Appropriate amount 12. Fragrance Appropriate amount 13. Purified water 100 residue

(Formulation Example 2) Flexible lotion% by weight 1. Glycerin 5.0 2. 1,3-butylene glycol 5.0 3. Polyoxyethylene sorbitan monolaurate (20E.O) 1.0 4. Ethanol 15.0 5. Production Example 1 4.0 6. Tokinsenka 45% 1,3-butylene glycol extract 2.0 7. Vitamin C derivative 0.05 8. Arbutin 0.01 9. Antibacterial and preservative (thiamine lauryl sulfate) qs 10. Flavor qs 11. Purified water 100 Residue

(Formulation Example 3) Astringent lotion weight% 1. Sorvitol 2.0 2. POE (20) oleyl alcohol 1.0 3. Ethanol 15.0 4. Production Example 1 3.0 5. Novara 50% ethanol extract 1.0 6. Time 50% 1,3-butylene glycol extract 1.0 7. Allantoin 0.2 8. Astringent (zinc sulfocarbonate) 0.2 9. Antibacterial and preservative (thiamine lauryl sulfate) qs 10. fragrance qs 11. Purified water 100

(Formulation Example 4) Two-layer lotion (liquid / liquid type) weight% 1. squalane 8.0 2. POE sorbitan tetraoleate 0.3 3. sorbit 1.0 4. ethanol 15.0 5. Production Example 1 2.0 6. Dutch mustard 50% ethanol extract 1.0 7. Ascorbic acid 0.2 8. Astringent (sodium sulfocarbonate) 0.2 9. Preservative (phenoxyethanol) qs 10. Fragrance qs 11. Purified water 100

Formulation Example 5 Carmine Lotion (Liquid / Solid) Weight% 1. Glycerin 3.0 2. 1,3-butylene glycol 5.0 3. Ethanol 15.0 4. Iron oxide (Bengara) 0.2 5. Zinc oxide 0.5 6. Kaolin 2.0 7. Production Example 1 5.0 8. Placenta Extract 1.0 9. Camphor 0.2 10. Lactic acid or glycolic acid 0.03 11. Preservative (Photosensitizer No. 201) qs 12. fragrance qs 13. Purified water 100

(Formulation Example 6) Peel-off pack weight% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Pantothenyl ethyl ether 1.0 7. Production example 1 1.0 8. Seaweed 50% 1,3-butylene glycol extract 1.0 9. Appropriate amount of flavoring agent 10. Appropriate amount of preservative (paraoxybenzoate) 11. Purified water 100 residue

(Formulation Example 7) Cream weight% 1. Beetle wax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Olive oil 15.0 5. Borax 0.5 6. Production example 1 5.0 7. Liquid shea fat 1.0 8. Hawthorn 50 % 1,3-butylene glycol extract 2.0 9. Preservative (acrinol) 0.1 10. Appropriate perfume 11. Purified water 100

(Formulation Example 8) Face Wash Cream% by Weight 1. Myristic acid 25.0 2. Stearic acid 5.0 3. Tallow fatty acid 5.0 4. Propylene glycol 10.0 5. Potassium hydroxide 6.0 6. Coconut oil fatty acid diethanolamide 6.0 7. Production example 1 2.0 8. Honeysuckle 30% 1,3-butylene glycol extract 2.0 9. Dipotassium glycyrrhizinate 1.0 10. Lactoferrin solution 0.1 11. Preservative (salicylic acid) 0.1 12. Appropriate perfume 13. Purified water 100

(Formulation Example 9) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Production example 1 1.5 5. Loquat leaf 50% 1,3-butylene glycol extract 1.0 6. Mentha 30% ethanol extract 0.5 7. Bodaiju 45% 1,3-butylene glycol extract 0.5 8. Polyethylene powder 0.5 9. Soybean 30% ethanol extract 0.5 10. Amino acids (glycine, glutamine, etc.) 0.5 11. Preservatives (undecylenic acid, phenol) qs 12. pH adjuster qs 13. Purified water 100

(Formulation Example 10) Sunscreen cosmetics (O / W type)% by weight 1. Oxybenzone 2.0 2. Octyl paramethoxycinnamate 5.0 3. Squalane 10.0 4. Vaseline 5.0 5. Stearyl alcohol 3.0 6. Stearic acid 3.0 7. Glyceryl monostearate 2.0 8. Ethyl polyacrylate 1.0 9. 1,3-butylene glycol 6.0 10. Disodium edetate 0.1 11. Triethanolamine 99% 1.0 12. Titanium dioxide 5.0 13. Production example 1 2.5 14 Ogon 50% ethanol extract 1.0 15. Sicon 30% 1,3-butylene glycol extract 0.5 16. Preservative (paraben) qs 17. Purified water 100 residue

(Formulation Example 11) Sunscreen cosmetics (oil type)% by weight 1. Liquid paraffin 70.0 2. Ethyl p-aminobenzoate 1.0 3. Tetrahydroxybenzophenone 1.0 4. Cetyl octanoate 25.0 5. Production example 1 1.0 6. Rice germ oil 1.0 7. Barley 50% 1,3-butylene glycol extract 1.0 8. Antioxidant (butylhydroxytoluene)

(Formulation Example 12) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3. 1,3-butylene glycol 4.0 4. Lauric acid diethanolamide 2.0 5. Edetate di Sodium 0.1 6. Production Example 1 2.0 7. Roman chamomile 50% 1,3-butylene glycol extract 1.0 8. Seaweed 50% 1,3-butylene glycol extract 2.0 9.4% hydroxypropylchitosan solution 2.0 10. Antibacterial・ Preservative (thiamine lauryl sulfate) qs 11. pH adjuster qs 12. Flavors qs 13. Purified water 100 residue

(Formulation Example 13) Rinse weight% 1. Stearyl trimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Production example 1 1.0 6. Rosemary 50% 1 , 3-butylene glycol extract 1.0 7. Panax ginseng 50% ethanol extract 2.0 8. Hydrolyzed silk solution 1.0 9. Comfrey 50% ethanol extract 1.0 10. Antibacterial and preservative (benzalkonium chloride) qs 11. Fragrance Appropriate amount 12. Residue to make purified water 100

(Formulation Example 14) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Production example 1 1.0 6. Soybean hot water Extract 1.0 7. Assembly 60% ethanol extract 1.0 8. Timpi 50% 1,3-butylene glycol extract 1.0 9. Keratin oxidative degradation solution 1.0 10. pH adjuster (citric acid) qs 11. Antibacterial and preservative ( Paraben, benzalkonium chloride) Appropriate amount 12. Fragrance Appropriate amount 13. Purified water 100 residue

(Formulation Example 15) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Production Example 1 1.0 5. Seaweed 10% 1,3-butylene Glycol extract 2.0 6. Peony skin 50% ethanol extract 2.0 7. Hydroxypropyl β-1,3-glucan solution 1.0 8. Mucopolysaccharide (porcine gastric mucosa mucin) solution 1.0 9. Antibacterial and preservative (paraben) 0.1 10. Appropriate amount of fragrance 11. Residue to make purified water 100

(Formulation Example 16) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 3.0 4. Production example 1 6.0 5. Peony 50% ethanol extraction powder 1.0 6. Fermented milk extraction End 2.0

(Formulation Example 17) Coating protective agent A gauze or liniment cloth is impregnated with a prescription solution obtained by mixing an appropriate amount of an olive leaf 30% ethanol extract, antibiotics, anti-inflammatory and the like, and used as a protective agent for clothing attached to the wound. . Alternatively, Production Example 1 may be directly applied locally and covered with gauze or the like.

(Test 8) Use Effect Test A use test was conducted to evaluate the effect when the cosmetic composition of the present invention was actually used. Conditions are stains,
Sobacas, people suffering from dark and dark (34-41 years old, 8 people) and people suffering from dry skin and skin without luster (26-47 years old, 10 people)
, A paneler, an appropriate amount of the emulsion (test product) of Formulation Example 1 in the left half of the face daily after washing the face twice a day in the morning and evening, We decided to apply to half. The evaluation was performed according to the following criteria. The results are shown in Tables 1 and 2, and the numbers in the tables represent the number of persons. No one complained of skin abnormalities during the use period.

"Effect of improving spots, freckles and skin color" Effective: Stain, freckles and skin blackness became less noticeable than before (improved). Slightly effective: spots, freckles, and skin darkness were slightly reduced. Ineffective: No change from before use.

[0119]

"Effect of improving skin quality" Efficacy: The gloss and firmness of the skin increased, and the dry skin quality was improved to a crisp skin. Somewhat effective: Skin gloss and tension increased slightly, and dry skin quality was slightly improved. Ineffective: No change from before use.

[0121]

(Test Results) As shown in Tables 1 and 2, the emulsion containing the polyphenolic compound derived from the Gymnosperm of the present invention significantly improved dry skin and gave gloss and tension to the skin.
In addition, it was confirmed that it was effective in reducing spots and freckles. In addition, there was a case that it was effective in reducing eczema.

(Test 9) Use effect test A use test was carried out to evaluate the effect when the cosmetic composition of the present invention was actually used. The conditions are 14 women (31-55 years old) suffering from rough hands as panelists,
Twice daily, morning and night, apply the appropriate amount of the cream (test product) of Formulation Example 7 to one of the left and right hands, and apply the cream obtained by removing the active ingredient of the present invention from the cream to the other hand as a control product It was decided to. The evaluation was performed according to the following criteria. The results are as shown in Table 3 and the numerical values in the table represent the number of persons. still,
No one complained of skin abnormalities during the period of use.

"Surface roughness improving effect" Effective: Hand roughness was improved. Slightly effective: Hand roughening was somewhat reduced. Ineffective: No change or deterioration before use.

[0125]

(Test Results) As shown in Table 3, the cream containing the polyphenolic compound derived from the periwinkle of the present invention was:
It was confirmed that it was effective in improving or reducing hand roughness.

(Test 10) Use effect test A use test was conducted to evaluate the effect when the bath agent of the present invention was actually used. The condition is after the bath, elbow,
People suffering from knees, heels, backs, etc. (35-65
Age, 10) as panelists, and for two weeks after the start of the test,
The bath preparation of Formulation Example 16 prepared as a control product except the active ingredient of the present invention was removed, and for the next two weeks, the granule bath preparation (test product) of Formulation Example 16 was added at a rate of 30 g / bath water (200 L) ( 1 time / day). The evaluation was performed according to the following criteria. The results are shown in Table 4 and the numbers in the table represent the number of persons. still,
No one complained of skin abnormalities during the period of use.

"Improvement effect on elbows, knees, heels, and back swelling" Effective: Kasatsu was eliminated (improved). Slightly effective: Kasatsuki was slightly reduced. Ineffective: No change from before use.

[0129]

(Test 11) Use effect test A use test was conducted to evaluate the effect when the cosmetic composition of the present invention was actually used. The condition is for those who are worried that there is a lot of gloss, lack of tension, split ends and cuts (26 ~
(Age 47, 10 persons) were panelists, and for 2 weeks after the start of the test, the shampoos and rinses of Formulation Examples 12 and 13 prepared as control products were prepared by removing the active ingredient of the present invention from the shampoos and rinses.
For the next two weeks, the shampoos and rinses (test products) of Formulation Examples 12 and 13 were used every night. The evaluation was performed according to the following criteria. The results are as shown in Table 5, and the numerical values in the table represent the number of persons. No one complained of hair and scalp abnormalities during the use period.

"Hair quality improving effect" Effective: Gloss and tension of hair increased, and split ends and cut hair were improved. Slightly effective: Hair gloss and tension was slightly increased, and split ends and cut hairs were slightly improved. Ineffective: No change from before use.

[0132]

[0133]

EFFECTS OF THE INVENTION The whitening agent, active oxygen scavenger and lipid peroxide generation inhibitor or cosmetic composition containing the polyphenolic compound derived from the periwinkle of the present invention as an active ingredient have excellent melanin production inhibitory activity and tyrosinase activity inhibitory activity. It was confirmed that it had an active oxygen scavenging action and an inhibitory action on lipid peroxide production. Further, the cosmetic composition containing them has an effect of improving spots, freckles, skin color, an effect of improving dry skin, eczema, and rough skin, and a beautiful skin effect of giving gloss and tension to skin and hair, and an antiaging effect.

[Brief description of the drawings]

FIG. 1 is a view showing the results of a melanin production inhibition test of a polyphenolic compound derived from Gypsophila of the present invention.

FIG. 2 is a graph showing the results of a tyrosinase activity inhibition test of a polyphenolic compound derived from Gypsophila of the present invention.

FIG. 3 is a view showing the results of an active oxygen elimination test of a polyphenolic compound derived from Gypsophila of the present invention.

FIG. 4 is a graph showing the results of a lipid peroxide production inhibition test of a polyphenolic compound derived from Gypsophila of the present invention.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A61K 7/42 A61K 7/42 7/50 7/50 35/78 35/78 C A61P 17/16 A61P 17 / 16 F-term (reference) 4C083 AA072 AA082 AA111 AA112 AA122 AB032 AB152 AB232 AB312 AB352 AB442 AC022 AC072 AC102 AC122 AC132 AC172 AC182 AC242 AC342 AC422 AC432 AC442 AC472 AC482 AC532 AC542 AC552 AC582 AC642 AC682 AC692 AC782 AD1122 AD7923 AD412 AD442 AD452 AD512 AD532 AD642 4C088 AB12 AC01 CA04 MA63 ZA45 ZA54 ZA89

Claims (4)

[Claims] 1. A whitening agent comprising as an active ingredient a polyphenol compound derived from Gypsophila. 2. An active oxygen scavenger characterized by comprising a polyphenolic compound derived from Gypsophila as an active ingredient. 3. A lipid peroxide formation inhibitor comprising a polyphenolic compound derived from Gypsophila as an active ingredient. 4. A whitening agent according to claim 1, an active oxygen scavenger according to claim 2, and a lipid peroxide production inhibitor according to claim 3, which contain a polyphenol compound derived from Gypsophila as an active ingredient. A cosmetic composition characterized by comprising: