KR20080097985A - 간 전구체의 확대 또는 분화를 위한 세포외 매트릭스 성분 - Google Patents

간 전구체의 확대 또는 분화를 위한 세포외 매트릭스 성분 Download PDF

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KR20080097985A
KR20080097985A KR1020087014599A KR20087014599A KR20080097985A KR 20080097985 A KR20080097985 A KR 20080097985A KR 1020087014599 A KR1020087014599 A KR 1020087014599A KR 20087014599 A KR20087014599 A KR 20087014599A KR 20080097985 A KR20080097985 A KR 20080097985A
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cells
liver
collagen
cell
hepatic
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랜달 이. 맥클리랜드
로라 엠. 레이드
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유니버시티 오브 노스캐롤라이나 앳 채플 힐
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KR1020087014599A 2005-11-16 2006-11-15 간 전구체의 확대 또는 분화를 위한 세포외 매트릭스 성분 Withdrawn KR20080097985A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73687305P 2005-11-16 2005-11-16
US60/736,873 2005-11-16

Publications (1)

Publication Number Publication Date
KR20080097985A true KR20080097985A (ko) 2008-11-06

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KR1020087014599A Withdrawn KR20080097985A (ko) 2005-11-16 2006-11-15 간 전구체의 확대 또는 분화를 위한 세포외 매트릭스 성분

Country Status (13)

Country Link
US (2) US20070155009A1 (enExample)
EP (1) EP1954804A4 (enExample)
JP (1) JP2009515558A (enExample)
KR (1) KR20080097985A (enExample)
CN (1) CN101384705A (enExample)
AU (1) AU2006315163A1 (enExample)
BR (1) BRPI0618724A2 (enExample)
CA (1) CA2630355A1 (enExample)
CR (1) CR10073A (enExample)
IL (1) IL191533A0 (enExample)
RU (1) RU2008124158A (enExample)
TW (1) TW200730627A (enExample)
WO (1) WO2007059501A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180000098A (ko) * 2016-06-22 2018-01-02 한국과학기술원 접착성 헤파린과 콜라겐을 이용한 줄기세포의 배양방법

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020045260A1 (en) * 2000-10-17 2002-04-18 Shih-Chieh Hung Method of isolating mesenchymal stem cells
US8084023B2 (en) 2007-01-22 2011-12-27 The Board Of Trustees Of The University Of Arkansas Maintenance and propagation of mesenchymal stem cells
CA2680254A1 (en) * 2007-03-06 2008-09-12 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of hepatic cells
BRPI0813353A2 (pt) * 2007-06-15 2014-10-14 Univ North Carolina Sinais parácrinos de células alimentadoras mesenquimais e regulação da expansão e diferenciação de progenitores hepáticos usando os mesmos
SG183067A1 (en) * 2007-07-20 2012-08-30 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
WO2009072990A1 (en) * 2007-12-06 2009-06-11 Agency For Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
CN102282250A (zh) * 2008-05-22 2011-12-14 维斯塔治疗公司 使哺乳动物祖细胞分化为产生胰岛素的胰岛细胞的方法
US8298584B2 (en) * 2008-12-30 2012-10-30 Collagen Matrix, Inc. Biopolymeric membrane for wound protection and repair
US20100227393A1 (en) * 2009-03-06 2010-09-09 Eric Lagasse Liver stem cells: isolation of hepatic progenitor cells from the human gall bladder
CN105456292B (zh) * 2009-10-30 2019-03-26 北卡罗来纳大学教堂山分校 来自肝外胆管树的专能干细胞及其分离方法
WO2011077894A1 (ja) * 2009-12-25 2011-06-30 Scivax株式会社 スフェロイド形成促進方法およびスフェロイド形成促進培地
JPWO2011102532A1 (ja) * 2010-02-16 2013-06-17 国立大学法人九州大学 誘導肝細胞
TW201202424A (en) * 2010-05-07 2012-01-16 Univ North Carolina Method of engrafting cells from solid tissues
US9617511B2 (en) 2010-09-07 2017-04-11 The Board Of Regents Of The University Of Texas System Tissue-specific differentiation matrices and uses thereof
WO2012064606A2 (en) * 2010-11-10 2012-05-18 Wake Forest University Health Sciences Tissue-specific extracellular matrix with or without tissue protein components for cell culture
EP2917337B1 (en) * 2012-11-08 2020-01-08 InGeneron Inc. Media for culturing, preserving, and administering regenerative cells
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
CA2908225A1 (en) * 2013-04-08 2014-10-16 National Institutes Of Biomedical Innovation, Health And Nutrition Method for culturing hepatoblast-like cells and culture product thereof
WO2016010082A1 (ja) * 2014-07-16 2016-01-21 国立大学法人大阪大学 ラミニンフラグメントの細胞培養基質活性増強方法
CN108431209B (zh) * 2015-06-12 2023-02-10 新加坡科技研究局 肝脏干细胞的衍生和成熟肝细胞类型及其用途
JP6605618B2 (ja) * 2015-11-10 2019-11-20 日機装株式会社 細胞支持複合体および細胞支持複合体の製造方法
JP6755085B2 (ja) * 2015-11-10 2020-09-16 日機装株式会社 細胞支持複合体および細胞支持複合体の製造方法
RU2618989C1 (ru) * 2016-03-09 2017-05-11 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Способ лечения печеночной недостаточности
US20170369849A1 (en) * 2016-05-11 2017-12-28 The University Of North Carolina At Chapel Hill Compositions and methods for bioengineered tissues
GB2602935B (en) * 2016-07-18 2022-10-12 Harvard College Human lymphoid tissue-on-chip
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
WO2018123814A1 (ja) 2016-12-28 2018-07-05 株式会社高研 高強度コラーゲンスポンジ
CN115948331B (zh) * 2022-10-17 2024-10-22 中国人民解放军西部战区总医院 一种从小鼠胆囊中分离获取间充质干细胞的方法
WO2025244838A1 (en) * 2024-05-21 2025-11-27 Corning Incorporated Improved collagen coatings

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6069005A (en) * 1991-08-07 2000-05-30 Albert Einstein College Of Medicine Of Yeshwa University Hapatoblasts and method of isolating same
EP0597964A4 (en) * 1991-08-07 1994-11-30 Einstein Coll Med PROLIFERATION OF HEPATOCYTE PRECURSORS.
DE4222345A1 (de) * 1992-07-08 1994-01-13 Augustinus Dr Med Bader Verfahren zum Züchten einer Zellart im Kokulturverfahren mit Leberzellen
ATE414143T1 (de) * 1999-01-19 2008-11-15 Univ North Carolina Menschliche leber-vorläuferzellen
US20020187133A1 (en) * 1999-10-01 2002-12-12 Hiroshi Kubota Methods of isolating bipotent hepatic progenitor cells
US7456017B2 (en) 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
US7413897B2 (en) * 2002-03-15 2008-08-19 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
CA2680254A1 (en) * 2007-03-06 2008-09-12 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of hepatic cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180000098A (ko) * 2016-06-22 2018-01-02 한국과학기술원 접착성 헤파린과 콜라겐을 이용한 줄기세포의 배양방법

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Publication number Publication date
CA2630355A1 (en) 2007-05-24
EP1954804A4 (en) 2009-03-04
AU2006315163A1 (en) 2007-05-24
WO2007059501A2 (en) 2007-05-24
BRPI0618724A2 (pt) 2011-09-06
RU2008124158A (ru) 2010-01-10
JP2009515558A (ja) 2009-04-16
US20100093077A1 (en) 2010-04-15
EP1954804A2 (en) 2008-08-13
CR10073A (es) 2008-10-10
CN101384705A (zh) 2009-03-11
WO2007059501A3 (en) 2007-09-13
IL191533A0 (en) 2008-12-29
TW200730627A (en) 2007-08-16
US20070155009A1 (en) 2007-07-05

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Patent event date: 20080616

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