BRPI0618724A2 - componentes da matriz extracelular para expansão ou diferenciação de progenitores hepáticos - Google Patents
componentes da matriz extracelular para expansão ou diferenciação de progenitores hepáticos Download PDFInfo
- Publication number
- BRPI0618724A2 BRPI0618724A2 BRPI0618724-2A BRPI0618724A BRPI0618724A2 BR PI0618724 A2 BRPI0618724 A2 BR PI0618724A2 BR PI0618724 A BRPI0618724 A BR PI0618724A BR PI0618724 A2 BRPI0618724 A2 BR PI0618724A2
- Authority
- BR
- Brazil
- Prior art keywords
- liver
- cells
- collagen
- progenitors
- cell
- Prior art date
Links
- 210000004185 liver Anatomy 0.000 title claims abstract description 107
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 title claims abstract description 32
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 title claims abstract description 32
- 210000002744 extracellular matrix Anatomy 0.000 title claims abstract description 32
- 230000004069 differentiation Effects 0.000 title abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 57
- 210000000130 stem cell Anatomy 0.000 claims abstract description 54
- 230000002440 hepatic effect Effects 0.000 claims abstract description 20
- 238000000338 in vitro Methods 0.000 claims abstract description 14
- 230000001902 propagating effect Effects 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 151
- 210000003999 epithelial cell of bile duct Anatomy 0.000 claims description 83
- 108010085895 Laminin Proteins 0.000 claims description 29
- 102000007547 Laminin Human genes 0.000 claims description 29
- 210000001519 tissue Anatomy 0.000 claims description 28
- 230000001605 fetal effect Effects 0.000 claims description 21
- 102000001187 Collagen Type III Human genes 0.000 claims description 19
- 108010069502 Collagen Type III Proteins 0.000 claims description 19
- 108010067306 Fibronectins Proteins 0.000 claims description 14
- 102000016359 Fibronectins Human genes 0.000 claims description 14
- 230000001010 compromised effect Effects 0.000 claims description 12
- 102000004266 Collagen Type IV Human genes 0.000 claims description 11
- 108010042086 Collagen Type IV Proteins 0.000 claims description 11
- 229920002971 Heparan sulfate Polymers 0.000 claims description 7
- 210000000648 angioblast Anatomy 0.000 claims description 7
- 229920002674 hyaluronan Polymers 0.000 claims description 7
- 229920002683 Glycosaminoglycan Polymers 0.000 claims description 5
- 239000002243 precursor Substances 0.000 claims description 5
- 102000016942 Elastin Human genes 0.000 claims description 4
- 108010014258 Elastin Proteins 0.000 claims description 4
- 229920002549 elastin Polymers 0.000 claims description 4
- 239000004017 serum-free culture medium Substances 0.000 claims description 4
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims description 3
- 229920001287 Chondroitin sulfate Polymers 0.000 claims description 3
- 241001529936 Murinae Species 0.000 claims description 3
- 229940059329 chondroitin sulfate Drugs 0.000 claims description 3
- 239000002198 insoluble material Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 229920000045 Dermatan sulfate Polymers 0.000 claims description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 2
- AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 claims description 2
- 229940051593 dermatan sulfate Drugs 0.000 claims description 2
- 229920000669 heparin Polymers 0.000 claims description 2
- 229960002897 heparin Drugs 0.000 claims description 2
- KIUKXJAPPMFGSW-MNSSHETKSA-N hyaluronan Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-MNSSHETKSA-N 0.000 claims 1
- 229940099552 hyaluronan Drugs 0.000 claims 1
- 102000008186 Collagen Human genes 0.000 description 55
- 108010035532 Collagen Proteins 0.000 description 55
- 229920001436 collagen Polymers 0.000 description 55
- 210000003897 hepatic stem cell Anatomy 0.000 description 37
- 239000011159 matrix material Substances 0.000 description 35
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 27
- 102000012422 Collagen Type I Human genes 0.000 description 26
- 108010022452 Collagen Type I Proteins 0.000 description 26
- 239000004202 carbamide Substances 0.000 description 24
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 22
- 239000000758 substrate Substances 0.000 description 20
- 210000005229 liver cell Anatomy 0.000 description 19
- 230000000694 effects Effects 0.000 description 18
- 238000001000 micrograph Methods 0.000 description 15
- 210000004738 parenchymal cell Anatomy 0.000 description 14
- 210000003995 blood forming stem cell Anatomy 0.000 description 13
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 description 12
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 description 12
- 230000014509 gene expression Effects 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- 239000010410 layer Substances 0.000 description 11
- 102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 description 10
- 108010066302 Keratin-19 Proteins 0.000 description 10
- 229910021529 ammonia Inorganic materials 0.000 description 10
- 108010088751 Albumins Proteins 0.000 description 9
- 102000009027 Albumins Human genes 0.000 description 9
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 9
- 108010069196 Neural Cell Adhesion Molecules Proteins 0.000 description 9
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 description 9
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 9
- 210000003743 erythrocyte Anatomy 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 230000004044 response Effects 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 210000003494 hepatocyte Anatomy 0.000 description 8
- 230000035755 proliferation Effects 0.000 description 8
- 230000027455 binding Effects 0.000 description 7
- 239000006285 cell suspension Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 210000000603 stem cell niche Anatomy 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 230000000890 antigenic effect Effects 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 5
- 238000011835 investigation Methods 0.000 description 5
- 210000005228 liver tissue Anatomy 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 239000012679 serum free medium Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 229920001917 Ficoll Polymers 0.000 description 4
- 108010067787 Proteoglycans Proteins 0.000 description 4
- 102000016611 Proteoglycans Human genes 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000024245 cell differentiation Effects 0.000 description 4
- 230000032823 cell division Effects 0.000 description 4
- 230000005757 colony formation Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 210000000267 erythroid cell Anatomy 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 101150079978 AGRN gene Proteins 0.000 description 3
- 101710149366 Afamin Proteins 0.000 description 3
- 102100040026 Agrin Human genes 0.000 description 3
- 108700019743 Agrin Proteins 0.000 description 3
- 102100036597 Basement membrane-specific heparan sulfate proteoglycan core protein Human genes 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 241000252203 Clupea harengus Species 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 102000008055 Heparan Sulfate Proteoglycans Human genes 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108090000054 Syndecan-2 Proteins 0.000 description 3
- 108010017842 Telomerase Proteins 0.000 description 3
- 108010006886 Vitrogen Proteins 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 238000002659 cell therapy Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 229940096422 collagen type i Drugs 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 210000003038 endothelium Anatomy 0.000 description 3
- 230000006862 enzymatic digestion Effects 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 230000002055 immunohistochemical effect Effects 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 108010049224 perlecan Proteins 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 206010037844 rash Diseases 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000011534 wash buffer Substances 0.000 description 3
- 102100031585 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Human genes 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 101100480809 Arabidopsis thaliana TCP10 gene Proteins 0.000 description 2
- 101100206196 Arabidopsis thaliana TCP3 gene Proteins 0.000 description 2
- -1 BoTr Polymers 0.000 description 2
- 108091016585 CD44 antigen Proteins 0.000 description 2
- 102000000905 Cadherin Human genes 0.000 description 2
- 108050007957 Cadherin Proteins 0.000 description 2
- 102000005598 Chondroitin Sulfate Proteoglycans Human genes 0.000 description 2
- 108010059480 Chondroitin Sulfate Proteoglycans Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102100025682 Dystroglycan 1 Human genes 0.000 description 2
- 108010071885 Dystroglycans Proteins 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101000777636 Homo sapiens ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Proteins 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101000599852 Homo sapiens Intercellular adhesion molecule 1 Proteins 0.000 description 2
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 2
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 2
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- 101100260060 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) CCT3 gene Proteins 0.000 description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000011748 cell maturation Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000036755 cellular response Effects 0.000 description 2
- 230000003021 clonogenic effect Effects 0.000 description 2
- 239000000512 collagen gel Substances 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005755 formation reaction Methods 0.000 description 2
- 235000021588 free fatty acids Nutrition 0.000 description 2
- 238000001879 gelation Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000003394 haemopoietic effect Effects 0.000 description 2
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 108010008217 nidogen Proteins 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 229910052711 selenium Inorganic materials 0.000 description 2
- 239000011669 selenium Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 102100031940 Epithelial cell adhesion molecule Human genes 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 102100035716 Glycophorin-A Human genes 0.000 description 1
- 108091005250 Glycophorins Proteins 0.000 description 1
- 102000015779 HDL Lipoproteins Human genes 0.000 description 1
- 108010010234 HDL Lipoproteins Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 101000920667 Homo sapiens Epithelial cell adhesion molecule Proteins 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 206010053159 Organ failure Diseases 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 102100033237 Pro-epidermal growth factor Human genes 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 101100068077 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN2 gene Proteins 0.000 description 1
- 241000519995 Stachys sylvatica Species 0.000 description 1
- 102000019361 Syndecan Human genes 0.000 description 1
- 108050006774 Syndecan Proteins 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 210000000013 bile duct Anatomy 0.000 description 1
- 230000008512 biological response Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008619 cell matrix interaction Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 230000000112 colonic effect Effects 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- FSEUPUDHEBLWJY-HWKANZROSA-N diacetylmonoxime Chemical compound CC(=O)C(\C)=N\O FSEUPUDHEBLWJY-HWKANZROSA-N 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012997 ficoll-paque Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 108010045676 holotransferrin Proteins 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 229960000890 hydrocortisone Drugs 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 108010038862 laminin 10 Proteins 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000003061 neural cell Anatomy 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000005691 oxidative coupling reaction Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- XNSAINXGIQZQOO-SRVKXCTJSA-N protirelin Chemical compound NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H]1NC(=O)CC1)CC1=CN=CN1 XNSAINXGIQZQOO-SRVKXCTJSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000000276 sedentary effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000004500 stellate cell Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/03—Coculture with; Conditioned medium produced by non-embryonic pluripotent stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US73687305P | 2005-11-16 | 2005-11-16 | |
| US60/736,873 | 2005-11-16 | ||
| PCT/US2006/060904 WO2007059501A2 (en) | 2005-11-16 | 2006-11-15 | Extracellular matrix components for expansion or differentiation of hepatic progenitors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0618724A2 true BRPI0618724A2 (pt) | 2011-09-06 |
Family
ID=38049393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0618724-2A BRPI0618724A2 (pt) | 2005-11-16 | 2006-11-15 | componentes da matriz extracelular para expansão ou diferenciação de progenitores hepáticos |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20070155009A1 (enExample) |
| EP (1) | EP1954804A4 (enExample) |
| JP (1) | JP2009515558A (enExample) |
| KR (1) | KR20080097985A (enExample) |
| CN (1) | CN101384705A (enExample) |
| AU (1) | AU2006315163A1 (enExample) |
| BR (1) | BRPI0618724A2 (enExample) |
| CA (1) | CA2630355A1 (enExample) |
| CR (1) | CR10073A (enExample) |
| IL (1) | IL191533A0 (enExample) |
| RU (1) | RU2008124158A (enExample) |
| TW (1) | TW200730627A (enExample) |
| WO (1) | WO2007059501A2 (enExample) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020045260A1 (en) * | 2000-10-17 | 2002-04-18 | Shih-Chieh Hung | Method of isolating mesenchymal stem cells |
| US8084023B2 (en) | 2007-01-22 | 2011-12-27 | The Board Of Trustees Of The University Of Arkansas | Maintenance and propagation of mesenchymal stem cells |
| CN101855340A (zh) * | 2007-03-06 | 2010-10-06 | 北卡罗来纳大学查珀尔希尔分校 | 用于细胞维持、扩增和/或分化的透明质酸、其他基质成分、激素和生长因子的复合物 |
| TW200916581A (en) * | 2007-06-15 | 2009-04-16 | Univ North Carolina | Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same |
| CA2693481A1 (en) * | 2007-07-20 | 2009-01-29 | Cellartis Ab | A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells |
| WO2009072990A1 (en) * | 2007-12-06 | 2009-06-11 | Agency For Science, Technology And Research | Method for extracellular matrix mediated differentiation and proliferation of stem cells |
| US8877497B2 (en) * | 2008-05-22 | 2014-11-04 | Vesta Therapeutics Inc. | Method of differentiating mammalian progenitor cells into insulin producing pancreatic islet cells |
| US8298584B2 (en) * | 2008-12-30 | 2012-10-30 | Collagen Matrix, Inc. | Biopolymeric membrane for wound protection and repair |
| US20100227393A1 (en) * | 2009-03-06 | 2010-09-09 | Eric Lagasse | Liver stem cells: isolation of hepatic progenitor cells from the human gall bladder |
| ES2819028T3 (es) * | 2009-10-30 | 2021-04-14 | Univ North Carolina Chapel Hill | Células madre multipotentes del árbol biliar extrahepático y métodos para su aislamiento |
| WO2011077894A1 (ja) * | 2009-12-25 | 2011-06-30 | Ｓｃｉｖａｘ株式会社 | スフェロイド形成促進方法およびスフェロイド形成促進培地 |
| WO2011102532A1 (ja) * | 2010-02-16 | 2011-08-25 | 国立大学法人九州大学 | 誘導肝細胞 |
| TW201202424A (en) * | 2010-05-07 | 2012-01-16 | Univ North Carolina | Method of engrafting cells from solid tissues |
| AU2011299327B2 (en) * | 2010-09-07 | 2015-07-16 | The Board Of Regents Of The University Of Texas System | Tissue-specific differentiation matrices and uses thereof |
| US9938502B2 (en) | 2010-11-10 | 2018-04-10 | Wake Forest University Health Sciences | Tissue-specific extracellular matrix with or without tissue protein components for cell culture |
| BR112015010256A2 (pt) * | 2012-11-08 | 2017-07-11 | Arthrodynamic Tech Animal Health Division Inc | meio para cultivar, preservar e administrar células regenerativas |
| US9533013B2 (en) | 2013-03-13 | 2017-01-03 | University Of North Carolina At Chapel Hill | Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree |
| US20160046904A1 (en) * | 2013-04-08 | 2016-02-18 | National Institutes Of Biomedical Innovation, Health And Nutrition | Method For Culturing Hepatoblast-Like Cells And Culture Product Thereof |
| CN106559994A (zh) * | 2014-07-16 | 2017-04-05 | 国立大学法人大阪大学 | 层粘连蛋白片段的细胞培养基质活性增强方法 |
| US10683484B2 (en) * | 2015-06-12 | 2020-06-16 | National University Of Singapore | Derivation of hepatic stem cells and mature liver cell types and uses thereof |
| JP6605618B2 (ja) * | 2015-11-10 | 2019-11-20 | 日機装株式会社 | 細胞支持複合体および細胞支持複合体の製造方法 |
| JP6755085B2 (ja) * | 2015-11-10 | 2020-09-16 | 日機装株式会社 | 細胞支持複合体および細胞支持複合体の製造方法 |
| RU2618989C1 (ru) * | 2016-03-09 | 2017-05-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Способ лечения печеночной недостаточности |
| US20170369849A1 (en) * | 2016-05-11 | 2017-12-28 | The University Of North Carolina At Chapel Hill | Compositions and methods for bioengineered tissues |
| KR101895669B1 (ko) * | 2016-06-22 | 2018-09-05 | 한국과학기술원 | 접착성 헤파린과 콜라겐을 이용한 줄기세포의 배양방법 |
| GB2569463A (en) | 2016-07-18 | 2019-06-19 | Harvard College | Human lymphoid tissue-on-chip |
| CN106434532A (zh) * | 2016-12-22 | 2017-02-22 | 叶宗耀 | 一种培养肝细胞的培养基及其制备方法 |
| US11559603B2 (en) * | 2016-12-28 | 2023-01-24 | Koken Co., Ltd. | High-strength collagen sponge |
| CN115948331B (zh) * | 2022-10-17 | 2024-10-22 | 中国人民解放军西部战区总医院 | 一种从小鼠胆囊中分离获取间充质干细胞的方法 |
| WO2025244838A1 (en) * | 2024-05-21 | 2025-11-27 | Corning Incorporated | Improved collagen coatings |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2115140A1 (en) * | 1991-08-07 | 1993-02-18 | Lola M. Reid | Proliferation of hepatocyte precursors |
| US6069005A (en) * | 1991-08-07 | 2000-05-30 | Albert Einstein College Of Medicine Of Yeshwa University | Hapatoblasts and method of isolating same |
| DE4222345A1 (de) * | 1992-07-08 | 1994-01-13 | Augustinus Dr Med Bader | Verfahren zum Züchten einer Zellart im Kokulturverfahren mit Leberzellen |
| CA2877233A1 (en) | 1999-01-19 | 2000-07-27 | Lola M. Reid | Human liver progenitors |
| US20020187133A1 (en) * | 1999-10-01 | 2002-12-12 | Hiroshi Kubota | Methods of isolating bipotent hepatic progenitor cells |
| US7456017B2 (en) * | 1999-10-01 | 2008-11-25 | University Of North Carolina At Chapel Hill | Processes for clonal growth of hepatic progenitor cells |
| HUE032238T2 (en) * | 2002-03-15 | 2017-09-28 | Univ North Carolina Chapel Hill | Primitive and proximal liver stem cells |
| CN101855340A (zh) * | 2007-03-06 | 2010-10-06 | 北卡罗来纳大学查珀尔希尔分校 | 用于细胞维持、扩增和/或分化的透明质酸、其他基质成分、激素和生长因子的复合物 |
-
2006
- 2006-11-15 RU RU2008124158/13A patent/RU2008124158A/ru not_active Application Discontinuation
- 2006-11-15 WO PCT/US2006/060904 patent/WO2007059501A2/en not_active Ceased
- 2006-11-15 CA CA002630355A patent/CA2630355A1/en not_active Abandoned
- 2006-11-15 CN CNA2006800509212A patent/CN101384705A/zh active Pending
- 2006-11-15 BR BRPI0618724-2A patent/BRPI0618724A2/pt not_active IP Right Cessation
- 2006-11-15 EP EP06839887A patent/EP1954804A4/en not_active Withdrawn
- 2006-11-15 JP JP2008541461A patent/JP2009515558A/ja not_active Withdrawn
- 2006-11-15 AU AU2006315163A patent/AU2006315163A1/en not_active Abandoned
- 2006-11-15 KR KR1020087014599A patent/KR20080097985A/ko not_active Withdrawn
- 2006-11-15 US US11/560,049 patent/US20070155009A1/en not_active Abandoned
- 2006-11-16 TW TW095142412A patent/TW200730627A/zh unknown
-
2008
- 2008-05-18 IL IL191533A patent/IL191533A0/en unknown
- 2008-06-16 CR CR10073A patent/CR10073A/es not_active Application Discontinuation
-
2009
- 2009-10-02 US US12/572,711 patent/US20100093077A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| IL191533A0 (en) | 2008-12-29 |
| CN101384705A (zh) | 2009-03-11 |
| CR10073A (es) | 2008-10-10 |
| US20070155009A1 (en) | 2007-07-05 |
| EP1954804A2 (en) | 2008-08-13 |
| JP2009515558A (ja) | 2009-04-16 |
| US20100093077A1 (en) | 2010-04-15 |
| KR20080097985A (ko) | 2008-11-06 |
| TW200730627A (en) | 2007-08-16 |
| CA2630355A1 (en) | 2007-05-24 |
| RU2008124158A (ru) | 2010-01-10 |
| EP1954804A4 (en) | 2009-03-04 |
| WO2007059501A3 (en) | 2007-09-13 |
| AU2006315163A1 (en) | 2007-05-24 |
| WO2007059501A2 (en) | 2007-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20100093077A1 (en) | Extracellular matrix components for expansion or differentiation of hepatic progenitors | |
| KR101119878B1 (ko) | 프리미티브 간 줄기세포 및 프록시멀 간 줄기세포 | |
| Irons et al. | Three-dimensional neural constructs: a novel platform for neurophysiological investigation | |
| McClelland et al. | Gradients in the liver's extracellular matrix chemistry from periportal to pericentral zones: influence on human hepatic progenitors | |
| US10870834B2 (en) | Isolated populations of adult renal cells and methods of isolating and using same | |
| Newland et al. | Macroporous heparin-based microcarriers allow long-term 3D culture and differentiation of neural precursor cells | |
| US20240368508A1 (en) | Stackable plates for culturing tissue models | |
| Salerno et al. | Membrane bioreactor for expansion and differentiation of embryonic liver cells | |
| Sirko et al. | Structural and functional analysis of chondroitin sulfate proteoglycans in the neural stem cell niche | |
| Pietryga et al. | Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration | |
| Lu et al. | The effect of dimensionality on growth and differentiation of neural progenitors from different regions of fetal rat brain in vitro: 3-dimensional spheroid versus 2-dimensional monolayer culture | |
| US20080026463A1 (en) | Matrix and method for isolation of hepatic progenitor cells | |
| Suh et al. | Behavior of isolated rat oval cells in porous collagen scaffold | |
| MX2008006456A (en) | Extracellular matrix components for expansion or differentiation of hepatic progenitors | |
| HK1225070A (en) | Primitive and proximal hepatic stem cells | |
| Gupta | Parameter optimisation for expansion of pluripotent stem cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: CONFORME ARTIGO 10O DA RESOLUCAO 124/06, CABE SER ARQUIVADO REFERENTE AO NAO RECOLHIMENTO DA 5A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2129 DE 25/10/2011. |
|
| B15K | Others concerning applications: alteration of classification |
Ipc: C12N 5/071 (2010.01) |