KR20050072303A - Body hair growth inhibitor - Google Patents

Abstract

The present invention relates to a hair growth inhibitor, comprising as an active ingredient for inhibiting the growth or development of hair, the extract of Gobon and / or Schisandra chinensis excellent in inhibiting the testosterone 5α-reductase activity and inhibiting the growth phase conversion of the hair Since the hair growth inhibitory composition has an excellent hair growth inhibitory effect, it can be applied not only to products aimed at hair loss and depilation, but also to products aimed at effective management of hair after hair loss and depilation.

Description

Hair Growth Inhibitor {BODY HAIR GROWTH INHIBITOR}

The present invention relates to a hair growth inhibitor, and in particular, as an active ingredient for inhibiting the growth or development of hair, extracts of Gobon and / or Schizandra chinensis, which are excellent in inhibiting testosterone 5α-reductase activity and inhibiting hair growth transition, It relates to a hair growth inhibitory composition comprising.

Hair and hair have functioned as one of the body's important defense means to protect the organs of the body from external stimuli. Now, except for the hair, the hair and body function as a defense means has become meaningless.

In particular, from an aesthetic point of view, it is preferable that a woman have no hair on the contrary, and accordingly, various methods are used to remove the hair. Hair removal methods include the use of mechanical means, such as an electric shaver, hair removal machine, ultrasound, laser, etc., to remove or cut hair, or by using physical means such as adhesive tape, and chemical removal of hair removal cream, wax, gel, etc. There are various methods, such as a method of performing hair loss using a means, and it has been reported that a preparation containing a specific protease is used as a permanent hair loss agent.

However, the hair loss method of pulling out body hair using mechanical or physical means involves pain, and conventional chemical removal of hair removal creams or waxes may cause pain and damage to skin caused by chemical components. In addition, hair growth is still insufficient and lacks in sustainability, and according to the growth of hair, there is a disadvantage in that hair removal treatment must be performed after a certain period of time.

In order to overcome the disadvantages of such chemical means, female hormones such as estradiol, estrone, ethynyl estradiol, etc., which can alleviate the action of male hormones for the purpose of inhibiting hair growth, are formulated as a predominant agent. It is known to be inadequate.

An object of the present invention is to provide a hair growth inhibitor composition that can safely inhibit the growth and development of hair, while being safe for the human body in view of the problems of the conventional hair removal agent by chemical means.

In order to achieve the above object, the present invention provides a hair growth inhibitory composition comprising at least one of the main extract and Schizandra extract as an active ingredient.

Here, it is preferable that the extract and Schizandra chinensis extract are 0.01 to 10% by weight based on the total weight of the composition, respectively.

As a method of directly relieving the activity of androgenic hormone and inhibiting hair growth, the present inventors noted that the action of testosterone 5α-reductase enzyme is inhibited. Testosterone 5α-reductase is an enzyme found in the prostate, the dermis, sebaceous glands, and hair follicles. It acts to convert testosterone, a male hormone, to dihydrotestosterone (DHT), an active male hormone. It is known that dihydrotestosterone, when acting on the scalp, inhibits the growth of the hair and induces alopecia areata, but when it acts on other body parts, it has been known to have the opposite effect of promoting the growth of hair, such as the hair of the beard or armpits. Therefore, it is an object of the present invention that inhibiting the action of testosterone 5α-reductase to inhibit the production of dihydrotestosterone in tissues can effectively inhibit the growth and development of body hair.

Accordingly, the present inventors have selected testosterone 5α-reducta by selecting dozens of substances which have a safety for the human body for the purpose of developing a substance having an effect of effectively inhibiting hair growth and reducing the number of hair removal treatments. Studies on substances that inhibit the action of the enzyme and inhibit the growth of the hair growth stage of the hair, the extract of Gobon or Schisandra chinensis showed the inhibitory activity of the potent testosterone 5α-reductase enzyme and inhibitor of hair growth stage It was confirmed to complete the present invention.

That is, the composition of the present invention contains, as an active ingredient, an extract of Gobon and / or Schizandra chinensis, which is excellent in inhibiting testosterone 5α-reductase activity activity and inhibiting growth phase conversion of body hair, preferably 0.01 to 10% by weight. do.

In the composition of the present invention, the extract of Gobon (Angel tenuissima ) or other herbal plants (Amaceae, Umbelliferae) using the root of the herbal medicine, the herbal name is Angelicae tenuissimae Radix. The root stem contains about 0.5% essential oil, the main components are butylidene phthalide senkyunolid, ligustilide, and seeds are β-sitosterol and francola. Prangolarin and the like are contained. For medicinal use, it has been used in toothaches to combat the movement system, dermatology and gynecological diseases.

In addition, in the composition of the present invention, Schisandra chinensis extract is an extract of a herbal medicine using a fruit of Schisandra chinensis ( schizandra chinensis ) or other related plants (Schizondraceae, Schizandraceae). Ingredients include: schizandrin, gomisin AQ, citral, β-ylangene, citric acid, malic acid, β-cami Green (β-chamigrene), fatty oil (fatty oil) and the like.

Gobon extract or Schisandra chinensis extract, the active ingredient of the hair growth inhibitory composition according to the present invention, can be obtained from any part of the raw material of herbal medicines or leaves, roots, fruits, seeds, flowers.

The preparation method of Gobon extract and Schisandra chinensis extract of the present invention composition is as follows:

First, extracts of several to tens of times the weight of the extract are added to the dried herbal medicine, extracted by chilling for 5 to 7 days, or extracted by heating for several hours by filtration, concentration, and freeze drying. Drying to obtain dried powder of Gobon extract and Schisandra chinensis extract. The yield is about 1-10% for Kobon extract and about 5-25% for Schisandra chinensis extract.

Extraction solvents used for the preparation of the extract and Schizandra chinensis extract of the present invention include purified water; Alcohols such as methanol, ethanol, propanol and butanol; Polyhydric alcohols such as glycerol, propylene glycol and 1,3-butylene glycol; Ketones such as acetone and methyl ethyl ketone; Esters such as methyl acetate and ethyl acetate; Linear or cyclic ethers such as tetrahydrofuran and diethyl ether; Halogenated hydrocarbons such as dichloromethane, chloroform and carbon tetrachloride; Hydrocarbons such as hexane, cyclohexane and petroleum ether; Aromatic hydrocarbons such as benzene and toluene; Polyethers such as polyetherglycol; It is preferable to mix one or two or more solvents from solvents such as pyridines.

The composition according to the present invention is not only a product aimed at hair loss or depilation, but also a hair loss agent, shampoo, hair conditioner, hair tonic, hair styling gel, hair loss wax, shaving product, It can be applied to one or more kinds of products such as depilatory agent, after shaving product, deodorant, deodorant, antiperspirant and bathing agent.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative of the present invention, and the scope of the present invention is not limited to these.

Preparation Example: Preparation of Hair Growth Inhibitor Composition

(1) Example of Preparation of Bonbon Extract

After 300 g of commercially available Angelicae tenuissimae Radix was purchased and chopped, about 3 kg of ethanol was added thereto, and the extracted crude solution obtained by cooling for 5 days was filtered, concentrated, and lyophilized to obtain 13.5 g of a dry powder of the ancient bone extract. Yield: 4.5%)

(2) Example of Schizandra chinensis Extract Preparation

300 g of Schizandrae Fructus, commercially available, was washed, and then made into a powder using a grinder. The extract was obtained by adding ethanol (about 3 kg) and chilling for 5 days. 54 g were obtained. (Yield: 18%)

Effect Test Example 1 Evaluation of Testosterone 5α-Reductase Enzyme Inhibitory Activity

Testosterone 5α-reductase used in the enzyme activity inhibition experiment was obtained from rat liver (adult, Sprague-Dawley, mature male rats (8-10 weeks old)), and a certain amount of protein suspension was prepared by protein quantification. In addition, an enzyme reaction solution was prepared by a general method, and radiolabeled testosterone ( ³ H testosterone) was used as a reaction substrate of testosterone 5α-reductase.

The reaction was carried out in an incubator for 2 minutes at a ratio of enzyme solution: protein suspension: bone or Schizandrae extract = 70: 20: 10, and then the reaction solvent was blown off, and the remaining material was dissolved in a developing solvent and developed on a thin layer chromatography (TLC). The amount of testosterone developed and the amount of dihydrotestosterone were quantitatively analyzed using a densitometer to determine the conversion of testosterone to dihydrotestosterone by testosterone 5α-reductase. The results were compared with the control group (conversion rate without adding the herbal extract) to evaluate the testosterone 5α-reductase inhibition rate, and the inhibition rate calculation method was as follows:

Inhibition Rate (%) = [(A-B) / A] × 100

Where

A = conversion of testosterone to dihydrotestosterone (when no herbal extract is added),

B = Conversion of testosterone to dihydrotestosterone (when added herbal extract).

Experimental results by the above inhibition rate calculation method are shown in Table 1 below.

Extract concentration (%) 0.1 0.01 0.001 0.0001 Book 100.0% 100.0% 73.4% 36.8% Schisandra 100.0% 100.0% 71.6% 33.1%

As shown in the results of Table 1 above, the inhibitory effect of the Gobon extract or Schisandra chinensis extract on testosterone 5α-reductase is clear.

Effect Test Example 2 Evaluation of Inhibitory Effect on Hair Growth Stage

Evaluation of the hair growth retardation inhibitory effect test was used 42-56 days old mice (C57BL / 6, male). First, the hair on the back was removed using an electric razor, and each mouse was weighed and divided into 10 pieces so that the weight was evenly distributed. After the adaptation period for one day, from the next day 40% ethanol as a control drug and a concentrated solid of the extract or Schizandra chinensis extract according to the preparation as a test drug were diluted and applied to 40% ethanol. 100 μl was applied to the dorsal site once per day for 35 days.

The result of the test was to determine the ratio of the area in which the newborn hair was grown to the area from which hair was removed using an image analyzer, and then averaged to evaluate the overall hair growth inhibitory effect. Table 2 shows the results of the evaluation of the hair growth inhibitory effect according to the concentration of the extract and Schizandra chinensis extract.

Extract concentration 0.001% 0.01% 0.1% One% 10% Control Herbal extract 28.6 22.8 18.2 14.6 11.2 31.4 Schizandra Extract 29.2 24.6 19.8 15.4 12.4

As shown in Table 2, the extract and Schizandra chinensis extract showed an excellent hair growth transition inhibitory effect in a concentration-dependent manner. That is, at 0.001% or less, the hair growth inhibitory effect was not satisfactory compared to the control group, and at 10% or more, the action was not sufficiently achieved compared to the dose. Therefore, these extracts are preferably used in a concentration of 0.01 to 10% by weight when applied as a hair growth inhibitor.

As shown in the above test results, the extract or Schizandra chinensis extract according to the present invention has a testosterone 5α-reductase activity inhibitory effect, was very excellent in inhibiting the hair growth phase conversion in the animal experimental model.

As described above, the composition of the present invention comprising an extract of Gobon and / or Schizandra chinensis extract having excellent testosterone 5α-reductase activity inhibitory activity and hair growth reversal inhibitory effect as an active ingredient has excellent hair growth inhibitory effect. Therefore, the present invention can be applied not only to products aimed at hair loss and depilation, but also to products aimed at effective management of hair loss after hair loss and depilation.

Claims (2)

A hair growth inhibitory composition comprising at least one of Kobon extract and Schisandra chinensis extract as an active ingredient. The composition of claim 1, wherein the extract and Schizandra chinensis extract are 0.01 to 10% by weight, respectively, as solids, based on the total weight of the composition.