KR20030032983A - Chlorophyll - Google Patents

Abstract

PURPOSE: Provided is a method for extracting chlorophyll from excrement of silkworm at low cost. CONSTITUTION: The method comprises the steps of collecting excrement of five year old silkworm, drying, cleaning, sterilizing and crushing it; preparing alcohol spirits of an alcoholic strength by volume of 95% vol, by fermenting starch of corn or potato; feeding the alcohol of the second step to excrement of the silkworm by 50-70 wt.%; extracting chlorophyll by agitating the solution of the third step in a high speed using an agitator; extracting chlorophyll by feeding dichloromethane to the excrement by 40-70 wt.% and agitating the same in the high speed agitator; performing centrifugation of the chlorophyll to obtain chlorophyll concentrate; emulsifying the chlorophyll concentrate; and commercializing the chlorophyll concentrate emulsion by mixing with water or starch.

Description

Composition for chlorophyll extraction and its preparation method {CHLOROPHYLL}

The present invention relates to a composition for extracting chlorophyll and a method for manufacturing the same, more specifically, extracts chlorophyll from silkworm poultry using an extraction solvent and ultrasonic wave and concentrated in a centrifuge and a vacuum concentrator extract (chlorophyll concentrate), chlorophyll powder and Chlorophyll extraction method and its preparation method for producing a liquid containing chlorophyll.

Conventional chlorophyll is present in the chloroplasts in the cytoplasm of plant cells, absorbing the necessary light for photosynthesis, making carbohydrates through photosynthesis and nourishing the plant to provide the basis of the food chain. It is usually the most common in leaves and sometimes in the green areas of other tissues (stems, roots, etc.).

Conventional extraction method of chlorophyll is used by centrifugation, vacuum concentration extraction, and ultrasonic emulsification using ultrasonic solvent using the extraction solvent ethyl alcohol from the leaves or silkworm dung, the present invention is ultraviolet sterilization, ozone sterilization treatment using silkworm dung After chlorophyll extraction, alcohol 95% is used as the extraction solvent, and extracted by centrifugation and vacuum concentration using only chlorophyll extract solvent methylene chloride (dichloromethane) without using ultrasonic wave, centrifugation, vacuum concentration or ultrasonic wave to emulsify the chlorophyll concentrate. When the ultrasonic wave is usually used, the present invention uses corn oil, which is a fatty acid type, corn oil, polyoxyethylene sorbitol fatty acid ether, and glycerin fatty acid ester. As a method of sterilizing the chlorophyll extract, there is a technical difference in preparing the chlorophyll extract using a colloidal silver solution, which is a silver ion.

(One). Conventional chlorophyll extraction method uses an ethyl alcohol as an extraction solvent to extract the ultrasonic wave. The problem is that the chemical structure of chlorophyll (Fig. 1) and its components are magnesium (Mg) as a bead-like blue-red fine crystal of 5um size To form a chelate form. When UV rays are received in the dark, fluorescence is emitted. Generally, the a and b types are present in higher plants in the range of 3: 1 to 3: 2. Chlorophyll a and b types are present, and tocophenol (vitamin E), nitric acid in fatty acids, Because of the presence of moisture, carbohydrates, vegetable fats, proteins, and carbon, it is difficult to preserve at room temperature and easily decays, so it must be stored frozen. It takes 6-9 hours for a total of 18 hours each, resulting in low productivity, heat generation inside the ultrasonic extractor, which destroys the effective chlorophyll active ingredients, making it difficult to extract chlorophyll completely and increasing manufacturing costs.

(2). In order to solve the above problem (1), the present invention collects silkworm dung silkworm dung silkworm dung collected after drying in a well-ventilated shade of unwinded wind, sprayed with water and washed with UV and ozone (0 ₃ ) Crush.

(3). The pulverized silkworm dung is put into a high-speed rotary stirrer and fermented with corn and sweet potato starch raw materials. The alcoholic content of 95% of the distilled and purified alcohol is added as a silkworm weight ratio by 50-70%. Dichloromethane (CH ₂ Cl ₂₎ , which is a strong chemical formula, was added to the silkworm pore weight ratio by 40-70% as extraction solvent, extracted with stirring in a high speed stirrer, centrifuged in a centrifuge to separate the residue and extract solution. Chlorine extract is extracted by volatilization while stirring in a stirrer, and vacuum concentrated chlorophyll concentrate is extracted in a vacuum concentrator. Characterizing in extracting chlorophyll using an extraction solvent without using an ultrasonic extractor.

(4). As a method of emulsifying the chlorophyll concentrate obtained through the above (2) and (3) process without using an ultrasonic emulsifier, fatty acid (fatty acid) is put into a stirrer of a stainless steel container which is not oxidized to strong acid and strong alkali. Corn oil, polyoxyethlene sorbitol fayyt acid ether, polyoxyethylene glycerine fatty acid ester, etc., using copper, vegetable hardening oil, fatty acid ether, etc. acid ester) to emulsify.

(5) Use tap water to make water with pure reverse osmosis maker that does not contain any mineral (mineral) in water. Prepare water with a pH of 7 or less.

(6). Another feature of the present invention is to use a colloidal silver ion water (colloidal silver ion water) maker using the water of the above (5) to make the silver ion water, the silver sterilization power of various bacteria such as E. coli and virus bacteria This outstanding feature, as already known, has the ability to sterilize over 650 species in at least 6 minutes.

Silver ionized water maker in the present invention is a colloidal silver (colloidal silver) manufacturer using a silver purity of 99.99% of silver decomposed into a colloidal silver 0.005-0.015 micron small particles in 8Occ of pure distilled water dissolved in water, the concentration of 3-5ppm It is in a dissolved state.

(7). The chlorophyll concentrate emulsified in (4) was added to 5-10 gr of colloidal silver ion IL in (6), and the mixture was rapidly stirred in glass containers and plastic containers. The chlorophyll solution in chlorophyll solution was continuously sterilized and kept at room temperature. Suppression of germs does not need to be stored in the refrigerator during distribution.

The sterilization principle of colloidal silver has been confirmed as a natural bactericidal (antibiotic) agent that kills almost all unicellular pathogens on the ground, and silver has a special enzyme that metabolizes (digestion, respiration) of unicellular pathogens. The most influential theory is that disabling and consequently sterilizing the bacteria.

When the chlorophyll liquid prepared above is emulsified by the conventional ultrasonic emulsification, the emulsification time usually takes 6-9 hours to make chlorophyll liquid 6OOL, and the effective component of chlorophyll by heat generated during ultrasonic emulsification is not achieved. This problem is solved in the present invention because it is destroyed and must be stored in the refrigerator during distribution.

(8). As a method of pulverizing the chlorophyll concentrate of (4), starch 6Ogr was added to 10gr of chlorophyll concentrate using potato, corn, and sweet potato starch, sprayed in air at room temperature with high speed stirring in a stainless steel container, and dried with organic and inorganic minerals. Minor chlorophyll powder was added to the mixture.

1 is a chemical structural formula of chlorophyll of the present invention

2 is a chlorophyll extraction process chart of the present invention

3 is a manufacturing process chart of chlorophyll powder of the present invention

4 is a manufacturing process chart of the chlorophyll liquid of the present invention

In the constitution of the present invention, the composition of the present invention is carried out by the same process as the process of manufacturing the chlorophyll extraction process of FIG. 2, the process of manufacturing the chlorophyll powder of FIG. An example will be described in detail as follows.

Make chlorophyll concentrate by extracting 16kg of silkworm dung silkworm dung

As it is,

Example 1

1. Crushed silkworm poop ----- 160kg

2. Alcohol 95% concentration (primary extraction solvent) ----- 50-70% wt

Example 2

1.crushed silkworm ---- 160kg

2. Dichloromethane ---- 40-70% wt

As a composition component to emulsify chlorophyll concentrate

Example 3

1. chlorophyll concentrate ---- 6kg

2. Corn oil-1-3% wt

3. Polyoxyethylene sorbitol fatty acid ether ---- 10-15% wt

4. Polyoxyethlene glycerine fatty acid ester ---- 2-5% wt

As an ingredient to form chlorophyll liquid in emulsified chlorophyll concentrate

Example 4

1.Emulsified Chlorophyll Concentrate --- 6kg

2. colloidal silver ion water --- 6OOL

As a composition ingredient to make chlorophyll from emulsified chlorophyll concentrate

Example 5

1. Emulsified chlorophyll concentrate ---- 10gr

2. starch ---- 60gr

3.Calcium (Ca) --- 3gr

4. Organic Zinc (Zn) --- 1gr

5. Organic Germanium (Ge) --- 0.5gr

In Examples 1 and 2 made of the same process as in Example

In 100gr of the result of component analysis of the produced chlorophyll concentrate

1. Chlorophyll b --- 12.4 mg / 100 gr,

Chlorophyll a --- 30.9mg / 100gr

2. Linoleic acid ---- 22mg / 100gr

3. Tocophenol (Vitamin E) --- 1.6mr / 100gr

4. Carbon powder --- 0.1gr / 100gr

5. Carbohydrates --- 0.3gr / 100gr

6. Lipid --- 0.7gr / 100gr

7. Protein ---- 0.2gr / 100gr

8.Moisture ---- 98.7gr / 100gr

It consisted of, the general viable count was 5.0 * 10 and E. coli was positive.

After making this chlorophyll concentrate from the chlorophyll solution of Example 4, the general bacteria were completely sterilized and E. coli was negative. (Inspection period January 21, 2003)

In the present invention, in the process of extracting chlorophyll using a conventional ultrasonic wave, it is possible to improve the production cost, the destruction of the active ingredient of chlorophyll, the decrease in productivity, etc., in the process of extracting chlorophyll, chlorophyll liquid using water with colloidal silver ion water It can be distributed without the need for refrigeration during manufacturing, and it can be expected to reduce the production cost, improve productivity, and prevent chlorophyll from occurring even when distributed at room temperature.

Claims (4)

1st extraction of composition as extractant for making chlorophyll concentrate from silkworm dung silkworm dung silkworm dung 1.Pulverized silkworm dung --- 160kg, 2. 95% alcohol concentration --- 50-70% wt in stainless steel container After separation by centrifugation, the second extraction and the first extraction leftover residue is put back into the stainless steel container. 1. Add methylene chloride (dichloromethane) --- 40-70% wt. A composition characterized in that the chlorophyll extract concentrate in which the extract was separated from the extract and the primary extract were put together with a stirrer to volatilize the extraction solvent during disturbance, and the remaining chlorophyll extract was concentrated in vacuo with a vacuum concentrator. According to claim 1 As a composition for emulsifying chlorophyll concentrate, 1. Chlorophyll concentrate --- 6 kg, 2. Corn oil --- 1-3% wt, 3. Polyoxyethylene sorbitol fatty acid ether- -10-15% wt, 4. Polyoxyethlene glycerine fatty acid ester-A composition of chlorophyll concentrates characterized by emulsification by the addition of -2-5% wt by rapid stirring. According to claim 2 Composition for making chlorophyll liquid with emulsified chlorophyll concentrate, using pure water with almost no mineral content made with pure distilled water maker. 1.Emulsified Chlorophyll Concentrate --- 6kg, water of 0.005-0.015 micron silver particle made by silver ion water maker. Colloidal silver ion water with 3-5ppm silver content in 800cc. --- 600L, chlorophyll-containing chlorophyll solution made by mixing in a high speed stirrer with a characteristic that is characterized. According to claim 2 As a composition for making chlorophyll powder by adding organic and inorganic minerals to emulsified chlorophyll concentrate, 1.Emulsified chlorophyll concentrate --- 10gr, 2. starch --- 60gr, 3.Calcium (Ca) --- 3gr, 4.Organic zinc (zn) --- 1gr, 5.Organic germanium (Ge) --- 0.5gr Put in a stirrer and stir, spray composition in the air at room temperature, characterized in that the composition of chlorophyll mineral powder.