JP5400476B2 - Spray liquid for pest control agent and method for producing raw material composition of spray liquid - Google Patents

Description

The present invention relates to a natural pest control agent mainly made from neem (common name; NEEM, scientific name Azadirachta Indica A. Juss), a native plant of India that grows naturally in the tropics and subtropics, and a method for producing the same. , A spray solution for pest control agent, which is obtained by enzymatically degrading neem seed contained in at least one main raw material selected from nin (nuclear) and squeezed rice bran and then diluting with water The present invention relates to a method for producing a raw material composition .

  In recent years, there has been a growing interest in environmental destruction and the harmful effects of chemical pesticides have become a problem. As a typical example, a neem pest control agent (hereinafter referred to as a neem agent) mainly composed of an active ingredient azadirachtin (Azadirachtin) contained in neem, which has been attracting attention and used in various countries around the world. Can be mentioned.

  The above neem agent can be obtained by compressing a neem plant at a low temperature and using oil extracted by centrifugation (Patent Document 1), or stirring and mixing an extract containing ethyl alcohol and neem oil and leaving it to stand. In order to use neem oil composition from which the wax fraction is removed (Patent Document 2), there is a disadvantage that the cost must be high. In addition, since azadirachtin itself, which is the main component of neem oil, is easily oxidized, there is also a drawback that the stability during production and storage of the spray liquid is not sufficient.

  Furthermore, since azadirachtin is easily decomposed by sunlight, the effective control period after spraying is short. Therefore, when sprayed frequently in order to obtain a sufficient control effect, there is a disadvantage that not only is it expensive, but also the work load is increased (Patent Document 1). Therefore, its spread is still inadequate, and it has not yet been evaluated as a natural pesticide as a substitute for chemical pesticides.

  In other words, as in the conventional methods, azadirachtin is extracted and purified using an organic solvent such as hexane, ethanol and water, or neem oil is squeezed from neem seeds and seeds. Yield is low. In particular, in the former case, the cost of the extraction liquid such as an organic solvent and the cost for the process are high, so that it has not been able to withstand commercial agricultural use.

US Pat. No. 5,885,600 JP 2008-184453 A

  As a result of intensive studies to make the neem agent resistant to commercial agricultural use, the present inventor uses neem seeds, seeds (nuclear), pomace, etc. as main raw materials, and is contained in the main raw materials. By using an enzyme capable of degrading neem seeds, it is possible not only to efficiently use neem mainly composed of azadirachtin, but also to obtain a composition having good storage stability, an antioxidant, It has been found that by containing an ultraviolet absorber, the effect after spraying as a pest control agent can be sustained for a long time, and the present invention has been achieved.

Accordingly, a first object of the present invention is to provide a spray liquid as an inexpensive neem pest control agent that is excellent not only in storage stability but also in sustained drug efficacy after spraying.
The second object of the present invention is to provide a method for producing a raw material composition for an inexpensive spray solution for neem pesticides, which mainly contains neem seeds, seeds (nuclears), pomace, etc. is there.

That is, the present invention is obtained by enzymatically degrading cereals with at least one main raw material selected from the group consisting of at least neem seeds, seeds (cores), and squeezed strawberries, and strawberries and / or germinating enzymes. The obtained auxiliary material is mixed, the solid in the obtained mixture is pulverized and / or ground and then stirred , and the composition obtained by enzymatic decomposition of the main material is diluted with water. and wherein the fabric-liquid dispersion for pesticides and a method for producing a raw material composition of the diverging coating solution.
In the present invention, from the viewpoint of preserving the preservation effect and the effect after spraying, it is preferable to further contain an antioxidant and / or an ultraviolet absorber, and in particular, an acidic yeast culture solution in which auxiliary materials are dissolved. It is preferable to add.
The acidic yeast culture solution is preferably a yeast culture solution having a pH of 3.0 to 4.0, and particularly preferably the yeast culture solution is a yeast lactic acid bacteria culture solution. Furthermore, the spray solution of the present invention is preferably a filtered spray solution .
Moreover, in the method for producing the raw material composition of the spray liquid for pest control agents of the present invention, the solid pulverization and / or grinding step is performed at a temperature of 30 ° C. or lower in order to reduce the deterioration of the active ingredient. It is preferable.

  The pest control composition or spray liquid of the present invention is produced by using neem, seeds, seeds (nuclear) and pomace as the main raw materials, and enzymatically decomposing the seeds contained in the main raw materials. The extraction residue is reduced and the extraction rate is increased, and the manufacturing method is simple and a large apparatus is not necessarily required. Therefore, the manufacturing cost is reduced. Further, since it can be easily produced and used in the vicinity of the site, it can be used as a natural and safe composition for pest control agents that can replace chemicals.

The figure on the left shows 20 g of neem seed powder, an auxiliary material obtained by enzymatic degradation of cereal with koji enzymes (preferably in an aerobic atmosphere when koji molds are used), and koji enzymes. A total of 30 ml of the acidic yeast culture solution contained is mixed, and is an external photograph substitute figure when it is enzymatically degraded at 50 ° C. for 48 hours. The right figure shows 20 g of neem seed powder in 30 ml of 98% ethanol, It is an external photograph substitute figure when it stirs at 50 degreeC for 48 hours. In the case of enzymatic degradation shown in the left side of FIG. 1, neem seeds are decomposed, neem components are dissolved in the solution, and even though no surfactant is added, small particles (10-10 It is a microphotograph substitution figure which shows a mode that it does not become a large particle | grain by diffusing as it is (30 micrometers).

The spray solution for pest control agent of the present invention is produced by using an active ingredient such as azadirachtin contained in neem without waste, and the form of the raw material composition is powdery, pasty and Any of liquid may be sufficient. As described above, a conventional method of extracting and purifying azadirachtin from neem oil or neem seeds or seeds, or squeezing neem oil from neem seeds or seeds using an organic solvent such as hexane or ethanol and water. Then, in any case, since the yield of the active ingredient is low, the production cost of the neem agent becomes high. In particular, in the former case, the cost of the extract such as an organic solvent and the cost for the process are high.

Hand upon preparing a raw material composition of the spray liquid of the present invention is therefore, inexpensive, neem seed, Hitoshi (nucleus), the pomace or the like as a main raw material, decomposing neem contained in these main raw enzyme To make neem seeds enzymatically decomposed. As a result, the unit price of neem agent, which is a pest control agent, can be greatly reduced.

  As the main raw material used in the present invention, harvested seeds or seeds (nuclear) collected from the seeds may be used as they are, or squeezed rice cakes obtained by squeezing neem oil from these may be used. Use seeds that are easy to handle and preserve. When preserving the seeds, in order to prevent the oxidative degradation of the active ingredients such as azadirachtin, either as a vacuum pack enclosed with an oxygen scavenger or a pack filled with carbon dioxide or nitrogen gas It is preferable to store at 0 to 5 ° C.

  The enzyme for degrading neem seed used in the present invention is not particularly limited as long as it has its action, but it is particularly preferable to use a koji enzyme and / or a germinating enzyme produced by koji mold. The koji mold is cultured using rice, koji, cereal bran, oil and fat, molasses and the like as nutrient sources, and it is particularly preferable to cultivate in an aerobic atmosphere at 25 ° C to 45 ° C. Examples of the culture method include a normal rice bran method in which steamed rice is inoculated with koji mold, a method using koji or cereal bran in the medium, and a liquid culture method.

Hand upon preparing a raw material composition of the spray liquid of the present invention, particularly, the use of black koji (Aspergillus niger) is citric acid producing bacteria, it is possible to prevent contamination by bacteria in the reaction system preferable. Moreover, this Aspergillus niger also has the advantage that ferulic acid esterase that liberates ferulic acid that functions as an antioxidant described later can be produced from cereals (cereal meal). In the present invention, the present invention is not limited to the above Aspergillus niger, and naturally koji molds having the same function and effect can be preferably used.

When water is added to the koji mold cultured by the above method, the koji enzyme is eluted in the solution. In this invention, it is preferable to use a yeast culture solution instead of the said water, and it is preferable that pH of this yeast culture solution shall be 3-4. Moreover, it is preferable to perform this pH adjustment using lactic acid bacteria.
Such an embodiment is a preferable embodiment because contamination by various bacteria is suppressed by ethanol produced by yeast and lactic acid derived from lactic acid bacteria or citric acid derived from Aspergillus.

Hand upon preparing a raw material composition of the spray liquid of the present invention cereals pasty obtained by enzymatic decomposition by koji enzymes and / or germination enzymes, powdered or liquid compositions and auxiliary materials. Preferred examples of the cereals include wheat and beans, corn and the like in addition to rice and rice bran, but the present invention is not limited to these specific examples.

  The paste-like auxiliary material is, for example, added to rice bran (cereal bran) 10 to 15% by mass of water, inoculated with koji mold, then stirred and aerated and fermented with rice bran enzyme, separately prepared yeast lactic acid solution It can be obtained by dissolving rice bran, killing the koji mold, and mixing with a crude enzyme solution obtained by self-digestion. The filtrate obtained by filtering from this paste-like auxiliary material is a liquid auxiliary material.

  The powdery auxiliary material can be obtained, for example, by inoculating a koji mold on heat-sterilized rice koji (cereal koji) and aerating the koji mold while properly adding water and nutrients. As the above-mentioned heat sterilization method, a method of roasting rice bran (cereal rice bran) is a preferable method because a partially pyrolyzed rice bran tissue becomes a nutrient source necessary for the growth of koji mold. In this case, rice bran is decomposed by an enzyme supplied from the dying gonococcus and an antioxidant such as ferulic acid is released. If the powdery auxiliary material obtained in this way is liquefied, it becomes a paste-like auxiliary material.

  The liquid auxiliary material is, for example, inoculated with koji molds in an aqueous solution containing nutrients such as rice bran, molasses, fats and oils, preferably containing nutrients such as phosphorus and potash, and then aerated and agitated, preferably with sterile air. While culturing koji molds, koji molds that are killed one after another are self-digested, and can be obtained by adjusting a solution (or paste-like) in which koji enzymes are diffused in the solution. The resulting solution may be filtered to remove solids.

The spray solution for a pest control agent of the present invention contains at least one main raw material selected from the above-mentioned neem seeds, seeds (core), and squeezed straw, and cereals, preferably in an aerobic atmosphere. In addition, the auxiliary material obtained by enzymatic decomposition with the koji enzyme and / or the germinating enzyme is mixed with an appropriate amount of moisture as necessary, and the solid content in the obtained mixture is preferably obtained using a mill or the like. Is pulverized and / or ground at 30 ° C. or lower, and the paste-like reaction system is further subjected to an enzymatic decomposition reaction at 60 ° C. or lower, preferably 10 to 30 ° C., and then the obtained raw material composition is washed with water. it can be readily obtained by Rukoto be diluted.
When the reaction temperature exceeds 30 ° C., the reaction rate of the enzymatic decomposition can be accelerated, but the deterioration of the active ingredient also proceeds. When the reaction temperature exceeds 60 ° C., the main enzyme is deactivated. This enzymatic decomposition reaction is preferably performed in an anaerobic atmosphere.
Further, depending on the amount of moisture used, the obtained raw material composition can be substantially powdered, or can be in any form such as paste, emulsion, or liquid.

It is preferable that the cereal used as the raw material of the auxiliary material contains germ. In this case, by adding appropriate moisture, various enzymes (germinating enzyme) starting from gibberellin produced in the germ can be produced, and auxiliary materials containing this can be produced. The germinating enzyme not only decomposes Neem Jin but can also efficiently elute useful substances such as γ-oryzanol and fatty acids contained in the cereals, especially the germs thereof, into the component protection liquid. The above-mentioned γ-oryzanol is a ferulic acid ester compound having an antioxidant power, and ferulic acid esterase, which is an enzyme produced by moths such as Aspergillus niger, can release ferulic acid from γ-oryzanol.
However, when water is added to the germ, gibberellin is produced as described above, so that this gibberellin is also contained in the final spray solution. This gibberellin has the merit of increasing the production of vegetables, tea leaves and the like, but it has a demerit when it is desired to suppress the growth of plants such as during seedling raising.

  Therefore, if you want to suppress plant growth, such as when raising seedlings, use raw materials that do not contain germs during the preparation of secondary raw materials, or raw materials that have been heat-treated to inactivate germs. However, it is preferable to prepare auxiliary materials. The same applies to the preparation of “component protection solution” described later.

  In the present invention, from the viewpoint of workability, it is possible to adjust the hardness of the paste by adding water as appropriate, but instead of mere water, the cereals that have undergone enzymatic decomposition by the koji enzyme and / or germination enzyme are dissolved. It is preferable to add an acidic, preferably pH 3.0-4.0 yeast culture solution containing the koji enzyme as a component protection solution that prevents oxidation of the pest control component. The pH can be appropriately adjusted using a known inorganic acid and / or organic acid, but it is particularly preferable to use an organic acid, and it is particularly preferable to use lactic acid.

  In the present invention, it is preferable to add ethanol when generation of carbon dioxide is recognized as a result of grinding the solid content in the mixture obtained by mixing the main raw material and the auxiliary raw material such as Neem Jin. In this case, or in addition to ethanol, known salts, acids, high-concentration sugars and the like can be added to suppress the activity of the yeast that causes carbon dioxide generation.

Thus , the paste-like composition that is the raw material composition of the spray liquid of the present invention can be obtained by pulverizing and / or grinding the solid content in the main raw material and the auxiliary raw material that have absorbed and swelled moisture. Since it can be easily produced by reaction, it is inexpensive. Moreover, since the apparatus which manufactures the said paste-form composition is also simple and cheap, this can also reduce the manufacture unit price of the spray liquid of this invention .

  The above pulverizing and / or grinding means can be appropriately selected from known means such as a stone mill or a mixer. Since the main raw materials such as Neem Jin are softened by absorbing moisture in advance, a simple means such as a stone mortar is sufficient, and there is no particular need for an advanced pulverizing apparatus to make fine particles. However, in order to prevent oxidation of target components such as azadirachtin, it is preferable to add an antioxidant during pulverization, and the process is controlled so that all materials are cooled to 0 ° C to 5 ° C. It is preferable. Furthermore, it is preferable to control so that the temperature of the reaction system after passing through the pulverizer does not exceed 30 ° C.

The above antioxidant can be appropriately selected from known ones, but it is particularly preferable to add an aromatic organic acid having antioxidant ability such as ferulic acid or phytic acid derived from rice bran. These antioxidants are added from the beginning of the mixing of the main component and the subcomponents not only to prevent the active component from being oxidized in the pulverization process but also to prevent the active component from being oxidized in the enzymatic decomposition process of the main component. It is preferable to keep it. By doing so, a highly viscous paste-form composition for pest control agents in which the target substance is finally diffused into water can be obtained. To this paste-like composition, a solution (the component protection solution) obtained by dissolving a paste-like auxiliary material in an acidic yeast culture solution (preferably yeast lactic acid bacteria culture solution) is further diluted with water as necessary. In particular, it is possible to obtain an emulsion composition containing 10 to 30 μm oil particles which is stable without adding a surfactant.
In addition, for example, when the amount of the component protection solution used is sufficient due to the use of the liquid auxiliary material, or when the component protection solution is further added during the preparation of the spray liquid, other antioxidants are further added. There is no need to add.

Spray solution or emulsion composition that is a concentrated solution of the present invention, when the paste composition is already contains the component protective agent, simply by diluting with water it, or the paste-like can be in the composition production process may add water as appropriate, instead of adding or water diluted with water to a single, diluted with the component protective solution, or is preferably obtained by the addition. This is because the neem component can be prevented from being oxidized and deteriorated by sunlight (particularly, ultraviolet rays).

When the paste-like composition or emulsion composition according to the present invention is stored, it is preferably stored sealed in an anaerobic environment. In this case, in order to suppress the generation of carbon dioxide by the yeast in an anaerobic atmosphere and the generation of gas by other bacteria, it is preferable to contain ethanol so as to have a constant concentration. As another method, the paste-like composition may be dehydrated to obtain a substantially powdery composition, which may be stored in a cool and dark place under a nitrogen or carbon dioxide atmosphere or as a vacuum pack.

  In the enzymatic decomposition reaction of Neem Jin according to the present invention, ethanol is added to the paste in order to avoid deterioration of active ingredients due to biological metabolism in the reaction paste (or solution, hereinafter simply referred to as paste). By adjusting the pH to be as low as 3.0 to 4.0, an inanimate enzymatic reaction can be performed, thereby causing the decomposition of neem and specific substances.

  In the present invention, as described above, by adding an aromatic organic acid having antioxidant ability such as ferulic acid derived from sputum enzyme, deterioration of the active ingredient due to oxidation during the enzymatic degradation period can be suppressed. Therefore, if the amount of enzymatic decomposition of rice bran added before enzymatic decomposition is not sufficient, the content of the aromatic organic acid described above is insufficient. In such a case, the antioxidant should be applied before spraying. It is preferable to add to.

  As described above, an aromatic organic acid having antioxidative ability such as ferulic acid can be obtained from rice bran (wheat bran and corn, soybean etc.). In the present invention, moisture and temperature are added to the rice bran, so that the enzyme production of the germ contained in the rice bran starts. Furthermore, the concentration of an aromatic organic acid having antioxidative ability such as ferulic acid can be increased by allowing the soot enzyme to act. However, in order to obtain organic acids such as ferulic acid and phytic acid more efficiently, it is preferable to use a germinating enzyme from the beginning together with the koji enzyme in the present invention. In particular, when using rice bran containing no germ, it is necessary to add a germinating enzyme.

If the paste composition or emulsion composition as the raw material composition of the spray liquid of the present invention thus obtained is diluted , the spray liquid of the present invention is obtained. In the present invention, in order to prevent clogging of the spraying device at the time of spraying, it is preferable to appropriately filter at any stage of the raw material composition such as paste composition, emulsion composition, or sprayed liquid. A filtration device may be added to the device. The spray solution of the present invention contains ferulic acid and / or phytic acid and other organic acids obtained from rice bran (wheat, bran, corn, soybean, etc.), so it is effective in controlling pests with oxygen and sunlight after spraying. Deterioration of components can be suppressed, and the number of spraying per year can be reduced, so that chemical drugs can be fully combated.

  Hereinafter, the present invention will be described in more detail on the basis of examples. However, the present invention is not limited to these examples, and modified inventions using well-known techniques and other differences from the present invention apply. Inventions corresponding to the design matters of the trader are also included in the present invention.

Example In this example, first, 50 kg of rice bran (rice koji, rice bran, bran rice cake) was mixed with 120 L (liter) of an aqueous solution of yeast lactic acid bacteria containing nutrients such as molasses, and the koji was dissolved at 10-15 ° C. The pH was kept at 3.0 to 4.0 to suppress the propagation of various bacteria. At this time, breeding of miscellaneous bacteria is suppressed by ethanol produced by yeast and lactic acid derived from lactic acid bacteria or citric acid derived from Aspergillus. The mixture was pressed to obtain 100 L of a yellow transparent candy enzyme solution.

  Aromatic organic acids typified by ferulic acid and phytic acid are often contained in grains such as rice, wheat and beans. As a method for extracting the aromatic organic acid, there is a method using subcritical water or supercritical gas in addition to enzymatic decomposition or thermal decomposition. In the present invention, it is preferable to use a method of decomposing with a cheap germination enzyme or koji enzyme and a solution method using the functions of ethanol and fatty acids produced by yeast. The solution thus obtained functions as a component protection solution.

  Specifically, an appropriate amount of water or 4 L of yeast solution was added to 20 kg of rice bran containing embryos to promote the activity of the germs, and enzymes were produced. The yeast solution preferably contains koji mold or koji enzyme. The rice bran, which has been pyrolyzed and self-digested, was dissolved in 70 L of yeast solution containing rice bran enzyme, lactic acid bacteria, and molasses to elute the enzyme digestion product of rice bran and to help the growth of yeast and lactic acid bacteria, thereby suppressing the growth of miscellaneous bacteria. The resulting moromi-like liquid was filtered and squeezed, and 50 L of the obtained liquid was used as a component protection solution. This component protection solution contains an antioxidant and an ultraviolet absorber.

  100 L of the sputum enzyme solution and 50 L of the component protection solution were mixed. At this time, if carbon dioxide is generated due to the low ethanol concentration, an appropriate amount of ethanol is added to stop the generation of carbon dioxide. Instead of adding ethanol, known salts, acids, high-concentration sugars, etc. may be added.

  On the other hand, husks were removed from the seeds of neem to obtain the seeds (nuclei). 150 kg of the obtained bean was soaked in 150 L of a mixed solution of the above koji enzyme solution and component protective agent solution for 6 to 24 hours. This is because the stored seeds are hardened in a semi-dried state, so that they absorb and soften water. After supplying water, it is cooled to 0 ° C. to 5 ° C. so that the temperature of the reaction system can be suppressed to 30 ° C. or lower even if the heat generated in the process of crushing and / or grinding the solid content is absorbed. preferable.

  The device for pulverizing and / or grinding the solid content to form a paste may be appropriately selected from known devices such as a stone mill (or a ceramic pulverizer using the principle), a mixer, a roller, and the like. . When using pulling water (a liquid for replenishing water when grinding seeds), it is preferable to use the component protection solution instead of pulling water. Therefore, it is preferable to cool the component protection solution to 0 to 5 ° C. in the same manner as the nin. In this step of making a paste, it is easy to oxidize the surrounding oxygen, so it is preferable to operate the entire device to make a paste in a carbon dioxide or nitrogen gas atmosphere. In addition, since the said component protection solution is reducing, oxidation is suppressed by using this as drawing water.

  Next, the obtained paste was filled in a sealed container and enzymatically decomposed in a dark room at 10 to 30 ° C. In this step, cellulose decomposes cell walls, amylase decomposes starch, and fats and oils decompose lipase. It is thought that the action of fatty acids and lecithin contributes to the fact that the fat and oil component finally diffuses into water. In addition, the active ingredient is also eluted in ethanol contained in the enzyme solution and the component protection solution.

  After it is determined that the target active ingredient has been sufficiently extracted by enzymatic degradation, undegraded seeds and undegraded materials such as stones and foreign matter that are unintentionally mixed are removed as necessary. And stored in a cool dark place or a refrigerator. When the above removal is not performed, when the solution is diluted with water to form a spray solution, it is only necessary to pass a filter having an opening of about 0.5 mm.

  By mixing the paste and the component protective agent before spraying, component deterioration after spraying can be further suppressed. Based on this premise, the amount of component protective agent added before paste processing is reduced to a small amount, saving the amount of ethanol added for adjustment, and shortening the work time. Can be enjoyed. In other words, when the amount of the component protective agent is insufficient, it is necessary to add it before spraying.

  When adjusting the spray liquid by diluting with water, it is preferable to first add the paste-like composition of the present invention to an appropriate amount of the component protective agent and stir well, and then diffuse it into water at an appropriate dilution ratio. In addition, when the component protection liquid is not added (spraying liquid B) and when the component protection liquid is not contained (spraying liquid C), as shown in Table 1 below, when the component protection liquid is added (spraying liquid A) ) Less effective, but in a practical range.

  In the conventional method, neem oil (fat obtained by squeezing bean) is often emulsified and dispersed, but the component protection solution used in the present invention has a strong emulsifying action and emulsion stability, Neem oil and component protection solution can be emulsified by mixing them with a stirrer such as a mixer while keeping the temperature at 25 ° C to 35 ° C.

Comparative Example 10 kg of Neem Jin was ground in 10 L of 98% ethanol. After leaving the obtained paste-like composition for 20 days, 1200 ml of a 20% solution of polyoxyethylene alkylphenyl ether (APE) was added as a surfactant and emulsified by stirring at 25 ° C. Subsequently, it diluted 175 times and filtered and it was set as the spraying liquid D. The concentration expressed per kg of bean was 350 L / kg bean.

  The numerical values in the table are the results of spraying at Sakai Tea Garden. The spraying interval and the amount of sprayed liquid / kg jin (reciprocal of the necessary amount of shin per unit spray volume) can be applied to a higher concentration of azadirachtin, especially in the case of Kanohosoga and Chahamaki, compared to Kanzawa spider mites and Komikan aphids. It means the condition that can control the necessary pests. The ability to suppress here means that the number of inhabitants can be suppressed to a density that does not affect the yield. In addition, it has been demonstrated that the larger the numerical value, the larger the spraying amount per hit, that is, the wider spraying is possible.

According to the method for producing a raw material composition according to the present invention , not only the oxidative deterioration of the active ingredient in the production process of the neem agent can be suppressed, but also the oxidative deterioration of the spray liquid of the present invention after spraying and sunlight (particularly ultraviolet rays). Therefore, the unit price of the medicine is reduced and the number of times of spraying is reduced, so that the work load is reduced. As a neem pesticide with commercial utility that can be converted to a conventional chemical agent, it is not desirable to spray synthetic chemical pesticides such as agricultural use, pest control in residential areas, schools, roadside trees, home gardens, etc. Therefore, the present invention is extremely useful industrially because it is expected to stabilize yield and reduce health damage in poor rural areas.

Claims (14)

At least one main raw material selected from the group consisting of at least seeds, seeds (nuclear) and squeezed potatoes of neem, and a secondary raw material obtained by enzymatically degrading cereals with cocoon enzymes and / or germinating enzymes The mixture is obtained by pulverizing and / or crushing solids in the obtained mixture and stirring, and the composition obtained by enzymatic decomposition of the main raw material is diluted with water , Spray solution for pest control agents. Furthermore, the spray liquid for pest control agents according to claim 1, further comprising an antioxidant and / or an ultraviolet absorber. The main raw material and with auxiliary materials comprising a mixture of yeast culture acidic containing water and / or koji enzymes, according to claim 1 or 2 pesticide spray solution as described in. The spray solution for pest control agents according to claim 3, wherein the acidic yeast culture solution is a yeast culture solution having a pH of 3.0 to 4.0. The spray solution for pest control agents according to claim 4 , wherein the yeast culture solution having a pH of 3.0 to 4.0 is a yeast lactic acid bacteria culture solution . Said distributing liquid, Ru filtered spray liquor der, pesticides spray solutions according to any of claims 1 to 5. At least one main raw material selected from neem seeds, seeds (nuclear), and squeezed rice cakes, and a secondary material obtained by enzymatically degrading cereals using rice bran enzyme and / or germinating enzyme The pesticidal agent according to any one of claims 1 to 6, wherein the main material is enzymatically decomposed after pulverizing and / or grinding the solid in the obtained mixture. A method for producing a raw material composition of a spray liquid. The method for producing a raw material composition for a spray solution for a pest control agent according to claim 7, wherein the solids in the mixture are pulverized and / or ground at a temperature of 30 ° C or lower. The pulverizing agent for pest control according to claim 7 or 8, wherein an acidic yeast culture solution containing pulling water and / or koji enzyme is appropriately added when pulverizing and / or grinding the solid in the mixture. A method for producing a liquid raw material composition. The method for producing a raw material composition for a spray solution for a pest control agent according to any one of claims 7 to 9, wherein the acidic yeast culture solution is a yeast culture solution having a pH of 3.0 to 4.0. . The method for producing a raw material composition of a spray solution for a pest control agent according to claim 10, wherein the yeast culture solution having a pH of 3.0 to 4.0 is a yeast lactic acid bacteria culture solution. The method for producing a raw material composition of a spray solution for a pest control agent according to any one of claims 7 to 11, wherein the auxiliary raw material is prepared in an aerobic atmosphere. The method for producing a raw material composition of a spray solution for a pest control agent according to any one of claims 7 to 12, wherein the enzymatic decomposition of the main raw material is carried out at 10 to 30 ° C. The method of manufacturing the raw material composition of the spray liquid for pest control agents according to any one of claims 7 to 13, wherein the enzymatic decomposition of the main raw material is performed in an anaerobic atmosphere.