KR20010036047A - Cultivation Bottle for Cultivating Mushroom and Method for Cultivating a Mushroom Using the Bottle - Google Patents

Abstract

PURPOSE: A culture bottle for the cultivation of mushroom capable of cultivating mushroom two times or more by pouring a medium in a culture bottle only one time and a method for cultivation of the same are provided, which can reduce cultivation period and control an amount and quality of mushroom. CONSTITUTION: The culture bottle comprises a bottle opened at the upper part and lower part, and a cap for sealing the opened lower part. After mushroom is harvested by using the culture bottle, the bottle is turned over and then cultivation is carried out in the second stage.

Description

Cultivation Bottle for Cultivating Mushroom and Method for Cultivating a Mushroom Using the Bottle}

[Industrial use]

The present invention relates to a culture bottle for mushroom cultivation, and more particularly, to a culture bottle and a mushroom cultivation method using such a culture bottle to allow the culture medium contained in the culture bottle to be used for mushroom cultivation in mushroom culture during mushroom cultivation. It is about.

[Prior art]

Recently, mushroom consumption is increasing every year due to the increase of income and the improvement of dietary culture, and the growing area of artificial mushrooms is increasing day by day.

As a method of artificially cultivating mushrooms, a box cultivation method using a fungus and a bottle cultivation method using a bottle are known.

Among these, the bottle cultivation method, unlike the conventional cultivation method, is capable of producing and cultivating the plant's air conditioning and heating facilities completely without being influenced by the temperature of the outside air throughout the year. It can be processed automatically and can save labor and clean working environment.

Types of mushrooms grown by this cultivation method include Flammulina velutipes 1 and 2, leaf mushrooms (Griofora frodonsa), Auricularia auricula, Lyophyiium ulmarium 1 and 2, and willow. Agrocybe aegerita, Pleurotus ostreatus, Pleurotus eryngii, etc. are known, and many mushrooms can be cultivated by bottle growing.

In general, a mushroom cultivation method using a known culture bottle is first mixed with sawdust and rice bran as a medium material, and then adjusted to moisture, and then put it into the culture bottle and plug it with a stopper. Then, the microorganisms contained in the medium are sterilized in a sterilizer so that only the mushrooms grow well, and the mushrooms to be grown in the inoculation room are inoculated. And incubate the mycelia to the right degree in the culture chamber, and when the spawn culture is completed, open the bottle cap, scrape off the surface of the medium to cut the mycelia to promote the fruiting body generation. As described above, the seedling culture is completed and placed in the cultivator with the lid open to grow the desired mushrooms. After collecting the grown mushrooms, the culture bottle is left in the cultivator to grow the mushrooms up to 2nd or 3rd. .

The general shape of the culture bottle used to artificially cultivate mushrooms by the bottle cultivation method is as shown in FIG.

As shown in Fig. 3, the conventional culture bottle is a plastic bottle made of polypropylene or polyethylene, and is composed of a lid 10 and a bottle 20.

However, when the mushroom is artificially cultivated in the conventional culture bottle as shown in FIG. 3, the nutrients of the medium in the upper side of the culture bottle are depleted by one growth, so the mushrooms can be grown only at the entrance of the culture bottle. Can be grown. Therefore, when cultivating mushrooms in a conventional culture bottle, the medium contained in the culture bottle cannot be used again and the nutrient source in the medium cannot be used efficiently.

In some cases, mushroom cultivation farmers are trying to grow twice with one medium, but unlike the first mushrooms, the second mushrooms are not harvested and the growth period of mushrooms is also doubled.

As such, when the second mushroom grows, the yield decreases and the growth period is the first growth. The nutrient at the entrance of the culture bottle is depleted and the culture bottle is used as it is without the need for replenishment of new nutrients. Because you can not supply enough nutrients.

An object of the present invention is to solve the problems of the prior art as described above, an object of the present invention to improve the structure of the culture bottle to provide a culture bottle capable of growing two or more mushrooms in one medium bottle. .

Still another object of the present invention is to provide a culture bottle which can adjust the quantity and quality of mushrooms to be grown by improving the structure of the culture bottle.

Still another object of the present invention is to provide a mushroom cultivation method which can grow mushrooms two or more times with one medium bottle by using the improved culture bottle as described above.

1 is a perspective view showing a culture bottle according to the present invention,

Figure 2 is a perspective view showing a culture bottle of another embodiment according to the present invention,

Figure 3 is a perspective view showing a conventional culture bottle.

[Means for solving the problem]

In order to achieve the above object, the present invention provides a culture bottle with a lid on both the top and bottom. In order to achieve another object of the present invention, there is provided a culture bottle in which at least one auxiliary culture sphere is formed on the culture bottle shoulder. And in order to achieve another object of the present invention, by growing the culture medium in the above-mentioned culture bottle to grow the mushrooms first, the mushrooms to grow the mushrooms in the state of inverting the culture bottle or inverting only the culture medium in the culture bottle. Provide a cultivation method.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

Figure 1 shows an example of a culture bottle produced according to the present invention.

The biggest feature of the culture bottle according to the present invention is that a culture bottle made of two parts of a bottle and a bottle cap is conventionally made of three parts of an upper lid 10, a bottle 20, and a lower lid 30.

In the present invention, unlike the prior art, by installing the lower lid 30 at the lower portion of the pathogen 20, the culture medium in which the mushroom spawn is sufficiently cultured can be used for mushroom growth.

That is, when mushrooms are grown by using a normal culture bottle, the mushrooms are grown by absorbing only the nutrient source and water in the upper part of the culture bottle. The medium in the bottom remains intact.

Therefore, the present invention is to install the lower lid on the bottom of the embryo bottle so that the culture medium in the lower part of the culture bottle can be reused for mushroom growth, the culture bottle grown in the first and the lower lid is removed or the culture medium in the culture bottle to the lower culture bottle When the mushrooms are grown second by pulling them upside down and reinserting them into the culture bottle, the mushrooms can be grown with almost the same quality and duration as the first grown mushrooms.

In addition, the culture medium after completion of spawn culture usually scrapes off the surface of the medium to remove contaminants and promotes fruiting, but the culture bottle once grown is not contaminated because its bottom surface is blocked from the outside air. By using the culture bottle according to the invention, it is possible to germinate mushroom fruiting bodies immediately without scratching the bacteria.

Hereinafter, a more detailed structure of the culture bottle according to the present invention will be described in the order of the upper lid 10, the bottle 20 and the lower lid 30.

The upper lid 10 has a double structure and a filter is inserted into the double structure to prevent bacteria from penetrating as the inside and outside of the culture bottle are ventilated.

To this end, an outer vent 13 is formed at the lower edge of the upper lid 10 and an inner vent 14 is formed inside the lid, and a sponge 11 is inserted into the double lid inner space as a filter. .

And inside the fastening portion of the upper lid 10 is formed in a curved form in which the engaging jaw 15 is short-circuited is fastened with a bottleneck part of the bottle 20 and one-touch.

The bottle 20 according to the present invention has a bottleneck at the top but is made in an open form at the bottom. And the bottle shoulder 23 part is formed inclined at 5-15 degrees. In this way, the upper and lower pathogens are formed in an open form so that the mushroom can be grown two or more times.

Here, the bottleneck 10 is formed at the outer edge of the upper bottleneck inlet of the bottle 20 so that the bottle cap 10 can be closed by a one-touch type.

In addition, the shoulder portion of the top of the pathogen 20 forms a plurality of auxiliary cultures 25 of the protruding form, preferably 3 to 4 are suitable. The auxiliary culture port 25 is attached to the auxiliary stopper 27 to block the entry and exit of the outside air, and the auxiliary culture port 25 forms a jaw to prevent water infiltration. In this case, the jaw height of the auxiliary culture port 25 is appropriately 2 to 4 mm in the case of a 1000 cc culture bottle.

As such, forming the auxiliary culture section 25 in the pathogen is for controlling the yield and quality of the mushroom. That is, when the mushroom is grown while the upper lid 10 is sealed and the auxiliary culture opening 25 is opened, the grown mushroom can grow a small number of mushrooms but a long and coarse quality mushroom, and the upper lid. If the mushrooms are grown in a state in which both the (10) and the auxiliary culture ports 25 are left open, a plurality of mushrooms can be grown in a short time.

And the lower portion of the bottle 20 is formed with a screw thread 28 therein to fasten the lower lid (30). Here, the thread 28 is formed in a form in which each thread 28 is shorted in order to use the lower lid 30 as a one-touch type and a 90 ° rotating screw type.

The lower lid 30 according to the present invention is the bottleneck portion of the bottle 20 and the upper lid form that is integrally combined, that is, when the lower lid 30 is coupled to the bottle 20 forms a symmetrical upper and lower culture bottles Is produced by. And the outer diameter of the coupling portion of the lower lid 30 is the same as the inner diameter of the bottle 20 is produced to be combined into the bottle. Here, the outer portion of the coupling portion of the lower lid 30 is formed with a screw thread 31 for coupling with the bottle, the lower portion is formed with a handle 35 so as to open and close the lower lid 30. The handle also serves as a stand so that the bottom is flat so that the culture bottle does not fall. In addition, a protrusion 32 is formed at the outer lower end of the lower lid coupling portion, and the sealing rubber ring 33 is fixed thereon. The lower lid 30 is formed to be coupled to the inside of the bottle 20 in order to effectively prevent the moisture in the cultivator from flowing into the outside of the bottle 20 when penetrating into the culture bottle.

2, the modification of the culture bottle by this invention is shown.

As shown in Figure 2 modified example of the present invention is the upper lid 10 and the upper portion of the bottle 20 is the same as described above and the structure of the lower lid 30 is fastened to the lower portion of the bottle 20 is There is a variant.

Compared with FIG. 1, the modified example shown in FIG. 2 is manufactured so that the lower part of the bottle 20 is formed in a symmetrical shape in which a bottleneck is formed like the upper part of the bottle, and the lower lid 20 is manufactured to be coupled to the outside of the bottle 20. The point is different.

In addition, there is another difference in that the sealing rubber ring 33 is double installed on the bottom surface of the bottle 20 and the bottom surface of the lower lid to enhance the airtight effect.

The following shows an example in which cultivated mushrooms of oyster mushroom using the culture bottle according to the present invention to help the understanding of the present invention.

The secondary cultivation method of mushrooms using a culture bottle according to the present invention is divided into a culture medium flip method that inverts only the medium and a culture bottle flip method that inverts the culture bottle itself, and the primary culture method of the mushrooms is the same.

In the first mushroom cultivation, the oyster mushroom was cultivated using the culture bottles shown in FIGS. 1 and 2, and the culture bottle of FIG. 1 was used in the medium flipping method during the second cultivation, and FIG. 1 in the culture bottle flipping method. Using both the culture bottles of Figure 2 and the second large cultivated mushrooms.

The following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

Example

The mushrooms cultivated using the culture bottle according to the present invention are Pleurotus eryngii belonging to the genus Pleurotus eryngii.

First, the mushroom cultivation medium was used by mixing sawdust, bran and corn tilting 10: 3: 0.5. Here, sawdust is selected from cedar, gingko, red oak, buckwheat, oak, oyster oak, west tree, maple, Japanese lotus, redwood, rhododendron, camphor and coniferous sawdust for 3 months. For months, we used wild vegetables and hardwood sawdust was used. The higher the water content of the medium was, the longer the mycelia elongated, the more bacteria were attached.

The blended medium was placed in the culture bottles of Figures 1 and 2 produced by the present invention. Here, the culture bottle was a culture bottle of 1000cc capacity made of polypropylene (PP).

The granulation process of the medium is somewhat different depending on the secondary cultivation method of the mushroom.

First, in the case of inverting the medium, the lower lid was fastened to the pathogen in the culture bottle shown in FIG. 1, and the auxiliary culture port was sealed with the auxiliary stopper. Then, about 600 g of mixed medium was granulated and the culture bottle was sealed with the upper lid.

In the case of the culture bottle flipping method, the culture bottle shown in FIG. 1 is inserted with a vinyl with a thickness of 0.01 to 0.05 mm between the lower lid and the bottle, and the lower lid is closed, and the culture bottle shown in FIG. In the state of fastening the lid immediately, the mixed medium 600g was granulated and the culture bottle was sealed with the upper lid.

All the sealed culture bottles were transferred to a sterilization chamber and autoclaved at 121 ° C. (pressure 1.4 kg / cm 2) for about 1 hour to sterilize various germs contained in the medium.

After sterilization, the medium was cooled and inoculated with about 15 cc of each large incubator seedlings purchased from a sterilized clean room using a spoon or inoculator.

After the seeding inoculation in the medium, the mycelia were incubated for 25-30 days. The temperature in the culture chamber was maintained at 20-22 ° C., the humidity was 65-70%, and the concentration of carbon dioxide was adjusted to 2,000-3,000 ppm.

When the incubation was completed, the culture bottle was transferred to the cultivator and arranged on the fungus with the upper lid open.

And the bacteria were scraped to remove the aged inoculum inoculated on the surface of the medium to align the germination of the fruiting body. Care should be taken when scratching the bacteria, so that the water in the medium evaporates during the culturing process, so that the surface of the medium is dry, supplying sufficient moisture.

Scratch-free media develops fruiting bodies after 7 to 10 days in the grower. The Greater Oyster Mushroom is highly sensitive to ventilation, so the air in the grower was ventilated for 10 minutes every 2 hours. The temperature was maintained at 16-18 ° C., the humidity was maintained at 85-90%, and the light was continuously supplied.

After two to three days after the fruiting, the fruit was grown to a mushroom with a normal shape of 1 to 2 mm in length, and further harvested for about 5 to 7 days. The primary yield was 100-150 g per culture bottle.

After harvesting the cultivated large elder mushroom first, the top surface of the medium was usually scraped without inverting the culture bottle, and the second growth was performed under the same conditions as the first growing condition. As a result, it took 20 days to complete the secondary growth and the yield was 50-60g per culture bottle.

However, according to the medium or culture bottle flip method according to the present invention, the yield of the second-larger mushroom was grown more than the second crop without culturing the culture bottle.

Secondary cultivation of mushrooms in this example was as follows.

First, in the case of inverting the medium, when the culture bottle shown in FIG. 1 of the first harvest is taken and the lower lid is opened, the culture medium is contained on the lower lid. Then flip the culture medium in the lower lid and put it back into the bottle. At this time, the culture medium is symmetrical in shape, and is relatively firmly solidified so that the medium is not disturbed. In this way, only the culture medium is changed while the culture bottle is in the same state. In this state, the lower part of the bottle was sealed again with the lower lid, and the second growth was performed under the condition that the mushroom growth conditions were the same as the primary growing conditions. As a result, it took 10 days for the second growth to be completed and the yield was 100-120 g, similar to the first yield.

Next, in the case of inverting the culture bottle by the culture bottle of FIG. 1, the first harvested culture bottle is held, the upper lid is closed, and the external air is completely blocked, and then the culture bottle is inverted and the lower lid is opened. Will be in a state. To prevent the vinyl from peeling off, tie it with a string at the bottom of the bottle, and then cut the vinyl into several crosses so that the large oyster mushroom can grow. In this state, the mushroom growth conditions were secondary growth under the same conditions as the primary cultivation conditions. As a result, it took 11 days to complete the second growth and the yield was 90-110g.

Finally, in the case of inverting the culture bottle by the culture bottle of FIG. 2, the first harvested culture bottle is held, the upper lid is closed, and the outside air is completely blocked, and the culture bottle is turned over, the lower lid is opened, and the mushroom growth condition is 1 Secondary growth was carried out under the same conditions as the tea cultivation conditions. As a result, it took 10 days to complete the secondary growth and the yield was 100-130 g, similar to the primary yield.

In the present invention, the structure of the culture bottle is formed in the upper and lower symmetry, and the cap is installed at the lower part so that the mushroom can be grown two or more times by one influx of medium, and the quality and yield of the second grown mushroom are first grown. It can keep the mushroom growing time while keeping the same.

In addition, it is possible to control the quantity and quality of the mushrooms to be grown by forming a secondary culture in the culture bottle.

In this way, by using the culture bottle according to the present invention, after the first harvest of the mushroom, by providing a mushroom cultivation method of inverting the culture bottle and growing second, the medium can be used efficiently and economically.

Claims (12)

In the bottle mushroom cultivation bottle consisting of a bottle cap and a bottle, A bottle mushroom cultivation bottle, characterized in that the upper and lower portions are formed of a bottle which is open, and a lower lid that seals the bottom of the open bottle. According to claim 1, wherein the bottle of the culture bottle is a bottleneck is formed in the upper portion and the lower portion of the bottle mushroom culture culture bottle, characterized in that open in a form extending in a straight line with the body. According to claim 2, wherein the shape of the lower lid bottleneck shape bottle cultivation culture bottle, characterized in that formed in a symmetrical shape combined with the bottle cap and the bottle. According to claim 3, wherein the lower lid bottle bottle cultivation culture bottle, characterized in that the lower portion of the bottleneck shape is formed as a handle and the pedestal. According to claim 1, wherein the pathogen of the culture bottle is a bottleneck culture bottle for cultivating the mushroom, characterized in that the bottleneck is formed in the upper and lower portions of the bottle and the symmetrical shape of the bottle. The method of claim 5, wherein the lower lid is formed in the same shape as the bottleneck formed on the lower portion of the bottle bottle culture culture bottle for mushroom cultivation, characterized in that the bottom portion of the bottleneck is formed integrally with the handle cap. The method of any one of claims 2, 3, 5, and 6, wherein a short-circuit-shaped thread is formed at the lower lid coupling portion and the lower part of the bottle body to rotate the lower lid to 90 degrees or less to open and seal the lower portion of the bottle. Bottle mushroom culture culture bottle, characterized in that it can be. The method of claim 7, wherein the bottle cap culture culture bottle characterized in that at least one sealing rubber ring is fixed to the inner or outer side of the lower lid coupling portion. The auxiliary stopper according to any one of claims 2, 3, 5, and 6, wherein an auxiliary stopper is formed at a shoulder portion connecting the bottleneck and the body of the culture bottle path to seal the at least one auxiliary culture and the auxiliary culture. A culture bottle for growing bottle mushrooms. 10. The method of claim 9, wherein the auxiliary culture is a bottle mushroom culture culture, characterized in that formed by protruding from the bottle shoulder surface. According to any one of claims 2, 3, 5, 6, wherein the bottle cap inner coupling portion and the upper bottle neck outer coupling portion is formed in the form of a closed curve can be opened and closed the bottle cap in the vertical direction A culture bottle for growing mushrooms. Entering a culture medium into a culture bottle, sterilizing the culture medium and inoculating mushroom seedlings, incubating the inoculated culture medium with mycelium in the culture chamber and placing the culture bottle in which the mycelium is cultured in the growth chamber to grow mushrooms In the cultivation method, After harvesting the first mushroom, the mushroom growing method, characterized in that the second growth of the mushroom by placing the lower surface of the culture medium in the culture bottle to the top.