JPH1084772A - Cultivation method for ganoderma lucidum (fr.) karst. - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cultivation method for Ganoderma lucidum (Fr.) Karst. capable of efficiently obtaining the Ganoderma lucidum (Fr.) Karst. by protecting a nursery bed against the infiltration of general bacteria. SOLUTION: Media mainly composed of sawdust are housed in a vessel body and are sterilized. Seed bacteria are inoculated and cultured in these media. When the primordium of a fruit body B arises on the surface of the media, the media A contg. the primordium of the fruit body B are taken out of the vessel body and are transplanted into a pot 1 for cultivation. Inorg. sand (KIRYU sand 2) subjected to a sterilization treatment is then packed into this pot for cultivation so as to cover the surface of the primordium of the fruit body B. Struts 3 are therefore, erected at the pot 1 for cultivation and a transparent plastic bag is put from above as a cover member 4, by which the Ganoderma lucidum (Fr.) Karst. is cultivated.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating ginseng mushrooms which are useful as auspicious mushrooms or herbal medicine.

[0002]

2. Description of the Related Art Artificial cultivation of Ganoderma lucidum, called Reishi, which is prized as a Chinese herbal nourishment and tonic in combination with other crude drugs, uses cultivation of raw wood by sandalwood and a medium consisting of sawdust. Sawdust cultivation is known.

[0003] In cultivating sawdust of Mannentake, a wide-mouth medicinal bottle or a wide-mouth container or bag made of polypropylene is filled with a medium mainly composed of sawdust, which is then sterilized, inoculated with a seed fungus, and maintained at a temperature of 20 to 30 ° C. When the culture is maintained and maintained, the mycelium spreads over the entire medium in about 20 days. By continuing the cultivation, a hyphal mass serving as a fruit body primordium is formed on the surface of the medium in about 10 to 20 days. When the fruit body primordium is formed and grows, a plug having air permeability, such as a cotton lump that has closed the opening of the container, is removed, or a gap is formed between the plug and the mouth of the container. The umbrella is formed and the umbrella is differentiated and grows to grow into a mushroom.

[0004]

The cultivation of sawdust of Ganoderma lucidum is relatively easy in about one year from the inoculation of the inoculum to the growth and collection in the same manner as in the method of cultivating a bed of shiitake and oyster mushrooms. Although it can be cultivated, there is a problem that it is sensitive to invasion of various germs during the growth of the fruiting body, and the growth is inhibited by various germs and pests, so that it is not possible to obtain the desired quality of Ganoderma lucidum. Water depletion also affects growth. In particular, in the case of fungal bed cultivation, it is common to use a medium by storing a medium in a container body composed of a wide-mouth bottle and sterilizing, inoculating a seed fungus, filling a cotton lump in the mouth of the wide-mouth bottle, and using the filter as a filter. However, various bacteria floating in the air enter the wide-mouthed bottle and inhibit the growth of hyphae.In the extension of the handle after the formation of the fruit body primordium, the cotton plug closing the mouth of the container must be removed. It is necessary to remove or form a gap between the mouth and the cotton plug, so that germs in the air can contaminate the culture medium and handle and inhibit the growth of the fruiting body primordium. There was a problem.

SUMMARY OF THE INVENTION [0005] In view of the above-mentioned circumstances, an object of the present invention is to protect a bacterial bed from invasion of various bacteria when cultivating Ganoderma velutipes, and to efficiently cultivate the fungus bed. Still another object of the present invention is to provide a method for cultivating Ganoderma velutipes which can maintain excellent temperature and humidity at a predetermined temperature and obtain excellent quality of Ganoderma lucidum under cultivation in an appropriate environment.

[0006]

Means for Solving the Problems In order to achieve the above object, a method for cultivating Ganoderma velutipes according to the present invention is characterized in that a container mainly contains a medium mainly composed of sawdust, sterilized, inoculated with a seed fungus, and cultured. When the fruit body primordium is generated on the surface of the seedling body, the medium containing the fruit body primordium is taken out from the container body and stored in the cultivation pot, and inorganic sand is covered in the cultivation pot to cover the surface of the fruit body primordium. And then cultivated by a conventional method.

[0007] In still another cultivation method of Ganoderma velutipes of the present invention, a medium containing mainly sawdust is contained in a container body, sterilized, inoculated with a seed bacterium and cultured, and the fruit body primordium is generated on the surface of the medium. The medium containing the fruiting body primordium is taken out of the container body and housed in a cultivation pot, and the cultivation pot is filled with inorganic sand so as to cover the surface of the fruiting body primordium, It is characterized in that the cultivation is performed by covering the upper part with a transparent or translucent cover member.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The method for cultivating Ganoderma velutipes according to the present invention is based on conventional fungal bed cultivation, and is characterized in that the inoculum inoculated in the culture medium grows without being contaminated by various bacteria. From the viewpoint of preventing invasion of various germs, the container body used for the cultivation of the bacterial bed is preferably of a wide-mouth type having a mouth portion smaller in diameter than the body portion, and the material may be glass, but it is economical. From the aspect, a plastic bag such as polypropylene, or a plastic bag may be used.

[0009] The medium to be used is broadleaf tree sawdust such as oak, oak, oak, mizunara, abemaki, cherry, plum, and pseudopox, and rice bran is added thereto at a weight ratio of sawdust: rice bran of about 10: 1.5. A predetermined amount is put in a plastic container body, sterilized by heating under normal pressure or high pressure, and then inoculated with a seed of Ganoderma lucidum. At this time, a gas-permeable filter made of cotton lump or the like that closes the container body is also sterilized at the same time.

The medium inoculated with the inoculum is cultivated for about 3 weeks in a room at a temperature of 20 to 30 ° C. so as to prevent entry of various bacteria in the air. At this time, if various germs enter the container body, the germs vigorously proliferate and inhibit the growth of hyphae of Ganoderma velutipes. Therefore, sufficient management is required. By maintaining the temperature at about 20 ° C. even after the mycelium spreads throughout the entire medium, the fruit body primordium consisting of the bumpy hypha mass is formed on the surface of the medium in about 10 to 20 days.

When the fruit body primordium consisting of the mycelial mass is formed on the surface of the medium, the medium is taken out of the container body and transferred to a flowerpot-shaped cultivation pot having an open top. This cultivation pot is filled with inorganic sand that has been sterilized in advance, and the extracted fruiting body primordium is filled therein, and the gap formed between the cultivation pot and the fruiting body primordium is filled with inorganic sand. At the same time, the surface of the fruit body primordium is covered with sand. The transfer of the culture medium to the cultivation pot is desirably performed in a sterile room free of various bacteria. However, in practice, it is preferably performed early in the morning or at midnight when there are few bacteria floating in the air.

The main reason for covering the surface of the fruit body primordium with sand is to prevent various bacteria from adhering to the fruit body primordium when the fruit body primordium comes into contact with air. Therefore, the sand covering the fruiting body primordia may be at least as long as it can prevent the fruiting body primordium from being directly exposed to air, and its thickness may be at least about 1 to 2 mm, and it is not necessary to cover much soil. Absent.

[0013] The inorganic sand used for the covering soil is mainly river sand which does not contain corrosive organic substances, especially river sand upstream of a river in which sand has a corner and an appropriate gap can be formed by the corner. preferable. Kiryu sand, Fuji sand, Tenjin river sand used for gardening
Mincho sand and the like can be mentioned. In addition, sea sand can be used besides such river sand, but when using sea sand, it is necessary to wash away salt. In addition, these inorganic sands that have been sterilized in advance are used.

Cultivation pots in which the fruit body primordium is covered with inorganic sand are arranged on a shelf or the like and further cultivated while maintaining a temperature of 24-28 ° C. and a humidity of 70-90%, whereby the fruit body primordium grows, From the fruit body primordium, a column-shaped handle with a yellowish-white tip extends, from which the umbrella differentiates and develops growing Ganoderma lucidum. At that time, by covering the opening of the cultivation pot with a transparent or translucent plastic cover member, drying of the culture medium and the fruit body primordium can be prevented, and the temperature can be kept constant.

The shape of the cover member is not particularly limited as long as it is large enough to cover the growing fruit body primordium. For example, three or four cultivation pots may be used. A support may be built so that its height is about 20 cm, and a plastic bag may be attached to the support.

[0016]

According to the method for cultivating Ganoderma lucidum of the present invention, a medium having a fruit body primordium formed on the surface is transplanted into a cultivation pot,
By filling the cultivation pot with sand covering the fruit body primordium, the fruit body primordium and the culture medium can be prevented from being contaminated with various bacteria. In addition, since the temperature and humidity can be controlled by covering the upper part of the cultivation pot with a transparent or translucent cover member, it is possible to more easily cultivate Ganoderma radishes without any germs.

[0017]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the method for cultivating Ganoderma velutipes according to the present invention will be specifically described below with reference to the accompanying drawings. Fresh rice bran is added to the sawdust of kunugi in a weight ratio of about 10 to 1.5.
, And water was added to form a medium having a moisture content of about 65%. About 1 kg of this medium was packed in a cylindrical polypropylene culture bag, and was about 12 cm in diameter and about 15 cm in height.
And compacted so that After the medium was sterilized by heating under normal pressure or high pressure together with the culture bag, the medium was inoculated with a seed of Ganoderma lucidum. After the inoculation, the medium in the culture bag is placed in a house at a temperature of 20 to 30 ° C and a humidity of 60.
The mycelium was cultured in an environment of about 70% (RH) while appropriately irradiating light, and the mycelium was spread throughout the medium in about 20 days. The culture bag in which the hypha was spread throughout the medium was subsequently placed in a relatively bright room at a temperature of 20 ° C. or more, whereby a mushroom fruit body primordium covered with a hyphal membrane was formed.
At this point, the culture medium A was taken out of the culture bag, and the upper part was opened, as shown in FIG.
On the bottom of the cultivation pot 1, Kiryu sand 2 is spread with a thickness of about 2.5 cm, and a cylindrical medium A is placed at the center of the cultivation pot 1 on the Kiryu sand 2, After filling Kiryu sand 2 into the gap formed between the inner peripheral wall of the cultivation pot 1 and the outer periphery of the culture medium A to keep the culture medium A stationary, the culture medium A
Soak Kiryu sand 2 to a thickness of about 2 mm so that the surface of the fruit body primordium is completely hidden on the surface of the medium.
And covered with soil. Then, four pillars 3, 3,... Are erected on the Kiryu sand near the upper inner periphery of the cultivation pot 1, and a plastic transparent bag is covered as a cover member 4 on the pillow, and the cultivation pot 2 is placed thereon. A space having a height of about 20 cm was formed.
In this state, the cultivation pot 1 is heated at a temperature of 24 to 28 ° C. and a humidity of 7 ° C.
It was controlled so as to be 5 to 85% to achieve the occurrence of the fruiting body and the growth of the fruiting body. During that time, since the cover member 4 was transparent, the growth condition of the fruiting body could be observed and managed every day. By the cultivation management for about 6 months, the columnar tip of the mycelial mass and the yellow-white handle elongated, and the umbrella differentiated from the tip of the handle to obtain the Ginseng mushroom B that grew smoothly.

[0018]

The method for cultivating Ganoderma lucidum according to the present invention comprises
At the time when the fruit body primordium was formed on the surface of the culture medium, the culture medium of Ganoderma lucidus obtained by inoculation and culture of the inoculum by a conventional method was removed from the culture vessel and transferred to a cultivation pot containing inorganic sand, and the fruit body primordium was removed. The soil is covered with sand in the cultivation pot so that the surface of the seedlings is covered, and then the fruiting body primordium is grown by a conventional method. The cultivation can be performed in a state where the temperature is lowered.

Further, by covering the upper part of the cultivation pot in which the surface of the fruit body primordium is covered with sand with a transparent or translucent cover member, cultivation can be carried out while keeping the temperature and humidity more constant. In addition, it is possible to more easily and easily cultivate garland mushrooms with various germs.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a cultivation example of a cultivation method of Ganoderma lucidum according to the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Cultivation pot 2 Kiryu sand 3 Prop 4 Cover member A Medium B Fruit body

Claims (3)

[Claims] 1. A container body containing a medium mainly composed of sawdust, sterilized, inoculated with a seed bacterium and cultured, and when a fruit body primordium is generated on the surface of the medium, the medium containing the fruit body primordium is placed in a container. Taking out of the main body, storing it in a cultivation pot, filling the cultivation pot with inorganic sand so as to cover the surface of the fruit body primordium, and then cultivating it by a conventional method . 2. A container body containing a medium mainly composed of sawdust, sterilized, inoculated with a seed bacterium and cultured, and when a fruit body primordium is generated on the surface of the medium, the medium containing the fruit body primordium is containerized. Removed from the main body, housed in a cultivation pot, and filled the cultivation pot with inorganic sand so as to cover the surface of the fruit body primordium, and the upper part of the cultivation pot with a transparent or translucent cover member A cultivation method of Ganoderma velutipes, characterized by being covered and cultivated. 3. The method according to claim 1, wherein the inorganic sand is river sand that has been sterilized.