KR20100041668A - Method of cultivation of lentinus edodes - Google Patents

Method of cultivation of lentinus edodes Download PDF

Info

Publication number
KR20100041668A
KR20100041668A KR1020090074825A KR20090074825A KR20100041668A KR 20100041668 A KR20100041668 A KR 20100041668A KR 1020090074825 A KR1020090074825 A KR 1020090074825A KR 20090074825 A KR20090074825 A KR 20090074825A KR 20100041668 A KR20100041668 A KR 20100041668A
Authority
KR
South Korea
Prior art keywords
box
cultivation
weight
shiitake
culture
Prior art date
Application number
KR1020090074825A
Other languages
Korean (ko)
Inventor
문상영
차준돈
고동일
차주훈
차주희
Original Assignee
문상영
차준돈
차주훈
차주희
고동일
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 문상영, 차준돈, 차주훈, 차주희, 고동일 filed Critical 문상영
Publication of KR20100041668A publication Critical patent/KR20100041668A/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/62Racks; Trays
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/64Cultivation containers; Lids therefor
    • A01G18/66Cultivation bags
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/64Cultivation containers; Lids therefor
    • A01G18/68Cultivation bottles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/69Arrangements for managing the environment, e.g. sprinklers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Mushroom Cultivation (AREA)

Abstract

PURPOSE: A method for cultivation of shiitake in an air permeable cultivation box is provided to cultivate the shiitake for 35 ~ 40 days by inoculating shiitake on a culture medium consisting of sawdust, cotton seed hull, corn cob, and rice bran. CONSTITUTION: A method for cultivation of shiitake in an air permeable cultivation box(2) comprises the following steps: filling a culture medium in a selected cultivating container; sterilizing filling materials with high temperature steam of 110~120°C; cooling the filling materials to 15~20°C; and cultivating shiitake spawn in a culturing chamber for 15 ~ 20 days by inoculating the shiitake spawn on the cooled culture medium. The culture medium comprises sawdust 70 weight%, cotton seed husk 10 weight%, corn cob 10 weight%, and rice bran 10 weight%.

Description

표고버섯 상자 재배방법{Method of cultivation of lentinus edodes}Method of cultivating shiitake mushroom box {Method of cultivation of lentinus edodes}

본 발명은 표고버섯을 일정온도와 습도를 맞춘 재배실의 상자에서 재배하는 표고버섯 상자 재배방법에 관한 것인데 보다 구체적인 것은 톱밥, 면실피, 콩코브, 미강으로 조성된 배지를 만들고 배양용기에 충전하여 살균한 후 버섯종균을 접종하여 배양실에서 15~20일간 배양한 다음 배양용기에서 분리하고 배양물 전부를 통기성 재배상자에 담아 일정온도와 습도하에 발아시키고 발아후에 계속적으로 육성시키는 표고버섯 상자(균상) 재배방법이다.The present invention relates to a method of cultivating shiitake mushroom box for cultivating shiitake mushrooms in a box of a cultivation room adjusted to a certain temperature and humidity, and more specifically, by making a medium made of sawdust, cotton bark, soybean cob, rice bran, and filling the culture vessel. After sterilization, inoculate mushroom seedlings, incubate in culture room for 15-20 days, separate from culture container, put all cultures in breathable growing box, germinate under constant temperature and humidity, and cultivate continuously after germination (normal) Cultivation method.

버섯은 무공해 식품으로 성인병에 좋고 항암효과가 있는 건강식품임이 입증되면서 날로 그 수요가 증가되고 있으며 특히 표고버섯은 우리나라에서 식용으로 널리 재배되고 있는 현실에 있다.As mushrooms are pollution-free foods and proved to be healthy foods that are good for adult diseases and have anti-cancer effects, the demand is increasing day by day, and shiitake mushrooms are widely cultivated for food in Korea.

그러나 종래의 표고버섯 재배 방법은 참나무 원목에 구멍을 뚫고 버섯종균을 접종하여 재배하는 원목재배법과 톱밥에 영양제를 첨가한 톱밥배지를 재배용기(내열성 비닐봉지 또는 플라스틱 용기)에 충전한 다음 버섯종균을 접종하여 육성시키는 톱밥재배법이 이용되고 있다.However, the conventional shiitake mushroom cultivation method is to cultivate the oak wood and inoculate mushroom seedlings, and to cultivate the sawdust medium added with nutrients to sawdust in a cultivation container (heat-resistant plastic bag or plastic container) and then mushroom seedlings Sawdust cultivation is inoculated and grown.

그러나 이러한 재배방법은 일손이 많이 드는 어려움이 있고 재배공간을 많이 차지하게 되는 등의 불편이 있으며 재배기간이 오래 걸리는 불편이 있다.However, such a cultivation method is difficult to take a lot of work, such as taking up a lot of cultivation space, there is an inconvenience that takes a long growing period.

본 발명은 종래의 결점을 개선하여 35~40일만에 수확이 가능한 능률적인 재배방법을 개발한 것이다.The present invention has been developed an efficient cultivation method that can be harvested in 35-40 days by improving the conventional drawbacks.

본 발명은 배양용기 내에 톱밥, 면실피, 콩코브, 미강으로 혼합구성된 배지를 충전시키고 살균하여 표고버섯종균을 접종시켜서 배양시킨 다음 바늘구멍이 천공된 고밀도 폴리에틸렌필름을 주벽과 바닥에 배치시킨 통기성 재배상자에 옮겨서 발아시키고 그 상태로 육성시켜서 표고버섯을 재배하는 방법이다The present invention is filled with a culture medium consisting of sawdust, cotton thread, soybean cob, rice bran in a culture vessel and sterilized by inoculating shiitake mushroom spawn, followed by cultivation of air permeable high-density polyethylene film on the circumferential wall and bottom. It is a method of growing shiitake mushrooms by transferring them to a box, germinating them, and growing them in that state.

이와 같이 된 본 발명은 원목재배법이나 용기재배법에 비하여 일손을 크게 줄일 수 있고 생산면적의 활용성을 높일 수 있고 재배기간을 단축시켜서 35~40일 만에 수확이 가능케 되는 이점이 있다.The present invention as described above has the advantage that can be greatly reduced compared to the wood cultivation method or container cultivation method, can increase the usability of the production area, and can be harvested in 35 to 40 days by reducing the cultivation period.

본 발명은 톱밥 70중량%, 면실피 중량%, 콩코브 10중량%, 미강 10중량%로 혼합 구성한 배지를 내열성 비닐봉지 또는 플라스틱용기 중에서 선택한 배양용기에 충전하고 110~120℃의 고압증기로 살균하여 15~25℃냉각시킨 후 표고버섯종균을 접종하여 (1병당 800~900g접종)배양실에서 15~20일간 배양하는 종균 접종 배양단계와 The present invention is filled with a medium consisting of sawdust 70% by weight, cotton silk weight, soybean cob 10% by weight, rice bran 10% by weight in a culture vessel selected from a heat-resistant plastic bag or plastic container and sterilized by high pressure steam of 110 ~ 120 ℃ After cooling 15 ~ 25 ℃, inoculated with shiitake mushroom seedlings (800 ~ 900g inoculation per bottle) and seeding inoculation step of culturing for 15 ~ 20 days in culture room.

바늘구멍(3a)이 천공된 고밀도 폴리에틸렌필름(3)를 주벽과 바닥에 부착시키고 크기가 가로세로 40cm되고 깊이 10~20cm 되며 주벽과 바닥에 구멍이 형성된 통기성 재배상자(2)를 제조하는 재배상자 준비단계와 A growing box for attaching a high density polyethylene film (3) having a needle hole (3a) attached to the main wall and the floor, and having a size of 40 cm in width, 10 to 20 cm in depth, and a permeable growing box (2) having holes in the main wall and the bottom. Preparation and

상기 통기성 재배상자(2)를 온도 18∼22℃로 유지되고 습도 75~90%를 유지하는 재배실의 선반위에 배치하고 각 상자속에 표고버섯 종균접종 배양물(4)을 옮겨서 상자상면을 통기성 폴리에틸렌필름으로 덮어 발아시키는 버섯발아 단계와 The breathable cultivation box (2) is placed on a shelf of a cultivation room maintained at a temperature of 18 to 22 ℃ and maintains a humidity of 75 to 90%, and the shiitake mushroom seed inoculation culture (4) is transferred to each box to provide a breathable polyethylene Mushroom germination step covered with film

상자에서 버섯이 발아되어 배지가 갈변하면 상자 상면을 개방하고 버섯이 자라도록 하는 육성단계로 이루어지는 표고버섯 상자 재배방법이다.When mushrooms germinate in a box and the medium is browned, it is a method of cultivating a shiitake box, which is a growing step of opening the top of the box and allowing the mushroom to grow.

(실시례)(Example)

본 발명은 가로세로 길이가 40cm되고 깊이가 10~20cm 되는 통기성 재배상자(2) 주벽과 바늘구멍(3a)이 천공된 고밀도 폴리에틸렌필름(3)를 부착한 재배상자를 온도 18~22℃, 습도 75~90% 되는 재배실의 선반 위에 배치하여 각 상자 내부에 표고버섯 종균 배양물을 옮겨서 상자 상면을 통기성 비닐지로 덮고 발아시켜서 버섯이 보이고 배지가 갈변이 되면 상자 상면에 덮은 통기성 비닐지를 벗기고 계속 육성시켜 표고버섯을 재배한다.The present invention is a culture box attached to a high-density polyethylene film (3) perforated the periphery wall and the needle hole (3a) of the permeable growth box 2 40cm in length and 10-20cm in depth, humidity 18 ~ 22 ℃, humidity Place the shiitake mushroom spawn culture inside each box by placing it on the shelf of 75-90% cultivation room, cover the top of the box with breathable plastic paper and germinate so that the mushrooms are visible, and if the medium turns brown, remove the breathable plastic paper covered on the top of the box and continue to grow. Shiitake mushrooms are grown.

이때, 재배실(1)은 도 2와 같이 골조(100) 외면에 비닐지(101)를 씌우고 비닐지 외부에 간격(102)를 두고 하나의 비닐지(104)를 씌워서 지하수 분사장치(103)를 통해 수막이 형성될 수 있게 만들고 외측 비닐지(104)에 카시미론솜이 내장된 이불(105)을 덮고 일부 외부에 차광막(106)을 덮어 내부 온도가 20℃를 유지하고 습도 80%를 유지하도록 하여 버섯을 재배함으로써 표고버섯 종균을 접종하여 배양한 후 45일만에 수확을 할 수 있게 되어 참나 재배보다 15일을 앞당겨 수확이 가능한 효과를 얻었다.At this time, the cultivation room 1 covers the plastic sheet 101 on the outer surface of the frame 100 as shown in FIG. 2 and covers one plastic sheet 104 at intervals 102 on the outer surface of the plastic sheet, thereby allowing the groundwater spraying device 103. To allow the water film to be formed, and to cover the quilt 105 with the built-in plastic sheet on the outer plastic paper 104, and to cover the light shielding film 106 on the outside to keep the internal temperature at 20 ° C. and the humidity at 80%. By cultivating the mushrooms, the seedlings were inoculated with spawn mushroom seedlings, which were harvested within 45 days of incubation, and the harvesting effect was obtained 15 days earlier than the cultivation.

도 1은 본 발명의 톱밥 재배상자 예시도1 is an exemplary view of the sawdust growth box of the present invention

도 2는 본 발명의 재배실 예시도이다.Figure 2 is an illustration of the cultivation room of the present invention.

* 도면의 주요부분에 대한 부호설명* Explanation of symbols on the main parts of the drawings

2 : 통기성 재배상자 3 : 폴리에틸렌필름2: breathable cultivation box 3: polyethylene film

3a : 바늘구멍 4 : 버섯종균 접종 배양물3a: needle hole 4: mushroom spawn inoculation culture

Claims (1)

톱밥 70중량%, 면실필 10중량%, 콩코브 10중량%, 미강 10중량%로 혼합 구성한 배지를 내열성 비닐봉지 또는 플라스틱 용기중에서 선택한 배양용기에 충전하고 110~120℃의 고압증기를 가해 살균한 다음 15~25℃로 냉각시켜서 표고버섯종균을 접종하여 (1병당 800~900g접종)배양실에서 15~20일간 배양하는 종균 접종 배양단계와 A medium consisting of 70% by weight of sawdust, 10% by weight of cotton thread, 10% by weight of bean cob, and 10% by weight of rice bran was filled into a culture vessel selected from a heat-resistant plastic bag or a plastic container and sterilized by applying a high-pressure steam at 110 to 120 ° C. Next, inoculate shiitake mushroom seedlings by cooling to 15 ~ 25 ℃ (800 ~ 900g inoculation per bottle) and inoculate the seeding incubation step of incubating for 15 ~ 20 days in the culture room. 바늘구멍(3a)이 천공된 고밀도 폴리에틸렌필름(3)을 주벽과 바닥에 부착시키고 크기가 가로세로 40cm되고 깊이 10~20cm 되며 주벽과 바닥에 구멍이 형성된 통기성 재배상자(2)를 제조하는 재배상자 준비단계와 Cultivation box for attaching a high density polyethylene film (3) perforated with a needle hole (3a) to the main wall and the bottom, 40cm in width, 10 to 20cm in depth, and a breathable growth box (2) formed with holes in the main wall and the bottom Preparation and 상기 통기성 재배상자(2)를 내부온도가 18∼22℃로 유지되고 습도 75~90%를 유지하게 만든 재배실의 선반에 배치하고 각 재배상자에 버섯종균접종 배양물(4)을 옮겨서 재배상자 상면에 통기성 폴리에틸렌필름을 덮어 발아시키는 버섯발아 단계와 The breathable cultivation box (2) is placed on the shelf of the cultivation room to maintain the internal temperature of 18 ~ 22 ℃ and maintain the humidity 75 ~ 90% and transfer the mushroom seed culture culture (4) to each cultivation box Mushroom germination step to germinate by covering the breathable polyethylene film on the top 상자에서 버섯이 발아되어 배지가 갈변하면 상자 상면을 개방하고 버섯이 자라도록 하는 육성단계로 이루어지는 표고버섯 상자 재배방법Cultivation method of shiitake mushroom box consisting of growing stages in which the mushrooms germinate in the box and the medium is browned to open the top of the box and allow the mushrooms to grow.
KR1020090074825A 2008-10-14 2009-08-13 Method of cultivation of lentinus edodes KR20100041668A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20080100845 2008-10-14
KR1020080100845 2008-10-14

Publications (1)

Publication Number Publication Date
KR20100041668A true KR20100041668A (en) 2010-04-22

Family

ID=42217356

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020090074825A KR20100041668A (en) 2008-10-14 2009-08-13 Method of cultivation of lentinus edodes

Country Status (1)

Country Link
KR (1) KR20100041668A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103168617A (en) * 2013-01-14 2013-06-26 杭州益禾农业科技有限公司 Cut-log cultivation method of polyporusrhinocerus cooke
CN103416225A (en) * 2013-08-08 2013-12-04 刘万顺 Method for preparing high-quality ganoderma lucidum compost
CN107371795A (en) * 2017-07-28 2017-11-24 浙江省农业科学院 A kind of mushroom management of producing mushroom method waterborne

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103168617A (en) * 2013-01-14 2013-06-26 杭州益禾农业科技有限公司 Cut-log cultivation method of polyporusrhinocerus cooke
CN103416225A (en) * 2013-08-08 2013-12-04 刘万顺 Method for preparing high-quality ganoderma lucidum compost
CN107371795A (en) * 2017-07-28 2017-11-24 浙江省农业科学院 A kind of mushroom management of producing mushroom method waterborne

Similar Documents

Publication Publication Date Title
CN105210671B (en) A kind of log glossy ganoderma breeding method
CN103891524B (en) The method of glossy ganoderma dish garden formula cultivation and the medium for cultivating ganoderma
CN102786333A (en) Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same
CN106613355A (en) Ecological planting method of oyster mushrooms
CN109168982A (en) A kind of seafood mushroom liquid strain production of hybrid seeds culture medium, culture medium for cultivating and cultural method
CN105684733B (en) Bag plants needle mushroom breeding method
CN104381024A (en) Planting method of flower mushrooms
CN103881931A (en) Pholiota nameko and culture method thereof
KR101006732B1 (en) Method of cultivation of lentinus edodes
KR20100071155A (en) Method for cultivation of fragrant mushroom
CN105706732A (en) Method for cultivating ganoderma applanatum
CN103907477A (en) Method and medium for cultivating mushrooms in potted landscape manner
CN203942847U (en) The device of potted plant pet mushroom
KR101174245B1 (en) Lentinus edode cultivated by using fermentation medium and cultvation method thereof
CN1322801C (en) Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom
KR20070024791A (en) Method of cultivation gastrodia elata blume
CN110946037A (en) Pleurotus eryngii factory cultivation and breeding method
CN107629966A (en) Fungal elicitor, its preparation method and the method that bletilla striata quick reproduction technique is carried out using the fungal elicitor
CN104303840A (en) Cultivating method for tray-loaded flammulina velutipes
KR20100041668A (en) Method of cultivation of lentinus edodes
KR101028954B1 (en) Mushroom's growing method and mushroom growing box for home
KR100832352B1 (en) Novel stropharia rugosoannulata and methods for cultivation thereof
CN102612989A (en) Method for box cultivation of cordyceps
CN109006169A (en) A kind of production method of batch production side's packet cultivation Dictyophora rubrovalvata
JP5156243B2 (en) A method for cultivating ornamental white to yellow deer antler.

Legal Events

Date Code Title Description
A201 Request for examination
A302 Request for accelerated examination
E902 Notification of reason for refusal
AMND Amendment
E902 Notification of reason for refusal
E601 Decision to refuse application
AMND Amendment