KR20010018659A - A cosmetic composition containing Curcumae Radix extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Curcumae Radix extract is prepared for use as sunblock, which is excellent in scavenging free radicals, absorbing ultraviolet ray without allergy. CONSTITUTION: In the cosmetic composition, Curcumae Radix extract is extracted from Curcumae longa linne, and 0.001-10 wt.% of Curcumae Radix extract is incorporated into cosmetic composition. Curcumae Radix is extracted by using one solvent selected from the group consisting of water, methanol, ethanol, acetone, ethyl acetate, 1,3-buthylene glycol, hexane, diethyl ether, normal propanol. iso-propanol, and normal butanol.

Description

A cosmetic composition containing Curcumae Radix extracts}

The present invention relates to a cosmetic composition containing an extract of Curcumae Radix extracted from a tuber of Curcuma longa linne, which is a Zingiberaceae plant.

In the cosmetic field, oxidative stress caused by free radicals is the most important problem to overcome in order to suppress skin aging. Damage caused by free radicals is widely recognized in the human body and is recognized as the main mechanism of aging.

Free radicals are elements or molecules with one or more unpaired electrons and have the property of stabilizing electrons from other molecules. The lifetime of free radicals is very short (a few thousandths of a second), unstable and very aggressive. Radicals that are involved in aging of the skin include hydroxyl radical, superoxide radical, superoxide radical, singlet oxygen, hydrogen peroxide (H ₂ O ₂ ), and the like. These radicals have strong oxidative power and can react with all substances in the living body. Lipids are attacked to produce lipid peroxides to modify the function of cell membranes, to attack proteins to denature or lose their function, and to attack DNA, a genetic information material. Continued exposure to oxidative stress caused by these radicals can lead to skin aging, sunburn and inflammation caused by sunlight, contact dermatitis, and wrinkles.

The human body is equipped with a defense system to prevent damage caused by free radicals. Vitamin E, vitamin C, Superoxide dismutase (SOD), catalase (Catalase) and various kinds of oxidase (Oxidase) and the like to eliminate free radicals. However, modern people are exposed to the harmful environment of air pollution, unhealthy diet, UV exposure, stress, and diseases, making it difficult to sufficiently eliminate oxidative stress caused by various radicals. Therefore, the substance capable of eliminating free radicals may prevent or delay aging of the skin by removing oxidative stress on the skin.

For the above reasons, the development of a safer and high free radical scavenging effect has emerged as an important task in the field of cosmetics, and many studies have been conducted.

Accordingly, the present inventors conducted a search study on herbal medicines growing in Korea in order to develop a free radical scavenging substance from plants, and found that the free radical scavenging effect of turmeric extract was very excellent, thereby completing the present invention.

Therefore, an object of the present invention is to provide a cosmetic composition containing turmeric extract.

The present invention relates to a cosmetic composition containing Curcumae Radix extract.

The extract of Curcumae Radix, which is characteristically contained in the cosmetic composition according to the present invention, is extracted from dried tubers of Curcuma longa Linne. Zingiberaceae plants include Curcuma wenyujin YH Chen et C. Ling, Curcuma longa Linne, Curcuma kwangsiensis SG Lee et CF Liang, or Curcuma phaeocaulis Val. . Turmeric is a perennial grass that grows in the tropics. The leaves are elliptical, four to five gathered. The rhizome is solid or branched round columnar, and the cut side is dark blue. It is cultivated in all parts of the country except the northern mountainous regions of Korea. The flower is yellowish white cylindrical shape, which blooms from August to November. Wash the turmeric tube in water and dry it in the sun or dry it.

Curcumae Radix extract contained in the composition according to the present invention is prepared from powdered turmeric water, methanol, aqueous methanol solution, ethanol aqueous ethanol solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butyl Len glycol aqueous solution, hexane, diethyl ether, normal propanol, iso-propanol or normal butanol can be extracted using an extraction solvent and concentrated under reduced pressure.

The turmeric extract may be prepared by immersion for 1 to 15 days at 15 ° C. to 40 ° C. under the solvent or after extraction for 1 to 7 days in an extractor equipped with a cooling capacitor, followed by concentration under reduced pressure. In addition, the extraction process may include a low-temperature aging process for 7 to 10 days at 4 to 15 ℃.

In the preparation method of the turmeric extract, the extraction solvent is preferably used in an amount of 1 to 15 times the dry weight of turmeric tuber root.

The turmeric extract prepared as described above may be prepared as a cosmetic composition by a conventional method by adding a conventional additive, and preferably contained in an amount of 0.001 to 10 weights, more preferably 1 to 7 weights, based on the amount of the cosmetics dried. .

The cosmetic composition according to the present invention may be prepared in the form of a basic cosmetic such as flexible lotion, milk lotion, nourishing cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil.

In addition, the cosmetic composition according to the present invention can be prepared in the form of color cosmetics, such as foundation, lipstick, mascara or makeup base.

In addition, the cosmetic composition according to the present invention can be prepared in the form of a hair cosmetic such as shampoo, conditioner, hair conditioner or hair gel.

Hereinafter, the present invention will be described in more detail with reference to Examples, Experimental Examples, Comparative Examples, and Prescription Examples. However, the present invention is not limited thereto.

Example 1

After washing with purified water and drying, 1 kg of powdered turmeric powder was poured into 5 L of water, extracted at about 20 ° C. for 5 days, filtered through a 300 mesh filter cloth, left at 10 ° C. for 10 days, and aged at low temperature. Was filtered. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 51.9 g (dry weight).

Example 2

After washing with purified water and drying, 1kg of powdered turmeric powder was placed in 5 liters of 25 ethanol, extracted with an extractor equipped with a cooling condenser for 3 days, filtered through a 300 mesh filter cloth, left at 10 ° C for 10 days, and aged at low temperature. Filter by filter paper. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 63.2 g (dry weight).

Examples 3-15

To extract the dry weight by the method of Example 2 using a solvent described in Table 1 below as described in Table 1.

division menstruum Dry weight (unit: g) Example 3 50 ethanol 79.9 Example 4 75 Ethanol 86.6 Example 5 100 Ethanol 26.1 Example 6 80 Methanol 95.1 Example 7 100 Methanol 23.8 Example 8 Acetone 16.2 Example 9 Ethyl acetate 16.7 Example 10 501,3-butylene glycol aqueous solution 86.3 Example 11 Hexane 4.3 Example 12 Diethyl ether 1.6 Example 13 Normal-propanol 8.8 Example 14 Iso-propanol 7.9 Example 15 Normal-propanol 7.2

Experimental Example 1

Free Radical Scavenging Effect of Turmeric Extract

1) Experiment Method

Free radical scavenging effect was obtained by dimethyl sulfoxide of turmeric extract in 2 ml of 60 μM 1,1-diphenyl-2-picryl hydrazyl (DPPH) ethanol solution. 2 ml of DMSO) solution was added and reacted at room temperature for 30 minutes, and then the absorbance was measured at 520 nm to determine the free radical scavenging effect (). The free radical scavenging effect of each example extract was calculated by the following formula.

Free radical scavenging effect () = 100-[(absorbance after extract treatment / control absorbance) × 100]

2) Experiment result

Free radical scavenging effect of turmeric extract is shown in Table 2. Turmeric extracts showed high activity.

extract Free radical scavenging effect () Experimental concentration (㎍ / ㎖) 5 25 50 Example 1 15.7 26.4 30.1 Example 2 23.2 29.8 32.4 Example 3 35.2 38.6 38.7 Example 4 46.2 76.7 89.6 Example 5 35.3 75.4 90.1 Example 6 48.1 78.6 84.7 Example 7 50.2 75.3 85.1 Example 8 59.2 81.4 90.3 Example 9 54.3 78.4 84.6 Example 10 45.7 78.8 84.0 Example 11 19.8 32.7 64.7 Example 12 28.4 35.1 45.8 Example 13 31.8 56.5 65.3 Example 14 28.1 46.0 75.4 Example 15 21.4 43.8 63.6

* The above value is the average of 3 runs

Comparative Example 1

Comparison of Turmeric Extract with Existing Free Radical Scavenging Effect

Turmeric extract was prepared from Example 6. Free radical scavenging effect between turmeric extract and conventional scavengers was measured in the same manner as in Experimental Example 1, and the results of comparing SC ₅₀ values are shown in Table 3. SC ₅₀ is the concentration of sample required to remove 50 free radicals.

Test substance SC ₅₀ (μg / ml) Turmeric extract (Example 6) 12.51 Ascorbic acid 29.74 Dibutyl hydroxyl toluene (BHT) 38.05 DL-α-Tocopherol 34.67 Beta-carotene 54.01 Morin 23.61

Comparing the free radical scavenging effect of turmeric extract with conventional scavenging agents, the turmeric extract showed very high activity.

Experimental Example 2

Inhibitory Effect of Turmeric Extract on Hydrogen Peroxide (H ₂ O ₂ ) -induced Cytotoxicity

Inhibitory effect of turmeric extract on hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity was measured using mouse-derived skin fibroblasts. Hydrogen peroxide generates hydroxyl radicals in the cell, causing fatal damage to the cell. The free radical scavenging effect of turmeric extract was measured as follows to test at the cellular level.

1) Experiment Method

Turmeric extract was prepared from Example 6. Mouse-derived fibroblasts (fibroblast, NIH / 3T3) were inoculated into each well (5 × 10 ⁴ ) in 96-well plates and incubated in 200 μl of Dulbecco's Modified Eagle Medium (DMEM) medium for 24 hours. 10 μl of H ₂ O ₂ diluted to a final concentration of 1 mM in the medium was added, and at the same time, the final concentration of the 1,3-butylene glycol (50v / v, aqueous solution) solution of turmeric extract was 0, 10, 50, 100, After 200 μg / ml and incubation for 24 hours, 10 μl of MTT solution (tetrazolium salt 3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) per well was added and left for 4 hours. Discarded, part of the badge. 100 μl of dimethyl sulfoxide (DMSO) solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.

2) Experiment result

Table 4 shows the inhibitory effect on the hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity of turmeric extract. The cytotoxic effect was calculated by the following formula.

Cytotoxic inhibitory effect () = [(absorbance at extract treatment-absorbance at H ₂ O ₂ treatment) / absorbance at H ₂ O ₂ treatment] × 100

Concentration of extract (µg / ml) Cytotoxic effect () 0 0 10 2.09 50 10.37 100 23.64 200 32.90

* The above value is the average of 3 runs

As shown in the results, turmeric extract showed an inhibitory effect on hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity. Therefore, turmeric extract was shown to suppress cytotoxicity by scavenging hydroxyl radicals generated in fibroblasts.

Experimental Example 3

Cytotoxicity of Turmeric Extract The cytotoxicity of turmeric extract itself was measured using mouse-derived skin fibroblasts (AB).

1) Experiment Method

The turmeric extract used in Example 6 was used.

5 × 10 ⁴ mouse-derived fibroblasts were inoculated into 96 well plates and incubated in 200 μl of DMEM medium, so that the final concentration of turmeric extract solution was 0, 50, 100, 200, 500 μg / ml. Added and incubated for 24 hours. After 10 μl of MTT solution was added and left for 4 hours, 100 μl of dimethyl sulfoxide (DMSO) was added to the wells, dissolved for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.

2) Result of experiment

The cytotoxicity of turmeric extract itself is shown in Table 5. Cytotoxicity was calculated according to the following formula as cell viability.

Cell viability () = (absorbance at extract treatment / absorbance at control treatment) × 100

Concentration of extract (µg / ml) Cell viability () 0 100 50 100.24 100 97.65 200 85.61 500 60.81

* The above value is the average of 3 runs

Prescription Example 1

Prescription example of the flexible lotion of the cosmetic containing turmeric extract is as follows.

ingredient content Turmeric extract 5.0 1,3-butylene glycol 6.0 Sodium hyaluronate 2.0 glycerin 4.0 Fiji 4000 1.0 Poly Solvent 20 0.5 ethanol 10.0 antiseptic Quantity Benzophenone-9 0.05 incense Quantity Purified water Quantity Sum 100

Prescription Example 2

A prescription example of milk lotion among cosmetics containing turmeric extract is as follows.

ingredient Content (weight) Turmeric extract 5.0 Stearic acid 0.4 1,3-butylene glycol 6.0 Cetostearyl alcohol 1.2 glycerin 4.0 Glyceryl Stearate 1.0 Triethanol amine 0.25 Tocopheryl acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Poly Solvent 60 1.5 Solbitan Sesquioleate 0.6 Carboxyvinyl Polymer 0.15 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 3

Prescription example of nutritional cream in cosmetics containing turmeric extract is as follows.

ingredient Content (weight) Turmeric extract 5.0 vaseline 7.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 2.0 Stearic acid 1.5 Floating paraffin 10.0 Beeswax 2.0 Poly Solvent 60 1.5 Solbitan Sesquioleate 0.8 Squalane 3.0 1,3-butylene glycol 6.0 glycerin 4.0 Triethanol amine 0.5 Cotoperyl acetate 0.1 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 4

Prescription example of the essence of the cosmetic containing turmeric extract is as follows.

ingredient Content (weight) Turmeric extract 5.0 glycerin 10.0 Fiji 1500 2.0 Allantoin 0.1 Panthenol 0.3 EDTA 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octyldodeceth-25 0.6 ethanol 6.0 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

Prescription Example 5

A prescription example of a massage cream in cosmetics containing turmeric extract is as follows.

ingredient Content (weight) Turmeric extract 3.0 Glyceryl Stearate 2.0 Cetostearyl alcohol 2.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 Squalene 5.0 Mineral oil 35.0 Dimethicone 1.0 Xanthan Gum 0.1 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanol amine 0.5 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

Prescription Example 6

An example of the pack prescription among cosmetics containing turmeric extract is as follows.

ingredient Content (weight) Turmeric extract 3.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 Fiji 1500 2.0 Panthenol 0.4 Allantoin 0.1 ethanol 6.0 Fiji 40 Cured Castor Oil 0.3 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

As can be seen from the above, turmeric extract according to the present invention can not only have a very good free radical scavenging effect, but also has little cytotoxicity and thus can be effectively used in cosmetics.

Claims (7)

Cosmetic composition containing Curcumae Radix extract. The cosmetic composition according to claim 1, wherein the turmeric extract is contained in an amount of 0.001 to 10% by weight based on the dry weight of the cosmetic. The method of claim 1, wherein the turmeric extract is water, methanol and methanol aqueous solution, ethanol and ethanol aqueous solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butylene glycol aqueous solution, hexane, diethyl ether, normal Cosmetic composition, characterized in that extracted with an extraction solvent selected from the group consisting of propanol, iso-propanol and normal butanol. The cosmetic composition according to claim 1, which is a basic cosmetic, color cosmetic or hair cosmetic. 5. The cosmetic composition according to claim 4, wherein the basic cosmetic is softener, milk crocion, nutrition cream, massage cream, essence, cleansing foam, cleansing water, pack, or body oil. The cosmetic composition according to claim 4, wherein the color cosmetic is a foundation, lipstick, mascara or makeup base. The cosmetic composition according to claim 4, wherein the cosmetic for hair is a shampoo, a rinse, a hair conditioner or a hair gel.