KR100570117B1 - Cosmetics for free radical scavenging containing gold extract - Google Patents

Abstract

The present invention relates to a cosmetic having a free radical scavenging action of skin cells containing extracts purified from the root of Cibotium barometz .

The gold moth extract of the present invention is obtained by drying and pulverizing the hawthorn, which is made into fine powder, and then concentrated by depressurizing the extracted solution by immersion at room temperature for 1 to 15 days by adding an extraction solvent.

The cosmetic of the present invention may be prepared in the form of softening (skin), astringent makeup, nourishing cosmetics (lotion), nutrition cream, massage cream, essence or pack.

Free radical scavenging cosmetics of the present invention by eliminating the free radicals of the skin, by inhibiting the oxidation of the biological material by the free radicals to prevent or delay the aging of the skin and is safe because it is a natural substance.

Description

Cosmetics for free radical scavenging containing gold extract

The present invention relates to a free radical scavenging cosmetic containing an extract purified from the root of Cibotium barometz , specifically, free radical scavenging action to prevent or delay skin aging and natural free material It relates to a cosmetic for radical scavenging.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of skin that occurs naturally with age. To this end, research is underway to develop substances that have excellent safety and strong skin beauty effects by searching for the skin physiological activity of various natural plant components.

Aging of the skin is due to oxidative damage of biomaterials by free radicals. In other words, highly reactive free radicals such as hydrogen peroxide, hydroxy radicals, superoxide radicals, and other active oxygen radically oxidize the lipids that make up cell membranes, destroy cell membranes, oxidize proteins, and attack nucleic acids, causing oxidative damage. Destroy and eventually kill the cells.

Thus, preventing or delaying aging of the skin is made possible by eliminating free radicals in the skin.

Ascorbic acid, DL-alpha-tocopherol, beta-carotene, and dibutylhydroxytoluene (BHT) are known to have free radical scavenging effects. have.

Since these substances are mainly used as synthetics, they may cause skin safety problems for long-term use or in large amounts. Therefore, there is an increasing demand for free radical scavenging cosmetics that are safer for skin application.

The present inventors studied various plant extracts in order to search for substances that prevent skin aging. As a result, the extracts obtained from the hair roots of citrus bark , especially Cibotii Rhizoma , were found to have a large free radical scavenging effect. To complete.

Geummo-gucheok is a perennial herb belonging to the Dicksoniaceae, which grows in China and other places, and is known as flavonoids and tannins such as kaempferol. Kwon, pp212, Yegang Publishers, Seoul, 1994)

It is an object of the present invention to provide a cosmetic for free radical scavenging of natural substances having a free radical scavenging action to prevent or delay skin aging.

The present invention relates to cosmetics for free radical scavenging containing extracts obtained from the roots of the hair follicles, especially the hair follicles.

The extract of chamomile extract contained in the cosmetic of the present invention is extracted by a conventional extraction method from the root diameter of chamomile chuck or chamomile chuck.

That is, the root diameter of the hair follicles is dried and finely powdered, and then the extractant is added and then deposited at room temperature for 1 to 15 days to extract the active ingredient. At this time, the extraction solvent may be water, lower alcohol, mixed solvent of water and lower alcohol, acetone, diethyl ether, chloroform, hexane or ethyl acetate, 1 ~ 15ℓ extraction solvent for 1 kg of fine powder of the umbilical root diameter Preference is given to using.

The solution thus extracted is concentrated under reduced pressure to form a gold moth extract, and this is added to the cosmetic.

At this time, the amount of the extract of gold hair chuck is added to the cosmetic is 0.001 to 10% by weight, preferably 0.01 to 5% by weight relative to the cosmetic.

The free radical scavenging cosmetic of the present invention may be prepared in the form of a flexible cosmetic (skin), astringent cosmetic, nutrition nutrient (lotion), nutrition cream, massage cream, essence, pack and the like.

Hereinafter, the present invention will be described in detail, but the present invention is not limited thereto.

Example 1

After washing with purified water and drying, 1kg of the pulverized fine gold pulverized fine powder was put in 3L of water and immersed at room temperature for 15 days, and then filtered with a filter of Whatman 2 times. The filtrate was concentrated in a vacuum condenser with a cooling capacitor to obtain a dry extract of 42.7 g of weight.

Examples 2-14

Except for using the extraction solvent of Table 1, the extraction was carried out in the same manner as in Example 1 to obtain a gold hair extract.

TABLE 1

Experimental Results of Examples 2-14

Experimental Example 1

The free radical scavenging action of the hair extracts prepared in Examples 1 to 14 was measured as follows.

That is, 60 μ M 1,1- diphenyl-2-picryl-2 Hydra quality (1,1-diphenyl-2-picryl hydrazyl, DPPH) dimethyl sulfoxide (DMSO) in the nine geummo extract the ethanol solution 2㎖ After adding 2 ml of the solution and reacting at room temperature for 30 minutes, the absorbance was measured at 520 nm.

The free radical scavenging action was calculated by the following formula.

The results are shown in Table 2 below.

TABLE 2

Free Radical Scavenging Activity of Extracts

* 3 times average

Most of the extracts showed high activity, especially in extracts of solvents with lower polarity than polar solvents such as water or water-containing solvents.

Experimental Example 2 Comparison of Free Radical Scavenging Activity with Extract of Gold Moth Extract and Existing Antioxidants

Examples of the gold moth extract of Examples 1-14 and the existing antioxidants ascorbic acid, ascorbic acid, DL-alpha-tocopherol, dl- α-tocopherol, beta-carotene and dibutylhydroxytoluene (BHT) The free radical scavenging effect was measured in the same manner as in Experimental Example 1 to compare IC ₅₀ , a concentration exhibiting a 50% inhibitory effect.

The results are as shown in Table 3 below.

TABLE 3

Free Radical Scavenging Activity of Geummo Gochu Extracts and Existing Antioxidants

IC ₅₀ of Examples 4-7 and 11-13 including Examples 8 and 14 were relatively low. Although these extracts have been shown to have high activity comparable to conventional antioxidants, they are crude extracts.

Experimental Example 3 Inhibitory Effects of Geummo Gochu Extracts on Hydrogen Peroxide-induced Cytotoxicity

The inhibitory effect on the hydrogen peroxide-induced cytotoxicity of the gold hair extract extract prepared in Example 6 was measured using mouse-derived fibroblasts.

Hydrogen peroxide produces hydroxyl radicals in cells, causing lipid peroxidation, leading to cell membrane damage and cytotoxicity. The free radical scavenging activity of the extracts of gold hairs was measured as follows to test at the skin cell level.

Mouse skin fibroblasts of the logarithmic growth stage were inoculated into a 96 well plate at 10,000 cells per well, incubated in 80 µl of DMEM medium for 24 hours, and then diluted in the medium so that the final concentration in the medium was 10 ^-3 M. 10 μl of hydrogen peroxide was added and 10 μl of the dimethylsulfoxide solution of the gold hair extract of Example 6 was added at a final concentration of 0, 50, 100, 200, 500 μg / ml, followed by further incubation for 20 hours, and then 5 mg / ml. 15 μl of the MTT solution was added thereto, and after 4 hours, 150 μl of the dimethyl sulfoxide solution was added and dissolved, and then the absorbance was measured at 570 nm using a microplate reader.

The inhibitory effect on the hydrogen peroxide-induced cytotoxicity of the extract of Geummo chuck was calculated by the following formula.

The results are as shown in Table 4 below.

TABLE 4

Inhibitory Effects of Geummo Gochu Extracts on Hydrogen Peroxide-induced Cytotoxicity

* 3 times average

The concentration of gold moth extract was suppressed concentration dependently on hydrogen peroxide-induced cytotoxicity. Therefore, it can be seen that the extract of gold hair chuck suppresses cytotoxicity by inhibiting lipid peroxidation generated in mouse-derived fibroblasts.

Experimental Example 4 Cytotoxicity Experiment

Cytotoxicity of the hair follicle extract itself prepared in Example 6 was measured using the dermal fibroblasts derived from the mouse as follows.

After inoculating 10,000 mouse skin fibroblasts of the logarithmic cell per well into a 96-well plate and incubating for 24 hours in 80 μl of DMEM medium, 10 μl of the dimethyl sulfoxide solution of the gold moth extract of Example 6 was finalized. Was added to 0, 50, 100, 200, 500 ㎍ / ml, and further incubated for 20 hours, and then 15 µl of 5 mg / ml MTT solution was added and after 4 hours, 200 µl of dimethyl sulfoxide solution was added and dissolved. The absorbance was then measured at 570 nm with a microplate reader.

Cytotoxicity of the chymococcal extract itself was expressed by cell viability and was calculated by the following formula.

The results are as shown in Table 5 below.

TABLE 5

Cytotoxicity

* 3 times average

The hair follicle extract of Example 6 did not show significant cytotoxicity in itself as compared to the negative control group.

Prescription Example 1

The following is an example of prescription of flexible cosmetics in cosmetics containing extract of Geumgumcheok.

Here, the gold moth chuck extract is that of Example 6.

Prescription Example 2

The following is an example of prescription of astringent cosmetics in cosmetics containing extract of Geumgumcheok.

Here, the gold moth chuck extract is that of Example 6.

Prescription Example 3

A prescription example of nutrient cosmetic lotion (lotion) in cosmetics containing the extract of Geummo chuck is as follows.

Here, the gold moth chuck extract is that of Example 6.

Prescription Example 4

The following is an example of prescription of nourishing cream in cosmetics containing extract of Geumgumcheok.

Here, the gold moth chuck extract is that of Example 6.

Prescription Example 5

A prescription example of a massage cream in cosmetics containing the extract of Geumgumcheok is as follows.

Here, the gold moth chuck extract is that of Example 6.

Prescription Example 6

Prescription examples of essences in cosmetics containing the extract of Geumgumcheok are as follows.

Here, the gold moth chuck extract is that of Example 6.

Prescription Example 7

A prescription example of a pack in cosmetics containing the extract of Chalmo chuck is as follows.

Here, the gold moth chuck extract is that of Example 6.

The free radical scavenging cosmetic of the present invention prevents or delays aging of the skin by suppressing oxidation of a biological material by free radicals by eliminating free radicals of the skin.

Claims (6)

A free radical scavenger cosmetic containing Cibotium barometz extract. 2. The cosmetic for free radical scavenging according to claim 1, wherein the hair follicles are cibotii Rhizoma . According to claim 1, Free radical scavenging cosmetics, characterized in that it contains 0.001 to 10% by weight of the extract of gold hair chuck. The method for free radical scavenging according to claim 1, wherein the extract of chamomile extract is obtained by drying the root diameter of chamomile extract and making it into fine powder, and then extracting the extracted solution by immersion at room temperature for 1 to 15 days by adding an extraction solvent. Cosmetics. The free radical scavenging cosmetic according to claim 4, wherein the extraction solvent is selected from the group consisting of water, lower alcohol, a mixed solvent of water and lower alcohol, acetone, diethyl ether, chloroform, hexane and ethyl acetate. The cosmetic for free radical scavenging according to claim 1, which is selected from the group consisting of softening cream, converging cream, nutrient cream, nourishing cream, massage cream, essence and pack.