KR100787782B1 - Cosmetic composition for reducing the skin irritation comprising the extract of nymphaea caerulea as active ingredient - Google Patents

Abstract

A cosmetic composition comprising an extract of Nymphaea caerulea is provided to alleviate the skin irritation significantly and secure increased anti-inflammatory activity, thereby providing excellent skin irritation-reducing effect when being applied to cosmetics. A cosmetic composition for reducing skin irritation comprises 0.0001-20.0 wt.% of an extract of Nymphaea caerulea as an effective ingredient, wherein the extract is extracted by using a solvent selected from the group consisting of water, C1-4 alcohol, acetone, ethyl acetate, chloroform, 1,3-butyleneglycol and butyl acetate. The composition is in the form of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray.

Description

Cosmetic Composition for Reducing the Skin Irritation Comprising the Extract of Nymphaea caerulea as Active Ingredient}

The present invention relates to a cosmetic composition for alleviating skin irritation containing a blue lotus extract.

In general, many skin-related products contain substances that can cause irritation or inflammation, and more than 10% of people who use personal care products complain of side effects on their skin. There is a growing number of consumers who feel that skin is sensitive. More than 30-40% of respondents say they are sensitive skin in a consumer survey, and this trend continues to increase. Moreover, recent cosmetics are focused on functional cosmetics (cosmeceutical) that can have a real effect on the skin, so the skin side effects due to the use of personal care products such as cosmetics is expected to increase gradually. In particular, the environmental pollution is severe and the skin is easily damaged due to the increase in the amount of ultraviolet radiation due to the destruction of the ozone layer, the drying of the living space, the constitution of the individual's allergies, the stress, the chemical harmful substances, and the like. Therefore, the skin becomes easily reddened or sensitive.

Generally, various raw materials are used to make cosmetics, and usually 20 to 50 raw materials are used. These ingredients have been proven to be safe through skin irritation tests, but they can be applied to products that can cause irritation, itching and allergies to the skin. Skin irritation that may occur with the use of cosmetics includes irritation such as itching, stinging and burning, and erythema and edema. Contact dermatitis may be roughly classified into irritating contact dermatitis, allergic contact dermatitis, sensory stimulation and phototoxic dermatitis, but it is difficult to clearly distinguish them.

Recently, many efforts to protect the skin from external stimuli have been progressed variously in cosmetic companies, etc. In particular, various raw materials are being developed using natural products.

Therefore, the main object of the present invention is to provide an excellent new material to alleviate skin irritation caused by external stimuli and skin problems caused by high functional cosmetic raw materials.

Another object of the present invention to provide a cosmetic composition comprising a substance having an excellent skin irritation relief effect as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

According to one aspect of the present invention, the present invention provides a cosmetic composition for alleviating skin irritation comprising a blue lotus extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The blue lotus ( Nymphaea caerulea) used in the present invention is a perennial herb belonging to the water lily family, and its origin is South Africa, and is a wild plant with leaves of about 30 cm in diameter, and grows on islands in southern and eastern Asia. Called blue lotus, blue lotus, and dahlia, the plant's name comes from blue flowers, which are known to contain anti-aging, antioxidants and inhibit free radicals.

In the cosmetic composition of the present invention, the blue lotus extract means obtained by extracting from various organs such as water lilies and leaves, stems, roots, flowers, seeds of plants and plants, and preferably means extracts obtained from leaves or flowers. do.

The blue lotus extract of the present invention can be prepared according to conventional methods known in the art, that is, using conventional solvents, under conditions of conventional temperature and pressure.

The blue lotus extract can be prepared in various extraction solvents, such as water, anhydrous or hydrous lower alcohols (methanol, ethanol, propanol and butanol) having 1 to 3 carbon atoms, acetone, ethyl acetate, chloroform, 1,3-butylene glycol and Obtained as an extraction solvent selected from the group consisting of butyl acetate, preferably obtained by using a suitable mixture of water and ethanol, more preferably using a mixed solution of 3: 7 mixed with water and ethanol Obtained. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvents, extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the extract of the present invention.

The content of the blue lotus extract of the present invention is 0.0001-20.0% by weight based on the total weight of the cosmetic composition, preferably 0.001-10.0% by weight. If the content is less than the expected effect is difficult to appear, if the content is exceeded the effect is no longer increased.

In the present invention, in order to alleviate the skin irritation by applying a blue lotus extract to the cosmetics in order to alleviate the skin irritation caused by the skin irritation caused by external stimulation and cosmetic raw materials.

In the present invention, the term "skin irritation relief" refers to preventing, alleviating and treating inflammatory irritation reactions such as itching, tingling, burning and erythema and swelling caused by skin irritation substances and appearing on the skin. . In particular, the cosmetic containing blue lotus extract of the present invention has a very excellent feature of the skin irritation relief effect (Reference Experimental Examples 1 to 5). Therefore, the blue lotus extract of the present invention can be used as a cosmetic adjuvant to relieve skin irritation to cosmetic raw materials.

Raw material causing skin irritation in the cosmetic composition includes a natural extract and ingredients that can be commonly used in cosmetics. Representative ingredients causing skin irritation include, but are not limited to, lactic acid, glycolic acid, salicylic acid, sodium lauryl sulfate, and retinoic acid. no.

The cosmetic composition of the present invention is prepared as a composition containing a blue lotus extract as an active ingredient, and may be applied before or after applying a cosmetic composition having a large skin irritation to the skin. In addition, blue lotus extract may be applied to the skin by additionally added to the cosmetic composition with a large skin irritant.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the blue lotus extract as active ingredients, and include conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

Skin irritation cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, interfaces It may be formulated with an active agent-containing cleansing, but is not limited thereto. More specifically, in the formulation of flexible lotion, nutrition lotion, milk lotion, nutrition cream, massage cream, essence, eye cream, cleansing lotion, cleansing cream, cleansing foam, cleansing water, powder wash, cleansing oil, mask, pack Can be prepared.

When the formulation of the present invention is a paste, cream or gel, the carrier component is animal oil, vegetable oil, ester oil, wax, paraffin, starch, tracant, cellulose derivative, carbomer, polyacrylamide, polyethylene glycol, silicone, Bentonite, silica, talc or zinc oxide and the like can be used.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylene glycol, oils, glycerol aliphatic esters, polyethylene glycols or sorbitan.

When the dosage form of the present invention is a suspension, a carrier diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are intended to illustrate the present invention in more detail, and the scope of the present invention is not limited by these examples. In addition, the part,%, and ratio used in this Example correspond to a mass reference | standard unless there is particular notice.

Preparation Example 1 Preparation of Blue Lotus Extract I

First, the leaves and flowers of the blue lotus are dried and shredded to weigh 100 grams (g). 600 g of 70% ethanol is added thereto and soaked for 5 days to extract the active ingredient. After extracting for 5 days, the resultant was filtered with a 300 mesh filter cloth, filtered with a filter paper of Whatman 5, and dried with a rotary vacuum evaporator to obtain a dry weight of 7.5 grams (g). The dried powder was dissolved in 30% 1,3-butylene glycol to prepare a blue lotus extract, which was used in the present invention.

Preparation Example 2-11. Preparation of Blue Lotus Extract II

First, dry the blue lotus leaves and flowers, then shred them finely to weigh 100 grams. Here, 600 grams (g) of the extraction solvent of Table 1 was added thereto, and soaked for 5 days to extract the active ingredient. After extracting for 5 days, filtered through a 300 mesh filter cloth, again filtered with a filter paper Whatman 5, and dried with a rotary vacuum evaporator, to obtain a dry weight as shown in Table 1.

Manufacturing example number Extraction solvent (ethanol) Dry weight (g) 2 0% 5.23 3 10% 5.68 4 20% 6.13 5 30% 6.12 6 40% 6.23 7 50% 6.45 8 60% 6.13 9 80% 7.65 10 90% 7.56 11 100% 7.35

Preparation Example 12 Preparation of Blue Lotus Extract III

First, dry the blue lotus leaves and flowers, then shred them finely to weigh 100 grams. Add 600 grams of a solvent mixed with water and 1,3-butylene glycol in a 5: 5 (v: v) solution, and immerse for 5 days to extract the active ingredient. After extracting for 5 days, filtered through a 300 mesh filter cloth, again filtered with a filter paper Whatman 5, to obtain 580g of blue lotus extract. The dry weight at this time is 12.5 g / 100 ml.

Experimental Example 1. Test of cell stimulation relaxation effect by lactic acid of blue lotus extract

Fibroblasts (Korea Cell Line Bank, South Korea), which are human skin cells, were cultured in T-75 flasks (Falcon, United States) until they grew about 80%. This was again transferred to 96 × well plates (Falcon, USA) to 3 × 10 ⁴ cells / well and incubated for 24 hours. After culturing, the microscope confirmed whether the cells were completely attached and grew well, and the experiment was performed using lactic acid, a skin cell stimulating source. At this time, lactic acid was used at 0.2% (all skin cells were killed at this concentration). That is, 200 μl of DMEM (Sigma, United States) medium containing 0.2% lactic acid was added to each of 96 wells, and 0.1, 0.5, 1.0, 2.0, 5.0% (v / adjusted to v). At this time, no lactic acid and extract were added to the control group, only the lactic acid was added as a comparative group, and also no lactic acid was added at each concentration to check whether the blue lotus extract was irritated. In order to compare the survival rate of the cells after 12 hours, MTT solution (Sigma, United States; 3mg / ml) was added to measure the cell viability using ELISA READER (Molecular Devices, USA). Cell viability (%) was calculated according to Equation 1 below, and the results are shown in Table 2 below.

 Cell survival rate according to the concentration of blue lotus extract (%) I Blue Lotus Extract Concentration (%) Cell survival rate (%) Without lactic acid With lactic acid 0.0 100.0 0.0 0.1 99.8 13.5 0.5 98.8 25.7 1.0 101.3 35.6 2.0 102.6 56.4 5.0 100.4 78.9

Through the results of Table 2, through the results of Table 2, it was found that the blue lotus extract inhibits cell death by lactic acid, depending on the concentration of the addition. In addition, when only the blue lotus was added, it was found that the skin irritation caused by the blue lotus extract was not induced at all, even though the concentration had little effect on the cell viability.

Experimental Example 2. Retinol-induced cell stimulating effect test of blue lotus extract

Fibroblasts (Korea Cell Line Bank, South Korea), which are human skin cells, were cultured in T-75 flasks (Falcon, United States) until they grew about 80%. This was again transferred to 96 3 wells (Falcon, USA) to 3 × 10 ⁴ cells / well and incubated for 24 hours. After culturing, the microscope confirmed whether the cells were completely attached and grew well. The experiment was conducted using retinol 0.005% (all skin cells are killed at this concentration) as a skin cell stimulant. That is, 200 μl of DMEM (Sigma, USA) medium containing 0.005% retinol was added to each of 96 wells, and 0.1, 0.5, 1.0, 2.0, 5.0% (v / v) was prepared using the Blue Lotus extract of Preparation Example 1. Adjusted to). At this time, no retinol and extracts were added to the control group, and only retinol was added to the comparison group. In addition, to confirm the irritation of the blue lotus extract was also used without adding lactic acid at each concentration. Cell viability was measured by adding MTT solution (Sigma, United States; 3 mg / ml) to compare the viability of the cells after 12 hours after the addition of the test substance. Cell viability was measured by absorbance at 570nm using an ELISA READER (Molecular Devices, United States of America), and calculated the cell viability (%) according to Equation 1, the results are shown in Table 3 below.

Cell survival rate (%) according to the concentration of blue lotus extract II Blue Lotus Extract Concentration (%) Cell survival rate (%) Without retinol With retinol 0.0 100.0 0.0 0.1 99.8 5.6 0.5 98.8 12.6 1.0 101.3 24.8 2.0 102.6 45.8 5.0 100.4 65.8

Through the results in Table 3, it was found that the blue lotus extract inhibits cell death by retinol depending on the concentration. In addition, when only the blue lotus was added, it was found that the skin irritation caused by the blue lotus extract was not induced at all, even though the concentration had little effect on the cell viability.

Experimental Example 3. Cytotoxic effect of blue lotus extract by sodium lauryl sulfate

Fibroblasts (Korea Cell Line Bank, South Korea), which are human skin cells, were cultured in T-75 flasks (Falcon, United States) until they grew about 80%. This was again transferred to 96 3 wells (Falcon, USA) to 3 × 10 ⁴ cells / well and incubated for 24 hours. After incubation, the cells were completely attached and grown well under a microscope. Sodium Lauryl Sulfate (Sigma, U.S.A .; abbreviated as 'SLS'), a skin cell stimulant, was used. At this time, SLS was used at 0.002% (all the skin cells were killed at this concentration). That is, 200 μl of DMEM (Sigma, United States) medium containing 0.2% lactic acid was added to each of 96 wells, and 0.1, 0.5, 1.0, 2.0, 5.0% (v / v) using the composition prepared in Preparation Example 1. Adjusted to add. At this time, SLS and blue lotus extract were not added to the control group, and only SLS was added as a comparison group, and also in the group without adding SLS at each concentration of the blue lotus extract to confirm whether the blue lotus extract was irritated. The experiment was conducted. After 12 hours after the addition of the test substance, cell viability was measured by adding MTT solution (Sigma, United States; 3mg / ml) to compare the cell viability. Cell viability was measured by absorbance at 570nm using an ELISA READER (Molecular Devices, United States of America) and calculated the cell viability (%) according to Equation 1, the results are shown in Table 4 below.

Cell survival rate according to the concentration of blue lotus extract (%) III Blue Lotus Extract Concentration (%) Cell survival rate (%) Without SLS With SLS 0.0 100.0 0.0 0.1 99.8 25.6 0.5 98.8 45.8 1.0 101.3 65.8 2.0 102.6 78.9 5.0 100.4 89.6

Through the results of Table 4, it was found that the blue lotus extract inhibits cell death by SLS depending on the concentration. In addition, when only the blue lotus was added, it was found that the skin irritation caused by the blue lotus extract was not induced at all, even though the concentration had little effect on the cell viability.

Experimental Example 4. Anti-inflammatory effect of blue lotus extract-Karakinan edema experiment

Carrageenan foot edema in order to determine whether the Blue Lotus extract of the present invention has an anti-inflammatory effect The experiment was performed. The blue lotus extract obtained in Preparation Example 1 was administered to the abdominal cavity at a dose of 10-100 mg / kg, respectively, and 1 hour later, 0.5 ml of 0.1% carrageenan solution was injected into the rear sole of the experimental animal to induce inflammation. The change in paw volume of rats immediately after carrageenan injection and 4 hours after injection was measured, and the edema inhibition rate was calculated according to Equation 2 below, and the results are shown in Table 5 below.

ΔV in the above equation is the change in foot volume.

Edema inhibition rate (%) Blue Lotus Dose (mg) Edema inhibition rate (%) 10 12.6 20 25.3 50 43.2 100 65.8

As shown in the results of Table 5 above, it was found that the swelling inhibition effect was increased depending on the dose of the blue lotus extract through the Karakinan edema experiment, and therefore, the anti-inflammatory effect by the blue lotus was increased. Can be.

Experimental Example 5 Skin Safety Comparison

In order to compare the skin stability of the cosmetics containing the blue lotus extract, the cosmetic composition containing the blue lotus extract prepared in Preparation Example 1 was prepared in the composition of Table 6 below.

Cosmetics containing blue lotus extract and irritant (content-% by weight) ingredient Control Comparison Example 1 Example 2 Comparative Example 1 Comparative Example 2 Blue lotus - 3.0 3.0 3.0 - - AHA - - 5.0 - 5.0 - Retinol - - - 0.1 - 0.1 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 4.0 4.0 4.0 Liquid paraffin 4.6 4.6 4.6 4.6 4.6 4.6 Triethanolamine 1.0 1.0 1.0 1.0 1.0 1.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 Macadamia Nut Oil 2.5 2.5 2.5 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 1.6 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 1.6 1.6 1.6 1.6 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount system 100 100 100 100 100 100

Skin stimulation experiments were carried out on 20 women in their 20s to 30s with the cosmetic compositions of Comparative Examples, Comparative Controls, Examples 1 and 2, and Comparative Examples 1 and 2, which were prepared with the composition of Table 6 above. The subjects were patched to the subject's upper arm using the cosmetic composition and checked for skin irritation after 24 hours.

Skin irritation effect Skin reaction degree Control Comparative Example Example 1 Example 2 Comparative Example 1 Comparative Example 2 No erythema 10 15 12 13 - - Very slight erythema 10 5 7 7 2 4 Clear erythema - - One - 18 16 Severe erythema - - - - - -

As shown in the results of Table 7, blue lotus extract showed a very high effect in relieving skin irritation by AHA and retinol, especially in the general formulation without any treatment. This shows that blue lotus extract has a significant effect on reducing skin irritation caused by cosmetic ingredients.

Hereinafter, a formulation example of a cosmetic composition containing a blue lotus extract, for example, will be described in detail, but the composition of the present invention is not limited to these formulations.

Formulation Example 1 Softener (Skin Lotion)

Formulation example of the flexible longevity containing the blue lotus extract obtained by the method of Preparation Example 1 was prepared as shown in Table 8 in accordance with a conventional method.

ingredient Content (% by weight) Blue lotus extract 0.1 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2. Nutrients (Milk Lotion)

Formulation example of nutrient lotion including the blue lotus extract obtained by the method of Preparation Example 1 was prepared as shown in Table 9 in accordance with a conventional method.

ingredient Content (% by weight) Blue lotus extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3. Nutrition Cream

Formulation example of the nutrition cream containing a blue lotus extract obtained by the method of Preparation Example 1 was prepared as shown in Table 10 in accordance with a conventional method.

ingredient Content (% by weight) Blue lotus extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4 Massage Cream

Formulation example of the massage cream containing a blue lotus extract obtained by the method of Preparation Example 1 was prepared as shown in Table 9 according to a conventional method.

ingredient Content (% by weight) Blue lotus extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5 Pack

Formulation example of a pack containing a blue lotus extract obtained by the method of Preparation Example 1 was prepared as shown in Table 12 in accordance with a conventional method.

ingredient Content (% by weight) Blue lotus extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

As described in detail above, the present invention provides a cosmetic composition for alleviating skin irritation comprising the blue lotus extract as an active ingredient. Blue lotus extract of the present invention significantly relieve skin irritation caused by cosmetic raw materials compared to the existing anti-inflammatory agent, and also exhibits increased anti-inflammatory activity, when used in cosmetics can be expected to excellent skin irritation effect. Therefore, since the cosmetic composition of the present invention can alleviate the skin irritation that may occur when the cosmetic is applied to the skin using the blue lotus extract, the development of the cosmetic composition without side effects on the skin and the skin safety of the cosmetic may be improved. Can be.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (4)

A cosmetic composition for alleviating skin irritation comprising blue lotus ( Nymphaea coerulea) extract as an active ingredient.       The cosmetic composition according to claim 1, wherein the blue lotus extract is 0.0001-20.0 wt% based on the total weight of the cosmetic. The method of claim 1, wherein the blue lotus extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, 1,3-butylene glycol and butyl acetate Cosmetic composition, characterized in that extracted using an extraction solvent selected from the group consisting of. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition, characterized in that it has a formulation selected from the group.