KR20010018658A - A cosmetic composition containing Evodiae Fructus extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Evodiae Fructus is prepared for use as sunblock, which is excellent in scavenging free radicals, absorbing ultraviolet ray without allergy. CONSTITUTION: In the cosmetic composition, Evodiae Fructus is extracted from Evodiae officinalis Dode, and 0.001-10 wt.% of Evodiae Fructus extract is incorporated into cosmetic composition. Evodiae Fructus is extracted by using one solvent selected from the group consisting of water, methanol, ethanol, acetone, ethyl acetate, 1,3-buthylene glycol, hexane, diethyl ether, normal propanol. iso-propanol, and normal butanol.

Description

A cosmetic composition containing Evodiae Fructus extracts

The present invention relates to a cosmetic composition containing an extract of Evodiae Fructus extracted from a fruit of Unhyang (식물 香) and the plant of Evodiae officinalis Dode.

Modern people are exposed to harmful environments such as air pollution, unhealthy eating, UV exposure, stress, and disease, making it difficult to sufficiently eliminate oxidative stress caused by various free radicals. This oxidative stress causes problems such as skin aging.

Free radicals are elements or molecules with one or more unpaired electrons and have the property of stabilizing electrons from other molecules. Free radical life is very short (a few thousandths of a second), unstable and very aggressive.

For this reason, a lot of researches have been made since the development of a safer and high free radical scavenging effect has emerged as an important task in the cosmetic field.

Therefore, the present inventors conducted an exploration study on herbal medicines in Korea to develop free radical scavenging substances from plants, and found that the extract of Evodia Fructus was very excellent in free radical scavenging effect. To complete.

Therefore, an object of the present invention is to provide a cosmetic composition containing a sewage extract.

The present invention relates to a cosmetic composition containing the extract of Evodiae Fructus.

The sewage oil extract characteristicly contained in the cosmetic composition according to the present invention is extracted from the fruit of the fragrance and the plant of the sewage (Evodia officinalis Dode). Osuyu is originated from China and imported as medicinal water and grows in 50m above sea level in Gyeongsangbuk-do. It is a deciduous arborescent and is 5m high and has hairs on young branches. In autumn, mature fruits are collected and dried in the sun. The sewage fruit has the shape of spherical to spherical shape and is 2.5mm to 5mm in diameter. The outer surface is dark brown to greyish brown, and there are many concave small points due to the loss, and there are residues of shipper, but are often separated. Fruits are 2mm to 5mm long, dense greenish hairs. The rind is divided into 5 threads in the mature one, and each stall contains obovate or spherical brown to blackish brown or blueish black shiny seeds.

The sewage oil extract contained in the composition according to the present invention is prepared from the fruit of powdered sewage oil (Evodiae officinalis Dode) from water, methanol, aqueous methanol solution, ethanol aqueous ethanol solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3 -Butylene glycol aqueous solution, hexane, diethyl ether, normal propanol, iso-propanol or normal butanol can be extracted using an extraction solvent and concentrated under reduced pressure.

The sewage oil extract may be prepared by immersion for 1 to 15 days at 15 ° C. to 40 ° C. under the solvent or after extraction for 1 to 7 days in an extractor equipped with a cooling capacitor, followed by concentration under reduced pressure. In addition, the extraction process may include a low-temperature aging process for 7 to 10 days at 4 to 15 ℃.

In the preparation method of the sewage oil extract, the extraction solvent is preferably used in an amount of 1 to 15 times the dry weight of the sewage fruit.

The sewage oil extract prepared as described above may be prepared as a cosmetic composition by a conventional method by adding a conventional additive, and preferably contained in an amount of 0.001 to 10 weights, more preferably 1 to 7 weights, based on the amount of the cosmetics dried. .

The cosmetic composition according to the present invention may be prepared in the form of a basic cosmetic such as flexible lotion, milk lotion, nourishing cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil.

In addition, the cosmetic composition according to the present invention can be prepared in the form of color cosmetics, such as foundation, lipstick, mascara or makeup base.

In addition, the cosmetic composition according to the present invention can be prepared in the form of a hair cosmetic such as shampoo, conditioner, hair conditioner or hair gel.

Hereinafter, the present invention will be described in more detail with reference to Examples, Experimental Examples, Comparative Examples, and Prescription Examples, but the present invention is not limited thereto.

Example 1

After washing with purified water and drying, 1 kg of powdered sewage oil was poured into 5 L of water, extracted at about 20 ° C. for 5 days, filtered through 300 mesh filter cloth, left at 10 ° C. for 10 days, and then aged at low temperature. Filtered. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 58.9 g (dry weight).

Example 2

After washing with purified water and drying, 1 kg of powdered sewage oil in 5 liters of 10 ethanol was extracted in an extractor equipped with a cooling condenser for 3 days, filtered through a 300 mesh filter cloth, left at 10 ° C. for 10 days, and then aged at low temperature. Was filtered. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 71.8 g (dry weight).

Examples 3 to 21

To extract the dry weight sewage oil extract as described in Table 1 using the solvent of Table 1 to extract the method of Example 2.

division menstruum Dry weight (unit: g) Example 3 20 Ethanol 75.6 Example 4 30 Ethanol 78.8 Example 5 40 Ethanol 89.6 Example 6 50 ethanol 94.8 Example 7 60 Ethanol 100.2 Example 8 70 Ethanol 105.2 Example 9 80 Ethanol 103.1 Example 10 90 Ethanol 92.2 Example 11 100 Ethanol 78.6 Example 12 80 Methanol 112.1 Example 13 100 Methanol 74.2 Example 14 Acetone 27.6 Example 15 Ethyl acetate 15.7 Example 16 501,3-butylene glycol aqueous solution 89.8 Example 17 Hexane 15.6 Example 18 Diethyl ether 19.6 Example 19 Normal propanol 26.5 Example 20 Isopropanol 28.6 Example 21 Normal butanol 37.8

Experimental Example 1

Free radical scavenging effect of sesame oil extract

1) Experiment Method

The free radical scavenging effect was found in dimethyl sulfoxide (SEO) extract of sesame oil in 2 ml of 60 μM 1,1-diphenyl-2-picryl hydrazyl (DPPH) ethanol solution. 2 ml of DMSO) solution was added and reacted at room temperature for 30 minutes, and then the absorbance was measured at 520 nm to determine the free radical scavenging effect (). The free radical scavenging effect of each example extract was calculated by the following formula.

Free radical scavenging effect () = 100-[(absorbance / control absorbance after extract treatment) × 100]

2) Experiment result

Free radical scavenging effect of the sesame oil extract is shown in Table 2. Most of the sesame oil extracts showed high activity.

Sewage Extract Free radical scavenging effect () Experimental concentration (㎍ / ㎖) 5 25 50 Example 1 10.5 45.5 65.5 Example 2 14.5 54.2 76.3 Example 3 13.2 55.3 78.9 Example 4 14.6 55.5 82.9 Example 5 18.5 58.9 88.9 Example 6 17.6 59.6 91.5 Example 7 17.2 58.8 92.3 Example 8 18.5 57.9 94.6 Example 9 19.2 60.8 93.6 Example 10 17.8 62.5 92.8 Example 11 17.4 62.3 93.2 Example 12 20.1 62.1 95.1 Example 13 18.8 59.8 94.3 Example 14 21.5 67.3 90.2 Example 15 22.3 68.6 94.6 Example 16 15.5 54.9 87.5 Example 17 10.8 37.4 57.6 Example 18 11.9 26.3 53.8 Example 19 16.8 47.6 71.2 Example 20 19.1 48.2 80.2 Example 21 15.3 54.5 87.6

* The above value is the average of 3 runs

Comparative Example 1

The sesame oil extract used was prepared in Example 12. The free radical scavenging effect between the sesame oil extract and the existing scavengers was measured in the same manner as in Experimental Example 1, and the results of comparing the SC ₅₀ values with the SC ₅₀ are shown in Table 3. SC ₅₀ is the concentration of sample required to remove 50 free radicals.

Test substance SC ₅₀ (μg / ml) Sewage Extract (Example 12) 22.16 Ascorbic acid 29.74 Dibutyl hydroxyl toluene (BHT) 38.05 DL-α-Tocopherol 34.67 Beta-carotene 54.01 Morin 23.61

Comparing the free radical scavenging effect of the sesame oil extract with conventional scavenger, the sesame oil extract showed very high activity.

Experimental Example 2

Inhibition of Hydrogen Peroxide (H ₂ O ₂ ) -induced Cytotoxicity of Sewage Extract

The inhibitory effect on the hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity of the sesame oil extract was measured using mouse-derived skin fibroblasts. Hydrogen peroxide generates hydroxyl radicals in the cell, causing fatal damage to the cell. The free radical scavenging effect of the sesame oil extract was measured as follows to test at the cellular level.

1) Experiment Method

The sesame oil extract used was prepared in Example 12. 5 × 10 ⁴ mouse-derived fibroblasts (fibroblast, NIH / 3T3) were inoculated into 96 well plates in 5 × 10 ⁴ cells in each well, and cultured in 200 μl of Dulbecco's Modified Eagle Medium (DMEM) for 24 hours. H ₂ O ₂ diluted to a final concentration of 1 mM was added, and at the same time, a 1,3-butylene glycol (50v / v, aqueous solution) solution of sewage oil extract was added at a final concentration of 0, 10, 50, 100, 200 µg / ml. After incubation for 24 hours, 10 μl of MTT solution (tetrazolium salt 3- (4,5-Dimethylthiazolyl-2-yl) -2,5-diphenyl tetrazolium bromide) was added per well, and left for 4 hours. . 100 μl of dimethyl sulfoxide (DMSO) solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.

2) Experiment result

Table 4 shows the inhibitory effect of hydrogen peroxide extract on hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity. The cytotoxic effect was calculated by the following formula.

Cytotoxic inhibitory effect () = [(absorbance at extract treatment-absorbance at H ₂ O ₂ treatment) / absorbance at H ₂ O ₂ treatment] × 100

Concentration of Sewage Oil Extract (µg / mL) Cytotoxic effect () 0 0 10 5.02 50 20.45 100 30.55 200 41.21

* 3 times average value

As shown in the above results, sewage extract showed concentration-dependent inhibition of hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity. Therefore, the sesame oil extract suppressed cytotoxicity by scavenging hydroxyl radicals generated in fibroblasts.

Experimental Example 3

Cytotoxicity of Sewage Extract

Cytotoxicity of the sesame oil extract itself was measured using mouse-derived skin fibroblasts (NIH / 3T3).

1) Experiment Method

The sesame oil extract prepared in Example 12 was used.

5 × 10 ⁴ mouse-derived fibroblasts were inoculated into 96 well plates, incubated in 200 μl of DMEM medium, and then the final concentration of the effluent extract solution was 0, 50, 100, 250, 500 μg / ml. Added and incubated for 24 hours. After 10 μl of MTT solution was added and left for 4 hours, 100 μl of dimethyl sulfoxide (DMSO) was added to the wells, dissolved for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.

2) Result of experiment

The cytotoxicity of the sesame oil extract itself is shown in Table 5. Cytotoxicity was calculated according to the following formula as cell viability.

Cell survival rate () = (absorbance at extract treatment / absorbance of control) × 100

Concentration of Sewage Oil Extract (µg / mL) Cell viability () 0 100 50 100.45 100 95.71 250 91.9 500 87.7

* 3 times average

Prescription Example 1

Formulation examples of the flexible lotion among cosmetics containing sesame oil extract are shown in Table 6.

ingredient content Sewage Extract 5.0 1,3-butylene glycol 6.0 Sodium hyaluronate 2.0 glycerin 4.0 Fiji 4000 1.0 Poly Solvent 20 0.5 ethanol 10.0 antiseptic Quantity Benzophenone-9 0.05 incense Quantity Purified water Quantity Sum 100

Prescription Example 2

A prescription example of milk lotion in cosmetics containing sesame oil extract is as follows.

ingredient Content (weight) Sewage Extract 5.0 Stearic acid 0.4 1,3-butylene glycol 6.0 Cetostearyl alcohol 1.2 glycerin 4.0 Glyceryl Stearate 1.0 Triethanol amine 0.25 Tocopheryl acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Poly Solvent 60 1.5 Solbitan Sesquioleate 0.6 Carboxyvinyl Polymer 0.15 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 3

A prescription example of a nourishing cream in cosmetics containing sesame oil extract is as follows.

ingredient Content (weight) Sewage Extract 5.0 vaseline 7.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 2.0 Stearic acid 1.5 Floating paraffin 10.0 Beeswax 2.0 Poly Solvent 60 1.5 Solbitan Sesquioleate 0.8 Squalane 3.0 1,3-butylene glycol 6.0 glycerin 4.0 Triethanol amine 0.5 Cotoperyl acetate 0.1 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 4

Prescription examples of essences in cosmetics containing sesame oil extract are as follows.

ingredient Content (weight) Sewage Extract 5.0 glycerin 10.0 Fiji 1500 2.0 Allantoin 0.1 Panthenol 0.3 EDTA 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octyldodeceth-25 0.6 ethanol 6.0 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

Prescription Example 5

A prescription example of a massage cream in cosmetics containing a sesame oil extract is as follows.

ingredient Content (weight) Sewage Extract 3.0 Glyceryl Stearate 2.0 Cetostearyl alcohol 2.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 Squalene 5.0 Mineral oil 35.0 Dimethicone 1.0 Xanthan Gum 0.1 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanol amine 0.5 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

Prescription Example 6

Examples of prescription packs in cosmetics containing sesame oil extract are as follows.

ingredient Content (weight) Sewage Extract 3.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 Fiji 1500 2.0 Panthenol 0.4 Allantoin 0.1 ethanol 6.0 Fiji 40 Cured Castor Oil 0.3 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

As can be seen from the above, the sewage oil extract according to the present invention not only has an excellent free radical scavenging effect, but also has little cytotoxicity, and thus has excellent safety, and thus can be effectively used in cosmetic compositions.

Claims (7)

Cosmetic composition containing the extract of Evodiae Fructus. The cosmetic composition according to claim 1, wherein the sewage oil extract is contained in an amount of 0.001 to 10% by weight based on the dry weight of the cosmetic. The process according to claim 1, wherein the effluent oil extract is water, methanol and methanol aqueous solution, ethanol and ethanol aqueous solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butylene glycol aqueous solution, hexane, diethyl ether, normal Cosmetic composition, characterized in that extracted with an extraction solvent selected from the group consisting of propanol, iso-propanol and normal butanol. The cosmetic composition according to claim 1, which is a basic cosmetic, color cosmetic or hair cosmetic. 5. The cosmetic composition according to claim 4, wherein the basic cosmetic is softener, milk crocion, nutrition cream, massage cream, essence, cleansing foam, cleansing water, pack, or body oil. The cosmetic composition according to claim 4, wherein the color cosmetic is a foundation, lipstick, mascara or makeup base. The cosmetic composition according to claim 4, wherein the cosmetic for hair is a shampoo, a rinse, a hair conditioner or a hair gel.