KR20010018667A - A cosmetic composition coantaining Artemisiae capillaris Herba extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Artemisiae capillaris Herba extract is prepared for use as sunblock, which is excellent in scavenging free radicals, absorbing ultraviolet ray without allergy. CONSTITUTION: In the cosmetic composition, 0.001-10 wt.% of Artemisiae capillaris Herba extract is incorporated into cosmetic composition. Artemisiae capillaris Herba is extracted by using one solvent selected from the group consisting of water, methanol, ethanol, acetone, ethyl acetate, 1,3-buthylene glycol, hexane, diethyl ether, normal propanol. iso-propanol, and normal butanol.

Description

A cosmetic composition containing the extract of Injinho {A cosmetic composition coantaining Artemisiae capillaris Herba extracts}

The present invention relates to a cosmetic composition containing an extract of Asteraceae, Artemisiae capillaris Herba.

In the cosmetic field, oxidative stress caused by free radicals is the most important problem to overcome in order to suppress skin aging. Damage caused by free radicals is widely recognized in the human body and is recognized as a main mechanism of aging.

Free radicals are elements or molecules that have one or more unpaired electrons, and have the property of stabilizing electrons from other molecules. The lifetime of free radicals is very short (a few thousandths of a second), unstable and very aggressive. Radicals that are involved in aging of the skin include hydroxyl radical, superoxide radical, superoxide radical, singlet oxygen, hydrogen peroxide (H ₂ O ₂ ), and the like. These radicals have strong oxidative power and can react with all substances in the living body. Lipids are attacked to produce lipid peroxides, which alter the function of cell membranes, attack proteins to denature or lose their function, and attack DNA, a genetic information material. Continued exposure to oxidative stress caused by these radicals can lead to skin aging, sunburn and inflammation caused by sunlight, contact dermatitis, and wrinkles.

The human body is equipped with a defense system to prevent damage caused by free radicals. Vitamin E, vitamin C, Superoxide dismutase (SOD), catalase (Catalase) and various kinds of oxidase (Oxidase) and the like to eliminate free radicals. However, modern people are exposed to the harmful environment of air pollution, unhealthy diet, UV exposure, stress and disease, so that it is difficult to sufficiently eliminate oxidative stress caused by various radicals. Therefore, the substance capable of eliminating free radicals may prevent or delay aging of the skin by removing oxidative stress on the skin.

Therefore, the present inventors conducted a research study on herbal medicines growing in Korea in order to develop free radical scavenging substance from plants, and found that the free radical scavenging effect of Injinho extract was excellent.

Therefore, an object of the present invention is to provide a cosmetic composition containing the extract of Injinho.

The present invention relates to a cosmetic composition containing the extract of Artemisiae capillaris Herba.

Injinho extract, which is characteristically contained in the cosmetic composition according to the present invention, is extracted from asteraceae, Injinho (Artemisia capillaris Thunberg).

Asteraceae include Artemisia scoparia Waldst. Et Kit and Injinho (Artemisia capillaris Thumberg). Injinho is a perennial plant that grows in various parts of Korea. The characteristics of Injinho are stems are columnar, branched, 30cm to 100cm long, and 2mm to 8mm in diameter. The surface is light purple or purple with vertical stripes and covered with short soft hairs. Light and weak, white in cross section. The leaves are dense or mostly fall off. When young shoots grow about 10cm in spring, they are harvested and dried in the sun.

Injinho extract contained in the composition according to the present invention is water, methanol, methanol aqueous solution, ethanol ethanol aqueous solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butylene glycol aqueous solution, Hexane, diethyl ether, normal propanol, iso-propanol or normal butanol can be extracted using an extraction solvent and concentrated under reduced pressure.

The extract of Injinho may be prepared by immersing at 15 ° C. to 40 ° C. under the solvent for 1 to 15 days or extracting the extract from 1 to 7 days in an extractor equipped with a cooling capacitor, followed by concentration under reduced pressure. In addition, the extraction process may include a low-temperature aging process for 7 to 10 days at 4 to 15 ℃.

In the method of preparing the extract of Injinho, the extraction solvent is preferably used in an amount of 1 to 15 times the dry weight of Injinho.

Injinho extract prepared as described above may be prepared as a cosmetic composition by adding a conventional additive in a conventional method, it is preferably contained in an amount of 0.001 to 10 weight, more preferably 1 to 7 weight based on the dry amount of the cosmetic. .

The cosmetic composition according to the present invention may be prepared in the form of a basic cosmetic such as flexible lotion, milk lotion, nourishing cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil.

In addition, the cosmetic composition according to the present invention can be prepared in the form of color cosmetics, such as foundation, lipstick, mascara or makeup base.

In addition, the cosmetic composition according to the present invention can be prepared in the form of a hair cosmetic such as shampoo, conditioner, hair conditioner or hair gel.

Hereinafter, the present invention will be described in more detail with reference to Examples, Experimental Examples, Comparative Examples, and Prescription Examples. However, the present invention is not limited thereto.

Example 1

After washing with purified water and drying, 1 kg of powdered Injinho powder was placed in 5 L of water, extracted at 20 ° C. for 5 days, filtered through 300 mesh filter cloth, left at 10 ° C. for 10 days, and aged at low temperature. Filtered. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and dried to prepare 88.3 g (dry weight).

Example 2

After washing with purified water and drying, 1 kg of powdered Injinho powder was placed in 5 liters of 25 ethanol, and extracted for 3 days in an extractor equipped with a cooling condenser. Filter by filter paper. The extract was concentrated under reduced pressure to 60 ℃ in a distillation apparatus with a cooling condenser and dried to prepare 121.6g (dry weight).

Examples 3-15

To extract the dry weight by the method of Example 2 using a solvent described in Table 1 below as described in Table 1.

division menstruum Dry weight (unit: g) Example 3 50 ethanol 143.2 Example 4 75 Ethanol 154.7 Example 5 100 Ethanol 148.5 Example 6 80 Methanol 173.2 Example 7 100 Methanol 145.4 Example 8 Acetone 57.2 Example 9 Ethyl acetate 36.5 Example 10 501,3-butylene glycol aqueous solution 159.1 Example 11 Hexane 14.8 Example 12 Diethyl ether 25.7 Example 13 Normal propanol 52.6 Example 14 Isopropanol 54.3 Example 15 Normal butanol 49.2

Experimental Example 1

Free Radical Scavenging Effect of Injinho Extract

1) Experiment Method

The free radical scavenging effect was obtained by dimethyl sulfoxide of Injinho extract in 2 ml of 60 µM 1,1-diphenyl-2-picryl hydrazyl (DPPH) ethanol solution. 2 ml of DMSO) solution was added and reacted at room temperature for 30 minutes, and then the absorbance was measured at 520 nm to determine the free radical scavenging effect (). The free radical scavenging effect of each example extract was calculated by the following formula.

Free radical scavenging effect () = 100-[(absorbance after extract treatment / control absorbance) × 100]

2) Experiment result

The free radical scavenging effect of Injinho extract is shown in Table 2. Injinho extracts showed high activity.

Injinho Extract Free radical scavenging effect () Experimental concentration (㎍ / ㎖) 10 50 100 Example 1 12.5 45.5 67.8 Example 2 18.7 62.1 74.1 Example 3 28.7 82.4 87.5 Example 4 32.9 88.3 92.5 Example 5 29.8 80.6 85.4 Example 6 33.2 87.9 91.3 Example 7 30.1 82.4 58.7 Example 8 32.7 88.1 90.8 Example 9 35.6 89.8 94.2 Example 10 31.5 86.2 90.3 Example 11 13.1 38.4 55.7 Example 12 14.2 37.3 50.5 Example 13 22.5 57.9 78.2 Example 14 20.6 60.2 75.6 Example 15 23.8 68.4 82.2

* The above value is the average of 3 runs

Comparative Example 1

Comparison of Free Radical Scavenging Effect between Injinho Extract and Existing Free Radical Scavengers

Injinho extract was prepared in Example 6. The free radical scavenging effect of Injinho extract and conventional scavengers was measured in the same manner as in Experimental Example 1, and the results of comparing SC ₅₀ values are shown in Table 3. SC ₅₀ is the concentration of sample required to remove 50 free radicals.

Test substance SC ₅₀ (μg / ml) Injinho Extract (Example 6) 16.30 Ascorbic acid 29.74 Dibutyl hydroxyl toluene (BHT) 38.05 DL-α-Tocopherol 34.67 Beta-carotene 54.01 Morin 23.61

Comparing the free radical scavenging effect of Injinho extract with existing scavengers, Injinho extract showed very high activity.

Experimental Example 2

Inhibitory Effect of Injinho Extract on Hydrogen Peroxide (H ₂ O ₂ ) -induced Cytotoxicity

Inhibitory effect of Injinho extract on hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity was measured using mouse-derived skin fibroblasts. Hydrogen peroxide produces hydroxyl radicals in the cell, causing fatal damage to the cell. The free radical scavenging effect of Injinho extract was measured as follows to test at the cellular level.

1) Experiment Method

Injinho extract was prepared in Example 6. Mouse-derived fibroblasts (fibroblast, NIH / 3T3) were inoculated into each well (5 × 10 ⁴ ) in 96 well plates and incubated in 200 μl of Dulbecco's Modified Eagle Medium (DMEM) medium for 24 hours. 10 μl of H ₂ O ₂ diluted to a final concentration of 1 mM in the medium was added, and a 1,3-butylene glycol (50v / v, aqueous solution) solution of Injinho Extract was added at a final concentration of 0, 10, 20, 50 , 100 ㎍ / ㎖ and incubated for 24 hours, 10 μl of MTT solution (tetrazolium salt 3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) per well was added and left for 4 hours. Discarded, part of the badge. 100 μl of dimethyl sulfoxide (DMSO) solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.

2) Experiment result

Table 4 shows the inhibitory effect of Injinho extract on hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity. The cytotoxic effect was calculated by the following formula.

Cytotoxic inhibitory effect () = [(absorbance at extract treatment-absorbance at H ₂ O ₂ treatment) / absorbance at H ₂ O ₂ treatment] × 100

Concentration of Injinho Extract (㎍ / ㎖) Cytotoxic effect () 0 0 10 29.5 20 33.8 50 32.4 100 35.2

* The above value is the average of 3 runs

As shown in the above results, Injinho extract showed an inhibitory effect on hydrogen peroxide (H ₂ O ₂ ) -induced cytotoxicity. Therefore, Injinho extract was shown to suppress cytotoxicity by scavenging hydroxyl radicals generated in fibroblasts.

Experimental Example 3

Cytotoxicity of Injinho Extract

Cytotoxicity of Injinho extract itself was measured using mouse-derived skin fibroblasts (NIH / 3T3).

1) Experiment Method

Injinho extract was prepared in Example 6 was used.

Mouse-derived fibroblasts were inoculated with 5 × 10 ⁴ cells per well into a 96-well plate and cultured in 200 μl of DMEM medium. ㎖ was added and incubated for 24 hours. After 10 μl of MTT solution was added and left for 4 hours, 100 μl of dimethyl sulfoxide (DMSO) was added to the wells, dissolved for 20 minutes, and the absorbance was measured at 570 nm using a microplate reader.

2) Result of experiment

The cytotoxicity of Injinho extract itself is shown in Table 5. Cytotoxicity was calculated according to the following formula as cell viability.

Cell viability () = (absorbance at extract treatment / absorbance of control) × 100

Concentration of Injinho Extract (㎍ / ㎖) Cell viability () 0 100 50 99.65 100 97.23 200 92.68 500 86.84

* The above value is the average of 3 runs

Prescription Example 1

A prescription example of the flexible lotion among the cosmetics containing Injinho extract is as follows.

ingredient content Injinho Extract 5.0 1,3-butylene glycol 6.0 Sodium hyaluronate 2.0 glycerin 4.0 Fiji 4000 1.0 Poly Solvent 20 0.5 ethanol 10.0 antiseptic Quantity Benzophenone-9 0.05 incense Quantity Purified water Quantity Sum 100

Prescription Example 2

A prescription example of milk lotion among cosmetics containing Injinho extract is as follows.

ingredient Content (weight) Injinho Extract 5.0 Stearic acid 0.4 1,3-butylene glycol 6.0 Cetostearyl alcohol 1.2 glycerin 4.0 Glyceryl Stearate 1.0 Triethanol amine 0.25 Tocopheryl acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Poly Solvent 60 1.5 Solbitan Sesquioleate 0.6 Carboxyvinyl Polymer 0.15 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 3

Prescription example of nutritional cream in cosmetics containing Injinho extract is as follows.

ingredient Content (weight) Injinho Extract 5.0 vaseline 7.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 2.0 Stearic acid 1.5 Floating paraffin 10.0 Beeswax 2.0 Poly Solvent 60 1.5 Solbitan Sesquioleate 0.8 Squalane 3.0 1,3-butylene glycol 6.0 glycerin 4.0 Triethanol amine 0.5 Cotoperyl acetate 0.1 antiseptic Quantity incense Quantity Purified water Remaining amount Sum 100

Prescription Example 4

Prescription example of the essence in the cosmetic containing the extract of Injinho is as follows.

ingredient Content (weight) Injinho Extract 5.0 glycerin 10.0 Fiji 1500 2.0 Allantoin 0.1 Panthenol 0.3 EDTA 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer 0.2 Triethanolamine 0.18 Octyldodeceth-25 0.6 ethanol 6.0 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

Prescription Example 5

A prescription example of a massage cream in cosmetics containing Injinho extract is as follows.

ingredient Content (weight) Injinho Extract 3.0 Glyceryl Stearate 2.0 Cetostearyl alcohol 2.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 Squalene 5.0 Mineral oil 35.0 Dimethicone 1.0 Xanthan Gum 0.1 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanol amine 0.5 Preservative, fragrance, coloring Quantity Purified water Remaining amount Sum 100

Prescription Example 6

Examples of pack prescription among cosmetics containing Injinho extract are as follows.

ingredient Content (weight) Injinho Extract 3.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 Fiji 1500 2.0 Panthenol 0.4 Allantoin 0.1 ethanol 6.0 Fiji 40 Cured Castor Oil 0.3 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

As can be seen from the above, the Injinho extract according to the present invention can be effectively used in cosmetic compositions because it has a very good free radical scavenging effect and almost no cytotoxicity.

Claims (7)

Cosmetic composition containing the extract of Artemisiae capillaris Herba. The cosmetic composition according to claim 1, wherein the extract of Injinho is contained in an amount of 0.001 to 10% by weight based on the dry weight of the cosmetic. The method according to claim 1, wherein the extract of jinjinho is water, methanol and methanol aqueous solution, ethanol and ethanol aqueous solution, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butylene glycol aqueous solution, hexane, diethyl ether, normal Cosmetic composition, characterized in that extracted with an extraction solvent selected from the group consisting of propanol, iso-propanol and normal butanol. The cosmetic composition according to claim 1, which is a basic cosmetic, color cosmetic or hair cosmetic. 5. The cosmetic composition according to claim 4, wherein the basic cosmetic is softener, milk crocion, nutrition cream, massage cream, essence, cleansing foam, cleansing water, pack, or body oil. The cosmetic composition according to claim 4, wherein the color cosmetic is a foundation, lipstick, mascara or makeup base. The cosmetic composition according to claim 4, wherein the cosmetic for hair is a shampoo, a rinse, a hair conditioner or a hair gel.